[Histonet] PAX-5

[Tony Henwood (SCHN)]

Hi Beth,
We have found the same thing.

You get a moderate staining (half of what you see with most B cell lymphomas) with clone 1EW (as supplied with the Leica Bond rtu).
You will get similar results with clone 24 (38%:  Adams, et al (2009). Clinical, phenotypic and genetic similarities and disparities between post-transplant and classical Hodgkin lymphomas with respect to therapeutic targets. Expert opinion on therapeutic targets, 13(10), 1137-1145.)

We have found that using the Rabbit monoclonal antibody to Pax-5 (Clone SP34), it seems that, though staining a similar proportion of B cell lymphomas, staining is absent from the Reed-Sternberg cells of Hodgin lymphoma.

Our assessment is in its early stages so with further cases, it might be clearer.

It seems that not all Pax-5 antibodies react the same

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: Linda Margraf via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Saturday, 22 July 2017 7:00 AM
To: histonet at lists.utsouthwestern.edu
Cc: 'oneilb at wvumedicine.org'
Subject: [Histonet] FW: PAX-5

Here is a message I am posting for Beth….


From: "O'neil, Beth" <oneilb at wvumedicine.org<mailto:oneilb at wvumedicine.org>>
Date: July 21, 2017 at 2:31:24 PM CDT
To: "histonet-owner at lists.utsouthwestern.edu<mailto:histonet-owner at lists.utsouthwestern.edu>" <histonet-owner at lists.utsouthwestern.edu<mailto:histonet-owner at lists.utsouthwestern.edu>>
Subject: PAX-5
My pathologists are not happy with PAX-5 on the Ventana Ultra; it will occasionally show cytoplasmic staining of the Reed-Sternberg cells (supposed to be a nuclear stain).  We use Ventana’s clone SP34. I have been trying to optimize with the Optiview kit but we are getting so much background staining and only sporadic staining of the R-S cells.  I tried a different clone, Cell Marque EP156 which was really clean but would not stain the R-S cells at all, no matter what we did.   My Ventana technical specialist is stumped as to why we’re having so much trouble.  Does anyone else have the same problem or have any suggestions?

Beth Ann O’Neil, MT(ASCP)SC, HTL, QIHC
Histology Supervisor, Technical Specialist
Lab:  304 – 293 – 6014
Office:  304 – 293 – 7629
 oneilb at wvumedicine.org<mailto:oneilb at wvumedicine.org>


________________________________


_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities.