[Histonet] Questions RE: "what I have found about p16" and cellblocks for controls

[Gudrun Lang]

Your concerns are reasonable. Cyto-specimens are usually fixed in alcoholic
solutions not in NBF. Alcohol-fixation gives false positives in Her2. This
can also be seen in underfixed tissue, that is mainly fixed by the ethanols
in the processor.

Her2-protein is a normal protein on the cellsurface. FFPE treatment "turns
it down" and normal amount cannot be detected. This is "negative". Only Her2
positives have abundant protein to be detected with our methods. That is why
correct standardizised treatment is important to avoid "false positives" and
"false negatives".

Gudrun
leading histotechnologist,
Kepler Universitätsklinikum Linz
Austria

-----Ursprüngliche Nachricht-----
Von: Cassie P. Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu]

Gesendet: Freitag, 6. Januar 2017 18:26
An: histonet at lists.utsouthwestern.edu
Betreff: [Histonet] Questions RE: "what I have found about p16" and
cellblocks for controls



Is there anyone in Histonet land who is using both the Optiview and
Ultraview on the XT and ULTRA at the same time? I'd like to ask a few
questions, please email me.



We have a cytology cellblock case that came out stunningly positive for Her2
and one of our pathologist thought it would make a dynamic control if there
were any specimen left to make the control block.

While I am excited about the possiblity (Her2 control is hard to come by
around here) some concern is expressed about using it because it is initally
fixed in cytolyt before FFPE. I can argue both sides of this. I would enjoy
your input on this folks.


Cassandra Davis
Histology Technician
AP Laboratory
302-575-8095
Email:  CDavis at che-east.org<mailto:CDavis at che-east.org>


________________________________
From: Cassie P. Davis
Sent: Thursday, January 05, 2017 10:02 AM
To: histonet at lists.utsouthwestern.edu
Subject: what I have found about p16


Hi Histonet folks,

             Thank you for all the help, for those who are following what I
have found:

(1)



Sigma-Aldrich does have p16 (it is also know as INKa):



Anti-INKa(p16) antibody, clone 13H4.1   cat # MABE1328

or

Anti-p16 Antibody, clone D25  cat#MAB4133



(2)



Stacy was kind enough to share this:





Hi Cassie,
Are you referring to Ventana?
If so, they will have 2 other kits coming out for it.  They will require the
use of Optiview detection or it will be considered an LDT.  Just found this
out from my rep.
Thanks,
Stacy

Stacy McLaughlin, HT (ASCP)
Histology Supervisor
Cooley Dickinson Healthcare
30 Locust Street
Northampton, MA 01060
(413-582-2019



****Stacy, thanks for sharing! We did not get that information from our rep.


Cassandra Davis
Histology Technician
AP Laboratory
302-575-8095
Email:  CDavis at che-east.org<mailto:CDavis at che-east.org>


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