From jqb7 at cdc.gov Mon Dec 4 11:32:35 2017 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Mon, 4 Dec 2017 17:32:35 +0000 Subject: [Histonet] Contract position at CDC Atlanta Message-ID: All, We have an opening for a Histotech in the Infectious Diseases Pathology Branch at the Centers for Disease Control here in Atlanta. It is a contractor position and I have been told it has been listed at the IHRC site. If you are interested, please apply. Thanks! Jeanine H. Sanders Infectious Diseases Pathology Branch Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS-G32 Atlanta, GA 30329 From abadesuyi at nrh-ok.com Mon Dec 4 13:14:53 2017 From: abadesuyi at nrh-ok.com (Adesuyi, Banjo) Date: Mon, 4 Dec 2017 19:14:53 +0000 Subject: [Histonet] ANP.10255 Message-ID: <4ea201e15dce48ef808fec40982ea340@nrh-ok.com> Good afternoon, Please I am wondering whether you can direct me to where I can get a policy for the pathologist's participation in a peer educational program and internal audits Best regards, Adesupo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS, Supervisor, Histology Section abadesuyi at nrh-ok.com O: 405-307-1145 M: 405-973-6363 F: 405-307-1143 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From amosbrooks at gmail.com Mon Dec 4 19:08:59 2017 From: amosbrooks at gmail.com (Amos Brooks) Date: Mon, 4 Dec 2017 20:08:59 -0500 Subject: [Histonet] Science Haiku Message-ID: Anyone that heard Science Friday recently may have heard of this. I thought it would be fun to see what others come up with. So many people think I'm an archaeologist when I tell them I'm a Histotech. This makes for a fun elevator pitch for our profession. Scientists are challenged to summarize their work in a haiku. Here's mine... Sick organs need tests It's microscopic study Thats histology #flamechallenge http://www.stonybrook.edu/happenings/community-outreach/35567/ From lmarie08 at uga.edu Tue Dec 5 07:32:39 2017 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Tue, 5 Dec 2017 13:32:39 +0000 Subject: [Histonet] looking for diff cell counter Message-ID: Good Morning Histonet colleagues, My PI wants me to purchase a differential cell counter and I was wondering if anyone had any recommendations for a basic manual counter. One that offers the ability to count 5 different cell types and dings at every 100 cells. I found one through Fisher but it seems pricey. Does anyone know other places to get them? Thanks! Lauren From mburns at atlanticurologyclinics.com Tue Dec 5 07:45:40 2017 From: mburns at atlanticurologyclinics.com (Melissa Burns) Date: Tue, 5 Dec 2017 13:45:40 +0000 Subject: [Histonet] Noise Levels in Lab Message-ID: <8C537C608EA0194B87F6DE7BC6E1BF885BF11CBF@AUC-Exchange2.gsuro.com> Does anyone monitor the noise levels in their lab? If so, what is your protocol? Do you have a device to do this? Thanks in advance :) Melissa From aj.taylor at blueyonder.co.uk Tue Dec 5 10:03:07 2017 From: aj.taylor at blueyonder.co.uk (Alan) Date: Tue, 5 Dec 2017 16:03:07 +0000 Subject: [Histonet] Microtomy of Equine Tendon Blocks Message-ID: Greetings to all on Histonet. I have a particularly challenging question regarding the sectioning of Equine tendon material. My colleague and I recently received a number of TS tendon slices. Unfortunately they had been placed in 90% IDA for fixation approximately 10 years ago, where they remained up to the point they were sent to our lab for cutting and staining. The blocks are extremely hard, almost like undecalcified bone. They have processed well into paraffin, but we have wrecked several disposable blades just in facing the blocks. They have a very gritty feel on trimming even at 2 microns. We have tried some commercial softening agents, including Mollifex, with long immersion over days, rather than the usual hour or so, we have tried neat fabric softener and 10% phenol in 70% IDA. We have also left some blocks in molten paraffin for several days, as this sometimes works with very fibrous tissues. As does prolonged chilling with ice and water. We have managed to recover a small number of reasonable sections, at present we are looking at 1 block = 1 blade to obtain a very few working sections. Do any histotechs out there have any secret recipes that they are prepared to share and have made up themselves, or a popular in-house reagent that they know is reliable, that they have enjoyed success with in their labs, or references to softening solutions for very difficult tissues, that may be historical or more recent. We have, of course, asked the referring lab to place all future freshly dissected tissues immediately into 10%NBF. Thank you for any assistance you are able to offer. Yours sincerely Alan Taylor BSc(Hons). FRMS Microtechnical Services Exeter. UK www.microtechnicalservices.co.uk Sent from Mail for Windows 10 From Timothy.Morken at ucsf.edu Tue Dec 5 10:01:59 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 5 Dec 2017 16:01:59 +0000 Subject: [Histonet] Noise Levels in Lab In-Reply-To: <8C537C608EA0194B87F6DE7BC6E1BF885BF11CBF@AUC-Exchange2.gsuro.com> References: <8C537C608EA0194B87F6DE7BC6E1BF885BF11CBF@AUC-Exchange2.gsuro.com> Message-ID: We don't but there are decibel monitor phone apps. Not sure how accurate they are... I've used one called Sound Meter. It shows 70+ db next to our -80 freezer and 90+ db next to the 6-foot fume hood. In our lab with cryostats it shows 60+ and in a quiet lab, no equipment running, it shows 50+ Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Melissa Burns via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, December 05, 2017 5:46 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Noise Levels in Lab Does anyone monitor the noise levels in their lab? If so, what is your protocol? Do you have a device to do this? Thanks in advance :) Melissa _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vicki.kalscheur at wisc.edu Tue Dec 5 12:41:07 2017 From: vicki.kalscheur at wisc.edu (Vicki Kalscheur) Date: Tue, 5 Dec 2017 18:41:07 +0000 Subject: [Histonet] Histonet Digest, Vol 169, Issue 3 In-Reply-To: References: Message-ID: Tendons - use a tungsten carbide blade/ 50 degree. -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Tuesday, December 5, 2017 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 169, Issue 3 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. ANP.10255 (Adesuyi, Banjo) 2. Science Haiku (Amos Brooks) 3. looking for diff cell counter (Lauren Sweeney) 4. Noise Levels in Lab (Melissa Burns) 5. Microtomy of Equine Tendon Blocks (Alan) 6. Re: Noise Levels in Lab (Morken, Timothy) ---------------------------------------------------------------------- Message: 1 Date: Mon, 4 Dec 2017 19:14:53 +0000 From: "Adesuyi, Banjo" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] ANP.10255 Message-ID: <4ea201e15dce48ef808fec40982ea340 at nrh-ok.com> Content-Type: text/plain; charset="us-ascii" Good afternoon, Please I am wondering whether you can direct me to where I can get a policy for the pathologist's participation in a peer educational program and internal audits Best regards, Adesupo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS, Supervisor, Histology Section abadesuyi at nrh-ok.com O: 405-307-1145 M: 405-973-6363 F: 405-307-1143 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ Message: 2 Date: Mon, 4 Dec 2017 20:08:59 -0500 From: Amos Brooks To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Science Haiku Message-ID: Content-Type: text/plain; charset="UTF-8" Anyone that heard Science Friday recently may have heard of this. I thought it would be fun to see what others come up with. So many people think I'm an archaeologist when I tell them I'm a Histotech. This makes for a fun elevator pitch for our profession. Scientists are challenged to summarize their work in a haiku. Here's mine... Sick organs need tests It's microscopic study Thats histology #flamechallenge http://www.stonybrook.edu/happenings/community-outreach/35567/ ------------------------------ Message: 3 Date: Tue, 5 Dec 2017 13:32:39 +0000 From: Lauren Sweeney To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] looking for diff cell counter Message-ID: Content-Type: text/plain; charset="us-ascii" Good Morning Histonet colleagues, My PI wants me to purchase a differential cell counter and I was wondering if anyone had any recommendations for a basic manual counter. One that offers the ability to count 5 different cell types and dings at every 100 cells. I found one through Fisher but it seems pricey. Does anyone know other places to get them? Thanks! Lauren ------------------------------ Message: 4 Date: Tue, 5 Dec 2017 13:45:40 +0000 From: Melissa Burns To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Noise Levels in Lab Message-ID: <8C537C608EA0194B87F6DE7BC6E1BF885BF11CBF at AUC-Exchange2.gsuro.com> Content-Type: text/plain; charset="us-ascii" Does anyone monitor the noise levels in their lab? If so, what is your protocol? Do you have a device to do this? Thanks in advance :) Melissa ------------------------------ Message: 5 Date: Tue, 5 Dec 2017 16:03:07 +0000 From: Alan To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Microtomy of Equine Tendon Blocks Message-ID: Content-Type: text/plain; charset="utf-8" Greetings to all on Histonet. I have a particularly challenging question regarding the sectioning of Equine tendon material. My colleague and I recently received a number of TS tendon slices. Unfortunately they had been placed in 90% IDA for fixation approximately 10 years ago, where they remained up to the point they were sent to our lab for cutting and staining. The blocks are extremely hard, almost like undecalcified bone. They have processed well into paraffin, but we have wrecked several disposable blades just in facing the blocks. They have a very gritty feel on trimming even at 2 microns. We have tried some commercial softening agents, including Mollifex, with long immersion over days, rather than the usual hour or so, we have tried neat fabric softener and 10% phenol in 70% IDA. We have also left some blocks in molten paraffin for several days, as this sometimes works with very fibrous tissues. As does prolonged chilling with ice and water. We have managed to recover a small number of reasonable sections, at present we are looking at 1 block = 1 blade to obtain a very few working sections. Do any histotechs out there have any secret recipes that they are prepared to share and have made up themselves, or a popular in-house reagent that they know is reliable, that they have enjoyed success with in their labs, or references to softening solutions for very difficult tissues, that may be historical or more recent. We have, of course, asked the referring lab to place all future freshly dissected tissues immediately into 10%NBF. Thank you for any assistance you are able to offer. Yours sincerely Alan Taylor BSc(Hons). FRMS Microtechnical Services Exeter. UK www.microtechnicalservices.co.uk Sent from Mail for Windows 10 ------------------------------ Message: 6 Date: Tue, 5 Dec 2017 16:01:59 +0000 From: "Morken, Timothy" To: Melissa Burns Cc: Histonet Subject: Re: [Histonet] Noise Levels in Lab Message-ID: Content-Type: text/plain; charset="us-ascii" We don't but there are decibel monitor phone apps. Not sure how accurate they are... I've used one called Sound Meter. It shows 70+ db next to our -80 freezer and 90+ db next to the 6-foot fume hood. In our lab with cryostats it shows 60+ and in a quiet lab, no equipment running, it shows 50+ Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Melissa Burns via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, December 05, 2017 5:46 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Noise Levels in Lab Does anyone monitor the noise levels in their lab? If so, what is your protocol? Do you have a device to do this? Thanks in advance :) Melissa _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 169, Issue 3 **************************************** From tbraud at holyredeemer.com Tue Dec 5 13:17:01 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 5 Dec 2017 19:17:01 +0000 Subject: [Histonet] Sound monitoring Message-ID: <48E053DDF6CE074DB6A7414BA05403F84CEA136B@HRHEX03-HOS.holyredeemer.local> We contract with an outside company to come in and perform a sound monitor once a year in each room of the lab Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal ***************************** From Karen.Heckford at DignityHealth.org Wed Dec 6 12:11:09 2017 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Wed, 6 Dec 2017 18:11:09 +0000 Subject: [Histonet] Quick release clamp Message-ID: <5dddf20ec4cf4c60a58909638107a513@PHX-EXCH-013.chw.edu> Good Morning, Does anyone know where to get the Quick release clamp repair kits for the microtome? The one that has the springs in it. I remember a long time ago getting them but I cannot remember from where. Do they still sell them? Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you From jobposting2600 at gmail.com Wed Dec 6 15:03:29 2017 From: jobposting2600 at gmail.com (Office Manager) Date: Wed, 6 Dec 2017 14:03:29 -0700 Subject: [Histonet] Please post Message-ID: Look for Primera Signature Slide Printer or similar, used and regardless of condition. Please contact: Isaac Malagon imalagon at evgastro.com (480) 231-9464 From G.Spoelstra at murdoch.edu.au Thu Dec 7 07:04:52 2017 From: G.Spoelstra at murdoch.edu.au (Gerard Spoelstra) Date: Thu, 7 Dec 2017 13:04:52 +0000 Subject: [Histonet] Flatbed microwave oven for microwave staining antigen retrieval Message-ID: Hi We currently use a 25 year old turntable kitchen microwave oven. This is used for antigen retrieval, silver staining and several other histochemical stains. When I out shopping the other day a salesman introduced me to the flatbed microwave that heats from below and reputedly gives more even heating at a constant rate . Is anyone using such an oven in the laboratory. If so what is your experience of it? thanks Gerard Spoelstra Veterinary Histology Murdoch University From sandra.cheasty at wisc.edu Thu Dec 7 12:22:58 2017 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Thu, 7 Dec 2017 18:22:58 +0000 Subject: [Histonet] Lab Vision 720-2D Parts Message-ID: Hello all, I am in need of some parts for our Lab Vision 720-2D IHC stainer that don't cost an arm and a leg. (Plunger, plastic piece that holds the wide top of the plunger, tubing, stopcock, connections.) If you e-mail me, I can send a picture of exactly what I'm looking for. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From JMacDonald at mtsac.edu Thu Dec 7 19:17:52 2017 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Thu, 7 Dec 2017 17:17:52 -0800 Subject: [Histonet] CSH call for abstracts Message-ID: Hi All, The California Society for Histotechnology will have its annual symposium in Anaheim, CA May 4-6, 2018. If you are interested in presenting please send me your abstract. Thank you, Jennifer From G.Spoelstra at murdoch.edu.au Thu Dec 7 20:25:18 2017 From: G.Spoelstra at murdoch.edu.au (Gerard Spoelstra) Date: Fri, 8 Dec 2017 02:25:18 +0000 Subject: [Histonet] Microtomy of Equine Tendon Blocks In-Reply-To: References: Message-ID: Hi Alan We have had success with horse's hoof using potassium hydroxide prior to processing. After the hoof is fixed for several weeks we leave the hoof for 12 hours sometimes longer in 10% Potassium Hydroxide and then further trim the tissue in. The Potassium Hydroxide has a marked affect in softening the tissue without affecting the staining. The tissue is processed as usual. We all our process the tissue with our paraffin temperature set at 60 degrees C. This gives better infiltration of hard tissue. I would try with one block. Take it back to water leave it in NBF for O/N the leave it in 10% Potassium Hydroxide for 12 hours. After washing in water process as usual. regards Gerard Spoelstra Veterinary Histology Murdoch University Perth Western Australia ________________________________ From: Alan via Histonet Sent: Wednesday, 6 December 2017 12:03:07 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Microtomy of Equine Tendon Blocks Greetings to all on Histonet. I have a particularly challenging question regarding the sectioning of Equine tendon material. My colleague and I recently received a number of TS tendon slices. Unfortunately they had been placed in 90% IDA for fixation approximately 10 years ago, where they remained up to the point they were sent to our lab for cutting and staining. The blocks are extremely hard, almost like undecalcified bone. They have processed well into paraffin, but we have wrecked several disposable blades just in facing the blocks. They have a very gritty feel on trimming even at 2 microns. We have tried some commercial softening agents, including Mollifex, with long immersion over days, rather than the usual hour or so, we have tried neat fabric softener and 10% phenol in 70% IDA. We have also left some blocks in molten paraffin for several days, as this sometimes works with very fibrous tissues. As does prolonged chilling with ice and water. We have managed to recover a small number of reasonable sections, at present we are looking at 1 block = 1 blade to obtain a very few working sections. Do any histotechs out there have any secret recipes that they are prepared to share and have made up themselves, or a popular in-house reagent that they know is reliable, that they have enjoyed success with in their labs, or references to softening solutions for very difficult tissues, that may be historical or more recent. We have, of course, asked the referring lab to place all future freshly dissected tissues immediately into 10%NBF. Thank you for any assistance you are able to offer. Yours sincerely Alan Taylor BSc(Hons). FRMS Microtechnical Services Exeter. UK www.microtechnicalservices.co.uk [http://www.microtechnicalservices.co.uk/images/microtech%20services3.jpg] Microtechnical Services - for technical excellence in ... www.microtechnicalservices.co.uk Microtechnical Services based in Exeter, Devon provide a comprehensive range of technical histology services, a comprehensive listing of tissue sections, both stained ... Sent from Mail for Windows 10 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet From Erin.Martin at ucsf.edu Mon Dec 11 04:37:55 2017 From: Erin.Martin at ucsf.edu (Martin, Erin) Date: Mon, 11 Dec 2017 10:37:55 +0000 Subject: [Histonet] ROS1 Message-ID: Good morning histoland! Has anyone had success with the ROS1 antibody from CellSignaling? I just can't seem to get it working beyond what our pathologist call "a faint blush". I would greatly appreciate if you would be wiling to share your protocol! Have a lovely Monday! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. From Richard.Cartun at hhchealth.org Mon Dec 11 09:06:56 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 11 Dec 2017 15:06:56 +0000 Subject: [Histonet] ROS1 In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E9549CBCB@HHCEXCHMB03.hhcsystem.org> Yes, we've had excellent results using their clone "D4D6". We use it at 1:50 dilution on the Leica Bond Max following high-pH retrieval. Please contact me directly if you require additional information. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Martin, Erin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, December 11, 2017 5:38 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] ROS1 This email is from outside HHC. BE CAREFUL when opening attachments or links from unknown senders. Good morning histoland! Has anyone had success with the ROS1 antibody from CellSignaling? I just can't seem to get it working beyond what our pathologist call "a faint blush". I would greatly appreciate if you would be wiling to share your protocol! Have a lovely Monday! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From tabbott at pennstatehealth.psu.edu Mon Dec 11 15:45:30 2017 From: tabbott at pennstatehealth.psu.edu (Abbott, Tanya) Date: Mon, 11 Dec 2017 21:45:30 +0000 Subject: [Histonet] Primera cassette engraver Message-ID: <6e563ed2f4514d359e4236d868bbeda0@EXCHSRVR12.mshmc.local> Has anyone out there set up a Primera cassette engraver with CoPath? If so, could you reach out to me please, I have some questions!! Any help is greatly appreciated!!! Best, Tanya Tanya G. Abbott Pathology Manager PennState Health St. Joseph Reading Pennsylvania tabbott at pennstatehealth.psu.edu 610-378-2635 From liliane.meunier at gmail.com Tue Dec 12 09:33:14 2017 From: liliane.meunier at gmail.com (Liliane Meunier) Date: Tue, 12 Dec 2017 10:33:14 -0500 Subject: [Histonet] Problem with breast cancer TMA - core are mooving Message-ID: Hi I recently did a H&E coloration on a TMA that was cut the day before. TMA contain 300 breast tumor core. After the coloration, I noticed that a lot of core had moove. I put slide 20 minutes in an oven at 60oC prior to coloration Thanks a lot for your suggestions Liliane Meunier Assistante de recherche Laboratoire Dre Anne-Marie Mes-Masson Institut du Cancer de Montr?al Plateforme Pathologie Mol?culaire CRCHUM 900, rue St-Denis 10e ?tage Montr?al, Qc H2X 0A9 514-890-8000 poste 31296 From Shannon.Logan at bellin.org Tue Dec 12 10:22:29 2017 From: Shannon.Logan at bellin.org (Logan, Shannon) Date: Tue, 12 Dec 2017 16:22:29 +0000 Subject: [Histonet] Problem with breast cancer TMA - core are mooving In-Reply-To: References: Message-ID: <8636f4f3a5c044c3af9eecb8a67456ae@BAPWEXCH001b.bellin.com> Hello Liliane, I would suggest longer time in 60 degree oven before H&E staining? perhaps 60 min. Also, if you are not using ?plus? or coated slides , I would try using them. Good luck, Shannon H. Logan B.S., HTL (ASCP) Pathology Department Bellin Health Memorial Hospital 744 South Webster Avenue Green Bay, WI 54305-3400 920-433-3653 X3727 From: Liliane Meunier via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, December 12, 2017 9:33 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Problem with breast cancer TMA - core are mooving Hi I recently did a H&E coloration on a TMA that was cut the day before. TMA contain 300 breast tumor core. After the coloration, I noticed that a lot of core had moove. I put slide 20 minutes in an oven at 60oC prior to coloration Thanks a lot for your suggestions Liliane Meunier Assistante de recherche Laboratoire Dre Anne-Marie Mes-Masson Institut du Cancer de Montr?al Plateforme Pathologie Mol?culaire CRCHUM 900, rue St-Denis 10e ?tage Montr?al, Qc H2X 0A9 514-890-8000 poste 31296 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Tue Dec 12 10:23:15 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 12 Dec 2017 11:23:15 -0500 Subject: [Histonet] Happy Holidays!!! Message-ID: <09da01d37365$83b53b60$8b1fb220$@earthlink.net> Happy Holidays Histonetters!! I hope you are enjoying a wonderful holiday season!! Cookies Baked? Presents wrapped? Christmas cards mailed? Job Hunting is probably the LAST thing on your mind BUT. You never know when that perfect opportunity might be waiting for you. Is your next career move under the Christmas tree here at RELIA? Do you know someone who is looking ? A new position could be the best gift EVER!! If you have a minute I would like to tell you about the jobs that I am working on. All my clients are willing to move now or wait until after the holidays to accommodate you. The labs and hospitals that I work with offer excellent compensation packages including great pay and benefits and relocation assistance. RELIA SPOTLIGHT OPPORTUNITIES!! . Product Manager - IHC/Molecular -San Francisco, CA . Histology Supervisor - Dermpath - Birmingham, AL . Histology Technician - Private Lab - Austin, TX . Lead Histotech - Dermpath - Birmingham, AL . Grossing Histotech - GI Lab - San Diego, CA If you or anyone you know might be interested in hearing about any of these opportunities or are looking for a new position in another area please feel free to contact me or pass along my contact information to them. I would love to send you a referral fee! J I can be reached toll free at 866-307-3542, on my cell/text at 407-353-5070 and via email at relia1 at earthlink.net Thanks and again. Happy Holidays to You and Yours! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From angie at ka-recruiting.com Tue Dec 12 13:10:23 2017 From: angie at ka-recruiting.com (Angie Laparidis) Date: Tue, 12 Dec 2017 14:10:23 -0500 Subject: [Histonet] Bench histotech, Supervisor, & Director roles Message-ID: Hello Histonetters! How is your job search coming along? Are you looking for a new histology role to start off your new year with a fresh start!? I wanted to reach out to share some active permanent, Full-time Histology openings available nationwide. Would you would like more details? Please send over an updated resume and let me know the best way to get a hold of you! FL - Miami- Histotech (1:00am - 9:30am) GA - Atlanta - Histotech (M-F, 6am-2:30pm) IN - Outside of Chicago- Histotech (midnight & evening shifts) MN - Minneapolis - Histotech (day shift, M-F 8a-5p) NM - Southern- Assistant Director of Histology NY - Buffalo - Histotech (day float schedule) NY - New York City- Histotech NV - Reno - Histotech (M-F 5am ? 1:30pm) OR - Salem - Histology Supervisor VA - Richmond - Histotechnologist (day shift) Look forward to hearing from you and Happy Holidays! Sincerely, *Angie Laparidis*Healthcare Recruiter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South, Boston, MA, 02109 W: 617.746-2744 F: (617) 507-8009 *Angie at ka-recruiting.com* Our openings are updated daily at www.ka-recruiting.com From tkngflght at yahoo.com Tue Dec 12 13:24:27 2017 From: tkngflght at yahoo.com (Cheryl) Date: Tue, 12 Dec 2017 19:24:27 +0000 (UTC) Subject: [Histonet] Houston area PRN grosser - weekday afternoons References: <660348859.3905054.1513106668090.ref@mail.yahoo.com> Message-ID: <660348859.3905054.1513106668090@mail.yahoo.com> Hi 'Netters -? We're looking for a grossing tech for our South Houston laboratory.? ?Must be CLIA eligible (complex specimens) and strong preference for someone with experience.? ?The work is mostly biopsies and template-driven. Great crew, beautiful clean facilities-- it's a good extra income situation.? Could grow into part time or remain PRN-- open to whatever works.? Send inquiries with resume to info at adgpath.com?Cheryl Kerry, HT(ASCP)?ADG Houston Pathology From LRaff at uropartners.com Wed Dec 13 08:39:24 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 13 Dec 2017 14:39:24 +0000 Subject: [Histonet] A blog about pathology and histology Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF11777E35@COLOEXCH01.uropartners.local> Can you make the diagnosis? Click the link to give it a try. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From Blanca.Lopez at UTSouthwestern.edu Wed Dec 13 10:25:24 2017 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Wed, 13 Dec 2017 16:25:24 +0000 Subject: [Histonet] TMA cutting advice Message-ID: Morning Histonettes, I am looking for more options to get nice sections on my TMA during cutting...I make this breast TMA, we used old blocks from other places everything was fine during the construction until I have to cut it. IT's brittle some cores and others curl as soon they touch the knife. I soaked it in ammonium/H2O, I cooler longer, I even used lotion to make it soft and more tricks that I know but any worked. I am getting frustrating with this block ( I did not have this problem with my others TMA's). Is anybody knows more tricks that help me to get nice sections? I am willing to try everything. Thanks for your help. Blanca Lopez HT (ASCP) ________________________________ UT Southwestern Medical Center The future of medicine, today. From sandra.cheasty at wisc.edu Wed Dec 13 13:37:43 2017 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Wed, 13 Dec 2017 19:37:43 +0000 Subject: [Histonet] Vacuum Breaker Error on Prisma H&E Stainer Message-ID: Hello all, We have been getting an occasional, (maybe once every 20 runs), error 90 on our Prisma stainer. (Vacuum Breaker Error) Has anyone encountered this? Does it have to do with the incoming water lines? I'm trying to avoid a costly visit by a Sakura vetted repair tech. Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From relia1 at earthlink.net Wed Dec 13 14:09:21 2017 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 13 Dec 2017 15:09:21 -0500 Subject: [Histonet] Here's a Real GEM from a few years back. From Ashley Troutman - The Twelve Days of Christmas for Histotechs! Message-ID: <00cc01d3744e$43aa9440$caffbcc0$@earthlink.net> Hi Histonetters! Here is a gem found on the histonet in 12/2013 from Ashley Troutman The 12 days of Christmas for Histotechs Enjoy Histopeeps: Hello all, I hope the holidays are treating everyone well. (Especially in light of us having the most hazardous job in the country...) Here's some fun for the season: The Twelve Days of Histo TWELVE Medical Directors ELEVEN logs-a-printing TEN residents reading NINE frozen sections EIGHT administrators SEVEN stainers beeping SIX techs complaining FIVE I...H...Cs.... :) FOUR calling docs THREE recuts TWO special stains And a block with an H and EEEEE... Please feel free to sing along. Merry Christmas in Histoland! Ashley Troutman BS, MBA, HT(ASCP) QIHC Thanks-Pam Happy Holidays !!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From aj.taylor at blueyonder.co.uk Thu Dec 14 09:15:56 2017 From: aj.taylor at blueyonder.co.uk (Alan) Date: Thu, 14 Dec 2017 15:15:56 +0000 Subject: [Histonet] Horse Tendon Microtomy Message-ID: Dear All Thank you to everyone who replied to my recent request for help with the above. As these were particularly tough tendons we finally used a combination of KOH and a disposable tungsten blade which gave us fairly good sections, more suitable for scanning than our previous efforts. Thanks to you all for your assistance. Best wishes Alan Taylor BSc(Hons). FRMS. Microtechnical Services Exeter. UK. www.microtechnicalservices.co.uk Sent from Mail for Windows 10 From VKurth at uwhealth.org Thu Dec 14 09:18:20 2017 From: VKurth at uwhealth.org (Kurth Virginia L.) Date: Thu, 14 Dec 2017 15:18:20 +0000 Subject: [Histonet] Stat Specimens Message-ID: What types of specimens do other institutions deem as appropriate for STAT processing? Currently our institution allows biopsies for rejection, graft Vs host, r/o BK or CMV. Thanks for any input! Jenny University Hospital of Wisconsin From criley at dpspa.com Thu Dec 14 11:01:37 2017 From: criley at dpspa.com (Charles Riley) Date: Thu, 14 Dec 2017 12:01:37 -0500 Subject: [Histonet] Slide disposal Message-ID: Does anyone know a cheap legal way to dispose of slides? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From mward at wakehealth.edu Fri Dec 15 11:06:10 2017 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Fri, 15 Dec 2017 17:06:10 +0000 Subject: [Histonet] Prostate needle biopsies Message-ID: I am posting this question for our Histology manager. For prostate needle biopsies how many levels and unstained slides are people cutting and also how long after the case is signed out is everyone keeping the unstained slides that have been cut? Thanks in advance for everyone's help. Martha Ward Wake Forest Baptist Health Winston-Salem, NC 25157 From tbraud at holyredeemer.com Fri Dec 15 14:51:00 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 15 Dec 2017 20:51:00 +0000 Subject: [Histonet] Prostate Needle Biopsies Message-ID: <48E053DDF6CE074DB6A7414BA05403F84CEA2F6D@HRHEX03-HOS.holyredeemer.local> We cut levels x2 with no unstained. We've never had a problem going back to a block for additional recuts or specials. We date all our precut IHC slides (patient or control) with an expiration date of 6 weeks. Slides cut for histochemistry stains seem to have no expiration. Hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Prostate needle biopsies (Martha Ward-Pathology) ---------------------------------------------------------------------- Message: 1 Date: Fri, 15 Dec 2017 17:06:10 +0000 From: Martha Ward-Pathology Subject: [Histonet] Prostate needle biopsies I am posting this question for our Histology manager. For prostate needle biopsies how many levels and unstained slides are people cutting and also how long after the case is signed out is everyone keeping the unstained slides that have been cut? Thanks in advance for everyone's help. Martha Ward Wake Forest Baptist Health Winston-Salem, NC 25157 From rsrichmond at gmail.com Fri Dec 15 18:18:27 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 15 Dec 2017 19:18:27 -0500 Subject: [Histonet] Prostate needle biopsies Message-ID: Martha Ward at Wake Forest Baptist Health, Winston-Salem, North Carolina asks: >>I am posting this question for our Histology manager. For prostate needle biopsies how many levels and unstained slides are people cutting and also how long after the case is signed out is everyone keeping the unstained slides that have been cut?<< A reasonable protocol is 5 sections from each block, cut subserially onto slides suitable for IHC. Stain slides 1, 3, and 5 with H&E for immediate review by the pathologist. Roughly 10% of cases will require immunohistochemistry on slides 2 and 4. Many histotechnologists refuse to do this, but recutting loses about half the features of interest, compared to IHC done on pre-cut slides 2 and 4. I don't know about retaining slides 2 and 4 in the 90% of cases where no IHC is necessary. Prolonged retention isn't necessary, though. Robert S. Richmond Samurai Pathologist Maryville TN From Douglas.Porter at sparrow.org Fri Dec 15 18:39:20 2017 From: Douglas.Porter at sparrow.org (Porter, Douglas) Date: Sat, 16 Dec 2017 00:39:20 +0000 Subject: [Histonet] Prostate needle biopsies In-Reply-To: References: Message-ID: <22cec8bad14e43b9966db4d9b8b62359@EXCHMBPVAS02.shs.org> We have stopped cutting unstained slides as physicians have started asking for the block to be sent for molecular studies. When we cut unstained slides, there isn't enough of the specimen left for those studies. Douglas A. Porter, HT (ASCP) Pathologist Assistant Anatomic Pathology IT Coordinator Sparrow Laboratories Department of Pathology 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) douglas.porter at sparrow.org -----Original Message----- From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, December 15, 2017 7:18 PM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Prostate needle biopsies Martha Ward at Wake Forest Baptist Health, Winston-Salem, North Carolina asks: >>I am posting this question for our Histology manager. For prostate >>needle biopsies how many levels and unstained slides are people cutting and also how long after the case is signed out is everyone keeping the unstained slides that have been cut?<< A reasonable protocol is 5 sections from each block, cut subserially onto slides suitable for IHC. Stain slides 1, 3, and 5 with H&E for immediate review by the pathologist. Roughly 10% of cases will require immunohistochemistry on slides 2 and 4. Many histotechnologists refuse to do this, but recutting loses about half the features of interest, compared to IHC done on pre-cut slides 2 and 4. I don't know about retaining slides 2 and 4 in the 90% of cases where no IHC is necessary. Prolonged retention isn't necessary, though. Robert S. Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=qgJBLQvENW4Kb9JcrSOXvjOKPeZu-lgWMRRjbRk-vYI&r=FB_j03RJqXZhrS71GzU-qcyfg0DRiu5KOssLLNE66qE&m=9fORAJ_DzaATk6xonLS8oPu-PupYgimucCF8H-7HLwI&s=Uq5kZq99sIAFZGFsazk8bTQ9LsAg5dyKIyw0uuabcc8&e= ________________________________ CONFIDENTIALITY NOTICE: This email communication may contain private, confidential, or legally privileged information intended for the sole use of the designated and/or duly authorized recipient(s). If you are not the intended recipient or have received this email in error, please notify the sender immediately by email and permanently delete all copies of this email including all attachments without reading them. If you are the intended recipient, secure the contents in a manner that conforms to all applicable state and/or federal requirements related to privacy and confidentiality of such information. ________________________________ From jqb7 at cdc.gov Tue Dec 19 08:52:20 2017 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue, 19 Dec 2017 14:52:20 +0000 Subject: [Histonet] PCR sectioning Message-ID: Good morning! Do any of you out there section for PCR? If so, do you have a preference in microtomes? Thanks! Jeanine H. Sanders Infectious Diseases Pathology Branch Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS-G32 Atlanta, GA 30329 From hmarlatt26 at gmail.com Tue Dec 19 10:12:24 2017 From: hmarlatt26 at gmail.com (Heather Marlatt) Date: Tue, 19 Dec 2017 16:12:24 +0000 Subject: [Histonet] Mouse bladder Message-ID: Does anyone have a good protocol for mouse bladder embedded in agar that they are willing to share? We have mouse bladders in 2% agar and have tried a few different protocol variations from our usual mouse but the agar keeps shriveling up. Thanks Heather From liz at premierlab.com Tue Dec 19 11:29:33 2017 From: liz at premierlab.com (Liz Chlipala) Date: Tue, 19 Dec 2017 17:29:33 +0000 Subject: [Histonet] Mouse bladder In-Reply-To: References: Message-ID: I would try histogel instead of agar. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Heather Marlatt via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, December 19, 2017 9:12 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Mouse bladder Does anyone have a good protocol for mouse bladder embedded in agar that they are willing to share? We have mouse bladders in 2% agar and have tried a few different protocol variations from our usual mouse but the agar keeps shriveling up. Thanks Heather _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From cforster at umn.edu Tue Dec 19 11:44:39 2017 From: cforster at umn.edu (Colleen Forster) Date: Tue, 19 Dec 2017 11:44:39 -0600 Subject: [Histonet] Mouse bladder In-Reply-To: References: Message-ID: Be sure you are processing them on an overnight process. Even if the samples are small it takes that time to process the agar properly. I use Histogel and learned this lesson the hard way. My samples always "shriveled" up too until someone enlightened me about the processing time. I would think agar might be the same. Just a thought, Colleen Forster HT(ASCP)QIHC U of MN On Tue, Dec 19, 2017 at 11:29 AM, Liz Chlipala via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I would try histogel instead of agar. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz at premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > From: Heather Marlatt via Histonet [mailto:histonet at lists. > utsouthwestern.edu] > Sent: Tuesday, December 19, 2017 9:12 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Mouse bladder > > Does anyone have a good protocol for mouse bladder embedded in agar that > they are willing to share? We have mouse bladders in 2% agar and have tried > a few different protocol variations from our usual mouse but the agar keeps > shriveling up. > > Thanks > Heather > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From faith14913 at aol.com Tue Dec 19 13:34:49 2017 From: faith14913 at aol.com (faith14913 at aol.com) Date: Tue, 19 Dec 2017 14:34:49 -0500 Subject: [Histonet] tissue blocks Message-ID: <16070457a91-1720-18103@webjas-vac170.srv.aolmail.net> Does anyone have any breast tissue or skin FFPE blocks that would be willing to send me? 5 blocks of each would be greatly appreciated. From dunatrsd at sbcglobal.net Tue Dec 19 15:09:04 2017 From: dunatrsd at sbcglobal.net (dusko trajkovic) Date: Tue, 19 Dec 2017 21:09:04 +0000 (UTC) Subject: [Histonet] Mouse bladder In-Reply-To: References: Message-ID: <534776587.1396592.1513717744605@mail.yahoo.com> I agree with Colleen, processing is the issue. You need to process on a longer program in order to dehydrate and clear the agar properly.dusko On Tuesday, December 19, 2017 9:45 AM, Colleen Forster via Histonet wrote: Be sure you are processing them on an overnight process. Even if the samples are small it takes that time to process the agar properly. I use Histogel and learned this lesson the hard way. My samples always "shriveled" up too until someone enlightened me about the processing time. I would think agar might be the same. Just a thought, Colleen Forster HT(ASCP)QIHC U of MN On Tue, Dec 19, 2017 at 11:29 AM, Liz Chlipala via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I would try histogel instead of agar. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz at premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > From: Heather Marlatt via Histonet [mailto:histonet at lists. > utsouthwestern.edu] > Sent: Tuesday, December 19, 2017 9:12 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Mouse bladder > > Does anyone have a good protocol for mouse bladder embedded in agar that > they are willing to share? We have mouse bladders in 2% agar and have tried > a few different protocol variations from our usual mouse but the agar keeps > shriveling up. > > Thanks > Heather > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster at umn.edu Tue Dec 19 15:50:07 2017 From: cforster at umn.edu (Colleen Forster) Date: Tue, 19 Dec 2017 15:50:07 -0600 Subject: [Histonet] Mouse bladder In-Reply-To: <534776587.1396592.1513717744605@mail.yahoo.com> References: <534776587.1396592.1513717744605@mail.yahoo.com> Message-ID: Exactly...and then it wont shrivel up.... C On Tue, Dec 19, 2017 at 3:09 PM, dusko trajkovic wrote: > I agree with Colleen, processing is the issue. You need to process on a > longer program in order to dehydrate and clear the agar properly. > dusko > > > On Tuesday, December 19, 2017 9:45 AM, Colleen Forster via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Be sure you are processing them on an overnight process. Even if the > samples are small it takes that time to process the agar properly. > > I use Histogel and learned this lesson the hard way. My samples always > "shriveled" up too until someone enlightened me about the processing time. > I would think agar might be the same. > > > Just a thought, > > Colleen Forster HT(ASCP)QIHC > U of MN > > On Tue, Dec 19, 2017 at 11:29 AM, Liz Chlipala via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > I would try histogel instead of agar. > > > > Liz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > > Premier Laboratory, LLC > > PO Box 18592 > > Boulder, CO 80308 > > (303) 682-3949 office > > (303) 682-9060 fax > > (303) 881-0763 cell > > liz at premierlab.com > > www.premierlab.com > > > > Ship to Address: > > > > Premier Laboratory, LLC > > 1567 Skyway Drive, Unit E > > > Longmont, CO 80504 > > > > > From: Heather Marlatt via Histonet [mailto:histonet at lists. > > utsouthwestern.edu] > > Sent: Tuesday, December 19, 2017 9:12 AM > > To: histonet at lists.utsouthwestern.edu > > Subject: [Histonet] Mouse bladder > > > > Does anyone have a good protocol for mouse bladder embedded in agar that > > they are willing to share? We have mouse bladders in 2% agar and have > tried > > a few different protocol variations from our usual mouse but the agar > keeps > > shriveling up. > > > > Thanks > > Heather > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu utsouthwestern.edu > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ________________________________ > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From sandra.cheasty at wisc.edu Wed Dec 20 12:43:54 2017 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Wed, 20 Dec 2017 18:43:54 +0000 Subject: [Histonet] Hard Water and Auto Stainers Message-ID: Hello all, Thanks for everyone's response to error 90 on our Prisma stainer; it has been cleared! Does anyone use filtered, softened, or DI water in their H&E stainer to avoid clogging up the system? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From mward at wakehealth.edu Wed Dec 20 12:59:38 2017 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Wed, 20 Dec 2017 18:59:38 +0000 Subject: [Histonet] Recycling formalin Message-ID: I am posting this for our Histology lab manager: Is anyone recycling formalin and if so, exactly what are you using the recycled formalin for? Putting it on processors, adding to specimens, etc? Apparently our EHS department is pushing this. Also thank everyone for their help with our questions about unstained slides for prostate biopsies. It was very helpful. Thanks in advance for your help with our recycled formalin question. Martha Ward Wake Forest Baptist Health 336-716-2109 From Timothy.Morken at ucsf.edu Wed Dec 20 13:15:56 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 20 Dec 2017 19:15:56 +0000 Subject: [Histonet] FW: Recycling formalin References: Message-ID: Martha, The question to ask your EHS is how much extra exposure they will allow employees. Recycling removes all the salts so you need to add them back. Then it is just like making up formalin from scratch. Does anyone do that anymore? You need a minimum of full-face vapor masks to prevent vapor exposure. And risk of spill is high. Our EH&S decided it was not worth the risk. Filtering is another option, but, again, you are handling large amounts of formalin so risk high exposure and possible spills. We tried it and it was too labor intensive, the filter was constantly clogging and we had to keep large carboys around that seem to just leak due to the poor quality of the spigots. Again, we abandoned it. The other questions are, who is going to do this (s EHS volunteering their staff?!?!)? Is there space to do it (waste carboys, plus filled carboys, plus transporting)? I can bet your lab does not have space or personnel for this procedure. Does EHS? Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Martha Ward-Pathology via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, December 20, 2017 11:00 AM To: histonet at lists.utsouthwestern.edu Cc: histonet at lists2.utsouthwestern.edu Subject: [Histonet] Recycling formalin I am posting this for our Histology lab manager: Is anyone recycling formalin and if so, exactly what are you using the recycled formalin for? Putting it on processors, adding to specimens, etc? Apparently our EHS department is pushing this. Also thank everyone for their help with our questions about unstained slides for prostate biopsies. It was very helpful. Thanks in advance for your help with our recycled formalin question. Martha Ward Wake Forest Baptist Health 336-716-2109 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Wed Dec 20 13:18:30 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 20 Dec 2017 19:18:30 +0000 Subject: [Histonet] Hard Water and Auto Stainers In-Reply-To: References: Message-ID: Sandra, yes, we use DI water from a water system in our lab Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Sandra Cheasty via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, December 20, 2017 10:44 AM To: Histonet (histonet at lists.utsouthwestern.edu) Subject: [Histonet] Hard Water and Auto Stainers Hello all, Thanks for everyone's response to error 90 on our Prisma stainer; it has been cleared! Does anyone use filtered, softened, or DI water in their H&E stainer to avoid clogging up the system? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Thu Dec 21 11:45:00 2017 From: patpxs at gmail.com (P Sicurello) Date: Thu, 21 Dec 2017 09:45:00 -0800 Subject: [Histonet] Hard water and Prisma stainer Message-ID: Good Morning Listers, I am very interested in this topic but I can't find the thread for this topic, does someone have it? What is error 90 on a Prisma and what does it mean? Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From melissa at alliedsearchpartners.com Thu Dec 21 13:25:37 2017 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Thu, 21 Dec 2017 19:25:37 +0000 Subject: [Histonet] IHC Supervisor Job in CA Message-ID: Hello, I am handling a search for an IHC Supervisor position for permanent employment in Los Angeles, CA. This is a Day Shift opportunity. Must be strong in IHC and have supervisory experience. Contact me today if you are interested in this! Thank you and Happy Holidays! Melissa Owens, CHP President, Laboratory Staffing Allied Search Partners Direct Line: 407.413.9117 Toll Free: 888.388.7571 ext. 102 F: 888.388.7572 From patpxs at gmail.com Fri Dec 22 11:19:03 2017 From: patpxs at gmail.com (P Sicurello) Date: Fri, 22 Dec 2017 09:19:03 -0800 Subject: [Histonet] Embedding Mold Cleaning Device Message-ID: Good Morning Listers, There used to be on the market a small gizmo that cleaned embedding molds. Does anyone have one and can tell me where or who to buy it from? I know we can use the processors to clean them, but for our circumstances it would be more convenient to have one of those table-top mold cleaning machines. Thank you and welcome to winter. (where here in San Diego the temperatures are in the upper 60's) - oh wait! that's because all the fires are heating us up ;-) Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From oneilb at wvumedicine.org Fri Dec 22 12:29:33 2017 From: oneilb at wvumedicine.org (O'neil, Beth) Date: Fri, 22 Dec 2017 18:29:33 +0000 Subject: [Histonet] Histonet Digest, Vol 169, Issue 16 In-Reply-To: References: Message-ID: <3CEB8EBCF9C7A648B9694B5696462A71730D962B@NT-EX1.wvuhs.com> We have a HistosMate from Milestone Medical. It is wonderful. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor, Technical Specialist Lab: 304 - 293 - 6014 Office: 304 - 293 - 7629 oneilb at wvumedicine.org ------------------------------ Message: 2 Date: Fri, 22 Dec 2017 09:19:03 -0800 From: P Sicurello To: HistoNet Subject: [Histonet] Embedding Mold Cleaning Device Message-ID: Content-Type: text/plain; charset="UTF-8" Good Morning Listers, There used to be on the market a small gizmo that cleaned embedding molds. Does anyone have one and can tell me where or who to buy it from? I know we can use the processors to clean them, but for our circumstances it would be more convenient to have one of those table-top mold cleaning machines. Thank you and welcome to winter. (where here in San Diego the temperatures are in the upper 60's) - oh wait! that's because all the fires are heating us up ;-) Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> ________________________________ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rmacapagal at sidra.org Wed Dec 27 01:40:36 2017 From: rmacapagal at sidra.org (Roald Vincent Macapagal) Date: Wed, 27 Dec 2017 07:40:36 +0000 Subject: [Histonet] Rapid H&E Message-ID: <9D4E1FFDC9676A448B6FFF2357C9E093273D7F71@MV3WEXMX04PRV.smrc.sidra.org> Hi all, I would like to ask if anyone can share with me a rapid H&E staining method and procedure for Leica ST4020 linear stainer using the infinity kit from Leica. We are targeting for a 2 minute staining procedure. Thanks! Best regards, Roald Vincent Macapagal, RMT, ASCPi(MLS(CM)) Technologist I - Histopathology and Morgue - Department of Pathology Sidra Medicine rmacapagal at sidra.org Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. From rmacapagal at sidra.org Wed Dec 27 01:51:15 2017 From: rmacapagal at sidra.org (Roald Vincent Macapagal) Date: Wed, 27 Dec 2017 07:51:15 +0000 Subject: [Histonet] Rapid frozen section H&E Message-ID: <9D4E1FFDC9676A448B6FFF2357C9E093273D7F83@MV3WEXMX04PRV.smrc.sidra.org> Hi all, I would like to ask if anyone can share with me a rapid frozen section H&E staining method and procedure for Leica ST4020 linear stainer using the infinity kit from Leica. We are targeting for a 2 minute staining procedure. Thanks! Best regards, Roald Vincent Macapagal, RMT, ASCPi(MLS(CM)) Technologist I - Histopathology and Morgue - Department of Pathology Sidra Medicine rmacapagal at sidra.org Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. From michaelpathology at live.com Wed Dec 27 06:33:08 2017 From: michaelpathology at live.com (Michael Backhus) Date: Wed, 27 Dec 2017 12:33:08 +0000 Subject: [Histonet] Histology Book Message-ID: [https://images-na.ssl-images-amazon.com/images/I/51q5mEF1q1L._AA210_.jpg] Histology Hacks CreateSpace Independent Publishing Platform $11.99 $9.45 Hi, I just published my first histology book called Histology Hacks. I?m very excited and wanted to share it with everyone. It is a new, unique histology book that teaches over 75 ?hacks? or ?histo-hacks ? to improve a histotechnician?s quality of work (i.e slide sections). Such ?hacks? use dryer sheets, soap, aluminum foil and much more. I categorized these hacks into Four Divisions: Embedding, Microtomy, Frozen Sections and Grossing. I have demonstrated each "hack" and determined its effectiveness. I explain the science behind each technique. I think you will find this book intriguing and useful. Let me know what you think. Thank you Mike Backhus B.A HT(ASCP) Sent using Amazon Mobile for iPhone From relia1 at earthlink.net Wed Dec 27 10:20:43 2017 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 27 Dec 2017 11:20:43 -0500 Subject: [Histonet] "There's no place like home for the holidays" Message-ID: <000001d37f2e$a5632e00$f0298a00$@earthlink.net> Hello Histonetters, I hope you are enjoying a wonderful holiday season and are looking forward to an exciting 2018! Like the subject line in this email and the popular holiday carol says. "There's no place like home for the holidays" Can I get you HOME for NEXT year? I sent a similar note to my clients pointing out that there may be some people looking to be home permanently by next Christmas and am in discussion with clients all over the U.S. about positions. If you are someone who might consider a job change with or without relocating in 2018 please drop me a quick email with your desired location. My phone has been ringing off the hook!! I need to know: . Where you are! . What you want to do! . Where you want to be! If you are looking immediately or interested in ANY of the following areas call my cell at 407-353-5070!! Or drop me a quick email at relia1 at earthlink.net I have current openings in the following areas: . Austin, TX . Charlotte, NC . Falmouth, MA . San Diego, CA . Toledo, OH . Concord, CA . Louisville, KY . Birmingham, AL Have a great day and a safe and Happy New Year!!! Happy Holidays !!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Richard.Cartun at hhchealth.org Wed Dec 27 15:20:08 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Wed, 27 Dec 2017 21:20:08 +0000 Subject: [Histonet] Beta-Amyloid antibody for IHC Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E954A6E7D@HHCEXCHMB03.hhcsystem.org> We are looking for a good antibody to detect Beta-Amyloid in formalin-fixed, paraffin-embedded human brain tissue. Any recommendations? We have been using the clone "6E10" which we purchased from Signet Laboratories back in 2001. Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From cforster at umn.edu Wed Dec 27 15:23:00 2017 From: cforster at umn.edu (Colleen Forster) Date: Wed, 27 Dec 2017 15:23:00 -0600 Subject: [Histonet] Beta-Amyloid antibody for IHC In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E954A6E7D@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E954A6E7D@HHCEXCHMB03.hhcsystem.org> Message-ID: Do you knot want to use 6E10 anymore? I believe you can still get it from Biolegends? https://www.biolegend.com/en-us/products/purified-anti-beta-amyloid--1-16-antibody-11228 Respectfully, Colleen Forster HT(ASCP)QIHC *University of Minnesota* On Wed, Dec 27, 2017 at 3:20 PM, Cartun, Richard via Histonet < histonet at lists.utsouthwestern.edu> wrote: > We are looking for a good antibody to detect Beta-Amyloid in > formalin-fixed, paraffin-embedded human brain tissue. Any > recommendations? We have been using the clone "6E10" which we purchased > from Signet Laboratories back in 2001. Thank you! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & > Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Richard.Cartun at hhchealth.org Wed Dec 27 15:34:29 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Wed, 27 Dec 2017 21:34:29 +0000 Subject: [Histonet] Beta-Amyloid antibody for IHC In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E954A6E7D@HHCEXCHMB03.hhcsystem.org> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E954A6E93@HHCEXCHMB03.hhcsystem.org> No, I would like to use ?6E10? if it?s still available. Thank you! Richard From: Colleen Forster [mailto:cforster at umn.edu] Sent: Wednesday, December 27, 2017 4:23 PM To: Cartun, Richard Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Beta-Amyloid antibody for IHC CAUTION: This email is from outside HHC. USE CARE when opening attachments or links. Do you knot want to use 6E10 anymore? I believe you can still get it from Biolegends? https://www.biolegend.com/en-us/products/purified-anti-beta-amyloid--1-16-antibody-11228 Respectfully, Colleen Forster HT(ASCP)QIHC University of Minnesota On Wed, Dec 27, 2017 at 3:20 PM, Cartun, Richard via Histonet > wrote: We are looking for a good antibody to detect Beta-Amyloid in formalin-fixed, paraffin-embedded human brain tissue. Any recommendations? We have been using the clone "6E10" which we purchased from Signet Laboratories back in 2001. Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. From cforster at umn.edu Wed Dec 27 15:40:08 2017 From: cforster at umn.edu (Colleen Forster) Date: Wed, 27 Dec 2017 15:40:08 -0600 Subject: [Histonet] Beta-Amyloid antibody for IHC In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E954A6E93@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E954A6E7D@HHCEXCHMB03.hhcsystem.org> <9215BD4B0BA1B44D962A71C758B68D2E954A6E93@HHCEXCHMB03.hhcsystem.org> Message-ID: You are welcome....it should be the original one by SIgnet...then COvance....now BioLEgends,,,, C On Wed, Dec 27, 2017 at 3:34 PM, Cartun, Richard < Richard.Cartun at hhchealth.org> wrote: > No, I would like to use ?6E10? if it?s still available. Thank you! > > > > *Richard* > > > > *From:* Colleen Forster [mailto:cforster at umn.edu] > *Sent:* Wednesday, December 27, 2017 4:23 PM > *To:* Cartun, Richard > *Cc:* histonet at lists.utsouthwestern.edu > *Subject:* Re: [Histonet] Beta-Amyloid antibody for IHC > > > > CAUTION: This email is from outside HHC. USE CARE when opening attachments > or links. > > Do you knot want to use 6E10 anymore? I believe you can still get it from > Biolegends? > > https://www.biolegend.com/en-us/products/purified-anti- > beta-amyloid--1-16-antibody-11228 > > Respectfully, > > Colleen Forster HT(ASCP)QIHC > > *University of Minnesota* > > > > On Wed, Dec 27, 2017 at 3:20 PM, Cartun, Richard via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > We are looking for a good antibody to detect Beta-Amyloid in > formalin-fixed, paraffin-embedded human brain tissue. Any > recommendations? We have been using the clone "6E10" which we purchased > from Signet Laboratories back in 2001. Thank you! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & > Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > > Hartford, CT 06102 > > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > *Reminder: This e-mail and any attachments are subject to the current HHC > email retention policies. Please save or store appropriately in accordance > with policy. * > From W.E.J.Hoekert at olvg.nl Thu Dec 28 01:57:20 2017 From: W.E.J.Hoekert at olvg.nl (Hoekert, Willem) Date: Thu, 28 Dec 2017 07:57:20 +0000 Subject: [Histonet] [EXTERN] Re: Beta-Amyloid antibody for IHC In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E954A6E7D@HHCEXCHMB03.hhcsystem.org> <9215BD4B0BA1B44D962A71C758B68D2E954A6E93@HHCEXCHMB03.hhcsystem.org>, Message-ID: If not available anymore: Dako clone 6F/3D. Pretreatment with formic acid, works very good. Willem ________________________________________ Van: Colleen Forster via Histonet [histonet at lists.utsouthwestern.edu] Verzonden: woensdag 27 december 2017 22:40 Aan: Cartun, Richard CC: histonet at lists.utsouthwestern.edu Onderwerp: [EXTERN] Re: [Histonet] Beta-Amyloid antibody for IHC You are welcome....it should be the original one by SIgnet...then COvance....now BioLEgends,,,, C On Wed, Dec 27, 2017 at 3:34 PM, Cartun, Richard < Richard.Cartun at hhchealth.org> wrote: > No, I would like to use ?6E10? if it?s still available. Thank you! > > > > *Richard* > > > > *From:* Colleen Forster [mailto:cforster at umn.edu] > *Sent:* Wednesday, December 27, 2017 4:23 PM > *To:* Cartun, Richard > *Cc:* histonet at lists.utsouthwestern.edu > *Subject:* Re: [Histonet] Beta-Amyloid antibody for IHC > > > > CAUTION: This email is from outside HHC. USE CARE when opening attachments > or links. > > Do you knot want to use 6E10 anymore? I believe you can still get it from > Biolegends? > > https://www.biolegend.com/en-us/products/purified-anti- > beta-amyloid--1-16-antibody-11228 > > Respectfully, > > Colleen Forster HT(ASCP)QIHC > > *University of Minnesota* > > > > On Wed, Dec 27, 2017 at 3:20 PM, Cartun, Richard via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > We are looking for a good antibody to detect Beta-Amyloid in > formalin-fixed, paraffin-embedded human brain tissue. Any > recommendations? We have been using the clone "6E10" which we purchased > from Signet Laboratories back in 2001. Thank you! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & > Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > > Hartford, CT 06102 > > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > *Reminder: This e-mail and any attachments are subject to the current HHC > email retention policies. Please save or store appropriately in accordance > with policy. * > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met elektronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. De algemene Inkoopvoorwaarden van de Vereniging Santeon respectievelijk de leden van de Vereniging Santeon zijn van toepassing op en maken integraal onderdeel uit van alle rechtsbetrekkingen, daaronder mede verstaan alle aanvragen, aanbiedingen en overeenkomsten waarbij OLVG optreedt als koper c.q. verwerver van goederen of diensten. Deze voorwaarden zijn op 13 september 2010 gedeponeerd bij de Kamer van Koophandel Midden Nederland en te raadplegen via (http://www.olvg.nl/algemene_inkoopvoorwaarden) www.olvg.nl/algemene_inkoopvoorwaarden From carl.hobbs at kcl.ac.uk Thu Dec 28 13:06:43 2017 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Thu, 28 Dec 2017 19:06:43 +0000 Subject: [Histonet] Beta-Amyloid antibody for IHC Message-ID: If you use clone 6E10 it will demonstrate beta Amyloid only, after Formic acid AR After Citric acid HIER, APP will also be demonstrated Imho Check out Biosource ( Invitrogen) 44-344 and 44-348? Something about x-reactivity between Abeta 1-42 and Abeta 1-43? Or not..... I'd be interested to know if this is valid Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From nipuna.we at gmail.com Thu Dec 28 13:30:10 2017 From: nipuna.we at gmail.com (Nipuna Weerasinghe) Date: Thu, 28 Dec 2017 12:30:10 -0700 Subject: [Histonet] Modified GMS Protocols Message-ID: I would like to know from subject matter expert why KMno4 never used as an alternative oxidant to CrO3 in Modified GMS. Oxidation of 1,2-glycol linkages in carbohydrates to aldehyde groups can be done by KMNO4 and used to do the same in Castella?s potassium permanganate-Schiff reaction, and Gordon and Sweets' reticulum. Moreover, KMNO4 is a strong oxidant that can oxidized aldehyde to carboxylic, so this leads to closer mimicking of function of CrO3. Also why people only tried periodic and not any other oxidant to replace CrO3. I could not find any primary literates concnering this matter. Only handful of attempts with periodic is there. Thanks for your answers in advanced Lip. From gu.lang at gmx.at Sat Dec 30 02:44:33 2017 From: gu.lang at gmx.at (Gudrun Lang) Date: Sat, 30 Dec 2017 09:44:33 +0100 Subject: [Histonet] Modified GMS Protocols In-Reply-To: References: Message-ID: <000c01d3814a$6b894e80$429beb80$@gmx.at> Biological stains were mainly found by trial and error through the century. I think the main goals were to find a good result (for the purpose) within the available resources (cheap / expensive, easy/difficult to get) and with a practical application. Later also health-concerns played a role, that eliminated some protocols. I believe, that the "forefathers" had a hard time to find the best-working protocols. And after that, the histological community acts upon the sentence "never change a winning team" and sticks to the protocols. And we have to admit, that the chemical knowledge of the histologists and the histological knowledge of the chemists may be rather decreased than grown bigger. (a lack of universal scientists) For the diverse oxidants I think (without literature as evidence) it is also a practical matter. The oxidizising result depends on strength of acid and duration of incubation. If you use a very strong oxidant, the time has to be watched very carefully and there is the risk of overoxidation. If you use a weaker oxidant, you have a longer time-space for a sufficient result. (5 min in periodic acid may refer to 20 sek in potassiumpermanganat ?) Gudrun -----Urspr?ngliche Nachricht----- Von: Nipuna Weerasinghe via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Donnerstag, 28. Dezember 2017 20:30 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Modified GMS Protocols I would like to know from subject matter expert why KMno4 never used as an alternative oxidant to CrO3 in Modified GMS. Oxidation of 1,2-glycol linkages in carbohydrates to aldehyde groups can be done by KMNO4 and used to do the same in Castella?s potassium permanganate-Schiff reaction, and Gordon and Sweets' reticulum. Moreover, KMNO4 is a strong oxidant that can oxidized aldehyde to carboxylic, so this leads to closer mimicking of function of CrO3. Also why people only tried periodic and not any other oxidant to replace CrO3. I could not find any primary literates concnering this matter. Only handful of attempts with periodic is there. Thanks for your answers in advanced Lip. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nipuna.we at gmail.com Sun Dec 31 06:56:23 2017 From: nipuna.we at gmail.com (Nipuna Weerasinghe) Date: Sun, 31 Dec 2017 05:56:23 -0700 Subject: [Histonet] Modified GMS Protocols In-Reply-To: <000c01d3814a$6b894e80$429beb80$@gmx.at> References: <000c01d3814a$6b894e80$429beb80$@gmx.at> Message-ID: Thanks for your reply Gudrun, I agree with your statements. If KMnO4 used as a replacement for CrO3, it should be used under much milder conditions (low temperature and less incubation time) than H6IO5 as KMnO4 is a stronger oxidant. However, my question was due to the fact that KMnO4 was a commonly used oxidant and often compared with both CrO3 and H6IO5. Lillie and McManus showed that all three can be used to stain same carbohydrate targets (Modified PAS).[1][2] Even some of the recent papers discuss about GMS based on KMnO4 oxidation but I cannot see protocols, on-market kits or papers actually reporting any.[3] If KMnO4 can work in PAS why it is not tried in GMS? So I thought histologists work in the filed have some experience. [1]Lillie, R. D. Histochemical Comparison of the Casella, Bauer, and Periodic Acid Oxidation-Schiff Leucofuchsin Technics. *Stain. Technol.* 1951, *26*, 123?136. [2]McManus, J. F. A. Periodate Oxidation Techniques. In General Cytochemical Methods; Academy Press Inc., 1961; Vol. 2 [3]Speranza, V. D.; Fail, R. A Common Mistake When Staining for Fungi. Histol. Tech. Bull. Histotechnol. 2004. On Sat, Dec 30, 2017 at 1:44 AM, Gudrun Lang wrote: > Biological stains were mainly found by trial and error through the > century. I think the main goals were to find a good result (for the > purpose) within the available resources (cheap / expensive, easy/difficult > to get) and with a practical application. > Later also health-concerns played a role, that eliminated some protocols. > I believe, that the "forefathers" had a hard time to find the best-working > protocols. And after that, the histological community acts upon the > sentence "never change a winning team" and sticks to the protocols. And we > have to admit, that the chemical knowledge of the histologists and the > histological knowledge of the chemists may be rather decreased than grown > bigger. (a lack of universal scientists) > > For the diverse oxidants I think (without literature as evidence) it is > also a practical matter. The oxidizising result depends on strength of acid > and duration of incubation. If you use a very strong oxidant, the time has > to be watched very carefully and there is the risk of overoxidation. If you > use a weaker oxidant, you have a longer time-space for a sufficient result. > (5 min in periodic acid may refer to 20 sek in potassiumpermanganat ?) > > Gudrun > > > > -----Urspr?ngliche Nachricht----- > Von: Nipuna Weerasinghe via Histonet [mailto:histonet at lists. > utsouthwestern.edu] > Gesendet: Donnerstag, 28. Dezember 2017 20:30 > An: histonet at lists.utsouthwestern.edu > Betreff: [Histonet] Modified GMS Protocols > > I would like to know from subject matter expert why KMno4 never used as an > alternative oxidant to CrO3 in Modified GMS. Oxidation of 1,2-glycol > linkages in carbohydrates to aldehyde groups can be done by KMNO4 and used > to do the same in Castella?s potassium permanganate-Schiff reaction, and > Gordon and Sweets' reticulum. Moreover, KMNO4 is a strong oxidant that can > oxidized aldehyde to carboxylic, so this leads to closer mimicking of > function of CrO3. > > Also why people only tried periodic and not any other oxidant to replace > CrO3. I could not find any primary literates concnering this matter. Only > handful of attempts with periodic is there. > > Thanks for your answers in advanced > > Lip. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rsrichmond at gmail.com Sun Dec 31 14:34:25 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Sun, 31 Dec 2017 15:34:25 -0500 Subject: [Histonet] Modified GMS Protocols Message-ID: If you choose another oxidizing agent than CrO3 (such as KMnO4, potassium permanganate) it's up to you either to find literature to back you up, or to do the stain with appropriate controls for the fungus you're looking for. Freida Carson (citation below) found that periodic acid, commonly substituted for chromic acid in the GMS stain, would stain Histoplasma capsulatum only with the periodic acid at 60 degrees C for an hour. In North America (and I note that Gudrun Lang is in Austria) staining of old Histoplasma capsulatum is the definitive test of the efficacy of the stain. Since this organism doesn't occur in Europe, I don't know know what fungus is the most challenging there. Inconsistent Detection of Histoplasma capsulatum with Periodic Acid Oxidation in the Grocott Methenamine-Silver Nitrate (GMS) Fungus Stain Freida L. Carson, Jerry Fredenburgh, and John E. Maxwell 1. Department of Pathology, Baylor University Medical Center 2. Richard-Allan Scientific Kalamazoo MI 3. Glenwood Regional Medical Center, West Monroe LA J Histotechnol [June] 1999;22:119 Bob Richmond Samurai Pathologist Maryville TN