From mgiorgi at incdx.com Mon Jul 3 13:38:04 2017 From: mgiorgi at incdx.com (Miranda Giorgi) Date: Mon, 3 Jul 2017 18:38:04 +0000 Subject: [Histonet] Dako Artisan VS. Ventana Benchmark speical stainer Message-ID: Hello, Our lab is currently evaluating the Benchmark Special Stainer from Ventana and we are finding the stain quality and consistency to be lacking, especially the retic and trichrome. While we have the opportunity, we would like to consider an alternative platform, the Dako Artisan. Has anyone used one or both of these plans? If so, can you provide any feedback on your experience, including any pros or cons? Any feedback is very much appreciated as we do not want to be stuck with an instrument that is not going to work. Thanks in advance! Miranda Giorgi, HTL (ASCP)cm This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700. From slappycraw at yahoo.com Mon Jul 3 14:27:48 2017 From: slappycraw at yahoo.com (Larry Woody) Date: Mon, 3 Jul 2017 19:27:48 +0000 (UTC) Subject: [Histonet] Dako Artisan VS. Ventana Benchmark speical stainer In-Reply-To: References: Message-ID: <20387518.4477075.1499110068110@mail.yahoo.com> I've used both platforms and the benchmark is easier to setup but the Artisan has the better stain quality. Just IMO. Sent from Yahoo Mail on Android On Mon, Jul 3, 2017 at 12:06 PM, Miranda Giorgi via Histonet wrote: Hello, Our lab is currently evaluating the Benchmark Special Stainer from Ventana and we are finding the stain quality and consistency to be lacking, especially the retic and trichrome.? While we have the opportunity, we would like to consider an alternative platform, the Dako Artisan.? Has anyone used one or both of these plans?? If so, can you provide any feedback on your experience, including any pros or cons? Any feedback is very much appreciated as we do not want to be stuck with an instrument that is not going to work. Thanks in advance! Miranda Giorgi, HTL (ASCP)cm This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw at yahoo.com Mon Jul 3 14:27:48 2017 From: slappycraw at yahoo.com (Larry Woody) Date: Mon, 3 Jul 2017 19:27:48 +0000 (UTC) Subject: [Histonet] Dako Artisan VS. Ventana Benchmark speical stainer In-Reply-To: References: Message-ID: <20387518.4477075.1499110068110@mail.yahoo.com> I've used both platforms and the benchmark is easier to setup but the Artisan has the better stain quality. Just IMO. Sent from Yahoo Mail on Android On Mon, Jul 3, 2017 at 12:06 PM, Miranda Giorgi via Histonet wrote: Hello, Our lab is currently evaluating the Benchmark Special Stainer from Ventana and we are finding the stain quality and consistency to be lacking, especially the retic and trichrome.? While we have the opportunity, we would like to consider an alternative platform, the Dako Artisan.? Has anyone used one or both of these plans?? If so, can you provide any feedback on your experience, including any pros or cons? Any feedback is very much appreciated as we do not want to be stuck with an instrument that is not going to work. Thanks in advance! Miranda Giorgi, HTL (ASCP)cm This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght at yahoo.com Tue Jul 4 12:55:01 2017 From: tkngflght at yahoo.com (Cheryl) Date: Tue, 4 Jul 2017 12:55:01 -0500 Subject: [Histonet] Seeking a supervisor in Houston Message-ID: <4A3F6BCA-FFCF-4FA2-AD2E-F693CE9F6B03@yahoo.com> Private reference lab -- pathology only -- seeking strong skills and a kind demeanor for a new position. We've grown fast and it's time for an in-department supervisor. Will need to bring some strong skills and grossing experience to support a team of 4 fairly new techs (less than five years on average), a couple of good lab aids and grossers. We have fun every day, do stellar quality work for three lovely, intelligent and engaged pathologists. About to move to new facilities - we need a high performer to meet the next stage of growth. Generous salary, decent hours, relocation stipend for the right candidate. Lots of other benefits and perks (we keep a stocked snack cupboard!!). For consideration, shoot me a resume -- Ckerry at adgpath.com Please excuse typos-sent from a phone. From Scott.Lindrud at rice.willmar.mn.us Wed Jul 5 09:03:31 2017 From: Scott.Lindrud at rice.willmar.mn.us (Lindrud, Scott) Date: Wed, 5 Jul 2017 14:03:31 +0000 Subject: [Histonet] Dako Artisan VS. Ventana Benchmark speical stainer In-Reply-To: References: Message-ID: <5d84cf5be54b4e90a44db431796cabf2@RMH-MAIL.ricehospital.local> Miranda, We use the Dako Artisan for special staining and it performs great. We've been utilizing ours for about 7 years now. We use the following staining protocols: Iron PAS PAS/D GMS Retic Trichrome AFB Warthin Starry The staining is consistent and reproducible for all of our protocols. The stainer is easy to set up and maintain. Ours has been super reliable and when we have had problems, their technical support is helpful. Getting a Field Service Engineer out to make repairs for the few times we've needed it has always been prompt (within a day). I have no experience with the Benchmark so I can't compare that to the Artisan, but I would have to give the Artisan Link a "2 thumbs up" review! Scott Lindrud, MLS(ASCP)CM CTCM Histopathology Technical Specialist Rice Memorial Hospital Willmar, MN -----Original Message----- From: Miranda Giorgi [mailto:mgiorgi at incdx.com] Sent: Monday, July 03, 2017 1:38 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Dako Artisan VS. Ventana Benchmark speical stainer Hello, Our lab is currently evaluating the Benchmark Special Stainer from Ventana and we are finding the stain quality and consistency to be lacking, especially the retic and trichrome. While we have the opportunity, we would like to consider an alternative platform, the Dako Artisan. Has anyone used one or both of these plans? If so, can you provide any feedback on your experience, including any pros or cons? Any feedback is very much appreciated as we do not want to be stuck with an instrument that is not going to work. Thanks in advance! Miranda Giorgi, HTL (ASCP)cm This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700. ________________________________ "This e-mail transmission is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution violates confidentiality and privacy laws and is prohibited. If you are not the intended recipient, please notify the sender immediately and delete all copies of the message. Thank you." From Kelly.Pairan at nationwidechildrens.org Wed Jul 5 11:53:12 2017 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Wed, 5 Jul 2017 16:53:12 +0000 Subject: [Histonet] CD35 Message-ID: <8746d4a9bf994fb4baae8b2e9a22565d@l1perdwmbx01.childrensroot.net> Hi Everyone, I hope you all had a lovely 4th of July. Is anyone out there using a CD35 RTU antibody that they like? The vendor we normally use has had the item on backorder for over a month and a half with no end in sight. Thanks, Kelly Pairan, HTCM(ASCP) From Richard.Cartun at hhchealth.org Wed Jul 5 19:34:11 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Thu, 6 Jul 2017 00:34:11 +0000 Subject: [Histonet] CD35 In-Reply-To: <8746d4a9bf994fb4baae8b2e9a22565d@l1perdwmbx01.childrensroot.net> References: <8746d4a9bf994fb4baae8b2e9a22565d@l1perdwmbx01.childrensroot.net> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E954474A7@HHCEXCHMB03.hhcsystem.org> We are now using Epitomics' clone EPR6602 with great results. I don't know if it's offered as a "RTU", but I would check with Cell Marque or abcam; they carry Epitomics' clones Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Pairan, Kelly via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, July 05, 2017 12:53 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CD35 Hi Everyone, I hope you all had a lovely 4th of July. Is anyone out there using a CD35 RTU antibody that they like? The vendor we normally use has had the item on backorder for over a month and a half with no end in sight. Thanks, Kelly Pairan, HTCM(ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From GauchV at mail.amc.edu Thu Jul 6 07:41:41 2017 From: GauchV at mail.amc.edu (Gauch, Vicki) Date: Thu, 6 Jul 2017 12:41:41 +0000 Subject: [Histonet] Acid Phosphatase Message-ID: Hi, Does anyone have staining procedures for Acid Phosphatase and Alkaline Phosphatase on frozen muscle tissue? If not, can anyone recommend any kits for these stains is available? Any help would be greatly appreciated. Thank you, Vicki Gauch AMCH ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From TNMayer at mdanderson.org Thu Jul 6 10:19:35 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Thu, 6 Jul 2017 15:19:35 +0000 Subject: [Histonet] . Dako Artisan VS. Ventana Benchmark speical stainer Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8839F3ABBD@D1PWPEXMBX08.mdanderson.edu> The Dako is easier and provides better quality. It allows for more user input and if it goes down, the stains can be completed easily due to the reagent packs. The Ventana, in my opinion is for the more novice user. Inexperienced techs, with a mid level volume can easily operate this unit. It is not as easy to complete the stain if the instrument goes down and requires a lot of maintenance. Toysha From: Miranda Giorgi To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Dako Artisan VS. Ventana Benchmark speical stainer Message-ID: Content-Type: text/plain; charset="us-ascii" Hello, Our lab is currently evaluating the Benchmark Special Stainer from Ventana and we are finding the stain quality and consistency to be lacking, especially the retic and trichrome. While we have the opportunity, we would like to consider an alternative platform, the Dako Artisan. Has anyone used one or both of these plans? If so, can you provide any feedback on your experience, including any pros or cons? Any feedback is very much appreciated as we do not want to be stuck with an instrument that is not going to work. Thanks in advance! Miranda Giorgi, HTL (ASCP)cm This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700. The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From Michelle.Dragoo at nm.org Thu Jul 6 12:59:52 2017 From: Michelle.Dragoo at nm.org (Dragoo, Michelle) Date: Thu, 6 Jul 2017 17:59:52 +0000 Subject: [Histonet] . Dako Artisan VS. Ventana Benchmark special stainer Message-ID: <90c469657e28400284fd04c2e6a51670@exc0i44wp.ch.cadhlt.org> I agree with Toysha. I have used both instruments, and the only consistency in staining I could get with the Ventana was inconsistent staining. The DAKO is more user friendly, and stains can be completed if the instrument goes down or stops in the middle of a run. Michelle R. Dragoo, MBA, HT (ASCP) Supervisor, Histology Northwestern Medicine Central Dupage Hospital 25 North Winfield Road, Winfield IL 60190 O: 630.933.6748 michelle.dragoo at nm.org -----Original Message----- From: Mayer,Toysha N [mailto:TNMayer at mdanderson.org] Sent: Thursday, July 06, 2017 10:20 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] . Dako Artisan VS. Ventana Benchmark speical stainer The Dako is easier and provides better quality. It allows for more user input and if it goes down, the stains can be completed easily due to the reagent packs. The Ventana, in my opinion is for the more novice user. Inexperienced techs, with a mid level volume can easily operate this unit. It is not as easy to complete the stain if the instrument goes down and requires a lot of maintenance. Toysha From: Miranda Giorgi To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Dako Artisan VS. Ventana Benchmark speical stainer Message-ID: Content-Type: text/plain; charset="us-ascii" Hello, Our lab is currently evaluating the Benchmark Special Stainer from Ventana and we are finding the stain quality and consistency to be lacking, especially the retic and trichrome. While we have the opportunity, we would like to consider an alternative platform, the Dako Artisan. Has anyone used one or both of these plans? If so, can you provide any feedback on your experience, including any pros or cons? Any feedback is very much appreciated as we do not want to be stuck with an instrument that is not going to work. Thanks in advance! Miranda Giorgi, HTL (ASCP)cm This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700. The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From Linda.Margraf at cookchildrens.org Thu Jul 6 15:17:20 2017 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Thu, 6 Jul 2017 20:17:20 +0000 Subject: [Histonet] Histotech job in Glen Burnie, MD Message-ID: Hi Histonetters, Here is an ad for a job Mike asked me to post to the list. Please don't send inquiries directly to me. Thanks! Linda (Histonet administratIon) We are seeking a full-time Histotechnologist for our Pathology Lab located in Glen Burnie, MD. This position supports the pathology histology laboratory in preparing, processing, and staining of tissue specimens for review by the pathologists. The hours are Monday-Friday from 1:00PM- 9:00PM. Responsibilities: * Responsible for accessioning, grossing, embedding, microtomy, performing H&E and papanicolau staining, and cover slipping of all surgical/cytologic specimen slidesfor microscopic analysis. * Loading the blocks on the processor ensuring quality and timeliness of production. * Modify and validate procedures and methodologies for immunohistochemistry, special staining and other related staining. * Organize cases by matching slides with appropriate paperwork and/or laying slides out in slide folders. * Provide maintenance or troubleshooting of equipment and automated instrumentation within the laboratory. * Assists supervisor in meeting department regulatory agency requirements (CAP, CLIA, etc.) * Performs quality control procedures per protocol and completes necessary documentation. * Maintain supplies and other inventory. * Perform other laboratory and office duties as assigned by manager. Requirements * An Associate's Degree in a laboratory science or medical laboratory technology obtained from an accredited institution or education/training equivalent to the above that includes at least 60 semester hours from an accredited institution. * HT or HTL certification required. * Proficient embedding, sectioning and histology techniques with human tissues Interested applicants should complete an application at http://www.chesapeakeurology.com/about/employment/apply/. Thanks so much. From histocorp at gmail.com Thu Jul 6 20:49:21 2017 From: histocorp at gmail.com (Stephen D'Alessandro) Date: Fri, 07 Jul 2017 01:49:21 +0000 Subject: [Histonet] Histotech job in Glen Burnie, MD In-Reply-To: References: Message-ID: On Thu, Jul 6, 2017 at 3:25 PM Linda Margraf via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Histonetters, > > Here is an ad for a job Mike asked me to post to the list. Please don't > send inquiries directly to me. Thanks! > Linda (Histonet administratIon) > > We are seeking a full-time Histotechnologist for our Pathology Lab located > in Glen Burnie, MD. This position supports the pathology histology > laboratory in preparing, processing, and staining of tissue specimens for > review by the pathologists. The hours are Monday-Friday from 1:00PM- 9:00PM. > > Responsibilities: > > * Responsible for accessioning, grossing, embedding, microtomy, > performing H&E and papanicolau staining, and cover slipping of all > surgical/cytologic specimen slidesfor microscopic analysis. > > * Loading the blocks on the processor ensuring quality and > timeliness of production. > > * Modify and validate procedures and methodologies for > immunohistochemistry, special staining and other related staining. > > * Organize cases by matching slides with appropriate paperwork > and/or laying slides out in slide folders. > > * Provide maintenance or troubleshooting of equipment and > automated instrumentation within the laboratory. > > * Assists supervisor in meeting department regulatory agency > requirements (CAP, CLIA, etc.) > > * Performs quality control procedures per protocol and completes > necessary documentation. > > * Maintain supplies and other inventory. > > * Perform other laboratory and office duties as assigned by > manager. > > Requirements > > * An Associate's Degree in a laboratory science or medical > laboratory technology obtained from an accredited institution or > education/training equivalent to the above that includes at least 60 > semester hours from an accredited institution. > > * HT or HTL certification required. > > * Proficient embedding, sectioning and histology techniques with > human tissues > > Interested applicants should complete an application at > http://www.chesapeakeurology.com/about/employment/apply/< > https://urldefense.proofpoint.com/v2/url?u=http-3A__www.chesapeakeurology.com_about_employment_apply_&d=DwMFAg&c=fHUKEfYp8ZKZp-Z4zyr9bXWWb-JCctOum5ZlzbjkVjM&r=1o7iZWfSCXEgDesmDMuV2rPUZJJnqv0nrvyFk2x0v0A&m=KsB9C62OJ2FKr93urrbmgUoyUWqcjQCSd8p2nzLITjM&s=fbm_Nk6KXS7cjjdJepAFezdU4nxxyS_ndUASKUgG__4&e=>. > Thanks so much. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Karoleigh.Armstrong at ARMC.net Fri Jul 7 11:10:29 2017 From: Karoleigh.Armstrong at ARMC.net (Armstrong, Karoleigh T) Date: Fri, 7 Jul 2017 16:10:29 +0000 Subject: [Histonet] O fix IHC Message-ID: Hey guys How long do most people fix in O Fix (We use it as a secondary fixative). Do you have issues with IHC staining, other than ER and PR Her2, interference? -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From sandra.cheasty at wisc.edu Fri Jul 7 14:33:36 2017 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Fri, 07 Jul 2017 19:33:36 +0000 Subject: [Histonet] Pathology Band Saw Message-ID: Hello, Can anyone recommend a band saw for pathology? We are looking for a new band saw for use in necropsy and research. It has to be a "meat" band saw, as workshop band saws aren't compatible with wet tissue. A table-top model is preferred, and cheaper than a floor model. (Plus, the floor models all seem to come with a sausage-maker, which is pretty gross.) If you have used a table-top band saw in your pathology lab, I'd appreciate hearing from you. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From areichard at lmhealth.org Mon Jul 10 08:53:12 2017 From: areichard at lmhealth.org (Amanda Reichard) Date: Mon, 10 Jul 2017 13:53:12 +0000 Subject: [Histonet] Leica SelecTech Stains Message-ID: <810cc85324744c6abf45df0e1df9344f@LMH-EXMB-1.lmhealth.org> For those of you using the SelecTech stains... Do you use the entire system (define and blue buffer), or just the hematoxylin and eosin? It seems from previous posts everyone who has used it is very happy with it, I'm just curious if we can stick with a less pricey differentiation and bluing solution. Thanks in advance Amanda Reichard, HTL (ASCP)cm Histology/Cytology Supervisor Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (220) 564-4163 areichard at lmhealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 220-564-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From angie at ka-recruiting.com Mon Jul 10 08:54:33 2017 From: angie at ka-recruiting.com (Angie Laparidis) Date: Mon, 10 Jul 2017 09:54:33 -0400 Subject: [Histonet] Louisiana Histotech Opening Message-ID: Dear Histonet Users, I wanted to share a brand new opening with a well-known laboratory in the Lafayette, LA area! They are looking for a Full-time histotech to join their expanding laboratory. Hours are around 2am-10am Tuesday-Friday morning, then from 10pm-6am on Friday night/Sat. morning. This is an ideal opportunity if you're a tech that prefers early mornings! This laboratory offers great compensation and benefits packages and the opening should not be available for very long. Feel free to send over a resume and let me know if you would like more details! Sincerely, *Angie Laparidis*Healthcare Recruiter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South, Boston, MA, 02109 W: 617.746-2744 (*please note this is a new number*) F: (617) 507-8009 Angie at ka-recruiting.com Our openings are updated daily at www.ka-recruiting.com From cahill at mit.edu Mon Jul 10 09:57:29 2017 From: cahill at mit.edu (Lucas Cahill) Date: Mon, 10 Jul 2017 10:57:29 -0400 Subject: [Histonet] Fresh tissue soaked in ethanol solution prior to formalin fixation Message-ID: Hi all, I am using a protocol where fresh breast tissue is immersed in an alcohol based staining solution (50% ethanol, 50% water) for 2-5 minutes before formalin fixation and paraffin processing for H&E, immunohistochemistry, and immunofluorescence. I have not seen differences in subsequent processing on the tissue that I've tested, however, I'm wondering if the 50% ethanol solution on fresh tissue has an effect. I know tissue can be alcohol fixed but this is not standard in clinical breast tissue processing and 2-5 mins does not seem like it would fix the tissue. Are there any protocols that use an alcohol based solution on fresh tissue prior to formalin fixation that are known not to affect subsequent processing such as immunohistochemistry? Does anyone have any intuition on how this could affect tissue analysis? Best, Lucas -- *Lucas Cahill* PhD Candidate | Medical Engineering & Medical Physics Harvard-MIT Health Sciences & Technology From Timothy.Morken at ucsf.edu Mon Jul 10 10:19:55 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 10 Jul 2017 15:19:55 +0000 Subject: [Histonet] Fresh tissue soaked in ethanol solution prior to formalin fixation In-Reply-To: References: Message-ID: Luca, sounds like a project to figure that out! Anytime you soak the tissue in any percentage alcoholic solution there is going to be some dehydration - substitution of water with alcohol. When adding alcohol you will cause precipitation of some of the proteins in the tissue - depends on the alcohol concentration. This does not normally affect immuno or other staining, but can cause some morphological artifacts from the wave effect of the alcohol moving thru the tissue. Then if you put back in aqueous formalin, that rehydrate the tissue which may induce other artifacts. A better idea may be to use alcoholic formalin for the rest of the fixation in the same concentration. However, alcoholic formalin is normally used in a tissue processor after normal fixation with aqueous formalin at which time the tissue should be fixed enough to avoid the morphological changes. It is an attempt to start the dehydration process earlier in the cycle. If the tissue is small, or thin - less than 5mm - it will alcohol fix all the way thru. If larger you could get alcohol fixation artifacts in some areas and not in others where the alcohol does not reach. What is the staining solution supposed to accomplish as a pre-fixation technique that can't be done later in the process? An out of the ordinary process like this requires some test studies to see how it affects whatever it is you want to see, in parallel with normal methods. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Lucas Cahill via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, July 10, 2017 7:57 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fresh tissue soaked in ethanol solution prior to formalin fixation Hi all, I am using a protocol where fresh breast tissue is immersed in an alcohol based staining solution (50% ethanol, 50% water) for 2-5 minutes before formalin fixation and paraffin processing for H&E, immunohistochemistry, and immunofluorescence. I have not seen differences in subsequent processing on the tissue that I've tested, however, I'm wondering if the 50% ethanol solution on fresh tissue has an effect. I know tissue can be alcohol fixed but this is not standard in clinical breast tissue processing and 2-5 mins does not seem like it would fix the tissue. Are there any protocols that use an alcohol based solution on fresh tissue prior to formalin fixation that are known not to affect subsequent processing such as immunohistochemistry? Does anyone have any intuition on how this could affect tissue analysis? Best, Lucas -- *Lucas Cahill* PhD Candidate | Medical Engineering & Medical Physics Harvard-MIT Health Sciences & Technology _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper at chla.usc.edu Mon Jul 10 10:40:10 2017 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Mon, 10 Jul 2017 15:40:10 +0000 Subject: [Histonet] Leica SelecTech Stains In-Reply-To: <810cc85324744c6abf45df0e1df9344f@LMH-EXMB-1.lmhealth.org> References: <810cc85324744c6abf45df0e1df9344f@LMH-EXMB-1.lmhealth.org> Message-ID: Hi Amanda, We used to use their Define Solution, but now we simply stain with their Hematoxylin 560 MX and Alcoholic Eosin-Y. 1% acid alcohol (5 quick dips) for differentiation and dilute ammonia water for bluing. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357???? Pager: 213-209-0184 bcooper at chla.usc.edu -----Original Message----- From: Amanda Reichard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, July 10, 2017 6:53 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Leica SelecTech Stains (EXTERNAL EMAIL) For those of you using the SelecTech stains... Do you use the entire system (define and blue buffer), or just the hematoxylin and eosin? It seems from previous posts everyone who has used it is very happy with it, I'm just curious if we can stick with a less pricey differentiation and bluing solution. Thanks in advance Amanda Reichard, HTL (ASCP)cm Histology/Cytology Supervisor Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (220) 564-4163 areichard at lmhealth.org ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 220-564-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From nguy0515 at gmail.com Mon Jul 10 13:01:14 2017 From: nguy0515 at gmail.com (Trini Nguyen) Date: Mon, 10 Jul 2017 13:01:14 -0500 Subject: [Histonet] Humidity issues-desperate for help Message-ID: Hello Histonetters! I am currently working in a Mohs lab in Minnesota and during the winter time we have no issues with humidity but comes summer, my slides get washed out by the end of the day. I have tried everything I can think of but NOTHING works. I have tried changing solutions throughout the day, keeping the stainer lid open, adding molecular sieve in my alcohols and xylene substitutes, and a dehumidifier. We have the mini Thermo linear stainer under an airfiltronix filter which adds to the humidity issue. If anybody out there has other solutions I haven't tried yet please send them my way! One more thing that I have heard a long time ago at a conference is that if you use xylene substitutes then you do not have to use a hood/any type of ventilation system. Does that sound correct to anyone? Is that statement true? I have worked in labs that coverslip straight from alcohol and a ventilation system is not required but I notice the staining is not optimal in the long run. From lmarie08 at uga.edu Mon Jul 10 14:31:52 2017 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Mon, 10 Jul 2017 19:31:52 +0000 Subject: [Histonet] gloves Message-ID: Hello HistoWorld, My lab is desperately trying to find where to purchase "Cranberry Aqua Source Nitrile Gloves" and having no luck. Going to the website listed on the box of gloves, there is no way to purchase the gloves online. It just says 'to order contact your local dealer'. I called their 800 number and they said that you have to contact a distributer and they recommended Henry Schien. When I contacted Henry Schien they were very snobbish on the phone and said that there was no way to get a quote from them if we aren't already "members" and account holders with Henry Schien. Just thought I would ask fellow histotechs if they use these gloves and if so, who their distributer is. Thanks! :) From relia1 at earthlink.net Tue Jul 11 09:56:17 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 11 Jul 2017 10:56:17 -0400 Subject: [Histonet] It's A Sizzling Hot Summer And It's Not Just the Temps!! RELIA Histology Careers Bulletin 7-11-2017 Message-ID: <020901d2fa55$d98d3580$8ca7a080$@earthlink.net> Hi Histonetters!! I hope you are having a great day. Are you keeping cool on these hot summer days? It?s Summer time and it?s Sizzlin? Hot and It's not just the temps!! I have some great new opportunities to tell you about: o I have full time positions with the best employers in the country. o Competitive salaries, great benefits & relocation assistance. o ASCP or eligible; experienced or entry level!! o My clients are ready to interview and hire today!! They are waiting to hear from me About candidates like you and your friends!! Here are my BRAND NEW Opportunities: Pomona, CA ? Run your own BRAND NEW LAB!! Ventura, CA ? Run your own BRAND NEW LAB!! Irvine, CA ? Lead Histotech ?Oppty for advancement! San Diego, CA ? Grossing Histotechnologist BRAND NEW LAB!! Lafayette, LA ? Histotech day and night shift available! Here is a list of the Sizzlin Summer Stuff! ? Pomona, CA ? Run your own BRAND NEW LAB!! ? Ventura, CA ? Run your own BRAND NEW LAB!! ? Irvine, CA ? Lead Histotech ?Oppty for advancement! ? Modesto, CA - IHC Histotech ? Modesto, CA - Histology Tech ? San Diego, CA ? Grossing Histotechnologist Brand New Lab!! ? Tyler, TX - Senior Histology Tech ? Cranford, NJ- Histology Tech ? Chattanooga, TN- Grossing Histotechnologist ? Lafayette, LA ? Histotech day and night shift available! If you have any questions or know someone who might be interested please contact me. I can be reached toll free at the office at 866-607-3542, email me at relia1 at earthlink.net or call/text me on my cell at 407-353-5070. I would love to tell you about these opportunities or write another referral check like I did today if you have a friend who might be interested. I hope to hear from you soon. In the meantime grab some shade and a cool drink! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From hans at histologistics.com Tue Jul 11 13:41:36 2017 From: hans at histologistics.com (Hans B Snyder) Date: Tue, 11 Jul 2017 14:41:36 -0400 Subject: [Histonet] Histonet Digest, Vol 164, Issue 7 In-Reply-To: References: Message-ID: Hello "Humidity issues-desperate for help", We have this problem every year during the summer months. Our fix is to bottle all chemicals when not in use. We have a plethora of 1 liter nalgene glass bottles we use for chemical solutions. It works for us. Thank you Hans B Snyder Histologistics Inc. 151 W Main Street Dudley, MA 01571 Lab - 508-461-7207 C- 508-308-7800 www.histologistics.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. On Tue, Jul 11, 2017 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Humidity issues-desperate for help (Trini Nguyen) > 2. gloves (Lauren Sweeney) > 3. It's A Sizzling Hot Summer And It's Not Just the Temps!! > RELIA Histology Careers Bulletin 7-11-2017 (Pam Barker) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 10 Jul 2017 13:01:14 -0500 > From: Trini Nguyen > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Humidity issues-desperate for help > Message-ID: > gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hello Histonetters! > > I am currently working in a Mohs lab in Minnesota and during the winter > time we have no issues with humidity but comes summer, my slides get washed > out by the end of the day. I have tried everything I can think of but > NOTHING works. > > I have tried changing solutions throughout the day, keeping the stainer lid > open, adding molecular sieve in my alcohols and xylene substitutes, and a > dehumidifier. > > We have the mini Thermo linear stainer under an airfiltronix filter which > adds to the humidity issue. > > If anybody out there has other solutions I haven't tried yet please send > them my way! > > One more thing that I have heard a long time ago at a conference is that if > you use xylene substitutes then you do not have to use a hood/any type of > ventilation system. Does that sound correct to anyone? Is that statement > true? > > I have worked in labs that coverslip straight from alcohol and a > ventilation system is not required but I notice the staining is not optimal > in the long run. > > > ------------------------------ > > Message: 2 > Date: Mon, 10 Jul 2017 19:31:52 +0000 > From: Lauren Sweeney > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] gloves > Message-ID: > namprd02.prod.outlook.com> > > Content-Type: text/plain; charset="us-ascii" > > Hello HistoWorld, > > My lab is desperately trying to find where to purchase "Cranberry Aqua > Source Nitrile Gloves" and having no luck. Going to the website listed on > the box of gloves, there is no way to purchase the gloves online. It just > says 'to order contact your local dealer'. I called their 800 number and > they said that you have to contact a distributer and they recommended Henry > Schien. When I contacted Henry Schien they were very snobbish on the phone > and said that there was no way to get a quote from them if we aren't > already "members" and account holders with Henry Schien. Just thought I > would ask fellow histotechs if they use these gloves and if so, who their > distributer is. > > Thanks! :) > > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 11 Jul 2017 10:56:17 -0400 > From: "Pam Barker" > To: "Histonetters Histonet" > Subject: [Histonet] It's A Sizzling Hot Summer And It's Not Just the > Temps!! RELIA Histology Careers Bulletin 7-11-2017 > Message-ID: <020901d2fa55$d98d3580$8ca7a080$@earthlink.net> > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonetters!! > I hope you are having a great day. > Are you keeping cool on these hot summer days? > It?s Summer time and it?s Sizzlin? Hot > and > It's not just the temps!! > I have some great new opportunities to tell you about: > o I have full time positions with the best employers in the country. > o Competitive salaries, great benefits & relocation assistance. > o ASCP or eligible; experienced or entry level!! > o My clients are ready to interview and hire today!! > > They are waiting to hear from me > About candidates like you and your friends!! > > Here are my BRAND NEW Opportunities: > Pomona, CA ? Run your own BRAND NEW LAB!! > Ventura, CA ? Run your own BRAND NEW LAB!! > Irvine, CA ? Lead Histotech ?Oppty for advancement! > San Diego, CA ? Grossing Histotechnologist BRAND NEW LAB!! > Lafayette, LA ? Histotech day and night shift available! > > Here is a list of the Sizzlin Summer Stuff! > ? Pomona, CA ? Run your own BRAND NEW LAB!! > ? Ventura, CA ? Run your own BRAND NEW LAB!! > ? Irvine, CA ? Lead Histotech ?Oppty for advancement! > ? Modesto, CA - IHC Histotech > ? Modesto, CA - Histology Tech > ? San Diego, CA ? Grossing Histotechnologist Brand New Lab!! > ? Tyler, TX - Senior Histology Tech > ? Cranford, NJ- Histology Tech > ? Chattanooga, TN- Grossing Histotechnologist > ? Lafayette, LA ? Histotech day and night shift available! > > If you have any questions or know someone who might be interested please > contact me. > I can be reached toll free at the office at 866-607-3542, email me at > relia1 at earthlink.net or call/text me on my cell at 407-353-5070. > I would love to tell you about these opportunities or write another > referral > check like I did today if you have a friend who might be interested. I > hope > to hear from you soon. > In the meantime grab some shade and a cool drink! > > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > ?Pam M. Barker > ? > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell:???? (407)353-5070 > FAX:???? (407)678-2788 > E-mail: relia1 at earthlink.net > www.facebook.com/PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > > > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 164, Issue 7 > **************************************** > From mgunderson at siennadiagnostics.com.au Tue Jul 11 17:30:15 2017 From: mgunderson at siennadiagnostics.com.au (Michael Gunderson) Date: Tue, 11 Jul 2017 22:30:15 +0000 Subject: [Histonet] Use of hTERT for UCC Diagnosis Message-ID: Hello, I work for Sienna Cancer Diagnostics, the company that manufactures the only FDA approved IVD antibody (clone SCD-A7) for hTERT in the United States. We recently entered the US market and are doing so far. What I am looking for now are Urologists that have ordered the hTERT antibody to help diagnose urothelial cell carcinoma and speak to its usefulness in doing so. Additionally, if you're interested at all in using our antibody in your lab I'd happily any questions you may have. If you would be willing to provide a few quotes or know of someone that can speak to the validity of hTERT staining please reach out to me at your earliest convenience. Thank you. .......................................................................................... Michael A. Gunderson HTL(ASCP) Commercial Manager-USA Sienna Cancer Diagnostics 1150 Dorothea Drive, Zumbrota Minnesota 55992 M +1 (507)-884-1725 www.siennadiagnostics.com.au .......................................................................................... IMPORTANT NOTE: This message and any attachments may contain privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you are hereby notified that any use, distribution or reproduction of this message and any attachments is prohibited. Although we use virus scanning software, we deny all liability for viruses or alike in any message or attachment. If you have received this message in error please contact the sender immediately. From cahill at mit.edu Tue Jul 11 21:02:29 2017 From: cahill at mit.edu (Lucas Cahill) Date: Tue, 11 Jul 2017 22:02:29 -0400 Subject: [Histonet] Fresh tissue soaked in ethanol solution prior to formalin fixation In-Reply-To: References: Message-ID: Hi Tim, Thanks for the insight! The staining solution is for some experiments on labeling of unfixed tissue for fluorescence microscopy so I need to immerse the tissue in the solution prior to formalin. The labels I am using (rhodamine) are more soluble in alcohol than water so I would like to use them in alcohol. Lucas On Mon, Jul 10, 2017 at 11:19 AM, Morken, Timothy wrote: > Luca, sounds like a project to figure that out! > > Anytime you soak the tissue in any percentage alcoholic solution there is > going to be some dehydration - substitution of water with alcohol. > > When adding alcohol you will cause precipitation of some of the proteins > in the tissue - depends on the alcohol concentration. This does not > normally affect immuno or other staining, but can cause some morphological > artifacts from the wave effect of the alcohol moving thru the tissue. > > Then if you put back in aqueous formalin, that rehydrate the tissue which > may induce other artifacts. A better idea may be to use alcoholic formalin > for the rest of the fixation in the same concentration. However, alcoholic > formalin is normally used in a tissue processor after normal fixation with > aqueous formalin at which time the tissue should be fixed enough to avoid > the morphological changes. It is an attempt to start the dehydration > process earlier in the cycle. > > If the tissue is small, or thin - less than 5mm - it will alcohol fix all > the way thru. If larger you could get alcohol fixation artifacts in some > areas and not in others where the alcohol does not reach. > > What is the staining solution supposed to accomplish as a pre-fixation > technique that can't be done later in the process? > > An out of the ordinary process like this requires some test studies to see > how it affects whatever it is you want to see, in parallel with normal > methods. > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > -----Original Message----- > From: Lucas Cahill via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Monday, July 10, 2017 7:57 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Fresh tissue soaked in ethanol solution prior to > formalin fixation > > Hi all, > > I am using a protocol where fresh breast tissue is immersed in an alcohol > based staining solution (50% ethanol, 50% water) for 2-5 minutes before > formalin fixation and paraffin processing for H&E, immunohistochemistry, > and immunofluorescence. I have not seen differences in subsequent > processing on the tissue that I've tested, however, I'm wondering if the > 50% ethanol solution on fresh tissue has an effect. I know tissue can be > alcohol fixed but this is not standard in clinical breast tissue processing > and 2-5 mins does not seem like it would fix the tissue. > Are there any protocols that use an alcohol based solution on fresh tissue > prior to formalin fixation that are known not to affect subsequent > processing such as immunohistochemistry? Does anyone have any intuition on > how this could affect tissue analysis? > > Best, > > Lucas > > > -- > *Lucas Cahill* > PhD Candidate | Medical Engineering & Medical Physics Harvard-MIT Health > Sciences & Technology _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *Lucas Cahill* PhD Candidate | Medical Engineering & Medical Physics Harvard-MIT Health Sciences & Technology P: 617-792-0746 From bakevictoria at gmail.com Tue Jul 11 21:23:55 2017 From: bakevictoria at gmail.com (Victoria Baker) Date: Tue, 11 Jul 2017 22:23:55 -0400 Subject: [Histonet] For NYS laboratories In-Reply-To: References: Message-ID: I'm trying to reach out to other NYS Histology labs. I can't really get a real answer regarding a non-CLT doing the embedding/microtomy and staining of just Autopsy material. I think that they can't but the actual documentation is not completely clear. What are other labs doing? Thank you in advance for any information. Vikki From thigginsht at msn.com Wed Jul 12 09:43:06 2017 From: thigginsht at msn.com (T H) Date: Wed, 12 Jul 2017 14:43:06 +0000 Subject: [Histonet] Fungus Control Blocks Message-ID: Hello Histonetters, Does anyone have FUNGUS control blocks they would be willing to share or trade for some other type of control blocks. I have some really good AFB control blocks or Hpylori blocks if anyone is interested. Thanks, Tim From greg.dobbin at gmail.com Wed Jul 12 10:08:48 2017 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Wed, 12 Jul 2017 12:08:48 -0300 Subject: [Histonet] Section adherence issues (IHC) Message-ID: Hi Folks, I haven't been on here much lately but it is nice to know that we are all here for each other when needed! My problem is section adherence during IHC staining. And it is not all of the time it is intermittent; so not all of the time and not on all slides when it does occur. Background: We have a Bond-III immunostainer and we use the Leica Apex charged slides for our IHC stains. We have no additives in our water baths. Our sections drain in a stand and then any trapped water under the section is either flicked or wicked away as needed prior to placing the slides in a rack in a 60 C oven for 30 mins prior to staining. We do not do on board baking (primarily because on board baking is only 10 mins long and with the section lifting issue we want longer). Some of the specimen types are more susceptible it would seem. Cervix LEEP specimens tend to be bad, we use a 4mm punch to obtain some of our control tissue and so the breast tissue we use for Myosin Heavy Chain seems to be a bad one and sometimes our ER/PR control sections (but not always). Fine needle cores where a lot of tumour is present tend to be bad (again not always). We have tried baking longer, we turn ourselves inside out trying to get all of the water out from under the sections, we have tried charged slides from another manufacturer. I have looked at the HIER protocols and none are extraordinary in nature. We use very clean covertiles (no scratches or blemishes). Our specimens are fixed for 24hrs before processing. We use the same slides for Special Stains and don't have this issue there. I need some new suggestions to try! All ideas welcome. Cheers, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From wbenton at cua.md Wed Jul 12 10:33:58 2017 From: wbenton at cua.md (Walter Benton) Date: Wed, 12 Jul 2017 15:33:58 +0000 Subject: [Histonet] Section adherence issues (IHC) In-Reply-To: References: Message-ID: <49f64458e4ef446d85c39a3e4aefb71d@MAIL01.GCU-MD.local> Greg, We are running into a similar issue on the Biocare Nemesis platform and the support there suggest humidity to be the issue. Our sections stay adhered to the slides through depar, antigen retrieval, but appear to fall off during the staining process. It is not consistent. We are going to try some other slides and see if the issue persists. I'd be interested to hear your findings and resolution. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 -----Original Message----- From: Greg Dobbin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, July 12, 2017 11:09 AM To: histonet at lists.utsouthwestern.edu Cc: kgeveleigh at ihis.org Subject: [Histonet] Section adherence issues (IHC) Hi Folks, I haven't been on here much lately but it is nice to know that we are all here for each other when needed! My problem is section adherence during IHC staining. And it is not all of the time it is intermittent; so not all of the time and not on all slides when it does occur. Background: We have a Bond-III immunostainer and we use the Leica Apex charged slides for our IHC stains. We have no additives in our water baths. Our sections drain in a stand and then any trapped water under the section is either flicked or wicked away as needed prior to placing the slides in a rack in a 60 C oven for 30 mins prior to staining. We do not do on board baking (primarily because on board baking is only 10 mins long and with the section lifting issue we want longer). Some of the specimen types are more susceptible it would seem. Cervix LEEP specimens tend to be bad, we use a 4mm punch to obtain some of our control tissue and so the breast tissue we use for Myosin Heavy Chain seems to be a bad one and sometimes our ER/PR control sections (but not always). Fine needle cores where a lot of tumour is present tend to be bad (again not always). We have tried baking longer, we turn ourselves inside out trying to get all of the water out from under the sections, we have tried charged slides from another manufacturer. I have looked at the HIER protocols and none are extraordinary in nature. We use very clean covertiles (no scratches or blemishes). Our specimens are fixed for 24hrs before processing. We use the same slides for Special Stains and don't have this issue there. I need some new suggestions to try! All ideas welcome. Cheers, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From relia1 at earthlink.net Wed Jul 12 10:44:01 2017 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 12 Jul 2017 11:44:01 -0400 Subject: [Histonet] Exciting Opportunities in California!! Can you Help? (Most are RELIA EXCLUSIVES)!! Message-ID: <070701d2fb25$aeee72f0$0ccb58d0$@earthlink.net> Hi Histonetters, How are you? I hope you are having a great week!! I am presently on searches for some of my best clients in California that are in need of histology professionals. Can you help? **Most of these positions are RELIA EXCLUSIVES** My clients offer excellent compensation, benefits, relocation assistance and environments that are great to work in. These are permanent full time positions in busy growing private labs. Here are my most EXCITING OPPORTUNITIES: ? Irvine, CA - Histotechnician ? AWESOME PLACE TO WORK! ? Irvine, CA - Lead Histology Tech ? Irvine, CA - Histology Lab Manager ? Modesto, CA ? IHC Specialist ? state of the art lab! ? Modesto, CA ? ASCP or eligible and Entry Level !! ? San Diego, CA- CLIA qual to Gross ? New Lab ? Pomona, CA ? Run your own brand new lab!! ? Ventura, CA - Run your own brand new lab!! The help I need is do you know anyone that might be interested in hearing about any of these opportunities? If so could you please forward my e-mail to them? If you are interested in any of these positions please call me ASAP at 866-607-3542 or on my cell/text at 407-353-5070 or e-mail me at relia1 at earthlink.net Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From wbenton at cua.md Wed Jul 12 12:37:38 2017 From: wbenton at cua.md (Walter Benton) Date: Wed, 12 Jul 2017 17:37:38 +0000 Subject: [Histonet] Section adherence issues (IHC) In-Reply-To: References: <49f64458e4ef446d85c39a3e4aefb71d@MAIL01.GCU-MD.local> Message-ID: <816e042155824c4193f79c50c1461b48@MAIL01.GCU-MD.local> Thanks. This is helpful information. -----Original Message----- From: Hujet, Matthew [mailto:Matthew.Hujet at ssmhealth.com] Sent: Wednesday, July 12, 2017 1:35 PM To: Walter Benton ; Greg Dobbin Cc: kgeveleigh at ihis.org; histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] Section adherence issues (IHC) We have had really good luck with both the TOMO and Millennia 2000 adhesion slides. We initially tried using the TOMO slides for everything (IHC, special stains, and H&E's), but found that there was too much background staining on the specials and H&E's. For IHC, the TOMO slides were great. We then switched to the Millennia 2000 for everything, and they have been working really well across the board. With regards to just IHC, I personally feel that the TOMO slides are better, but if you only want one type of glass in your lab, the Millennia 2000 are the way to go. Matthew Hujet Histology Technical Specialist SSM Health St. Mary's Hospital - Madison 700 S. Park St. Madison, WI 53715 Please note my new email address: Matthew.Hujet at ssmhealth.com -----Original Message----- From: Walter Benton [mailto:wbenton at cua.md] Sent: Wednesday, July 12, 2017 10:34 AM To: Greg Dobbin Cc: kgeveleigh at ihis.org; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Section adherence issues (IHC) Greg, We are running into a similar issue on the Biocare Nemesis platform and the support there suggest humidity to be the issue. Our sections stay adhered to the slides through depar, antigen retrieval, but appear to fall off during the staining process. It is not consistent. We are going to try some other slides and see if the issue persists. I'd be interested to hear your findings and resolution. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 -----Original Message----- From: Greg Dobbin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, July 12, 2017 11:09 AM To: histonet at lists.utsouthwestern.edu Cc: kgeveleigh at ihis.org Subject: [Histonet] Section adherence issues (IHC) Hi Folks, I haven't been on here much lately but it is nice to know that we are all here for each other when needed! My problem is section adherence during IHC staining. And it is not all of the time it is intermittent; so not all of the time and not on all slides when it does occur. Background: We have a Bond-III immunostainer and we use the Leica Apex charged slides for our IHC stains. We have no additives in our water baths. Our sections drain in a stand and then any trapped water under the section is either flicked or wicked away as needed prior to placing the slides in a rack in a 60 C oven for 30 mins prior to staining. We do not do on board baking (primarily because on board baking is only 10 mins long and with the section lifting issue we want longer). Some of the specimen types are more susceptible it would seem. Cervix LEEP specimens tend to be bad, we use a 4mm punch to obtain some of our control tissue and so the breast tissue we use for Myosin Heavy Chain seems to be a bad one and sometimes our ER/PR control sections (but not always). Fine needle cores where a lot of tumour is present tend to be bad (again not always). We have tried baking longer, we turn ourselves inside out trying to get all of the water out from under the sections, we have tried charged slides from another manufacturer. I have looked at the HIER protocols and none are extraordinary in nature. We use very clean covertiles (no scratches or blemishes). Our specimens are fixed for 24hrs before processing. We use the same slides for Special Stains and don't have this issue there. I need some new suggestions to try! All ideas welcome. Cheers, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ________________________________ Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From melissa at alliedsearchpartners.com Wed Jul 12 14:59:14 2017 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Wed, 12 Jul 2017 19:59:14 +0000 Subject: [Histonet] Permanent/Full Time Histotech Job in Panama City, FL Message-ID: Hello All, I have a full time/permanent position for a Histotech in Panama City, FL. This is a Monday-Friday Day Shift opportunity and I am looking for a long term permanent candidate. Please let me know if you are interested and I will provide more information. Thank you! Melissa Owens President, Laboratory Staffing Allied Search Partners Direct Line: 407.413.9117 Toll Free: 888.388.7571 ext. 102 F: 888.388.7572 From LRaff at uropartners.com Thu Jul 13 06:43:46 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 13 Jul 2017 11:43:46 +0000 Subject: [Histonet] New Lab Related Blog Post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF114F70C9@COLOEXCH01.uropartners.local> Good morning to all. The rains in Chicago have finally stopped. Link for a lab blog post is below. http://www.chicagonow.com/downsize-maybe/2017/07/oops-we-did-it-again-another-research-paper-tells-us-pathologists-sometimes-have-it-tough-this-time-with-melanoma/ Thanks, Lester J. Raff, MD MBA Laboratory Medical Director From KSimeone at leavittmgt.com Thu Jul 13 12:33:38 2017 From: KSimeone at leavittmgt.com (Kari Simeone) Date: Thu, 13 Jul 2017 17:33:38 +0000 Subject: [Histonet] IHC sections adherence Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CC95F600D@vm-email.leavittmgt.com> Hi Greg, I use BONDS and Apex slides as well. What we do with tissue that washes (usually heavy tumor and some harsher stains like CD34) is leave the tissue unbaked at room temp for 12 hours and then bake at 60 degrees for 30 minutes. I don't know if you are including your control tissue on the same slide (we do) but we also cut a separate slide with just patient sections and that seems to help with extra paraffin runoff from the control tissue underneath the patient tissue. We've been doing this successfully for years. I hope this is helpful, I totally understand your frustration! Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor ________________________________________ From: histonet-request at lists.utsouthwestern.edu [histonet-request at lists.utsouthwestern.edu] Sent: Thursday, July 13, 2017 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 164, Issue 9 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=kGqc4zz3kX--1rHjY-YoBfcDwuXTr_4WO7sfWADnB6s&s=gVV45__1yJfu6axbr8oxcPYBvFX1MiI_GOD2r2Kpv_g&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Section adherence issues (IHC) (Walter Benton) 2. Permanent/Full Time Histotech Job in Panama City, FL (Melissa Owens) 3. New Lab Related Blog Post (Lester Raff MD) ---------------------------------------------------------------------- Message: 1 Date: Wed, 12 Jul 2017 17:37:38 +0000 From: Walter Benton To: "Hujet, Matthew" , Greg Dobbin Cc: "kgeveleigh at ihis.org" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Section adherence issues (IHC) Message-ID: <816e042155824c4193f79c50c1461b48 at MAIL01.GCU-MD.local> Content-Type: text/plain; charset="us-ascii" Thanks. This is helpful information. -----Original Message----- From: Hujet, Matthew [mailto:Matthew.Hujet at ssmhealth.com] Sent: Wednesday, July 12, 2017 1:35 PM To: Walter Benton ; Greg Dobbin Cc: kgeveleigh at ihis.org; histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] Section adherence issues (IHC) We have had really good luck with both the TOMO and Millennia 2000 adhesion slides. We initially tried using the TOMO slides for everything (IHC, special stains, and H&E's), but found that there was too much background staining on the specials and H&E's. For IHC, the TOMO slides were great. We then switched to the Millennia 2000 for everything, and they have been working really well across the board. With regards to just IHC, I personally feel that the TOMO slides are better, but if you only want one type of glass in your lab, the Millennia 2000 are the way to go. Matthew Hujet Histology Technical Specialist SSM Health St. Mary's Hospital - Madison 700 S. Park St. Madison, WI 53715 Please note my new email address: Matthew.Hujet at ssmhealth.com -----Original Message----- From: Walter Benton [mailto:wbenton at cua.md] Sent: Wednesday, July 12, 2017 10:34 AM To: Greg Dobbin Cc: kgeveleigh at ihis.org; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Section adherence issues (IHC) Greg, We are running into a similar issue on the Biocare Nemesis platform and the support there suggest humidity to be the issue. Our sections stay adhered to the slides through depar, antigen retrieval, but appear to fall off during the staining process. It is not consistent. We are going to try some other slides and see if the issue persists. I'd be interested to hear your findings and resolution. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 -----Original Message----- From: Greg Dobbin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, July 12, 2017 11:09 AM To: histonet at lists.utsouthwestern.edu Cc: kgeveleigh at ihis.org Subject: [Histonet] Section adherence issues (IHC) Hi Folks, I haven't been on here much lately but it is nice to know that we are all here for each other when needed! My problem is section adherence during IHC staining. And it is not all of the time it is intermittent; so not all of the time and not on all slides when it does occur. Background: We have a Bond-III immunostainer and we use the Leica Apex charged slides for our IHC stains. We have no additives in our water baths. Our sections drain in a stand and then any trapped water under the section is either flicked or wicked away as needed prior to placing the slides in a rack in a 60 C oven for 30 mins prior to staining. We do not do on board baking (primarily because on board baking is only 10 mins long and with the section lifting issue we want longer). Some of the specimen types are more susceptible it would seem. Cervix LEEP specimens tend to be bad, we use a 4mm punch to obtain some of our control tissue and so the breast tissue we use for Myosin Heavy Chain seems to be a bad one and sometimes our ER/PR control sections (but not always). Fine needle cores where a lot of tumour is present tend to be bad (again not always). We have tried baking longer, we turn ourselves inside out trying to get all of the water out from under the sections, we have tried charged slides from another manufacturer. I have looked at the HIER protocols and none are extraordinary in nature. We use very clean covertiles (no scratches or blemishes). Our specimens are fixed for 24hrs before processing. We use the same slides for Special Stains and don't have this issue there. I need some new suggestions to try! All ideas welcome. Cheers, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=kGqc4zz3kX--1rHjY-YoBfcDwuXTr_4WO7sfWADnB6s&s=gVV45__1yJfu6axbr8oxcPYBvFX1MiI_GOD2r2Kpv_g&e= CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ________________________________ Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ------------------------------ Message: 2 Date: Wed, 12 Jul 2017 19:59:14 +0000 From: Melissa Owens To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Permanent/Full Time Histotech Job in Panama City, FL Message-ID: Content-Type: text/plain; charset="us-ascii" Hello All, I have a full time/permanent position for a Histotech in Panama City, FL. This is a Monday-Friday Day Shift opportunity and I am looking for a long term permanent candidate. Please let me know if you are interested and I will provide more information. Thank you! Melissa Owens President, Laboratory Staffing Allied Search Partners Direct Line: 407.413.9117 Toll Free: 888.388.7571 ext. 102 F: 888.388.7572 ------------------------------ Message: 3 Date: Thu, 13 Jul 2017 11:43:46 +0000 From: Lester Raff MD To: "Histonet (histonet at lists.utsouthwestern.edu)" Subject: [Histonet] New Lab Related Blog Post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF114F70C9 at COLOEXCH01.uropartners.local> Content-Type: text/plain; charset="us-ascii" Good morning to all. The rains in Chicago have finally stopped. Link for a lab blog post is below. https://urldefense.proofpoint.com/v2/url?u=http-3A__www.chicagonow.com_downsize-2Dmaybe_2017_07_oops-2Dwe-2Ddid-2Dit-2Dagain-2Danother-2Dresearch-2Dpaper-2Dtells-2Dus-2Dpathologists-2Dsometimes-2Dhave-2Dit-2Dtough-2Dthis-2Dtime-2Dwith-2Dmelanoma_&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=kGqc4zz3kX--1rHjY-YoBfcDwuXTr_4WO7sfWADnB6s&s=BMnYbAIiXA3i7op2GB24dtCro4-e1ZM2MHR_w-FW3gc&e= Thanks, Lester J. Raff, MD MBA Laboratory Medical Director ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=1cLLjC11YCqzLhqVtNKQcyhMt62CI6G4XYiTB8c7xDA&m=kGqc4zz3kX--1rHjY-YoBfcDwuXTr_4WO7sfWADnB6s&s=gVV45__1yJfu6axbr8oxcPYBvFX1MiI_GOD2r2Kpv_g&e= ------------------------------ End of Histonet Digest, Vol 164, Issue 9 ****************************************The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From SteveM at mcclainlab.com Thu Jul 13 13:06:27 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Thu, 13 Jul 2017 18:06:27 +0000 Subject: [Histonet] Histonet Digest, Vol 164, Issue 9 slide adhesion In-Reply-To: References: Message-ID: <40CFA409-8471-42C0-B64A-533C24CF8FD2@mcclainlab.com> Greg, It sounds like 2 or 3 problems and LEEP specimens are partially heat fixed, giving addition artifacts related to poor adhesion. 1. Short processing cycles w insufficient paraffin times or dirty xylenes can do this. Double your maintenance or try the radical by removing formalin from processor adding more time in alcohol xylene and paraffins. 2. Any water during annealing on slide warmer. Summer months w high humidity may prolong drying times. 3. 30 min baking sounds short to me-skin usually needs at least an 60-90min @90C sometimes 4H, some antibodies overnight. Extra baking does not repair or compensate for the damage from #2 in my experience. Good luck. Steve A. McClain, MD From gagnone at KGH.KARI.NET Thu Jul 13 12:53:24 2017 From: gagnone at KGH.KARI.NET (Gagnon, Eric) Date: Thu, 13 Jul 2017 17:53:24 +0000 Subject: [Histonet] Section adherence issues (IHC) Message-ID: <5F06C3AD0B27264CA20CFA986C87882E01CD84BE56@EXCHANGEPV3.KGH.ON.CA> Hi Greg, Permit me a few thoughts on factors not mentioned in your email: * Do your slides 'look' baked/is the wax melted when they are removed from the oven? * Also, is there variance in your oven temperature? * Especially vexing is the fact that control sections are lifting. Are the control sections pre-baked or at time of cutting the ER/PR patient sections? * Are the sections completely dry before covertiles are placed on top of the slides in the Leica staining rack? * Has Leica checked the instrument to rule out any instrument-related issues? Is the poor adherence occurring in one particular location within the instrument, or in various slide positions? Fortunately, special stains and routine staining rarely seem to be affected by the various pH/mechanical/detection system variables that exist in IHC. In our experience with Bond-III, areas of poor adherence tend to be longitudinally lifting/up 'the middle' of the slide and are usually resolved by leaving slides of only such problem tissues (as you mentioned) for longer times in the oven than normal. Hope this helps, Eric Gagnon MLT Kingston General Hospital Kingston, ON We have a Bond-III immunostainer and we use the Leica Apex charged slides for our IHC stains. We have no additives in our water baths. Our sections drain in a stand and then any trapped water under the section is either flicked or wicked away as needed prior to placing the slides in a rack in a 60 C oven for 30 mins prior to staining. We do not do on board baking (primarily because on board baking is only 10 mins long and with the section lifting issue we want longer). Some of the specimen types are more susceptible it would seem. Cervix LEEP specimens tend to be bad, we use a 4mm punch to obtain some of our control tissue and so the breast tissue we use for Myosin Heavy Chain seems to be a bad one and sometimes our ER/PR control sections (but not always). Fine needle cores where a lot of tumour is present tend to be bad (again not always). We have tried baking longer, we turn ourselves inside out trying to get all of the water out from under the sections, we have tried charged slides from another manufacturer. I have looked at the HIER protocols and none are extraordinary in nature. We use very clean covertiles (no scratches or blemishes). Our specimens are fixed for 24hrs before processing. We use the same slides for Special Stains and don't have this issue there. I need some new suggestions to try! All ideas welcome. Cheers, Greg From ccrowder25 at gmail.com Fri Jul 14 06:37:02 2017 From: ccrowder25 at gmail.com (Cheryl Crowder) Date: Fri, 14 Jul 2017 06:37:02 -0500 Subject: [Histonet] Mycoplasma staining In-Reply-To: References: Message-ID: I work with a researcher who has mycoplasma species cells on agar. He does not have access to a fluorescent microscope. Does anyone know of a technique using regular stain? From relia1 at earthlink.net Fri Jul 14 09:16:53 2017 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 14 Jul 2017 10:16:53 -0400 Subject: [Histonet] RELIA HOT JOB ALERT!! Histology Supevisor Needed in Atlanta!! Can You Help? Message-ID: <001701d2fcab$d82e8420$888b8c60$@earthlink.net> Hi Histonetters, How are you? I wanted to drop you a quick line to ask for help. I am currently recruiting for one of my best clients located in Atlanta, GA. We are looking for an experienced Histology Supervisor who is ASCP certified with at least 3 years of histology and 2 years of experience supervising a histology lab. My client is offering a great salary, terrific benefits, the stability of a large company and a great group of people to work with. The help I need from you is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them? If you are interested in this position please contact me ASAP at relia1 at earthlink.net , toll free at 866-607-3542 or cell/text at 407-353-5070. Remember if I hire someone you refer you will receive a referral bonus! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From badams at acadianagastro.com Fri Jul 14 12:49:17 2017 From: badams at acadianagastro.com (Brent Adams) Date: Fri, 14 Jul 2017 17:49:17 +0000 Subject: [Histonet] Histonet Digest, Vol 164, Issue 5 In-Reply-To: References: Message-ID: Hey folks, I am looking to purchase some blocks with positive H pylori tissue. I have never done this before as we always had plenty of positive tissue in the wet tissue room. Has anyone had to buy tissue? If you have, who did you find to be good to work with and how is it sold, by the block by the case by the pound ( LOL). Really could use some direction on this. I just cant keep enough acceptable positive H pylori control slides on hand and am always running out. Would like to have a 6 month supply to work from and be able to keep a head with. Thanks Brent Adams ? BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Saturday, July 8, 2017 12:00:01 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 164, Issue 5 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Pathology Band Saw (Sandra Cheasty) ---------------------------------------------------------------------- Message: 1 Date: Fri, 07 Jul 2017 19:33:36 +0000 From: Sandra Cheasty To: "Histonet (histonet at lists.utsouthwestern.edu)" Subject: [Histonet] Pathology Band Saw Message-ID: Content-Type: text/plain; CHARSET=US-ASCII Hello, Can anyone recommend a band saw for pathology? We are looking for a new band saw for use in necropsy and research. It has to be a "meat" band saw, as workshop band saws aren't compatible with wet tissue. A table-top model is preferred, and cheaper than a floor model. (Plus, the floor models all seem to come with a sausage-maker, which is pretty gross.) If you have used a table-top band saw in your pathology lab, I'd appreciate hearing from you. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet ------------------------------ End of Histonet Digest, Vol 164, Issue 5 **************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. From plucas at biopath.org Fri Jul 14 13:58:58 2017 From: plucas at biopath.org (Paula) Date: Fri, 14 Jul 2017 11:58:58 -0700 Subject: [Histonet] holes in GI tissue Message-ID: <003e01d2fcd3$40edc8f0$c2c95ad0$@biopath.org> Hello, Our pathologist is complaining about "holes" in the GI tissue. This is what he mainly reads, so it could be other types of tissues and the other pathologists don't complain about it. Anyway, he showed me an example, and I see what he is talking about. I think it comes down to microtomy, such as being too rapid when facing-in or having a dull knife. Maybe it could be another reason, but in any event, can anyone offer any suggestions on how to remedy this problem? Thanks in advance..I really apprecieate the feedback. Paula Lab Manager for BioPath Medical Group From SteveM at mcclainlab.com Fri Jul 14 15:23:09 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Fri, 14 Jul 2017 20:23:09 +0000 Subject: [Histonet] Histonet Digest, Vol 164, Issue 10 mycoplasma on agar In-Reply-To: References: Message-ID: <90100AD6-AD69-4911-9C10-9AB53E2FFCBB@mcclainlab.com> Too bad, Hoescht stain is rapid and sensitive (UV) for mycoplasma. They can try staining direct smears w difquik giemsa. Or fix agar plate in Carnoy's and slice out and process to paraffin for microtomy and staining. IHC can be done from sections if needed. These 1 micron bacteria are at the practical limits of visibility. Steve A. McClain, MD From naje1972 at yahoo.com Fri Jul 14 22:27:32 2017 From: naje1972 at yahoo.com (cynthia haynes) Date: Sat, 15 Jul 2017 03:27:32 +0000 (UTC) Subject: [Histonet] Histonet Digest, Vol 164, Issue 5 In-Reply-To: References: Message-ID: <2084162489.735422.1500089252592@mail.yahoo.com> Are you batch staining or you placing a control on every slide; if batches are used ?a good controld block will last ?6 month or more. Placing a control on every slide needing a stain will last 3 month. I will try to get you a block free of charge. Cynthia James H.T? Sent from Yahoo Mail on Android On Fri, Jul 14, 2017 at 12:55 PM, Brent Adams via Histonet wrote: Hey folks, I am looking to purchase some blocks with positive H pylori tissue. I have never done this before as we always had plenty of positive tissue in the wet tissue room. Has anyone had to buy tissue? If you have, who did you find to be good to work with and how is it sold, by the block by the case by the pound ( LOL). Really could use some direction on this. I just cant keep enough acceptable positive H pylori control slides on hand and am always running out. Would like to have a 6 month supply to work from and be able to keep a head with. Thanks Brent Adams ? BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA? 70503 tel:? (337) 269-1126 fax:? (337) 269-1476 ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Saturday, July 8, 2017 12:00:01 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 164, Issue 5 Send Histonet mailing list submissions to ? ? ? ? histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ? ? ? ? http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet or, via email, send a message with subject or body 'help' to ? ? ? ? histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at ? ? ? ? histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. Pathology Band Saw (Sandra Cheasty) ---------------------------------------------------------------------- Message: 1 Date: Fri, 07 Jul 2017 19:33:36 +0000 From: Sandra Cheasty To: "Histonet (histonet at lists.utsouthwestern.edu)" ? ? ? ? Subject: [Histonet] Pathology Band Saw Message-ID: ? ? ? ? Content-Type: text/plain; CHARSET=US-ASCII Hello, ? ? ? ? ? ? ? ? Can anyone recommend a band saw for pathology? We are looking for a new band saw for use in necropsy and research. It has to be a "meat" band saw, as workshop band saws aren't compatible with wet tissue. A table-top model is preferred, and cheaper than a floor model. (Plus, the floor models all seem to come with a sausage-maker, which is pretty gross.) ? ? ? ? ? ? ? ? If you have used a table-top band saw in your pathology lab, I'd appreciate hearing from you. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet ------------------------------ End of Histonet Digest, Vol 164, Issue 5 **************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From boznpl at aol.com Sat Jul 15 14:17:02 2017 From: boznpl at aol.com (Laurie Colbert) Date: Sat, 15 Jul 2017 15:17:02 -0400 Subject: [Histonet] Histology Supervisor Position in Los Angeles CA Message-ID: <15d47aec402-2ed9-7a03@webprd-a39.mail.aol.com> PATH MD has an immediate opening for a Histology Supervisor. PATH MD is a state of the art histology lab in West Hollywood and offers competitive salaries, full health insurance and a matching 401(k) plan.? Candidates must have prior experience and be ASCP registered.? Contact David Costanzo at (323) 648-3212 work or (213) 437-6400 cell. From john.frazier at roche.com Sat Jul 15 18:21:49 2017 From: john.frazier at roche.com (Frazier, John) Date: Sat, 15 Jul 2017 19:21:49 -0400 Subject: [Histonet] Histonet Digest, Vol 164, Issue 10 mycoplasma on agar Message-ID: <1324243570961369371@unknownmsgid> I would have the ordering physician to order a cold agglutinin on the patient. This is a a good indicator if Mycoplasma is present. Sent from my iPad > On Jul 14, 2017, at 4:23 PM, Steve McClain wrote: > > Too bad, Hoescht stain is rapid and sensitive (UV) for mycoplasma. > > They can try staining direct smears w difquik giemsa. > > Or fix agar plate in Carnoy's and slice out and process to paraffin for microtomy and staining. IHC can be done from sections if needed. > These 1 micron bacteria are at the practical limits of visibility. > > Steve A. McClain, MD > > > From ccrowder25 at gmail.com Sun Jul 16 12:15:16 2017 From: ccrowder25 at gmail.com (Cheryl Crowder) Date: Sun, 16 Jul 2017 12:15:16 -0500 Subject: [Histonet] Mycoplasma replies In-Reply-To: References: Message-ID: I want to thank those who responded to my inquiry about staining mycoplasma. The researcher has access to DiffQuik stains in particular and is going to.try that first. Wish us luck.z Cheryl Cprowder From ronald.kusters at pfmmedical.com Mon Jul 17 02:17:19 2017 From: ronald.kusters at pfmmedical.com (Kusters, Ronald) Date: Mon, 17 Jul 2017 07:17:19 +0000 Subject: [Histonet] Pathology Band Saw In-Reply-To: References: Message-ID: <0b33fcf0b6b04bdeab416ff5cf720323@EX-PFM-103-W.pfm-ag.net> Dear Sandra, You could have a look at EXAKT, a high end saw designed for Pathology. http://exaktusa.com/pathology-equipment/ Success, Ronald Mit freundlichen Gr??en/Kind Regards Ronald Kusters International Sales Manager Feather Brand Histotechnology pfm medical ag Wankelstra?e 60 50996 K?ln, Germany T +49 (2236) 9641 660 F +49 (2236) 9641 99 660 M +49 (171) 9144343 -----Original Message----- From: Sandra Cheasty [mailto:sandra.cheasty at wisc.edu] Sent: vrijdag 7 juli 2017 21:34 To: Histonet (histonet at lists.utsouthwestern.edu) Subject: [Histonet] Pathology Band Saw Hello, Can anyone recommend a band saw for pathology? We are looking for a new band saw for use in necropsy and research. It has to be a "meat" band saw, as workshop band saws aren't compatible with wet tissue. A table-top model is preferred, and cheaper than a floor model. (Plus, the floor models all seem to come with a sausage-maker, which is pretty gross.) If you have used a table-top band saw in your pathology lab, I'd appreciate hearing from you. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From Blanca.Lopez at UTSouthwestern.edu Mon Jul 17 11:30:15 2017 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Mon, 17 Jul 2017 16:30:15 +0000 Subject: [Histonet] Dark brown dots on my slides Message-ID: Dear Histonets: I was wondering if somebody can explain me the reason why I have a dark brown spots on my slides? How can I fix it? thanks Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From criley at dpspa.com Mon Jul 17 13:20:06 2017 From: criley at dpspa.com (Charles Riley) Date: Mon, 17 Jul 2017 14:20:06 -0400 Subject: [Histonet] Thermo Shandon excelsior help Message-ID: Does anyone using these machines have a problem with the alcohol levels in A1. My machine will not suction the alcohol out of the A1 container even though it is completely full. If anyone knows how to fix this problem (other than getting a service tech out to fix it) I would greatly appreciate it. We are getting a new processor on Friday of this week so our current processor is not under a service contract. Thanks in advance -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From NRich at bmhsc.org Tue Jul 18 11:07:11 2017 From: NRich at bmhsc.org (Nina J. Rich) Date: Tue, 18 Jul 2017 16:07:11 +0000 Subject: [Histonet] fte in histogy based on cpt codes Message-ID: <0e8d08c41dc5461e9ddd67ad448e61dd@bmhsc.org> Is anyone using CPT codes to calculate their FTE/variable labor for histology and cytology? For example how much time would be calculated for each 88305? In the main lab they use 20 minutes as a unit so if a procedure takes 40 minutes it would equal 2 Dir Variable labor units. If so, can you share what each code would equal In time or units. I realize it would have to be an average due to varying blocks counts for each case. Any input would greatly be appreciated. Thanks. Jean From Lacie.Algeo at providence.org Tue Jul 18 12:12:56 2017 From: Lacie.Algeo at providence.org (Algeo, Lacie A) Date: Tue, 18 Jul 2017 17:12:56 +0000 Subject: [Histonet] 2. fte in histogy based on cpt codes (Nina J. Rich) In-Reply-To: References: Message-ID: <24C4B3C167E5694887AB594C7602CE3A03DB3E4B@WN35104.or.providence.org> Hi Nina, If possible, run away from this process...fast! The NSH and the CAP have an excellent paper on staffing based on block volumes. If you would like, I can send it to you. That said, our establishment uses CPT counts for our staffing, however, it is not based on the type of CPT code. An 88309 is treated the same as an 88342 and so on and so forth. We get budgeted approximately .25 FTE hours for each fee code performed. This includes techs and lab assistants. So, if we had 200 fee codes in a day (IHC, SS, Gross and Micro etc.), we would be budgeted fifty hours, or 6.25 FTE for the day. Hope this helps. Best regards, Lacie Lacie Algeo, HTL (ASCP)MB Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Ave L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo at providence.org -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Tuesday, July 18, 2017 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 164, Issue 14 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=KoC5GYBOIefzxGAm2j6cjFf-Gz7ANghQIP9aFG9DuBs&r=8rnK6_huVxb-_CKs7Q80RgzczdadLMceeuDVFf9boow&m=2qYRaaavpV4pE33sLNZ2eOGjweFbVqpQnG8WgWgZ0yQ&s=JVvAssjyYYmpM-Krl_T9T6rX6HN--S0Fu70Yqqv7LpY&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Thermo Shandon excelsior help (Charles Riley) 2. fte in histogy based on cpt codes (Nina J. Rich) ---------------------------------------------------------------------- Message: 1 Date: Mon, 17 Jul 2017 14:20:06 -0400 From: Charles Riley To: Histo List Subject: [Histonet] Thermo Shandon excelsior help Message-ID: Content-Type: text/plain; charset="UTF-8" Does anyone using these machines have a problem with the alcohol levels in A1. My machine will not suction the alcohol out of the A1 container even though it is completely full. If anyone knows how to fix this problem (other than getting a service tech out to fix it) I would greatly appreciate it. We are getting a new processor on Friday of this week so our current processor is not under a service contract. Thanks in advance -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Message: 2 Date: Tue, 18 Jul 2017 16:07:11 +0000 From: "Nina J. Rich" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] fte in histogy based on cpt codes Message-ID: <0e8d08c41dc5461e9ddd67ad448e61dd at bmhsc.org> Content-Type: text/plain; charset="iso-8859-1" Is anyone using CPT codes to calculate their FTE/variable labor for histology and cytology? For example how much time would be calculated for each 88305? In the main lab they use 20 minutes as a unit so if a procedure takes 40 minutes it would equal 2 Dir Variable labor units. If so, can you share what each code would equal In time or units. I realize it would have to be an average due to varying blocks counts for each case. Any input would greatly be appreciated. Thanks. Jean ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=KoC5GYBOIefzxGAm2j6cjFf-Gz7ANghQIP9aFG9DuBs&r=8rnK6_huVxb-_CKs7Q80RgzczdadLMceeuDVFf9boow&m=2qYRaaavpV4pE33sLNZ2eOGjweFbVqpQnG8WgWgZ0yQ&s=JVvAssjyYYmpM-Krl_T9T6rX6HN--S0Fu70Yqqv7LpY&e= ------------------------------ End of Histonet Digest, Vol 164, Issue 14 ***************************************** ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From Kimberly at animalreferencepathology.com Tue Jul 18 13:35:01 2017 From: Kimberly at animalreferencepathology.com (Kimberly Marshall) Date: Tue, 18 Jul 2017 18:35:01 +0000 Subject: [Histonet] Pregnancy and the Histo lab Message-ID: Hello from Utah all Histo folks. I would like some input on Pregnancy and working in a Histology lab. I worked through both pregnancies and had healthy children. That being said I know much has changed in 30 years. So how are labs dealing with it now? Less time around formalin? Xylene? No pregnant ladies in the Histo lab??? There is some research out there, but would like to know how other histology Supervisors handle it. Thanks in advance Kimberly Marshall H.T. (ASCP) Kimberly Marshall H.T.(ASCP) Histology Lab Supervisor Toll Free 1-800-426-2099 Fax 801-584-5104 PO Box 17580 Salt Lake City, Utah 84107 www.animalreferencepathology.com Advancing the art and science of veterinary medicine From areichard at lmhealth.org Wed Jul 19 06:03:26 2017 From: areichard at lmhealth.org (Amanda Reichard) Date: Wed, 19 Jul 2017 11:03:26 +0000 Subject: [Histonet] Pregnancy and the Histo lab In-Reply-To: References: Message-ID: <9ab59b1768e447c2b9f2f6bbb528fd72@LMH-EXMB-2.lmhealth.org> Hi Kimberly, I was recently pregnant and stayed away from sentinel node specimens, and mounting media (Toluene can cause birth defects). I am also a supervisor so my exposure was limited anyways. I would say whatever the woman is comfortable with I would let them be around, except the two I stated above. If she wasn't comfortable with formalin or Americlear (our xylene sub) I would have found something else that she could do besides microtomy like filing, send outs, cytology etc. I think it's very important that we are as careful as we can be in regards to pregnancy in the histology lab. Amanda Amanda Reichard, HTL (ASCP)cm Histology/Cytology Supervisor Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (220) 564-4163 areichard at lmhealth.org -----Original Message----- From: Kimberly Marshall via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, July 18, 2017 2:35 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pregnancy and the Histo lab Hello from Utah all Histo folks. I would like some input on Pregnancy and working in a Histology lab. I worked through both pregnancies and had healthy children. That being said I know much has changed in 30 years. So how are labs dealing with it now? Less time around formalin? Xylene? No pregnant ladies in the Histo lab??? There is some research out there, but would like to know how other histology Supervisors handle it. Thanks in advance Kimberly Marshall H.T. (ASCP) Kimberly Marshall H.T.(ASCP) Histology Lab Supervisor Toll Free 1-800-426-2099 Fax 801-584-5104 PO Box 17580 Salt Lake City, Utah 84107 www.animalreferencepathology.com Advancing the art and science of veterinary medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 220-564-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From criley at dpspa.com Wed Jul 19 08:17:24 2017 From: criley at dpspa.com (Charles Riley) Date: Wed, 19 Jul 2017 09:17:24 -0400 Subject: [Histonet] Microtomy question Message-ID: We currently are having issues with our pathologists asking for deeper levels. They complain that they are not finding tumors that should be there and need us to go deeper. Does anyone have any suggestions on how far into a block I should explain to my techs to go in order to help them get over the fear of going too deep into a block? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From rjbuesa at yahoo.com Wed Jul 19 08:55:33 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 19 Jul 2017 13:55:33 +0000 (UTC) Subject: [Histonet] Microtomy question In-Reply-To: References: Message-ID: <540240708.438515.1500472533823@mail.yahoo.com> It is impossible to say how deep to cut into a block. First the PA must be sure s/he was sure the tumor/lesion was in the piece submitted to process. If that is the case, the one who casted the block must be sure the lesion is as near the block surface as possible in a way that when the block is trimmed, the lesion is macroscopically visible.The rest depends on the pathologist is s/he can make the diagnosis?with the slides first submitted or if deeper sections are required.Usually deeper sections are requested when the?tumor or abnormal tissue is visible and more information is required. It is very?rare?that a pathologist requests deeper to find the lesion, unless trimming has been so superficial that the slide barely reveals the processed tissue.Usually lesion will be near the center of the tissue processed because the PA leaves tissue around the lesion. If this practice is followed, the histotechs should trim until the sections closely has the same size (area) as the processed tissue.If the pathologist asks for deepers several times, and s/he is sure there?should be?a lesion in the section, keep presenting sections until the lesion is found.As an advise, do not discard sections and keep at least 1 every 5 sections to prevent a very small lesion is lost for ever and the diagnosis cannot be made.Consult always with your pathologist, especially asking if there should be a lesion, because the whole problem may reside in the fact that the PA did not include what the pathologist is looking for in the tissue submitter for processing.Ren? On Wednesday, July 19, 2017 9:30 AM, Charles Riley via Histonet wrote: We currently are having issues with our pathologists asking for deeper levels. They complain that they are not finding tumors that should be there and need us to go deeper. Does anyone have any suggestions on how far into a block I should explain to my techs to go in order to help them get over the fear of going too deep into a block? -- Charles Riley BS? HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet | | Virus-free. www.avast.com | From Kelly.Pairan at nationwidechildrens.org Wed Jul 19 12:32:20 2017 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Wed, 19 Jul 2017 17:32:20 +0000 Subject: [Histonet] Pregnancy and the Histo Lab Message-ID: <3a0e3877caf0465a8f718f5ca80de318@l1perdwmbx01.childrensroot.net> Hi Kimberly, I was pregnant last year. I pretty much did everything that I normally did in the lab. When I thought I may have a prolonged exposure to chemicals (such as changing processors), I wore a mask. The biggest issue I had was that my ankles and feet got really swollen. The histology lab is not a place where you can put your feet up and keep working. My manager did try to get me to sit with my feet up as much as possible but it was almost impossible as I do not have a desk job. I ended up being off a week before my due date because of it. My little boy just turned one and he is perfectly fine. Have a great day! Kelly From SteveM at mcclainlab.com Wed Jul 19 13:34:54 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Wed, 19 Jul 2017 18:34:54 +0000 Subject: [Histonet] Histonet Digest, Vol 164, Issue Olympus microscope camera DP70 works on Windows 10 In-Reply-To: References: Message-ID: <9950B618-F084-4C1F-9BFB-F75B1C277ECB@mcclainlab.com> Yes, to my amazed relief, Olympus microscope camera DP70 works on Windows 10. These old but reliable cameras can be upgraded from Windows 7 (Vista drivers) to Windows 10. We just upgraded one of my workstations and it works fine. Steve A. McClain, MD From Lacie.Algeo at providence.org Wed Jul 19 17:48:39 2017 From: Lacie.Algeo at providence.org (Algeo, Lacie A) Date: Wed, 19 Jul 2017 22:48:39 +0000 Subject: [Histonet] H3K27M Message-ID: <24C4B3C167E5694887AB594C7602CE3A03DB4429@WN35104.or.providence.org> Hi, Is anyone out there running K27? We are looking to bring it online, but are in a situation where we can't find positive control tissue until we have a test....and we can't develop the test until we have positive control tissue. Any thoughts, or does anyone have positive slides they would be willing to share? We are running ATRX and IDH1, and I would be willing to help anyone out with these in return. Thank you, Lacie Lacie Algeo, HTL (ASCP)MB Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Ave L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo at providence.org ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From katelin09htl at gmail.com Thu Jul 20 08:15:59 2017 From: katelin09htl at gmail.com (Katelin Tellechea) Date: Thu, 20 Jul 2017 06:15:59 -0700 Subject: [Histonet] Pregnancy and the Histo lab In-Reply-To: References: Message-ID: Hi Kimberly, I did a poster presentation on this topic at the NSH Symposium in Long Beach, 2016. There is new research that most of us aren't up to date on and I feel strongly about using it to create a new culture of safety for our pregnant histotechs. I would be happy to send you a copy of my poster and I am working with the NSH Health and Safety Committee to create a user friendly version of it for The Block. The Block also has the abstract, podcast interview, and a Safety Snippet of my poster. In the Fall 2016 Mikro-Graf newsletter (published by the Michigan Society of Histotechnologists) my poster findings are published in the lead article. Also, you can listen to my podcast interview on the NSH podcast website http://nshpodcasts.podbean.com (P-17, posted 11/7/2016). Please contact me directly if you have any other questions or I can help in any way. Katelin On Jul 18, 2017 11:56 AM, "Kimberly Marshall via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > Hello from Utah all Histo folks. > > I would like some input on Pregnancy and working in a Histology lab. I > worked through both pregnancies and had healthy children. That being said > I know much has changed in 30 years. So how are labs dealing with it now? > Less time around formalin? Xylene? No pregnant ladies in the Histo lab??? > > There is some research out there, but would like to know how other > histology Supervisors handle it. > > Thanks in advance > Kimberly Marshall H.T. (ASCP) > > > > Kimberly Marshall H.T.(ASCP) > Histology Lab Supervisor > Toll Free 1-800-426-2099 > Fax 801-584-5104 > PO Box 17580 > Salt Lake City, Utah 84107 > www.animalreferencepathology.com > > Advancing the art and science of veterinary medicine > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Heather.Seeley at tenethealth.com Thu Jul 20 12:16:31 2017 From: Heather.Seeley at tenethealth.com (Seeley, Heather) Date: Thu, 20 Jul 2017 17:16:31 +0000 Subject: [Histonet] FT Days Rock Hill, SC Message-ID: <9DFE334E776E734D9D231EF60CCE93C587919343@TENHDCTHMB10-31.tenethealth.net> Hey Everyone!! We have a position opening up and I wanted to let everyone know in case there is any interest. We all work FT M-F. There will be three other techs, so four total with the new employee. There are three pathologists, two transcriptionists, a PA, and two cytotechs and we are like family in here! We do monthly fun lunches as well as a lunch club, Secret Sister, and we even do artsy things sometimes on weekends. We are looking for someone with a positive and energetic mindset that will fit in, but also someone with good work habits and some experience. We would love to hear from you! Please contact me directly at heather.seeley at tenethealth.com for more info! HEATHER SEELEY, HT(ASCP) Histotech 803-985-4676 OFFICE 803-327-7598 FAX From eddessa at emory.edu Thu Jul 20 12:50:52 2017 From: eddessa at emory.edu (Dessasau III, Evan) Date: Thu, 20 Jul 2017 17:50:52 +0000 Subject: [Histonet] Employment opportunity Message-ID: Hi, this is an employment opportunity post. We have an entry level position open in our lab. We are a basic paraffin based lab with EM located at the Yerkes Research Center of Emory University in Atlanta, GA. Mostly Clinical, low volume, with some revenue work which may grow in the future. Please use the link to locate the position. Please contact me with questions. https://sjobs.brassring.com/1033/ASP/TG/cim_jobdetail.asp?partnerid=25066&siteid=5043&areq=72209br Thank you, E-van E-van D. Dessasau,III Supervisor, Histology Lab Yerkes NPRC of Emory University 954 Gatewood Rd. Atlanta, GA 30329 eddessa at emory.edu Lab (404) 727-7744 Office (404) 727-7902 ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From jstaruk at masshistology.com Fri Jul 21 07:43:10 2017 From: jstaruk at masshistology.com (Mass Histology) Date: Fri, 21 Jul 2017 08:43:10 -0400 Subject: [Histonet] Histopathology positions available in central Massachusetts Message-ID: <073e01d3021e$e99f6c10$bcde4430$@masshistology.com> Because of a continued increased in workload, Mass Histo is expanding our day shift and soon will be adding a second shift. We're looking for experienced grossing technicians, histologists, specimen procurement techs (with knowledge of CPT coding) and lab aids. We offer a pleasant working environment, great pay and great benefits. Look us up at www.masshistology.com Thanks _______________________ James E.?Staruk HT(ASCP) ? From hhuggins at novalabs.co Fri Jul 21 10:01:45 2017 From: hhuggins at novalabs.co (Haley Huggins) Date: Fri, 21 Jul 2017 08:01:45 -0700 Subject: [Histonet] IHC for Lupus In-Reply-To: References: Message-ID: Is there an IHC antibody that tests for Lupus? Does anyone know? If so which? From susanhay59 at gmail.com Fri Jul 21 10:18:33 2017 From: susanhay59 at gmail.com (S hay) Date: Fri, 21 Jul 2017 10:18:33 -0500 Subject: [Histonet] GI biopsy VIP tissue processor times In-Reply-To: References: Message-ID: Good morning everyone: Would any of my esteemed colleagues be willing to share their GI biopsy processor times on the Sakura VIP processors or the processor your lab uses? Looking for overall run times, reagents used, time in each reagent, processing temperature, etc. Feel free to email them to me at sjh1385 at bjc.org. Thanks, in advance. Have a super weekend! Susan From relia1 at earthlink.net Fri Jul 21 12:28:06 2017 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 21 Jul 2017 13:28:06 -0400 Subject: [Histonet] Happy Friday! TGIF and Some exciting new Histology opportunities from Pam Barker at RELIA Solutions! Message-ID: <00d301d30246$b76ce650$2646b2f0$@earthlink.net> Hi Histonetters! I hope you are looking forward to a great weekend! My phone has been ringing off the hook this week!! Are you ready to make a move? Do you know anybody who might be ready to make a move? (Refer them to me and you will earn a referral reward when I place them!) Here is a list of my BEST opportunities: Histology Lab Director - Atlanta, GA Histology Supervisor - Atlanta, GA Histotechnician - Irvine, CA Lead Histotech - Pomona, CA Histology tech - Lexington, MA Histotechnician - Modesto IHC Tech - Modesto Histology Tech - Nacogdoches, TX Grossing Histotech - San Diego Grossing Histotech- Chattanooga, TN - Histotech- Glenwood Springs, CO All of these opportunities are full time permanent positions with some of the leading employers nationwide. There are 1st, 2nd and 3rd shift positions open. Experienced and entry level and ASCP or eligible!! My clients offer excellent compensation, benefits and relocation/sign on bonuses. And they can't wait to speak to you!!! Please contact me at relia1 at earthlink.net or toll free at 866-607-3542 or call/text me on my cell at 407-353-5070 if you are interested in more information on any of these positions or if you would like for me to work on a custom job search for you. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Linda.Margraf at cookchildrens.org Fri Jul 21 16:00:22 2017 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Fri, 21 Jul 2017 21:00:22 +0000 Subject: [Histonet] FW: PAX-5 In-Reply-To: <0A8193BF-77A0-463C-AE6D-2F411FC8FC94@verizon.net> References: <0A8193BF-77A0-463C-AE6D-2F411FC8FC94@verizon.net> Message-ID: <5aaf2d88077b43fe865a9faf8dba9241@MBX10.CCHCS.LDAP> Here is a message I am posting for Beth?. From: "O'neil, Beth" > Date: July 21, 2017 at 2:31:24 PM CDT To: "histonet-owner at lists.utsouthwestern.edu" > Subject: PAX-5 My pathologists are not happy with PAX-5 on the Ventana Ultra; it will occasionally show cytoplasmic staining of the Reed-Sternberg cells (supposed to be a nuclear stain). We use Ventana?s clone SP34. I have been trying to optimize with the Optiview kit but we are getting so much background staining and only sporadic staining of the R-S cells. I tried a different clone, Cell Marque EP156 which was really clean but would not stain the R-S cells at all, no matter what we did. My Ventana technical specialist is stumped as to why we?re having so much trouble. Does anyone else have the same problem or have any suggestions? Beth Ann O?Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor, Technical Specialist Lab: 304 ? 293 ? 6014 Office: 304 ? 293 ? 7629 oneilb at wvumedicine.org ________________________________ From blayjorge at gmail.com Sat Jul 22 14:02:14 2017 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Sat, 22 Jul 2017 15:02:14 -0400 Subject: [Histonet] Blood "donations" for money Message-ID: Dear Colleagues: Close to where I leave, there is an establishment for blood "donations". Apparently, the establishment pays per donation. I hypothesize that the money explains why the place is generally "hopping" (today, ca. 8:30AM, there were ca, 25-30 cars parked in front of the establishment; Sunday mornings, same story). Regularly, I see trash out of the store (incl. blood splatter marks on the sidewalk, gauze, etc.). Can someone tell me: 1. Where can one find information of the internal operations of establishments like this? 2. Where can one report concerns about establishments like this? 3. More broadly, how can anyone *scientifically* tell whether the blood "donated" at those (or any other) establishment is "safe" for use by other humans? Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From rsrichmond at gmail.com Sun Jul 23 12:51:28 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Sun, 23 Jul 2017 13:51:28 -0400 Subject: [Histonet] Blood donations for money Message-ID: Jorge A. Santiago-Blay, PhD asks about blood donation for money. I suppose he's in the US. I don't think there's any paid donation of whole blood in the US any more. This is probably a plasmapheresis center, where people donate twice a week. The red blood cells are returned to the donor. Two cycles of this are usually done at a session. Many, though not all, plasma donors are pretty sleazy people. I'd ask the plasma center first, then complain to local authorities about it. Most of these plasma centers are franchise operations, and you could complain to their managers also. Most plasma products (derivatives) can be sterilized so they don't transmit viruses. Or so we hope. Bob Richmond Samurai Pathologist Maryville TN ****************************************** > Close to where I leave, there is an establishment for blood "donations". > Apparently, the establishment pays per donation. I hypothesize that the > money explains why the place is generally "hopping" (today, ca. 8:30AM, > there were ca, 25-30 cars parked in front of the establishment; Sunday > mornings, same story). Regularly, I see trash out of the store (incl. blood > splatter marks on the sidewalk, gauze, etc.). > > Can someone tell me: > 1. Where can one find information of the internal operations of > establishments like this? > > 2. Where can one report concerns about establishments like this? > > 3. More broadly, how can anyone *scientifically* tell whether the blood > "donated" at those (or any other) establishments is "safe" for use by other > humans? > > Jorge A. Santiago-Blay, PhD > blaypublishers.com > > From tony.henwood at health.nsw.gov.au Sun Jul 23 19:06:17 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon, 24 Jul 2017 00:06:17 +0000 Subject: [Histonet] PAX-5 In-Reply-To: <5aaf2d88077b43fe865a9faf8dba9241@MBX10.CCHCS.LDAP> References: <0A8193BF-77A0-463C-AE6D-2F411FC8FC94@verizon.net> <5aaf2d88077b43fe865a9faf8dba9241@MBX10.CCHCS.LDAP> Message-ID: <0237449DE79DBC45B686AB82CDCD16FF95561E6E@SVDCMBX-MEX008.nswhealth.net> Hi Beth, We have found the same thing. You get a moderate staining (half of what you see with most B cell lymphomas) with clone 1EW (as supplied with the Leica Bond rtu). You will get similar results with clone 24 (38%: Adams, et al (2009). Clinical, phenotypic and genetic similarities and disparities between post-transplant and classical Hodgkin lymphomas with respect to therapeutic targets. Expert opinion on therapeutic targets, 13(10), 1137-1145.) We have found that using the Rabbit monoclonal antibody to Pax-5 (Clone SP34), it seems that, though staining a similar proportion of B cell lymphomas, staining is absent from the Reed-Sternberg cells of Hodgin lymphoma. Our assessment is in its early stages so with further cases, it might be clearer. It seems that not all Pax-5 antibodies react the same Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Linda Margraf via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Saturday, 22 July 2017 7:00 AM To: histonet at lists.utsouthwestern.edu Cc: 'oneilb at wvumedicine.org' Subject: [Histonet] FW: PAX-5 Here is a message I am posting for Beth?. From: "O'neil, Beth" > Date: July 21, 2017 at 2:31:24 PM CDT To: "histonet-owner at lists.utsouthwestern.edu" > Subject: PAX-5 My pathologists are not happy with PAX-5 on the Ventana Ultra; it will occasionally show cytoplasmic staining of the Reed-Sternberg cells (supposed to be a nuclear stain). We use Ventana?s clone SP34. I have been trying to optimize with the Optiview kit but we are getting so much background staining and only sporadic staining of the R-S cells. I tried a different clone, Cell Marque EP156 which was really clean but would not stain the R-S cells at all, no matter what we did. My Ventana technical specialist is stumped as to why we?re having so much trouble. Does anyone else have the same problem or have any suggestions? Beth Ann O?Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor, Technical Specialist Lab: 304 ? 293 ? 6014 Office: 304 ? 293 ? 7629 oneilb at wvumedicine.org ________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From criley at dpspa.com Mon Jul 24 08:48:16 2017 From: criley at dpspa.com (Charles Riley) Date: Mon, 24 Jul 2017 09:48:16 -0400 Subject: [Histonet] Fungus Controls Message-ID: Does anyone grow their own fungus control blocks for GMS and AFB stains? If so would you mind sharing your procedure for doing so. I tried using rotten oranges but the sample wouldn't hold onto the counterstain. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From akemiat3377 at gmail.com Mon Jul 24 10:11:40 2017 From: akemiat3377 at gmail.com (Akemi) Date: Mon, 24 Jul 2017 08:11:40 -0700 Subject: [Histonet] Fungus Controls In-Reply-To: References: Message-ID: <6E924300-3BA1-4271-9B8A-8E3D9C754F89@gmail.com> I've been told by one of my pathologists that blue cheese for a fungus control would work. Akemi Allison Sent from my iPhone > On Jul 24, 2017, at 6:48 AM, Charles Riley via Histonet wrote: > > Does anyone grow their own fungus control blocks for GMS and AFB stains? > > If so would you mind sharing your procedure for doing so. I tried using > rotten oranges but the sample wouldn't hold onto the counterstain. > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf at tidelandshealth.org Mon Jul 24 11:39:16 2017 From: ASelf at tidelandshealth.org (Amy Self) Date: Mon, 24 Jul 2017 12:39:16 -0400 Subject: [Histonet] Lung Tumor Profile Ordering Message-ID: Question about ordering Lung Tumor Profiles; How are lung tumor profiles being ordered at your facility; at the time the pathologist is diagnosing or are they ordered at a later date by the patient's oncologist? For those of you that order at time of diagnosing are you getting reimbursed or are the charges being rejected by Medicare and a few other insurance companies that follow Medicare? Thanks in advance for your help, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From john.frazier at roche.com Mon Jul 24 12:58:13 2017 From: john.frazier at roche.com (Frazier, John) Date: Mon, 24 Jul 2017 12:58:13 -0500 Subject: [Histonet] Blood donations for money Message-ID: <3390545875306824001@unknownmsgid> Under FDA guidelines plasmapheresis donor are held to a higher standard than red cell downers when it comes to qualifications. Plasmapheresis donors are required take a physical once a year by a registered nurse. Each plasma donor center must a medical director that can only be filled by a licensed physician. They are required to be on site minimum 20 hours a month to review laboratory results donor records, physical reviews, and consult any donors that were rejected due to positive lab test results. There are many more FDA, foreign government German Health Ministry, and internal company standards that each donor center must abide by. If not they can receive 483's which is a citation, warning letters or consent decree. Each donor is required to donate a minimum of two times with negative test results before the units can be put into production. I'll plasma is tested for viral markers, total protein, AST(liver test), West Nile, parvovirus, sexually transmitted diseases and many other lab test. This lab tests are designed to not only monitor the integrity of the plasma pool but also the donor's health. Prior to the plasma being put into production it goes through a series of detergents, cooling and heating, Ultraviolet light that will kill any viral, bacterial and or fungal material. To my knowledge there has not been any diseases acquired by recipients of the pharmaceutical derivatives from plasma donors since the 1990's. These donors may be paid but that is the only way to meet the huge supply of plasma needed to make all the different plasma based products. IVIG, RhoGam, CVM immunoglobulin, Albumin, *Alpha-1 Antitrypsin* *Here is a link to the insight of plasma donations and the governing bodies* http://www.donatingplasma.org/ from my iPhone Sent from my iPhone On Jul 23, 2017, at 12:51 PM, Bob Richmond wrote: Jorge A. Santiago-Blay, PhD asks about blood donation for money. I suppose he's in the US. I don't think there's any paid donation of whole blood in the US any more. This is probably a plasmapheresis center, where people donate twice a week. The red blood cells are returned to the donor. Two cycles of this are usually done at a session. Many, though not all, plasma donors are pretty sleazy people. I'd ask the plasma center first, then complain to local authorities about it. Most of these plasma centers are franchise operations, and you could complain to their managers also. Most plasma products (derivatives) can be sterilized so they don't transmit viruses. Or so we hope. Bob Richmond Samurai Pathologist Maryville TN ****************************************** Close to where I leave, there is an establishment for blood "donations". Apparently, the establishment pays per donation. I hypothesize that the money explains why the place is generally "hopping" (today, ca. 8:30AM, there were ca, 25-30 cars parked in front of the establishment; Sunday mornings, same story). Regularly, I see trash out of the store (incl. blood splatter marks on the sidewalk, gauze, etc.). Can someone tell me: 1. Where can one find information of the internal operations of establishments like this? 2. Where can one report concerns about establishments like this? 3. More broadly, how can anyone *scientifically* tell whether the blood "donated" at those (or any other) establishments is "safe" for use by other humans? Jorge A. Santiago-Blay, PhD blaypublishers.com From liz at premierlab.com Mon Jul 24 13:05:35 2017 From: liz at premierlab.com (Elizabeth Chlipala) Date: Mon, 24 Jul 2017 12:05:35 -0600 Subject: [Histonet] Blood donations for money In-Reply-To: <3390545875306824001@unknownmsgid> References: <3390545875306824001@unknownmsgid> Message-ID: <14E2C6176416974295479C64A11CB9AE0302CED549A0@SBS2K8.premierlab.local> I would contact your local department of public health or your states governing agency for hazardous/biological waste - for example in Colorado our hazardous waste is controlled by the Colorado Department of Health. You should be able to register a complaint through them. On a personal note and no Bob I'm not calling you out. Not all plasma donors are sleazy I personally have donated over 50 units of platelets where I used to work and was not paid for any of it. I did get to watch TV or a movie for a couple hours since I donated during work hours (I would actually do paperwork when I was on the machine). I would even donate for specific patients when necessary, apparently some patients did well with my platelets. It was really fascinating, a small suburban 400 bed hospital with a very active blood bank, I learned a lot, it was a great experience overall. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Frazier, John via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, July 24, 2017 11:58 AM To: Bob Richmond Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Blood donations for money Under FDA guidelines plasmapheresis donor are held to a higher standard than red cell downers when it comes to qualifications. Plasmapheresis donors are required take a physical once a year by a registered nurse. Each plasma donor center must a medical director that can only be filled by a licensed physician. They are required to be on site minimum 20 hours a month to review laboratory results donor records, physical reviews, and consult any donors that were rejected due to positive lab test results. There are many more FDA, foreign government German Health Ministry, and internal company standards that each donor center must abide by. If not they can receive 483's which is a citation, warning letters or consent decree. Each donor is required to donate a minimum of two times with negative test results before the units can be put into production. I'll plasma is tested for viral markers, total protein, AST(liver test), West Nile, parvovirus, sexually transmitted diseases and many other lab test. This lab tests are designed to not only monitor the integrity of the plasma pool but also the donor's health. Prior to the plasma being put into production it goes through a series of detergents, cooling and heating, Ultraviolet light that will kill any viral, bacterial and or fungal material. To my knowledge there has not been any diseases acquired by recipients of the pharmaceutical derivatives from plasma donors since the 1990's. These donors may be paid but that is the only way to meet the huge supply of plasma needed to make all the different plasma based products. IVIG, RhoGam, CVM immunoglobulin, Albumin, *Alpha-1 Antitrypsin* *Here is a link to the insight of plasma donations and the governing bodies* http://www.donatingplasma.org/ from my iPhone Sent from my iPhone On Jul 23, 2017, at 12:51 PM, Bob Richmond wrote: Jorge A. Santiago-Blay, PhD asks about blood donation for money. I suppose he's in the US. I don't think there's any paid donation of whole blood in the US any more. This is probably a plasmapheresis center, where people donate twice a week. The red blood cells are returned to the donor. Two cycles of this are usually done at a session. Many, though not all, plasma donors are pretty sleazy people. I'd ask the plasma center first, then complain to local authorities about it. Most of these plasma centers are franchise operations, and you could complain to their managers also. Most plasma products (derivatives) can be sterilized so they don't transmit viruses. Or so we hope. Bob Richmond Samurai Pathologist Maryville TN ****************************************** Close to where I leave, there is an establishment for blood "donations". Apparently, the establishment pays per donation. I hypothesize that the money explains why the place is generally "hopping" (today, ca. 8:30AM, there were ca, 25-30 cars parked in front of the establishment; Sunday mornings, same story). Regularly, I see trash out of the store (incl. blood splatter marks on the sidewalk, gauze, etc.). Can someone tell me: 1. Where can one find information of the internal operations of establishments like this? 2. Where can one report concerns about establishments like this? 3. More broadly, how can anyone *scientifically* tell whether the blood "donated" at those (or any other) establishments is "safe" for use by other humans? Jorge A. Santiago-Blay, PhD blaypublishers.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ From john.frazier at roche.com Mon Jul 24 14:26:41 2017 From: john.frazier at roche.com (Frazier, John) Date: Mon, 24 Jul 2017 14:26:41 -0500 Subject: [Histonet] Blood donations for money Message-ID: <6730790356788144296@unknownmsgid> Here is a link to the Center for blood products evaluation and research (CBER) and the FDA that regulate this industry. I have consulted for this industry as a 6 sigma black belt and MT(ASCP). The level of compliance and regulatory standards that the plasma industry has to abide by puts most clinical laboratories and anatomical pathology laboratories inspections to shame (Joint Commission, ASCP) https://www.fda.gov/AboutFDA/CentersOffices/OfficeofMedicalProductsandTobacco/CBER/default.htm Sent from my iPhone On Jul 23, 2017, at 12:51 PM, Bob Richmond wrote: Jorge A. Santiago-Blay, PhD asks about blood donation for money. I suppose he's in the US. I don't think there's any paid donation of whole blood in the US any more. This is probably a plasmapheresis center, where people donate twice a week. The red blood cells are returned to the donor. Two cycles of this are usually done at a session. Many, though not all, plasma donors are pretty sleazy people. I'd ask the plasma center first, then complain to local authorities about it. Most of these plasma centers are franchise operations, and you could complain to their managers also. Most plasma products (derivatives) can be sterilized so they don't transmit viruses. Or so we hope. Bob Richmond Samurai Pathologist Maryville TN ****************************************** Close to where I leave, there is an establishment for blood "donations". Apparently, the establishment pays per donation. I hypothesize that the money explains why the place is generally "hopping" (today, ca. 8:30AM, there were ca, 25-30 cars parked in front of the establishment; Sunday mornings, same story). Regularly, I see trash out of the store (incl. blood splatter marks on the sidewalk, gauze, etc.). Can someone tell me: 1. Where can one find information of the internal operations of establishments like this? 2. Where can one report concerns about establishments like this? 3. More broadly, how can anyone *scientifically* tell whether the blood "donated" at those (or any other) establishments is "safe" for use by other humans? Jorge A. Santiago-Blay, PhD blaypublishers.com From rsrichmond at gmail.com Mon Jul 24 15:31:36 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Mon, 24 Jul 2017 16:31:36 -0400 Subject: [Histonet] Blood donations for money In-Reply-To: <14E2C6176416974295479C64A11CB9AE0302CED549A0@SBS2K8.premierlab.local> References: <3390545875306824001@unknownmsgid> <14E2C6176416974295479C64A11CB9AE0302CED549A0@SBS2K8.premierlab.local> Message-ID: Elizabeth, I certainly wasn't talking about volunteer platelet donors, a totally different, very dedicated bunch. I would continue to observe that plasma centers are located in fairly sleazy parts of town. I wish I could continue to be a donor - whole blood, because I'm a rare donor (group O, and e Fya K neg) - but I'm on lifetime warfarin for an aortic valve prosthesis. I even went and argued with the medical director of my local donor center about it, but he said no very firmly. On Mon, Jul 24, 2017 at 2:05 PM, Elizabeth Chlipala wrote: > I would contact your local department of public health or your states > governing agency for hazardous/biological waste - for example in Colorado > our hazardous waste is controlled by the Colorado Department of Health. > You should be able to register a complaint through them. > > On a personal note and no Bob I'm not calling you out. Not all plasma > donors are sleazy I personally have donated over 50 units of platelets > where I used to work and was not paid for any of it. I did get to watch TV > or a movie for a couple hours since I donated during work hours (I would > actually do paperwork when I was on the machine). I would even donate for > specific patients when necessary, apparently some patients did well with my > platelets. It was really fascinating, a small suburban 400 bed hospital > with a very active blood bank, I learned a lot, it was a great experience > overall. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz at premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: Frazier, John via Histonet [mailto:histonet at lists.utsouthwestern.edu > ] > Sent: Monday, July 24, 2017 11:58 AM > To: Bob Richmond > Cc: Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Blood donations for money > > Under FDA guidelines plasmapheresis donor are held to a higher standard > than red cell downers when it comes to qualifications. > Plasmapheresis donors are required take a physical once a year by a > registered nurse. > Each plasma donor center must a medical director that can only be filled > by a licensed physician. They are required to be on site minimum 20 hours a > month to review laboratory results donor records, physical reviews, and > consult any donors that were rejected due to positive lab test results. > There are many more FDA, foreign government German Health Ministry, and > internal company standards that each donor center must abide by. If not > they can receive 483's which is a citation, warning letters or consent > decree. > Each donor is required to donate a minimum of two times with negative test > results before the units can be put into production. I'll plasma is tested > for viral markers, total protein, AST(liver test), West Nile, parvovirus, > sexually transmitted diseases and many other lab test. This lab tests are > designed to not only monitor the integrity of the plasma pool but also the > donor's health. > Prior to the plasma being put into production it goes through a series of > detergents, cooling and heating, Ultraviolet light that will kill any > viral, bacterial and or fungal material. > To my knowledge there has not been any diseases acquired by recipients of > the pharmaceutical derivatives from plasma donors since the 1990's. > > These donors may be paid but that is the only way to meet the huge supply > of plasma needed to make all the different plasma based products. IVIG, > RhoGam, CVM immunoglobulin, Albumin, *Alpha-1 Antitrypsin* > > *Here is a link to the insight of plasma donations and the governing > bodies* > > http://www.donatingplasma.org/ > > > from my iPhone > > > > Sent from my iPhone > On Jul 23, 2017, at 12:51 PM, Bob Richmond wrote: > > Jorge A. Santiago-Blay, PhD asks about blood donation for money. I suppose > he's in the US. I don't think there's any paid donation of whole blood in > the US any more. This is probably a plasmapheresis center, where people > donate twice a week. The red blood cells are returned to the donor. Two > cycles of this are usually done at a session. > > Many, though not all, plasma donors are pretty sleazy people. I'd ask the > plasma center first, then complain to local authorities about it. Most of > these plasma centers are franchise operations, and you could complain to > their managers also. > > Most plasma products (derivatives) can be sterilized so they don't > transmit viruses. Or so we hope. > > Bob Richmond > Samurai Pathologist > Maryville TN > ****************************************** > > Close to where I leave, there is an establishment for blood "donations". > > Apparently, the establishment pays per donation. I hypothesize that the > > money explains why the place is generally "hopping" (today, ca. 8:30AM, > > there were ca, 25-30 cars parked in front of the establishment; Sunday > > mornings, same story). Regularly, I see trash out of the store (incl. blood > > splatter marks on the sidewalk, gauze, etc.). > > > Can someone tell me: > > 1. Where can one find information of the internal operations of > > establishments like this? > > > 2. Where can one report concerns about establishments like this? > > > 3. More broadly, how can anyone *scientifically* tell whether the blood > > "donated" at those (or any other) establishments is "safe" for use by other > > humans? > > > Jorge A. Santiago-Blay, PhD > > blaypublishers.com > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > This email has been scanned by the Symantec Email Security.cloud service. > For more information please visit http://www.symanteccloud.com > ______________________________________________________________________ > From rsrichmond at gmail.com Mon Jul 24 15:35:11 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Mon, 24 Jul 2017 16:35:11 -0400 Subject: [Histonet] Blood donations for money In-Reply-To: <6730790356788144296@unknownmsgid> References: <6730790356788144296@unknownmsgid> Message-ID: I had no idea that there was a shortage of immune globulin. When I was with Red Cross in the 1970s, we were awash in it, though only a few batches were usable IV, a technology that was just developing then. If we disrupt the health care payment system, the plight of our hemophiliacs will be really desperate. I helped take care of the generation of those boys that we were to lose to AIDS ten years later - I still grieve for them. On Mon, Jul 24, 2017 at 3:26 PM, Frazier, John wrote: > Here is a link to the Center for blood products evaluation and research > (CBER) and the FDA that regulate this industry. > I have consulted for this industry as a 6 sigma black belt and MT(ASCP). > The level of compliance and regulatory standards that the plasma industry > has to abide by puts most clinical laboratories and anatomical pathology > laboratories inspections to shame (Joint Commission, ASCP) > > https://www.fda.gov/AboutFDA/CentersOffices/OfficeofMedicalProductsandToba > cco/CBER/default.htm > > Sent from my iPhone > > On Jul 23, 2017, at 12:51 PM, Bob Richmond wrote: > > Jorge A. Santiago-Blay, PhD asks about blood donation for money. I suppose > he's in the US. I don't think there's any paid donation of whole blood in > the US any more. This is probably a plasmapheresis center, where people > donate twice a week. The red blood cells are returned to the donor. Two > cycles of this are usually done at a session. > > Many, though not all, plasma donors are pretty sleazy people. I'd ask the > plasma center first, then complain to local authorities about it. Most of > these plasma centers are franchise operations, and you could complain to > their managers also. > > Most plasma products (derivatives) can be sterilized so they don't transmit > viruses. Or so we hope. > > Bob Richmond > Samurai Pathologist > Maryville TN > ****************************************** > > Close to where I leave, there is an establishment for blood "donations". > > Apparently, the establishment pays per donation. I hypothesize that the > > money explains why the place is generally "hopping" (today, ca. 8:30AM, > > there were ca, 25-30 cars parked in front of the establishment; Sunday > > mornings, same story). Regularly, I see trash out of the store (incl. blood > > splatter marks on the sidewalk, gauze, etc.). > > > Can someone tell me: > > 1. Where can one find information of the internal operations of > > establishments like this? > > > 2. Where can one report concerns about establishments like this? > > > 3. More broadly, how can anyone *scientifically* tell whether the blood > > "donated" at those (or any other) establishments is "safe" for use by other > > humans? > > > Jorge A. Santiago-Blay, PhD > > blaypublishers.com > > > > > From GauchV at mail.amc.edu Tue Jul 25 08:24:59 2017 From: GauchV at mail.amc.edu (Gauch, Vicki) Date: Tue, 25 Jul 2017 13:24:59 +0000 Subject: [Histonet] IHC questions Message-ID: Hi all, I have been asked to post several questions regarding IHC stain issues and am hoping someone has some ideas... We are having issues with IHC cases floating (particularly breast IHC) lately and are wondering.... 1) How are people storing IHC control slides? Are they being precut and stored or cut at the time of the stain request? 2) If storing controls- are you doing anything special ? 3) How long are the IHC slides being air dried before placing in the oven ? How long are they in the oven prior to staining? This has not been such an issue in the past but recently we are experiencing it more frequently. We are looking at various things but if anyone has any suggestions, they would be greatly appreciated !!! Thanks, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From relia1 at earthlink.net Tue Jul 25 09:53:48 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 25 Jul 2017 10:53:48 -0400 Subject: [Histonet] Make the Cut! ICYMI here's my first column - Salary Negotiations! 7-25-2017 Message-ID: <0a8a01d30555$d262b680$77282380$@earthlink.net> Hi Histonetters, ICYMI - In case you missed it!! I am so excited!! My first article has been published in the NSH Quarterly Career Newsletter under my new byline: Make the Cut I will be writing for the NSH Career newsletter on a quarterly basis and a few months ago I asked for topics of interest in anticipation of this column. Thanks again for the suggestions and please keep them coming!! The first column is on Salary Negotiations. Here is the link: http://nsh.org/content/career-center-newsletter If you have a minute to read it I would love to hear what you think. I also have some exciting job opportunities to pass along. Please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral reward! I LOVE TO GIVE REFERRAL REWARDS!! SOME of these are RELIA exclusives. MOST of these offer Sign- on Bonuses and/or Relocation Assistance ALL of these Companies offer excellent compensation, benefits and great environments, opportunity for growth and great people to work with. AND THEY ALL ARE READY TO HIRE!! What more could you ask for? If You Or Anyone You Know Might Be Interested In Any A New Opportunity Please Contact Me. You can reach me by email at relia1 at earthlink.net Toll free at 866-607-3542 or on my cell at 407-353-5070. Here are some of our Hottest Opportunities: We have leadership opportunities in: Georgia and California We have Histotechnician/Histotechnologist opportunities in: California - Statewide ASCP or elig. experienced & entry level All Shifts! Texas - Dayshift Hospital environment Massachusetts - Exciting opportunities on multiple shifts! Colorado - Days Hospital environment Tennessee - Grossing histotech And my phone is still ringing off the hook so if you don't see the location you want yet drop me a line so I can let you know when something DOES come up. I could be talking to a client about your next opportunity RIGHT NOW!! Have a great day. I look forward to hearing back from you. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From cafechague at yahoo.com Tue Jul 25 09:54:45 2017 From: cafechague at yahoo.com (cafechague) Date: Tue, 25 Jul 2017 07:54:45 -0700 Subject: [Histonet] dako omnis ihc stainer pros and cons Message-ID: <481339.66749.bm@smtp209.mail.ne1.yahoo.com> hi all!we purchased a dako omnis and i would like to get some input on deparaffinization online and offline, as well as counterstaining online. i hear theres a lot of background stain when deparaffinizing online, and using the dako mayer heme stains too light. is this true?our ihc daily slide counts aren't much, averaging 150 to 200 slides a day with a 24 hour TAT. ?i did not want to batch slides since our counts are low, and I originally would like everything done on the stainer but im not sure how long the runs take having everything done online.?I'm just trying to figure out my workflow process.any comments will help! thanks,cae Sent from my Verizon, Samsung Galaxy smartphone From TNMayer at mdanderson.org Tue Jul 25 10:05:54 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Tue, 25 Jul 2017 15:05:54 +0000 Subject: [Histonet] Blood donations for money Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8839F74634@D1PWPEXMBX08.mdanderson.edu> Well I 'sold' my plasma in the early 1990's. I was in college and needed the money for gas and such. When I went to donate my blood, I was suspected of having Hep C, so I stopped selling my plasma and retested fine. The instruments looked clean, and there was no debris in the parking lot. But this was a college town area, so it was pretty busy. Not sure what happened to the place, but it was a big help when my funds were low. Toysha Message: 1 Date: Sun, 23 Jul 2017 13:51:28 -0400 From: Bob Richmond To: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Blood donations for money Message-ID: Content-Type: text/plain; charset="UTF-8" Jorge A. Santiago-Blay, PhD asks about blood donation for money. I suppose he's in the US. I don't think there's any paid donation of whole blood in the US any more. This is probably a plasmapheresis center, where people donate twice a week. The red blood cells are returned to the donor. Two cycles of this are usually done at a session. Many, though not all, plasma donors are pretty sleazy people. I'd ask the plasma center first, then complain to local authorities about it. Most of these plasma centers are franchise operations, and you could complain to their managers also. Most plasma products (derivatives) can be sterilized so they don't transmit viruses. Or so we hope. Bob Richmond Samurai Pathologist Maryville TN ****************************************** > Close to where I leave, there is an establishment for blood "donations". > Apparently, the establishment pays per donation. I hypothesize that > the money explains why the place is generally "hopping" (today, ca. > 8:30AM, there were ca, 25-30 cars parked in front of the > establishment; Sunday mornings, same story). Regularly, I see trash > out of the store (incl. blood splatter marks on the sidewalk, gauze, etc.). > > Can someone tell me: > 1. Where can one find information of the internal operations of > establishments like this? > > 2. Where can one report concerns about establishments like this? > > 3. More broadly, how can anyone *scientifically* tell whether the > blood "donated" at those (or any other) establishments is "safe" for > use by other humans? > > Jorge A. Santiago-Blay, PhD > blaypublishers.com > > The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From TNMayer at mdanderson.org Tue Jul 25 10:16:45 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Tue, 25 Jul 2017 15:16:45 +0000 Subject: [Histonet] Fungus controls Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8839F746C6@D1PWPEXMBX08.mdanderson.edu> We got some organisms from the microbiology lab and some fresh lung from autopsy. When the organisms were ready, we 'stuck' the lung with the inoculating loop and let them incubate for a few days. Then we grossed and processed the lung. We were given separate specimens for GMs, AFB, and gram. It worked great. If you don't have lung, some liver from the grocery store would work. We let that sit in the refrigerator for about two weeks, then did touch preps to see if anything was growing. It had fungus galore in the touch prep. We used that to teach students how to stain touch preps with specials. Toysha Message: 3 Date: Mon, 24 Jul 2017 09:48:16 -0400 From: Charles Riley To: Histo List Subject: [Histonet] Fungus Controls Message-ID: Content-Type: text/plain; charset="UTF-8" Does anyone grow their own fungus control blocks for GMS and AFB stains? If so would you mind sharing your procedure for doing so. I tried using rotten oranges but the sample wouldn't hold onto the counterstain. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Message: 4 Date: Mon, 24 Jul 2017 08:11:40 -0700 From: Akemi To: Charles Riley , Histonet Subject: Re: [Histonet] Fungus Controls Message-ID: <6E924300-3BA1-4271-9B8A-8E3D9C754F89 at gmail.com> Content-Type: text/plain; charset=us-ascii I've been told by one of my pathologists that blue cheese for a fungus control would work. Akemi Allison Sent from my iPhone > On Jul 24, 2017, at 6:48 AM, Charles Riley via Histonet wrote: > > Does anyone grow their own fungus control blocks for GMS and AFB stains? > > If so would you mind sharing your procedure for doing so. I tried > using rotten oranges but the sample wouldn't hold onto the counterstain. > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From eddessa at emory.edu Tue Jul 25 11:07:25 2017 From: eddessa at emory.edu (Dessasau III, Evan) Date: Tue, 25 Jul 2017 16:07:25 +0000 Subject: [Histonet] Fungus controls In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC8839F746C6@D1PWPEXMBX08.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC8839F746C6@D1PWPEXMBX08.mdanderson.edu> Message-ID: Hi Toysha, How long do you let the tissue/microbes incubate and at room temp? When we tried it in the incubator at different times the tissue was too far gone to make it useful. After a few day you still had viable tissue structure? I'm going to try the liver prep. Thank you so much if you are able to help!! E-van -----Original Message----- From: Mayer,Toysha N via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, July 25, 2017 11:17 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Fungus controls We got some organisms from the microbiology lab and some fresh lung from autopsy. When the organisms were ready, we 'stuck' the lung with the inoculating loop and let them incubate for a few days. Then we grossed and processed the lung. We were given separate specimens for GMs, AFB, and gram. It worked great. If you don't have lung, some liver from the grocery store would work. We let that sit in the refrigerator for about two weeks, then did touch preps to see if anything was growing. It had fungus galore in the touch prep. We used that to teach students how to stain touch preps with specials. Toysha Message: 3 Date: Mon, 24 Jul 2017 09:48:16 -0400 From: Charles Riley To: Histo List Subject: [Histonet] Fungus Controls Message-ID: Content-Type: text/plain; charset="UTF-8" Does anyone grow their own fungus control blocks for GMS and AFB stains? If so would you mind sharing your procedure for doing so. I tried using rotten oranges but the sample wouldn't hold onto the counterstain. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Message: 4 Date: Mon, 24 Jul 2017 08:11:40 -0700 From: Akemi To: Charles Riley ,Histonet Subject: Re: [Histonet] Fungus Controls Message-ID: <6E924300-3BA1-4271-9B8A-8E3D9C754F89 at gmail.com> Content-Type: text/plain;charset=us-ascii I've been told by one of my pathologists that blue cheese for a fungus control would work. Akemi Allison Sent from my iPhone > On Jul 24, 2017, at 6:48 AM, Charles Riley via Histonet wrote: > > Does anyone grow their own fungus control blocks for GMS and AFB stains? > > If so would you mind sharing your procedure for doing so. I tried > using rotten oranges but the sample wouldn't hold onto the counterstain. > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From victor_tobias at comcast.net Tue Jul 25 11:34:07 2017 From: victor_tobias at comcast.net (victor_tobias at comcast.net) Date: Tue, 25 Jul 2017 09:34:07 -0700 Subject: [Histonet] Make the Cut! ICYMI here's my first column - SalaryNegotiations! 7-25-2017 In-Reply-To: <0a8a01d30555$d262b680$77282380$@earthlink.net> References: <0a8a01d30555$d262b680$77282380$@earthlink.net> Message-ID: Pam, Nice article!! Victor Sent from Mail for Windows 10 From: Pam Barker via Histonet Sent: Tuesday, July 25, 2017 7:54 AM To: Histonetters Histonet Subject: [Histonet] Make the Cut! ICYMI here's my first column - SalaryNegotiations! 7-25-2017 Hi Histonetters, ICYMI - In case you missed it!! I am so excited!! My first article has been published in the NSH Quarterly Career Newsletter under my new byline: Make the Cut I will be writing for the NSH Career newsletter on a quarterly basis and a few months ago I asked for topics of interest in anticipation of this column. Thanks again for the suggestions and please keep them coming!! The first column is on Salary Negotiations. Here is the link: http://nsh.org/content/career-center-newsletter If you have a minute to read it I would love to hear what you think. I also have some exciting job opportunities to pass along. Please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral reward! I LOVE TO GIVE REFERRAL REWARDS!! SOME of these are RELIA exclusives. MOST of these offer Sign- on Bonuses and/or Relocation Assistance ALL of these Companies offer excellent compensation, benefits and great environments, opportunity for growth and great people to work with. AND THEY ALL ARE READY TO HIRE!! What more could you ask for? If You Or Anyone You Know Might Be Interested In Any A New Opportunity Please Contact Me. You can reach me by email at relia1 at earthlink.net Toll free at 866-607-3542 or on my cell at 407-353-5070. Here are some of our Hottest Opportunities: We have leadership opportunities in: Georgia and California We have Histotechnician/Histotechnologist opportunities in: California - Statewide ASCP or elig. experienced & entry level All Shifts! Texas - Dayshift Hospital environment Massachusetts - Exciting opportunities on multiple shifts! Colorado - Days Hospital environment Tennessee - Grossing histotech And my phone is still ringing off the hook so if you don't see the location you want yet drop me a line so I can let you know when something DOES come up. I could be talking to a client about your next opportunity RIGHT NOW!! Have a great day. I look forward to hearing back from you. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From elaineahoffman55 at yahoo.com Tue Jul 25 15:24:31 2017 From: elaineahoffman55 at yahoo.com (Elaine allison Hoffman) Date: Tue, 25 Jul 2017 20:24:31 +0000 (UTC) Subject: [Histonet] Authorization forms References: <271342043.690954.1501014271840.ref@mail.yahoo.com> Message-ID: <271342043.690954.1501014271840@mail.yahoo.com> Hi Everyone, I'm looking for some help in creating an authorization form for patients who want to pickup their own slides to take with themto their second opinion doctor or surgeon appointment.? I don't know all the legal jargon to use but I want to type up a form for the patient to sign that gives authorization and permission to give them their slides to take with them.??If anyone has such a formthat they would be willing to share, it would be greatly appreciated.? I'm just looking for some ideas how to put this form together, what it should include, and what it should look like. Thanks in advance for your help! Elaine Hoffman The Gastroenterology Clinic & Endoscopy Center, IncGI Pathology Laboratory? From tony.henwood at health.nsw.gov.au Tue Jul 25 19:22:50 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 26 Jul 2017 00:22:50 +0000 Subject: [Histonet] IHC questions In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF95562710@SVDCMBX-MEX008.nswhealth.net> Hi Vicki, We recently did a study that we published in our local journal (Histograph June 2017) that migh be of use: Controlled Section Baking for Immunohistochemistry Tony Henwood, Principal Scientist, Histopathology, The Children's Hospital at Westmead One source of poor immunostaining is overheating of tissue and sections. Several authors have reported that heated slide drying adversely affects sensitivity in immunohistochemistry (1). Therefore, some have advocated the use of lower temperature drying using adhesive-coated slides to improve the sensitivity of the test (2-5). In one study (1), half the antigens were adversely affected by section drying at 80oC including 5D3, CMV, S100, HMB45 and CEA. Oates (4) used antisera to epithelial membrane antigen from three different companies and found that for slides dried at 58"C, staining was often paler than slides dried at room temperature or at 37?C. Low heat attachment of sections to slides can cause several issues including inadequate attachment of the tissue sections so that tissue sections may be lost during antigen recovery and/or immunostaining, the inability of some paraffins to melt well at 58oC, and the requirement of more than 1 hr before an immunohistochemical procedure may be started. It has been recommended that the most efficient protocol for mounting tissue sections to microscopic slides would be to attach the tissues overnight before applying the immunohistochemical procedure at a temperature at which all tissue mounting paraffins should melt (e.g., 65oC) (6). It should be remembered that a significant dewaxing of sections occurs when slides are heated a few degrees above the melting point of the wax. Laboratory ovens seem to be variable in their ability to maintain a constant temperature with the implication that it is possible to either over-cook sections thus adversely affecting antigens or under-heat them, possibly compromising subsequent de-waxing. There is also the human element. How often are slides removed from the oven at the required time? The modern automatic immunostainers have excellent on-board slide heating in order to achieve reproducible, accurate antigen retrieval. This feature also allows controlled "baking" of sections and being able to programme a set time, removes the possibility of human error. At the Children's Hospital, the Bond 3 is used for automated immunohistochemistry. A study was designed to assess the usefulness of on-board baking in routine immunohistochemistry. Control sections were immunostained for several antigens (see table) using the Bond 3 on-board baking and dewax facility. Freshly cut sections were dried at 37oC for 5 minutes to remove excess water. Slides were then loaded onto the Bond and the baking procedure used was 35 minutes at 63oC. Stained controls were compared with control slides stained prior to the instigation of the on-board bake procedure. The historic procedure involved heating sections at 63-65oC for 35 minutes in a large fan-forced dry-air oven (7). BCL-2 Mum-1 CD31 BCL-6 Calretinin SATB2 BOB-1 S100 CyclinD1 CD20 ALK-1 MPO CD21 Ki67 INI-1 CD3 Synaptophsin BRG-1 HMB-45 Chromogranin Inhibin Melan A Myogenin Desmin The results showed that there was no difference between controls stained with the historic compared to the on-board baking procedure except for BCL-6 which the new procedure gave stronger staining. (see figure). In conclusion, we expect that on-board baking of sections should allow laboratories to have better control over the pre-analytical variables that can adversely affect the immunohistochemistry staining. References 1. Henwood, A. F. (2005). Effect of slide drying at 80oC on immunohistochemistry. Journal of Histotechnology, 28(1), 45-46. 2. Wakins, J., Kellock, D., Gillet, C., Egan, M., Pontin, J. E., Millis, R. R., & Levinson, D. A. (1990). Enhancement of immunostaining. Histopathology, 17(2), 185-185. 3. Dodson, A., Davies, E., & Waring, J. (1991). APTES, a section adhesive for immunocytochemistry; and experiences of slide drying at room temperature. Histopathology, 19(5), 484-485. 4. Oates J. (1993) The effect of temperature on immunostaining. Br J Biomed Sci 50: 157-158, 5. Williams, J. H., Mepham, B. L., & Wright, D. H. (1997). Tissue preparation for immunocytochemistry. Journal of clinical pathology, 50(5), 422-428. 6. Jones, W. T., Stockard, C. R., & Grizzle, W. E. (2001). Effects of time and temperature during attachment of sections to microscope slides on immunohistochemical detection of antigens. Biotechnic & Histochemistry, 76(2), 55-58. 7. Henwood, A. F. (2012). The application of heated detergent dewaxing and rehydration to immunohistochemistry. Biotechnic & Histochemistry, 87(1), 46-50. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Gauch, Vicki via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, 25 July 2017 11:25 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC questions Hi all, I have been asked to post several questions regarding IHC stain issues and am hoping someone has some ideas... We are having issues with IHC cases floating (particularly breast IHC) lately and are wondering.... 1) How are people storing IHC control slides? Are they being precut and stored or cut at the time of the stain request? 2) If storing controls- are you doing anything special ? 3) How long are the IHC slides being air dried before placing in the oven ? How long are they in the oven prior to staining? This has not been such an issue in the past but recently we are experiencing it more frequently. We are looking at various things but if anyone has any suggestions, they would be greatly appreciated !!! Thanks, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From esarricks at gmail.com Wed Jul 26 11:16:48 2017 From: esarricks at gmail.com (Erin Sarricks) Date: Wed, 26 Jul 2017 12:16:48 -0400 Subject: [Histonet] Searching for Horizontal Slide Racks Message-ID: Hi Histonet, I reached out about this request a few years ago with great success so I thought I would try here again. Does anyone have any slide racks that hold slides horizontally that they no longer need? I am willing to purchase and pay for shipping! I am looking for ones similar to the first few photos seen here from Ted Pella: metal microscope slide racks Thanks in advance for your help! Regards, Erin From relia1 at earthlink.net Wed Jul 26 14:09:17 2017 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 26 Jul 2017 15:09:17 -0400 Subject: [Histonet] RELIA Hot Histology Job Alert /-26-2017 Management opportunities in Dermpath in Atlanta! Message-ID: <00c801d30642$adfa5a30$09ef0e90$@earthlink.net> Hi Histonetters! Happy Hump Day! I have exciting opportunities I would like to share! I am currently working with an established growing client that is in need of a histology lab manager and a histology lab supervisor to respond to their rapid growth. My client is looking for candidates with dermpath histology experience and dermpath histology supervisory experience. This is a beautiful state of the art lab located in Atlanta, GA. My client offers a competitive salary, great benefits, relocation assistance and a fantastic team of people to work with. If you would be interested in hearing more about it please contact me. I can be reached at relia1 at earthlink.net toll free at 866-607-3542 or on my cell at 407-353-5070. Please feel free to pass along this info. If I place someone you refer you will earn a referral fee. Hope to hear from you soon.! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From relia1 at earthlink.net Fri Jul 28 09:59:51 2017 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 28 Jul 2017 10:59:51 -0400 Subject: [Histonet] RELIA Special Bulletin for Managers and Supervisors. Exciting opportuities Nationwide!! CA, GA, MA 7-28-2017 TGIF!! Message-ID: <001101d307b2$2a900110$7fb00330$@earthlink.net> Hello Histonetters, I hope you are having a great day! It?s Friday how could it not be a great day after all the worst Friday is better than the best Monday right?? I have several exciting opportunities for experienced Managers, Supervisors and Lead Techs in hospital and private lab environments in several locations throughout the US. These are some of the premier employers in the United States. The positions are of course full time and permanent. My clients offer excellent compensation, benefits and relocation assistance.? Here are my leadership positions: Boston, MA - Histology Lab Manager Atlanta, GA - Histology Lab Manager Irvine, CA ? Histology Lab Manager Atlanta, GA - Histology Supervisor Modesto, CA - Histology Supervisor Modesto, CA - Lead Histotech Ventura, CA - Lead Histotech Pomona, CA - Lead Histotech I anticipate multiple management openings with many exciting opportunities for talented managers and supervisor to be coming available nationwide as many of my clients are experiencing rapid growth again. If you are interested in looking into making a job change please let me know. That way I can keep you apprised of management opportunities that would interest you. Also if you would like more information or know of someone else who might be interested, please contact me at relia1 at earthlink.net or 866-607-3542 or on my cell at 407-353-5070. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam There are a lot of recruiters out there right now trying to work with histology professionals and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 15 years I have dedicated my practice solely to placing histology professionals like you. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Blanca.Lopez at UTSouthwestern.edu Fri Jul 28 11:51:03 2017 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Fri, 28 Jul 2017 16:51:03 +0000 Subject: [Histonet] slides/cassettes printers points of view Message-ID: Hello Histonettes!!! I was wondering if somebody are using PRIMERA cassettes and slides printers and/or SLIDEMATE slide printer and PRINTMATE AS cassette printer From Thermo. I am undecided which one to buy. I really love both. They have a lot of similarities in efficiency, price and quality BUT my manager is being hearing that primera works with cut corner slides ONLY otherwise will create a lot of problems. Have not hear too much about slidemate yet. If anybody can share their experiences with those printers that can help me to decide, I will appreciate. :) ________________________________ UT Southwestern Medical Center The future of medicine, today. From sbaldwin at mhhcc.org Fri Jul 28 13:01:23 2017 From: sbaldwin at mhhcc.org (Baldwin, Kathy) Date: Fri, 28 Jul 2017 18:01:23 +0000 Subject: [Histonet] Histologist grossing and review of cases by pathologists Message-ID: Het all I was wondering if your policy reflects what mine does because of State regulations If a Histologist is grossing with an Associate degree state wants my pathologist to review the grosses within 24 hours and we are not open Saturdays or holidays. So we created a sheet for the Histotech to put all cases she grosses on that sheet and then have them typed up and reviewed by the pathologist before he leaves for the day!! This is cumbersome for them are there any other suggestions out there?? S Kathy Baldwin ASCP, SCT Histology and Cytology Supervisor Ph 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From wbenton at cua.md Fri Jul 28 13:23:26 2017 From: wbenton at cua.md (Walter Benton) Date: Fri, 28 Jul 2017 18:23:26 +0000 Subject: [Histonet] slides/cassettes printers points of view In-Reply-To: References: Message-ID: We have the original Slidemates (not the newer AS model) and Printmate. Both instruments work well. The slidemate used a thermal ribbons to print. Certain slide coatings (in the area where you write/etch/print) are better suited for thermal print technology. Not sure what type of slides you use now, but you consider that in your purchase decision. In addition slidemates have a small footprint. Printmate also has a thermal ribbon and you can print 2D barcodes and other pertinent identifiers on the cassettes. Generally speaking the printer works well with minimal down time due to failures. Feel free to reach out if you additional questions. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 410-768-5961 (Lab) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, July 28, 2017 12:51 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] slides/cassettes printers points of view Hello Histonettes!!! I was wondering if somebody are using PRIMERA cassettes and slides printers and/or SLIDEMATE slide printer and PRINTMATE AS cassette printer From Thermo. I am undecided which one to buy. I really love both. They have a lot of similarities in efficiency, price and quality BUT my manager is being hearing that primera works with cut corner slides ONLY otherwise will create a lot of problems. Have not hear too much about slidemate yet. If anybody can share their experiences with those printers that can help me to decide, I will appreciate. :) ________________________________ UT Southwestern Medical Center The future of medicine, today. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From mkent at dermpathlab.com Fri Jul 28 13:25:01 2017 From: mkent at dermpathlab.com (Michael Kent) Date: Fri, 28 Jul 2017 14:25:01 -0400 Subject: [Histonet] UV light for von Kossa Message-ID: <2c562a902723b33a4e73859c01ab3b30@mail.gmail.com> Hi Histonet, I am looking for a cost-effective UV light for von Kossa staining. The clouds are getting tin the way. Thank you, and have a god weekend. Mike From TNMayer at mdanderson.org Mon Jul 31 09:06:04 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Mon, 31 Jul 2017 14:06:04 +0000 Subject: [Histonet] UV light for von Kossa Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8839F7E46F@D1PWPEXMBX08.mdanderson.edu> Michael, We just use one of the small desk lamps at a cutting station, the back of the embedder, and foil to reflect. It works great. Just place the coplin jar with the slides on top of the control panel of the embedder. Adjust the lamp to the top of the jar and wrap it in foil. The heat penetrates and is reflected. Voila, a von Kossa. Toysha Message: 3 Date: Fri, 28 Jul 2017 14:25:01 -0400 From: Michael Kent To: histonet at lists.utsouthwestern.edu Subject: [Histonet] UV light for von Kossa Message-ID: <2c562a902723b33a4e73859c01ab3b30 at mail.gmail.com> Content-Type: text/plain; charset="UTF-8" Hi Histonet, I am looking for a cost-effective UV light for von Kossa staining. The clouds are getting tin the way. Thank you, and have a god weekend. Mike ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 164, Issue 25 ***************************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From relia1 at earthlink.net Mon Jul 31 09:35:32 2017 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 31 Jul 2017 10:35:32 -0400 Subject: [Histonet] Can you help? Message-ID: <107901d30a0a$43c5e5f0$cb51b1d0$@earthlink.net> Hi Histonetters, I hope this is the beginning of a fantastic week for you. I am writing to you because I am hoping you can help. I have been engaged on a search by a client that is in need of a Cyto Tech in Clearwater, FL. This is a full time permanent M-F Day shift position. My client offers excellent compensation great benefits relocation assistance and a great group of people to work with. The help I need is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them? If you know anyone who might be interested in hearing more about this opportunity Please shoot me back an email with a current copy of your resume along with a good time to call you to relia1 at earthlink.net or call me on my cell at 407-353-5070. I can also be reached toll free at 866-607-3542. Remember if you refer someone to me and I place them you will earn a referral fee! Thanks-Pam Have a GreatWeek! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From mills at 3scan.com Mon Jul 31 12:55:02 2017 From: mills at 3scan.com (Caroline Miller) Date: Mon, 31 Jul 2017 10:55:02 -0700 Subject: [Histonet] UV light for von Kossa In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC8839F7E46F@D1PWPEXMBX08.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC8839F7E46F@D1PWPEXMBX08.mdanderson.edu> Message-ID: Yes, I have used desk lamps / foil and also the window on a sunny day! (caveat - mine was all research and I am unsure of the Clia rules in this space) yours, mills On Mon, Jul 31, 2017 at 7:06 AM, Mayer,Toysha N via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Michael, > > We just use one of the small desk lamps at a cutting station, the back of > the embedder, and foil to reflect. It works great. Just place the coplin > jar with the slides on top of the control panel of the embedder. Adjust > the lamp to the top of the jar and wrap it in foil. The heat penetrates > and is reflected. Voila, a von Kossa. > Toysha > > > > Message: 3 > Date: Fri, 28 Jul 2017 14:25:01 -0400 > From: Michael Kent > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] UV light for von Kossa > Message-ID: <2c562a902723b33a4e73859c01ab3b30 at mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hi Histonet, > > I am looking for a cost-effective UV light for von Kossa staining. The > clouds are getting tin the way. > > Thank you, and have a god weekend. > > Mike > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 164, Issue 25 > ***************************************** > The information contained in this e-mail message may be privileged, > confidential, and/or protected from disclosure. This e-mail message may > contain protected health information (PHI); dissemination of PHI should > comply with applicable federal and state laws. If you are not the intended > recipient, or an authorized representative of the intended recipient, any > further review, disclosure, use, dissemination, distribution, or copying of > this message or any attachment (or the information contained therein) is > strictly prohibited. If you think that you have received this e-mail > message in error, please notify the sender by return e-mail and delete all > references to it and its contents from your systems. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297