[Histonet] IHC testing for Parvovirus (Human)

[Tony Henwood (SCHN)]

Hi Richard,
We use NCL-PARVO from Leica

Details as follows:

ANTIGEN NAME:        Parvovirus B19
OTHER NAMES:         

Clone:	    R92F6	 
Isotype:  	IgG1  
		

DESCRIPTION:      Mouse monoclonal to native parvovirus B19 purified from human plasma. Human parvovirus B19 (VP1 and VP2 capsid proteins).
          	
STORAGE CONDITIONS:        4-8oC undiluted

SUPPLIER:            Leica  NCL-PARVO
PROCEDURE:

Methodology:        HRP-POLYMER Working Dilution:     		Bond:	  1/100
Special Conditions:      Citrate HIER required (ER1) 
CONTROL TISSUE:     Placenta containing Parvovirus	 

Inbuilt Controls 	 	 
CLINICAL SIGNIFICANCE:
Diagnosis of parvovirus B19 infection in formalin-fixed, paraffin embedded tissue can be made through the identification of the characteristic brick-red intranuclear inclusions in normoblasts within the fetal circulation in histological sections stained with HE. It has been found that immunohistochemistry was the best detection method. It is highly specific and sensitive, preserves the morphology and reveals a larger number of positive cells than does HE with the advantage of showing cytoplasmic and nuclear positivity, making it more reliable.
REFERENCES:
Li, J. J., Henwood, T., Van Hal, S., & Charlton, A. (2015). Parvovirus infection: an immunohistochemical study using fetal and placental tissue. Pediatric and Developmental Pathology, 18(1), 30-39.
Quemelo PRV, Lima DM, Fonseca BAL, Peres LC (2007) "Detection of parvovirus B19 infection in formalin-fixed and paraffin-embedded placenta and fetal tissues" Rev. Inst. Med. trop. S. Paulo, 49(2): 103-107,

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, 24 May 2017 4:33 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] IHC testing for Parvovirus (Human)

If you do this test, where do you get your antibody?  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


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