[Histonet] hirsch-peiffer cresyl violet method?

[Jennifer MacDonald]

Tim,
I have this method in one of medical technology books from many years ago. 
 Lynch's Medical Laboratory Technology II.  The author of the Histology 
section was Charles F. A. Culling.  There are two procedures, one for 
urine and one for nervous system tissue.  I assume you want the nervous 
system?

Procedure:
1.  Cut 8 to 12 um frozen sections of formalin-fixed tissue into distilled 
water.
2. Stain free-floating sections in 1% cresyl echt violet in 1% acetic acid 
(pH about 2.7) for 10 minutes (pH 3.5 to 3.6 is preferable) as with urine.
3. Rinse in distilled water and mount in glycerin.

Results
Granules of metachromatic leukodystrophy - golden brown that remains 
uncvhanged after treatment with 1% ammonium water.
The granules stain positively with PAS in paraffin sections, but the 
present author has fuond that the PAS-positive reaction faded in routinely 
mounted sections after 6 to 12 months.

I can scan the page for you if you'd like.
Jennifer



From:   "Morken, Timothy via Histonet" <histonet at lists.utsouthwestern.edu>
To:     Histonet <histonet at lists.utsouthwestern.edu>
Date:   04/14/2017 11:45 AM
Subject:        [Histonet] hirsch-peiffer cresyl violet  method?



Hi all,

Does anyone have the method for Hirsch-peiffer cresyl violet used  for 
metachromatic leukodystrophy? On frozen sections.

We are trying to find out if it is different than the usual cresyl violet 
with acetic acid method for Nissl bodies.

I've found many references to it, but none that give the procedure. And 
the original paper is from 1955 not easily available. And is in German....



Thanks for any help.

Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

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