From akemiat3377 at gmail.com Wed Nov 1 06:47:03 2017 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Wed, 1 Nov 2017 04:47:03 -0700 Subject: [Histonet] Supervising a histologist Message-ID: <59184896-AC5F-4D7C-BAD9-76EFE483D082@gmail.com> Hello Histoland: This may sound like a strange question, but I have my reasons. Has anyone run into a situation where a OJT histology assistant supervises an HTL when the histology manager is absent? Is it even legal? Any feedback would be greatly appreciated. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 From saby_joseph_a at yahoo.com Wed Nov 1 07:43:21 2017 From: saby_joseph_a at yahoo.com (Joseph Saby) Date: Wed, 1 Nov 2017 12:43:21 +0000 (UTC) Subject: [Histonet] Supervising a histologist In-Reply-To: <59184896-AC5F-4D7C-BAD9-76EFE483D082@gmail.com> References: <59184896-AC5F-4D7C-BAD9-76EFE483D082@gmail.com> Message-ID: <1397955145.353960.1509540201461@mail.yahoo.com> Eileen- I think it would depend on what specific procedures both of them are signed off on.And it would be on specific procedures.Or things are very strange in that area of Histoland. Joe Saby From: Eileen Akemi Allison via Histonet To: Histonet Sent: Wednesday, November 1, 2017 8:20 AM Subject: [Histonet] Supervising a histologist Hello Histoland: This may sound like a strange question, but I have my reasons.? Has anyone run into a situation where a OJT histology assistant supervises an HTL when the histology manager is absent?? Is it even legal?? Any feedback would be greatly appreciated. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 at gmail.com Wed Nov 1 11:12:05 2017 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Wed, 1 Nov 2017 09:12:05 -0700 Subject: [Histonet] Supervising a histologist In-Reply-To: <1397955145.353960.1509540201461@mail.yahoo.com> References: <59184896-AC5F-4D7C-BAD9-76EFE483D082@gmail.com> <1397955145.353960.1509540201461@mail.yahoo.com> Message-ID: <65BF4DDA-EE6E-4023-A7DC-C8B01741AD36@gmail.com> We are CLIA/CMS certified only. My lab assistant only has a high school degree and never had any background in any sciences. She knows nothing about trouble-shooting, nor theory and practice. She has worked primarily in clerical roles for our GI practice for 13 years. In the past 1.5 years, I trained her to accession, process, embed, cut, stain, and coverslip. We do not do IHC here. Our CEO/GI, MD said she could oversee our new HTL during my absence. Akemi Allison > On Nov 1, 2017, at 5:43 AM, Joseph Saby wrote: > > Eileen- > > I think it would depend on what specific procedures both of them are signed off on. > And it would be on specific procedures. > Or things are very strange in that area of Histoland. > > Joe Saby > > > From: Eileen Akemi Allison via Histonet > To: Histonet > Sent: Wednesday, November 1, 2017 8:20 AM > Subject: [Histonet] Supervising a histologist > > Hello Histoland: > > This may sound like a strange question, but I have my reasons. Has anyone run into a situation where a OJT histology assistant supervises an HTL when the histology manager is absent? Is it even legal? Any feedback would be greatly appreciated. > > > Akemi Allison BS, HT/HTL (ASCP) > Pathology Manager > Monterey Bay GI Consultants Laboratory > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From teri.johnson at navigatebp.com Wed Nov 1 12:14:30 2017 From: teri.johnson at navigatebp.com (Johnson, Teri) Date: Wed, 1 Nov 2017 17:14:30 +0000 Subject: [Histonet] Kappa & Lambda ISH Message-ID: <813f2753cc7047639a523be568ccccf9@BN1PR62MB038.023d.mgd.msft.net> Hi Renee, Are you using two different fixatives for bone marrow vs lymph node? And, if yes, have the assays been validated on both fixatives? Teri Johnson, HT(ASCP)QIHC Manager, Clinical Trial Testing T +1 760 516 5954 teri.johnson at navigatebp.com Navigate BioPharma, Inc. A Novartis Subsidiary 1890 Rutherford Rd. Carlsbad, CA? 92008 USA From tbraud at holyredeemer.com Wed Nov 1 12:40:42 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 1 Nov 2017 17:40:42 +0000 Subject: [Histonet] Supervising a histologist Message-ID: <48E053DDF6CE074DB6A7414BA05403F84CE999F2@HRHEX03-HOS.holyredeemer.local> I don't think it sounds like a strange question at all, but the quick answer is yes, it is perfectly legal and acceptable for an OJT Lab Assistant to be temporarily assigned to supervise an HTL. As long as it does not break any local/state requirements for licensure, or in the case of a unionized lab, and even then, it might be permissible since it is only a temporary "assignment" of responsibility in the absence of the manager/supervisor. Usually such a temporary position has less to do with technical expertise than a chain of command for reporting incidents, or assigning coverage in the absence of employees. I was once made a interim director of all lab services over MTs and MLTs, though I have no clinical experience. My main skill was communication and prioritization of work which served the position well. In times of trouble, I depended on the MTs./MLTs and the pathologists to give guidance for technical expertise. I would think that the same principles would apply. Sincerely, Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 2. Supervising a histologist (Eileen Akemi Allison) From: Eileen Akemi Allison via Histonet To: Histonet Sent: Wednesday, November 1, 2017 8:20 AM Subject: [Histonet] Supervising a histologist Hello Histoland: This may sound like a strange question, but I have my reasons.? Has anyone run into a situation where a OJT histology assistant supervises an HTL when the histology manager is absent?? Is it even legal?? Any feedback would be greatly appreciated. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 From nelsonrnch at verizon.net Wed Nov 1 14:54:43 2017 From: nelsonrnch at verizon.net (Patti Nelson - PN Lab Consultant) Date: Wed, 1 Nov 2017 15:54:43 -0400 Subject: [Histonet] Supervising a histologist In-Reply-To: <65BF4DDA-EE6E-4023-A7DC-C8B01741AD36@gmail.com> Message-ID: <15f79266d2b-c0a-3868f@webjas-vaa054.srv.aolmail.net> As long as the CEO/MD are on sight at all times while she is doing her duties. They need to be on sight accessible for your assistant.She will also need a Designee statement signed by the CEO/MD and herself that states what she is responsible for. She is not allowed to GROSS. Other than that I believe that she can over see the lab while you are gone. Sincerely, PATTI NELSON H.T.(ASCP) PN LABORATORY CONSULTANTS SUPERVISOR DGC/ZADEH LABS PO BOX 412 CABAZON, CA. 92230 909-841-9761 nelsonrnch at verizon.net CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender ofthe delivery error by e-mail or you may call 909-841-9761. -----Original Message----- From: Eileen Akemi Allison via Histonet To: Joseph Saby Cc: Histonet Sent: Wed, Nov 1, 2017 9:15 am Subject: Re: [Histonet] Supervising a histologist We are CLIA/CMS certified only. My lab assistant only has a high school degree and never had any background in any sciences. She knows nothing about trouble-shooting, nor theory and practice. She has worked primarily in clerical roles for our GI practice for 13 years. In the past 1.5 years, I trained her to accession, process, embed, cut, stain, and coverslip. We do not do IHC here. Our CEO/GI, MD said she could oversee our new HTL during my absence. Akemi Allison > On Nov 1, 2017, at 5:43 AM, Joseph Saby wrote: > > Eileen- > > I think it would depend on what specific procedures both of them are signed off on. > And it would be on specific procedures. > Or things are very strange in that area of Histoland. > > Joe Saby > > > From: Eileen Akemi Allison via Histonet > To: Histonet > Sent: Wednesday, November 1, 2017 8:20 AM > Subject: [Histonet] Supervising a histologist > > Hello Histoland: > > This may sound like a strange question, but I have my reasons. Has anyone run into a situation where a OJT histology assistant supervises an HTL when the histology manager is absent? Is it even legal? Any feedback would be greatly appreciated. > > > Akemi Allison BS, HT/HTL (ASCP) > Pathology Manager > Monterey Bay GI Consultants Laboratory > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NRich at bmhsc.org Thu Nov 2 10:38:16 2017 From: NRich at bmhsc.org (Nina J. Rich) Date: Thu, 2 Nov 2017 15:38:16 +0000 Subject: [Histonet] cytospin prep Message-ID: <6a752849c6e94d3ca632f3d93b6e580f@bmhsc.org> Good morning, For all those that have to do cytology cytospins. We are using the cytospin 4 with the large square mega funnels. We have a problem with the specimen always washing off the slide. Is there anyone who can send me their processing procedure for this type of funnel, to include whether or not you centrifuge the specimen first. The small circular funnels do not wash. Any input would greatly be appreciated. Jean From generashun_net at yahoo.com Thu Nov 2 10:45:50 2017 From: generashun_net at yahoo.com (Robert Herrera) Date: Thu, 2 Nov 2017 15:45:50 +0000 (UTC) Subject: [Histonet] Needing a microwave processor In-Reply-To: References: Message-ID: <218586860.1035695.1509637550787@mail.yahoo.com> Hello Lab Professionals We are currently searching for a microwave processor to purchase; new or used, any leads? Robert?919-395-5144 Sent from Yahoo Mail for iPhone On Wednesday, November 1, 2017, 1:20 PM, histonet-request at lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to ??? histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. Re: Kappa & Lambda ISH (Greg Dobbin) ? 2. Supervising a histologist (Eileen Akemi Allison) ? 3. Re: Supervising a histologist (Joseph Saby) ? 4. Re: Supervising a histologist (Eileen Akemi Allison) ---------------------------------------------------------------------- Message: 1 Date: Tue, 31 Oct 2017 14:15:00 -0300 From: Greg Dobbin To: rfisher0126 at msn.com Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Kappa & Lambda ISH Message-ID: ??? Content-Type: text/plain; charset="UTF-8" Hello Renee, We do K & L by ISH on the Bond-III and BondMax with Refine detection without issue on any of the tissues we have run (GI, skin, BM, LN). We have a normal tonsil control section on every slide. Do you also run a positive control on every slide? How does it perform? Next step would be for me to share our protocol. Let me know if you would like me to send that to you. Cheers, Greg -- *Greg Dobbin, R.T.* Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE? ? C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 *Everything in moderation...even moderation itself**!* ------------------------------ Message: 2 Date: Wed, 1 Nov 2017 04:47:03 -0700 From: Eileen Akemi Allison To: Histonet Subject: [Histonet] Supervising a histologist Message-ID: <59184896-AC5F-4D7C-BAD9-76EFE483D082 at gmail.com> Content-Type: text/plain;??? charset=us-ascii Hello Histoland: This may sound like a strange question, but I have my reasons.? Has anyone run into a situation where a OJT histology assistant supervises an HTL when the histology manager is absent?? Is it even legal?? Any feedback would be greatly appreciated. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 ------------------------------ Message: 3 Date: Wed, 1 Nov 2017 12:43:21 +0000 (UTC) From: Joseph Saby To: Eileen Akemi Allison , ??? Histonet ??? Subject: Re: [Histonet] Supervising a histologist Message-ID: <1397955145.353960.1509540201461 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Eileen- I think it would depend on what specific procedures both of them are signed off on.And it would be on specific procedures.Or things are very strange in that area of Histoland. Joe Saby ? ? ? From: Eileen Akemi Allison via Histonet To: Histonet Sent: Wednesday, November 1, 2017 8:20 AM Subject: [Histonet] Supervising a histologist ? Hello Histoland: This may sound like a strange question, but I have my reasons.? Has anyone run into a situation where a OJT histology assistant supervises an HTL when the histology manager is absent?? Is it even legal?? Any feedback would be greatly appreciated. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ------------------------------ Message: 4 Date: Wed, 1 Nov 2017 09:12:05 -0700 From: Eileen Akemi Allison To: Joseph Saby Cc: Histonet Subject: Re: [Histonet] Supervising a histologist Message-ID: <65BF4DDA-EE6E-4023-A7DC-C8B01741AD36 at gmail.com> Content-Type: text/plain;??? charset=us-ascii We are CLIA/CMS certified only.? My lab assistant only has a high school degree and never had any background in any sciences. She knows nothing about trouble-shooting, nor theory and practice. She has worked primarily in clerical roles for our GI practice for 13 years. In the past 1.5 years, I trained her to accession, process, embed, cut, stain, and coverslip. We do not do IHC here. Our CEO/GI, MD said she could oversee our new HTL during my absence. Akemi Allison > On Nov 1, 2017, at 5:43 AM, Joseph Saby wrote: > > Eileen- > > I think it would depend on what specific procedures both of them are signed off on. > And it would be on specific procedures. > Or things are very strange in that area of Histoland. > > Joe Saby > > > From: Eileen Akemi Allison via Histonet > To: Histonet > Sent: Wednesday, November 1, 2017 8:20 AM > Subject: [Histonet] Supervising a histologist > > Hello Histoland: > > This may sound like a strange question, but I have my reasons.? Has anyone run into a situation where a OJT histology assistant supervises an HTL when the histology manager is absent?? Is it even legal?? Any feedback would be greatly appreciated. > > > Akemi Allison BS, HT/HTL (ASCP) > Pathology Manager > Monterey Bay GI Consultants Laboratory > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 168, Issue 1 **************************************** From jwwalker at rrmc.org Thu Nov 2 11:03:15 2017 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Thu, 2 Nov 2017 16:03:15 +0000 Subject: [Histonet] cytospin prep In-Reply-To: <6a752849c6e94d3ca632f3d93b6e580f@bmhsc.org> References: <6a752849c6e94d3ca632f3d93b6e580f@bmhsc.org> Message-ID: Hi Nina, How are the cytology specimens collected? This will play a role in how you process them for evaluation on the Cytospin machine. We do not utilize the mega funnels. However, cytology specimens should be centrifuge first to remove any supernatant. Then equal parts of the Cytospin collection fluid should be added to the cell button and the cell button should be resuspended. Once resuspended, the amount of specimen that you put into the mega funnel chamber will vary depending on the cellularity of the cytology specimen. Large cell buttons might require less while small buttons will require more. If you over load the chamber, you will experience the cell spot/square falling off the slide when staining. This assumes that you are Pap staining your slides, hence the use of the Cytospin collection fluid. If you are making airdried specimens for Romanowsky stains, if you over load the chamber, you will have the same result of the spot falling of the slide. The manufacturer should provide max volume limits for the mega funnels but again a very cellular specimen will create issues. This has been our experience with cytospins. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewalker at rrmc.org, www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Our Commitment to our Community: We Listen, We Respect, We Care . . . Always. Joint Commission Accredited | Best Regional Hospital: U.S. News & World Report | Leapfrog Hospital Safety A Rating ANCC Magnet Hospital Designation(r) | Healthgrades: Excellence Award for Patient Safety | Healthgrades: Outstanding Patient Experience Award -----Original Message----- From: Nina J. Rich via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, November 02, 2017 11:38 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] cytospin prep Good morning, For all those that have to do cytology cytospins. We are using the cytospin 4 with the large square mega funnels. We have a problem with the specimen always washing off the slide. Is there anyone who can send me their processing procedure for this type of funnel, to include whether or not you centrifuge the specimen first. The small circular funnels do not wash. Any input would greatly be appreciated. Jean _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford at DignityHealth.org Thu Nov 2 11:48:50 2017 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Thu, 2 Nov 2017 16:48:50 +0000 Subject: [Histonet] Pathnet users Message-ID: <4c5c5f33cab54e739752753f9ef597a9@PHX-EXCH-013.chw.edu> Good Morning, We have Cerner Pathnet here and I need to pull three month's worth of IHC codes. 88341, 88342 and 88344 for billing purposes. We need to know how many IHC's we charged for the last three months and what they were. Does anyone know how to go about pulling this information out of this system? Thank you, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you From litepath2000 at yahoo.com Thu Nov 2 12:21:34 2017 From: litepath2000 at yahoo.com (LitePath) Date: Thu, 2 Nov 2017 17:21:34 +0000 (UTC) Subject: [Histonet] Searching 4 Antibody References: <596267429.1520824.1509643294132.ref@mail.yahoo.com> Message-ID: <596267429.1520824.1509643294132@mail.yahoo.com> Looking for recommendations/suggestions for anti-murine cytokeratin 13 for use in FFPE Thanks L From greg.dobbin at gmail.com Thu Nov 2 13:56:33 2017 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Thu, 2 Nov 2017 15:56:33 -0300 Subject: [Histonet] Kappa and Lambda by ISH Message-ID: Hi Folks, As it happens, we had a lymphoma case this week that was Kappa restricted (confirmed at a consulting laboratory by Flow Cytometry) and our Kappa and Lambda ISH staining was negative for both! In discussing it further with our Hematopathologist, our ISH probes for Kappa and Lambda in bone marrow specimens only stains plasma cells it does *NOT* stain B-cells. So this is likely why LN tend to be negative. Maybe one of our Histonet-contributing pathologists would like to elaborate? :-) Greg -- *Greg Dobbin* Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 *Everything in moderation...even moderation itself**!* From SteveM at mcclainlab.com Thu Nov 2 17:15:37 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Thu, 2 Nov 2017 22:15:37 +0000 Subject: [Histonet] Histonet Digest, Vol 168, Issue 2 Needing a microwave processor In-Reply-To: References: Message-ID: Why do you think you need a microwave processor? You can certainly use a conventional K3000 or VIP5 or RVG (and probably other) processors to accomplish short 2 hours-4 hours processing on small samples by 1. Raising the processing temperatures to 45-50C 2. Removing formalin from the processor (all fixation is done BEFORE loading on the processor) and 3.Maniacally ensuring your reagents are always fresh (frequent solution rotations) We looked to buy a microwave processor 10 years ago but found the equipment we had produced better results- identical to 8-12 hour overnight processing. This method has been posted several times on Histonet. Steve A. McClain, MD On Nov 2, 2017, at 10:14, "histonet-request at lists.utsouthwestern.edu" > wrote: Subject: Re: [Histonet] Needing a microwave processor From greg.dobbin at gmail.com Fri Nov 3 07:33:33 2017 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Fri, 3 Nov 2017 09:33:33 -0300 Subject: [Histonet] AFB Stain on Cytospin Preps Message-ID: Good day colleagues, We are occasionally being asked to do an acid-fast stain (Ziehl-Neelsen) on a alcohol-fixed cytospin preparation from a cytology fluid. Typically in Cytology, if a specimen is very bloody, we will add acetic acid to lyse the red cells. Does anyone know if acetic acid would have any adverse affect on the stainability of AFB organisms if present? Thank you, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From diane.satterfield at duke.edu Fri Nov 3 09:38:38 2017 From: diane.satterfield at duke.edu (Diane Satterfield) Date: Fri, 3 Nov 2017 14:38:38 +0000 Subject: [Histonet] LFB-H&E controls Message-ID: I am getting ready to learn how to do the LFB-H&E staining on brain tissue. I was wondering want I would use for a control with this staining. Diane From bcooper at chla.usc.edu Fri Nov 3 10:08:32 2017 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Fri, 3 Nov 2017 15:08:32 +0000 Subject: [Histonet] LFB-H&E controls In-Reply-To: References: Message-ID: We use spinal cord. -----Original Message----- From: Diane Satterfield via Histonet [histonet at lists.utsouthwestern.edu] Received: Friday, 03 Nov 2017, 7:39AM To: 'histonet at lists.utsouthwestern.edu' [histonet at lists.utsouthwestern.edu] Subject: [Histonet] LFB-H&E controls (EXTERNAL EMAIL) I am getting ready to learn how to do the LFB-H&E staining on brain tissue. I was wondering want I would use for a control with this staining. Diane _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From NRich at bmhsc.org Fri Nov 3 10:37:04 2017 From: NRich at bmhsc.org (Nina J. Rich) Date: Fri, 3 Nov 2017 15:37:04 +0000 Subject: [Histonet] cytospin prep In-Reply-To: References: <6a752849c6e94d3ca632f3d93b6e580f@bmhsc.org>, Message-ID: <445e4364af184bcc9370f7a9e7907f0e@bmhsc.org> Hey Joe, All the specimens come to the lab fresh. Instead of the Cytospin collection fluid we have been using thin prep preservcyt solution to resuspend, which is mostly methyl alcohol. Do you think that would have some influence to the washing problem. We are staining with the PAP stain. Thanks for your input let me know if you have anymore suggestions. Nina ________________________________________ From: Joe W. Walker, Jr. Sent: Thursday, November 2, 2017 12:03 PM To: Nina J. Rich; histonet at lists.utsouthwestern.edu Subject: RE: cytospin prep WARNING: This email originated outside of our company Beaufort Memorial DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe Hi Nina, How are the cytology specimens collected? This will play a role in how you process them for evaluation on the Cytospin machine. We do not utilize the mega funnels. However, cytology specimens should be centrifuge first to remove any supernatant. Then equal parts of the Cytospin collection fluid should be added to the cell button and the cell button should be resuspended. Once resuspended, the amount of specimen that you put into the mega funnel chamber will vary depending on the cellularity of the cytology specimen. Large cell buttons might require less while small buttons will require more. If you over load the chamber, you will experience the cell spot/square falling off the slide when staining. This assumes that you are Pap staining your slides, hence the use of the Cytospin collection fluid. If you are making airdried specimens for Romanowsky stains, if you over load the chamber, you will have the same result of the spot falling of the slide. The manufacturer should provide max volume limits for the mega funnels but again a very cellular specimen will create issues. This has been our experience with cytospins. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewalker at rrmc.org, www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Our Commitment to our Community: We Listen, We Respect, We Care . . . Always. Joint Commission Accredited | Best Regional Hospital: U.S. News & World Report | Leapfrog Hospital Safety A Rating ANCC Magnet Hospital Designation(r) | Healthgrades: Excellence Award for Patient Safety | Healthgrades: Outstanding Patient Experience Award -----Original Message----- From: Nina J. Rich via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, November 02, 2017 11:38 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] cytospin prep Good morning, For all those that have to do cytology cytospins. We are using the cytospin 4 with the large square mega funnels. We have a problem with the specimen always washing off the slide. Is there anyone who can send me their processing procedure for this type of funnel, to include whether or not you centrifuge the specimen first. The small circular funnels do not wash. Any input would greatly be appreciated. Jean _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague at Pathologyarts.com Fri Nov 3 11:00:52 2017 From: c.tague at Pathologyarts.com (Curt) Date: Fri, 3 Nov 2017 16:00:52 +0000 Subject: [Histonet] benchmark ultra bulks Message-ID: <9C8F910F72893643B3C3793C3D67132B68AE08E8@PATHOLOGYSERVER.pathologyarts.local> So like everyone else, I'm always trying to find new ways to save a few dollars, technology and costs are always increasing while reimbursements are always decreasing... With that, does anyone have any thoughts on alternative bulks to what Roche sells for the benchmark ultra, I'm thinking an alternative to their Ultra LCS... any thoughts? So long as a customer meets their monthly obligation it should be ok... Is it even possible... Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. From jwwalker at rrmc.org Fri Nov 3 11:15:45 2017 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Fri, 3 Nov 2017 16:15:45 +0000 Subject: [Histonet] cytospin prep In-Reply-To: <445e4364af184bcc9370f7a9e7907f0e@bmhsc.org> References: <6a752849c6e94d3ca632f3d93b6e580f@bmhsc.org>, <445e4364af184bcc9370f7a9e7907f0e@bmhsc.org> Message-ID: The PreservCyt solution is absolutely the culprit. The methanol and ethanol in that solution causes the cells to shrink and round up, thus creating your issues of them falling off during your staining protocol. The PreservCyt does a great job when utilized on the ThinPrep processor but not for your Cytospin application. The Cytospin fluid is probably cheaper than your PreservCyt too. I think if you switch to using the Cytospin fluid, you will see the end of your problems. The Cytospin fluid contains carbowax which aides in adhering the cells to the slide. Once your slides are made, allow them to completely air dry. Then you will want to soak them for at least 10 minutes in 95% ETOH to remove the carbowax layer prior to your Pap stain. You may need to increase this soak time to 15 minutes if you live in humid areas. Let me know if you need additional information, Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewalker at rrmc.org, www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Our Commitment to our Community: We Listen, We Respect, We Care . . . Always. Joint Commission Accredited | Best Regional Hospital: U.S. News & World Report | Leapfrog Hospital Safety A Rating ANCC Magnet Hospital Designation(r) | Healthgrades: Excellence Award for Patient Safety | Healthgrades: Outstanding Patient Experience Award -----Original Message----- From: Nina J. Rich [mailto:NRich at bmhsc.org] Sent: Friday, November 03, 2017 11:37 AM To: Joe W. Walker, Jr. ; histonet at lists.utsouthwestern.edu Subject: Re: cytospin prep Hey Joe, All the specimens come to the lab fresh. Instead of the Cytospin collection fluid we have been using thin prep preservcyt solution to resuspend, which is mostly methyl alcohol. Do you think that would have some influence to the washing problem. We are staining with the PAP stain. Thanks for your input let me know if you have anymore suggestions. Nina ________________________________________ From: Joe W. Walker, Jr. Sent: Thursday, November 2, 2017 12:03 PM To: Nina J. Rich; histonet at lists.utsouthwestern.edu Subject: RE: cytospin prep WARNING: This email originated outside of our company Beaufort Memorial DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe Hi Nina, How are the cytology specimens collected? This will play a role in how you process them for evaluation on the Cytospin machine. We do not utilize the mega funnels. However, cytology specimens should be centrifuge first to remove any supernatant. Then equal parts of the Cytospin collection fluid should be added to the cell button and the cell button should be resuspended. Once resuspended, the amount of specimen that you put into the mega funnel chamber will vary depending on the cellularity of the cytology specimen. Large cell buttons might require less while small buttons will require more. If you over load the chamber, you will experience the cell spot/square falling off the slide when staining. This assumes that you are Pap staining your slides, hence the use of the Cytospin collection fluid. If you are making airdried specimens for Romanowsky stains, if you over load the chamber, you will have the same result of the spot falling of the slide. The manufacturer should provide max volume limits for the mega funnels but again a very cellular specimen will create issues. This has been our experience with cytospins. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewalker at rrmc.org, www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Our Commitment to our Community: We Listen, We Respect, We Care . . . Always. Joint Commission Accredited | Best Regional Hospital: U.S. News & World Report | Leapfrog Hospital Safety A Rating ANCC Magnet Hospital Designation(r) | Healthgrades: Excellence Award for Patient Safety | Healthgrades: Outstanding Patient Experience Award -----Original Message----- From: Nina J. Rich via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, November 02, 2017 11:38 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] cytospin prep Good morning, For all those that have to do cytology cytospins. We are using the cytospin 4 with the large square mega funnels. We have a problem with the specimen always washing off the slide. Is there anyone who can send me their processing procedure for this type of funnel, to include whether or not you centrifuge the specimen first. The small circular funnels do not wash. Any input would greatly be appreciated. Jean _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From allanvv at gmail.com Fri Nov 3 11:20:47 2017 From: allanvv at gmail.com (Allan Wang) Date: Fri, 3 Nov 2017 12:20:47 -0400 Subject: [Histonet] benchmark ultra bulks In-Reply-To: <9C8F910F72893643B3C3793C3D67132B68AE08E8@PATHOLOGYSERVER.pathologyarts.local> References: <9C8F910F72893643B3C3793C3D67132B68AE08E8@PATHOLOGYSERVER.pathologyarts.local> Message-ID: The LCS is fairly cheap in comparison to some other bulks and 650-010 is half the price of 650-210. I don't know if there's actually any difference between them. I think an alternate formulation for EZ Prep (or Discovery Wash), CC1 and especially CC2 would save more money. Allan On Fri, Nov 3, 2017 at 12:00 PM, Curt via Histonet < histonet at lists.utsouthwestern.edu> wrote: > So like everyone else, I'm always trying to find new ways to save a few > dollars, technology and costs are always increasing while reimbursements > are always decreasing... > With that, does anyone have any thoughts on alternative bulks to what > Roche sells for the benchmark ultra, I'm thinking an alternative to their > Ultra LCS... any thoughts? > So long as a customer meets their monthly obligation it should be ok... > > Is it even possible... > > Curt > > > CONFIDENTIALITY NOTE: The information transmitted, including attachments, > is intended only for the person(s) or entity to which it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of, or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this in error, please > contact the sender and destroy any copies of this information. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From c.tague at Pathologyarts.com Fri Nov 3 11:31:28 2017 From: c.tague at Pathologyarts.com (Curt) Date: Fri, 3 Nov 2017 16:31:28 +0000 Subject: [Histonet] benchmark ultra bulks In-Reply-To: References: <9C8F910F72893643B3C3793C3D67132B68AE08E8@PATHOLOGYSERVER.pathologyarts.local> Message-ID: <9C8F910F72893643B3C3793C3D67132B68AE099F@PATHOLOGYSERVER.pathologyarts.local> My service/field technician said the Ultra LCS is required for the IHC, not sure why but that?s what I was told. Curt From: Allan Wang [mailto:allanvv at gmail.com] Sent: Friday, November 03, 2017 9:21 AM To: Curt Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] benchmark ultra bulks The LCS is fairly cheap in comparison to some other bulks and 650-010 is half the price of 650-210. I don't know if there's actually any difference between them. I think an alternate formulation for EZ Prep (or Discovery Wash), CC1 and especially CC2 would save more money. Allan On Fri, Nov 3, 2017 at 12:00 PM, Curt via Histonet > wrote: So like everyone else, I'm always trying to find new ways to save a few dollars, technology and costs are always increasing while reimbursements are always decreasing... With that, does anyone have any thoughts on alternative bulks to what Roche sells for the benchmark ultra, I'm thinking an alternative to their Ultra LCS... any thoughts? So long as a customer meets their monthly obligation it should be ok... Is it even possible... Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From redrose297 at gmail.com Fri Nov 3 11:53:32 2017 From: redrose297 at gmail.com (warda hassan) Date: Fri, 03 Nov 2017 16:53:32 +0000 Subject: [Histonet] Cyto system Message-ID: Hello All Can anyone worked on Sure path system and Thin prep system as Liquid based Cytology give me their feedback as in which system is better and why? Thanks in advance Nice weekend From jwwalker at rrmc.org Fri Nov 3 12:28:34 2017 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Fri, 3 Nov 2017 17:28:34 +0000 Subject: [Histonet] Pathnet users In-Reply-To: <4c5c5f33cab54e739752753f9ef597a9@PHX-EXCH-013.chw.edu> References: <4c5c5f33cab54e739752753f9ef597a9@PHX-EXCH-013.chw.edu> Message-ID: Hi Karen, We utilize Cerner Pathnet too. Unfortunately, reporting from the system isn't easy, or at least it isn't for us. We are currently stuck with Discern Analytics (not the 2.0 version), which can provide you the number of these charges and potentially the types of IHCs you performed but this is dependent on how you built your processing tasks. If you need to cross walk the pathnet side to what you actually charged a patient from the charge services side, we had to create a custom report with CCL in order to get the information. I'd work with your cerner lab analyst who should be able to guide you. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewalker at rrmc.org, www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Our Commitment to our Community: We Listen, We Respect, We Care . . . Always. Joint Commission Accredited | Best Regional Hospital: U.S. News & World Report | Leapfrog Hospital Safety A Rating ANCC Magnet Hospital Designation(r) | Healthgrades: Excellence Award for Patient Safety | Healthgrades: Outstanding Patient Experience Award -----Original Message----- From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, November 02, 2017 12:49 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pathnet users Good Morning, We have Cerner Pathnet here and I need to pull three month's worth of IHC codes. 88341, 88342 and 88344 for billing purposes. We need to know how many IHC's we charged for the last three months and what they were. Does anyone know how to go about pulling this information out of this system? Thank you, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Douglas.Porter at sparrow.org Fri Nov 3 12:41:44 2017 From: Douglas.Porter at sparrow.org (Porter, Douglas) Date: Fri, 3 Nov 2017 17:41:44 +0000 Subject: [Histonet] Assigning cases to pathologists Message-ID: <86fb463c054e4743a7934bfb35e3901f@EXCHMBPVAS02.shs.org> Histonetters, I have been asked to find out how other institutions divide up cases to be given to your pathologists. We give the Hem. Path. and the Derm. Path their respective cases. So, how do you divide up the rest? Currently, we divide them with one pathologist getting the odd numbered cases and another getting the even numbered cases. There is one pathologist assigned to handle overflow on heavy days. Thanks is advance, Douglas A. Porter, HT (ASCP) Pathologist Assistant IT Coordinator Sparrow Laboratories Department of Pathology 2508 South Cedar Street Lansing, MI 48910-3138 ________________________________ CONFIDENTIALITY NOTICE: This email communication may contain private, confidential, or legally privileged information intended for the sole use of the designated and/or duly authorized recipient(s). If you are not the intended recipient or have received this email in error, please notify the sender immediately by email and permanently delete all copies of this email including all attachments without reading them. If you are the intended recipient, secure the contents in a manner that conforms to all applicable state and/or federal requirements related to privacy and confidentiality of such information. From Douglas.Porter at sparrow.org Fri Nov 3 14:48:39 2017 From: Douglas.Porter at sparrow.org (Porter, Douglas) Date: Fri, 3 Nov 2017 19:48:39 +0000 Subject: [Histonet] Assigning cases to pathologists In-Reply-To: <86fb463c054e4743a7934bfb35e3901f@EXCHMBPVAS02.shs.org> References: <86fb463c054e4743a7934bfb35e3901f@EXCHMBPVAS02.shs.org> Message-ID: <5fab1f1ac367415897b56adb4ac6fdc3@EXCHMBPVAS02.shs.org> Thanks for the great responses so far. I did forget to mention we average about 700 blocks/day and have 12 pathologists. Two are Hem. Path and two are Derm. Path. That leaves 8 to divide the rest, not taking into consideration tumor conferences, vacations and such. Currently the work is divided as follows: FS/On call/QA Surgical Odd# Overflow Surgical Even# Overflow Cytology Support #1 Support #2 Outreach Thanks again, Douglas A. Porter, HT (ASCP) Pathologist Assistant IT Coordinator Sparrow Laboratories Department of Pathology 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) douglas.porter at sparrow.org From: Porter, Douglas Sent: Friday, November 03, 2017 1:42 PM To: Histonet at lists.utsouthwestern.edu Cc: Porter, Douglas Subject: Assigning cases to pathologists Histonetters, I have been asked to find out how other institutions divide up cases to be given to your pathologists. We give the Hem. Path. and the Derm. Path their respective cases. So, how do you divide up the rest? Currently, we divide them with one pathologist getting the odd numbered cases and another getting the even numbered cases. There is one pathologist assigned to handle overflow on heavy days. Thanks is advance, Douglas A. Porter, HT (ASCP) Pathologist Assistant IT Coordinator Sparrow Laboratories Department of Pathology 2508 South Cedar Street Lansing, MI 48910-3138 ________________________________ CONFIDENTIALITY NOTICE: This email communication may contain private, confidential, or legally privileged information intended for the sole use of the designated and/or duly authorized recipient(s). If you are not the intended recipient or have received this email in error, please notify the sender immediately by email and permanently delete all copies of this email including all attachments without reading them. If you are the intended recipient, secure the contents in a manner that conforms to all applicable state and/or federal requirements related to privacy and confidentiality of such information. From MLashus at pathgroup.com Mon Nov 6 10:07:29 2017 From: MLashus at pathgroup.com (Mighnon Lashus) Date: Mon, 6 Nov 2017 16:07:29 +0000 Subject: [Histonet] Histology Supervisor Job Message-ID: We are currently looking for a Histology Supervisor at our derm lab in Atlanta, see information below. Histology Supervisor Location: Atlanta, GA JOB DESCRIPTION Schedule: Mon- Fri 11:00am-7:00pm JOB SUMMARY: Histology Supervisor directly supervises and coordinates the activities of the histology team including performing routine duties of histology including embedding, microtome, specials stains and related tasks. ESSENTIAL FUNCTIONS: * Support PathGroup's mission, vision, goals and management decisions. * Provide leadership and supervise/manage assigned department, make decisions, solve problems, develop and document procedures, and conduct and attend meetings. * Manage the employee hiring process including interviewing prospective employees, developing performance expectations, identifying essential functions and knowledge, skills and abilities required for applicable positions. * Responsible for new employee orientation processes, employee training and continuing education. * Manage employee performance by coaching, counseling, motivating, and evaluating employees on a continual basis. Implement disciplinary action as needed and in consultation with Human Resources. * Ensure effective employee relations by sustaining an ethical, non-discriminatory and safe work environment and establishing effective communication lines and methods. Identify and solve employee problems, manage conflict, and respond to grievances as needed. * Monitor and evaluate lab operations, workflow, and employees on an on-going basis and direct re-assignment of job duties or changes in processes/ workflow as needed. * Work closely with histology management team to optimize workflow, quality, efficiency, and productivity. * Properly embed all types of tissue and keep embedding area clean. * Properly section all types of paraffin embedded surgical specimens and keep microtome and workstation clean. * Perform all special stains and prepare all required solutions. * Assure that paraffin blocks and slides are filed properly. * Maintain quality control records on all equipment. Clean, change solutions and properly set all tissue processors and have basic maintenance knowledge. * Assure proper disposal and handling of hazardous waste to include xylene recycling (i.e., xylene, reagent alcohol, formaldehyde). * Ensure routine maintenance of equipment and adequate stock of supplies. * Maintain required departmental records and reports. * Assist in the development and implementation of departmental policies and procedures and maintain department procedure manuals. * Exercise all laboratory safety precautions and adhere to lab procedures as stated in procedure manuals. * Perform all job responsibilities in alignment with the industry's best security practices and regulatory guidelines to protect the confidentiality, integrity, and availability of protected health information and other sensitive company data. * Must be familiar with and abide by the Corporate Compliance Program and all corporate policies, including the Privacy and Security policies. JOB REQUIREMENTS EDUCATION & LICENSURE: * High school diploma or GED required. * Associates Degree or other qualified technical college two year certificate preferred. * ASCP certification in Histology preferred. * Maintains appropriate continuing education for certification. REQUIREMENTS: * Over one year supervisory experience preferred or completion of PathGroup management training program. * Over five years' experience within the field of Histology required. * Computer proficiency in MS Word, Excel, and Outlook required. Atlanta Dermatopathology is an equal opportunity employer. Please submit your resume to info at atldermpath.com, or by mail to: Atlanta Dermatopathology Attn: R. Clay, Office Manager 1901 Phoenix Boulevard, Suite 210 Atlanta, GA 30349 Mighnon Lashus, HT (ASCP) Laboratory Manager PathGroup 4071 S. Access Rd, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus at pathgroup.com Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup immediately at 615-562-9255. Thank you From srishan at mail.holyname.org Mon Nov 6 10:12:47 2017 From: srishan at mail.holyname.org (Nirmala Srishan) Date: Mon, 6 Nov 2017 11:12:47 -0500 Subject: [Histonet] Elastic stain Message-ID: Histonetters, Is there someone who can recommend a Elastic stain other than Verhoeff? Thanks Mala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From bcooper at chla.usc.edu Mon Nov 6 12:25:47 2017 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Mon, 6 Nov 2017 18:25:47 +0000 Subject: [Histonet] Type of Nitrile Gloves in your lab Message-ID: Hi, Does anyone out there use a brand of nitrile glove that they really like? The ones we use here are not so good. When we pull them on, the part closet to the wrist often tears off, leaving behind a nice blue rubber band. At least several gloves per box have tears in them before we even put them on. One of our Quality Managers asked us to come up with alternatives. Can you help me out? You can email me offline if you don't want to name particular brands for everyone to see. PLEASE HELP!!! Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From michael.gudo at morphisto.de Mon Nov 6 13:03:08 2017 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Mon, 6 Nov 2017 20:03:08 +0100 Subject: [Histonet] Elastic stain In-Reply-To: References: Message-ID: Dear Mama, there are several elastic stains which are as brilliant as Verhoeff. For a very quick and easy stain you can try: Van Gieson with Resorcinfuchsin or Resorcinfuchsin with Thiazin-picric-acid Also very interesting results can be generated with: Aldehydfuchsin-Tripple Stain and Mollier-Staining with Orcein You should find the protocols in standard literature like Bancroft or Romeis, but please allow that I insert some links to our webshop where you can buy these staining-kits ready-to-use: https://webshop.morphisto.de/faerbekit-elastica-nach-van-gieson.html https://webshop.morphisto.de/faerbekit-resorcinfuchsin-mit-thiazinrot-pikrinsaeure.html https://webshop.morphisto.de/faerbekit-aldehydfuchsin-dreifachfaerbung.html https://webshop.morphisto.de/faerbekit-vierfachfaerbung-nach-mollier.html If you have any further questions, please just contact us via email. With best regards Michael > Am 06.11.2017 um 17:12 schrieb Nirmala Srishan via Histonet : > > Histonetters, > > Is there someone who can recommend a Elastic stain other than Verhoeff? > > Thanks > Mala > > > > > > > > > > Holy Name Medical Center is ranked among the top hospitals in the nation > for patient care, clinical performance and workplace excellence. > Click here to learn more. > > **** Warning: The information contained in this message is privileged and > CONFIDENTIAL and is intended only for the use of the addressee above. If > you are not the intended recipient, you are hereby notified that any > disclosure, copying, distribution, or taking of any action in reliance on > the content of this message is strictly prohibited. If you have received > this communication in error, please notify the sender by replying to this > message, and then delete it from your system. > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO Evolutionsforschung und Anwendung GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From llewllew at shaw.ca Mon Nov 6 14:42:37 2017 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Mon, 6 Nov 2017 12:42:37 -0800 Subject: [Histonet] Elastic stain In-Reply-To: References: Message-ID: <62844cbc-a719-7a74-03ed-ae2ddba1a518@shaw.ca> Please read the article at :http://stainsfile.info/StainsFile/stain/elastic/elastic.htm There are numerous alternatives discussed there. I particularly like the Humberstone variant of iron resorcin fuchsin. Bryan Llewellyn Nirmala Srishan via Histonet wrote: > Histonetters, > > Is there someone who can recommend a Elastic stain other than Verhoeff? > > Thanks > Mala > > > > > > > > > > Holy Name Medical Center is ranked among the top hospitals in the nation > for patient care, clinical performance and workplace excellence. > Click here to learn more. > > **** Warning: The information contained in this message is privileged and > CONFIDENTIAL and is intended only for the use of the addressee above. If > you are not the intended recipient, you are hereby notified that any > disclosure, copying, distribution, or taking of any action in reliance on > the content of this message is strictly prohibited. If you have received > this communication in error, please notify the sender by replying to this > message, and then delete it from your system. > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From doolee at shands.ufl.edu Mon Nov 6 16:13:28 2017 From: doolee at shands.ufl.edu (Dooley, Elaine) Date: Mon, 6 Nov 2017 22:13:28 +0000 Subject: [Histonet] Amyloid A controls Message-ID: Hello Histonetters, I am looking for a good "Amyloid A control". We have an anti-amyloid A antibody which is specific only for amyloid A. We have tried our commercial congo red controls and they do not work. We have only had a few positive cases. I am willing to trade control blocks. We have parvovirus, h,pylori, C4d on heart, and many other controls just ask and maybe we could make a trade. Elaine Dooley Shands Labs at Rocky Point 4800 SW 35th drive Room 1130 Histology Gainesville FL 32608 352-265-0111 ext 72117 From tony.henwood at health.nsw.gov.au Mon Nov 6 16:51:12 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon, 6 Nov 2017 22:51:12 +0000 Subject: [Histonet] Elastic stain In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF9559BE52@SVDCMBX-MEX008.nswhealth.net> I would recommend the Orcein stain: Henwood, A., (2003) "Current applications of orcein in histochemistry. A brief review with some new observations concerning influence of dye batch variation and aging of dye solutions on staining" Biotech Histochem. 78(6):303-8. And you can pair the orcein stain with several counterstains (Picro light green, Periodic acid Blue Schiff's, Perls, H&E etc.): Henwood, A.F., (2002) "The Orcein Stain: A versatile stain for Histopathology" J. Histotechn. 25(1):29-32. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Nirmala Srishan via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, 7 November 2017 3:13 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Elastic stain Histonetters, Is there someone who can recommend a Elastic stain other than Verhoeff? Thanks Mala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From pmdooley27 at gmail.com Tue Nov 7 07:30:39 2017 From: pmdooley27 at gmail.com (Patrick Dooley) Date: Tue, 7 Nov 2017 08:30:39 -0500 Subject: [Histonet] LFB-H&E controls Message-ID: Any well defined block of CNS with both grey and white matter would work as a control for an LHE. The differentiation is the tricky part; I've found looking at the projecting white matter tracts are the best way to tell that you've successfully differentiated the Luxol enough. Patrick Dooley, HTL(ASCP) Research Tech I / Tissue Bank Coordinator MGH Alzheimer?s Disease Resource Center CNY 114 Rm. 2650 617-643-6994(office) From lab at premierdermde.com Tue Nov 7 07:47:03 2017 From: lab at premierdermde.com (lab) Date: Tue, 7 Nov 2017 13:47:03 +0000 Subject: [Histonet] HPV Probes Message-ID: To All, I am looking for control tissue blocks or control slides for individual HPV probes. 6, 11, 16, 18, 31, 33. Does this exist to purchase? I have looked at a dozen companies and after some research they will come back with nothing. I have found high and low controls, but I am looking for individual controls. Thank you, in advance, for your time. From hhuggins at novalabs.co Tue Nov 7 10:21:35 2017 From: hhuggins at novalabs.co (Haley Huggins) Date: Tue, 7 Nov 2017 08:21:35 -0800 Subject: [Histonet] Type of Nitrile Gloves in your lab In-Reply-To: References: Message-ID: I like the Halyard brand. We also use Confiderm from McKesson, those are pretty good too. *Haley Huggins, HT (ASCP)cm* *Technical Lab Supervisor* *1050 Las Tablas Rd, Suite 14* *Templeton, CA 93465* *Office: 877-230-1518* On Mon, Nov 6, 2017 at 10:25 AM, Cooper, Brian via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi, > > Does anyone out there use a brand of nitrile glove that they really like? > The ones we use here are not so good. When we pull them on, the part > closet to the wrist often tears off, leaving behind a nice blue rubber > band. At least several gloves per box have tears in them before we even > put them on. One of our Quality Managers asked us to come up with > alternatives. Can you help me out? You can email me offline if you don't > want to name particular brands for everyone to see. PLEASE HELP!!! > > Thanks, > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 Pager: 213-209-0184 > bcooper at chla.usc.edu > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, > please > contact the sender by reply e-mail and destroy all copies of this original > message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Jose.R.deGuzman at gunet.georgetown.edu Tue Nov 7 14:01:48 2017 From: Jose.R.deGuzman at gunet.georgetown.edu (deGuzman, Jose R) Date: Tue, 7 Nov 2017 15:01:48 -0500 Subject: [Histonet] AFB Stain on Cytospin Preps In-Reply-To: References: Message-ID: Greg, I'm not sure if anyone has responded to your question, the waxy coat of acid-fast bacilli is sensitive to xylene and alcohol not to acid. Carbol Fuchsin is soluble in acid alcohol but if the waxy coat of the bacteria is intact, it will hold the stain. What I've suggested before is a cyto-spin preparation of unfixed fluid avoiding any alcohol or xylene even for dehydration and clearing. Hope this helps. J Good day colleagues, We are occasionally being asked to do an acid-fast stain (Ziehl-Neelsen) on a alcohol-fixed cytospin preparation from a cytology fluid. Typically in Cytology, if a specimen is very bloody, we will add acetic acid to lyse the red cells. Does anyone know if acetic acid would have any adverse affect on the stainability of AFB organisms if present? Thank you, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From mills at 3scan.com Tue Nov 7 19:01:29 2017 From: mills at 3scan.com (Caroline Miller) Date: Tue, 7 Nov 2017 17:01:29 -0800 Subject: [Histonet] Type of Nitrile Gloves in your lab In-Reply-To: References: Message-ID: I love these: *https://www.net32.com/ec/cobalt-nitrile-exam-gloves-xsmall-100-powderfree-d-135964 * *This is also the cheapest place I have found them listed as well. * *yours,* *mills* On Tue, Nov 7, 2017 at 8:21 AM, Haley Huggins via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I like the Halyard brand. We also use Confiderm from McKesson, those are > pretty good too. > > *Haley Huggins, HT (ASCP)cm* > *Technical Lab Supervisor* > *1050 Las Tablas Rd, Suite 14* > *Templeton, CA 93465* > *Office: 877-230-1518* > > On Mon, Nov 6, 2017 at 10:25 AM, Cooper, Brian via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hi, > > > > Does anyone out there use a brand of nitrile glove that they really like? > > The ones we use here are not so good. When we pull them on, the part > > closet to the wrist often tears off, leaving behind a nice blue rubber > > band. At least several gloves per box have tears in them before we even > > put them on. One of our Quality Managers asked us to come up with > > alternatives. Can you help me out? You can email me offline if you > don't > > want to name particular brands for everyone to see. PLEASE HELP!!! > > > > Thanks, > > > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > > Department of Pathology and Laboratory Medicine > > Children's Hospital Los Angeles > > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > > Ph: 323.361.3357 Pager: 213-209-0184 > > bcooper at chla.usc.edu > > > > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > > is for the sole use of the intended recipient(s) and may contain > > confidential > > or legally privileged information. Any unauthorized review, use, > disclosure > > or distribution is prohibited. If you are not the intended recipient, > > please > > contact the sender by reply e-mail and destroy all copies of this > original > > message. > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From tbraud at holyredeemer.com Wed Nov 8 08:31:44 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 8 Nov 2017 14:31:44 +0000 Subject: [Histonet] Elastic Stain Message-ID: <48E053DDF6CE074DB6A7414BA05403F84CE9AA0B@HRHEX03-HOS.holyredeemer.local> We love the Aldehyde Fuchsin with a fast-green counterstain. Once the initial working stain is made up, it is a fast and easy stain to perform, not to mention just pretty - purple fibers against a green background. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal ************************* From tbraud at holyredeemer.com Wed Nov 8 08:43:11 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 8 Nov 2017 14:43:11 +0000 Subject: [Histonet] Division of Path cases Message-ID: <48E053DDF6CE074DB6A7414BA05403F84CE9AA7E@HRHEX03-HOS.holyredeemer.local> We give the worklist to the chief Pathologist and let him make the assignments on a daily basis. They are usually divided up by complexity. In the absence of the Chief Path, then I make the assignments. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal ********************************* From redrose297 at gmail.com Wed Nov 8 09:28:56 2017 From: redrose297 at gmail.com (warda hassan) Date: Wed, 08 Nov 2017 15:28:56 +0000 Subject: [Histonet] mounting medium Message-ID: Hello to all Can anyone suggest which mouting medium is best that will help preservation of staining properties without creating fading of stains and bubbles on long run. Thanks alot From portera at msu.edu Wed Nov 8 12:53:37 2017 From: portera at msu.edu (Amy Porter) Date: Wed, 8 Nov 2017 13:53:37 -0500 Subject: [Histonet] NK Cell markers for Mouse in Paraffin Message-ID: <001301d358c2$e2c000d0$a8400270$@edu> Hi all - anyone out there have an antibody that they like for NK Cells in a mouse model - I am trying to work with a mouse monoclonal (MOM) with polymer technology. Looking for assistance from labs that have established protocols. We have tried enzymatic and heat retrieval ..not getting good results. Any comments would be appreciated - willing to change antibody vendors currently using clone PK136. Thanks - Amy Amy S. Porter, HT (ASCP) Michigan State University - Department of Physiology Investigative HistoPathology Lab - Supervisor Research Core Support Facility 567 Wilson Road - Room 2201 East Lansing, MI 48824-6458 517-884-5026 portera at msu.edu From diane.satterfield at duke.edu Wed Nov 8 13:42:09 2017 From: diane.satterfield at duke.edu (Diane Satterfield) Date: Wed, 8 Nov 2017 19:42:09 +0000 Subject: [Histonet] Metal embedding molds-large Message-ID: We are using large metal molds to embed mouse brains. We are having a hard time getting to block out of the molds, the paraffin blocks are sticking. Sometimes they are coming out cracked. Sometimes the cassette comes off the paraffin block. Any idea why this is happening? Any advice on how to fix this problem? Diane L. Satterfield, BS Manager Brain Tumor BioRepository Research Program Leader Duke University Medical Center Brain Tumor Center Biorepository and Database diane.satterfield at duke.edu office 919-684-4642 pager 919-970-7328 fax 919-684-4975 CONFIDENTIALITY NOTICE: The information contained in this electronic mail is sensitive, protected information intended only for the addressee(s). Any other person, including anyone who believes he/she might have received it due to an addressing error, is requested to notify the sender immediately by return electronic mail, and to delete it without further reading or retention. The information is not to be forwarded to or shared unless in compliance with Duke Medicine policies on confidentiality and/or with the approval of the sender. From jaylundgren at gmail.com Wed Nov 8 14:23:58 2017 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 8 Nov 2017 12:23:58 -0800 Subject: [Histonet] Metal embedding molds-large In-Reply-To: References: Message-ID: mold release Virus-free. www.avg.com <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> On Wed, Nov 8, 2017 at 11:42 AM, Diane Satterfield via Histonet < histonet at lists.utsouthwestern.edu> wrote: > We are using large metal molds to embed mouse brains. We are having a > hard time getting to block out of the molds, the paraffin blocks are > sticking. Sometimes they are coming out cracked. Sometimes the cassette > comes off the paraffin block. Any idea why this is happening? Any advice > on how to fix this problem? > > > Diane L. Satterfield, BS > Manager Brain Tumor BioRepository > Research Program Leader > Duke University Medical Center > Brain Tumor Center Biorepository and Database > > diane.satterfield at duke.edu > office 919-684-4642 > pager 919-970-7328 > fax 919-684-4975 > > CONFIDENTIALITY NOTICE: The information contained in this electronic mail > is sensitive, protected information intended only for the addressee(s). > Any other person, including anyone who believes he/she might have received > it due to an addressing error, is requested to notify the sender > immediately by return electronic mail, and to delete it without further > reading or retention. The information is not to be forwarded to or shared > unless in compliance with Duke Medicine policies on confidentiality and/or > with the approval of the sender. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From llewllew at shaw.ca Wed Nov 8 14:24:08 2017 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Wed, 8 Nov 2017 12:24:08 -0800 Subject: [Histonet] Metal embedding molds-large In-Reply-To: References: Message-ID: <11e6f002-2f10-63ac-8fa4-691bdbf847f7@shaw.ca> This used to be a common problem years ago. It is due to crud buildup on the metal. Boil them with TCP for half an hour, then thoroughly wash them in cold water. Coat them with a VERY light smear of glycerol before you use them, preferably each time. That should help. Bryan Llewellyn. Diane Satterfield via Histonet wrote: > We are using large metal molds to embed mouse brains. We are having a hard time getting to block out of the molds, the paraffin blocks are sticking. Sometimes they are coming out cracked. Sometimes the cassette comes off the paraffin block. Any idea why this is happening? Any advice on how to fix this problem? > > > Diane L. Satterfield, BS > Manager Brain Tumor BioRepository > Research Program Leader > Duke University Medical Center > Brain Tumor Center Biorepository and Database > > diane.satterfield at duke.edu > office 919-684-4642 > pager 919-970-7328 > fax 919-684-4975 > > CONFIDENTIALITY NOTICE: The information contained in this electronic mail is sensitive, protected information intended only for the addressee(s). Any other person, including anyone who believes he/she might have received it due to an addressing error, is requested to notify the sender immediately by return electronic mail, and to delete it without further reading or retention. The information is not to be forwarded to or shared unless in compliance with Duke Medicine policies on confidentiality and/or with the approval of the sender. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From llewllew at shaw.ca Wed Nov 8 14:30:06 2017 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Wed, 8 Nov 2017 12:30:06 -0800 Subject: [Histonet] Metal embedding molds-large In-Reply-To: <11e6f002-2f10-63ac-8fa4-691bdbf847f7@shaw.ca> References: <11e6f002-2f10-63ac-8fa4-691bdbf847f7@shaw.ca> Message-ID: Sorry! That should be TSP - trisodium phosphate - not TCP, which might make it worse. Bryan Bryan Llewellyn wrote: > This used to be a common problem years ago. It is due to crud buildup on > the metal. Boil them with TCP for half an hour, then thoroughly wash > them in cold water. Coat them with a VERY light smear of glycerol before > you use them, preferably each time. That should help. > > Bryan Llewellyn. > > Diane Satterfield via Histonet wrote: >> We are using large metal molds to embed mouse brains. We are having a >> hard time getting to block out of the molds, the paraffin blocks are >> sticking. Sometimes they are coming out cracked. Sometimes the >> cassette comes off the paraffin block. Any idea why this is >> happening? Any advice on how to fix this problem? >> >> >> Diane L. Satterfield, BS >> Manager Brain Tumor BioRepository >> Research Program Leader >> Duke University Medical Center >> Brain Tumor Center Biorepository and Database >> >> diane.satterfield at duke.edu >> office 919-684-4642 >> pager 919-970-7328 >> fax 919-684-4975 >> >> CONFIDENTIALITY NOTICE: The information contained in this electronic >> mail is sensitive, protected information intended only for the >> addressee(s). Any other person, including anyone who believes he/she >> might have received it due to an addressing error, is requested to >> notify the sender immediately by return electronic mail, and to delete >> it without further reading or retention. The information is not to be >> forwarded to or shared unless in compliance with Duke Medicine >> policies on confidentiality and/or with the approval of the sender. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From mills at 3scan.com Wed Nov 8 15:46:39 2017 From: mills at 3scan.com (Caroline Miller) Date: Wed, 8 Nov 2017 13:46:39 -0800 Subject: [Histonet] Metal embedding molds-large In-Reply-To: References: <11e6f002-2f10-63ac-8fa4-691bdbf847f7@shaw.ca> Message-ID: hey, we do all our embedding in those molds, and here is what I suggest: 1 - Make sure to have enough wax in the back of the molds, all the way until it is on the lip of the cassette - you may need to refill a bunch of times because the wax drains out (why regular sakura cassettes do not fit in these molds, also made by sakura I really don't know). But you need to wait until it hardens enough, but not too much to leave a transition between the two fill waxes. This will prevent the cassette from coming away when you pop it out 2 - Make sure the block is nice and cold, wait 10 minutes longer than you think you have to 3 - Use a spatula or other strong item to place under the label side of the cassette and pop out of the mold, if you get any resistance then WAIT some more! (again them being cold is really important) good luck, happy to answer any clarifying questions! mills On Wed, Nov 8, 2017 at 12:30 PM, Bryan Llewellyn via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Sorry! > > That should be TSP - trisodium phosphate - not TCP, which might make it > worse. > > Bryan > > > > Bryan Llewellyn wrote: > >> This used to be a common problem years ago. It is due to crud buildup on >> the metal. Boil them with TCP for half an hour, then thoroughly wash >> them in cold water. Coat them with a VERY light smear of glycerol before >> you use them, preferably each time. That should help. >> >> Bryan Llewellyn. >> >> Diane Satterfield via Histonet wrote: >> >>> We are using large metal molds to embed mouse brains. We are having a >>> hard time getting to block out of the molds, the paraffin blocks are >>> sticking. Sometimes they are coming out cracked. Sometimes the >>> cassette comes off the paraffin block. Any idea why this is >>> happening? Any advice on how to fix this problem? >>> >>> >>> Diane L. Satterfield, BS >>> Manager Brain Tumor BioRepository >>> Research Program Leader >>> Duke University Medical Center >>> Brain Tumor Center Biorepository and Database >>> >>> diane.satterfield at duke.edu >>> office 919-684-4642 >>> pager 919-970-7328 >>> fax 919-684-4975 >>> >>> CONFIDENTIALITY NOTICE: The information contained in this electronic >>> mail is sensitive, protected information intended only for the >>> addressee(s). Any other person, including anyone who believes he/she >>> might have received it due to an addressing error, is requested to >>> notify the sender immediately by return electronic mail, and to delete >>> it without further reading or retention. The information is not to be >>> forwarded to or shared unless in compliance with Duke Medicine >>> policies on confidentiality and/or with the approval of the sender. >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >> > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From mills at 3scan.com Wed Nov 8 15:48:13 2017 From: mills at 3scan.com (Caroline Miller) Date: Wed, 8 Nov 2017 13:48:13 -0800 Subject: [Histonet] Elastic Stain In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F84CE9AA0B@HRHEX03-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F84CE9AA0B@HRHEX03-HOS.holyredeemer.local> Message-ID: In the UK clinical lab I started in we used Miller's elastin, which I think was a resourcinol formulation (digging from 20 years ago in my brain). I tried to find that in the US but never did. I loved that stain, worked well every time! We would counterstain with a van-Giesen, looked stunning! the elastin came out very dark blue / black. mills On Wed, Nov 8, 2017 at 6:31 AM, Terri Braud via Histonet < histonet at lists.utsouthwestern.edu> wrote: > We love the Aldehyde Fuchsin with a fast-green counterstain. Once the > initial working stain is made up, it is a fast and easy stain to perform, > not to mention just pretty - purple fibers against a green background. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > ************************* > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From cormier at mit.edu Thu Nov 9 06:23:33 2017 From: cormier at mit.edu (Kathleen S Cormier) Date: Thu, 9 Nov 2017 12:23:33 +0000 Subject: [Histonet] Chrome Alum slides picking up eosin non specifically? Message-ID: <68218F88-E8FA-4D49-82D6-ECAB218B507C@mit.edu> Hello Everyone, Was wondering, I am making up some chrome alum slides, and they seem to pick up quite the bit of eosin non specifically. Is this typical? If not, what should I do differently? Thanks! Kathy Kathy Cormier cormier at mit.edu From kc at ka-recruiting.com Thu Nov 9 14:47:20 2017 From: kc at ka-recruiting.com (K.C. Carpenter) Date: Thu, 9 Nov 2017 15:47:20 -0500 Subject: [Histonet] Your hiring needs Message-ID: Hi Everyone - Please let me know if you have any open Histotech positions in your lab. My company is a healthcare recruiting firm specializing in helping labs fill their permanent positions and we're currently working with some great applicants who are looking to secure new positions before the end of the year. Thanks, K.C. Carpenter Director of Business Development, K.A. Recruiting, Inc. (o) 617-746-2741 (m) 646-221-5674 www.ka-recruiting.com From TNMayer at mdanderson.org Thu Nov 9 15:35:34 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Thu, 9 Nov 2017 21:35:34 +0000 Subject: [Histonet] Metal embedding molds-large (Diane Satterfield) Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC883A049FAF@D1PWPEXMBX08.mdanderson.edu> You could boil molds in soapy water, cool, remove paraffin, rinse and dry. You could place them in the processor on the clean cycle. You could soak them in xylene, rinse in 100% etoh. Milestone has a paraffin removal instrument that can be used as well. We just got one for our student lab and it is wonderful. Toysha Mayer -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, November 09, 2017 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 168, Issue 8 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. NK Cell markers for Mouse in Paraffin (Amy Porter) 2. Metal embedding molds-large (Diane Satterfield) 3. Re: Metal embedding molds-large (Jay Lundgren) 4. Re: Metal embedding molds-large (Bryan Llewellyn) 5. Re: Metal embedding molds-large (Bryan Llewellyn) 6. Re: Metal embedding molds-large (Caroline Miller) 7. Re: Elastic Stain (Caroline Miller) 8. Chrome Alum slides picking up eosin non specifically? (Kathleen S Cormier) ---------------------------------------------------------------------- Message: 1 Date: Wed, 8 Nov 2017 13:53:37 -0500 From: "Amy Porter" To: Subject: [Histonet] NK Cell markers for Mouse in Paraffin Message-ID: <001301d358c2$e2c000d0$a8400270$@edu> Content-Type: text/plain; charset="us-ascii" Hi all - anyone out there have an antibody that they like for NK Cells in a mouse model - I am trying to work with a mouse monoclonal (MOM) with polymer technology. Looking for assistance from labs that have established protocols. We have tried enzymatic and heat retrieval ..not getting good results. Any comments would be appreciated - willing to change antibody vendors currently using clone PK136. Thanks - Amy Amy S. Porter, HT (ASCP) Michigan State University - Department of Physiology Investigative HistoPathology Lab - Supervisor Research Core Support Facility 567 Wilson Road - Room 2201 East Lansing, MI 48824-6458 517-884-5026 portera at msu.edu ------------------------------ Message: 2 Date: Wed, 8 Nov 2017 19:42:09 +0000 From: Diane Satterfield To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Metal embedding molds-large Message-ID: Content-Type: text/plain; charset="us-ascii" We are using large metal molds to embed mouse brains. We are having a hard time getting to block out of the molds, the paraffin blocks are sticking. Sometimes they are coming out cracked. Sometimes the cassette comes off the paraffin block. Any idea why this is happening? Any advice on how to fix this problem? Diane L. Satterfield, BS Manager Brain Tumor BioRepository Research Program Leader Duke University Medical Center Brain Tumor Center Biorepository and Database diane.satterfield at duke.edu office 919-684-4642 pager 919-970-7328 fax 919-684-4975 CONFIDENTIALITY NOTICE: The information contained in this electronic mail is sensitive, protected information intended only for the addressee(s). Any other person, including anyone who believes he/she might have received it due to an addressing error, is requested to notify the sender immediately by return electronic mail, and to delete it without further reading or retention. The information is not to be forwarded to or shared unless in compliance with Duke Medicine policies on confidentiality and/or with the approval of the sender. ------------------------------ Message: 3 Date: Wed, 8 Nov 2017 12:23:58 -0800 From: Jay Lundgren To: Diane Satterfield Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Metal embedding molds-large Message-ID: Content-Type: text/plain; charset="UTF-8" mold release Virus-free. www.avg.com <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> On Wed, Nov 8, 2017 at 11:42 AM, Diane Satterfield via Histonet < histonet at lists.utsouthwestern.edu> wrote: > We are using large metal molds to embed mouse brains. We are having a > hard time getting to block out of the molds, the paraffin blocks are > sticking. Sometimes they are coming out cracked. Sometimes the > cassette comes off the paraffin block. Any idea why this is > happening? Any advice on how to fix this problem? > > > Diane L. Satterfield, BS > Manager Brain Tumor BioRepository > Research Program Leader > Duke University Medical Center > Brain Tumor Center Biorepository and Database > > diane.satterfield at duke.edu > office 919-684-4642 > pager 919-970-7328 > fax 919-684-4975 > > CONFIDENTIALITY NOTICE: The information contained in this electronic > mail is sensitive, protected information intended only for the addressee(s). > Any other person, including anyone who believes he/she might have > received it due to an addressing error, is requested to notify the > sender immediately by return electronic mail, and to delete it without > further reading or retention. The information is not to be forwarded > to or shared unless in compliance with Duke Medicine policies on > confidentiality and/or with the approval of the sender. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 4 Date: Wed, 8 Nov 2017 12:24:08 -0800 From: Bryan Llewellyn To: Diane Satterfield , Histonet Subject: Re: [Histonet] Metal embedding molds-large Message-ID: <11e6f002-2f10-63ac-8fa4-691bdbf847f7 at shaw.ca> Content-Type: text/plain; charset=UTF-8; format=flowed This used to be a common problem years ago. It is due to crud buildup on the metal. Boil them with TCP for half an hour, then thoroughly wash them in cold water. Coat them with a VERY light smear of glycerol before you use them, preferably each time. That should help. Bryan Llewellyn. Diane Satterfield via Histonet wrote: > We are using large metal molds to embed mouse brains. We are having a hard time getting to block out of the molds, the paraffin blocks are sticking. Sometimes they are coming out cracked. Sometimes the cassette comes off the paraffin block. Any idea why this is happening? Any advice on how to fix this problem? > > > Diane L. Satterfield, BS > Manager Brain Tumor BioRepository > Research Program Leader > Duke University Medical Center > Brain Tumor Center Biorepository and Database > > diane.satterfield at duke.edu > office 919-684-4642 > pager 919-970-7328 > fax 919-684-4975 > > CONFIDENTIALITY NOTICE: The information contained in this electronic mail is sensitive, protected information intended only for the addressee(s). Any other person, including anyone who believes he/she might have received it due to an addressing error, is requested to notify the sender immediately by return electronic mail, and to delete it without further reading or retention. The information is not to be forwarded to or shared unless in compliance with Duke Medicine policies on confidentiality and/or with the approval of the sender. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 5 Date: Wed, 8 Nov 2017 12:30:06 -0800 From: Bryan Llewellyn To: Diane Satterfield , Histonet Subject: Re: [Histonet] Metal embedding molds-large Message-ID: Content-Type: text/plain; charset=UTF-8; format=flowed Sorry! That should be TSP - trisodium phosphate - not TCP, which might make it worse. Bryan Bryan Llewellyn wrote: > This used to be a common problem years ago. It is due to crud buildup > on the metal. Boil them with TCP for half an hour, then thoroughly > wash them in cold water. Coat them with a VERY light smear of glycerol > before you use them, preferably each time. That should help. > > Bryan Llewellyn. > > Diane Satterfield via Histonet wrote: >> We are using large metal molds to embed mouse brains. We are having >> a hard time getting to block out of the molds, the paraffin blocks >> are sticking. Sometimes they are coming out cracked. Sometimes the >> cassette comes off the paraffin block. Any idea why this is >> happening? Any advice on how to fix this problem? >> >> >> Diane L. Satterfield, BS >> Manager Brain Tumor BioRepository >> Research Program Leader >> Duke University Medical Center >> Brain Tumor Center Biorepository and Database >> >> diane.satterfield at duke.edu >> office 919-684-4642 >> pager 919-970-7328 >> fax 919-684-4975 >> >> CONFIDENTIALITY NOTICE: The information contained in this electronic >> mail is sensitive, protected information intended only for the >> addressee(s). Any other person, including anyone who believes he/she >> might have received it due to an addressing error, is requested to >> notify the sender immediately by return electronic mail, and to >> delete it without further reading or retention. The information is >> not to be forwarded to or shared unless in compliance with Duke >> Medicine policies on confidentiality and/or with the approval of the sender. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > ------------------------------ Message: 6 Date: Wed, 8 Nov 2017 13:46:39 -0800 From: Caroline Miller To: Bryan Llewellyn Cc: Diane Satterfield , Histonet Subject: Re: [Histonet] Metal embedding molds-large Message-ID: Content-Type: text/plain; charset="UTF-8" hey, we do all our embedding in those molds, and here is what I suggest: 1 - Make sure to have enough wax in the back of the molds, all the way until it is on the lip of the cassette - you may need to refill a bunch of times because the wax drains out (why regular sakura cassettes do not fit in these molds, also made by sakura I really don't know). But you need to wait until it hardens enough, but not too much to leave a transition between the two fill waxes. This will prevent the cassette from coming away when you pop it out 2 - Make sure the block is nice and cold, wait 10 minutes longer than you think you have to 3 - Use a spatula or other strong item to place under the label side of the cassette and pop out of the mold, if you get any resistance then WAIT some more! (again them being cold is really important) good luck, happy to answer any clarifying questions! mills On Wed, Nov 8, 2017 at 12:30 PM, Bryan Llewellyn via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Sorry! > > That should be TSP - trisodium phosphate - not TCP, which might make > it worse. > > Bryan > > > > Bryan Llewellyn wrote: > >> This used to be a common problem years ago. It is due to crud buildup >> on the metal. Boil them with TCP for half an hour, then thoroughly >> wash them in cold water. Coat them with a VERY light smear of >> glycerol before you use them, preferably each time. That should help. >> >> Bryan Llewellyn. >> >> Diane Satterfield via Histonet wrote: >> >>> We are using large metal molds to embed mouse brains. We are having >>> a hard time getting to block out of the molds, the paraffin blocks >>> are sticking. Sometimes they are coming out cracked. Sometimes the >>> cassette comes off the paraffin block. Any idea why this is >>> happening? Any advice on how to fix this problem? >>> >>> >>> Diane L. Satterfield, BS >>> Manager Brain Tumor BioRepository >>> Research Program Leader >>> Duke University Medical Center >>> Brain Tumor Center Biorepository and Database >>> >>> diane.satterfield at duke.edu >>> office 919-684-4642 >>> pager 919-970-7328 >>> fax 919-684-4975 >>> >>> CONFIDENTIALITY NOTICE: The information contained in this >>> electronic mail is sensitive, protected information intended only >>> for the addressee(s). Any other person, including anyone who >>> believes he/she might have received it due to an addressing error, >>> is requested to notify the sender immediately by return electronic >>> mail, and to delete it without further reading or retention. The >>> information is not to be forwarded to or shared unless in compliance >>> with Duke Medicine policies on confidentiality and/or with the approval of the sender. >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >> > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 7 Date: Wed, 8 Nov 2017 13:48:13 -0800 From: Caroline Miller To: Terri Braud Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Elastic Stain Message-ID: Content-Type: text/plain; charset="UTF-8" In the UK clinical lab I started in we used Miller's elastin, which I think was a resourcinol formulation (digging from 20 years ago in my brain). I tried to find that in the US but never did. I loved that stain, worked well every time! We would counterstain with a van-Giesen, looked stunning! the elastin came out very dark blue / black. mills On Wed, Nov 8, 2017 at 6:31 AM, Terri Braud via Histonet < histonet at lists.utsouthwestern.edu> wrote: > We love the Aldehyde Fuchsin with a fast-green counterstain. Once the > initial working stain is made up, it is a fast and easy stain to perform, > not to mention just pretty - purple fibers against a green background. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > ************************* > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 8 Date: Thu, 9 Nov 2017 12:23:33 +0000 From: Kathleen S Cormier To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Chrome Alum slides picking up eosin non specifically? Message-ID: <68218F88-E8FA-4D49-82D6-ECAB218B507C at mit.edu> Content-Type: text/plain; charset="us-ascii" Hello Everyone, Was wondering, I am making up some chrome alum slides, and they seem to pick up quite the bit of eosin non specifically. Is this typical? If not, what should I do differently? Thanks! Kathy Kathy Cormier cormier at mit.edu ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 168, Issue 8 **************************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From Kelly.Pairan at nationwidechildrens.org Fri Nov 10 05:39:11 2017 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Fri, 10 Nov 2017 11:39:11 +0000 Subject: [Histonet] EDTA decalcification tissue issues Message-ID: <1510313955091.78906@nationwidechildrens.org> Good Morning, Recently, our lab has been working on validating an EDTA method of decalcification. When we ran the IHC's on the decalcified bone block, the majority of the tissue lifted off the slide. We use the Leica Bonds for our IHC staining. Does anyone else have a hard time getting EDTA decalcified tissue to stay on positively charged slides during IHC runs? Do you have a trick you use? We really did not have this big of a problem when we were decalcifying with Formical or Nitrical (big bones only). Thanks for your help, ?Kelly Pairan, HT(ASCP), QIHC(ASCP) From Richard.Cartun at hhchealth.org Fri Nov 10 11:48:32 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 10 Nov 2017 17:48:32 +0000 Subject: [Histonet] EDTA decalcification tissue issues In-Reply-To: <1510313955091.78906@nationwidechildrens.org> References: <1510313955091.78906@nationwidechildrens.org> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E954799E3@HHCEXCHMB03.hhcsystem.org> We switched from acid decal to EDTA recently; primarily for molecular testing. We are starting to see some excellent results. At first, we were not decaling long enough (EDTA decalcification is a very slow process). We also use the Leica Bond Max and we have not had any problems with tissue loss. We are only using EDTA for small biopsies. Please not that these specimens must be fixed adequately before the decal process is started. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Pairan, Kelly via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, November 10, 2017 6:39 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] EDTA decalcification tissue issues This email is from outside HHC. BE CAREFUL when opening attachments or links from unknown senders. Good Morning, Recently, our lab has been working on validating an EDTA method of decalcification. When we ran the IHC's on the decalcified bone block, the majority of the tissue lifted off the slide. We use the Leica Bonds for our IHC staining. Does anyone else have a hard time getting EDTA decalcified tissue to stay on positively charged slides during IHC runs? Do you have a trick you use? We really did not have this big of a problem when we were decalcifying with Formical or Nitrical (big bones only). Thanks for your help, ?Kelly Pairan, HT(ASCP), QIHC(ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From boznpl at aol.com Fri Nov 10 11:52:22 2017 From: boznpl at aol.com (Laurie Colbert) Date: Fri, 10 Nov 2017 12:52:22 -0500 Subject: [Histonet] Part time IHC position in LA area Message-ID: <15fa70fa01c-c0d-15d69@webjas-vab193.srv.aolmail.net> I am posting this for a friend of mine who was recently laid off.? He is a PhD, not a histotech, and has worked doing IHC for over 30 years.? He is very experienced and competent.? He is looking for a part time IHC position in the LA area. Please see all of his contact info below. Sabir Husain 909-224-9866 sabirhusainihc at hotmail.com From bhartologist at gmail.com Sun Nov 12 21:41:03 2017 From: bhartologist at gmail.com (Bharti Parihar) Date: Sun, 12 Nov 2017 19:41:03 -0800 Subject: [Histonet] The Use of Plants in Histology Laboratories Message-ID: Hello all! Are there any labs out there using plants in their processing rooms to reduce formaldehyde and xylene fumes? If so, please share how you went about this and if there have been issues with contamination? -- Bharti Parihar, HT (ASCP)CM From abtdhu at gmail.com Mon Nov 13 08:52:21 2017 From: abtdhu at gmail.com (Dorothy Hu) Date: Mon, 13 Nov 2017 09:52:21 -0500 Subject: [Histonet] EDTA decalcification tissue issues Message-ID: We didn't compare EDTA with formic acid. But heard that formic acid also can do many IHC and ISH, if not all the antibodies and all probes. I always have problem of bone falling off, no matter what kind of slides. Thinking both PFA and EDTA affect on this issue. So trying frozen tape unfixed, undecalcified bone now. If anyone has research paper regarding this, please share. Thanks. Dorothy Hu From rsrichmond at gmail.com Mon Nov 13 10:18:43 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Mon, 13 Nov 2017 11:18:43 -0500 Subject: [Histonet] Obituary - pathologist Bernard Leon Klionsky Message-ID: With his permission I post pathologist Leon Metlay's Facebook obituary of his wife's father: My father-in-law, Dr. Bernard Leon Klionsky, died [November 11th, 2017] at the age of 92. He was one of the unsung heroes of pathology. As a young man, he invented the form of cryostat that we all use to this day, with the microtome down inside the freezer. He was one of the greatest teachers I've ever known. He was primarily known as a cytopathologist, but was also an early member of the Pediatric Pathology Club. As a child, during the Depression, he sold ice cream from a box on his bicycle. He realized that the ice cream stayed cold with the opening on the top. He applied that to cryostats. The paper was (I think) in AJCP. Some time in the late '50s. I asked him: What was his relationship to International / Damon / IEC (I don't remember the exact name), that as far as I know brought out the first practical refrigerated microtomes? I think they came along in 1960, and were in common use by the time I got into pathology in 1964, though the old "wet knife" method continued in common use into the 1970s. He replied: He had no relationship with any manufacturer as far as I know. As I heard it, he tried to put the idea in the public domain, so that cryostats would be less expensive. I don't know what happened. The University of Kansas got a free cryostat while he was there. When he moved on to Pitt, the manufacturer took back the cryostat. Does any of our old-timers remember any of this? Bob Richmond Samurai Pathologist Maryville TN From Beth_Mickley at URMC.Rochester.edu Mon Nov 13 14:24:31 2017 From: Beth_Mickley at URMC.Rochester.edu (Mickley, Beth) Date: Mon, 13 Nov 2017 20:24:31 +0000 Subject: [Histonet] The Use of Plants in Histology Laboratories In-Reply-To: References: Message-ID: <1510604671172.29915@URMC.Rochester.edu> I found this great article about plants used in laboratories: Plants That Can Clean Up Your Indoor Air Plants clean indoor air in two ways?by absorbing contaminants through pores on the leaves, and by metabolizing contaminants through organisms living in the soil. In fact, plants are so effective that some stores, like Lowe?s and Home Depot, are starting to label the most effective ones with tags. Though it seems most plants will benefit indoor air, the following are those that have been shown in scientific studies and shown to work. These plants can also help maintain humidity levels and remove mold spores and bacteria from the air. 1.Spider Plant: formaldehyde, xylene and toluene. 2.Golden Pothos: benzene, formaldehyde, trichloroethylene, xylene and toluene. 3.Snake Plant (Mother-in-Law?s Tongue): benzene, formaldehyde, trichloroethylene, xylene and toluene. 4.Bamboo Palm or Reed Palm: formaldehyde, xylene, and toluene. 5.Chinese Evergreen: benzene, formaldehyde. 6.Peace Lily: benzene, formaldehyde, trichloroethylene, xylene, toluene, and ammonia. 7.English Ivy: mold and mildew, formaldehyde, benzene, xylene, and toluene. 8.Gerbera Daisies: benzene, formaldehyde, trichloroethylene. 9.Red-Edged Dracaena (Dracaena Marginata): benzene, formaldehyde, trichloroethylene, xylene, and toluene. 10.Warneck Dracaena: benzene, trichloroethylene, xylene, and toluene. 11.Weeping Fig: formaldehyde, xylene, and toluene. 12.Chrysanthemum: formaldehyde, benzene, trichloroethylene, xylene, toluene, and ammonia. 13.Boston fern: formaldehyde, xylene and toluene. 14.Philodendron: formaldehyde. Beth Geer, HT Mohs Surgery University Dermatology Associates Rochester, NY ________________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Monday, November 13, 2017 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 168, Issue 10 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=k8wthWMVkqXuqUqqzZHV30GZnwgu7td9mpPmY3_7vBk&m=3j2a9oNdNzfMT-HS8S1P-6g49hennezEvpfXg6SM2B8&s=TcVH5qahDDnUM0zmI0Mzv1GfaZLh-hKDifP2KHwf1J8&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. The Use of Plants in Histology Laboratories (Bharti Parihar) 2. EDTA decalcification tissue issues (Dorothy Hu) 3. Obituary - pathologist Bernard Leon Klionsky (Bob Richmond) ---------------------------------------------------------------------- Message: 1 Date: Sun, 12 Nov 2017 19:41:03 -0800 From: Bharti Parihar To: Histonet Archive Subject: [Histonet] The Use of Plants in Histology Laboratories Message-ID: Content-Type: text/plain; charset="UTF-8" Hello all! Are there any labs out there using plants in their processing rooms to reduce formaldehyde and xylene fumes? If so, please share how you went about this and if there have been issues with contamination? -- Bharti Parihar, HT (ASCP)CM ------------------------------ Message: 2 Date: Mon, 13 Nov 2017 09:52:21 -0500 From: Dorothy Hu To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] EDTA decalcification tissue issues Message-ID: Content-Type: text/plain; charset="UTF-8" We didn't compare EDTA with formic acid. But heard that formic acid also can do many IHC and ISH, if not all the antibodies and all probes. I always have problem of bone falling off, no matter what kind of slides. Thinking both PFA and EDTA affect on this issue. So trying frozen tape unfixed, undecalcified bone now. If anyone has research paper regarding this, please share. Thanks. Dorothy Hu ------------------------------ Message: 3 Date: Mon, 13 Nov 2017 11:18:43 -0500 From: Bob Richmond To: "Histonet at lists.utsouthwestern.edu" Subject: [Histonet] Obituary - pathologist Bernard Leon Klionsky Message-ID: Content-Type: text/plain; charset="UTF-8" With his permission I post pathologist Leon Metlay's Facebook obituary of his wife's father: My father-in-law, Dr. Bernard Leon Klionsky, died [November 11th, 2017] at the age of 92. He was one of the unsung heroes of pathology. As a young man, he invented the form of cryostat that we all use to this day, with the microtome down inside the freezer. He was one of the greatest teachers I've ever known. He was primarily known as a cytopathologist, but was also an early member of the Pediatric Pathology Club. As a child, during the Depression, he sold ice cream from a box on his bicycle. He realized that the ice cream stayed cold with the opening on the top. He applied that to cryostats. The paper was (I think) in AJCP. Some time in the late '50s. I asked him: What was his relationship to International / Damon / IEC (I don't remember the exact name), that as far as I know brought out the first practical refrigerated microtomes? I think they came along in 1960, and were in common use by the time I got into pathology in 1964, though the old "wet knife" method continued in common use into the 1970s. He replied: He had no relationship with any manufacturer as far as I know. As I heard it, he tried to put the idea in the public domain, so that cryostats would be less expensive. I don't know what happened. The University of Kansas got a free cryostat while he was there. When he moved on to Pitt, the manufacturer took back the cryostat. Does any of our old-timers remember any of this? Bob Richmond Samurai Pathologist Maryville TN ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=k8wthWMVkqXuqUqqzZHV30GZnwgu7td9mpPmY3_7vBk&m=3j2a9oNdNzfMT-HS8S1P-6g49hennezEvpfXg6SM2B8&s=TcVH5qahDDnUM0zmI0Mzv1GfaZLh-hKDifP2KHwf1J8&e= ------------------------------ End of Histonet Digest, Vol 168, Issue 10 ***************************************** From regan.fulton at gmail.com Mon Nov 13 16:38:57 2017 From: regan.fulton at gmail.com (Fulton Regan) Date: Mon, 13 Nov 2017 14:38:57 -0800 Subject: [Histonet] validation Message-ID: <578940D8-C935-457F-8327-EADA7AC061EF@gmail.com> Hello Nancy, Given your interest in best practices for validations, I?d like to offer some recent literature references that may be of some use. Your use of 20 positives and 20 negatives exceeds the minimum standard, from CAP Guidance. For purely diagnostic markers. (10+ and 10- are suggested in such cases), while 20+ and 20- are suggested as a minimum for predictive markers, such as ER, PR, etc? Your practice exceeds the minimum, but is certainly an excellent approach for better control of your assays! Patrick, L. F., Linda, A. B., Lisa, A. F., Alsabeh, R., Regan, S. F., Jeffrey, D. G., ? Swanson, P. E. (2014). Principles of analytic validation of immunohistochemical assays: Guideline from the College of American Pathologists Pathology and Laboratory Quality Center. Archives of Pathology and Laboratory Medicine, 138(11), 1432?1443. https://doi.org/10.5858/arpa.2013-0610-CP With respect to negative controls, there is another good reference, below: Torlakovic, E. E., Francis, G., Garratt, J., Gilks, B., Hyjek, E., Ibrahim, M., ? Vyberg, M. (2014). Standardization of Negative Controls in Diagnostic Immunohistochemistry. Applied Immunohistochemistry & Molecular Morphology, 22(4), 241?252. https://doi.org/10.1097/PAI.0000000000000069 Internal negative control elements can definitely be part of the validation plan and can be used in specificity calculations. Of course, it is important to confirm that these internal negatives are in fact negative! However, it is also best practice to design validations as ?fit for purpose?. That is, does the assay reliably distinguish among the differential diagnostic considerations? So, for specificity, a collection of ?pertinent negatives? should also be included in the validation. As an example: in the case of Beta-catenin for the diagnosis of fibromatosis, one should include some cases that resemble fibromatosis. These might include, GIST, SCHWANNOMA, LEIOMYOMA, NEUROFIBROMA, and others, as your question clearly anticipated. I hope this of some use to you. Feel free to contact me if I can be of any further help. Regan Regan Fulton, MD/PhD CEO Array Science, LLC Tissue Microarray Technologies 475 Gate 5 Road, #102 Sausalito, CA 94965 fulton at ArrayScience.com From allanw at biomax.us Mon Nov 13 16:46:45 2017 From: allanw at biomax.us (Allan Wang) Date: Mon, 13 Nov 2017 17:46:45 -0500 Subject: [Histonet] mounting medium In-Reply-To: References: Message-ID: Hi, This information would be very useful for me and probably others as well, since I've just arbitrarily chosen one. Can you summarize the responses you received for us? Allan On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello to all > > Can anyone suggest which mouting medium is best that will help preservation > of staining properties without creating fading of stains and bubbles on > long run. > Thanks alot > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From laurie.reilly at jcu.edu.au Mon Nov 13 17:35:01 2017 From: laurie.reilly at jcu.edu.au (Reilly, Laurie) Date: Mon, 13 Nov 2017 23:35:01 +0000 Subject: [Histonet] mounting medium In-Reply-To: References: Message-ID: We also would be interested in other's thoughts on mounting media. We are having coverslips coming unstuck from some of our teaching slides after 3 or 4 years. It is very frustrating when cover slipping manually and wondering how long it will last. Thanks and regards, Laurie. Mr. Laurie REILLY Histopathology Veterinary and Biomedical Sciences James Cook University Townsville? Qld.? 4811 Australia. Phone 07 4781 4468 Mobile 0448 957747 -----Original Message----- From: Allan Wang via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, 14 November 2017 8:47 AM To: warda hassan Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] mounting medium Hi, This information would be very useful for me and probably others as well, since I've just arbitrarily chosen one. Can you summarize the responses you received for us? Allan On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello to all > > Can anyone suggest which mouting medium is best that will help > preservation of staining properties without creating fading of stains > and bubbles on long run. > Thanks alot > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Mon Nov 13 17:52:46 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 13 Nov 2017 23:52:46 +0000 Subject: [Histonet] mounting medium In-Reply-To: References: Message-ID: We use Permaslip acrylic for manual coverslipping. Sakura media for our automated coverslipper. We have also used Richard-Allan CytoSeal with great resuls. We stopped using one called Quick-mount (comes in tube) because they had a batch that was bad and the media turned cloudy and the slips dropped off after about 6 months. That has been a pain.... Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Reilly, Laurie via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, November 13, 2017 3:35 PM To: Allan Wang; warda hassan; Histonet at lists.utsouthwestern.edu Cc: Hautaniemi, Walter; Reilly, Sue; Reeks, Karen Subject: Re: [Histonet] mounting medium We also would be interested in other's thoughts on mounting media. We are having coverslips coming unstuck from some of our teaching slides after 3 or 4 years. It is very frustrating when cover slipping manually and wondering how long it will last. Thanks and regards, Laurie. Mr. Laurie REILLY Histopathology Veterinary and Biomedical Sciences James Cook University Townsville? Qld.? 4811 Australia. Phone 07 4781 4468 Mobile 0448 957747 -----Original Message----- From: Allan Wang via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, 14 November 2017 8:47 AM To: warda hassan Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] mounting medium Hi, This information would be very useful for me and probably others as well, since I've just arbitrarily chosen one. Can you summarize the responses you received for us? Allan On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello to all > > Can anyone suggest which mouting medium is best that will help > preservation of staining properties without creating fading of stains > and bubbles on long run. > Thanks alot > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stefano.Mantero at humanitasresearch.it Tue Nov 14 03:38:26 2017 From: Stefano.Mantero at humanitasresearch.it (MANTERO Stefano RIC) Date: Tue, 14 Nov 2017 09:38:26 +0000 Subject: [Histonet] R: EDTA decalcification tissue issues In-Reply-To: <1510313955091.78906@nationwidechildrens.org> References: <1510313955091.78906@nationwidechildrens.org> Message-ID: <43d57559b277441a8ca970b50e6e0518@exmbx01.techosp.it> Good Morning Kelly, I work in a research institute that works with murine samples; In our laboratory we have adopted two descaling protocols: one with ion exchange resins/acid solution while the other uses EDTA. The EDTA descaling system is slower but keeps the nucleic acids much better. We use an 14% solution of tetrasodium EDTA in water buffered to pH 7.4-7.6 with acetic acid, the solution is left for several days (from 5 to 10 depending on the density and size of the bone) with daily solution changes. Have a good work Stefano Dott. Stefano Mantero Human Genome Laboratory CNR-IRGB c/o Humanitas Research Hospital Via Rita Levi Montalcini (Ex Via Dainese) 20090 Pieve Emanuele (MI) Ph. +39 02 8224 5164 (desk) Ph. +39 02 8224 5177 (lab) Fax +39 02 8224 5191 ------------------------------------------ DAI IL TUO 5x1000 ALLA RICERCA HUMANITAS. Codice fiscale 10125410158 (Ricerca sanitaria) http://www.humanitas.it/5x1000 ------------------------------------------ Nota di riservatezza. Il presente messaggio, corredato dei relativi allegati, contiene informazioni da considerarsi strettamente riservate, ed ? destinato esclusivamente al destinatario sopra indicato, il quale ? l'unico autorizzato ad usarlo, copiarlo e, sotto la propria responsabilit?, diffonderlo. Chiunque ricevesse questo messaggio per errore o comunque lo leggesse senza esserne legittimato ? avvertito che trattenerlo, copiarlo, divulgarlo, distribuirlo a persone diverse dal destinatario ? severamente proibito, ed ? pregato di rinviarlo immediatamente al mittente distruggendone l'originale. Grazie Confidentiality Notice. This message, together with its annexes, contains information to be deemed strictly confidential and is destined only to the addressee(s) identified above who only may use, copy and, under his/their responsibility, further disseminate it. If anyone received this message by mistake or reads it without entitlement is forewarned that keeping, copying, disseminating or distributing this message to persons other than the addressee(s) is strictly forbidden and is asked to transmit it immediately to the sender and to erase the original message received. Thank You -----Messaggio originale----- Da: Pairan, Kelly via Histonet [mailto:histonet at lists.utsouthwestern.edu] Inviato: venerd? 10 novembre 2017 12:39 A: histonet at lists.utsouthwestern.edu Oggetto: [Histonet] EDTA decalcification tissue issues Good Morning, Recently, our lab has been working on validating an EDTA method of decalcification. When we ran the IHC's on the decalcified bone block, the majority of the tissue lifted off the slide. We use the Leica Bonds for our IHC staining. Does anyone else have a hard time getting EDTA decalcified tissue to stay on positively charged slides during IHC runs? Do you have a trick you use? We really did not have this big of a problem when we were decalcifying with Formical or Nitrical (big bones only). Thanks for your help, ?Kelly Pairan, HT(ASCP), QIHC(ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SJMcCabe at phhealthcare.org Tue Nov 14 07:03:00 2017 From: SJMcCabe at phhealthcare.org (McCabe, Sara) Date: Tue, 14 Nov 2017 13:03:00 +0000 Subject: [Histonet] HSV I/II Control Message-ID: <1510664580589.25809@phhealthcare.org> Awhile ago I had posted looking for anyone that had any in house HSV controls. A gentleman had reached out to me; however, I never did receive the controls that he said he was sending me. I am trying to reach him. I am also looking for anyone else that may also have HSV controls. We are in need. I would rather not purchase commercial control slides. Thank you in advance for your help! Sara J. McCabe, HT(ASCP)CM Histology Supervisor Penn Highlands DuBois 100 Hospital Avenue DuBois, PA 15801 814-375-7514 Telephone-Office 814-375-3264 Telephone-Histology Lab 814-375-3784 Fax sjmccabe at phhealthcare.org www.phhealthcare.org [PHH ESig Logo 150dpi-01] From diane.satterfield at duke.edu Tue Nov 14 08:29:49 2017 From: diane.satterfield at duke.edu (Diane Satterfield) Date: Tue, 14 Nov 2017 14:29:49 +0000 Subject: [Histonet] mounting medium In-Reply-To: References: Message-ID: We use Sub-X Mounting Medium from Leica. This is what the pathologist wants us to use. -----Original Message----- From: Allan Wang via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, November 13, 2017 5:47 PM To: warda hassan Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] mounting medium Hi, This information would be very useful for me and probably others as well, since I've just arbitrarily chosen one. Can you summarize the responses you received for us? Allan On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello to all > > Can anyone suggest which mouting medium is best that will help preservation > of staining properties without creating fading of stains and bubbles on > long run. > Thanks alot > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=imBPVzF25OnBgGmVOlcsiEgHoG1i6YHLR0Sj_gZ4adc&r=eLj2B87Mkll0NUAmalTD_1fRsqzhM-r-54bMn0ykFbs&m=qAeXtP7MVtiB5tSOrrA22EAPWyiHIuG7ijlXdENpf68&s=-1VDk505lt6WIaFntwlARL_RqLOgFH02KVXBwp7kovE&e= > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=imBPVzF25OnBgGmVOlcsiEgHoG1i6YHLR0Sj_gZ4adc&r=eLj2B87Mkll0NUAmalTD_1fRsqzhM-r-54bMn0ykFbs&m=qAeXtP7MVtiB5tSOrrA22EAPWyiHIuG7ijlXdENpf68&s=-1VDk505lt6WIaFntwlARL_RqLOgFH02KVXBwp7kovE&e= From mlm11 at cornell.edu Tue Nov 14 08:51:09 2017 From: mlm11 at cornell.edu (Mary Lou Norman) Date: Tue, 14 Nov 2017 14:51:09 +0000 Subject: [Histonet] mounting medium In-Reply-To: References: Message-ID: Refrax from Anatech -----Original Message----- From: Allan Wang via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, November 13, 2017 5:47 PM To: warda hassan Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] mounting medium Hi, This information would be very useful for me and probably others as well, since I've just arbitrarily chosen one. Can you summarize the responses you received for us? Allan On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello to all > > Can anyone suggest which mouting medium is best that will help > preservation of staining properties without creating fading of stains > and bubbles on long run. > Thanks alot > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Tue Nov 14 12:34:23 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 14 Nov 2017 18:34:23 +0000 Subject: [Histonet] Mounting media Message-ID: <48E053DDF6CE074DB6A7414BA05403F84CE9DE70@HRHEX03-HOS.holyredeemer.local> Although we've used several brands with good success, our most consistent performer has been the Sakura Tissue Tek Glas Mounting Media #6419. When coverslipping, either automated or manual, the secret to avoiding air bubble during storage is to insure that the correct amount of media is dispensed onto the slide. If the amount is insufficient, the slide will still coverslip to be read, but as time passes and the xylene dries out, there will be air left under the coverglass which will allow the stain to degrade. When coverslipping by hand, we go by the rule of 3 drops of media from a plastic disposable pipette for a 24x50 No.1 coverglass. When techs "guesstimate" is when problems occur. Best of luck, I hope this helps Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From jshelley at sbpdiscovery.org Tue Nov 14 13:06:26 2017 From: jshelley at sbpdiscovery.org (John Shelley) Date: Tue, 14 Nov 2017 19:06:26 +0000 Subject: [Histonet] 2018 FSH Annual Meeting Message-ID: Hello Histonetters! The FSH Annual Meeting will be in Tampa, FL on May 17-20, 2018 at the Renaissance Tampa International Plaza Hotel. We are looking for ideas for classes that you will be interested in attending. We are also looking for speakers. Send abstracts to email below. We want to hear from you. Contact us at fshgrouppresidentgmail.com See you in Tampa. Kind Regards! John J Shelley fshgrouppresident at gmail.com From TNMayer at mdanderson.org Tue Nov 14 15:10:33 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Tue, 14 Nov 2017 21:10:33 +0000 Subject: [Histonet] The Use of Plants in Histology Laboratories (Mickley, Beth) Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC883A065046@D1PWPEXMBX08.mdanderson.edu> Beth, We sure could have used the actual article in a lab I know of. The person with the highest authority, removed them from a lab, and did not want to listen to what the supervisor had to say. Without the actual article, nothing could change her mind. It is common to have spider plants, and ivy in labs to help with the air quality. Now the EHS departments need to know about it as well. Toysha Message: 1 Date: Mon, 13 Nov 2017 20:24:31 +0000 From: "Mickley, Beth" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] The Use of Plants in Histology Laboratories Message-ID: <1510604671172.29915 at URMC.Rochester.edu> Content-Type: text/plain; charset="Windows-1252" I found this great article about plants used in laboratories: Plants That Can Clean Up Your Indoor Air Plants clean indoor air in two ways?by absorbing contaminants through pores on the leaves, and by metabolizing contaminants through organisms living in the soil. In fact, plants are so effective that some stores, like Lowe?s and Home Depot, are starting to label the most effective ones with tags. Though it seems most plants will benefit indoor air, the following are those that have been shown in scientific studies and shown to work. These plants can also help maintain humidity levels and remove mold spores and bacteria from the air. 1.Spider Plant: formaldehyde, xylene and toluene. 2.Golden Pothos: benzene, formaldehyde, trichloroethylene, xylene and toluene. 3.Snake Plant (Mother-in-Law?s Tongue): benzene, formaldehyde, trichloroethylene, xylene and toluene. 4.Bamboo Palm or Reed Palm: formaldehyde, xylene, and toluene. 5.Chinese Evergreen: benzene, formaldehyde. 6.Peace Lily: benzene, formaldehyde, trichloroethylene, xylene, toluene, and ammonia. 7.English Ivy: mold and mildew, formaldehyde, benzene, xylene, and toluene. 8.Gerbera Daisies: benzene, formaldehyde, trichloroethylene. 9.Red-Edged Dracaena (Dracaena Marginata): benzene, formaldehyde, trichloroethylene, xylene, and toluene. 10.Warneck Dracaena: benzene, trichloroethylene, xylene, and toluene. 11.Weeping Fig: formaldehyde, xylene, and toluene. 12.Chrysanthemum: formaldehyde, benzene, trichloroethylene, xylene, toluene, and ammonia. 13.Boston fern: formaldehyde, xylene and toluene. 14.Philodendron: formaldehyde. Beth Geer, HT Mohs Surgery University Dermatology Associates Rochester, NY ________________________________________ The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From Julia.Cates at AHSS.ORG Wed Nov 15 14:17:41 2017 From: Julia.Cates at AHSS.ORG (Cates, Julia) Date: Wed, 15 Nov 2017 20:17:41 +0000 Subject: [Histonet] The Use of Plants in Histology Laboratories Message-ID: I think from a safety and infection control perspective, house plants, while beneficial for air quality, cannot be cleaned or disinfected. An environment of care committee or safety officer would veto the plants based on the that. Thanks, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Park Ridge Health (828)650-8243| Fax: (828)209-5315 Confidentiality Statement: This email message, including any attachments, is for the sole?use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited.? If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Wednesday, November 15, 2017 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 168, Issue 12 ** WARNING: This email originated outside of AHS. ** DO NOT CLICK links or attachments unless you recognize the sender and know the content is safe. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Mounting media (Terri Braud) 2. 2018 FSH Annual Meeting (John Shelley) 3. Re: The Use of Plants in Histology Laboratories (Mickley, Beth) (Mayer,Toysha N) ---------------------------------------------------------------------- Message: 1 Date: Tue, 14 Nov 2017 18:34:23 +0000 From: "Terri Braud" To: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] Mounting media Message-ID: <48E053DDF6CE074DB6A7414BA05403F84CE9DE70 at HRHEX03-HOS.holyredeemer.local> Content-Type: text/plain; charset="us-ascii" Although we've used several brands with good success, our most consistent performer has been the Sakura Tissue Tek Glas Mounting Media #6419. When coverslipping, either automated or manual, the secret to avoiding air bubble during storage is to insure that the correct amount of media is dispensed onto the slide. If the amount is insufficient, the slide will still coverslip to be read, but as time passes and the xylene dries out, there will be air left under the coverglass which will allow the stain to degrade. When coverslipping by hand, we go by the rule of 3 drops of media from a plastic disposable pipette for a 24x50 No.1 coverglass. When techs "guesstimate" is when problems occur. Best of luck, I hope this helps Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal ------------------------------ Message: 2 Date: Tue, 14 Nov 2017 19:06:26 +0000 From: John Shelley To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] 2018 FSH Annual Meeting Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Histonetters! The FSH Annual Meeting will be in Tampa, FL on May 17-20, 2018 at the Renaissance Tampa International Plaza Hotel. We are looking for ideas for classes that you will be interested in attending. We are also looking for speakers. Send abstracts to email below. We want to hear from you. Contact us at fshgrouppresidentgmail.com See you in Tampa. Kind Regards! John J Shelley fshgrouppresident at gmail.com ------------------------------ Message: 3 Date: Tue, 14 Nov 2017 21:10:33 +0000 From: "Mayer,Toysha N" To: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] The Use of Plants in Histology Laboratories (Mickley, Beth) Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC883A065046 at D1PWPEXMBX08.mdanderson.edu> Content-Type: text/plain; charset="us-ascii" Beth, We sure could have used the actual article in a lab I know of. The person with the highest authority, removed them from a lab, and did not want to listen to what the supervisor had to say. Without the actual article, nothing could change her mind. It is common to have spider plants, and ivy in labs to help with the air quality. Now the EHS departments need to know about it as well. Toysha Message: 1 Date: Mon, 13 Nov 2017 20:24:31 +0000 From: "Mickley, Beth" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] The Use of Plants in Histology Laboratories Message-ID: <1510604671172.29915 at URMC.Rochester.edu> Content-Type: text/plain; charset="Windows-1252" I found this great article about plants used in laboratories: Plants That Can Clean Up Your Indoor Air Plants clean indoor air in two ways?by absorbing contaminants through pores on the leaves, and by metabolizing contaminants through organisms living in the soil. In fact, plants are so effective that some stores, like Lowe?s and Home Depot, are starting to label the most effective ones with tags. Though it seems most plants will benefit indoor air, the following are those that have been shown in scientific studies and shown to work. These plants can also help maintain humidity levels and remove mold spores and bacteria from the air. 1.Spider Plant: formaldehyde, xylene and toluene. 2.Golden Pothos: benzene, formaldehyde, trichloroethylene, xylene and toluene. 3.Snake Plant (Mother-in-Law?s Tongue): benzene, formaldehyde, trichloroethylene, xylene and toluene. 4.Bamboo Palm or Reed Palm: formaldehyde, xylene, and toluene. 5.Chinese Evergreen: benzene, formaldehyde. 6.Peace Lily: benzene, formaldehyde, trichloroethylene, xylene, toluene, and ammonia. 7.English Ivy: mold and mildew, formaldehyde, benzene, xylene, and toluene. 8.Gerbera Daisies: benzene, formaldehyde, trichloroethylene. 9.Red-Edged Dracaena (Dracaena Marginata): benzene, formaldehyde, trichloroethylene, xylene, and toluene. 10.Warneck Dracaena: benzene, trichloroethylene, xylene, and toluene. 11.Weeping Fig: formaldehyde, xylene, and toluene. 12.Chrysanthemum: formaldehyde, benzene, trichloroethylene, xylene, toluene, and ammonia. 13.Boston fern: formaldehyde, xylene and toluene. 14.Philodendron: formaldehyde. Beth Geer, HT Mohs Surgery University Dermatology Associates Rochester, NY ________________________________________ The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 168, Issue 12 ***************************************** This message (including any attachments) is intended only for the use of the individual or entity to which it is addressed and may contain information that is non-public, proprietary, privileged, confidential, and exempt from disclosure under applicable law or may constitute as attorney work product. If you are not the intended recipient, you are hereby notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, notify us immediately by telephone and (i) destroy this message if a facsimile or (ii) delete this message immediately if this is an electronic communication. Thank you. From tkngflght at yahoo.com Wed Nov 15 17:20:46 2017 From: tkngflght at yahoo.com (Cheryl) Date: Wed, 15 Nov 2017 23:20:46 +0000 (UTC) Subject: [Histonet] Lead/Supervisor Histo position in Houston References: <1155226606.739610.1510788046477.ref@mail.yahoo.com> Message-ID: <1155226606.739610.1510788046477@mail.yahoo.com> Hi Guys- We are still looking for the right fit for my skin/gi reference lab in Houston.?? Recently moved to newly built facilities in the south end.? BeAuTiFuL!!? Really functional. We're about to step into our next development and need a solid knowlegable team player to support the lab through the next growth phase.? It's a FUN crew, our owner shows his love through SNACKS, frequent lunches, an occasional happy hour outing and an amazing specialty coffee machine of our very own in the lunch room.? We have fun through the day and the work is top notch.?? With such a lovely work environment we're taking care to hire someone who fits the culture AND has an old-school work ethic.? ?Shoot me your resume, give me a call.? Must be supervisory eligible and CLIA '88 gross eligible - ?Cheryl Kerry, HT(ASCP), Operations Mgr.?ADG Houston Pathology ckerry at adgpath.com281.661.1825 Main Phone From Melissa.Kuhnla at chsli.org Thu Nov 16 09:21:55 2017 From: Melissa.Kuhnla at chsli.org (Kuhnla, Melissa) Date: Thu, 16 Nov 2017 15:21:55 +0000 Subject: [Histonet] PDL-1 Message-ID: <4F2CE306C672504AAE5243945B1B4CD718E9314F@MVDCVM01XCN004.chsli.org> Good Morning, Has anyone purchased PDL-1 (22C3 from DAKO) as a concentrated antibody and validated on a Ventana Ultra? Any success? Thank you Melissa Kuhnla Lead Medical Technologist for IHC and FISH testing Regional Laboratory Services Good Samaritan Hospital 631-609-2551 The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From vanessa.keeton03 at gmail.com Thu Nov 16 12:11:38 2017 From: vanessa.keeton03 at gmail.com (Vanessa Keeton) Date: Thu, 16 Nov 2017 13:11:38 -0500 Subject: [Histonet] Validating Cytospin Special Stains Message-ID: Would anyone be willing to share how to go about validating special stains on cytospins? Could you use past cases where you performed a GMS or AFB on both cytospins and cell blocks and if the results correlated then document? Would 10 cases be enough? Any help would be greatly appreciated. Vanessa Keeton From Karen.Heckford at DignityHealth.org Fri Nov 17 11:27:34 2017 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Fri, 17 Nov 2017 17:27:34 +0000 Subject: [Histonet] Non gyn cytospins Message-ID: <46d672056bab469598dfa3da860dfbd3@PHX-EXCH-013.chw.edu> Good Morning, Is there a difference between the time screening Non-gyn cytospins vs. Gyn? If so where can I find the publication to back this up? Just wondering. We got inspected and this came up because our Pathologists screen and read out the Non gyn cytospins. We do not do Gyn's here. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you From shenker at fit.edu Fri Nov 17 12:44:54 2017 From: shenker at fit.edu (Jonathan Shenker) Date: Fri, 17 Nov 2017 18:44:54 +0000 Subject: [Histonet] histology of larval fish olfactory apparatus Message-ID: One of my graduate students is attempting to characterize the ontogeny of the olfactory apparatus of larval fishes, specifically the leptocephalus ("slender head") larvae of tarpon/bonefish/eels, and to develop a comparison with olfactory morphology in larvae of other fishes. She's interested in using fluorescent staining with confocal microscopy of whole larvae and histological sectioning of the olfactory apparatus and nerve, as well as employing more traditional histological staining techniques to visualize the olfactory pits, sensors, and neurons that innervate the organ. She'll also use SEM for high-resolution imagery of the physical structures on the surface of the heads of the larvae. My lab is pretty good with fish biology and ecology, but we're novices when it comes to histological analysis of tissues. As we got into the literature on fluorescent, visual, and histological stains of neurons in general, we quickly got overwhelmed with variety of stains and techniques that have been used, but the literature seems pretty sparse on olfactory apparatus of fish larvae. If Histonet folks have any advice on stains and staining protocols that she can use to visualize the components of the olfactory and nervous system of these transparent larvae, we'd be very appreciative of the guidance. A quick guide to Leptocephali: these larvae are beautiful, transparent laterally-compressed ribbons, clear as glass, with tiny heads. They typically hatch into 3-4 mm long larvae, and grow to different sizes (tarpon = about 25 mm; bonefish about 50 mm; an unknown eel species has a larva that reaches nearly a meter in length). Why are we focusing on leptocephalus olfaction? Lepto olfactory pits are about as big as their eyes; their early retinas are rod-based w/out cones, so they can't see plankton particles (most higher teleost larvae have densely-packed cones in their retinas that help them visualize planktonic prey, and they have small olfactory pits); leptos have long projecting teeth (fangs) that suggest they "carve" chunks out of mucous aggregates called "marine snow" and gelatinous plankton for food; and the stable isotope signature of their bodies closely mirrors that of marine snow. How do they find their food? We're betting on olfaction/chemosensory cues, but we'd like to confirm that hypothesis with morphological and histological characterization of their olfactory apparatus, and hopefully combine it with behavioral analysis of live larvae (if we can get them). It'll make a very interesting study. Any suggestions about how to visualize their olfactory apparatus will be greatly appreciated! Jon Jon Shenker, Ph.D. Associate Professor of Marine Biology Department of Biological Sciences Florida Institute of Technology 150 West University Boulevard Melbourne, FL 32904 321-674-8145 ------------------------------------------------------------------------------------------------------------------------- "...a constant stream of eager young folk, men and women, come to me with the same query. 'My present work is dull. How do I become an ichthyologist?'" J.L.B. Smith, 1956 "The Search Beneath The Sea." ------------------------------------------------------------------------------------------------------------------------- From nelsonrnch at verizon.net Fri Nov 17 15:09:02 2017 From: nelsonrnch at verizon.net (Patti Nelson - PN Lab Consultant) Date: Fri, 17 Nov 2017 16:09:02 -0500 Subject: [Histonet] Billing for Independent Lab Message-ID: <15fcbd03a32-c0a-10754@webjas-vae185.srv.aolmail.net> Hi Histo Land, Is there anyone willing to share their wisdom with me regarding Billing for an independent lab. If so, please contact me at the phone number below. Sincerely, PATTI NELSON H.T.(ASCP) PN LABORATORY CONSULTANTS SUPERVISOR DGC/ZADEH LABS PO BOX 412 CABAZON, CA. 92230 909-841-9761 nelsonrnch at verizon.net CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender ofthe delivery error by e-mail or you may call 909-841-9761. From bakevictoria at gmail.com Fri Nov 17 21:32:27 2017 From: bakevictoria at gmail.com (Victoria Baker) Date: Fri, 17 Nov 2017 22:32:27 -0500 Subject: [Histonet] Epic and Cerner users, looking for feedback on these systems In-Reply-To: References: Message-ID: Happy Friday! Our hospital is starting to look at new HIS vendors. The two that are being strongly looked at are Cerner and Epic. Of course the hospital is using a consulting firm who have sort of tied our hands in terms of following up with vendors and any after the demo questions we are to filter through them. We have been told though, by one of the hospital directors that we can reach out to colleagues and questions. Currently we have SoftPath DX. The system is labor intensive and the version we have is not in keeping with our needs. But it has taken us further than we were before in terms of being able to track cases and monitor our workflow. We are paired in the hospital with several different systems such as NexGen and Sorian for financials. There is also Oracle database which causes some headaches as well. I would like to get an idea of what people think that use either of these systems. Do they slow down work flow? Is it generally user friendly? Can you track all entries and produce accurate statistics? Are either of these companies strong in support before, during and after GO LIVE? Can you incorporate PDF files into reports or access information from the HIS into the LIS with little or no hand to computer combat? These are just surface type questions, but I would truly appreciate anyone's input. Thank you and have a nice weekend. Vikki Baker From gu.lang at gmx.at Sat Nov 18 04:00:17 2017 From: gu.lang at gmx.at (Gudrun Lang) Date: Sat, 18 Nov 2017 11:00:17 +0100 Subject: [Histonet] Ventana special stainer Message-ID: <002a01d36054$0a4e0140$1eea03c0$@gmx.at> Hello! I have a question for those, who have experience with the "old" Ventana special stainer Nexes and switched to the "new" special stainer Benchmark. Is there a difference in the quality of the stainings? Better reagenses? More possibilities to adapt the protocols? Or is it just the same and only the deparaffination is now on board? Thanks in advance Gudrun Lang From estellamireles at gmail.com Sat Nov 18 07:49:27 2017 From: estellamireles at gmail.com (Stella Mireles) Date: Sat, 18 Nov 2017 07:49:27 -0600 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: Please unsubscribe me. I tried this on your site, but still receiving messages. From isabelsoto1162 at yahoo.com Sat Nov 18 07:51:09 2017 From: isabelsoto1162 at yahoo.com (Isabel Soto) Date: Sat, 18 Nov 2017 13:51:09 +0000 (UTC) Subject: [Histonet] Ventana special stainer In-Reply-To: <002a01d36054$0a4e0140$1eea03c0$@gmx.at> References: <002a01d36054$0a4e0140$1eea03c0$@gmx.at> Message-ID: <1466108988.681372.1511013069483@mail.yahoo.com> Yes.. just make sure you don't fill the wash to full capacity and change that was every other day. That way it works beautiful? Sent from Yahoo Mail on Android On Sat, Nov 18, 2017 at 5:20 AM, Gudrun Lang via Histonet wrote: Hello! I have a question for those, who have experience with the "old" Ventana special stainer Nexes and switched to the "new" special stainer Benchmark. Is there a difference in the quality of the stainings? Better reagenses? More possibilities to adapt the protocols? Or is it just the same and only the deparaffination is now on board? Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garethdavisyuma at gmail.com Mon Nov 20 10:36:41 2017 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Mon, 20 Nov 2017 09:36:41 -0700 Subject: [Histonet] Specimen's from hospital to lab. Message-ID: Are there any labs that are private, but receive specimens from a hospital? If so, what kind of protocol or policy do you give the hospital to follow in order for them to submit specimens to the lab? Thanks, -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From jpiche at wtbyhosp.org Tue Nov 21 08:17:36 2017 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Tue, 21 Nov 2017 14:17:36 +0000 Subject: [Histonet] PAX-8 Message-ID: <631955447A364B45B9458D2905635110015C1FEABC@WIN08-MBX-02.wtbyhosp.org> Good Morning, Is anyone using PAX-8 from Biocare on the Dako Omnis and if so would you mind sharing your protocol/control tissue? I'll also take any information on any Pax8 protocols from different vendors/instruments. Thank you, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From jpiche at wtbyhosp.org Tue Nov 21 08:20:43 2017 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Tue, 21 Nov 2017 14:20:43 +0000 Subject: [Histonet] grossing credentials? Message-ID: <631955447A364B45B9458D2905635110015C1FEACE@WIN08-MBX-02.wtbyhosp.org> Good Morning, One more question. I was wondering if anyone is having a problem being credentialed for grossing with an Associate in Science in Veterinary Technology? Our state DPH is questioning my degree as not being a laboratory technology. I know there are lots of techs working in veterinary histology as well so I was wondering what you would make of this. Thank you and happy Thanksgiving! Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From Linda.Margraf at cookchildrens.org Tue Nov 21 11:37:22 2017 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Tue, 21 Nov 2017 17:37:22 +0000 Subject: [Histonet] Histotech Need Chesapeake Urology Message-ID: Here is a message I am posting for a subscriber. Linda M Histonet administrator --------------------------------------------------------------------------- Hi Linda, My initial email message got rejected. Can you please post our opening? Mike About Us: Chesapeake Urology is the largest urology practice in the Mid-Atlantic region, providing a comprehensive array of urologic services and treatment options to patients. As one of the most respected and progressive urology practices in the nation, we have over 100 providers throughout our 26 medical offices and 17 outpatient surgical centers. Working in a team environment focused on patient care, our mission is to ensure every patient receives a superior experience. As one of the "Top 100 Best Places to Work in Health Care" and voted a "Best Place to Work" by multiple organizations, we maintain our exceptional patient care and culture by selecting the best people in our field, promoting a healthy and respectful environment, and serving the community through our outreach programs and events. We are proud that our people make us the best and it shows in the superb care we deliver to each and every patient. For more information, please visit our website at www.chesapeakeurology.com. We are seeking a full-time Histotechnologist for our new Pathology Lab located in Beltsville, MD. This position supports the pathology histology laboratory in preparing, processing, and staining of tissue specimens for review by the pathologists. The hours are Monday-Friday and can be flexible in the afternoon/evening. Responsibilities: *Responsible for accessioning, grossing, embedding, microtomy, performing H&E and papanicolau staining, and cover slipping of all surgical/cytologic specimen slides for microscopic analysis. *Loading the blocks on the processor ensuring quality and timeliness of production. *Modify and validate procedures and methodologies for immunohistochemistry, special staining and other related staining. *Organize cases by matching slides with appropriate paperwork and/or laying slides out in slide folders. *Provide maintenance or troubleshooting of equipment and automated instrumentation within the laboratory. *Assists supervisor in meeting department regulatory agency requirements (CAP, CLIA, etc.) *Performs quality control procedures per protocol and completes necessary documentation. *Maintain supplies and other inventory. *Perform other laboratory and office duties as assigned by manager. Requirements *An Associate's Degree in a laboratory science or medical laboratory technology obtained from an accredited institution or education/training equivalent to the above that includes at least 60 semester hours from an accredited institution. *HT or HTL certification required. *Proficient embedding, sectioning and histology techniques with human tissues. From kjohnson at moellerdermatology.com Tue Nov 21 14:35:21 2017 From: kjohnson at moellerdermatology.com (Kristina Johnson) Date: Tue, 21 Nov 2017 14:35:21 -0600 Subject: [Histonet] Histonet Digest, Vol 168, Issue 17 In-Reply-To: References: Message-ID: This is a long shot, but I am moving to Rogers Arkansas and I was wondering if anyone knew of any Histology jobs in the area. Iv been looking for two months, but as we all know, Histologists stay put so not many jobs come open. Thanks in advance for any help. On Tue, Nov 21, 2017 at 12:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. PAX-8 (Piche, Jessica) > 2. grossing credentials? (Piche, Jessica) > 3. Histotech Need Chesapeake Urology (Linda Margraf) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 21 Nov 2017 14:17:36 +0000 > From: "Piche, Jessica" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] PAX-8 > Message-ID: > <631955447A364B45B9458D2905635110015C1FEABC at WIN08-MBX-02. > wtbyhosp.org> > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > > Is anyone using PAX-8 from Biocare on the Dako Omnis and if so would you > mind sharing your protocol/control tissue? I'll also take any information > on any Pax8 protocols from different vendors/instruments. > > Thank you, > > Jessica Piche, HT(ASCP) > Waterbury Hospital > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain > confidential information that is legally privileged. This information is > intended only for the use of the individual or entity named above. The > authorized recipient of this information is prohibited from disclosing this > information to any other party unless required to do so by law or > regulation. If you are not the intended recipient, you are hereby notified > that any disclosure, copying, distribution or action taken in reliance on > the contents of these documents is strictly prohibited. If you have > received this information in error, please notify the sender immediately > and delete these documents. Copyright (c) Waterbury Hospital > > > ------------------------------ > > Message: 2 > Date: Tue, 21 Nov 2017 14:20:43 +0000 > From: "Piche, Jessica" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] grossing credentials? > Message-ID: > <631955447A364B45B9458D2905635110015C1FEACE at WIN08-MBX-02. > wtbyhosp.org> > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > > One more question. I was wondering if anyone is having a problem being > credentialed for grossing with an Associate in Science in Veterinary > Technology? Our state DPH is questioning my degree as not being a > laboratory technology. I know there are lots of techs working in veterinary > histology as well so I was wondering what you would make of this. > > Thank you and happy Thanksgiving! > > Jessica Piche, HT(ASCP) > Waterbury Hospital > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain > confidential information that is legally privileged. This information is > intended only for the use of the individual or entity named above. The > authorized recipient of this information is prohibited from disclosing this > information to any other party unless required to do so by law or > regulation. If you are not the intended recipient, you are hereby notified > that any disclosure, copying, distribution or action taken in reliance on > the contents of these documents is strictly prohibited. If you have > received this information in error, please notify the sender immediately > and delete these documents. Copyright (c) Waterbury Hospital > > > ------------------------------ > > Message: 3 > Date: Tue, 21 Nov 2017 17:37:22 +0000 > From: Linda Margraf > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Histotech Need Chesapeake Urology > Message-ID: > Content-Type: text/plain; charset="utf-8" > > Here is a message I am posting for a subscriber. > Linda M > Histonet administrator > > ------------------------------------------------------------ > --------------- > Hi Linda, > > My initial email message got rejected. Can you please post our opening? > > Mike > > About Us: Chesapeake Urology is the largest urology practice in the > Mid-Atlantic region, providing a comprehensive array of urologic services > and treatment options to patients. As one of the most respected and > progressive urology practices in the nation, we have over 100 providers > throughout our 26 medical offices and 17 outpatient surgical centers. > Working in a team environment focused on patient care, our mission is to > ensure every patient receives a superior experience. > > As one of the "Top 100 Best Places to Work in Health Care" and voted a > "Best Place to Work" by multiple organizations, we maintain our exceptional > patient care and culture by selecting the best people in our field, > promoting a healthy and respectful environment, and serving the community > through our outreach programs and events. We are proud that our people make > us the best and it shows in the superb care we deliver to each and every > patient. For more information, please visit our website at > www.chesapeakeurology.com. > We are seeking a full-time Histotechnologist for our new Pathology Lab > located in Beltsville, MD. This position supports the pathology histology > laboratory in preparing, processing, and staining of tissue specimens for > review by the pathologists. The hours are Monday-Friday and can be flexible > in the afternoon/evening. > > Responsibilities: > *Responsible for accessioning, grossing, embedding, microtomy, performing > H&E and papanicolau staining, and cover slipping of all surgical/cytologic > specimen slides for microscopic analysis. > *Loading the blocks on the processor ensuring quality and timeliness of > production. > *Modify and validate procedures and methodologies for > immunohistochemistry, special staining and other related staining. > *Organize cases by matching slides with appropriate paperwork and/or > laying slides out in slide folders. > *Provide maintenance or troubleshooting of equipment and automated > instrumentation within the laboratory. > *Assists supervisor in meeting department regulatory agency requirements > (CAP, CLIA, etc.) *Performs quality control procedures per protocol and > completes necessary documentation. > *Maintain supplies and other inventory. > *Perform other laboratory and office duties as assigned by manager. > > Requirements > *An Associate's Degree in a laboratory science or medical laboratory > technology obtained from an accredited institution or education/training > equivalent to the above that includes at least 60 semester hours from an > accredited institution. > *HT or HTL certification required. > *Proficient embedding, sectioning and histology techniques with human > tissues. > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 168, Issue 17 > ***************************************** > -- *Kristina Johnson, HT (ASPC)* Histologist Moeller Dermatology, LLC 1911 N. Webb Road Wichita, KS 67206 ? (316)682-7546 Ext:246 ? (316)682-7554 www.moellerdermatology.com Facebook Twitter This e-mail, and any attachments thereto, is intended only for use by the addressee(s) named herein and may contain legally privileged and/or confidential information. If you are not the intended recipient of this e-mail (or the person responsible for delivering this document to the intended recipient), you are hereby notified that any dissemination, distribution, printing or copying of this e-mail, and any attachments thereto, is strictly prohibited. If you have received this e-mail in error, please respond to the individual sending the message and permanently delete the original and any copy of any e-mail and any printout thereof From Scott.Lindrud at rice.willmar.mn.us Tue Nov 21 15:05:51 2017 From: Scott.Lindrud at rice.willmar.mn.us (Lindrud, Scott) Date: Tue, 21 Nov 2017 21:05:51 +0000 Subject: [Histonet] Negative IHC Control for Her2 Message-ID: <8da687dd9ab34d6b9b153c9c2b2f145c@RMH-MAIL.ricehospital.local> Hi All, We are having an internal debate regarding Her2 IHC control tissue in our lab. We run a MTA (multi tissue array) consisting of 0+, 1+, 2+, and 3+ Her2 staining tissue taken from lumpectomy/resection cases. I'm in the process of searching for more tissue to use in future control blocks and it can be difficult to find tissue that is 0+ and 3+. I've discussed this with our pathologist in charge of Histology and he says that we don't need to run all 4 of the cores as controls. He says all we need to run is a positive control and a negative control. He says the positive control could be a 2+ or 3+ and the negative control could be either a 0+ OR a 1+. I respectfully disagreed with him and said the negative control is not meant for accessing staining interpretation but to verify the sensitivity and specificity of the actual antibody-antigen reaction. I said a 1+ is still staining the tissue where there is no staining in a 0+ reaction. The 1+ is not a negative staining reaction, but a negative interpretation. The pathologist says I'm wrong. The CAP in its checklist says "It is also important to assess the specificity of each antibody by a negative tissue control, which must show no staining of tissues known to lack the antigen". To me, a 1+ Her2 staining reaction shows that that tissue has antigen and should not be used as a negative control. So, after saying all that, can/should 1+ Her2 breast tissue be used as a negative tissue control? Seems pretty straight forward to me, but I'm just a Cytotechnologist/Histotech. Thanks for any input! Scott Scott A. Lindrud, MLS(ASCP)CM CTCM Histopathology Technical Specialist/Cytotechnologist Rice Memorial Hospital 301 Becker Ave SW Willmar, MN 56201 WP(320)231-4406 Fax(320)231-4861 scott.lindrud at rice.willmar.mn.us ________________________________ "This e-mail transmission is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution violates confidentiality and privacy laws and is prohibited. If you are not the intended recipient, please notify the sender immediately and delete all copies of the message. Thank you." From rjbuesa at yahoo.com Wed Nov 22 10:02:21 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 22 Nov 2017 16:02:21 +0000 (UTC) Subject: [Histonet] Negative IHC Control for Her2 In-Reply-To: <8da687dd9ab34d6b9b153c9c2b2f145c@RMH-MAIL.ricehospital.local> References: <8da687dd9ab34d6b9b153c9c2b2f145c@RMH-MAIL.ricehospital.local> Message-ID: <800857894.1231622.1511366541476@mail.yahoo.com> You are right but, your point is?Regardless, the pathologist is the responsible for his/her diagnosis/interpretation and the liability is his/hers.Remember that for us histotechs, our "client" is the pathologist (always remembering the patient behind the whole process) and our essential duty is to provide the pathologist what s/he needs to being able to make the best and more accurate diagnosis.Follow his/her instructions and your life will be much simpler and less stressful.Ren? On Tuesday, November 21, 2017 4:21 PM, "Lindrud, Scott via Histonet" wrote: Hi All, We are having an internal debate regarding Her2 IHC control tissue in our lab.? We run a MTA (multi tissue array) consisting of 0+, 1+, 2+, and 3+ Her2 staining tissue taken from lumpectomy/resection cases.? I'm in the process of searching for more tissue to use in future control blocks and it can be difficult to find tissue that is 0+ and 3+. I've discussed this with our pathologist in charge of Histology and he says that we don't need to run all 4 of the cores as controls.? He says all we need to run is a positive control and a negative control.? He says the positive control could be a 2+ or 3+ and the negative control could be either a 0+ OR a 1+. I respectfully disagreed with him and said the negative control is not meant for accessing staining interpretation but to verify the sensitivity and specificity of the actual antibody-antigen reaction.? I said a 1+ is still staining the tissue where there is no staining in a 0+ reaction.? The 1+ is not a negative staining reaction, but a negative interpretation.? The pathologist says I'm wrong. The CAP in its checklist says "It is also important to assess the specificity of each antibody by a negative tissue control, which must show no staining of tissues known to lack the antigen".? ? To me, a 1+ Her2 staining reaction shows that that tissue has antigen and should not be used as a negative control. So, after saying all that, can/should? 1+ Her2 breast tissue be used as a negative tissue control?? Seems pretty straight forward to me, but I'm just a Cytotechnologist/Histotech. Thanks for any input! Scott Scott A. Lindrud, MLS(ASCP)CM CTCM Histopathology Technical Specialist/Cytotechnologist Rice Memorial Hospital 301 Becker Ave SW Willmar, MN 56201 WP(320)231-4406 Fax(320)231-4861 scott.lindrud at rice.willmar.mn.us ________________________________ "This e-mail transmission is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution violates confidentiality and privacy laws and is prohibited. If you are not the intended recipient, please notify the sender immediately and delete all copies of the message. Thank you." _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet | | Virus-free. www.avast.com | From tbraud at holyredeemer.com Wed Nov 22 13:21:55 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 22 Nov 2017 19:21:55 +0000 Subject: [Histonet] Her 2 Control Message-ID: <48E053DDF6CE074DB6A7414BA05403F84CE9F7A5@HRHEX03-HOS.holyredeemer.local> Hey Scott - Our interpretation of CAP requirements for IHC Controls is the same as yours. Our Her2 control is a microarray with 3+ case and a piece of normal skin to serve as our negative tissue control. If you have normal breast tissue adjacent to your tumor control, that can also serve as a negative tissue control, but your procedure has to state it as such. Our control tissue are 5mm punches of solid tumor, so we just add a punch of normal skin. I hope this helps, but sometimes, it's just not worth the argument. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Message: 2 Date: Tue, 21 Nov 2017 21:05:51 +0000 From: "Lindrud, Scott" Subject: [Histonet] Negative IHC Control for Her2 Hi All, We are having an internal debate regarding Her2 IHC control tissue in our lab. We run a MTA (multi tissue array) consisting of 0+, 1+, 2+, and 3+ Her2 staining tissue taken from lumpectomy/resection cases. I'm in the process of searching for more tissue to use in future control blocks and it can be difficult to find tissue that is 0+ and 3+. I've discussed this with our pathologist in charge of Histology and he says that we don't need to run all 4 of the cores as controls. He says all we need to run is a positive control and a negative control. He says the positive control could be a 2+ or 3+ and the negative control could be either a 0+ OR a 1+. I respectfully disagreed with him and said the negative control is not meant for accessing staining interpretation but to verify the sensitivity and specificity of the actual antibody-antigen reaction. I said a 1+ is still staining the tissue where there is no staining in a 0+ reaction. The 1+ is not a negative staining reaction, but a negative interpretation. The pathologist says I'm wrong. The CAP in its checklist says "It is also important to assess the specificity of each antibody by a negative tissue control, which must show no staining of tissues known to lack the antigen". To me, a 1+ Her2 staining reaction shows that that tissue has antigen and should not be used as a negative control. So, after saying all that, can/should 1+ Her2 breast tissue be used as a negative tissue control? Seems pretty straight forward to me, but I'm just a Cytotechnologist/Histotech. Thanks for any input! Scott Scott A. Lindrud, MLS(ASCP)CM CTCM Histopathology Technical Specialist/Cytotechnologist Rice Memorial Hospital 301 Becker Ave SW Willmar, MN 56201 WP(320)231-4406 Fax(320)231-4861 scott.lindrud at rice.willmar.mn.us ________________________________ "This e-mail transmission is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution violates confidentiality and privacy laws and is prohibited. If you are not the intended recipient, please notify the sender immediately and delete all copies of the message. Thank you." ------------------------------ Message: 3 Date: Wed, 22 Nov 2017 16:02:21 +0000 (UTC) From: Rene J Buesa To: "Lindrud, Scott" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Negative IHC Control for Her2 Message-ID: <800857894.1231622.1511366541476 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 You are right but, your point is?Regardless, the pathologist is the responsible for his/her diagnosis/interpretation and the liability is his/hers.Remember that for us histotechs, our "client" is the pathologist (always remembering the patient behind the whole process) and our essential duty is to provide the pathologist what s/he needs to being able to make the best and more accurate diagnosis.Follow his/her instructions and your life will be much simpler and less stressful.Ren? On Tuesday, November 21, 2017 4:21 PM, "Lindrud, Scott via Histonet" wrote: Hi All, We are having an internal debate regarding Her2 IHC control tissue in our lab.? We run a MTA (multi tissue array) consisting of 0+, 1+, 2+, and 3+ Her2 staining tissue taken from lumpectomy/resection cases.? I'm in the process of searching for more tissue to use in future control blocks and it can be difficult to find tissue that is 0+ and 3+. I've discussed this with our pathologist in charge of Histology and he says that we don't need to run all 4 of the cores as controls.? He says all we need to run is a positive control and a negative control.? He says the positive control could be a 2+ or 3+ and the negative control could be either a 0+ OR a 1+. I respectfully disagreed with him and said the negative control is not meant for accessing staining interpretation but to verify the sensitivity and specificity of the actual antibody-antigen reaction.? I said a 1+ is still staining the tissue where there is no staining in a 0+ reaction.? The 1+ is not a negative staining reaction, but a negative interpretation.? The pathologist says I'm wrong. The CAP in its checklist says "It is also important to assess the specificity of each antibody by a negative tissue control, which must show no staining of tissues known to lack the antigen".? ? To me, a 1+ Her2 staining reaction shows that that tissue has antigen and should not be used as a negative control. So, after saying all that, can/should? 1+ Her2 breast tissue be used as a negative tissue control?? Seems pretty straight forward to me, but I'm just a Cytotechnologist/Histotech. Thanks for any input! Scott Scott A. Lindrud, MLS(ASCP)CM CTCM Histopathology Technical Specialist/Cytotechnologist Rice Memorial Hospital 301 Becker Ave SW Willmar, MN 56201 WP(320)231-4406 Fax(320)231-4861 scott.lindrud at rice.willmar.mn.us ________________________________ "This e-mail transmission is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution violates confidentiality and privacy laws and is prohibited. If you are not the intended recipient, please notify the sender immediately and delete all copies of the message. Thank you." _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet | | Virus-free. www.avast.com | ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 168, Issue 18 ***************************************** From portera at msu.edu Wed Nov 22 16:31:12 2017 From: portera at msu.edu (Amy Porter) Date: Wed, 22 Nov 2017 17:31:12 -0500 Subject: [Histonet] Cytochrome Oxidase Enzyme Histochemical Staining Message-ID: <000001d363e1$9a463480$ced29d80$@edu> Anyone out there doing COX enzyme histochemistry on fresh flash frozen EQUINE muscle...I have tried several methods and fibers are not differentiating the way they should be and are very faint ..any suggestions welcome!! Thanks - Amy Amy S. Porter, HT (ASCP) Michigan State University - Department of Physiology Investigative HistoPathology Lab - Supervisor Research Core Support Facility 567 Wilson Road - Room 2201 East Lansing, MI 48824-6458 517-884-5026 portera at msu.edu From garethdavisyuma at gmail.com Mon Nov 27 16:38:12 2017 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Mon, 27 Nov 2017 15:38:12 -0700 Subject: [Histonet] Fwd: Specimen's from hospital to lab. In-Reply-To: References: Message-ID: I sent this question out last week, someone answered and I guess I accidentally deleted the email. So, if anyone remembers answering my question about private labs doing hospital outpatient biopsies, please email me again. Thanks, Gareth Davis ---------- Forwarded message ---------- From: Gareth Davis Date: Mon, Nov 20, 2017 at 9:36 AM Subject: Specimen's from hospital to lab. To: "Histonet at lists.utsouthwestern.edu" Are there any labs that are private, but receive specimens from a hospital? If so, what kind of protocol or policy do you give the hospital to follow in order for them to submit specimens to the lab? Thanks, -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 <(928)%20248-5259> -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From alkhenaizik at gmail.com Tue Nov 28 08:02:38 2017 From: alkhenaizik at gmail.com (kalkhenaizi .) Date: Tue, 28 Nov 2017 17:02:38 +0300 Subject: [Histonet] SV40 control tissue/slides Message-ID: Hello histonetters, Can someone advise where I can get SV40 control tissue/slides? Thanks, Kadhem ExpressMed Labs Bahrain From naje1972 at yahoo.com Tue Nov 28 09:43:36 2017 From: naje1972 at yahoo.com (cynthia haynes) Date: Tue, 28 Nov 2017 15:43:36 +0000 (UTC) Subject: [Histonet] SV40 control tissue/slides In-Reply-To: References: Message-ID: <1361890548.2794108.1511883816564@mail.yahoo.com> Hello KachemakPleasecontact me at naje1972 at yahoo.com.?I think I can help you with obtaining this control tissue. Cynthia James H.T? Sent from Yahoo Mail on Android On Tue, Nov 28, 2017 at 8:16 AM, kalkhenaizi . via Histonet wrote: Hello histonetters, Can someone advise where I can get SV40 control tissue/slides? Thanks, Kadhem ExpressMed Labs Bahrain _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcdukes at lexhealth.org Tue Nov 28 10:09:49 2017 From: bcdukes at lexhealth.org (Blake Taylor) Date: Tue, 28 Nov 2017 16:09:49 +0000 Subject: [Histonet] Beaker with or without Vantage Message-ID: I'm looking for anyone out there that has switched to Beaker for AP, Also do you use Ventana Connect with Beaker? Did you choose to use the Beaker tracking system or is anyone using Beaker in conjunction with Vantage? Our Hospital is in the beginning phase of moving from Copath Sunquest to Beaker (2017 version) . Any thoughts of what has gone well and what has not would be appreciated. Thanks so much Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes at lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use to the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. From redrose297 at gmail.com Tue Nov 28 10:25:25 2017 From: redrose297 at gmail.com (warda hassan) Date: Tue, 28 Nov 2017 16:25:25 +0000 Subject: [Histonet] Dako Omnis V/S Ultra-Ventana Message-ID: Hello to all We are currently working on Dako Flex with work load of 10 thousand immune slides excluding FISH or ISH. Planning to move to new system, i would request if all can share their feed back on which system is better among the available options from market like ULTRA VENTANA OR DAKO OMNIS! Thank you in Advance From Timothy.Morken at ucsf.edu Tue Nov 28 10:48:09 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 28 Nov 2017 16:48:09 +0000 Subject: [Histonet] Beaker with or without Vantage In-Reply-To: References: Message-ID: I am interested as well. "They" are threatening us with a move to Beaker in the future.... Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Blake Taylor via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 28, 2017 8:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Beaker with or without Vantage I'm looking for anyone out there that has switched to Beaker for AP, Also do you use Ventana Connect with Beaker? Did you choose to use the Beaker tracking system or is anyone using Beaker in conjunction with Vantage? Our Hospital is in the beginning phase of moving from Copath Sunquest to Beaker (2017 version) . Any thoughts of what has gone well and what has not would be appreciated. Thanks so much Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes at lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use to the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz at premierlab.com Tue Nov 28 11:18:48 2017 From: liz at premierlab.com (Liz Chlipala) Date: Tue, 28 Nov 2017 17:18:48 +0000 Subject: [Histonet] Beaker with or without Vantage In-Reply-To: References: Message-ID: I'm a member of API - Association for Pathology Informatics and they have a list server similar to the histonet and there is always posts about Epic Beaker. You might find some information on their website. https://www.pathologyinformatics.org/ Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 28, 2017 9:48 AM To: Histonet Subject: Re: [Histonet] Beaker with or without Vantage I am interested as well. "They" are threatening us with a move to Beaker in the future.... Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Blake Taylor via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 28, 2017 8:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Beaker with or without Vantage I'm looking for anyone out there that has switched to Beaker for AP, Also do you use Ventana Connect with Beaker? Did you choose to use the Beaker tracking system or is anyone using Beaker in conjunction with Vantage? Our Hospital is in the beginning phase of moving from Copath Sunquest to Beaker (2017 version) . Any thoughts of what has gone well and what has not would be appreciated. Thanks so much Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes at lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use to the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From Richard.Cartun at hhchealth.org Tue Nov 28 11:45:36 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 28 Nov 2017 17:45:36 +0000 Subject: [Histonet] I am trying to contact .... Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E95494EB6@HHCEXCHMB03.hhcsystem.org> Diane Cadorette-Hall. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From mward at wakehealth.edu Tue Nov 28 12:30:57 2017 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Tue, 28 Nov 2017 18:30:57 +0000 Subject: [Histonet] Beaker with or without Vantage In-Reply-To: References: Message-ID: "They" aren't the ones that will have to use it.....therein lies the rub! I have not heard many positive things about AP Beaker. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward at wakehealth.edu ? ? ? -----Original Message----- From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 28, 2017 11:48 AM To: Histonet Subject: Re: [Histonet] Beaker with or without Vantage I am interested as well. "They" are threatening us with a move to Beaker in the future.... Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Blake Taylor via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 28, 2017 8:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Beaker with or without Vantage I'm looking for anyone out there that has switched to Beaker for AP, Also do you use Ventana Connect with Beaker? Did you choose to use the Beaker tracking system or is anyone using Beaker in conjunction with Vantage? Our Hospital is in the beginning phase of moving from Copath Sunquest to Beaker (2017 version) . Any thoughts of what has gone well and what has not would be appreciated. Thanks so much Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes at lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use to the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From julio.benavides.silvan at csic.es Tue Nov 28 14:27:21 2017 From: julio.benavides.silvan at csic.es (Julio Benavides =?utf-8?b?U2lsdsOhbg==?=) Date: Tue, 28 Nov 2017 21:27:21 +0100 Subject: [Histonet] gross photography Message-ID: <20171128212721.Horde.fKyuS_h9MUojs2jLkgiLAg7@webmail.csic.es> Hi there, May I ask you your opinion about which system you are using to take gross pictures? We are using a couple of big tungsten light bulbs and a Nikon d60 camera. We are a research lab working with sheep, so we get big lesions in big organs. I was wondering if anybody is using a Digital Gross Photography System and how they compare with a "more ytraditional" digital camera approach. As always, thank you so much for your opinions. Greatly appreciated! Cheers Julio From hhawkins at UTMB.EDU Tue Nov 28 15:41:19 2017 From: hhawkins at UTMB.EDU (Hawkins, Hal K.) Date: Tue, 28 Nov 2017 21:41:19 +0000 Subject: [Histonet] gross photography In-Reply-To: <20171128212721.Horde.fKyuS_h9MUojs2jLkgiLAg7@webmail.csic.es> References: <20171128212721.Horde.fKyuS_h9MUojs2jLkgiLAg7@webmail.csic.es> Message-ID: <22624908330375439D6382C9F95093FF9C886175@GRMBX1.utmb.edu> We have used handheld digital cameras for our research in sheep in Galveston. For autopsies at the Shriners hospital, we use handheld cameras, one operated by a professional photographer, and also an old copy stand with hot lights and a backlight and a Sony digital camera with a macro lens on an alpha lens mount adapter, which works pretty well. The new macro photography in the UTMB autopsy service is great -- you enter the case number and put the specimen on the stand, and the system handles focus, exposure and record keeping and provides excellent pictures. ________________________________________ From: Julio Benavides Silv?n via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 28, 2017 2:27 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] gross photography Hi there, May I ask you your opinion about which system you are using to take gross pictures? We are using a couple of big tungsten light bulbs and a Nikon d60 camera. We are a research lab working with sheep, so we get big lesions in big organs. I was wondering if anybody is using a Digital Gross Photography System and how they compare with a "more ytraditional" digital camera approach. As always, thank you so much for your opinions. Greatly appreciated! Cheers Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Chhawkins%40utmb.edu%7C8ff8b283cca348a1554608d5369e89a5%7C7bef256d85db4526a72d31aea2546852%7C0%7C0%7C636474976899839861&sdata=HRHmgMeDg4omjsN%2BIbKBFquL21G7uBM778ypJJ8JiOQ%3D&reserved=0 From Ana.Maluenda at baker.edu.au Wed Nov 29 00:41:32 2017 From: Ana.Maluenda at baker.edu.au (Ana Maluenda) Date: Wed, 29 Nov 2017 06:41:32 +0000 Subject: [Histonet] Frozen section - IHC for 6x HisTag Message-ID: Hi everyone! Does anyone have experience with immunohistochemistry or immunofluorescence anti-6x HisTag on frozen sections, mouse tissue? I can find online lots of references for immunocytochemistry, but not much on tissue staining. Any help or hints would be much appreciated! Kind regards, Ana Ana Maluenda Research Assistant Atherothrombosis and Vascular Biology Laboratory Baker Heart and Diabetes Institute 75 Commercial Road, Melbourne VIC 3004 P (03) 8532 1359 E Ana.Maluenda at baker.edu.au W www.baker.edu.au Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting privacy at baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg From LRaff at uropartners.com Wed Nov 29 08:41:42 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 29 Nov 2017 14:41:42 +0000 Subject: [Histonet] Lab related blog post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF1173C28F@COLOEXCH01.uropartners.local> For those interested, Part 1 of a two part blog about pathology and diagnosis. http://www.chicagonow.com/downsize-maybe/2017/11/i-give-people-cancer-part-1/ Have a good week. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From gu.lang at gmx.at Wed Nov 29 10:13:38 2017 From: gu.lang at gmx.at (Gudrun Lang) Date: Wed, 29 Nov 2017 17:13:38 +0100 Subject: [Histonet] gross photography In-Reply-To: <20171128212721.Horde.fKyuS_h9MUojs2jLkgiLAg7@webmail.csic.es> References: <20171128212721.Horde.fKyuS_h9MUojs2jLkgiLAg7@webmail.csic.es> Message-ID: <001b01d3692d$04822840$0d8678c0$@gmx.at> Hi, We use MakroPath from Milestone in a routine histolab. The camera is mounted on the top oft he grossing-station, with an integrated PC+monitor and pedals for zooming and taking photos. Within this system you can mark the pictures, draw something, measure something ... In comparison to the older method with digital-camera, manual zoom etc. it is very conveniant. Picture quality is high. Gudrun -----Urspr?ngliche Nachricht----- Von: Julio Benavides Silv?n via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Dienstag, 28. November 2017 21:27 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] gross photography Hi there, May I ask you your opinion about which system you are using to take gross pictures? We are using a couple of big tungsten light bulbs and a Nikon d60 camera. We are a research lab working with sheep, so we get big lesions in big organs. I was wondering if anybody is using a Digital Gross Photography System and how they compare with a "more ytraditional" digital camera approach. As always, thank you so much for your opinions. Greatly appreciated! Cheers Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mark.Pawlowski at jefferson.edu Wed Nov 29 13:15:20 2017 From: Mark.Pawlowski at jefferson.edu (Mark Pawlowski) Date: Wed, 29 Nov 2017 19:15:20 +0000 Subject: [Histonet] Beaker In-Reply-To: References: Message-ID: Good luck with Beaker. We switched in April and had many issues. Insist on a complete run through of a specimen, from collecting to billing before you go live. We had issues where our pathologist did not have access, printers were not linked, etc. Get used to the phrase "It's hard coded" which is used by the Beaker folks when something cannot be changed. The interface is more involved than with our homegrown database and now almost eight months later it is taking us about double the time to do the data entry. Also, schedule a 'At the elbow support person' to be in your lab area a full week when you go live. This is a Beaker term for someone who has experience using the system from another organization. And finally, get ready to start a lot of tickets, which is the way that your get something addressed in EPIC. The first day, I put in 34. Mark ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Wednesday, November 29, 2017 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 168, Issue 21 WARNING: External Email - This email originated outside of Jefferson. DO NOT CLICK links or attachments unless you recognize the sender and are expecting the email. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cmark.pawlowski%40jefferson.edu%7C1f1f96b2c5c44daf587a08d537531325%7C55a89906c710436bbc444c590cb67c4a%7C0%7C0%7C636475752295278455&sdata=IscLSn7EPmltul9348k2X53IdXsy54jgWu7e8MvupXU%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Beaker with or without Vantage (Martha Ward-Pathology) 2. gross photography (Julio Benavides Silv?n) 3. Re: gross photography (Hawkins, Hal K.) 4. Frozen section - IHC for 6x HisTag (Ana Maluenda) 5. Lab related blog post (Lester Raff MD) 6. Re: gross photography (Gudrun Lang) ---------------------------------------------------------------------- Message: 1 Date: Tue, 28 Nov 2017 18:30:57 +0000 From: Martha Ward-Pathology To: "Morken, Timothy" Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Beaker with or without Vantage Message-ID: Content-Type: text/plain; charset="iso-8859-1" "They" aren't the ones that will have to use it.....therein lies the rub! I have not heard many positive things about AP Beaker. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward at wakehealth.edu ? ? ? -----Original Message----- From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 28, 2017 11:48 AM To: Histonet Subject: Re: [Histonet] Beaker with or without Vantage I am interested as well. "They" are threatening us with a move to Beaker in the future.... Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Blake Taylor via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 28, 2017 8:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Beaker with or without Vantage I'm looking for anyone out there that has switched to Beaker for AP, Also do you use Ventana Connect with Beaker? Did you choose to use the Beaker tracking system or is anyone using Beaker in conjunction with Vantage? Our Hospital is in the beginning phase of moving from Copath Sunquest to Beaker (2017 version) . Any thoughts of what has gone well and what has not would be appreciated. Thanks so much Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes at lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use to the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cmark.pawlowski%40jefferson.edu%7C1f1f96b2c5c44daf587a08d537531325%7C55a89906c710436bbc444c590cb67c4a%7C0%7C0%7C636475752295278455&sdata=IscLSn7EPmltul9348k2X53IdXsy54jgWu7e8MvupXU%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cmark.pawlowski%40jefferson.edu%7C1f1f96b2c5c44daf587a08d537531325%7C55a89906c710436bbc444c590cb67c4a%7C0%7C0%7C636475752295278455&sdata=IscLSn7EPmltul9348k2X53IdXsy54jgWu7e8MvupXU%3D&reserved=0 ------------------------------ Message: 2 Date: Tue, 28 Nov 2017 21:27:21 +0100 From: Julio Benavides Silv?n To: histonet at lists.utsouthwestern.edu Subject: [Histonet] gross photography Message-ID: <20171128212721.Horde.fKyuS_h9MUojs2jLkgiLAg7 at webmail.csic.es> Content-Type: text/plain; charset=UTF-8; format=flowed; DelSp=Yes Hi there, May I ask you your opinion about which system you are using to take gross pictures? We are using a couple of big tungsten light bulbs and a Nikon d60 camera. We are a research lab working with sheep, so we get big lesions in big organs. I was wondering if anybody is using a Digital Gross Photography System and how they compare with a "more ytraditional" digital camera approach. As always, thank you so much for your opinions. Greatly appreciated! Cheers Julio ------------------------------ Message: 3 Date: Tue, 28 Nov 2017 21:41:19 +0000 From: "Hawkins, Hal K." To: Julio Benavides Silv?n , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] gross photography Message-ID: <22624908330375439D6382C9F95093FF9C886175 at GRMBX1.utmb.edu> Content-Type: text/plain; charset="iso-8859-1" We have used handheld digital cameras for our research in sheep in Galveston. For autopsies at the Shriners hospital, we use handheld cameras, one operated by a professional photographer, and also an old copy stand with hot lights and a backlight and a Sony digital camera with a macro lens on an alpha lens mount adapter, which works pretty well. The new macro photography in the UTMB autopsy service is great -- you enter the case number and put the specimen on the stand, and the system handles focus, exposure and record keeping and provides excellent pictures. ________________________________________ From: Julio Benavides Silv?n via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 28, 2017 2:27 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] gross photography Hi there, May I ask you your opinion about which system you are using to take gross pictures? We are using a couple of big tungsten light bulbs and a Nikon d60 camera. We are a research lab working with sheep, so we get big lesions in big organs. I was wondering if anybody is using a Digital Gross Photography System and how they compare with a "more ytraditional" digital camera approach. As always, thank you so much for your opinions. Greatly appreciated! Cheers Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Chhawkins%40utmb.edu%7C8ff8b283cca348a1554608d5369e89a5%7C7bef256d85db4526a72d31aea2546852%7C0%7C0%7C636474976899839861&sdata=HRHmgMeDg4omjsN%2BIbKBFquL21G7uBM778ypJJ8JiOQ%3D&reserved=0 ------------------------------ Message: 4 Date: Wed, 29 Nov 2017 06:41:32 +0000 From: Ana Maluenda To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Frozen section - IHC for 6x HisTag Message-ID: Content-Type: text/plain; charset="us-ascii" Hi everyone! Does anyone have experience with immunohistochemistry or immunofluorescence anti-6x HisTag on frozen sections, mouse tissue? I can find online lots of references for immunocytochemistry, but not much on tissue staining. Any help or hints would be much appreciated! Kind regards, Ana Ana Maluenda Research Assistant Atherothrombosis and Vascular Biology Laboratory Baker Heart and Diabetes Institute 75 Commercial Road, Melbourne VIC 3004 P (03) 8532 1359 E Ana.Maluenda at baker.edu.au W https://na01.safelinks.protection.outlook.com/?url=www.baker.edu.au&data=02%7C01%7Cmark.pawlowski%40jefferson.edu%7C1f1f96b2c5c44daf587a08d537531325%7C55a89906c710436bbc444c590cb67c4a%7C0%7C0%7C636475752295278455&sdata=VcZ0WYNpW31p4VJd2WSNKV8z8QapVHCQfawsT%2BCmiAM%3D&reserved=0 Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at https://na01.safelinks.protection.outlook.com/?url=www.baker.edu.au&data=02%7C01%7Cmark.pawlowski%40jefferson.edu%7C1f1f96b2c5c44daf587a08d537531325%7C55a89906c710436bbc444c590cb67c4a%7C0%7C0%7C636475752295278455&sdata=VcZ0WYNpW31p4VJd2WSNKV8z8QapVHCQfawsT%2BCmiAM%3D&reserved=0 or on request by contacting privacy at baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg ------------------------------ Message: 5 Date: Wed, 29 Nov 2017 14:41:42 +0000 From: Lester Raff MD To: "Histonet (histonet at lists.utsouthwestern.edu)" Subject: [Histonet] Lab related blog post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF1173C28F at COLOEXCH01.uropartners.local> Content-Type: text/plain; charset="us-ascii" For those interested, Part 1 of a two part blog about pathology and diagnosis. https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.chicagonow.com%2Fdownsize-maybe%2F2017%2F11%2Fi-give-people-cancer-part-1%2F&data=02%7C01%7Cmark.pawlowski%40jefferson.edu%7C1f1f96b2c5c44daf587a08d537531325%7C55a89906c710436bbc444c590cb67c4a%7C0%7C0%7C636475752295278455&sdata=LrIsUOJ4VNFxTjcODqpDsxa7NSDvG9BtSM1%2FinKUZ%2Bg%3D&reserved=0 Have a good week. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ------------------------------ Message: 6 Date: Wed, 29 Nov 2017 17:13:38 +0100 From: "Gudrun Lang" To: 'Julio Benavides Silv?n' Cc: Subject: Re: [Histonet] gross photography Message-ID: <001b01d3692d$04822840$0d8678c0$@gmx.at> Content-Type: text/plain; charset="iso-8859-1" Hi, We use MakroPath from Milestone in a routine histolab. The camera is mounted on the top oft he grossing-station, with an integrated PC+monitor and pedals for zooming and taking photos. Within this system you can mark the pictures, draw something, measure something ... In comparison to the older method with digital-camera, manual zoom etc. it is very conveniant. Picture quality is high. Gudrun -----Urspr?ngliche Nachricht----- Von: Julio Benavides Silv?n via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Dienstag, 28. November 2017 21:27 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] gross photography Hi there, May I ask you your opinion about which system you are using to take gross pictures? We are using a couple of big tungsten light bulbs and a Nikon d60 camera. We are a research lab working with sheep, so we get big lesions in big organs. I was wondering if anybody is using a Digital Gross Photography System and how they compare with a "more ytraditional" digital camera approach. As always, thank you so much for your opinions. Greatly appreciated! Cheers Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cmark.pawlowski%40jefferson.edu%7C1f1f96b2c5c44daf587a08d537531325%7C55a89906c710436bbc444c590cb67c4a%7C0%7C0%7C636475752295278455&sdata=IscLSn7EPmltul9348k2X53IdXsy54jgWu7e8MvupXU%3D&reserved=0 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cmark.pawlowski%40jefferson.edu%7C1f1f96b2c5c44daf587a08d537531325%7C55a89906c710436bbc444c590cb67c4a%7C0%7C0%7C636475752295278455&sdata=IscLSn7EPmltul9348k2X53IdXsy54jgWu7e8MvupXU%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 168, Issue 21 ***************************************** The information contained in this transmission contains privileged and confidential information. It is intended only for the use of the person named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. CAUTION: Intended recipients should NOT use email communication for emergent or urgent health care matters. From Timothy.Morken at ucsf.edu Wed Nov 29 13:19:57 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 29 Nov 2017 19:19:57 +0000 Subject: [Histonet] Beaker with or without Vantage In-Reply-To: References: , Message-ID: Ginny, when you say "slow down our whole process" is that slowness in the system or mismatch of the LIS to the pathology process? My understanding is that this was originally designed for use in public health labs (EpicLab), which are quite different than pathology labs. Now they are trying to adapt a thirty-year old non-pathology system to pathology. Sure would be nice if someone would just use a new pathology-specific LIS built with new technology! Why do we keep having to keep having non-pathology people shove things on us and demand we adapt to their ignorance? We went thru this with Copath for many years and had to teach them how pathology works (they should have been paying us for our knowledge, not charging us to teach them!). Now it seems it is coming around again in a new form. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From: Kurth Virginia L. [mailto:VKurth at uwhealth.org] Sent: Wednesday, November 29, 2017 10:16 AM To: Morken, Timothy; Martha Ward-Pathology Subject: Re: Beaker with or without Vantage Hello We switched to beaker this year, and it definitely had growing pains. I personally believe it was made for a doctor not a lab, and seemed to slow down our whole process, but everyone is going to it. It helps patient care in the aspect of everyone is on the same page with that patient. I think that more and more kinks will be worked out as it continues to adapt to the lab setting. I think asking people when they switched should be considered because it has evolved. Good Luck! Ginny Kurth UW Hospital of Wisconsin ________________________________ From: Martha Ward-Pathology via Histonet > Sent: Tuesday, November 28, 2017 12:30:57 PM To: Morken, Timothy Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Beaker with or without Vantage WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. "They" aren't the ones that will have to use it.....therein lies the rub! I have not heard many positive things about AP Beaker. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward at wakehealth.edu -----Original Message----- From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 28, 2017 11:48 AM To: Histonet Subject: Re: [Histonet] Beaker with or without Vantage I am interested as well. "They" are threatening us with a move to Beaker in the future.... Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Blake Taylor via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 28, 2017 8:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Beaker with or without Vantage I'm looking for anyone out there that has switched to Beaker for AP, Also do you use Ventana Connect with Beaker? Did you choose to use the Beaker tracking system or is anyone using Beaker in conjunction with Vantage? Our Hospital is in the beginning phase of moving from Copath Sunquest to Beaker (2017 version) . Any thoughts of what has gone well and what has not would be appreciated. Thanks so much Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes at lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use to the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Blanca.Lopez at UTSouthwestern.edu Wed Nov 29 16:14:20 2017 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Wed, 29 Nov 2017 22:14:20 +0000 Subject: [Histonet] agar to make cell blocks Message-ID: <916fad5012084a2bbc6c31777602a185@SWMS13MAIL12.swmed.org> Hi everybody! Is anybody willing to share their protocols to make a cell block please. I have a customer that use agarose 2% (nusieve GTG agar) but we are having trouble to take the gel out of the tube, breaks easily even when I try to cut the gel cylinder. I told her to make agar 4% microwave little to make it liquid transfer some with a pipette, stir and centrifuge for 10 min 2000 rpm. Which agar do you recommends for cell? Or any other recommendation to improve the technique? Do you know if there is a differences between brands? Please give your opinion. thanks ________________________________ UT Southwestern Medical Center The future of medicine, today. From cls71877 at gmail.com Wed Nov 29 17:35:05 2017 From: cls71877 at gmail.com (Cristi Rigazio) Date: Wed, 29 Nov 2017 18:35:05 -0500 Subject: [Histonet] Beaker with or without Vantage In-Reply-To: References: Message-ID: <94D8C73F-C216-4A6A-B484-1EF21D985F44@gmail.com> We use Beaker across our system and like anything it has its pros and cons. Tim is correct, it was NOT developed for Pathology, rather for physicians and the lab module was an afterthought. Our IHC lab uses Vantage with Middleware and things go really well for them to my knowledge, but it certainly wasn?t without its challenges. Changing any system is going to come with growing pains, for sure, but some of the things I would want ?redone? include tracking, as a multiple site system we have issues with packing lists moving tests with materials to other labs and keeping the pathologists from signing cases out. We lose our tracking from the gross station and pick it back up at embedding, using manual batch tracking sheets for what goes on the processors. I believe something has been built to help with this and we use a wingman system, so others might not have this issue. From a manager perspective, The report system is getting a little insane as with upgrades there seems to be unforeseen changes that impact reports you may rely on, also there are a variety of ways to develop or run reports (workbench reports, Clarity and Universe!) and the redundancy is getting confusing. From the bench perspective, depending on the type of lab you are, accessioning is accomplished three different ways for us, client specimens that we have to register, within system specimens that are ordered in a manner that we have to go ?grab? the order and the ideal way where we simply scan the label and input the task protocol (again within the system, but why can the doctors/nurses use two different ways to input the order??). Scanning the barcodes from the cassettes can be an issue, but that is not Beakers fault, make sure you have solid cassette printers. Lastly, the physicians view in Epic is much different than your view in Beaker, this has caused confusion when trying to find things like additional testing reports performed outside and then scanned in. We have hammered through the majority of these issues and the Beaker team is great at helping out and thinking outside the box to smooth out workflows. Oh, and billing! Make sure you test all the way through patient accounting! Stay positive, as noted, likely the choice isn?t yours to make, but how you approach it is. Build a good relationship with your Beaker team, prioritize your needs and chip away. It will work out. Sent from my iPhone > On Nov 29, 2017, at 2:19 PM, Morken, Timothy via Histonet wrote: > > Ginny, when you say "slow down our whole process" is that slowness in the system or mismatch of the LIS to the pathology process? > > My understanding is that this was originally designed for use in public health labs (EpicLab), which are quite different than pathology labs. Now they are trying to adapt a thirty-year old non-pathology system to pathology. Sure would be nice if someone would just use a new pathology-specific LIS built with new technology! Why do we keep having to keep having non-pathology people shove things on us and demand we adapt to their ignorance? We went thru this with Copath for many years and had to teach them how pathology works (they should have been paying us for our knowledge, not charging us to teach them!). Now it seems it is coming around again in a new form. > > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > From: Kurth Virginia L. [mailto:VKurth at uwhealth.org] > Sent: Wednesday, November 29, 2017 10:16 AM > To: Morken, Timothy; Martha Ward-Pathology > Subject: Re: Beaker with or without Vantage > > > Hello > > We switched to beaker this year, and it definitely had growing pains. > > I personally believe it was made for a doctor not a lab, and seemed to slow down our whole process, > > but everyone is going to it. It helps patient care in the aspect of everyone is on the same page with that > > patient. I think that more and more kinks will be worked out as it continues to adapt to the lab setting. I think > > asking people when they switched should be considered because it has evolved. Good Luck! > > > > > > Ginny Kurth > > > UW Hospital of Wisconsin > > ________________________________ > From: Martha Ward-Pathology via Histonet > > Sent: Tuesday, November 28, 2017 12:30:57 PM > To: Morken, Timothy > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Beaker with or without Vantage > > WARNING: This email appears to have originated outside of the UW Health email system. > DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. > > > > > "They" aren't the ones that will have to use it.....therein lies the rub! I have not heard many positive things about AP Beaker. > > > > Martha Ward, MT (ASCP) QIHC > Manager > > Molecular Diagnostics Lab > Medical Center Boulevard \ Winston-Salem, NC 27157 > p 336.716.2109 \ f 336.716.5890 > mward at wakehealth.edu > > > > > > -----Original Message----- > From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, November 28, 2017 11:48 AM > To: Histonet > Subject: Re: [Histonet] Beaker with or without Vantage > > I am interested as well. "They" are threatening us with a move to Beaker in the future.... > > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center > > -----Original Message----- > From: Blake Taylor via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, November 28, 2017 8:10 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Beaker with or without Vantage > > I'm looking for anyone out there that has switched to Beaker for AP, Also do you use Ventana Connect with Beaker? Did you choose to use the Beaker tracking system or is anyone using Beaker in conjunction with Vantage? Our Hospital is in the beginning phase of moving from Copath Sunquest to Beaker (2017 version) . Any thoughts of what has gone well and what has not would be appreciated. > > Thanks so much > > Blake Taylor > Surgical Pathology Supervisor > Lexington Medical Center > 803-936-8214 > bcdukes at lexhealth.org > > PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use to the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff at uropartners.com Thu Nov 30 07:02:55 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 30 Nov 2017 13:02:55 +0000 Subject: [Histonet] Pathologist Blog Part 2 Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF1173CB50@COLOEXCH01.uropartners.local> For those of you who read Part 1 (or even if you didn't) here is part 2. http://www.chicagonow.com/downsize-maybe/2017/11/i-give-people-cancer-part-2/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From relia1 at earthlink.net Thu Nov 30 10:17:22 2017 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 30 Nov 2017 11:17:22 -0500 Subject: [Histonet] RELIA Histology Careers Bulletin 11-30-2017 I hope you had a Wonderful Thanksgiving!!!! Message-ID: <00ae01d369f6$b4291960$1c7b4c20$@earthlink.net> Hi Histonetters! I hope you had a wonderful Thanksgiving and got your fill of turkey and stuffing and pie and all of the good stuff!! I know I did!! In my last postl I promised some exciting new opportunities Sooo. Before we dive head first into the rest of the holiday season I wanted to give you a heads up on the new and exciting positions I am working on. If you are considering a job change after the first of the year it might not be a bad idea for me to start working on it now on your behalf. We can certainly postpone onsite interviews and decisions until January but let's get the jump on things. That way after the New Year when EVERYBODY else starts sending their resumes out we will be ahead of the game!! You would be surprised at how many great opportunities I have landed for people with this strategy because so many jobseekers and recruiters are "asleep at the wheel" during the month of December!! So do me this favor and grab a mug of cocoa and take a quick look at the listings. Here is a list of my current openings: HISTOLOGY OPPORTUNITIES: Histology Supervisor - Birmingham, AL IHC/Molecular Product Manager - San Francisco Histotech - San Diego, CA Histotech - Austin, TX Histotech - Birmingham, AL IHC Specialist - Boston, MA area Most of these opportunities are RELIA EXCLUSIVES!! All of these are permanent full time positions and my clients offer excellent compensation and benefits. Relocation assistance and/or sign on bonuses If something looks interesting shoot me an email and let me know. If you are looking and nothing on the list strikes your fancy then shoot me an email and tell me where you want to go so I can keep an eye out for things in that area. And if you are totally happy where you are keep an eye out for people who are looking after all wouldn't a referral fee help with your Christmas shopping bills in 2018? Happy Holidays !!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From KSimeone at leavittmgt.com Thu Nov 30 17:42:30 2017 From: KSimeone at leavittmgt.com (Kari Simeone) Date: Thu, 30 Nov 2017 23:42:30 +0000 Subject: [Histonet] FT HISTOTECH POSITION DELRAY BEACH FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CF11E1462@vm-email.leavittmgt.com> Hi Histonetters! We are looking for TWO full time licensed HISTOTECHNOLOGISTS here in our very busy Delray Beach Florida Dermatology Lab. These are permanent full time (40 hours) positions with benefits (medical/401k/vacation). THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Please fill out employment application HERE https://www.indeed.com/m/viewjob?jk=bb1a5018e0e69496&from=serp&prevUrl=https%3A%2F%2Fwww.indeed.com%2Fm%2Fjobs%3Fq%3Dhistotechnologist%26l%3DDelray%2BBeach%252C%2BFL%26from%3Dhome%252Cwhereauto%252Cwhatauto ^^you MUST follow this application link to apply! No exceptions. *FULL TIME position Mon-Fri OR Sun-Thurs 5P-1:30AM (START TIME CAN VARY FROM 5-10P) *FULL TIME position Mon-Fri 11a-7:30p (GROSSING/EMBEDDING) *MUST be licensed as a FL HISTOTECHNOLOGIST/HISTOTECHNICIAN ONLY *MUST have at LEAST FIVE (5) years experience (dermatology preferred) for EVENING SHIFT Please DO NOT respond if no EXPERIENCE! *VERY proficient in embedding and microtomy *WILL MOSTLY BE EMBEDDING EXCISION BLOCKS, please know DERMS (NIGHT SHIFT) *must be self motivated, reliable and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) *some IHC experience preferred but not necessary Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. 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