From mpence at grhs.net Mon May 1 08:55:04 2017 From: mpence at grhs.net (Mike Pence) Date: Mon, 1 May 2017 13:55:04 +0000 Subject: [Histonet] Her2 Dual ISH Message-ID: What CPT code is everyone that does Her2 Dual ISH use? There are a lot of codes for ISH to choose from. Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 mpence at grhs.net | www.greatrivermedical.org. www.Facebook.com/GreatRiverHealthSystems | www.Twitter/GreatRiverMed Information in this communication, including attachments, is confidential and intended only for the addressee(s). This communication may contain privileged, confidential, proprietary or trade secret information entitled to protection or exemption from disclosure under law. If you are not an intended recipient, please know that any use, distribution or copying of this communication, or any action taken based on the information in this communication, is unauthorized and may be unlawful. If you received this communication in error, please notify the sender and delete this communication from your device. From amurvosh at advancederm.net Mon May 1 11:07:57 2017 From: amurvosh at advancederm.net (Anne Murvosh) Date: Mon, 1 May 2017 16:07:57 +0000 Subject: [Histonet] Slide disposal Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7B51AF3@Exchange.Advancederm.net> When disposing of slides after the 10 period is up, what is everyone doing to protect patient confidentiality? In the old days we would just put packing tape around the box and toss. With patient names on the slides and sometimes specimen location on the new labels I'm wondering how to dispose of them properly. Thanks Anne From fpearsa at clemson.edu Mon May 1 12:18:27 2017 From: fpearsa at clemson.edu (Frances Pearsall) Date: Mon, 1 May 2017 17:18:27 +0000 Subject: [Histonet] Gum Mastic/ Steiner Message-ID: I have a question for Labs that use the Steiner Stain. What do you store your Gum Mastic in inside your refrigerators to satisfy flammable storage requirements? Thank you for any suggestions, Fran From sandra.cheasty at wisc.edu Tue May 2 09:10:05 2017 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Tue, 02 May 2017 14:10:05 +0000 Subject: [Histonet] Refurbished Leica RM2135 Message-ID: Hi everyone, I have a newly refurbished Leica RM2135 at my disposal, but the knife holder is for high profile blades. 2 questions. 1-Is it that different using high-profile blades vs. low. (I have used low-profile blades forever.) 2-There is a slot on the bottom of the blade holder, and I seem to remember that it can be converted to a low-profile holder by sliding an narrow metal strip into that slot. I know I've done this before, but it was long ago, and the used equipment guy says there's no such thing. Thank you! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From owensc500 at gmail.com Tue May 2 09:23:59 2017 From: owensc500 at gmail.com (Clarence Owens) Date: Tue, 2 May 2017 10:23:59 -0400 Subject: [Histonet] AFB Control Blocks Message-ID: Good Morning All, I am in search for 2 blocks of AFB positive lung blocks. Does anyone have 2 blocks they could spare. I am willing to trade a control block for another control block. I thank you for your help in advance. Regards, - Clarence Owens ?, HT (ASCP) QIHC? From maxim_71 at mail.ru Tue May 2 13:12:10 2017 From: maxim_71 at mail.ru (=?UTF-8?B?0J/QtdGI0LrQvtCyINCc0LDQutGB0LjQvA==?=) Date: Tue, 02 May 2017 21:12:10 +0300 Subject: [Histonet] =?utf-8?q?Refurbished_Leica_RM2135?= Message-ID: <1493748730.425887489@f54.i.mail.ru> Sandra, high profile blades was better for me, because they are harder and more durable than low profile of any manufacturer. Also I recommend for you to try Surgipath DB80HS. They must better than 818.?I regret that not having bought a holder for a high profile blades. Leica makes the blade holders only for one type blades (low or high). Microm's blade holder made for both type blades. -- Sincerlely, Maxim Pehkov Russia, Taganrog. From maxim_71 at mail.ru Tue May 2 13:38:55 2017 From: maxim_71 at mail.ru (=?UTF-8?B?0J/QtdGI0LrQvtCyINCc0LDQutGB0LjQvA==?=) Date: Tue, 02 May 2017 21:38:55 +0300 Subject: [Histonet] =?utf-8?q?Refurbished_Leica_RM2135?= In-Reply-To: References: <1493748730.425887489@f54.i.mail.ru> Message-ID: <1493750335.316460955@f312.i.mail.ru> From sugh expensive blades can be done more very good sections (at 3 microns) from any difficult type tissue. These investments will pay off very quickly. Maxim Peshkov Russia, Taganrog. >???????, 2 ??? 2017, 21:16 +03:00 ?? "Howery, Jeff L" : > >But, high profile knives are more expensive. This is due to the increased amount of Stainless steel needed to make them. > >-----Original Message----- >From: ?????? ?????? via Histonet [mailto:histonet at lists.utsouthwestern.edu] >Sent: Tuesday, May 02, 2017 11:12 AM >To: Sandra Cheasty >Cc: histonet at lists.utsouthwestern.edu >Subject: Re: [Histonet] Refurbished Leica RM2135 > > >Sandra, high profile blades was better for me, because they are harder and more durable than low profile of any manufacturer. Also I recommend for you to try Surgipath DB80HS. They must better than 818.?I regret that not having bought a holder for a high profile blades. >Leica makes the blade holders only for one type blades (low or high). >Microm's blade holder made for both type blades. >-- >Sincerlely, >Maxim Pehkov >Russia, >Taganrog. > > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer at mdanderson.org Tue May 2 14:23:38 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Tue, 2 May 2017 19:23:38 +0000 Subject: [Histonet] . Refurbished Leica RM2135 Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8839ED9F06@D1PWPEXMBX08.mdanderson.edu> Sandra, On some holders you can easily convert back and forth from low to high. If your service person does not know anything about that, I would ask Leica. Specifically ask for a tech rep with the most microtome experience (in years). That person should be able to tell you what part you need. They may even have an old one lying around for you. Also check with some of the more established labs in your area, they may have a blade holder that you can get for parts. Toysha Mayer Message: 2 Date: Tue, 02 May 2017 14:10:05 +0000 From: Sandra Cheasty To: "Histonet (histonet at lists.utsouthwestern.edu)" Subject: [Histonet] Refurbished Leica RM2135 Message-ID: Content-Type: text/plain; CHARSET=US-ASCII Hi everyone, I have a newly refurbished Leica RM2135 at my disposal, but the knife holder is for high profile blades. 2 questions. 1-Is it that different using high-profile blades vs. low. (I have used low-profile blades forever.) 2-There is a slot on the bottom of the blade holder, and I seem to remember that it can be converted to a low-profile holder by sliding an narrow metal strip into that slot. I know I've done this before, but it was long ago, and the used equipment guy says there's no such thing. Thank you! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From tratroyer at gmail.com Tue May 2 16:15:47 2017 From: tratroyer at gmail.com (Travis Troyer) Date: Tue, 2 May 2017 16:15:47 -0500 Subject: [Histonet] Cryostat and Parts Message-ID: We are in the process of moving and we have some equipment and supplies that we are not taking with us. We have 2 Microm HM 505E cryostats and all of the internal components. One of the cryostats doesn't cool down and the other one doesn't advance properly but is otherwise in working order. If anyone is interested in the cryostats or the any of the parts please let me know. We also have 12 bottles of Polarstat Plus cryostat mounting media (exp 6/18). If anyone is interested please let me know. Travis Troyer Advanced Dermatology and Skin Cancer Center 1133 College Ave., Suite E230 Manhattan, KS 66502 Tel: 785-537-4990 <(785)%20537-4990> Fax: 785-537-1938 <(785)%20537-1938> derm at ksderm.com From ian.bernard at comcast.net Tue May 2 22:18:23 2017 From: ian.bernard at comcast.net (ian bernard) Date: Tue, 2 May 2017 21:18:23 -0600 Subject: [Histonet] Gold Chloride Source Message-ID: <005c01d2c3bb$ec57bf80$c5073e80$@comcast.net> Histonetters, looking for Gold Chloride purchase source. Current distributor has the product on back order- Sigma Aldrich. Need another source since we are running low. Please provide catalog number as well. IB From Ana.Maluenda at baker.edu.au Tue May 2 22:56:07 2017 From: Ana.Maluenda at baker.edu.au (Ana Maluenda) Date: Wed, 3 May 2017 03:56:07 +0000 Subject: [Histonet] H&E on frozen sections Message-ID: Hi everyone, Just wondering if anyone out there had already had issues with commercial Haematoxylin and Eosin. I started working in a lab that recently purchased Sigma Mayer's Haematoxylin (MHS-16) and 1% Alcoholic Eosin (Fronine - II016). We work with fresh frozen sections of mouse tissue. Since then, H&E protocol haven't worked well. I tried different protocols, following the product sheet as well as following other researcher's protocols and none worked. Both Haematoxylin and Eosin are very faint, with Eosin been uptake very inconsistently even on the same section (some of the areas are not even stained at all). I also used sections from different projects, with different people performing tissue harvest, OCT embedding and sectioning (so it looks more a problem with the staining protocol rather than the specimens) and tried increasing the staining times. Any ideas would be much appreciated. Also, what are people using as commercial staining Haem and Eosin (brand and catalogue number)? Thanks very much! Kind regards, Ana Ana Maluenda Research Assistant Atherothrombosis and Vascular Biology Laboratory Baker Heart and Diabetes Institute 75 Commercial Road, Melbourne VIC 3004 P (03) 8532 1359 E Ana.Maluenda at baker.edu.au W www.baker.edu.au Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting privacy at baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg From sgkcck at aol.com Wed May 3 03:15:56 2017 From: sgkcck at aol.com (Sgkcck) Date: Wed, 3 May 2017 04:15:56 -0400 Subject: [Histonet] Gold Chloride Source In-Reply-To: <005c01d2c3bb$ec57bf80$c5073e80$@comcast.net> References: <005c01d2c3bb$ec57bf80$c5073e80$@comcast.net> Message-ID: <15bcd6147a2-6037-1a954@webprd-m45.mail.aol.com> Good day. We have and produce Gold Chloride on a dialy basis and there is no stock issue Thank you and we look forward to hearing from you Stacie Kirsch Electron Microscopy Sciences 1560 Industry Road Hatfield Pa 19440 Tel: 215-412-8400 Fax: 215-412-8450 www.emsdiasum.com -----Original Message----- From: ian bernard via Histonet To: histonet Sent: Tue, May 2, 2017 11:32 pm Subject: [Histonet] Gold Chloride Source Histonetters, looking for Gold Chloride purchase source. Current distributor has the product on back order- Sigma Aldrich. Need another source since we are running low. Please provide catalog number as well. IB _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From taylor at prometheushealthcare.com Wed May 3 13:44:23 2017 From: taylor at prometheushealthcare.com (Taylor Rinaldi) Date: Wed, 3 May 2017 14:44:23 -0400 Subject: [Histonet] NEW Histology role in South Florida! Message-ID: <077e01d2c43d$48d937d0$da8ba770$@prometheushealthcare.com> Hi Histonetters! My name is Taylor Rinaldi, I'm the Recruiting Manager with Prometheus Healthcare. My team and I are currently recruiting for a Dermatology office in South Florida. They are looking for a FL licensed HT candidate to perform Mohs, frozen sections and permanent process, and someone who can also be responsible for administrative aspects such as maintaining logs, equipment and P&P manuals. This position is a full time and permanent opportunity, just minutes from the beach! The position is Monday- Friday between 7:30 am- 5 pm. If you may be interested in learning more, please reach out to me for immediate consideration! Thank you all in advance! Taylor Rinaldi Recruiting Manager Prometheus Healthcare Office 866-857-1434 Taylor at prometheushealthcare.com From taylor at prometheushealthcare.com Wed May 3 13:58:05 2017 From: taylor at prometheushealthcare.com (Taylor Rinaldi) Date: Wed, 3 May 2017 14:58:05 -0400 Subject: [Histonet] NEW Histology roles in NYC! Message-ID: <079101d2c43f$32efdd50$98cf97f0$@prometheushealthcare.com> Hi Histonetters! My name is Taylor Rinaldi, I'm the Recruiting Manager with Prometheus Healthcare. My team and I are always recruiting for different laboratories in the New York City area and we're always looking for new talent! Currently, we have one position in Manhattan available, working with a large Neurological Laboratory. This position is a day shift, Monday - Friday, full time and permanent. All of our other ongoing HT roles in New York do require a NY state license. Previous experience is preferred although entry level would be considered as well. Sign on Bonus potential offered, pending each laboratory and location. If you may be interested in learning more, please reach out to me for immediate consideration. We may be working directly with a company you have been hoping to join, so don't hesitate to reach out to get a conversation going about furthering your HT career. We are here to help! The best decision is an informed decision! Thank you all in advance, Taylor Rinaldi Recruiting Manager Prometheus Healthcare Office 866-857-1434 Taylor at prometheushealthcare.com From jriggleman at globusmedical.com Wed May 3 17:36:55 2017 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Wed, 3 May 2017 22:36:55 +0000 Subject: [Histonet] Ultrasonic Cleaning Bath Message-ID: Hello, I am looking for a used ultrasonic cleaning bath and a used plate shaker. If you have either one laying around your lab please let me know! Will pay. Thank You, Jessica Riggleman _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From Karoleigh.Armstrong at ARMC.net Thu May 4 08:30:11 2017 From: Karoleigh.Armstrong at ARMC.net (Armstrong, Karoleigh T) Date: Thu, 4 May 2017 13:30:11 +0000 Subject: [Histonet] Gold Chloride Message-ID: We buy from American Master Tech. -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From thisisann at aol.com Thu May 4 15:08:54 2017 From: thisisann at aol.com (Ann Specian) Date: Thu, 4 May 2017 16:08:54 -0400 Subject: [Histonet] Acid Hematoyxlin stain for Urine Message-ID: <15bd51462d7-1c21-1bd5f@webprd-a98.mail.aol.com> Does anyone have a procedure for an acid hematoxylin stain for urine cytology? I believe Bostwick used to/does perform it...... Ann From tkngflght at yahoo.com Thu May 4 19:41:03 2017 From: tkngflght at yahoo.com (Cheryl) Date: Fri, 5 May 2017 00:41:03 +0000 (UTC) Subject: [Histonet] Histology Lead position - help! References: <795648491.3389545.1493944863649.ref@mail.yahoo.com> Message-ID: <795648491.3389545.1493944863649@mail.yahoo.com> Houston, we have a problem! We need a Histologist Lead tech. ?My acting lead is leaving us to take care of aging family in another state (love that she can make this choice). ?This leaves us with a hole in our staff that really needs filled up fast! Have to be gross qualified-- we can teach and it's mostly derm and GI -- you do need to have the base education.? And registered--either HT or HTL. ? It's not a complicated lab-- and we like each other and have fun most days. ? Our new facilities will be ready in August. South Houston-- and affordable place to live. I'll answer your emails-- please have a resume for me. ?ckerry at adgpath.com THANKS!! Cheryl Kerry, HT(ASCP)ckerry at adgpath.com From JMacDonald at mtsac.edu Thu May 4 19:55:27 2017 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Thu, 4 May 2017 17:55:27 -0700 Subject: [Histonet] California Society for Histotechnology Message-ID: It's not too late to register for the 2017 CSH 40th Annual symposium. Lots of great workshops! No late fees! http://californiahistology.org/events.html#csh2017 From Richard.Cartun at hhchealth.org Fri May 5 11:11:02 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 5 May 2017 16:11:02 +0000 Subject: [Histonet] Pathology Slides Send-Outs Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E9541BE8A@HHCEXCHMB03.hhcsystem.org> I'm wondering if any of you take a patient's credit card to pay for the FedEx shipping of pathology slides when they request that their slides be sent to another hospital or medical institution? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From LRaff at uropartners.com Fri May 5 11:51:29 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 5 May 2017 16:51:29 +0000 Subject: [Histonet] Pathology Slides Send-Outs In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E9541BE8A@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E9541BE8A@HHCEXCHMB03.hhcsystem.org> Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF113A776D@COLOEXCH01.uropartners.local> We have a policy that says we do, but I find my clerical staff frequently waives the fee. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, May 05, 2017 11:11 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pathology Slides Send-Outs I'm wondering if any of you take a patient's credit card to pay for the FedEx shipping of pathology slides when they request that their slides be sent to another hospital or medical institution? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at dpspa.com Mon May 8 08:28:27 2017 From: criley at dpspa.com (Charles Riley) Date: Mon, 8 May 2017 09:28:27 -0400 Subject: [Histonet] Film coverslipping Message-ID: Does anyone know if film coverslipping with a sakura coverslipper is validated and able to be used with Halogic imaging system? Looking to purchase a new coverslipper but want the slides to be compatible with the imager. Any help is appreciated -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From redrose297 at gmail.com Mon May 8 11:00:47 2017 From: redrose297 at gmail.com (warda hassan) Date: Mon, 08 May 2017 16:00:47 +0000 Subject: [Histonet] Microscope selection Message-ID: Hello to all Would like to have some advice from expert from the field of pathology on microscope selection Which one would you select as a manufacturer for microscopes 1- Olympus 2- Nikon 3- Leica Thank you Good day From jwwalker at rrmc.org Mon May 8 11:15:53 2017 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Mon, 8 May 2017 16:15:53 +0000 Subject: [Histonet] Microscope selection In-Reply-To: References: Message-ID: I've used a variety of microscopes in my 20 years in the cytology field. At this point, I believe that the optics between Olympus and Nikon are pretty much equal. I can't comment on Leica as the last one of these that I used was over 20 years old and not worth the comparison. In a laboratory setting, we found that the mechanical components like stages and objective nose rings lasted longer with the Olympus scopes. I don't believe that you can go wrong with either choice but it probably comes down to personal preferences of the user and comparison of some of the "options" that each offers (telescoping heads, tilting heads, ergonomic design, adjustments, etc.) For us a driving factor was the ease of a digital camera and the software involved with the capture of photomicrographs. Olympus was easier to operate by a small margin. I am sure that your local vendor would be willing to setup demos of their models within your lab for staff to try. I would caution that you ask about the objectives on the demo scopes. I have found that a vendor will often put their highest quality objectives on their demos and not necessarily the objectives that you will see quoted. These objectives are very costly and may not be needed in all areas of the lab. I should state that I do not own stock in either company and the views are solely my own based on multiple lab experiences. Cheers, Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewalker at rrmc.org, www.rrmc.org Our Vision: To Be the Best Community Healthcare System in New England Our Commitment to our Community: We Listen, We Respect, We Care . . . Always. Joint Commission Accredited | Leapfrog Hospital Safety A Rating, Spring | ANCC Magnet Hospital Designation(r) Healthgrades: Excellence Award for Patient Safety | Healthgrades: Outstanding Patient Experience Award -----Original Message----- From: warda hassan via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 08, 2017 12:01 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Microscope selection Hello to all Would like to have some advice from expert from the field of pathology on microscope selection Which one would you select as a manufacturer for microscopes 1- Olympus 2- Nikon 3- Leica Thank you Good day _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ajayiifeoluwa at gmail.com Mon May 8 11:34:01 2017 From: ajayiifeoluwa at gmail.com (Ifeoluwa Ajayi) Date: Mon, 8 May 2017 17:34:01 +0100 Subject: [Histonet] Microscope selection In-Reply-To: References: Message-ID: Dear Hassan, Olympus has been my companion for some years now, so for me it is Olympus and only Olympus. Ajayi Ifeoluwa BMLS, AMLSCN, MSc. University College Hospital, Ibadan, Nigeria. On May 8, 2017 5:11 PM, "warda hassan via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > Hello to all > Would like to have some advice from expert from the field of pathology on > microscope selection > Which one would you select as a manufacturer for microscopes > > 1- Olympus > 2- Nikon > 3- Leica > > Thank you > Good day > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rsrichmond at gmail.com Mon May 8 13:04:45 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Mon, 8 May 2017 14:04:45 -0400 Subject: [Histonet] Microscope selection Message-ID: Warda Hassan (where?) >>would like to have some advice from expert from the field of pathology on microscope selection. Which one would you select as a manufacturer for microscopes?<< Speaking from more than 50 years of going to and fro in the world of pathology, and walking up and down in it (apologies to Job 1:7b KJV): A pathologist gets one new microscope per career, and if it was an AO student scope 40 years ago (literally, I've had to work with several of them), SOL. The lab manager selects and specifies the pathologist's microscope, without input from the pathologist, and the pathologist needs to remember to put the dust cover on the microscope every evening. More detailed specs may be found in the annual update of The Hospital Administrator and Lab Manager's Handy-Dandy Guide to Making Life Hard for the Pathologist (ASCP Press publication). Some specific items: 2x low power objective lens: fuggeddabbouddit 100x oil immersion lens: ditto optical micrometer: ditto double head microscope: ditto polarizer: there's one on the pee scope across the hall photomicrography: jiss use your iPhone whole slide imaging: what's that? Bob Richmond Samurai Pathologist Maryville TN From abtdhu at gmail.com Mon May 8 13:16:09 2017 From: abtdhu at gmail.com (Dorothy Hu) Date: Mon, 8 May 2017 14:16:09 -0400 Subject: [Histonet] KOH softener to "decalcify" bone? Message-ID: I know someone used KOH with EDTA together to decalcify bone. It is worthwhile to try KOH only to soft bone and kept dynamic labeling. If anyone had tried successfully, please let us know. Thanks. > > Today's Topics: > > 1. Fabric softener to "decalcify" bone? (Angela Lamberth) > 2. Re: Fabric softener to "decalcify" bone? - Downy has formic > acid?? (Angela Lamberth) > > > From jwwalker at rrmc.org Mon May 8 13:59:08 2017 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Mon, 8 May 2017 18:59:08 +0000 Subject: [Histonet] Film coverslipping In-Reply-To: References: Message-ID: The Hologic Imaging system has been approved for both glass and ribbon coverslips. I'd suggest you reach out to your local Hologic rep for any specifics that you might have. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewalker at rrmc.org, www.rrmc.org Our Vision: To Be the Best Community Healthcare System in New England Our Commitment to our Community: We Listen, We Respect, We Care . . . Always. Joint Commission Accredited | Leapfrog Hospital Safety A Rating, Spring | ANCC Magnet Hospital Designation(r) Healthgrades: Excellence Award for Patient Safety | Healthgrades: Outstanding Patient Experience Award -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 08, 2017 9:28 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Film coverslipping Does anyone know if film coverslipping with a sakura coverslipper is validated and able to be used with Halogic imaging system? Looking to purchase a new coverslipper but want the slides to be compatible with the imager. Any help is appreciated -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SteveM at mcclainlab.com Tue May 9 06:31:00 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Tue, 9 May 2017 11:31:00 +0000 Subject: [Histonet] Histonet Digest, Vol 162, Issue 6 In-Reply-To: References: Message-ID: Olympus microscopes win in my experience. You can't go wrong with any of the 3 major manufacturers. Modern scopes all have have better optics than what we trained on. I place a high priority on durability and in my experience with Nikon and Olympus, Olympus has consistently shown better overall quality and durability and wins hands down. Why? In my personal experience, in scopes getting used 8-10 hours a day, and multiple users, Olympus survives better in terms of rubber parts such as ocular cups and the ring around the objective turret which failed on both Nikons. In other words, where the rubber meets the road ( or the pathologist touching the rubber), both Nikons suffered. During my first 10 years, I used Nikon scopes exclusively, and I still own one Nikon scope (albeit stored in a cabinet). After 2-4 years both stages wore out and those rubber parts needed replacing. I purchased 3 different digital cameras in my first 5 years of slide imaging (Polaroid and Spot) and 2 different Nikon scopes 400 and e600. But in my own private lab experience Olympus is clearly more durable. I wore out 1 stage and wore out 1 motorized microscope head in 14 years of rigorous daily use since changing to Olympus in 2004. That's it. 3 pathologists 3 scopes, 100,000 slides a year. 2 repairs* We rely on our microscopes to work to a greater degree than most labs because we IMAGE EVERY SPECIMEN. Having a scope down or having suboptimal performance inevitably shows up in the images. Olympus scopes BX 61 BX41 with digital cameras DP70 DP71 have held up really well. 3 student grade scopes in the dirty lab environ at each microtome cutting station, used for immediate wet slide inspection and KOH exams, have held up well. Perhaps most surprisingly, both Olympus digital camera models (surprisingly because they are now 12-14 years old) still produce great slide images and remain in daily use. The scopes likewise. If you are buying a scope to be replaced every 3 years, you may see little difference. But if you plan to use for 3-10 years with little maintenance aside from cleaning, odds favor Olympus. Steve A. McClain, MD *PS truth be told I also left the UV lamp on for a week 240 hours and burned up the UV lamp housing and had to replace that. Adding of course a $12 darkroom timer to prevent that accident from repeating. On May 8, 2017, at 13:09, "histonet-request at lists.utsouthwestern.edu" > wrote: Subject: [Histonet] Microscope selection Message-ID: > Content-Type: text/plain; charset=UTF-8 Hello to all Would like to have some advice from expert from the field of pathology on microscope selection Which one would you select as a manufacturer for microscopes 1- Olympus 2- Nikon 3- Leica From SteveM at mcclainlab.com Tue May 9 06:33:06 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Tue, 9 May 2017 11:33:06 +0000 Subject: [Histonet] Fwd: Histonet Digest, Vol 162, Issue 6 micrcroscopes In-Reply-To: References: , Message-ID: <2F7CE9F1-7FE4-4415-8455-D65377AA16B4@mcclainlab.com> Olympus microscopes win in my experience. You can't go wrong with any of the 3 major manufacturers. Modern scopes all have have better optics than what we trained on. I place a high priority on durability and in my experience with Nikon and Olympus, Olympus has consistently shown better overall quality and durability and wins hands down. Why? In my personal experience, in scopes getting used 8-10 hours a day, and multiple users, Olympus survives better in terms of rubber parts such as ocular cups and the ring around the objective turret which failed on both Nikons. In other words, where the rubber meets the road ( or the pathologist touching the rubber), both Nikons suffered. During my first 10 years, I used Nikon scopes exclusively, and I still own one Nikon scope (albeit in storage). After 2-4 years both stages wore out and those rubber parts needed replacing. I purchased 3 different digital cameras in my first 5 years of slide imaging (Polaroid and Spot) and 2 different Nikon scopes 400 and e600. But in my own private lab experience Olympus is clearly more durable. I wore out 1 stage and wore out 1 motorized microscope head in 14 years of rigorous daily use since changing to Olympus in 2004. That's it. 3 pathologists 3 scopes, 100,000 slides a year. 2 repairs* We rely on our microscopes to work to a greater degree than most labs because we IMAGE EVERY SPECIMEN. Having a scope down or having suboptimal performance inevitably shows up in the images. Olympus scopes BX 61 BX41 with digital cameras DP70 DP71 have held up really well. 3 student grade scopes in the dirty lab environ at each microtome cutting station, used for immediate wet slide inspection and KOH exams, have held up well. Perhaps most surprisingly, both Olympus digital camera models (surprisingly because they are now 12-14 years old) still produce great slide images and remain in daily use. The scopes likewise. If you are buying a scope to be replaced every 3 years, you may see little difference. But if you plan to use for 3-10 years with little maintenance aside from cleaning, odds favor Olympus. Steve A. McClain, MD *PS truth be told I also left the UV lamp on for a week 240 hours and burned up the UV lamp housing and had to replace that. Adding of course a $12 darkroom timer to prevent that accident from repeating. On May 8, 2017, at 13:09, "histonet-request at lists.utsouthwestern.edu" > wrote: Subject: [Histonet] Microscope selection Message-ID: > Content-Type: text/plain; charset=UTF-8 Hello to all Would like to have some advice from expert from the field of pathology on microscope selection Which one would you select as a manufacturer for microscopes 1- Olympus 2- Nikon 3- Leica From rjbuesa at yahoo.com Tue May 9 07:30:54 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 9 May 2017 12:30:54 +0000 (UTC) Subject: [Histonet] KOH softener to "decalcify" bone? In-Reply-To: References: Message-ID: <413119102.5495621.1494333054424@mail.yahoo.com> EDTA can be used to decalcify delicate bones, such as bona marrow core biopsies.KOH is used to soften keratin (such as in toe nails) but is totally ineffective for decalcification.Ren? On Monday, May 8, 2017 2:32 PM, Dorothy Hu via Histonet wrote: I know someone used KOH with EDTA together to decalcify bone. It is worthwhile to try KOH only to soft bone and kept dynamic labeling. If anyone had tried successfully, please let us know. Thanks. > > Today's Topics: > >? ? 1. Fabric softener to "decalcify" bone? (Angela Lamberth) >? ? 2. Re: Fabric softener to "decalcify" bone? - Downy has? ? ? formic >? ? ? acid?? (Angela Lamberth) > > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff at uropartners.com Tue May 9 08:20:49 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 9 May 2017 13:20:49 +0000 Subject: [Histonet] Adhesive cover for microscope state. Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF113BC261@COLOEXCH01.uropartners.local> Hello All: Years ago I had a white adhesive plastic cover on my microscope stage that kept the stage smooth. I am looking for a similar product but having no luck with an internet search. Does anyone have any leads on this? Thanks, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From Richard.Cartun at hhchealth.org Wed May 10 10:49:38 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Wed, 10 May 2017 15:49:38 +0000 Subject: [Histonet] NGS Testing Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E9541CA3D@HHCEXCHMB03.hhcsystem.org> I know this question is somewhat out of our domain, but are any of you engaged in "Next Generation Sequencing" (NGS) testing on patient FFPE cancer specimens? If so, what's your experience with insurance pre-certification and reimbursement? Thank you as always. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From lorjones at ingalls.org Wed May 10 12:16:43 2017 From: lorjones at ingalls.org (Lori Jones) Date: Wed, 10 May 2017 17:16:43 +0000 Subject: [Histonet] Disposal of Bouin's Solution Message-ID: <18386B8047F86E479002C017116AE82917E3BDE0@EXCHMAIL-PA-1V.ingalls.ihsil.org> We use Bouin's Solution in our pathology department and are currently disposing of it by neutralizing it with Vytac for formalin. I can't find supporting documentation that this is the proper way to dispose of it. I'd like to know if and how other institutions are neutralizing Bouin's Solution. Thank you! Lori Jones, CT(ASCP) Pathology Supervisor Ingalls Memorial Hospital ________________________________ This message and any included attachments are from Ingalls Memorial Hospital and are intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail with a copy to mailsecurity at ingalls.org Thank you From lorjones at ingalls.org Wed May 10 13:01:44 2017 From: lorjones at ingalls.org (Lori Jones) Date: Wed, 10 May 2017 18:01:44 +0000 Subject: [Histonet] Slide disposal Message-ID: <18386B8047F86E479002C017116AE82917E3BDF2@EXCHMAIL-PA-1V.ingalls.ihsil.org> We dispose of our slides (labels left on) in large sharps containers and have them hauled away by the waste disposal company. Lori Jones, CT(ASCP) Pathology Supervisor Ingalls Memorial Hospital ________________________________ This message and any included attachments are from Ingalls Memorial Hospital and are intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail with a copy to mailsecurity at ingalls.org Thank you From SteveM at mcclainlab.com Wed May 10 13:36:16 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Wed, 10 May 2017 18:36:16 +0000 Subject: [Histonet] PCR for tick bite vasculitis In-Reply-To: References: Message-ID: <7E5E2B0D-200D-44F1-AAC7-C59653969CAB@mcclainlab.com> Does anyone have experience or suggestions for where to send FFPE biopsy sections from a patient with erythema migrans and known Amblyomma tickbite. Dieterle negative, anti-Borrelia burgdorferii negative Gram negative PAS negative. Thanks for your help. Steve A McClain, MD 631-361-4000 From Timothy.Morken at ucsf.edu Thu May 11 10:17:58 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 11 May 2017 15:17:58 +0000 Subject: [Histonet] FW: PA Supervisor Position, San Francisco Message-ID: <761E2B5697F795489C8710BCC72141FF8E5DF8B1@ex07.net.ucsf.edu> We have a PA Supervisor position open at UCSF Medical Center. Oversees 3 grossing sites (3 hosptitals) and morgue/autopsy, trains residents. Oversees 9 PA's, 4 accessioners, administrative staff. Apply online at: http://jobs.ucsfmedicalcenter.org/ Job Description Pathologists' Assistants Supervisor - Pathology-Morgue (SPEC) Job ID: 12043 Job Code: 0461 Under minimal direction of the Directors, the incumbent serves as a supervisor and lead technical expert in the Surgical Pathology Gross Labs at Mission Bay, Parnassus and Mt Zion campuses, and in Autopsy at Parnassus, supervising and providing leadership and direction to certified and non-cerified Pathologist Assistants (PAs) and technical staff. The incumbent's primary goals for this position are to train and educate residents and staff on grossing large and complex cancer resection specimens and create an efficient Gross Lab and Autopsy service. In addition to serving as a practitioner, educator/role model for fellows, residents, and staff members on grossing complex specimens, it is the role of the supervisor to continuously interact and consult with the laboratory director(s), administrative director, lab managers, and designee. The incumbent determines the need to consult with pathologist, makes operational decisions, troubleshoots and resolves specimen receiving issues. The incumbent serves as a lead technical expert in grossing the most complex cancer specimens (pancreas, breast, prostate, GYN, etc.) as well as simple benign resections and biopsies. The incumbent must have an extensive knowledge of the full scope of general PA technical duties in the laboratory when required (lab preparation, receiving, accessioning, cryostat frozen sectioning, staining of stat operating room specimens, gross sectioning, dictation, photography, etc.). The incumbent will participate in resident education as pertains to gross lab activities and maintain the departmental and web-based surgical pathology manual. The incumbent must also have a thorough understanding of what sections are essential, and perform duties efficiently (focusing on quality and quantity). The incumbent's responsibilities include handling the competency program and providing education sessions to staff to learn to gross large resections and complex cancer specimens. As supervisor the incumbent recruits, hires, trains, completes performance evaluations, and resolves employee issues; provides orientation, completes competency assessments, maintains staff schedules, training and compliance documentation and operating procedure; maintains equipment for the laboratory and adheres to compliance requirements; proactively advises and assists faculty and residents planning research projects. As directed, the incumbent provides operational support in other managers' absence; as determined, works a variable schedule and rotates at other work sites as needed; provides technical and leadership support to the Electron Microscopy & Neuromuscular (EM & NM) Laboratory and Histology Laboratory as needed. Other duties are as assigned. The flexibility to orient and work at all UCSF Medical Center locations is required. Required Qualifications: * Previous leadership experience in a hospital laboratory * American Society for Clinical Pathology (ASCP) certification must be maintained as long as one works in this position * ASCP certified as Pathologists' Assistant * Bachelor's degree * Demonstrated proficiency in teaching, communication and interpersonal skills * Knowledge of medical terminology, specifically understanding of Pathology * Ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines * Excellent data entry, word processing, spelling, grammar, and editing skills * Ability to assume leadership responsibilities and assist with operational oversight of Gross Rooms The flexibility to orient and work at all UCSF Medical Center locations is required Preferred qualifications * Minimum five years' experience as an ASCP-certified PA * A graduate from a National Accrediting Agency for Clinical Laboratory Sciences (NAACLS) accredited pathologists' assistant training program * Master's degree * Current Fellow member of the American Association of Pathologists' Assistants (AAPA) (highly preferred) * Current Member of the American Society for Clinical Pathology * Previous leadership experience in a hospital laboratory Experience working in the Pathology Department of an academic health center Licensure: * American Society for Clinical Pathology (ASCP) certification must be maintained as long as one works in this position * ASCP certified as Pathologists' Assistant Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken at ucsfmedctr.org From jvickroy at SpringfieldClinic.com Thu May 11 10:59:51 2017 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Thu, 11 May 2017 15:59:51 +0000 Subject: [Histonet] Histology Manager - Technical Supervisor Message-ID: <9B1A1501A800064397369BD8072E6BCA6D4AD181@E2K10DB.springfieldclinic.com> We are reviewing the requirements in a job description for Histology Manager of a multi-specialty clinic. I am retiring next year and we are wanting to make sure that we have a good succession plan. With the dwindling number of certified HT's and HTL's and lack of CAP requirements to perform histology functions we are wondering how to list requirements for this position. I still believe a manager/supervisor should have a certification (HT or even HTL ) along with some years of histotech experience. Can someone enlighten me if there are specific CAP requirements for a Histology supervisor and how are others handling these positions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From rsrichmond at gmail.com Thu May 11 17:39:22 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 11 May 2017 18:39:22 -0400 Subject: [Histonet] Disposal of Bouin's Solution Message-ID: Lori Jones, CT(ASCP), Pathology Supervisor, Ingalls Memorial Hospital asks: >>We use Bouin's Solution in our pathology department and are currently disposing of it by neutralizing it with Vytac for formalin. I can't find supporting documentation that this is the proper way to dispose of it. I'd like to know if and how other institutions are neutralizing Bouin's solution.<< The best way to dispose of Bouin's fixative is by not buying it at all. What do you use it for? There are substitutes for it for most purposes. Bob Richmond Samurai Pathologist Maryville TN From TNMayer at mdanderson.org Fri May 12 10:22:35 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Fri, 12 May 2017 15:22:35 +0000 Subject: [Histonet] Histology Manager - Technical Supervisor (Vickroy, James) Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8839EE4F03@D1PWPEXMBX08.mdanderson.edu> Jim, HT/HTL certification, education level (just because someone has an HT doesn't mean they have a degree), minimum experience, supervisory or mgt experience, and intermediate computer knowledge. Graduation of an approved program is not necessary, if they hold HT/HTL cert. CLIA eligible to gross is good as well. Toysha ------------------------------ Message: 5 Date: Thu, 11 May 2017 15:59:51 +0000 From: "Vickroy, James" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Histology Manager - Technical Supervisor Message-ID: <9B1A1501A800064397369BD8072E6BCA6D4AD181 at E2K10DB.springfieldclinic.com> Content-Type: text/plain; charset="us-ascii" We are reviewing the requirements in a job description for Histology Manager of a multi-specialty clinic. I am retiring next year and we are wanting to make sure that we have a good succession plan. With the dwindling number of certified HT's and HTL's and lack of CAP requirements to perform histology functions we are wondering how to list requirements for this position. I still believe a manager/supervisor should have a certification (HT or even HTL ) along with some years of histotech experience. Can someone enlighten me if there are specific CAP requirements for a Histology supervisor and how are others handling these positions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 162, Issue 9 **************************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From FMonson at wcupa.edu Fri May 12 13:15:52 2017 From: FMonson at wcupa.edu (Monson, Frederick) Date: Fri, 12 May 2017 18:15:52 +0000 Subject: [Histonet] Disposal of Bouin's Solution In-Reply-To: References: Message-ID: <35d94a981043459b899e74a8ae9fef70@WCUXCHP08.PASSHE.LCL> https://oag.ca.gov/sites/all/files/agweb/pdfs/cci/safety/picric.pdf Two hazards: formaldehyde and picric acid (see attached). While I agree with Dr. Richmond, changing protocols is not easily done. So. You may search for appropriate oxidizers of aldehydes. OR!!! You may take the reasonable path by sending it off periodically to a company that will cost enough to raise the use of the stuff to serious conversation. Picric acid - since I used it from the late 1950's - the end of our "Dark Ages"[???] - into the mid 1970's -the age when students became consumers and knowing what was on the test was paramount - IS really hazardous - when it is dry. [So, I always kept it covered with water (saturated in local conditions.] Picric acid is the reason that you should either stop using Bouin's or waste it (wet!!) to a competent waste-handling company. Cheers, Fred Monson (5 weeks to the oblivion called 'retirement.' -----Original Message----- From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, May 11, 2017 6:39 PM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Disposal of Bouin's Solution Lori Jones, CT(ASCP), Pathology Supervisor, Ingalls Memorial Hospital asks: >>We use Bouin's Solution in our pathology department and are currently disposing of it by neutralizing it with Vytac for formalin. I can't find supporting documentation that this is the proper way to dispose of it. I'd like to know if and how other institutions are neutralizing Bouin's solution.<< The best way to dispose of Bouin's fixative is by not buying it at all. What do you use it for? There are substitutes for it for most purposes. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Fri May 12 13:35:28 2017 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 12 May 2017 18:35:28 +0000 Subject: [Histonet] Disposal of Bouin's Solution Message-ID: Not a disposal query. "There are substitutes for it for most purposes." Bob, I am interested in "most" and "substitutes" In what situation does "most" not apply? What substitutes? For example, I read that tissues are fixed in Picric acid-containing Formalin for IHC/IF. Why? What's the logic/rationale? Always listening/reading/asking/learning Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From POWELL_SA at mercer.edu Fri May 12 14:00:15 2017 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Fri, 12 May 2017 19:00:15 +0000 Subject: [Histonet] Disposal of Bouin's Solution In-Reply-To: <35d94a981043459b899e74a8ae9fef70@WCUXCHP08.PASSHE.LCL> References: <35d94a981043459b899e74a8ae9fef70@WCUXCHP08.PASSHE.LCL> Message-ID: <76672380b54746f18e56cc655f6b6b32@spiderman.MercerU.local> I agree it is very dangerous. When I discovered a dried bottle of picric acid someone had donated to the school right after I first came to the university. I had to call the fire department and the bomb squad came to get it. They took it to the firing range and blew a huge sized whole in the ground, this was a small bottle of picric acid. Of course we made the local news, but get rid of it if possible or keep it under water as Fred says. Shirley -----Original Message----- From: Monson, Frederick via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, May 12, 2017 2:16 PM To: Histonet at lists.utsouthwestern.edu Cc: Bob Richmond Subject: Re: [Histonet] Disposal of Bouin's Solution https://oag.ca.gov/sites/all/files/agweb/pdfs/cci/safety/picric.pdf Two hazards: formaldehyde and picric acid (see attached). While I agree with Dr. Richmond, changing protocols is not easily done. So. You may search for appropriate oxidizers of aldehydes. OR!!! You may take the reasonable path by sending it off periodically to a company that will cost enough to raise the use of the stuff to serious conversation. Picric acid - since I used it from the late 1950's - the end of our "Dark Ages"[???] - into the mid 1970's -the age when students became consumers and knowing what was on the test was paramount - IS really hazardous - when it is dry. [So, I always kept it covered with water (saturated in local conditions.] Picric acid is the reason that you should either stop using Bouin's or waste it (wet!!) to a competent waste-handling company. Cheers, Fred Monson (5 weeks to the oblivion called 'retirement.' -----Original Message----- From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, May 11, 2017 6:39 PM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Disposal of Bouin's Solution Lori Jones, CT(ASCP), Pathology Supervisor, Ingalls Memorial Hospital asks: >>We use Bouin's Solution in our pathology department and are currently disposing of it by neutralizing it with Vytac for formalin. I can't find supporting documentation that this is the proper way to dispose of it. I'd like to know if and how other institutions are neutralizing Bouin's solution.<< The best way to dispose of Bouin's fixative is by not buying it at all. What do you use it for? There are substitutes for it for most purposes. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Cindy at alliedsearchpartners.com Fri May 12 14:21:26 2017 From: Cindy at alliedsearchpartners.com (Cindy Moore) Date: Fri, 12 May 2017 19:21:26 +0000 Subject: [Histonet] Several Great Boston Area Career Opportunities Message-ID: Good afternoon. I'm a recruiter with Allied Search Partners, and we are working with several clients in the Boston, MA area on some great career opportunities. If you'd like to learn more about any of the following opportunities, please send your resume to me at cindy at alliedsearchpartners . com, so I can call you soon. 1. Histotechnologist, evening shift (Mon-Friday, 7pm to 3am), for hospital. 2. Histotech for research company 3. Histology Manager for research company 4. Pathology Office Manager, supervisory and Bachelor's required. Front Office transcription and medical terminology knowledge a must. 5. 4 Pathology Admin positions, 2 Assistant and 2 Executive Assistants. If Boston isn't your desired geography, BUT YOU ARE OPEN TO MAKING A CAREER CHANGE, please send your resume to me at cindy at alliedsearchpartners . com, and I will get back in touch with you to learn about your career and criteria for new opportunities. Thanks! Cindy Moore Recruiter, Laboratory Staffing Allied Search Partners cindy at alliedsearchpartners.com Phone: (888) 388-7571 ext. 105 From MMASSEY at HHCS.org Sat May 13 11:53:33 2017 From: MMASSEY at HHCS.org (Massey, Michelle) Date: Sat, 13 May 2017 16:53:33 +0000 Subject: [Histonet] HT job in Dalton,GA Message-ID: <991E8EC9-FF07-4346-B428-4110FBA9B586@HHCS.org> Just wanted to let all Histoneters know there is a job posting for a certified HT or HT certification eligible person at Hamilton Medical Center in Dalton GA. Day shift, no holidays, no weekends, no call. See Hamiltonhealth.com under Careers. From carl.hobbs at kcl.ac.uk Sat May 13 12:33:30 2017 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sat, 13 May 2017 17:33:30 +0000 Subject: [Histonet] Disposal of Bouin's Solution Message-ID: "I agree it is very dangerous.? When I discovered a dried bottle of picric acid someone had donated to the school right after I first came to the university.? I had to call the fire department and the bomb squad came to get it.? They took it to the firing range and blew a huge sized whole in the ground, this was a small bottle of picric acid.? Of course we made the local news, but get rid of it if possible or keep it under water as Fred says. " Chuckle....why didn't you just wet it? It would only be "dangerous " if you hit it with a hammer The explosive used was FAR bigger than the bang from one bottle of Picric acid Overkill. The story about a factory involved in Picric acid explosion involves ....several magnitudes higher than any volume stored in a Lab In the volumes used in a laboratory.....not really a problem unless used TOTALLY ?incompetently/maliciously Sure....if incompetence is there....you will get a bang akin to that one used to be able to buy from a Joke shop. FORMALIN is toxic, poisonous...it KILLS Don't use it! Do we stop using it? Hmmm In a well- run laboratory, I am FAR more concerned regarding the toxicity of stepping outside of my lab into the street where them cars are continuously spewing out far more toxic fumes. ALL OF THE TIME Yep....also the particulate matter from them car tyres that we breath in Does anyone regulate this? Nope! So, all you car drivers that are concerned re them small amounts of laboratory Picric acid....think again. Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From tgenade at gmail.com Sat May 13 12:34:40 2017 From: tgenade at gmail.com (Tyrone Genade) Date: Sat, 13 May 2017 12:34:40 -0500 Subject: [Histonet] Disposal of Bouin's Solution Message-ID: Hello, Regarding disposal of Bouin's solution the following protocol might be helpful/amusing/terrifying: https://phc.amedd.army.mil/PHC%20Resource%20Library/Bouins_fixative_solution_FS%20_37-007-0913.pdf For IHC the argument is that Bouin's doesn't destroy antigens as compared to other fixatives but I think this is a problem specific to certain antigens rather than a general issue---and those antigens can be preserved by other means now. It also seems to preserve nuclei much better. Bye -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From histo at pathlab.us Mon May 15 08:50:36 2017 From: histo at pathlab.us (Histology) Date: Mon, 15 May 2017 13:50:36 +0000 Subject: [Histonet] HT job in VA Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD820110B798@vdc01.domain.local> We have an HT position open in Norfolk, VA. M-F 6am-2Pm. Must be certified. No recruiters please. Email resume to histo at pathlab.us or fax to 757-664-9122 From ricki.simoskevitz at medtronic.com Mon May 15 11:23:35 2017 From: ricki.simoskevitz at medtronic.com (Simoskevitz, Ricki) Date: Mon, 15 May 2017 16:23:35 +0000 Subject: [Histonet] H&E Staining Procedure for bone samples embedded in Glycol Methacrylate Message-ID: Does anyone have a good H&E stain for bone sections in glycol methacrylate. There is alginate and collagen in the samples and it is making it very difficult to read and differentiate between the old host bone and the new bone or to see the osteoblasts and marrow. My information is listed below. Any and all help would be greatly appreciated. Thanks! Ricki Simoskevitz Scientist Group Leader OI Lab | Eatontown Quality Medtronic Osteoinductivity Lab 51 James Way| Eatontown, NJ/07724 | USA Office 732-542-2800 X6328|Fax 763-355-1224 ricki.simoskevitz at medtronic.com Medtronic.com|Facebook|Linkedin|Twitter|YouTube LET'S TAKE HEALTHCARE FURTHER, TOGETHER [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From areichard at lmhealth.org Mon May 15 11:39:23 2017 From: areichard at lmhealth.org (Amanda Reichard) Date: Mon, 15 May 2017 16:39:23 +0000 Subject: [Histonet] Stand Alone Heat Extractor Message-ID: <87d1e58d024947d096e96a2f08a96593@LMH-EXMB-1.lmhealth.org> Hi Everyone I am hoping someone can help me out here... I am in search of stand-alone heat extractors for our cryostat. I am told they are hard to come by and was curious if anyone knew of any companies that have them? The one I know of isn't an approved vendor yet, and I am trying to avoid going through that process. Thanks in advance! Amanda Reichard, HTL (ASCP)cm Histology/Cytology Supervisor Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (220) 564-4163 areichard at lmhealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 220-564-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From bakevictoria at gmail.com Mon May 15 11:58:56 2017 From: bakevictoria at gmail.com (Victoria Baker) Date: Mon, 15 May 2017 12:58:56 -0400 Subject: [Histonet] Stand Alone Heat Extractor In-Reply-To: <87d1e58d024947d096e96a2f08a96593@LMH-EXMB-1.lmhealth.org> References: <87d1e58d024947d096e96a2f08a96593@LMH-EXMB-1.lmhealth.org> Message-ID: ThermoFisher. It is on there website. I don't have the catalog number but I just ordered one On May 15, 2017 12:48 PM, "Amanda Reichard via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > Hi Everyone > > I am hoping someone can help me out here... I am in search of stand-alone > heat extractors for our cryostat. I am told they are hard to come by and > was curious if anyone knew of any companies that have them? The one I know > of isn't an approved vendor yet, and I am trying to avoid going through > that process. > > Thanks in advance! > > Amanda Reichard, HTL (ASCP)cm > Histology/Cytology Supervisor > Licking Memorial Health Systems > 1320 W. Main St. > Newark, OH 43055 > (220) 564-4163 > areichard at lmhealth.org > > > ________________________________ > > This e-mail, including attachments, is intended for the sole use of the > individual and/or entity to whom it is addressed, and contains information > from Licking Memorial Health Systems which is confidential or privileged. > If you are not the intended recipient, nor authorized to receive for the > intended recipient, be aware that any disclosure, copying, distribution or > use of the contents of this e-mail and attachments is prohibited. If you > have received this in error, please advise the sender by reply e-mail and > delete the message immediately. You may also contact the LMH Process > Improvement Center at 220-564-4641. E-mail transmissions cannot be > guaranteed to be secure or error-free as information could be intercepted, > corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. > The sender therefore does not accept liability for any errors or omissions > in the contents of this message, which arise as a result of e-mail > transmission. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cls71877 at gmail.com Mon May 15 12:54:40 2017 From: cls71877 at gmail.com (Cristi Rigazio) Date: Mon, 15 May 2017 13:54:40 -0400 Subject: [Histonet] Send-Outs Message-ID: Hi Histoland! We have been reviewing our send out procedures and I have been tasked with researching what TAT's other institutions define. Currently, we allow 48 hours for the preparation of "orders" to the time sent out. Is this reasonable? Do other facilities allow more or less time? I feel like Amazon can get something to my door step in a matter of hours, so I must be missing something obvious in our steps that could be simplified, but I can't put my finger on it. Any feedback on what others are doing is greatly appreciated. Sincerely, Cristi From vicki.kalscheur at wisc.edu Mon May 15 14:05:51 2017 From: vicki.kalscheur at wisc.edu (Vicki Kalscheur) Date: Mon, 15 May 2017 19:05:51 +0000 Subject: [Histonet] Ricki - glycol stains Message-ID: Perhaps browse the histonet archives for some tips on this staining. I regularly use PMMA stains ( that do not work on glycol). Good Luck. Vicki L. Kalscheur School of Veterinary Medicine Comparative Orthopedic Research Laboratory 2015 Linden Drive Madison, WI 53706-1100 Phone: 608-262-8534 vicki.kalscheur at wisc.edu From tkngflght at yahoo.com Mon May 15 14:56:30 2017 From: tkngflght at yahoo.com (Cheryl) Date: Mon, 15 May 2017 19:56:30 +0000 (UTC) Subject: [Histonet] cassette printers - multi color cassettes and network ready- References: <1070649568.1965650.1494878190288.ref@mail.yahoo.com> Message-ID: <1070649568.1965650.1494878190288@mail.yahoo.com> What are you using for your cassette printer? ?The Leica IP-C is a familiar model but it is SO BiG. We need 3-5 colors of cassettes, and it has to network so we can have multiple users. ?We print two lines (number and name) with hopes to go to full bar coding in the near future.? Looking for any/all input - vendors welcome, too.?Cheryl Kerry, HT(ASCP) ADG Pathologyckerry at adgpath.com From lblazek at digestivespecialists.com Tue May 16 06:21:08 2017 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Tue, 16 May 2017 07:21:08 -0400 Subject: [Histonet] cassette printers - multi color cassettes and network ready- In-Reply-To: <1070649568.1965650.1494878190288@mail.yahoo.com> References: <1070649568.1965650.1494878190288.ref@mail.yahoo.com> <1070649568.1965650.1494878190288@mail.yahoo.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E391A45758917@IBMB7Exchange.digestivespecialists.com> Cheryl, We use the Primera printer and have had great success with it. It's small and reliable. You can get it from http://cwsincorp.com/ Linda Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Cheryl via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 15, 2017 3:57 PM To: Histonet Subject: [Histonet] cassette printers - multi color cassettes and network ready- What are you using for your cassette printer? ?The Leica IP-C is a familiar model but it is SO BiG. We need 3-5 colors of cassettes, and it has to network so we can have multiple users. ?We print two lines (number and name) with hopes to go to full bar coding in the near future. Looking for any/all input - vendors welcome, too.?Cheryl Kerry, HT(ASCP) ADG Pathologyckerry at adgpath.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Tue May 16 10:34:13 2017 From: patpxs at gmail.com (P Sicurello) Date: Tue, 16 May 2017 08:34:13 -0700 Subject: [Histonet] A Question About Paraffin Times Message-ID: Good Morning Listers, I am asking the collective wisdom of the Histonet this question: Is it better to remove baskets from the processor on Saturday morning and: A. Let the cassettes freeze, then melt them down and embed Monday morning? OR B. Leave the cassettes in molten paraffin and embed Monday morning? I am of the opinion that leaving the samples (not fatty, like breast cores) in molten paraffin (62 degrees C) is bad practice, and causes them to get "crunchy", among other things. What do you think? Thank in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From sandra.cheasty at wisc.edu Tue May 16 10:53:34 2017 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Tue, 16 May 2017 15:53:34 +0000 Subject: [Histonet] Extra Large Cassette Clamps for Microtomes Message-ID: Hello all, I am looking for opinions on giant block clamps for the Leica RM2235 and Microm HM355S microtomes. * I know CellPath makes one, are their others? * Is it easy to swap them out for a regular clamp, or is it advisable to dedicate one microtome for these huge cassettes. Thank you! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From Timothy.Morken at ucsf.edu Tue May 16 10:53:16 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 16 May 2017 15:53:16 +0000 Subject: [Histonet] Send-Outs In-Reply-To: References: Message-ID: Really? You want to compare your lab to Amazon? If you have stock on-hand, located in one place, have robots to retrieve it, people who do nothing but pack boxes and trucks standing by to take any order at any time of day, then you are in the league of Amazon and can receive an order and send it out within an hour. However most of us first need to find the material to send out - slides? where are they? Pathologist, resident? waiting to be filed? Removed from file by someone else? Can't find anything in THAT office...Same with blocks - not in file, is there a card telling who took it? Maybe for recuts, maybe for research, then back into the batch to be filed. Look 5 different places. Frozen tissue is usually easier but, whoops...already sent some out for another requested test... Anyway, that is what my experience is! We don't have any set time requirement. It could be an hour or a few days depending on if can easily find the material they want to send. Usually it is within a day. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cristi Rigazio via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 15, 2017 10:55 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Send-Outs Hi Histoland! We have been reviewing our send out procedures and I have been tasked with researching what TAT's other institutions define. Currently, we allow 48 hours for the preparation of "orders" to the time sent out. Is this reasonable? Do other facilities allow more or less time? I feel like Amazon can get something to my door step in a matter of hours, so I must be missing something obvious in our steps that could be simplified, but I can't put my finger on it. Any feedback on what others are doing is greatly appreciated. Sincerely, Cristi _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Tue May 16 10:57:45 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 16 May 2017 15:57:45 +0000 (UTC) Subject: [Histonet] A Question About Paraffin Times In-Reply-To: References: Message-ID: <795806025.531990.1494950265387@mail.yahoo.com> I consider taking out ? freezing ? ?melting ? casting blocks is worse than leaving the tissues in molten paraffin.It seems that your Monday tissue processing ends on Saturday. I suggest to process your tissues with a "delay" (most tissue processers have this feature) and leave? them more time in formalin and make coincide their ending time with your Monday start time.Ren? On Tuesday, May 16, 2017 11:51 AM, P Sicurello via Histonet wrote: Good Morning Listers, I am asking the collective wisdom of the Histonet this question: Is it better to remove baskets from the processor on Saturday morning and: A.? Let the cassettes freeze, then melt them down and embed Monday morning? OR B.? Leave the cassettes in molten paraffin and embed Monday morning? I am of the opinion that leaving the samples (not fatty, like breast cores) in molten paraffin (62 degrees C)? is bad practice, and causes them to get "crunchy", among other things. What do you think? Thank in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet | | Virus-free. www.avast.com | From naje1972 at yahoo.com Tue May 16 10:59:26 2017 From: naje1972 at yahoo.com (cynthia haynes) Date: Tue, 16 May 2017 15:59:26 +0000 (UTC) Subject: [Histonet] A Question About Paraffin Times In-Reply-To: References: Message-ID: <1670685660.735422.1494950366751@mail.yahoo.com> Please don't leave the tissue in the hot wax.They will harden and be brittle. Cynthia James H.T? Sent from Yahoo Mail on Android On Tue, May 16, 2017 at 10:50 AM, P Sicurello via Histonet wrote: Good Morning Listers, I am asking the collective wisdom of the Histonet this question: Is it better to remove baskets from the processor on Saturday morning and: A.? Let the cassettes freeze, then melt them down and embed Monday morning? OR B.? Leave the cassettes in molten paraffin and embed Monday morning? I am of the opinion that leaving the samples (not fatty, like breast cores) in molten paraffin (62 degrees C)? is bad practice, and causes them to get "crunchy", among other things. What do you think? Thank in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun at hhchealth.org Tue May 16 11:25:37 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 16 May 2017 16:25:37 +0000 Subject: [Histonet] Send-Outs In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E95429CFD@HHCEXCHMB03.hhcsystem.org> Do you have a Pathology Residency Program? If so, good luck with a policy that states that requests will be sent out in 48 hours. Our policy is 3-5 business days once we receive the request. Having said that, most requests are processed in 2-3 days. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 16, 2017 11:53 AM To: Cristi Rigazio Cc: Histonet Subject: Re: [Histonet] Send-Outs Really? You want to compare your lab to Amazon? If you have stock on-hand, located in one place, have robots to retrieve it, people who do nothing but pack boxes and trucks standing by to take any order at any time of day, then you are in the league of Amazon and can receive an order and send it out within an hour. However most of us first need to find the material to send out - slides? where are they? Pathologist, resident? waiting to be filed? Removed from file by someone else? Can't find anything in THAT office...Same with blocks - not in file, is there a card telling who took it? Maybe for recuts, maybe for research, then back into the batch to be filed. Look 5 different places. Frozen tissue is usually easier but, whoops...already sent some out for another requested test... Anyway, that is what my experience is! We don't have any set time requirement. It could be an hour or a few days depending on if can easily find the material they want to send. Usually it is within a day. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cristi Rigazio via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 15, 2017 10:55 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Send-Outs Hi Histoland! We have been reviewing our send out procedures and I have been tasked with researching what TAT's other institutions define. Currently, we allow 48 hours for the preparation of "orders" to the time sent out. Is this reasonable? Do other facilities allow more or less time? I feel like Amazon can get something to my door step in a matter of hours, so I must be missing something obvious in our steps that could be simplified, but I can't put my finger on it. Any feedback on what others are doing is greatly appreciated. Sincerely, Cristi _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From tkngflght at yahoo.com Tue May 16 11:26:55 2017 From: tkngflght at yahoo.com (Cheryl) Date: Tue, 16 May 2017 16:26:55 +0000 (UTC) Subject: [Histonet] cassette printers - multi color cassettes and network ready- In-Reply-To: <8BE14B3077AE9A449557AC6019C8D31B5BC42B7D@SERVER.cw.local> References: <1070649568.1965650.1494878190288.ref@mail.yahoo.com> <1070649568.1965650.1494878190288@mail.yahoo.com> <8BE14B3077AE9A449557AC6019C8D31B5BC42B7D@SERVER.cw.local> Message-ID: <355659027.673159.1494952015638@mail.yahoo.com> Thanks to those sending me printer information. ?There were a couple we didn't know existed !?Cheryl ??? ? -----Original Message----- From: Cheryl via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 15, 2017 12:57 PM To: Histonet Subject: [Histonet] cassette printers - multi color cassettes and network ready- What are you using for your cassette printer? ?? We need 3-5 colors of cassettes, and it has to network so we can have multiple users. ?We print two lines (number and name) with hopes to go to full bar coding in the near future. Looking for any/all input - vendors welcome, too.?Cheryl Kerry, HT(ASCP) ADG Pathology ?ckerry at adgpath.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Tue May 16 13:06:56 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 16 May 2017 18:06:56 +0000 Subject: [Histonet] Send-outs, cassette printers, holding cassettes, and cassette clamps Message-ID: <48E053DDF6CE074DB6A7414BA05403F8135567@HRHEX03-HOS.holyredeemer.local> 1. Send-Outs (Cristi Rigazio) Our TAT for send-outs is 1 working day (M-F). The office staff here use the "Slide/block Send Out" function in CoPath to track all material moving in and out of Anatomic Pathology and it really speed up retrieval of case material 3. Cassette printers - multi color cassettes and network ready- (Cheryl) Even though they are big, we still love our Leica Printers. They are such workhorses and they were so easy to interface. 5. A Question About Paraffin Times (P Sicurello) I agree that if possible, the delay should be in formalin, but if you are running into a problem where that might exceed fixation times for a breast specimen there are 2 good alternatives. The first one is as you stated, to just pull them out of paraffin and let them chill, OR you can program a "hold" in 70% alcohol to avoid formalin fixation beyond 72 hours, and have the process finish at the usual time. Works like a charm. Feel free to contact me for details. I would never leave cassettes to "cook" in hot paraffin. 6. Extra Large Cassette Clamps for Microtomes (Sandra Cheasty) Only use one microtome, even if you are swapping out. Just my personal, unscientific opinion, but the less one disturbs the clamp/knife holder assembly, the better. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor, Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From Shannon.Logan at bellin.org Tue May 16 13:13:37 2017 From: Shannon.Logan at bellin.org (Logan, Shannon) Date: Tue, 16 May 2017 18:13:37 +0000 Subject: [Histonet] A Question About Paraffin Times In-Reply-To: References: Message-ID: Hello Paula, So why must the cassettes be removed on Saturday morning if you aren't embedding until Monday morning? Don't you have a "delay start" feature on your processor? Neither option A or B seem like a good thing for the tissue! We time our processor to finish at 5 AM Monday when the first Histotech arrives for embedding. The cassettes remain in formalin until the processor starts up on Sunday evening. Regards, Shannon H. Logan B.S., HTL (ASCP) Pathology Department Bellin Health Memorial Hospital 744 South Webster Avenue Green Bay, WI 54305-3400 920-433-3653 X3727 From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 16, 2017 10:34 AM To: HistoNet Subject: [Histonet] A Question About Paraffin Times Good Morning Listers, I am asking the collective wisdom of the Histonet this question: Is it better to remove baskets from the processor on Saturday morning and: A. Let the cassettes freeze, then melt them down and embed Monday morning? OR B. Leave the cassettes in molten paraffin and embed Monday morning? I am of the opinion that leaving the samples (not fatty, like breast cores) in molten paraffin (62 degrees C) is bad practice, and causes them to get "crunchy", among other things. What do you think? Thank in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Tue May 16 13:30:25 2017 From: patpxs at gmail.com (P Sicurello) Date: Tue, 16 May 2017 11:30:25 -0700 Subject: [Histonet] A Question About Paraffin Times In-Reply-To: References: Message-ID: Hi Shannon and Everyone Else, Usually the samples are placed on a weekend delay. This time, however, the breast cases were sitting in formalin since Thursday and the run had to end on Saturday due to the CAP 72 hour rule for breast and HER2 testing. The problem is, the person in charge of Histology not a Histologist. The person in charge was told by several experienced histotechs that letting the samples sit in molten paraffin is not a good thing to do. They were told that it was routine (which it isn't) to let them sit in hot wax for days. I just need information that states that sitting in paraffin for any excessive length of time is bad. I found it mentioned in Sheehan and Hrapcek's "Theory and Practice of Histotechnology". I can't find my Carson. Does she mention excessive times in paraffin? If so, please let me know. Thank you for all the assistance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Tue, May 16, 2017 at 11:13 AM, Logan, Shannon wrote: > Hello Paula, > > So why must the cassettes be removed on Saturday morning if you aren?t > embedding until Monday morning? > > Don?t you have a ?delay start? feature on your processor? Neither option A > or B seem like a good thing for the tissue! > > We time our processor to finish at 5 AM Monday when the first Histotech > arrives for embedding. > > The cassettes remain in formalin until the processor starts up on Sunday > evening. > > > > Regards, > > > > > > Shannon H. Logan B.S., HTL (ASCP) > > Pathology Department > > > > Bellin Health Memorial Hospital > > 744 South Webster Avenue > > Green Bay, WI 54305-3400 > > 920-433-3653 X3727 <(920)%20433-3653> > > > > > > > > *From:* P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] > > *Sent:* Tuesday, May 16, 2017 10:34 AM > *To:* HistoNet > *Subject:* [Histonet] A Question About Paraffin Times > > > > Good Morning Listers, > > I am asking the collective wisdom of the Histonet this question: > > > Is it better to remove baskets from the processor on Saturday morning and: > > A. Let the cassettes freeze, then melt them down and embed Monday morning? > OR > B. Leave the cassettes in molten paraffin and embed Monday morning? > > > I am of the opinion that leaving the samples (not fatty, like breast cores) > in molten paraffin (62 degrees C) is bad practice, and causes them to get > "crunchy", among other things. > > What do you think? > > Thank in advance. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 <(619)%20543-2872> <#> > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Michelle.Dragoo at nm.org Tue May 16 13:37:13 2017 From: Michelle.Dragoo at nm.org (Dragoo, Michelle) Date: Tue, 16 May 2017 18:37:13 +0000 Subject: [Histonet] Send-Outs In-Reply-To: References: Message-ID: We are the same as Tim. Typically we try to get things out within 24 hours, that said, it depends on how old the case is, does it need recuts etc. etc. I think your current time of 48 hours sounds quite reasonable. Michelle R. Dragoo, MBA, HT (ASCP) Supervisor, Histology Northwestern Medicine Central Dupage Hospital 25 North Winfield Road, Winfield IL 60190 O: 630.933.6748 michelle.dragoo at nm.org -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Tuesday, May 16, 2017 10:53 AM To: Cristi Rigazio Cc: Histonet Subject: Re: [Histonet] Send-Outs Really? You want to compare your lab to Amazon? If you have stock on-hand, located in one place, have robots to retrieve it, people who do nothing but pack boxes and trucks standing by to take any order at any time of day, then you are in the league of Amazon and can receive an order and send it out within an hour. However most of us first need to find the material to send out - slides? where are they? Pathologist, resident? waiting to be filed? Removed from file by someone else? Can't find anything in THAT office...Same with blocks - not in file, is there a card telling who took it? Maybe for recuts, maybe for research, then back into the batch to be filed. Look 5 different places. Frozen tissue is usually easier but, whoops...already sent some out for another requested test... Anyway, that is what my experience is! We don't have any set time requirement. It could be an hour or a few days depending on if can easily find the material they want to send. Usually it is within a day. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cristi Rigazio via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 15, 2017 10:55 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Send-Outs Hi Histoland! We have been reviewing our send out procedures and I have been tasked with researching what TAT's other institutions define. Currently, we allow 48 hours for the preparation of "orders" to the time sent out. Is this reasonable? Do other facilities allow more or less time? I feel like Amazon can get something to my door step in a matter of hours, so I must be missing something obvious in our steps that could be simplified, but I can't put my finger on it. Any feedback on what others are doing is greatly appreciated. Sincerely, Cristi _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From lori.garcia at medtronic.com Tue May 16 13:47:47 2017 From: lori.garcia at medtronic.com (Garcia, Lori, M.Sc.) Date: Tue, 16 May 2017 18:47:47 +0000 Subject: [Histonet] A Question About Paraffin Times In-Reply-To: References: Message-ID: <678a0010bfbe4ab690aa4b7519dd7d71@mspm1bmsgm11a.ent.core.medtronic.com> Hi Paula, I am in agreement with everyone else about putting the processor on a delay so it will finish when needed. However, if that is not possible, it is perfectly fine for the blocks to harden at room temp, not freezing, and then be warmed up again for embedding. This has come up in my research lab, and we haven't had any problems. Regards, Lori This message has been marked as Medtronic Controlled -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 16, 2017 11:30 AM To: Logan, Shannon Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] A Question About Paraffin Times Hi Shannon and Everyone Else, Usually the samples are placed on a weekend delay. This time, however, the breast cases were sitting in formalin since Thursday and the run had to end on Saturday due to the CAP 72 hour rule for breast and HER2 testing. The problem is, the person in charge of Histology not a Histologist. The person in charge was told by several experienced histotechs that letting the samples sit in molten paraffin is not a good thing to do. They were told that it was routine (which it isn't) to let them sit in hot wax for days. I just need information that states that sitting in paraffin for any excessive length of time is bad. I found it mentioned in Sheehan and Hrapcek's "Theory and Practice of Histotechnology". I can't find my Carson. Does she mention excessive times in paraffin? If so, please let me know. Thank you for all the assistance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Tue, May 16, 2017 at 11:13 AM, Logan, Shannon wrote: > Hello Paula, > > So why must the cassettes be removed on Saturday morning if you aren?t > embedding until Monday morning? > > Don?t you have a ?delay start? feature on your processor? Neither > option A or B seem like a good thing for the tissue! > > We time our processor to finish at 5 AM Monday when the first > Histotech arrives for embedding. > > The cassettes remain in formalin until the processor starts up on > Sunday evening. > > > > Regards, > > > > > > Shannon H. Logan B.S., HTL (ASCP) > > Pathology Department > > > > Bellin Health Memorial Hospital > > 744 South Webster Avenue > > Green Bay, WI 54305-3400 > > 920-433-3653 X3727 <(920)%20433-3653> > > > > > > > > *From:* P Sicurello via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > > *Sent:* Tuesday, May 16, 2017 10:34 AM > *To:* HistoNet > *Subject:* [Histonet] A Question About Paraffin Times > > > > Good Morning Listers, > > I am asking the collective wisdom of the Histonet this question: > > > Is it better to remove baskets from the processor on Saturday morning and: > > A. Let the cassettes freeze, then melt them down and embed Monday morning? > OR > B. Leave the cassettes in molten paraffin and embed Monday morning? > > > I am of the opinion that leaving the samples (not fatty, like breast > cores) in molten paraffin (62 degrees C) is bad practice, and causes > them to get "crunchy", among other things. > > What do you think? > > Thank in advance. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 <(619)%20543-2872> <#> > > > > *Confidentiality Notice*: The information transmitted in this e-mail > is intended only for the person or entity to which it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action > in reliance upon this information by persons or entities other than > the intended recipient is prohibited. If you received this e-mail in > error, please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From Heather.Seeley at tenethealth.com Tue May 16 13:48:40 2017 From: Heather.Seeley at tenethealth.com (Seeley, Heather) Date: Tue, 16 May 2017 18:48:40 +0000 Subject: [Histonet] A Question About Paraffin Times In-Reply-To: References: Message-ID: <9DFE334E776E734D9D231EF60CCE93C5878F8B98@TENHDCTHMB10-31.tenethealth.net> We do a delay protocol as well. The tissue samples will sit in formalin and start processing Sunday night for a normal amount of time to come off Monday morning. If this is not a possibility, it would be better to let them harden in the paraffin than to leave them in the hot paraffin, as this will lead to the tissue becoming brittle and very difficult to section. HEATHER SEELEY, HT(ASCP) Histotech 803-985-4676 OFFICE 803-327-7598 FAX ________________________________________ From: P Sicurello [patpxs at gmail.com] Sent: Tuesday, May 16, 2017 11:34 AM To: HistoNet Subject: [Histonet] A Question About Paraffin Times Good Morning Listers, I am asking the collective wisdom of the Histonet this question: Is it better to remove baskets from the processor on Saturday morning and: A. Let the cassettes freeze, then melt them down and embed Monday morning? OR B. Leave the cassettes in molten paraffin and embed Monday morning? I am of the opinion that leaving the samples (not fatty, like breast cores) in molten paraffin (62 degrees C) is bad practice, and causes them to get "crunchy", among other things. What do you think? Thank in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From CBird at amli-denton.com Tue May 16 13:48:53 2017 From: CBird at amli-denton.com (Cindy Bird) Date: Tue, 16 May 2017 13:48:53 -0500 Subject: [Histonet] A Question About Paraffin Times In-Reply-To: References: Message-ID: I agree? Sent from my iPhone > On May 16, 2017, at 1:28 PM, Logan, Shannon via Histonet wrote: > > Hello Paula, > So why must the cassettes be removed on Saturday morning if you aren't embedding until Monday morning? > Don't you have a "delay start" feature on your processor? Neither option A or B seem like a good thing for the tissue! > We time our processor to finish at 5 AM Monday when the first Histotech arrives for embedding. > The cassettes remain in formalin until the processor starts up on Sunday evening. > > Regards, > > > Shannon H. Logan B.S., HTL (ASCP) > Pathology Department > > Bellin Health Memorial Hospital > 744 South Webster Avenue > Green Bay, WI 54305-3400 > 920-433-3653 X3727 > > > > From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, May 16, 2017 10:34 AM > To: HistoNet > Subject: [Histonet] A Question About Paraffin Times > > Good Morning Listers, > > I am asking the collective wisdom of the Histonet this question: > > > Is it better to remove baskets from the processor on Saturday morning and: > > A. Let the cassettes freeze, then melt them down and embed Monday morning? > OR > B. Leave the cassettes in molten paraffin and embed Monday morning? > > > I am of the opinion that leaving the samples (not fatty, like breast cores) > in molten paraffin (62 degrees C) is bad practice, and causes them to get > "crunchy", among other things. > > What do you think? > > Thank in advance. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 <#> > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joyce.weems at emoryhealthcare.org Tue May 16 14:14:52 2017 From: joyce.weems at emoryhealthcare.org (Weems, Joyce K.) Date: Tue, 16 May 2017 19:14:52 +0000 Subject: [Histonet] A Question About Paraffin Times In-Reply-To: References: Message-ID: Must better to remove them on Sat than to leave in the hot paraffin. We do this all the time so we can meet the CAP time in formalin for the breasts. Good luck! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax 770-380-8099 Cell joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 16, 2017 11:34 AM To: HistoNet Subject: [Histonet] A Question About Paraffin Times Good Morning Listers, I am asking the collective wisdom of the Histonet this question: Is it better to remove baskets from the processor on Saturday morning and: A. Let the cassettes freeze, then melt them down and embed Monday morning? OR B. Leave the cassettes in molten paraffin and embed Monday morning? I am of the opinion that leaving the samples (not fatty, like breast cores) in molten paraffin (62 degrees C) is bad practice, and causes them to get "crunchy", among other things. What do you think? Thank in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From joyce.weems at emoryhealthcare.org Tue May 16 14:21:26 2017 From: joyce.weems at emoryhealthcare.org (Weems, Joyce K.) Date: Tue, 16 May 2017 19:21:26 +0000 Subject: [Histonet] A Question About Paraffin Times In-Reply-To: References: Message-ID: And that should say MUCH better.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax 770-380-8099 Cell joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Weems, Joyce K. via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 16, 2017 3:15 PM To: P Sicurello Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] A Question About Paraffin Times Must better to remove them on Sat than to leave in the hot paraffin. We do this all the time so we can meet the CAP time in formalin for the breasts. Good luck! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax 770-380-8099 Cell joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 16, 2017 11:34 AM To: HistoNet Subject: [Histonet] A Question About Paraffin Times Good Morning Listers, I am asking the collective wisdom of the Histonet this question: Is it better to remove baskets from the processor on Saturday morning and: A. Let the cassettes freeze, then melt them down and embed Monday morning? OR B. Leave the cassettes in molten paraffin and embed Monday morning? I am of the opinion that leaving the samples (not fatty, like breast cores) in molten paraffin (62 degrees C) is bad practice, and causes them to get "crunchy", among other things. What do you think? Thank in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cls71877 at gmail.com Tue May 16 16:19:55 2017 From: cls71877 at gmail.com (Cristi Rigazio) Date: Tue, 16 May 2017 17:19:55 -0400 Subject: [Histonet] Send-Outs In-Reply-To: References: Message-ID: <621E7ABB-2AA9-4175-8B3E-24BE58088CAE@gmail.com> Thanks everyone for the responses! And just to make sure...I was joking with the Amazon reference, lol! But I do aim high! Sent from my iPhone > On May 16, 2017, at 2:37 PM, Dragoo, Michelle wrote: > > We are the same as Tim. Typically we try to get things out within 24 hours, that said, it depends on how old the case is, does it need recuts etc. etc. I think your current time of 48 hours sounds quite reasonable. > > Michelle R. Dragoo, MBA, HT (ASCP) > Supervisor, Histology > Northwestern Medicine Central Dupage Hospital > 25 North Winfield Road, Winfield IL 60190 > O: 630.933.6748 > michelle.dragoo at nm.org > > > > > > -----Original Message----- > From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] > Sent: Tuesday, May 16, 2017 10:53 AM > To: Cristi Rigazio > Cc: Histonet > Subject: Re: [Histonet] Send-Outs > > Really? You want to compare your lab to Amazon? If you have stock on-hand, located in one place, have robots to retrieve it, people who do nothing but pack boxes and trucks standing by to take any order at any time of day, then you are in the league of Amazon and can receive an order and send it out within an hour. > > However most of us first need to find the material to send out - slides? where are they? Pathologist, resident? waiting to be filed? Removed from file by someone else? Can't find anything in THAT office...Same with blocks - not in file, is there a card telling who took it? Maybe for recuts, maybe for research, then back into the batch to be filed. Look 5 different places. Frozen tissue is usually easier but, whoops...already sent some out for another requested test... > > Anyway, that is what my experience is! > > We don't have any set time requirement. It could be an hour or a few days depending on if can easily find the material they want to send. Usually it is within a day. > > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center > > > > -----Original Message----- > From: Cristi Rigazio via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Monday, May 15, 2017 10:55 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Send-Outs > > Hi Histoland! > > We have been reviewing our send out procedures and I have been tasked with researching what TAT's other institutions define. Currently, we allow 48 hours for the preparation of "orders" to the time sent out. Is this reasonable? Do other facilities allow more or less time? > > I feel like Amazon can get something to my door step in a matter of hours, so I must be missing something obvious in our steps that could be simplified, but I can't put my finger on it. Any feedback on what others are doing is greatly appreciated. > > Sincerely, > Cristi > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. > From Evelyn.Flynn at childrens.harvard.edu Tue May 16 16:38:53 2017 From: Evelyn.Flynn at childrens.harvard.edu (Flynn, Evelyn) Date: Tue, 16 May 2017 21:38:53 +0000 Subject: [Histonet] Paraffin times; paraffin embedding station Message-ID: <1494970733443.60128@childrens.harvard.edu> Hello, Not to be the devil's advocate, but in our research lab we routinely put bone specimens in a vacuum oven at 60 degrees overnight or several days to promote better infiltration after processing. Somewhere I was taught that specimens are not damaged in the paraffin bath as long as the temperature is at 60 degrees, not higher. I would like to thank those Histonetters who suggested a Sakura Tissue-Tek embedding station when we were purchasing such an item. We have been very satisfied with the unit, and it looks very nice in the lab. Evelyn Flynn Boston Children's Hospital From relia1 at earthlink.net Wed May 17 08:38:23 2017 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 17 May 2017 09:38:23 -0400 Subject: [Histonet] Exciting Opportunities for Entry Level Histotechs!! 5-2017 Message-ID: <00ba01d2cf12$e9da1430$bd8e3c90$@earthlink.net> Hello Histonetters, I hope you are having a great day. I wanted to contact you because I have some exciting news!! I am working with several employers in some of the most exciting areas of the country who are SPECIFICALLY looking for new and recent graduates of histology programs. My clients offer excellent pay, benefits, relocation assistance and the opportunity for entry level and junior techs to start their careers on the right foot. They will utilize the skills they have and learn new things. The areas the positions are in are: Chicago, IL San Francisco, CA San Diego, CA Cranford, NJ For more information I can be reached at relia1 at earthlink.net or toll free at 866-607-3542 or on my cell at 407-353-5070. Thanks and Have a Great Day!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From tmcampbe at fmh.org Wed May 17 09:50:52 2017 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Wed, 17 May 2017 14:50:52 +0000 Subject: [Histonet] Milestone RHS-1 Accessories Message-ID: Hi all, Does anyone have any of the histomodules for the milestone RHS-1 110 cassette rack they would like to sell? I need 3. I need the glass containers, lids, stirrers, carriers, the disks and the heating disk. Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 From brannon at alliedsearchpartners.com Wed May 17 14:40:39 2017 From: brannon at alliedsearchpartners.com (Brannon Owens) Date: Wed, 17 May 2017 19:40:39 +0000 Subject: [Histonet] Histotech and Histology Supervisor job openings in Birmingham, AL Message-ID: We are currently seeking candidates for several openings in the Birmingham, AL area. Relocation assistance is offered! Position 1: Histology Supervisor (3rd shift) Position 2: Histotechnician (1st shift) Position 3: Histotechnician (3rd shift) Full job descriptions available on the Allied Search Partners website as well as additional opportunities in the field of histology. We look forward to hearing from you! Thank you, To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers/ Brannon Owens VP/Director of Recruitment Allied Search Partners LinkedIn: https://www.linkedin.com/in/jbrannonowens/ http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 Direct Line: 407.413.9421 F: 888.388.7572 From tony.henwood at health.nsw.gov.au Wed May 17 18:26:24 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 17 May 2017 23:26:24 +0000 Subject: [Histonet] A Question About Paraffin Times In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF95554CC0@SVDCMBX-MEX008.nswhealth.net> It is best to remove the blocks and let them set at room temperature. Leaving them at 60 or more degrees can adversely affect antigens. We routinely do this with our autopsies and have not noted any issues. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 17 May 2017 1:34 AM To: HistoNet Subject: [Histonet] A Question About Paraffin Times Good Morning Listers, I am asking the collective wisdom of the Histonet this question: Is it better to remove baskets from the processor on Saturday morning and: A. Let the cassettes freeze, then melt them down and embed Monday morning? OR B. Leave the cassettes in molten paraffin and embed Monday morning? I am of the opinion that leaving the samples (not fatty, like breast cores) in molten paraffin (62 degrees C) is bad practice, and causes them to get "crunchy", among other things. What do you think? Thank in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From garreyf at gmail.com Thu May 18 06:23:50 2017 From: garreyf at gmail.com (Garrey Faller) Date: Thu, 18 May 2017 07:23:50 -0400 Subject: [Histonet] A Question About Paraffin Times In-Reply-To: References: Message-ID: I have had to deal with this in the past and could not find an answer . My intuition was that it can't be good to leave in hot wax. So, on the rare occasion when my only Histotech called in sick I had to take the blocks out and cool them down. When ready they were put back in the tissue embedder to heat up and everything was fine. That's my experience, Garrey Sent from my iPhone > On May 16, 2017, at 2:48 PM, Cindy Bird via Histonet wrote: > > I agree? > > Sent from my iPhone > >> On May 16, 2017, at 1:28 PM, Logan, Shannon via Histonet wrote: >> >> Hello Paula, >> So why must the cassettes be removed on Saturday morning if you aren't embedding until Monday morning? >> Don't you have a "delay start" feature on your processor? Neither option A or B seem like a good thing for the tissue! >> We time our processor to finish at 5 AM Monday when the first Histotech arrives for embedding. >> The cassettes remain in formalin until the processor starts up on Sunday evening. >> >> Regards, >> >> >> Shannon H. Logan B.S., HTL (ASCP) >> Pathology Department >> >> Bellin Health Memorial Hospital >> 744 South Webster Avenue >> Green Bay, WI 54305-3400 >> 920-433-3653 X3727 >> >> >> >> From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] >> Sent: Tuesday, May 16, 2017 10:34 AM >> To: HistoNet >> Subject: [Histonet] A Question About Paraffin Times >> >> Good Morning Listers, >> >> I am asking the collective wisdom of the Histonet this question: >> >> >> Is it better to remove baskets from the processor on Saturday morning and: >> >> A. Let the cassettes freeze, then melt them down and embed Monday morning? >> OR >> B. Leave the cassettes in molten paraffin and embed Monday morning? >> >> >> I am of the opinion that leaving the samples (not fatty, like breast cores) >> in molten paraffin (62 degrees C) is bad practice, and causes them to get >> "crunchy", among other things. >> >> What do you think? >> >> Thank in advance. >> >> Sincerely, >> >> Paula Sicurello, HTL (ASCP)CM >> >> Histotechnology Specialist >> >> UC San Diego Health >> >> 200 Arbor Drive >> >> San Diego, CA 92103 >> >> (P): 619-543-2872 <#> >> >> >> >> *Confidentiality Notice*: The information transmitted in this e-mail is >> intended only for the person or entity to which it is addressed and may >> contain confidential and/or privileged material. Any review, >> retransmission, dissemination or other use of or taking of any action in >> reliance upon this information by persons or entities other than the >> intended recipient is prohibited. If you received this e-mail in error, >> please contact the sender and delete the material from any computer. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Diana.Martinez-Longoria at ecrmc.org Thu May 18 07:50:04 2017 From: Diana.Martinez-Longoria at ecrmc.org (Diana Martinez-Longoria) Date: Thu, 18 May 2017 12:50:04 +0000 Subject: [Histonet] Decal Solution Message-ID: <12B71261212BE94BB9FB7735484B9FA101014B8652@EXMBX01.ecrmc.ci.el-centro.ca.us> Good morning everyone, I was wondering if someone can give me advise regarding the best decal solution for bone marrow cores? Our pathologist wants to use a decal solution that has EDTA based for molecular studies (ex: suspected tumor/lymphoma etc. diagnosis). Any advice would be greatly appreciated. Thank you! Diana Martinez-Longoria Bachelors of Science in Biology Histotechnician (ASCP)cm El Centro Regional Medical Center Phone: 760-339-7267 Fax: 760-339-4570 Email:dmlongoria at ecrmc.org ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? From dunatrsd at sbcglobal.net Thu May 18 09:25:21 2017 From: dunatrsd at sbcglobal.net (dusko trajkovic) Date: Thu, 18 May 2017 14:25:21 +0000 (UTC) Subject: [Histonet] Histo job opportunity in a GI lab-Oak Lawn Illionios References: <1790045521.669207.1495117521217.ref@mail.yahoo.com> Message-ID: <1790045521.669207.1495117521217@mail.yahoo.com> If anyone is interested in a histology position in a GI lab, please let me know as soon as possible?Lab is located in Oak Lawn Illinois.At the moment I do not have details, but will be getting them soon.ThanksDusko Trajkovic760-390-3478 From plucas at biopath.org Thu May 18 11:54:56 2017 From: plucas at biopath.org (Paula) Date: Thu, 18 May 2017 09:54:56 -0700 Subject: [Histonet] Grosser Needed in Southern California Message-ID: <004601d2cff7$7c1701c0$74450540$@biopath.org> Hello, We need a true PA (Pathologist Assistant) or a highly qualified Grossing person that can do hospital work. Full time or Part Time options. If interested, please contact Jason Cochran (Administrator) for more information. Bio-Path Medical Group Fountain Valley, CA 92708 714-433-1330 Thank you, Paula From KSimeone at leavittmgt.com Thu May 18 12:34:28 2017 From: KSimeone at leavittmgt.com (Kari Simeone) Date: Thu, 18 May 2017 17:34:28 +0000 Subject: [Histonet] A Question About Paraffin Times Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CC91CFA87@vm-email.leavittmgt.com> I agree with Tony. I have on many occasions left the block baskets sit at room temperature (sat them in an OFF oven) and then turned the oven on to temp and re-embedded the tissue with no issues. You could also just return the basket to the warming tray until they are warm again to embed. Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor ________________________________________ From: histonet-request at lists.utsouthwestern.edu [histonet-request at lists.utsouthwestern.edu] Sent: Thursday, May 18, 2017 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 162, Issue 17 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=MuCvzfpBOiQBTWTXYIKZzJY-R5-O_tiGGpyaSEU7wy8&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Histotech and Histology Supervisor job openings in Birmingham, AL (Brannon Owens) 2. Re: A Question About Paraffin Times (Tony Henwood (SCHN)) 3. Re: A Question About Paraffin Times (Garrey Faller) 4. Decal Solution (Diana Martinez-Longoria) 5. Histo job opportunity in a GI lab-Oak Lawn Illionios (dusko trajkovic) 6. Grosser Needed in Southern California (Paula) ---------------------------------------------------------------------- Message: 1 Date: Wed, 17 May 2017 19:40:39 +0000 From: Brannon Owens To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Histotech and Histology Supervisor job openings in Birmingham, AL Message-ID: Content-Type: text/plain; charset="us-ascii" We are currently seeking candidates for several openings in the Birmingham, AL area. Relocation assistance is offered! Position 1: Histology Supervisor (3rd shift) Position 2: Histotechnician (1st shift) Position 3: Histotechnician (3rd shift) Full job descriptions available on the Allied Search Partners website as well as additional opportunities in the field of histology. We look forward to hearing from you! Thank you, To view a complete list of Allied Search Partners current openings go to: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.alliedsearchpartners.com_careers_&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=OYAKUwWjo2B60ZnbVZNPcYLy-11xcbih2bPn7znIoII&e= Brannon Owens VP/Director of Recruitment Allied Search Partners LinkedIn: https://urldefense.proofpoint.com/v2/url?u=https-3A__www.linkedin.com_in_jbrannonowens_&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=zbxgUl7AXC4yU7kuDZDyb5271_RwiU2nRuQQCs_vNkQ&e= https://urldefense.proofpoint.com/v2/url?u=http-3A__www.alliedsearchpartners.com&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=1k7ul6M58-fTSS7jKYGsyX96kDavLFMZIplAdFFSxmw&e= T: 888.388.7571 ext. 106 Direct Line: 407.413.9421 F: 888.388.7572 ------------------------------ Message: 2 Date: Wed, 17 May 2017 23:26:24 +0000 From: "Tony Henwood (SCHN)" To: P Sicurello Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] A Question About Paraffin Times Message-ID: <0237449DE79DBC45B686AB82CDCD16FF95554CC0 at SVDCMBX-MEX008.nswhealth.net> Content-Type: text/plain; charset="us-ascii" It is best to remove the blocks and let them set at room temperature. Leaving them at 60 or more degrees can adversely affect antigens. We routinely do this with our autopsies and have not noted any issues. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 17 May 2017 1:34 AM To: HistoNet Subject: [Histonet] A Question About Paraffin Times Good Morning Listers, I am asking the collective wisdom of the Histonet this question: Is it better to remove baskets from the processor on Saturday morning and: A. Let the cassettes freeze, then melt them down and embed Monday morning? OR B. Leave the cassettes in molten paraffin and embed Monday morning? I am of the opinion that leaving the samples (not fatty, like breast cores) in molten paraffin (62 degrees C) is bad practice, and causes them to get "crunchy", among other things. What do you think? Thank in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=MuCvzfpBOiQBTWTXYIKZzJY-R5-O_tiGGpyaSEU7wy8&e= This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ------------------------------ Message: 3 Date: Thu, 18 May 2017 07:23:50 -0400 From: Garrey Faller To: Cindy Bird Cc: "Logan, Shannon" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] A Question About Paraffin Times Message-ID: Content-Type: text/plain; charset=us-ascii I have had to deal with this in the past and could not find an answer . My intuition was that it can't be good to leave in hot wax. So, on the rare occasion when my only Histotech called in sick I had to take the blocks out and cool them down. When ready they were put back in the tissue embedder to heat up and everything was fine. That's my experience, Garrey Sent from my iPhone > On May 16, 2017, at 2:48 PM, Cindy Bird via Histonet wrote: > > I agree? > > Sent from my iPhone > >> On May 16, 2017, at 1:28 PM, Logan, Shannon via Histonet wrote: >> >> Hello Paula, >> So why must the cassettes be removed on Saturday morning if you aren't embedding until Monday morning? >> Don't you have a "delay start" feature on your processor? Neither option A or B seem like a good thing for the tissue! >> We time our processor to finish at 5 AM Monday when the first Histotech arrives for embedding. >> The cassettes remain in formalin until the processor starts up on Sunday evening. >> >> Regards, >> >> >> Shannon H. Logan B.S., HTL (ASCP) >> Pathology Department >> >> Bellin Health Memorial Hospital >> 744 South Webster Avenue >> Green Bay, WI 54305-3400 >> 920-433-3653 X3727 >> >> >> >> From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] >> Sent: Tuesday, May 16, 2017 10:34 AM >> To: HistoNet >> Subject: [Histonet] A Question About Paraffin Times >> >> Good Morning Listers, >> >> I am asking the collective wisdom of the Histonet this question: >> >> >> Is it better to remove baskets from the processor on Saturday morning and: >> >> A. Let the cassettes freeze, then melt them down and embed Monday morning? >> OR >> B. Leave the cassettes in molten paraffin and embed Monday morning? >> >> >> I am of the opinion that leaving the samples (not fatty, like breast cores) >> in molten paraffin (62 degrees C) is bad practice, and causes them to get >> "crunchy", among other things. >> >> What do you think? >> >> Thank in advance. >> >> Sincerely, >> >> Paula Sicurello, HTL (ASCP)CM >> >> Histotechnology Specialist >> >> UC San Diego Health >> >> 200 Arbor Drive >> >> San Diego, CA 92103 >> >> (P): 619-543-2872 <#> >> >> >> >> *Confidentiality Notice*: The information transmitted in this e-mail is >> intended only for the person or entity to which it is addressed and may >> contain confidential and/or privileged material. Any review, >> retransmission, dissemination or other use of or taking of any action in >> reliance upon this information by persons or entities other than the >> intended recipient is prohibited. If you received this e-mail in error, >> please contact the sender and delete the material from any computer. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=MuCvzfpBOiQBTWTXYIKZzJY-R5-O_tiGGpyaSEU7wy8&e= >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=MuCvzfpBOiQBTWTXYIKZzJY-R5-O_tiGGpyaSEU7wy8&e= > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=MuCvzfpBOiQBTWTXYIKZzJY-R5-O_tiGGpyaSEU7wy8&e= ------------------------------ Message: 4 Date: Thu, 18 May 2017 12:50:04 +0000 From: Diana Martinez-Longoria To: 'Histonet' Subject: [Histonet] Decal Solution Message-ID: <12B71261212BE94BB9FB7735484B9FA101014B8652 at EXMBX01.ecrmc.ci.el-centro.ca.us> Content-Type: text/plain; charset="iso-8859-1" Good morning everyone, I was wondering if someone can give me advise regarding the best decal solution for bone marrow cores? Our pathologist wants to use a decal solution that has EDTA based for molecular studies (ex: suspected tumor/lymphoma etc. diagnosis). Any advice would be greatly appreciated. Thank you! Diana Martinez-Longoria Bachelors of Science in Biology Histotechnician (ASCP)cm El Centro Regional Medical Center Phone: 760-339-7267 Fax: 760-339-4570 Email:dmlongoria at ecrmc.org ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? ------------------------------ Message: 5 Date: Thu, 18 May 2017 14:25:21 +0000 (UTC) From: dusko trajkovic To: "Histonet at lists.utsouthwestern.edu" Subject: [Histonet] Histo job opportunity in a GI lab-Oak Lawn Illionios Message-ID: <1790045521.669207.1495117521217 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 If anyone is interested in a histology position in a GI lab, please let me know as soon as possible?Lab is located in Oak Lawn https://urldefense.proofpoint.com/v2/url?u=http-3A__Illinois.At&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=thbNW8AAFpx_oiEuo4V1LLh3gMir1ia2MinJ-3muB60&e= the moment I do not have details, but will be getting them soon.ThanksDusko Trajkovic760-390-3478 ------------------------------ Message: 6 Date: Thu, 18 May 2017 09:54:56 -0700 From: "Paula" To: Subject: [Histonet] Grosser Needed in Southern California Message-ID: <004601d2cff7$7c1701c0$74450540$@biopath.org> Content-Type: text/plain; charset="us-ascii" Hello, We need a true PA (Pathologist Assistant) or a highly qualified Grossing person that can do hospital work. Full time or Part Time options. If interested, please contact Jason Cochran (Administrator) for more information. Bio-Path Medical Group Fountain Valley, CA 92708 714-433-1330 Thank you, Paula ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=MNlajosbHUc3yQ-IwqtV53ZqEweAbu6iJAOfGUsPVT4&s=MuCvzfpBOiQBTWTXYIKZzJY-R5-O_tiGGpyaSEU7wy8&e= ------------------------------ End of Histonet Digest, Vol 162, Issue 17 *****************************************The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From NMargaryan at luriechildrens.org Thu May 18 13:26:14 2017 From: NMargaryan at luriechildrens.org (Margaryan, Naira) Date: Thu, 18 May 2017 18:26:14 +0000 Subject: [Histonet] PD-L1 and TGFb for FFPE human tissue Message-ID: <34B2EDA118548A4EB35D6E650345BA6469C5E4B3@SV-EX08.childrensmemorial.org> Good morning everyone, I was wondering if anyone can give me an advise regarding the best Abs for the PD-L1 and TGFb for FFPE human tissue (except DAKO Abs, please). Thanks in advance, Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) From mtoole at dcol.net Thu May 18 16:30:41 2017 From: mtoole at dcol.net (Mike Toole) Date: Thu, 18 May 2017 16:30:41 -0500 Subject: [Histonet] Adhesive cover for microscope state. In-Reply-To: <6347C6D2B080534F9B5C2B08436DCFAF113BC261@COLOEXCH01.uropartners.local> References: <6347C6D2B080534F9B5C2B08436DCFAF113BC261@COLOEXCH01.uropartners.local> Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB34B3C665BDB@mail> http://bioindustrialproducts.com/wp-content/uploads/catalogs/2017a/toc1_microscopes_and_magnifiers.pdf Microscope Stage Laminate, USA. 16251.................................... $8.00 6.25 x 7.75? sheet provided with a #10 scalpel. White TeflonR (PTFE) on self-adhesive vinyl is 0.010" thick. Mike Toole -----Original Message----- From: Lester Raff MD via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 09, 2017 8:21 AM To: Histonet (histonet at lists.utsouthwestern.edu) Subject: [EXTERNAL] [Histonet] Adhesive cover for microscope state. Hello All: Years ago I had a white adhesive plastic cover on my microscope stage that kept the stage smooth. I am looking for a similar product but having no luck with an internet search. Does anyone have any leads on this? Thanks, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt at pa-ucl.com Mon May 22 10:11:09 2017 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Mon, 22 May 2017 15:11:09 +0000 Subject: [Histonet] validation for special stains Message-ID: <4ecbe7301fc149d9a6f47dcef44bcc2d@mercury.wad.pa-ucl.com> Good Morning- What is everyone doing for validation on special stains? We have a new Artisan but I am struggling with finding exact validation specifications. It will be difficult to find 10-20 cases on some of these stains. Thoughts and Thank you! Nancy Pathology Support Services Manager United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Erin.Martin at ucsf.edu Mon May 22 10:28:47 2017 From: Erin.Martin at ucsf.edu (Martin, Erin) Date: Mon, 22 May 2017 15:28:47 +0000 Subject: [Histonet] HHV-8 Message-ID: Good morning histonetters! I'm looking for a new source for HHV-8 (KSV), clone 13B10, antibody. CellMarque's is no longer IVD, nor is Ventana. Does anyone have a suggestion? Thank you! Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. From lmarie08 at uga.edu Mon May 22 10:40:32 2017 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Mon, 22 May 2017 15:40:32 +0000 Subject: [Histonet] blades Message-ID: Hi Histoland- Blades for grossing tissues- anyone have recommendations? The vendor that we were purchasing just straight razor blades from has stopped manufacturing them. We don't use scalpels because of their higher cost and we are only working with poultry samples. Thanks and have a great day! From pdefazio802 at gmail.com Mon May 22 12:34:10 2017 From: pdefazio802 at gmail.com (Pam DeFazio) Date: Mon, 22 May 2017 17:34:10 +0000 Subject: [Histonet] Histonet Digest, Vol 162, Issue 19 In-Reply-To: References: Message-ID: We use "carpet blades" from Lowes or Home Depot. They are cheap and fit the handles we already have On Mon, May 22, 2017 at 1:13 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. validation for special stains (Nancy Schmitt) > 2. HHV-8 (Martin, Erin) > 3. blades (Lauren Sweeney) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 22 May 2017 15:11:09 +0000 > From: Nancy Schmitt > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: [Histonet] validation for special stains > Message-ID: <4ecbe7301fc149d9a6f47dcef44bcc2d at mercury.wad.pa-ucl.com> > Content-Type: text/plain; charset="us-ascii" > > Good Morning- > > What is everyone doing for validation on special stains? We have a new > Artisan but I am struggling with finding exact validation specifications. > It will be difficult to find 10-20 cases on some of these stains. > > Thoughts and Thank you! > > Nancy > Pathology Support Services Manager > United Clinical Laboratories > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > > ------------------------------ > > Message: 2 > Date: Mon, 22 May 2017 15:28:47 +0000 > From: "Martin, Erin" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] HHV-8 > Message-ID: > < > DM2PR05MB574ACB887A0A7A579A9885090F80 at DM2PR05MB574.namprd05.prod.outlook.com > > > > Content-Type: text/plain; charset="iso-8859-1" > > Good morning histonetters! > > > I'm looking for a new source for HHV-8 (KSV), clone 13B10, antibody. > CellMarque's is no longer IVD, nor is Ventana. Does anyone have a > suggestion? > > > Thank you! > > Erin Martin, Histology Supervisor > > UCSF Dermatopathology and Oral Pathology Service > 1701 Divisadero St, San Francisco, CA 94044 > 415-353-7248 > > Confidentiality Notice > The information transmitted is intended only for the person or entity to > which it is addressed and may contain confidential and/or privileged > material. Any review, retransmission, dissemination or other use of, or > taking of any action in reliance upon, this information by persons or > entities other than the intended recipient is prohibited. If you receive > this in error please contact the sender and delete the material from any > computer. > > > ------------------------------ > > Message: 3 > Date: Mon, 22 May 2017 15:40:32 +0000 > From: Lauren Sweeney > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] blades > Message-ID: > < > CY4PR02MB2808EEE752BB38BB4F4E8FE0C5F80 at CY4PR02MB2808.namprd02.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > Hi Histoland- > > Blades for grossing tissues- anyone have recommendations? The vendor that > we were purchasing just straight razor blades from has stopped > manufacturing them. We don't use scalpels because of their higher cost and > we are only working with poultry samples. > > Thanks and have a great day! > > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 162, Issue 19 > ***************************************** > From tbraud at holyredeemer.com Mon May 22 12:42:44 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 22 May 2017 17:42:44 +0000 Subject: [Histonet] Stain Validations Message-ID: <48E053DDF6CE074DB6A7414BA05403F81FDC9B@HRHEX03-HOS.holyredeemer.local> Just use a microarray. They can be purchased in all sorts of varieties with hundreds of cases on one slide. I use and recommend U S Biomax Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. validation for special stains (Nancy Schmitt) 2. HHV-8 (Martin, Erin) 3. blades (Lauren Sweeney) ---------------------------------------------------------------------- Message: 1 Date: Mon, 22 May 2017 15:11:09 +0000 From: Nancy Schmitt Subject: [Histonet] validation for special stains Good Morning- What is everyone doing for validation on special stains? We have a new Artisan but I am struggling with finding exact validation specifications. It will be difficult to find 10-20 cases on some of these stains. Thoughts and Thank you! Nancy Pathology Support Services Manager United Clinical Laboratories From rsrichmond at gmail.com Mon May 22 12:50:47 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Mon, 22 May 2017 13:50:47 -0400 Subject: [Histonet] blades Message-ID: Lauren Sweeney at the University of Georgia asks: >>Blades for grossing tissues- anyone have recommendations? The vendor that we were purchasing just straight razor blades from has stopped manufacturing them. We don't use scalpels because of their higher cost and we are only working with poultry samples.<< I just Googled "single edge razor blades" and they're still available from a number of sources. I've done hospital pathology grossing with them, though not in the last 40 years, and found them extremely satisfactory - in fact, I prefer them to scalpel blades. I've been shaving my face since 1951, and have never heard of anybody actually using them to shave with. Bob Richmond gray bearded samurai pathologist Maryville TN From tkngflght at yahoo.com Mon May 22 14:40:13 2017 From: tkngflght at yahoo.com (Cheryl) Date: Mon, 22 May 2017 19:40:13 +0000 (UTC) Subject: [Histonet] Still looking for a Lead Tech for my happy little reference lab - In-Reply-To: References: Message-ID: <1449426457.3471189.1495482013610@mail.yahoo.com> Hi Guys- Seeking a registered HT with at least 5 years of clinical bench experience. ?Grossing eligible (we'll teach if you haven't learned it yet) Give me a shout for more details!?Cheryl Kerry, HT(ASCP)?ADG Pathology Houston TX ckerry at adgpath.com ? From SteveM at mcclainlab.com Tue May 23 12:29:24 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Tue, 23 May 2017 17:29:24 +0000 Subject: [Histonet] Blades Message-ID: <9C760E5C-4270-4E14-98B5-6914B7F94986@mcclainlab.com> http://www.emsdiasum.com/microscopy/products/preparation/blades.aspx Shared via the Google app Try electron microscopy sciences. They have a variety of blades. Steve A. McClain, MD From Richard.Cartun at hhchealth.org Tue May 23 13:32:43 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 23 May 2017 18:32:43 +0000 Subject: [Histonet] IHC testing for Parvovirus (Human) Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E95438E7C@HHCEXCHMB03.hhcsystem.org> If you do this test, where do you get your antibody? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From tony.henwood at health.nsw.gov.au Tue May 23 18:58:34 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue, 23 May 2017 23:58:34 +0000 Subject: [Histonet] IHC testing for Parvovirus (Human) In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E95438E7C@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E95438E7C@HHCEXCHMB03.hhcsystem.org> Message-ID: <0237449DE79DBC45B686AB82CDCD16FF95556064@SVDCMBX-MEX008.nswhealth.net> Hi Richard, We use NCL-PARVO from Leica Details as follows: ANTIGEN NAME: Parvovirus B19 OTHER NAMES: Clone: R92F6 Isotype: IgG1 DESCRIPTION: Mouse monoclonal to native parvovirus B19 purified from human plasma. Human parvovirus B19 (VP1 and VP2 capsid proteins). STORAGE CONDITIONS: 4-8oC undiluted SUPPLIER: Leica NCL-PARVO PROCEDURE: Methodology: HRP-POLYMER Working Dilution: Bond: 1/100 Special Conditions: Citrate HIER required (ER1) CONTROL TISSUE: Placenta containing Parvovirus Inbuilt Controls CLINICAL SIGNIFICANCE: Diagnosis of parvovirus B19 infection in formalin-fixed, paraffin embedded tissue can be made through the identification of the characteristic brick-red intranuclear inclusions in normoblasts within the fetal circulation in histological sections stained with HE. It has been found that immunohistochemistry was the best detection method. It is highly specific and sensitive, preserves the morphology and reveals a larger number of positive cells than does HE with the advantage of showing cytoplasmic and nuclear positivity, making it more reliable. REFERENCES: Li, J. J., Henwood, T., Van Hal, S., & Charlton, A. (2015). Parvovirus infection: an immunohistochemical study using fetal and placental tissue. Pediatric and Developmental Pathology, 18(1), 30-39. Quemelo PRV, Lima DM, Fonseca BAL, Peres LC (2007) "Detection of parvovirus B19 infection in formalin-fixed and paraffin-embedded placenta and fetal tissues" Rev. Inst. Med. trop. S. Paulo, 49(2): 103-107, Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 24 May 2017 4:33 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC testing for Parvovirus (Human) If you do this test, where do you get your antibody? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From funderwood at mcohio.org Wed May 24 08:02:39 2017 From: funderwood at mcohio.org (Underwood, Fred) Date: Wed, 24 May 2017 13:02:39 +0000 Subject: [Histonet] heated block trimmers Message-ID: Hi All, Anyone have any experience with the heated block trimmers? Specifically, I was looking at the one from Newcomer. Thanks, Fred From liz at premierlab.com Wed May 24 10:17:38 2017 From: liz at premierlab.com (Elizabeth Chlipala) Date: Wed, 24 May 2017 09:17:38 -0600 Subject: [Histonet] heated block trimmers In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE0302C9EF8749@SBS2K8.premierlab.local> Fred We have a block trimmer here in the lab it's not the newcomer supply its from Shandon, we like it. I personally do not use it but the rest of the technicians do. I'm a bit old school I have a very old steak knife that I have been using since 1996 so it's nice and dull and works perfectly for trimming excess paraffin off blocks. What's nice about these trimmers is that they decrease the repetitive motion of scraping the block with a knife, so they are better overall. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Underwood, Fred via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, May 24, 2017 7:03 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] heated block trimmers Hi All, Anyone have any experience with the heated block trimmers? Specifically, I was looking at the one from Newcomer. Thanks, Fred _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ From Toni.McDaniel at ky.gov Wed May 24 10:31:23 2017 From: Toni.McDaniel at ky.gov (McDaniel, Toni (Justice)) Date: Wed, 24 May 2017 15:31:23 +0000 Subject: [Histonet] Block trimmer questions Message-ID: <5D09742D8BBF254588268A6C35B92CE2708ED8E3@AGEXM01.eas.ds.ky.gov> I have a TBS shur/trim and I love it compared to 2 others I have used. It has a large surface and its Teflon so it does not tear up the blocks. What can't be heat trimmed is the writing portion of the block with the case number and scan portion if this is the method used in your lab. Hope my input helps. Toni McDaniel Forensic Histotech Specialist HT, ASCP Office of the Chief Medical Examiner 10511 LaGrange Road Bingham Bldg. 1st floor Louisville, KY 40223 off: 502-489-5209 fax: 502-489-5213 From Michelle.Dragoo at nm.org Wed May 24 12:07:24 2017 From: Michelle.Dragoo at nm.org (Dragoo, Michelle) Date: Wed, 24 May 2017 17:07:24 +0000 Subject: [Histonet] heated block trimmers In-Reply-To: References: Message-ID: <11bfb48dc30641738099c4353045abf2@exc0i44wp.ch.cadhlt.org> Hi Fred, We use the ones from Thermo Fisher and are quite happy with them. Michelle R. Dragoo, MBA, HT (ASCP) Supervisor, Histology Northwestern Medicine Central Dupage Hospital 25 North Winfield Road, Winfield IL 60190 O: 630.933.6748 michelle.dragoo at nm.org -----Original Message----- From: Underwood, Fred [mailto:funderwood at mcohio.org] Sent: Wednesday, May 24, 2017 8:03 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] heated block trimmers Hi All, Anyone have any experience with the heated block trimmers? Specifically, I was looking at the one from Newcomer. Thanks, Fred This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From patpxs at gmail.com Wed May 24 12:07:45 2017 From: patpxs at gmail.com (P Sicurello) Date: Wed, 24 May 2017 10:07:45 -0700 Subject: [Histonet] Adenovirus Control Block Source? Message-ID: Good Morning Listers, Does anyone have a source for a good Adenovirus control block for IHC? Thank you in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From Julie at seascapesurgerycenter.com Wed May 24 13:50:34 2017 From: Julie at seascapesurgerycenter.com (Julie Bowman) Date: Wed, 24 May 2017 18:50:34 +0000 Subject: [Histonet] Histotech position open in the Tampa Bay area! Message-ID: Our state-of-the-art dermatologic surgery practice in New Tampa is seeking a motivated candidate with professionalism, attention to detail, and a strong work ethic to join our team! Beautiful work environment and great benefits. Responsibilities include?Mohs specimen (frozen section) preparation as well as permanent section preparation. Experience with Mohs and/or immunohistochemistry isn?t required, but is a plus! Monday through Friday position with hours worked falling between 8am and 6pm Please send resumes to Julie at seascapesurgerycenter.com From JMacDonald at mtsac.edu Wed May 24 14:03:28 2017 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Wed, 24 May 2017 12:03:28 -0700 Subject: [Histonet] heated block trimmers In-Reply-To: References: Message-ID: We have two of the Paratrimmers by Shandon. They really cut down on the mess with scraping the blocks and the repetitive motion. From: "Underwood, Fred via Histonet" To: "histonet at lists.utsouthwestern.edu" Date: 05/24/2017 06:04 AM Subject: [Histonet] heated block trimmers Hi All, Anyone have any experience with the heated block trimmers? Specifically, I was looking at the one from Newcomer. Thanks, Fred _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From john.frazier at roche.com Wed May 24 16:57:18 2017 From: john.frazier at roche.com (Frazier, John) Date: Wed, 24 May 2017 17:57:18 -0400 Subject: [Histonet] heated block trimmers Message-ID: <-8104464780030218336@unknownmsgid> The heated block trimmers are great for labs with a Bar Code tracking system. The heater will not impede the integrity of the Bar Code on the block in the process of removing paraffin, like scraping the block can do. Additionally, most people can de-wax multiple blocks at one time. The Shandon block heater is the one I have seen in many labs. You may want to check out EBay and or a company that sales used medical equipment. Sent from my iPad Sent from my iPad > On May 24, 2017, at 9:02 AM, Underwood, Fred wrote: > > Hi All, > > Anyone have any experience with the heated block trimmers? Specifically, I was looking at the one from Newcomer. > > Thanks, > Fred > From tony.henwood at health.nsw.gov.au Wed May 24 18:17:54 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 24 May 2017 23:17:54 +0000 Subject: [Histonet] heated block trimmers In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF955563BE@SVDCMBX-MEX008.nswhealth.net> Yep, We have one and our new embedding centre has one built in. Saves a lot of time in preparing blocks for trimming. A lot safer than using a knife/scraper to remove excess wax from the block. Highly recommend it Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Underwood, Fred via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 24 May 2017 11:03 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] heated block trimmers Hi All, Anyone have any experience with the heated block trimmers? Specifically, I was looking at the one from Newcomer. Thanks, Fred _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From thomasebrooks at aol.com Wed May 24 18:33:52 2017 From: thomasebrooks at aol.com (thomasebrooks at aol.com) Date: Wed, 24 May 2017 19:33:52 -0400 Subject: [Histonet] heated block trimmers In-Reply-To: References: Message-ID: <15c3ccf3a2a-324a-11986@webprd-m50.mail.aol.com> Fred I recently found it necessary to purchase a paraffin block trimmer and was surprised to find that the ThermoShandon has been discontinued. We purchased the Newcomer andthe techs rarely touch the old Shandon Paratrimmers. The unit has two power settings but the "low" setting is more than sufficient for our needs. Thomas Brooks -----Original Message----- From: Underwood, Fred via Histonet To: histonet Sent: Wed, May 24, 2017 8:19 am Subject: [Histonet] heated block trimmers Hi All, Anyone have any experience with the heated block trimmers? Specifically, I was looking at the one from Newcomer. Thanks, Fred _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From funderwood at mcohio.org Thu May 25 07:45:39 2017 From: funderwood at mcohio.org (Underwood, Fred) Date: Thu, 25 May 2017 12:45:39 +0000 Subject: [Histonet] heated block trimmers In-Reply-To: <15c3ccf3a2a-324a-11986@webprd-m50.mail.aol.com> References: <15c3ccf3a2a-324a-11986@webprd-m50.mail.aol.com> Message-ID: Thank you everyone for the fantastic feedback! I?m considering one of these due ligament issues with my thumbs. I suspect 30+ years of scraping blocks may be a contributing factor. Thanks again, Fred From: thomasebrooks at aol.com [mailto:thomasebrooks at aol.com] Sent: Wednesday, May 24, 2017 7:34 PM To: Underwood, Fred; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] heated block trimmers Fred I recently found it necessary to purchase a paraffin block trimmer and was surprised to find that the ThermoShandon has been discontinued. We purchased the Newcomer andthe techs rarely touch the old Shandon Paratrimmers. The unit has two power settings but the "low" setting is more than sufficient for our needs. Thomas Brooks -----Original Message----- From: Underwood, Fred via Histonet > To: histonet > Sent: Wed, May 24, 2017 8:19 am Subject: [Histonet] heated block trimmers Hi All, Anyone have any experience with the heated block trimmers? Specifically, I was looking at the one from Newcomer. Thanks, Fred _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kelly.Pairan at nationwidechildrens.org Thu May 25 11:29:43 2017 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Thu, 25 May 2017 16:29:43 +0000 Subject: [Histonet] Inhibin Message-ID: <9e45802715264024917187717bd9b27b@l1perdwmbx01.childrensroot.net> Hi Everyone, I went to order more Inhibin yesterday from our current supplier and that antibody has been discontinued. Are you running this antibody in your lab and if so, which supplier do you use? Also, does anyone use Dianova antibodies? We have one from this company but I cannot seem to locate a distributer in this country. Thanks, Kelly From jdhannasch at gmail.com Thu May 25 12:40:17 2017 From: jdhannasch at gmail.com (jdhannasch at gmail.com) Date: Thu, 25 May 2017 10:40:17 -0700 Subject: [Histonet] Coagulants question Message-ID: Can someone explain to me what a coagulant vs a noncoagulant is and why it matters? I have never been able to really understand coagulants and why they are important. Thanks in advance From tony.henwood at health.nsw.gov.au Thu May 25 19:17:11 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 26 May 2017 00:17:11 +0000 Subject: [Histonet] Inhibin In-Reply-To: <9e45802715264024917187717bd9b27b@l1perdwmbx01.childrensroot.net> References: <9e45802715264024917187717bd9b27b@l1perdwmbx01.childrensroot.net> Message-ID: <0237449DE79DBC45B686AB82CDCD16FF95556756@SVDCMBX-MEX008.nswhealth.net> We use Leica rtu PA0110 on the Bond 3 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Pairan, Kelly via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 26 May 2017 2:30 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Inhibin Hi Everyone, I went to order more Inhibin yesterday from our current supplier and that antibody has been discontinued. Are you running this antibody in your lab and if so, which supplier do you use? Also, does anyone use Dianova antibodies? We have one from this company but I cannot seem to locate a distributer in this country. Thanks, Kelly _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From Kelly.Pairan at nationwidechildrens.org Fri May 26 12:22:08 2017 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Fri, 26 May 2017 17:22:08 +0000 Subject: [Histonet] TTG Message-ID: <637fe894627b4c6d806fbc7f7610d706@l1perdwmbx01.childrensroot.net> Happy Friday! A special thank you to everyone who answered by question about Inhibin yesterday. One of my pathologists has now asked me to reach out to all of you to ask if anyone is using TTG for Duodenal Biopsies from possible Celiac patients. If so, where are you currently getting your antibody and do you have any problems with the staining? Thank you for your help and have a lovely long weekend! Kelly From sswartwood at chla.usc.edu Fri May 26 13:30:28 2017 From: sswartwood at chla.usc.edu (Swartwood, Steven) Date: Fri, 26 May 2017 18:30:28 +0000 Subject: [Histonet] Non specific binding to mouse macrophages/lymphocytes Message-ID: Hello all, I'm staining mouse lungs and heart with a mouse hosted primary antibody made by a research lab so I don't have much wiggle room with the primary antibody. I'm using a MOM kit for IHC staining. The only background I can't seem to get rid of is macrophages or lymphocytes. Would an Fc receptor block help on mouse tissues? Does anyone have a good Fc receptor block they use and would recommend on mouse tissues? Another step I can try is to get a secondary antibody that's a F(ab)2, but does anyone have any input about Fc blocking on mouse tissues? Steven Swartwood HT (ASCP) Anatomic Pathology Children's Hospital Los Angeles CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From naje1972 at yahoo.com Fri May 26 13:45:56 2017 From: naje1972 at yahoo.com (cynthia haynes) Date: Fri, 26 May 2017 18:45:56 +0000 (UTC) Subject: [Histonet] TTG In-Reply-To: <637fe894627b4c6d806fbc7f7610d706@l1perdwmbx01.childrensroot.net> References: <637fe894627b4c6d806fbc7f7610d706@l1perdwmbx01.childrensroot.net> Message-ID: <1213772335.878253.1495824356256@mail.yahoo.com> My pathologist usually asks for CD68 to be done on possible celiac cases.Cynthia Haynes-James H.T? Sent from Yahoo Mail on Android On Fri, May 26, 2017 at 12:35 PM, Pairan, Kelly via Histonet wrote: Happy Friday! A special thank you to everyone who answered by question about Inhibin yesterday.? One of my pathologists has now asked me to reach out to all of you to ask if anyone is using TTG for Duodenal Biopsies from possible Celiac patients.? If so, where are you currently getting your antibody and do you have any problems with the staining? Thank you for your help and have a lovely long weekend! Kelly _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Sat May 27 09:22:09 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 27 May 2017 10:22:09 -0400 Subject: [Histonet] Coagulants question Message-ID: From: jdhannasch at gmail.com To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Coagulants question Message-ID: Content-Type: text/plain; charset=us-ascii "jdhannasch" (who) asks: >>Can someone explain to me what a coagulant vs a noncoagulant is and why it matters? I have never been able to really understand coagulants and why they are important.<< I suppose you're referring to fixatives. Coagulant fixatives make blood clot, often because of their acidity. Neutral buffered formalin (NBF) doesn't clot blood. Back when I was doing bone marrow aspirations and biopsies in the 1970s, I'd squirt some of the unclotted aspirate into NBF, and get small marrow particles suspended in liquid with red blood cells. I'd filter out the liquid part, then have the loose particles embedded. The rest I'd put in Zenker/Helly fixative (those were the days, my friend!) where it would promptly clot. I'd get the iron stain done on the particle suspension, where the hemosiderin hadn't been leached out by the acid fixative or by the decalcifier. For further information, consult one of the textbooks like John Kiernan's. Bob Richmond Samurai Pathologist Maryville TN From Rhonda.Gregoire at gov.mb.ca Mon May 29 10:04:14 2017 From: Rhonda.Gregoire at gov.mb.ca (Gregoire, Rhonda (AGR)) Date: Mon, 29 May 2017 15:04:14 +0000 Subject: [Histonet] Butterfly histology Message-ID: <4bc5379c797d4b21b354e8cffb5e3d8c@CAWNSMBME106.ME.MBGOV.CA> Does anyone have any experience processing and paraffin embedding butterflies? Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture 545 University Crescent, Winnipeg, MB, R3T 5S6 Rhonda.Gregoire at gov.mb.ca T : 204-945-7641 F : 204-945-7646 From koellingr at comcast.net Mon May 29 10:46:19 2017 From: koellingr at comcast.net (koellingr at comcast.net) Date: Mon, 29 May 2017 15:46:19 +0000 (UTC) Subject: [Histonet] Much off from normal histology topics In-Reply-To: <1850684785.39912886.1496071442500.JavaMail.zimbra@comcast.net> Message-ID: <1145730196.39929829.1496072779818.JavaMail.zimbra@comcast.net> So please delete as quickly as you want. Happy Memorial Day and everlasting thanks to those who gave their all. Just returned last week from the International Science and Engineering Fair in Los Angeles. 1,700 high school students from 75 countries and regions with their 1,400 projects. Not only were there numerous medical, pathology, biotech or biological research projects, a couple were utilizing classical histology . I chaperoned two kids from high school in Spokane to join the entire 19 student "Team Washington". We (Team Washington students) won some individual prizes. But there were many projects around the massive hall concerning B&T cells, dendritic cells, melanoma diagnosis, PCR, BRCA1 and 2 genes and on and on and literally on about "tissues" if I wanted to keep typing. There is an Internationally ISEF affiliated state fair in ALMOST every state. And countries all over the world including two counties from Africa, there for the first time. Work with a high schooler and through a doctor, hospital, lab, or educational institute on a histology project. It can be done if. Ray Koelling Lecturer, University of Washington School of Medicine, WWAMI Spokane From cforster at umn.edu Tue May 30 11:52:50 2017 From: cforster at umn.edu (Colleen Forster) Date: Tue, 30 May 2017 11:52:50 -0500 Subject: [Histonet] Butterfly histology In-Reply-To: <4bc5379c797d4b21b354e8cffb5e3d8c@CAWNSMBME106.ME.MBGOV.CA> References: <4bc5379c797d4b21b354e8cffb5e3d8c@CAWNSMBME106.ME.MBGOV.CA> Message-ID: Following..... Colleen Forster On Mon, May 29, 2017 at 10:04 AM, Gregoire, Rhonda (AGR) via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Does anyone have any experience processing and paraffin embedding > butterflies? > > Rhonda Gregoire, MLT > Supervisor, Clinical Pathology > Veterinary Diagnostic Services > Manitoba Agriculture > 545 University Crescent, Winnipeg, MB, R3T 5S6 > Rhonda.Gregoire at gov.mb.ca > T : 204-945-7641 F : 204-945-7646 > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From paula at excaliburpathology.com Tue May 30 12:40:34 2017 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Tue, 30 May 2017 17:40:34 +0000 (UTC) Subject: [Histonet] Butterfly Histology References: <205454591.2682498.1496166034950.ref@mail.yahoo.com> Message-ID: <205454591.2682498.1496166034950@mail.yahoo.com> Hi, I do drosophila and other insects. Use an alcoholic fixative such as Davidson's for better infiltration. Transfer to 70% EtOH after 24-48 hours. Normal processing schedule with 30 minutes to an hour starting at 70-80%. 1-1.5 hours each for paraffin.?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From SteveM at mcclainlab.com Wed May 31 05:08:50 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Wed, 31 May 2017 10:08:50 +0000 Subject: [Histonet] Histonet Digest, Vol 162, Issue 26 butterflies In-Reply-To: References: Message-ID: <96109F40-8F2B-410F-9770-BBE41246FB11@mcclainlab.com> I have no experiences w butterflies, but the chitin in ticks, carpenter ants and spiders does not cut well. For insects and arthropods we post-fix in Carnoy's for 60 minutes Then soften with 10% KOH RT for 10-20 minutes then wash for 30-60minutes in water. Long processing cycle. Steve A. McClain, MD On May 30, 2017, at 13:26, "histonet-request at lists.utsouthwestern.edu" wrote: >> Does anyone have any experience processing and paraffin embedding >> butterflies? >> >> Rhonda Gregoire, MLT >> Supervisor, Clinical Pathology >> Veterinary Diagnostic Services >> Manitoba Agriculture >> 545 University Crescent, Winnipeg, MB, R3T 5S6 >> Rhonda.Gregoire at gov.mb.ca >> T : 204-945-7641 F : 204-945-7646 From Dearolf at hendrix.edu Wed May 31 10:53:09 2017 From: Dearolf at hendrix.edu (Dearolf, Jenn) Date: Wed, 31 May 2017 15:53:09 +0000 Subject: [Histonet] Help with preparing slides of baby guppies Message-ID: Hello! My name is Jenn Dearolf, and I am a professor in the Biology Department at Hendrix College, a small liberal arts college in Conway, AR. I have written this list before to get advice about how to prevent freezing artifact in small muscle samples (Thanks!), but today, I have a completely different need. In our Zoology course at Hendrix, we use slides that have numerous serial sections (7 to 10 microns thickness) of a baby guppy on them that have been stained with H & E. And, a box of slides, ranging from 8 to 12 slides, is the entire guppy sectioned from the tip of its nose to the tip of its tail. However, these slides are very old, and over the years, the mounting media has pulled away from the sections. In addition, numerous slides have been broken or lost. Unfortunately, no one in my Department now has the necessary skills to produce these slides for our students. I was wondering if anyone on the list knew of a facility that could produce these slides. I think we should be able to provide the baby guppies. I would just have to get approval from our IACUC. I also have a very old paper that discusses the steps that were necessary to produce the slides. I am happy to share the methodology with anyone that could help us, and we could discuss what we would need to do at Hendrix and which steps would need to be performed at your facility. I appreciate any advice folks are willing to share. Thanks for your time and consideration. Sincerely, Jenn Jennifer Dearolf, Ph.D. Professor and Chair Biology Department Hendrix College 1600 Washington Ave. Conway, AR 72032 (501) 450-4530 (office) From craigak12 at gmail.com Wed May 31 11:50:55 2017 From: craigak12 at gmail.com (J B) Date: Wed, 31 May 2017 16:50:55 +0000 Subject: [Histonet] Help with preparing slides of baby guppies In-Reply-To: References: Message-ID: Jenn, Trying to fully understand. You need someone to prepare these slides again for you? Great quality work, you provide the specimen? Let me know, I would love to learn more. Sincerely, JB On Wed, May 31, 2017, 9:13 AM Dearolf, Jenn via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello! > > My name is Jenn Dearolf, and I am a professor in the Biology Department at > Hendrix College, a small liberal arts college in Conway, AR. I have > written this list before to get advice about how to prevent freezing > artifact in small muscle samples (Thanks!), but today, I have a completely > different need. > > In our Zoology course at Hendrix, we use slides that have numerous serial > sections (7 to 10 microns thickness) of a baby guppy on them that have been > stained with H & E. And, a box of slides, ranging from 8 to 12 slides, is > the entire guppy sectioned from the tip of its nose to the tip of its > tail. However, these slides are very old, and over the years, the mounting > media has pulled away from the sections. In addition, numerous slides have > been broken or lost. > > Unfortunately, no one in my Department now has the necessary skills to > produce these slides for our students. I was wondering if anyone on the > list knew of a facility that could produce these slides. I think we should > be able to provide the baby guppies. I would just have to get approval > from our IACUC. > > I also have a very old paper that discusses the steps that were necessary > to produce the slides. I am happy to share the methodology with anyone > that could help us, and we could discuss what we would need to do at > Hendrix and which steps would need to be performed at your facility. > > I appreciate any advice folks are willing to share. Thanks for your time > and consideration. > > Sincerely, > Jenn > > > Jennifer Dearolf, Ph.D. > Professor and Chair > Biology Department > Hendrix College > 1600 Washington Ave. > Conway, AR 72032 > (501) 450-4530 (office) > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Have a great day! From jcox90 at yahoo.com Wed May 31 11:57:00 2017 From: jcox90 at yahoo.com (Jill Cox) Date: Wed, 31 May 2017 16:57:00 +0000 (UTC) Subject: [Histonet] Looking for used microtome References: <1868781693.4369727.1496249820171.ref@mail.yahoo.com> Message-ID: <1868781693.4369727.1496249820171@mail.yahoo.com> Hi all, I'm looking for a good used microtome preferably Sakura SRM 200 or newer Leica. Hoping to purchase local near Phoenix AZ. It's for a lab I help out part time here. Found a couple on eBay but just want to make sure it is in good working condition. Thank you!!! Jill Cox HT ASCP