From LAdams at CVPath.org Wed Feb 1 07:22:59 2017 From: LAdams at CVPath.org (Lila Adams) Date: Wed, 1 Feb 2017 13:22:59 +0000 Subject: [Histonet] CV Path Gaithersburg,MD recruitment position Message-ID: <6f74d1ee4c804019b3dd617f4fed6e3b@Exchange01.CVPath.org> Good Morning Histotechnologist! :) I wanted to share a new opening at CV Path Gaithersburg,MD. It is a research setting near Washington DC. CV Path would prefer previous histology experience with resin-embedding techniques, but are willing to train. Please submit your resume to jobs at cvpath.org . Thanks and have a great day! Lila R. Adams IHC Research Lab CVPath Institute, Inc 19 Firstfield Road Gaithersburg, MD 20878 301-208-3570 ext 138 This message is a PRIVATE communication. This message and all attachments are a private communication and may be confidential and/or legally privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the information contained in or attached to this message is strictly prohibited. Please notify the sender of the delivery error by replying to this message, and then delete it from your system. Thank you. From mtoole at dcol.net Wed Feb 1 10:07:53 2017 From: mtoole at dcol.net (Mike Toole) Date: Wed, 1 Feb 2017 10:07:53 -0600 Subject: [Histonet] ThinPrep staining Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB34B348A9790@mail> Hi Charles, The only concern mentioned was the intensity of and distribution of the OG. OG and EA are interdependent in the pap stain and it may just be a matter of adjusting the times for OG and EA. A good book on the topic that I recommend is "Cytopreparation, Principles and Practice" by Gary Gill. It contains an entire chapter dedicated to the pap stain. https://www.amazon.com/Cytopreparation-Principles-Practice-Essentials-Cytopathology/dp/1461449324 Mike From relia1 at earthlink.net Wed Feb 1 10:26:33 2017 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 1 Feb 2017 11:26:33 -0500 Subject: [Histonet] Histotechnology Professionals Day is March 10 and I have a question for you... Message-ID: <2a7901d27ca7$f409c7b0$dc1d5710$@earthlink.net> Hi Histonetters , How are you doing today? This e-mail is a little different than the normal e-mail that I send out because I am working on a project and I need your help I do have some great jobs but first I am on the Public Relations committee for the NSH and we are doing our planning for Histotechnology Professionals Day on March 10. The help I need is this We?re looking for a few short quotes about what HPD (Histotechnology Professionals Day) or being a histotech means to you. These are going to be used during the HPD celebration on the website and Facebook pages to celebrate and inspire histotechs and promote the profession. I also have some exciting job opportunities to pass along. Please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral reward! I LOVE TO GIVE REFERRAL REWARDS!! SOME of these are RELIA exclusives MOST of these offer Sign- on Bonuses and/or Relocation Assistance ALL of these Companies offer excellent compensation, benefits and great environments. AND THEY ALL ARE READY TO HIRE!! If You Or Anyone You Know Might Be Interested In Any Of These Positions Please Contact Me. You can reach me by email at relia1 at earthlink.net Toll free at 866-607-3542 or on my cell at 407-353-5070. Here are the exciting opportunities I am talking about: Histology Supervisor- Los Angeles, CA High volume/fast pace! Histotech ? Austin, TX Awesome team great shift diff! Lead Histology Tech ? Cheyenne, WY Brand New Lab!! Dermpath Histotech ? Ft. Myers/Naples Learn Mohs! IHC Histotech ? Portland, ME Strong IHC experience preferred! Histotech ? Syracuse, NY- Day Shift New Grads Welcome! Histotech ? Kingsport, TN ? Day Shift Great Lab! Dermpath Histotech ? St. Louis, MO Exciting opportunity Mohs Histotech ? St. Louis, MO- Brand New State of the Art Lab! Histotech ? Tyler, TX State Of The Art Lab. Have a great day. I look forward to hearing back from you. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From rhayden3 at kumc.edu Wed Feb 1 13:25:46 2017 From: rhayden3 at kumc.edu (Rebecca Hayden) Date: Wed, 1 Feb 2017 19:25:46 +0000 Subject: [Histonet] IHC antibodies counts Message-ID: <94977334D8EE4D428086FE514F301E1A1C5261B9@KUMC-XCHMB201.kumc.edu> Our lab is looking to switch some of our low count IHC antibodies that are ordered to a send-out system instead of keeping low count antibodies in house. I am looking to see if other labs are doing this and what is a good number for a cut off? From jclark at pcnm.com Wed Feb 1 16:31:10 2017 From: jclark at pcnm.com (Joanne Clark) Date: Wed, 1 Feb 2017 22:31:10 +0000 Subject: [Histonet] Cholecystokinin by IHC Message-ID: Does anyone know of a commercial/reference testing lab that does Cholecystokinin by IHC? From what I can find online it looks to be an antibody that is currently only being used in research. Can any histonet pathologists shed any light on this marker for me? We have a clinician that would like to have this testing done on his patients. Thank you! Joanne Clark, BAAS, HT(ASCP)CM Director of Histology Pathology Consultants of New Mexico Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From garethdavisyuma at gmail.com Wed Feb 1 18:23:54 2017 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Wed, 1 Feb 2017 17:23:54 -0700 Subject: [Histonet] IHC Medicare policy Message-ID: <5F109CBC-593D-41DA-B121-A2DD2BD35DEE@gmail.com> Okay, I know that for Medicare cases we cannot order Special or IHC stains upfront. They can be ordered after the pathologist reads the slide. But, what are labs doing for other insurances? The lab I work in only does H. Pylori and CD 8 on GI cases. I understand cancer cases are different. I am basically interested in what labs, like our GI lab, are doing about non-Medicare cases. Are you doing Special and IHC stains, the clinical doctors order, upfront? Thanks, Gareth Davis, B.S., HT, QIHC (ASCP)cm Sent from my iPhone From KSimeone at leavittmgt.com Thu Feb 2 11:19:10 2017 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Thu, 2 Feb 2017 17:19:10 +0000 Subject: [Histonet] Lung SCC Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CC919C7DC@vm-email.leavittmgt.com> Does anyone have a few blocks of lung SCC they can spare? willing to trade! I am working up monoclonal p40 in a derm lab, any suggestions on great controls other than lung SCC (insert claims that is the best)? Thanks for any help/suggestions. Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor Delray Beach Technical Laboratory ADCS Clinics, LLC The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From Jose.R.deGuzman at gunet.georgetown.edu Fri Feb 3 12:48:08 2017 From: Jose.R.deGuzman at gunet.georgetown.edu (deGuzman, Jose R) Date: Fri, 3 Feb 2017 13:48:08 -0500 Subject: [Histonet] DAKO FLEX slides Message-ID: Hello Histonetters. Has anybody else encountered problems with the DAKO FLEX slides where paraffin sections are not adhering to the slide? J ---------------------------------------------------------------------- MedStar Health is a not-for-profit, integrated healthcare delivery system, the largest in Maryland and the Washington, D.C., region. Nationally recognized for clinical quality in heart, orthopaedics, cancer and GI. IMPORTANT: This e-mail (including any attachments) may contain information that is private, confidential, or protected by attorney-client or other privilege. If you received this e-mail in error, please delete it from your system without copying it and notify sender by reply e-mail, so that our records can be corrected... Thank you. Help conserve valuable resources - only print this email if necessary. From gu.lang at gmx.at Sun Feb 5 05:26:05 2017 From: gu.lang at gmx.at (Gudrun Lang) Date: Sun, 5 Feb 2017 12:26:05 +0100 Subject: [Histonet] eosin B solution for H&E Message-ID: <000601d27fa2$a49dcc00$edd96400$@gmx.at> Hi all! I wonder how widespread the usage of eosin B is in H&E protocols. Is there a specific difference in application to Eosin Y? just for curiosity. Gudrun From rsrichmond at gmail.com Sun Feb 5 12:48:46 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Sun, 5 Feb 2017 13:48:46 -0500 Subject: [Histonet] Eosin B solution for H&E Message-ID: Gudrun Lang (in Austria) asks: >>I wonder how widespread the usage of eosin B is in H&E protocols. Is there a specific difference in application to Eosin Y?<< Eosin B is closely related to the more commonly used eosin Y. As the letters imply, it's Bluish rather than Yellowish. I've never seen eosin B used in H&E staining protocols, but it certainly has been. Bob Richmond Samurai Pathologist Maryville, Tennessee From llewllew at shaw.ca Sun Feb 5 13:04:22 2017 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Sun, 5 Feb 2017 11:04:22 -0800 Subject: [Histonet] Eosin B solution for H&E In-Reply-To: References: Message-ID: <4bb6aeb6-ce4c-d015-fa61-5af74532403a@shaw.ca> I played around with it many years ago and found no difference except for the bluer tone. Simply substitute it for eosin Y ws gram for gram, and use the same technique. Bryan Llewellyn Bob Richmond via Histonet wrote: > Gudrun Lang (in Austria) asks: > >>> I wonder how widespread the usage of eosin B is in H&E protocols. Is > there a > specific difference in application to Eosin Y?<< > > Eosin B is closely related to the more commonly used eosin Y. As the > letters imply, it's Bluish rather than Yellowish. > > I've never seen eosin B used in H&E staining protocols, but it certainly > has been. > > Bob Richmond > Samurai Pathologist > Maryville, Tennessee > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From taylor at prometheushealthcare.com Mon Feb 6 09:16:22 2017 From: taylor at prometheushealthcare.com (Taylor Rinaldi) Date: Mon, 6 Feb 2017 10:16:22 -0500 Subject: [Histonet] Histology Recruiter here to assist you! Message-ID: <0f5f01d2808b$fa29d260$ee7d7720$@prometheushealthcare.com> Hi Histonetters! My name is Taylor Rinaldi, I'm the Recruiting Manager with Prometheus Healthcare. Does your laboratory need assistance staffing your open positions? We are top in our field at assisting hospitals and laboratories nationwide. We are able to fill all laboratory positions and have a team who specialize specifically in histology roles, both bench and supervisory. We have a database full of qualified, hardworking, and licensed Histotechs. All of whom are waiting for me to update them on the freshest and newest opportunities. Please don't hesitate to reach out if there is anything we may assist you with this year. Let's make the workplace in 2017 great, together! Thank you all in advance, Taylor Rinaldi Recruiting Manager Prometheus Healthcare Office 866-857-1434 Taylor at prometheushealthcare.com From Doug.Geddes at lhsc.on.ca Mon Feb 6 13:20:01 2017 From: Doug.Geddes at lhsc.on.ca (Doug Geddes) Date: Mon, 6 Feb 2017 19:20:01 +0000 Subject: [Histonet] PFK on frozen muscle tissue Message-ID: <5e8bf47349ae4e2c8f82e6ec835c25e1@lhsc.on.ca> Have been using D-Fructose 1,6-bisphosphate sodium salt hydrate from Sigma *(cat# F4757) in our PFK staining protocol. Have just been informed that it is discontinued, their recommended substitute is D-Fructose 1,6-bisphosphate trisodium salt hydrate (cat#F6803). Can anyone with experience know if this would work? Without us paying for it and not have it work. Many thanks , Doug This email is directed in confidence solely to the person named above and may contain confidential, privileged or personal health information. Please be aware that this email may also be released to members of the public under Ontario's Freedom of Information and Protection of Privacy Act if required. Review, distribution, or disclosure of this email by anyone other than the person(s) for whom it was originally intended is strictly prohibited. If you are not an intended recipient, please notify the sender immediately via a return email and destroy all copies of the original message. Thank you for your cooperation. From Nancy_Schmitt at pa-ucl.com Tue Feb 7 08:46:56 2017 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Tue, 7 Feb 2017 14:46:56 +0000 Subject: [Histonet] Non-gyn cytology Message-ID: Good Morning- Can you share with me how you are handling non-gyn cytology specimens? Are you working with them under a hood? In a TB room? At a counter in the histology lab that has good negative pressure? Fixed vs. unfixed? Appreciate your input- Nancy United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From kjgada at gmail.com Tue Feb 7 14:58:21 2017 From: kjgada at gmail.com (Komal Gada) Date: Tue, 7 Feb 2017 15:58:21 -0500 Subject: [Histonet] Las Vegas, NV - Partnership opportunity for student Message-ID: Hello Histo-netters, I'm reaching out to see if anyone is interested in helping with an awesome opportunity in the Las Vegas, NV area. If you are not in Vegas, please feel free to ignore / delete this message. If you are in Las Vegas, I am seeking a partnership with your lab to place a Histo student for a 2 month externship. If this is something you are willing and able to do, or if you would like to discuss the opportunity further, please contact me directly. The dates for this would be from 5/8/2017? 7/2/2017. Thanks, Komal Gada From jriggleman at globusmedical.com Wed Feb 8 12:07:24 2017 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Wed, 8 Feb 2017 18:07:24 +0000 Subject: [Histonet] Looking for a Histology Workshop Message-ID: Hi Everyone, I am looking for workshop or class for Histology/Histotechnology that lasts anywhere from 1 week to 3 months in the U.S. Preferably in-person and not online. If you have any suggestions or contacts, please let me know. Thank You, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: website: http://www.globusmedical.com | email: jriggleman at globusmedical.com An ISO 13485 Registered Company Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. Disclaimer The information contained in this communication from the sender is confidential. It is intended solely for use by the recipient and others authorized to receive it. If you are not the recipient, you are hereby notified that any disclosure, copying, distribution or taking action in relation of the contents of this information is strictly prohibited and may be unlawful. This email has been scanned for viruses and malware, and may have been automatically archived by Mimecast Ltd, an innovator in Software as a Service (SaaS) for business. Providing a safer and more useful place for your human generated data. Specializing in; Security, archiving and compliance. To find out more visit the Mimecast website. From jriggleman at globusmedical.com Wed Feb 8 15:33:47 2017 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Wed, 8 Feb 2017 21:33:47 +0000 Subject: [Histonet] Looking for a Histology Workshop In-Reply-To: References: , Message-ID: Hi Esther, We are looking for basic training to include study design, sectioning raw specimens for processing (i.e., how large should my specimen be?), processing techniques (fixation, etc.), special stains, and slide reading. This is not for HT/HTL exam preparation. We would prefer non-human tissues, as we typically work with large sheep and rabbit specimens. Only two students. Thank You, Jessica From: Esther C Peters [mailto:epeters2 at gmu.edu] Sent: Wednesday, February 08, 2017 4:18 PM To: Jessica Riggleman Cc: histonet at lists.utsouthwestern.edu Subject: Re: Looking for a Histology Workshop Is this for human or non-human tissues? How many students? Esther _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. ________________________________________ From: Jessica Riggleman Sent: Wednesday, February 8, 2017 4:12:20 PM To: Esther C Peters Cc: histonet at lists.utsouthwestern.edu Subject: RE: Looking for a Histology Workshop Hi Esther, We are looking for basic training to include study design, sectioning raw specimens for processing (i.e., how large should my specimen be?), processing techniques (fixation, etc.), special stains, and slide reading. This is not for HT/HTL exam preparation. Thank You, Jessica ________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: An ISO 13485 Registered Company website | map | email Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From: Esther C Peters [mailto:epeters2 at gmu.edu] Sent: Wednesday, February 08, 2017 3:53 PM To: Jessica Riggleman Subject: Re: Looking for a Histology Workshop Hi Jessica, It might help everyone to know exactly what you want to cover in the workshop or class. Do you need basic training, preparation for the HT/HTL exam, slide preparation, special stains, or slide reading? What is your goal? Esther Esther C. Peters, Ph.D. George Mason University Fairfax, VA 22030-4444 ________________________________________ From: Jessica Riggleman via Histonet Sent: Wednesday, February 8, 2017 1:07:24 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Looking for a Histology Workshop Hi Everyone, I am looking for workshop or class for Histology/Histotechnology that lasts anywhere from 1 week to 3 months in the U.S. Preferably in-person and not online. If you have any suggestions or contacts, please let me know. Thank You, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: website: http://www.globusmedical.com | email: jriggleman at globusmedical.com An ISO 13485 Registered Company Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. Disclaimer The information contained in this communication from the sender is confidential. It is intended solely for use by the recipient and others authorized to receive it. If you are not the recipient, you are hereby notified that any disclosure, copying, distribution or taking action in relation of the contents of this information is strictly prohibited and may be unlawful. This email has been scanned for viruses and malware, and may have been automatically archived by Mimecast Ltd, an innovator in Software as a Service (SaaS) for business. Providing a safer and more useful place for your human generated data. Specializing in; Security, archiving and compliance. To find out more visit the Mimecast website. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer The information contained in this communication from the sender is confidential. It is intended solely for use by the recipient and others authorized to receive it. If you are not the recipient, you are hereby notified that any disclosure, copying, distribution or taking action in relation of the contents of this information is strictly prohibited and may be unlawful. This email has been scanned for viruses and malware, and may have been automatically archived by Mimecast Ltd, an innovator in Software as a Service (SaaS) for business. Providing a safer and more useful place for your human generated data. Specializing in; Security, archiving and compliance. To find out more visit the Mimecast website. From histotech at dermspec.com Wed Feb 8 18:16:10 2017 From: histotech at dermspec.com (Jennifer) Date: Wed, 8 Feb 2017 16:16:10 -0800 Subject: [Histonet] hand staining immunos Message-ID: <000001d28269$b81e82f0$285b88d0$@com> Hello, My dermatopathologist wants me to start hand staining immunos for MITF. I would really like some feedback about everyone's experiences/thoughts with manually hand staining immunos and especially with MITF. Pros and cons? I am concerned about the amount of time this will take for a tech to babysit the stain and also about the consistency of the quality in the end result. Your opinions are much appreciated. Thank you! Jennifer From rjbuesa at yahoo.com Thu Feb 9 09:51:22 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 9 Feb 2017 15:51:22 +0000 (UTC) Subject: [Histonet] hand staining immunos In-Reply-To: <000001d28269$b81e82f0$285b88d0$@com> References: <000001d28269$b81e82f0$285b88d0$@com> Message-ID: <267846266.1416224.1486655482834@mail.yahoo.com> You do not have to "babysit" the procedure for it has well defined/timed steps. You just need a timer and check the slides when required. As to procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren? On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet wrote: Hello, My dermatopathologist wants me to start hand staining immunos? for MITF. I would really like some feedback about everyone's experiences/thoughts with manually hand staining immunos and especially with MITF. Pros and cons? I am concerned about the amount of time this will take for a tech to babysit the stain and also about the consistency of the quality in the end result.? Your opinions are much appreciated. Thank you! Jennifer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton at cua.md Thu Feb 9 10:19:00 2017 From: wbenton at cua.md (Walter Benton) Date: Thu, 9 Feb 2017 16:19:00 +0000 Subject: [Histonet] hand staining immunos In-Reply-To: <267846266.1416224.1486655482834@mail.yahoo.com> References: <000001d28269$b81e82f0$285b88d0$@com> <267846266.1416224.1486655482834@mail.yahoo.com> Message-ID: <0952dbba122f4678aa83759b2c98d994@MAIL01.GCU-MD.local> http://www.agilent.com/cs/library/technicaloverviews/public/08002_ihc_staining_methods.pdf -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 09, 2017 10:51 AM To: Jennifer ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] hand staining immunos You do not have to "babysit" the procedure for it has well defined/timed steps. You just need a timer and check the slides when required. As to procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren? On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet wrote: Hello, My dermatopathologist wants me to start hand staining immunos for MITF. I would really like some feedback about everyone's experiences/thoughts with manually hand staining immunos and especially with MITF. Pros and cons? I am concerned about the amount of time this will take for a tech to babysit the stain and also about the consistency of the quality in the end result. Your opinions are much appreciated. Thank you! Jennifer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From tbraud at holyredeemer.com Thu Feb 9 13:02:13 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 9 Feb 2017 19:02:13 +0000 Subject: [Histonet] Handstaining IHC for MITC Message-ID: <48E053DDF6CE074DB6A7414BA05403F81210BB@HRHEX03-HOS.holyredeemer.local> Coming from an ancient histology tech who did IHC back in the dark ages, before automation, hand staining is a piece of cake. A couple of hints: Use a "pap" pen to circle your tissue on the slide to form a barrier to keep your reagents puddled in a concentrated spot and to minimize reagent use. Use a plastic 100 slide box as an incubation chamber. Place dampened strips of paper towels in the bottom to make a moist chamber, and lay the slides flat across the filing slots. Keep the lid closed between steps. Use a reliable kit and pre-dilutes to make life easier. Best of luck. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From ranna0726 at gmail.com Thu Feb 9 13:32:49 2017 From: ranna0726 at gmail.com (Ranna Mehta) Date: Thu, 9 Feb 2017 13:32:49 -0600 Subject: [Histonet] hand staining immunos Message-ID: Hi Jennifer, How many slides are you planning to stain in routine? Once you will understand the basic, it will be a very interesting field. If you like, I can help you to walk through process with step by step. send me a message through my email.All the best and thanks. Regards Ranna On Thu, Feb 9, 2017 at 12:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Looking for a Histology Workshop (Jessica Riggleman) > 2. Re: Looking for a Histology Workshop (Jessica Riggleman) > 3. hand staining immunos (Jennifer) > 4. Re: hand staining immunos (Rene J Buesa) > 5. Re: hand staining immunos (Walter Benton) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 8 Feb 2017 18:07:24 +0000 > From: Jessica Riggleman > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Looking for a Histology Workshop > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > > Hi Everyone, > > I am looking for workshop or class for Histology/Histotechnology that > lasts anywhere from 1 week to 3 months in the U.S. > Preferably in-person and not online. > > If you have any suggestions or contacts, please let me know. > > Thank You, > Jessica > > _____________________________________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > website: http://www.globusmedical.com | email: > jriggleman at globusmedical.com > An ISO 13485 Registered Company > > Confidentiality Note: This email is confidential and intended solely for > the use of the individual to whom it is addressed. If you are not the > intended recipient, be advised that you have received this email in error > and that any use, dissemination, forwarding, printing, or copying of this > email is strictly prohibited. If you have received this email in error > please contact the sender. Any views or opinions presented are solely those > of the author and do not necessarily represent those of Globus Medical, > Inc. Although this email and any attachments are believed to be free of any > virus or other defects which might affect any computer or IT system into > which they are received, no responsibility is accepted by Globus Medical, > Inc. for any loss or damage arising in any way from the receipt or use > thereof. > > Disclaimer > > The information contained in this communication from the sender is > confidential. It is intended solely for use by the recipient and others > authorized to receive it. If you are not the recipient, you are hereby > notified that any disclosure, copying, distribution or taking action in > relation of the contents of this information is strictly prohibited and may > be unlawful. > > This email has been scanned for viruses and malware, and may have been > automatically archived by Mimecast Ltd, an innovator in Software as a > Service (SaaS) for business. Providing a safer and more useful place for > your human generated data. Specializing in; Security, archiving and > compliance. To find out more visit the Mimecast website. > > > ------------------------------ > > Message: 2 > Date: Wed, 8 Feb 2017 21:33:47 +0000 > From: Jessica Riggleman > To: Esther C Peters > Cc: "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Looking for a Histology Workshop > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Hi Esther, > > We are looking for basic training to include study design, sectioning raw > specimens for processing (i.e., how large should my specimen be?), > processing techniques (fixation, etc.), special stains, and slide reading. > This is not for HT/HTL exam preparation. > > We would prefer non-human tissues, as we typically work with large sheep > and rabbit specimens. Only two students. > > Thank You, > Jessica > > > From: Esther C Peters [mailto:epeters2 at gmu.edu] > Sent: Wednesday, February 08, 2017 4:18 PM > To: Jessica Riggleman > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: Looking for a Histology Workshop > > Is this for human or non-human tissues? How many students? > > Esther > > > _____________________________________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > > Confidentiality Note: This email is confidential and intended solely for > the use of the individual to whom it is addressed. If you are not the > intended recipient, be advised that you have received this email in error > and that any use, dissemination, forwarding, printing, or copying of this > email is strictly prohibited. If you have received this email in error > please contact the sender. Any views or opinions presented are solely those > of the author and do not necessarily represent those of Globus Medical, > Inc. Although this email and any attachments are believed to be free of any > virus or other defects which might affect any computer or IT system into > which they are received, no responsibility is accepted by Globus Medical, > Inc. for any loss or damage arising in any way from the receipt or use > thereof. > > > ________________________________________ > From: Jessica Riggleman > Sent: Wednesday, February 8, 2017 4:12:20 PM > To: Esther C Peters > Cc: histonet at lists.utsouthwestern.edu > Subject: RE: Looking for a Histology Workshop > > Hi Esther, > > We are looking for basic training to include study design, sectioning raw > specimens for processing (i.e., how large should my specimen be?), > processing techniques (fixation, etc.), special stains, and slide reading. > This is not for HT/HTL exam preparation. > > Thank You, > Jessica > > ________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > An ISO 13485 Registered Company > > website | map | email > > > > > > Confidentiality Note: This email is confidential and intended solely for > the use of the individual to whom it is addressed. If you are not the > intended recipient, be advised that you have received this email in error > and that any use, dissemination, forwarding, printing, or copying of this > email is strictly prohibited. If you have received this email in error > please contact the sender. Any views or opinions presented are solely those > of the author and do not necessarily represent those of Globus Medical, > Inc. Although this email and any attachments are believed to be free of any > virus or other defects which might affect any computer or IT system into > which they are received, no responsibility is accepted by Globus Medical, > Inc. for any loss or damage arising in any way from the receipt or use > thereof. > From: Esther C Peters [mailto:epeters2 at gmu.edu] > Sent: Wednesday, February 08, 2017 3:53 PM > To: Jessica Riggleman > Subject: Re: Looking for a Histology Workshop > > Hi Jessica, > > It might help everyone to know exactly what you want to cover in the > workshop or class. Do you need basic training, preparation for the HT/HTL > exam, slide preparation, special stains, or slide reading? What is your > goal? > > Esther > > Esther C. Peters, Ph.D. > George Mason University > Fairfax, VA 22030-4444 > ________________________________________ > From: Jessica Riggleman via Histonet > Sent: Wednesday, February 8, 2017 1:07:24 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Looking for a Histology Workshop > > > Hi Everyone, > > I am looking for workshop or class for Histology/Histotechnology that > lasts anywhere from 1 week to 3 months in the U.S. > Preferably in-person and not online. > > If you have any suggestions or contacts, please let me know. > > Thank You, > Jessica > > _____________________________________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > website: http://www.globusmedical.com | email: > jriggleman at globusmedical.com > An ISO 13485 Registered Company > > Confidentiality Note: This email is confidential and intended solely for > the use of the individual to whom it is addressed. If you are not the > intended recipient, be advised that you have received this email in error > and that any use, dissemination, forwarding, printing, or copying of this > email is strictly prohibited. If you have received this email in error > please contact the sender. Any views or opinions presented are solely those > of the author and do not necessarily represent those of Globus Medical, > Inc. Although this email and any attachments are believed to be free of any > virus or other defects which might affect any computer or IT system into > which they are received, no responsibility is accepted by Globus Medical, > Inc. for any loss or damage arising in any way from the receipt or use > thereof. > > Disclaimer > > The information contained in this communication from the sender is > confidential. It is intended solely for use by the recipient and others > authorized to receive it. If you are not the recipient, you are hereby > notified that any disclosure, copying, distribution or taking action in > relation of the contents of this information is strictly prohibited and may > be unlawful. > > This email has been scanned for viruses and malware, and may have been > automatically archived by Mimecast Ltd, an innovator in Software as a > Service (SaaS) for business. Providing a safer and more useful place for > your human generated data. Specializing in; Security, archiving and > compliance. To find out more visit the Mimecast website. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Disclaimer > > The information contained in this communication from the sender is > confidential. It is intended solely for use by the recipient and others > authorized to receive it. If you are not the recipient, you are hereby > notified that any disclosure, copying, distribution or taking action in > relation of the contents of this information is strictly prohibited and may > be unlawful. > > This email has been scanned for viruses and malware, and may have been > automatically archived by Mimecast Ltd, an innovator in Software as a > Service (SaaS) for business. Providing a safer and more useful place for > your human generated data. Specializing in; Security, archiving and > compliance. To find out more visit the Mimecast website. > > ------------------------------ > > Message: 3 > Date: Wed, 8 Feb 2017 16:16:10 -0800 > From: "Jennifer" > To: > Subject: [Histonet] hand staining immunos > Message-ID: <000001d28269$b81e82f0$285b88d0$@com> > Content-Type: text/plain; charset="us-ascii" > > Hello, > > > > My dermatopathologist wants me to start hand staining immunos for MITF. I > would really like some feedback about everyone's experiences/thoughts with > manually hand staining immunos and especially with MITF. Pros and cons? I > am > concerned about the amount of time this will take for a tech to babysit the > stain and also about the consistency of the quality in the end result. > Your > opinions are much appreciated. > > > > Thank you! > > > > Jennifer > > > > ------------------------------ > > Message: 4 > Date: Thu, 9 Feb 2017 15:51:22 +0000 (UTC) > From: Rene J Buesa > To: Jennifer , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] hand staining immunos > Message-ID: <267846266.1416224.1486655482834 at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > You do not have to "babysit" the procedure for it has well defined/timed > steps. You just need a timer and check the slides when required. As to > procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren? > > On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hello, > > > > My dermatopathologist wants me to start hand staining immunos? for MITF. I > would really like some feedback about everyone's experiences/thoughts with > manually hand staining immunos and especially with MITF. Pros and cons? I > am > concerned about the amount of time this will take for a tech to babysit the > stain and also about the consistency of the quality in the end result.? > Your > opinions are much appreciated. > > > > Thank you! > > > > Jennifer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 5 > Date: Thu, 9 Feb 2017 16:19:00 +0000 > From: Walter Benton > To: Rene J Buesa , Jennifer > > Cc: "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] hand staining immunos > Message-ID: <0952dbba122f4678aa83759b2c98d994 at MAIL01.GCU-MD.local> > Content-Type: text/plain; charset="utf-8" > > http://www.agilent.com/cs/library/technicaloverviews/ > public/08002_ihc_staining_methods.pdf > > > -----Original Message----- > From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, February 09, 2017 10:51 AM > To: Jennifer ; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] hand staining immunos > > You do not have to "babysit" the procedure for it has well defined/timed > steps. You just need a timer and check the slides when required. As to > procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren? > > On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hello, > > > > My dermatopathologist wants me to start hand staining immunos for MITF. I > would really like some feedback about everyone's experiences/thoughts with > manually hand staining immunos and especially with MITF. Pros and cons? I > am concerned about the amount of time this will take for a tech to babysit > the stain and also about the consistency of the quality in the end result. > Your opinions are much appreciated. > > > > Thank you! > > > > Jennifer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > CONFIDENTIALITY NOTICE: The information contained in this electronic > message is intended solely for the personal and confidential use of the > designated recipient(s) named above and may contain information that is > protected from disclosure under applicable law. If you are not the intended > recipient, or the employee or agent responsible for delivering it to the > intended recipient, you are hereby notified that any dissemination, > distribution or copying of this transmission is strictly prohibited. If you > have received this transmission in error, please notify the transmitting > person/department immediately by email or telephone (410) 581-5881 and > delete the message without making a copy. > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 159, Issue 8 > **************************************** > From jvickroy at SpringfieldClinic.com Thu Feb 9 14:40:23 2017 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Thu, 9 Feb 2017 20:40:23 +0000 Subject: [Histonet] Colloidal Iron and PAS with and without diastase Message-ID: <9B1A1501A800064397369BD8072E6BCA586EDCEE@E2k10DB2.springfieldclinic.com> I have probably asked this before but when performing the PAS stain with and without diastase I have been told you can charge two 88313's. When performing the colloidal iron stain the place we send it to also charges us for two stains, one with omitting the colloidal iron solution. I understand the principle in determining whether the Prussian blue staining is from the acid mucopolysaccharides or hemosiderin. How are others charging for this stain if they run two slides? And do you agree that the PAS with and without can be charged twice? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From Robyn.L.Vazquez at kp.org Thu Feb 9 18:41:44 2017 From: Robyn.L.Vazquez at kp.org (Robyn L. Vazquez) Date: Fri, 10 Feb 2017 00:41:44 +0000 Subject: [Histonet] Immunohistochemistry ghosting Message-ID: Hello, We have been experiencing problems with ghosting (inconsistent and/or no staining) mainly affecting our control area which is located at the bottom of the slide. Random antibodies markers are being affected. We were using Fisher Scientific then TruBond slides. We are using Ventana Ultras X4 and XT in which it is occurring on all machines. Ventana has come in to troubleshoot, in which we have not reached a solution. Any suggestions would be greatly appreciated Robyn L. Vazquez Kaiser Permanente Portland, Oregon NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From mgiorgi at incdx.com Thu Feb 9 19:14:27 2017 From: mgiorgi at incdx.com (Miranda Giorgi) Date: Fri, 10 Feb 2017 01:14:27 +0000 Subject: [Histonet] Immunohistochemistry ghosting In-Reply-To: References: Message-ID: Hi Robyn, We had the same issue last year and it came down to slide type for us. We were using a charged slide from Cancer Diagnostics, but have since switched to Mercedes Platinum Line Starfrost slides. We have not had any issues with "ghost tissue" since then. Miranda Giorgi, HTL (ASCP)cm Histology Manager (509)892-2744 mgiorgi at incdx.com -----Original Message----- From: Robyn L. Vazquez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 09, 2017 4:42 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Immunohistochemistry ghosting Hello, We have been experiencing problems with ghosting (inconsistent and/or no staining) mainly affecting our control area which is located at the bottom of the slide. Random antibodies markers are being affected. We were using Fisher Scientific then TruBond slides. We are using Ventana Ultras X4 and XT in which it is occurring on all machines. Ventana has come in to troubleshoot, in which we have not reached a solution. Any suggestions would be greatly appreciated Robyn L. Vazquez Kaiser Permanente Portland, Oregon NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700. From ADuddey at firsthealth.org Fri Feb 10 07:31:51 2017 From: ADuddey at firsthealth.org (Duddey, Aimee) Date: Fri, 10 Feb 2017 13:31:51 +0000 Subject: [Histonet] Immunohistochemistry ghosting In-Reply-To: References: Message-ID: <09FBA01CA9B6374A83C5C76E09E46188A3EEAE8A@EXMAIL1-FHC.firsthealth.org> When we saw this happening it was slide related, especially with it happening across all platforms. We use Cardinal Healthcare SuperFrost Plus and haven't had an issue. Every time we have tried another brand we have seen this happen to some degree. Aimee M. Duddey, MLT(ASCP) Assistant Director of Laboratory - Pathology FH Moore Regional Hospital 910-715-5286 office 910715-1944 fax -----Original Message----- From: Robyn L. Vazquez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 09, 2017 7:42 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Immunohistochemistry ghosting Hello, We have been experiencing problems with ghosting (inconsistent and/or no staining) mainly affecting our control area which is located at the bottom of the slide. Random antibodies markers are being affected. We were using Fisher Scientific then TruBond slides. We are using Ventana Ultras X4 and XT in which it is occurring on all machines. Ventana has come in to troubleshoot, in which we have not reached a solution. Any suggestions would be greatly appreciated Robyn L. Vazquez Kaiser Permanente Portland, Oregon NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbmphoto at gmail.com Fri Feb 10 12:43:56 2017 From: mbmphoto at gmail.com (Maria Mejia) Date: Fri, 10 Feb 2017 10:43:56 -0800 Subject: [Histonet] need automation system for fluorescent Multiplex IHC Message-ID: Dear All, I work in a research lab where the focus is on early stages of Alzheimer?s Disease. We are seriously considering using an automated staining system for fluorescent Multiplex IHC, for the simultaneous detection of multiple (2-6 or more) proteins of interest in FFPE brain sections. However, our lab has never used an automated staining system before, so we lack the abilities and experience to make the correct choice for our lab?s needs. Cost; the price of a USED system, since we are a research lab since a new system is more expensive, user friendly & size of system are of course major considerations as well as the consumables that go along with the system e.g. reagents, containers, & anything else to consider as well. Please, I?m reaching out to anyone (system users & vendors) who can help our lab make a smart choice. I would be most grateful for any recommendations or suggestions. I very much look forward to hearing from you. Best Maria Mejia Lead Histologist UCSF Memory & Aging Center From mashalsmom at gmail.com Fri Feb 10 13:30:27 2017 From: mashalsmom at gmail.com (Emily Horst) Date: Fri, 10 Feb 2017 14:30:27 -0500 Subject: [Histonet] New lab Message-ID: Hi everyone, I am in the process of setting up a new lab. We will process FNA specimens as cell blocks and then treat them as regular histology specimens. I am curious as to finding a proper VIP schedule for processing these specimens. Any suggestions?? Thanks in advance, Emily Horst -- Emily Horst, HT(ASCP) Office Manager Interventional Pathology of Ohio, LLC Kettering OH 45429 937-297-0028 From allanw at biomax.us Fri Feb 10 13:30:33 2017 From: allanw at biomax.us (Allan Wang) Date: Fri, 10 Feb 2017 14:30:33 -0500 Subject: [Histonet] Selling a Ventana Discovery XT Message-ID: Hello list, The last message reminded me: My lab is upgrading from the Discovery XT to the Ultra so we're selling the XT. We're offering it for a great price compared to new. It'll come with the computer, slide label printer, accessories, and all the reagents - everything needed to get started. It's only been used for 7 months and is still under the original first year service contract (doubt it will transfer though) If anyone is interested, I'd be happy to discuss the pros and cons of the system. It is located in Rockville, MD. Here is an example of a double stain: http://www.biomax.us/images/ER-Brown_HER2-Purple_core.jpg Allan Wang Lab Manager US Biomax From mbmphoto at gmail.com Fri Feb 10 13:35:00 2017 From: mbmphoto at gmail.com (Maria Mejia) Date: Fri, 10 Feb 2017 11:35:00 -0800 Subject: [Histonet] need automation system for fluorescent Multiplex IHC In-Reply-To: References: Message-ID: I forgot to mention in my email below our sections will be paraffin for Multiplex fluorescent. Thank you > On Feb 10, 2017, at 10:43 AM, Maria Mejia wrote: > > Dear All, > > I work in a research lab where the focus is on early stages of Alzheimer?s Disease. > We are seriously considering using an automated staining system for fluorescent Multiplex > IHC, for the simultaneous detection of multiple (2-6 or more) proteins of interest in > FFPE brain sections. > > However, our lab has never used an automated staining system before, so we lack > the abilities and experience to make the correct choice for our lab?s needs. > > Cost; the price of a USED system, since we are a research lab since a new system is more > expensive, user friendly & size of system are of course major considerations as well > as the consumables that go along with the system e.g. reagents, containers, & anything else > to consider as well. > > Please, I?m reaching out to anyone (system users & vendors) who can help our lab make a smart choice. > I would be most grateful for any recommendations or suggestions. I very much look forward to hearing > from you. > > Best > Maria Mejia > Lead Histologist > UCSF > Memory & Aging Center > > From sbaldwin at mhhcc.org Fri Feb 10 13:50:35 2017 From: sbaldwin at mhhcc.org (Baldwin, Kathy) Date: Fri, 10 Feb 2017 19:50:35 +0000 Subject: [Histonet] BD PROBETEC Message-ID: <0248e01a15c9439f86a0003d3cab38f4@exch02.mhhcc.org> Hi Histonetters!! We have a probetec unit that our histology department is willing to sell. Below is conformation of the manufacturer date. It was taken out of service this past fall and was in full working order. It does have a water bath and heat block (the heat block is having issues maintaining a temp). The model is BD Probetec ET, serial number: PT 2782. I will get ahold of our materials management department if this is something any of you are interested in. Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From scrochiere at nedlc.com Fri Feb 10 14:23:01 2017 From: scrochiere at nedlc.com (Steven Crochiere) Date: Fri, 10 Feb 2017 15:23:01 -0500 Subject: [Histonet] PRN Histo position Message-ID: <000d01d283db$7a7fad70$6f7f0850$@nedlc.com> Currently seeking an experienced HT for dermpath/MOHS laboratory in Springfield, MA to cover for absences and possibility to become FT in future. Contact: Terry Molnar New England Dermatology 3455 Main St Springfield, MA 01107 tmolnar at nedlc.com (413)733-9600 x319 From ian.bernard at comcast.net Sat Feb 11 08:38:14 2017 From: ian.bernard at comcast.net (ian bernard) Date: Sat, 11 Feb 2017 07:38:14 -0700 Subject: [Histonet] Fat or Breast & Lipoma Processing Schedule Sought Message-ID: <017101d28474$7a85d670$6f918350$@comcast.net> We use Safe Clear 11 (a Xylene sub) as the clearing agent on our routine processing schedule; with no intent to resort back to Xylene. Since we are taking in an influx of lipoma and breast cases, we are seeking a separate tissue processing schedule. Thus looking for an overnight schedule template that we can validate. Are there any histonetters with an overnight schedule that they are willing to share as our template to begin validation. Also, any reference source you can point too along these lines will be greatly appreciated. V/r IB From rjbuesa at yahoo.com Sat Feb 11 10:23:23 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Sat, 11 Feb 2017 16:23:23 +0000 (UTC) Subject: [Histonet] Fat or Breast & Lipoma Processing Schedule Sought In-Reply-To: <017101d28474$7a85d670$6f918350$@comcast.net> References: <017101d28474$7a85d670$6f918350$@comcast.net> Message-ID: <206226653.2780192.1486830203036@mail.yahoo.com> You can find all my xylene-free processing schedules at the HistoNet archives. They use 2-propanol ? mineral oil ? paraffin.Ren?? On Saturday, February 11, 2017 9:44 AM, ian bernard via Histonet wrote: We use Safe Clear 11 (a Xylene sub) as the clearing agent on our routine processing schedule; with no intent to resort back to Xylene.? Since we are taking in an influx of lipoma and breast cases, we are seeking a separate tissue processing schedule.? Thus looking for an overnight schedule template that we can validate. Are there any histonetters with an overnight schedule that they are willing to share as our template to begin validation.? Also, any reference source you can point too along these lines will be greatly appreciated. V/r IB _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michael_michael13 at yahoo.com Sun Feb 12 12:19:40 2017 From: michael_michael13 at yahoo.com (Michael Michael) Date: Sun, 12 Feb 2017 18:19:40 +0000 (UTC) Subject: [Histonet] Histonet Digest, Vol 159, Issue 10 References: <19364295.2925985.1486923580048.ref@mail.yahoo.com> Message-ID: <19364295.2925985.1486923580048@mail.yahoo.com> blockquote, div.yahoo_quoted { margin-left: 0 !important; border-left:1px #715FFA solid !important; padding-left:1ex !important; background-color:white !important; } On Saturday, February 11, 2017 1:00 PM, "histonet-request at lists.utsouthwestern.edu" wrote: ----- Forwarded Message ----- Send Histonet mailing list submissions to ??? histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. need automation system for fluorescent Multiplex IHC (Maria Mejia) ? 2. New lab (Emily Horst) ? 3. Selling a Ventana Discovery XT (Allan Wang) ? 4. Re: need automation system for fluorescent Multiplex IHC ? ? ? (Maria Mejia) ? 5. BD PROBETEC (Baldwin, Kathy) ? 6. PRN Histo position (Steven Crochiere) ? 7. Fat or Breast & Lipoma Processing Schedule Sought (ian bernard) ? 8. Re: Fat or Breast & Lipoma Processing Schedule Sought ? ? ? (Rene J Buesa) Dear All, I work in a research lab where the focus is on early stages of Alzheimer?s Disease. We are seriously considering using an automated staining system for fluorescent Multiplex IHC, for the simultaneous detection of multiple (2-6 or more) proteins of interest in FFPE brain sections. However, our lab has never used an automated staining system before, so we lack the abilities and experience to make the correct choice for our lab?s needs. Cost; the price of a USED system, since we are a research lab since a new system is more expensive, user friendly & size of system are of course major considerations as well as the consumables that go along with the system e.g. reagents, containers, & anything else to consider as well. Please, I?m reaching out to anyone (system users & vendors) who can help our lab make a smart choice. I would be most grateful for any recommendations or suggestions. I very much look forward to hearing from you. Best Maria Mejia Lead Histologist UCSF Memory & Aging Center Hi everyone, I am in the process of setting up a new lab. We will process FNA specimens as cell blocks and then treat them as regular histology specimens. I am curious as to finding a proper VIP schedule for processing these specimens. Any suggestions?? Thanks in advance, Emily Horst -- Emily Horst, HT(ASCP) Office Manager Interventional Pathology of Ohio, LLC Kettering OH 45429 937-297-0028 Hello list, The last message reminded me: My lab is upgrading from the Discovery XT to the Ultra so we're selling the XT. We're offering it for a great price compared to new. It'll come with the computer, slide label printer, accessories, and all the reagents - everything needed to get started. It's only been used for 7 months and is still under the original first year service contract (doubt it will transfer though) If anyone is interested, I'd be happy to discuss the pros and cons of the system. It is located in Rockville, MD. Here is an example of a double stain: http://www.biomax.us/images/ER-Brown_HER2-Purple_core.jpg Allan Wang Lab Manager US Biomax I forgot to mention in my email below our sections will be paraffin for Multiplex fluorescent. Thank you > On Feb 10, 2017, at 10:43 AM, Maria Mejia wrote: > > Dear All, > > I work in a research lab where the focus is on early stages of Alzheimer?s Disease. > We are seriously considering using an automated staining system for fluorescent Multiplex > IHC, for the simultaneous detection of multiple (2-6 or more) proteins of interest in > FFPE brain sections. > > However, our lab has never used an automated staining system before, so we lack > the abilities and experience to make the correct choice for our lab?s needs. > > Cost; the price of a USED system, since we are a research lab since a new system is more > expensive, user friendly & size of system are of course major considerations as well > as the consumables that go along with the system e.g. reagents, containers, & anything else > to consider as well. > > Please, I?m reaching out to anyone (system users & vendors) who can help our lab make a smart choice. > I would be most grateful for any recommendations or suggestions. I very much look forward to hearing > from you. > > Best > Maria Mejia > Lead Histologist > UCSF > Memory & Aging Center > > Hi Histonetters!! ? ? ? ? ? ? ? We have a probetec unit that our histology department is willing to sell.? Below is conformation of the manufacturer date.? It was taken out of service this past fall and was in full working order.? It does have a water bath and heat block (the heat block is having issues maintaining a temp).? The model is BD Probetec ET, serial number: PT 2782.? I will get ahold of our materials management department if this is something any of you are interested in. Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana? 47546 Office 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Currently seeking an experienced HT for dermpath/MOHS laboratory in Springfield, MA to cover for absences and possibility to become FT? in future. Contact: Terry Molnar New England Dermatology 3455 Main St Springfield, MA 01107 tmolnar at nedlc.com (413)733-9600 x319 We use Safe Clear 11 (a Xylene sub) as the clearing agent on our routine processing schedule; with no intent to resort back to Xylene.? Since we are taking in an influx of lipoma and breast cases, we are seeking a separate tissue processing schedule.? Thus looking for an overnight schedule template that we can validate. Are there any histonetters with an overnight schedule that they are willing to share as our template to begin validation.? Also, any reference source you can point too along these lines will be greatly appreciated. V/r IB You can find all my xylene-free processing schedules at the HistoNet archives. They use 2-propanol ? mineral oil ? paraffin.Ren?? ? ? On Saturday, February 11, 2017 9:44 AM, ian bernard via Histonet wrote: We use Safe Clear 11 (a Xylene sub) as the clearing agent on our routine processing schedule; with no intent to resort back to Xylene.? Since we are taking in an influx of lipoma and breast cases, we are seeking a separate tissue processing schedule.? Thus looking for an overnight schedule template that we can validate. Are there any histonetters with an overnight schedule that they are willing to share as our template to begin validation.? Also, any reference source you can point too along these lines will be greatly appreciated. V/r IB _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From srishan at mail.holyname.org Mon Feb 13 07:19:08 2017 From: srishan at mail.holyname.org (srishan at mail.holyname.org) Date: Mon, 13 Feb 2017 08:19:08 -0500 Subject: [Histonet] Basel cell cocktail Message-ID: Hi Histonetters, If someone out there are using Basal cell cocktail from Ventana(34 Beta E12+ P63), could you let me know how the charges are posted for this cocktail. Thanks Nirmala Nirmala Srishan Histology Supervisor Holy Name Medical Center 718 Teaneck Road Teaneck NJ 07666 Lab: 201 833 3023 Office: 201 541 6328 Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From mills at 3scan.com Mon Feb 13 09:06:46 2017 From: mills at 3scan.com (Caroline Miller) Date: Mon, 13 Feb 2017 07:06:46 -0800 Subject: [Histonet] hand staining immunos In-Reply-To: References: Message-ID: This is a great system for semi-automated immuno. I find the results a little cleaner than performing the technique straight on the slide. No need to buy the whole set up (it is just a glorified timer)- just the racks and clips are enough! https://www.fishersci.com/shop/products/thermo-scientific-shandon-sequenza-immunostaining-center-2/p-4010433 mills On Thu, Feb 9, 2017 at 11:32 AM, Ranna Mehta via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Jennifer, > > How many slides are you planning to stain in routine? Once you will > understand the basic, it will be a very interesting field. If you like, I > can help you to walk through process with step by step. send me a message > through my email.All the best and thanks. > > Regards > Ranna > > On Thu, Feb 9, 2017 at 12:00 PM, utsouthwestern.edu> > wrote: > > > Send Histonet mailing list submissions to > > histonet at lists.utsouthwestern.edu > > > > To subscribe or unsubscribe via the World Wide Web, visit > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > or, via email, send a message with subject or body 'help' to > > histonet-request at lists.utsouthwestern.edu > > > > You can reach the person managing the list at > > histonet-owner at lists.utsouthwestern.edu > > > > When replying, please edit your Subject line so it is more specific > > than "Re: Contents of Histonet digest..." > > > > > > Today's Topics: > > > > 1. Looking for a Histology Workshop (Jessica Riggleman) > > 2. Re: Looking for a Histology Workshop (Jessica Riggleman) > > 3. hand staining immunos (Jennifer) > > 4. Re: hand staining immunos (Rene J Buesa) > > 5. Re: hand staining immunos (Walter Benton) > > > > > > ---------------------------------------------------------------------- > > > > Message: 1 > > Date: Wed, 8 Feb 2017 18:07:24 +0000 > > From: Jessica Riggleman > > To: "histonet at lists.utsouthwestern.edu" > > > > Subject: [Histonet] Looking for a Histology Workshop > > Message-ID: > > > > Content-Type: text/plain; charset=UTF-8 > > > > > > Hi Everyone, > > > > I am looking for workshop or class for Histology/Histotechnology that > > lasts anywhere from 1 week to 3 months in the U.S. > > Preferably in-person and not online. > > > > If you have any suggestions or contacts, please let me know. > > > > Thank You, > > Jessica > > > > _____________________________________________________________________ > > > > Jessica Riggleman | Research Associate > > > > Globus Medical, Inc. > > Valley Forge Business Center > > 2560 General Armistead Avenue | Audubon, PA 19403 > > Ph: (610) 930-1800 ext. 2583 | Fax: > > website: http://www.globusmedical.com | email: > > jriggleman at globusmedical.com > > An ISO 13485 Registered Company > > > > Confidentiality Note: This email is confidential and intended solely for > > the use of the individual to whom it is addressed. If you are not the > > intended recipient, be advised that you have received this email in error > > and that any use, dissemination, forwarding, printing, or copying of this > > email is strictly prohibited. If you have received this email in error > > please contact the sender. Any views or opinions presented are solely > those > > of the author and do not necessarily represent those of Globus Medical, > > Inc. Although this email and any attachments are believed to be free of > any > > virus or other defects which might affect any computer or IT system into > > which they are received, no responsibility is accepted by Globus Medical, > > Inc. for any loss or damage arising in any way from the receipt or use > > thereof. > > > > Disclaimer > > > > The information contained in this communication from the sender is > > confidential. It is intended solely for use by the recipient and others > > authorized to receive it. If you are not the recipient, you are hereby > > notified that any disclosure, copying, distribution or taking action in > > relation of the contents of this information is strictly prohibited and > may > > be unlawful. > > > > This email has been scanned for viruses and malware, and may have been > > automatically archived by Mimecast Ltd, an innovator in Software as a > > Service (SaaS) for business. Providing a safer and more useful place for > > your human generated data. Specializing in; Security, archiving and > > compliance. To find out more visit the Mimecast website. > > > > > > ------------------------------ > > > > Message: 2 > > Date: Wed, 8 Feb 2017 21:33:47 +0000 > > From: Jessica Riggleman > > To: Esther C Peters > > Cc: "histonet at lists.utsouthwestern.edu" > > > > Subject: Re: [Histonet] Looking for a Histology Workshop > > Message-ID: > > > > Content-Type: text/plain; charset=UTF-8 > > > > Hi Esther, > > > > We are looking for basic training to include study design, sectioning raw > > specimens for processing (i.e., how large should my specimen be?), > > processing techniques (fixation, etc.), special stains, and slide > reading. > > This is not for HT/HTL exam preparation. > > > > We would prefer non-human tissues, as we typically work with large sheep > > and rabbit specimens. Only two students. > > > > Thank You, > > Jessica > > > > > > From: Esther C Peters [mailto:epeters2 at gmu.edu] > > Sent: Wednesday, February 08, 2017 4:18 PM > > To: Jessica Riggleman > > Cc: histonet at lists.utsouthwestern.edu > > Subject: Re: Looking for a Histology Workshop > > > > Is this for human or non-human tissues? How many students? > > > > Esther > > > > > > _____________________________________________________________________ > > > > Jessica Riggleman | Research Associate > > > > Globus Medical, Inc. > > Valley Forge Business Center > > 2560 General Armistead Avenue | Audubon, PA 19403 > > Ph: (610) 930-1800 ext. 2583 | Fax: > > > > Confidentiality Note: This email is confidential and intended solely for > > the use of the individual to whom it is addressed. If you are not the > > intended recipient, be advised that you have received this email in error > > and that any use, dissemination, forwarding, printing, or copying of this > > email is strictly prohibited. If you have received this email in error > > please contact the sender. Any views or opinions presented are solely > those > > of the author and do not necessarily represent those of Globus Medical, > > Inc. Although this email and any attachments are believed to be free of > any > > virus or other defects which might affect any computer or IT system into > > which they are received, no responsibility is accepted by Globus Medical, > > Inc. for any loss or damage arising in any way from the receipt or use > > thereof. > > > > > > ________________________________________ > > From: Jessica Riggleman > > Sent: Wednesday, February 8, 2017 4:12:20 PM > > To: Esther C Peters > > Cc: histonet at lists.utsouthwestern.edu > > Subject: RE: Looking for a Histology Workshop > > > > Hi Esther, > > > > We are looking for basic training to include study design, sectioning raw > > specimens for processing (i.e., how large should my specimen be?), > > processing techniques (fixation, etc.), special stains, and slide > reading. > > This is not for HT/HTL exam preparation. > > > > Thank You, > > Jessica > > > > ________________________________________ > > > > Jessica Riggleman | Research Associate > > > > Globus Medical, Inc. > > Valley Forge Business Center > > 2560 General Armistead Avenue | Audubon, PA 19403 > > Ph: (610) 930-1800 ext. 2583 | Fax: > > An ISO 13485 Registered Company > > > > website | map | email > > > > > > > > > > > > Confidentiality Note: This email is confidential and intended solely for > > the use of the individual to whom it is addressed. If you are not the > > intended recipient, be advised that you have received this email in error > > and that any use, dissemination, forwarding, printing, or copying of this > > email is strictly prohibited. If you have received this email in error > > please contact the sender. Any views or opinions presented are solely > those > > of the author and do not necessarily represent those of Globus Medical, > > Inc. Although this email and any attachments are believed to be free of > any > > virus or other defects which might affect any computer or IT system into > > which they are received, no responsibility is accepted by Globus Medical, > > Inc. for any loss or damage arising in any way from the receipt or use > > thereof. > > From: Esther C Peters [mailto:epeters2 at gmu.edu] > > Sent: Wednesday, February 08, 2017 3:53 PM > > To: Jessica Riggleman > > Subject: Re: Looking for a Histology Workshop > > > > Hi Jessica, > > > > It might help everyone to know exactly what you want to cover in the > > workshop or class. Do you need basic training, preparation for the HT/HTL > > exam, slide preparation, special stains, or slide reading? What is your > > goal? > > > > Esther > > > > Esther C. Peters, Ph.D. > > George Mason University > > Fairfax, VA 22030-4444 > > ________________________________________ > > From: Jessica Riggleman via Histonet > > Sent: Wednesday, February 8, 2017 1:07:24 PM > > To: histonet at lists.utsouthwestern.edu > > Subject: [Histonet] Looking for a Histology Workshop > > > > > > Hi Everyone, > > > > I am looking for workshop or class for Histology/Histotechnology that > > lasts anywhere from 1 week to 3 months in the U.S. > > Preferably in-person and not online. > > > > If you have any suggestions or contacts, please let me know. > > > > Thank You, > > Jessica > > > > _____________________________________________________________________ > > > > Jessica Riggleman | Research Associate > > > > Globus Medical, Inc. > > Valley Forge Business Center > > 2560 General Armistead Avenue | Audubon, PA 19403 > > Ph: (610) 930-1800 ext. 2583 | Fax: > > website: http://www.globusmedical.com | email: > > jriggleman at globusmedical.com > > An ISO 13485 Registered Company > > > > Confidentiality Note: This email is confidential and intended solely for > > the use of the individual to whom it is addressed. If you are not the > > intended recipient, be advised that you have received this email in error > > and that any use, dissemination, forwarding, printing, or copying of this > > email is strictly prohibited. If you have received this email in error > > please contact the sender. Any views or opinions presented are solely > those > > of the author and do not necessarily represent those of Globus Medical, > > Inc. Although this email and any attachments are believed to be free of > any > > virus or other defects which might affect any computer or IT system into > > which they are received, no responsibility is accepted by Globus Medical, > > Inc. for any loss or damage arising in any way from the receipt or use > > thereof. > > > > Disclaimer > > > > The information contained in this communication from the sender is > > confidential. It is intended solely for use by the recipient and others > > authorized to receive it. If you are not the recipient, you are hereby > > notified that any disclosure, copying, distribution or taking action in > > relation of the contents of this information is strictly prohibited and > may > > be unlawful. > > > > This email has been scanned for viruses and malware, and may have been > > automatically archived by Mimecast Ltd, an innovator in Software as a > > Service (SaaS) for business. Providing a safer and more useful place for > > your human generated data. Specializing in; Security, archiving and > > compliance. To find out more visit the Mimecast website. > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Disclaimer > > > > The information contained in this communication from the sender is > > confidential. It is intended solely for use by the recipient and others > > authorized to receive it. If you are not the recipient, you are hereby > > notified that any disclosure, copying, distribution or taking action in > > relation of the contents of this information is strictly prohibited and > may > > be unlawful. > > > > This email has been scanned for viruses and malware, and may have been > > automatically archived by Mimecast Ltd, an innovator in Software as a > > Service (SaaS) for business. Providing a safer and more useful place for > > your human generated data. Specializing in; Security, archiving and > > compliance. To find out more visit the Mimecast website. > > > > ------------------------------ > > > > Message: 3 > > Date: Wed, 8 Feb 2017 16:16:10 -0800 > > From: "Jennifer" > > To: > > Subject: [Histonet] hand staining immunos > > Message-ID: <000001d28269$b81e82f0$285b88d0$@com> > > Content-Type: text/plain; charset="us-ascii" > > > > Hello, > > > > > > > > My dermatopathologist wants me to start hand staining immunos for MITF. > I > > would really like some feedback about everyone's experiences/thoughts > with > > manually hand staining immunos and especially with MITF. Pros and cons? I > > am > > concerned about the amount of time this will take for a tech to babysit > the > > stain and also about the consistency of the quality in the end result. > > Your > > opinions are much appreciated. > > > > > > > > Thank you! > > > > > > > > Jennifer > > > > > > > > ------------------------------ > > > > Message: 4 > > Date: Thu, 9 Feb 2017 15:51:22 +0000 (UTC) > > From: Rene J Buesa > > To: Jennifer , > > "histonet at lists.utsouthwestern.edu" > > > > Subject: Re: [Histonet] hand staining immunos > > Message-ID: <267846266.1416224.1486655482834 at mail.yahoo.com> > > Content-Type: text/plain; charset=UTF-8 > > > > You do not have to "babysit" the procedure for it has well defined/timed > > steps. You just need a timer and check the slides when required. As to > > procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren? > > > > On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet < > > histonet at lists.utsouthwestern.edu> wrote: > > > > > > Hello, > > > > > > > > My dermatopathologist wants me to start hand staining immunos? for MITF. > I > > would really like some feedback about everyone's experiences/thoughts > with > > manually hand staining immunos and especially with MITF. Pros and cons? I > > am > > concerned about the amount of time this will take for a tech to babysit > the > > stain and also about the consistency of the quality in the end result.? > > Your > > opinions are much appreciated. > > > > > > > > Thank you! > > > > > > > > Jennifer > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > ------------------------------ > > > > Message: 5 > > Date: Thu, 9 Feb 2017 16:19:00 +0000 > > From: Walter Benton > > To: Rene J Buesa , Jennifer > > > > Cc: "histonet at lists.utsouthwestern.edu" > > > > Subject: Re: [Histonet] hand staining immunos > > Message-ID: <0952dbba122f4678aa83759b2c98d994 at MAIL01.GCU-MD.local> > > Content-Type: text/plain; charset="utf-8" > > > > http://www.agilent.com/cs/library/technicaloverviews/ > > public/08002_ihc_staining_methods.pdf > > > > > > -----Original Message----- > > From: Rene J Buesa via Histonet [mailto:histonet at lists. > utsouthwestern.edu] > > Sent: Thursday, February 09, 2017 10:51 AM > > To: Jennifer ; histonet at lists.utsouthwestern.edu > > Subject: Re: [Histonet] hand staining immunos > > > > You do not have to "babysit" the procedure for it has well defined/timed > > steps. You just need a timer and check the slides when required. As to > > procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren? > > > > On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet < > > histonet at lists.utsouthwestern.edu> wrote: > > > > > > Hello, > > > > > > > > My dermatopathologist wants me to start hand staining immunos for MITF. > I > > would really like some feedback about everyone's experiences/thoughts > with > > manually hand staining immunos and especially with MITF. Pros and cons? I > > am concerned about the amount of time this will take for a tech to > babysit > > the stain and also about the consistency of the quality in the end > result. > > Your opinions are much appreciated. > > > > > > > > Thank you! > > > > > > > > Jennifer > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: The information contained in this electronic > > message is intended solely for the personal and confidential use of the > > designated recipient(s) named above and may contain information that is > > protected from disclosure under applicable law. If you are not the > intended > > recipient, or the employee or agent responsible for delivering it to the > > intended recipient, you are hereby notified that any dissemination, > > distribution or copying of this transmission is strictly prohibited. If > you > > have received this transmission in error, please notify the transmitting > > person/department immediately by email or telephone (410) 581-5881 and > > delete the message without making a copy. > > > > ------------------------------ > > > > Subject: Digest Footer > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > > > End of Histonet Digest, Vol 159, Issue 8 > > **************************************** > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From relia1 at earthlink.net Mon Feb 13 09:19:55 2017 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 13 Feb 2017 10:19:55 -0500 Subject: [Histonet] RELIA HOT JOB ALERT!! 2-13-2017 Exciting opportunities Nationwide!!! Message-ID: <017601d2860c$a1ac0320$e5040960$@earthlink.net> Hello Histonetters, I hope you had a great weekend and are looking forward to a fantastic week! Since its only Monday and my phone is ringing off the hook with new opportunities I wanted to do a quick post to tell you about the positions that I am working on and am MOST excited about. Why am I excited about these positions?? Because these clients offer excellent compensation, benefits, relocation assistance and leading edge training! But most importantly they are ready to interview and hire ASAP. All of these positions are full time and permanent. These are the histology positions that I am most excited about! LEADERSHIP/SUPERVISORY: Histology Lab Manager ? San Diego, CA ? A RELIA EXCLUSIVE!! Histology Supervisor ? Irvine, CA Lead Histotechnologist ? Run your own lab ? Cheyenne, WY TECHNICIANS/TECHNOLOGISTS: Austin, TX Tyler, TX Longview, TX Modesto, CA San Diego, CA Elizabeth, NJ St. Louis, MO Portland, ME ? IHC Specialist Ft. Myers, FL ? Mohs/Dermpath Kingsport, TN Syracuse, NY If you happen to know anyone, I am also recruiting for: ? Pathologists Assistant in San Diego, CA ? Cytotech with some histo experience in Soldotna, AK ? Cytology Supervisor in the Chicago Area. If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I place someone you refer, You will earn a referral fee. I can be reached toll free at the office at 866-607-3542 Or Email me at relia1 at earthlink.net Or You can always catch me on cell call or text me at 407-353-5070. ? Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From paula at excaliburpathology.com Mon Feb 13 09:37:29 2017 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Mon, 13 Feb 2017 15:37:29 +0000 (UTC) Subject: [Histonet] hand staining immunos In-Reply-To: References: Message-ID: <665297508.3741906.1487000249355@mail.yahoo.com> I agree that the Sequenza would be the way to go for your lab.? It is what I use and the cover-plates can be washed and reused.?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Caroline Miller via Histonet To: Ranna Mehta Cc: "Histonet at Lists. Edu" Sent: Monday, February 13, 2017 9:06 AM Subject: Re: [Histonet] hand staining immunos This is a great system for semi-automated immuno. I find the results a little cleaner than performing the technique straight on the slide. No need to buy the whole set up (it is just a glorified timer)- just the racks and clips are enough! https://www.fishersci.com/shop/products/thermo-scientific-shandon-sequenza-immunostaining-center-2/p-4010433 mills On Thu, Feb 9, 2017 at 11:32 AM, Ranna Mehta via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Jennifer, > >? How many slides are you planning to stain in routine?? Once you will > understand the basic, it will be a very interesting field. If you like, I > can help you to walk through process with step by step. send me a message > through my email.All the best and thanks. > > Regards > Ranna > > On Thu, Feb 9, 2017 at 12:00 PM, utsouthwestern.edu> > wrote: > > > Send Histonet mailing list submissions to > >? ? ? ? histonet at lists.utsouthwestern.edu > > > > To subscribe or unsubscribe via the World Wide Web, visit > >? ? ? ? http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > or, via email, send a message with subject or body 'help' to > >? ? ? ? histonet-request at lists.utsouthwestern.edu > > > > You can reach the person managing the list at > >? ? ? ? histonet-owner at lists.utsouthwestern.edu > > > > When replying, please edit your Subject line so it is more specific > > than "Re: Contents of Histonet digest..." > > > > > > Today's Topics: > > > >? ? 1. Looking for a Histology Workshop (Jessica Riggleman) > >? ? 2. Re: Looking for a Histology Workshop (Jessica Riggleman) > >? ? 3. hand staining immunos (Jennifer) > >? ? 4. Re: hand staining immunos (Rene J Buesa) > >? ? 5. Re: hand staining immunos (Walter Benton) > > > > > > ---------------------------------------------------------------------- > > > > Message: 1 > > Date: Wed, 8 Feb 2017 18:07:24 +0000 > > From: Jessica Riggleman > > To: "histonet at lists.utsouthwestern.edu" > >? ? ? ? > > Subject: [Histonet] Looking for a Histology Workshop > > Message-ID: > >? ? ? ? > > Content-Type: text/plain; charset=UTF-8 > > > > > > Hi Everyone, > > > > I am looking for workshop or class for Histology/Histotechnology that > > lasts anywhere from 1 week to 3 months in the U.S. > > Preferably in-person and not online. > > > > If you have any suggestions or contacts, please let me know. > > > > Thank You, > > Jessica > > > > _____________________________________________________________________ > > > > Jessica Riggleman | Research Associate > > > > Globus Medical, Inc. > > Valley Forge Business Center > > 2560 General Armistead Avenue | Audubon, PA 19403 > > Ph: (610) 930-1800 ext. 2583 | Fax: > > website: http://www.globusmedical.com | email: > > jriggleman at globusmedical.com > > An ISO 13485 Registered Company > > > > Confidentiality Note:? This email is confidential and intended solely for > > the use of the individual to whom it is addressed. If you are not the > > intended recipient, be advised that you have received this email in error > > and that any use, dissemination, forwarding, printing, or copying of this > > email is strictly prohibited. If you have received this email in error > > please contact the sender. Any views or opinions presented are solely > those > > of the author and do not necessarily represent those of Globus Medical, > > Inc. Although this email and any attachments are believed to be free of > any > > virus or other defects which might affect any computer or IT system into > > which they are received, no responsibility is accepted by Globus Medical, > > Inc. for any loss or damage arising in any way from the receipt or use > > thereof. > > > > Disclaimer > > > > The information contained in this communication from the sender is > > confidential. It is intended solely for use by the recipient and others > > authorized to receive it. If you are not the recipient, you are hereby > > notified that any disclosure, copying, distribution or taking action in > > relation of the contents of this information is strictly prohibited and > may > > be unlawful. > > > > This email has been scanned for viruses and malware, and may have been > > automatically archived by Mimecast Ltd, an innovator in Software as a > > Service (SaaS) for business. Providing a safer and more useful place for > > your human generated data. Specializing in; Security, archiving and > > compliance. To find out more visit the Mimecast website. > > > > > > ------------------------------ > > > > Message: 2 > > Date: Wed, 8 Feb 2017 21:33:47 +0000 > > From: Jessica Riggleman > > To: Esther C Peters > > Cc: "histonet at lists.utsouthwestern.edu" > >? ? ? ? > > Subject: Re: [Histonet] Looking for a Histology Workshop > > Message-ID: > >? ? ? ? > > Content-Type: text/plain; charset=UTF-8 > > > > Hi Esther, > > > > We are looking for basic training to include study design, sectioning raw > > specimens for processing (i.e., how large should my specimen be?), > > processing techniques (fixation, etc.), special stains, and slide > reading. > > This is not for HT/HTL exam preparation. > > > > We would prefer non-human tissues, as we typically work with large sheep > > and rabbit specimens. Only two students. > > > > Thank You, > > Jessica > > > > > > From: Esther C Peters [mailto:epeters2 at gmu.edu] > > Sent: Wednesday, February 08, 2017 4:18 PM > > To: Jessica Riggleman > > Cc: histonet at lists.utsouthwestern.edu > > Subject: Re: Looking for a Histology Workshop > > > > Is this for human or non-human tissues? How many students? > > > > Esther > > > > > > _____________________________________________________________________ > > > > Jessica Riggleman? | Research Associate > > > > Globus Medical, Inc. > > Valley Forge Business Center > > 2560 General Armistead Avenue | Audubon, PA 19403 > > Ph: (610) 930-1800 ext. 2583 | Fax: > > > > Confidentiality Note:? This email is confidential and intended solely for > > the use of the individual to whom it is addressed. If you are not the > > intended recipient, be advised that you have received this email in error > > and that any use, dissemination, forwarding, printing, or copying of this > > email is strictly prohibited. If you have received this email in error > > please contact the sender. Any views or opinions presented are solely > those > > of the author and do not necessarily represent those of Globus Medical, > > Inc. Although this email and any attachments are believed to be free of > any > > virus or other defects which might affect any computer or IT system into > > which they are received, no responsibility is accepted by Globus Medical, > > Inc. for any loss or damage arising in any way from the receipt or use > > thereof. > > > > > > ________________________________________ > > From: Jessica Riggleman > > Sent: Wednesday, February 8, 2017 4:12:20 PM > > To: Esther C Peters > > Cc: histonet at lists.utsouthwestern.edu > > Subject: RE: Looking for a Histology Workshop > > > > Hi Esther, > > > > We are looking for basic training to include study design, sectioning raw > > specimens for processing (i.e., how large should my specimen be?), > > processing techniques (fixation, etc.), special stains, and slide > reading. > > This is not for HT/HTL exam preparation. > > > > Thank You, > > Jessica > > > > ________________________________________ > > > > Jessica Riggleman? | Research Associate > > > > Globus Medical, Inc. > > Valley Forge Business Center > > 2560 General Armistead Avenue | Audubon, PA 19403 > > Ph: (610) 930-1800 ext. 2583 | Fax: > > An ISO 13485 Registered Company > > > > website | map | email > > > > > > > > > > > > Confidentiality Note:? This email is confidential and intended solely for > > the use of the individual to whom it is addressed. If you are not the > > intended recipient, be advised that you have received this email in error > > and that any use, dissemination, forwarding, printing, or copying of this > > email is strictly prohibited. If you have received this email in error > > please contact the sender. Any views or opinions presented are solely > those > > of the author and do not necessarily represent those of Globus Medical, > > Inc. Although this email and any attachments are believed to be free of > any > > virus or other defects which might affect any computer or IT system into > > which they are received, no responsibility is accepted by Globus Medical, > > Inc. for any loss or damage arising in any way from the receipt or use > > thereof. > > From: Esther C Peters [mailto:epeters2 at gmu.edu] > > Sent: Wednesday, February 08, 2017 3:53 PM > > To: Jessica Riggleman > > Subject: Re: Looking for a Histology Workshop > > > > Hi Jessica, > > > > It might help everyone to know exactly what you want to cover in the > > workshop or class. Do you need basic training, preparation for the HT/HTL > > exam, slide preparation, special stains, or slide reading? What is your > > goal? > > > > Esther > > > > Esther C. Peters, Ph.D. > > George Mason University > > Fairfax, VA 22030-4444 > > ________________________________________ > > From: Jessica Riggleman via Histonet > > Sent: Wednesday, February 8, 2017 1:07:24 PM > > To: histonet at lists.utsouthwestern.edu > > Subject: [Histonet] Looking for a Histology Workshop > > > > > > Hi Everyone, > > > > I am looking for workshop or class for Histology/Histotechnology that > > lasts anywhere from 1 week to 3 months in the U.S. > > Preferably in-person and not online. > > > > If you have any suggestions or contacts, please let me know. > > > > Thank You, > > Jessica > > > > _____________________________________________________________________ > > > > Jessica Riggleman | Research Associate > > > > Globus Medical, Inc. > > Valley Forge Business Center > > 2560 General Armistead Avenue | Audubon, PA 19403 > > Ph: (610) 930-1800 ext. 2583 | Fax: > > website: http://www.globusmedical.com | email: > > jriggleman at globusmedical.com > > An ISO 13485 Registered Company > > > > Confidentiality Note:? This email is confidential and intended solely for > > the use of the individual to whom it is addressed. If you are not the > > intended recipient, be advised that you have received this email in error > > and that any use, dissemination, forwarding, printing, or copying of this > > email is strictly prohibited. If you have received this email in error > > please contact the sender. Any views or opinions presented are solely > those > > of the author and do not necessarily represent those of Globus Medical, > > Inc. Although this email and any attachments are believed to be free of > any > > virus or other defects which might affect any computer or IT system into > > which they are received, no responsibility is accepted by Globus Medical, > > Inc. for any loss or damage arising in any way from the receipt or use > > thereof. > > > > Disclaimer > > > > The information contained in this communication from the sender is > > confidential. It is intended solely for use by the recipient and others > > authorized to receive it. If you are not the recipient, you are hereby > > notified that any disclosure, copying, distribution or taking action in > > relation of the contents of this information is strictly prohibited and > may > > be unlawful. > > > > This email has been scanned for viruses and malware, and may have been > > automatically archived by Mimecast Ltd, an innovator in Software as a > > Service (SaaS) for business. Providing a safer and more useful place for > > your human generated data. Specializing in; Security, archiving and > > compliance. To find out more visit the Mimecast website. > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Disclaimer > > > > The information contained in this communication from the sender is > > confidential. It is intended solely for use by the recipient and others > > authorized to receive it. If you are not the recipient, you are hereby > > notified that any disclosure, copying, distribution or taking action in > > relation of the contents of this information is strictly prohibited and > may > > be unlawful. > > > > This email has been scanned for viruses and malware, and may have been > > automatically archived by Mimecast Ltd, an innovator in Software as a > > Service (SaaS) for business. Providing a safer and more useful place for > > your human generated data. Specializing in; Security, archiving and > > compliance. To find out more visit the Mimecast website. > > > > ------------------------------ > > > > Message: 3 > > Date: Wed, 8 Feb 2017 16:16:10 -0800 > > From: "Jennifer" > > To: > > Subject: [Histonet] hand staining immunos > > Message-ID: <000001d28269$b81e82f0$285b88d0$@com> > > Content-Type: text/plain;? ? ? charset="us-ascii" > > > > Hello, > > > > > > > > My dermatopathologist wants me to start hand staining immunos? for MITF. > I > > would really like some feedback about everyone's experiences/thoughts > with > > manually hand staining immunos and especially with MITF. Pros and cons? I > > am > > concerned about the amount of time this will take for a tech to babysit > the > > stain and also about the consistency of the quality in the end result. > > Your > > opinions are much appreciated. > > > > > > > > Thank you! > > > > > > > > Jennifer > > > > > > > > ------------------------------ > > > > Message: 4 > > Date: Thu, 9 Feb 2017 15:51:22 +0000 (UTC) > > From: Rene J Buesa > > To: Jennifer , > >? ? ? ? "histonet at lists.utsouthwestern.edu" > >? ? ? ? > > Subject: Re: [Histonet] hand staining immunos > > Message-ID: <267846266.1416224.1486655482834 at mail.yahoo.com> > > Content-Type: text/plain; charset=UTF-8 > > > > You do not have to "babysit" the procedure for it has well defined/timed > > steps. You just need a timer and check the slides when required. As to > > procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren? > > > >? ? On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet < > > histonet at lists.utsouthwestern.edu> wrote: > > > > > >? Hello, > > > > > > > > My dermatopathologist wants me to start hand staining immunos? for MITF. > I > > would really like some feedback about everyone's experiences/thoughts > with > > manually hand staining immunos and especially with MITF. Pros and cons? I > > am > > concerned about the amount of time this will take for a tech to babysit > the > > stain and also about the consistency of the quality in the end result.? > > Your > > opinions are much appreciated. > > > > > > > > Thank you! > > > > > > > > Jennifer > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > ------------------------------ > > > > Message: 5 > > Date: Thu, 9 Feb 2017 16:19:00 +0000 > > From: Walter Benton > > To: Rene J Buesa , Jennifer > >? ? ? ? > > Cc: "histonet at lists.utsouthwestern.edu" > >? ? ? ? > > Subject: Re: [Histonet] hand staining immunos > > Message-ID: <0952dbba122f4678aa83759b2c98d994 at MAIL01.GCU-MD.local> > > Content-Type: text/plain; charset="utf-8" > > > > http://www.agilent.com/cs/library/technicaloverviews/ > > public/08002_ihc_staining_methods.pdf > > > > > > -----Original Message----- > > From: Rene J Buesa via Histonet [mailto:histonet at lists. > utsouthwestern.edu] > > Sent: Thursday, February 09, 2017 10:51 AM > > To: Jennifer ; histonet at lists.utsouthwestern.edu > > Subject: Re: [Histonet] hand staining immunos > > > > You do not have to "babysit" the procedure for it has well defined/timed > > steps. You just need a timer and check the slides when required. As to > > procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren? > > > >? ? On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet < > > histonet at lists.utsouthwestern.edu> wrote: > > > > > >? Hello, > > > > > > > > My dermatopathologist wants me to start hand staining immunos? for MITF. > I > > would really like some feedback about everyone's experiences/thoughts > with > > manually hand staining immunos and especially with MITF. Pros and cons? I > > am concerned about the amount of time this will take for a tech to > babysit > > the stain and also about the consistency of the quality in the end > result. > > Your opinions are much appreciated. > > > > > > > > Thank you! > > > > > > > > Jennifer > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: The information contained in this electronic > > message is intended solely for the personal and confidential use of the > > designated recipient(s) named above and may contain information that is > > protected from disclosure under applicable law. If you are not the > intended > > recipient, or the employee or agent responsible for delivering it to the > > intended recipient, you are hereby notified that any dissemination, > > distribution or copying of this transmission is strictly prohibited. If > you > > have received this transmission in error, please notify the transmitting > > person/department immediately by email or telephone (410) 581-5881 and > > delete the message without making a copy. > > > > ------------------------------ > > > > Subject: Digest Footer > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > > > End of Histonet Digest, Vol 159, Issue 8 > > **************************************** > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mills at 3scan.com Mon Feb 13 10:54:31 2017 From: mills at 3scan.com (Caroline Miller) Date: Mon, 13 Feb 2017 08:54:31 -0800 Subject: [Histonet] hand staining immunos In-Reply-To: <362094677.3718916.1487000528287@mail.yahoo.com> References: <362094677.3718916.1487000528287@mail.yahoo.com> Message-ID: Agreed! Don't believe the 'single use only' on the coverplates - wash and reuse them. I use tube racks to stand them up to dry, then put the back in the original packaging. after a few years some of them might lose their spring and you will need to replace some at that point. yours, mills On Mon, Feb 13, 2017 at 7:42 AM, Paula Keene Pierce < paula at excaliburpathology.com> wrote: > https://www.fishersci.com/shop/products/7219950/7219950#?keyword=sequenza > > These are the cover plates. > > I have no financial interest in Thermo Fisher, but have used the system > for years. > > > > > Paula Keene Pierce, BS, HTL(ASCP)HT > President > Excalibur Pathology, Inc. > 5830 N Blue Lake Drive > Norman, OK 73069 > PH 405-759-3953 <(405)%20759-3953> > FAX 405-759-7513 <(405)%20759-7513> > www.excaliburpathology.com > > > ------------------------------ > *From:* Caroline Miller via Histonet > *To:* Ranna Mehta > *Cc:* "Histonet at Lists. Edu" > *Sent:* Monday, February 13, 2017 9:06 AM > *Subject:* Re: [Histonet] hand staining immunos > > This is a great system for semi-automated immuno. I find the results a > little cleaner than performing the technique straight on the slide. No need > to buy the whole set up (it is just a glorified timer)- just the racks and > clips are enough! > > https://www.fishersci.com/shop/products/thermo- > scientific-shandon-sequenza-immunostaining-center-2/p-4010433 > > mills > > On Thu, Feb 9, 2017 at 11:32 AM, Ranna Mehta via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hi Jennifer, > > > > How many slides are you planning to stain in routine? Once you will > > understand the basic, it will be a very interesting field. If you like, I > > can help you to walk through process with step by step. send me a message > > through my email.All the best and thanks. > > > > Regards > > Ranna > > > > On Thu, Feb 9, 2017 at 12:00 PM, > utsouthwestern.edu> > > wrote: > > > > > Send Histonet mailing list submissions to > > > histonet at lists.utsouthwestern.edu > > > > > > To subscribe or unsubscribe via the World Wide Web, visit > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > or, via email, send a message with subject or body 'help' to > > > histonet-request at lists.utsouthwestern.edu > > > > > > You can reach the person managing the list at > > > histonet-owner at lists.utsouthwestern.edu > > > > > > When replying, please edit your Subject line so it is more specific > > > than "Re: Contents of Histonet digest..." > > > > > > > > > Today's Topics: > > > > > > 1. Looking for a Histology Workshop (Jessica Riggleman) > > > 2. Re: Looking for a Histology Workshop (Jessica Riggleman) > > > 3. hand staining immunos (Jennifer) > > > 4. Re: hand staining immunos (Rene J Buesa) > > > 5. Re: hand staining immunos (Walter Benton) > > > > > > > > > ---------------------------------------------------------------------- > > > > > > Message: 1 > > > Date: Wed, 8 Feb 2017 18:07:24 +0000 > > > From: Jessica Riggleman > > > To: "histonet at lists.utsouthwestern.edu" > > > > > > Subject: [Histonet] Looking for a Histology Workshop > > > Message-ID: > > > > > > > Content-Type: text/plain; charset=UTF-8 > > > > > > > > > Hi Everyone, > > > > > > I am looking for workshop or class for Histology/Histotechnology that > > > lasts anywhere from 1 week to 3 months in the U.S. > > > Preferably in-person and not online. > > > > > > If you have any suggestions or contacts, please let me know. > > > > > > Thank You, > > > Jessica > > > > > > _____________________________________________________________________ > > > > > > Jessica Riggleman | Research Associate > > > > > > Globus Medical, Inc. > > > Valley Forge Business Center > > > 2560 General Armistead Avenue | Audubon, PA 19403 > > > Ph: (610) 930-1800 ext. 2583 <(610)%20930-1800> | Fax: > > > website: http://www.globusmedical.com | email: > > > jriggleman at globusmedical.com > > > An ISO 13485 Registered Company > > > > > > Confidentiality Note: This email is confidential and intended solely > for > > > the use of the individual to whom it is addressed. If you are not the > > > intended recipient, be advised that you have received this email in > error > > > and that any use, dissemination, forwarding, printing, or copying of > this > > > email is strictly prohibited. If you have received this email in error > > > please contact the sender. Any views or opinions presented are solely > > those > > > of the author and do not necessarily represent those of Globus Medical, > > > Inc. Although this email and any attachments are believed to be free of > > any > > > virus or other defects which might affect any computer or IT system > into > > > which they are received, no responsibility is accepted by Globus > Medical, > > > Inc. for any loss or damage arising in any way from the receipt or use > > > thereof. > > > > > > Disclaimer > > > > > > The information contained in this communication from the sender is > > > confidential. It is intended solely for use by the recipient and others > > > authorized to receive it. If you are not the recipient, you are hereby > > > notified that any disclosure, copying, distribution or taking action in > > > relation of the contents of this information is strictly prohibited and > > may > > > be unlawful. > > > > > > This email has been scanned for viruses and malware, and may have been > > > automatically archived by Mimecast Ltd, an innovator in Software as a > > > Service (SaaS) for business. Providing a safer and more useful place > for > > > your human generated data. Specializing in; Security, archiving and > > > compliance. To find out more visit the Mimecast website. > > > > > > > > > ------------------------------ > > > > > > Message: 2 > > > Date: Wed, 8 Feb 2017 21:33:47 +0000 > > > From: Jessica Riggleman > > > To: Esther C Peters > > > Cc: "histonet at lists.utsouthwestern.edu" > > > > > > Subject: Re: [Histonet] Looking for a Histology Workshop > > > Message-ID: > > > > > > > Content-Type: text/plain; charset=UTF-8 > > > > > > Hi Esther, > > > > > > We are looking for basic training to include study design, sectioning > raw > > > specimens for processing (i.e., how large should my specimen be?), > > > processing techniques (fixation, etc.), special stains, and slide > > reading. > > > This is not for HT/HTL exam preparation. > > > > > > We would prefer non-human tissues, as we typically work with large > sheep > > > and rabbit specimens. Only two students. > > > > > > Thank You, > > > Jessica > > > > > > > > > From: Esther C Peters [mailto:epeters2 at gmu.edu] > > > Sent: Wednesday, February 08, 2017 4:18 PM > > > To: Jessica Riggleman > > > Cc: histonet at lists.utsouthwestern.edu > > > Subject: Re: Looking for a Histology Workshop > > > > > > Is this for human or non-human tissues? How many students? > > > > > > Esther > > > > > > > > > _____________________________________________________________________ > > > > > > Jessica Riggleman | Research Associate > > > > > > Globus Medical, Inc. > > > Valley Forge Business Center > > > 2560 General Armistead Avenue | Audubon, PA 19403 > > > Ph: (610) 930-1800 ext. 2583 <(610)%20930-1800> | Fax: > > > > > > Confidentiality Note: This email is confidential and intended solely > for > > > the use of the individual to whom it is addressed. If you are not the > > > intended recipient, be advised that you have received this email in > error > > > and that any use, dissemination, forwarding, printing, or copying of > this > > > email is strictly prohibited. If you have received this email in error > > > please contact the sender. Any views or opinions presented are solely > > those > > > of the author and do not necessarily represent those of Globus Medical, > > > Inc. Although this email and any attachments are believed to be free of > > any > > > virus or other defects which might affect any computer or IT system > into > > > which they are received, no responsibility is accepted by Globus > Medical, > > > Inc. for any loss or damage arising in any way from the receipt or use > > > thereof. > > > > > > > > > ________________________________________ > > > From: Jessica Riggleman > > > Sent: Wednesday, February 8, 2017 4:12:20 PM > > > To: Esther C Peters > > > Cc: histonet at lists.utsouthwestern.edu > > > Subject: RE: Looking for a Histology Workshop > > > > > > Hi Esther, > > > > > > We are looking for basic training to include study design, sectioning > raw > > > specimens for processing (i.e., how large should my specimen be?), > > > processing techniques (fixation, etc.), special stains, and slide > > reading. > > > This is not for HT/HTL exam preparation. > > > > > > Thank You, > > > Jessica > > > > > > ________________________________________ > > > > > > Jessica Riggleman | Research Associate > > > > > > Globus Medical, Inc. > > > Valley Forge Business Center > > > 2560 General Armistead Avenue | Audubon, PA 19403 > > > Ph: (610) 930-1800 ext. 2583 <(610)%20930-1800> | Fax: > > > An ISO 13485 Registered Company > > > > > > website | map | email > > > > > > > > > > > > > > > > > > Confidentiality Note: This email is confidential and intended solely > for > > > the use of the individual to whom it is addressed. If you are not the > > > intended recipient, be advised that you have received this email in > error > > > and that any use, dissemination, forwarding, printing, or copying of > this > > > email is strictly prohibited. If you have received this email in error > > > please contact the sender. Any views or opinions presented are solely > > those > > > of the author and do not necessarily represent those of Globus Medical, > > > Inc. Although this email and any attachments are believed to be free of > > any > > > virus or other defects which might affect any computer or IT system > into > > > which they are received, no responsibility is accepted by Globus > Medical, > > > Inc. for any loss or damage arising in any way from the receipt or use > > > thereof. > > > From: Esther C Peters [mailto:epeters2 at gmu.edu] > > > Sent: Wednesday, February 08, 2017 3:53 PM > > > To: Jessica Riggleman > > > Subject: Re: Looking for a Histology Workshop > > > > > > Hi Jessica, > > > > > > It might help everyone to know exactly what you want to cover in the > > > workshop or class. Do you need basic training, preparation for the > HT/HTL > > > exam, slide preparation, special stains, or slide reading? What is your > > > goal? > > > > > > Esther > > > > > > Esther C. Peters, Ph.D. > > > George Mason University > > > Fairfax, VA 22030-4444 > > > ________________________________________ > > > From: Jessica Riggleman via Histonet utsouthwestern.edu> > > > Sent: Wednesday, February 8, 2017 1:07:24 PM > > > To: histonet at lists.utsouthwestern.edu > > > Subject: [Histonet] Looking for a Histology Workshop > > > > > > > > > Hi Everyone, > > > > > > I am looking for workshop or class for Histology/Histotechnology that > > > lasts anywhere from 1 week to 3 months in the U.S. > > > Preferably in-person and not online. > > > > > > If you have any suggestions or contacts, please let me know. > > > > > > Thank You, > > > Jessica > > > > > > _____________________________________________________________________ > > > > > > Jessica Riggleman | Research Associate > > > > > > Globus Medical, Inc. > > > Valley Forge Business Center > > > 2560 General Armistead Avenue | Audubon, PA 19403 > > > Ph: (610) 930-1800 ext. 2583 <(610)%20930-1800> | Fax: > > > website: http://www.globusmedical.com | email: > > > jriggleman at globusmedical.com > > > An ISO 13485 Registered Company > > > > > > Confidentiality Note: This email is confidential and intended solely > for > > > the use of the individual to whom it is addressed. If you are not the > > > intended recipient, be advised that you have received this email in > error > > > and that any use, dissemination, forwarding, printing, or copying of > this > > > email is strictly prohibited. If you have received this email in error > > > please contact the sender. Any views or opinions presented are solely > > those > > > of the author and do not necessarily represent those of Globus Medical, > > > Inc. Although this email and any attachments are believed to be free of > > any > > > virus or other defects which might affect any computer or IT system > into > > > which they are received, no responsibility is accepted by Globus > Medical, > > > Inc. for any loss or damage arising in any way from the receipt or use > > > thereof. > > > > > > Disclaimer > > > > > > The information contained in this communication from the sender is > > > confidential. It is intended solely for use by the recipient and others > > > authorized to receive it. If you are not the recipient, you are hereby > > > notified that any disclosure, copying, distribution or taking action in > > > relation of the contents of this information is strictly prohibited and > > may > > > be unlawful. > > > > > > This email has been scanned for viruses and malware, and may have been > > > automatically archived by Mimecast Ltd, an innovator in Software as a > > > Service (SaaS) for business. Providing a safer and more useful place > for > > > your human generated data. Specializing in; Security, archiving and > > > compliance. To find out more visit the Mimecast website. > > > _______________________________________________ > > > Histonet mailing list > > > Histonet at lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > Disclaimer > > > > > > The information contained in this communication from the sender is > > > confidential. It is intended solely for use by the recipient and others > > > authorized to receive it. If you are not the recipient, you are hereby > > > notified that any disclosure, copying, distribution or taking action in > > > relation of the contents of this information is strictly prohibited and > > may > > > be unlawful. > > > > > > This email has been scanned for viruses and malware, and may have been > > > automatically archived by Mimecast Ltd, an innovator in Software as a > > > Service (SaaS) for business. Providing a safer and more useful place > for > > > your human generated data. Specializing in; Security, archiving and > > > compliance. To find out more visit the Mimecast website. > > > > > > ------------------------------ > > > > > > Message: 3 > > > Date: Wed, 8 Feb 2017 16:16:10 -0800 > > > From: "Jennifer" > > > To: > > > Subject: [Histonet] hand staining immunos > > > Message-ID: <000001d28269$b81e82f0$285b88d0$@com> > > > Content-Type: text/plain; charset="us-ascii" > > > > > > Hello, > > > > > > > > > > > > My dermatopathologist wants me to start hand staining immunos for > MITF. > > I > > > would really like some feedback about everyone's experiences/thoughts > > with > > > manually hand staining immunos and especially with MITF. Pros and > cons? I > > > am > > > concerned about the amount of time this will take for a tech to babysit > > the > > > stain and also about the consistency of the quality in the end result. > > > Your > > > opinions are much appreciated. > > > > > > > > > > > > Thank you! > > > > > > > > > > > > Jennifer > > > > > > > > > > > > ------------------------------ > > > > > > Message: 4 > > > Date: Thu, 9 Feb 2017 15:51:22 +0000 (UTC) > > > From: Rene J Buesa > > > To: Jennifer , > > > "histonet at lists.utsouthwestern.edu" > > > > > > Subject: Re: [Histonet] hand staining immunos > > > Message-ID: <267846266.1416224.1486655482834 at mail.yahoo.com> > > > Content-Type: text/plain; charset=UTF-8 > > > > > > You do not have to "babysit" the procedure for it has well > defined/timed > > > steps. You just need a timer and check the slides when required. As to > > > procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren? > > > > > > On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet < > > > histonet at lists.utsouthwestern.edu> wrote: > > > > > > > > > Hello, > > > > > > > > > > > > My dermatopathologist wants me to start hand staining immunos? for > MITF. > > I > > > would really like some feedback about everyone's experiences/thoughts > > with > > > manually hand staining immunos and especially with MITF. Pros and > cons? I > > > am > > > concerned about the amount of time this will take for a tech to babysit > > the > > > stain and also about the consistency of the quality in the end result.? > > > Your > > > opinions are much appreciated. > > > > > > > > > > > > Thank you! > > > > > > > > > > > > Jennifer > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet at lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > > > > ------------------------------ > > > > > > Message: 5 > > > Date: Thu, 9 Feb 2017 16:19:00 +0000 > > > From: Walter Benton > > > To: Rene J Buesa , Jennifer > > > > > > Cc: "histonet at lists.utsouthwestern.edu" > > > > > > Subject: Re: [Histonet] hand staining immunos > > > Message-ID: <0952dbba122f4678aa83759b2c98d994 at MAIL01.GCU-MD.local> > > > Content-Type: text/plain; charset="utf-8" > > > > > > http://www.agilent.com/cs/library/technicaloverviews/ > > > public/08002_ihc_staining_methods.pdf > > > > > > > > > -----Original Message----- > > > From: Rene J Buesa via Histonet [mailto:histonet at lists. > > utsouthwestern.edu] > > > Sent: Thursday, February 09, 2017 10:51 AM > > > To: Jennifer ; histonet at lists.utsouthwestern. > edu > > > Subject: Re: [Histonet] hand staining immunos > > > > > > You do not have to "babysit" the procedure for it has well > defined/timed > > > steps. You just need a timer and check the slides when required. As to > > > procedural "dos & donts" try to get a copy of the DAKO IHC manual.Ren? > > > > > > On Wednesday, February 8, 2017 7:34 PM, Jennifer via Histonet < > > > histonet at lists.utsouthwestern.edu> wrote: > > > > > > > > > Hello, > > > > > > > > > > > > My dermatopathologist wants me to start hand staining immunos for > MITF. > > I > > > would really like some feedback about everyone's experiences/thoughts > > with > > > manually hand staining immunos and especially with MITF. Pros and > cons? I > > > am concerned about the amount of time this will take for a tech to > > babysit > > > the stain and also about the consistency of the quality in the end > > result. > > > Your opinions are much appreciated. > > > > > > > > > > > > Thank you! > > > > > > > > > > > > Jennifer > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet at lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet at lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE: The information contained in this electronic > > > message is intended solely for the personal and confidential use of the > > > designated recipient(s) named above and may contain information that is > > > protected from disclosure under applicable law. If you are not the > > intended > > > recipient, or the employee or agent responsible for delivering it to > the > > > intended recipient, you are hereby notified that any dissemination, > > > distribution or copying of this transmission is strictly prohibited. If > > you > > > have received this transmission in error, please notify the > transmitting > > > person/department immediately by email or telephone (410) 581-5881 and > > > delete the message without making a copy. > > > > > > ------------------------------ > > > > > > Subject: Digest Footer > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet at lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > > > > > End of Histonet Digest, Vol 159, Issue 8 > > > **************************************** > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Caroline Miller (mills) > Director of Histology > 3Scan.com > 415 2187297 <(415)%20218-7297> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From JMacDonald at mtsac.edu Mon Feb 13 12:03:48 2017 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Mon, 13 Feb 2017 10:03:48 -0800 Subject: [Histonet] Clinical Laboratory Educators' Conference Message-ID: This is a great opportunity for those teaching histotechnology. Although many of the workshops are CLS oriented there are many that are about teaching laboratory science in general. There are also workshops about clinical rotation issues. I have gone several years and always bring back great information for our program. Below is a link for the information. http://www.ascls.org/education-meetings/clec From hans at histologistics.com Mon Feb 13 12:47:05 2017 From: hans at histologistics.com (Hans B Snyder) Date: Mon, 13 Feb 2017 13:47:05 -0500 Subject: [Histonet] HPV slides Message-ID: Hello All, Where does everyone get their HPV control slides? I could only find 2 suppliers and they're not one's we are use to dealing with, they don't list prices. Maybe it's not a highly sought after supply? This is the first time we're looking into it, so we have no idea. Any ideas or comments are helpful. Thank you Hans B Snyder Histologistics 151 W Main Street Dudley, MA 01571 Lab - 508-461-7207 C- 508-308-7800 hans at histologistics.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From relia1 at earthlink.net Tue Feb 14 10:57:46 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 14 Feb 2017 11:57:46 -0500 Subject: [Histonet] Relia Histology Opportunities Update 2-14-2017 Happy Valentines' Day. Exciting opportunities, Sweets for the Sweet and a RELIA EXCLUSIVE!! Message-ID: <1ab901d286e3$77dd3690$6797a3b0$@earthlink.net> Hi Histonetters , I hope this is an especially sweet week since today is Valentines? Day. Happy Valentines? Day!!! I have some great histology opportunities to tell you about. Please feel free to take a second and peruse the list kind of the way one peruses an Assortment of chocolates in a heart shaped box! My personal favorites are Chocolate Covered Cherries!! (Especially the ones that are 50% off tomorrow LOL) How about you? All of these are permanent full time positions and our clients offer excellent compensation, benefits, relocation and or sign on bonuses. Here is a list of my current openings!! Let me know if anything looks good!! SPOTLIGHT OPPORTUNITY! A RELIA EXCLUSIVE!!!!! ASCP certified Histology Tech ? Modesto, CA Brand New Facility!! Great team to work with!! New Grads and Experienced Techs Welcome Management: Histology Lab Manager ? San Diego ? A RELIA EXCLUSIVE!!!! Histology Supervisor ? Los Angeles, CA exciting opportunity!! HT/HTL Opportunities: Modesto, CA Austin, TX Tyler, TX Longview, TX Kingsport, TN Scarborough, ME And Many More Other Opportunities Pathologists Assistant ? San Diego, CA Cytotech w/histo experience? Soldotna, AK If you are interested in any of these positions please call or text me on my cell at 407-353-5070 or toll free at 866-607-3542 or e-mail me at relia1 at earthlink.net ? If you would like you can e-mail me your resume and a number where I can reach you at a time that is convenient for you. ? If you are interested in looking into new job opportunities in other areas that are not mentioned above please contact me as well. ? I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember It never hurts to keep an eye open EVEN IF you are happy in your present job. Have a wonderful Valentine?s Day!! Thanks-Pam Thank you, Pam ? 866-607-3542 (866-60RELIA) 407-353-5070-cell ? Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From lblazek at digestivespecialists.com Tue Feb 14 14:01:22 2017 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Tue, 14 Feb 2017 15:01:22 -0500 Subject: [Histonet] Excelsior AS Message-ID: <5A2BD13465E061429D6455C8D6B40E39198A317B99@IBMB7Exchange.digestivespecialists.com> Does anyone have any experience using this processor? Could you share with me the good and/or bad you have experienced? Thanks in advance! :) Linda Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com From PAMarcum at uams.edu Wed Feb 15 09:53:07 2017 From: PAMarcum at uams.edu (Marcum, Pamela A) Date: Wed, 15 Feb 2017 15:53:07 +0000 Subject: [Histonet] AFB Control Question Message-ID: <8c03b2f73b8e4e5593bf93faa406915f@MAIL13M2N1.ad.uams.edu> Good morning, We are attempting to find AFB controls from human sources. We would trade if we have something you need and we have available. Does anyone have blocks they might share or trade without upsetting our HIPPA people on either side. I am really not interested in buying slides as we cut larger blocks down and have controls on with the patient tissue. Also the control slides are general in the middle of the slide and force to use two slides for every test (too many ordering and all want a control) which makes the cost prohibitive. Our pathologists prefer human tissue and most of the companies I talk to either don't know or won't say it is animal origin or human. ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From chiswick at mit.edu Wed Feb 15 10:57:31 2017 From: chiswick at mit.edu (Evan Chiswick) Date: Wed, 15 Feb 2017 16:57:31 +0000 Subject: [Histonet] Acetone freeze substitution vs fixation, and other Cryo questions Message-ID: <162849802C1E1F48AF4304ADD61862115E7CCA71@OC11EXPO26.exchange.mit.edu> Hello Histonet members, I am fairly new to the world of histology, but I have found this listserve to be very helpful. I have a few questions which will be followed by a general description of my project/protocol, in case that is helpful for answering. Question: I have read here and elsewhere of acetone used as a fixative after first drying the section, but other fixatives are used when acetone is used for freeze substitution(e.g. http://jcb.rupress.org/content/jcb/4/5/593.full.pdf). Does freeze substitution with cold acetone also fix the section? I want to avoid air drying the section as I have the impression that will distort the morphology. What happens to freeze-substituted sections when I subsequently immerse then in staining buffer (.e.g. PBS w/BSA & Triton-x). Would that reverse the substitution/fixation? If I want to subsequently store my stained slides for future use/reference, how should I do that? How should freeze substituted slides be stored (pre-staining) Brief description of project. Use highly multiplexed immunofluorescent staining of uterine sections to measure cellular composition, protease activity (amenable to acetone/frozen sections), as well as various other molecular features. Through computational methods, these features will be modeled in conjunction with the spatial and morphological features of the section. Hence, I want a tissue preservation method that preserves enzymatic activity (freezing) and morphological detail (freeze substitution/no air drying??). I am under the impression that air-drying is necessary to ensure attachment of sections to slide. Would the cryojane tape-transfer technique get me around the need for air drying? Brief outline of protocol Snap freeze tissue blocks in isopentane chilled by liquid nitrogen. Section 4um with cryojane, freeze substitute with cold acetone, and store at -80 or liquid nitrogen. Block sections (PBS/BSA Triton-x100, or something similar). Stain with panel of antibodies with unique linkers attached. Wash. Light PFA fixation (~10min) to crosslink antibodies. Iteratively image 3-4 antigens at a time, with cyclic detection incubations, image, dehybridize detection, repeat). Store slide for future use (e.g. additional immunostains, or H&E) (how to store?) Thank you for your time. Sincerely, ~Evan Evan L. Chiswick Postdoctoral Research Associate --- Department of Biological Engineering Massachusetts Institute of Technology Cambridge, MA From Forest.Blankenship at dchstx.org Wed Feb 15 11:19:57 2017 From: Forest.Blankenship at dchstx.org (Forest Blankenship) Date: Wed, 15 Feb 2017 17:19:57 +0000 Subject: [Histonet] AFB Control Message-ID: <8D5A5A0BF8A80F43A6E52872E56F523C013EE6D3@dchmxdb02.driscoll.dch> Hello Pam, It is always recommended to have AFB control and patient tissues run separately in parallel to avoid contamination from the control to the patient tissue. The most economical way to do this is to use a staining tray and use a pipette to do all the steps involved then it is impossible for the bacteria to transfer to the patient slide. Thank you, Forest K. Blankenship, HTL(ASCP) Histology Manager Driscoll Childrens Hospital Corpus Christi, TX Disclaimer: This email and its content are confidential and intended solely for the use of the addressee. Please notify the sender if you have received this email in error or simply delete it. From TNMayer at mdanderson.org Wed Feb 15 12:31:53 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Wed, 15 Feb 2017 18:31:53 +0000 Subject: [Histonet] AFB Control Question Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC88296FA9BF@D1PWPEXMBX08.mdanderson.edu> Pam, I worked with our microbiology department and had them to obtain some positive organisms and with our surgery and morgue for fresh tissue. We then inoculate the tissue and process it. Positive blocks are kept on hand and used. We also pass a copy of the slides back to the microbiology department to keep on file as a good batch. It seems to work for us in the educational setting. We have done afb, gms, and gram. Our next batch will include Cryptococcus, and h. pylori. Hope that helps! Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 The information contained in the e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. ------------------------------ Message: 2 Date: Wed, 15 Feb 2017 15:53:07 +0000 From: "Marcum, Pamela A" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] AFB Control Question Message-ID: <8c03b2f73b8e4e5593bf93faa406915f at MAIL13M2N1.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" Good morning, We are attempting to find AFB controls from human sources. We would trade if we have something you need and we have available. Does anyone have blocks they might share or trade without upsetting our HIPPA people on either side. I am really not interested in buying slides as we cut larger blocks down and have controls on with the patient tissue. Also the control slides are general in the middle of the slide and force to use two slides for every test (too many ordering and all want a control) which makes the cost prohibitive. Our pathologists prefer human tissue and most of the companies I talk to either don't know or won't say it is animal origin or human. ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From abadesuyi at nrh-ok.com Wed Feb 15 15:00:59 2017 From: abadesuyi at nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Wed, 15 Feb 2017 15:00:59 -0600 Subject: [Histonet] AFB Stain Message-ID: <04EE4F75BB5FB246ADB68D69B7460443B98877B3ED@MAIL.nrhnt.nrh-ok.com> Hi, I want to know whether it is compulsory to use two controls (positive & negative) for AFB Stain. Thanks, Banjo Adesuyi ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From nelsonrnch at verizon.net Thu Feb 16 07:12:44 2017 From: nelsonrnch at verizon.net (Patti Nelson - PNP Lab Consultant) Date: Thu, 16 Feb 2017 08:12:44 -0500 Subject: [Histonet] Physician Fee Schedule vs. Clinical Fee Schedule Message-ID: <15a470db1b7-61c1-f138@webprd-m60.mail.aol.com> Hello Everyone I would like to thank you in advance. My question is for all those billers out there. Can you please tell me the difference between the Physician Fee Schedule and the Clinical Fee Schedule. Where can I get a copy of both? I am mostly interested in the CPT codes 88305, 88312, 88312 and 88342. Sincerely, PATTI NELSON H.T.(ASCP) PNH LABORATORY CONSULTANTS SUPERVISOR DGC/ZADEH LABS PO BOX 412 CABAZON, CA. 92230 909-841-9761 nelsonrnch at verizon.net CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender ofthe delivery error by e-mail or you may call 909-841-9761. From criley at dpspa.com Thu Feb 16 07:54:50 2017 From: criley at dpspa.com (Charles Riley) Date: Thu, 16 Feb 2017 08:54:50 -0500 Subject: [Histonet] ANtibody validation records Message-ID: Would anyone be willing to share how the document their new antibody validations when adding a new antibody test to the panel of tests your lab performs. I currently have a basic packet but feel it could be better and wanted to see how everyone else performs/records their testing. The people who were in my position before me failed to keep track of all the new antibodies and now I am trying to redo them all before our upcoming CAP inspection. Any help would great be appreciated -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From LNormington at uwhealth.org Thu Feb 16 08:19:20 2017 From: LNormington at uwhealth.org (Normington Lacy) Date: Thu, 16 Feb 2017 14:19:20 +0000 Subject: [Histonet] ANtibody validation records In-Reply-To: References: Message-ID: <7F7174244DDD1A49BFBEE8A845BC2F2B290D25@UWHC-MBX05.uwhis.hosp.wisc.edu> Here is the document our developmental specialist uses when bring on new antibodies. Hope this helps. Lacy Normington Lacy Normington, HTL(ASCP)CM Manager, Surgical Pathology Lab Services 600 Highland Avenue Madison, WI 53792-2472 Phone: 608-890-9373 -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 16, 2017 7:55 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] ANtibody validation records Would anyone be willing to share how the document their new antibody validations when adding a new antibody test to the panel of tests your lab performs. I currently have a basic packet but feel it could be better and wanted to see how everyone else performs/records their testing. The people who were in my position before me failed to keep track of all the new antibodies and now I am trying to redo them all before our upcoming CAP inspection. Any help would great be appreciated -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Thu Feb 16 09:29:03 2017 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 16 Feb 2017 10:29:03 -0500 Subject: [Histonet] RELIA HOT HISTOLOGY JOB ALERT!!! My phone is ringing off the hook the latest and greatest -- CA, TX, NJ and AZ Message-ID: <082e01d28869$6817cea0$38476be0$@earthlink.net> Hi Histonetters! I don't know about you guys but this week is just flying by for me. Mostly because my phone has been ringing off the hook with new opportunities. I know y'all are busy so I won't take up a lot of your time. Here is the info: ASCP HT/HTL Elizabeth, NJ - Days Austin, TX - Nights Tyler, TX - Days Longview, TX - Days Anaheim, CA - Days Modesto, CA - Nights Management: Irvine, CA San Diego, CA Flagstaff, AZ (Lead Tech) All of my clients offer VERY competitive pay, excellent benefits and relocation assistance. If you are interested in more information on any of these opportunities please contact me. You can reach me via email at relia1 at earthlink.net or toll free at the office at 866-607-3542 or on my cell call or text me at 407-353-5070. I am looking forward to hearing from you! If you know someone who might be interested please pass along the information. If I hire someone you refer to me you will earn a referral bonus. Thanks for taking the time to read my post. Have a great day!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From cjohnson at nmda.nmsu.edu Thu Feb 16 14:13:33 2017 From: cjohnson at nmda.nmsu.edu (Johnson, Carole) Date: Thu, 16 Feb 2017 20:13:33 +0000 Subject: [Histonet] Opening in great veterinary diagnostic lab Message-ID: We have a day shift opening in our lab. Beautiful lab, great equipment, awesome pathologists. If you are interested, please follow this link for more information. http://www.nmda.nmsu.edu/humanresources/ Carole L. Johnson, HT(ASCP)cm, QIHC New Mexico Department of Agriculture Veterinary Diagnostic Services 1101 Camino de Salud, NE Albuquerque, NM 87101 505.383.9299 Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From cebass at buffalo.edu Fri Feb 17 00:56:25 2017 From: cebass at buffalo.edu (Bass, Caroline) Date: Fri, 17 Feb 2017 06:56:25 +0000 Subject: [Histonet] penetration problem with floating brain sections Message-ID: Hello All, I?m doing some IHC with DAB on formaldehyde fixed rat brain floating sections, either 35 of 50 um thick. Usually I get fine staining of TH for example, but now I?m going after a neuron that's a bit sparse, and I noticed I have a couple of darkly stained cells. But I can see dozens of ?ghost cells?, these appear to be very lightly stained in the right place. My guess is that the antibody isn?t penetrating fully, and so cells on the face of the sections are staining darkly, and some on the interior are just barely been stained. Any suggestions on improving penetrance? I usually have primary on overnight, shaking in the cold room, and secondary at RT for 2 hours. I was thinking of extending the primary to room temperature and maybe upping the triton-X concentration. Any other suggestions? Secondly, I noticed were getting some cracks in the tissue. We usually mount the DAB stained sections on some sort of tissue bond slide, and then let it dry overnight before dilipidating and coverslipping with permeant. My best guess is that sometime during the drying or coverslipping the tissue pulls away slight, resulting in these smallish cracks. Anyway, any advice on handling these two issues would be greatly appreciated. Thanks, Caroline Bass University at Buffalo From Jcox90 at yahoo.com Sat Feb 18 14:02:51 2017 From: Jcox90 at yahoo.com (Jill Cox) Date: Sat, 18 Feb 2017 13:02:51 -0700 Subject: [Histonet] Colloidal iron leaching References: <7CE26C8D-AEB4-428C-BBB3-63DFBBDF9CA0@cox.net> Message-ID: >> Hello netters, >> We have had a problem with our colloidal iron leaching for about a year now. We have changed all reagents and tried different kits. Has anyone else had this problem? I cannot figure out what it is it's making me crazy! We go directly into clean 100% alcohol then clearing. Any advice would be greatly appreciated, thanks in advance! >> >> Sent from my iPhone From hans at histologistics.com Sun Feb 19 15:24:02 2017 From: hans at histologistics.com (Hans Snyder (Histologistics)) Date: Sun, 19 Feb 2017 16:24:02 -0500 Subject: [Histonet] Histonet Digest, Vol 159, Issue 16 In-Reply-To: References: Message-ID: <704FFEDC-70E6-4CDA-895D-081F37F74311@histologistics.com> What protocol or kit are you using? Hans B Snyder President Histologistics Inc 151 W Main St Lowr Dudley, MA 01571 508-308-7800 hans at histologistics.com > On Feb 19, 2017, at 13:00, histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Colloidal iron leaching (Jill Cox) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 18 Feb 2017 13:02:51 -0700 > From: Jill Cox > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Colloidal iron leaching > Message-ID: > Content-Type: text/plain; charset=us-ascii > > >>> Hello netters, >>> We have had a problem with our colloidal iron leaching for about a year now. We have changed all reagents and tried different kits. Has anyone else had this problem? I cannot figure out what it is it's making me crazy! We go directly into clean 100% alcohol then clearing. Any advice would be greatly appreciated, thanks in advance! >>> >>> Sent from my iPhone > > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 159, Issue 16 > ***************************************** From SteveM at mcclainlab.com Mon Feb 20 03:25:32 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Mon, 20 Feb 2017 09:25:32 +0000 Subject: [Histonet] Histonet Digest, Vol 159, Issue 16 Colloidal iron leaching In-Reply-To: References: Message-ID: After checking reagents and glassware, look for other sources of iron contamination, e.g. Metal forceps, steel staining racks, water bath. Inadequate rinsing after the colloidal iron step - increase wash times, using DI Check concentration of working reagent. Decrease the time in colloidal iron working reagent to 5 min. Hales method specified 5-20 min. > Steve A. McClain, MD > >>> Hello netters, >>> We have had a problem with our colloidal iron leaching for about a year now. We have changed all reagents and tried different kits. Has anyone else had this problem? I cannot figure out what it is it's making me crazy! We go directly into clean 100% alcohol then clearing. Any advice would be greatly appreciated, thanks in advance! >>> >>> Sent from my iPhone From jclark at pcnm.com Mon Feb 20 10:04:13 2017 From: jclark at pcnm.com (Joanne Clark) Date: Mon, 20 Feb 2017 16:04:13 +0000 Subject: [Histonet] p16 Message-ID: Good Morning Histonetters, I am wondering how all of you using p16 from Roche on a Leica staining system are going to adapt to the changes coming from Roche. Are you going to pay the exorbitant price for the new kit and list the test as an LDT in your reports, or are you going to source one of the ASR antibodies out there and use that route? I just want to be sure I can re work a research antibody and report it as an ASR test in our reports without CAP giving us a hard time about it. Thanks for the feedback. Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From jshelley at sbpdiscovery.org Tue Feb 21 10:26:05 2017 From: jshelley at sbpdiscovery.org (John Shelley) Date: Tue, 21 Feb 2017 16:26:05 +0000 Subject: [Histonet] Frog tissue Message-ID: Good Morning Histonetters, My daughter's class dissected frogs and her teacher gave me a frog to dissect and make histological slides and blocks for her. I have taken a bunch of tissue and not too sure how well preserved the tissue may be but I thought I would give it a try. I am sure that someone out on the Histonet has encountered such tissue and would like some help with what a good processing schedule would be. I tried to cut everything between 3-4 mm but may have some a slight bit thicker. I have all internal organs like heart, lung, kidney, stomach, intestines, female reproductive system and some eggs. I have outer skin, an eye and both front and back hands and feet for a better term, along with tongue and upper jaw of mouth. I have decaled some bone. So as you can see I have an array of tissue types that may or may not require different schedules on the tissue processor and any help would be greatly appreciated. I thank you in advance! Kind Regards! John J Shelley From koellingr at comcast.net Tue Feb 21 11:09:58 2017 From: koellingr at comcast.net (koellingr at comcast.net) Date: Tue, 21 Feb 2017 17:09:58 +0000 (UTC) Subject: [Histonet] Frog tissue In-Reply-To: References: Message-ID: <1480766809.117461690.1487696998697.JavaMail.zimbra@comcast.net> Hello, For years and years in past, I did the very same thing for a middle school science teacher before, during and after son was there. Don't know what level this is at so unless all the kids are interested in histology, I've found no need to go too far with this (are they really going to learn about gut and lung and heart histology) based on the slides? I collected a fresh frog from teacher and fixed in 70% isopropyl from drug store. Worked great with just "normal histology processing" for level they were at. One really neat thing to look at is two sections of skin, embedded on edge. From a non-spotted area and a spotted skin area. The kids can really appreciate, at microscopic level and referring back to macro, the distribution of melanophores that cause the spots. I was really impressed when I first saw it. All the individual cells with pigment. Ray Raymond Koelling Lecturer, University of Washington School of Medicine, WWAMI, Spokane, WA ----- Original Message ----- From: "John Shelley via Histonet" To: histonet at lists.utsouthwestern.edu Sent: Tuesday, February 21, 2017 8:26:05 AM Subject: [Histonet] Frog tissue Good Morning Histonetters, My daughter's class dissected frogs and her teacher gave me a frog to dissect and make histological slides and blocks for her. I have taken a bunch of tissue and not too sure how well preserved the tissue may be but I thought I would give it a try. I am sure that someone out on the Histonet has encountered such tissue and would like some help with what a good processing schedule would be. I tried to cut everything between 3-4 mm but may have some a slight bit thicker. I have all internal organs like heart, lung, kidney, stomach, intestines, female reproductive system and some eggs. I have outer skin, an eye and both front and back hands and feet for a better term, along with tongue and upper jaw of mouth. I have decaled some bone. So as you can see I have an array of tissue types that may or may not require different schedules on the tissue processor and any help would be greatly appreciated. I thank you in advance! Kind Regards! John J Shelley _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdwyatt at vet.k-state.edu Tue Feb 21 12:36:18 2017 From: kdwyatt at vet.k-state.edu (Kristina Wyatt) Date: Tue, 21 Feb 2017 18:36:18 +0000 Subject: [Histonet] Histology job Message-ID: Kansas State Veterinary Diagnostic Lab is hiring! To apply: http://careers.k-state.edu/cw/en-us/job/500564/research-assistant-veterinary-diagnostic-laboratory General Description of Position: The Veterinary Diagnostic Laboratory with the College of Veterinary Medicine seeks a full time term Research Assistant. This position will cover histology duties related to service and research. Duties will included trimming (grossing) of fixed biopsy and necropsy tissues, embedding tissues in paraffin wax, cutting tissues on a microtome and preparing glass slides of the tissues in accordance with those QA/QC guidelines of an AAVLD accredited laboratory. Also, routine filing of slides, tissue blocks, data entry, operating and maintaining automated histology equipment, immunohistochemical staining, disposing of tissues and updating standard operating procedures. Job Description: * Prepare tissue sections for histopathology: diagnostic and research samples (glass slides). * Embed processed tissues in paraffin wax, cut tissue sections on microtome and prepare glass slides, stain tissue sections using appropriate stains for routine histopathology (H&E) and special stains as requested, coverslip slides (all steps of producing glass slides from beginning to end). Perform special stains to determine the presence of fungi, bacteria, mycobacteria or other infectious agents/abnormal cellular morphology in formalin fixed tissue sections. * Maintain embedding station, autostainers and automatic coverslipper and tissue processors. * Trim biopsy, necropsy and research tissues for histopathology. This task includes trimming samples for margin evaluation when necessary, documenting tissue/biopsy samples received, and providing written images/drawings/photographs for use by the pathologist reading the slides. Includes writing on or printing cassettes with the semi-automated cassette printer, cleaning and maintaining the trim areas and associated equipment. * Maintain trim room, maintaining inventory of trimming supplies. * Assist in the histopathology and immunohistochemistry areas. * Assist in the process of immunohistochemical staining for prion diseases (scrapie, chronic wasting disease), very limited fluorescent antibody testing and limited operation of digital pathology projects will be required. * Assist in writing stand operating procedures. Required Minimum Qualifications: Bachelor?s degree in microbiology, biology, chemistry, animal science or other related fields. Eight years of histology laboratory experience may be substituted for education as determined relevant by the agency. One year of wet laboratory experience. Preferred Qualifications: Certification in histotechnology, ASCP [histotechnician (HT) or histotechnologist (HTL)]; histology experiences. Familiarity with laboratory tests and procedures, working knowledge of chemistry, familiarity with animal diseases, interest in animals, a good team player and knowledge of safe laboratory practices. Special Instructions to Applicants: To apply, please submit an application along with a cover letter, resume and contact information for three professional references. Screening of Applications Begins: Screening of applications begins immediately and will continue until the position is filled. Equal Employment Opportunity: Kansas State University is an Equal Opportunity Employer of individuals with disabilities and protected veterans and actively seeks diversity among its employees. Equal Employment Opportunity is the Law. Background Screening Statement: In connection with your application for employment, Kansas State University may procure a Background Screen on you as part of the process of considering your candidacy as an employee. Kristina Wyatt, M.A. Supervisor: Histopathology/Immunohistochemistry L216 Mosier Hall Kansas State Veterinary Diagnostic Laboratory 785-532-4464 kdwyatt at vet.k-state.edu From tgenade at gmail.com Tue Feb 21 12:52:34 2017 From: tgenade at gmail.com (Tyrone Genade) Date: Tue, 21 Feb 2017 12:52:34 -0600 Subject: [Histonet] FYI: DAB vs 88% formic acid Message-ID: Hello, In case you are curious DAB on IHC sections is resistant to 88% formic acid. If you want to know why I would put a DAB stained section in formic acid, I have conformation specific antibody that I want to use in a double-labelling experiment using an antibody needing formic acid epitope retrieval... Incidentally, having trouble matching antibodies for double labelling? This might be useful: Antibody Elution Method for Multiple Immunohistochemistry on Primary Antibodies Raised in the Same Species and of the Same Subtype http://journals.sagepub.com/doi/10.1369/jhc.2009.953240 I haven't tried this method yet but I will... bye -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From jdhannasch at gmail.com Tue Feb 21 15:10:18 2017 From: jdhannasch at gmail.com (jdhannasch at gmail.com) Date: Tue, 21 Feb 2017 14:10:18 -0700 Subject: [Histonet] ASCP HTL study guide Message-ID: <4500EF9B-F391-4168-B5DC-B29132C7C32A@gmail.com> I am starting to study for the American Society for Clinical Pathology (ASCP) Histotechnology (HTL) certification. ASCP's website gives an extreme vague guide of what to study and their vague study guide requires multiple books. I am currently an ASCP histotechnician (HT) so I'm aware of how hard certification is. Does anyone have any pointer on study materials or guides for the HTL certification? Thanks in advance for any advice even if it's a small piece of advice. From tabbott at pennstatehealth.psu.edu Wed Feb 22 06:34:40 2017 From: tabbott at pennstatehealth.psu.edu (Abbott, Tanya) Date: Wed, 22 Feb 2017 12:34:40 +0000 Subject: [Histonet] Temporary Histotech position Message-ID: <4c9535d6783e4e40847b142766373985@EXCHSRVR12.mshmc.local> I am in need of a temporary Histotechnologist from approximately March 3 for 8 to 10 weeks, any interest, please contact me, thanks! Tanya Tanya G. Abbott, HT (ASCP)CM, RT(CSMLS) Pathology Manager Penn State Health St. Joseph Pathology Department (o) 610-378-2635 (f) 670-898-5871 tabbott at pennstatehealth.psu.edu Penn State Health St. Joseph | The Future of Healthcare From lmarie08 at uga.edu Wed Feb 22 12:59:35 2017 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Wed, 22 Feb 2017 18:59:35 +0000 Subject: [Histonet] review Message-ID: Hi all in histoworld, Has anyone out there used the Thermo Autostainer 360 system before? If so, what are your experiences with it? Good, bad, whatever. Thanks! L From garethdavisyuma at gmail.com Wed Feb 22 14:23:14 2017 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Wed, 22 Feb 2017 13:23:14 -0700 Subject: [Histonet] Recycled reagents in VIP processor Message-ID: Hi, I was always told not to use recycled reagents, i.e. Alcohol and Xylene, in processors. I am using a VIP 300, refurbished, and I would rather not use recycled reagents in it. But, during the last CAP inspection they suggested I use the recycled to save money. And now my administration wants to cut cost. Just wanted to know what labs were doing. Thanks, -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From NMargaryan at luriechildrens.org Wed Feb 22 14:32:15 2017 From: NMargaryan at luriechildrens.org (Margaryan, Naira) Date: Wed, 22 Feb 2017 20:32:15 +0000 Subject: [Histonet] Recycled reagents in VIP processor In-Reply-To: References: Message-ID: <34B2EDA118548A4EB35D6E650345BA6469BE016D@SV-EX08.childrensmemorial.org> WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY.... Thanks, Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) ________________________________________ From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, February 22, 2017 2:23 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Recycled reagents in VIP processor Hi, I was always told not to use recycled reagents, i.e. Alcohol and Xylene, in processors. I am using a VIP 300, refurbished, and I would rather not use recycled reagents in it. But, during the last CAP inspection they suggested I use the recycled to save money. And now my administration wants to cut cost. Just wanted to know what labs were doing. Thanks, -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria at gmail.com Wed Feb 22 14:40:30 2017 From: bakevictoria at gmail.com (Victoria Baker) Date: Wed, 22 Feb 2017 12:40:30 -0800 Subject: [Histonet] Recycled reagents in VIP processor In-Reply-To: References: Message-ID: We routinely recycle xylene and alcohol on a CBG recycler without issues. We also run ER PR HER2 breast panel, ISH and routine IHC Vikki Baker On Feb 22, 2017 3:37 PM, "Gareth Davis via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > Hi, > I was always told not to use recycled reagents, i.e. Alcohol and Xylene, in > processors. I am using a VIP 300, refurbished, and I would rather not use > recycled reagents in it. But, during the last CAP inspection they > suggested I use the recycled to save money. And now my administration > wants to cut cost. Just wanted to know what labs were doing. > Thanks, > > > -- > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm > Yuma Gastroenterology > Yuma, AZ 85364 > 928-248-5259 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From brannon at alliedsearchpartners.com Wed Feb 22 15:23:59 2017 From: brannon at alliedsearchpartners.com (Brannon Owens) Date: Wed, 22 Feb 2017 21:23:59 +0000 Subject: [Histonet] Histology Supervisor (3rd shift) job opening in southern CA - $5k sign on Message-ID: Hello Histonet ? We are currently seeking an experienced and qualified Histology Supervisor to lead an overnight shift in a southern California laboratory. A full job description with all available information can be found on our website. Relocation assistance IS offered as is a $5k sign on bonus and competitive salary and benefits package. To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers/ Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 Direct Line: 407.413.9421 F: 888.388.7572 From liz at premierlab.com Wed Feb 22 15:29:49 2017 From: liz at premierlab.com (Elizabeth Chlipala) Date: Wed, 22 Feb 2017 14:29:49 -0700 Subject: [Histonet] Recycled reagents in VIP processor In-Reply-To: <34B2EDA118548A4EB35D6E650345BA6469BE016D@SV-EX08.childrensmemorial.org> References: , <34B2EDA118548A4EB35D6E650345BA6469BE016D@SV-EX08.childrensmemorial.org> Message-ID: <14E2C6176416974295479C64A11CB9AE0302BCE42BA1@SBS2K8.premierlab.local> We have been recycling for years now and have not had any adverse effects. We have a VIP 3 and a VIP 6. We do use fresh xylene for the cleaning cycle and we only recycle our 95 and absolute alcohols along with both xylene and propar. We use a fractional distillation unit from CBG. We have a specific procedure that we follow so that each xylene cycle is issued a lot number and we test all alcohol with a hydrometer and adjust to temperature prior to using it to prepare, 50, 70, 80 and 95% solutions, all of those when prepared are given lot numbers. If you are NSH members and on the BLOCK my SOP's for reagent control, etc. are in the library. We have decreased our costs for both reagents and waste disposal to me its works quite well if you manage it properly. We have moved from a small quantity generator to a conditionally exempt small quantity generator since we started recycling. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz at premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: Margaryan, Naira via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, February 22, 2017 1:32 PM To: Gareth Davis; histonet at lists.utsouthwestern.edu Cc: naira.margaryan at hsc.wvu.edu Subject: Re: [Histonet] Recycled reagents in VIP processor WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY.... Thanks, Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) ________________________________________ From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, February 22, 2017 2:23 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Recycled reagents in VIP processor Hi, I was always told not to use recycled reagents, i.e. Alcohol and Xylene, in processors. I am using a VIP 300, refurbished, and I would rather not use recycled reagents in it. But, during the last CAP inspection they suggested I use the recycled to save money. And now my administration wants to cut cost. Just wanted to know what labs were doing. Thanks, -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garethdavisyuma at gmail.com Wed Feb 22 15:42:11 2017 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Wed, 22 Feb 2017 14:42:11 -0700 Subject: [Histonet] Great input on recycled reagents Message-ID: Thank you so much. I will start using them on the processor, but start slowly. Then I can monitor to make sure no problems occur. I really appreciate all the responses. Thanks, -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From bakevictoria at gmail.com Wed Feb 22 16:00:14 2017 From: bakevictoria at gmail.com (Victoria Baker) Date: Wed, 22 Feb 2017 14:00:14 -0800 Subject: [Histonet] Great input on recycled reagents In-Reply-To: References: Message-ID: By using a hygrometer (did I spell that right?) you should be able to see the purity of the alcohol. We usually get between 97 to 98 percent. We do use fresh absolute alcohol always in the processor. We start with 80, 95 all recycled. On Feb 22, 2017 4:55 PM, "Gareth Davis via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > Thank you so much. I will start using them on the processor, but start > slowly. Then I can monitor to make sure no problems occur. I really > appreciate all the responses. > Thanks, > > -- > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm > Yuma Gastroenterology > Yuma, AZ 85364 > 928-248-5259 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tkngflght at yahoo.com Wed Feb 22 17:20:39 2017 From: tkngflght at yahoo.com (Cheryl) Date: Wed, 22 Feb 2017 23:20:39 +0000 (UTC) Subject: [Histonet] Used Olympus and Nikon microscopes (vendor responses welcome) References: <2010754236.2872926.1487805639842.ref@mail.yahoo.com> Message-ID: <2010754236.2872926.1487805639842@mail.yahoo.com> Hi guys- Looking for a couple of very clean used Nikon or Olympus pathology light microscopes. ?I have a base setup-- need three total. Vendor responses welcome--? Thanks!?Cheryl Kerry, HT(ASCP) Full Staff Inc. ? admin at fullstaff.org?? https://www.facebook.com/TheHistologyCompany/ From relia1 at earthlink.net Thu Feb 23 10:08:11 2017 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 23 Feb 2017 11:08:11 -0500 Subject: [Histonet] Exciting Day Shift Histology Position in NJ! Message-ID: <015301d28def$07d07d90$177178b0$@earthlink.net> Hi Histonetters, How are you? I hope you are having a great week! I was hoping you might be able to help me.? I am presently working with a client near Elizabeth, NJ in need of a histotech.? This is a full time permanent DAY shift position.? ASCP certification is required and 2 years of experience are preferred.? The position involves performing routine histology ? cutting, embedding and special stains.? IHC and or grossing experience are plusses.?? The help I need from you is do you know anyone that might be interested in hearing about this opportunity?? If so could you please forward my e-mail to them? (remember if I place someone you refer to me you will earn a 250.00 referral fee.) If you are interested in this position please contact me ASAP toll free at 866-607-3542, cell/text 407-353-5070 or via email at relia1 at earthlink.net??? If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide.? I will keep your resume confidential and I won?t release it to anyone without your permission. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From james.fortune at unitypoint.org Thu Feb 23 11:27:14 2017 From: james.fortune at unitypoint.org (Fortune, James A.) Date: Thu, 23 Feb 2017 17:27:14 +0000 Subject: [Histonet] External alarm system for VIP 6 Message-ID: Hello all, We are looking to purchase an external alarm system for our 2 vip 6 processors. I called Sakura to see if they sold them or if they had any recommendations as far as which ones are compatible with the VIP and got nowhere. So I am asking all of the histonet world for any recommendations on external alarms you have used or purchased. Thanks in advance! James (Andy) Fortune Histology Tech Specialist Meriter Health Services Madison, WI This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. sections 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Richard.Cartun at hhchealth.org Thu Feb 23 11:31:54 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Thu, 23 Feb 2017 17:31:54 +0000 Subject: [Histonet] BRG1 IHC Testing Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E953F4038@HHCEXCHMB03.hhcsystem.org> Is anyone doing clinical IHC testing for BRG1/SMARCA4? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From VKurth at uwhealth.org Thu Feb 23 11:41:27 2017 From: VKurth at uwhealth.org (Kurth Virginia L.) Date: Thu, 23 Feb 2017 17:41:27 +0000 Subject: [Histonet] ATRX Ab Recommendation Message-ID: Hi Dave Did you ever receive any information on the antibody ATRX? Our neuropathologist is interested. Thanks Ginny Kurth vkurth at uwhealth.org Surgical Pathology Developmental Specialist University Hospital Wisconsin From VKurth at uwhealth.org Thu Feb 23 11:42:29 2017 From: VKurth at uwhealth.org (Kurth Virginia L.) Date: Thu, 23 Feb 2017 17:42:29 +0000 Subject: [Histonet] ATRX Antibody Message-ID: Hi Pam I was wondering if any reached out to you about ATRX? Our neuropathologist is asking about it. Thanks Ginny Kurth vkurth at uwhealth.org Surgical Pathology Developmental Specialist University Hospital Wisconsin From diane.satterfield at duke.edu Thu Feb 23 11:42:57 2017 From: diane.satterfield at duke.edu (Diane Satterfield) Date: Thu, 23 Feb 2017 17:42:57 +0000 Subject: [Histonet] Alcoholic Eosin-Phloxine in tissue processing Message-ID: I am in the process of setting up a Leica TP1020 processor. I was looking at someone else's SOP where in a 100% EtOH steps they add alcoholic eosin-phloxine. Can someone explain to me what this does and do I have to add this to one of the 100% EtOH steps in the processing. Thanks Diane From angie at ka-recruiting.com Thu Feb 23 12:19:21 2017 From: angie at ka-recruiting.com (Angie Laparidis) Date: Thu, 23 Feb 2017 13:19:21 -0500 Subject: [Histonet] 1st shift and 3rd shift openings in NC Message-ID: Hello Histonet users, I wanted to fill you in on two fantastic new openings in Greensboro, NC that just came available! A private laboratory is looking for a 1st shift tech and a 3rd shift tech to fill their Full-time needs. They are offering competitive packages and are in a great location. In other words...*these will not be open very long*. Please feel free to send over an updated resume if you are interested and check out some of my other openings below. Look forward to hearing from you! AR - Little Rock- Histotech 1st shift, starting at either 4:30AM, or 5AM AR - Little Rock -1st shift, Outreach & Dermatapothology lab GA - Atlanta - Histotech (3rd shift (11 pm to 7 am) (Hold) IL - Freeport -Histotech (part time, 6am-2:30pm) IL - Chicago area Histotechnician (Midnights) NC - Raleigh- IHC Tech - 2nd Shift *NC - Greensboro - Histotech * NM - Las Cruces -Assistant Histology Supervisor NY - New York City -Histotech (day shift) NY - Long Island- Histotech (NYS license required)(Monday - Friday, 5am-1:30pm) OH - Columbus- Histotech VA - Richmond- Histotechnologist (days) WI - Green Bay area- HT/ HTL (2nd & 3rd shifts) Sincerely, *Angie Laparidis*Healthcare Recruiter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South, Boston, MA, 02109 W: 617.746-2744 (*please note this is a new number*) F: (617) 507-8009 Angie at ka-recruiting.com Our openings are updated daily at www.ka-recruiting.com From bcdukes at lexhealth.org Thu Feb 23 12:21:43 2017 From: bcdukes at lexhealth.org (Blake Taylor) Date: Thu, 23 Feb 2017 18:21:43 +0000 Subject: [Histonet] Xylene Free Message-ID: Our hospital has asked us to investigate going xylene free. Has anyone recently made a switch to a xylene alternative and have you been happy with your results? We currently have 4 VIP processors and two Leica auto stainers. Thanks for your input. Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes at lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use to the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. From SteveM at mcclainlab.com Thu Feb 23 12:22:46 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Thu, 23 Feb 2017 18:22:46 +0000 Subject: [Histonet] Histonet Digest, Vol 159, Issue 20Subject: Recycled reagents in VIP processor In-Reply-To: References: Message-ID: <6C6A869D-A683-48D3-8067-EB21A45880F7@mcclainlab.com> We only use recycled reagents (except 100%) on processor. We only use Fresh reagents on stainer and coverslipper. Steve A. McClain, MD From jclark at pcnm.com Thu Feb 23 12:36:25 2017 From: jclark at pcnm.com (Joanne Clark) Date: Thu, 23 Feb 2017 18:36:25 +0000 Subject: [Histonet] Histonet Digest, Vol 159, Issue 20 In-Reply-To: References: Message-ID: <6532fc545b0143a9aab0c571d553705d@E15MADAG-D06N04.sh11.lan> Hi James, most processors come with wiring for hookup to an alarm system. We just purchased one from Radio shack, hooked the external wiring from the processors into it, hung it on the wall behind the units,then programmed the phone numbers to call into the alarm system. It's fairly old now, but it's a Radio Shack Security Auto Dialer. Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Message: 11 Date: Thu, 23 Feb 2017 17:27:14 +0000 From: "Fortune, James A." To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] External alarm system for VIP 6 Message-ID: Content-Type: text/plain; charset="us-ascii" Hello all, We are looking to purchase an external alarm system for our 2 vip 6 processors. I called Sakura to see if they sold them or if they had any recommendations as far as which ones are compatible with the VIP and got nowhere. So I am asking all of the histonet world for any recommendations on external alarms you have used or purchased. Thanks in advance! James (Andy) Fortune Histology Tech Specialist Meriter Health Services Madison, WI This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. sections 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From jclark at pcnm.com Thu Feb 23 12:44:54 2017 From: jclark at pcnm.com (Joanne Clark) Date: Thu, 23 Feb 2017 18:44:54 +0000 Subject: [Histonet] GLX Linnistainer Message-ID: <613ad7984e2b4428965263d30774c9e4@E15MADAG-D06N04.sh11.lan> Greetings Histonetters, we recently purchased a refurbished GLX Linnistainer that has an 8 minute stain time. I was wondering if any of you out there use the same instrument and can give me some insight on your reagent sequence and what type of Hematoxylin and Eosin your using. Thank you! Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From mwerdler at gmail.com Thu Feb 23 13:03:58 2017 From: mwerdler at gmail.com (Mca Werdler) Date: Thu, 23 Feb 2017 13:03:58 -0600 Subject: [Histonet] Xylene Free In-Reply-To: References: Message-ID: I am also interested in that i run a small lab and would love to go xylene free! 2017-02-23 12:21 GMT-06:00 Blake Taylor via Histonet < histonet at lists.utsouthwestern.edu>: > Our hospital has asked us to investigate going xylene free. Has anyone > recently made a switch to a xylene alternative and have you been happy with > your results? We currently have 4 VIP processors and two Leica auto > stainers. > > Thanks for your input. > > Blake Taylor > Surgical Pathology Supervisor > Lexington Medical Center > 803-936-8214 > bcdukes at lexhealth.org > > PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments > are confidential property of the sender. The information is intended only > for the use to the person to whom it was addressed. Any other interception, > copying, accessing, or disclosure of this message is prohibited. The sender > takes no responsibility for any unauthorized reliance on this message. If > you have received this message in error, please immediately notify the > sender and purge the message you received. Do not forward this message > without permission. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Linda.Margraf at cookchildrens.org Thu Feb 23 13:17:11 2017 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Thu, 23 Feb 2017 19:17:11 +0000 Subject: [Histonet] BRG1 IHC Message-ID: <2b5982ccf2794067ad850beff0a1c3bb@MBX10.CCHCS.LDAP> Richard, Propath Labs in Dallas TX offers BRG1 IHC. I have used it a few times now on brain tumor cases and it seems great. We use their immunohistochemistry services all the time. Propath.com is their website. Linda M Histonet administrator ------------------------------ Message: 12 Date: Thu, 23 Feb 2017 17:31:54 +0000 From: "Cartun, Richard" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] BRG1 IHC Testing Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E953F4038 at HHCEXCHMB03.hhcsystem.org> Content-Type: text/plain; charset="us-ascii" Is anyone doing clinical IHC testing for BRG1/SMARCA4? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ********************************* From tbraud at holyredeemer.com Thu Feb 23 15:07:18 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 23 Feb 2017 21:07:18 +0000 Subject: [Histonet] out with the old Message-ID: <48E053DDF6CE074DB6A7414BA05403F81248A6@HRHEX03-HOS.holyredeemer.local> We have just replaced our 2 old tissue processors and have one very old (1989) VIP3000 floor model, the one with the magnets. It is in working condition, but will not hold the battery charge for the backup battery. We have been using it with a UPS, for 6 runs every week. If anyone is interested in it for parts, or for a cheap processor, please contact me. Now the question: We used to use Eosin in our 1st absolute alcohol to tint our biopsies, but it is not recommended for the new processors. For those of you in a similar position, what do you use, if anything, to help visualize tiny biopsies? Thanks in advance for any suggestions. Sincerely, Terri Braud Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From SteveM at mcclainlab.com Thu Feb 23 16:44:28 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Thu, 23 Feb 2017 22:44:28 +0000 Subject: [Histonet] Histonet Digest, Vol 159, Issue 20Subject: simple tests for xylene contamination and alcohol conc. in Recycled alcohol In-Reply-To: <6C6A869D-A683-48D3-8067-EB21A45880F7@mcclainlab.com> References: , <6C6A869D-A683-48D3-8067-EB21A45880F7@mcclainlab.com> Message-ID: Eventually your recycler retort will become contaminated. When you suspect your recycled alcohol product is contaminated by xylene, here is one fairly sensitive yet simple test. Use a 100 ml glass graduated cylinder Add 50ml recycled 95% Add 50ml water. Shake well. When xylene is present it will form a thin layer on top. Cover w parafilm and let sit over night. Each ml of xylene=1% A second test one can do is measure specific gravity by hydrometer in another 50ml sample and graduated cylinder. Specific Gravity should be about 0.82 to 0.85. Steve A. McClain, MD > On Feb 23, 2017, at 13:22, Steve McClain wrote: > > We only use recycled reagents (except 100%) on processor. > We only use Fresh reagents on stainer and coverslipper. > Steve A. McClain, MD From travers.3 at osu.edu Sat Feb 25 08:22:29 2017 From: travers.3 at osu.edu (Travers, Susan) Date: Sat, 25 Feb 2017 14:22:29 +0000 Subject: [Histonet] ultraclone RA95101 PGP 9.5 aby source or replacement Message-ID: Hello, Does anyone know a good replacement for the anti PGP 9.5 aby offered by Ultraclone. This seems to have been the most common source for this aby for many years but I cannot find a website in order to buy it so assume it's no longer available. Any advice would be greatly appreciated. We would like to stain nerve fibers in mouse and human tongue. Thanks! st Susan Travers Professor Division of Biosciences College of Dentistry The Ohio State University 305 West 12th Avenue Columbus, Ohio 43210 614-292-6366 (office) 614-457-6945 (fax) 614-361-0800 (cell) From vkline650 at comcast.net Sat Feb 25 16:00:45 2017 From: vkline650 at comcast.net (Victoria Kline) Date: Sat, 25 Feb 2017 17:00:45 -0500 Subject: [Histonet] MOHS embedding Message-ID: <059A2C98D66A44A7AC2C80DC844F0F3A@vicPC> Hello! I've been a histotech for several years and have recently joined a dermatology practice that has started doing MOHS. I was curious which embedding technique most MOHS techs prefer to use and why? Currently I've been using a heat sink (not attached) to do my embedding. We only have one heat sink, so I'll do my embedding on the free heat sink and then flip that "button" over onto a chuck with semi solid embedding medium. I don't let anything set on top (since we don't have another heat sink). I've done a lot of research on the cryoembedder, but its very expensive and my practice won't go for the cost. I'm interested in trying the slide technique, but wanted to hear from other techs about it. Do you put any embedding medium on the slide first or not? I've seen it described both ways. Any feedback is appreciated, especially on which technique has given the best results for speed and accuracy on full epidermis. Victoria Kline HT (ASCP) Lancaster, PA From michang2014 at gmail.com Sat Feb 25 23:33:06 2017 From: michang2014 at gmail.com (Michelle Chang) Date: Sat, 25 Feb 2017 21:33:06 -0800 Subject: [Histonet] Penetration problem with floating brain sections Message-ID: <782A838F-ACAD-4431-9FAB-5CB7774DAD27@gmail.com> Hi Caroline, For floating sections of 50um, my primary antibody incubation has always been overnight at room temp -- much better staining. You may also want to add Triton-X or Tween 20 to your wash buffer (in case you have not done so). In regards to your slide cracking, where do you observe the crack? If it is peripheral and localized on one side, you may want to check the angle of the blade. If it is throughout, then you may want to check if block has totally sink in sucrose prior to sectioning. Let me know if you have any additional question. Michelle > On Feb 17, 2017, at 10:00 AM, histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Opening in great veterinary diagnostic lab (Johnson, Carole) > 2. penetration problem with floating brain sections (Bass, Caroline) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 16 Feb 2017 20:13:33 +0000 > From: "Johnson, Carole" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Opening in great veterinary diagnostic lab > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > We have a day shift opening in our lab. Beautiful lab, great equipment, awesome pathologists. If you are interested, please follow this link for more information. > > http://www.nmda.nmsu.edu/humanresources/ > > > > Carole L. Johnson, HT(ASCP)cm, QIHC > > New Mexico Department of Agriculture > Veterinary Diagnostic Services > 1101 Camino de Salud, NE > Albuquerque, NM 87101 > 505.383.9299 > > > > > Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. > > > ------------------------------ > > Message: 2 > Date: Fri, 17 Feb 2017 06:56:25 +0000 > From: "Bass, Caroline" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] penetration problem with floating brain sections > Message-ID: > Content-Type: text/plain; charset="utf-8" > > Hello All, > > I?m doing some IHC with DAB on formaldehyde fixed rat brain floating sections, either 35 of 50 um thick. Usually I get fine staining of TH for example, but now I?m going after a neuron that's a bit sparse, and I noticed I have a couple of darkly stained cells. But I can see dozens of ?ghost cells?, these appear to be very lightly stained in the right place. My guess is that the antibody isn?t penetrating fully, and so cells on the face of the sections are staining darkly, and some on the interior are just barely been stained. Any suggestions on improving penetrance? I usually have primary on overnight, shaking in the cold room, and secondary at RT for 2 hours. I was thinking of extending the primary to room temperature and maybe upping the triton-X concentration. Any other suggestions? > > Secondly, I noticed were getting some cracks in the tissue. We usually mount the DAB stained sections on some sort of tissue bond slide, and then let it dry overnight before dilipidating and coverslipping with permeant. My best guess is that sometime during the drying or coverslipping the tissue pulls away slight, resulting in these smallish cracks. > > Anyway, any advice on handling these two issues would be greatly appreciated. > > Thanks, > > Caroline Bass > University at Buffalo > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 159, Issue 15 > ***************************************** From carl.hobbs at kcl.ac.uk Sun Feb 26 11:32:21 2017 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sun, 26 Feb 2017 17:32:21 +0000 Subject: [Histonet] ultraclone RA95101 PGP 9.5 Message-ID: Hi Ultraclone no longer make this Ab, sigh Always a strange Supplier.....Rossiters Farm/Ultraclone's setup always made me think of Royston Vasey However, I have replaced it with Abcam's ab108986 See images of results on ms, rat and human FFPWS on Histonet Images website I think it's excellent ( I use it mainly to see/count C fibres in mouse epithelium so, it's GOT to be good). Abcam also do an excellent Ms monoclonal ( see Histonet image site also) Sure, DAKO's ( Agilent) anti PGP 9.5 is also good for human but, I have not tested it on ms/rat. Or.... Cedarlane: https://www.cedarlanelabs.com/Products/Search?location=1&searchby=Description&supplier=ULTRACL&text=PGP+9.5&x=0&y=0 which offers alternatives. Good luck! Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From aeck at dh.org Mon Feb 27 13:26:54 2017 From: aeck at dh.org (Eck, Allison) Date: Mon, 27 Feb 2017 19:26:54 +0000 Subject: [Histonet] square footage for formalin Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A97341B84@DH-MAIL01.dhorg.org> Good afternoon, We have inspectors here and they are questioning the size of the room in the operating room where they keep their 5 gallon cube. Does anyone know of any square footage requirements for a room that where formalin is kept and used? Thank you in advance Allison Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT) Lead Tech Histology Doylestown Hospital 595 W State St Doylestown, PA 18901 215-345-2264 aeck at dh.org From Timothy.Morken at ucsf.edu Mon Feb 27 14:05:02 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 27 Feb 2017 20:05:02 +0000 Subject: [Histonet] square footage for formalin In-Reply-To: <4ED8C96A8F20FC4F883A92E2A0A0D64A97341B84@DH-MAIL01.dhorg.org> References: <4ED8C96A8F20FC4F883A92E2A0A0D64A97341B84@DH-MAIL01.dhorg.org> Message-ID: <761E2B5697F795489C8710BCC72141FF88E448AB@ex07.net.ucsf.edu> Allison, I've never heard of any such requirement. The only requirements have to do with vapor exposure. If the levels are below the OSHA standards it is ok. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center tim -----Original Message----- From: Eck, Allison via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, February 27, 2017 11:27 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] square footage for formalin Good afternoon, We have inspectors here and they are questioning the size of the room in the operating room where they keep their 5 gallon cube. Does anyone know of any square footage requirements for a room that where formalin is kept and used? Thank you in advance Allison Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT) Lead Tech Histology Doylestown Hospital 595 W State St Doylestown, PA 18901 215-345-2264 aeck at dh.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmcgough at clinlab.com Mon Feb 27 16:19:08 2017 From: jmcgough at clinlab.com (=?utf-8?Q?Jason_McGough?=) Date: Mon, 27 Feb 2017 15:19:08 -0700 Subject: [Histonet] Recycled reagents in VIP processor In-Reply-To: References: Message-ID: Here is our experience with recycled reagents: We have been recycling Xylene and Alcohol since 12-2008. It can work well for you and save you money. It was a challenge?to put it in play where it made the recycling program a good thing. We found out that the theoretical and the reality of recycling are not the same thing and you may need to "find" a way to make it work. The first truth about recycling is that you can recycle and retain a large portion of your alcohol but with special considerations. The recycler that we purchased will only recover a purity quality of about 95-97% alcohol back in product. That meant that we were stock piling huge volumes of 95% and still required?to purchase 100% vendor grade for most processes. This?was a nightmare event for us. I got cleaver and measured the alcohol percentages of our processors (the biggest consumer of alcohols) and found that the actual values in the 100% alcohol (x4) stations were closer to 94%, 96%, 97%, 99% (an alcohol ?hydrometer was used for this measurement). I then realized that I could use the 95% alcohol in the processors to use it up. I modified the preventative maintenance?to change out all of the first 3 alcohols to 95% recycled alcohol and the very last one remains at 100% vendor?grade alcohol, we also do not keep any alcohol that is not at a 95% value or higher. any 70% alcohol is just discarded at change out. (before this recycler plan our preventative maintenance plan was to use the reagents in the processor for 3 weeks by a bump-&-dump method; the first reagent in a series would be dumped each week and all of the reagents of that series after that station?were moved (bumped) down into the station prior, then last station was always new 100% vendor grade reagent. This allowed?us to "use up" the reagents and not replace them to often. This also retains?a certain amount of consistency within the processing?system so microtomy doesn't' experience changes when maintenance?is performed?on the processors.) Along with this?processor modification I adjusted the changing of all these 95% alcohols off ?the instrument twice a week to be sure that they didn't drop too low and affect processing quality, the last station still remains?at 100% vendor grade reagent. I would be glad to give you more details on how this worked for us if you would like to hear more about it or have my help to set up your program. One of my thinking processes for recycling program is to change out the reagents as often as you can/need/ or want to. to keep the quality high enough for the function it is used for. Remember?that you are taking it out of use often but not throwing it away. The conservation?of your reagents, and dollars, lies in the repeated use of the same reagents so ?you do not have to be "stingy" with the preventative maintenance?changes. other thoughts; you still need to purchase 100% vendor grade reagents for some processes, but that is OK, because?that becomes a way to bolster up your recycled quality and fight off the reality of diminishing returns. Recycling?also decreases your waste being hauled off so it decreases the dollars?in waste removal. The recycling of Xylene is excellent?and we get a purity return of about 99.9% back on every recycling run. Once we started the recycling and had a program?in place?that actually?worked for us, and with us, we were operating at a cost of about 30% of the prior operational?cost for our Alcohol and Xylene. We currently are operating at about 36.6% of the reagent costs for Alcohol and?Xylene with todays pricing and our current volumes when compared to the old method of??"dump-&-bump" pm schedules. We have 4 tissue processors and 2 stainers in use regularly. Our workload volumes allow this to work for us and save us a great deal of money. You are welcome to call me and get answers to any questions you may have. Scott Johnson, HTL (ASCP) Histology Supervisor Clinical Laboratory of the Black Hills 605-343-2267 Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgough at clinlab.com www.clinlab.com -----Original message----- > From:Gareth Davis via Histonet > > Sent: Wednesday, February 22, 2017 1:42 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Recycled reagents in VIP processor > > Hi, > I was always told not to use recycled reagents, i.e. Alcohol and Xylene, in > processors. I am using a VIP 300, refurbished, and I would rather not use > recycled reagents in it. But, during the last CAP inspection they > suggested I use the recycled to save money. And now my administration > wants to cut cost. Just wanted to know what labs were doing. > Thanks, > > > -- > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm > Yuma Gastroenterology > Yuma, AZ 85364 > 928-248-5259 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jaylundgren at gmail.com Mon Feb 27 17:25:52 2017 From: jaylundgren at gmail.com (Jay Lundgren) Date: Mon, 27 Feb 2017 17:25:52 -0600 Subject: [Histonet] Recycled reagents in VIP processor In-Reply-To: <34B2EDA118548A4EB35D6E650345BA6469BE016D@SV-EX08.childrensmemorial.org> References: <34B2EDA118548A4EB35D6E650345BA6469BE016D@SV-EX08.childrensmemorial.org> Message-ID: When people say they are saving money by recycling reagents I always wonder if they are including tech time (to run the still) in their calculations. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Feb 22, 2017 at 2:32 PM, Margaryan, Naira via Histonet < histonet at lists.utsouthwestern.edu> wrote: > WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY.... > > Thanks, > Naira > > > Ranked nationally in all 10 pediatric specialties by U.S. News & World > Report (LCHOC Ver 1.0) > > > This message contains confidential information and is intended only for > the individual named. If you are not the named addressee you should not > disseminate, distribute or copy this e-mail. Please notify the sender > immediately by e-mail if you have received this e-mail by mistake and > delete this e-mail from your system. E-mail transmission cannot be > guaranteed to be secure or error-free as information could be intercepted, > corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. > The sender therefore does not accept liability for any errors or omissions > in the contents of this message, which arise as a result of e-mail > transmission. If verification is required please request a hard-copy > version. (LCHOC VER 1.0) > > ________________________________________ > From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] > Sent: Wednesday, February 22, 2017 2:23 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Recycled reagents in VIP processor > > Hi, > I was always told not to use recycled reagents, i.e. Alcohol and Xylene, in > processors. I am using a VIP 300, refurbished, and I would rather not use > recycled reagents in it. But, during the last CAP inspection they > suggested I use the recycled to save money. And now my administration > wants to cut cost. Just wanted to know what labs were doing. > Thanks, > > > -- > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm > Yuma Gastroenterology > Yuma, AZ 85364 > 928-248-5259 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JRobinson at pathology-associates.com Mon Feb 27 17:48:39 2017 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Mon, 27 Feb 2017 23:48:39 +0000 Subject: [Histonet] square footage for formalin In-Reply-To: <4ED8C96A8F20FC4F883A92E2A0A0D64A97341B84@DH-MAIL01.dhorg.org> References: <4ED8C96A8F20FC4F883A92E2A0A0D64A97341B84@DH-MAIL01.dhorg.org> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C98E8539D@PAEXCH1.PathologyAssociates.local> Hi Alison- many years ago I worked in a hospital. We had a very small Frozen Section room in the OR. We did keep a 5 gallon carboy of formalin in there for the convenience of the OR staff so they would have formalin available for their specimens. We were told by someone in "authority" (sorry, can't remember who) that we needed to switch to 1 gallon formalin bottles in the FS room to lessen the chance of a spill. As "luck" would have it, someone on the OR staff opened the last 5 gallon carboy before the switch went into effect and left it on its side. The spigot was barely open and the entire 5 gallons leaked out during the night. They had to call in a HazMat team for cleanup as the fumes were overwhelming. Speaking from experience, I would highly recommend switching over to 1 gallon bottles of formalin for OR use. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Eck, Allison via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, February 27, 2017 11:27 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] square footage for formalin Good afternoon, We have inspectors here and they are questioning the size of the room in the operating room where they keep their 5 gallon cube. Does anyone know of any square footage requirements for a room that where formalin is kept and used? Thank you in advance Allison Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT) Lead Tech Histology Doylestown Hospital 595 W State St Doylestown, PA 18901 215-345-2264 aeck at dh.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From relia1 at earthlink.net Tue Feb 28 11:35:44 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 28 Feb 2017 12:35:44 -0500 Subject: [Histonet] Happy Mardi Gras!!! Exciting Opportunities in CA, CO, TX, TN, AZ, WY, NJ, NY, WI and NC Message-ID: <338401d291e9$190e49e0$4b2adda0$@earthlink.net> Hi Histonetters! Happy Mardi Gras!!!! Are you doing anything special to celebrate Mardi Gras? New Orleans has had a special place in my heart for a long time. My husband proposed to me there many years ago and we try to get there at least once a year. Since we won?t be in NOLA for Mardi Gras this year we found a Mardi Gras block party to go to here in town. So Laissez Les Bon Temps Rouler! That means: Let the good times roll!! I hope you have a chance to add some fun and frivolity to your day!! Instead of asking YOU to ?THROW ME SOMETHING MISTER? Please take a look at my current job openings! All of these are permanent full time openings. I am proud to say that MOST of these are RELIA EXCLUSIVES and all of my clients offer competitive pay, great benefits and great people to work with. Relocation is negotiable. Histology /Lead/ Supervisor/Management: San Diego, CA Santa Ana, CA Flagstaff, AZ Cheyenne, WY Histotechnicians/Histotechnologists: Modesto, CA Wilmington, NC Nashville, TN Kingsport, TN San Diego, CA Anaheim, CA Glenwood Springs, CO Austin, TX Elizabeth, NJ Syracuse, NY Manitowoc, WI If you or anyone you know is interested in hearing more about any of the opportunities or exploring a job search in another area please contact me. You can reach me at relia1 at earthlink.net or toll free at 866-607-3542 or on my cell at 407-353-5070. I will be happy to help, everything is kept in strictest of confidence and if I place someone you refer to me you will earn a referral fee. Thanks-Pam Right Place, Right Time, Right Move with RELIA! March 10, 2017 is Histotechnology Professionals Day. Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From john.frazier at roche.com Tue Feb 28 13:30:46 2017 From: john.frazier at roche.com (Frazier, John) Date: Tue, 28 Feb 2017 14:30:46 -0500 Subject: [Histonet] Recycled reagents in VIP processor Message-ID: <-2110263303007881997@unknownmsgid> As a six sigma consultant to histology laboratories, it has been my experience that recycling xylene and alcohol overall is not a cost saver. When you factor in both capital dollars and operational dollars, the savings is neutral. In addition to the neutral cost in recycling, you have to concern yourself with the quality of your in product on a daily basis. The last piece in the equation is the handling of Xylene with multiple touch points in between. A movement within the histology world has begun with handling xylene (hazard waste) as little as possible and/or reducing or eliminating its use where possible. Sent from my iPhone > On Feb 27, 2017, at 6:25 PM, Jay Lundgren wrote: > > When people say they are saving money by recycling reagents I always wonder > if they are including tech time (to run the still) in their calculations. > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > > On Wed, Feb 22, 2017 at 2:32 PM, Margaryan, Naira via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY.... >> >> Thanks, >> Naira >> >> >> Ranked nationally in all 10 pediatric specialties by U.S. News & World >> Report (LCHOC Ver 1.0) >> >> >> This message contains confidential information and is intended only for >> the individual named. If you are not the named addressee you should not >> disseminate, distribute or copy this e-mail. Please notify the sender >> immediately by e-mail if you have received this e-mail by mistake and >> delete this e-mail from your system. E-mail transmission cannot be >> guaranteed to be secure or error-free as information could be intercepted, >> corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. >> The sender therefore does not accept liability for any errors or omissions >> in the contents of this message, which arise as a result of e-mail >> transmission. If verification is required please request a hard-copy >> version. (LCHOC VER 1.0) >> >> ________________________________________ >> From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] >> Sent: Wednesday, February 22, 2017 2:23 PM >> To: histonet at lists.utsouthwestern.edu >> Subject: [Histonet] Recycled reagents in VIP processor >> >> Hi, >> I was always told not to use recycled reagents, i.e. Alcohol and Xylene, in >> processors. I am using a VIP 300, refurbished, and I would rather not use >> recycled reagents in it. But, during the last CAP inspection they >> suggested I use the recycled to save money. And now my administration >> wants to cut cost. Just wanted to know what labs were doing. >> Thanks, >> >> >> -- >> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm >> Yuma Gastroenterology >> Yuma, AZ 85364 >> 928-248-5259 >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From clemenma at evms.edu Tue Feb 28 13:50:49 2017 From: clemenma at evms.edu (Clements, Mary Ann) Date: Tue, 28 Feb 2017 19:50:49 +0000 Subject: [Histonet] Leica CM1950 Message-ID: <7C3ADBB9F8E8E442B69BDE04519091DB0E845208@Barclay.evms.net> We are interested in purchasing the Leica CM 1950 cryostat. Would love to hear your comments, good, bad or indifferent... Thanks in advance! Mary Ann Clements, B.S. Biorepository Manager Department of Pathology & Anatomy Eastern Virginia Medical School 700 West Olney Road Lewis Hall, Room 3011 Norfolk, VA 23507 email: clemenma at evms.edu phone: (757) 446-7910 fax: (757) 446-7059 From naira.margaryan at hsc.wvu.edu Tue Feb 28 14:39:10 2017 From: naira.margaryan at hsc.wvu.edu (Margaryan, Naira) Date: Tue, 28 Feb 2017 20:39:10 +0000 Subject: [Histonet] Recycled reagents in VIP processor In-Reply-To: <-2110263303007881997@unknownmsgid> References: <-2110263303007881997@unknownmsgid> Message-ID: Absolutely agreed! Naira -----Original Message----- From: Frazier, John [mailto:john.frazier at roche.com] Sent: Tuesday, February 28, 2017 2:31 PM To: Jay Lundgren Cc: Margaryan, Naira ; Gareth Davis ; histonet at lists.utsouthwestern.edu; Margaryan, Naira Subject: Re: [Histonet] Recycled reagents in VIP processor As a six sigma consultant to histology laboratories, it has been my experience that recycling xylene and alcohol overall is not a cost saver. When you factor in both capital dollars and operational dollars, the savings is neutral. In addition to the neutral cost in recycling, you have to concern yourself with the quality of your in product on a daily basis. The last piece in the equation is the handling of Xylene with multiple touch points in between. A movement within the histology world has begun with handling xylene (hazard waste) as little as possible and/or reducing or eliminating its use where possible. Sent from my iPhone > On Feb 27, 2017, at 6:25 PM, Jay Lundgren wrote: > > When people say they are saving money by recycling reagents I always > wonder if they are including tech time (to run the still) in their calculations. > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > > On Wed, Feb 22, 2017 at 2:32 PM, Margaryan, Naira via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY.... >> >> Thanks, >> Naira >> >> >> Ranked nationally in all 10 pediatric specialties by U.S. News & >> World Report (LCHOC Ver 1.0) >> >> >> This message contains confidential information and is intended only >> for the individual named. If you are not the named addressee you >> should not disseminate, distribute or copy this e-mail. Please notify >> the sender immediately by e-mail if you have received this e-mail by >> mistake and delete this e-mail from your system. E-mail transmission >> cannot be guaranteed to be secure or error-free as information could >> be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. >> The sender therefore does not accept liability for any errors or >> omissions in the contents of this message, which arise as a result of >> e-mail transmission. If verification is required please request a >> hard-copy version. (LCHOC VER 1.0) >> >> ________________________________________ >> From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] >> Sent: Wednesday, February 22, 2017 2:23 PM >> To: histonet at lists.utsouthwestern.edu >> Subject: [Histonet] Recycled reagents in VIP processor >> >> Hi, >> I was always told not to use recycled reagents, i.e. Alcohol and >> Xylene, in processors. I am using a VIP 300, refurbished, and I >> would rather not use recycled reagents in it. But, during the last >> CAP inspection they suggested I use the recycled to save money. And >> now my administration wants to cut cost. Just wanted to know what labs were doing. >> Thanks, >> >> >> -- >> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology >> Yuma, AZ 85364 >> 928-248-5259 >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From garethdavisyuma at gmail.com Tue Feb 28 14:57:24 2017 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Tue, 28 Feb 2017 13:57:24 -0700 Subject: [Histonet] Recycled reagents in VIP processor In-Reply-To: References: <-2110263303007881997@unknownmsgid> Message-ID: When using Xylene in a lab, it's my opinion that the savings is associated with the size of the lab. I am in a very small lab, where I am the only Histotech. I recycle both alcohol and Xylene and monitor both closely. For me, it would cost effective to recycle and use as much as possible, instead of purchasing fresh. But, I agree, if it's a larger lab it may not be as cost effective. Also, if the lab is in the position to order a substitute for Xylene, where it would be cheaper, than that seems to be the optimum thing to do. On Tue, Feb 28, 2017 at 1:39 PM, Margaryan, Naira < naira.margaryan at hsc.wvu.edu> wrote: > Absolutely agreed! > > Naira > > -----Original Message----- > From: Frazier, John [mailto:john.frazier at roche.com] > Sent: Tuesday, February 28, 2017 2:31 PM > To: Jay Lundgren > Cc: Margaryan, Naira ; Gareth Davis < > garethdavisyuma at gmail.com>; histonet at lists.utsouthwestern.edu; Margaryan, > Naira > Subject: Re: [Histonet] Recycled reagents in VIP processor > > As a six sigma consultant to histology laboratories, it has been my > experience that recycling xylene and alcohol overall is not a cost saver. > When you factor in both capital dollars and operational dollars, the > savings is neutral. In addition to the neutral cost in recycling, you have > to concern yourself with the quality of your in product on a daily basis. > The last piece in the equation is the handling of Xylene with multiple > touch points in between. A movement within the histology world has begun > with handling xylene (hazard > waste) as little as possible and/or reducing or eliminating its use where > possible. > > Sent from my iPhone > > > On Feb 27, 2017, at 6:25 PM, Jay Lundgren wrote: > > > > When people say they are saving money by recycling reagents I always > > wonder if they are including tech time (to run the still) in their > calculations. > > > > Sincerely, > > > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > On Wed, Feb 22, 2017 at 2:32 PM, Margaryan, Naira via Histonet < > > histonet at lists.utsouthwestern.edu> wrote: > > > >> WOULD LIKE TO KNOW AS WELL - IT IS WASTE OF MONEY.... > >> > >> Thanks, > >> Naira > >> > >> > >> Ranked nationally in all 10 pediatric specialties by U.S. News & > >> World Report (LCHOC Ver 1.0) > >> > >> > >> This message contains confidential information and is intended only > >> for the individual named. If you are not the named addressee you > >> should not disseminate, distribute or copy this e-mail. Please notify > >> the sender immediately by e-mail if you have received this e-mail by > >> mistake and delete this e-mail from your system. E-mail transmission > >> cannot be guaranteed to be secure or error-free as information could > >> be intercepted, corrupted, lost, destroyed, arrive late or incomplete, > or contain viruses. > >> The sender therefore does not accept liability for any errors or > >> omissions in the contents of this message, which arise as a result of > >> e-mail transmission. If verification is required please request a > >> hard-copy version. (LCHOC VER 1.0) > >> > >> ________________________________________ > >> From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] > >> Sent: Wednesday, February 22, 2017 2:23 PM > >> To: histonet at lists.utsouthwestern.edu > >> Subject: [Histonet] Recycled reagents in VIP processor > >> > >> Hi, > >> I was always told not to use recycled reagents, i.e. Alcohol and > >> Xylene, in processors. I am using a VIP 300, refurbished, and I > >> would rather not use recycled reagents in it. But, during the last > >> CAP inspection they suggested I use the recycled to save money. And > >> now my administration wants to cut cost. Just wanted to know what labs > were doing. > >> Thanks, > >> > >> > >> -- > >> Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology > >> Yuma, AZ 85364 > >> 928-248-5259 > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From jclark at pcnm.com Tue Feb 28 15:37:10 2017 From: jclark at pcnm.com (Joanne Clark) Date: Tue, 28 Feb 2017 21:37:10 +0000 Subject: [Histonet] Histonet Digest, Vol 159, Issue 24 In-Reply-To: References: Message-ID: <16783308458c430e8849092c4733aee2@E15MADAG-D06N04.sh11.lan> Hi Allison, I talked to my safety guy and there isn't anything specific. They are telling you that because there is a fire hazard warning on formalin cubes, but we know that the change of it catching fire is slim to nil. They want you to use the same standards as with alcohol and xylene (1 gallon per 100 square feet). It is all so dependent on who you have inspecting you and what their background is. Joanne Clark, HT Pathology Consultants of New Mexico ---------------------------------------------------------------------- Message: 1 Date: Mon, 27 Feb 2017 19:26:54 +0000 From: "Eck, Allison" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] square footage for formalin Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A97341B84 at DH-MAIL01.dhorg.org> Content-Type: text/plain; charset="us-ascii" Good afternoon, We have inspectors here and they are questioning the size of the room in the operating room where they keep their 5 gallon cube. Does anyone know of any square footage requirements for a room that where formalin is kept and used? Thank you in advance Allison Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT) Lead Tech Histology Doylestown Hospital 595 W State St Doylestown, PA 18901 215-345-2264 aeck at dh.org ------------------------------ Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains.