From Ana.Maluenda at baker.edu.au Fri Jun 2 03:00:39 2017 From: Ana.Maluenda at baker.edu.au (Ana Maluenda) Date: Fri, 2 Jun 2017 08:00:39 +0000 Subject: [Histonet] IHC for secreted proteins and cytokines in frozen sections - Pre-fix or post-fix? Message-ID: Hi everyone, Just wondering what is people's opinion and protocols for IHC in mouse frozen sections targeting secreted proteins and cytokines. I see lots of places using fresh frozen sections/snap-freeze and cold acetone or methanol/ethanol post-fixation. Is this an issue when it comes to diffusion of such proteins in the tissue, since they are not well localized or linked to cellular structures? Would it be better to pre-fix (either immersion in 4%PFA or infusion with fixative) than post-fix? Any thoughts would be much appreciated. Kind regards, Ana Ana Maluenda Research Assistant Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting privacy at baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg From Timothy.Morken at ucsf.edu Fri Jun 2 11:16:10 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 2 Jun 2017 16:16:10 +0000 Subject: [Histonet] IHC for secreted proteins and cytokines in frozen sections - Pre-fix or post-fix? In-Reply-To: References: Message-ID: Ana, you should fix before sectioning. Chris Van der Loos did an excellent study on loss of cytokines in frozen sections vs whole cells. See his paper: Immunohistochemical Detection of Interferon-y, Fake or Fact? CM Van der Loos, et.al., J Histochem Cytochem, 49(6):699-709, 2001. Www.jhc.org Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Ana Maluenda via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, June 02, 2017 1:01 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC for secreted proteins and cytokines in frozen sections - Pre-fix or post-fix? Hi everyone, Just wondering what is people's opinion and protocols for IHC in mouse frozen sections targeting secreted proteins and cytokines. I see lots of places using fresh frozen sections/snap-freeze and cold acetone or methanol/ethanol post-fixation. Is this an issue when it comes to diffusion of such proteins in the tissue, since they are not well localized or linked to cellular structures? Would it be better to pre-fix (either immersion in 4%PFA or infusion with fixative) than post-fix? Any thoughts would be much appreciated. Kind regards, Ana Ana Maluenda Research Assistant Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting privacy at baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Sat Jun 3 14:22:50 2017 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sat, 3 Jun 2017 19:22:50 +0000 Subject: [Histonet] What happened to Ultraclone/PGP9.5? Message-ID: Ultraclone THE supplier of PGP9.5 for many years ! Respect to them/him/her/LTB No longer....sigh. Ultraclone: "legendary"...chuckle Sure....other Suppliers now offer anti PGP9.5 abs Why then do they still call it "PGP9.5"?? We now know what the protein is. PGP: "Pretty good protein" I recall? Why the 9.5? Usually a clone but, but Ultraclone ab was a rabbit poly. One could only order via fax, I recall. Isle of Wight location: Rossiters Farm. Any more info re Ultraclone's history would be most appreciated. Curiously Carl From cebass at buffalo.edu Sun Jun 4 17:32:32 2017 From: cebass at buffalo.edu (Bass, Caroline) Date: Sun, 4 Jun 2017 22:32:32 +0000 Subject: [Histonet] budget camera for DAB sections on an old olympus BH-2 Message-ID: Hi Everyone, I do a lot of DAB staining on relatively thick rat brain sections (40um). I have been using a really nice slide scanning system for publication quality images. Recently I acquired a really nice Olympus BH-2, it has great objectives pilfered from an old confocal microscope. I?m on the lookout for a used trinocular head so I can take pictures. We have two basic needs: 1) to provide a viewing screen so that more than one person can assess a section, going back and forth between 3-4 people becomes a pain. 2) I?d like to take mid-quality pictures for notebooks and to make decisions on which to image with more quality (so having my students send me pdfs of images). 3) it would be fantastic if I could use some sort of wi-fi system to use with phones/tablets, etc. So we could easily share photos without a computer. 4) cheap! My thought is to go with any one of the super cheap cameras out there that have nice sharing features or buy a used camera that?s decent. I?d like to keep it under $500. I?ve seen used Zeiss color Axiocams, olympus DP70s, 10, 11, 12s, and then various infinity, spot, qimaging and coolsnap cameras. Any suggestions for keeping things inexpensive, but taking decent quality images thats good for archiving, sharing and generally assessing the tissue to decide which section to image for publications? What I don?t know is what it would cost to get any of these used cameras working, do I have to pay for extra cards (firewire, etc), controllers, and software? Thanks! Caroline Bass Assistant Professor University at Buffalo From edmartin26 at gmail.com Sun Jun 4 17:48:26 2017 From: edmartin26 at gmail.com (Eddie Martin) Date: Sun, 4 Jun 2017 18:48:26 -0400 Subject: [Histonet] What happened to Ultraclone/PGP9.5? Message-ID: Novocastra has been selling pgp 9.5 for years. I've used it on frozen sections and FFPR on 3 microns as well as 50 micron sections and it stains very well. Sent from my iPhone > On Jun 3, 2017, at 3:22 PM, Hobbs, Carl wrote: > > > > Ultraclone > THE supplier of PGP9.5 for many years ! > Respect to them/him/her/LTB > No longer....sigh. > Ultraclone: "legendary"...chuckle > > > Sure....other Suppliers now offer anti PGP9.5 abs > Why then do they still call it "PGP9.5"?? > We now know what the protein is. > > PGP: "Pretty good protein" I recall? > Why the 9.5? > Usually a clone but, but Ultraclone ab was a rabbit poly. > > One could only order via fax, I recall. > Isle of Wight location: Rossiters Farm. > > Any more info re Ultraclone's history would be most appreciated. > > Curiously > > Carl > > > From Nilufa.Sultana at uts.edu.au Sun Jun 4 18:10:52 2017 From: Nilufa.Sultana at uts.edu.au (Nilufa Sultana) Date: Sun, 4 Jun 2017 23:10:52 +0000 Subject: [Histonet] About Histology processor Message-ID: <4ef32d60f8e043c8a82cd9e8e6ffa7ad@MBS04.adsroot.uts.edu.au> Hi Everyone, I am Nilufa from Histology and Anatomy department, University of technology, want to know about the Tissue Processor. For our lab we want to replace the old Thermo Fisher Shandon Excelsior ( A78410100) with Leica HistoCore Pearl ( 200 cassettes) or new model of Thermo Scientific Excelsior As Tissue Processor (200 cassettes). We are processing tissue for teaching/research purpose. It will be appreciated if I can know which one is better and any user have any issues with those models of tissue processor. Thanks With regards Nilufa Histology and Anatomy department University of Technology UTS CRICOS Provider Code: 00099F DISCLAIMER: This email message and any accompanying attachments may contain confidential information. If you are not the intended recipient, do not read, use, disseminate, distribute or copy this message or attachments. If you have received this message in error, please notify the sender immediately and delete this message. Any views expressed in this message are those of the individual sender, except where the sender expressly, and with authority, states them to be the views of the University of Technology Sydney. Before opening any attachments, please check them for viruses and defects. Think. Green. Do. Please consider the environment before printing this email. From tony.henwood at health.nsw.gov.au Sun Jun 4 19:23:51 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon, 5 Jun 2017 00:23:51 +0000 Subject: [Histonet] Method for Patzelt stain Message-ID: <0237449DE79DBC45B686AB82CDCD16FF95558025@SVDCMBX-MEX008.nswhealth.net> Hi all, I am having difficulty locating the method for the Patzelt stain. I came across it while reading: Kimura, S., Hirai, A., & Shimizu, H. (1981). Epidermal vacuolation: an artifact due to injection of local anesthetics. Archives of dermatological research, 270(4), 413-419. This paper references a German publication entitled: Patzelt V (1926) Zum Bau der menschlichen Epidermis. Z Mikroskop Anat Forsch 5:371- 462 Would anyone out there have an English translation of this method? One of my Grad students is interested. Thanks muchly Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From mills at 3scan.com Mon Jun 5 09:40:50 2017 From: mills at 3scan.com (Caroline Miller) Date: Mon, 5 Jun 2017 07:40:50 -0700 Subject: [Histonet] What happened to Ultraclone/PGP9.5? In-Reply-To: References: Message-ID: I have had very good luck with the Dako pgp9.5 in both slides, as well as whole mount staining on skin. mills On Sun, Jun 4, 2017 at 3:48 PM, Eddie Martin via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Novocastra has been selling pgp 9.5 for years. I've used it on frozen > sections and FFPR on 3 microns as well as 50 micron sections and it stains > very well. > > Sent from my iPhone > > > On Jun 3, 2017, at 3:22 PM, Hobbs, Carl wrote: > > > > > > > > Ultraclone > > THE supplier of PGP9.5 for many years ! > > Respect to them/him/her/LTB > > No longer....sigh. > > Ultraclone: "legendary"...chuckle > > > > > > Sure....other Suppliers now offer anti PGP9.5 abs > > Why then do they still call it "PGP9.5"?? > > We now know what the protein is. > > > > PGP: "Pretty good protein" I recall? > > Why the 9.5? > > Usually a clone but, but Ultraclone ab was a rabbit poly. > > > > One could only order via fax, I recall. > > Isle of Wight location: Rossiters Farm. > > > > Any more info re Ultraclone's history would be most appreciated. > > > > Curiously > > > > Carl > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From amurvosh at advancederm.net Mon Jun 5 13:34:21 2017 From: amurvosh at advancederm.net (Anne Murvosh) Date: Mon, 5 Jun 2017 18:34:21 +0000 Subject: [Histonet] CEU's Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7B52779@Exchange.Advancederm.net> I was wondering if college classed not related to histology counted for continuing education. I'm going back to school and although I think science classes would count, I wasn't sure about a math or English class. Does anyone know for sure. Thanks Anne From rsrichmond at gmail.com Mon Jun 5 15:55:32 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Mon, 5 Jun 2017 16:55:32 -0400 Subject: [Histonet] Method for Patzelt stain Message-ID: Tony Henwood is trying to obtain an English translation for a "Patzelt stain": >>This paper references a German publication entitled: Patzelt V (1926) Zum Bau der menschlichen Epidermis. Z Mikroskop Anat Forsch 5:371- 462. Would anyone out there have an English translation of this method?<< If you can get a copy of the German publication, I can look through it for the stain method and translate it for you. Bob Richmond Samurai Pathologist Maryville, Tennessee From isabelsoto1162 at yahoo.com Mon Jun 5 16:11:38 2017 From: isabelsoto1162 at yahoo.com (Isabel Soto) Date: Mon, 5 Jun 2017 21:11:38 +0000 (UTC) Subject: [Histonet] CEU's In-Reply-To: <22BDD9AABC13E24E95D1CF064B75C4B7B52779@Exchange.Advancederm.net> References: <22BDD9AABC13E24E95D1CF064B75C4B7B52779@Exchange.Advancederm.net> Message-ID: <1348646573.2809982.1496697098560@mail.yahoo.com> No they do not count. Sent from Yahoo Mail on Android On Mon, Jun 5, 2017 at 11:54 AM, Anne Murvosh via Histonet wrote: I was wondering if college classed not related to histology counted for continuing education. I'm going back to school and although I think science classes would count, I wasn't sure about? a math or English class. Does anyone know for sure. Thanks Anne _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at dpspa.com Tue Jun 6 11:48:56 2017 From: criley at dpspa.com (Charles Riley) Date: Tue, 6 Jun 2017 12:48:56 -0400 Subject: [Histonet] Tissue processing schedules Message-ID: My lab just recently purchased a Tissue-Tek VIP 6 AI processor and I was wondering if anyone would be willing to share their tissue processing protocols. We process small G i biopsies as well as large skins and breast specimens. Trying to get a good idea about where to start my processing testing. Thanks -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From angie at ka-recruiting.com Tue Jun 6 12:09:01 2017 From: angie at ka-recruiting.com (Angie Laparidis) Date: Tue, 6 Jun 2017 13:09:01 -0400 Subject: [Histonet] Portland, OR Histology Openings Message-ID: Hi Histonet Users! I wanted to update you and let you know that my clients in the *Portland, OR* area are looking for a Full-time *Histotech *and a *Histology Supervisor* to join their teams! Let me know if this is an area of interest and *please send over an updated resume*. Thanks! Sincerely, *Angie Laparidis*Healthcare Recruiter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South, Boston, MA, 02109 W: 617.746-2744 (*please note this is a new number*) F: (617) 507-8009 Angie at ka-recruiting.com Our openings are updated daily at www.ka-recruiting.com From mburns at atlanticurologyclinics.com Tue Jun 6 12:12:44 2017 From: mburns at atlanticurologyclinics.com (Melissa Burns) Date: Tue, 6 Jun 2017 17:12:44 +0000 Subject: [Histonet] Desperatly Seeking Coredishes Message-ID: <8C537C608EA0194B87F6DE7BC6E1BF885075DA40@AUC-Exchange2.gsuro.com> Does anyone know how we can get our hands on some prostate biopsy (12 part) coredishes from Simport???? All of my vendors are saying NONE available until the 15th or later from distributors.... Anyone?!?!?!? Melissa Burns, HT Pathology Lab Manager Atlantic Urology Clinics Pathology Lab 3600 Sea Mountain Hwy. Suite D Little River, SC 29566 843-399-8930 phone 843-399-8932 fax From relia1 at earthlink.net Tue Jun 6 13:19:25 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 6 Jun 2017 14:19:25 -0400 Subject: [Histonet] The Catbird Seat in 2017 - What's New? Message-ID: <193001d2def1$6de08140$49a183c0$@earthlink.net> Hi Histonetters, How are you? I hope you are having a great week! Last year I sent you an email telling you that about the old saying ?In the Catbird Seat? It means you are calling the shots. That?s what was starting to happen last year in the histology field and it?s gotten even BETTER since then. ? If you are an ASCP certified histotech with at least 2 years of experience ? If you are a recent graduate of a histology program who is ASCP certified or eligible ? If you are an experienced tech with more than 2 years of experience not certified but eligible and willing to get certified EVERY ONE OF YOU is in the CATBIRD SEAT!!!!! Things have come full cycle and you are in GREAT DEMAND. There are many more histology jobs than histotechs. There are sign on bonuses, leading edge training opportunities and amazing perks waiting for you!! If you are considering a job change: ? Because you want to relocate ? Because you feel unchallenged ? Because you want more money, a better shift, nicer benefits Whatever the reason **STRIKE WHILE THE IRON?S HOT!! Shoot me a quick email and let me know what you would like to do and where you would like to go and when (no pressure the timing is up to YOU!) I will keep you posted on opportunities that match your interests. **REMEMBER IT NEVER HURTS TO LOOK!! I am including a list of my current opportunities in case one might strike your fancy. Please feel free to pass the info along to your friends and coworkers. If I place someone you refer to me you will earn a referral reward! RELIA?S Current Histology Opportunities Los Angeles, CA Histology Lab Manager Birmingham, AL Histology Supervisor Modesto, CA IHC Specialist Modesto, CA Histology Tech San Diego, CA Histology Tech Chicago, IL Clinical histotech Chicago, IL Research histotech Tyler, TX Dermpath Histotech Glenwood Springs, CO Histotechnician Cranford, NJ Histology Tech Birmingham, AL Dermpath Histotech Nashville, TN Histotechnician All of my clients offer excellent compensation, benefits and some offer relocation assistance and or sign on bonuses. All of these jobs are full time & permanent & most of them are RELIA Exclusives!!! I can be reached ASAP via email at relia1 at earthlink.net or toll free at the office at 866-607-3542 or on my cell at 407-353-5070 call or text! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Jeff.Howery at kadlecmed.org Tue Jun 6 17:26:04 2017 From: Jeff.Howery at kadlecmed.org (Howery, Jeff L) Date: Tue, 6 Jun 2017 15:26:04 -0700 Subject: [Histonet] specimen log in sheets Message-ID: We have had some controversy regarding the use of specimen log sheets. Does your Institution require, to have the specimens logged in along with the Patients name? Ours, currently require the Patient Information Sticker, Department #of containers, Time, Routine Fresh Frozen, Staff Initials, and Specimen Description. The Specimen Description is what this person wants to get rid of. Any input would be greatly appreciated. Also, if you can give me the size of your Institution Please. Regards, Jeff From INAIR at coh.org Tue Jun 6 19:20:20 2017 From: INAIR at coh.org (Nair, Indu) Date: Wed, 7 Jun 2017 00:20:20 +0000 Subject: [Histonet] IHC Message-ID: Hello Histoneters, I am relatively new to IHC although I have several years experience in IF I am trying to set up IHC and one of the problems I face is non-differentiation between nuclear and HRP staining. Any suggestions or advice? Thankyou Indu --------------------------------------------------------------------- -SECURITY/CONFIDENTIALITY WARNING- This message (and any attachments) are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (LCP301) --------------------------------------------------------------------- From Richard.Cartun at hhchealth.org Tue Jun 6 19:41:49 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Wed, 7 Jun 2017 00:41:49 +0000 Subject: [Histonet] Question Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E954419BF@HHCEXCHMB03.hhcsystem.org> We are thinking of changing the way we report consult testing (IHC, Flow Cytometry, and Molecular) for our system hospitals. Instead of accessioning the consult case here, the requesting hospital would create a "Procedure/Addendum" in Sunquest CoPath for their specimen and then send us the unstained slides or paraffin block for testing. Once the testing is completed, the pathologist here (assigned to the case) would go into the other hospital's report, populate the "Procedure/Addendum" with the testing performed and the interpretation, and then sign it out. We would not have a consult accession number (or report) here at our hospital for this specimen. Does this make sense? Is anyone else in a multiple hospital system already doing this? I welcome all comments. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From LRaff at uropartners.com Wed Jun 7 06:28:05 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 7 Jun 2017 11:28:05 +0000 Subject: [Histonet] On-Call Histologist for Chicago Area Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF1144870D@COLOEXCH01.uropartners.local> We are seeking an on-call histologist at our suburban Chicago private lab to supplement our great current team of 4 histologists. Primarily late afternoon or evening hours to give us a hand cutting on those extra busy days! Please contact Andrea O'Brien, Histology Manager at 708-486-0076. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From john.frazier at roche.com Wed Jun 7 12:33:31 2017 From: john.frazier at roche.com (Frazier, John) Date: Wed, 7 Jun 2017 13:33:31 -0400 Subject: [Histonet] specimen log in sheets Message-ID: <-6335676496214575665@unknownmsgid> That's one of the most important aspects of the identity of the specimen. I would never consider dropping that. If anything I would consider dropping the patients name if the sign in sheet is in a public area due to HIPPA compliance Sent from my iPhone > On Jun 6, 2017, at 6:26 PM, Howery, Jeff L wrote: > > We have had some controversy regarding the use of specimen log sheets. Does your Institution require, to have the specimens logged in along with the Patients name? Ours, currently require the Patient Information Sticker, Department #of containers, Time, Routine Fresh Frozen, Staff Initials, and Specimen Description. The Specimen Description is what this person wants to get rid of. Any input would be greatly appreciated. Also, if you can give me the size of your Institution Please. > > Regards, > Jeff > From LRaff at uropartners.com Wed Jun 7 12:54:34 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 7 Jun 2017 17:54:34 +0000 Subject: [Histonet] PRN-oncall histology openning in Chicago Area. Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF11449C5F@COLOEXCH01.uropartners.local> We are seeking an on-call histologist at our suburban Chicago private lab to supplement our great current team of 4 histologists. Primarily late afternoon or evening hours to give us a hand cutting on those extra busy days! Please contact Andrea O'Brien, Histology Manager at 708-486-0076. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From jvickroy at SpringfieldClinic.com Wed Jun 7 13:14:03 2017 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Wed, 7 Jun 2017 18:14:03 +0000 Subject: [Histonet] Balance calibration Message-ID: <9B1A1501A800064397369BD8072E6BCA6D4C3892@E2K10DB.springfieldclinic.com> We use two very basic electronic balances in the histology lab at the clinic. There is very limited use of these instruments. One is we weigh an amount of diastase when performing a PAS stain with diastase and we weigh the bladder and prostate TUR specimens. Is it necessary to have some sort of calibration system or annual review for CAP? While I was in the hospital we used balances for measuring IHC reagents and of course we needed very precise measurements however in the clinic we don't do any IHC stains and the special stains are limited to PAS and AFB stains. We hired a third party company to calibrate all of the balances throughout the entire lab at the hospital. The small lab at the clinic does not have any balances in usage besides the ones in Histology. Any thoughts? Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From mtoole at dcol.net Wed Jun 7 14:25:14 2017 From: mtoole at dcol.net (Mike Toole) Date: Wed, 7 Jun 2017 14:25:14 -0500 Subject: [Histonet] Balance calibration In-Reply-To: <9B1A1501A800064397369BD8072E6BCA6D4C3892@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA6D4C3892@E2K10DB.springfieldclinic.com> Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB351E69D8A50@mail> James, I'm not sure about the necessity of it, but it just seems to be good practice to verify that your equipment works as intended. Especially if the manufacturer states that verification checks should be performed. Check the user's manual or contact the manufacturer. Manufacturers often have a technical specialist on staff that can be helpful with those types of questions. Mike -----Original Message----- From: Vickroy, James via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, June 07, 2017 1:14 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] Balance calibration We use two very basic electronic balances in the histology lab at the clinic. There is very limited use of these instruments. One is we weigh an amount of diastase when performing a PAS stain with diastase and we weigh the bladder and prostate TUR specimens. Is it necessary to have some sort of calibration system or annual review for CAP? While I was in the hospital we used balances for measuring IHC reagents and of course we needed very precise measurements however in the clinic we don't do any IHC stains and the special stains are limited to PAS and AFB stains. We hired a third party company to calibrate all of the balances throughout the entire lab at the hospital. The small lab at the clinic does not have any balances in usage besides the ones in Histology. Any thoughts? Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From atempleton at tvmdl.tamu.edu Wed Jun 7 15:09:55 2017 From: atempleton at tvmdl.tamu.edu (Alexis Templeton) Date: Wed, 7 Jun 2017 20:09:55 +0000 Subject: [Histonet] Air Scrubber Message-ID: We've recently run into a little trouble with fumes in our lab. We gross all of our own tissue and the biggest problem is with formalin fumes in our grossing room. Does anyone have experience with air scrubbers that would help? Recommendations would be great. Bad experiences also welcome. Thanks! Alexis Templeton, HT (ASCP)CM Diagnostic Laboratory Supervisor Histopathology Texas A&M Veterinary Medical Diagnostic Laboratory P.O. Drawer 3040 | College Station, TX 77841-3040 p: (979) 845-3414 | f: (979) 845-1794 atempleton at tvmdl.tamu.edu http://tvmdl.tamu.edu We Moved! Effective February 27, 2017 our physical (shipping) address is 483 Agronomy Road, College Station, TX 77840. Our billing address remains at PO Drawer 3040, College Station, Texas 77841 ________ **The contents of this email do not necessarily represent the views or policies of TVMDL. This email is intended for the recipient only and the information should not be released to third parties. From hhuggins at novalabs.co Thu Jun 8 13:54:57 2017 From: hhuggins at novalabs.co (Haley Huggins) Date: Thu, 8 Jun 2017 11:54:57 -0700 Subject: [Histonet] Question regarding frozen control tissue Message-ID: I am trying to find a way to obtain small bowel/intestine as a fresh/frozen sample to be able to use it for a control with my staining for PGP9.5 free floating IHC stain. Does anyone know where a good place would be to obtain the needed control tissue? *Haley Huggins, HT (ASCP)cm* *Technical Lab Supervisor* *1050 Las Tablas Rd, * *Templeton, CA 93465* *Office: 877-230-1518* *Cell: 303-652-7453* From lmarie08 at uga.edu Thu Jun 8 14:58:34 2017 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Thu, 8 Jun 2017 19:58:34 +0000 Subject: [Histonet] plastic section gram stain Message-ID: Hello histonet world, My pathologist just gave me a slide with a section on it that is intended for electron microscopy. Has if anyone ever done a Brown and Hopps special on a section embedded in Epon/Araldite? She told me that her colleague has had the sections stained successfully with T-Blue on a hot plate before. But gram stains are a bit more complicated than a T-Blue! I can heat the staining components (i.e. crystal violet, safranin o, and iodine) but there are removal steps that require use of acetone, formaldehyde, acetic acid, and picric acid, and I am not sure how those reagents will work with the plastic. Any help or experience to share would be wonderful. Thanks, Lauren From SteveM at mcclainlab.com Thu Jun 8 15:28:24 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Thu, 8 Jun 2017 20:28:24 +0000 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: If it is any help, A dollar bill weighs 1 gram and a quarter weighs 5.67 grams. It is simpler to write up some calibration procedure based on knowns. Steve A. McClain, MD > On Jun 8, 2017, at 13:20, "histonet-request at lists.utsouthwestern.edu" wrote: > > Balance calibration > Message-ID: From Susan.Dachel at va.gov Fri Jun 9 09:55:16 2017 From: Susan.Dachel at va.gov (Dachel, Susan K.) Date: Fri, 9 Jun 2017 14:55:16 +0000 Subject: [Histonet] Histopathology Technologist Position Descriptions Message-ID: <0FFA32161E88AA42B1B7B0B19B43A542284A39F7@VAPNSMSGD53S01.vha.med.va.gov> I am representing VHA as a member of a Subject Matter Expert workgroup charged with developing VA Histopathology Technologist qualification standards. I am reaching out to ask if you will share your Histopathology Technologist position descriptions (or direct me to the appropriate person). I'd like both routine and specialized (Immunohistochemistry, Mohs, Molecular) position descriptions if you employ such occupations as well as manager/supervisory and/or lead position descriptions. The documents I am asking for are necessary in order for the group to conduct a job analysis from a representative number of position descriptions/job descriptions from both VHA facilities and external organizations. I appreciate any documents you can provide as well as information on education and certification requirements. Sue Dachel BA, HT(ASCP)CM Histology Supervisor Minneapolis VA Health Care System From rfisher0126 at msn.com Fri Jun 9 10:40:19 2017 From: rfisher0126 at msn.com (Renee Fisher) Date: Fri, 9 Jun 2017 15:40:19 +0000 Subject: [Histonet] Histo tech gross competency Message-ID: Hey Histo world, We have histo tech?s that do gross and we are in need of a good competency. Anyone out there have histo techs that gross and how do you do competency? Please share. Thank you Renee? From Richard.Cartun at hhchealth.org Fri Jun 9 12:34:14 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 9 Jun 2017 17:34:14 +0000 Subject: [Histonet] ClearRite Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E95442152@HHCEXCHMB03.hhcsystem.org> Anyone using ClearRite (xylene replacement) for tissue processing? If so, what's been your experience? Any effect on IHC or molecular testing? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From rsrichmond at gmail.com Sat Jun 10 13:32:46 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 10 Jun 2017 14:32:46 -0400 Subject: [Histonet] Clear-Rite 3 Message-ID: Richard W. Cartun, MS, PhD - Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory - Director, Biospecimen Collection Programs - Assistant Director, Anatomic Pathology, Hartford Hospital, Hartford, CT asks >>Anyone using ClearRite (xylene replacement) for tissue processing? If so, what's been your experience? Any effect on IHC or molecular testing?<< >From a procedure I wrote many years ago: "A widely used aliphatic substitute for xylene is Richard-Allan?s Clear-Rite 3?. They describe this product as a branched chain aliphatic blend of isoparaffinic oils, a mixture of synthetic isoparafinic [sic] aliphatic hydrocarbons, in the chemical family of synthetic aliphatic hydrocarbons (petroleum solvent). DOT calls the product petroleum naphtha. Its hazard label is 1 health hazard, 3 fire hazard, 0 reactivity, no specific hazard." This is a pretty inert material, and I wouldn't expect any special problems with it. "The Martin M. Berman MD" etc. - Hey, I was in residency with Marty Berman at Johns Hopkins around 1965. I knew he went off to Connecticut but I never heard of him again. Is he still working? Bob Richmond Samurai Pathologist Maryville TN On Sat, Jun 10, 2017 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. ClearRite (Cartun, Richard) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 9 Jun 2017 17:34:14 +0000 > From: "Cartun, Richard" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] ClearRite > Message-ID: > <9215BD4B0BA1B44D962A71C758B68D2E95442152 at HHCEXCHMB03. > hhcsystem.org> > Content-Type: text/plain; charset="us-ascii" > > Anyone using ClearRite (xylene replacement) for tissue processing? If so, > what's been your experience? Any effect on IHC or molecular testing? > Thank you! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & > Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 163, Issue 9 > **************************************** > From JMacDonald at mtsac.edu Sat Jun 10 16:02:45 2017 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Sat, 10 Jun 2017 14:02:45 -0700 Subject: [Histonet] ClearRite In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E95442152@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E95442152@HHCEXCHMB03.hhcsystem.org> Message-ID: For many years we used ClearRite, both on our tissue processor and for staining. We are now using a similar product from another company. We did not see any problems with our IHC with the use of this product. We do not do molecular testing. We found it to be far superior to the limonenes and much safer to use than xylene. Coverslipping is another issue. Not all mounting media are compatible with the aliphatic hydrocarbons. We have good success with Permount. Jennifer MacDonald From: "Cartun, Richard via Histonet" To: "histonet at lists.utsouthwestern.edu" Date: 06/09/2017 10:36 AM Subject: [Histonet] ClearRite Anyone using ClearRite (xylene replacement) for tissue processing? If so, what's been your experience? Any effect on IHC or molecular testing? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From esarricks at gmail.com Sat Jun 10 19:55:32 2017 From: esarricks at gmail.com (Erin Galati) Date: Sat, 10 Jun 2017 20:55:32 -0400 Subject: [Histonet] Sheep tissue Message-ID: <43FE66BF-146D-4C3F-9F0A-80F27B615EDE@gmail.com> Hi all, Hope you are having a great weekend! We are looking to optimize caspase-3 in sheep and need to get our hands on some spleen, lymph node, or small intestine tissues from a sheep. Either wet tissue or block form would be ok! Please let me know if you know of any place that could help me out. Thanks in advance for your help! Regards, Erin From michaelpathology at live.com Sun Jun 11 12:11:56 2017 From: michaelpathology at live.com (Michael Backhus) Date: Sun, 11 Jun 2017 17:11:56 +0000 Subject: [Histonet] Techniques of histotechnicians Message-ID: Would anybody like to share their histology techniques learned over the years? An example would be putting alcohol in the water bath to keep out wrinkles. Another could be as simple as blowing on a block while cutting with a microtome. I love learning new techniques. They can be common simple practices or uniques techniques that techs may not know. Thank you Mike HT ASCP Sent from my iPhone From mjdessoye at commonwealthhealth.net Mon Jun 12 06:41:36 2017 From: mjdessoye at commonwealthhealth.net (Dessoye, Michael) Date: Mon, 12 Jun 2017 11:41:36 +0000 Subject: [Histonet] ClearRite In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E95442152@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E95442152@HHCEXCHMB03.hhcsystem.org> Message-ID: We've used ClearRite 3 and had no issues with IHC or molecular. Michael J. Dessoye, M.S.?|?Histology/Toxicology/Special Chemistry Supervisor?|?Commonwealth Health Laboratory Services |?mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 -----Original Message----- From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] Sent: Friday, June 09, 2017 1:34 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] ClearRite Anyone using ClearRite (xylene replacement) for tissue processing? If so, what's been your experience? Any effect on IHC or molecular testing? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From TNMayer at mdanderson.org Mon Jun 12 12:23:31 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Mon, 12 Jun 2017 17:23:31 +0000 Subject: [Histonet] Techniques of histotechnicians Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8839F1D6E5@D1PWPEXMBX08.mdanderson.edu> One technique I use to cut uterus is to face the blocks at room temp, or even a little warmer. Then I place them on my ice tray and allow them to chill and soak up a lot of water. Then they are cut last in my set. This allows them to "cut like butter". My micrometer is set at 3, not 4, and I get nice thin, smooth sections. No chatter, or swiss cheese. It works really great when there are fibroids, because then knife goes through with less resistance. Toysha Mayer Message: 1 Date: Sun, 11 Jun 2017 17:11:56 +0000 From: Michael Backhus To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Techniques of histotechnicians Message-ID: Content-Type: text/plain; charset="us-ascii" Would anybody like to share their histology techniques learned over the years? An example would be putting alcohol in the water bath to keep out wrinkles. Another could be as simple as blowing on a block while cutting with a microtome. I love learning new techniques. They can be common simple practices or uniques techniques that techs may not know. Thank you Mike HT ASCP Sent from my iPhone The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From jaylundgren at gmail.com Mon Jun 12 15:26:10 2017 From: jaylundgren at gmail.com (Jay Lundgren) Date: Mon, 12 Jun 2017 15:26:10 -0500 Subject: [Histonet] ClearRite In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E95442152@HHCEXCHMB03.hhcsystem.org> Message-ID: I've worked in labs using both Clearite and d-limonene, and both are fine for IHC and molecular studies. Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Jun 12, 2017 at 6:41 AM, Dessoye, Michael via Histonet < histonet at lists.utsouthwestern.edu> wrote: > We've used ClearRite 3 and had no issues with IHC or molecular. > > Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry > Supervisor | Commonwealth Health Laboratory Services | mjdessoye@ > commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | > Tel: 570-552-1432 | Fax: 570-552-1484 > > > -----Original Message----- > From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] > Sent: Friday, June 09, 2017 1:34 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] ClearRite > > Anyone using ClearRite (xylene replacement) for tissue processing? If so, > what's been your experience? Any effect on IHC or molecular testing? > Thank you! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & > Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your computer. > Do not deliver, distribute or copy this message and do not disclose its > contents or take any action in reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From garethdavisyuma at gmail.com Mon Jun 12 17:29:52 2017 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Mon, 12 Jun 2017 15:29:52 -0700 Subject: [Histonet] Proficiency Testing for small labs Message-ID: I was wondering what other small CAP accredited GI labs did for proficiency testing. The lab I work in only does H&Es, and two IHC stains (H. pylori and CD8). that's it. All the PT programs with CAP are overkill for what little we do. In the past I have sent slides reflecting my work to an outside pathologist to review. He would send back a evaluation based on my work. But, this year my inspection is due and the letter informing me of the inspection stated that a CAP approved PT had to be used. If anyone has any first hand knowledge on what I should do, please feel free to email me. Thanks! -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From olsonlc at vcu.edu Tue Jun 13 09:24:59 2017 From: olsonlc at vcu.edu (Lucas Olson) Date: Tue, 13 Jun 2017 10:24:59 -0400 Subject: [Histonet] Eliminating air bubbles when mounting coverslip Message-ID: Dear all, Does anyone have good advice on preventing or eliminating bubbles when coverslipping? Thanks! Lucas Olson From rochelehaynes at yahoo.com Tue Jun 13 13:16:21 2017 From: rochelehaynes at yahoo.com (Rochele Haynes) Date: Tue, 13 Jun 2017 18:16:21 +0000 (UTC) Subject: [Histonet] Quality Assurance GLP Contract Job Opening References: <423832128.9076222.1497377781836.ref@mail.yahoo.com> Message-ID: <423832128.9076222.1497377781836@mail.yahoo.com> QAU Contract Position for GLP Lab to conduct in-phase audits for histology (grossing, embedding, microtoming) and clinical pathology (hematology and clinical chemistry) in Fort Collins, CO E-mail Rochele at rochelehaynes at yahoo.com or call (970) 691-6913 From teri.johnson at navigatebp.com Tue Jun 13 14:56:22 2017 From: teri.johnson at navigatebp.com (johnson, teri) Date: Tue, 13 Jun 2017 19:56:22 +0000 Subject: [Histonet] Proficiency Testing for small labs Message-ID: <4ea6428107934c5caf0c3cd55d73af20@BN1PR62MB038.023d.mgd.msft.net> Hi Gareth, Good luck on your upcoming inspection. Regarding Proficiency testing, labs that don't do the usual barrage of special or immunohistochemical stains need to have some sort of alternate plan for PT. Our lab has outlined alternative assessment in our Quality Assurance Program. It requires that you provide details in how you perform all your quality items for the lab, including the PT (number of samples, method of comparative testing, criteria for successful and unsuccessful results, investigation details, documentation process, etc.) See the information from www.cap.org on this: "Use alternative assessment methods, when necessary. Tests for which PT is not available or that do not require enrollment in a formal PT program must be assessed twice a year to confirm their reliability. Methods of checking accuracy include split sampling with another laboratory, the use of previously assayed specimens, pooled material, use of PT products usually educational in nature, or clinical correlation. Another example of alternative assessment is the CAP's Sample Exchange Registry, which helps facilitate cooperation around genetic testing. It is important to emphasize to everyone that just performing an alternative assessment is insufficient. As the laboratory medical director, your job is to establish evaluation criteria for the alternative assessment. If a result falls outside the established acceptable range, conduct an investigation in the same manner as you would with formal PT." In short, we have a bank of paraffin blocks that we use specifically for a specific assay's PT (previously qualified with known expression levels). Every 6 months we perform our particular IHC stain using samples that have been blinded to the operator and pathologist. The operator performs a routine run on those samples and submits them to the pathologist for scoring. Those scores are recorded on a specific document and compared against the previous 6 month score. Concordance must be established according to a pre-defined threshold. It passes or fails, and any failure must be investigated, explained, and re-tested if applicable. Do this for each of your IHC assays. Run more than one sample, and when possible do both positives and negatives ; we do either 3 (100% concordance required) or 5 (80% concordance required, but any failure is still investigated). Best wishes, Teri Johnson, HT(ASCP)QIHC Manager, Clinical Trial Testing T +1 760 516 5954 teri.johnson at navigatebp.com Navigate BioPharma, Inc. A Novartis Company 1890 Rutherford Rd. Carlsbad, CA 92008 USA From lynettepav at gmail.com Tue Jun 13 19:11:13 2017 From: lynettepav at gmail.com (Lynette Pavelich) Date: Tue, 13 Jun 2017 20:11:13 -0400 Subject: [Histonet] ClearRite In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E95442152@HHCEXCHMB03.hhcsystem.org> Message-ID: <13CDFA39-FC28-4160-A65D-0C3F9741A8D9@gmail.com> I would still recommend using real xylene on the processor cleaning cycle and any automated coverslipper you have. Lynette Pavelich, HT(ASCP) Premier Technical Consultant Sent from my iPad > On Jun 12, 2017, at 7:41 AM, Dessoye, Michael via Histonet wrote: > > We've used ClearRite 3 and had no issues with IHC or molecular. > > Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | Commonwealth Health Laboratory Services | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 > > > -----Original Message----- > From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] > Sent: Friday, June 09, 2017 1:34 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] ClearRite > > Anyone using ClearRite (xylene replacement) for tissue processing? If so, what's been your experience? Any effect on IHC or molecular testing? Thank you! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rhoobarb23 at comcast.net Wed Jun 14 06:54:11 2017 From: rhoobarb23 at comcast.net (RC) Date: Wed, 14 Jun 2017 06:54:11 -0500 Subject: [Histonet] Histologist In Oregon Message-ID: <898EAA1E-1085-403C-BFFB-041DD1C08FD4@comcast.net> Hey fellow histologists! Top of the morning. I?m looking for histology positions in the state of Oregon. 16 years of experience covering all facets of histology (R&D, Drug Discovery, Clinical Diagnostic, Reference) If any of your laboratories are in need, please contact me. Grazie! Rueben Carter From olsonlc at vcu.edu Wed Jun 14 08:15:15 2017 From: olsonlc at vcu.edu (Lucas Olson) Date: Wed, 14 Jun 2017 09:15:15 -0400 Subject: [Histonet] H + E Staining protocols Message-ID: Dear All, There are many variants to the standard H + E protocol. I'm seeking to optimize the current protocol our laboratory uses (pasted below). Do you guys have any suggestions of optimization (e.g. cutting down on the long water rinse after hematoxylin and increasing bluing reagent time)? Or if you have protocols that you'd think would be better please let me know. Our lab deals primarily with bone/cartilage/muscle. Our current protocol: Staining Preparation 1. After sectioning samples, dry in oven. Time Temp C Overnight 37 2-3 hours 45 30 minutes 55 Deparaffinization 1. Remove paraffin with xylene a. 2 minutes xylene b. 2 minutes in fresh xylene c. 2 minutes in fresh xylene d. 2 minutes in fresh xylene 2. Remove excess xylene 3. Rehydrate samples a. 1 minute absolute EtOH b. 1 minute different bucket of absolute EtOH c. 30 seconds in 95% absolute EtOH d. 45 seconds in 70% EtOH 4. Rinse with water for 1 minute Hematoxylin and Eosin Staining 1. Stain in hematoxylin (VWR 95057-858) for 5-10 minutes a. Optimized time for calvarium bone: 10 minutes b. Solution may be filtered to remove oxidized particles 2. Rinse in running tap water for 20 minutes 3. Decolorize in Clarifier 1 (1 second) a. Longer time (up to 3 seconds) will yield a lighter color. Discard after each use. 4. Immerse in VWR Bluing Reagent (30 sec- 1 minute) a. Dilute 25mL bluing reagent in 475 mL ultrapure H2O to working stock b. Optimized time for calvarium bone: 90 seconds 5. Rinse well in tap water (5 minutes) 6. Rinse in 95% ETOH (30 sec- 1 minute) 7. Counterstain in Eosin (30 sec- 90 sec) a. Optimized time for calvarium bone: 30 seconds 8. Dehydrate a. ETOH 95% (3 minutes) b. ETOH 95% (3 minutes) c. ETOH 100% (3 minutes) d. ETOH 100% (3 minutes) 9. Clear in Xylene (5 minutes) 10. Clear in new Xylene (5 minutes) 11. Mount with Cytoseal in fume hood and coverslip Thanks! Lucas Olson From olsonlc at vcu.edu Wed Jun 14 08:20:18 2017 From: olsonlc at vcu.edu (Lucas Olson) Date: Wed, 14 Jun 2017 09:20:18 -0400 Subject: [Histonet] Paraffin histology with Muscle Message-ID: Dear all, Does anyone have optimization tips for using paraffin histology with muscle? We do have access to a cryostat to do frozen histology, but it's not our lab's thus we have to pay to use it. We do have all the materials necessary for paraffin-based histology though. I've noticed that muscle can be more difficult to use with paraffin. Do you guys have any processing or cutting tips? Thanks! Lucas Olson From criley at dpspa.com Wed Jun 14 08:39:11 2017 From: criley at dpspa.com (Charles Riley) Date: Wed, 14 Jun 2017 09:39:11 -0400 Subject: [Histonet] Microwave processors Message-ID: Does anyone have any suggestions for microwave processing units? My lab processes about 110 blocks max a day from GI biopsies to breast tissue on size Sent from my iPhone From LRaff at uropartners.com Wed Jun 14 08:50:46 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 14 Jun 2017 13:50:46 +0000 Subject: [Histonet] Microwave processors In-Reply-To: References: Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF1146E8B3@COLOEXCH01.uropartners.local> We are quite happy with Milestone Histos3 for processing our prostate and bladder biopsies quickly. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, June 14, 2017 8:39 AM To: Histo List Subject: [Histonet] Microwave processors Does anyone have any suggestions for microwave processing units? My lab processes about 110 blocks max a day from GI biopsies to breast tissue on size Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton at cua.md Wed Jun 14 08:51:22 2017 From: wbenton at cua.md (Walter Benton) Date: Wed, 14 Jun 2017 13:51:22 +0000 Subject: [Histonet] Microwave processors In-Reply-To: References: Message-ID: <8defccecec204681ba8b0dab01175ab5@MAIL01.GCU-MD.local> Charles, Milestone Medical http://www.milestonemed.com/ The processors are great. Easy to operate and use minimal chemicals based on the protocols you select. H&E and IHC are very good. Check out the various product offerings to see which unit will meet your needs best. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, June 14, 2017 9:39 AM To: Histo List Subject: [Histonet] Microwave processors Does anyone have any suggestions for microwave processing units? My lab processes about 110 blocks max a day from GI biopsies to breast tissue on size Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From mlm11 at cornell.edu Wed Jun 14 09:17:24 2017 From: mlm11 at cornell.edu (Mary Lou Norman) Date: Wed, 14 Jun 2017 14:17:24 +0000 Subject: [Histonet] 3D plastic Message-ID: Hello Histonet, I've been asked to section 3D plastic with tissue attached. I will be finding out what type of plastic it is. Has anyone done this? Will I still be able to use paraffin? Thanks for any information. Mary Lou Norman NYS College of Veterinary Medicine Cornell University From Katie at PuyallupDerm.com Wed Jun 14 09:54:10 2017 From: Katie at PuyallupDerm.com (Katie) Date: Wed, 14 Jun 2017 07:54:10 -0700 Subject: [Histonet] Installing a new auto stainer Message-ID: <2D4B81BD3E41430E87C0F7A82A39A22A@LabHP> Hello, I am a small lab that has just relocated to a new building. As a part of the new lab construction we are installing our first auto-stainer. The plumber is concerned about any required permitting or precautions regarding back flow of reagents. I haven?t been able to find any information either way, because most labs have building operations management teams handle that end. Has anyone installed a new auto-stainer that knows if any special permitting, etc. is required? Thanks so much and feel free to contact me, Katie Riley Technical Supervisor of Dermatopathology Puyallup Dermatology Clinic katie at puyallupderm.com From isabelsoto1162 at yahoo.com Wed Jun 14 11:15:55 2017 From: isabelsoto1162 at yahoo.com (Isabel Soto) Date: Wed, 14 Jun 2017 16:15:55 +0000 (UTC) Subject: [Histonet] Histologist In Oregon In-Reply-To: <898EAA1E-1085-403C-BFFB-041DD1C08FD4@comcast.net> References: <898EAA1E-1085-403C-BFFB-041DD1C08FD4@comcast.net> Message-ID: <752676486.1577487.1497456955146@mail.yahoo.com> HI Rueben. There is 2 HT positions in Salem if you are interested. Calleven for details 407-307-8604? Sent from Yahoo Mail on Android On Wed, Jun 14, 2017 at 5:17 AM, RC via Histonet wrote: Hey fellow histologists! Top of the morning. I?m looking for histology positions in the state of Oregon. 16 years of experience covering all facets of histology (R&D, Drug Discovery, Clinical Diagnostic, Reference) If any of your laboratories are in need, please contact me. Grazie! Rueben Carter _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From djmr55 at hotmail.com Wed Jun 14 15:44:58 2017 From: djmr55 at hotmail.com (donna mihalik rossi) Date: Wed, 14 Jun 2017 20:44:58 +0000 Subject: [Histonet] Ventana Retic problems Message-ID: Hi Histonetters! We are experiencing sporadic staining of retic fibers from our Ventana Benchmark machines. We have 2 machines and are having the same problem on both. The machines were both decontaminated 2 weeks ago with all new solutions being made. The fibers are not crisp and are disconnected. Our control tissue is at the top of the slide with the patient tissue at the bottom. It appears that the control is staining better than the patient tissue but still is not crisp. We have tried different lots with the same result. Any comments before we consult Ventana? Your help would be appreciated. Thanks, Donna Rossi, PSU From JRobinson at pathology-associates.com Wed Jun 14 17:47:05 2017 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Wed, 14 Jun 2017 22:47:05 +0000 Subject: [Histonet] Ventana Retic problems In-Reply-To: References: Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829CB8538F08@PAEXCH1.PathologyAssociates.local> Hi Donna- I have been dealing with these same exact issues for 3 1/2 years now so I think I have achieved "expert status" in dealing with this problem. First, I'll give you my current protocol. When all things are working smoothly, it produces a nice stain. But it is very frustrating when it starts to "fade." We cut all retics at 5. Thinner cuts will definitely look lighter. Protocol: warmup slide: 47 degrees C. Oxidizer: 4 minutes Decolorizer: 4 minutes Sensitizer: 8 minutes Optimize Counterstain Intensity: 4 Minutes I have gone around and around with Ventana on decontamination problems with this instrument. I was performing full decontamination runs every month. My rep finally said to just decon the bulk wash carboy and the wash bottle on the instrument when fading begins to show. Ventana claims there will be a new wash out this year that will hopefully take care of the problem once and for all but no one at Ventana can give me a release date on that. In the meantime, this is what I do: Daily: run the "Purge Wash" function test 3 times in the morning before running any slides. Be sure to run the "Purge" function and not the "Prime" function. When loading slides, put all of your retic slides on after everything else (after the GMS, etc., slides). When staining starts to fade: after ruling out "thin cuts" and other obvious problems it is time for decon. The fading will show first on the patient tissue (the control may still look OK). Here is my current decon protocol: I use the Lysol IC decon solution. I have an extra 6 gallon carboy and I leave some made up (diluted) so that it is ready to go. Put some decon solution on a 4X4 guaze and wipe the dispenser tip underneath the top (lift lid up for access). Dump the wash solution in the wash bottle on the instrument. Rinse out with DI water. Fill with decon solution. Swish solution inside bottle. Replace bottle (on instrument). Run "Purge Wash" function test (3 times). After Purge #3, let the solution sit in the lines for at least 15 minutes (the longer the better). When you are ready to proceed, rinse bulk bottle well several times and replace with DI water. Run "Purge Wash" 3 more times. Time is not a factor here so you can just run them one after another. After the third purge, replace the DI with BM SS wash. Run "Purge Wash" function test 3 more times. That's it. I find I need to run this procedure about once a month. Additionally, I decon the 5 gallon bulk wash carboy EVERY TIME I make up a new batch. It doesn't take long (I just let the decon solution sit for 15 minutes) and then when I do need to do the modified decon on the instrument I do not need to worry about the bulk carboy. I hope this helps- it takes a little time but it does seems to help with the retic stain intensity. Jeff Robinson, Senior Histotechnologist (HT, HTL), Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: donna mihalik rossi via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, June 14, 2017 1:45 PM To: histonet Subject: [Histonet] Ventana Retic problems Hi Histonetters! We are experiencing sporadic staining of retic fibers from our Ventana Benchmark machines. We have 2 machines and are having the same problem on both. The machines were both decontaminated 2 weeks ago with all new solutions being made. The fibers are not crisp and are disconnected. Our control tissue is at the top of the slide with the patient tissue at the bottom. It appears that the control is staining better than the patient tissue but still is not crisp. We have tried different lots with the same result. Any comments before we consult Ventana? Your help would be appreciated. Thanks, Donna Rossi, PSU _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From cls71877 at gmail.com Wed Jun 14 18:15:33 2017 From: cls71877 at gmail.com (Cristi Rigazio) Date: Wed, 14 Jun 2017 19:15:33 -0400 Subject: [Histonet] Fixation and IHC Message-ID: Hi histonetters! Can anyone tell me what, if any, guidelines there are for fixation time for animal tissue with potential subsequent IHC stains. I am very familiar with CAP guidelines for receptor testing with breast cases, but I can't seem to find much on IHC for animal tissue. Thanks, Cristi From c.tague at Pathologyarts.com Wed Jun 14 19:03:00 2017 From: c.tague at Pathologyarts.com (Curt) Date: Thu, 15 Jun 2017 00:03:00 +0000 Subject: [Histonet] Ventana Retic problems In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829CB8538F08@PAEXCH1.PathologyAssociates.local> References: , <204A03EB5A7F0A4BB1EEDD52A963829CB8538F08@PAEXCH1.PathologyAssociates.local> Message-ID: <9C8F910F72893643B3C3793C3D67132B6811DD7A@PATHOLOGYSERVER.pathologyarts.local> We have had the same problem.... our solution was not the second but the wash, it for a bad too quickly so we don't make a full carboy and let it sit for a week, we make fresh wash almost daily, ESPECIALLY when we have a retic. Our stains look great daily with fresh wash solution. You may try that, hope it helps. Curt Sent from my Verizon, Samsung Galaxy smartphone -------- Original message -------- From: Jeffrey Robinson via Histonet Date: 6/14/17 4:01 PM (GMT-08:00) To: donna mihalik rossi Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana Retic problems Hi Donna- I have been dealing with these same exact issues for 3 1/2 years now so I think I have achieved "expert status" in dealing with this problem. First, I'll give you my current protocol. When all things are working smoothly, it produces a nice stain. But it is very frustrating when it starts to "fade." We cut all retics at 5. Thinner cuts will definitely look lighter. Protocol: warmup slide: 47 degrees C. Oxidizer: 4 minutes Decolorizer: 4 minutes Sensitizer: 8 minutes Optimize Counterstain Intensity: 4 Minutes I have gone around and around with Ventana on decontamination problems with this instrument. I was performing full decontamination runs every month. My rep finally said to just decon the bulk wash carboy and the wash bottle on the instrument when fading begins to show. Ventana claims there will be a new wash out this year that will hopefully take care of the problem once and for all but no one at Ventana can give me a release date on that. In the meantime, this is what I do: Daily: run the "Purge Wash" function test 3 times in the morning before running any slides. Be sure to run the "Purge" function and not the "Prime" function. When loading slides, put all of your retic slides on after everything else (after the GMS, etc., slides). When staining starts to fade: after ruling out "thin cuts" and other obvious problems it is time for decon. The fading will show first on the patient tissue (the control may still look OK). Here is my current decon protocol: I use the Lysol IC decon solution. I have an extra 6 gallon carboy and I leave some made up (diluted) so that it is ready to go. Put some decon solution on a 4X4 guaze and wipe the dispenser tip underneath the top (lift lid up for access). Dump the wash solution in the wash bottle on the instrument. Rinse out with DI water. Fill with decon solution. Swish solution inside bottle. Replace bottle (on instrument). Run "Purge Wash" function test (3 times). After Purge #3, let the solution sit in the lines for at least 15 minutes (the longer the better). When you are ready to proceed, rinse bulk bottle well several times and replace with DI water. Run "Purge Wash" 3 more times. Time is not a factor here so you can just run them one after another. After the third purge, replace the DI with BM SS wash. Run "Purge Wash" function test 3 more times. That's it. I find I need to run this procedure about once a month. Additionally, I decon the 5 gallon bulk wash carboy EVERY TIME I make up a new batch. It doesn't take long (I just let the decon solution sit for 15 minutes) and then when I do need to do the modified decon on the instrument I do not need to worry about the bulk carboy. I hope this helps- it takes a little time but it does seems to help with the retic stain intensity. Jeff Robinson, Senior Histotechnologist (HT, HTL), Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: donna mihalik rossi via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, June 14, 2017 1:45 PM To: histonet Subject: [Histonet] Ventana Retic problems Hi Histonetters! We are experiencing sporadic staining of retic fibers from our Ventana Benchmark machines. We have 2 machines and are having the same problem on both. The machines were both decontaminated 2 weeks ago with all new solutions being made. The fibers are not crisp and are disconnected. Our control tissue is at the top of the slide with the patient tissue at the bottom. It appears that the control is staining better than the patient tissue but still is not crisp. We have tried different lots with the same result. Any comments before we consult Ventana? Your help would be appreciated. Thanks, Donna Rossi, PSU _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. From edmartin26 at gmail.com Thu Jun 15 16:05:54 2017 From: edmartin26 at gmail.com (Eddie Martin) Date: Thu, 15 Jun 2017 17:05:54 -0400 Subject: [Histonet] Response Fixation and IHC for animal tissue Message-ID: <9B783AFB-E636-492A-AF82-CE49BF60505A@gmail.com> Hi, CAP guidelines don't cover animal testing or research testing. V/r, Eddie Martin HT, HTL, QIHC Sent from my iPhone > On Jun 14, 2017, at 7:15 PM, Cristi Rigazio wrote: > > Hi histonetters! > > Can anyone tell me what, if any, guidelines there are for fixation time for > animal tissue with potential subsequent IHC stains. I am very familiar > with CAP guidelines for receptor testing with breast cases, but I can't > seem to find much on IHC for animal tissue. > > Thanks, > Cristi > From cforster at umn.edu Thu Jun 15 16:19:53 2017 From: cforster at umn.edu (Colleen Forster) Date: Thu, 15 Jun 2017 16:19:53 -0500 Subject: [Histonet] Response Fixation and IHC for animal tissue In-Reply-To: <9B783AFB-E636-492A-AF82-CE49BF60505A@gmail.com> References: <9B783AFB-E636-492A-AF82-CE49BF60505A@gmail.com> Message-ID: Cristi, I follow the general guidelines of good fixation for the msajority. IF I am testing something that has specific in the clinicla lab, I try it the same on animal tissues for the same thing. Colleen Forster HT(ASCP)QIHC On Thu, Jun 15, 2017 at 4:05 PM, Eddie Martin via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi, > > CAP guidelines don't cover animal testing or research testing. > > V/r, > > Eddie Martin HT, HTL, QIHC > > Sent from my iPhone > > > On Jun 14, 2017, at 7:15 PM, Cristi Rigazio wrote: > > > > Hi histonetters! > > > > Can anyone tell me what, if any, guidelines there are for fixation time > for > > animal tissue with potential subsequent IHC stains. I am very familiar > > with CAP guidelines for receptor testing with breast cases, but I can't > > seem to find much on IHC for animal tissue. > > > > Thanks, > > Cristi > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From NMargaryan at luriechildrens.org Fri Jun 16 14:36:50 2017 From: NMargaryan at luriechildrens.org (Margaryan, Naira) Date: Fri, 16 Jun 2017 19:36:50 +0000 Subject: [Histonet] PD-L1 22c3 for IHC (paraffin) Message-ID: <34B2EDA118548A4EB35D6E650345BA6469C89862@SV-EX08.childrensmemorial.org> Dear Friends, Can any of you share with me a full detailed protocol (time and dilutions) after deparaffinization for the PD-L1 22c3 for IHC (paraffin) to use on DAKO (not Link 48) autostainer? http://www.agilent.com/en-us/products/pharmdx/pd-l1-ihc-22c3-pharmdx/pd-l1-ihc-22c3-pharmdx-for-autostainer-link-48-sk00621-5 Thanks in advance and have a nice weekend, Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) From jr at infosight.com Sat Jun 17 12:18:38 2017 From: jr at infosight.com (John Robertson) Date: Sat, 17 Jun 2017 13:18:38 -0400 Subject: [Histonet] Histonet Digest, Vol 163, Issue 16 In-Reply-To: References: Message-ID: Please cancel my subscription > On Jun 17, 2017, at 1:00 PM, histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. PD-L1 22c3 for IHC (paraffin) (Margaryan, Naira) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 16 Jun 2017 19:36:50 +0000 > From: "Margaryan, Naira" > To: "histonet-bounces at lists.utsouthwestern.edu" > , histonet-request > > Subject: [Histonet] PD-L1 22c3 for IHC (paraffin) > Message-ID: > <34B2EDA118548A4EB35D6E650345BA6469C89862 at SV-EX08.childrensmemorial.org> > > Content-Type: text/plain; charset="utf-8" > > Dear Friends, > > Can any of you share with me a full detailed protocol (time and dilutions) after deparaffinization for the PD-L1 22c3 for IHC (paraffin) to use on DAKO (not Link 48) autostainer? > > http://www.agilent.com/en-us/products/pharmdx/pd-l1-ihc-22c3-pharmdx/pd-l1-ihc-22c3-pharmdx-for-autostainer-link-48-sk00621-5 > > Thanks in advance and have a nice weekend, > Naira > > > Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) > > > This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 163, Issue 16 > ***************************************** From SteveM at mcclainlab.com Sat Jun 17 14:19:31 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Sat, 17 Jun 2017 19:19:31 +0000 Subject: [Histonet] Histonet Digest, Vol 163, Issue 15 Research histology and animal IHC In-Reply-To: References: Message-ID: <2469AC6F-978C-4287-8362-C10F1D0F0282@mcclainlab.com> Underfixation being the greater problem, for animal research I tell everyone 24 hours fixation. After 24 hours in clean formalin, Stop fixation by pouring off and transfer to 50% reagent alcohol. Using standard fixation and slow processing, repeatable near-perfect histology and IHC can be made w comparable staining in small biopsy and large samples alike. There is no right answer but kindly choose one method and teach that in your lab, better consistent than correct. IHC is antibody dependent and species dependent, and fixation dependent but IHC is especially section dependent. Quite predictably excellent slides and excellent IHC can be made from tissues in almost every species given 24 hours fixation and overnight processing, the methods we first learned in school 30-40 years ago. For 2mm samples in human clinical testing using common antibodies, we can acceptably shorten this to minimum 8-12 hours fixation and rapid 2 hour processing using fresh reagents w a wee bit of heat. But in research what is gained by it? Underfixed tissue causes more spurious results than over fixation. In weird species, having weird collagens weird cells, weird antibodies and weird questions all confounding the microscopist who needs spurious results? In research studies w IHC endpoints and morphometric work, we found results improved by stopping fixation at 24 hours, transferring the whole batch to 50% alcohol and then slow 12H to 20H processing wo heat. For really large slices processed in teabags or even toenails, an extended 16-20H processing can be useful. Adding more time in paraffin and xylene to the 12 hour. Counterintuitive in the era of rapid processing schedules, but slow processing produces consistent IHC results between small biopsy and large samples. Research processing for science RT 12 hours fresh reagents NO heat. NO formalin. We run ALL tissue processors wo formalin (station 1 is 50% alcohol). Long processing wo heat or prolonged 50% alcohol storage for days or weeks at RT does not appreciably harden tissue. Removing formalin completely from the tissue processors was done gradually, first on the animal research machines and rapid processing machines. Eventually the last VIP5 w the overnight run is where I met w resistance from my histotechs. Even though we had shown no issues w research samples or when short processing human samples, they wanted formalin on the processor- in case. Quite logically once fixed tissues are in 50% alcohol additional processing time in formalin serves no purpose. My histotechs belatedly gave in after we ran the processors for 6 months with only 1 minute in the formalin station 1. Our medical director covertly reprogrammed formalin station 1 to one minute and added back time to the alcohol in station 2 wo their knowledge (Choose your battles.) Steve A. McClain, MD McClain Laboratories, LLC On Jun 16, 2017, at 13:20, "histonet-request at lists.utsouthwestern.edu" wrote: >> Can anyone tell me what, if any, guidelines there are for fixation time for >> animal tissue with potential subsequent IHC stains. I am very familiar >> with CAP guidelines for receptor testing with breast cases, but I can't >> seem to find much on IHC for animal tissue. >> >> Thanks, >> Cristi From criley at dpspa.com Tue Jun 20 08:55:20 2017 From: criley at dpspa.com (Charles Riley) Date: Tue, 20 Jun 2017 09:55:20 -0400 Subject: [Histonet] Buying parts for machines Message-ID: Does anyone know where to purchase replacement sensors for a thermo shandon excelsior ES tissue processor? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From INAIR at coh.org Tue Jun 20 17:08:59 2017 From: INAIR at coh.org (Nair, Indu) Date: Tue, 20 Jun 2017 22:08:59 +0000 Subject: [Histonet] frozen sections In-Reply-To: References: Message-ID: Hello Histoneters! question: Does frozen section need Antigen Retrieval? thank you, for the anticipated timely help! indu --------------------------------------------------------------------- -SECURITY/CONFIDENTIALITY WARNING- This message (and any attachments) are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (LCP301) --------------------------------------------------------------------- From isabelsoto1162 at yahoo.com Tue Jun 20 18:29:19 2017 From: isabelsoto1162 at yahoo.com (Isabel Soto) Date: Tue, 20 Jun 2017 23:29:19 +0000 (UTC) Subject: [Histonet] frozen sections In-Reply-To: References: Message-ID: <1258283742.2752997.1498001360002@mail.yahoo.com> Yes.? Sent from Yahoo Mail on Android On Tue, Jun 20, 2017 at 3:15 PM, Nair, Indu via Histonet wrote: Hello Histoneters! question: Does frozen section need Antigen Retrieval? thank you, for the anticipated timely help! indu --------------------------------------------------------------------- -SECURITY/CONFIDENTIALITY WARNING- This message (and any attachments) are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (LCP301) --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw at yahoo.com Tue Jun 20 19:30:35 2017 From: slappycraw at yahoo.com (Larry Woody) Date: Wed, 21 Jun 2017 00:30:35 +0000 (UTC) Subject: [Histonet] frozen sections In-Reply-To: <1258283742.2752997.1498001360002@mail.yahoo.com> References: <1258283742.2752997.1498001360002@mail.yahoo.com> Message-ID: <2005028927.2802355.1498005035333@mail.yahoo.com> Tissue that does not go through formalin processing does not need AR. Sent from Yahoo Mail on Android On Tue, Jun 20, 2017 at 4:52 PM, Isabel Soto via Histonet wrote: Yes.? Sent from Yahoo Mail on Android ? On Tue, Jun 20, 2017 at 3:15 PM, Nair, Indu via Histonet wrote:? Hello Histoneters! question: Does frozen section need Antigen Retrieval? thank you, for the anticipated timely help! indu --------------------------------------------------------------------- -SECURITY/CONFIDENTIALITY WARNING- This message (and any attachments) are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (LCP301) --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw at yahoo.com Tue Jun 20 19:30:35 2017 From: slappycraw at yahoo.com (Larry Woody) Date: Wed, 21 Jun 2017 00:30:35 +0000 (UTC) Subject: [Histonet] frozen sections In-Reply-To: <1258283742.2752997.1498001360002@mail.yahoo.com> References: <1258283742.2752997.1498001360002@mail.yahoo.com> Message-ID: <2005028927.2802355.1498005035333@mail.yahoo.com> Tissue that does not go through formalin processing does not need AR. Sent from Yahoo Mail on Android On Tue, Jun 20, 2017 at 4:52 PM, Isabel Soto via Histonet wrote: Yes.? Sent from Yahoo Mail on Android ? On Tue, Jun 20, 2017 at 3:15 PM, Nair, Indu via Histonet wrote:? Hello Histoneters! question: Does frozen section need Antigen Retrieval? thank you, for the anticipated timely help! indu --------------------------------------------------------------------- -SECURITY/CONFIDENTIALITY WARNING- This message (and any attachments) are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (LCP301) --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From INAIR at coh.org Tue Jun 20 19:43:07 2017 From: INAIR at coh.org (Nair, Indu) Date: Wed, 21 Jun 2017 00:43:07 +0000 Subject: [Histonet] frozen sections In-Reply-To: <2005028927.2802355.1498005035333@mail.yahoo.com> References: <1258283742.2752997.1498001360002@mail.yahoo.com>, <2005028927.2802355.1498005035333@mail.yahoo.com> Message-ID: The tissue has been fixed in 4% PFA. Thank you! indu ________________________________________ From: Larry Woody via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 20, 2017 5:30 PM To: histonet at lists.utsouthwestern.edu; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] frozen sections Tissue that does not go through formalin processing does not need AR. Sent from Yahoo Mail on Android On Tue, Jun 20, 2017 at 4:52 PM, Isabel Soto via Histonet wrote: Yes. Sent from Yahoo Mail on Android On Tue, Jun 20, 2017 at 3:15 PM, Nair, Indu via Histonet wrote: Hello Histoneters! question: Does frozen section need Antigen Retrieval? thank you, for the anticipated timely help! indu --------------------------------------------------------------------- -SECURITY/CONFIDENTIALITY WARNING- This message (and any attachments) are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (LCP301) --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw at yahoo.com Tue Jun 20 19:47:41 2017 From: slappycraw at yahoo.com (Larry Woody) Date: Wed, 21 Jun 2017 00:47:41 +0000 (UTC) Subject: [Histonet] frozen sections In-Reply-To: References: <1258283742.2752997.1498001360002@mail.yahoo.com> <2005028927.2802355.1498005035333@mail.yahoo.com> Message-ID: <650099766.2784221.1498006061917@mail.yahoo.com> Then you will need AR. Sent from Yahoo Mail on Android On Tue, Jun 20, 2017 at 5:43 PM, Nair, Indu wrote: The tissue has been fixed in 4% PFA. Thank you! indu ________________________________________ From: Larry Woody via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 20, 2017 5:30 PM To: histonet at lists.utsouthwestern.edu; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] frozen sections Tissue that does not go through formalin processing does not need AR. Sent from Yahoo Mail on Android ? On Tue, Jun 20, 2017 at 4:52 PM, Isabel Soto via Histonet wrote:? Yes. Sent from Yahoo Mail on Android ? On Tue, Jun 20, 2017 at 3:15 PM, Nair, Indu via Histonet wrote: Hello Histoneters! question: Does frozen section need Antigen Retrieval? thank you, for the anticipated timely help! indu --------------------------------------------------------------------- -SECURITY/CONFIDENTIALITY WARNING- This message (and any attachments) are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (LCP301) --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff at uropartners.com Wed Jun 21 07:06:55 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 21 Jun 2017 12:06:55 +0000 Subject: [Histonet] Histo/pathology blog post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF1148978D@COLOEXCH01.uropartners.local> Good morning all: Looking at digital microscopy today on the blog. http://www.chicagonow.com/downsize-maybe/2017/06/will-digital-microscopes-replace-the-real-thing/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From jshelley at sbpdiscovery.org Wed Jun 21 08:05:42 2017 From: jshelley at sbpdiscovery.org (John Shelley) Date: Wed, 21 Jun 2017 13:05:42 +0000 Subject: [Histonet] alpha-glycerophosphate dehydrogenase staining protocol Message-ID: Hi All, I have a group that wants to do an enzyme stain called alpha-glycerophosphate dehydrogenase on mouse skeletal muscle and was wondering if anyone has a working protocol. Thanks!!! Kind Regards! John J Shelley From billodonnell at catholichealth.net Wed Jun 21 08:35:40 2017 From: billodonnell at catholichealth.net (O'Donnell, Bill) Date: Wed, 21 Jun 2017 13:35:40 +0000 Subject: [Histonet] scrubs/business casual Message-ID: Just a little poll. How many histology labs allow scrubs? Of those that are allowed scrubs, do you purchase your own, or are they provided? Bill O'Donnell Good Sameritan Hospital Histology This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From wbenton at cua.md Wed Jun 21 08:58:32 2017 From: wbenton at cua.md (Walter Benton) Date: Wed, 21 Jun 2017 13:58:32 +0000 Subject: [Histonet] scrubs/business casual In-Reply-To: References: Message-ID: Bill, It is a required uniform for our staff. First three sets are provided upon hire. All additional scrubs are purchased by the employee. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates -----Original Message----- From: O'Donnell, Bill via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, June 21, 2017 9:36 AM To: Histonet (histonet at lists.utsouthwestern.edu) Subject: [Histonet] scrubs/business casual Just a little poll. How many histology labs allow scrubs? Of those that are allowed scrubs, do you purchase your own, or are they provided? Bill O'Donnell Good Sameritan Hospital Histology This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From hhuggins at novalabs.co Wed Jun 21 09:28:14 2017 From: hhuggins at novalabs.co (Haley Huggins) Date: Wed, 21 Jun 2017 07:28:14 -0700 Subject: [Histonet] scrubs/business casual In-Reply-To: References: Message-ID: We wear scrubs. However, we are a small reference lab and our employer bought us 4 sets of scrubs w/embroidered company logo to wear M-Th. I added free-scrub Friday, so we can wear whatever scrub set we want to wear on Friday purchased on our own. On Jun 21, 2017 6:51 AM, "O'Donnell, Bill via Histonet" < histonet at lists.utsouthwestern.edu> wrote: Just a little poll. How many histology labs allow scrubs? Of those that are allowed scrubs, do you purchase your own, or are they provided? Bill O'Donnell Good Sameritan Hospital Histology This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas at biopath.org Wed Jun 21 10:31:24 2017 From: plucas at biopath.org (Paula) Date: Wed, 21 Jun 2017 08:31:24 -0700 Subject: [Histonet] Glycophorin Message-ID: <006501d2eaa3$7293e6b0$57bbb410$@biopath.org> Hello all, We have the Leica Bond and I am trying to get the Glycophorin antibody to work on our Bone Marrow Core bx's. Can anyone share the protocol for this antibody? I've tried Enzyme 1, ER 1 for 20 minutes so far. I'm thinking it could be the decal that is interfering but I would like to check with all of you for any suggestions.thanks in advance. Paula From tpodawiltz at yahoo.com Wed Jun 21 11:38:06 2017 From: tpodawiltz at yahoo.com (Thomas Podawiltz) Date: Wed, 21 Jun 2017 16:38:06 +0000 (UTC) Subject: [Histonet] scrubs/business casual In-Reply-To: References: Message-ID: <84136748.3324420.1498063086757@mail.yahoo.com> blockquote, div.yahoo_quoted { margin-left: 0 !important; border-left:1px #715FFA solid !important; padding-left:1ex !important; background-color:white !important; } We allow everything from business casual, scrubs to neat blue jeans. But then again we are on nights. Sent from Yahoo Mail for iPhone On Wednesday, June 21, 2017, 7:33 AM, Haley Huggins via Histonet wrote: We wear scrubs. However, we are a small reference lab and our employer bought us 4 sets of scrubs w/embroidered company logo to wear M-Th. I added free-scrub Friday, so we can wear whatever scrub set we want to wear on Friday purchased on our own. On Jun 21, 2017 6:51 AM, "O'Donnell, Bill via Histonet" < histonet at lists.utsouthwestern.edu> wrote: Just a little poll. How many histology labs allow scrubs? Of those that are allowed scrubs, do you purchase your own, or are they provided? Bill O'Donnell Good Sameritan Hospital Histology This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kristyn.ferber at gmail.com Wed Jun 21 13:57:54 2017 From: kristyn.ferber at gmail.com (Kristyn Ferber) Date: Wed, 21 Jun 2017 14:57:54 -0400 Subject: [Histonet] Histonet Digest, Vol 163, Issue 19 In-Reply-To: References: Message-ID: I would say it is highly dependent on the antibody and application you are using. We don't fix any of our frozen tissue and no antigen retrieval is necessary. However, whenever we receive specimens that have been through a full FFPE process then, yes, the same antibody requires some antigen retrieval. Best, Kristyn > ---------- Forwarded message ---------- > From: "Nair, Indu" > To: "histonet at lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Tue, 20 Jun 2017 22:08:59 +0000 > Subject: [Histonet] frozen sections > > Hello Histoneters! > > question: Does frozen section need Antigen Retrieval? > > thank you, for the anticipated timely help! > > indu > > > From brannon at alliedsearchpartners.com Wed Jun 21 15:40:51 2017 From: brannon at alliedsearchpartners.com (Brannon Owens) Date: Wed, 21 Jun 2017 20:40:51 +0000 Subject: [Histonet] Histotechnician job opening near Hershey, PA Message-ID: Immediate need for a full time/permanent Histotechnician/Histotechnologist on a M-F day shift near Hershey, PA (eastern PA). IHC experience and ASCP certification preferred. Email/call if interested to brannon at alliedsearchpartners.com/(888) 388-7571 ext. 106. Additional opportunities Nationwide just check out the careers page on the Allied Search Partners website ? Happy Hump Day! To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers/ Brannon Owens VP/Director of Recruitment Allied Search Partners LinkedIn: https://www.linkedin.com/in/jbrannonowens http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 Direct Line: 407.413.9421 F: 888.388.7572 From criley at dpspa.com Wed Jun 21 19:53:35 2017 From: criley at dpspa.com (Charles Riley) Date: Wed, 21 Jun 2017 20:53:35 -0400 Subject: [Histonet] Competency Testing Message-ID: Does anyone perform in lab competency testing, not for accreditation purposes but for technical skill levels and staff assessment? If so what skills do you assess and how do you assess them. I am looking to create an assessment for embedding and microtomy but don't know where to set the bar for competency levels. I currently have 1 tech who just graduated a NAACLS accredited program but is uncertified. 1 tech who has been out of the same program for 2 years and certified HT for 1.25 years, 1 tech who is out of the same program for 3 years and certified HT for 3 years, and one job trained tech with 1.5 years experience. If anyone could give me a suggestion as to what they think would be good goals to set at these stages I would greatly appreciate it -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From hhuggins at novalabs.co Thu Jun 22 14:21:04 2017 From: hhuggins at novalabs.co (Haley Huggins) Date: Thu, 22 Jun 2017 12:21:04 -0700 Subject: [Histonet] CPT Code for PGP9.5 free floating tissue stain In-Reply-To: References: Message-ID: I am running into an issue with billing and which code is correct for staining PGP9.5. Is it 88342? Also the billing guy asked me if thr secondary goat, anti-mouse secondary antibody could be billed, can it? I would appreciate any help I can get as soon as possible! He needs to bill out today. Thanks. From william at schuylerhouse.com Thu Jun 22 19:42:55 2017 From: william at schuylerhouse.com (William Shipley) Date: Thu, 22 Jun 2017 17:42:55 -0700 Subject: [Histonet] Quest In-Reply-To: References: Message-ID: My coworker lost her dog. Subsequently she became aware that dogs can be cloned. After three weeks she recovered some samples (yeah). They are currently sitting in cryonic storage awaiting cloning technology to become sufficiently advanced to be able to routinely clone from frozen tissue. It's been done but not commercially. The cryonics people gave her a couple small samples in cryo preservative and suggested she have them sent out for a Histo examination so that there is some record of the nature of the samples currently in storage. Do you know any Histologists who receive canine samples? We are in the Los Angeles area. William Shipley Schuyler House From edmartin26 at gmail.com Fri Jun 23 12:27:58 2017 From: edmartin26 at gmail.com (Eddie Martin) Date: Fri, 23 Jun 2017 13:27:58 -0400 Subject: [Histonet] CPT Code for PGP9.5 free floating tissue stain Message-ID: <5B1C1445-713D-4FDF-8684-643C4E46C4EA@gmail.com> You can only bill the 88342 once for the initial IHC with interpretation charge for the pgp9.5, (assuming it was the first antibody you billed to the patient). The pgp9.5 is the test your pathologist ordered. The linker step in your protocol isn't another test ordered, just part of your validated protocol in order to make the pgp9.5 work correctly. If your pathologist ordered another antibody test on the same specimen part other than pgp9.5, then you could apply the 88341 cpt code. For example, ordering an s-100p on the same specimen would be an 88341 on the same specimen part. However, if you order another pgp9.5 on the same specimen part, then it would be billed as a no charge. Hope this helps. Sent from my iPhone > On Jun 22, 2017, at 3:21 PM, Haley Huggins wrote: > > I am running into an issue with billing and which code is correct for > staining PGP9.5. Is it 88342? Also the billing guy asked me if thr > secondary goat, anti-mouse secondary antibody could be billed, can it? I > would appreciate any help I can get as soon as possible! He needs to bill > out today. Thanks. > From relia1 at earthlink.net Fri Jun 23 13:16:22 2017 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 23 Jun 2017 14:16:22 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin 6/23/2017 Summertime!!!! Message-ID: <001601d2ec4c$d2ba2590$782e70b0$@earthlink.net> Hi Histonetters!! How are you? I hope you have had a great week like me and are ready for a fun and relaxing weekend!! TGIF!!!! The phones haven?t stopped ringing Things are heating up just like the temps! So I thought I would shoot you a quick email and let you know about my latest opportunities I am really excited because these are great opportunities with stable companies experiencing growth, some are RELIA EXCLIUSIVES!!! If you have vacations already planned my clients understand. You won?t have to change your plans if you change jobs. I PROMISE!!!!! So if you have a second grab a patch of shade and a cool beverage and take a second to browse the list of my current openings. Current Histology Opportunities: Irvine, CA Histology Lab Manager San Francisco Bay area Immunohistochemistry Specialist San Francisco Bay area Histotechnician Wilmington, NC Histology Tech Birmingham, AL Night Shift Dermpath Supervisor North of Philadelphia, PA Lead Histotechnologist San Diego, CA Histotechnian ASCP or eligible!! Chattanooga, TN Grossing Histotech Cranford, NJ Histotechnician Glenwood Springs, Co Histotechnician Birmingham, AL Histotechnician All of my clients offer excellent compensation, benefits and some offer relocation assistance and or sign on bonuses. All of these jobs are full time & permanent If you or anyone you know is interested in hearing more about any of these opportunities please contact me. Remember if I place someone you refer to me you will earn a referral bonus. I can be reached ASAP on my cell/text at 407-353-5070 or e-mail me at relia1 at earthlink.net Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From hans at histologistics.com Sun Jun 25 17:36:13 2017 From: hans at histologistics.com (Hans B Snyder) Date: Sun, 25 Jun 2017 18:36:13 -0400 Subject: [Histonet] DakoCytomation Plus pdf Message-ID: Hello, Does anyone have the PDF manual for the DakoCytomation Plus they are willing to share? Looked through the archives, but didn't see anything and can't find online. Thank you Hans B Snyder Histologistics 151 W Main Street Dudley, MA 01571 Lab - 508-461-7207 C- 508-308-7800 hans at histologistics.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From SteveM at mcclainlab.com Mon Jun 26 08:36:11 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Mon, 26 Jun 2017 13:36:11 +0000 Subject: [Histonet] Histonet Digest, Vol 163, Issue 22 CPT Code for PGP9.5 free floating tissue stain (Eddie Martin) In-Reply-To: References: Message-ID: <7774A618-51D1-4BD7-8F80-71D1A4533D9B@mcclainlab.com> Depends also on what you are doing w the pgp9.5 The only reason I know for free floating section technique is for intraepidermal nerve fiber analysis. Morphometric analysis and nerve fiber counting is an additional CPT. Steve A. McClain, MD > On Jun 24, 2017, at 13:09, "histonet-request at lists.utsouthwestern.edu" wrote: > > CPT Code for PGP9.5 free floating tissue stain (Eddie Martin) From DKBoyd at chs.net Mon Jun 26 10:27:26 2017 From: DKBoyd at chs.net (Boyd, Debbie M) Date: Mon, 26 Jun 2017 15:27:26 +0000 Subject: [Histonet] Billing Question Message-ID: <7EAFE982E328304DA6CE2B677BB7624601857419F5@TN001WEXMBX014.US.chs.net> A couple scenarios: 1. FNA with immediate interpretation ( adequacy) and you make 4 cytospins from the remaining material? 2. Three more passes at the same site. What codes are you using? 3. What professional CPT codes are you generating. Thanks. Debbie M. Boyd l Chief Histologist, Anatomic Pathology I Southside Regional Medical Center I 200 Medical Park Blvd. I Petersburg, Va. 23805 I PH: 804-765-5025 I Fax: 804-765-6058 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From JMacDonald at mtsac.edu Mon Jun 26 14:58:57 2017 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Mon, 26 Jun 2017 12:58:57 -0700 Subject: [Histonet] Digital Microscopes Message-ID: Is anyone familiar with digital scanning microscopes? We are in the market for one. Thank you. From c.tague at Pathologyarts.com Tue Jun 27 13:43:41 2017 From: c.tague at Pathologyarts.com (Curt) Date: Tue, 27 Jun 2017 18:43:41 +0000 Subject: [Histonet] Nexus stainers Message-ID: <9C8F910F72893643B3C3793C3D67132B68121054@PATHOLOGYSERVER.pathologyarts.local> Anyone out there using the Nexus and need Trichrome kits? We ordered the wrong kits (2) and naturally Ventana will not let us return them... if you are interested, let me know. Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. From brannon at alliedsearchpartners.com Tue Jun 27 14:43:04 2017 From: brannon at alliedsearchpartners.com (Brannon Owens) Date: Tue, 27 Jun 2017 19:43:04 +0000 Subject: [Histonet] Histology Supervisor job opening in Santa Ana, CA Message-ID: Another new histology leadership opportunity! This one is in Santa Ana, CA just south of LA; the need is for a full time/permanent candidate to work a Tuesday ? Saturday 4am ? 1pm shift. If interested please send an updated resume over to brannon at alliedsearchpartners.com. Also, check out our website for weekly updated job opportunities Nationwide! To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers/ Brannon Owens VP/Director of Recruitment Allied Search Partners LinkedIn: https://www.linkedin.com/in/jbrannonowens/ http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 Direct Line: 407.413.9421 F: 888.388.7572 From Chi.Nguyen-Vo at hhchealth.org Wed Jun 28 12:04:31 2017 From: Chi.Nguyen-Vo at hhchealth.org (Nguyen-Vo, Chi) Date: Wed, 28 Jun 2017 17:04:31 +0000 Subject: [Histonet] Digital Microscopes In-Reply-To: References: Message-ID: <30CF3892CBBC5E41A066A903F356E6D80DBCD236@HHCEXCHMB04.hhcsystem.org> We are looking at Sukura brand. Thanks, Chi -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald at mtsac.edu] Sent: Monday, June 26, 2017 3:59 PM To: Histo List Subject: [Histonet] Digital Microscopes Is anyone familiar with digital scanning microscopes? We are in the market for one. Thank you. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From madeathridge at pastnashville.com Wed Jun 28 13:58:54 2017 From: madeathridge at pastnashville.com (Maryann Deathridge) Date: Wed, 28 Jun 2017 11:58:54 -0700 Subject: [Histonet] cassette printers Message-ID: <4b3f1416dddc42e68307e9c1da617754@pastnashville.com> Histoland- In the market for a cassette printer with barcode capabilities. Tape or ink? Durability? I currently use the tape and never had issues with fading /washing/non-readable. Have heard of some issues with the ink on some units difficult to read esp. with barcodes. Thoughts??? Thanks in advance to responders. madeathridge at pastnashville.com From SteveM at mcclainlab.com Thu Jun 29 04:33:02 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Thu, 29 Jun 2017 09:33:02 +0000 Subject: [Histonet] Original 1979 sales price for Miles Tissue Tek 3000 In-Reply-To: References: Message-ID: <3A6AE158-8CBA-44C2-983F-93F52DE04335@mcclainlab.com> Does Dr. Richmond or anyone else recall the original sales price for the Miles TissueTek VIP 3000? Some of these vacuum processors are still in operation almost 40 years later! Steve A. McClain, MD From Linda.Margraf at cookchildrens.org Thu Jun 29 10:31:46 2017 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Thu, 29 Jun 2017 15:31:46 +0000 Subject: [Histonet] job opportunity in NY Message-ID: Here is a message I am posting for Jennifer. Please don't respond directly to me. Thanks Linda M, Histonet administrator Hello! I would like to post a position on Histonet. The position is at New York Presbyterian Hospital-Weill Cornell. See below. Laboratory Technologist - Immunohistochemistry - Days Advanced Patient Care: Laboratory Technologists Make It Possible Laboratory Technologist - Immunohistochemistry - Days At NewYork-Presbyterian Hospital, Laboratory Technologists are redefining the limits of science and medicine. We study some of the most complex and rarely seen medical conditions - with unmatched energy and expertise. You can help Make It Possible: We're inviting the best laboratory technologists to work side-by-side in our Immunohistochemistry Laboratory NYP/Weill Cornell Medical Center. Here you'll have an opportunity to work with nationally accredited colleagues and contribute to lifesaving results. You'll find real opportunities to maximize your time and training. You'll use the latest and finest technology and techniques. Our implementation of a state of the art Bond Advance/Leica IHC Systems provides results in less than 4 hours. Take on this important role, responsible for optimization and validation of new antibodies as well as maintaining a control library. This position is Monday through Friday from 11:30am to 7:30pm or 8:30am - 4:30pm, based on departmental need. Preferred Criteria * Immunohistochemistry experience * Experience cutting frozen sections for immunofluorescence studies * Computer literacy with strong IT skills * Experience using CoPath * ASCP certification Required Criteria * A Bachelor's degree in Medical Technology or related health science field * New York State Clinical Laboratory Technologist licensure from the New York Education department To apply please visit: http://careers.nyp.org/new-york-jobs/laboratory-technologist-immunohistochemistry-days/00735216#summary Thank you! Jennifer Cianchino Quality Supervisor Immunopathology Department From LisaKennedy at catholichealth.net Thu Jun 29 12:37:16 2017 From: LisaKennedy at catholichealth.net (Kennedy, Lisa) Date: Thu, 29 Jun 2017 17:37:16 +0000 Subject: [Histonet] Histonet Digest, Vol 163, Issue 26 In-Reply-To: References: Message-ID: <517C2A781E654047A5EC98D17390F9F2A9E89915@CHIEX003.CHI.catholichealth.net> Tech called back and stated that she is unable to create the changes we are requesting to the report(s). She stated that she will refer it to the Meditech Custom Report Team. Karen asked that we be notified before any charges would be incurred. -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, June 29, 2017 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 163, Issue 26 CAUTION: This email is not from a CHI source. Only click links or open attachments you know are safe. ...................................................................... Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=YFhW2PYwN3hsZhoCqLOPHsIEIPQ6qDXkZ40AlEYUG9c&r=bHtnEQ-flkxeJeiio_pfetIRWUl20W0DGOyeqBD17zw&m=uIqbYtALnPfXOseNg5qbqiN2K8IQOnbEwhSGdI3D9-c&s=4flyH8MOSk81Dv5X35KDzsoENPWpzZ95NCC8X2L9GfA&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Digital Microscopes (Nguyen-Vo, Chi) 2. cassette printers (Maryann Deathridge) 3. Re: Original 1979 sales price for Miles Tissue Tek 3000 (Steve McClain) 4. job opportunity in NY (Linda Margraf) ---------------------------------------------------------------------- Message: 1 Date: Wed, 28 Jun 2017 17:04:31 +0000 From: "Nguyen-Vo, Chi" To: Jennifer MacDonald , Histo List Subject: Re: [Histonet] Digital Microscopes Message-ID: <30CF3892CBBC5E41A066A903F356E6D80DBCD236 at HHCEXCHMB04.hhcsystem.org> Content-Type: text/plain; charset="us-ascii" We are looking at Sukura brand. Thanks, Chi -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald at mtsac.edu] Sent: Monday, June 26, 2017 3:59 PM To: Histo List Subject: [Histonet] Digital Microscopes Is anyone familiar with digital scanning microscopes? We are in the market for one. Thank you. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ Message: 2 Date: Wed, 28 Jun 2017 11:58:54 -0700 From: "Maryann Deathridge" To: Subject: [Histonet] cassette printers Message-ID: <4b3f1416dddc42e68307e9c1da617754 at pastnashville.com> Content-Type: text/plain; charset="us-ascii" Histoland- In the market for a cassette printer with barcode capabilities. Tape or ink? Durability? I currently use the tape and never had issues with fading /washing/non-readable. Have heard of some issues with the ink on some units difficult to read esp. with barcodes. Thoughts??? Thanks in advance to responders. madeathridge at pastnashville.com ------------------------------ Message: 3 Date: Thu, 29 Jun 2017 09:33:02 +0000 From: Steve McClain To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Original 1979 sales price for Miles Tissue Tek 3000 Message-ID: <3A6AE158-8CBA-44C2-983F-93F52DE04335 at mcclainlab.com> Content-Type: text/plain; charset="us-ascii" Does Dr. Richmond or anyone else recall the original sales price for the Miles TissueTek VIP 3000? Some of these vacuum processors are still in operation almost 40 years later! Steve A. McClain, MD ------------------------------ Message: 4 Date: Thu, 29 Jun 2017 15:31:46 +0000 From: Linda Margraf To: "histonet at lists.utsouthwestern.edu" Cc: "'jmp2008 at nyp.org'" Subject: [Histonet] job opportunity in NY Message-ID: Content-Type: text/plain; charset="us-ascii" Here is a message I am posting for Jennifer. Please don't respond directly to me. Thanks Linda M, Histonet administrator Hello! I would like to post a position on Histonet. The position is at New York Presbyterian Hospital-Weill Cornell. See below. Laboratory Technologist - Immunohistochemistry - Days Advanced Patient Care: Laboratory Technologists Make It Possible Laboratory Technologist - Immunohistochemistry - Days At NewYork-Presbyterian Hospital, Laboratory Technologists are redefining the limits of science and medicine. We study some of the most complex and rarely seen medical conditions - with unmatched energy and expertise. You can help Make It Possible: We're inviting the best laboratory technologists to work side-by-side in our Immunohistochemistry Laboratory NYP/Weill Cornell Medical Center. Here you'll have an opportunity to work with nationally accredited colleagues and contribute to lifesaving results. You'll find real opportunities to maximize your time and training. You'll use the latest and finest technology and techniques. Our implementation of a state of the art Bond Advance/Leica IHC Systems provides results in less than 4 hours. Take on this important role, responsible for optimization and validation of new antibodies as well as maintaining a control library. This position is Monday through Friday from 11:30am to 7:30pm or 8:30am - 4:30pm, based on departmental need. Preferred Criteria * Immunohistochemistry experience * Experience cutting frozen sections for immunofluorescence studies * Computer literacy with strong IT skills * Experience using CoPath * ASCP certification Required Criteria * A Bachelor's degree in Medical Technology or related health science field * New York State Clinical Laboratory Technologist licensure from the New York Education department To apply please visit: https://urldefense.proofpoint.com/v2/url?u=http-3A__careers.nyp.org_new-2Dyork-2Djobs_laboratory-2Dtechnologist-2Dimmunohistochemistry-2Ddays_00735216-23summary&d=DwICAg&c=YFhW2PYwN3hsZhoCqLOPHsIEIPQ6qDXkZ40AlEYUG9c&r=bHtnEQ-flkxeJeiio_pfetIRWUl20W0DGOyeqBD17zw&m=uIqbYtALnPfXOseNg5qbqiN2K8IQOnbEwhSGdI3D9-c&s=dZWpPXLqokgMzR3XNUItiQG_ex-rNOBS1M58tjThuxI&e= Thank you! Jennifer Cianchino Quality Supervisor Immunopathology Department ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=YFhW2PYwN3hsZhoCqLOPHsIEIPQ6qDXkZ40AlEYUG9c&r=bHtnEQ-flkxeJeiio_pfetIRWUl20W0DGOyeqBD17zw&m=uIqbYtALnPfXOseNg5qbqiN2K8IQOnbEwhSGdI3D9-c&s=4flyH8MOSk81Dv5X35KDzsoENPWpzZ95NCC8X2L9GfA&e= ------------------------------ End of Histonet Digest, Vol 163, Issue 26 ***************************************** This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From Douglas.Porter at sparrow.org Thu Jun 29 13:04:02 2017 From: Douglas.Porter at sparrow.org (Porter, Douglas) Date: Thu, 29 Jun 2017 18:04:02 +0000 Subject: [Histonet] Test Post! Message-ID: <5F532A63AECA7B4D86DBD2982E6AC55B67C8E289@EXMBPBAS02.shs.org> Test. Douglas A. Porter, HT (ASCP) Pathologist Assistant IT Coordinator Sparrow Laboratories Department of Pathology 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) douglas.porter at sparrow.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you!