From relia1 at earthlink.net Tue Aug 1 11:54:33 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 1 Aug 2017 12:54:33 -0400 Subject: [Histonet] Can you help? Message-ID: <05e501d30ae6$d9b32af0$8d1980d0$@earthlink.net> Hi Histonetters, How are you? I hope you are having a great week! I was hoping you might be able to help me. I am presently working with several clients throughout the state of California in need of histotechs and histology supervisors. These are full time permanent positions. My clients offer competitive pay, relocation assistance and great teams to work with. They are ready to interview and hire NOW! Here Are The Opportunities: Histology Supervisor - Modesto, CA Histology Supervisor - Irvine, CA Lead Histotech - Los Angeles, CA Grossing Histotech - San Diego Histotech - Los Angeles The help I need from you is do you know anyone that might be interested in hearing about either of these opportunities? If so could you please forward my e-mail to them? If you are interested in this position please contact me ASAP toll free at 866-607-3542, cell/text 407-353-5070 or via email at relia1 at earthlink.net If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won't release it to anyone without your permission. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From victor_tobias at comcast.net Tue Aug 1 16:14:52 2017 From: victor_tobias at comcast.net (victor_tobias at comcast.net) Date: Tue, 1 Aug 2017 14:14:52 -0700 Subject: [Histonet] Can you help? In-Reply-To: <05e501d30ae6$d9b32af0$8d1980d0$@earthlink.net> References: <05e501d30ae6$d9b32af0$8d1980d0$@earthlink.net> Message-ID: Pam, Do you have any insight as to why the Modesto position has been vacant for so long? I?m not coming out of retirement, but just curious if it is pay or working conditions, etc? It isn?t only the supervisor slot, but there has been a tech position open as well. My brother lives in Modesto. Victor Sent from Mail for Windows 10 From: Pam Barker via Histonet Sent: Tuesday, August 1, 2017 9:55 AM To: Histonetters Histonet Subject: [Histonet] Can you help? Hi Histonetters, How are you? I hope you are having a great week! I was hoping you might be able to help me. I am presently working with several clients throughout the state of California in need of histotechs and histology supervisors. These are full time permanent positions. My clients offer competitive pay, relocation assistance and great teams to work with. They are ready to interview and hire NOW! Here Are The Opportunities: Histology Supervisor - Modesto, CA Histology Supervisor - Irvine, CA Lead Histotech - Los Angeles, CA Grossing Histotech - San Diego Histotech - Los Angeles The help I need from you is do you know anyone that might be interested in hearing about either of these opportunities? If so could you please forward my e-mail to them? If you are interested in this position please contact me ASAP toll free at 866-607-3542, cell/text 407-353-5070 or via email at relia1 at earthlink.net If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won't release it to anyone without your permission. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 at gmail.com Tue Aug 1 17:24:28 2017 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Tue, 1 Aug 2017 15:24:28 -0700 Subject: [Histonet] Fwd: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 References: <223f01d30b04$f1dccaf0$d59660d0$@earthlink.net> Message-ID: Pam: No one in their right mind would work in that lab in Modesto! They have a horrible track record for numerous years! Run and don?t even think about applying! Akemi > Begin forwarded message: > > From: "Pam Barker" > Subject: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 > Date: August 1, 2017 at 1:26:51 PM PDT > To: "Akemi Allison" > > Hi Akemi! > What are you doing on your summer vacation? > Been anyplace you would like to move to? > Whatever you want to do, wherever you want to be, I can help! > > There is still time this summer to make that move. > I have opportunities in all kinds of labs in all areas of the country. > Your next position is just a phone call or an e-mail away. > > All of the opportunities that I represent are permanent full time positions with companies that offer excellent salaries, benefits and relocation assistance. > > Here are some of the opportunities that I am most excited about: > > HISTOLOGY SUPERVISORS AND MANAGERS: > Histology Lab Manager ? Atlanta, GA > Histology Supervisor ? Atlanta, GA > Histology Supervisor ? Boston, MA > Histology Supervisor ? Irvine, CA > Histology Supervisor ? Modesto, CA > Lead Histotech ? Los Angeles ? BRAND NEW LAB > Study Director ? Fort Collins CO > HISTOTECHNICIANS/HISTOTECHNOLOGISTS > CA ? San Diego ? brand new lab CLIA qualified to gross > CA ? Irvine ? learn IHC > CA ? Los Angeles ? Lead Histotech Brand New Lab! > TX ? Nacogdoches day shift hospital environment > CO ? Glenwood day shift hospital environment > TN ? Chattanooga ? CLIA qualified to gross 3rd shift generous shift diff. > MA ? Greater Boston Area - 2nd and 3rd shift generous shift diff. > > If you happen to know anyone I am also looking for a cytotech in Clearwater, FL > > If you or anyone you know are interested in hearing more about any of these opportunities or have another type of position or area in mind and would like some help in your job search please let me know. Just shoot me an e-mail at relia1 at earthlink.net or give me a call toll free at 866-607-3542 or on my cell at 407-353-5070. > > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > Pam M. Barker > > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1 at earthlink.net > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > www.facebook.com /PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia From akemiat3377 at gmail.com Tue Aug 1 18:22:48 2017 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Tue, 1 Aug 2017 16:22:48 -0700 Subject: [Histonet] RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 In-Reply-To: References: <223f01d30b04$f1dccaf0$d59660d0$@earthlink.net> Message-ID: <4709C82B-D90C-4C08-AABC-7524E2AD4FB0@gmail.com> Tim: I know full well about that facility and have known about their activity for several years! I am at a point in my life I really could care less about any consequences! I am Too old to care about being PC when it comes to the care and nurturing of histologists! I am 67 and all about patient care and what we do to do our best to produce the best quality we can! What can anyone do to me at this point in my life??? Akemi Allison-Tacha BS, HT/HTL ASCP (HT 1968/HTL 1982) > On Aug 1, 2017, at 4:09 PM, Morken, Timothy wrote: > > OOO, better watch it, that could get some people mad!! > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > -----Original Message----- > From: Eileen Akemi Allison via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, August 01, 2017 3:24 PM > To: Pam Barker; Histonet > Subject: [Histonet] Fwd: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 > > Pam: No one in their right mind would work in that lab in Modesto! They have a horrible track record for numerous years! Run and don?t even think about applying! > Akemi > >> Begin forwarded message: >> >> From: "Pam Barker" >> Subject: RELIA Histology Careers Bulletin - Live where you vacation >> 8-1-2017 >> Date: August 1, 2017 at 1:26:51 PM PDT >> To: "Akemi Allison" >> >> Hi Akemi! >> What are you doing on your summer vacation? >> Been anyplace you would like to move to? >> Whatever you want to do, wherever you want to be, I can help! >> >> There is still time this summer to make that move. >> I have opportunities in all kinds of labs in all areas of the country. >> Your next position is just a phone call or an e-mail away. >> >> All of the opportunities that I represent are permanent full time positions with companies that offer excellent salaries, benefits and relocation assistance. >> >> Here are some of the opportunities that I am most excited about: >> >> HISTOLOGY SUPERVISORS AND MANAGERS: >> Histology Lab Manager ? Atlanta, GA >> Histology Supervisor ? Atlanta, GA >> Histology Supervisor ? Boston, MA >> Histology Supervisor ? Irvine, CA >> Histology Supervisor ? Modesto, CA >> Lead Histotech ? Los Angeles ? BRAND NEW LAB Study Director ? Fort >> Collins CO HISTOTECHNICIANS/HISTOTECHNOLOGISTS >> CA ? San Diego ? brand new lab CLIA qualified to gross CA ? Irvine ? >> learn IHC CA ? Los Angeles ? Lead Histotech Brand New Lab! >> TX ? Nacogdoches day shift hospital environment CO ? Glenwood day >> shift hospital environment TN ? Chattanooga ? CLIA qualified to gross >> 3rd shift generous shift diff. >> MA ? Greater Boston Area - 2nd and 3rd shift generous shift diff. >> >> If you happen to know anyone I am also looking for a cytotech in >> Clearwater, FL >> >> If you or anyone you know are interested in hearing more about any of these opportunities or have another type of position or area in mind and would like some help in your job search please let me know. Just shoot me an e-mail at relia1 at earthlink.net or give me a call toll free at 866-607-3542 or on my cell at 407-353-5070. >> >> Thanks-Pam >> >> Right Place, Right Time, Right Move with RELIA! >> >> Thank You! >> Pam M. Barker >> >> Pam Barker >> President/Senior Recruiting Specialist-Histology RELIA Solutions >> Specialists in Allied Healthcare Recruiting >> 5703 Red Bug Lake Road #330 >> Winter Springs, FL 32708-4969 >> Phone: (407)657-2027 >> Cell: (407)353-5070 >> FAX: (407)678-2788 >> E-mail: relia1 at earthlink.net >> https://www.facebook.com/RELIASolutionsforhistologyprofessionals >> >> www.facebook.com >> /PamBarkerRELIA >> www.linkedin.com/in/reliasolutions >> >> www.twitter.com/pamatrelia > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Tue Aug 1 18:33:57 2017 From: patpxs at gmail.com (P Sicurello) Date: Tue, 1 Aug 2017 16:33:57 -0700 Subject: [Histonet] Fwd: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 In-Reply-To: References: <223f01d30b04$f1dccaf0$d59660d0$@earthlink.net> Message-ID: Who would want to vacation in Modesto? Not me! Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Tue, Aug 1, 2017 at 3:24 PM, Eileen Akemi Allison via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Pam: No one in their right mind would work in that lab in Modesto! They > have a horrible track record for numerous years! Run and don?t even think > about applying! > Akemi > > > Begin forwarded message: > > > > From: "Pam Barker" > > Subject: RELIA Histology Careers Bulletin - Live where you vacation > 8-1-2017 > > Date: August 1, 2017 at 1:26:51 PM PDT > > To: "Akemi Allison" > > > > Hi Akemi! > > What are you doing on your summer vacation? > > Been anyplace you would like to move to? > > Whatever you want to do, wherever you want to be, I can help! > > > > There is still time this summer to make that move. > > I have opportunities in all kinds of labs in all areas of the country. > > Your next position is just a phone call or an e-mail away. > > > > All of the opportunities that I represent are permanent full time > positions with companies that offer excellent salaries, benefits and > relocation assistance. > > > > Here are some of the opportunities that I am most excited about: > > > > HISTOLOGY SUPERVISORS AND MANAGERS: > > Histology Lab Manager ? Atlanta, GA > > Histology Supervisor ? Atlanta, GA > > Histology Supervisor ? Boston, MA > > Histology Supervisor ? Irvine, CA > > Histology Supervisor ? Modesto, CA > > Lead Histotech ? Los Angeles ? BRAND NEW LAB > > Study Director ? Fort Collins CO > > HISTOTECHNICIANS/HISTOTECHNOLOGISTS > > CA ? San Diego ? brand new lab CLIA qualified to gross > > CA ? Irvine ? learn IHC > > CA ? Los Angeles ? Lead Histotech Brand New Lab! > > TX ? Nacogdoches day shift hospital environment > > CO ? Glenwood day shift hospital environment > > TN ? Chattanooga ? CLIA qualified to gross 3rd shift generous shift diff. > > MA ? Greater Boston Area - 2nd and 3rd shift generous shift diff. > > > > If you happen to know anyone I am also looking for a cytotech in > Clearwater, FL > > > > If you or anyone you know are interested in hearing more about any of > these opportunities or have another type of position or area in mind and > would like some help in your job search please let me know. Just shoot me > an e-mail at relia1 at earthlink.net or give > me a call toll free at 866-607-3542 or on my cell at 407-353-5070. > > > > Thanks-Pam > > > > Right Place, Right Time, Right Move with RELIA! > > > > Thank You! > > Pam M. Barker > > > > Pam Barker > > President/Senior Recruiting Specialist-Histology > > RELIA Solutions > > Specialists in Allied Healthcare Recruiting > > 5703 Red Bug Lake Road #330 > > Winter Springs, FL 32708-4969 > > Phone: (407)657-2027 > > Cell: (407)353-5070 > > FAX: (407)678-2788 > > E-mail: relia1 at earthlink.net > > https://www.facebook.com/RELIASolutionsforhistologyprofessionals < > https://www.facebook.com/RELIASolutionsforhistologyprofessionals> > > www.facebook.com /PamBarkerRELIA > > www.linkedin.com/in/reliasolutions reliasolutions> > > www.twitter.com/pamatrelia > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 at earthlink.net Wed Aug 2 08:32:21 2017 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 2 Aug 2017 09:32:21 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 Message-ID: <00c701d30b93$c57c44b0$5074ce10$@earthlink.net> Hi Histonetters! I just want to take a minute to address the opinion expressed about one of the employers on my most recent email. I apologize that there was editorializing on a particular lab and I just want to point out after over 30 years in recruiting in all types of environments in all fairness: The quality of the workplace is in the eye of the beholder. In other words not every place is right for everybody. Not every place is right for everyone but I promise that I will work my hardest to find you the right position in the right place. Thanks again - Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From patpxs at gmail.com Wed Aug 2 08:53:42 2017 From: patpxs at gmail.com (Patpxs) Date: Wed, 2 Aug 2017 06:53:42 -0700 Subject: [Histonet] RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 In-Reply-To: <00c701d30b93$c57c44b0$5074ce10$@earthlink.net> References: <00c701d30b93$c57c44b0$5074ce10$@earthlink.net> Message-ID: <22A2426F-53EA-4532-86A9-BBC259C757D4@gmail.com> My comment was meant in jest. I live in San Diego, so Modesto is not a city I would vacation. Modesto probably has many nice features. Paula Sent from my iPhone > On Aug 2, 2017, at 6:32 AM, Pam Barker via Histonet wrote: > > Hi Histonetters! > I just want to take a minute to address the opinion expressed about one of > the employers on my most recent email. > I apologize that there was editorializing on a particular lab and I just > want to point out after over 30 years in recruiting in all types of > environments in all fairness: > The quality of the workplace is in the eye of the beholder. > In other words not every place is right for everybody. > Not every place is right for everyone but I promise that I will work my > hardest to find you the right position in the right place. > > Thanks again - Pam > > > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > Pam M. Barker > > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1 at earthlink.net > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > www.facebook.com /PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed Aug 2 08:58:28 2017 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 2 Aug 2017 09:58:28 -0400 (GMT-04:00) Subject: [Histonet] RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 Message-ID: <5317598.1059.1501682308979@wamui-junio.atl.sa.earthlink.net> No worries Paula, I totally got you! I was referring to another post. xoxoxo -----Original Message----- >From: Patpxs via Histonet >Sent: Aug 2, 2017 9:53 AM >To: Pam Barker , histonet at lists.utsouthwestern.edu >Subject: Re: [Histonet] RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 > >My comment was meant in jest. I live in San Diego, so Modesto is not a city I would vacation. Modesto probably has many nice features. > >Paula > >Sent from my iPhone > >> On Aug 2, 2017, at 6:32 AM, Pam Barker via Histonet wrote: >> >> Hi Histonetters! >> I just want to take a minute to address the opinion expressed about one of >> the employers on my most recent email. >> I apologize that there was editorializing on a particular lab and I just >> want to point out after over 30 years in recruiting in all types of >> environments in all fairness: >> The quality of the workplace is in the eye of the beholder. >> In other words not every place is right for everybody. >> Not every place is right for everyone but I promise that I will work my >> hardest to find you the right position in the right place. >> >> Thanks again - Pam >> >> >> Thanks-Pam >> >> Right Place, Right Time, Right Move with RELIA! >> >> Thank You! >> Pam M. Barker >> >> Pam Barker >> President/Senior Recruiting Specialist-Histology >> RELIA Solutions >> Specialists in Allied Healthcare Recruiting >> 5703 Red Bug Lake Road #330 >> Winter Springs, FL 32708-4969 >> Phone: (407)657-2027 >> Cell: (407)353-5070 >> FAX: (407)678-2788 >> E-mail: relia1 at earthlink.net >> https://www.facebook.com/RELIASolutionsforhistologyprofessionals >> www.facebook.com /PamBarkerRELIA >> www.linkedin.com/in/reliasolutions >> www.twitter.com/pamatrelia >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Thank you! Pam M. Barker President RELIA 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: 407-657-2027 Fax: 407-678-2788 Cell: 407-353-5070 e-mail: relia1 at earthlink.net Toll Free: 866-60-RELIA (866-607-3542) From melissa at alliedsearchpartners.com Wed Aug 2 08:59:52 2017 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Wed, 2 Aug 2017 13:59:52 +0000 Subject: [Histonet] RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 In-Reply-To: <00c701d30b93$c57c44b0$5074ce10$@earthlink.net> References: <00c701d30b93$c57c44b0$5074ce10$@earthlink.net> Message-ID: Well said Pam! Keep up the good work. Melissa Owens President, Laboratory Staffing Allied Search Partners Direct Line: 407.413.9117 Toll Free: 888.388.7571 ext. 102 F: 888.388.7572 On 8/2/17, 9:32 AM, "Pam Barker via Histonet" wrote: >Hi Histonetters! >I just want to take a minute to address the opinion expressed about one of >the employers on my most recent email. >I apologize that there was editorializing on a particular lab and I just >want to point out after over 30 years in recruiting in all types of >environments in all fairness: >The quality of the workplace is in the eye of the beholder. >In other words not every place is right for everybody. >Not every place is right for everyone but I promise that I will work my >hardest to find you the right position in the right place. > >Thanks again - Pam > > >Thanks-Pam > >Right Place, Right Time, Right Move with RELIA! > >Thank You! > Pam M. Barker > >Pam Barker >President/Senior Recruiting Specialist-Histology >RELIA Solutions >Specialists in Allied Healthcare Recruiting >5703 Red Bug Lake Road #330 >Winter Springs, FL 32708-4969 >Phone: (407)657-2027 >Cell: (407)353-5070 >FAX: (407)678-2788 >E-mail: relia1 at earthlink.net >https://www.facebook.com/RELIASolutionsforhistologyprofessionals >www.facebook.com /PamBarkerRELIA >www.linkedin.com/in/reliasolutions >www.twitter.com/pamatrelia > > > > > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt at pa-ucl.com Wed Aug 2 13:49:47 2017 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Wed, 2 Aug 2017 18:49:47 +0000 Subject: [Histonet] pre cut control slides Message-ID: Hello- How are you handling precut control slides for IHC stains? How long do you keep them before throwing? I found some info (on histonet) on special stains but not on IHC stains. Thanks Nancy Schmitt HT, MLT(ASCP) United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Pat.Patterson at propath.com Wed Aug 2 21:04:39 2017 From: Pat.Patterson at propath.com (Pat Patterson) Date: Thu, 3 Aug 2017 02:04:39 +0000 Subject: [Histonet] Histonet Digest, Vol 165, Issue 2 In-Reply-To: References: Message-ID: <6DCB8B92D0138244B56CE8EACE0D458D4FFD3B3D@Mail.propathlab.com> I think this conversation should be taken off-line. Histonet was not meant for this. Technical help was priority. Pat ________________________________________ From: histonet-request at lists.utsouthwestern.edu [histonet-request at lists.utsouthwestern.edu] Sent: Wednesday, August 02, 2017 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 165, Issue 2 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Can you help? (victor_tobias at comcast.net) 2. Fwd: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 (Eileen Akemi Allison) 3. Re: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 (Eileen Akemi Allison) 4. Re: Fwd: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 (P Sicurello) 5. Re: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 (Pam Barker) 6. Re: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 (Patpxs) 7. Re: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 (Pam Barker) 8. Re: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 (Melissa Owens) ---------------------------------------------------------------------- Message: 1 Date: Tue, 1 Aug 2017 14:14:52 -0700 From: To: Pam Barker via Histonet Subject: Re: [Histonet] Can you help? Message-ID: Content-Type: text/plain; charset="utf-8" Pam, Do you have any insight as to why the Modesto position has been vacant for so long? I?m not coming out of retirement, but just curious if it is pay or working conditions, etc? It isn?t only the supervisor slot, but there has been a tech position open as well. My brother lives in Modesto. Victor Sent from Mail for Windows 10 From: Pam Barker via Histonet Sent: Tuesday, August 1, 2017 9:55 AM To: Histonetters Histonet Subject: [Histonet] Can you help? Hi Histonetters, How are you? I hope you are having a great week! I was hoping you might be able to help me. I am presently working with several clients throughout the state of California in need of histotechs and histology supervisors. These are full time permanent positions. My clients offer competitive pay, relocation assistance and great teams to work with. They are ready to interview and hire NOW! Here Are The Opportunities: Histology Supervisor - Modesto, CA Histology Supervisor - Irvine, CA Lead Histotech - Los Angeles, CA Grossing Histotech - San Diego Histotech - Los Angeles The help I need from you is do you know anyone that might be interested in hearing about either of these opportunities? If so could you please forward my e-mail to them? If you are interested in this position please contact me ASAP toll free at 866-607-3542, cell/text 407-353-5070 or via email at relia1 at earthlink.net If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won't release it to anyone without your permission. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 1 Aug 2017 15:24:28 -0700 From: Eileen Akemi Allison To: Pam Barker , Histonet Subject: [Histonet] Fwd: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 Message-ID: Content-Type: text/plain; charset=utf-8 Pam: No one in their right mind would work in that lab in Modesto! They have a horrible track record for numerous years! Run and don?t even think about applying! Akemi > Begin forwarded message: > > From: "Pam Barker" > Subject: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 > Date: August 1, 2017 at 1:26:51 PM PDT > To: "Akemi Allison" > > Hi Akemi! > What are you doing on your summer vacation? > Been anyplace you would like to move to? > Whatever you want to do, wherever you want to be, I can help! > > There is still time this summer to make that move. > I have opportunities in all kinds of labs in all areas of the country. > Your next position is just a phone call or an e-mail away. > > All of the opportunities that I represent are permanent full time positions with companies that offer excellent salaries, benefits and relocation assistance. > > Here are some of the opportunities that I am most excited about: > > HISTOLOGY SUPERVISORS AND MANAGERS: > Histology Lab Manager ? Atlanta, GA > Histology Supervisor ? Atlanta, GA > Histology Supervisor ? Boston, MA > Histology Supervisor ? Irvine, CA > Histology Supervisor ? Modesto, CA > Lead Histotech ? Los Angeles ? BRAND NEW LAB > Study Director ? Fort Collins CO > HISTOTECHNICIANS/HISTOTECHNOLOGISTS > CA ? San Diego ? brand new lab CLIA qualified to gross > CA ? Irvine ? learn IHC > CA ? Los Angeles ? Lead Histotech Brand New Lab! > TX ? Nacogdoches day shift hospital environment > CO ? Glenwood day shift hospital environment > TN ? Chattanooga ? CLIA qualified to gross 3rd shift generous shift diff. > MA ? Greater Boston Area - 2nd and 3rd shift generous shift diff. > > If you happen to know anyone I am also looking for a cytotech in Clearwater, FL > > If you or anyone you know are interested in hearing more about any of these opportunities or have another type of position or area in mind and would like some help in your job search please let me know. Just shoot me an e-mail at relia1 at earthlink.net or give me a call toll free at 866-607-3542 or on my cell at 407-353-5070. > > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > Pam M. Barker > > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1 at earthlink.net > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > www.facebook.com /PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia ------------------------------ Message: 3 Date: Tue, 1 Aug 2017 16:22:48 -0700 From: Eileen Akemi Allison To: "Morken, Timothy" , Histonet Subject: Re: [Histonet] RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 Message-ID: <4709C82B-D90C-4C08-AABC-7524E2AD4FB0 at gmail.com> Content-Type: text/plain; charset=utf-8 Tim: I know full well about that facility and have known about their activity for several years! I am at a point in my life I really could care less about any consequences! I am Too old to care about being PC when it comes to the care and nurturing of histologists! I am 67 and all about patient care and what we do to do our best to produce the best quality we can! What can anyone do to me at this point in my life??? Akemi Allison-Tacha BS, HT/HTL ASCP (HT 1968/HTL 1982) > On Aug 1, 2017, at 4:09 PM, Morken, Timothy wrote: > > OOO, better watch it, that could get some people mad!! > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > -----Original Message----- > From: Eileen Akemi Allison via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, August 01, 2017 3:24 PM > To: Pam Barker; Histonet > Subject: [Histonet] Fwd: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 > > Pam: No one in their right mind would work in that lab in Modesto! They have a horrible track record for numerous years! Run and don?t even think about applying! > Akemi > >> Begin forwarded message: >> >> From: "Pam Barker" >> Subject: RELIA Histology Careers Bulletin - Live where you vacation >> 8-1-2017 >> Date: August 1, 2017 at 1:26:51 PM PDT >> To: "Akemi Allison" >> >> Hi Akemi! >> What are you doing on your summer vacation? >> Been anyplace you would like to move to? >> Whatever you want to do, wherever you want to be, I can help! >> >> There is still time this summer to make that move. >> I have opportunities in all kinds of labs in all areas of the country. >> Your next position is just a phone call or an e-mail away. >> >> All of the opportunities that I represent are permanent full time positions with companies that offer excellent salaries, benefits and relocation assistance. >> >> Here are some of the opportunities that I am most excited about: >> >> HISTOLOGY SUPERVISORS AND MANAGERS: >> Histology Lab Manager ? Atlanta, GA >> Histology Supervisor ? Atlanta, GA >> Histology Supervisor ? Boston, MA >> Histology Supervisor ? Irvine, CA >> Histology Supervisor ? Modesto, CA >> Lead Histotech ? Los Angeles ? BRAND NEW LAB Study Director ? Fort >> Collins CO HISTOTECHNICIANS/HISTOTECHNOLOGISTS >> CA ? San Diego ? brand new lab CLIA qualified to gross CA ? Irvine ? >> learn IHC CA ? Los Angeles ? Lead Histotech Brand New Lab! >> TX ? Nacogdoches day shift hospital environment CO ? Glenwood day >> shift hospital environment TN ? Chattanooga ? CLIA qualified to gross >> 3rd shift generous shift diff. >> MA ? Greater Boston Area - 2nd and 3rd shift generous shift diff. >> >> If you happen to know anyone I am also looking for a cytotech in >> Clearwater, FL >> >> If you or anyone you know are interested in hearing more about any of these opportunities or have another type of position or area in mind and would like some help in your job search please let me know. Just shoot me an e-mail at relia1 at earthlink.net or give me a call toll free at 866-607-3542 or on my cell at 407-353-5070. >> >> Thanks-Pam >> >> Right Place, Right Time, Right Move with RELIA! >> >> Thank You! >> Pam M. Barker >> >> Pam Barker >> President/Senior Recruiting Specialist-Histology RELIA Solutions >> Specialists in Allied Healthcare Recruiting >> 5703 Red Bug Lake Road #330 >> Winter Springs, FL 32708-4969 >> Phone: (407)657-2027 >> Cell: (407)353-5070 >> FAX: (407)678-2788 >> E-mail: relia1 at earthlink.net >> https://www.facebook.com/RELIASolutionsforhistologyprofessionals >> >> www.facebook.com >> /PamBarkerRELIA >> www.linkedin.com/in/reliasolutions >> >> www.twitter.com/pamatrelia > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 1 Aug 2017 16:33:57 -0700 From: P Sicurello To: Eileen Akemi Allison Cc: Pam Barker , Histonet Subject: Re: [Histonet] Fwd: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 Message-ID: Content-Type: text/plain; charset="UTF-8" Who would want to vacation in Modesto? Not me! Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Tue, Aug 1, 2017 at 3:24 PM, Eileen Akemi Allison via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Pam: No one in their right mind would work in that lab in Modesto! They > have a horrible track record for numerous years! Run and don?t even think > about applying! > Akemi > > > Begin forwarded message: > > > > From: "Pam Barker" > > Subject: RELIA Histology Careers Bulletin - Live where you vacation > 8-1-2017 > > Date: August 1, 2017 at 1:26:51 PM PDT > > To: "Akemi Allison" > > > > Hi Akemi! > > What are you doing on your summer vacation? > > Been anyplace you would like to move to? > > Whatever you want to do, wherever you want to be, I can help! > > > > There is still time this summer to make that move. > > I have opportunities in all kinds of labs in all areas of the country. > > Your next position is just a phone call or an e-mail away. > > > > All of the opportunities that I represent are permanent full time > positions with companies that offer excellent salaries, benefits and > relocation assistance. > > > > Here are some of the opportunities that I am most excited about: > > > > HISTOLOGY SUPERVISORS AND MANAGERS: > > Histology Lab Manager ? Atlanta, GA > > Histology Supervisor ? Atlanta, GA > > Histology Supervisor ? Boston, MA > > Histology Supervisor ? Irvine, CA > > Histology Supervisor ? Modesto, CA > > Lead Histotech ? Los Angeles ? BRAND NEW LAB > > Study Director ? Fort Collins CO > > HISTOTECHNICIANS/HISTOTECHNOLOGISTS > > CA ? San Diego ? brand new lab CLIA qualified to gross > > CA ? Irvine ? learn IHC > > CA ? Los Angeles ? Lead Histotech Brand New Lab! > > TX ? Nacogdoches day shift hospital environment > > CO ? Glenwood day shift hospital environment > > TN ? Chattanooga ? CLIA qualified to gross 3rd shift generous shift diff. > > MA ? Greater Boston Area - 2nd and 3rd shift generous shift diff. > > > > If you happen to know anyone I am also looking for a cytotech in > Clearwater, FL > > > > If you or anyone you know are interested in hearing more about any of > these opportunities or have another type of position or area in mind and > would like some help in your job search please let me know. Just shoot me > an e-mail at relia1 at earthlink.net or give > me a call toll free at 866-607-3542 or on my cell at 407-353-5070. > > > > Thanks-Pam > > > > Right Place, Right Time, Right Move with RELIA! > > > > Thank You! > > Pam M. Barker > > > > Pam Barker > > President/Senior Recruiting Specialist-Histology > > RELIA Solutions > > Specialists in Allied Healthcare Recruiting > > 5703 Red Bug Lake Road #330 > > Winter Springs, FL 32708-4969 > > Phone: (407)657-2027 > > Cell: (407)353-5070 > > FAX: (407)678-2788 > > E-mail: relia1 at earthlink.net > > https://www.facebook.com/RELIASolutionsforhistologyprofessionals < > https://www.facebook.com/RELIASolutionsforhistologyprofessionals> > > www.facebook.com /PamBarkerRELIA > > www.linkedin.com/in/reliasolutions reliasolutions> > > www.twitter.com/pamatrelia > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 5 Date: Wed, 2 Aug 2017 09:32:21 -0400 From: "Pam Barker" To: "Histonetters Histonet" Subject: Re: [Histonet] RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 Message-ID: <00c701d30b93$c57c44b0$5074ce10$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters! I just want to take a minute to address the opinion expressed about one of the employers on my most recent email. I apologize that there was editorializing on a particular lab and I just want to point out after over 30 years in recruiting in all types of environments in all fairness: The quality of the workplace is in the eye of the beholder. In other words not every place is right for everybody. Not every place is right for everyone but I promise that I will work my hardest to find you the right position in the right place. Thanks again - Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 6 Date: Wed, 2 Aug 2017 06:53:42 -0700 From: Patpxs To: Pam Barker , histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 Message-ID: <22A2426F-53EA-4532-86A9-BBC259C757D4 at gmail.com> Content-Type: text/plain; charset=us-ascii My comment was meant in jest. I live in San Diego, so Modesto is not a city I would vacation. Modesto probably has many nice features. Paula Sent from my iPhone > On Aug 2, 2017, at 6:32 AM, Pam Barker via Histonet wrote: > > Hi Histonetters! > I just want to take a minute to address the opinion expressed about one of > the employers on my most recent email. > I apologize that there was editorializing on a particular lab and I just > want to point out after over 30 years in recruiting in all types of > environments in all fairness: > The quality of the workplace is in the eye of the beholder. > In other words not every place is right for everybody. > Not every place is right for everyone but I promise that I will work my > hardest to find you the right position in the right place. > > Thanks again - Pam > > > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > Pam M. Barker > > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1 at earthlink.net > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > www.facebook.com /PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 2 Aug 2017 09:58:28 -0400 (GMT-04:00) From: Pam Barker To: Patpxs , histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 Message-ID: <5317598.1059.1501682308979 at wamui-junio.atl.sa.earthlink.net> Content-Type: text/plain; charset=UTF-8 No worries Paula, I totally got you! I was referring to another post. xoxoxo -----Original Message----- >From: Patpxs via Histonet >Sent: Aug 2, 2017 9:53 AM >To: Pam Barker , histonet at lists.utsouthwestern.edu >Subject: Re: [Histonet] RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 > >My comment was meant in jest. I live in San Diego, so Modesto is not a city I would vacation. Modesto probably has many nice features. > >Paula > >Sent from my iPhone > >> On Aug 2, 2017, at 6:32 AM, Pam Barker via Histonet wrote: >> >> Hi Histonetters! >> I just want to take a minute to address the opinion expressed about one of >> the employers on my most recent email. >> I apologize that there was editorializing on a particular lab and I just >> want to point out after over 30 years in recruiting in all types of >> environments in all fairness: >> The quality of the workplace is in the eye of the beholder. >> In other words not every place is right for everybody. >> Not every place is right for everyone but I promise that I will work my >> hardest to find you the right position in the right place. >> >> Thanks again - Pam >> >> >> Thanks-Pam >> >> Right Place, Right Time, Right Move with RELIA! >> >> Thank You! >> Pam M. Barker >> >> Pam Barker >> President/Senior Recruiting Specialist-Histology >> RELIA Solutions >> Specialists in Allied Healthcare Recruiting >> 5703 Red Bug Lake Road #330 >> Winter Springs, FL 32708-4969 >> Phone: (407)657-2027 >> Cell: (407)353-5070 >> FAX: (407)678-2788 >> E-mail: relia1 at earthlink.net >> https://www.facebook.com/RELIASolutionsforhistologyprofessionals >> www.facebook.com /PamBarkerRELIA >> www.linkedin.com/in/reliasolutions >> www.twitter.com/pamatrelia >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Thank you! Pam M. Barker President RELIA 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: 407-657-2027 Fax: 407-678-2788 Cell: 407-353-5070 e-mail: relia1 at earthlink.net Toll Free: 866-60-RELIA (866-607-3542) ------------------------------ Message: 8 Date: Wed, 2 Aug 2017 13:59:52 +0000 From: Melissa Owens To: Pam Barker , Histonetters Histonet Subject: Re: [Histonet] RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017 Message-ID: Content-Type: text/plain; charset="us-ascii" Well said Pam! Keep up the good work. Melissa Owens President, Laboratory Staffing Allied Search Partners Direct Line: 407.413.9117 Toll Free: 888.388.7571 ext. 102 F: 888.388.7572 On 8/2/17, 9:32 AM, "Pam Barker via Histonet" wrote: >Hi Histonetters! >I just want to take a minute to address the opinion expressed about one of >the employers on my most recent email. >I apologize that there was editorializing on a particular lab and I just >want to point out after over 30 years in recruiting in all types of >environments in all fairness: >The quality of the workplace is in the eye of the beholder. >In other words not every place is right for everybody. >Not every place is right for everyone but I promise that I will work my >hardest to find you the right position in the right place. > >Thanks again - Pam > > >Thanks-Pam > >Right Place, Right Time, Right Move with RELIA! > >Thank You! > Pam M. Barker > >Pam Barker >President/Senior Recruiting Specialist-Histology >RELIA Solutions >Specialists in Allied Healthcare Recruiting >5703 Red Bug Lake Road #330 >Winter Springs, FL 32708-4969 >Phone: (407)657-2027 >Cell: (407)353-5070 >FAX: (407)678-2788 >E-mail: relia1 at earthlink.net >https://www.facebook.com/RELIASolutionsforhistologyprofessionals >www.facebook.com /PamBarkerRELIA >www.linkedin.com/in/reliasolutions >www.twitter.com/pamatrelia > > > > > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 165, Issue 2 **************************************** This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From Kim.Kolman at va.gov Thu Aug 3 11:05:04 2017 From: Kim.Kolman at va.gov (Kolman, Kimberly D.) Date: Thu, 3 Aug 2017 16:05:04 +0000 Subject: [Histonet] baskets for VIP processor Message-ID: <20F3E9AEDD1CB147B4BCC21E62E207E93F928267@VAPNSMSGD53S03.vha.med.va.gov> Has anyone noticed the stainless steel baskets for a Sakura VIP-6 tissue processor warping on the sides? Still perfectly useable; mine just start bowing out on each side. They've not been dropped. It does leave a small gap on each side that I suppose a cassette could sneak out of. I occasionally pound them back into shape and they are fine. Thanks, Kim Kimberly D. Kolman, HT (ASCP) VA Eastern Kansas Health Care System Dwight D. Eisenhower VA Medical Center 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 913-682-2000 x 52537 Fax: 913-758-4193 Kim.kolman at va.gov From relia1 at earthlink.net Thu Aug 3 11:05:47 2017 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 3 Aug 2017 12:05:47 -0400 Subject: [Histonet] Can you help? New position in GA to tell you about. Message-ID: <025701d30c72$603c3580$20b4a080$@earthlink.net> Hello Histonetters, I hope you are having a great week. And are looking forward to a fantastic weekend. I have a new position to tell you about in Atlanta. If you or anyone you know might be interested, when you get a chance please call me. If I place someone you refer You will earn a referral fee! I can be reached toll free at the office at 866-607-3542 or relia1 at earthlink.net or you can always catch me on cell via call or text at 407-353-5070. *p.s. Just a friendly reminder out of respect to the other members of the histonet to direct all responses to me directly at relia1 at earthlink.net as opposed to the histonet board. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From AJohnson at aipathology.com Thu Aug 3 13:27:41 2017 From: AJohnson at aipathology.com (Amy Johnson) Date: Thu, 3 Aug 2017 18:27:41 +0000 Subject: [Histonet] Who can perform high complexity testing Message-ID: What kind of credentials does one need to perform High Complexity testing? My understanding was if you had a 4 year degree of some kind (with some science/biology background) you are qualified to perform these types of tests. Others have said if you don't have a degree but have your certification as a HT or MLT you are qualified as well........ Thanks for clarifying, Amylin Johnson, B.S. HTL(ASCP) Associates in Pathology Wausau Wi 54401 715-847-2130 From wbenton at cua.md Thu Aug 3 13:56:48 2017 From: wbenton at cua.md (Walter Benton) Date: Thu, 3 Aug 2017 18:56:48 +0000 Subject: [Histonet] Who can perform high complexity testing In-Reply-To: References: Message-ID: Amy, CLIA 88 Guidelines will answer your questions, but here are some PDFs that may helpful as well. Certifications are not listed in the CLIA guidelines, but educational achievements (e.g. degrees earned and/or college credits earned in specific disciplines may qualify an individual). http://www.jointcommission.org/assets/1/18/clia_required_personnel_qualifications.pdf http://www.cap.org/ShowProperty?nodePath=/UCMCon/Contribution%20Folders/DctmContent/education/OnlineCourseContent/2014/TLTM/TLTM_Preinsp_personnelrequirementsbytestingcomplexity.pdf CFR > Title 42 > Chapter IV > Subchapter G > Part 493 > Subpart M > Section 493.1489 42 CFR 493.1489 - Standard; Testing personnel qualifications. eCFR Authorities (U.S. Code) What Cites Me prev | next ? 493.1489 Standard; Testing personnel qualifications. Each individual performing high complexity testing must - (a) Possess a current license issued by the State in which the laboratory is located, if such licensing is required; and (b) Meet one of the following requirements: (1) Be a doctor of medicine, doctor of osteopathy, or doctor of podiatric medicine licensed to practice medicine, osteopathy, or podiatry in the State in which the laboratory is located or have earned a doctoral, master's or bachelor's degree in a chemical, physical, biological or clinical laboratory science, or medical technology from an accredited institution; (2) (i) Have earned an associate degree in a laboratory science, or medical laboratory technology from an accredited institution or - (ii) Have education and training equivalent to that specified in paragraph (b)(2)(i) of this section that includes - (A) At least 60 semester hours, or equivalent, from an accredited institution that, at a minimum, include either - (1) 24 semester hours of medical laboratory technology courses; or (2) 24 semester hours of science courses that include - (i) Six semester hours of chemistry; (ii) Six semester hours of biology; and (iii) Twelve semester hours of chemistry, biology, or medical laboratory technology in any combination; and (B) Have laboratory training that includes either of the following: (1) Completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS. (This training may be included in the 60 semester hours listed in paragraph (b)(2)(ii)(A) of this section.) (2) At least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. (3) Have previously qualified or could have qualified as a technologist under ? 493.1491 on or before February 28, 1992; (4) On or before April 24, 1995 be a high school graduate or equivalent and have either - (i) Graduated from a medical laboratory or clinical laboratory training program approved or accredited by ABHES, CAHEA, or other organization approved by HHS; or (ii) Successfully completed an official U.S. military medical laboratory procedures training course of at least 50 weeks duration and have held the military enlisted occupational specialty of Medical Laboratory Specialist (Laboratory Technician); (5) (i) Until September 1, 1997 - (A) Have earned a high school diploma or equivalent; and (B) Have documentation of training appropriate for the testing performed before analyzing patient specimens. Such training must ensure that the individual has - (1) The skills required for proper specimen collection, including patient preparation, if applicable, labeling, handling, preservation or fixation, processing or preparation, transportation and storage of specimens; (2) The skills required for implementing all standard laboratory procedures; (3) The skills required for performing each test method and for proper instrument use; (4) The skills required for performing preventive maintenance, troubleshooting, and calibration procedures related to each test performed; (5) A working knowledge of reagent stability and storage; (6) The skills required to implement the quality control policies and procedures of the laboratory; (7) An awareness of the factors that influence test results; and (8) The skills required to assess and verify the validity of patient test results through the evaluation of quality control values before reporting patient test results; and (ii) As of September 1, 1997, be qualified under ? 493.1489(b)(1), (b)(2), or (b)(4), except for those individuals qualified under paragraph (b)(5)(i) of this section who were performing high complexity testing on or before April 24, 1995; (6) For blood gas analysis - (i) Be qualified under ? 493.1489(b)(1), (b)(2), (b)(3), (b)(4), or (b)(5); (ii) Have earned a bachelor's degree in respiratory therapy or cardiovascular technology from an accredited institution; or (iii) Have earned an associate degree related to pulmonary function from an accredited institution; or (7) For histopathology, meet the qualifications of ? 493.1449 (b) or (l) to perform tissue examinations. [ 57 FR 7172, Feb. 28, 1992, as amended at 58 FR 5236, Jan. 19, 1993; 58 FR 39155, July 22, 1993; 60 FR 20050, Apr. 24, 1995] Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 410-768-5961 (Lab) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: Amy Johnson via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, August 03, 2017 2:28 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Who can perform high complexity testing What kind of credentials does one need to perform High Complexity testing? My understanding was if you had a 4 year degree of some kind (with some science/biology background) you are qualified to perform these types of tests. Others have said if you don't have a degree but have your certification as a HT or MLT you are qualified as well........ Thanks for clarifying, Amylin Johnson, B.S. HTL(ASCP) Associates in Pathology Wausau Wi 54401 715-847-2130 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From patpxs at gmail.com Thu Aug 3 17:50:05 2017 From: patpxs at gmail.com (P Sicurello) Date: Thu, 3 Aug 2017 15:50:05 -0700 Subject: [Histonet] Adenovirus antibody? Message-ID: Hello Listers, I am listing to starboard on this one. We are using the Ventana Ultra staining platform and have had a bit of trouble with our Adenovirus stains. We have used Abcam and Millipore to no avail. Do you have suggestions about another vendor to try? We are a clinical lab so we only stain human tissue. IVD is preferred but ASR and RUO are OK too. Any suggestions are gratefully accepted. Thank you in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From tony.henwood at health.nsw.gov.au Thu Aug 3 20:47:08 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 4 Aug 2017 01:47:08 +0000 Subject: [Histonet] Adenovirus antibody? In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF955641BC@SVDCMBX-MEX008.nswhealth.net> Hi Paula, We use Adenovirus antibody from BioSB (Diagnostic Technology) Cat Number BSB 5040 Clone 20/11 and 2/6 Isotype IgG1/K Dilution 1:200 with citrate retrieval on the Bond 3 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 4 August 2017 8:50 AM To: HistoNet Subject: [Histonet] Adenovirus antibody? Hello Listers, I am listing to starboard on this one. We are using the Ventana Ultra staining platform and have had a bit of trouble with our Adenovirus stains. We have used Abcam and Millipore to no avail. Do you have suggestions about another vendor to try? We are a clinical lab so we only stain human tissue. IVD is preferred but ASR and RUO are OK too. Any suggestions are gratefully accepted. Thank you in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From LRaff at uropartners.com Fri Aug 4 07:46:37 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 4 Aug 2017 12:46:37 +0000 Subject: [Histonet] Happy Friday--Lab related blog post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF115573F4@COLOEXCH01.uropartners.local> Have a good weekend-and I hope your lab works like ours! http://www.chicagonow.com/downsize-maybe/2017/08/we-are-great-because-you-wont-stop/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From shive003 at umn.edu Fri Aug 4 09:46:09 2017 From: shive003 at umn.edu (Jan Shivers) Date: Fri, 4 Aug 2017 09:46:09 -0500 Subject: [Histonet] Adenovirus antibody? In-Reply-To: <0237449DE79DBC45B686AB82CDCD16FF955641BC@SVDCMBX-MEX008.nswhealth.net> References: <0237449DE79DBC45B686AB82CDCD16FF955641BC@SVDCMBX-MEX008.nswhealth.net> Message-ID: Hello Paula, This isn't an answer to your question, because I do use the Millipore antibody (MAB805) and it works quite well on a Dako platform or done manually. It requires enzyme digestion antigen retrieval. I use it at a 1:400 dilution (45 min) with a polymer detection system (45 min). I'm not sure why it works on one platform but not another. Best of luck, Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003 at umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Thu, Aug 3, 2017 at 8:47 PM, Tony Henwood (SCHN) via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Paula, > > We use Adenovirus antibody from BioSB (Diagnostic Technology) > Cat Number BSB 5040 > Clone 20/11 and 2/6 > Isotype IgG1/K > Dilution 1:200 with citrate retrieval on the Bond 3 > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Friday, 4 August 2017 8:50 AM > To: HistoNet > Subject: [Histonet] Adenovirus antibody? > > Hello Listers, > > I am listing to starboard on this one. We are using the Ventana Ultra > staining platform and have had a bit of trouble with our Adenovirus > stains. We have used Abcam and Millipore to no avail. > > Do you have suggestions about another vendor to try? We are a clinical > lab so we only stain human tissue. IVD is preferred but ASR and RUO are OK > too. > > Any suggestions are gratefully accepted. > > Thank you in advance. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 <#> > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain > confidential information. If you are not the intended recipient, please > delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and > are not necessarily the views of NSW Health or any of its entities. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From travers.3 at osu.edu Fri Aug 4 09:53:29 2017 From: travers.3 at osu.edu (Travers, Susan) Date: Fri, 4 Aug 2017 14:53:29 +0000 Subject: [Histonet] biobond experience? Message-ID: Hi all, Just wondering if anyone has had any experience using biobond as an adhesive for coating slides. We have used a chrome-alum/gelatin coating for years with formalin-fixed, frozen brain sections and it works pretty well. However, with certain stains (e.g., black-gold) we continue to have periodic problems with sections loosening from the slide. Wondering if Biobond might be an improvement? Thanks for any insights ST Susan Travers Professor, Biosciences Division of Biosciences, College of Dentistry The Ohio State University 305 West 12th Avenue Columbus, Ohio 43210 travers.3 at osu.edu (614)361-0800 (cell) (614)292-6366 (work) From cafechague at yahoo.com Fri Aug 4 12:37:31 2017 From: cafechague at yahoo.com (cafechague) Date: Fri, 04 Aug 2017 10:37:31 -0700 Subject: [Histonet] new dako omnis machine vs current dako autostainer link 48 Message-ID: <285583.59584.bm@smtp201.mail.ne1.yahoo.com> hi all!I'm validating our dako omnis. it is a brand new platform. ?and I'm aware that ten positive and negative controls for antibody validations are needed for cap regulations.we are currently using the older dako stainerlink48 with all our antibodies already validated, but plan on moving it to a different location. am i to perform the same protocol for both new and old stainers? or can i just validate 5 positive and negative for the autostainerlink48, instead of having to validate 10.any feedback is appreciated!-cae Sent from my Verizon, Samsung Galaxy smartphone From rfisher0126 at msn.com Fri Aug 4 16:06:37 2017 From: rfisher0126 at msn.com (Renee Fisher) Date: Fri, 4 Aug 2017 21:06:37 +0000 Subject: [Histonet] PIN4 Message-ID: Need help the PIN4 has suddenly stop working we use P63+CK5+CK14 from Bio Care Medical and P504S from Dako. We double stain on the Leica Bond with the Polymer refine Red and Polymer Refine detection. This stain has worked beautifully in the past. Any suggestions? Thank you From houv2 at uw.edu Mon Aug 7 20:08:58 2017 From: houv2 at uw.edu (Vivian Hou) Date: Mon, 7 Aug 2017 18:08:58 -0700 Subject: [Histonet] Examples of Movat on arteries? Message-ID: Dear all, I am learning the Movat Pentachrome stain currently (American Mastertech kit) and trying to stain coronary arteries. I have looked through literature examples but have been unable to locate higher resolution examples of arteries. I would greatly appreciate any example images from experienced histotechs on what the final stain should look like on arteries. Any comments and tips would also be appreciated! Cheers and thank you, Vivian -- ----------------------------------------------------------------------------------------- V Hou Bioengineering | Human Photonics Laboratory e: houv2 at uw.edu | p: 206-221-3813 From edmartin26 at gmail.com Tue Aug 8 01:40:18 2017 From: edmartin26 at gmail.com (Eddie Martin) Date: Tue, 8 Aug 2017 02:40:18 -0400 Subject: [Histonet] PIN4 not working Message-ID: <3F1D53C9-DF56-48AA-AF66-6209ED0A16A5@gmail.com> Hi Kim, I was wondering if you could be more descriptive as to what is going wrong with your PIN4 stain? It's difficult to give a suggestion when we don't have any clue as to what your lab is visualizing. Is it the DAB? Is it the AP Fast Red chromagen? Is it reduced staining or no staining? Kind regards, Eddie Martin, HTL, HT(ASCP), QIHC(ASCP) IHC Histotechnologist III Joint Pathology Center (JPC) Walter Reed National Military Medical Center Bethesda, MD > On Aug 4, 2017, at 5:06 PM, Renee Fisher wrote: > > Need help the PIN4 has suddenly stop working we use P63+CK5+CK14 from Bio Care Medical and P504S from Dako. We double stain on the Leica Bond with the Polymer refine Red and Polymer Refine detection. This stain has worked beautifully in the past. Any suggestions? > > > > Thank you > > From criley at dpspa.com Tue Aug 8 05:45:22 2017 From: criley at dpspa.com (Charles Riley) Date: Tue, 8 Aug 2017 06:45:22 -0400 Subject: [Histonet] H&E Protocols Message-ID: Does anyone use a different staining protocol for their cell blocks from their normal H&E? Our pathologists are all the sudden complaining that the cell blocks are consistently staining lighter than the other specimens. If you do stain differently, in what steps do you modify your protocol and how do you modify it? As always any responses are greatly appreciated -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From relia1 at earthlink.net Tue Aug 8 08:40:11 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 8 Aug 2017 09:40:11 -0400 Subject: [Histonet] Exciting NEW Opportunities in California - Run your own brand new lab and MORE!! Message-ID: <077a01d3104b$dbf207e0$93d617a0$@earthlink.net> Hi Histonetters, How are you? I hope you had a great weekend and are looking forward to a fantastic week!! I am presently on searches for some of my best clients in California that are in need of histology professionals. Can you help? **Most of these positions are RELIA EXCLUSIVES** My clients offer excellent compensation, benefits, in some cases relocation assistance and all of these environments are great to work in. These are permanent full time positions in busy growing labs. Here are my most EXCITING OPPORTUNITIES: ? Ventura, CA - Run your own brand new lab!! ? Pomona, CA ? Run your own brand new lab!! ? San Diego, CA- CLIA qual to Gross ? New Lab ? Irvine, CA - Histotechnician ? AWESOME PLACE TO WORK! ? Irvine, CA - Lead Histology Tech ? Irvine, CA - Histology Lab Manager ? Modesto, CA ? Histology Supervisor - Brand New Position!! The help I need is do you know anyone that might be interested in hearing about any of these opportunities? If so could you please forward my e-mail to them? If I place someone you refer, you will earn a referral fee!! If you are interested in any of these positions please call me ASAP at 866-607-3542 or on my cell/text at 407-353-5070 or e-mail me at relia1 at earthlink.net Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Nancy_Schmitt at pa-ucl.com Tue Aug 8 10:00:32 2017 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Tue, 8 Aug 2017 15:00:32 +0000 Subject: [Histonet] PPE and countertop cleaning Message-ID: <574c591588484bf78ff25d5e80aa91ae@mercury.wad.pa-ucl.com> Good Morning! We are circling again on these two hot topic items! Please share what you are using for PPE for histology processes and do you also wear for embedding/cutting? Countertop cleaning? We went to a purchases wipe and it is eating away the countertops:( Would you please share what you are using and what your process is for countertop cleaning? We are CAP inspected. Thank you much for your input Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From c.tague at Pathologyarts.com Tue Aug 8 11:14:04 2017 From: c.tague at Pathologyarts.com (Curt) Date: Tue, 8 Aug 2017 16:14:04 +0000 Subject: [Histonet] BRCA-1 validation Message-ID: <9C8F910F72893643B3C3793C3D67132B6812EED6@PATHOLOGYSERVER.pathologyarts.local> Just looking for some recommendations on tissue for validating BRCA-1, IHC... before I get to the pathologists... any recommendations. What I'm reading so far is ovarian carcinoma (serous) and normal breast, is that what you all have used and/or recommend? Thanks, Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. From SJMcCabe at phhealthcare.org Tue Aug 8 11:17:49 2017 From: SJMcCabe at phhealthcare.org (McCabe, Sara) Date: Tue, 8 Aug 2017 16:17:49 +0000 Subject: [Histonet] HSV 1/11 Message-ID: <1502209069781.64234@phhealthcare.org> Does anyone have any positive control blocks for HSV? Or for those that are buying their control material any suggestions? Thank you in advance! Sara J. McCabe, HT(ASCP)CM Histology Supervisor Penn Highlands DuBois 100 Hospital Avenue DuBois, PA 15801 814-375-7514 Telephone-Office 814-375-3264 Telephone-Histology Lab 814-375-3784 Fax sjmccabe at phhealthcare.org www.phhealthcare.org [PHH ESig Logo 150dpi-01] This email and any attached files are sensitive in nature and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of Penn Highlands Healthcare or its affiliates.. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. From nto at stowers.org Tue Aug 8 14:36:11 2017 From: nto at stowers.org (Thomas, Nancy) Date: Tue, 8 Aug 2017 19:36:11 +0000 Subject: [Histonet] Histocryl acrylic system Message-ID: I have been trying different resin kits now that Immuno-bed is no longer available. I am ready to try 'Histocryl' but the instructions are unclear to me. Has anyone had experience with this brand? If so, I would like to ask a few questions. Thank you in advance, Nancy Thomas Senior Lab Manager, Histology Core Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 From CDavis at che-east.org Wed Aug 9 07:34:22 2017 From: CDavis at che-east.org (Cassie P. Davis) Date: Wed, 9 Aug 2017 12:34:22 +0000 Subject: [Histonet] Roche-Ventana Special Stainer In-Reply-To: References: Message-ID: Good morning All, My supervisor asked me to get opinions on the newer stand alone automated special stainer from Roche-Ventana is now offering so I am asking. We currently have the 2009 countertop model. Thank you in advance for taking you time to respond. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, August 8, 2017 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [External] Histonet Digest, Vol 165, Issue 6 Warning: This email originated from the Internet! DO NOT CLICK links if the sender is unknown, and NEVER provide your Password. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Examples of Movat on arteries? (Vivian Hou) 2. PIN4 not working (Eddie Martin) 3. H&E Protocols (Charles Riley) 4. Exciting NEW Opportunities in California - Run your own brand new lab and MORE!! (Pam Barker) 5. PPE and countertop cleaning (Nancy Schmitt) 6. BRCA-1 validation (Curt) 7. HSV 1/11 (McCabe, Sara) ---------------------------------------------------------------------- Message: 1 Date: Mon, 7 Aug 2017 18:08:58 -0700 From: Vivian Hou To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Examples of Movat on arteries? Message-ID: Content-Type: text/plain; charset="UTF-8" Dear all, I am learning the Movat Pentachrome stain currently (American Mastertech kit) and trying to stain coronary arteries. I have looked through literature examples but have been unable to locate higher resolution examples of arteries. I would greatly appreciate any example images from experienced histotechs on what the final stain should look like on arteries. Any comments and tips would also be appreciated! Cheers and thank you, Vivian -- ----------------------------------------------------------------------------------------- V Hou Bioengineering | Human Photonics Laboratory e: houv2 at uw.edu | p: 206-221-3813 ------------------------------ Message: 2 Date: Tue, 8 Aug 2017 02:40:18 -0400 From: Eddie Martin To: Renee Fisher Cc: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] PIN4 not working Message-ID: <3F1D53C9-DF56-48AA-AF66-6209ED0A16A5 at gmail.com> Content-Type: text/plain; charset=us-ascii Hi Kim, I was wondering if you could be more descriptive as to what is going wrong with your PIN4 stain? It's difficult to give a suggestion when we don't have any clue as to what your lab is visualizing. Is it the DAB? Is it the AP Fast Red chromagen? Is it reduced staining or no staining? Kind regards, Eddie Martin, HTL, HT(ASCP), QIHC(ASCP) IHC Histotechnologist III Joint Pathology Center (JPC) Walter Reed National Military Medical Center Bethesda, MD > On Aug 4, 2017, at 5:06 PM, Renee Fisher wrote: > > Need help the PIN4 has suddenly stop working we use P63+CK5+CK14 from Bio Care Medical and P504S from Dako. We double stain on the Leica Bond with the Polymer refine Red and Polymer Refine detection. This stain has worked beautifully in the past. Any suggestions? > > > > Thank you > > ------------------------------ Message: 3 Date: Tue, 8 Aug 2017 06:45:22 -0400 From: Charles Riley To: Histo List Subject: [Histonet] H&E Protocols Message-ID: Content-Type: text/plain; charset="UTF-8" Does anyone use a different staining protocol for their cell blocks from their normal H&E? Our pathologists are all the sudden complaining that the cell blocks are consistently staining lighter than the other specimens. If you do stain differently, in what steps do you modify your protocol and how do you modify it? As always any responses are greatly appreciated -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Message: 4 Date: Tue, 8 Aug 2017 09:40:11 -0400 From: "Pam Barker" To: "Histonetters Histonet" Subject: [Histonet] Exciting NEW Opportunities in California - Run your own brand new lab and MORE!! Message-ID: <077a01d3104b$dbf207e0$93d617a0$@earthlink.net> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters, How are you? I hope you had a great weekend and are looking forward to a fantastic week!! I am presently on searches for some of my best clients in California that are in need of histology professionals. Can you help? **Most of these positions are RELIA EXCLUSIVES** My clients offer excellent compensation, benefits, in some cases relocation assistance and all of these environments are great to work in. These are permanent full time positions in busy growing labs. Here are my most EXCITING OPPORTUNITIES: ? Ventura, CA - Run your own brand new lab!! ? Pomona, CA ? Run your own brand new lab!! ? San Diego, CA- CLIA qual to Gross ? New Lab ? Irvine, CA - Histotechnician ? AWESOME PLACE TO WORK! ? Irvine, CA - Lead Histology Tech ? Irvine, CA - Histology Lab Manager ? Modesto, CA ? Histology Supervisor - Brand New Position!! The help I need is do you know anyone that might be interested in hearing about any of these opportunities? If so could you please forward my e-mail to them? If I place someone you refer, you will earn a referral fee!! If you are interested in any of these positions please call me ASAP at 866-607-3542 or on my cell/text at 407-353-5070 or e-mail me at relia1 at earthlink.net Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 5 Date: Tue, 8 Aug 2017 15:00:32 +0000 From: Nancy Schmitt To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] PPE and countertop cleaning Message-ID: <574c591588484bf78ff25d5e80aa91ae at mercury.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" Good Morning! We are circling again on these two hot topic items! Please share what you are using for PPE for histology processes and do you also wear for embedding/cutting? Countertop cleaning? We went to a purchases wipe and it is eating away the countertops:( Would you please share what you are using and what your process is for countertop cleaning? We are CAP inspected. Thank you much for your input Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 6 Date: Tue, 8 Aug 2017 16:14:04 +0000 From: Curt To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] BRCA-1 validation Message-ID: <9C8F910F72893643B3C3793C3D67132B6812EED6 at PATHOLOGYSERVER.pathologyarts.local> Content-Type: text/plain; charset="us-ascii" Just looking for some recommendations on tissue for validating BRCA-1, IHC... before I get to the pathologists... any recommendations. What I'm reading so far is ovarian carcinoma (serous) and normal breast, is that what you all have used and/or recommend? Thanks, Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. ------------------------------ Message: 7 Date: Tue, 8 Aug 2017 16:17:49 +0000 From: "McCabe, Sara" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] HSV 1/11 Message-ID: <1502209069781.64234 at phhealthcare.org> Content-Type: text/plain; charset="iso-8859-1" Does anyone have any positive control blocks for HSV? Or for those that are buying their control material any suggestions? Thank you in advance! Sara J. McCabe, HT(ASCP)CM Histology Supervisor Penn Highlands DuBois 100 Hospital Avenue DuBois, PA 15801 814-375-7514 Telephone-Office 814-375-3264 Telephone-Histology Lab 814-375-3784 Fax sjmccabe at phhealthcare.org www.phhealthcare.org [PHH ESig Logo 150dpi-01] This email and any attached files are sensitive in nature and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of Penn Highlands Healthcare or its affiliates.. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 165, Issue 6 **************************************** Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From angela at lji.org Wed Aug 9 12:51:20 2017 From: angela at lji.org (Angela Lamberth) Date: Wed, 9 Aug 2017 10:51:20 -0700 Subject: [Histonet] Looking to buy a used multistainer Message-ID: Hello everyone! We are a non-profit research institute looking to add a used Leica ST5020 along with the CV5030 coverslipper to our histology core. If you are considering selling yours or know someone who is, I would love to hear from you. -- *Angela Lamberth* | Histology Technician III angela at lji.org *La Jolla Institute for Allergy and Immunology* 858.752.2222 9420 Athena Circle La Jolla, CA 92037 http://www.lji.org From hhuggins at novalabs.co Wed Aug 9 13:01:05 2017 From: hhuggins at novalabs.co (Haley Huggins) Date: Wed, 9 Aug 2017 11:01:05 -0700 Subject: [Histonet] 96 well filter plates Message-ID: I am having some issues with the 96 well filter plates (Millipore brand) and leaking. Not all the wells will leak, just select ones will. Any ideas of why this might be happening or suggestions of other plates that might work better? We vacuum suction out the fluid which why we need the filters. *Haley Huggins, HT (ASCP)cm* *Technical Lab Supervisor* *1050 Las Tablas Rd, Suite 14* *Templeton, CA 93465* *Office: 877-230-1518* *Cell: 303-652-7453* From NMargaryan at luriechildrens.org Wed Aug 9 14:04:06 2017 From: NMargaryan at luriechildrens.org (Margaryan, Naira) Date: Wed, 9 Aug 2017 19:04:06 +0000 Subject: [Histonet] coverslipper CR10030 Message-ID: <34B2EDA118548A4EB35D6E650345BA646B219B88@SV-EX08.childrensmemorial.org> Dear histinetters: We can offer our slightly used DAKO coverslipper CR10030 for purchase. Let us know if you are interested. Thanks, Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) From plucas at biopath.org Thu Aug 10 09:23:59 2017 From: plucas at biopath.org (Paula) Date: Thu, 10 Aug 2017 07:23:59 -0700 Subject: [Histonet] "Dirty" Immunos Message-ID: <002f01d311e4$4fff3f60$effdbe20$@biopath.org> Hello, Hoping we could get a recommendation on helping this issue.. We have a pathologist that complains about "dirty" stains every now and then..not all the time. What could this be/mean? Could it be debris, background staining? We use the Bond III with their ready to use antibodies and their slides for tissue controls. Regarding the "dirty" complaint, could it come from our water baths, covertiles, paraffin, antibodies? I appreciate any feedback to help and I'll try anything. Thank you, Paula From liz at premierlab.com Thu Aug 10 10:57:10 2017 From: liz at premierlab.com (Elizabeth Chlipala) Date: Thu, 10 Aug 2017 09:57:10 -0600 Subject: [Histonet] "Dirty" Immunos In-Reply-To: <002f01d311e4$4fff3f60$effdbe20$@biopath.org> References: <002f01d311e4$4fff3f60$effdbe20$@biopath.org> Message-ID: <14E2C6176416974295479C64A11CB9AE0302D5227226@SBS2K8.premierlab.local> Paula You need to sit down with them and review the slides together, you can't solve a problem without seeing the problem. They need to explain and show you what they consider dirty. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Paula via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, August 10, 2017 8:24 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] "Dirty" Immunos Hello, Hoping we could get a recommendation on helping this issue.. We have a pathologist that complains about "dirty" stains every now and then..not all the time. What could this be/mean? Could it be debris, background staining? We use the Bond III with their ready to use antibodies and their slides for tissue controls. Regarding the "dirty" complaint, could it come from our water baths, covertiles, paraffin, antibodies? I appreciate any feedback to help and I'll try anything. Thank you, Paula _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ From Rosalie.Agrow at piedmont.org Thu Aug 10 13:04:06 2017 From: Rosalie.Agrow at piedmont.org (Rosalie N. Agrow) Date: Thu, 10 Aug 2017 18:04:06 +0000 Subject: [Histonet] SmartWrite cassette printer Message-ID: Looking for users with the Sakura SmartWrite Cassette Printer to compare notes. Please contact me at your convenience. Thank you in advance. Rosalie Agrow, B.S., CT(ASCP) | Manager | Anatomic Pathology Laboratory | 1968 Peachtree Road | 77 Building, 4th Floor | Atlanta, GA 30309 O: 404-605-2142 |F: 404-609-6645 |E: Rosalie.Agrow at piedmont.org If your messaging client supports TLS for secure connections, then this message was sent securely via TLS from Piedmont Healthcare, Inc. This e-mail communication, including any attached files may contain material that is proprietary, privileged, confidential, or otherwise legally exempt from disclosure. This communication is intended solely for the use of the individual or entity to which it is addressed. If you are not the intended recipient or the person responsible for delivering this communication to the intended recipient, you are prohibited from retaining, using, disseminating, forwarding, printing or copying this communication. If you have received this communication in error, please immediately notify the sender via return e-mail or telephone. This email has been scanned and found to be virus free. If this message contains a virus please contact postmaster at piedmont.org. This original email was sent to the internet for delivery at 14:04:30 From melissa at alliedsearchpartners.com Thu Aug 10 14:02:00 2017 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Thu, 10 Aug 2017 19:02:00 +0000 Subject: [Histonet] Histotech Job near Northern NJ/NY State Line Message-ID: Hi Histonetters, Just a quick email to let you know I have a full time permanent Histotech position M-F 6am-2:30pm, near the Northern NJ/New York state lines. Please contact me if you would like more details. This does require a New York Clinical Lab or Histology License. Looking forward to seeing the NSH histonetters. We will be exhibiting at booth 632. Come by! Melissa Owens President, Laboratory Staffing Direct Line: 407.413.9117 Toll Free: 888.388.7571 ext. 102 F: 888.388.7572 From mashalsmom at gmail.com Thu Aug 10 16:41:59 2017 From: mashalsmom at gmail.com (Emily Horst) Date: Thu, 10 Aug 2017 21:41:59 +0000 Subject: [Histonet] IHC Message-ID: Hi everyone, We send our IHCs to an outside lab. I send them the unstained slides. I cut them at 4 microns and put them on charged slides. We are having a lot of issues with the receiving lab saying the tissue is falling off. Their supervisor says the sections are too thick (remember 4 microns) and my pathologist says they are not too thick. Any suggestions for resolving this issue with the receiving lab? Thanks! -- Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio Kettering, Ohio 937.297.0028 From Timothy.Morken at ucsf.edu Thu Aug 10 16:55:42 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 10 Aug 2017 21:55:42 +0000 Subject: [Histonet] IHC In-Reply-To: References: Message-ID: Emily, ? Are you ensuring excess water is not under the section that would prevent adherence to the slide? Are you using slides recommended by the outside lab? Do they recommend a procedure for drying the slides before sending? Just a starting point... Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Emily Horst via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, August 10, 2017 2:42 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC Hi everyone, We send our IHCs to an outside lab. I send them the unstained slides. I cut them at 4 microns and put them on charged slides. We are having a lot of issues with the receiving lab saying the tissue is falling off. Their supervisor says the sections are too thick (remember 4 microns) and my pathologist says they are not too thick. Any suggestions for resolving this issue with the receiving lab? Thanks! -- Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio Kettering, Ohio 937.297.0028 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mashalsmom at gmail.com Thu Aug 10 17:17:41 2017 From: mashalsmom at gmail.com (Emily Horst) Date: Thu, 10 Aug 2017 22:17:41 +0000 Subject: [Histonet] IHC In-Reply-To: References: Message-ID: Thanks everyone for all your suggestions! You've been very helpful! On Thu, Aug 10, 2017 at 6:16 PM Cooper, Brian wrote: > See if you can get a sample of slides from Thermo Fisher. We use the > Superfrost Plus slides, and tissue loss just isn?t one of the problems we > ever have here. And like Tim Morken has said in his response, ensure > there?s no trapped water under the section. > > > > > https://www.fishersci.com/shop/products/shandon-colorfrost-plus-slides/6776214?searchHijack=true&searchTerm=6776214&searchType=RAPID&matchedCatNo=6776214CS > > > > Thanks, > > > > Brian > > > > *From:* Emily Horst [mailto:mashalsmom at gmail.com] > *Sent:* Thursday, August 10, 2017 2:54 PM > > > *To:* Cooper, Brian > *Subject:* Re: [Histonet] IHC (EXTERNAL EMAIL) > > > > They are ones that were prepackaged and sent to us from Dianon (Dianon is > not the lab I'm talking about). I have no idea what/who the slides are. I > just ordered new charged slides from Mercedes Medical and all my invoice > says is 45 degree, white. I haven't received them yet so I don't have a box > with info on it. > > > > It's a problem we've been having for awhile. > > > > On Thu, Aug 10, 2017 at 5:49 PM Cooper, Brian > wrote: > > So I worked at a reference lab for yeeeeeeeeeears and years and years, and > I can tell you that not all charged slides are created equally. What type > of slides are you using? > > > > Thanks, > > > > Brian > > > > *From:* Emily Horst [mailto:mashalsmom at gmail.com] > *Sent:* Thursday, August 10, 2017 2:47 PM > *To:* Cooper, Brian > *Subject:* Re: [Histonet] IHC (EXTERNAL EMAIL) > > > > No I am not. I air dry them and then package them up. > > > > On Thu, Aug 10, 2017 at 5:46 PM Cooper, Brian > wrote: > > Are you baking the slides before sending them out? How long, and what > temperature? > > Thanks, > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 Pager: 213-209-0184 > bcooper at chla.usc.edu > > -----Original Message----- > From: Emily Horst via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, August 10, 2017 2:42 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] IHC (EXTERNAL EMAIL) > > Hi everyone, > > We send our IHCs to an outside lab. I send them the unstained slides. I > cut them at 4 microns and put them on charged slides. We are having a lot > of issues with the receiving lab saying the tissue is falling off. Their > supervisor says the sections are too thick (remember 4 microns) and my > pathologist says they are not too thick. Any suggestions for resolving this > issue with the receiving lab? > > Thanks! > -- > Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio > Kettering, Ohio 937.297.0028 _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, > please > contact the sender by reply e-mail and destroy all copies of this original > message. > > -- > > Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio > Kettering, Ohio 937.297.0028 > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, > please > contact the sender by reply e-mail and destroy all copies of this original > message. > > -- > > Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio > Kettering, Ohio 937.297.0028 > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, > please > contact the sender by reply e-mail and destroy all copies of this original > message. > -- Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio Kettering, Ohio 937.297.0028 From eddessa at emory.edu Fri Aug 11 09:33:07 2017 From: eddessa at emory.edu (Dessasau III, Evan) Date: Fri, 11 Aug 2017 14:33:07 +0000 Subject: [Histonet] IHC In-Reply-To: References: Message-ID: Hi Emily , we do the same thing. It could be the slides but I think it has more to do with how you treat the sections after mounting. The dry time and heat time. I also think it has more to do with technique in the IHC lab. Brains, decal, and fatty tissues get more dry time. All sections get 1 hr. heat at 60C . if they dry overnight 15min heat seems to be fine for us. E-van -----Original Message----- From: Emily Horst via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, August 10, 2017 5:42 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC Hi everyone, We send our IHCs to an outside lab. I send them the unstained slides. I cut them at 4 microns and put them on charged slides. We are having a lot of issues with the receiving lab saying the tissue is falling off. Their supervisor says the sections are too thick (remember 4 microns) and my pathologist says they are not too thick. Any suggestions for resolving this issue with the receiving lab? Thanks! -- Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio Kettering, Ohio 937.297.0028 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From eddessa at emory.edu Fri Aug 11 09:58:26 2017 From: eddessa at emory.edu (Dessasau III, Evan) Date: Fri, 11 Aug 2017 14:58:26 +0000 Subject: [Histonet] IHC In-Reply-To: References: Message-ID: Hi again Ms. Horst, For some reason I did not see all the replies from Mr. Cooper. But wait before your order the Superfrost slides. If you are not familiar with them they are good as Mr. Cooper says but they can be pricey. We use a great guy at PathSUPPLY, who is cc'd here, that can get you a comparable (if not the same) slide at a lower price point. Also, we use slides packaged for PathSupply that are a good price point and excellent quality esp. the Bright White slide is comparable to the Superfrost but at a lower price point. Also, sorry I would suggest you heat them before you send them and communicate with them like someone else said. I always ask how they want them treated before I send the sections. E-van -----Original Message----- From: Emily Horst via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, August 10, 2017 6:18 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC Thanks everyone for all your suggestions! You've been very helpful! On Thu, Aug 10, 2017 at 6:16 PM Cooper, Brian wrote: > See if you can get a sample of slides from Thermo Fisher. We use the > Superfrost Plus slides, and tissue loss just isn?t one of the problems > we ever have here. And like Tim Morken has said in his response, > ensure there?s no trapped water under the section. > > > > > https://www.fishersci.com/shop/products/shandon-colorfrost-plus-slides > /6776214?searchHijack=true&searchTerm=6776214&searchType=RAPID&matched > CatNo=6776214CS > > > > Thanks, > > > > Brian > > > > *From:* Emily Horst [mailto:mashalsmom at gmail.com] > *Sent:* Thursday, August 10, 2017 2:54 PM > > > *To:* Cooper, Brian > *Subject:* Re: [Histonet] IHC (EXTERNAL EMAIL) > > > > They are ones that were prepackaged and sent to us from Dianon (Dianon > is not the lab I'm talking about). I have no idea what/who the slides > are. I just ordered new charged slides from Mercedes Medical and all > my invoice says is 45 degree, white. I haven't received them yet so I > don't have a box with info on it. > > > > It's a problem we've been having for awhile. > > > > On Thu, Aug 10, 2017 at 5:49 PM Cooper, Brian > wrote: > > So I worked at a reference lab for yeeeeeeeeeears and years and years, > and I can tell you that not all charged slides are created equally. > What type of slides are you using? > > > > Thanks, > > > > Brian > > > > *From:* Emily Horst [mailto:mashalsmom at gmail.com] > *Sent:* Thursday, August 10, 2017 2:47 PM > *To:* Cooper, Brian > *Subject:* Re: [Histonet] IHC (EXTERNAL EMAIL) > > > > No I am not. I air dry them and then package them up. > > > > On Thu, Aug 10, 2017 at 5:46 PM Cooper, Brian > wrote: > > Are you baking the slides before sending them out? How long, and what > temperature? > > Thanks, > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of > Pathology and Laboratory Medicine Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 Pager: 213-209-0184 > bcooper at chla.usc.edu > > -----Original Message----- > From: Emily Horst via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, August 10, 2017 2:42 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] IHC (EXTERNAL EMAIL) > > Hi everyone, > > We send our IHCs to an outside lab. I send them the unstained slides. > I cut them at 4 microns and put them on charged slides. We are having > a lot of issues with the receiving lab saying the tissue is falling > off. Their supervisor says the sections are too thick (remember 4 > microns) and my pathologist says they are not too thick. Any > suggestions for resolving this issue with the receiving lab? > > Thanks! > -- > Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio > Kettering, Ohio 937.297.0028 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may > contain confidential or legally privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by reply > e-mail and destroy all copies of this original message. > > -- > > Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio > Kettering, Ohio 937.297.0028 > > CONFIDENTIALITY NOTICE: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may > contain confidential or legally privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by reply > e-mail and destroy all copies of this original message. > > -- > > Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio > Kettering, Ohio 937.297.0028 > > CONFIDENTIALITY NOTICE: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may > contain confidential or legally privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by reply > e-mail and destroy all copies of this original message. > -- Emily Horst, HT (ASCP) Office manager Interventional Pathology of Ohio Kettering, Ohio 937.297.0028 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From james.fortune at unitypoint.org Fri Aug 11 11:11:46 2017 From: james.fortune at unitypoint.org (Fortune, James A.) Date: Fri, 11 Aug 2017 16:11:46 +0000 Subject: [Histonet] VIP 5 VS. VIP 6 Message-ID: Hey Histonet, We are currently looking into purchasing a refurbished backup processor. We recently had issues with both our processors causing patient care delays. Currently we have 2 VIP 6's and one thermos STP 420 AKA POS/Washing machine. The stp 420 is no longer in use because it had spun multiple patient biopsies out of the cassette, one was lost and one was found stuck to another cassette and had to be sent for DNA comparison. We thought about putting it back in service as a backup but the manufacturer requires two PM's a year @ $5,500 per PM, so we are looking at other options. We have received a quote for a refurbished VIP 5 And VIP 6. The VIP 5 is about half the cost of the 6. Does anyone out there know main differences, which one is better? Any info would be greatly appreciated. James (Andy) Fortune Histology Tech Specialist Meriter Health Services Madison, WI 53715 This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. sections 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Karla.Sendelbach at thedacare.org Fri Aug 11 13:50:21 2017 From: Karla.Sendelbach at thedacare.org (Karla Sendelbach) Date: Fri, 11 Aug 2017 18:50:21 +0000 Subject: [Histonet] Histology Supervisor Position ThedaCare Laboratory Appleton WI Message-ID: ThedaCare Laboratory is searching for a full time histology/anatomic pathology supervisor. If you are interested in living in the Fox Valley area of Wisconsin, please contact Betsy West . Betsy.west at thedacare.org Karla Sendelbach-Elizondo, MD Executive Medical Director ThedaCare Laboratory 1818 N Meade Street Appleton, WI 54911 Tel 920.735.7653 or 920.729.2700 Karla.sendelbach at thedacare.org www.thedacare.org From atempleton at tvmdl.tamu.edu Fri Aug 11 14:42:26 2017 From: atempleton at tvmdl.tamu.edu (Alexis Templeton) Date: Fri, 11 Aug 2017 19:42:26 +0000 Subject: [Histonet] Digital Pathology & Coverslipping Message-ID: Hi All! My lab is considering moving up in the world of technology. The goal is to start scanning slides for pathologists to read digitally. We are a relatively high throughput lab and I'm trying to figure out what we need in terms of an automatic coverslipper to avoid drying time. We currently still coverslip by hand and I'm assuming there would be too much wet glue to place the slides directly in a scanner. Tips and recommendations, please! Alexis Templeton, HT (ASCP)CM Diagnostic Laboratory Supervisor Histopathology Texas A&M Veterinary Medical Diagnostic Laboratory P.O. Drawer 3040 | College Station, TX 77841-3040 p: (979) 845-3414 | f: (979) 845-1794 atempleton at tvmdl.tamu.edu http://tvmdl.tamu.edu We Moved! Effective February 27, 2017 our physical (shipping) address is 483 Agronomy Road, College Station, TX 77840. Our billing address remains at PO Drawer 3040, College Station, Texas 77841 ________ **The contents of this email do not necessarily represent the views or policies of TVMDL. This email is intended for the recipient only and the information should not be released to third parties. From jaylundgren at gmail.com Fri Aug 11 16:32:56 2017 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 11 Aug 2017 16:32:56 -0500 Subject: [Histonet] Digital Pathology & Coverslipping In-Reply-To: References: Message-ID: Well, film is the other choice. http://www.sakura.eu/Our-products/item/10/-Coverslipping/82/Tissue-Tek-Film-Coverslipper Agronomy Road, huh, gotta love the Aggies. Hullaballoo Caneck Caneck! Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Aug 11, 2017 at 2:42 PM, Alexis Templeton via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi All! > > My lab is considering moving up in the world of technology. The goal is > to start scanning slides for pathologists to read digitally. We are a > relatively high throughput lab and I'm trying to figure out what we need in > terms of an automatic coverslipper to avoid drying time. We currently > still coverslip by hand and I'm assuming there would be too much wet glue > to place the slides directly in a scanner. Tips and recommendations, > please! > > Alexis Templeton, HT (ASCP)CM > Diagnostic Laboratory Supervisor > Histopathology > Texas A&M Veterinary Medical Diagnostic Laboratory > P.O. Drawer 3040 | College Station, TX 77841-3040 > p: (979) 845-3414 | f: (979) 845-1794 > atempleton at tvmdl.tamu.edu > http://tvmdl.tamu.edu > > We Moved! Effective February 27, 2017 our physical (shipping) address is > 483 Agronomy Road, College Station, TX 77840. Our billing address remains > at PO Drawer 3040, College Station, Texas 77841 > ________ > **The contents of this email do not necessarily represent the views or > policies of TVMDL. This email is intended for the recipient only and the > information should not be released to third parties. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From patpxs at gmail.com Fri Aug 11 17:56:54 2017 From: patpxs at gmail.com (P Sicurello) Date: Fri, 11 Aug 2017 15:56:54 -0700 Subject: [Histonet] VIP 5 VS. VIP 6 In-Reply-To: References: Message-ID: Hi Andy, I prefer the VIP6. It is easier to program, can have more programs and is slightly easier to use than the VIP5. The VIP6 is more computerized than the VIP5 and can generate reports such as error logs, tissue processing runs (to name a few) that you can put on a flash drive for further analysis. The VIP6 also has bulk tanks (we use them for the alcohol and xylene) that can be used to fill up those reagents if they run low during processing. It also has drain and intake ports that can be used to drain the reagent containers with minimal fumes. It can also drain and rotate the paraffin tanks. The VIP6 has a touch screen monitor, the VIP5 does not. If you just need a workhorse processor, the VIP5 should work just fine. I would call Sakura-Finetek and ask what the parts availability is like for the VIP5. They are older models and you never know when parts might become unavailable. My two cents. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Fri, Aug 11, 2017 at 9:11 AM, Fortune, James A. via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hey Histonet, > > We are currently looking into purchasing a refurbished backup processor. > We recently had issues with both our processors causing patient care > delays. Currently we have 2 VIP 6's and one thermos STP 420 AKA POS/Washing > machine. The stp 420 is no longer in use because it had spun multiple > patient biopsies out of the cassette, one was lost and one was found stuck > to another cassette and had to be sent for DNA comparison. We thought > about putting it back in service as a backup but the manufacturer requires > two PM's a year @ $5,500 per PM, so we are looking at other options. We > have received a quote for a refurbished VIP 5 And VIP 6. The VIP 5 is > about half the cost of the 6. Does anyone out there know main differences, > which one is better? Any info would be greatly appreciated. > > James (Andy) Fortune > Histology Tech Specialist > Meriter Health Services > Madison, WI 53715 > This message and accompanying documents are covered by the Electronic > Communications Privacy Act, 18 U.S.C. sections 2510-2521, and contain > information intended for the specified individual(s) only. This information > is confidential. If you are not the intended recipient or an agent > responsible for delivering it to the intended recipient, you are hereby > notified that you have received this document in error and that any review, > dissemination, copying, or the taking of any action based on the contents > of this information is strictly prohibited. If you have received this > communication in error, please notify us immediately by e-mail, and delete > the original message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From patpxs at gmail.com Fri Aug 11 18:04:45 2017 From: patpxs at gmail.com (P Sicurello) Date: Fri, 11 Aug 2017 16:04:45 -0700 Subject: [Histonet] Roche-Ventana Special Stainer In-Reply-To: References: Message-ID: HI Cassie, We have a 2014 Ventana Benchmark Special Stainer and it works great. As much as we use it (and we have been told by Ventana that we are pushing it to its upper limits) and we use it a lot, it still works with little to no problems. The stains are consistent, the silver stains do not vary and the rest look lovely too. Stay away from the Gram and EVG, they don't work. If you have the space for it to stay in one piece that would be nice. We didn't have the space, but we did have a countertop with a knee hole. I separated the top from the bottom (it comes apart and doesn't break it), had holes drilled into the countertop for the hoses, placed the stain unit on top and the reagent portion underneath the countertop and that works well too. I'm sure Ventana would not recommend that everyone do that, but it was our only choice. If you can get one, you should be very happy with it. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Wed, Aug 9, 2017 at 5:34 AM, Cassie P. Davis via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good morning All, > > My supervisor asked me to get opinions on the newer stand alone > automated special stainer from Roche-Ventana is now offering so I am > asking. We currently have the 2009 countertop model. Thank you in advance > for taking you time to respond. > > Cassandra Davis > Histology Technician > AP Laboratory > 302-575-8095 > Email: CDavis at che-east.org > > > > > ________________________________ > From: histonet-request at lists.utsouthwestern.edu utsouthwestern.edu> > Sent: Tuesday, August 8, 2017 1:00 PM > To: histonet at lists.utsouthwestern.edu > Subject: [External] Histonet Digest, Vol 165, Issue 6 > > Warning: This email originated from the Internet! > > DO NOT CLICK links if the sender is unknown, and NEVER provide your > Password. > > > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Examples of Movat on arteries? (Vivian Hou) > 2. PIN4 not working (Eddie Martin) > 3. H&E Protocols (Charles Riley) > 4. Exciting NEW Opportunities in California - Run your own brand > new lab and MORE!! (Pam Barker) > 5. PPE and countertop cleaning (Nancy Schmitt) > 6. BRCA-1 validation (Curt) > 7. HSV 1/11 (McCabe, Sara) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 7 Aug 2017 18:08:58 -0700 > From: Vivian Hou > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Examples of Movat on arteries? > Message-ID: > gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Dear all, > > I am learning the Movat Pentachrome stain currently (American Mastertech > kit) and trying to stain coronary arteries. I have looked through > literature examples but have been unable to locate higher resolution > examples of arteries. > > I would greatly appreciate any example images from experienced histotechs > on what the final stain should look like on arteries. Any comments and tips > would also be appreciated! > > Cheers and thank you, > Vivian > > -- > ------------------------------------------------------------ > ----------------------------- > V Hou > Bioengineering | Human Photonics Laboratory > e: houv2 at uw.edu | p: 206-221-3813 > > > ------------------------------ > > Message: 2 > Date: Tue, 8 Aug 2017 02:40:18 -0400 > From: Eddie Martin > To: Renee Fisher > Cc: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] PIN4 not working > Message-ID: <3F1D53C9-DF56-48AA-AF66-6209ED0A16A5 at gmail.com> > Content-Type: text/plain; charset=us-ascii > > > Hi Kim, > > I was wondering if you could be more descriptive as to what is going wrong > with your PIN4 stain? > > It's difficult to give a suggestion when we don't have any clue as to what > your lab is visualizing. > > Is it the DAB? > Is it the AP Fast Red chromagen? > Is it reduced staining or no staining? > > Kind regards, > > Eddie Martin, HTL, HT(ASCP), QIHC(ASCP) > IHC Histotechnologist III > Joint Pathology Center (JPC) > Walter Reed National Military Medical Center > Bethesda, MD > > > > > > On Aug 4, 2017, at 5:06 PM, Renee Fisher wrote: > > > > Need help the PIN4 has suddenly stop working we use P63+CK5+CK14 from > Bio Care Medical and P504S from Dako. We double stain on the Leica Bond > with the Polymer refine Red and Polymer Refine detection. This stain has > worked beautifully in the past. Any suggestions? > > > > > > > > Thank you > > > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 8 Aug 2017 06:45:22 -0400 > From: Charles Riley > To: Histo List > Subject: [Histonet] H&E Protocols > Message-ID: > fRKQ_LA at mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Does anyone use a different staining protocol for their cell blocks from > their normal H&E? > > Our pathologists are all the sudden complaining that the cell blocks are > consistently staining lighter than the other specimens. > > If you do stain differently, in what steps do you modify your protocol and > how do you modify it? > > As always any responses are greatly appreciated > > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > > > ------------------------------ > > Message: 4 > Date: Tue, 8 Aug 2017 09:40:11 -0400 > From: "Pam Barker" > To: "Histonetters Histonet" > Subject: [Histonet] Exciting NEW Opportunities in California - Run > your own brand new lab and MORE!! > Message-ID: <077a01d3104b$dbf207e0$93d617a0$@earthlink.net> > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonetters, > How are you? I hope you had a great weekend and are looking forward to a > fantastic week!! > I am presently on searches for some of my best clients in California that > are in need of histology professionals. > > Can you help? > > **Most of these positions are RELIA EXCLUSIVES** > > My clients offer excellent compensation, benefits, in some cases relocation > assistance and all of these environments are great to work in. > These are permanent full time positions in busy growing labs. > > Here are my most EXCITING OPPORTUNITIES: > ? Ventura, CA - Run your own brand new lab!! > ? Pomona, CA ? Run your own brand new lab!! > ? San Diego, CA- CLIA qual to Gross ? New Lab > ? Irvine, CA - Histotechnician ? AWESOME PLACE TO WORK! > ? Irvine, CA - Lead Histology Tech > ? Irvine, CA - Histology Lab Manager > ? Modesto, CA ? Histology Supervisor - Brand New Position!! > > The help I need is do you know anyone that might be interested in hearing > about any of these opportunities? If so could you please forward my e-mail > to them? > > If I place someone you refer, you will earn a referral fee!! > > If you are interested in any of these positions please call me ASAP at > 866-607-3542 or on my cell/text at 407-353-5070 or e-mail me at > relia1 at earthlink.net > > > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > ?Pam M. Barker > ? > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell:???? (407)353-5070 > FAX:???? (407)678-2788 > E-mail: relia1 at earthlink.net > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > www.facebook.com/PamBarkerRELIA > www.linkedin.com/in/reliasolutions linkedin.com/in/reliasolutions> > www.twitter.com/pamatrelia > > > > > > ------------------------------ > > Message: 5 > Date: Tue, 8 Aug 2017 15:00:32 +0000 > From: Nancy Schmitt > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: [Histonet] PPE and countertop cleaning > Message-ID: <574c591588484bf78ff25d5e80aa91ae at mercury.wad.pa-ucl.com> > Content-Type: text/plain; charset="us-ascii" > > Good Morning! > We are circling again on these two hot topic items! > Please share what you are using for PPE for histology processes and do you > also wear for embedding/cutting? > Countertop cleaning? We went to a purchases wipe and it is eating away > the countertops:( Would you please share what you are using and what your > process is for countertop cleaning? > We are CAP inspected. > > Thank you much for your input > > Nancy Schmitt MLT, HT(ASCP) > Pathology Support Services Manager > > > > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > > ------------------------------ > > Message: 6 > Date: Tue, 8 Aug 2017 16:14:04 +0000 > From: Curt > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] BRCA-1 validation > Message-ID: > <9C8F910F72893643B3C3793C3D67132B6812EED6 at PATHOLOGYSERVER. > pathologyarts.local> > > Content-Type: text/plain; charset="us-ascii" > > Just looking for some recommendations on tissue for validating BRCA-1, > IHC... before I get to the pathologists... any recommendations. What I'm > reading so far is ovarian carcinoma (serous) and normal breast, is that > what you all have used and/or recommend? > > Thanks, > Curt > > > > > CONFIDENTIALITY NOTE: The information transmitted, including attachments, > is intended only for the person(s) or entity to which it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of, or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this in error, please > contact the sender and destroy any copies of this information. > > > ------------------------------ > > Message: 7 > Date: Tue, 8 Aug 2017 16:17:49 +0000 > From: "McCabe, Sara" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] HSV 1/11 > Message-ID: <1502209069781.64234 at phhealthcare.org> > Content-Type: text/plain; charset="iso-8859-1" > > Does anyone have any positive control blocks for HSV? Or for those that > are buying their control material any suggestions? > > Thank you in advance! > Sara J. McCabe, HT(ASCP)CM > Histology Supervisor > Penn Highlands DuBois > 100 Hospital Avenue > DuBois, PA 15801 > 814-375-7514 Telephone-Office > 814-375-3264 Telephone-Histology Lab > 814-375-3784 Fax > sjmccabe at phhealthcare.org > www.phhealthcare.org > > [PHH ESig Logo 150dpi-01] > > This email and any attached files are sensitive in nature and intended > solely for the intended recipient(s). If you are not the named recipient > you should not read, distribute, copy or alter this email. Any views or > opinions expressed in this email are those of the author and do not > represent those of Penn Highlands Healthcare or its affiliates.. Warning: > Although precautions have been taken to make sure no viruses are present in > this email, the company cannot accept responsibility for any loss or damage > that arise from the use of this email or attachments. > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 165, Issue 6 > **************************************** > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From hhuggins at novalabs.co Mon Aug 14 10:48:26 2017 From: hhuggins at novalabs.co (Haley Huggins) Date: Mon, 14 Aug 2017 08:48:26 -0700 Subject: [Histonet] Digital Pathology & Coverslipping In-Reply-To: References: Message-ID: Glass or film coverslips are fine, but you have to make sure they are clean, no excess mounting media, or bubbles. The scanners pick up a lot of extra things you don't want scanned. Also, check with your pathologists to see if they have an opinion one way or another about which coverslips they prefer. *Haley Huggins, HT (ASCP)cm* *Technical Lab Supervisor* *1050 Las Tablas Rd, Suite 14* *Templeton, CA 93465* *Office: 877-230-1518* *Cell: 303-652-7453* On Fri, Aug 11, 2017 at 12:42 PM, Alexis Templeton via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi All! > > My lab is considering moving up in the world of technology. The goal is > to start scanning slides for pathologists to read digitally. We are a > relatively high throughput lab and I'm trying to figure out what we need in > terms of an automatic coverslipper to avoid drying time. We currently > still coverslip by hand and I'm assuming there would be too much wet glue > to place the slides directly in a scanner. Tips and recommendations, > please! > > Alexis Templeton, HT (ASCP)CM > Diagnostic Laboratory Supervisor > Histopathology > Texas A&M Veterinary Medical Diagnostic Laboratory > P.O. Drawer 3040 | College Station, TX 77841-3040 > p: (979) 845-3414 | f: (979) 845-1794 > atempleton at tvmdl.tamu.edu > http://tvmdl.tamu.edu > > We Moved! Effective February 27, 2017 our physical (shipping) address is > 483 Agronomy Road, College Station, TX 77840. Our billing address remains > at PO Drawer 3040, College Station, Texas 77841 > ________ > **The contents of this email do not necessarily represent the views or > policies of TVMDL. This email is intended for the recipient only and the > information should not be released to third parties. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sandra.cheasty at wisc.edu Mon Aug 14 11:12:01 2017 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Mon, 14 Aug 2017 16:12:01 +0000 Subject: [Histonet] "Supa Mega Clamp" Message-ID: Hello all, We use the "Supa Mega Clamp" (Ted Pella) for sectioning large tissues in the oversized cassettes. Lately, it seems not to grip as tight as it did when we received it a month or so ago. The literature is little more than photos of how to attach the clamp to our microtomes. Has anyone experienced this? Is there an adjustment that can be made? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From gu.lang at gmx.at Mon Aug 14 12:02:39 2017 From: gu.lang at gmx.at (Gudrun Lang) Date: Mon, 14 Aug 2017 19:02:39 +0200 Subject: [Histonet] Digital Pathology & Coverslipping In-Reply-To: References: Message-ID: <001201d3151f$246589c0$6d309d40$@gmx.at> Hi! We scan our daily HEs for 1,5 year now. We have a Leica glass-coverslipper. The brilliance of the images is very good. With film we had the experience, that the brilliance of digital photos were never of the same quality as with glass. (and had always issues with coming off the film after a year of storage. So I doubt, that scanning older slides is successful.) The drawback of the glass coverslipper is, that you have always to adjust it. One day it is perfect without any bubbles, the other day the medium has changed the consistency and you need more or less of it. When the medium squeezes out a little bit, the scanner may recognise it as tissue. The same happens, when the medium is spared at the edges and air goes in. As solvent we use butyl-acetate, that evaporates very quickly and clean. The slides are dry within minutes. Our workflow is first to organise the cases for delivering, then scan the sorted cases and then deliver the slides to the pathologists. So the slides have enough time to dry and we can check the quality of coverslipping and quality of the slide-barcode. bye Gudrun -----Urspr?ngliche Nachricht----- Von: Haley Huggins via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Montag, 14. August 2017 17:48 An: Alexis Templeton Cc: histonet at lists.utsouthwestern.edu Betreff: Re: [Histonet] Digital Pathology & Coverslipping Glass or film coverslips are fine, but you have to make sure they are clean, no excess mounting media, or bubbles. The scanners pick up a lot of extra things you don't want scanned. Also, check with your pathologists to see if they have an opinion one way or another about which coverslips they prefer. *Haley Huggins, HT (ASCP)cm* *Technical Lab Supervisor* *1050 Las Tablas Rd, Suite 14* *Templeton, CA 93465* *Office: 877-230-1518* *Cell: 303-652-7453* On Fri, Aug 11, 2017 at 12:42 PM, Alexis Templeton via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi All! > > My lab is considering moving up in the world of technology. The goal > is to start scanning slides for pathologists to read digitally. We > are a relatively high throughput lab and I'm trying to figure out what > we need in terms of an automatic coverslipper to avoid drying time. > We currently still coverslip by hand and I'm assuming there would be > too much wet glue to place the slides directly in a scanner. Tips and > recommendations, please! > > Alexis Templeton, HT (ASCP)CM > Diagnostic Laboratory Supervisor > Histopathology > Texas A&M Veterinary Medical Diagnostic Laboratory P.O. Drawer 3040 | > College Station, TX 77841-3040 > p: (979) 845-3414 | f: (979) 845-1794 > atempleton at tvmdl.tamu.edu > http://tvmdl.tamu.edu > > We Moved! Effective February 27, 2017 our physical (shipping) address > is > 483 Agronomy Road, College Station, TX 77840. Our billing address > remains at PO Drawer 3040, College Station, Texas 77841 ________ > **The contents of this email do not necessarily represent the views or > policies of TVMDL. This email is intended for the recipient only and > the information should not be released to third parties. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Tue Aug 15 13:23:52 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 15 Aug 2017 14:23:52 -0400 Subject: [Histonet] Relia Hot Histology Job Alert - 8-15 - 2017 Exciting New Opportunities as the Summer Flies By!! Message-ID: <1e8501d315f3$a63a4830$f2aed890$@earthlink.net> Hello Histonetters How are you doing today? Is it me or is this Summer FLYING by? I am sending this special alert out because I am ending the summer just like I started it with some of the best job opportunities out there. I am especially excited about these openings because they are with several of my best clients. Why do I refer to them as best clients? Well for a variety of reasons: ? Location, ? The rest of the team, ? The fantastic leadership, ? Excellent benefits, ? Generous relocation ? And most importantly I like a client that has openings due to growth. Here is a list of the positions that I am most excited about: Histology Management/Supervisory Opportunities: ? Stockton, CA Histology Supervisor brand new due to growth! ? Irvine, CA Histology Supervisor (Vet Lab) ? Atlanta, GA Histology Manager (Dermpath) ? Ventura, CA Lead Histotech Brand New Lab! ? Boston, MA Histology Manager Research! Histotechnicians/Histotechnologists Charlotte, NC Histotechnologist IHC exper a plus. San Diego, CA Grossing Histotech CLIA Qualified! Irvine, CA Histotechnician Learn or use your IHC! Ventura, CA Histology Tech Exciting Brand New Lab Arlington, MA Histology Tech Great environment to work in! Chattanooga, TN Grossing Histology Tech CLIA Qualified If you or anyone you know is interested in more details, I can be reached toll free at 866-607-3542 or on my cell at 407-353-5070 or at relia1 at earthlink.net. If you are starting to save up your Christmas money remember, $$$$ I pay a referral fee to you if I place someone that you refer to me. Thanks and have a great day!! Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From thisisann at aol.com Tue Aug 15 13:35:24 2017 From: thisisann at aol.com (Ann Specian) Date: Tue, 15 Aug 2017 14:35:24 -0400 Subject: [Histonet] Fuelgen stain for urine cytology Message-ID: <15de72dcc44-18eb-1dbe8@webprd-a43.mail.aol.com> Does anyone have a staining procedure for the fuelgen stain for urine cytology that they can share Sent from AOL Mobile Mail From gentras at auburn.edu Wed Aug 16 12:05:44 2017 From: gentras at auburn.edu (Atoska Gentry) Date: Wed, 16 Aug 2017 17:05:44 +0000 Subject: [Histonet] blades Message-ID: Hello does anyone have a source for Personna Single-edge stainless steel razor blades? Our former vendor no longer stocks them. Thanks, Atoska From aeck at dh.org Thu Aug 17 05:36:40 2017 From: aeck at dh.org (Eck, Allison) Date: Thu, 17 Aug 2017 10:36:40 +0000 Subject: [Histonet] on call autopsy services Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A973C30E4@DH-MAIL01.dhorg.org> Good morning Histonet, I am not sure if anyone can help me with this question, but I was asked to look into the possibility of finding a pathologist who would be willing to come in and perform our autopsies. We do only few each year so we are not looking to hire someone full time but are exploring the option of performing the autopsies in house. Can anyone point me in a direction to start? Thanks in advance Allison Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT) Lead Tech Histology Doylestown Hospital 595 W State St Doylestown, PA 18901 215-345-2264 aeck at dh.org From bakevictoria at gmail.com Thu Aug 17 05:59:30 2017 From: bakevictoria at gmail.com (Victoria Baker) Date: Thu, 17 Aug 2017 06:59:30 -0400 Subject: [Histonet] on call autopsy services In-Reply-To: <4ED8C96A8F20FC4F883A92E2A0A0D64A973C30E4@DH-MAIL01.dhorg.org> References: <4ED8C96A8F20FC4F883A92E2A0A0D64A973C30E4@DH-MAIL01.dhorg.org> Message-ID: Have you looked at an ad locum pathologist? Or a retired pathologist that would be willing to work as a per diem? The county coroner might even be willing to get you information or know of a pathologist willing to come in. But do you have a dinner or a Histologist willing to do the assisting? There are also funeral or certified morticians who can do assisting. I apologise for any misspelled words. I haven't had my tea and my phone thinks it's a spelling scholar. Vikki On Aug 17, 2017 6:53 AM, "Eck, Allison via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > Good morning Histonet, > I am not sure if anyone can help me with this question, but I was asked to > look into the possibility of finding a pathologist who would be willing to > come in and perform our autopsies. We do only few each year so we are not > looking to hire someone full time but are exploring the option of > performing the autopsies in house. Can anyone point me in a direction to > start? > > Thanks in advance > Allison > > Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT) > Lead Tech Histology > Doylestown Hospital > 595 W State St > Doylestown, PA 18901 > 215-345-2264 > aeck at dh.org > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lmarie08 at uga.edu Thu Aug 17 08:29:14 2017 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Thu, 17 Aug 2017 13:29:14 +0000 Subject: [Histonet] overseas jobs Message-ID: Hello all in Histoland, I am just curious: at most histology conferences I've attended, there's been the general consensus that there is a histotech shortage in the US. Does anyone know if there is a shortage anywhere else in the world? I am wondering if being a histotech is advantageous if you are looking for a job overseas :). Hope you all have a great Thursday! Lauren From relia1 at earthlink.net Thu Aug 17 10:35:56 2017 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 17 Aug 2017 11:35:56 -0400 Subject: [Histonet] RELIA Hot Job Alert!! Brand new histology positions in Atlanta, GA - day and evening shifts!! Contact me ASAP! Message-ID: <01ec01d3176e$85e2c8b0$91a85a10$@earthlink.net> Hi Histonetters! I hope you are having a great day. I have some exciting news to share! I just got several brand new histology positions in a private lab located in Atlanta, GA I have day and evening shifts. For more info please call me - Pam Barker toll free 866-607-3542 or on my cell at 407-353-5070 or email me at relia1 at earthlink.net Have a great day!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From CDavis at che-east.org Fri Aug 18 08:38:47 2017 From: CDavis at che-east.org (Cassie P. Davis) Date: Fri, 18 Aug 2017 13:38:47 +0000 Subject: [Histonet] Histology Shortage? Message-ID: <4e0529171f6941ebb58a4be0093f97d5@che-east.org> I am not sure about overseas but the New Castle County does not have a shortage. There is a school in Delaware that gradutes 5-6 students a year. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From jaylundgren at gmail.com Fri Aug 18 14:09:33 2017 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 18 Aug 2017 14:09:33 -0500 Subject: [Histonet] overseas jobs In-Reply-To: References: Message-ID: It's not a matter of whether there is a shortage or not. Most western, developed countries, unlike the US, have strict laws forbidding non-citizens from working, which they zealously enforce. It is virtually impossible for a foreigner to work (legally) in the EU, for example. Canada has different educational and licensing requirements for histotechs, as does Great Britain. (histotechs must have a BS in Great Britain) I know it is a very involved process for Americans to work in Australia. You have to be in certain categories in which they have a shortage. You would have to check with the Australian consulate. I know you actually have to have a job offer in one of these areas before you can apply for a work visa, it's not like you just show up and start looking. Seems like most South American countries need a visa and work permit. I can only imagine the levels of red tape, corruption, and bribery you would have to engage in. Maybe Asia or Africa? I wonder how much a histo job pays in the Congo, where the average annual income is $422.? Singapore and the Gulf States hire a lot of foreign workers, maybe you could look into that. Also Saudi Arabia, but you have to sign a long contract and give up your passport. They treat non-Saudi workers extremely poorly, according to people I've known who worked there. One co-worker told me about his experience where the whole hospital staff was led outside and forced to watch a non-Saudi doctor get publicly flogged. (He had examined a female patient without the requisite female relative in the room.) Good luck! On Thu, Aug 17, 2017 at 8:29 AM, Lauren Sweeney via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello all in Histoland, > > I am just curious: at most histology conferences I've attended, there's > been the general consensus that there is a histotech shortage in the US. > Does anyone know if there is a shortage anywhere else in the world? I am > wondering if being a histotech is advantageous if you are looking for a job > overseas :). > > Hope you all have a great Thursday! > > Lauren > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TNMayer at mdanderson.org Fri Aug 18 14:14:19 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Fri, 18 Aug 2017 19:14:19 +0000 Subject: [Histonet] Histology Shortage Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8839FAB481@D1PWPEXMBX08.mdanderson.edu> In areas where there are schools, usually there is no shortage. Like here in Houston, we have 2 programs so no shortage. In other areas it is challenging to find a tech. Canada does not seem to have a shortage (that I have heard of), but I know that it can be hard for areas in the Caribbean to find techs. I encourage my students to check the job boards frequently and be willing to relocate. I know I would if I were younger and had no husband or kids. Toysha Today's Topics: 1. Histology Shortage? (Cassie P. Davis) ---------------------------------------------------------------------- Message: 1 Date: Fri, 18 Aug 2017 13:38:47 +0000 From: "Cassie P. Davis" To: histonet Subject: [Histonet] Histology Shortage? Message-ID: <4e0529171f6941ebb58a4be0093f97d5 at che-east.org> Content-Type: text/plain; charset="iso-8859-1" I am not sure about overseas but the New Castle County does not have a shortage. There is a school in Delaware that gradutes 5-6 students a year. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From Tom_Wells at bcit.ca Fri Aug 18 14:29:43 2017 From: Tom_Wells at bcit.ca (Tom Wells) Date: Fri, 18 Aug 2017 19:29:43 +0000 Subject: [Histonet] Histology Shortage In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC8839FAB481@D1PWPEXMBX08.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC8839FAB481@D1PWPEXMBX08.mdanderson.edu> Message-ID: Hi, Just to clarify. Part of the reason that there are fewer specific Histotech shortages in Canada is that Histotechnology is part of the general medical laboratory technology training. So any laboratory technologist could take a job as a histotech. Overall, the need for laboratory technologists in Canada is still great. Tom Tom Wells Histology Faculty Medical Laboratory Sciences School of Health Sciences. BCIT P: (604) 412-7594 -----Original Message----- From: Mayer,Toysha N via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, August 18, 2017 12:14 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Histology Shortage In areas where there are schools, usually there is no shortage. Like here in Houston, we have 2 programs so no shortage. In other areas it is challenging to find a tech. Canada does not seem to have a shortage (that I have heard of), but I know that it can be hard for areas in the Caribbean to find techs. I encourage my students to check the job boards frequently and be willing to relocate. I know I would if I were younger and had no husband or kids. Toysha Today's Topics: 1. Histology Shortage? (Cassie P. Davis) ---------------------------------------------------------------------- Message: 1 Date: Fri, 18 Aug 2017 13:38:47 +0000 From: "Cassie P. Davis" To: histonet Subject: [Histonet] Histology Shortage? Message-ID: <4e0529171f6941ebb58a4be0093f97d5 at che-east.org> Content-Type: text/plain; charset="iso-8859-1" I am not sure about overseas but the New Castle County does not have a shortage. There is a school in Delaware that gradutes 5-6 students a year. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alisha at ka-recruiting.com Mon Aug 21 12:42:39 2017 From: alisha at ka-recruiting.com (Alisha Dynan) Date: Mon, 21 Aug 2017 13:42:39 -0400 Subject: [Histonet] Are you looking for a new histology job? Message-ID: Hi Histonet Members, Please check out our current histology jobs at jobs.ka-recruiting.com. Sincerely, *Alisha Dynan, Founder* *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 Office Phone: (617) 746-2742 *(please note this is a new number)* Cell & Text : 508-341-4218 Fax: (617) 507-8009 alisha at ka-recruiting.com Our openings are updated daily at jobs.ka-recruiting.com From GauchV at mail.amc.edu Mon Aug 21 13:09:11 2017 From: GauchV at mail.amc.edu (Gauch, Vicki) Date: Mon, 21 Aug 2017 18:09:11 +0000 Subject: [Histonet] Anti-Roll plates Message-ID: Hi, Does anyone know a source to purchase glass anti roll plates for our Thermo cryostat ? The one we have had for many years was recently broken and the plastic one we purchased is just not working out for us. Any help would be greatly appreciated. Thanks, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From cmconway at usgs.gov Tue Aug 22 09:39:02 2017 From: cmconway at usgs.gov (Conway, Carla) Date: Tue, 22 Aug 2017 07:39:02 -0700 Subject: [Histonet] Dissolving precipitate in formalin? Message-ID: Hello, I have 4 bottles of formalin which contain white flakes of paraformaldehyde precipitate. I tried to dissolve the flakes by heating to ~65 degrees C for 45 min (with stirring). Has anyone been successful in dissolving this type of precipitate? Thanks very much, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway at usgs.gov From Nancy_Schmitt at pa-ucl.com Tue Aug 22 10:51:00 2017 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Tue, 22 Aug 2017 15:51:00 +0000 Subject: [Histonet] ANP.11640 competency for Non-Pathologist Message-ID: <78bbd9580d664a859a2d71a7e4c9f769@mercury.wad.pa-ucl.com> Hello- Would you share what you are doing to show competency for Non-Pathology (HT with high complex) staff that are grossing derms, GI's, etc.? Thank you Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From criley at dpspa.com Tue Aug 22 10:56:55 2017 From: criley at dpspa.com (Charles Riley) Date: Tue, 22 Aug 2017 11:56:55 -0400 Subject: [Histonet] Microwave processing special stains Message-ID: Does anyone use the KOS microwave processor for their special stains? If so did you purchase the histo-module from them or have you validated another reagent jar? Trying to keep costs low at the moment so management wants to know if there is any alternative to their modules. Thanks -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From cmconway at usgs.gov Tue Aug 22 11:08:14 2017 From: cmconway at usgs.gov (Conway, Carla) Date: Tue, 22 Aug 2017 09:08:14 -0700 Subject: [Histonet] Fwd: Dissolving precipitate in formalin? In-Reply-To: References: Message-ID: Hi Lauren, Thanks so much for your reply. I've seen the precipitate you refer to, but this looks really different. Many of the flakes are large (5-7 mm) and were at the bottom of the bottle. The bottles were previously unopened and I didn't notice the flakes until preparing fixative for a study this morning. Am wondering if adding NaOH will help (similar to dissolving paraformaldehyde for EM fixative). Thanks again! Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway at usgs.gov ---------- Forwarded message ---------- From: Lauren Sweeney Date: Tue, Aug 22, 2017 at 8:02 AM Subject: RE: [Histonet] Dissolving precipitate in formalin? To: "Conway, Carla" I've always been told that that is the salts flaking out and that you can't dissolve them, usually found accumulating around the spigot or opening of wherever it is poured out, we always just brush it off and if re-using the container, can dissolve after running under very hot tap water. Hope this helps! -----Original Message----- From: Conway, Carla via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 22, 2017 10:39 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Dissolving precipitate in formalin? Hello, I have 4 bottles of formalin which contain white flakes of paraformaldehyde precipitate. I tried to dissolve the flakes by heating to ~65 degrees C for 45 min (with stirring). Has anyone been successful in dissolving this type of precipitate? Thanks very much, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway at usgs.gov _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lindsey.s.fairbourn at aruplab.com Tue Aug 22 11:45:05 2017 From: lindsey.s.fairbourn at aruplab.com (Fairbourn, Lindsey S.) Date: Tue, 22 Aug 2017 16:45:05 +0000 Subject: [Histonet] TMA Molds Message-ID: <311174587A85194FBB1007C9DB5D9AE3016AFC3F98@EXMBX1.aruplab.net> Hello Histonet, My lab is currently struggling with the stability of our TMA molds and are looking for an alternative. Does anyone have any suggestions for good quality TMA molds? Thanks for your help! Lindsey Lindsey Fairbourn, HTL(ASCP) AP Histology Research Technical Supervisor, NY 1950 Circle of Hope Drive, rm N3970 Salt Lake City, Utah 84112-5500 Lindsey.s.fairbourn at aruplab.com Lab: (801)213-4272 ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From mbireir at yahoo.com Tue Aug 22 17:39:16 2017 From: mbireir at yahoo.com (MANAHIL EL BIREIR) Date: Wed, 23 Aug 2017 10:39:16 +1200 Subject: [Histonet] Brain Protocol Message-ID: <43D06FD6-D6EE-4659-934B-AB4AED6BA132@yahoo.com> Hi histo. Would be very appreciated if any share Brain processing Protocol. We have an issue with Brain processing that is really soft and looks un processed. Thanks, Manahil Sent from my iPhone From Albert.Santiago at uphs.upenn.edu Thu Aug 24 10:41:20 2017 From: Albert.Santiago at uphs.upenn.edu (Santiago, Albert) Date: Thu, 24 Aug 2017 15:41:20 +0000 Subject: [Histonet] ELISA assays Message-ID: Greetings my fellow histonetters, Does anyone out there that run ELISA assays use the interpretation results from the package insert or do you establish your own reference ranges? Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market ST. Ste 201 Philadelphia, PA 19104 215-662-6539 - Lab 215-662-6759-office albert.santiago at uphs.upenn.edu From raestask at grics.net Fri Aug 25 13:12:25 2017 From: raestask at grics.net (raestask at grics.net) Date: Fri, 25 Aug 2017 14:12:25 -0400 (EDT) Subject: [Histonet] Short biopsy run Message-ID: <52077325.103169458.1503684745651.JavaMail.root@grics.net> Does anyone have a short biopsy protocol for a VIP 6? ?or VIP 6 AI? If so could you please share it with us? Thank you in advance. Rae Staskiewicz UnityPoint? health Methodist, Peoria IL From sandra.cheasty at wisc.edu Mon Aug 28 10:23:45 2017 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Mon, 28 Aug 2017 15:23:45 +0000 Subject: [Histonet] Cardboard File Drawers for Blocks Message-ID: Hello everyone, Does anyone know of a company that sells the cardboard drawers and drawer dividers for filing blocks, separate from the outer cardboard file that the drawers slide into? We came into possession of 25 outer files with no drawers, which means we need 200 drawers and dividers. Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From jhill at vet.k-state.edu Mon Aug 28 10:30:08 2017 From: jhill at vet.k-state.edu (Jennifer Phinney) Date: Mon, 28 Aug 2017 15:30:08 +0000 Subject: [Histonet] Cut FFPE Slides for ISH Message-ID: Hello Histonet, I am hoping someone can point me in the right direction to find some references for how long FFPE slides intended for ISH can be kept before staining. I have a researcher who swears their slides have to be stained within 24 hours of being cut, which does not sound correct to me. I've used ACD's RNAscope before and their protocol lists the timeframe as 3 months (so this is a bit of a difference). There's a lot of information about fixation times, but I've come up empty handed for cut slides. Thanks everyone! Jennifer Phinney QIHCCM Project Coordinator Kansas State University Veterinary Diagnostic Lab From sandra.cheasty at wisc.edu Mon Aug 28 10:30:13 2017 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Mon, 28 Aug 2017 15:30:13 +0000 Subject: [Histonet] Paraformaldehyde Fixed Tissue Message-ID: Hi all, We are having difficulty sectioning mouse tissue, (brain, spinal cord, liver, and spleen), on paraformaldehyde fixed tissue. Has anyone had issues with paraformaldehyde fixed tissue? They were processed routinely, starting in 10% NBF, with other tissues, and we are cutting them at 3u. Thank you! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From tina.vanmeter at gmail.com Mon Aug 28 11:37:20 2017 From: tina.vanmeter at gmail.com (Tina Van Meter) Date: Mon, 28 Aug 2017 12:37:20 -0400 Subject: [Histonet] Paraformaldehyde Fixed Tissue In-Reply-To: References: Message-ID: Hi Sandra, What are the times for each processing station?? Animal tissue is much leaner, so it's easy to over process and become brittle to section. Best, Tina Van Meter On Mon, Aug 28, 2017 at 11:30 AM, Sandra Cheasty via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all, > We are having difficulty sectioning mouse tissue, (brain, > spinal cord, liver, and spleen), on paraformaldehyde fixed tissue. Has > anyone had issues with paraformaldehyde fixed tissue? They were processed > routinely, starting in 10% NBF, with other tissues, and we are cutting them > at 3u. > Thank you! > Sandy > > Sandra J. Cheasty, HT (ASCP) > Histology & Necropsy Supervisor > UW-Madison, School of Veterinary Medicine > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From abtdhu at gmail.com Mon Aug 28 13:53:54 2017 From: abtdhu at gmail.com (Dorothy Hu) Date: Mon, 28 Aug 2017 14:53:54 -0400 Subject: [Histonet] Cut FFPE Slides for ISH Message-ID: Hi Jennifer, My ISH slides (FFPE) were stored in -20oC for several months or more than one year and the targets were less abundant. I didn't use RNAscope though, which is more sensitive. I don't know what kind of RNA your researcher try to locate and need to do ISH within 24 hours after cutting? Fresh cut section will yield more signal for sure. I think as long as you have a few copies of genes in your tissue, RNAscope should be able to detect. Dorothy Hu HSDM > Message: 2 > Date: Mon, 28 Aug 2017 15:30:08 +0000 > From: "Jennifer Phinney" > To: "Histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Cut FFPE Slides for ISH > Message-ID: > namprd05.prod.outlook.com> > > Content-Type: text/plain; charset="us-ascii" > > Hello Histonet, > I am hoping someone can point me in the right direction to find some > references for how long FFPE slides intended for ISH can be kept before > staining. I have a researcher who swears their slides have to be stained > within 24 hours of being cut, which does not sound correct to me. I've > used ACD's RNAscope before and their protocol lists the timeframe as 3 > months (so this is a bit of a difference). > > There's a lot of information about fixation times, but I've come up empty > handed for cut slides. > > Thanks everyone! > > Jennifer Phinney QIHCCM > Project Coordinator > Kansas State University > Veterinary Diagnostic Lab > > > From Ana.Maluenda at baker.edu.au Mon Aug 28 19:06:08 2017 From: Ana.Maluenda at baker.edu.au (Ana Maluenda) Date: Tue, 29 Aug 2017 00:06:08 +0000 Subject: [Histonet] Paraformaldehyde Fixed Tissue In-Reply-To: References: Message-ID: Hi Sandra, I haven't myself particularly worked with brain and spinal cord, but majority of my protocols in my old job used fixation in 4% PFA (24 hours at 4-8oC) and routinely process (or transfer to graded EtOH, if not processing immediately). However, our routine process didn't include a first step in 10% NBF, since PFA plays the role of fixation. Therefore, after PFA, we would have 70% EtOH, 80% EtOH, 95% ETOH, 2x EtOH the Xylenes and wax [assuming you are referring to FFPE?]. Also mouse tissue can be small and delicate, so I remember running liver, spleen, kidney and thymus (soft tissues I worked with) in a short cycle (similar to what I would do for biopsies). Hope this helps! Kind regards, Ana Ana Maluenda Research Assistant Atherothrombosis and Vascular Biology Laboratory Baker Heart and Diabetes Institute 75 Commercial Road, Melbourne VIC 3004 P (03) 8532 1359 E Ana.Maluenda at baker.edu.au W www.baker.edu.au -----Original Message----- From: Sandra Cheasty [mailto:sandra.cheasty at wisc.edu] Sent: Tuesday, 29 August 2017 1:30 AM To: Histonet (histonet at lists.utsouthwestern.edu) Subject: [Histonet] Paraformaldehyde Fixed Tissue Hi all, We are having difficulty sectioning mouse tissue, (brain, spinal cord, liver, and spleen), on paraformaldehyde fixed tissue. Has anyone had issues with paraformaldehyde fixed tissue? They were processed routinely, starting in 10% NBF, with other tissues, and we are cutting them at 3u. Thank you! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting privacy at baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg From vanessa.keeton03 at gmail.com Tue Aug 29 09:37:02 2017 From: vanessa.keeton03 at gmail.com (Vanessa Keeton) Date: Tue, 29 Aug 2017 10:37:02 -0400 Subject: [Histonet] CAP checklist question In-Reply-To: References: Message-ID: Good morning fellow histonetters! I was wondering if anyone in VA would know the laws in reference to checklist question ANP. 10016 in regards to the exclusion of submission of tissue and removal of objects for Pathology examination? CAP gave an example where California Department of Health Care Services requires all tissues and objects removed during surgery to be submitted for Pathology examination, unless a specific request is submitted to the state requestinga variance. Any insight would be greatly appreciated. Vanessa Keeton From gu.lang at gmx.at Tue Aug 29 09:51:19 2017 From: gu.lang at gmx.at (Gudrun Lang) Date: Tue, 29 Aug 2017 16:51:19 +0200 Subject: [Histonet] decal protocol with EDTA for bonemarrow biopsies Message-ID: <000c01d320d6$4696e260$d3c4a720$@gmx.at> Dear histonetters! I would be happy about some input about decalcification protocols with EDTA of trephine bonemarrow biopsies. recommended duration of fixation? recommended duration of decalcification? strategies for speed-up of decal? recommended EDTA-solution formula? Hopefully some experienced histotechs can share their knowledge with me. thanks in advance Gudrun From mgudo at me.com Tue Aug 29 09:55:39 2017 From: mgudo at me.com (Michael Gudo) Date: Tue, 29 Aug 2017 16:55:39 +0200 Subject: [Histonet] Paraformaldehyde Fixed Tissue In-Reply-To: References: Message-ID: <09FB1498-5992-43EB-B17D-13889281E1FE@me.com> Dear Sandra, Dear Ana, in our lab for tissues for which we have enough time for infiltration we fix for at least 48 hours or more in NBF or PFA (both with 4 % reactive formaldehyde, which is equivalent to 10% ?neutral buffered formaline?) and then first wash in tap water for a minimum of 1-2 hours, then to 30%, 50%, 60%, 70%, 80%, 90% 96% (2 times), 2-Propanol (2-times) and then 2-3 time xylene, before embedding in paraffine. The times are just a few hours per step or shorter, and we have quite good results especially for brain and spinal chord. Best regards Michael > Am 29.08.2017 um 02:06 schrieb Ana Maluenda via Histonet : > > Hi Sandra, > > I haven't myself particularly worked with brain and spinal cord, but majority of my protocols in my old job used fixation in 4% PFA (24 hours at 4-8oC) and routinely process (or transfer to graded EtOH, if not processing immediately). > However, our routine process didn't include a first step in 10% NBF, since PFA plays the role of fixation. Therefore, after PFA, we would have 70% EtOH, 80% EtOH, 95% ETOH, 2x EtOH the Xylenes and wax [assuming you are referring to FFPE?]. > Also mouse tissue can be small and delicate, so I remember running liver, spleen, kidney and thymus (soft tissues I worked with) in a short cycle (similar to what I would do for biopsies). > > Hope this helps! > > Kind regards, > > Ana > > > Ana Maluenda > Research Assistant > Atherothrombosis and Vascular Biology Laboratory > > Baker Heart and Diabetes Institute > 75 Commercial Road, Melbourne VIC 3004 > P (03) 8532 1359 E Ana.Maluenda at baker.edu.au W www.baker.edu.au > > -----Original Message----- > From: Sandra Cheasty [mailto:sandra.cheasty at wisc.edu] > Sent: Tuesday, 29 August 2017 1:30 AM > To: Histonet (histonet at lists.utsouthwestern.edu) > Subject: [Histonet] Paraformaldehyde Fixed Tissue > > Hi all, > We are having difficulty sectioning mouse tissue, (brain, spinal cord, liver, and spleen), on paraformaldehyde fixed tissue. Has anyone had issues with paraformaldehyde fixed tissue? They were processed routinely, starting in 10% NBF, with other tissues, and we are cutting them at 3u. > Thank you! > Sandy > > Sandra J. Cheasty, HT (ASCP) > Histology & Necropsy Supervisor > UW-Madison, School of Veterinary Medicine > > > Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting privacy at baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a > -- > Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mgudo at me.com Tue Aug 29 09:55:44 2017 From: mgudo at me.com (Michael Gudo) Date: Tue, 29 Aug 2017 16:55:44 +0200 Subject: [Histonet] Cut FFPE Slides for ISH In-Reply-To: References: Message-ID: <668E8BEB-F8F5-4B40-A64D-77714C85035B@me.com> Hello Jenniffer, we store the slides with section at 4-8 ?C for many month or even longer and we do not have any problems with ISH. Best regards Michael > Am 28.08.2017 um 17:30 schrieb Jennifer Phinney via Histonet : > > Hello Histonet, > I am hoping someone can point me in the right direction to find some references for how long FFPE slides intended for ISH can be kept before staining. I have a researcher who swears their slides have to be stained within 24 hours of being cut, which does not sound correct to me. I've used ACD's RNAscope before and their protocol lists the timeframe as 3 months (so this is a bit of a difference). > > There's a lot of information about fixation times, but I've come up empty handed for cut slides. > > Thanks everyone! > > Jennifer Phinney QIHCCM > Project Coordinator > Kansas State University > Veterinary Diagnostic Lab > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Dachel at va.gov Tue Aug 29 10:32:31 2017 From: Susan.Dachel at va.gov (Dachel, Susan K.) Date: Tue, 29 Aug 2017 15:32:31 +0000 Subject: [Histonet] Quality Measures and Productivity Trackers for Anatomic Pathology Program Support Assistants Message-ID: <0FFA32161E88AA42B1B7B0B19B43A54233A34D27@VAPNSMSGD53S01.vha.med.va.gov> Does anyone have any quality measures or productivity trackers that they have used for Anatomic Pathology office staff? I am particularity interested in error rate acceptability and productivity standards that include typing gross and microscopic dictation, entering SNOMED and CPT coding, and entering supplemental or modified reports. Thanks in advance for your help! Sue From Linda.Margraf at cookchildrens.org Tue Aug 29 10:33:14 2017 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Tue, 29 Aug 2017 15:33:14 +0000 Subject: [Histonet] FW: Histonet post In-Reply-To: References: Message-ID: <046f4d3664ea4eeea7e9ccc46427d415@MBXDR10.CCHCS.LDAP> Here is a message I am posting for Samantha. Please reply to her not me, thanks! From: "Green, Samantha" > Date: August 28, 2017 at 3:18:32 PM CDT To: 'Linda Margraf' > Subject: Histonet post Hello Histoneters, I am reaching out to the pathology world in the hopes that someone can help us out. Our lab is currently validating two new staining protocols: 1. HER2 FISH for gastric tissue (stomach & distal esophagus) 2. ALK FISH for lung tissue We have hit a road block without validation because we have run out of patient tissue. We are desperately searching for: ? 10 distal esophagus / gastric tissue POSITIVE for HER2 (preferably stained by FISH previously) ? 5 distal esophagus / gastric tissue NEGATIVE for HER2 (preferably stained by FISH previously) ? 20 specimens (surgical or cytology cell block) POSITIVE for ALK (previously stained by FISH only) We would need two charged slides with 5 micron sections and proof of stain results (example: de-identified report). We understand this is a lot of work but anything thing would help, even if it is just one or two cases. We are not asking anyone to ?work for free? so we are 100% willing to trade help for tissue blocks or purchase the slides if necessary. We do have TONS of FFPE tissue blocks that are HSV I & II positive. I know that HSV can be hard to come by and we would be willing to send some tissue blocks in exchange for the slides listed above. Please either post on this blog or contact me directly at samantha.green at cmc-nh.org . Thanks for reading! Best regards - Samantha Green, B.S. HTL (ASCP) From maxim_71 at mail.ru Tue Aug 29 13:47:45 2017 From: maxim_71 at mail.ru (=?UTF-8?B?0J/QtdGI0LrQvtCyINCc0LDQutGB0LjQvA==?=) Date: Tue, 29 Aug 2017 21:47:45 +0300 Subject: [Histonet] =?utf-8?q?decal_protocol_with_EDTA_for_bone_marrow_bio?= =?utf-8?q?psies?= Message-ID: <1504032465.240798788@f350.i.mail.ru> We fixed our BM biopsies at least 24 hours, but no more than 72 h in 10% NBF. Wash with tap water 30 mins. Decal into 14% EDTA (2Na salt) buffered with NaOH up to 7.0 overnight or weekend and test endpoint by weight loss weight gain method (special thanks to Gayle Callis!). Wash in tap water 1 hour. Process with isopropanols (IPA), mixtures IPA and mineral oil), paraffin wax. Microtomy at 3 microns. Stain HE, Giemsa, HC, IHC. -- Maxim Peshkov, Russia, Taganrog. From schluetertina at googlemail.com Wed Aug 30 05:20:20 2017 From: schluetertina at googlemail.com (=?UTF-8?Q?Tina_Schl=C3=BCter?=) Date: Wed, 30 Aug 2017 12:20:20 +0200 Subject: [Histonet] Help for building up Technovit 7200 and 9100 techniques Message-ID: Hello Histonet-Members I am recently asked from my lab-coordinator to establish cutting-grinding techniques with the technovit-resins for sectioning bones and other hard-tissue materials. I do not have many experiences in these techniques and I am looking for some help. And also, where can I buy buy these resins and consumables. As far as I know, Heraeus Kulzer has a new global distributor for this resins? Could anybody help? Does anybody know about trainings and courses in using these resins and establishing the methods? Thanks in advance for any help Tina From Erin.Martin at ucsf.edu Wed Aug 30 18:44:22 2017 From: Erin.Martin at ucsf.edu (Martin, Erin) Date: Wed, 30 Aug 2017 23:44:22 +0000 Subject: [Histonet] Workload tracking Message-ID: Hello all, Would anyone be willing to share how they track workload? Spreadsheet? Database? Our volume has recently increased and I am looking for a way to track individual output so that I can distribute work and rotate tasks fairly. I don't want to have my "fast" people going out with repetitive motion injuries because they ended up with an unfair portion of the workload. Thank you in advance for any suggestions! Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. From gayle.callis at bresnan.net Thu Aug 31 11:01:43 2017 From: gayle.callis at bresnan.net (Gayle Callis) Date: Thu, 31 Aug 2017 10:01:43 -0600 Subject: [Histonet] Attention Gudrun Lang Re EDTA method for bone marrow biopsies Message-ID: <001001d32272$71293b20$537bb160$@bresnan.net> Dear Gudrun, EDTA will always be slower than a buffered formic acid and there are caveats about using it for some enzyme methods for bone. This EDTA method which is better than most but is avoid pH that is too low. This EDTA method is made with tetra-sodium EDTA (Fisher BP 121-500 which has a very high molecular weight). The pH is adjusted DOWN to pH of your IHC buffers. I have a review of decalcification which explains how EDTA decalcifies bone as a function of pH. The correct EDTA with a high molecular weight information is also attached. The method was developed by a bone guru. Webb Gee, many years ago, and has remained a favorite of mine and others for many years. You might get by decalcifying in 24 hours with the higher concentration of EDTA but there is no true way to speed it up. We used acetic acid to adjust the pH down since this EDTA is very alkaline but is highly soluble. HCL can be used to adjust the pH. I liked pH 7.6 which alone, speed up the rate a bit, and this is the same pH as TRIS buffered saline. I made up my EDTA in Dulbecco's PBS, or any PBS will work. You decalcify at RT. EDTA is not affected by temperature since it is a chelator. A trick to help you is cut off the cortical bone plug at some point during decalcification since pathologists should be interested in only the trabecular bone with marrow components. This will speed up decal too. METHOD: 14% EDTA Tetrasodium DECALCIFICATION (Webb Jee, Stain Technology) 140 g EDTA, tetrasodium salt in 800 ml distilled water. Dulbeccos PBS (DPBS) is preferred over distilled water. Starting pH is approx. 11, very alkaline and could damage alkaline sensitive protein linkages. Hence using a pH close to your IHC buffers is desirable. Adjust with glacial acetic acid (or HCL) using pH meter, mechanical stirrer and continuous readout on pH meter. It takes approximately 18 ml glacial acetic acid for every liter of 14% EDTA (tetrasodium salt). Since one adds a large volume of glacial acetic acid at beginning, start out with suggested gm of EDTA in less buffer,i.e. 800 ml, adjust the pH down. When pH 7.4- 7.6 reached, bring final volume to 1 liter using the solvent i.e. water, DPBS or PBS. This is basically a titration technique with continuous readout on a pH meter. Suspend bone in decalcifying solution and stir or rock gently. This decalcifying method is NOT used for articular or other cartilage work since EDTA extracts proteoglycans and will change the tinctorial quality of cartilage stains i.e. Toluidine Blue, and Safranin O/Fast green so these appear weaker. It can extract proteoglycans needed for IHC. Samples can be left in EDTA solution over a weekend, but change frequently to replenish active decalcifying agent EDTA. Use a sufficient volume, 20:1 for adequate decalcification. I have a colleague using this EDTA method for murine bones, and she loves it even for doing decalcified bone frozen sections. Contact me privately and I will send a publication explaining how EDTA works chemically as a function of pH, Webb Jee publication and the weight loss/weight gain decalcification endpoint method. Take care, and stay in touch. Gayle You wrote: I would be happy about some input about decalcification protocols with EDTA of trephine bone marrow biopsies. recommended duration of fixation? Complete fixation. NBF totally fixes in 24 h but you may not be able to wait that long. recommended duration of decalcification? Maybe 24 h, less is debatable. EDTA will always be slow since it is a chelation process. There is a weight loss/weight gain decalcification end point test, highly advisable, to know when sample is decalcified. This requires a balance which weighs in mg, or you can use xray method. A chemical method for EDTA is painfully tedious. strategies for speed-up of decal? Use higher concentration 14% EDTA, tetrasodium at pH 7.6 with some gentle agitation. recommended EDTA-solution formula? Provided above it 14% Tetrasodium EDTA, pH 7.6. This is highly alkaline EDTA and the pH is adjusted down with acetic or HCl. Hopefully some experienced histotechs can share their knowledge with me. thanks in advance Gudrun From patpxs at gmail.com Thu Aug 31 13:07:43 2017 From: patpxs at gmail.com (P Sicurello) Date: Thu, 31 Aug 2017 11:07:43 -0700 Subject: [Histonet] Recut rates Message-ID: Good Morning Listers, What is the recut rate you all are experiencing? You can include deepers in that count, though I'm more interested in recuts due to the first H&E not being good enough. Not faced into well enough to expose all the tissue in the block, that type of thing. I'm trying to figure out what's the average recut rate for a lab that processes 400-700 blocks a day. Thanks in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer.