From ajayiifeoluwa at gmail.com Sat Apr 1 07:11:44 2017 From: ajayiifeoluwa at gmail.com (Ifeoluwa Ajayi) Date: Sat, 1 Apr 2017 13:11:44 +0100 Subject: [Histonet] Survey!!!!!! In-Reply-To: <15b25bbc879-4891-5749@webprd-a21.mail.aol.com> References: <15b25bbc879-4891-5749@webprd-a21.mail.aol.com> Message-ID: Personally I will prefer auto coverslipper, because cover slipping is cumbersome and takes time. Ajayi Ifeoluwa, BMLS, AMLSCN, MSc, UCH Ibadan, Nigeria. On Mar 31, 2017 8:00 PM, "Patti Nelson - PNP Lab Consultant via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > > Hi Everyone, > > I just wanted to get everyone's opinion. If you had to chose between > buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you > chose? Lets say your volume was around 60 to 80 blocks a day and you worked > for a GI Lab. Everyone's input would be greatly appreciated. > > > > Sincerely, > > PATTI NELSON H.T.(ASCP) > PNP LABORATORY CONSULTANTS > SUPERVISOR DGC/ZADEH LABS > PO BOX 412 > CABAZON, CA. 92230 > 909-841-9761 > nelsonrnch at verizon.net > CONFIDENTIALITY NOTICE:This message and any included attachments are from > Patti Nelson, PNP Laboratory Consultants and are intended only for the > addressee. The information contained in this message is confidential and > may contain privileged, confidential, proprietary and/or exemption from > disclosure under applicable law. Unauthorized forwarding, printing, > copying, distribution, or use of such information is strictly prohibited > and may be unlawful. If you are not the addressee, please promptly delete > this message and notify the sender ofthe delivery error by e-mail or you > may call 909-841-9761. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pruegghm at hotmail.com Sat Apr 1 18:18:49 2017 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Sat, 1 Apr 2017 23:18:49 +0000 Subject: [Histonet] Survey!!!!!! In-Reply-To: References: <15b25bbc879-4891-5749@webprd-a21.mail.aol.com>, Message-ID: I have seen so many more problems with auto coverslippers, they break down, the cover does not hold up over time for archiving, etc., that I would would probably chose an autostainer. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Ifeoluwa Ajayi Sent: Saturday, April 1, 2017 6:11 AM To: Patti Nelson - PNP Lab Consultant Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Survey!!!!!! Personally I will prefer auto coverslipper, because cover slipping is cumbersome and takes time. Ajayi Ifeoluwa, BMLS, AMLSCN, MSc, UCH Ibadan, Nigeria. On Mar 31, 2017 8:00 PM, "Patti Nelson - PNP Lab Consultant via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > > Hi Everyone, > > I just wanted to get everyone's opinion. If you had to chose between > buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you > chose? Lets say your volume was around 60 to 80 blocks a day and you worked > for a GI Lab. Everyone's input would be greatly appreciated. > > > > Sincerely, > > PATTI NELSON H.T.(ASCP) > PNP LABORATORY CONSULTANTS > SUPERVISOR DGC/ZADEH LABS > PO BOX 412 > CABAZON, CA. 92230 > 909-841-9761 > nelsonrnch at verizon.net > CONFIDENTIALITY NOTICE:This message and any included attachments are from > Patti Nelson, PNP Laboratory Consultants and are intended only for the > addressee. The information contained in this message is confidential and > may contain privileged, confidential, proprietary and/or exemption from > disclosure under applicable law. Unauthorized forwarding, printing, > copying, distribution, or use of such information is strictly prohibited > and may be unlawful. If you are not the addressee, please promptly delete > this message and notify the sender ofthe delivery error by e-mail or you > may call 909-841-9761. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet > From rjbuesa at yahoo.com Sun Apr 2 09:03:02 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Sun, 2 Apr 2017 14:03:02 +0000 (UTC) Subject: [Histonet] Survey!!!!!! In-Reply-To: References: <15b25bbc879-4891-5749@webprd-a21.mail.aol.com> Message-ID: <1790187348.9333912.1491141782554@mail.yahoo.com> Staining is a more complex step than coverslipping. Also a coverslip on a section has more "latitude" and can, if necessary, be removed and coverslip in a different way. A stained section, on the other hand, is almost impossible to "correct". Between an autostainer and a coverslipper, I would choose an autostainer.Ren? On Saturday, April 1, 2017 7:29 PM, Patsy Ruegg via Histonet wrote: I have seen so many more problems with auto coverslippers, they break down, the cover does not hold up over time for archiving, etc., that I would would probably chose an autostainer. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Ifeoluwa Ajayi Sent: Saturday, April 1, 2017 6:11 AM To: Patti Nelson - PNP Lab Consultant Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Survey!!!!!! Personally I will prefer auto coverslipper, because cover slipping is cumbersome and takes time. Ajayi Ifeoluwa, BMLS, AMLSCN, MSc, UCH Ibadan, Nigeria. On Mar 31, 2017 8:00 PM, "Patti Nelson - PNP Lab Consultant via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > > Hi Everyone, > > I just wanted to get everyone's opinion. If you had to chose between > buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you > chose? Lets say your volume was around 60 to 80 blocks a day and you worked > for a GI Lab. Everyone's input would be greatly appreciated. > > > > Sincerely, > > PATTI NELSON? H.T.(ASCP) > PNP LABORATORY CONSULTANTS > SUPERVISOR DGC/ZADEH LABS > PO BOX 412 > CABAZON, CA. 92230 > 909-841-9761 > nelsonrnch at verizon.net > CONFIDENTIALITY NOTICE:This message and any included attachments are from > Patti Nelson, PNP Laboratory Consultants and are intended only for the > addressee. The information contained in this message is confidential and > may contain privileged, confidential, proprietary and/or exemption from > disclosure under applicable law.? Unauthorized forwarding, printing, > copying, distribution, or use of such information is strictly prohibited > and may be unlawful.? If you are not the addressee, please promptly delete > this message and notify the sender ofthe delivery error by e-mail or you > may call? 909-841-9761. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From john.frazier at roche.com Sun Apr 2 12:32:09 2017 From: john.frazier at roche.com (Frazier, John) Date: Sun, 2 Apr 2017 13:32:09 -0400 Subject: [Histonet] Survey!!!!!! Message-ID: <2054628825587975988@unknownmsgid> The one thing I would do is to make sure you choose a glass coverslip. Plastic is not good over time and varying temperatures. Sent from my iPad > On Apr 1, 2017, at 7:18 PM, Patsy Ruegg wrote: > > I have seen so many more problems with auto coverslippers, they break down, the cover does not hold up over time for archiving, etc., that I would would probably chose an autostainer. > > > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > pruegghm at hotmail.com > > > > ________________________________ > From: Ifeoluwa Ajayi > Sent: Saturday, April 1, 2017 6:11 AM > To: Patti Nelson - PNP Lab Consultant > Cc: Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Survey!!!!!! > > Personally I will prefer auto coverslipper, because cover slipping is > cumbersome and takes time. > > Ajayi Ifeoluwa, BMLS, AMLSCN, MSc, > UCH Ibadan, Nigeria. > > On Mar 31, 2017 8:00 PM, "Patti Nelson - PNP Lab Consultant via Histonet" < > histonet at lists.utsouthwestern.edu> wrote: > >> >> Hi Everyone, >> >> I just wanted to get everyone's opinion. If you had to chose between >> buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you >> chose? Lets say your volume was around 60 to 80 blocks a day and you worked >> for a GI Lab. Everyone's input would be greatly appreciated. >> >> >> >> Sincerely, >> >> PATTI NELSON H.T.(ASCP) >> PNP LABORATORY CONSULTANTS >> SUPERVISOR DGC/ZADEH LABS >> PO BOX 412 >> CABAZON, CA. 92230 >> 909-841-9761 >> nelsonrnch at verizon.net >> CONFIDENTIALITY NOTICE:This message and any included attachments are from >> Patti Nelson, PNP Laboratory Consultants and are intended only for the >> addressee. The information contained in this message is confidential and >> may contain privileged, confidential, proprietary and/or exemption from >> disclosure under applicable law. Unauthorized forwarding, printing, >> copying, distribution, or use of such information is strictly prohibited >> and may be unlawful. If you are not the addressee, please promptly delete >> this message and notify the sender ofthe delivery error by e-mail or you >> may call 909-841-9761. >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Histonet Info Page - lists.utsouthwestern.edu Mailing Lists > lists.utsouthwestern.edu > Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet > > >> > > From badams at acadianagastro.com Sun Apr 2 13:42:30 2017 From: badams at acadianagastro.com (Brent Adams) Date: Sun, 2 Apr 2017 18:42:30 +0000 Subject: [Histonet] Histonet Digest, Vol 161, Issue 2, "SURVEY" In-Reply-To: References: Message-ID: In our GI Laboratory we have both and we do between 35 and 55 blocks per day. I would not want to have to choose between the both. I would get a "new" Auto stainer because the quality of your stained slides would be more consistent and be easier to assess for QC purposes. I would get a "used" Tissue-Tek coverslip machine because it is a work horse tank and rarely has problems and would increase productivity by 5x over doing hand coverslips as well as reduce the chemical hazards exposure to the Tech. Brent Adams ? BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Sunday, April 2, 2017 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 161, Issue 2 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Survey!!!!!! (Patsy Ruegg) 2. Re: Survey!!!!!! (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Sat, 1 Apr 2017 23:18:49 +0000 From: Patsy Ruegg To: Ifeoluwa Ajayi , "Patti Nelson - PNP Lab Consultant" Cc: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Survey!!!!!! Message-ID: Content-Type: text/plain; charset="iso-8859-1" I have seen so many more problems with auto coverslippers, they break down, the cover does not hold up over time for archiving, etc., that I would would probably chose an autostainer. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Ifeoluwa Ajayi Sent: Saturday, April 1, 2017 6:11 AM To: Patti Nelson - PNP Lab Consultant Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Survey!!!!!! Personally I will prefer auto coverslipper, because cover slipping is cumbersome and takes time. Ajayi Ifeoluwa, BMLS, AMLSCN, MSc, UCH Ibadan, Nigeria. On Mar 31, 2017 8:00 PM, "Patti Nelson - PNP Lab Consultant via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > > Hi Everyone, > > I just wanted to get everyone's opinion. If you had to chose between > buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you > chose? Lets say your volume was around 60 to 80 blocks a day and you worked > for a GI Lab. Everyone's input would be greatly appreciated. > > > > Sincerely, > > PATTI NELSON H.T.(ASCP) > PNP LABORATORY CONSULTANTS > SUPERVISOR DGC/ZADEH LABS > PO BOX 412 > CABAZON, CA. 92230 > 909-841-9761 > nelsonrnch at verizon.net > CONFIDENTIALITY NOTICE:This message and any included attachments are from > Patti Nelson, PNP Laboratory Consultants and are intended only for the > addressee. The information contained in this message is confidential and > may contain privileged, confidential, proprietary and/or exemption from > disclosure under applicable law. Unauthorized forwarding, printing, > copying, distribution, or use of such information is strictly prohibited > and may be unlawful. If you are not the addressee, please promptly delete > this message and notify the sender ofthe delivery error by e-mail or you > may call 909-841-9761. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet > ------------------------------ Message: 2 Date: Sun, 2 Apr 2017 14:03:02 +0000 (UTC) From: Rene J Buesa To: Patsy Ruegg , Ifeoluwa Ajayi , Patti Nelson - PNP Lab Consultant Cc: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Survey!!!!!! Message-ID: <1790187348.9333912.1491141782554 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Staining is a more complex step than coverslipping. Also a coverslip on a section has more "latitude" and can, if necessary, be removed and coverslip in a different way. A stained section, on the other hand, is almost impossible to "correct". Between an autostainer and a coverslipper, I would choose an autostainer.Ren? On Saturday, April 1, 2017 7:29 PM, Patsy Ruegg via Histonet wrote: I have seen so many more problems with auto coverslippers, they break down, the cover does not hold up over time for archiving, etc., that I would would probably chose an autostainer. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Ifeoluwa Ajayi Sent: Saturday, April 1, 2017 6:11 AM To: Patti Nelson - PNP Lab Consultant Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Survey!!!!!! Personally I will prefer auto coverslipper, because cover slipping is cumbersome and takes time. Ajayi Ifeoluwa, BMLS, AMLSCN, MSc, UCH Ibadan, Nigeria. On Mar 31, 2017 8:00 PM, "Patti Nelson - PNP Lab Consultant via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > > Hi Everyone, > > I just wanted to get everyone's opinion. If you had to chose between > buying a Auto Side Stainer or Auto Slide Cover Slipper, which one would you > chose? Lets say your volume was around 60 to 80 blocks a day and you worked > for a GI Lab. Everyone's input would be greatly appreciated. > > > > Sincerely, > > PATTI NELSON? H.T.(ASCP) > PNP LABORATORY CONSULTANTS > SUPERVISOR DGC/ZADEH LABS > PO BOX 412 > CABAZON, CA. 92230 > 909-841-9761 > nelsonrnch at verizon.net > CONFIDENTIALITY NOTICE:This message and any included attachments are from > Patti Nelson, PNP Laboratory Consultants and are intended only for the > addressee. The information contained in this message is confidential and > may contain privileged, confidential, proprietary and/or exemption from > disclosure under applicable law.? Unauthorized forwarding, printing, > copying, distribution, or use of such information is strictly prohibited > and may be unlawful.? If you are not the addressee, please promptly delete > this message and notify the sender ofthe delivery error by e-mail or you > may call? 909-841-9761. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists Histonet Info Page - lists.utsouthwestern.edu Mailing Lists lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet ------------------------------ End of Histonet Digest, Vol 161, Issue 2 **************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. From tony.henwood at health.nsw.gov.au Sun Apr 2 19:57:53 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon, 3 Apr 2017 00:57:53 +0000 Subject: [Histonet] Tissue Fixation In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF95539750@SVDCMBX-MEX008.nswhealth.net> If the specimen is placed on absorbent paper prior to fixation, the paper will gradually absorb the water from the specimen causing it to dry out. Microscopically the tissue will look a little like what you see when a laser scalpel is used to excise a skin specimen. The heat affected eosin stained collagen will look quite brilliant (even fluorescent like) and if you do a Masson stain for connective tissue will give a red colouration, rather than a green or blue you would normally see with well-fixed collagen. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: T H via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 31 March 2017 11:51 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Tissue Fixation Good Morning, I have a pathologist that is not happy with the fixation on some of our LEEP specimens. She swears its histology doing something to the specimen to cause the tissue to look unfixed on only "part" of the LEEP specimens (all the same client specimens). She claims we must be diluting our formalin to cause this issue or "something". We mentioned maybe it was on the clients end not placing them in 10% formalin right away, she wouldn't hear of it. Let me give you some back ground on how our process works. Our clients send us all our specimens to us via Overnight FedEx or UPS in 10% formalin they will then they sit in 10% formalin in-house until the processors are started around 3pm and sits an additional 5 hours in 10% formalin on the processor before the processor actually starts. That being said the fixation process has had a pretty good start before we ever even touch it. My question is, and I thought I had read this in the past is, when a specimen is left out prior to fixation and lying on a absorbent surface such as a paper towel, won't the area of the tissue touching the absorbent surface begin the disintegration processes faster in that exact area then the rest of the specimen? Or if you have any other suggestion on what might be happening to only "certain" specimens would be great as well. Thanks for your help! Tim _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From tbraud at holyredeemer.com Mon Apr 3 09:35:27 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 3 Apr 2017 14:35:27 +0000 Subject: [Histonet] Stainer vs coverslipper Message-ID: <48E053DDF6CE074DB6A7414BA05403F812E600@HRHEX03-HOS.holyredeemer.local> Such a shame that you have to choose. But, that said, a coverslipper. Hands down. If nothing else, due to safety and exposure reasons alone. H and E stains can be unstained and restained, easily corrected, and easily accomplished in batches and the racks have HANDLES. But with coverslipping by hand, there is no way to avoid contact with solvents, even if using Nitrile gloves, which eventually break down in any solvent. I've used good auto coverslippers and bad ones, but ask any of my techs (and I did) and they would rather give up the stainer than the coverslipper. We've had our Sakura Glas now for almost 10 years with virtually no problems. The ability to load 60 slides and walk away to come back to perfectly coverslipped slides is a blessing. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From TNMayer at mdanderson.org Mon Apr 3 14:52:34 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Mon, 3 Apr 2017 19:52:34 +0000 Subject: [Histonet] Survey Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8839EA3A30@D1PWPEXMBX08.mdanderson.edu> In my opinion a stainer would come first. As it has already been stated, stainers provide consistency and reliability in the lab. You can always find someone to assist with coverslipping, but not everyone can perform the stain just right. Newer coverslippers are wonderful, they last and work great. Just remember to open the dispensing valve for adequate flow of the reagent onto the slide. That has always been the biggest problem with film units. Don't get chintzsy with the reagent, but don't let them be too juicy. > > On Mar 31, 2017 8:00 PM, "Patti Nelson - PNP Lab Consultant via > Histonet" < histonet at lists.utsouthwestern.edu> wrote: > >> >> Hi Everyone, >> >> I just wanted to get everyone's opinion. If you had to chose between >> buying a Auto Side Stainer or Auto Slide Cover Slipper, which one >> would you chose? Lets say your volume was around 60 to 80 blocks a >> day and you worked for a GI Lab. Everyone's input would be greatly appreciated. >> >> >> >> Sincerely, >> >> PATTI NELSON H.T.(ASCP) >> PNP LABORATORY CONSULTANTS >> SUPERVISOR DGC/ZADEH LABS >> PO BOX 412 >> CABAZON, CA. 92230 >> 909-841-9761 >> nelsonrnch at verizon.net >> CONFIDENTIALITY NOTICE:This message and any included attachments are >> from Patti Nelson, PNP Laboratory Consultants and are intended only >> for the addressee. The information contained in this message is >> confidential and may contain privileged, confidential, proprietary >> and/or exemption from disclosure under applicable law. Unauthorized >> forwarding, printing, copying, distribution, or use of such >> information is strictly prohibited and may be unlawful. If you are >> not the addressee, please promptly delete this message and notify the >> sender ofthe delivery error by e-mail or you may call 909-841-9761. >> The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From criley at dpspa.com Tue Apr 4 09:05:03 2017 From: criley at dpspa.com (Charles Riley) Date: Tue, 4 Apr 2017 10:05:03 -0400 Subject: [Histonet] Processing cystic tissue Message-ID: Can anyone give me an idea how they process cystic tissues. THe normal tissue processes extremely well on my current protocol but the cystic areas just are too soft to cut. Any tricks anyone can provide to process better or cut better after processing would be greatly appreciated -- Charles Riley HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From relia1 at earthlink.net Tue Apr 4 09:53:44 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 4 Apr 2017 10:53:44 -0400 Subject: [Histonet] RELIA Special Job Alert for Managers and Supervisors 4/4/2017 Exciting opportunities Nationwide!!! Message-ID: <0f3c01d2ad53$4275c170$c7614450$@earthlink.net> Hello Histonetters, I have several exciting opportunities for experienced Managers, Supervisors and Lead Techs in hospital and private lab environments in several locations throughout the US. These are some of the premier employers in the United States. The positions are of course full time and permanent. My clients offer excellent compensation, benefits and relocation assistance. Here are my leadership positions: Histology Supervisor - Madison, WI Histology Manager - San Diego, CA Lab Manager - Danville, VA Lead Histotech - Vancouver, WA I anticipate multiple management openings with many exciting opportunities for talented managers and supervisor to be coming available nationwide as many of my clients are experiencing rapid growth again. If you are interested in looking into making a job change please let me know. That way I can keep you apprised of management opportunities that would interest you. Also if you would like more information or know of someone else who might be interested, please contact me at relia1 at earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam There are a lot of recruiters out there right now trying to work with histology professionals and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 15 years I have dedicated my practice solely to placing histology professionals like you. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From CIngles at uwhealth.org Tue Apr 4 17:15:18 2017 From: CIngles at uwhealth.org (Ingles Claire) Date: Tue, 4 Apr 2017 22:15:18 +0000 Subject: [Histonet] Processing cystic tissue In-Reply-To: References: Message-ID: Charles: I usually get out as much of the cystic material as I can without damaging the cyst wall itself. That is all the docs are looking at anyway. I am assuming you are referring to skin cysts, not ovaries, etc. Claire ________________________________________ From: Charles Riley via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, April 04, 2017 9:05 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Processing cystic tissue Can anyone give me an idea how they process cystic tissues. THe normal tissue processes extremely well on my current protocol but the cystic areas just are too soft to cut. Any tricks anyone can provide to process better or cut better after processing would be greatly appreciated -- Charles Riley HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From samantha_claypool at hotmail.com Tue Apr 4 20:54:06 2017 From: samantha_claypool at hotmail.com (Samantha Claypool) Date: Wed, 5 Apr 2017 01:54:06 +0000 Subject: [Histonet] Sigma p16 Message-ID: Hello Histonet! Anyone out there using (or getting ready to use) the p16(ink4a) antibody available from Sigma? We currently use Ventana's but will probably buy from Sigma once the Ventana product is discontinued. Would love to hear from anyone with experience with Sigma. Thanks, Samantha Huyer Laboratory Services From BZIMMERM at augusta.edu Wed Apr 5 10:50:52 2017 From: BZIMMERM at augusta.edu (Zimmerman, Billie) Date: Wed, 5 Apr 2017 15:50:52 +0000 Subject: [Histonet] GSH HISTOPALOOZA APRIL 21 - APRIL 22 LEGACY LODGE @LAKE LANIER Message-ID: Histopalooza is fast approaching. The Roche Navigator will be there. This mobile showcase will be featuring the Ventana portfolio of automated staining platforms, specimen tracking, and digital pathology solutions. Hope all of you can make the trip to Lake Lanier. Contact your Ventana rep to set up your time for touring the Navigator. The Navigator is a big mack daddy bus, no pop-up camper. Haha Hopefully, next week I'll have a good link for you to see this. See you soon, Billie Zimmerman From tejohnson at genoptix.com Wed Apr 5 14:47:39 2017 From: tejohnson at genoptix.com (Teri Johnson) Date: Wed, 5 Apr 2017 19:47:39 +0000 Subject: [Histonet] p16 from Ventana Message-ID: Wait...what? When is Ventana p16 being discontinued? Teri Johnson, HT(ASCP)QIHC Manager, Clinical Trial Testing T +1 760 516 5954 tejohnson at genoptix.com Navigate BioPharma, Inc. A Novartis Company 1890 Rutherford Rd. Carlsbad, CA 92008 USA Date: Wed, 5 Apr 2017 01:54:06 +0000 From: Samantha Claypool To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Sigma p16 Hello Histonet! Anyone out there using (or getting ready to use) the p16(ink4a) antibody available from Sigma? We currently use Ventana's but will probably buy from Sigma once the Ventana product is discontinued. Would love to hear from anyone with experience with Sigma. Thanks, Samantha Huyer Laboratory Services ________________________________ CONFIDENTIALITY NOTICE: The sender of this email is not an employee or agent of, or a contractor or consultant to, Genoptix. This email has been sent through the Genoptix email system as a transitional service provided by Genoptix, Inc. (?Genoptix?) to Navigate BioPharma Services, Inc. The sender of this email is not authorized to represent or to speak on behalf of any member of Genoptix, nor to enter into any undertaking, arrangement or agreement on behalf of any member of Genoptix. Any such undertaking, arrangement or agreement shall not be binding on Genoptix. Genoptix accepts no liability for the content of this email, or for the consequences of any acts or omissions taken or not taken on the basis of the information contained in this email or in any email sent from this email address. All reasonable precautions have been taken to ensure no viruses or other malware are present in this e-mail and Genoptix cannot accept responsibility for any loss or damage arising from the use of this e-mail or attachments and you are responsible for ensuring that your own virus screening procedures are up to date and fit for purpose. From mneglia at trajanscimed.com Wed Apr 5 15:12:29 2017 From: mneglia at trajanscimed.com (Marc Neglia) Date: Thu, 6 Apr 2017 06:12:29 +1000 Subject: [Histonet] Pathology slide survey - AMAZON GIFT CARD OFFER In-Reply-To: <61E7E1B920537D4BABCCDF8D8B6E70D1062B2A2FD9E3@mifune1> References: <61E7E1B920537D4BABCCDF8D8B6E70D1062B2A2FD9E3@mifune1> Message-ID: <61E7E1B920537D4BABCCDF8D8B6E70D1062B2A36E464@mifune1> REMINDER: Survey is still active. Respond now to be entered into the drawing. Hello Histonetters, I am conducting a study to learn about your experiences in the laboratory with microscope slides. The information gathered will help assist with product development decisions that are aimed at improving patient outcomes and optimizing your pathology workflow. To participate in this short, 5 minute survey, please click on the link below: https://www.surveymonkey.com/r/BKNBBWV As a token of appreciation, we will randomly select four (4) respondents to receive a $50 Amazon gift card after the survey has been completed. I would like to thank you in advance for your participation, and I look forward to viewing your responses. Best regards, Marc Marc Neglia Trajan Scientific and Medical mneglia at trajanscimed.com www.trajanscimed.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Wed Apr 5 15:53:21 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Wed, 5 Apr 2017 16:53:21 -0400 Subject: [Histonet] Processing cystic tissue Message-ID: Charles Riley HT, HTL(ASCP)CM, a Mohs histopathology coordinator, asks: >>Can anyone give me an idea how they process cystic tissues? The normal tissue processes extremely well on my current protocol but the cystic areas just are too soft to cut. Any tricks anyone can provide to process better or cut better after processing would be greatly appreciated.<< Whoever's grossing should remove most of the keratinous contents of an epidermal inclusion cyst (or the contents of a pilar cyst), leaving a small amount around the edge so that you don't disrupt the lining much. That's what I do for myself. There's nothing to see in the keratin, and it interferes with getting a good section. The same reasoning applies to other cystic material, such as ovarian cysts. Bob Richmond Samurai Pathologist Maryville TN From kenia.lynch.kl1 at roche.com Wed Apr 5 16:07:46 2017 From: kenia.lynch.kl1 at roche.com (Lynch, Kenia) Date: Wed, 5 Apr 2017 17:07:46 -0400 Subject: [Histonet] p16 from Ventana In-Reply-To: References: Message-ID: Hi all, Roche will still have the p16 antibody, but there are a few changes taking place. Please reach out to your account manager for clarification. Thanks, KCL On Wed, Apr 5, 2017 at 3:47 PM, Teri Johnson via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Wait...what? When is Ventana p16 being discontinued? > > Teri Johnson, HT(ASCP)QIHC > Manager, Clinical Trial Testing > > T +1 760 516 5954 > tejohnson at genoptix.com > > Navigate BioPharma, Inc. > A Novartis Company > 1890 Rutherford Rd. > Carlsbad, CA 92008 > USA > > Date: Wed, 5 Apr 2017 01:54:06 +0000 > From: Samantha Claypool > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Sigma p16 > > Hello Histonet! Anyone out there using (or getting ready to use) the > p16(ink4a) antibody available from Sigma? We currently use Ventana's but > will probably buy from Sigma once the Ventana product is discontinued. > Would love to hear from anyone with experience with Sigma. > > Thanks, > Samantha > Huyer Laboratory Services > > > > > ________________________________ > > CONFIDENTIALITY NOTICE: The sender of this email is not an employee or > agent of, or a contractor or consultant to, Genoptix. This email has been > sent through the Genoptix email system as a transitional service provided > by Genoptix, Inc. (?Genoptix?) to Navigate BioPharma Services, Inc. The > sender of this email is not authorized to represent or to speak on behalf > of any member of Genoptix, nor to enter into any undertaking, arrangement > or agreement on behalf of any member of Genoptix. Any such undertaking, > arrangement or agreement shall not be binding on Genoptix. Genoptix accepts > no liability for the content of this email, or for the consequences of any > acts or omissions taken or not taken on the basis of the information > contained in this email or in any email sent from this email address. All > reasonable precautions have been taken to ensure no viruses or other > malware are present in this e-mail and Genoptix cannot accept > responsibility for any loss or damage arising from the use of this e-mail > or attachments and you are responsible for ensuring that your own virus > screening procedures are up to date and fit for purpose. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *Kenia C. Lynch, MT, HT, QIHC* Account Manager, North Carolina Roche Diagnostics Corporation 9115 Hague Road, PO Box 50457 Indianapolis, IN 46250-0457 Tel. +1-800-227-2155 Cell +1-208-972-4088 kenia.lynch.kl1 at roche.com [image: The Future of H&E] *Confidentiality Note:* This message is intended only for the use of the named recipient(s) and may contain confidential and/or proprietary information. If you are not the intended recipient, please contact the sender and delete this message. Any unauthorized use of the information contained in this message is prohibited. From SteveM at mcclainlab.com Thu Apr 6 05:38:18 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Thu, 6 Apr 2017 10:38:18 +0000 Subject: [Histonet] Histonet Digest, Vol 161, Issue 5Subject: Re: Processing cystic tissue In-Reply-To: References: Message-ID: <8A5F5568-8B3E-438E-8D04-D57D97CE0EF2@mcclainlab.com> Skin cysts are difficult for everyone. Cysts and toenails lead the top of our list of cases with catastrophic tissue loss - which we define as tissue loss w "holes" >1mm2 square. The cyst center generally does not fix readily, composed of unfixed cornified cells and waxy lipid. On the scalp, wens or isthmus-catagen cysts are also calcified. The outer aspect may include dermis and scar and inflammation and also the squamous cyst lining. All of these factors limit diffusion and penetration of fixatives and processing alcohols/clearants/paraffins. We open all cysts by slicing into them and allow them to fix another 6-8 hours in fresh 10%NBF, then process on a long cycle of 12-16 hours, beginning in 50% alcohol in station 1. 12 hours is our standard overnight cycle and 16 hours is used for toenails. Our cyst slides are still not perfect, but are sufficient to make a photomicrograph-our usual method-we photograph every specimen. Steve A. McClain, MD > On Apr 5, 2017, at 13:23, "histonet-request at lists.utsouthwestern.edu" wrote: > > Subject: Re: [Histonet] Processing cystic tissue From relia1 at earthlink.net Thu Apr 6 09:11:43 2017 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 6 Apr 2017 10:11:43 -0400 Subject: [Histonet] Histology Supervisor needed - Pharm/Research Chicagoland area. A RELIA EXCLUSIVE!! Can you help? Message-ID: <019501d2aedf$bab1cc00$30156400$@earthlink.net> Hi Histonetters, How are you? I hope you are having a nice day. I am reaching out to you for help because I am presently on a search for a leading pharmaceutical/research company that is in need of a Histology Supervisor in the Chicago area. This is a RELIA Exclusive!! My client offers excellent compensation great benefits, relocation assistance and a great group of people to work with. The help I need is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them? Would you possibly be interested in the opportunity? If you or anyone you know might be interested in hearing more about this opportunity Please call me at 866-607-3542 or e-mail me at relia1 at earthlink.net Remember if you refer someone I place You will receive a referral reward bonus. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From criley at dpspa.com Fri Apr 7 05:11:39 2017 From: criley at dpspa.com (Charles Riley) Date: Fri, 7 Apr 2017 06:11:39 -0400 Subject: [Histonet] De-staining IHC slides Message-ID: Is there anyway to destain a cd38 stain in order to use the slide for another stain. We use the leica bond refine detection kits -- Charles Riley HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From CDavis at che-east.org Fri Apr 7 06:38:08 2017 From: CDavis at che-east.org (Cassie P. Davis) Date: Fri, 7 Apr 2017 11:38:08 +0000 Subject: [Histonet] Ventana p16 Message-ID: <4297f17ad3394a15a12f6c4139bed5c2@che-east.org> The Ventana p16 isn't really being discontinued. They just got FDA approval for it, this approval is using the Optiview DAB kit only. They are changing the catalogue # for the lots since the approval . Your sales rep. should provide information if your are getting it from them. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From mpence at grhs.net Fri Apr 7 07:31:09 2017 From: mpence at grhs.net (Mike Pence) Date: Fri, 7 Apr 2017 12:31:09 +0000 Subject: [Histonet] Ventana PD-L1 Message-ID: Is anyone using PD-L1 on the Ventana system? And if so, what CPT codes are you billing for this marker? Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 mpence at grhs.net | www.greatrivermedical.org. www.Facebook.com/GreatRiverHealthSystems | www.Twitter/GreatRiverMed Information in this communication, including attachments, is confidential and intended only for the addressee(s). This communication may contain privileged, confidential, proprietary or trade secret information entitled to protection or exemption from disclosure under law. If you are not an intended recipient, please know that any use, distribution or copying of this communication, or any action taken based on the information in this communication, is unauthorized and may be unlawful. If you received this communication in error, please notify the sender and delete this communication from your device. From alliebecker08 at gmail.com Fri Apr 7 09:30:20 2017 From: alliebecker08 at gmail.com (Allie Becker) Date: Fri, 7 Apr 2017 09:30:20 -0500 Subject: [Histonet] p16 from Ventana In-Reply-To: References: Message-ID: <9AA7B961-02CC-4511-85DB-EDFA47918E8F@gmail.com> Does anybody have more information on what these changes are for the Roche p16? Sent from my iPhone > On Apr 5, 2017, at 16:07, Lynch, Kenia via Histonet wrote: > > Hi all, > > Roche will still have the p16 antibody, but there are a few changes taking > place. Please reach out to your account manager for clarification. > > Thanks, > KCL > > On Wed, Apr 5, 2017 at 3:47 PM, Teri Johnson via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Wait...what? When is Ventana p16 being discontinued? >> >> Teri Johnson, HT(ASCP)QIHC >> Manager, Clinical Trial Testing >> >> T +1 760 516 5954 >> tejohnson at genoptix.com >> >> Navigate BioPharma, Inc. >> A Novartis Company >> 1890 Rutherford Rd. >> Carlsbad, CA 92008 >> USA >> >> Date: Wed, 5 Apr 2017 01:54:06 +0000 >> From: Samantha Claypool >> To: "histonet at lists.utsouthwestern.edu" >> >> Subject: [Histonet] Sigma p16 >> >> Hello Histonet! Anyone out there using (or getting ready to use) the >> p16(ink4a) antibody available from Sigma? We currently use Ventana's but >> will probably buy from Sigma once the Ventana product is discontinued. >> Would love to hear from anyone with experience with Sigma. >> >> Thanks, >> Samantha >> Huyer Laboratory Services >> >> >> >> >> ________________________________ >> >> CONFIDENTIALITY NOTICE: The sender of this email is not an employee or >> agent of, or a contractor or consultant to, Genoptix. This email has been >> sent through the Genoptix email system as a transitional service provided >> by Genoptix, Inc. (?Genoptix?) to Navigate BioPharma Services, Inc. The >> sender of this email is not authorized to represent or to speak on behalf >> of any member of Genoptix, nor to enter into any undertaking, arrangement >> or agreement on behalf of any member of Genoptix. Any such undertaking, >> arrangement or agreement shall not be binding on Genoptix. Genoptix accepts >> no liability for the content of this email, or for the consequences of any >> acts or omissions taken or not taken on the basis of the information >> contained in this email or in any email sent from this email address. All >> reasonable precautions have been taken to ensure no viruses or other >> malware are present in this e-mail and Genoptix cannot accept >> responsibility for any loss or damage arising from the use of this e-mail >> or attachments and you are responsible for ensuring that your own virus >> screening procedures are up to date and fit for purpose. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > *Kenia C. Lynch, MT, HT, QIHC* > > Account Manager, North Carolina > Roche Diagnostics Corporation > 9115 Hague Road, PO Box 50457 > Indianapolis, IN 46250-0457 > > Tel. +1-800-227-2155 > Cell +1-208-972-4088 > kenia.lynch.kl1 at roche.com > > > [image: The Future of H&E] > > > > *Confidentiality Note:* This message is intended only for the use of the > named recipient(s) and may contain confidential and/or proprietary > information. If you are not the intended recipient, please contact the > sender and delete this message. Any unauthorized use of the information > contained in this message is prohibited. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.bernard at comcast.net Sat Apr 8 13:38:31 2017 From: ian.bernard at comcast.net (ian bernard) Date: Sat, 8 Apr 2017 12:38:31 -0600 Subject: [Histonet] Return of Gross Only Specimens for Patient Use Message-ID: <007001d2b097$533a5dd0$f9af1970$@comcast.net> 1. Per a patient request, before returning an amputated digit (toe) to a patient, we will remove the 10 % NBF from the tissue by washing the tissue in running water for how long? 2. As an alternative, we can have the provider submit the gross only toe in saline and then relinquish the toe to the patient, explaining that ultimate degradation of the toe is inevitable unless fixed. Is there another safe fixative alternative that can preserve the toe, say 100% alcohol and for how long? The patient wants to keep his body parts for his burial. 3. We will have the patient sign a document explaining the exposure risk to formalin fixed tissue as well as a release of medical records request form. Your thoughts? 4. Is there any other validated procedure to remove formalin from foreign bodies devices or tissue before release to the patient? Note: The transaction above will take place 2-weeks after the case has been signed out and reported to the referring provider. Your feedback? v/r IB From Richard.Cartun at hhchealth.org Sun Apr 9 09:32:17 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Sun, 9 Apr 2017 14:32:17 +0000 Subject: [Histonet] Return of Gross Only Specimens for Patient Use In-Reply-To: <007001d2b097$533a5dd0$f9af1970$@comcast.net> References: <007001d2b097$533a5dd0$f9af1970$@comcast.net> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E954159AA@HHCEXCHMB03.hhcsystem.org> I strongly discourage patients from taking their tissue out of the hospital. Once the specimen in placed in formalin it becomes a bio-hazard. We will release certain specimens for burial; however, the patient (or family) must contact a funeral home or mortuary to pick-up the specimen, and they must sign a release. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: ian bernard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Saturday, April 08, 2017 2:39 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Return of Gross Only Specimens for Patient Use 1. Per a patient request, before returning an amputated digit (toe) to a patient, we will remove the 10 % NBF from the tissue by washing the tissue in running water for how long? 2. As an alternative, we can have the provider submit the gross only toe in saline and then relinquish the toe to the patient, explaining that ultimate degradation of the toe is inevitable unless fixed. Is there another safe fixative alternative that can preserve the toe, say 100% alcohol and for how long? The patient wants to keep his body parts for his burial. 3. We will have the patient sign a document explaining the exposure risk to formalin fixed tissue as well as a release of medical records request form. Your thoughts? 4. Is there any other validated procedure to remove formalin from foreign bodies devices or tissue before release to the patient? Note: The transaction above will take place 2-weeks after the case has been signed out and reported to the referring provider. Your feedback? v/r IB _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From tbraud at holyredeemer.com Mon Apr 10 09:48:24 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 10 Apr 2017 14:48:24 +0000 Subject: [Histonet] patient taking tissue Message-ID: <48E053DDF6CE074DB6A7414BA05403F812F744@HRHEX03-HOS.holyredeemer.local> Our hospital has a policy against releasing soft tissue to anyone except a funeral home. Realizing that some religions require one to be buried with all their body parts, release to a funeral home meets those requirements without someone finding human remains years down the road, with no record. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Sunday, April 09, 2017 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 161, Issue 8 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Return of Gross Only Specimens for Patient Use (ian bernard) 2. Re: Return of Gross Only Specimens for Patient Use (Cartun, Richard) ---------------------------------------------------------------------- Message: 1 Date: Sat, 8 Apr 2017 12:38:31 -0600 From: "ian bernard" To: Subject: [Histonet] Return of Gross Only Specimens for Patient Use Message-ID: <007001d2b097$533a5dd0$f9af1970$@comcast.net> Content-Type: text/plain; charset="us-ascii" 1. Per a patient request, before returning an amputated digit (toe) to a patient, we will remove the 10 % NBF from the tissue by washing the tissue in running water for how long? 2. As an alternative, we can have the provider submit the gross only toe in saline and then relinquish the toe to the patient, explaining that ultimate degradation of the toe is inevitable unless fixed. Is there another safe fixative alternative that can preserve the toe, say 100% alcohol and for how long? The patient wants to keep his body parts for his burial. 3. We will have the patient sign a document explaining the exposure risk to formalin fixed tissue as well as a release of medical records request form. Your thoughts? 4. Is there any other validated procedure to remove formalin from foreign bodies devices or tissue before release to the patient? Note: The transaction above will take place 2-weeks after the case has been signed out and reported to the referring provider. Your feedback? v/r IB ------------------------------ Message: 2 Date: Sun, 9 Apr 2017 14:32:17 +0000 From: "Cartun, Richard" To: ian bernard Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Return of Gross Only Specimens for Patient Use Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E954159AA at HHCEXCHMB03.hhcsystem.org> Content-Type: text/plain; charset="us-ascii" I strongly discourage patients from taking their tissue out of the hospital. Once the specimen in placed in formalin it becomes a bio-hazard. We will release certain specimens for burial; however, the patient (or family) must contact a funeral home or mortuary to pick-up the specimen, and they must sign a release. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: ian bernard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Saturday, April 08, 2017 2:39 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Return of Gross Only Specimens for Patient Use 1. Per a patient request, before returning an amputated digit (toe) to a patient, we will remove the 10 % NBF from the tissue by washing the tissue in running water for how long? 2. As an alternative, we can have the provider submit the gross only toe in saline and then relinquish the toe to the patient, explaining that ultimate degradation of the toe is inevitable unless fixed. Is there another safe fixative alternative that can preserve the toe, say 100% alcohol and for how long? The patient wants to keep his body parts for his burial. 3. We will have the patient sign a document explaining the exposure risk to formalin fixed tissue as well as a release of medical records request form. Your thoughts? 4. Is there any other validated procedure to remove formalin from foreign bodies devices or tissue before release to the patient? Note: The transaction above will take place 2-weeks after the case has been signed out and reported to the referring provider. Your feedback? v/r IB _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 161, Issue 8 **************************************** From Richard.Cartun at hhchealth.org Mon Apr 10 17:31:08 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 10 Apr 2017 22:31:08 +0000 Subject: [Histonet] IHC for GAB1 and YAP1 Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E95416D89@HHCEXCHMB03.hhcsystem.org> Anyone out there doing IHC testing for GAB1 and YAP1 to subtype medulloblastoma? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Ana.Maluenda at baker.edu.au Tue Apr 11 01:33:51 2017 From: Ana.Maluenda at baker.edu.au (Ana Maluenda) Date: Tue, 11 Apr 2017 06:33:51 +0000 Subject: [Histonet] Anti-fibrin antibody Message-ID: Dear all, I've been trying to optimise an immunohistochemistry protocol for fibrin that has not been working. Was wondering if there is anyone that would have any recommendation of staining protocols for fibrin or recommendation of anti-fibrin antibodies? We are working with cryosections, mouse tissue, embedded fresh (no pre-fixation). I've tried different conditions already (different fixatives, blocking reagents and so on). So far, the antibodies I found commercially available are anti-mouse fibrinogen. This IHC is to complement special stainings (eg Masson and PSR). Thanks in advance. Kind regards, Ana Ana Maluenda Research Assistant Atherothrombosis and Vascular Biology Laboratory Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting privacy at baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg From garethdavisyuma at gmail.com Tue Apr 11 11:30:57 2017 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Tue, 11 Apr 2017 09:30:57 -0700 Subject: [Histonet] No Ribbon Message-ID: I feel like I should be able to figure this one out, but I have been having trouble with getting a ribbon for a while now. I have a Leica Microtome RM2125 and I use either a AccuEdge blade (I get one block to cut then it starts to compress) or a StatCut blade (sections do not stick together at all), both High Profile. The angle is about 4 microns. I change the angle a lot to see if that makes a difference and it doesn't. There are so many factors that could be the issue, I think lack of humidity may be the main one. So, suggestions please!! -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From angie at ka-recruiting.com Tue Apr 11 12:03:44 2017 From: angie at ka-recruiting.com (Angie Laparidis) Date: Tue, 11 Apr 2017 13:03:44 -0400 Subject: [Histonet] NY Histotech Message-ID: Hi Histonetters! Do you know of anyone who has a NYS laboratory license? I have a fantastic private lab client on Long Island (1hr outside of Manhattan). They are looking for someone to fill their Full-Time, permanent, Day-Shift Histotech role! It is a great environment in a great location, and won't be available very long! Please send over an updated resume and let me know the best time to get a hold of you. Sincerely, *Angie Laparidis*Healthcare Recruiter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South, Boston, MA, 02109 W: 617.746-2744 (*please note this is a new number*) F: (617) 507-8009 Angie at ka-recruiting.com Our openings are updated daily at www.ka-recruiting.com From garethdavisyuma at gmail.com Tue Apr 11 12:24:10 2017 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Tue, 11 Apr 2017 10:24:10 -0700 Subject: [Histonet] No Ribbon Message-ID: Yes, I use ice for my blocks and get them as cold as possible. It is a high profile blade holder. The paraffin is good for embedding and processing. I was told to use a low angle for Statcut blades, but I will try it at 10. Thanks -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From paula at excaliburpathology.com Tue Apr 11 12:37:21 2017 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Tue, 11 Apr 2017 17:37:21 +0000 (UTC) Subject: [Histonet] No Ribbon In-Reply-To: References: Message-ID: <2094018006.772606.1491932241842@mail.yahoo.com> Hi, the blades are packed with a tiny, bit of oil. This can cause you to not obtain a ribbon. Try gently cleaning with xylene, followed by alcohol before sectioning.?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Gareth Davis via Histonet To: "Histonet at lists.utsouthwestern.edu" Sent: Tuesday, April 11, 2017 11:55 AM Subject: [Histonet] No Ribbon I feel like I should be able to figure this one out, but I have been having trouble with getting a ribbon for a while now.? I have a Leica Microtome RM2125 and I use either a AccuEdge blade (I get one block to cut then it starts to compress) or a StatCut blade (sections do not stick together at all), both High Profile.? The angle is about 4 microns.? I change the angle a lot to see if that makes a difference and it doesn't.? There are so many factors that could be the issue, I think lack of humidity may be the main one. So, suggestions please!! -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mills at 3scan.com Tue Apr 11 12:58:30 2017 From: mills at 3scan.com (Caroline Miller) Date: Tue, 11 Apr 2017 10:58:30 -0700 Subject: [Histonet] No Ribbon In-Reply-To: <2094018006.772606.1491932241842@mail.yahoo.com> References: <2094018006.772606.1491932241842@mail.yahoo.com> Message-ID: Yes, +1 to Paula, also: - I often found they are too sharp (!!) to ribbon so a gentle wipe (in the direction of the bevel) with a kimwipe helps - Also 'huffing' slightly on the block will warm the paraffin and both smooth out the wrinkles as well as make the sections stick together :) mills On Tue, Apr 11, 2017 at 10:37 AM, Paula Keene Pierce via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi, > the blades are packed with a tiny, bit of oil. This can cause you to not > obtain a ribbon. > Try gently cleaning with xylene, followed by alcohol before > sectioning. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur > Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX > 405-759-7513www.excaliburpathology.com > > From: Gareth Davis via Histonet > To: "Histonet at lists.utsouthwestern.edu" utsouthwestern.edu> > Sent: Tuesday, April 11, 2017 11:55 AM > Subject: [Histonet] No Ribbon > > I feel like I should be able to figure this one out, but I have been having > trouble with getting a ribbon for a while now. I have a Leica Microtome > RM2125 and I use either a AccuEdge blade (I get one block to cut then it > starts to compress) or a StatCut blade (sections do not stick together at > all), both High Profile. The angle is about 4 microns. I change the angle > a lot to see if that makes a difference and it doesn't. There are so many > factors that could be the issue, I think lack of humidity may be the main > one. So, suggestions please!! > > -- > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm > Yuma Gastroenterology > Yuma, AZ 85364 > 928-248-5259 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From liz at premierlab.com Tue Apr 11 13:13:50 2017 From: liz at premierlab.com (Elizabeth Chlipala) Date: Tue, 11 Apr 2017 12:13:50 -0600 Subject: [Histonet] No Ribbon In-Reply-To: References: <2094018006.772606.1491932241842@mail.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE0302C5C0C479@SBS2K8.premierlab.local> I might get some individuals upset but we really need to watch what we do in the histology lab and how that effects the precision of our process. When you breath on the block you are basically generating a thicker section, it's not a good practice to keep. Trust me I used to do this in the past. Thicker sections especially for IHC will generate a more intense stain, most individuals may think that that should not be an issue but it is - in the clinical setting especially when you are staining for therapeutic markers such as Her2 and PD-L1 - 510K cleared kits for these recommend specific section thickness settings and in the research setting if you are running any type of analysis on a batch of samples. If your microtome is set at 4 microns and you are breathing on the block you are not cutting at 4 microns, you are cutting thicker than that and a thicker section will increase staining intensity and therefore alter the results. Just my two cents. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Caroline Miller via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, April 11, 2017 11:59 AM To: Paula Keene Pierce Cc: Histonet Histonet Subject: Re: [Histonet] No Ribbon Yes, +1 to Paula, also: - I often found they are too sharp (!!) to ribbon so a gentle wipe (in the direction of the bevel) with a kimwipe helps - Also 'huffing' slightly on the block will warm the paraffin and both smooth out the wrinkles as well as make the sections stick together :) mills On Tue, Apr 11, 2017 at 10:37 AM, Paula Keene Pierce via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi, > the blades are packed with a tiny, bit of oil. This can cause you to > not obtain a ribbon. > Try gently cleaning with xylene, followed by alcohol before > sectioning. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur > Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH > 405-759-3953FAX 405-759-7513www.excaliburpathology.com > > From: Gareth Davis via Histonet > > To: "Histonet at lists.utsouthwestern.edu" utsouthwestern.edu> > Sent: Tuesday, April 11, 2017 11:55 AM > Subject: [Histonet] No Ribbon > > I feel like I should be able to figure this one out, but I have been > having trouble with getting a ribbon for a while now. I have a Leica > Microtome > RM2125 and I use either a AccuEdge blade (I get one block to cut then > it starts to compress) or a StatCut blade (sections do not stick > together at all), both High Profile. The angle is about 4 microns. I > change the angle a lot to see if that makes a difference and it > doesn't. There are so many factors that could be the issue, I think > lack of humidity may be the main one. So, suggestions please!! > > -- > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology > Yuma, AZ 85364 > 928-248-5259 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ From mills at 3scan.com Tue Apr 11 13:18:36 2017 From: mills at 3scan.com (Caroline Miller) Date: Tue, 11 Apr 2017 11:18:36 -0700 Subject: [Histonet] No Ribbon In-Reply-To: <14E2C6176416974295479C64A11CB9AE0302C5C0C479@SBS2K8.premierlab.local> References: <2094018006.772606.1491932241842@mail.yahoo.com> <14E2C6176416974295479C64A11CB9AE0302C5C0C479@SBS2K8.premierlab.local> Message-ID: Point taken, thanks Elizabeth. My clinical days are long gone, but that is an important consideration. yours, mills On Tue, Apr 11, 2017 at 11:13 AM, Elizabeth Chlipala wrote: > I might get some individuals upset but we really need to watch what we do > in the histology lab and how that effects the precision of our process. > When you breath on the block you are basically generating a thicker > section, it's not a good practice to keep. Trust me I used to do this in > the past. Thicker sections especially for IHC will generate a more intense > stain, most individuals may think that that should not be an issue but it > is - in the clinical setting especially when you are staining for > therapeutic markers such as Her2 and PD-L1 - 510K cleared kits for these > recommend specific section thickness settings and in the research setting > if you are running any type of analysis on a batch of samples. If your > microtome is set at 4 microns and you are breathing on the block you are > not cutting at 4 microns, you are cutting thicker than that and a thicker > section will increase staining intensity and therefore alter the results. > > Just my two cents. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz at premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: Caroline Miller via Histonet [mailto:histonet at lists. > utsouthwestern.edu] > Sent: Tuesday, April 11, 2017 11:59 AM > To: Paula Keene Pierce > Cc: Histonet Histonet > Subject: Re: [Histonet] No Ribbon > > Yes, +1 to Paula, also: > - I often found they are too sharp (!!) to ribbon so a gentle wipe (in the > direction of the bevel) with a kimwipe helps > - Also 'huffing' slightly on the block will warm the paraffin and both > smooth out the wrinkles as well as make the sections stick together > > :) > > mills > > On Tue, Apr 11, 2017 at 10:37 AM, Paula Keene Pierce via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hi, > > the blades are packed with a tiny, bit of oil. This can cause you to > > not obtain a ribbon. > > Try gently cleaning with xylene, followed by alcohol before > > sectioning. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur > > Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH > > 405-759-3953FAX 405-759-7513www.excaliburpathology.com > > > > From: Gareth Davis via Histonet > > > > To: "Histonet at lists.utsouthwestern.edu" > utsouthwestern.edu> > > Sent: Tuesday, April 11, 2017 11:55 AM > > Subject: [Histonet] No Ribbon > > > > I feel like I should be able to figure this one out, but I have been > > having trouble with getting a ribbon for a while now. I have a Leica > > Microtome > > RM2125 and I use either a AccuEdge blade (I get one block to cut then > > it starts to compress) or a StatCut blade (sections do not stick > > together at all), both High Profile. The angle is about 4 microns. I > > change the angle a lot to see if that makes a difference and it > > doesn't. There are so many factors that could be the issue, I think > > lack of humidity may be the main one. So, suggestions please!! > > > > -- > > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology > > Yuma, AZ 85364 > > 928-248-5259 > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Caroline Miller (mills) > Director of Histology > 3Scan.com > 415 2187297 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > This email has been scanned by the Symantec Email Security.cloud service. > For more information please visit http://www.symanteccloud.com > ______________________________________________________________________ > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From Nancy_Schmitt at pa-ucl.com Wed Apr 12 06:35:21 2017 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Wed, 12 Apr 2017 11:35:21 +0000 Subject: [Histonet] egg albumin Message-ID: <0e62f8d9451a4eb98b965f31d6a41e20@mercury.wad.pa-ucl.com> Good Morning- Thoughts on shelf life of egg albumin? Thank you Nancy Schmitt MLT, HT(ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From BDeBrosse-Serra at ionisph.com Wed Apr 12 09:00:33 2017 From: BDeBrosse-Serra at ionisph.com (Bea DeBrosse-Serra) Date: Wed, 12 Apr 2017 14:00:33 +0000 Subject: [Histonet] Histology temp position available at Ionis Pharmaceuticals in Carlsbad, CA Message-ID: We will soon have a temp position available in our very busy histology laboratory at Ionis Pharmaceuticals. If you are interested, please forward your CV to me. Have a great day, Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Ionis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct Carlsbad, CA 92010 760-603-2371 From tmcampbe at fmh.org Wed Apr 12 09:48:41 2017 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Wed, 12 Apr 2017 14:48:41 +0000 Subject: [Histonet] Shurwave Processor Message-ID: Hello all, Is there anyone that can help me with my Shurwave Microwave Processor? The unit is very slow reaching temp. I have to double the times of all steps for it to get up to temperature. I have replaced both magnetrons, filament transformers, high voltage transformers, capacitors, solid state relays, temperature probe (with calibration), and the relay board. It is still not working right. I am set at 100% power. I don't know what else to do besides get a new one but it seems like there should be a fix for this one. Its working. Its just not working like it should. Thanks in advance! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 From relia1 at earthlink.net Wed Apr 12 10:32:04 2017 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 12 Apr 2017 11:32:04 -0400 Subject: [Histonet] Happy Easter and Happy Passover to You, Your family and Friends. And here are some exciting NEW Opportunities in IL, WA, CA, TN, TX and NJ! Message-ID: <000401d2b3a1$f17997b0$d46cc710$@earthlink.net> Hello Histonetters, Happy Easter and Happy Passover to you, your family and Friends. I hope you are enjoying some beautiful spring weather and are getting ready for a lovely Easter Weekend.? (And if you are on day 2 of Matzo Happy Passover and I feel you!)? I have a couple of new opportunities that I want to share.? Here are the hot jobs I want to share with you! RELIA Spotlight Histology Job!!! Histology Supervisor ? Madison, WI Top notch vendor/supplier of histology consumables needs a histology supervisor with strong personnel management experience.? Here are the rest of my sizzlin? hot jobs!!! ? Histology Tech ? Virginia Beach, VA 15K sign on bonus! ? Histology Supervisor ? Chicago, IL ? Lead Histotech ? Vancouver, WA ? Histo Tech ? Modesto, CA ? Histotechnician ? Kingsport, TN ? IHC Specialist ? Modesto, CA ? Dermpath Histotech ? Longview, TX ? Histology Tech ? San Diego, CA ? Histotechnician ? Cranford, NJ All of my clients offer excellent compensation, benefits and relocation assistance.? These are full time permanent positions with stable secure labs.? My clients are eager to interview and hire for these positions! If any of these opportunities are the right one for you RELIA can make it happen!!! Happy Easter and Happy Passover to you, your family and Friends. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From rjbuesa at yahoo.com Wed Apr 12 11:06:02 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 12 Apr 2017 16:06:02 +0000 (UTC) Subject: [Histonet] egg albumin In-Reply-To: <0e62f8d9451a4eb98b965f31d6a41e20@mercury.wad.pa-ucl.com> References: <0e62f8d9451a4eb98b965f31d6a41e20@mercury.wad.pa-ucl.com> Message-ID: <119541487.520013.1492013162301@mail.yahoo.com> Do you have a bottle of "Eggnog"? Egg-albumin will have similar shelf life if refrigerated.Ren? On Wednesday, April 12, 2017 7:40 AM, Nancy Schmitt via Histonet wrote: Good Morning- Thoughts on shelf life of egg albumin? Thank you Nancy Schmitt MLT, HT(ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald at mtsac.edu Wed Apr 12 11:32:05 2017 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Wed, 12 Apr 2017 09:32:05 -0700 Subject: [Histonet] egg albumin In-Reply-To: <119541487.520013.1492013162301@mail.yahoo.com> References: <0e62f8d9451a4eb98b965f31d6a41e20@mercury.wad.pa-ucl.com> <119541487.520013.1492013162301@mail.yahoo.com> Message-ID: I respectively disagree with this analogy. Eggnog is dairy based, made with milk, heavy cream and sugar. Dairy products have a much shorter life than egg whites. From: Rene J Buesa via Histonet To: Nancy Schmitt , "'histonet at lists.utsouthwestern.edu'" Date: 04/12/2017 09:07 AM Subject: Re: [Histonet] egg albumin Do you have a bottle of "Eggnog"? Egg-albumin will have similar shelf life if refrigerated.Ren? On Wednesday, April 12, 2017 7:40 AM, Nancy Schmitt via Histonet wrote: Good Morning- Thoughts on shelf life of egg albumin? Thank you Nancy Schmitt MLT, HT(ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tilycyn at auburn.edu Wed Apr 12 14:03:01 2017 From: tilycyn at auburn.edu (Cynthia Hutchinson) Date: Wed, 12 Apr 2017 19:03:01 +0000 Subject: [Histonet] Looking for inverted microscope Message-ID: <4310dd06ccac471895790528d9f8e67b@ex0.auburn.edu> Good afternoon, I am looking for an inverted microscope (used, refurbs acceptable) for a new investigator with limited resources. Vendors please feel free to contact me off list. Kind regards, Cindy Cynthia Tily Hutchinson Rsch Asst IV Pathobiology 166 Greene Hall Coll of Vet Med Auburn Univ 36849 (334)844 7020 From jaylundgren at gmail.com Wed Apr 12 14:29:57 2017 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 12 Apr 2017 15:29:57 -0400 Subject: [Histonet] Looking for inverted microscope In-Reply-To: <4310dd06ccac471895790528d9f8e67b@ex0.auburn.edu> References: <4310dd06ccac471895790528d9f8e67b@ex0.auburn.edu> Message-ID: Have you tried obtaining one from an Australian pathology lab? On Wed, Apr 12, 2017 at 3:03 PM, Cynthia Hutchinson via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good afternoon, > I am looking for an inverted microscope (used, refurbs acceptable) for a > new investigator with limited resources. > Vendors please feel free to contact me off list. > Kind regards, > Cindy > > Cynthia Tily Hutchinson > Rsch Asst IV > Pathobiology > 166 Greene Hall > Coll of Vet Med > Auburn Univ 36849 > (334)844 7020 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Blanca.Lopez at UTSouthwestern.edu Thu Apr 13 08:09:32 2017 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Thu, 13 Apr 2017 13:09:32 +0000 Subject: [Histonet] help!! Message-ID: <4d5edeb9eb2c4d768108c8932ad3fd57@SWMS13MAIL12.swmed.org> Hello! I just need a help with a simple question...Is anyone can explain me what is the purpose between performing immunohistochemistry and Immunofluorescence? Thanks :) Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From HornHV at archildrens.org Thu Apr 13 08:17:33 2017 From: HornHV at archildrens.org (Horn, Hazel V) Date: Thu, 13 Apr 2017 13:17:33 +0000 Subject: [Histonet] orders Message-ID: In the 25+ years I have worked at ACH we have never allowed a resident physician to order a pathology test. I am now being asked why we do this? I have no idea if there is a rule from Medicaid or insurance providers that all orders must be from a staff physician. Does your hospital allow residents to place pathology orders? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children?s Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org From LRaff at uropartners.com Thu Apr 13 09:36:44 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 13 Apr 2017 14:36:44 +0000 Subject: [Histonet] Testicular biopsy processing Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF1134A0C0@COLOEXCH01.uropartners.local> Hello all-although we are a Uropath lab, up until now we have not processed testicular biopsies, and it has been years since I have done so. Do most labs still use Bouin's Solution as the fixative of choice? Any precautions or special handling techniques? Thanks for any and all input. ------ Lester J. Raff, MD MBA UroPartners Latest blog: http://www.chicagonow.com/downsize-maybe/2017/04/learning-to-be-a-patient/ From mills at 3scan.com Thu Apr 13 10:44:16 2017 From: mills at 3scan.com (Caroline Miller) Date: Thu, 13 Apr 2017 08:44:16 -0700 Subject: [Histonet] help!! In-Reply-To: <4d5edeb9eb2c4d768108c8932ad3fd57@SWMS13MAIL12.swmed.org> References: <4d5edeb9eb2c4d768108c8932ad3fd57@SWMS13MAIL12.swmed.org> Message-ID: Blanca, Here are my feelings on this, and I am sure a lot of other folks have feels here too, so please chime in. 1 - I feel that most clinical labs are more on the IHC bandwagon and research labs are IF (with the exception of IgG staining in kidney biopsies or bullous disease in skin- which is because the antibodies don't like formalin fixing (if this is now wrong I am sorry, I haven't been in a clinical lab in quite a while). Research labs are often also working with genetically encoded fluorophores such as GFP, YFP, mCherry 2 - Formalin fixation (especially over fixation) can often lead to a large amount of autofluorescence in the 488 region, which is a common place for secondary antibodies and also GFP. Research labs have a lot more control over their fixation protocols. 3 - The microscopes commonly available to clinical labs are bright field scopes and in research labs fluorescent scopes 4 - Fluorescence can provide more contrast to a positively localized fluorophore, but sometimes at the detriment of viewing the overall morphology of the tissue like you get with bright field IHC and a nuclear counterstain. 5 - Research lab protocols are often very 'experimental' and can lead to increased tissue damage, which again is not viewed under the fluorescence microscope (as much). Clinical labs have lots of experience and also defined protocols that work well in the IHC / bright field space. 6 - the only real difference is the detection method, you can use any primary antibody with either ABC/ impress / enzyme based methods or with fluorophore conjugated secondaries. So, in short - no *real* reason, but mainly that is the way things shook out. I could go on about researchers not understanding how to take photos on a bright field scopes too, but that is too broad a statement, but as a core director I saw them being more comfortable with the fluorescent methods :) mills On Thu, Apr 13, 2017 at 6:09 AM, Blanca Lopez via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello! > I just need a help with a simple question...Is anyone can explain me what > is the purpose between performing immunohistochemistry and > Immunofluorescence? > Thanks :) > > Blanca Lopez > Histotech (ASCP) > UTSW Tissue Resource K1.210 > Simmons Comprehensive Cancer Center > UT Southwestern Medical Center > Telephone: 214-648-7598 > Email: Blanca.Lopez at utsouthwestern.edu > > > ________________________________ > > UT Southwestern > > > Medical Center > > > > The future of medicine, today. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From Timothy.Morken at ucsf.edu Thu Apr 13 10:45:27 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 13 Apr 2017 15:45:27 +0000 Subject: [Histonet] help!! In-Reply-To: <4d5edeb9eb2c4d768108c8932ad3fd57@SWMS13MAIL12.swmed.org> References: <4d5edeb9eb2c4d768108c8932ad3fd57@SWMS13MAIL12.swmed.org> Message-ID: <761E2B5697F795489C8710BCC72141FF8E5CAF6F@ex07.net.ucsf.edu> Blanca, immunofluorescence (IF) is a subset of immunochemistry. Immunohistochemistry is also a subset of immunochemistry. There is some overlap between the two. Immunohistochemistry denotes immunochemistry done on tissue sections ("-histo-" =" tissue"). But we can also use other enzymes to label the antibodies for immunohistochemistry (peroxidase, alkaline phosphatase, etc). IF is just one of many methods of labeling the antibodies with a visual label. Others are peroxidase and alkaline phosphatase. Generally IF is done on "fresh" cells or tissue. For tissue it is normally frozen tissue. IF can be done on cells (ie, immunocytochemistry) either on slides (smears, various preparations) or in solution as with flow cytometry - the cells are labeled with fluorescent-labeled antibodies and sorted by color (or no color). Generally the IF method is faster to perform because there is no processing beyond freezing the tissue. In the past IF was also more sensitive due to dark field microscopy in the fluorescence microscope. With the advent of various methods to amplify the signal (avidin -biotin, polymers with multiple enzymes) the peroxidase methods are just as sensitive, if not more so. But fresh or frozen tissue has the advantage of the epitopes remaining unfixed, especially by formalin - which can mask the antigen from the antibody. Some antibodies do not work well on formalin-fixed tissue, even if antigen retrieval is used, so frozen tissue or cells are the best option. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, April 13, 2017 6:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] help!! Hello! I just need a help with a simple question...Is anyone can explain me what is the purpose between performing immunohistochemistry and Immunofluorescence? Thanks :) Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV at archildrens.org Thu Apr 13 10:56:39 2017 From: HornHV at archildrens.org (Horn, Hazel V) Date: Thu, 13 Apr 2017 15:56:39 +0000 Subject: [Histonet] Fw: orders from resident surgeons In-Reply-To: References: Message-ID: I was not clear enough in my question. I'm not speaking of pathology residents ordering tests. I'm asking if you allow general resident physicians to order pathology tests. Such as gross and micro. We are a teaching hospital and have never allowed a resident to order pathology if they aren't a staff physician (surgeon). Thanks! ________________________________ From: Horn, Hazel V Sent: Thursday, April 13, 2017 8:17 AM To: histonet Subject: orders In the 25+ years I have worked at ACH we have never allowed a resident physician to order a pathology test. I am now being asked why we do this? I have no idea if there is a rule from Medicaid or insurance providers that all orders must be from a staff physician. Does your hospital allow residents to place pathology orders? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children?s Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org From relia1 at earthlink.net Thu Apr 13 11:04:17 2017 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 13 Apr 2017 12:04:17 -0400 Subject: [Histonet] RELIA Special Histology Alert - Strike While the Iron's Hot! and Advance in leadership!! 4-13-2017 Message-ID: <112301d2b46f$9abb4760$d031d620$@earthlink.net> Hi Histonetters, How are you? I hope you are having a great week!! Are you ready for the next step in your career? Histo tech to lead tech or Lead Tech to Supervisor?? Supervisor to Manager? These days you have to strike when the iron?s HOT! I am working with several of my best clients who are offering the rare and unique opportunity to STEP UP! Are you someone who: 1. Wants to move into or up in management 2. Doesn?t see a path to management in your current job 3. Has the degrees, experience and certifications and is looking for the right opportunity I am currently working with several top employers who want to hire someone to take that next step into a lead tech or supervisor or manager role. These opportunities don?t come along every day! Here are the locations: WI WA CA VA My clients offer excellent compensation, benefits, relocation assistance and environments that are great to work in. These are permanent full time positions in busy growing private labs. The help I need is do you know anyone that might be interested in hearing about any of these opportunities? If so could you please forward my e-mail to them? If you are interested in any of these positions please call me ASAP at 866-607-3542 or on my cell/text at 407-353-5070 or e-mail me at relia1 at earthlink.net Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From rjbuesa at yahoo.com Thu Apr 13 11:23:28 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 13 Apr 2017 16:23:28 +0000 (UTC) Subject: [Histonet] Fw: orders from resident surgeons In-Reply-To: References: Message-ID: <2019913216.1400242.1492100608813@mail.yahoo.com> At my hospital we accepted work orders from our pathologists only. If a physician resident wanted some test, it has to be approved by our pathologist.Ren? On Thursday, April 13, 2017 12:18 PM, "Horn, Hazel V via Histonet" wrote: I was not clear enough in my question.? I'm not speaking of pathology residents ordering tests.? I'm asking if you allow general resident physicians to order pathology tests.? Such as gross and micro. We are a teaching hospital and have never allowed a resident to order pathology if they aren't a staff physician (surgeon). Thanks! ________________________________ From: Horn, Hazel V Sent: Thursday, April 13, 2017 8:17 AM To: histonet Subject: orders In the 25+ years I have worked at ACH we have never allowed a resident physician to order a pathology test.? I am now being asked why we do this?? I have no idea if there is a rule from Medicaid or insurance providers that all orders must be from a staff physician.? Does your hospital allow residents to place pathology orders? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children?s Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alamberth at lji.org Thu Apr 13 13:33:56 2017 From: alamberth at lji.org (Angela Lamberth) Date: Thu, 13 Apr 2017 11:33:56 -0700 Subject: [Histonet] Fabric softener to "decalcify" bone? Message-ID: Hi netters! A friend sent me the link below and we are curious to hear your thoughts and experiences with using fabric softener (in lieu of standard decal methods) on bone before processing. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309838/ Best, Angela -- Angela Lamberth Histology Technician III Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 From alamberth at lji.org Thu Apr 13 19:19:16 2017 From: alamberth at lji.org (Angela Lamberth) Date: Thu, 13 Apr 2017 17:19:16 -0700 Subject: [Histonet] Fabric softener to "decalcify" bone? - Downy has formic acid?? Message-ID: Just googled the ingredients and found this: https://imgur.com/a/Y5170 On Thursday, April 13, 2017, Angela Lamberth wrote: > Hi netters! > > A friend sent me the link below and we are curious to hear your thoughts > and experiences with using fabric softener (in lieu of standard decal > methods) on bone before processing. > > https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309838/ > > Best, > Angela > > -- > Angela Lamberth > Histology Technician III > Histology Core Lab > La Jolla Institute for Allergy & Immunology > 9420 Athena Circle > La Jolla, CA 92037 > -- Angela Lamberth Histology Technician III Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 From TNMayer at mdanderson.org Fri Apr 14 12:39:43 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Fri, 14 Apr 2017 17:39:43 +0000 Subject: [Histonet] Fabric softener to "decalcify" bone? (Angela Lamberth) Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8839EB2DFB@D1PWPEXMBX08.mdanderson.edu> I have used fabric softener to surface soften nails. It worked ok, but took a loooong time. Quality was pretty good. Toysha Mayer Message: 1 Date: Thu, 13 Apr 2017 11:33:56 -0700 From: Angela Lamberth To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fabric softener to "decalcify" bone? Message-ID: Content-Type: text/plain; charset=UTF-8 Hi netters! A friend sent me the link below and we are curious to hear your thoughts and experiences with using fabric softener (in lieu of standard decal methods) on bone before processing. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5309838/ Best, Angela -- Angela Lamberth Histology Technician III Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 ***************************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From Timothy.Morken at ucsf.edu Fri Apr 14 13:44:43 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 14 Apr 2017 18:44:43 +0000 Subject: [Histonet] hirsch-peiffer cresyl violet method? Message-ID: <761E2B5697F795489C8710BCC72141FF8E5CB62F@ex07.net.ucsf.edu> Hi all, Does anyone have the method for Hirsch-peiffer cresyl violet used for metachromatic leukodystrophy? On frozen sections. We are trying to find out if it is different than the usual cresyl violet with acetic acid method for Nissl bodies. I've found many references to it, but none that give the procedure. And the original paper is from 1955 not easily available. And is in German.... Thanks for any help. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From JMacDonald at mtsac.edu Fri Apr 14 23:27:12 2017 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Fri, 14 Apr 2017 21:27:12 -0700 Subject: [Histonet] hirsch-peiffer cresyl violet method? In-Reply-To: <761E2B5697F795489C8710BCC72141FF8E5CB62F@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF8E5CB62F@ex07.net.ucsf.edu> Message-ID: Tim, I have this method in one of medical technology books from many years ago. Lynch's Medical Laboratory Technology II. The author of the Histology section was Charles F. A. Culling. There are two procedures, one for urine and one for nervous system tissue. I assume you want the nervous system? Procedure: 1. Cut 8 to 12 um frozen sections of formalin-fixed tissue into distilled water. 2. Stain free-floating sections in 1% cresyl echt violet in 1% acetic acid (pH about 2.7) for 10 minutes (pH 3.5 to 3.6 is preferable) as with urine. 3. Rinse in distilled water and mount in glycerin. Results Granules of metachromatic leukodystrophy - golden brown that remains uncvhanged after treatment with 1% ammonium water. The granules stain positively with PAS in paraffin sections, but the present author has fuond that the PAS-positive reaction faded in routinely mounted sections after 6 to 12 months. I can scan the page for you if you'd like. Jennifer From: "Morken, Timothy via Histonet" To: Histonet Date: 04/14/2017 11:45 AM Subject: [Histonet] hirsch-peiffer cresyl violet method? Hi all, Does anyone have the method for Hirsch-peiffer cresyl violet used for metachromatic leukodystrophy? On frozen sections. We are trying to find out if it is different than the usual cresyl violet with acetic acid method for Nissl bodies. I've found many references to it, but none that give the procedure. And the original paper is from 1955 not easily available. And is in German.... Thanks for any help. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Sat Apr 15 13:46:21 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 15 Apr 2017 14:46:21 -0400 Subject: [Histonet] Hirsch-Pfeiffer cresyl violet method Message-ID: Tim Morken in pathology at UC San Francisco Medical Center asks about the Hirsch-Pfeiffer (correct spelling) cresyl violet stain for frozen sections. "I've found many references to it, but none that give the procedure. And the original paper is from 1955 not easily available. And is in German." If you can get the paper, I can read it for you. Bob Richmond Samurai Pathologist Maryville TN From Timothy.Morken at ucsf.edu Mon Apr 17 09:46:29 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 17 Apr 2017 14:46:29 +0000 Subject: [Histonet] Hirsch-Pfeiffer cresyl violet method In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF8E5CB8F9@ex07.net.ucsf.edu> Bob, Thanks for the offer! I finally found a copy and Google Translate did a pretty good job on it, and we have a person in the lab who, it turns out, can read german, so I think we are set. As an aside, the "clues" I got from tidbits of the procedure mentioned in other papers turned out to have little basis in reality to the original method described in the paper (concentrations, times, temperatures all different!), so I'm glad I found the original. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Saturday, April 15, 2017 11:46 AM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Hirsch-Pfeiffer cresyl violet method Tim Morken in pathology at UC San Francisco Medical Center asks about the Hirsch-Pfeiffer (correct spelling) cresyl violet stain for frozen sections. "I've found many references to it, but none that give the procedure. And the original paper is from 1955 not easily available. And is in German." If you can get the paper, I can read it for you. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abadesuyi at nrh-ok.com Mon Apr 17 14:17:24 2017 From: abadesuyi at nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Mon, 17 Apr 2017 14:17:24 -0500 Subject: [Histonet] CAP regulations regarding storage of aliquots of concentrated antibodies Message-ID: <04EE4F75BB5FB246ADB68D69B7460443B98877B477@MAIL.nrhnt.nrh-ok.com> Hi, I will like to know the CAP regulations regarding storage of aliquots of concentrated antibodies in refrigerator freezers. Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS, Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 abadesuyi at nrh-ok.com ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From craigak12 at gmail.com Mon Apr 17 14:32:34 2017 From: craigak12 at gmail.com (J B) Date: Mon, 17 Apr 2017 19:32:34 +0000 Subject: [Histonet] Tissue Disposal Procedure: Message-ID: Does anyone have a tissue disposal procedure that you can share? How do you log, track, etc specimens that have been disposed? Thank you, JB -- Have a great day! From sccrshlly at yahoo.com Mon Apr 17 17:48:39 2017 From: sccrshlly at yahoo.com (Shelly Coker) Date: Mon, 17 Apr 2017 22:48:39 +0000 (UTC) Subject: [Histonet] Texas Society for Histotechnology Annual Symposium May 5-7, 2017 References: <308318894.2207434.1492469319941.ref@mail.yahoo.com> Message-ID: <308318894.2207434.1492469319941@mail.yahoo.com> Hello Histonetters! I wanted to share an opportunity to earn some valuable CEU's while networking and enjoying the company of your peers. ?We are having our 39th Annual Texas Society for Histotechnology Symposium ad Convention. ?We have a wide variety of workshops available from basic skills to advanced IHC techniques. ?Join us May 5-7 at the Austin Renaissance and connect with fellow histology professionals. ?Visit the following link for hotel and class information ?The Texas Society of Histotechnology?. | | | The Texas Society of Histotechnology | | | From tony.henwood at health.nsw.gov.au Mon Apr 17 20:03:22 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue, 18 Apr 2017 01:03:22 +0000 Subject: [Histonet] help!! In-Reply-To: <4d5edeb9eb2c4d768108c8932ad3fd57@SWMS13MAIL12.swmed.org> References: <4d5edeb9eb2c4d768108c8932ad3fd57@SWMS13MAIL12.swmed.org> Message-ID: <0237449DE79DBC45B686AB82CDCD16FF9553FFE8@SVDCMBX-MEX008.nswhealth.net> Hi Bianca, Well for most Pathology departments, Immunofluorescence (IF) is used for Renal and Skin biopsies; looking for human Immunoglobulin (Igs) deposition on basement membranes. The advantage here is not so much the fluorescence, but that we use unfixed frozen sections. The buffer rinse before antibody application, removes un-bound serum immunoglobulins, leaving any pathological bound Igs for the IF antibody to bind to. This gives a clean result. If one would do IF on formalin-fixed paraffin sections of renal or skin biopsies, you would find heavy background due to the fixative cross-linking serum Igs to tissue and cells (which would usually be removed by the buffer rinse if unfixed frozen sections were used - see above). IF, apart from being a historic method, also does not suffer from endogenous peroxidase that would need to be blocked if peroxidase was used in place of fluorescence. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 13 April 2017 11:10 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] help!! Hello! I just need a help with a simple question...Is anyone can explain me what is the purpose between performing immunohistochemistry and Immunofluorescence? Thanks :) Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From JMacDonald at mtsac.edu Mon Apr 17 21:51:50 2017 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Mon, 17 Apr 2017 19:51:50 -0700 Subject: [Histonet] CE opportunities Message-ID: The California Society for Histotechnology is hosting their annual symposium May 18-21 in San Jose. Please join us for some great workshops and California spring weather. San Jose is a great airport to fly into. More information can be found at: http://californiahistology.org/events.html The deadline for CSH hotel pricing is April 28. From allanvv at gmail.com Tue Apr 18 02:50:40 2017 From: allanvv at gmail.com (Allan Wang) Date: Tue, 18 Apr 2017 03:50:40 -0400 Subject: [Histonet] help!! In-Reply-To: <0237449DE79DBC45B686AB82CDCD16FF9553FFE8@SVDCMBX-MEX008.nswhealth.net> References: <4d5edeb9eb2c4d768108c8932ad3fd57@SWMS13MAIL12.swmed.org> <0237449DE79DBC45B686AB82CDCD16FF9553FFE8@SVDCMBX-MEX008.nswhealth.net> Message-ID: Tim and Tony, Why couldn't DAB be used on frozen sections in your example? Allan On Mon, Apr 17, 2017 at 9:03 PM, Tony Henwood (SCHN) via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Bianca, > Well for most Pathology departments, Immunofluorescence (IF) is used for > Renal and Skin biopsies; looking for human Immunoglobulin (Igs) deposition > on basement membranes. The advantage here is not so much the fluorescence, > but that we use unfixed frozen sections. The buffer rinse before antibody > application, removes un-bound serum immunoglobulins, leaving any > pathological bound Igs for the IF antibody to bind to. This gives a clean > result. > > If one would do IF on formalin-fixed paraffin sections of renal or skin > biopsies, you would find heavy background due to the fixative cross-linking > serum Igs to tissue and cells (which would usually be removed by the buffer > rinse if unfixed frozen sections were used - see above). > > IF, apart from being a historic method, also does not suffer from > endogenous peroxidase that would need to be blocked if peroxidase was used > in place of fluorescence. > > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, 13 April 2017 11:10 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] help!! > > Hello! > I just need a help with a simple question...Is anyone can explain me what > is the purpose between performing immunohistochemistry and > Immunofluorescence? > Thanks :) > > Blanca Lopez > Histotech (ASCP) > UTSW Tissue Resource K1.210 > Simmons Comprehensive Cancer Center > UT Southwestern Medical Center > Telephone: 214-648-7598 > Email: Blanca.Lopez at utsouthwestern.edu > > > ________________________________ > > UT Southwestern > > > Medical Center > > > > The future of medicine, today. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain > confidential information. If you are not the intended recipient, please > delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and > are not necessarily the views of NSW Health or any of its entities. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From MLashus at pathgroup.com Tue Apr 18 13:55:59 2017 From: MLashus at pathgroup.com (Mighnon Lashus) Date: Tue, 18 Apr 2017 18:55:59 +0000 Subject: [Histonet] PT result forms Message-ID: Does anyone have a PT result form to use when the CAP PT results have not been submitted on time? Would you be willing to share? Thanks, Mighnon Lashus, HT (ASCP) Laboratory Manager PathGroup 4071 S. Access Rd, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus at pathgroup.com Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup immediately at 615-562-9255. Thank you From tony.henwood at health.nsw.gov.au Tue Apr 18 19:18:40 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 19 Apr 2017 00:18:40 +0000 Subject: [Histonet] help!! In-Reply-To: References: <4d5edeb9eb2c4d768108c8932ad3fd57@SWMS13MAIL12.swmed.org> <0237449DE79DBC45B686AB82CDCD16FF9553FFE8@SVDCMBX-MEX008.nswhealth.net> Message-ID: <0237449DE79DBC45B686AB82CDCD16FF9554061E@SVDCMBX-MEX008.nswhealth.net> You can use DAB but the issue of endogenous peroxidase will rear its ugly head. And I suppose IF being around since 1955, when Mellors first applied the technique to renal tissue, it has a long history of diagnostic application that is hard to replace. There are other enzymes that could be used that, not being present in human tissue, would not require endogenous enzyme blocking for example glucose oxidase. The advantage of this enzyme is that there is no endogenous glucose oxidase activity in mammalian tissues. Weening, J. J., & Jennette, J. C. (2012). Historical milestones in renal pathology. Virchows Archiv, 461(1), 3-11. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From: Allan Wang [mailto:allanvv at gmail.com] Sent: Tuesday, 18 April 2017 5:51 PM To: Tony Henwood (SCHN) Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] help!! Tim and Tony, Why couldn't DAB be used on frozen sections in your example? Allan On Mon, Apr 17, 2017 at 9:03 PM, Tony Henwood (SCHN) via Histonet > wrote: Hi Bianca, Well for most Pathology departments, Immunofluorescence (IF) is used for Renal and Skin biopsies; looking for human Immunoglobulin (Igs) deposition on basement membranes. The advantage here is not so much the fluorescence, but that we use unfixed frozen sections. The buffer rinse before antibody application, removes un-bound serum immunoglobulins, leaving any pathological bound Igs for the IF antibody to bind to. This gives a clean result. If one would do IF on formalin-fixed paraffin sections of renal or skin biopsies, you would find heavy background due to the fixative cross-linking serum Igs to tissue and cells (which would usually be removed by the buffer rinse if unfixed frozen sections were used - see above). IF, apart from being a historic method, also does not suffer from endogenous peroxidase that would need to be blocked if peroxidase was used in place of fluorescence. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 13 April 2017 11:10 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] help!! Hello! I just need a help with a simple question...Is anyone can explain me what is the purpose between performing immunohistochemistry and Immunofluorescence? Thanks :) Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From lmarie08 at uga.edu Wed Apr 19 07:43:51 2017 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Wed, 19 Apr 2017 12:43:51 +0000 Subject: [Histonet] processor died overnight Message-ID: Hello Histoworld, I came in this morning to find that the processor died halfway through process last night. The tissues are in 100% ETOH exactly half point. We do have a back- up processor. In your professional experiences, would these tissues be salvageable? Could I create a new program on the backup processor that finishes the process from that point and transfer the tissues over? Thanks. From Julia.Cates at AHSS.ORG Wed Apr 19 08:11:06 2017 From: Julia.Cates at AHSS.ORG (Cates, Julia) Date: Wed, 19 Apr 2017 13:11:06 +0000 Subject: [Histonet] A moment of thanks Message-ID: Good Morning Histonet! I wanted to take a moment to express my gratitude to all of my colleagues on histonet. You are an invaluable resource in a world that is spread-out and I treasure the ability to reach out to you when I have a problem. Case and point, I have been struggling with a H&E problem for months. When looking at the slide, you would think the problem was a cutting artifact. I had a service technician go over the microtome multiple times with minimal resolution. The second part of the problem is that the issue was intermittent. Anyone troubleshooting knows how confounding this can be! After exhausting all cutting possibilities, I started looking at the stainer. That is where histonet came on to the scene. I searched hoping to find someone who had had the same problem or similar and sure enough I found it. One response from Tim Morken has solved my issue and I can't thank you enough. I will say that this is also an important lesson for me as well. I should have reached out sooner for help. I wouldn't have had to struggle or put my pathologist through looking at garbage slides. Lesson learned. Anyway, my thanks again to you all! Thanks, Julia Cates, HT(ASCP)cm This message (including any attachments) is intended only for the use of the individual or entity to which it is addressed and may contain information that is non-public, proprietary, privileged, confidential, and exempt from disclosure under applicable law or may constitute as attorney work product. If you are not the intended recipient, you are hereby notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, notify us immediately by telephone and (i) destroy this message if a facsimile or (ii) delete this message immediately if this is an electronic communication. Thank you. From rjbuesa at yahoo.com Wed Apr 19 08:41:47 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 19 Apr 2017 13:41:47 +0000 (UTC) Subject: [Histonet] processor died overnight In-Reply-To: References: Message-ID: <174147213.3806623.1492609307572@mail.yahoo.com> In 100% EthOL the tissues are completely "salvaged" and you can prepare the program to continue the steps until melted paraffin.If there are delicate tissue perhaps they will be "over-dried" but that is easily "compensated" during microtomy.Ren? On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet wrote: Hello Histoworld, I came in this morning to find that the processor died halfway through process last night. The tissues are in 100% ETOH exactly half point. We do have a back- up processor. In your professional experiences, would these tissues be salvageable? Could I create a new program on the backup processor that finishes the process from that point and transfer the tissues over? Thanks. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Wed Apr 19 11:11:51 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 19 Apr 2017 16:11:51 +0000 Subject: [Histonet] IF vs IHC another reason Message-ID: <48E053DDF6CE074DB6A7414BA05403F8130EAF@HRHEX03-HOS.holyredeemer.local> Another reason that IHC is used instead of IF is with IHC, one preserves the ability to see tissue/cell morphology through Light Microscopy at the same time as the visual IHC label. Morphology is difficult to see with IF, with the exception of the fluorescein labeled area. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 13 April 2017 11:10 PM Hello! I just need a help with a simple question...Is anyone can explain me what is the purpose between performing immunohistochemistry and Immunofluorescence? Thanks :) Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu From mills at 3scan.com Wed Apr 19 11:17:24 2017 From: mills at 3scan.com (Caroline Miller) Date: Wed, 19 Apr 2017 09:17:24 -0700 Subject: [Histonet] processor died overnight In-Reply-To: <174147213.3806623.1492609307572@mail.yahoo.com> References: <174147213.3806623.1492609307572@mail.yahoo.com> Message-ID: <6156911D-EDE8-4C77-9C6D-36BA64BAF03F@3scan.com> Yes, totally +1 to Rene, they should be fine. (That has totally happened to me too)! Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 > On Apr 19, 2017, at 6:41 AM, Rene J Buesa via Histonet wrote: > > In 100% EthOL the tissues are completely "salvaged" and you can prepare the program to continue the steps until melted paraffin.If there are delicate tissue perhaps they will be "over-dried" but that is easily "compensated" during microtomy.Ren? > > On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet wrote: > > > Hello Histoworld, > > I came in this morning to find that the processor died halfway through process last night. The tissues are in 100% ETOH exactly half point. We do have a back- up processor. In your professional experiences, would these tissues be salvageable? Could I create a new program on the backup processor that finishes the process from that point and transfer the tissues over? > > Thanks. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Angela.McNabola at bpthosp.org Wed Apr 19 11:26:56 2017 From: Angela.McNabola at bpthosp.org (McNabola, Angela) Date: Wed, 19 Apr 2017 16:26:56 +0000 Subject: [Histonet] processor died overnight In-Reply-To: <6156911D-EDE8-4C77-9C6D-36BA64BAF03F@3scan.com> References: <174147213.3806623.1492609307572@mail.yahoo.com> <6156911D-EDE8-4C77-9C6D-36BA64BAF03F@3scan.com> Message-ID: <69AA9D996B56BF4EBCBB8EA666461B9326EBBB32@mbx9vp.YNHH.ORG> It has happened to us as well. I'm not sure where you work, but our processors have alarms that go to security. So I have gotten a call on off hours to come in and fix the problem or try to trouble shoot. Some of the newer processors can be linked to your phone, etc. -----Original Message----- From: Caroline Miller via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 19, 2017 12:17 PM To: Rene J Buesa Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] processor died overnight Yes, totally +1 to Rene, they should be fine. (That has totally happened to me too)! Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 > On Apr 19, 2017, at 6:41 AM, Rene J Buesa via Histonet wrote: > > In 100% EthOL the tissues are completely "salvaged" and you can prepare the program to continue the steps until melted paraffin.If there are delicate tissue perhaps they will be "over-dried" but that is easily "compensated" during microtomy.Ren? > > On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet wrote: > > > Hello Histoworld, > > I came in this morning to find that the processor died halfway through process last night. The tissues are in 100% ETOH exactly half point. We do have a back- up processor. In your professional experiences, would these tissues be salvageable? Could I create a new program on the backup processor that finishes the process from that point and transfer the tissues over? > > Thanks. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message originates from the Yale New Haven Health System. The information contained in this message may be privileged and confidential. If you are the intended recipient you must maintain this message in a secure and confidential manner. If you are not the intended recipient, please notify the sender immediately and destroy this message. Thank you. From criley at dpspa.com Wed Apr 19 11:31:10 2017 From: criley at dpspa.com (Charles Riley) Date: Wed, 19 Apr 2017 12:31:10 -0400 Subject: [Histonet] Cytology pap staining Message-ID: I have been put in charge of figuring out why our pap stains are light on the hematoxylin. Everything was filtered and used fresh as per usual using the same protocol we have used for the past two years. If anyone has any suggestions as to how to fix this problem I would greatly appreciate it. If you need more information please let me know and I will get it for you to help assist you in assisting me -- Charles Riley HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From mneglia at trajanscimed.com Wed Apr 19 11:36:00 2017 From: mneglia at trajanscimed.com (Marc Neglia) Date: Thu, 20 Apr 2017 02:36:00 +1000 Subject: [Histonet] Pathology slide survey - AMAZON GIFT CARD OFFER In-Reply-To: <61E7E1B920537D4BABCCDF8D8B6E70D1062B2A36E464@mifune1> References: <61E7E1B920537D4BABCCDF8D8B6E70D1062B2A2FD9E3@mifune1> <61E7E1B920537D4BABCCDF8D8B6E70D1062B2A36E464@mifune1> Message-ID: <61E7E1B920537D4BABCCDF8D8B6E70D1062B2A36EA54@mifune1> REMINDER: Survey will close at the end of this month. Respond now to be entered into the drawing. Hello Histonetters, I am conducting a study to learn about your experiences in the laboratory with microscope slides. The information gathered will help assist with product development decisions that are aimed at improving patient outcomes and optimizing your pathology workflow. To participate in this short, 5 minute survey, please click on the link below: https://www.surveymonkey.com/r/BKNBBWV As a token of appreciation, we will randomly select four (4) respondents to receive a $50 Amazon gift card after the survey has been completed. I would like to thank you in advance for your participation, and I look forward to viewing your responses. Best regards, Marc Marc Neglia Trajan Scientific and Medical mneglia at trajanscimed.com www.trajanscimed.com _______________________________________________ Histonet mailing list http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Wed Apr 19 11:57:07 2017 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 19 Apr 2017 16:57:07 +0000 (UTC) Subject: [Histonet] Cytology pap staining In-Reply-To: References: Message-ID: <2006899598.4039819.1492621027153@mail.yahoo.com> Are the smears collected, fixed and treated as "usual" along ALL the steps?Ren? On Wednesday, April 19, 2017 12:54 PM, Charles Riley via Histonet wrote: I have been put in charge of figuring out why our pap stains are light on the hematoxylin. Everything was filtered and used fresh as per usual using the same protocol we have used for the past two years. If anyone has any suggestions as to how to fix this problem I would greatly appreciate it. If you need more information please let me know and I will get it for you to help assist you in assisting me -- Charles Riley HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed Apr 19 12:21:08 2017 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 19 Apr 2017 13:21:08 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin 4-19-2017 To Blog Or Not To Blog Message-ID: <113201d2b931$55d9d140$018d73c0$@earthlink.net> Hi Histonetters! How are you doing today? I hope today and the rest of your week is absolutely fantastic. I Have A Few Quick Questions For You I am thinking of restarting a blog I was writing a few years ago. I really hope that it will appeal to histology professionals at all levels. I don?t want to duplicate the efforts of a technical resource like the histonet. My blog will be more of a ?lifestyles? type blog with topics that appeal, inform and entertain you. The Questions I Have Are What are some topics that would interest you? What would you and your fellow histotechs enjoy reading about? Of course I have to pay the bills too so here is a list of my current positions. I am pretty excited about these opportunities because most of them are RELIA EXCLUSIVES! And all of them are ready to hire and eager to welcome you to their lab. All of the positions I represent are full time permanent positions with top employers nationwide. My clients offer excellent compensation, great benefits and relocation/sign on bonuses. HISTOLOGY LEADERSHIP- GA, WA, IL, WI, CA Histology Lab Manager ? Atlanta, GA- NEW POSITION DETAILS TO COME Histology Lab Manager ?SAN DIEGO, CA - GROWTH POSITION! Lead Histotech ? Longview, WA - DAYS!!! LEADERSHIP OPPTY! Histology Supervisor - North Chicago, IL ? RESEARCH OPPORTUNITY! Histology Supervisor ? Madison, WI ? GET OUT OF THE CLINICAL LAB! HT/HTL-VA, NC, TX, TN, GA Norfolk, VA ? 15K SIGN ON BONUS AND RELO! Wilmington, NC ? PLAY ON THE BEACH ALL DAY AND WORK NIGHTS! Atlanta, GA ? NEW POSITIONS DETAILS TO COME! Salem, VA ? DAY SHIFT AND WORK ALL AREAS OF THE HISTOLOGY LAB! Vancouver, WA-LEAD HISTOTECH POSITION! Longview, TX ? DERMPATH POSITION ? LEARN MOHS! Cranford, NJ ? DAY SHIFT, GREAT TEAM TO WORK WITH! If you or anyone you know might be interested in hearing more about any of these positions or would like help with a customized job search please contact me ASAP. I can be reached at relia1 at earthlink.net or toll free at 866-607-3542 or cell/text 407-353-5070. If you are even entertaining the idea of a job search in the next 12 months we need to talk NOW! *I would love to hear what you might find interesting as blog topics. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From jmcgough at clinlab.com Wed Apr 19 14:29:09 2017 From: jmcgough at clinlab.com (=?utf-8?Q?Jason_McGough?=) Date: Wed, 19 Apr 2017 13:29:09 -0600 Subject: [Histonet] Breast Her2Neu IHC vs FISH In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E95416D89@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E95416D89@HHCEXCHMB03.hhcsystem.org> Message-ID: Does anyone know or can you point me in the right direction to some literature about how to properly test for Her2Neu (IHC vs. FISH) on breast tumors if the cold ischemia time is greater than 1 hour or if the formalin fixation times are outside of the recommended time range? Also, what if the specimen has been placed in decal? We are struggling to find any documentation of how to properly test Her2Neu if the specimen is outside of these ranges. Thanks for your help! Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgough at clinlab.com www.clinlab.com From TNMayer at mdanderson.org Wed Apr 19 14:31:21 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Wed, 19 Apr 2017 19:31:21 +0000 Subject: [Histonet] processor died overnight Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8839EBB232@D1PWPEXMBX08.mdanderson.edu> To help this you could place the cassettes in the other processor in the last 100% for about 5 min, just to freshen them up. Then proceed with the remainder of the process as usual. FYI, this will be one of our discussion questions this year in our HTL program. T Toysha N. Mayer D.H.Sc., MBA, HT(ASCP) Instructor/Education Coordinator HTL Program MD Anderson School of Health Professions 713.563.3481 tnmayer at mdanderson.org Hello Histoworld, I came in this morning to find that the processor died halfway through process last night. The tissues are in 100% ETOH exactly half point. We do have a back- up processor. In your professional experiences, would these tissues be salvageable? Could I create a new program on the backup processor that finishes the process from that point and transfer the tissues over? Thanks. ------------------------------ Message: 5 Date: Wed, 19 Apr 2017 13:41:47 +0000 (UTC) From: Rene J Buesa To: Lauren Sweeney , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] processor died overnight Message-ID: <174147213.3806623.1492609307572 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 In 100% EthOL the tissues are completely "salvaged" and you can prepare the program to continue the steps until melted paraffin.If there are delicate tissue perhaps they will be "over-dried" but that is easily "compensated" during microtomy.Ren? On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet wrote: Hello Histoworld, I came in this morning to find that the processor died halfway through process last night. The tissues are in 100% ETOH exactly half point. We do have a back- up processor. In your professional experiences, would these tissues be salvageable? Could I create a new program on the backup processor that finishes the process from that point and transfer the tissues over? Thanks. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 19 Apr 2017 16:11:51 +0000 From: "Terri Braud" To: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] IF vs IHC another reason Message-ID: <48E053DDF6CE074DB6A7414BA05403F8130EAF at HRHEX03-HOS.holyredeemer.local> Content-Type: text/plain; charset="us-ascii" Another reason that IHC is used instead of IF is with IHC, one preserves the ability to see tissue/cell morphology through Light Microscopy at the same time as the visual IHC label. Morphology is difficult to see with IF, with the exception of the fluorescein labeled area. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 13 April 2017 11:10 PM Hello! I just need a help with a simple question...Is anyone can explain me what is the purpose between performing immunohistochemistry and Immunofluorescence? Thanks :) Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ------------------------------ Message: 7 Date: Wed, 19 Apr 2017 09:17:24 -0700 From: Caroline Miller To: Rene J Buesa Cc: Lauren Sweeney , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] processor died overnight Message-ID: <6156911D-EDE8-4C77-9C6D-36BA64BAF03F at 3scan.com> Content-Type: text/plain; charset=utf-8 Yes, totally +1 to Rene, they should be fine. (That has totally happened to me too)! Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 > On Apr 19, 2017, at 6:41 AM, Rene J Buesa via Histonet wrote: > > In 100% EthOL the tissues are completely "salvaged" and you can prepare the program to continue the steps until melted paraffin.If there are delicate tissue perhaps they will be "over-dried" but that is easily "compensated" during microtomy.Ren? > > On Wednesday, April 19, 2017 9:01 AM, Lauren Sweeney via Histonet wrote: > > > Hello Histoworld, > > I came in this morning to find that the processor died halfway through process last night. The tissues are in 100% ETOH exactly half point. We do have a back- up processor. In your professional experiences, would these tissues be salvageable? Could I create a new program on the backup processor that finishes the process from that point and transfer the tissues over? > > Thanks. Message: 8 Date: Wed, 19 Apr 2017 16:26:56 +0000 From: "McNabola, Angela" To: 'Caroline Miller' , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] processor died overnight Message-ID: <69AA9D996B56BF4EBCBB8EA666461B9326EBBB32 at mbx9vp.YNHH.ORG> Content-Type: text/plain; charset="utf-8" It has happened to us as well. I'm not sure where you work, but our processors have alarms that go to security. So I have gotten a call on off hours to come in and fix the problem or try to trouble shoot. Some of the newer processors can be linked to your phone, etc. -----Original Message----- From: Caroline Miller via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 19, 2017 12:17 PM To: Rene J Buesa Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] processor died overnight Yes, totally +1 to Rene, they should be fine. (That has totally happened to me too)! Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 ************************************* The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From Timothy.Morken at ucsf.edu Wed Apr 19 14:39:38 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 19 Apr 2017 19:39:38 +0000 Subject: [Histonet] Breast Her2Neu IHC vs FISH In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E95416D89@HHCEXCHMB03.hhcsystem.org> Message-ID: <761E2B5697F795489C8710BCC72141FF8E5CCA21@ex07.net.ucsf.edu> Jason, The only way is to validate for those conditions. You would need some specimens, including known controls, handled and processed in those same conditions and compare to samples processed under the acceptable conditions. You can do it, it will just take some time. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Jason McGough via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 19, 2017 12:29 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Breast Her2Neu IHC vs FISH Does anyone know or can you point me in the right direction to some literature about how to properly test for Her2Neu (IHC vs. FISH) on breast tumors if the cold ischemia time is greater than 1 hour or if the formalin fixation times are outside of the recommended time range? Also, what if the specimen has been placed in decal? We are struggling to find any documentation of how to properly test Her2Neu if the specimen is outside of these ranges. Thanks for your help! Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgough at clinlab.com www.clinlab.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcox90 at yahoo.com Wed Apr 19 15:28:00 2017 From: jcox90 at yahoo.com (Jill Cox) Date: Wed, 19 Apr 2017 20:28:00 +0000 (UTC) Subject: [Histonet] Scottsdale AZ Part-time Histology opening References: <208055925.4012325.1492633680503.ref@mail.yahoo.com> Message-ID: <208055925.4012325.1492633680503@mail.yahoo.com> Hello Histonetters, A North Scottsdale Dermatology practice is seeking a part-time Histology Technician. Two days a week, (approx 8-10 hours total) one day would be logging in and grossing biopsy and excision specimens, the next day would be cutting and staining. Currently no special stains are being performed. Must be able to work well on your own. Experience is a must in grossing Dermatology tissue specimens. Please reply to this email for more information. Thank you :) Jill Cox HT ASCP From marktarango at gmail.com Wed Apr 19 15:33:19 2017 From: marktarango at gmail.com (Mark Tarango) Date: Wed, 19 Apr 2017 13:33:19 -0700 Subject: [Histonet] Breast Her2Neu IHC vs FISH In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E95416D89@HHCEXCHMB03.hhcsystem.org> Message-ID: We add a comment when the specimen handling is outside of 2013 CAP/ASCO guidelines for breast or 2016 CAP/ASCO/ASCP guidelines for HER2 testing in GEA cancers. These guidelines and the data supplements have some good information. We usually have found that decal doesn't affect the IHC much if not excessive (but still add a comment) and we do attempt performing FISH and have to rely internal controls (normal 2-signal pattern on non-neoplastic cells) to know that the FISH reaction is appropriate. FISH working will depend on time in formalin before decal and time in decal. This changes from specimen to specimen, so it may be difficult to validate the full range of specimen variability. If you do FISH, the best trick is to re-apply the probe on the same slide that was already denatured and hybridized the previous day and run it through denaturation and hybridization again. This will bring out the signals in many cases. Since FISH has internal controls (normal 2-signal pattern in non-neoplastic cells), it can help give some confidence to a negative HER2 IHC result when specimen handling was compromised. I believe that is why the CAP question below says to perform "confirmatory analysis by in-situ hybridization". These are some of our comments Decal: This assay has not been validated on decalcified tissues. Results should be interpreted with caution given the possibility of false negativity on decalcified specimens. (we don't add this comment if the result is positive). Formalin fixation: The specimen did not meet optimal formalin fixation guidelines (fixed for xxx hours). This can play a role in loss of FISH signals. Repeat testing on an appropriately fixed specimen is recommended, if clinically feasible. Fixation and decal: The specimen did not meet optimal formalin fixation guidelines (fixed for xxx hours and xxx minutes). Both factors may play a role in weak or missing FISH signals. Repeat testing on a non-decalcified and properly fixed specimen is recommended, if clinically feasible. There is a CAP question that asks about some of those factors. Not sure if you are CAP-accredited or not. **REVISED** 07/28/2015 ANP.22983 HER2; ER/PgR - Fixation Phase I If the laboratory assesses HER2 protein over-expression by immunohistochemistry, HER2 (ERBB2) gene amplification by in situ hybridization, or estrogen/progesterone receptor expression by immunohistochemistry, there is a written procedure to ensure appropriate specimen fixation time. Anatomic Pathology Checklist 08.17.2016 NOTE: Specimens subject to these tests should be fixed in 10% neutral buffered formalin for at least six hours and up to 72 hours. The volume of formalin should be at least 10 times the volume of the specimen. Decalcification solutions with strong acids should not be used. For cases with negative HER2 results by IHC that were fixed outside these limits, consideration should be given to performing confirmatory analysis by in-situ hybridization. Laboratories must communicate the following fixation guidelines to clinical services: 1. Specimens should be immersed in fixative within one hour of the biopsy or resection 2. If delivery of a resection specimen to the pathology department is delayed (e.g. specimens from remote sites), the tumor should be bisected prior to immersion in fixative. In such cases, it is important that the surgeon ensure that the identity of the resection margins is retained in the bisected specimen; alternatively, the margins may be separately submitted. 3. The time of removal of the tissue and the time of immersion of the tissue in fixative should be recorded and submitted to the laboratory Communication may be through memoranda, website, phone, face-to-face meetings, or other means. The laboratory should consider monitoring compliance and contacting clients when these guidelines are not met. If specimens are fixed in a medium other than 10% neutral buffered formalin, the laboratory must perform a validation study showing that results are concordant with results from formalin-fixed tissues. Laboratories testing specimens obtained from another institution should have a policy that addresses time of fixation. Information on time of fixation may be obtained by appropriate questions on the laboratory?s requisition form. Reports should qualify any negative results for specimens not meeting the above guidelines. Reports containing ER, PgR or HER2 results for their predictive characteristics must specify the type of fixative used and the cold ischemia time. In addition, any treatment that may potentially alter immunoreactivity, such as decalcification, must be included. Mark Tarango On Wed, Apr 19, 2017 at 12:29 PM, Jason McGough via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Does anyone know or can you point me in the right direction to some > literature about how to properly test for Her2Neu (IHC vs. FISH) on breast > tumors if the cold ischemia time is greater than 1 hour or if the formalin > fixation times are outside of the recommended time range? Also, what if the > specimen has been placed in decal? We are struggling to find any > documentation of how to properly test Her2Neu if the specimen is outside of > these ranges. Thanks for your help! > > > > Jason McGough, HT(ASCP) > > Operations Manager > > Clinical Laboratory of the Black Hills > > 605-343-2267 > > jmcgough at clinlab.com > > www.clinlab.com > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tony.henwood at health.nsw.gov.au Wed Apr 19 18:42:00 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 19 Apr 2017 23:42:00 +0000 Subject: [Histonet] processor died overnight In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF95540D64@SVDCMBX-MEX008.nswhealth.net> Yep start at last alcohol in other processor, xylene & wax as usual. If the tissues have remained covered in alcohol, you should not have a problem Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Lauren Sweeney via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 19 April 2017 10:44 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] processor died overnight Hello Histoworld, I came in this morning to find that the processor died halfway through process last night. The tissues are in 100% ETOH exactly half point. We do have a back- up processor. In your professional experiences, would these tissues be salvageable? Could I create a new program on the backup processor that finishes the process from that point and transfer the tissues over? Thanks. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From tony.henwood at health.nsw.gov.au Wed Apr 19 18:46:49 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 19 Apr 2017 23:46:49 +0000 Subject: [Histonet] Cytology pap staining In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF95540D92@SVDCMBX-MEX008.nswhealth.net> I would also check the fixation. Do the smears look air-dried. The larger nuclei, following air-drying do not concentrate the Hx as much as prompt alcohol fixation resulting in paler stained nuclei. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 20 April 2017 2:31 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cytology pap staining I have been put in charge of figuring out why our pap stains are light on the hematoxylin. Everything was filtered and used fresh as per usual using the same protocol we have used for the past two years. If anyone has any suggestions as to how to fix this problem I would greatly appreciate it. If you need more information please let me know and I will get it for you to help assist you in assisting me -- Charles Riley HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From garethdavisyuma at gmail.com Thu Apr 20 09:19:49 2017 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Thu, 20 Apr 2017 07:19:49 -0700 Subject: [Histonet] Now I have ribbons Message-ID: Thanks to everyone for their response to my problem with cutting last week. Things are much better, getting ribbons, now that I have increased my angle to 8. The humidity in Yuma is now actually above 10%, so I really think that helps too. I did try trimming the blocks to sharper corners, but that didn't help much. For some reason when trimming it made the block "sticky" , even when I tried to smooth it out, and the ribbon would stick onto the block. But, like I said I am getting ribbons with the angle. Thanks again. -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From AJohnson at aipathology.com Thu Apr 20 10:14:34 2017 From: AJohnson at aipathology.com (Amy Johnson) Date: Thu, 20 Apr 2017 15:14:34 +0000 Subject: [Histonet] test Message-ID: test Amylin Johnson, B.S. HTL(ASCP) Associates in Pathology Wausau Wi 54401 715-847-2130 From patpxs at gmail.com Thu Apr 20 10:24:57 2017 From: patpxs at gmail.com (P Sicurello) Date: Thu, 20 Apr 2017 08:24:57 -0700 Subject: [Histonet] Microwave for EM? Message-ID: Good Morning Listers, Happy Friday Eve! Who out there is microwave processing tissue for EM? Which microwave processor are you using? Are there any limitations (can?t process certain tissues, tissues look significantly different than traditional processing, makes a mess-that type of thing)? We are considering switching to microwave processing. Currently we are using a Lynx. Send me your opinions, experiences, and thoughts about microwave processing for EM. Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From AJohnson at aipathology.com Thu Apr 20 11:29:25 2017 From: AJohnson at aipathology.com (Amy Johnson) Date: Thu, 20 Apr 2017 16:29:25 +0000 Subject: [Histonet] alcohol labels Message-ID: We have started using Formalin labels that have the GHS symbols on them and would like to find alcohol labels with GHS symbols on them as well.....what do all you in HistoLand use??? Amylin Johnson, B.S. HTL(ASCP) Associates in Pathology Wausau Wi 54401 715-847-2130 From mills at 3scan.com Thu Apr 20 14:13:35 2017 From: mills at 3scan.com (Caroline Miller) Date: Thu, 20 Apr 2017 12:13:35 -0700 Subject: [Histonet] CD8 for rat - also happy to take human suggestions Message-ID: Hi Histonet! I am on the search for a good CD8 antibody, I have done a literature dive and cannot find a consistently used one. I currently have abcam ab131500 Rb (monoclonal) and it is just not working at all. Does anyone have any advice in this space. I am totally interested in clinical human antibodies too as they often have cross reactivity. Any other nuances in the staining that people have found would also be great. thanks folks! mills -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From mills at 3scan.com Fri Apr 21 09:18:20 2017 From: mills at 3scan.com (Caroline Miller) Date: Fri, 21 Apr 2017 07:18:20 -0700 Subject: [Histonet] CD8 for rat - also happy to take human suggestions In-Reply-To: References: Message-ID: Thank you for all the suggestions Histonet, you wonders of knowledge! Happy to share if anyone wants a collated list, let me know Happy Friday! mills On Thu, Apr 20, 2017 at 12:13 PM, Caroline Miller wrote: > Hi Histonet! > > I am on the search for a good CD8 antibody, I have done a literature dive > and cannot find a consistently used one. I currently have abcam ab131500 Rb > (monoclonal) and it is just not working at all. > > Does anyone have any advice in this space. I am totally interested in > clinical human antibodies too as they often have cross reactivity. > > Any other nuances in the staining that people have found would also be > great. > > thanks folks! > > mills > > -- > Caroline Miller (mills) > Director of Histology > 3Scan.com > 415 2187297 <(415)%20218-7297> > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From b-frederick at northwestern.edu Fri Apr 21 10:10:28 2017 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Fri, 21 Apr 2017 15:10:28 +0000 Subject: [Histonet] sectioning issues? In-Reply-To: <1492787064903.31617@northwestern.edu> References: <1492722529413.35959@northwestern.edu>, <1492781211497.7265@northwestern.edu>, <2d3db09557fa4d73b58da9b799c975c2@evcspmbx03.ads.northwestern.edu>, <1492783083223.92018@northwestern.edu> <1492787064903.31617@northwestern.edu> Message-ID: <5474a407e20a4ba5817409a56f86818b@evcspmbx03.ads.northwestern.edu> You may have to call a tech at thermos to ask...... It still sounds like it it's the microtome as we know they cut. Join Histonet or the NSH facebook page and you can ask there as well. I may just be missing something obvious. histonet at lists.utsouthwestern.edu for histonet. It is a forum. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu From: Elizabeth Jane Lux Sent: Friday, April 21, 2017 10:04 AM To: Bernice Frederick Subject: Re: sectioning issues? Thanks so much for your help! Frustratingly having issues when I move into sectioning. Ribbons look square at trim mode (20u) but when I try to get my sections, I'm getting inconsistent slicing, almost like it's compressing it at times. Or I guess not advancing the specimen correctly? Perhaps the arm needs maintenance? Oh, and I was completely wrong on the model, it's a Thermo Finesse ME+ Liz ________________________________ From: Elizabeth Jane Lux Sent: Friday, April 21, 2017 8:58 AM To: Bernice Frederick Subject: Re: sectioning issues? Awesome! I'll bring the block(s) over now, thanks! Elizabeth Lux Research Tech 3 Feinberg Cardiovascular Research Institute Northwestern University Lurie Building, 10-220 312.503.6368 lab phone 312.503.0219 fax ________________________________ From: Bernice Frederick Sent: Friday, April 21, 2017 8:55 AM To: Elizabeth Jane Lux Subject: RE: sectioning issues? You can always pop by and let me see..... Now would be fine. I am gone all next week. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu From: Elizabeth Jane Lux Sent: Friday, April 21, 2017 8:27 AM To: Bernice Frederick Subject: Re: sectioning issues? Tissue is kidney and heart. Thickness was going off of past work done in the lab, utilizing the guidelines in the AFIP Lab methods in Histo red book. The machine had some maintenance done last year, though the tech seemed really green and unsure of himself. The block seems secure to the cassette. Thanks for your thoughts! Elizabeth Lux Research Tech 3 Feinberg Cardiovascular Research Institute Northwestern University Lurie Building, 10-220 312.503.6368 lab phone 312.503.0219 fax ________________________________ From: Bernice Frederick Sent: Friday, April 21, 2017 7:43 AM To: Elizabeth Jane Lux Subject: RE: sectioning issues? Hi Liz, What kind of tissue are you sectioning? Bear in mind,the microtome you are using may have never had any maintenance on it. And why are you cutting at 6? Unless you are doing neuro or looking for amyloid, 4 um is the best thickness. The block itself may be coming away from the cassette. This can cause said issues. Wiggle the paraffin and see if it is not coming away from the cassette. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu From: Elizabeth Jane Lux Sent: Thursday, April 20, 2017 4:09 PM To: Bernice Frederick Subject: sectioning issues? Hey Bernice, Wonder if I could ask your opinion on some microtome troubleshooting. I'm having a few tricky paraffin blocks. Facing it gets me nice smooth ribbons, but when I move to the sections (6micron) I get inconsistent results. It will give me a full section, then a half, then full, then half, and so on . . Moved to a test block to work out the kinks, and adjusted the angle slightly, tightened up the blade holder, and things seemed to be ribboning better. But then still having issues with the sample block of interest. Anyway, thought it was worth asking if you had any advice. Thanks! Liz Elizabeth Lux Research Tech 3 Feinberg Cardiovascular Research Institute Northwestern University Lurie Building, 10-220 312.503.6368 lab phone 312.503.0219 fax From Blanca.Lopez at UTSouthwestern.edu Fri Apr 21 11:08:37 2017 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Fri, 21 Apr 2017 16:08:37 +0000 Subject: [Histonet] looking for a slider printer Message-ID: <6a54a9d61a4b4c5fa005e53a422af9ce@SWMS13MAIL12.swmed.org> Dear Histonets!!! We are looking to buy a slide printer for a low cost, durable and easy to work. W are a very small lab, we don't' need anything fancy. If you know somebody or have a good experience with one please let me know. Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From wbenton at cua.md Fri Apr 21 11:16:43 2017 From: wbenton at cua.md (Walter Benton) Date: Fri, 21 Apr 2017 16:16:43 +0000 Subject: [Histonet] looking for a slider printer In-Reply-To: <6a54a9d61a4b4c5fa005e53a422af9ce@SWMS13MAIL12.swmed.org> References: <6a54a9d61a4b4c5fa005e53a422af9ce@SWMS13MAIL12.swmed.org> Message-ID: <1a28a2b9b8484d75a5f488f8386f7380@MAIL01.GCU-MD.local> http://accuplace.com/products/pslim/ Manufacturers of the original slidemate (Thermo Fisher). Solid instruments with minimal fuss. Keep them clean and maintained according to manufacturer specs and they work well. Using proper Thermal Slides are also important for getting a good print and not destroying the print head. Feel free to reach out if you have additional questions. Walter Benton HT(ASCP)QIHC -----Original Message----- From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, April 21, 2017 12:09 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] looking for a slider printer Dear Histonets!!! We are looking to buy a slide printer for a low cost, durable and easy to work. W are a very small lab, we don't' need anything fancy. If you know somebody or have a good experience with one please let me know. Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From lblazek at digestivespecialists.com Fri Apr 21 11:27:24 2017 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Fri, 21 Apr 2017 12:27:24 -0400 Subject: [Histonet] looking for a slider printer In-Reply-To: <6a54a9d61a4b4c5fa005e53a422af9ce@SWMS13MAIL12.swmed.org> References: <6a54a9d61a4b4c5fa005e53a422af9ce@SWMS13MAIL12.swmed.org> Message-ID: <5A2BD13465E061429D6455C8D6B40E391A45311EFA@IBMB7Exchange.digestivespecialists.com> Blanca, We have had the Primera Slide printer for several years now. It's a great small footprint printer and easily connects to our LIS. Check with Creative Waste Solutions. http://cwsincorp.com/ They sell them. Linda Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, April 21, 2017 12:09 PM To: Subject: [Histonet] looking for a slider printer Dear Histonets!!! We are looking to buy a slide printer for a low cost, durable and easy to work. W are a very small lab, we don't' need anything fancy. If you know somebody or have a good experience with one please let me know. Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann at aol.com Fri Apr 21 13:32:34 2017 From: thisisann at aol.com (Ann Specian) Date: Fri, 21 Apr 2017 14:32:34 -0400 Subject: [Histonet] Sakura Automated Embedding Center and Express processor Message-ID: <15b91c98458-612d-343c4@webprd-m01.mail.aol.com> Is anyone familiar with the Sakura Automated Embeddingcenter or their Express processor? If so, can you let me know yourthoughts. Thanks, Ann From carl.hobbs at kcl.ac.uk Fri Apr 21 14:03:02 2017 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 21 Apr 2017 19:03:02 +0000 Subject: [Histonet] CD8 for rat - also happy to take human Message-ID: I am very interested to have your information! Thank you Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From smt226 at hotmail.com Sun Apr 23 03:51:19 2017 From: smt226 at hotmail.com (Shauna Talboys) Date: Sun, 23 Apr 2017 08:51:19 +0000 Subject: [Histonet] temp job Message-ID: Hello, When were you looking to hire a nistotech for the temp job? ASAP? Thank you, Shauna Talboys From LRaff at uropartners.com Mon Apr 24 08:09:14 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 24 Apr 2017 13:09:14 +0000 Subject: [Histonet] Lab related blog---and Happy Lab Week Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF1136E0D8@COLOEXCH01.uropartners.local> Congrats to all the great histotechs and staffs during National Lab Week. I couldn't do my job without the wonderful people creating crisp clean slides and sparkling special stains. And they make the lab a fun place to come to every day. Today's blog is lab related, though not directly histology related. I still feel it is important to share: http://www.chicagonow.com/downsize-maybe/2017/04/lets-talk-about-psa/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From NRich at bmhsc.org Mon Apr 24 10:13:42 2017 From: NRich at bmhsc.org (Nina J. Rich) Date: Mon, 24 Apr 2017 15:13:42 +0000 Subject: [Histonet] CAP code for H&E Message-ID: Does anyone know if CAP requires that labs run an H&E control daily? If so what is the # for that requirement? Also, for pap stains? From wbenton at cua.md Mon Apr 24 11:09:32 2017 From: wbenton at cua.md (Walter Benton) Date: Mon, 24 Apr 2017 16:09:32 +0000 Subject: [Histonet] CAP code for H&E In-Reply-To: References: Message-ID: Hope this helps. ANP.10042 Histologic Prep Quality Phase I There is a written procedure that describes the process by which pathologists or their designees provide feedback to the histology laboratory on the quality of histologic preparations. This procedure must include the daily recording of the quality of the histologic preparations for each day of tissue processing and slide preparation. NOTE: Histologic preparations refer to H & E sections, histochemical stains, immunohistochemistry preparations, and in situ hybridization preparations. This requirement applies to laboratories that process and interpret histologic preparations at the same location, as well as laboratories that interpret histologic preparations processed at another laboratory (regardless of that outside laboratory's accrediting organization). Records of such feedback and corrective action taken when problems are identified may be incorporated into the laboratory's quality management program. Specific quality control requirements for special stains, immunohistochemistry, and other special studies are found elsewhere in this checklist. Evidence of Compliance: ? Records of feedback and corrective action for problems identified with histologic prep quality ANP.11734 Slide Quality Phase II Slides are of sufficient quality for diagnosis. NOTE: Histopathology slides must be of adequate technical quality to be diagnostically useful. Criteria to evaluate include adequate tissue fixation, processing, thickness of sections, absence of interfering tissue folds and tears, and good staining technique and cover slipping. For hematoxylin and eosin and other routine stains, the patient slide serves as the internal control to ensure adequate staining technique. The sections must be cut from sufficient depth in the block to include the entire tissue plane. **REVISED** 07/28/2015 CYP.04300 Daily QC Phase II There are records of daily review of the technical quality of cytologic preparations by the pathologist or supervisory-level cytotechnologist. NOTE: The technical quality of cytologic preparations must be checked daily (on days processing occurs). This includes checking all stains for predicted staining characteristics each day of use. This check must include all of the types of preparations seen that day such as cytospins, cell blocks, and liquid based preparations. If preparation and staining is performed by a different laboratory, there must be a procedure for the laboratory performing the preparation and staining to verify the acceptability of the quality of preparations and the acceptability of controls (if needed) before transfer. Records of this verification must be readily available to the laboratory performing interpretations. There should also be a mechanism for feedback from the interpreting laboratory to the laboratory that prepared the slides of any issues with the preparations. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: Nina J. Rich via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, April 24, 2017 11:14 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CAP code for H&E Does anyone know if CAP requires that labs run an H&E control daily? If so what is the # for that requirement? Also, for pap stains? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From bcooper at chla.usc.edu Mon Apr 24 13:54:26 2017 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Mon, 24 Apr 2017 18:54:26 +0000 Subject: [Histonet] Empty Chemical Containers Message-ID: Happy Lab Week Histonet! How are you all disposing of empty chemical containers? Triple rinsing and throwing them in regular trash (with the labels defaced,) or having the empty containers disposed of by your outside chemical contractors? I'm sure this answer will vary from state to state, and institution to institution (and chemical to chemical for that matter). Sorry if this has been asked previously-I searched the archives, and an answer wasn't readily found. This came up during safety rounds the other day, and our institution's policy on this is pretty vague. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From Kelly.Pairan at nationwidechildrens.org Tue Apr 25 07:37:46 2017 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Tue, 25 Apr 2017 12:37:46 +0000 Subject: [Histonet] HP antibody Message-ID: Hello Histoworld, Happy Lab Week! We have not been able to get H. pylori antibody from our current supplier for a while. Who is the supplier for your lab and do you have any stock issue? Thanks and have a great day! Kelly Pairan, HT (ASCP) QIHC(ASCP) From Richard.Cartun at hhchealth.org Tue Apr 25 09:02:25 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 25 Apr 2017 14:02:25 +0000 Subject: [Histonet] ICD-10 Codes Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E9541A7DC@HHCEXCHMB03.hhcsystem.org> What do you do when you receive a pathology consult and it is missing an ICD-10 code? I am being told that we cannot accession it until we get a code from the submitting doctor's office, and that we cannot add one. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From INAIR at coh.org Tue Apr 25 09:13:07 2017 From: INAIR at coh.org (Nair, Indu) Date: Tue, 25 Apr 2017 14:13:07 +0000 Subject: [Histonet] Help with eosin stain Message-ID: Good morning Histoneters! I am having a problem with eosin staining getting washed away in the upgrades. Eosin was prepared in isopropyl alcohol, followed one of the protocols online. Please advice. Thank you! indu --------------------------------------------------------------------- -SECURITY/CONFIDENTIALITY WARNING- This message (and any attachments) are intended solely for the individual or entity to which they are addressed. This communication may contain information that is privileged, confidential, or exempt from disclosure under applicable law (e.g., personal health information, research data, financial information). Because this e-mail has been sent without encryption, individuals other than the intended recipient may be able to view the information, forward it to others or tamper with the information without the knowledge or consent of the sender. If you are not the intended recipient, or the employee or person responsible for delivering the message to the intended recipient, any dissemination, distribution or copying of the communication is strictly prohibited. If you received the communication in error, please notify the sender immediately by replying to this message and deleting the message and any accompanying files from your system. If, due to the security risks, you do not wish to receive further communications via e-mail, please reply to this message and inform the sender that you do not wish to receive further e-mail from the sender. (LCP301) --------------------------------------------------------------------- From Megan.Dishop at childrensmn.org Tue Apr 25 09:27:52 2017 From: Megan.Dishop at childrensmn.org (Megan Dishop) Date: Tue, 25 Apr 2017 09:27:52 -0500 Subject: [Histonet] [EXTERNAL] ICD-10 Codes In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E9541A7DC@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E9541A7DC@HHCEXCHMB03.hhcsystem.org> Message-ID: <58FF1698.D202.00E3.1@childrensmn.org> I don't think that is necessary. Pathology services are coded by the pathologic diagnosis, unlike laboratory testing that requires an ICD10 code or reason for test request upfront. The blog entry below mentions this distinction.... http://www.mckesson.com/bps/blog/preparing-for-icd-10-by-addressing-incomplete-diagnosis-information-received-errors/ Megan K. Dishop MD Medical Director, Pediatric Anatomic Pathology Children's Hospitals and Clinics of Minnesota Laboratories 2525 Chicago Ave S. MS32-B600, Minneapolis, MN 55404 USA Phone: 612-813-6521 Fax: 612-813-7721 Email: megan.dishop at childrensMN.org Adjoint Professor of Pediatrics, University of Colorado School of Medicine >>> "Cartun, Richard via Histonet" 4/25/2017 9:02 AM >>> What do you do when you receive a pathology consult and it is missing an ICD-10 code? I am being told that we cannot accession it until we get a code from the submitting doctor's office, and that we cannot add one. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. From LRaff at uropartners.com Tue Apr 25 10:44:49 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 25 Apr 2017 15:44:49 +0000 Subject: [Histonet] [EXTERNAL] ICD-10 Codes In-Reply-To: <58FF1698.D202.00E3.1@childrensmn.org> References: <9215BD4B0BA1B44D962A71C758B68D2E9541A7DC@HHCEXCHMB03.hhcsystem.org> <58FF1698.D202.00E3.1@childrensmn.org> Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF11379122@COLOEXCH01.uropartners.local> Interesting--we always require and ICD from our submitting providers even though we are all part of the same group. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: Megan Dishop via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, April 25, 2017 9:28 AM To: Richard Cartun; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] [EXTERNAL] ICD-10 Codes I don't think that is necessary. Pathology services are coded by the pathologic diagnosis, unlike laboratory testing that requires an ICD10 code or reason for test request upfront. The blog entry below mentions this distinction.... http://www.mckesson.com/bps/blog/preparing-for-icd-10-by-addressing-incomplete-diagnosis-information-received-errors/ Megan K. Dishop MD Medical Director, Pediatric Anatomic Pathology Children's Hospitals and Clinics of Minnesota Laboratories 2525 Chicago Ave S. MS32-B600, Minneapolis, MN 55404 USA Phone: 612-813-6521 Fax: 612-813-7721 Email: megan.dishop at childrensMN.org Adjoint Professor of Pediatrics, University of Colorado School of Medicine >>> "Cartun, Richard via Histonet" >>> 4/25/2017 9:02 AM >>> What do you do when you receive a pathology consult and it is missing an ICD-10 code? I am being told that we cannot accession it until we get a code from the submitting doctor's office, and that we cannot add one. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SteveM at mcclainlab.com Tue Apr 25 14:16:55 2017 From: SteveM at mcclainlab.com (Steve McClain) Date: Tue, 25 Apr 2017 19:16:55 +0000 Subject: [Histonet] Histonet Digest, Vol 161, Issue 24 ICD10 In-Reply-To: References: Message-ID: 1 Google it or look it up Or 2 use an allpurpose icd10 as a placeholder, e.g. For skin one can use r23.8 other skin changes, unless you can find an icd10 for "no icd10". 3 feeling bold- use V91.07XA (burn due to water skis) or V96.00XS, which outlines an unspecified balloon accident injuring occupant. Steve A. McClain, MD > On Apr 25, 2017, at 13:21, "histonet-request at lists.utsouthwestern.edu" wrote: > > What do you do when you receive a pathology consult and it is missing an ICD-10 code? I am being told that we cannot accession it until we get a code from the submitting doctor's office, and that we cannot add one. Thank you. > > Richard > > Richard W. Cartun, MS, PhD From JMaslanka at stpetes.org Tue Apr 25 16:37:56 2017 From: JMaslanka at stpetes.org (Joseph Maslanka) Date: Tue, 25 Apr 2017 15:37:56 -0600 Subject: [Histonet] VIAS no more Message-ID: Our VIAS finally crashed, I would like your input for a replacement image analysis systems for ER PR, HER2neu and Ki67s. Thanks Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. Communication of electronic protected health information (ePHI) is protected under the Health Insurance Portability and Accountability Act (HIPAA) Act of 1996. Electronic mail (e-mail) communication is not encrypted or secure. The HIPAA Security Rule allows for patients to initiate communication of personal health information over this medium and for providers to respond accordingly with the understanding that privacy of communication is not guaranteed. From karenlahti3 at gmail.com Tue Apr 25 22:00:43 2017 From: karenlahti3 at gmail.com (Karen Lahti) Date: Tue, 25 Apr 2017 20:00:43 -0700 Subject: [Histonet] HP antibody In-Reply-To: References: Message-ID: <462353D0-4E01-47F6-92B7-4B897579E407@gmail.com> We have used Biocare?s Hp concentrate and predilute for years. Very consistent and the concentrate is strong. There is no background and it is very clean. Karen Lahti, HT, QIHC Lab Manager Arizona Digestive Health Phoenix, AZ > On Apr 25, 2017, at 5:37 AM, Pairan, Kelly via Histonet wrote: > > Hello Histoworld, > Happy Lab Week! We have not been able to get H. pylori antibody from our current supplier for a while. Who is the supplier for your lab and do you have any stock issue? > > Thanks and have a great day! > Kelly Pairan, HT (ASCP) QIHC(ASCP) > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NRich at bmhsc.org Wed Apr 26 07:56:46 2017 From: NRich at bmhsc.org (Nina J. Rich) Date: Wed, 26 Apr 2017 12:56:46 +0000 Subject: [Histonet] CAP code for H&E In-Reply-To: References: , Message-ID: <1032fa43299743deb4043e0b240cd176@bmhsc.org> Thanks for information it was very helpful. Happy Lab Week to all!! ________________________________________ From: Walter Benton Sent: Monday, April 24, 2017 12:09 PM To: Nina J. Rich Cc: histonet at lists.utsouthwestern.edu Subject: RE: CAP code for H&E Hope this helps. ANP.10042 Histologic Prep Quality Phase I There is a written procedure that describes the process by which pathologists or their designees provide feedback to the histology laboratory on the quality of histologic preparations. This procedure must include the daily recording of the quality of the histologic preparations for each day of tissue processing and slide preparation. NOTE: Histologic preparations refer to H & E sections, histochemical stains, immunohistochemistry preparations, and in situ hybridization preparations. This requirement applies to laboratories that process and interpret histologic preparations at the same location, as well as laboratories that interpret histologic preparations processed at another laboratory (regardless of that outside laboratory's accrediting organization). Records of such feedback and corrective action taken when problems are identified may be incorporated into the laboratory's quality management program. Specific quality control requirements for special stains, immunohistochemistry, and other special studies are found elsewhere in this checklist. Evidence of Compliance: ? Records of feedback and corrective action for problems identified with histologic prep quality ANP.11734 Slide Quality Phase II Slides are of sufficient quality for diagnosis. NOTE: Histopathology slides must be of adequate technical quality to be diagnostically useful. Criteria to evaluate include adequate tissue fixation, processing, thickness of sections, absence of interfering tissue folds and tears, and good staining technique and cover slipping. For hematoxylin and eosin and other routine stains, the patient slide serves as the internal control to ensure adequate staining technique. The sections must be cut from sufficient depth in the block to include the entire tissue plane. **REVISED** 07/28/2015 CYP.04300 Daily QC Phase II There are records of daily review of the technical quality of cytologic preparations by the pathologist or supervisory-level cytotechnologist. NOTE: The technical quality of cytologic preparations must be checked daily (on days processing occurs). This includes checking all stains for predicted staining characteristics each day of use. This check must include all of the types of preparations seen that day such as cytospins, cell blocks, and liquid based preparations. If preparation and staining is performed by a different laboratory, there must be a procedure for the laboratory performing the preparation and staining to verify the acceptability of the quality of preparations and the acceptability of controls (if needed) before transfer. Records of this verification must be readily available to the laboratory performing interpretations. There should also be a mechanism for feedback from the interpreting laboratory to the laboratory that prepared the slides of any issues with the preparations. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: Nina J. Rich via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, April 24, 2017 11:14 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CAP code for H&E Does anyone know if CAP requires that labs run an H&E control daily? If so what is the # for that requirement? Also, for pap stains? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From NRich at bmhsc.org Wed Apr 26 08:15:13 2017 From: NRich at bmhsc.org (Nina J. Rich) Date: Wed, 26 Apr 2017 13:15:13 +0000 Subject: [Histonet] primera cassette printer and interface LIS systems Message-ID: <55e7d7651a2f4a15a1e48d2b93a09dd5@bmhsc.org> Good morning ya'll, Happy lab week! Just wondering if anyone out there has the Primera cassette and slide printers that use Meditech? If so, are they interfaced? We are trying to get ours to work through using a text file without actually interfacing with Meditech. Has anyone tried this? Please let me know if you have gotten yours to work. You can also call me at 843-522-5159. Thanks. Jean From melissa at alliedsearchpartners.com Wed Apr 26 09:56:45 2017 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Wed, 26 Apr 2017 14:56:45 +0000 Subject: [Histonet] Perm Histotech Job for Early AM Shift-Lakeland, FL Message-ID: Hello and Happy Lab Week!! I have a permanent/full time early morning Histotech position in Lakeland, FL. Start time of about 1:30am and end time around 9am. Contact me for more details if you are interested. Thank you! Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 From criley at dpspa.com Thu Apr 27 06:51:18 2017 From: criley at dpspa.com (Charles Riley) Date: Thu, 27 Apr 2017 07:51:18 -0400 Subject: [Histonet] P16 Message-ID: Does anyone order P16 from Biogenix? I am trying to purchase this antibody from them but can not get ahold of the company to do so. If you do can you please send me the link or phone number to contact them. Thanks -- Charles Riley HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From DavidKemlerLLC at msn.com Thu Apr 27 07:51:15 2017 From: DavidKemlerLLC at msn.com (David) Date: Thu, 27 Apr 2017 12:51:15 +0000 Subject: [Histonet] CA Procedure for Waste Storage and Disposal Message-ID: I have been racking my brain trying to find a histology procedure that addresses CA regulations for disposal of formaldehyde, xylene and alcohol. If any CA histo-person can send it off to me, I would greatly appreciate your help. BTW, I know of a histotech position for a lab in Marin County, CA. Just saying. Dave From criley at dpspa.com Thu Apr 27 07:57:21 2017 From: criley at dpspa.com (Charles Riley) Date: Thu, 27 Apr 2017 08:57:21 -0400 Subject: [Histonet] Peloris II Tissue Processor Message-ID: Does anyone use the Peloris II processor from Leica. We are looking to purchase and I want some background information 1. What are its advantages? 2. What are the disadvantages? 3. Have you experience any technical issue/downtime and how quickly did Leica respond to fix the problem? 4. On a scale of 1 to 5 with 5 being the best how would you compare it to other processors you have used before? Any help would greatly be appreciated -- Charles Riley HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From TNMayer at mdanderson.org Thu Apr 27 15:34:23 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Thu, 27 Apr 2017 20:34:23 +0000 Subject: [Histonet] . Peloris II Tissue Processor Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8839EC9996@D1PWPEXMBX08.mdanderson.edu> Charles, The Peloris is great! The advantages is that it is very user friendly. You can run two different protocols at the same time, due to the dual retorts. It conserves reagents, because it calculates based on the number of cassettes you tell it you ran. It automatically rotates the reagents. We also had a battery backup in case of emergencies. Disadvantages are if you have a low to moderate workload, it may be too costly. It is very large in my opinion, so you need more floor space than other processors. The maintenance plan is well worth its large price. The lab I worked at during Hurricane Ike in Houston did not have their instrument on a 'red plug', so our processor stopped in the middle of the run. Leica got to us within a week. Any other time they were able to help us within a day or so. On a scale of 1 to 5, I give the Peloris a 5. ------------------------------ Message: 3 Date: Thu, 27 Apr 2017 08:57:21 -0400 From: Charles Riley To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Peloris II Tissue Processor Message-ID: Content-Type: text/plain; charset=UTF-8 Does anyone use the Peloris II processor from Leica. We are looking to purchase and I want some background information 1. What are its advantages? 2. What are the disadvantages? 3. Have you experience any technical issue/downtime and how quickly did Leica respond to fix the problem? 4. On a scale of 1 to 5 with 5 being the best how would you compare it to other processors you have used before? Any help would greatly be appreciated -- Charles Riley HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 161, Issue 26 ***************************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From isabelsoto1162 at yahoo.com Thu Apr 27 16:01:57 2017 From: isabelsoto1162 at yahoo.com (Isabel Soto) Date: Thu, 27 Apr 2017 21:01:57 +0000 (UTC) Subject: [Histonet] Peloris II Tissue Processor In-Reply-To: References: Message-ID: <1504961958.2555835.1493326917477@mail.yahoo.com> Don't get the peloris. Get the tissue tek VIP Sent from Yahoo Mail on Android On Thu, Apr 27, 2017 at 6:01 AM, Charles Riley via Histonet wrote: Does anyone use the Peloris II processor from Leica. We are looking to purchase and I want some background information 1. What are its advantages? 2. What are the disadvantages? 3. Have you experience any technical issue/downtime and how quickly did Leica respond to fix the problem? 4. On a scale of 1 to 5 with 5 being the best how would you compare it to other processors you have used before? Any help would greatly be appreciated -- Charles Riley HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford at DignityHealth.org Fri Apr 28 06:50:48 2017 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Fri, 28 Apr 2017 04:50:48 -0700 Subject: [Histonet] Prisma stainer Message-ID: Good Morning, I am not familiar with Sakura Prisma Autostainer. I heard the stainer can come with a tape coverslipper. Is the stainer, link and coverslipper all one unit or is it individual. I maybe getting a stainer that has the link does that mean that is the coverslipper or just the link between the stainer and coverslipper? How wide is the stainer with the coverslipper. I looked up the information but wanted some real user input. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you From rp7844 at gmail.com Fri Apr 28 10:43:26 2017 From: rp7844 at gmail.com (R P) Date: Fri, 28 Apr 2017 08:43:26 -0700 Subject: [Histonet] Nuclei isolation from ethanol-fixed tissue Message-ID: <000101d2c036$2dc66770$89533650$@gmail.com> Dear Histonet Users, What methods can be used for quantitative isolation of intact nuclei from brain tissue cryosections fixed in ethanol? The purpose is to extract RNA from single nuclei. The greater the yield of nuclei from small sections, and the lower the bias against a particular cell type, the better. Thank you, RB Swedish Medical Center From mneglia at trajanscimed.com Fri Apr 28 11:55:37 2017 From: mneglia at trajanscimed.com (Marc Neglia) Date: Sat, 29 Apr 2017 02:55:37 +1000 Subject: [Histonet] Pathology slide survey - AMAZON GIFT CARD OFFER In-Reply-To: <61E7E1B920537D4BABCCDF8D8B6E70D1062B2A36E464@mifune1> References: <61E7E1B920537D4BABCCDF8D8B6E70D1062B2A2FD9E3@mifune1> <61E7E1B920537D4BABCCDF8D8B6E70D1062B2A36E464@mifune1> Message-ID: <61E7E1B920537D4BABCCDF8D8B6E70D1062B2A36EEB4@mifune1> I hope you all had an enjoyable LAB WEEK!! THIS WILL BE A FINAL REMINDER: Winners to be chosen in early May. Thanks to all for your responses. -----Original Message----- From: Marc Neglia via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 5, 2017 4:12 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pathology slide survey - AMAZON GIFT CARD OFFER Hello Histonetters, I am conducting a study to learn about your experiences in the laboratory with microscope slides. The information gathered will help assist with product development decisions that are aimed at improving patient outcomes and optimizing your pathology workflow. To participate in this short, 5 minute survey, please click on the link below: https://www.surveymonkey.com/r/BKNBBWV As a token of appreciation, we will randomly select four (4) respondents to receive a $50 Amazon gift card after the survey has been completed. I would like to thank you in advance for your participation, and I look forward to viewing your responses. Best regards, Marc Marc Neglia Trajan Scientific and Medical mneglia at trajanscimed.com www.trajanscimed.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Blanca.Lopez at UTSouthwestern.edu Fri Apr 28 15:52:20 2017 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Fri, 28 Apr 2017 20:52:20 +0000 Subject: [Histonet] cassette and slide printer Message-ID: <9a5c802458014db7891d394af67b19e2@SWMS13MAIL12.swmed.org> Dear Histonettes, I am on my search of looking for a cassette and slide printer for a small lab. Any recommendations are welcome:) Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From isabelsoto1162 at yahoo.com Fri Apr 28 17:35:38 2017 From: isabelsoto1162 at yahoo.com (Isabel Soto) Date: Fri, 28 Apr 2017 22:35:38 +0000 (UTC) Subject: [Histonet] cassette and slide printer In-Reply-To: <9a5c802458014db7891d394af67b19e2@SWMS13MAIL12.swmed.org> References: <9a5c802458014db7891d394af67b19e2@SWMS13MAIL12.swmed.org> Message-ID: <1232927278.77023.1493418938846@mail.yahoo.com> Vanage from ventana. It's the best Sent from Yahoo Mail on Android On Fri, Apr 28, 2017 at 2:16 PM, Blanca Lopez via Histonet wrote: Dear Histonettes, I am on my search of looking for a cassette and slide printer for a small lab. Any recommendations are welcome:) Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From isabelsoto1162 at yahoo.com Fri Apr 28 17:35:38 2017 From: isabelsoto1162 at yahoo.com (Isabel Soto) Date: Fri, 28 Apr 2017 22:35:38 +0000 (UTC) Subject: [Histonet] cassette and slide printer In-Reply-To: <9a5c802458014db7891d394af67b19e2@SWMS13MAIL12.swmed.org> References: <9a5c802458014db7891d394af67b19e2@SWMS13MAIL12.swmed.org> Message-ID: <1232927278.77023.1493418938846@mail.yahoo.com> Vanage from ventana. It's the best Sent from Yahoo Mail on Android On Fri, Apr 28, 2017 at 2:16 PM, Blanca Lopez via Histonet wrote: Dear Histonettes, I am on my search of looking for a cassette and slide printer for a small lab. Any recommendations are welcome:) Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet