From jqb7 at cdc.gov Mon Jan 2 09:01:56 2017 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Mon, 2 Jan 2017 15:01:56 +0000 Subject: [Histonet] Automated special stains Message-ID: <8951c8a8fb9543f4a7516fe7c587e250@cdc.gov> Happy New Year everyone! I am hoping to get some feedback re: automated special stains instrumentation. We currently have the Artisan but I would like feedback from those using different models...brands. We primarily need one for ZN AF, Gram, W-S (or similar) and GMS. Thanks so much! Jeanine Sanders Centers for Disease Control and Prevention 1600 Clifton Rd. MS-G-32 Atlanta, Ga 30329 404-639-3590 From bcooper at chla.usc.edu Mon Jan 2 13:31:18 2017 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Mon, 2 Jan 2017 19:31:18 +0000 Subject: [Histonet] Automated special stains In-Reply-To: <8951c8a8fb9543f4a7516fe7c587e250@cdc.gov> References: <8951c8a8fb9543f4a7516fe7c587e250@cdc.gov> Message-ID: I tested both the Benchmarks and Artisans two years ago. By far, the Artisans were the clear winner on everything I considered to be comparable criteria (cost, waste handling, ease of use, reliability etc . . .) If you're interested, I can send you the comparison document I drew up. The only stain that was superior on the Benchmark platform was the Retic. Both platforms offer Gram stain; it doesn't work well on either the Benchmark or Artisan (we continue to do Grams by hand). -----Original Message----- From: Sanders, Jeanine (CDC/OID/NCEZID) via Histonet [histonet at lists.utsouthwestern.edu] Received: Monday, 02 Jan 2017, 7:02AM To: 'histonet at lists.utsouthwestern.edu' [histonet at lists.utsouthwestern.edu] Subject: [Histonet] Automated special stains Happy New Year everyone! I am hoping to get some feedback re: automated special stains instrumentation. We currently have the Artisan but I would like feedback from those using different models...brands. We primarily need one for ZN AF, Gram, W-S (or similar) and GMS. Thanks so much! Jeanine Sanders Centers for Disease Control and Prevention 1600 Clifton Rd. MS-G-32 Atlanta, Ga 30329 404-639-3590 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From naje1972 at yahoo.com Mon Jan 2 16:06:52 2017 From: naje1972 at yahoo.com (cynthia haynes) Date: Mon, 2 Jan 2017 22:06:52 +0000 (UTC) Subject: [Histonet] Part time /As needed position References: <547270717.5857724.1483394812048.ref@mail.yahoo.com> Message-ID: <547270717.5857724.1483394812048@mail.yahoo.com> I am in need of someone to provide coverage for ?sick or vacation days. This person will also be offered weekends as well. It would be every other weekend. I am located in Chicago, Illinois. I can be reached at naje1972 at yahoo.com or 312-919-9887.Thank you in advancedCynthia HJames H.T. From naje1972 at yahoo.com Mon Jan 2 16:11:01 2017 From: naje1972 at yahoo.com (cynthia haynes) Date: Mon, 2 Jan 2017 22:11:01 +0000 (UTC) Subject: [Histonet] HMGCoA Reductase analysis References: <924874531.5824479.1483395061531.ref@mail.yahoo.com> Message-ID: <924874531.5824479.1483395061531@mail.yahoo.com> I have a researcher in need of a lab that is doing HMGCoA Reductase procedures, if you are doing this type of staining; please contact me at naje1972 at yahoo.com or 312-919-9887.Thank youCynthia Haynes-James H.T. From tbraud at holyredeemer.com Tue Jan 3 07:43:06 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 3 Jan 2017 13:43:06 +0000 Subject: [Histonet] Dispensing formalin Message-ID: <48E053DDF6CE074DB6A7414BA05403F81192B8@HRHEX03-HOS.holyredeemer.local> For those of you working in smaller hospital Histology labs (not staffed 24/7) - How does your OR/Lab handle getting specimens into fixative? We currently use small/med prefilled containers for smaller specimens. Does the OR dispense formalin into larger containers? Do you refrigerate unfixed specimens? How do you handle specimens collected after pathology hours or on weekends/holidays if they are not fixed? I'm just looking for alternative processes in trying to improve ours. Thanks! Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From rjr6 at psu.edu Tue Jan 3 12:05:15 2017 From: rjr6 at psu.edu (Roberta Horner) Date: Tue, 3 Jan 2017 18:05:15 +0000 Subject: [Histonet] grad student problem Message-ID: <483e26a11daa4ac3a94c34e0259fdb2b@PSU.EDU> I got the following from a grad student here at Penn State. I am not sure how to solve his problem if possible. Does anyone have any suggestions I can forward? Roberta Horner Animal Diagnostic Lab Penn State University "I am having some difficulties sectioning mouse tumor samples for immunofluorescent analysis. We originally went the OCT route because we are staining for T cell markers and were worried that the heating that occurs during paraffin embedding would compromise the T cell receptor. The samples are a little old, but we are hoping to section and stain for immune cell infiltrates. When sectioning with the cryostat, the tissue and OCT is quite brittle and the sample is not intact enough to transfer to a slide. Two colleagues have given the following suggestions: 1. Thaw the OCT blocks, remove the tissue, and place in a new block with fresh OCT media. I've tried this technique on a few practice samples and the new OCT media seems to be less brittle and I'm able to get tissue on a slide, however the tissue itself still seems to be poor with either freeze or sectioning artifacts. 2. Thaw the OCT blocks in water, remove the tissue and place in formalin overnight, place in 70% EtOH, then paraffin embed. Section on a microtome. Check the fluorescently labelled antibody data sheet to see if paraffin embedding interferes with binding. Try to stain and see what happens. I was hesitant to try the second suggestion because I have found no protocol that takes tissue originally stored in OCT blocks and subsequently redirects them to formalin and paraffin for microtome sectioning. If you have any recommendations on how to move forward and section these difficult samples, or know anyone at the diagnostics lab or at Penn State that could help, that would be much appreciated!" From mills at 3scan.com Tue Jan 3 13:01:54 2017 From: mills at 3scan.com (Caroline Miller) Date: Tue, 3 Jan 2017 11:01:54 -0800 Subject: [Histonet] grad student problem In-Reply-To: <483e26a11daa4ac3a94c34e0259fdb2b@PSU.EDU> References: <483e26a11daa4ac3a94c34e0259fdb2b@PSU.EDU> Message-ID: I hate to say it but I think these tissues are toast. It seems they had bad fixation or handling to start with, and I would question whether the tissues were left out to dry before fixation or freezing. Or whether they got fixed and then sucrose infiltrated (which is my method of choice if the antibody allows) Also - what type of tissues? If it is lymph nodes then maybe there is too much fatty tissue and that is why it is not sectioning. I have had a hard time with T-cell markers in paraffin so I do see the hesitation in paraffin embedding. If you do go that route then certainly thaw in formalin, not straight water. Not much help, sorry! But sometimes it is just better to go back and plan the experiment properly in the first place than trying to mess around with old bits of tissue - because even if you could get it on the slide, would you really trust the results of the immunostaining??? Happy New Year All! yours, mills On Tue, Jan 3, 2017 at 10:05 AM, Roberta Horner via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I got the following from a grad student here at Penn State. I am not sure > how to solve his problem if possible. Does anyone have any suggestions I > can forward? > Roberta Horner > Animal Diagnostic Lab > Penn State University > > "I am having some difficulties sectioning mouse tumor samples for > immunofluorescent analysis. We originally went the OCT route because we > are staining for T cell markers and were worried that the heating that > occurs during paraffin embedding would compromise the T cell receptor. The > samples are a little old, but we are hoping to section and stain for immune > cell infiltrates. When sectioning with the cryostat, the tissue and OCT is > quite brittle and the sample is not intact enough to transfer to a slide. > Two colleagues have given the following suggestions: > 1. Thaw the OCT blocks, remove the tissue, and place in a new block with > fresh OCT media. I've tried this technique on a few practice samples and > the new OCT media seems to be less brittle and I'm able to get tissue on a > slide, however the tissue itself still seems to be poor with either freeze > or sectioning artifacts. > 2. Thaw the OCT blocks in water, remove the tissue and place in formalin > overnight, place in 70% EtOH, then paraffin embed. Section on a > microtome. Check the fluorescently labelled antibody data sheet to see if > paraffin embedding interferes with binding. Try to stain and see what > happens. > > I was hesitant to try the second suggestion because I have found no > protocol that takes tissue originally stored in OCT blocks and subsequently > redirects them to formalin and paraffin for microtome sectioning. If you > have any recommendations on how to move forward and section these difficult > samples, or know anyone at the diagnostics lab or at Penn State that could > help, that would be much appreciated!" > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From lldewe at gmail.com Tue Jan 3 16:04:54 2017 From: lldewe at gmail.com (Loralei Dewe) Date: Tue, 3 Jan 2017 14:04:54 -0800 Subject: [Histonet] grad student problem In-Reply-To: References: <483e26a11daa4ac3a94c34e0259fdb2b@PSU.EDU> Message-ID: Completely agree...it sounds like the tissue got freeze dried and I doubt if any staining you might get after lots of work will be completely reliable. Loralei On Jan 3, 2017 11:02 AM, "Caroline Miller via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > I hate to say it but I think these tissues are toast. It seems they had bad > fixation or handling to start with, and I would question whether the > tissues were left out to dry before fixation or freezing. Or whether they > got fixed and then sucrose infiltrated (which is my method of choice if the > antibody allows) > > Also - what type of tissues? If it is lymph nodes then maybe there is too > much fatty tissue and that is why it is not sectioning. > > I have had a hard time with T-cell markers in paraffin so I do see the > hesitation in paraffin embedding. If you do go that route then certainly > thaw in formalin, not straight water. > > Not much help, sorry! But sometimes it is just better to go back and plan > the experiment properly in the first place than trying to mess around with > old bits of tissue - because even if you could get it on the slide, would > you really trust the results of the immunostaining??? > > Happy New Year All! > > yours, > mills > > On Tue, Jan 3, 2017 at 10:05 AM, Roberta Horner via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > I got the following from a grad student here at Penn State. I am not sure > > how to solve his problem if possible. Does anyone have any suggestions I > > can forward? > > Roberta Horner > > Animal Diagnostic Lab > > Penn State University > > > > "I am having some difficulties sectioning mouse tumor samples for > > immunofluorescent analysis. We originally went the OCT route because we > > are staining for T cell markers and were worried that the heating that > > occurs during paraffin embedding would compromise the T cell receptor. > The > > samples are a little old, but we are hoping to section and stain for > immune > > cell infiltrates. When sectioning with the cryostat, the tissue and OCT > is > > quite brittle and the sample is not intact enough to transfer to a slide. > > Two colleagues have given the following suggestions: > > 1. Thaw the OCT blocks, remove the tissue, and place in a new block with > > fresh OCT media. I've tried this technique on a few practice samples and > > the new OCT media seems to be less brittle and I'm able to get tissue on > a > > slide, however the tissue itself still seems to be poor with either > freeze > > or sectioning artifacts. > > 2. Thaw the OCT blocks in water, remove the tissue and place in formalin > > overnight, place in 70% EtOH, then paraffin embed. Section on a > > microtome. Check the fluorescently labelled antibody data sheet to see > if > > paraffin embedding interferes with binding. Try to stain and see what > > happens. > > > > I was hesitant to try the second suggestion because I have found no > > protocol that takes tissue originally stored in OCT blocks and > subsequently > > redirects them to formalin and paraffin for microtome sectioning. If you > > have any recommendations on how to move forward and section these > difficult > > samples, or know anyone at the diagnostics lab or at Penn State that > could > > help, that would be much appreciated!" > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Caroline Miller (mills) > Director of Histology > 3Scan.com > 415 2187297 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From allanvv at gmail.com Tue Jan 3 23:45:17 2017 From: allanvv at gmail.com (Allan Wang) Date: Wed, 4 Jan 2017 00:45:17 -0500 Subject: [Histonet] Dirty H&E after break Message-ID: Hello all, Does someone know what would cause dirty flakes of light purple that showed across the whole slide? http://i.imgur.com/1nIXwAy.jpg Reagents are relatively fresh but the H&E stainer hadn't been used for a week over the holiday break. Is it surface film on the hematoxylin? I didn't notice anything in the container. We are using Mayer's, followed by differentiation with HCl acid alcohol, with 2-3 minute long wash steps in between. Thanks, Allan From tony.henwood at health.nsw.gov.au Wed Jan 4 00:04:15 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 4 Jan 2017 06:04:15 +0000 Subject: [Histonet] Dirty H&E after break In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF014D8FEE@SVDCMBX-MEX008.nswhealth.net> Hi Allan, The flakes are alum precipitates. I would suggest filtering the haematoxylin before use. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Allan Wang via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 4 January 2017 4:45 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Dirty H&E after break Hello all, Does someone know what would cause dirty flakes of light purple that showed across the whole slide? http://i.imgur.com/1nIXwAy.jpg Reagents are relatively fresh but the H&E stainer hadn't been used for a week over the holiday break. Is it surface film on the hematoxylin? I didn't notice anything in the container. We are using Mayer's, followed by differentiation with HCl acid alcohol, with 2-3 minute long wash steps in between. Thanks, Allan _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From edonato at fredhutch.org Wed Jan 4 11:05:17 2017 From: edonato at fredhutch.org (Donato, Elizabeth M) Date: Wed, 4 Jan 2017 17:05:17 +0000 Subject: [Histonet] Beecher (Estigen) ATA27 tissue arrayer for sale Message-ID: Hello Everyone, We are selling our Beecher (now Estigen) ATA 27 automated tissue arrayer and before I post it on re-sale websites, I'd like to share this information with the Histonet community. This instrument was completely re-built/refurbished in 2012 and I have all supporting documentation. It's essentially a brand new instrument. TMA blocks can be constructed using cores of various diameters ranging in size from 0.6mm to 3.0 mm. Cores (0.6mm) can be transferred at a rate of approximately 120-180 cores per hours and up to 26 replicate TMA blocks can be constructed during a single run. This robotic unit can be fully controlled via a high-performance PC pre-loaded with custom software for mapping donor cores to a single or multiple recipient blocks. It has an external video camera which is an upgraded feature. Using this TMA construction software this external camera allows you to superimpose the image of a target marked H&E over the image of the corresponding paraffin block surface for accurate donor core targeting. The user interface is straightforward and gives the you complete control over optimizing variables to accommodate variations in tissue characteristics between donor blocks. Once the TMA grid has been created and the donor blocks mapped using the software, no further user interaction is needed. The nature of our TMA projects has changed over the last few years and this instrument is now underutilized. It needs to be in a lab where it can be used at full capacity. I have a recent quote for a new ATA27 at a cost of 180K, so if you are looking to purchase high throughput TMA construction equipment, this instrument could save you a significant amount of money. We are still in the process of establishing a selling price. If you'd like additional information, pictures or a demonstration of this instrument's capabilities, please contact me. Best, Liz Elizabeth Donato MT(ASCP) Manager | Porter and Specialized Pathology Laboratories ********************************************************************************************* 206.667.4501 p | 206.667.5815 f | edonato at FredHutch.org Fred Hutchinson Cancer Research Center 1100 Fairview Ave. N., Mail Stop C1-015 Seattle, WA 98109 fredhutch.org From CDavis at che-east.org Wed Jan 4 12:24:40 2017 From: CDavis at che-east.org (Cassie P. Davis) Date: Wed, 4 Jan 2017 18:24:40 +0000 Subject: [Histonet] p16 replacements? Message-ID: <5C815EADE724D14AA0CC8F037C4185F079B42821@SB01MSTMBX13.sb.trinity-health.org> Hi Histonet Folks, our current vendor will be discontinuing carrying the antibody for p16. Does anybody have suggestions for replacing in it? We currently use it to stain for CIN. Many thanks in advance for sharing. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From angie at ka-recruiting.com Wed Jan 4 12:40:45 2017 From: angie at ka-recruiting.com (Angie Laparidis) Date: Wed, 4 Jan 2017 13:40:45 -0500 Subject: [Histonet] Greenbay Histology Opportunities with growing lab Message-ID: Happy New Year HistoNet Users! I send out an email last year with news of an expanding client in the *Greenbay, WI* area. They successfully filled their opening, and have some good news for the new year....they are continuing to grow!! This private lab has a new opening on their 2nd shift as well as a new opening on their 3rd shift. Their compensation and benefits packages are unmatched and they truly take care of their employees! Let me know if you can think of anyone who might be a good fit:) Sincerely, *Angie Laparidis*Healthcare Recruiter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South, Boston, MA, 02109 W: 617.746-2744 (*please note this is a new number*) F: (617) 507-8009 Angie at ka-recruiting.com Our openings are updated daily at www.ka-recruiting.com From tbraud at holyredeemer.com Wed Jan 4 13:16:21 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 4 Jan 2017 19:16:21 +0000 Subject: [Histonet] Grad Student Problem Message-ID: <48E053DDF6CE074DB6A7414BA05403F811991C@HRHEX03-HOS.holyredeemer.local> My response: My first question is what temperature are the blocks that are too brittle? And at what temperature are you trying to cut? Blocks stored in cryofreezers at -70o C or less are far too cold to cut without brittleness. My suggestion would be to pull the blocks and put them into the cryostat at -18o C for at least 6 hours to acclimate to that temp, then try to cut and stain for IF. If that doesn't work, then I would thaw in formalin and process as routine tissue for formalin fixed paraffin embedded. The process that your student described in the 2nd step is not a complete, or valid process. Depending on the T-Cell markers they are trying to demonstrate, one can successfully use standard IHC with appropriate clones, or with usually less success, use a modified IF stain procedure for FFPE sections. Since there are a "ba-zillion" T-cell markers, any further details are very dependent on the markers and the condition of the tissue. Sincerely, Terri Braud Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. grad student problem (Roberta Horner) Message: 1 Date: Tue, 3 Jan 2017 18:05:15 +0000 From: Roberta Horner I got the following from a grad student here at Penn State. I am not sure how to solve his problem if possible. Does anyone have any suggestions I can forward? Roberta Horner Animal Diagnostic Lab Penn State University "I am having some difficulties sectioning mouse tumor samples for immunofluorescent analysis. We originally went the OCT route because we are staining for T cell markers and were worried that the heating that occurs during paraffin embedding would compromise the T cell receptor. The samples are a little old, but we are hoping to section and stain for immune cell infiltrates. When sectioning with the cryostat, the tissue and OCT is quite brittle and the sample is not intact enough to transfer to a slide. Two colleagues have given the following suggestions: 1. Thaw the OCT blocks, remove the tissue, and place in a new block with fresh OCT media. I've tried this technique on a few practice samples and the new OCT media seems to be less brittle and I'm able to get tissue on a slide, however the tissue itself still seems to be poor with either freeze or sectioning artifacts. 2. Thaw the OCT blocks in water, remove the tissue and place in formalin overnight, place in 70% EtOH, then paraffin embed. Section on a microtome. Check the fluorescently labelled antibody data sheet to see if paraffin embedding interferes with binding. Try to stain and see what happens. I was hesitant to try the second suggestion because I have found no protocol that takes tissue originally stored in OCT blocks and subsequently redirects them to formalin and paraffin for microtome sectioning. If you have any recommendations on how to move forward and section these difficult samples, or know anyone at the diagnostics lab or at Penn State that could help, that would be much appreciated!" ************* From jpiche at wtbyhosp.org Wed Jan 4 13:23:04 2017 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Wed, 4 Jan 2017 19:23:04 +0000 Subject: [Histonet] Questions regarding microwave questions on Anatomic Pathology CAP Checklist Message-ID: <631955447A364B45B9458D2905635110010779CEDB@WIN08-MBX-01.wtbyhosp.org> Happy New Year Everyone!! :) Questions on CAP Anatomic Path Checklist. Are the microwave questions referring to microwave processors/processing or the microwave oven that we use for special stains? Questions are ANP. 27170, 28290, 28860, and 29430. I'm thinking the microwave oven but I want to confirm or deny. Thanks in advance, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From b-frederick at northwestern.edu Wed Jan 4 14:01:59 2017 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Wed, 4 Jan 2017 20:01:59 +0000 Subject: [Histonet] Fibroblasts Message-ID: <06fb56174fcf4d43b9c8db58b3da7d01@evcspmbx03.ads.northwestern.edu> Hello all, Don't have a ton of time to search so am asking. Any good markers specific to fibroblasts? And am drawing a blank for regular special stains for some reason. Researcher says Vimentin is not specific enough..... Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu From thisisann at aol.com Wed Jan 4 14:14:33 2017 From: thisisann at aol.com (Ann Specian) Date: Wed, 4 Jan 2017 15:14:33 -0500 Subject: [Histonet] Histology school in NJ Message-ID: <1596b1e6ca1-287-325ae@webprd-a16.mail.aol.com> Does anyone know where to find the requirements to start aschool for Histotechnology in nj? Ann From liz at premierlab.com Wed Jan 4 17:10:30 2017 From: liz at premierlab.com (Elizabeth Chlipala) Date: Wed, 4 Jan 2017 16:10:30 -0700 Subject: [Histonet] Fibroblasts In-Reply-To: <06fb56174fcf4d43b9c8db58b3da7d01@evcspmbx03.ads.northwestern.edu> References: <06fb56174fcf4d43b9c8db58b3da7d01@evcspmbx03.ads.northwestern.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE0302AB13D1E1@SBS2K8.premierlab.local> Bernice There are lots of different markers for fibroblasts via IHC - If you are looking for a good source Acris Antibodies has quite a few fibroblast markers Vimentin, Smooth Muscle Actin FSP-1 (fibroblast specific protein) Pro Collagen I CD34 Prolyl-4-Hydroxylase beta HSP-47 I have a two page document that I will send to you that lists a lot of what I have already and what types of fibroblasts that they stain for - we have used most of these in various species. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Bernice Frederick via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, January 04, 2017 1:02 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fibroblasts Hello all, Don't have a ton of time to search so am asking. Any good markers specific to fibroblasts? And am drawing a blank for regular special stains for some reason. Researcher says Vimentin is not specific enough..... Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz at premierlab.com Wed Jan 4 17:14:20 2017 From: liz at premierlab.com (Elizabeth Chlipala) Date: Wed, 4 Jan 2017 16:14:20 -0700 Subject: [Histonet] Grad Student Problem In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F811991C@HRHEX03-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F811991C@HRHEX03-HOS.holyredeemer.local> Message-ID: <14E2C6176416974295479C64A11CB9AE0302AB13D1E3@SBS2K8.premierlab.local> You can also thaw and refreeze we have done that before. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Terri Braud via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, January 04, 2017 12:16 PM To: 'histonet at lists.utsouthwestern.edu' Cc: 'rjr6 at psu.edu' Subject: Re: [Histonet] Grad Student Problem My response: My first question is what temperature are the blocks that are too brittle? And at what temperature are you trying to cut? Blocks stored in cryofreezers at -70o C or less are far too cold to cut without brittleness. My suggestion would be to pull the blocks and put them into the cryostat at -18o C for at least 6 hours to acclimate to that temp, then try to cut and stain for IF. If that doesn't work, then I would thaw in formalin and process as routine tissue for formalin fixed paraffin embedded. The process that your student described in the 2nd step is not a complete, or valid process. Depending on the T-Cell markers they are trying to demonstrate, one can successfully use standard IHC with appropriate clones, or with usually less success, use a modified IF stain procedure for FFPE sections. Since there are a "ba-zillion" T-cell markers, any further details are very dependent on the markers and the condition of the tissue. Sincerely, Terri Braud Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. grad student problem (Roberta Horner) Message: 1 Date: Tue, 3 Jan 2017 18:05:15 +0000 From: Roberta Horner I got the following from a grad student here at Penn State. I am not sure how to solve his problem if possible. Does anyone have any suggestions I can forward? Roberta Horner Animal Diagnostic Lab Penn State University "I am having some difficulties sectioning mouse tumor samples for immunofluorescent analysis. We originally went the OCT route because we are staining for T cell markers and were worried that the heating that occurs during paraffin embedding would compromise the T cell receptor. The samples are a little old, but we are hoping to section and stain for immune cell infiltrates. When sectioning with the cryostat, the tissue and OCT is quite brittle and the sample is not intact enough to transfer to a slide. Two colleagues have given the following suggestions: 1. Thaw the OCT blocks, remove the tissue, and place in a new block with fresh OCT media. I've tried this technique on a few practice samples and the new OCT media seems to be less brittle and I'm able to get tissue on a slide, however the tissue itself still seems to be poor with either freeze or sectioning artifacts. 2. Thaw the OCT blocks in water, remove the tissue and place in formalin overnight, place in 70% EtOH, then paraffin embed. Section on a microtome. Check the fluorescently labelled antibody data sheet to see if paraffin embedding interferes with binding. Try to stain and see what happens. I was hesitant to try the second suggestion because I have found no protocol that takes tissue originally stored in OCT blocks and subsequently redirects them to formalin and paraffin for microtome sectioning. If you have any recommendations on how to move forward and section these difficult samples, or know anyone at the diagnostics lab or at Penn State that could help, that would be much appreciated!" ************* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cls71877 at gmail.com Wed Jan 4 20:27:53 2017 From: cls71877 at gmail.com (Cristi Rigazio) Date: Wed, 4 Jan 2017 21:27:53 -0500 Subject: [Histonet] ANP.22780 and validation Message-ID: <6FEBA632-1574-4409-8D8F-7DA3ABD9819A@gmail.com> Hi Histoworld and Happy New Year! I am currently validating new processing schedules and am a little concerned about my understanding. NSH guidelines don't say special stains, IHCs etc., need to be re validated when validating a new processor, schedules etc., but this CAP standard seems to imply it is required. This, in turn, doesn't make sense to me as many labs send their blocks or unstained slides for subsequent testing to other labs. Those labs don't know the processing schedule, equipment, etc., Can someone shed some light on this for me? Thank you, Cristi Sent from my iPhone From CDavis at che-east.org Thu Jan 5 09:02:25 2017 From: CDavis at che-east.org (Cassie P. Davis) Date: Thu, 5 Jan 2017 15:02:25 +0000 Subject: [Histonet] what I have found about p16 Message-ID: <5C815EADE724D14AA0CC8F037C4185F079B428C6@SB01MSTMBX13.sb.trinity-health.org> Hi Histonet folks, Thank you for all the help, for those who are following what I have found: (1) Sigma-Aldrich does have p16 (it is also know as INKa): Anti-INKa(p16) antibody, clone 13H4.1 cat # MABE1328 or Anti-p16 Antibody, clone D25 cat#MAB4133 (2) Stacy was kind enough to share this: Hi Cassie, Are you referring to Ventana? If so, they will have 2 other kits coming out for it. They will require the use of Optiview detection or it will be considered an LDT. Just found this out from my rep. Thanks, Stacy Stacy McLaughlin, HT (ASCP) Histology Supervisor Cooley Dickinson Healthcare 30 Locust Street Northampton, MA 01060 (413-582-2019 ****Stacy, thanks for sharing! We did not get that information from our rep. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From garethdavisyuma at gmail.com Thu Jan 5 10:10:46 2017 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Thu, 5 Jan 2017 09:10:46 -0700 Subject: [Histonet] pathologist adding IHC stains Message-ID: How are labs indicating, on reports, that the pathologist has ordered IHC stains after reading the H&E slides. With the new CAP rules regarding ordering IHC stains, i.e. H. pylori and others, that are usually ordered initially. I basically would like an example of wording for the reports, when the pathologist does order a stain. Any suggestions? Thanks -- Ms. Gareth B. Davis, HT, QIHC (ASCPcm) Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From greg.dobbin at gmail.com Thu Jan 5 10:12:48 2017 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Thu, 5 Jan 2017 12:12:48 -0400 Subject: [Histonet] grad student problem Message-ID: I can't speak to the the T-cell receptor sites but the only option for getting decent sections at this point is option number two. In the future, (if there applicable) the cut surface of a cryosectioned block can be recovered with OCT, frozen, and stored in an air-tight whirl-pak or ziplock bag in the freezer for a little longer to avoid freezer burn. Cheers, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From garethdavisyuma at gmail.com Thu Jan 5 11:47:12 2017 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Thu, 5 Jan 2017 10:47:12 -0700 Subject: [Histonet] correction on CAP policy Message-ID: After researching and calling CAP I have realized it is not a CAP policy for pathologist to order IHC/SS only after viewing the H&E. But, it is an active LCD. I am new to regulations in labs, being my first time to run one. Pardon my mistake and thanks for the responses. -- Ms. Gareth B. Davis, HT, QIHC (ASCPcm) Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From lmarie08 at uga.edu Fri Jan 6 10:52:32 2017 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Fri, 6 Jan 2017 16:52:32 +0000 Subject: [Histonet] IHC question Message-ID: Hello Histonet world, Our lab has an old Dako Autostainer for IHC and there is a leak in the y-axis arm at the point where the tubing goes through a juncture. I was wondering if there is anyone out there that uses this in there lab? If so, where do you get spare parts for it? Our PI wants us to try to replace the tubing ourselves to see if that fixes the problem before getting service. Thanks! Lauren Lauren Sweeney PDRC Pathology Laboratory Manager University of Georgia | College of Veterinary Medicine Department of Population Health 953 College Station Rd | Athens, GA 30605 706-583-0636 | lmarie08 at uga.edu From CDavis at che-east.org Fri Jan 6 11:25:40 2017 From: CDavis at che-east.org (Cassie P. Davis) Date: Fri, 6 Jan 2017 17:25:40 +0000 Subject: [Histonet] Questions RE: "what I have found about p16" and cellblocks for controls Message-ID: <5C815EADE724D14AA0CC8F037C4185F079B429CB@SB01MSTMBX13.sb.trinity-health.org> Is there anyone in Histonet land who is using both the Optiview and Ultraview on the XT and ULTRA at the same time? I'd like to ask a few questions, please email me. We have a cytology cellblock case that came out stunningly positive for Her2 and one of our pathologist thought it would make a dynamic control if there were any specimen left to make the control block. While I am excited about the possiblity (Her2 control is hard to come by around here) some concern is expressed about using it because it is initally fixed in cytolyt before FFPE. I can argue both sides of this. I would enjoy your input on this folks. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org ________________________________ From: Cassie P. Davis Sent: Thursday, January 05, 2017 10:02 AM To: histonet at lists.utsouthwestern.edu Subject: what I have found about p16 Hi Histonet folks, Thank you for all the help, for those who are following what I have found: (1) Sigma-Aldrich does have p16 (it is also know as INKa): Anti-INKa(p16) antibody, clone 13H4.1 cat # MABE1328 or Anti-p16 Antibody, clone D25 cat#MAB4133 (2) Stacy was kind enough to share this: Hi Cassie, Are you referring to Ventana? If so, they will have 2 other kits coming out for it. They will require the use of Optiview detection or it will be considered an LDT. Just found this out from my rep. Thanks, Stacy Stacy McLaughlin, HT (ASCP) Histology Supervisor Cooley Dickinson Healthcare 30 Locust Street Northampton, MA 01060 (413-582-2019 ****Stacy, thanks for sharing! We did not get that information from our rep. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From elaineahoffman55 at yahoo.com Fri Jan 6 14:20:32 2017 From: elaineahoffman55 at yahoo.com (Elaine allison Hoffman) Date: Fri, 6 Jan 2017 20:20:32 +0000 (UTC) Subject: [Histonet] Quality Assessment (QA) References: <1452858503.1446780.1483734032911.ref@mail.yahoo.com> Message-ID: <1452858503.1446780.1483734032911@mail.yahoo.com> Hello Histonetter's and Happy New Year! I have a question concerning Histology Quality Assessment (QA) and what that would look like.? We are a tiny GI histology lab that has been opened for only a few years.? My last CLIA inspection last month went well, but the inspector said that we needed to have a QA "proactive reviewof all phases of the testing process....preanalytic, analytic, postanalytic, and general laboratory systems".? In other words, she said "it's like doing a mini CLIA inspection on yourselves".? My question is how is the histology lab doing quality assessment (QA) and what does that look like?? I need some suggestions on how other histology labs are doing that and how is it documented???I'm still learning these requirements on my own and don't really have anyone to guide me.? Any feedback would be greatly appreciated.? Thank you in advance for your help.? Elaine From allanvv at gmail.com Fri Jan 6 23:01:08 2017 From: allanvv at gmail.com (Allan Wang) Date: Sat, 7 Jan 2017 00:01:08 -0500 Subject: [Histonet] Dirty H&E after break In-Reply-To: <0237449DE79DBC45B686AB82CDCD16FF014D8FEE@SVDCMBX-MEX008.nswhealth.net> References: <0237449DE79DBC45B686AB82CDCD16FF014D8FEE@SVDCMBX-MEX008.nswhealth.net> Message-ID: Yep, filtering the hematoxlyn fixed it. Thanks for the tip. Is it worthwhile to filter the eosin, alcohols, and xylene substitutes as well? Allan On Jan 4, 2017 1:04 AM, "Tony Henwood (SCHN)" < tony.henwood at health.nsw.gov.au> wrote: > Hi Allan, > > The flakes are alum precipitates. > > I would suggest filtering the haematoxylin before use. > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: Allan Wang via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, 4 January 2017 4:45 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Dirty H&E after break > > Hello all, > > Does someone know what would cause dirty flakes of light purple that > showed across the whole slide? > > http://i.imgur.com/1nIXwAy.jpg > > Reagents are relatively fresh but the H&E stainer hadn't been used for a > week over the holiday break. > Is it surface film on the hematoxylin? I didn't notice anything in the > container. We are using Mayer's, followed by differentiation with HCl acid > alcohol, with 2-3 minute long wash steps in between. > > Thanks, > Allan > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain > confidential information. If you are not the intended recipient, please > delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and > are not necessarily the views of NSW Health or any of its entities. > > From gu.lang at gmx.at Sat Jan 7 01:48:56 2017 From: gu.lang at gmx.at (Gudrun Lang) Date: Sat, 7 Jan 2017 08:48:56 +0100 Subject: [Histonet] Questions RE: "what I have found about p16" and cellblocks for controls In-Reply-To: <5C815EADE724D14AA0CC8F037C4185F079B429CB@SB01MSTMBX13.sb.trinity-health.org> References: <5C815EADE724D14AA0CC8F037C4185F079B429CB@SB01MSTMBX13.sb.trinity-health.org> Message-ID: <000601d268ba$816fcfc0$844f6f40$@gmx.at> Your concerns are reasonable. Cyto-specimens are usually fixed in alcoholic solutions not in NBF. Alcohol-fixation gives false positives in Her2. This can also be seen in underfixed tissue, that is mainly fixed by the ethanols in the processor. Her2-protein is a normal protein on the cellsurface. FFPE treatment "turns it down" and normal amount cannot be detected. This is "negative". Only Her2 positives have abundant protein to be detected with our methods. That is why correct standardizised treatment is important to avoid "false positives" and "false negatives". Gudrun leading histotechnologist, Kepler Universit?tsklinikum Linz Austria -----Urspr?ngliche Nachricht----- Von: Cassie P. Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Freitag, 6. Januar 2017 18:26 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Questions RE: "what I have found about p16" and cellblocks for controls Is there anyone in Histonet land who is using both the Optiview and Ultraview on the XT and ULTRA at the same time? I'd like to ask a few questions, please email me. We have a cytology cellblock case that came out stunningly positive for Her2 and one of our pathologist thought it would make a dynamic control if there were any specimen left to make the control block. While I am excited about the possiblity (Her2 control is hard to come by around here) some concern is expressed about using it because it is initally fixed in cytolyt before FFPE. I can argue both sides of this. I would enjoy your input on this folks. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org ________________________________ From: Cassie P. Davis Sent: Thursday, January 05, 2017 10:02 AM To: histonet at lists.utsouthwestern.edu Subject: what I have found about p16 Hi Histonet folks, Thank you for all the help, for those who are following what I have found: (1) Sigma-Aldrich does have p16 (it is also know as INKa): Anti-INKa(p16) antibody, clone 13H4.1 cat # MABE1328 or Anti-p16 Antibody, clone D25 cat#MAB4133 (2) Stacy was kind enough to share this: Hi Cassie, Are you referring to Ventana? If so, they will have 2 other kits coming out for it. They will require the use of Optiview detection or it will be considered an LDT. Just found this out from my rep. Thanks, Stacy Stacy McLaughlin, HT (ASCP) Histology Supervisor Cooley Dickinson Healthcare 30 Locust Street Northampton, MA 01060 (413-582-2019 ****Stacy, thanks for sharing! We did not get that information from our rep. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Sun Jan 8 16:19:26 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun, 8 Jan 2017 22:19:26 +0000 Subject: [Histonet] Dirty H&E after break In-Reply-To: References: <0237449DE79DBC45B686AB82CDCD16FF014D8FEE@SVDCMBX-MEX008.nswhealth.net> Message-ID: <0237449DE79DBC45B686AB82CDCD16FF014D9860@SVDCMBX-MEX008.nswhealth.net> Hi Allan, With the quality of the eosin dyes today, filtering should not be required. The same for xylene and ethanols. You will need to monitor the usage and solution levels (evaporation being the issue here). There are no hard and fast rules but a suggestion is one slide per ml of solution. Therefore a bath of 400ml of haematoxylin should stain 400 slides consistently before a noticeable deterioration in staining is noted. The same with the other solutions. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Allan Wang [mailto:allanvv at gmail.com] Sent: Saturday, 7 January 2017 4:01 PM To: Tony Henwood (SCHN) Cc: histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] Dirty H&E after break Yep, filtering the hematoxlyn fixed it. Thanks for the tip. Is it worthwhile to filter the eosin, alcohols, and xylene substitutes as well? Allan On Jan 4, 2017 1:04 AM, "Tony Henwood (SCHN)" > wrote: Hi Allan, The flakes are alum precipitates. I would suggest filtering the haematoxylin before use. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Allan Wang via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 4 January 2017 4:45 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Dirty H&E after break Hello all, Does someone know what would cause dirty flakes of light purple that showed across the whole slide? http://i.imgur.com/1nIXwAy.jpg Reagents are relatively fresh but the H&E stainer hadn't been used for a week over the holiday break. Is it surface film on the hematoxylin? I didn't notice anything in the container. We are using Mayer's, followed by differentiation with HCl acid alcohol, with 2-3 minute long wash steps in between. Thanks, Allan _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From tawnia at ampianstaffing.com Tue Jan 10 17:07:40 2017 From: tawnia at ampianstaffing.com (Tawnia Lindsay) Date: Tue, 10 Jan 2017 23:07:40 +0000 Subject: [Histonet] Job opportunites In-Reply-To: References: Message-ID: Hi Hisotnetters! New Year, New Job!!! I am currently looking for a couple of travelers to fill a couple of 13 week assignments. If you are in the market to make a change, please give me a call at 877-229-6996 ext 2009, or email me at tawnia at ampianstaffing.com. Looking forward to helping you make 2017 amazing! -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Monday, January 09, 2017 11:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 158, Issue 7 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Dirty H&E after break (Tony Henwood (SCHN)) ---------------------------------------------------------------------- Message: 1 Date: Sun, 8 Jan 2017 22:19:26 +0000 From: "Tony Henwood (SCHN)" To: Allan Wang Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Dirty H&E after break Message-ID: <0237449DE79DBC45B686AB82CDCD16FF014D9860 at SVDCMBX-MEX008.nswhealth.net> Content-Type: text/plain; charset="utf-8" Hi Allan, With the quality of the eosin dyes today, filtering should not be required. The same for xylene and ethanols. You will need to monitor the usage and solution levels (evaporation being the issue here). There are no hard and fast rules but a suggestion is one slide per ml of solution. Therefore a bath of 400ml of haematoxylin should stain 400 slides consistently before a noticeable deterioration in staining is noted. The same with the other solutions. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Allan Wang [mailto:allanvv at gmail.com] Sent: Saturday, 7 January 2017 4:01 PM To: Tony Henwood (SCHN) Cc: histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] Dirty H&E after break Yep, filtering the hematoxlyn fixed it. Thanks for the tip. Is it worthwhile to filter the eosin, alcohols, and xylene substitutes as well? Allan On Jan 4, 2017 1:04 AM, "Tony Henwood (SCHN)" > wrote: Hi Allan, The flakes are alum precipitates. I would suggest filtering the haematoxylin before use. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Allan Wang via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 4 January 2017 4:45 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Dirty H&E after break Hello all, Does someone know what would cause dirty flakes of light purple that showed across the whole slide? http://i.imgur.com/1nIXwAy.jpg Reagents are relatively fresh but the H&E stainer hadn't been used for a week over the holiday break. Is it surface film on the hematoxylin? I didn't notice anything in the container. We are using Mayer's, followed by differentiation with HCl acid alcohol, with 2-3 minute long wash steps in between. Thanks, Allan _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 158, Issue 7 **************************************** From relia1 at earthlink.net Wed Jan 11 08:03:50 2017 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 11 Jan 2017 09:03:50 -0500 Subject: [Histonet] RELIA HOT JOB ALERT!! Full Time Mohs Histotech needed in Florida ASAP!! Message-ID: <007e01d26c13$8a1f4810$9e5dd830$@earthlink.net> HI Histonetters!! Happy New Year and Happy Hump Day. I have a lot of exciting new opportunities to share but first I want to post specifically about the one I am most excited about!! I am working exclusively with a client here in Florida that is in need of a Mohs tech. this is a well-established and growing dermatology practice. ASCP certification and grossing experience are preferred but not required. They are offering a competitive pay rate, excellent benefits and relocation is negotiable. Some of the people there are friends of mine and I can tell you, this is a great group of people to work with. I am not disclosing the exact location in this post (I don't want to be poached by other recruiters). However if you are interested in more information and or the location just shoot me an email. And Remember. If you know someone who might be interested and refer them to me, I will pay you a referral reward bonus if I place them. Have a great day!!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From ASelf at tidelandshealth.org Thu Jan 12 09:46:49 2017 From: ASelf at tidelandshealth.org (Amy Self) Date: Thu, 12 Jan 2017 10:46:49 -0500 Subject: [Histonet] Slide and Block Storage Message-ID: Happy Thursday connected to Friday, Have a few questions about slide and block storage... Are you blocks and slides stored on-site or off-site at a record control type facility? If your blocks and slides are stored off-site how do you get the material that you need at any given point of the day? Thanks in advance for your help, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From wbenton at cua.md Thu Jan 12 10:01:11 2017 From: wbenton at cua.md (Walter Benton) Date: Thu, 12 Jan 2017 16:01:11 +0000 Subject: [Histonet] Slide and Block Storage In-Reply-To: References: Message-ID: <5e3b30bd63384389b9ff2e1af7e25152@MAIL01.GCU-MD.local> Depending on the contract that you select with your vendor, you can determine a cutoff time for same day deliveries. We order by noon and have the delivery by 3pm the same day. Orders placed after 12pm are delivered on the next business morning. I'm sure there are more rapid options, but they are probably more costly as well. -----Original Message----- From: Amy Self via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, January 12, 2017 10:47 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Storage Happy Thursday connected to Friday, Have a few questions about slide and block storage... Are you blocks and slides stored on-site or off-site at a record control type facility? If your blocks and slides are stored off-site how do you get the material that you need at any given point of the day? Thanks in advance for your help, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From rjbuesa at yahoo.com Thu Jan 12 10:27:40 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 12 Jan 2017 16:27:40 +0000 (UTC) Subject: [Histonet] Slide and Block Storage In-Reply-To: References: Message-ID: <1593446955.2020267.1484238460706@mail.yahoo.com> ?We used to keep our blocks on-site during 9 years. During January of each year we disposed off 1 year worth of blocks (the oldest)?and only kept those?deemed?by the pathologists as interesting?for our residents' training program.Regarding slides, in 2001 we had 56 years of slides on-site and it was decided to send the majority out to a "specialized" facility, and it was a complete nightmare. Many slides broke and the final arrangement was incorrect becoming extraordinarily difficult finding a case.This situation was most likely caused by a poor selection of the storing company. I would never repeat this decision but, on the other hand, you could ask: what is the point in storing slides for so many years?My suggestion to you is to ask your legal department what could be the expectation regarding slides storage, i.e. how many years the lab could be liable if a legal suit is levied against the hospital? This is a difficult question to answer.Other approach could be to follow CAP requirements regarding storage time for blocks and slides. In Florida we are required to store blocks for 9 years, and that is what we did.My recommendation to you is: keep blocks and slides for the maximum time required by your licensing agency, and store them within your actual reach do NOT send them out to store outside. Discard the rest keeping only those that represent a teaching interest.Ren??? On Thursday, January 12, 2017 10:59 AM, Amy Self via Histonet wrote: Happy Thursday connected to Friday, Have a few questions about slide and block storage... Are you blocks and slides stored on-site or off-site at a record control type facility? If your blocks and slides are stored off-site how do you get the material that you need at any given point of the day? Thanks in advance for your help, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen at parrishmed.com Thu Jan 12 11:03:30 2017 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Thu, 12 Jan 2017 12:03:30 -0500 Subject: [Histonet] Slide and Block Storage In-Reply-To: References: Message-ID: <450B7A81EDA0C54E97C53D60F00776C323E455E696@isexstore03> We store both blocks and slides on-site for 10 years which is regulated by CAP. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Amy Self via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, January 12, 2017 10:47 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Slide and Block Storage Happy Thursday connected to Friday, Have a few questions about slide and block storage... Are you blocks and slides stored on-site or off-site at a record control type facility? If your blocks and slides are stored off-site how do you get the material that you need at any given point of the day? Thanks in advance for your help, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From jpiche at wtbyhosp.org Fri Jan 13 08:17:11 2017 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Fri, 13 Jan 2017 14:17:11 +0000 Subject: [Histonet] Harris Hematoxylin Message-ID: <631955447A364B45B9458D2905635110010779D311@WIN08-MBX-01.wtbyhosp.org> Good Morning Everyone, Just wondering what kind of Harris Hematoxylin people are using. We are doing a regressive H&E on a Leica Autostainer XL for 9 minutes. We have been buying Ricca Hematoxylin but we are having so many issues with it. Are people using acidified Hematoxylin in regressive staining protocols? Thank you in advance! :) Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From relia1 at earthlink.net Fri Jan 13 09:17:20 2017 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 13 Jan 2017 10:17:20 -0500 Subject: [Histonet] RELIA Histology Careers Bulletin 01-10-2016 Happy New Year and A Call for Info on State and Regional Society Meetings Message-ID: <008501d26db0$231a8d20$694fa760$@earthlink.net> Hi Histonetters!!! , Happy New Year!!!! Right Place, Right Time, Right Opportunity! Sometimes your next career move is just a matter of being in the right place at the right time and ready for the right opportunity. Odds are that if you are contemplating a job change for whatever reason ? better compensation, a more desirable location or more challenging work, you don?t have the time to do a job search. I would like to offer my help I have clients located throughout the country. All of the jobs that I represent are full time permanent positions with excellent compensation, benefits and relocation/sign-on bonuses. I will assist you with your resume, coordination of interviews and coaching throughout the process. My services are FREE of charge to you. Here is the latest update of the positions I am most excited to represent. If you are interested in any of these positions ? give me a call toll free at 866-607-3542 or shoot me an e-mail at relia1 at earthlink.net and let?s discuss it. Whether you are looking for a new position today, tomorrow or 6 months from now, it is never too early to have me keep a watch out for that perfect job for you! *These Are Our Current Opportunities* Histology Supervisors, Managers and Lead Histotechs: -Santa Ana, CA -Seattle, WA -Cheyenne, WY Histotechnicians and Histotechnologists: -Mohs Histotech - Fort Myers, FL -St Louis, MO ? Mohs Tech ? BRAND NEW LAB!!! -Cheyenne, WY ? BRAND NEW LAB!!! -Seattle, WA ? Lead Tech Train To Become A Supervisor!! -Green Bay, WI -New Grads Welcome To Apply!! -Syracuse, NY ? NY License Required New Grads Welcome!! Exciting Histology Opportunities Also Available In: -Chattanooga, TN -Kingsport, TN -Glenwood, Springs, CO This is a call for information regarding state and regional meetings to be published in the 02/21/2016 RELIA Careers Bulletin (free posting) If you would like the link to your website and or meeting info to be mentioned in the 02/21/2016 RELIA Careers Bulletin please email it to RELIA1 at earthlink.net by 02/17/2016 Remember timing is everything Have a great weekend!! Thanks ? Pam 866-60-RELIA (866-607-3542) Thank You! ? ? Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From relia1 at earthlink.net Fri Jan 13 09:41:54 2017 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 13 Jan 2017 10:41:54 -0500 Subject: [Histonet] RELIA Histology Careers Bulletin 01-13-2017 Happy New Year and A Call for Info on State and Regional Society Meetings OOPS!! Make that 2017!! Message-ID: <008c01d26db3$91544bc0$b3fce340$@earthlink.net> Hi Histonetters!!!, Happy New Year!!!! Right Place, Right Time, Right Opportunity! Sometimes your next career move is just a matter of being in the right place at the right time and ready for the right opportunity. Odds are that if you are contemplating a job change for whatever reason - better compensation, a more desirable location or more challenging work, you don't have the time to do a job search. I would like to offer my help. I have clients located throughout the country. All of the jobs that I represent are full time permanent positions with excellent compensation, benefits and relocation/sign-on bonuses. I will assist you with your resume, coordination of interviews and coaching throughout the process. My services are FREE of charge to you. Here is the latest update of the positions I am most excited to represent. If you are interested in any of these positions - give me a call toll free at 866-607-3542 or shoot me an e-mail at relia1 at earthlink.net and let's discuss it. Whether you are looking for a new position today, tomorrow or 6 months from now, it is never too early to have me keep a watch out for that perfect job for you! *These Are Our Current Opportunities* Histology Supervisors, Managers and Lead Histotechs: Santa Ana, CA Seattle, WA Cheyenne, WY Histotechnicians and Histotechnologists: . St Louis, MO - Mohs Tech - BRAND NEW LAB!!! . Cheyenne, WY - BRAND NEW LAB!!! . San Diego, CA - BRAND NEW LAB!!! . Seattle, WA - Lead Tech Train To Become A Supervisor!! . Green Bay, WI -New Grads Welcome To Apply!! . Syracuse, NY - NY License Required New Grads Welcome!! Exciting Histology Opportunities Also Available In: . Chattanooga, TN . Modesto, CA - IHC Specialist . Kingsport, TN . Glenwood, Springs, CO . Ft. Myers, FL - Part time - Mohs Tech This is a call for information regarding state and regional meetings to be published in the 02/21/2016 RELIA Careers Bulletin (free posting) If you would like the link to your website and or meeting info to be mentioned in the 02/21/2016 RELIA Careers Bulletin please email it to RELIA1 at earthlink.net by 02/17/2016 Remember timing is everything. Thanks - Pam 866-60-RELIA (866-607-3542) Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From ADuddey at firsthealth.org Fri Jan 13 10:25:28 2017 From: ADuddey at firsthealth.org (Duddey, Aimee) Date: Fri, 13 Jan 2017 16:25:28 +0000 Subject: [Histonet] Full time histology technical specialist position available Message-ID: <09FBA01CA9B6374A83C5C76E09E46188A3ED956F@EXMAIL1-FHC.firsthealth.org> Full time position available in Pinehurst, NC Job Title: Histology Technical Specialist Supervised by: Asst. Director of Pathology Job Summary: The Histology Technical Specialist coordinates and monitors the day-to-day workflow of the histology area of pathology, serves as the first line resource for daily operations and assists the Assistant Director of Laboratory for Pathology with schedule management activities and provides technical - level services in the area of expertise. Histotechnologist duties include practicing clinical laboratory science in the specialty of histology to include immunohistochemistry. May be required to perform other duties within the pathology department as directed by the pathologist or assistant director of Pathology. May include clerical duties or duties within the cytology area of the laboratory depending on the availability of personnel. The Histology Technical Specialist works with the Histology Technical supervisor and AD to establish Histology department processes, and reviews, updates and writes histology laboratory procedures to ensure the high quality of specimen preparations. Responsible for recognizing issues or problems that require referral to the Pathologist. Maintains performance improvement activities within the department and participates in quality improvement activities. Coordinates and monitors daily activities of histology workflow; assists AD in activities that may include interviewing, work allocation, orientation, training and competency assessment, contributing to performance evaluations and motivating employees to achieve peak performance. Demonstrates pro-active problem solving skills to include identifying a problem and taking appropriate action; knows when to seek guidance to complete troubleshooting protocol; communicates necessary information to AD. Acts as a resource to customers, both within the department and outside the department. Accepts responsibility for own development to include cross-training in other functions or positions to ensure satisfactory operation of the department or to permit flexible staffing assignments. Regulatory Requirements: 1. High School graduate or equivalent. 2. Graduated from accredited school of histotechnology or medical laboratory technology. 3. Current MLT, MT, HT, or HTL certification, i.e., American Society of Clinical Pathologists. Skills: 1. Demonstrates the ability to coordinate multiple tasks with precision and flexibility to provide accurate results within the minimum turnaround time established by this laboratory. 2. Knowledge and skills related to laboratory computer information systems and word processing is desirable. 3. Must be able to work within a framework of a team concept to accomplish identified mission and goals. 4. Excellent verbal, written, and clerical skills Physical Demands: 1. Ability to carry objects weighing up to 25 pounds. 2. Responsible for lifting, carrying, pushing, and pulling objects involving laboratory related equipment and supplies. 3. Able to withstand sufficient noise and interruptions to cause distractions and stress. 4. Daily functions may include incidental exposure to vapors, fumes, moving mechanical parts, chemical and biological hazards, patient contact, needles/syringes, waste handling, and computer use. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions of the position without compromising client care. Please visit www.firsthealth.org/join-our-team/careers and use keyword histology to find the posting and apply. Thank you for reading, Aimee M. Duddey, MLT(ASCP) Assistant Director of Laboratory - Pathology FH Moore Regional Hospital 910-715-5286 office 910715-1944 fax From rsrichmond at gmail.com Sat Jan 14 20:41:39 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 14 Jan 2017 21:41:39 -0500 Subject: [Histonet] Harris Hematoxylin Message-ID: Jessica Piche, HT(ASCP) at Waterbury Hospital [Connecticut] asks: >>Just wondering what kind of Harris Hematoxylin people are using. We are doing a regressive H&E on a Leica Autostainer XL for 9 minutes. We have been buying Ricca Hematoxylin but we are having so many issues with it. Are people using acidified Hematoxylin in regressive staining protocols?<< How can you be using Harris hematoxylin today? Harris hematoxylin by definition is oxidized with mercuric oxide, and I don't think they make it any more because of the problem of disposing of mercury. I guess the new definition is "any alum hematoxylin our marketing department wants to call Harris". Bob Richmond Samurai Pathologist Maryville TN From ssegal2 at slu.edu Sun Jan 15 12:05:24 2017 From: ssegal2 at slu.edu (ssegal2) Date: Sun, 15 Jan 2017 12:05:24 -0600 Subject: [Histonet] Reichert-Jung Tetrander sliding microtome Message-ID: Greetings Does anybody have access to a user's manual for this device? Thanks Sent from my Verizon, Samsung Galaxy smartphone From logangovender at icloud.com Mon Jan 16 05:05:41 2017 From: logangovender at icloud.com (Logan Govender) Date: Mon, 16 Jan 2017 11:05:41 +0000 (GMT) Subject: [Histonet] Histology/Cytology slide labelling Message-ID: <60b422ed-cb0c-44ba-bf47-78f87b07c129@me.com> If anyone is interested, I have designed and created software for histology laboratories in the following catergories: 1. Histology/Cytology Slide Labelling? (use chemical resistant labels, printed with lab number, sub, stain, path, date) 2. Pathologist online stain request (complete with stain audit trail) Please feel free to contact me for full details. logangovender at icloud.com From patpxs at gmail.com Mon Jan 16 09:46:16 2017 From: patpxs at gmail.com (P Sicurello) Date: Mon, 16 Jan 2017 07:46:16 -0800 Subject: [Histonet] CEs for EM techs? Message-ID: Good Morning, Does anyone know if there are CEs available for electron microscopists? I tried sending this question to the MSA listserver but my email was rejected. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From tmcampbe at fmh.org Mon Jan 16 07:17:59 2017 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Mon, 16 Jan 2017 13:17:59 +0000 Subject: [Histonet] PRN Frederick, MD Message-ID: Hello all, I am looking for a histotechnologist in the Frederick, MD area that would be interested in covering vacations and sick time for a small GI lab. Flexible hours. Easy job. Please email or call if interested. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 From relia1 at earthlink.net Mon Jan 16 11:20:47 2017 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 16 Jan 2017 12:20:47 -0500 Subject: [Histonet] RELIA HOT JOB ALERT!! Run your own BRAND NEW LAB in Cheyenne, WY. Message-ID: <002601d2701c$e1b3d520$a51b7f60$@earthlink.net> Hi Histonetters! Happy Monday. I hope this is the beginning of a fantastic week for you. I wanted to post this position I am working on because it is rare that I see an opportunity like this cross my desk. Here is the info: Lead Histology Tech - Cheyenne, WY I have been engaged by a private lab located in Cheyenne, WY that is in need of an Lead Histology Tech to run their own BRAND NEW LAB!!. ASCP HT/HTL, CLIA qualified to gross and 5-7 years of histology experience is required. Knowledge of lab administrative duties is preferred. My client offers a generous salary, great benefits, relocation assistance and an excellent opportunity. If this is the right job for you RELIA can make it happen! For more information please contact Pam Barker at relia1 at earthlink.net or toll free at 866-607-3542 If you know someone who might be interested please pass along the info. If I hire someone you refer to me you will earn a referral bonus. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From srishan at mail.holyname.org Mon Jan 16 13:10:38 2017 From: srishan at mail.holyname.org (srishan at mail.holyname.org) Date: Mon, 16 Jan 2017 14:10:38 -0500 Subject: [Histonet] blade holder for BP # 60 blades Message-ID: Hi All, Can someone help me find the blade handle that fits BP number 60 blades? It would be greatly appreciated. Thanks Nirmala Srishan Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From alicia.ortega at colorado.edu Mon Jan 16 18:44:56 2017 From: alicia.ortega at colorado.edu (Alicia Marie Ortega) Date: Tue, 17 Jan 2017 00:44:56 +0000 Subject: [Histonet] Buffer concentration in 4% paraformaldehyde Message-ID: Hello everyone, I was wondering if anyone knows if there is a difference between preserving tissue (bone to be specific) in 4% paraformaldehyde made with 10x phosphate buffer versus 1x phosphate buffer? We typically use 1x phosphate buffer when making 4% paraformaldehyde, but have some samples that were preserved in a store bought 4% paraformaldehyde in 10x phosphate buffer, and are wondering what differences this might cause? Thank you in advance for your help. Sincerely, Alicia Ortega Research Associate University of Colorado From mlm11 at cornell.edu Wed Jan 18 08:01:03 2017 From: mlm11 at cornell.edu (Mary Lou Norman) Date: Wed, 18 Jan 2017 14:01:03 +0000 Subject: [Histonet] staining samples years in formalin Message-ID: Dear Histonetters, My samples have been in formalin up to 3 years and there have been complaints about poor staining. Please help me with solutions if there are any. My controls have not been in formalin as long. The complaint has been with the Alcian Blue pH2.5 and Saf O specifically. The samples are stifles so I need to decal them also. I use formic/na citrate. Any and all comments, suggestions are much appreciated. Thank you. Mary Lou Norman From vanessa.keeton03 at gmail.com Wed Jan 18 09:30:35 2017 From: vanessa.keeton03 at gmail.com (Vanessa Keeton) Date: Wed, 18 Jan 2017 10:30:35 -0500 Subject: [Histonet] Releasing of Patient Tissue Message-ID: Good Morning All! I was wondering what everyone's policies were on releasing of patient tissue other than stones, placentas, and hardware. Normally we only release stones, placentas, and hardware but have been receiving request for other types of tissues lately and was just curious as to what the norm seems to be pertaining to this issue and if you do release the tissue, how do you limit patient exposure to blood, body fluids and chemicals? Thanks!!! Vanessa Keeton From rjbuesa at yahoo.com Wed Jan 18 10:25:40 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 18 Jan 2017 16:25:40 +0000 (UTC) Subject: [Histonet] Releasing of Patient Tissue In-Reply-To: References: Message-ID: <705949780.5831751.1484756740422@mail.yahoo.com> Never mind what other people do, just ask your legal department what to do because this may involve legal consequences.Ren?? On Wednesday, January 18, 2017 10:47 AM, Vanessa Keeton via Histonet wrote: Good Morning All! I was wondering what everyone's policies were on releasing of patient tissue other than stones, placentas, and hardware.? Normally we only release stones, placentas, and hardware but have been receiving request for other types of tissues lately and was just curious as to what the norm seems to be pertaining to this issue and if you do release the tissue, how do you limit patient exposure to blood, body fluids and chemicals? Thanks!!! Vanessa Keeton _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Wed Jan 18 12:34:53 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 18 Jan 2017 18:34:53 +0000 Subject: [Histonet] Release of patient tissue Message-ID: <48E053DDF6CE074DB6A7414BA05403F811CDF6@HRHEX03-HOS.holyredeemer.local> Our policy is to not release any wet tissue except by subpoena, except for purposes of burial, and then only released to a funeral director. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 2. Releasing of Patient Tissue (Vanessa Keeton) Date: Wed, 18 Jan 2017 10:30:35 -0500 From: Vanessa Keeton Subject: [Histonet] Releasing of Patient Tissue Good Morning All! I was wondering what everyone's policies were on releasing of patient tissue other than stones, placentas, and hardware. Normally we only release stones, placentas, and hardware but have been receiving request for other types of tissues lately and was just curious as to what the norm seems to be pertaining to this issue and if you do release the tissue, how do you limit patient exposure to blood, body fluids and chemicals? Thanks!!! Vanessa Keeton From tejohnson at genoptix.com Wed Jan 18 12:47:10 2017 From: tejohnson at genoptix.com (Teri Johnson) Date: Wed, 18 Jan 2017 18:47:10 +0000 Subject: [Histonet] Primera slide printers Message-ID: Hi Histoland, I would love to hear from folks who are currently using the Primera slide printers in your lab. We are working towards bringing them on line (networked) and still have not worked out the physical bugs for printing and slide jamming. We want to know some of the issues you have worked through and what your fixes have been. In fairness we are also contacting the supplier's customer service to help us on their end. Please reply to me directly, Teri From JMacDonald at mtsac.edu Wed Jan 18 12:50:16 2017 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Wed, 18 Jan 2017 10:50:16 -0800 Subject: [Histonet] California State Histo Symposium Message-ID: The California Society for Histotechnology will be hosting the 41st Annual Symposium in San Jose May 19-21 at the Hilton. We are accepting abstracts for workshops at this time. Although it is the 41st we will be celebrating our 40th. Please email me with your abstract. jmacdonaldcsh at gmail.com or jmacdonald at mtsac.edu From Linda.Margraf at cookchildrens.org Wed Jan 18 12:57:28 2017 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Wed, 18 Jan 2017 18:57:28 +0000 Subject: [Histonet] cytology and histology jobs in IL Message-ID: Here is a message I am posting for Katrina, a non-Histonet member. Please contact her (email address at the bottom)if you have questions. Thanks! ACL Laboratories, Rosemont, IL Supervisor Cytology Lab ACL Laboratories is jointly operated by Wisconsin-based Aurora Health Care and Advocate Health Care. Created via a merger of several established laboratories, ACL continues a 40-year tradition of providing a full range of lab services. We are able to draw on the resources two major health care systems which uniquely positions us to provide superior service on a wide range of clinical and analytical testing, including molecular diagnostics. We also offer 24-hour turnaround on most routine lab tests - including toxicology, environmental, and specialty tests. Our staff includes board-certified pathologists, PhD chemists and microbiologists, registered medical technologists/technicians, cytotechnologists, histotechnicians, and specialists in information technology. Highlights * Advocate Award Winning: Advocate Healthcare is voted the "Top 100 Best Places to Work" by the Chicago Tribune, multiple years in a row * Advocate Serving the Community: 12 Advocate Hospitals and over 250 sites of care. * Advocate and ACL Market Leadership: 90 board certified pathologists on staff * Employee - Centric: ACL Laboratories is the largest hospital system laboratory in the United States consisting of two central labs, one in Rosemont, IL and one in West Allis, WI. * ACL Laboratories: Located just off I-294, the Rosemont Central Laboratory offers easy access by Metra, CTA (L-train and bus) and has free parking. Description: Serve as managing technologist for the Cytology clinical laboratory section. Incumbent will be responsible for coordinating all technical, logistical, and administrative functions to ensure prompt and accurate testing and reporting of results for clinical laboratory samples within established timeframes. Qualifications required * Associate or baccalaureate degree in laboratory science. * Professional certification by one or more of the following: American Society of Clinical Pathologists, National Certification Agency for Medical Laboratory Scientists or equivalent. * Position involves moderate and/or high complexity testing as defined by CLIA '88. * Minimum five (05) years laboratory-related experience with expertise in anatomic pathology and cytology. * Excellent verbal, written, and interpersonal communication skills. * Excellent computer skills including, but not limited to word processing, spreadsheet, and e-mail use. * Thorough knowledge of statistics and ability to apply statistical rules/analysis to laboratory decision-making processes. * Previous supervisory experience highly desirable. To learn more about the position, along with submitting an application for consideration, please click on the link below to take you to the job posting: https://career4.successfactors.com/sfcareer/jobreqcareer?jobId=57566&company=advohealth&username= ACL Laboratories, Rosemont, IL **$3000 sign on bonus** Disbursement after 90 days, 1yr and 18 months of employment Histotechnician: At Advocate Health Care our Histotechnicians work closely with the clinical pathologist preparing specimens for diagnosis. If you have a demonstrated ability to produce high quality slides in a fast paced environment this may be an opportunity for you! Position: Histology Technician, Core Lab - Full-time, Part-time and various shifts with weekend rotation and emergency call. Highlights: * Advocate Award Winning: Advocate Healthcare is voted the "Top 100 Best Places to Work" by the Chicago Tribune, multiple years in a row. * Advocate Serving the Community: 12 Advocate Hospitals and 77 patient service centers. * Advocate and ACL Market Leadership: 90 board certified pathologists on staff. * Employee - Centric: ACL Laboratories is the largest hospital system laboratory in the United States consisting of two central labs, one in Rosemont, IL and one in West Allis, WI. * Clinical Laboratory Expertise: Excellence in all areas - Core lab, Histology, Molecular, Cytogenetics, Pathology, Cytology, Microbiology and Industrial Toxicology. * ACL Laboratories: Offers easy access by Metra, CTA (L-train and bus) and has free parking. Histotechnician Position Responsibilities include but are not limited to: * Identification of specimens, frozen sections and immunoperoxidase stains for diagnosis. * Producing high quality cryo, paraffin, histochemical and immunoperoxidase diagnostic slides. * Providing technical assistance to the pathologist and surgeons with rapid diagnosis of frozen tissues sections during a surgical procedure. * Performance of preventative maintenance of laboratory equipment to ensure optimal operational integrity. * Systematic maintenance of stained slides and paraffin blocks for easy retrieval and reference. Skills Required: * Provide technical expertise to process, embed, section, and stain surgical, autopsy, cytology cell block and bone marrow specimen * Demonstrates the ability to use a microtome, cryostat, tissue processor, knife sharpener, analytical balance, PH meter and vacuum oven. * Proficient with the HIS computer system Experience Required: Associate degree and 2 years' full-time experience in accredited histopathology laboratory or 4 years' full-time experience in accredited histopathology laboratory. Eligible for HT (ASCP) exam and must obtain certification within 1 year of hire. To learn more about this position email Katrina.Volbrecht at advocatehealth.com or Reggie.Allen at advocatehealth.com. From greg.dobbin at gmail.com Wed Jan 18 13:20:27 2017 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Wed, 18 Jan 2017 15:20:27 -0400 Subject: [Histonet] Releasing of Patient Tissue Message-ID: Vanessa, We strongly encourage people that make such requests to use a funeral home or crematorium to handle the tissue for them (for example an aborted fetus; or some cultures wish to have an amputated limb buried in their future burial plot). If they take their own amputated limb and then were to carelessly or accidentally discard it, a whole host of resources will be wasted investigating the origin of the found tissue! If they insist, we have a waiver that they must sign and then we rinse the tissue very well with running water to remove most of the residual formalin before packing it up for pick up. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From monica.aguilera at irbbarcelona.org Thu Jan 19 10:52:46 2017 From: monica.aguilera at irbbarcelona.org (Monica Aguilera) Date: Thu, 19 Jan 2017 17:52:46 +0100 Subject: [Histonet] xenopus oocyte fixation Message-ID: Hello all, does anyone have a good protocol (if possible) for xenopuss oocyte fixation+paraffin embedding or cryopreservation+OCT embedding? Many thanks in advance! Kind Regards, M?nica -- M?nica Aguilera Pujabet, DVM, PhD Histopathology Facility Institute for Research in Biomedicine - IRB Barcelona Baldiri Reixac, 10 E-08028 Barcelona - Spain Tel: +34 934033776 <%2B34%20934020546> monica.aguilera at irbbarcelona.org From cfields at mlkch.org Thu Jan 19 11:46:42 2017 From: cfields at mlkch.org (Carol G Fields) Date: Thu, 19 Jan 2017 17:46:42 +0000 Subject: [Histonet] Bronchoscopy Procedure Message-ID: Hi Netters, Does anyone have a Bronchoscopy Procedure they could please share with me? I need the Pathology side of how the specimen is processed. I have found a few for collection by surgery but not our end of it. It would be greatly appreciated. Thank you in advance, Carole Carole Fields, HT (ASCP) Lead Histotechnologist, Pathology Laboratory Martin Luther King Jr. Community Hospital 1680 E. 120th Street Los Angeles, CA 90059 O:424-338-8000 x 8341 cfields at mlkch.org www.MLKCommunityHospital.org www.facebook.com/YourMLKCH www.twitter.com/YourMLKCH This email message and any files transmitted are sent with confidentiality in mind and contain privileged or copyright information. You must not present this message to another party without gaining permission from the sender. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. Any views expressed in this message are those of the sender, except where the sender specifically states them to be the views of Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. If you have received this message in error, please notify the sender immediately, and delete this email from your system. We do not guarantee that this material is free from viruses or any other defects although due care has been taken to minimize the risk. From Pamela.Johnson at STJUDE.ORG Thu Jan 19 12:07:08 2017 From: Pamela.Johnson at STJUDE.ORG (Johnson, Pamela) Date: Thu, 19 Jan 2017 18:07:08 +0000 Subject: [Histonet] Primera slide printers In-Reply-To: References: Message-ID: We use them at St Jude. We have upgraded the firmware a few times with assistance from Tech Support. That has helped in preventing jams. You also need to make sure you are using quality slides so they are not sticking together. We are in the South where it is very humid. We have to keep our lab very cool and thumb through the slides before loading them in the hopper. Pam Johnson, BS, HT (ASCP) Lab Manager Veterinary Pathology Core Lab St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105-3678 Lab - 901-595-2272 Office - 901-595-3355 Mobile - 901-692-6978 -----Original Message----- From: Teri Johnson [mailto:tejohnson at genoptix.com] Sent: Wednesday, January 18, 2017 12:47 PM To: 'histonet at lists.utsouthwestern.edu' Cc: Angela McIntosh Subject: [Histonet] Primera slide printers Hi Histoland, I would love to hear from folks who are currently using the Primera slide printers in your lab. We are working towards bringing them on line (networked) and still have not worked out the physical bugs for printing and slide jamming. We want to know some of the issues you have worked through and what your fixes have been. In fairness we are also contacting the supplier's customer service to help us on their end. Please reply to me directly, Teri ________________________________ Email Disclaimer: www.stjude.org/emaildisclaimer Consultation Disclaimer: www.stjude.org/consultationdisclaimer From Sandra.Etheridge at gov.bc.ca Thu Jan 19 12:33:29 2017 From: Sandra.Etheridge at gov.bc.ca (Etheridge, Sandra AGRI:EX) Date: Thu, 19 Jan 2017 18:33:29 +0000 Subject: [Histonet] ThermoFisher SlideMate AS Message-ID: <01dfac4e9c844c55a6cbfc5967c2b69a@E3PMBX04.idir.BCGOV> Hi everyone, We are looking at purchasing slide printers for each of our cutting stations. Is anyone using ThermoFisher's SlideMate AS with the touch screen and top loading slide hopper? Your feedback would be appreciated. Thanks. Sandra BC Ministry of Agriculture Plant and Animal Health Centre Histology/IHC 1767 Angus Campbell Road Abbotsford, BC V3G 2M3 # (604) 556-3120 From THoward at salud.unm.edu Thu Jan 19 13:06:47 2017 From: THoward at salud.unm.edu (Tamara A Howard) Date: Thu, 19 Jan 2017 19:06:47 +0000 Subject: [Histonet] xenopus oocyte fixation In-Reply-To: References: Message-ID: <1484852806944.7205@salud.unm.edu> Monica - The Klymkowsky lab has a trove of Xenopus protocols: http://klymkowskylab.colorado.edu/ Their wax and cryo page also has the reference to the 1989 Methods in Cell Biology paper covering Xenopus histological methods. If you can, leave the oocytes in the ovary - it is MUCH easier to handle a blob of them for embedding than single oocytes! Tamara Tamara Howard Dept. of Cell Biology & Physiology UNM-HSC ________________________________ From: Monica Aguilera Sent: Thursday, January 19, 2017 9:52 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] xenopus oocyte fixation Hello all, does anyone have a good protocol (if possible) for xenopuss oocyte fixation+paraffin embedding or cryopreservation+OCT embedding? Many thanks in advance! Kind Regards, M?nica -- M?nica Aguilera Pujabet, DVM, PhD Histopathology Facility Institute for Research in Biomedicine - IRB Barcelona Baldiri Reixac, 10 E-08028 Barcelona - Spain Tel: +34 934033776 <%2B34%20934020546> monica.aguilera at irbbarcelona.org From Richard.Cartun at hhchealth.org Thu Jan 19 13:33:59 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Thu, 19 Jan 2017 19:33:59 +0000 Subject: [Histonet] Releasing of Patient Tissue In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E953EB4B9@HHCEXCHMB03.hhcsystem.org> Our policy is to not release any specimen that comes to pathology in formalin. If the patient is adamant about getting their specimen they will have to make arrangements with a funeral home to collect the specimen, and the appropriate release must be signed. Hardware and other medical devices will be released to the appropriate company representative or shipped back to the manufacturer. I know that our "Labor & Delivery" unit will release an occasional placenta if arrangements have been made prior to the delivery (and there is a very specific procedure that has to be followed). But, once again, if the specimen is in formalin we cannot release it to the patient or their family. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Vanessa Keeton via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, January 18, 2017 10:31 AM To: Ace Subject: [Histonet] Releasing of Patient Tissue Good Morning All! I was wondering what everyone's policies were on releasing of patient tissue other than stones, placentas, and hardware. Normally we only release stones, placentas, and hardware but have been receiving request for other types of tissues lately and was just curious as to what the norm seems to be pertaining to this issue and if you do release the tissue, how do you limit patient exposure to blood, body fluids and chemicals? Thanks!!! Vanessa Keeton _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From gu.lang at gmx.at Fri Jan 20 08:28:39 2017 From: gu.lang at gmx.at (Gudrun Lang) Date: Fri, 20 Jan 2017 15:28:39 +0100 Subject: [Histonet] staining samples years in formalin In-Reply-To: References: Message-ID: <002a01d27329$7fbb4b40$7f31e1c0$@gmx.at> Hi Mary Lou, I think the problem is, that proteoglycanes will be solved by acids through decal. Try EDTA decal or prolong the staining time in Alcianblue - maybe for 2 hours? Try to decal your controls in the same manner and compare the results. I don't think, that formalin fixation is the culprit, but long storage in aequous solutions like formalin may influence watersoluble proteoglycans. Gudrun -----Urspr?ngliche Nachricht----- Von: Mary Lou Norman via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 18. Januar 2017 15:01 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] staining samples years in formalin Dear Histonetters, My samples have been in formalin up to 3 years and there have been complaints about poor staining. Please help me with solutions if there are any. My controls have not been in formalin as long. The complaint has been with the Alcian Blue pH2.5 and Saf O specifically. The samples are stifles so I need to decal them also. I use formic/na citrate. Any and all comments, suggestions are much appreciated. Thank you. Mary Lou Norman _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brannon at alliedsearchpartners.com Fri Jan 20 13:14:04 2017 From: brannon at alliedsearchpartners.com (Brannon Owens) Date: Fri, 20 Jan 2017 19:14:04 +0000 Subject: [Histonet] Histology Grossing Technician (3rd shift) job opening in Dallas, TX Message-ID: Part grossing part general histology, full time job opportunity! Send an updated resume to brannon at alliedsearchpartners.com or give me a call to discuss. Check out our article in I4Biz June's Addition "Allied Search Partners connecting Labs to Employees" http://www.i4biz.com/sales-marketing/allied-search-partners-connects-labs-to-employees/ To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers/ Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 Direct Line: 407.413.9421 F: 888.388.7572 Jobs at Allied [Join Allied Search Partners Talent Network] From pdefazio802 at gmail.com Fri Jan 20 13:28:02 2017 From: pdefazio802 at gmail.com (Pam DeFazio) Date: Fri, 20 Jan 2017 19:28:02 +0000 Subject: [Histonet] Powdered gloves Message-ID: We just found out the powdered non-sterile gloves our pathologists use for grossing are discontinued per FDA ban. I understand this for exam/ surgical gloves. Anyone know where we might get some. Again powdered, non-sterile. Thanks for your help! Pam From Melissa.Kuhnla at chsli.org Mon Jan 23 09:54:01 2017 From: Melissa.Kuhnla at chsli.org (Kuhnla, Melissa) Date: Mon, 23 Jan 2017 15:54:01 +0000 Subject: [Histonet] ventana customers Message-ID: <4F2CE306C672504AAE5243945B1B4CD7184C4211@MVDCVM01XCN002.chsli.org> Good Morning, I am looking for feedback into technical bulletins and memos recently released. We run benchmark ultras and special strainers. We are also a facility that uses Vantage. We have never used the clear label overlays they say are mandatory. I also do not like the sounds of the software patch they are looking to install to eliminate the rare occurrence of invalid keycodes. Any input or opinions? If you are opting out of these...how are you documenting? Thank you Melissa Kuhnla Lead Medical Technologist for IHC and FISH testing Regional Laboratory Services Good Samaritan Hospital 631-609-2551 The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From tejohnson at genoptix.com Mon Jan 23 14:05:59 2017 From: tejohnson at genoptix.com (Teri Johnson) Date: Mon, 23 Jan 2017 20:05:59 +0000 Subject: [Histonet] ventana customers Message-ID: Melissa wrote: Good Morning, I am looking for feedback into technical bulletins and memos recently released. We run benchmark ultras and special strainers. We are also a facility that uses Vantage. We have never used the clear label overlays they say are mandatory. I also do not like the sounds of the software patch they are looking to install to eliminate the rare occurrence of invalid keycodes. Any input or opinions? If you are opting out of these...how are you documenting? Thank you Melissa Kuhnla Lead Medical Technologist for IHC and FISH testing Regional Laboratory Services Good Samaritan Hospital 631-609-2551 Hi Melissa, Why wouldn't you use the tools for their platforms that they provide? Using the clear label overlays are taught in the training they provide after you purchase the instrumentation. The overlays were provided to address problems with heat and chemicals affecting them with earlier label versions. By protecting the printing you keep the integrity of the information contained there intact. In addition, the little label ramp is designed to assure proper dispersion of the solutions on the system. We recently allowed them to install the patches on our instruments. Again, that is like using a Windows product and rejecting a software update that could potentially address or fix a security issue. Why wouldn't you want assurance that they are fixing something that needs it? I'd much rather be assured that the instrument is delivering the proper stain protocol every time I do a staining run. Best wishes, Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA? 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com From tkngflght at yahoo.com Mon Jan 23 15:54:46 2017 From: tkngflght at yahoo.com (Cheryl) Date: Mon, 23 Jan 2017 21:54:46 +0000 (UTC) Subject: [Histonet] Small private lab seeking histotech with gross qualifications - Wyoming References: <1055760333.2103376.1485208486478.ref@mail.yahoo.com> Message-ID: <1055760333.2103376.1485208486478@mail.yahoo.com> Hi Guys- Looking for a full time permanent histotech to man a solo lab. ?New equipment, reasonable workload. ?Coverage for vacation days is arranged.? Give me a shout and I'll share the details! Cheryl?Cheryl Kerry, HT(ASCP) Full Staff Inc. ? admin at fullstaff.org?800.756.3309 Phone & Fax https://www.facebook.com/TheHistologyCompany/ From tkngflght at yahoo.com Mon Jan 23 15:59:50 2017 From: tkngflght at yahoo.com (Cheryl) Date: Mon, 23 Jan 2017 21:59:50 +0000 (UTC) Subject: [Histonet] HT with grossing credentials - PRN and FT needed References: <2031402365.2133653.1485208790456.ref@mail.yahoo.com> Message-ID: <2031402365.2133653.1485208790456@mail.yahoo.com> This is for the lab I currently work in -- it's growing fast and we need help! Routine biopsy work from grossing through H&E with special stains and potentially IHC. ? We're building a new lab with a move-in date in March. ? It's gonna be beautiful! If you're curious-- shoot me an email/resume with a best time to call and I'll reach out: ? ? ? ? ckerry at adgpath.com PLEASE feel free to share with friends and colleagues. ?This is a sweetheart job-- quality first, great people and a really nice corporate culture --? Cheryl?Cheryl Kerry, HT(ASCP) Full Staff Inc. ? admin at fullstaff.org?800.756.3309 Phone & Fax https://www.facebook.com/TheHistologyCompany/ From ADuddey at firsthealth.org Tue Jan 24 07:53:45 2017 From: ADuddey at firsthealth.org (Duddey, Aimee) Date: Tue, 24 Jan 2017 13:53:45 +0000 Subject: [Histonet] ventana customers In-Reply-To: <4F2CE306C672504AAE5243945B1B4CD7184C4211@MVDCVM01XCN002.chsli.org> References: <4F2CE306C672504AAE5243945B1B4CD7184C4211@MVDCVM01XCN002.chsli.org> Message-ID: <09FBA01CA9B6374A83C5C76E09E46188A3EE0565@EXMAIL1-FHC.firsthealth.org> I am glad to see someone else is concerned about the upgrade. The chance of an entire run aborting is a bit frightening, mainly for the loss of irreplaceable small biopsy tissue. The possibility of an incorrectly stained slide is equally as frightening. However, with appropriate controls an incorrect antibody should be detectable. I don't believe that either option is good, but the only ones to work with. The overlay on the other hand is something that we have never used. When we first started with Vantage (we were early adopters) the overlay was not available. When it was we tried it for a while and the only difference was that it gummed up the coverslipper if slides were in xylene too long before they were coverslipped. We are still contemplating the upgrade as the resolution is tentative for early summer. Aimee M. Duddey, MLT(ASCP) Assistant Director of Laboratory - Pathology FH Moore Regional Hospital 910-715-5286 office 910715-1944 fax -----Original Message----- From: Kuhnla, Melissa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, January 23, 2017 10:54 AM To: ' (histonet at lists.utsouthwestern.edu)' Subject: [Histonet] ventana customers Good Morning, I am looking for feedback into technical bulletins and memos recently released. We run benchmark ultras and special strainers. We are also a facility that uses Vantage. We have never used the clear label overlays they say are mandatory. I also do not like the sounds of the software patch they are looking to install to eliminate the rare occurrence of invalid keycodes. Any input or opinions? If you are opting out of these...how are you documenting? Thank you Melissa Kuhnla Lead Medical Technologist for IHC and FISH testing Regional Laboratory Services Good Samaritan Hospital 631-609-2551 The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From allanvv at gmail.com Tue Jan 24 10:27:08 2017 From: allanvv at gmail.com (Allan Wang) Date: Tue, 24 Jan 2017 11:27:08 -0500 Subject: [Histonet] ventana customers In-Reply-To: <09FBA01CA9B6374A83C5C76E09E46188A3EE0565@EXMAIL1-FHC.firsthealth.org> References: <4F2CE306C672504AAE5243945B1B4CD7184C4211@MVDCVM01XCN002.chsli.org> <09FBA01CA9B6374A83C5C76E09E46188A3EE0565@EXMAIL1-FHC.firsthealth.org> Message-ID: I've found that using Clear Rite 3 instead of xylene prevented the stickers from peeling at the bottom and producing that sticky residue. But I also have the liquid level low to prevent direct contact. Allan Wang On Tue, Jan 24, 2017 at 8:53 AM, Duddey, Aimee via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I am glad to see someone else is concerned about the upgrade. The chance > of an entire run aborting is a bit frightening, mainly for the loss of > irreplaceable small biopsy tissue. The possibility of an incorrectly > stained slide is equally as frightening. However, with appropriate > controls an incorrect antibody should be detectable. I don't believe that > either option is good, but the only ones to work with. The overlay on the > other hand is something that we have never used. When we first started > with Vantage (we were early adopters) the overlay was not available. When > it was we tried it for a while and the only difference was that it gummed > up the coverslipper if slides were in xylene too long before they were > coverslipped. We are still contemplating the upgrade as the resolution is > tentative for early summer. > > Aimee M. Duddey, MLT(ASCP) > Assistant Director of Laboratory - Pathology > FH Moore Regional Hospital > 910-715-5286 office > 910715-1944 fax > > > > -----Original Message----- > From: Kuhnla, Melissa via Histonet [mailto:histonet at lists. > utsouthwestern.edu] > Sent: Monday, January 23, 2017 10:54 AM > To: ' (histonet at lists.utsouthwestern.edu)' > Subject: [Histonet] ventana customers > > Good Morning, I am looking for feedback into technical bulletins and memos > recently released. We run benchmark ultras and special strainers. We are > also a facility that uses Vantage. We have never used the clear label > overlays they say are mandatory. I also do not like the sounds of the > software patch they are looking to install to eliminate the rare occurrence > of invalid keycodes. Any input or opinions? If you are opting out of > these...how are you documenting? Thank you > > Melissa Kuhnla > Lead Medical Technologist > for IHC and FISH testing > Regional Laboratory Services > Good Samaritan Hospital > 631-609-2551 > > The information in this e-mail, and any attachments therein, is > confidential and for use by the intended addressee only. If this message is > received by you in error please do not disseminate or read further. Please > reply to the sender that you have received the message in error, then > delete the message. Although Catholic Health Services of Long Island > attempts to sweep e-mail and attachments for viruses, it does not guarantee > that either are virus-free and accepts no liability for any damage > sustained as a result of viruses. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Luis.Chiriboga at nyumc.org Tue Jan 24 16:00:02 2017 From: Luis.Chiriboga at nyumc.org (Chiriboga, Luis) Date: Tue, 24 Jan 2017 22:00:02 +0000 Subject: [Histonet] PT LInk Message-ID: <3E6798F00C9F494399E96B720ECD14292DD5CB24@MSGWBDCPMBX32.nyumc.org> Just obtained a standalone PT link and hoping someone has a digital copy of the manual they could share? Luis Chirboga NYULMC ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= From akemiat3377 at gmail.com Thu Jan 26 22:18:35 2017 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Thu, 26 Jan 2017 20:18:35 -0800 Subject: [Histonet] RIP Wanda Platt Jones Message-ID: <8F820193-D0C1-412D-BA58-AB922721E5EE@gmail.com> My feelings for Wanda Platt Jones: Although I am Buddhist, and believe in reincarnation, I am untuned to all religions! In Wanda's case, perhaps, she was needed to be with her GOD to help serve in the best way possible for the ultimate good of everyone. She had an energy and presence which was focused unconditionally for true GOOD and LOVE for everyone. Wanda to me is a True Angel; who now is looking down on us, and helping us to become better human beings. Anyone she came in contact with benefitted from her remarkable love, sweetness, and acceptance. I will truly miss her, but I know she has not left us. She is hugging and guiding us, and showering us with her love and sweetness. She is sitting in a Prestigious Histology Chair in the Sky with all the Greats, such as the likes of Chuck Churukian. He was another Great Christian Histologist, and a Very, Dear Friend! XoXo From Royl1 at LabCorp.com Fri Jan 27 06:01:24 2017 From: Royl1 at LabCorp.com (Roy, Lisa) Date: Fri, 27 Jan 2017 12:01:24 +0000 Subject: [Histonet] CA 15-3 and CA 27.29 Message-ID: I was wondering if anyone out there knows of a lab that is performing CA 15-3 and/or CA 27.29 by IHC on FFPE tissue? Thanks in advance. Lisa Roy, HT(ASCP)cm Histology Supervisor Worcester, MA -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. From Blanca.Lopez at UTSouthwestern.edu Fri Jan 27 08:41:11 2017 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Fri, 27 Jan 2017 14:41:11 +0000 Subject: [Histonet] Tissues storage question Message-ID: <1e997c06b1b8479694576e58e55d3006@SWMS13MAIL12.swmed.org> Dear Histonets, I would like to ask everybody what do you think about a specific way to storage tissues in -80 C for long time of periods and how this type of procedure can affect those tissues. This is my first time that I heard about this procedure: I have a customer that storage hundreds of tissues this way, she fixed them in 4 paraformaldehyde for long time then transfer to a 100% alcohol and storage them in -80 C for several years...Can you told me if this procedure is wrong or good and how can affect those tissues? I told her that I can test a couple to see how they react...I process them starting 70% alcohol for 12 hrs. processing, cut them (they were little crunchy) and perform an H&E. everything stain pink except the nucleus were fade purple. It looks weird to me. My question is how can she troubleshooting those tissues? Can we fix them in formalin again and start a new process? Can we wash the tissues in soap water to clean them? How can we do to make those tissue back to normal? Is alcohol a bad storage reagent? I am kind of loss with this case. I really need to understand how the tissue get affected to be storage this way. How can I troubleshooting? Thanks for your advices:) Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From mward at wakehealth.edu Fri Jan 27 09:01:11 2017 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Fri, 27 Jan 2017 15:01:11 +0000 Subject: [Histonet] Merkel cell polyomavirus antibody Message-ID: Good morning, I have had a request for this antibody but I am having trouble locating a vendor for it. If anyone has any information on vendor, etc. , I would appreciate it. We have Leica Bond 3s. Thank you in advance for your help. Martha Ward Wake Forest Baptist Health From cforster at umn.edu Fri Jan 27 10:21:05 2017 From: cforster at umn.edu (Colleen Forster) Date: Fri, 27 Jan 2017 10:21:05 -0600 Subject: [Histonet] Tissues storage question In-Reply-To: <1e997c06b1b8479694576e58e55d3006@SWMS13MAIL12.swmed.org> References: <1e997c06b1b8479694576e58e55d3006@SWMS13MAIL12.swmed.org> Message-ID: Greetings Blanca, Let me think about this for a bit....I will get back to you soon! Respectfully, Colleen FOrster HT(ASCP)QIHC BioNet Histology and Research Laboratory 612-626-1930 On Fri, Jan 27, 2017 at 8:41 AM, Blanca Lopez via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Dear Histonets, > I would like to ask everybody what do you think about a specific way to > storage tissues in -80 C for long time of periods and how this type of > procedure can affect those tissues. > This is my first time that I heard about this procedure: > I have a customer that storage hundreds of tissues this way, she fixed > them in 4 paraformaldehyde for long time then transfer to a 100% alcohol > and storage them in -80 C for several years...Can you told me if this > procedure is wrong or good and how can affect those tissues? > I told her that I can test a couple to see how they > react...I process them starting 70% alcohol for 12 hrs. processing, cut > them (they were little crunchy) and perform an H&E. everything stain pink > except the nucleus were fade purple. It looks weird to me. > > My question is how can she troubleshooting those tissues? Can we fix them > in formalin again and start a new process? Can we wash the tissues in soap > water to clean them? How can we do to make those tissue back to normal? Is > alcohol a bad storage reagent? > > I am kind of loss with this case. I really need to understand how the > tissue get affected to be storage this way. How can I troubleshooting? > > Thanks for your advices:) > > Blanca Lopez > Histotech (ASCP) > UTSW Tissue Resource K1.210 > Simmons Comprehensive Cancer Center > UT Southwestern Medical Center > Telephone: 214-648-7598 > Email: Blanca.Lopez at utsouthwestern.edu > > > ________________________________ > > UT Southwestern > > > Medical Center > > > > The future of medicine, today. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bliven.laura at marshfieldclinic.org Fri Jan 27 12:48:23 2017 From: bliven.laura at marshfieldclinic.org (Bliven, Laura M) Date: Fri, 27 Jan 2017 18:48:23 +0000 Subject: [Histonet] HSV Slides needed for Validation Message-ID: Looking for a commercial or lab source for IHC unstained slides that we can use for validation of HSV type I & type II please email me at bliven.laura at marshfieldclinic.org. Thanks, Laura ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From criley at dpspa.com Mon Jan 30 06:57:02 2017 From: criley at dpspa.com (Charles Riley) Date: Mon, 30 Jan 2017 07:57:02 -0500 Subject: [Histonet] Breast specimens Message-ID: What is the best way to handle Breast specimens that were grossed too thick and did not process well? Our medical director does not want us to reprocess the tissue but it is almost impossible to get even a remotely decent section. If anyone has any other tips please let me know as soon as possible -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From tabbott at pennstatehealth.psu.edu Mon Jan 30 07:37:29 2017 From: tabbott at pennstatehealth.psu.edu (Abbott, Tanya) Date: Mon, 30 Jan 2017 13:37:29 +0000 Subject: [Histonet] TempTrak monitoring system Message-ID: <103458cc246d4defab49e4a6a36f49d1@EXCHSRVR12.mshmc.local> Good morning! Is anyone using the TempTrak automated temperature monitoring system in their Lab? We are getting ready to go live with it, but are having problems with TempTrak probes reading the proper temperatures for our cryostats. And also a -30C refrigerator in another part of the Lab. The system doesn't seem to like cold temps! Any input would be greatly appreciated! Tanya Tanya G. Abbott Pathology Manager PennState Health St. Joseph Reading Pennsylvania tabbott at pennstatehealth.psu.edu 610-378-2635 From j.benavides at eae.csic.es Mon Jan 30 09:58:50 2017 From: j.benavides at eae.csic.es (Julio Benavides) Date: Mon, 30 Jan 2017 16:58:50 +0100 Subject: [Histonet] Leica DM2000 microscope + integrated camera In-Reply-To: References: Message-ID: <0dd76ad6-ca0e-f791-bdbf-7e5e51f0ed75@eae.csic.es> Hi there, We are considering buying a leica DM 2000 microscope with an integrated camera ICC50W. Anyone has any experience with this microscope? is the camera any good (thinking mainly on discussion sessions with students but also your odd good quality picture for publication). Your thoughts would be most appreciated! Thanks a lot Cheers Julio From jpiche at wtbyhosp.org Mon Jan 30 11:16:44 2017 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Mon, 30 Jan 2017 17:16:44 +0000 Subject: [Histonet] CAP Gen 41770 glassware cleaning Message-ID: <631955447A364B45B9458D290563511001077AB947@WIN08-MBX-01.wtbyhosp.org> Hi Everyone, I was wondering if anyone would mind sharing their procedure for glassware cleaning? We used to send our glassware down to Central Sterile supply but we have been bleaching our glassware/plastics and have had no issues with special stains. Is it a requirement to use detergent and then test for it? Thank you in advance and have a great day! Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From angie at ka-recruiting.com Mon Jan 30 12:50:37 2017 From: angie at ka-recruiting.com (Angie Laparidis) Date: Mon, 30 Jan 2017 13:50:37 -0500 Subject: [Histonet] Little Rock Day Shift Histotech Recruitment Message-ID: Happy Monday Histonet Users! I wanted to share a brand new opening with a client of mine in Little Rock, AR. It is a hospital setting-with the shifts starting from 4:30 to 6:00 AM and are eight hours long. Strong preference for surgpath experience. Great hospital, great location, amazing shift- this position will not be open very long! Send your applications to me at angie at ka-recruiting.com and I look forward to speaking with you! Sincerely, *Angie Laparidis*Healthcare Recruiter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South, Boston, MA, 02109 W: 617.746-2744 (*please note this is a new number*) F: (617) 507-8009 Angie at ka-recruiting.com Our openings are updated daily at www.ka-recruiting.com From SteveM at mcclainlab.com Mon Jan 30 14:14:24 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Mon, 30 Jan 2017 20:14:24 +0000 Subject: [Histonet] Histonet Digest, Vol 158, Issue 22 Breast Specimen Message-ID: In my experience, rushing to process fatty or inadequately fixed specimens is a fool's game. In my opinion, this problem cannot be solved by the histotechs- it begins with the grossers and is one for the pathologists to solve at the grossing bench. Suggestion #1 Do nothing and let the medical director pathologist/ sign it out/deal w this individual case. Suggestion #2 Sometimes a decent section can be obtained after changing paraffin. [place the blocks in molds and melt the blocks and change to new paraffin- let them sit in the embedding center in the new paraffin for 60 minutes. Re-embed in new paraffin (2 changes) and then re-embed.] Suggestion #3 Reprocess these blocks if permitted, recognizing that breast markers (if cancerous) may be erroneous. Suggestion #4 Seek to prevent future occurrences by adjusting behavior at the grossing bench. a) first ensuring adequate fixation and b) second ensuring adequate length/time of processing. Steve A. McClain, MD 631 361 4000 What is the best way to handle Breast specimens that were grossed too thick and did not process well? Our medical director does not want us to reprocess the tissue but it is almost impossible to get even a remotely decent section. If anyone has any other tips please let me know as soon as possible -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs********************** From sandra.cheasty at wisc.edu Mon Jan 30 15:00:59 2017 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Mon, 30 Jan 2017 21:00:59 +0000 Subject: [Histonet] Syringe for Lab Vision 720D Stainer Needed! Message-ID: Hello everyone! Can someone tell me where I can get a replacement syringe for the Lab Vision 720 stainer? It is supposed to be part # NM014. Thermo Fisher has been unable to help me so far. Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From rsrichmond at gmail.com Mon Jan 30 15:46:22 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Mon, 30 Jan 2017 16:46:22 -0500 Subject: [Histonet] Breast specimens Message-ID: Charles Riley HT(ASCP)CM asks: >>What is the best way to handle breast specimens that were grossed too thick and did not process well? Our medical director does not want us to reprocess the tissue but it is almost impossible to get even a remotely decent section. If anyone has any other tips please let me know as soon as possible.<< This 78 year old pathologist says, no sympathy, make your pathologists cut 'em right in the first place. I agree reprocessing is undesirable, but sometimes there's no other way to get a section. I'm not sure I've ever had to have a case of my own reprocessed, but I sure have with cases I've inherited from a departing pathologist. Show your pathologists their miserable blocks if they don't agree. Bob Richmond Samurai Pathologist Maryville TN From wbenton at cua.md Tue Jan 31 06:44:32 2017 From: wbenton at cua.md (Walter Benton) Date: Tue, 31 Jan 2017 12:44:32 +0000 Subject: [Histonet] Syringe for Lab Vision 720D Stainer Needed! In-Reply-To: References: Message-ID: <06e70a2e9b1e424fa453f9bc233d8118@MAIL01.GCU-MD.local> Check Biocare Medical. They use the same "box" and just place their name on it. Biocare calls it the Nemesis. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: Sandra Cheasty via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, January 30, 2017 4:01 PM To: Histonet (histonet at lists.utsouthwestern.edu) Subject: [Histonet] Syringe for Lab Vision 720D Stainer Needed! Hello everyone! Can someone tell me where I can get a replacement syringe for the Lab Vision 720 stainer? It is supposed to be part # NM014. Thermo Fisher has been unable to help me so far. Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From Liz.Drinkall at nmhs.org Tue Jan 31 08:47:47 2017 From: Liz.Drinkall at nmhs.org (Drinkall, Liz) Date: Tue, 31 Jan 2017 14:47:47 +0000 Subject: [Histonet] Breast specimens Message-ID: For breast specimens that didn't process well, you could try melting them down and placing them in warm paraffin for 1-2 hours. If you're concerned that there could still be xylene in the tissue, you could try to squeeze out some of the excess xylene. Re-embed the blocks and try cutting again. We have been able to avoid reprocessing in the past by doing this. Liz Liz Drinkall,?MS, HTL (ASCP) CM Liz.Drinkall at nmhs.org Methodist Hospital Histology Lab/Nebraska Collaborative Lab 8303 Dodge St. Omaha, NE 68114 (402)354-4572/(402)354-7974 -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Monday, January 30, 2017 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 158, Issue 22 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Breast specimens (Charles Riley) 2. TempTrak monitoring system (Abbott, Tanya) 3. Leica DM2000 microscope + integrated camera (Julio Benavides) 4. CAP Gen 41770 glassware cleaning (Piche, Jessica) ---------------------------------------------------------------------- Message: 1 Date: Mon, 30 Jan 2017 07:57:02 -0500 From: Charles Riley To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Breast specimens Message-ID: Content-Type: text/plain; charset=UTF-8 What is the best way to handle Breast specimens that were grossed too thick and did not process well? Our medical director does not want us to reprocess the tissue but it is almost impossible to get even a remotely decent section. If anyone has any other tips please let me know as soon as possible -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs This message and any included attachments are from Nebraska Methodist Health System and its affiliates and are intended only for the addressee. The message may contain privileged, confidential and/or proprietary information intended only for the person(s) named. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Nebraska Methodist Health System and its affiliates in Omaha, Nebraska, U.S.A at (402)354-2280. From Kathleen.Caleri at RoswellPark.org Tue Jan 31 10:58:21 2017 From: Kathleen.Caleri at RoswellPark.org (Caleri, Kathleen) Date: Tue, 31 Jan 2017 16:58:21 +0000 Subject: [Histonet] job descriptions Message-ID: We are currently reviewing job descriptions...so, what tasks do you require of your histotechs? Besides the basic embedding, cutting, and staining (routine and special IHC), instrument maintenance...what else are they expected to do-what do you consider to be appropriate tasks for entry level techs and for seasoned techs? Do they do any grossing-and to what extent? Do they assist with autopsies-to what extent? If anyone wants to share their descriptions, please email me directly at Kathleen.caleri at roswellpark.org I am mostly interested in what NYS labs are doing but would like to hear from everyone. Thanks! Kate Caleri BS HT(ASCP) Histology Lab Supervisor Roswell Park Cancer Institute This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From mtoole at dcol.net Tue Jan 31 11:37:39 2017 From: mtoole at dcol.net (Mike Toole) Date: Tue, 31 Jan 2017 11:37:39 -0600 Subject: [Histonet] CAP Gen 41770 glassware cleaning Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB34B348A9529@mail> Jessica, I am not sure about requirements to use and test for detergent, but the following steps are good practices. Clearing containers should be kept separate from other containers. They need only to be wiped out with a paper towel. No water, detergent or bleach. Alcohols can be cleaned with tap water and a brush only, then given a DI rinse to avoid residue. Wipe dry. Stain containers should also be cleaned only with a brush and tap water. If necessary, a small amount of a 10% solution of HCl and 70% OH can be used followed by a thorough DI rinsing. If this is not sufficient, then follow with 10% bleach being sure to rinse thoroughly with DI water and wipe dry. Chlorine test strips and phosphate test kits can be used to test for bleach and detergent residues. Even though detergent is not called for. Chlorine test strip: https://www.indigo.com/test_strips/disinfectants_sanitizers/chlorine_and_iodine/chlorine-test-papers-200ppm-33815.html API Phosphate Test Kit: http://www.apifishcare.com/product.php?id=589#.WIEFOlMrIkI Mike From jford at cytomx.com Tue Jan 31 13:35:47 2017 From: jford at cytomx.com (Judi Ford) Date: Tue, 31 Jan 2017 19:35:47 +0000 Subject: [Histonet] Sample accessioning software Message-ID: Hi Everyone, Happy Tuesday :). My group is looking into software which would assign accession numbers, track histology specimens, list archive location, track disposition info, hold images, ability to attach scientific data, has security and to create reports. I would love to hear what anyone may be using or can suggest that I look into. Thanks so much for any and all responses that come my way. I really appreciate it. Best to all, Judi Ford CytomX Therapeutics, South San Francisco, CA STATEMENT OF CONFIDENTIALITY: The information contained in this electronic message and any attachments to this message are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not an intended recipient, or a person responsible for delivering the e-mail to an intended recipient, please be advised that you have received this message in error and that any use, dissemination, forwarding, printing, or copying is strictly prohibited. Please notify the sender at CytomX Therapeutics, Inc., immediately and destroy all copies of this message and any attachments. CytomX Therapeutics, Inc. From Toni.Rathborne at RWJBH.org Tue Jan 31 14:15:04 2017 From: Toni.Rathborne at RWJBH.org (Rathborne, Toni) Date: Tue, 31 Jan 2017 20:15:04 +0000 Subject: [Histonet] Histonet Digest, Vol 158, Issue 22 Breast Specimen In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24014834D7DA@vap1013.win.rwjuh.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24014834D5FA@vap1013.win.rwjuh.edu> <59E09A4EFBD3F349BD75FDAE8AFB0F24014834D7DA@vap1013.win.rwjuh.edu> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24014834DAB6@vap1013.win.rwjuh.edu> There is also a CAP checklist standard that might help you to document these cases, without it seeming like you're complaining. Once the offenders have it brought to their attention, and know that it is being documented, they may start to give more consideration to the sections they're putting through. **NEW** 07/28/2015 ANP.10038 Tissue Sample Quality Phase II There is a procedure that describes the process by which histotechnologists provide feedback to submitting pathologists and pathology assistants on the quality of the gross tissue sections received for tissue processing. NOTE: Inadequate fixation, overly thick tissue sections, non-decalcified bone, the presence of staples, etc., can lead to poor quality histologic sections and/or poor quality special stains/special studies. This requirement applies to both laboratories that gross tissue and perform all processing onsite, as well as laboratories that gross tissue and send it to another laboratory for processing, embedding, and sectioning (regardless of the outside laboratory's accrediting organization). Records of such feedback and corrective action taken when problems are identified may be incorporated into the laboratory's quality management program. Evidence of Compliance: ? Records of feedback and corrective action for problems identified with tissue quality Toni -----Original Message----- From: Steve McClain via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, January 30, 2017 3:14 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 158, Issue 22 Breast Specimen *** This is an External Email *** In my experience, rushing to process fatty or inadequately fixed specimens is a fool's game. In my opinion, this problem cannot be solved by the histotechs- it begins with the grossers and is one for the pathologists to solve at the grossing bench. Suggestion #1 Do nothing and let the medical director pathologist/ sign it out/deal w this individual case. Suggestion #2 Sometimes a decent section can be obtained after changing paraffin. [place the blocks in molds and melt the blocks and change to new paraffin- let them sit in the embedding center in the new paraffin for 60 minutes. Re-embed in new paraffin (2 changes) and then re-embed.] Suggestion #3 Reprocess these blocks if permitted, recognizing that breast markers (if cancerous) may be erroneous. Suggestion #4 Seek to prevent future occurrences by adjusting behavior at the grossing bench. a) first ensuring adequate fixation and b) second ensuring adequate length/time of processing. Steve A. McClain, MD 631 361 4000 What is the best way to handle Breast specimens that were grossed too thick and did not process well? Our medical director does not want us to reprocess the tissue but it is almost impossible to get even a remotely decent section. If anyone has any other tips please let me know as soon as possible -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs********************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jbEFtbucNUvdEcthkK3pdmU&m=wZNGFpePMoWzTu96NU9dUZNyVdC2xYSRzzZ1L_ktQPY&s=vEUg2Xt0MzBIxlRx8k4EBLnzwfuBnWzD3K-1QN2zQyo&e= From ranna0726 at gmail.com Tue Jan 31 15:56:33 2017 From: ranna0726 at gmail.com (Ranna Mehta) Date: Tue, 31 Jan 2017 15:56:33 -0600 Subject: [Histonet] Syringe for Lab Vision 720D Stainer Needed! (Walter Benton) Message-ID: Hi Sandra, Contact Victor Wong Cell 646-378-9222 service at autostainertech.com He is very good with lab vision platform. Thanks Ranna Mehta On Tue, Jan 31, 2017 at 12:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Little Rock Day Shift Histotech Recruitment (Angie Laparidis) > 2. Re: Histonet Digest, Vol 158, Issue 22 Breast Specimen > (Steve McClain) > 3. Syringe for Lab Vision 720D Stainer Needed! (Sandra Cheasty) > 4. Re: Breast specimens (Bob Richmond) > 5. Re: Syringe for Lab Vision 720D Stainer Needed! (Walter Benton) > 6. Re: Breast specimens (Drinkall, Liz) > 7. job descriptions (Caleri, Kathleen) > 8. CAP Gen 41770 glassware cleaning (Mike Toole) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 30 Jan 2017 13:50:37 -0500 > From: Angie Laparidis > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Little Rock Day Shift Histotech Recruitment > Message-ID: > mail.gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Happy Monday Histonet Users! > > I wanted to share a brand new opening with a client of mine in Little Rock, > AR. It is a hospital setting-with the shifts starting from 4:30 to 6:00 AM > and are eight hours long. Strong preference for surgpath experience. > > > Great hospital, great location, amazing shift- this position will not be > open very long! Send your applications to me at angie at ka-recruiting.com > and > I look forward to speaking with you! > > > Sincerely, > > > *Angie Laparidis*Healthcare Recruiter > K.A. Recruiting, Inc. > 10 Post Office Square, 8th Floor South, Boston, MA, 02109 > W: 617.746-2744 (*please note this is a new number*) > F: (617) 507-8009 > Angie at ka-recruiting.com > > > Our openings are updated daily at www.ka-recruiting.com > > > ------------------------------ > > Message: 2 > Date: Mon, 30 Jan 2017 20:14:24 +0000 > From: Steve McClain > To: "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Histonet Digest, Vol 158, Issue 22 Breast > Specimen > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > In my experience, rushing to process fatty or inadequately fixed specimens > is a fool's game. > In my opinion, this problem cannot be solved by the histotechs- it begins > with the grossers and is one for the pathologists to solve at the grossing > bench. > > Suggestion #1 Do nothing and let the medical director pathologist/ sign it > out/deal w this individual case. > > Suggestion #2 Sometimes a decent section can be obtained after changing > paraffin. > [place the blocks in molds and melt the blocks and change to new paraffin- > let them sit in the embedding center in the new paraffin for 60 minutes. > Re-embed in new paraffin (2 changes) and then re-embed.] > > Suggestion #3 Reprocess these blocks if permitted, recognizing that breast > markers (if cancerous) may be erroneous. > > Suggestion #4 Seek to prevent future occurrences by adjusting behavior at > the grossing bench. > a) first ensuring adequate fixation and b) second ensuring adequate > length/time of processing. > > > Steve A. McClain, MD > 631 361 4000 > > What is the best way to handle Breast specimens that were grossed too > thick and did not process well? Our medical director does not want us to > reprocess the tissue but it is almost impossible to get even a remotely > decent section. If anyone has any other tips please let me know as soon as > possible > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs********************** > > > > ------------------------------ > > Message: 3 > Date: Mon, 30 Jan 2017 21:00:59 +0000 > From: Sandra Cheasty > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > Subject: [Histonet] Syringe for Lab Vision 720D Stainer Needed! > Message-ID: > namprd06.prod.outlook.com> > > Content-Type: text/plain; CHARSET=US-ASCII > > Hello everyone! > Can someone tell me where I can get a replacement syringe > for the Lab Vision 720 stainer? It is supposed to be part # NM014. Thermo > Fisher has been unable to help me so far. > Thanks! > Sandy > > Sandra J. Cheasty, HT (ASCP) > Histology & Necropsy Supervisor > UW-Madison, School of Veterinary Medicine > > > > ------------------------------ > > Message: 4 > Date: Mon, 30 Jan 2017 16:46:22 -0500 > From: Bob Richmond > To: "Histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Breast specimens > Message-ID: > AA at mail.gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Charles Riley HT(ASCP)CM asks: > > >>What is the best way to handle breast specimens that were grossed too > thick > and did not process well? Our medical director does not want us to > reprocess the tissue but it is almost impossible to get even a remotely > decent section. If anyone has any other tips please let me know as soon as > possible.<< > > This 78 year old pathologist says, no sympathy, make your pathologists cut > 'em right in the first place. > > I agree reprocessing is undesirable, but sometimes there's no other way to > get a section. I'm not sure I've ever had to have a case of my own > reprocessed, but I sure have with cases I've inherited from a departing > pathologist. Show your pathologists their miserable blocks if they don't > agree. > > Bob Richmond > Samurai Pathologist > Maryville TN > > > ------------------------------ > > Message: 5 > Date: Tue, 31 Jan 2017 12:44:32 +0000 > From: Walter Benton > To: Sandra Cheasty > Cc: "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Syringe for Lab Vision 720D Stainer Needed! > Message-ID: <06e70a2e9b1e424fa453f9bc233d8118 at MAIL01.GCU-MD.local> > Content-Type: text/plain; charset="iso-8859-1" > > Check Biocare Medical. They use the same "box" and just place their name > on it. Biocare calls it the Nemesis. > > > Walter Benton HT(ASCP)QIHC > Lab Operations Manager > Chesapeake Urology Associates > 806 Landmark Drive, Suite 127 > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > Chesapeakeurology.com > > Voted a Best Place to Work by > Baltimore and Modern Healthcare > Magazines. > > > > -----Original Message----- > From: Sandra Cheasty via Histonet [mailto:histonet at lists. > utsouthwestern.edu] > Sent: Monday, January 30, 2017 4:01 PM > To: Histonet (histonet at lists.utsouthwestern.edu) utsouthwestern.edu> > Subject: [Histonet] Syringe for Lab Vision 720D Stainer Needed! > > Hello everyone! > Can someone tell me where I can get a replacement syringe > for the Lab Vision 720 stainer? It is supposed to be part # NM014. Thermo > Fisher has been unable to help me so far. > Thanks! > Sandy > > Sandra J. Cheasty, HT (ASCP) > Histology & Necropsy Supervisor > UW-Madison, School of Veterinary Medicine > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > CONFIDENTIALITY NOTICE: The information contained in this electronic > message is intended solely for the personal and confidential use of the > designated recipient(s) named above and may contain information that is > protected from disclosure under applicable law. If you are not the intended > recipient, or the employee or agent responsible for delivering it to the > intended recipient, you are hereby notified that any dissemination, > distribution or copying of this transmission is strictly prohibited. If you > have received this transmission in error, please notify the transmitting > person/department immediately by email or telephone (410) 581-5881 and > delete the message without making a copy. > > > > ------------------------------ > > Message: 6 > Date: Tue, 31 Jan 2017 14:47:47 +0000 > From: "Drinkall, Liz" > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: Re: [Histonet] Breast specimens > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > For breast specimens that didn't process well, you could try melting them > down and placing them in warm paraffin for 1-2 hours. If you're concerned > that there could still be xylene in the tissue, you could try to squeeze > out some of the excess xylene. Re-embed the blocks and try cutting again. > We have been able to avoid reprocessing in the past by doing this. > Liz > > Liz Drinkall,?MS, HTL (ASCP) CM > Liz.Drinkall at nmhs.org > Methodist Hospital > Histology Lab/Nebraska Collaborative Lab > 8303 Dodge St. > Omaha, NE 68114 > (402)354-4572/(402)354-7974 > > > > -----Original Message----- > From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request@ > lists.utsouthwestern.edu] > Sent: Monday, January 30, 2017 12:00 PM > To: histonet at lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 158, Issue 22 > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific than > "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Breast specimens (Charles Riley) > 2. TempTrak monitoring system (Abbott, Tanya) > 3. Leica DM2000 microscope + integrated camera (Julio Benavides) > 4. CAP Gen 41770 glassware cleaning (Piche, Jessica) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 30 Jan 2017 07:57:02 -0500 > From: Charles Riley > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Breast specimens > Message-ID: > gmail.com> > Content-Type: text/plain; charset=UTF-8 > > What is the best way to handle Breast specimens that were grossed too > thick and did not process well? Our medical director does not want us to > reprocess the tissue but it is almost impossible to get even a remotely > decent section. If anyone has any other tips please let me know as soon as > possible > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > > > > > This message and any included attachments are from Nebraska Methodist > Health System and its affiliates and are intended only for the addressee. > The message may contain privileged, confidential and/or proprietary > information intended only for the person(s) named. Unauthorized > forwarding, printing, copying, distribution, or use of such information is > strictly prohibited and may be unlawful. If you are not the addressee, > please promptly delete this message and notify the sender of the delivery > error by e-mail or you may call Nebraska Methodist Health System and its > affiliates in Omaha, Nebraska, U.S.A at (402)354-2280. > > > > ------------------------------ > > Message: 7 > Date: Tue, 31 Jan 2017 16:58:21 +0000 > From: "Caleri, Kathleen" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] job descriptions > Message-ID: > namprd12.prod.outlook.com> > > Content-Type: text/plain; charset="us-ascii" > > > We are currently reviewing job descriptions...so, what tasks do you > require of your histotechs? Besides the basic embedding, cutting, and > staining (routine and special IHC), instrument maintenance...what else are > they expected to do-what do you consider to be appropriate tasks for entry > level techs and for seasoned techs? Do they do any grossing-and to what > extent? Do they assist with autopsies-to what extent? If anyone wants to > share their descriptions, please email me directly at > Kathleen.caleri at roswellpark.org > > I am mostly interested in what NYS labs are doing but would like to hear > from everyone. Thanks! > > > Kate Caleri BS HT(ASCP) > Histology Lab Supervisor > Roswell Park Cancer Institute > > > This email message may contain legally privileged and/or confidential > information. If you are not the intended recipient(s), or the employee or > agent responsible for the delivery of this message to the intended > recipient(s), you are hereby notified that any disclosure, copying, > distribution, or use of this email message is prohibited. If you have > received this message in error, please notify the sender immediately by > e-mail and delete this email message from your computer. Thank you. > > ------------------------------ > > Message: 8 > Date: Tue, 31 Jan 2017 11:37:39 -0600 > From: Mike Toole > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] CAP Gen 41770 glassware cleaning > Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB34B348A9529 at mail> > Content-Type: text/plain; charset="us-ascii" > > Jessica, > > > > I am not sure about requirements to use and test for detergent, but the > following steps are good practices. > > > > Clearing containers should be kept separate from other containers. They > need only to be wiped out with a paper towel. No water, detergent or bleach. > > > > Alcohols can be cleaned with tap water and a brush only, then given a DI > rinse to avoid residue. Wipe dry. > > > > Stain containers should also be cleaned only with a brush and tap water. > If necessary, a small amount of a 10% solution of HCl and 70% OH can be > used followed by a thorough DI rinsing. If this is not sufficient, then > follow with 10% bleach being sure to rinse thoroughly with DI water and > wipe dry. > > > > Chlorine test strips and phosphate test kits can be used to test for > bleach and detergent residues. Even though detergent is not called for. > > > > Chlorine test strip: https://www.indigo.com/test_strips/disinfectants_ > sanitizers/chlorine_and_iodine/chlorine-test-papers-200ppm-33815.html > > > > API Phosphate Test Kit: http://www.apifishcare.com/product.php?id=589#. > WIEFOlMrIkI > > > > Mike > > > > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 158, Issue 23 > ***************************************** > From criley at dpspa.com Tue Jan 31 18:17:18 2017 From: criley at dpspa.com (Charles Riley) Date: Tue, 31 Jan 2017 19:17:18 -0500 Subject: [Histonet] Thinprep staining Message-ID: The pathologists at my office keep asking me to fix the thin prep staining. This is not part of my expertise as I was only given about 10 minutes worth of cytoprep training in school. If anyone out there can offer any assistance for the problem it would be greatly appreciated. Here is what they have said is occurring: "The Orange G staining is too light and inconsistently distributed across the slide. I have also noticed some perinuclear artifacts such as perinuclear clearing." Thanks in advance for your help -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs