From rjbuesa at yahoo.com Thu Sep 1 09:21:18 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 1 Sep 2016 14:21:18 +0000 (UTC) Subject: [Histonet] Modified Movat's Pentachrome stain In-Reply-To: References: Message-ID: <501300587.3297908.1472739678347@mail.yahoo.com> Once you start substituting things in an original recipe, the outcome cannot be expected to be what the original recipe was supposed to deliver.Iodine crystals cannot be substituted by Lugol because, besides the iodine also contains its salts. and alcohol.?They are two completely different things. This is the same as using Harris hematoxylin without mercury, you can keep calling it Harris, but it no longer will stain the same. Finally I do not see any advantages of Movat's pentachrome over a good Masson. Just my opinion but I think you are embarking in a complex process with little chance of finishing with the expected results.Ren? On Wednesday, August 31, 2016 9:53 PM, Angela Lamberth via Histonet wrote: Hi Histonetters! I?m gearing up to perform a pentachrome stain. I will be making this in house and not using a kit. Through searching histonet, I?ve found a protocol used by the Children?s Hospital of Philadelphia Pathology Core. http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf Two things:? Iodine crystals are out of the question. Can I replace that with Lugol?s iodine solution or should I just substitute Weigert?s for the hematoxylin in this protocol? Also, saffron vs tartrazine vs Orange G stain.? There is a real difference in price. Does anybody have a personal preference in terms of quality or aesthetics? Is paying the extra money for saffron really worth it? Thanks! Angela -- Angela Lamberth Histology Technician II Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alamberth at lji.org Thu Sep 1 09:39:31 2016 From: alamberth at lji.org (Angela Lamberth) Date: Thu, 1 Sep 2016 07:39:31 -0700 Subject: [Histonet] Modified Movat's Pentachrome stain In-Reply-To: <501300587.3297908.1472739678347@mail.yahoo.com> References: <501300587.3297908.1472739678347@mail.yahoo.com> Message-ID: I would prefer to not substitute anything yet the procedure calls for a minimum of 2 grams of iodine which I simply cannot buy in the United States thanks to new DEA regulations. On Thu, Sep 1, 2016 at 7:21 AM, Rene J Buesa wrote: > Once you start substituting things in an original recipe, the outcome > cannot be expected to be what the original recipe was supposed to deliver. > Iodine crystals cannot be substituted by Lugol because, besides the iodine > also contains its salts. and alcohol. They are two completely different > things. This is the same as using Harris hematoxylin without mercury, you > can keep calling it Harris, but it no longer will stain the same. Finally I > do not see any advantages of Movat's pentachrome over a good Masson. Just > my opinion but I think you are embarking in a complex process with little > chance of finishing with the expected results. > Ren? > > > On Wednesday, August 31, 2016 9:53 PM, Angela Lamberth via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hi Histonetters! > > I?m gearing up to perform a pentachrome stain. I will be making this in > house and not using a kit. Through searching histonet, I?ve found a > protocol used by the Children?s Hospital of Philadelphia Pathology Core. > http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf > > > Two things: Iodine crystals are out of the question. Can I replace that > with Lugol?s iodine solution or should I just substitute Weigert?s for the > hematoxylin in this protocol? > > > Also, saffron vs tartrazine vs Orange G stain. There is a real difference > in price. Does anybody have a personal preference in terms of quality or > aesthetics? Is paying the extra money for saffron really worth it? > > > Thanks! > > Angela > > > > -- > Angela Lamberth > Histology Technician II > Histology Core Lab > La Jolla Institute for Allergy & Immunology > 9420 Athena Circle > La Jolla, CA 92037 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- Angela Lamberth Histology Technician II Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 From rjbuesa at yahoo.com Thu Sep 1 09:47:04 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 1 Sep 2016 14:47:04 +0000 (UTC) Subject: [Histonet] Modified Movat's Pentachrome stain In-Reply-To: References: <501300587.3297908.1472739678347@mail.yahoo.com> Message-ID: <1853685465.3263894.1472741224312@mail.yahoo.com> Do you have any contacts at any old histology lab, i.e., one that has been in operation for more than 50 years? You may find there iodine crystals and ask for a few grams. I used to have a 500 g bottle at my lab (which?began in 1947).Ren? On Thursday, September 1, 2016 10:39 AM, Angela Lamberth wrote: I would prefer to not substitute anything yet the procedure calls for a minimum of 2 grams of iodine which I simply cannot buy in the United States thanks to new DEA regulations. On Thu, Sep 1, 2016 at 7:21 AM, Rene J Buesa wrote: Once you start substituting things in an original recipe, the outcome cannot be expected to be what the original recipe was supposed to deliver.Iodine crystals cannot be substituted by Lugol because, besides the iodine also contains its salts. and alcohol.?They are two completely different things. This is the same as using Harris hematoxylin without mercury, you can keep calling it Harris, but it no longer will stain the same. Finally I do not see any advantages of Movat's pentachrome over a good Masson. Just my opinion but I think you are embarking in a complex process with little chance of finishing with the expected results.Ren? On Wednesday, August 31, 2016 9:53 PM, Angela Lamberth via Histonet wrote: Hi Histonetters! I?m gearing up to perform a pentachrome stain. I will be making this in house and not using a kit. Through searching histonet, I?ve found a protocol used by the Children?s Hospital of Philadelphia Pathology Core. http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf Two things:? Iodine crystals are out of the question. Can I replace that with Lugol?s iodine solution or should I just substitute Weigert?s for the hematoxylin in this protocol? Also, saffron vs tartrazine vs Orange G stain.? There is a real difference in price. Does anybody have a personal preference in terms of quality or aesthetics? Is paying the extra money for saffron really worth it? Thanks! Angela -- Angela Lamberth Histology Technician II Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 ______________________________ _________________ Histonet mailing list mailto:Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Angela LamberthHistology Technician IIHistology Core LabLa Jolla Institute for Allergy & Immunology9420 Athena CircleLa Jolla, CA 92037 From alamberth at lji.org Thu Sep 1 09:58:32 2016 From: alamberth at lji.org (Angela Lamberth) Date: Thu, 1 Sep 2016 07:58:32 -0700 Subject: [Histonet] Modified Movat's Pentachrome stain In-Reply-To: <1853685465.3263894.1472741224312@mail.yahoo.com> References: <501300587.3297908.1472739678347@mail.yahoo.com> <1853685465.3263894.1472741224312@mail.yahoo.com> Message-ID: Thank you for the suggestion. I'll ask around when I reconnect with people at Long Beach this month. On Thu, Sep 1, 2016 at 7:47 AM, Rene J Buesa wrote: > Do you have any contacts at any old histology lab, i.e., one that has been > in operation for more than 50 years? You may find there iodine crystals and > ask for a few grams. I used to have a 500 g bottle at my lab (which began > in 1947). > Ren? > > > On Thursday, September 1, 2016 10:39 AM, Angela Lamberth < > alamberth at lji.org> wrote: > > > I would prefer to not substitute anything yet the procedure calls for a > minimum of 2 grams of iodine which I simply cannot buy in the United States > thanks to new DEA regulations. > > On Thu, Sep 1, 2016 at 7:21 AM, Rene J Buesa wrote: > > Once you start substituting things in an original recipe, the outcome > cannot be expected to be what the original recipe was supposed to deliver. > Iodine crystals cannot be substituted by Lugol because, besides the iodine > also contains its salts. and alcohol. They are two completely different > things. This is the same as using Harris hematoxylin without mercury, you > can keep calling it Harris, but it no longer will stain the same. Finally I > do not see any advantages of Movat's pentachrome over a good Masson. Just > my opinion but I think you are embarking in a complex process with little > chance of finishing with the expected results. > Ren? > > > On Wednesday, August 31, 2016 9:53 PM, Angela Lamberth via Histonet < > mailto:histonet at lists.utsouthwestern.edu > > wrote: > > > Hi Histonetters! > > I?m gearing up to perform a pentachrome stain. I will be making this in > house and not using a kit. Through searching histonet, I?ve found a > protocol used by the Children?s Hospital of Philadelphia Pathology Core. > http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf > > > Two things: Iodine crystals are out of the question. Can I replace that > with Lugol?s iodine solution or should I just substitute Weigert?s for the > hematoxylin in this protocol? > > > Also, saffron vs tartrazine vs Orange G stain. There is a real difference > in price. Does anybody have a personal preference in terms of quality or > aesthetics? Is paying the extra money for saffron really worth it? > > > Thanks! > > Angela > > > > -- > Angela Lamberth > Histology Technician II > Histology Core Lab > La Jolla Institute for Allergy & Immunology > 9420 Athena Circle > La Jolla, CA 92037 > ______________________________ _________________ > Histonet mailing list > mailto:Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Angela Lamberth > Histology Technician II > Histology Core Lab > La Jolla Institute for Allergy & Immunology > 9420 Athena Circle > La Jolla, CA 92037 > > > -- Angela Lamberth Histology Technician II Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 From sbaldwin at mhhcc.org Thu Sep 1 11:02:13 2016 From: sbaldwin at mhhcc.org (Baldwin, Kathy) Date: Thu, 1 Sep 2016 16:02:13 +0000 Subject: [Histonet] FW: NKX 3.1 protocol- In-Reply-To: <75fe636e18684425872bd08e58b7515a@exch02.mhhcc.org> References: <75fe636e18684425872bd08e58b7515a@exch02.mhhcc.org> Message-ID: Hello Histonetters Was wondering if anyone would share their protocol for the above ABY we are utilizing the Ventana Ultra and purchased the ABY 3rd party from Cell Marque I would appreciate the help the insert doesn't have the standard protocol on it like the rest :( just want to be safe. Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 Cell 812-887-3357 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Forest.Blankenship at dchstx.org Thu Sep 1 12:24:13 2016 From: Forest.Blankenship at dchstx.org (Forest Blankenship) Date: Thu, 1 Sep 2016 17:24:13 +0000 Subject: [Histonet] Movat Pentachrome Message-ID: <8D5A5A0BF8A80F43A6E52872E56F523C013A35AF@dchmxdb02.driscoll.dch> Angela, the Movat is a wonderful thing for showing loss of elastic fibers in emphysema and cardiovascular cases. We ran a ton of them in training because our lab/histo school was affiliated with the Texas Heart Institute. Currently at the hospital I work at we use a Kit(Polyscientific) for the VVG part of the stain and Saffron du Galantis for the counterstain. It smells great when gently heated. As far as remarks about a trichrome being able to show the same thing; yeah if you also run a VVG on a serial section to show elastic fibers and you lose the awesomeness that is the Movat. Forest Blankenship, HTL(ASCP) Histology Manager Driscoll Children's Hospital Corpus Christ, TX Disclaimer: This email and its content are confidential and intended solely for the use of the addressee. Please notify the sender if you have received this email in error or simply delete it. From KSimeone at leavittmgt.com Thu Sep 1 12:27:49 2016 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Thu, 1 Sep 2016 17:27:49 +0000 Subject: [Histonet] FT OVERNIGHT HT POSTITION Delray Beach, FL In-Reply-To: <43944B1DBAAC2846B7B9D626B5F1233CC91959E5@vm-email.leavittmgt.com> References: <43944B1DBAAC2846B7B9D626B5F1233CC9194C6C@vm-email.leavittmgt.com>, <43944B1DBAAC2846B7B9D626B5F1233CC91950B2@vm-email.leavittmgt.com>, <43944B1DBAAC2846B7B9D626B5F1233CC91959E5@vm-email.leavittmgt.com> Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CC91962BA@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed HISTOTECHNOLOGIST here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Please fill out employment application HERE http://www.indeed.com/m/viewjob?jk=266fccd1fa688bb6&from=serp ^^you MUST follow this application link to apply! No exceptions. *full time position Mon-Fri OR Sun-Thurs 10p-6:30AM *MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo most of your shift) *MUST have at LEAST FIVE (5) years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE! *VERY proficient in embedding and microtomy *WILL MOSTLY BE EMBEDDING EXCISION BLOCKS, please know DERMS *must be self motivated, reliable and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) *some IHC experience preferred but not necessary Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor Delray Beach Technical Laboratory ADCS Clinics, LLC Advanced Dermatology and Cosmetic Surgery Ameriderm www.advancedderm.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From vcaldwell4232 at gmail.com Thu Sep 1 12:33:46 2016 From: vcaldwell4232 at gmail.com (Van Caldwell) Date: Thu, 1 Sep 2016 10:33:46 -0700 Subject: [Histonet] Position Available Santa Barbara California Area Message-ID: 29/Aug/16 Requisition: 2067718 Job Title: Technical Support Specialist Description: Agilent inspires and supports discoveries that advance the quality of life. We provide life science, diagnostic and applied market laboratories worldwide with instruments, services, consumables, applications and expertise. Agilent enables customers to gain the answers and insights they seek ?? so they can do what they do best: improve the world around us. Information about Agilent is available at www.agilent.com. Diagnostic Services is looking for an experienced technology leader with a strong call center support background with medical device hardware and software to deliver superior support activities for our pathology/histology instrumentation solutions. We develop pathology solutions for tissue-based cancer diagnostics. Our portfolio of solutions includes fully automated systems, all operating in a highly-regulated market environment. We are using lean lab approaches to increase our customer's productivity and efficiency while simultaneously eliminating the chances of operational errors in their workflows. This is an opportunity to join an enthusiastic and dynamic team and work closely with application experts, hardware experts, marketing, QA/RA and directly with customers. Responsibilities include: - Primary responsibility is to provide Post Sales Tier 1 support via the phone to Agilent?s existing customers M-F 6AM to 3PM. - Provides escalation support to internal resources including Tier II Technical Support, Field Service, Customer Service and Application Specialists. - Monitor the Technical Support Help Desk to respond to inquiries and requests for support. - Document all complaints or reportable events per QMS procedures. - Solves complex problems requiring some technical and business admin breadth/ depth of knowledge. Qualifications: - Bachelor?s degree, specialized training/certification, or equivalent combination of education and experience. - 5+ year?s relevant experience in similar position. - Troubleshooting knowledge and skills to complete specialized tasks. - Competent with Microsoft Office (Word, Excel, PowerPoint, Outlook) - Some experience with Laboratory Information Systems, Laboratory Equipment and Laboratory Workflow is a plus. Agilent Technologies, Inc. is an Equal Employment Opportunity and Affirmative Action employer. We value diversity at all levels. All individuals, regardless of personal characteristics, are encouraged to apply. All qualified applicants will receive consideration for employment without regard to sex, pregnancy, race, religion or religious creed, color, gender, gender identity, gender expression, national origin, ancestry, physical or mental disability, medical condition, genetic information, marital status, registered domestic partner status, age, sexual orientation, military or veteran status, protected veteran status, or any other basis protected by federal, state, local law, ordinance, or regulation and will not be discriminated against on these bases. For more information about equal employment opportunity protections, please view the ?EEO is the Law? poster available here: https://www.dol.gov/ofccp/regs/compliance/posters/pdf/eeopost.pdf, https://www.dol.gov/ofccp/regs/compliance/posters/pdf/OFCCP_EEO_Supplement_Final_JRF_QA_508c.pdf Agilent Technologies, Inc., is committed to diversity in the workplace and strives to support candidates with disabilities. If you have a disability and need assistance with any part of the application or interview process or have questions about workplace accessibility, please contact +1-262-754-5030 (US and Canada only) or email job_posting at agilent.com. EOE AA M/F/Vet/Disability Company: Dako Business: Agilent CrossLab Group Job Category: Support / Service Job Sub-Category: Customer Care Tech Region: Americas Country or Area: United States State/Province: California Town/City: Carpinteria Shift: Day Job Job Type: Experienced Schedule: Full-time Travel Required: Yes, 10% of the Time Duration (Temp Positions Only): Not applicable From tbraud at holyredeemer.com Thu Sep 1 12:43:31 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 1 Sep 2016 17:43:31 +0000 Subject: [Histonet] Movat's pentachrome Message-ID: <48E053DDF6CE074DB6A7414BA05403F8087BC4@HRHEX02-HOS.holyredeemer.local> Just wondering why you are compelled to make all the reagents in house for the Movat's stain. I have used the kit k042 from Poly Scientic with fabulous results (and no, I have no gain from recommending them). Their solutions are wonderfully labeled and can be replenished individually, or by ordering the kit. All the time you spend making and labeling and validating "homemade" would offset any cost increase from purchasing their premade solutions. I know. I did the math. Just a suggestion. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 From srishan at mail.holyname.org Thu Sep 1 12:48:41 2016 From: srishan at mail.holyname.org (srishan at mail.holyname.org) Date: Thu, 1 Sep 2016 13:48:41 -0400 Subject: [Histonet] (no subject) Message-ID: Hi, What is everyone doing about the Histology Staff competencies. I know CLIA has several criteria for the med-tech competencies. Since the Histotechs do not report out results, how is everyone implementing this process. Any information is greatly appreciated. Nirmala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From liz at premierlab.com Thu Sep 1 12:59:58 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Thu, 1 Sep 2016 11:59:58 -0600 Subject: [Histonet] Movat Pentachrome In-Reply-To: <8D5A5A0BF8A80F43A6E52872E56F523C013A35AF@dchmxdb02.driscoll.dch> References: <8D5A5A0BF8A80F43A6E52872E56F523C013A35AF@dchmxdb02.driscoll.dch> Message-ID: <14E2C6176416974295479C64A11CB9AE02BEDBE7A507@SBS2K8.premierlab.local> If you are not that concerned over the alcian blue portion of the movats you can perform an elastic trichrome - that's what we do here. Mordant like you would in Bouins, and rather than stain with weigerts hemastoxylin, stain with the elastic portion of the VVG, differentiate like you would for elastic fibers and then continue on with the rest of the trichrome, it turns out great. I have an SOP if anyone wants it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Forest Blankenship via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 01, 2016 11:24 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Movat Pentachrome Angela, the Movat is a wonderful thing for showing loss of elastic fibers in emphysema and cardiovascular cases. We ran a ton of them in training because our lab/histo school was affiliated with the Texas Heart Institute. Currently at the hospital I work at we use a Kit(Polyscientific) for the VVG part of the stain and Saffron du Galantis for the counterstain. It smells great when gently heated. As far as remarks about a trichrome being able to show the same thing; yeah if you also run a VVG on a serial section to show elastic fibers and you lose the awesomeness that is the Movat. Forest Blankenship, HTL(ASCP) Histology Manager Driscoll Children's Hospital Corpus Christ, TX Disclaimer: This email and its content are confidential and intended solely for the use of the addressee. Please notify the sender if you have received this email in error or simply delete it. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Thu Sep 1 13:25:28 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 1 Sep 2016 18:25:28 +0000 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF6FD83A99@ex07.net.ucsf.edu> Nirmala, Histotechs are not required to have competency assessment under CLIA regulations because, as you note, they do not interpret any tests or report any results. However, if they do grossing then they do require high complexity competency assessment. Having said that, if there is some concern in an inspection, any deemed accreditation agency (CLIA, CAP or JC) inspector, or state inspector, can ask how you determine competency of any lab staff member to do any task. It is up to the Laboratory Director to determine how that is done, and how often. An institution may do it differently than the standard CLIA regulations outline, less often and less rigorous, but will need to convince an inspector that it is acceptable. The easiest way is to be sure there will not be any problems is to follow the competency assessment criteria for medium and high complexity - that is accepted and familiar to the inspectors so they should have no problem accepting it for other staff. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Nirmala Srishan via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 01, 2016 10:49 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, What is everyone doing about the Histology Staff competencies. I know CLIA has several criteria for the med-tech competencies. Since the Histotechs do not report out results, how is everyone implementing this process. Any information is greatly appreciated. Nirmala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Thu Sep 1 13:26:48 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 1 Sep 2016 18:26:48 +0000 Subject: [Histonet] Histotech competency evaluation RE: (no subject) Message-ID: <761E2B5697F795489C8710BCC72141FF6FD83AAC@ex07.net.ucsf.edu> Nirmala, Histotechs are not required to have competency assessment under CLIA regulations because, as you note, they do not interpret any tests or report any results. However, if they do grossing then they do require high complexity competency assessment. Having said that, if there is some concern in an inspection, any deemed accreditation agency (CLIA, CAP or JC) inspector, or state inspector, can ask how you determine competency of any lab staff member to do any task. It is up to the Laboratory Director to determine how that is done, and how often. An institution may do it differently than the standard CLIA regulations outline, less often and less rigorous, but will need to convince an inspector that it is acceptable. The easiest way is to be sure there will not be any problems is to follow the competency assessment criteria for medium and high complexity - that is accepted and familiar to the inspectors so they should have no problem accepting it for other staff. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Nirmala Srishan via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 01, 2016 10:49 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, What is everyone doing about the Histology Staff competencies. I know CLIA has several criteria for the med-tech competencies. Since the Histotechs do not report out results, how is everyone implementing this process. Any information is greatly appreciated. Nirmala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Thu Sep 1 13:39:01 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 1 Sep 2016 14:39:01 -0400 Subject: [Histonet] Modified Movat's Pentachrome stain Message-ID: Angela Lamberth at the La Jolla [California] Institute for Allergy & Immunology asks: >>I'm gearing up to perform a pentachrome stain. I will be making this in house and not using a kit. Through searching histonet, I've found a protocol used by the Children?s Hospital of Philadelphia Pathology Core. http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf Two things: Iodine crystals are out of the question. Can I replace that with Lugol's iodine solution or should I just substitute Weigert's for the hematoxylin in this protocol? Also, saffron vs tartrazine vs Orange G stain? There is a real difference in price. Does anybody have a personal preference in terms of quality or aesthetics? Is paying the extra money for saffron really worth it?<< THE expert on the Movat pentachrome stain is Akemi Allison, a very senior histotechnologist who's published about it. I can't remember if she's on Histonet, but you can contact her on Facebook - I hear from her there quite often. I'm not personally familiar with the stain, but I don't think either of those substitutions will work. You need to find out more about saffron before you work with it, because it's so expensive. Bob Richmond Samurai Pathologist Maryville TN From ADuddey at firsthealth.org Thu Sep 1 13:39:11 2016 From: ADuddey at firsthealth.org (Duddey, Aimee) Date: Thu, 1 Sep 2016 18:39:11 +0000 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <09FBA01CA9B6374A83C5C76E09E46188A34A8999@EXMAIL1-FHC.firsthealth.org> We ran into this situation with TJC. We had a very limited check off sheet. In effort to standardize processes across the laboratory we adopted a program very similar to the NY facility whose program is referenced in the TJC leading practice library. It is very rigorous and time consuming but serves its purpose well. Also, CAP has a competency assessment program specifically for histology that is a subscription based purchase regardless if you are accredited by them. Aimee -----Original Message----- From: Nirmala Srishan via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 01, 2016 1:49 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, What is everyone doing about the Histology Staff competencies. I know CLIA has several criteria for the med-tech competencies. Since the Histotechs do not report out results, how is everyone implementing this process. Any information is greatly appreciated. Nirmala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AJohnson at aipathology.com Thu Sep 1 14:24:58 2016 From: AJohnson at aipathology.com (Amy Johnson) Date: Thu, 1 Sep 2016 19:24:58 +0000 Subject: [Histonet] H. pylori stain Message-ID: Hello histoland We currently use Ventana's h. pylori antibody on our Benchmark ultra. Over the last few months our pathologists have been noticing "junk" that is staining with this antibody. It seems to be on the tissue more than in the tissue and its not on all slides. WE have tried changing our waterbath from tap water to distilled water with no difference in staining. We thought maybe our paraffin was bad so we stained a "blank" block of paraffin but could not find the culprit.......Any insight would be appreciated! Thanks Amylin Johnson, B.S. HTL(ASCP) Associates in Pathology Wausau Wi 54401 715-847-2130 From amosbrooks at gmail.com Thu Sep 1 14:35:11 2016 From: amosbrooks at gmail.com (Amos Brooks) Date: Thu, 1 Sep 2016 15:35:11 -0400 Subject: [Histonet] Modified Pentachrome In-Reply-To: References: Message-ID: Hi, I use Lugol's Iodine all the time for this. It works just fine. I do purchase certain chemicals from manufacturers like Lugol's Iodine which I get from EMS. Most of the chemicals I make up myself from powder though. I have shared the procedure with you via Google Docs. I hope it helps. Amos From alamberth at lji.org Thu Sep 1 18:18:48 2016 From: alamberth at lji.org (Angela Lamberth) Date: Thu, 1 Sep 2016 16:18:48 -0700 Subject: [Histonet] Modified Pentachrome In-Reply-To: References: Message-ID: Thanks for sharing. It's very helpful. Where do you purchase your safran du gatinais/saffron from? At this point I'm ready to start growing my own Crocus sativus. On Thu, Sep 1, 2016 at 12:35 PM, Amos Brooks wrote: > Hi, > I use Lugol's Iodine all the time for this. It works just fine. I do > purchase certain chemicals from manufacturers like Lugol's Iodine which I > get from EMS. Most of the chemicals I make up myself from powder though. I > have shared the procedure with you via Google Docs. I hope it helps. > > Amos > -- Angela Lamberth Histology Technician II Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 From alamberth at lji.org Thu Sep 1 19:06:06 2016 From: alamberth at lji.org (Angela Lamberth) Date: Thu, 1 Sep 2016 17:06:06 -0700 Subject: [Histonet] Movat's pentachrome Message-ID: Hi Terri, I have used Poly Scientific kits in the past and agree that they are fabulous. If I were still in anatomic pathology I would wholeheartedly agree with you. However, I'm now in a research facility and the kit is simply cost prohibitive. Fortunately, I do now have time for homemade (a true luxury that I acknowledge is not applicable in AP). Thank you for your suggestion. :) PS: This is my first time replying to a post on histonet that did not come to my email inbox. Did I do it right?? Just wondering why you are compelled to make all the reagents in house for the Movat's stain. I have used the kit k042 from Poly Scientic with fabulous results (and no, I have no gain from recommending them). Their solutions are wonderfully labeled and can be replenished individually, or by ordering the kit. All the time you spend making and labeling and validating "homemade" would offset any cost increase from purchasing their premade solutions. I know. I did the math. Just a suggestion. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 -- Angela Lamberth Histology Technician II Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 From alamberth at lji.org Thu Sep 1 21:11:32 2016 From: alamberth at lji.org (Angela Lamberth) Date: Thu, 1 Sep 2016 19:11:32 -0700 Subject: [Histonet] Movat's pentachrome Message-ID: Thanks Bob. Somebody else mentioned her name also so I'll be sure to reach out to her. THE expert on the Movat pentachrome stain is Akemi Allison, a very senior histotechnologist who's published about it. I can't remember if she's on Histonet, but you can contact her on Facebook - I hear from her there quite often. I'm not personally familiar with the stain, but I don't think either of those substitutions will work. You need to find out more about saffron before you work with it, because it's so expensive. Bob Richmond Samurai Pathologist Maryville TN -- Angela Lamberth Histology Technician II Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 From ADuddey at firsthealth.org Fri Sep 2 07:23:01 2016 From: ADuddey at firsthealth.org (Duddey, Aimee) Date: Fri, 2 Sep 2016 12:23:01 +0000 Subject: [Histonet] H. pylori stain In-Reply-To: References: Message-ID: <09FBA01CA9B6374A83C5C76E09E46188A34A8BA6@EXMAIL1-FHC.firsthealth.org> We have BMK Ultras for years. I would suggest running the decontam for the stainer with the prescribed Lysol IC solution and then see what you get. It the trash in your control tissue too or just the patient tissue? Aimee -----Original Message----- From: Amy Johnson via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 01, 2016 3:25 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] H. pylori stain Hello histoland We currently use Ventana's h. pylori antibody on our Benchmark ultra. Over the last few months our pathologists have been noticing "junk" that is staining with this antibody. It seems to be on the tissue more than in the tissue and its not on all slides. WE have tried changing our waterbath from tap water to distilled water with no difference in staining. We thought maybe our paraffin was bad so we stained a "blank" block of paraffin but could not find the culprit.......Any insight would be appreciated! Thanks Amylin Johnson, B.S. HTL(ASCP) Associates in Pathology Wausau Wi 54401 715-847-2130 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MMargiotta at bmhmc.org Fri Sep 2 13:46:34 2016 From: MMargiotta at bmhmc.org (Margiotta-Watz, Michele) Date: Fri, 2 Sep 2016 18:46:34 +0000 Subject: [Histonet] test Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC6DA31FA7@BMH-EXCHANGE-01.BMHMC.ORG> test Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Road Patchogue, NY 11772 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From JMacDonald at mtsac.edu Fri Sep 2 13:54:40 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Fri, 2 Sep 2016 11:54:40 -0700 Subject: [Histonet] Nurses in charge of labs Message-ID: This decision could affect histology labs as well. Please read below and consider the effects of having a nurse in charge of the lab. Jennifer Petition Urging CMS to Reconsider Ruling on Nursing Degree Equivalence ASCLS, in conjunction with our partners on the Board of Certification, urge the laboratory community and other interested individuals to Sign the Petition urging the Centers for Medicare and Medicaid Services (CMS) to reconsider its position that nursing is a biological science for purposes of performing laboratory testing. [ASCLS_Logo] Government Affairs Alert Sign Petition Urging CMS to Reconsider Ruling on Nursing Degree Equivalence ASCLS, in conjunction with our partners on the Board of Certification, urge the laboratory community and other interested individuals to Sign the Petition urging the Centers for Medicare & Medicaid Services (CMS) to reconsider its position that nursing is a biological science for purposes of performing laboratory testing. Sign the petition here.< http://weblaunch.blifax.com/listener3/redirect?l=8b3397a5-c6e1-4643-a69c-00f119b9c805&id=ca0c8eaf-ab6f-e611-95c6-0050569f409f&u=http%3a%2f%2fcqrcengage.com%2fascpath%2fapp%2fsign-petition%3f0%26engagementId%3d239813 > On April 1, CMS announced that ?an associate?s or bachelor?s degree in nursing is equivalent to an associate?s or bachelor?s degree, respectively, in biological science??seemingly declaring that individuals with a nursing degree are potentially as qualified to perform advanced testing as certified laboratory professionals. It also appears that CMS?s position could allow individuals with as little as a bachelor?s degree in nursing to direct a CLIA moderate complexity laboratory and/or serve in senior supervisory roles within a CLIA high complexity laboratory. Since the Clinical Laboratory Improvement Amendments (CLIA) of 1988 doesn?t specifically require clinical training of individuals with a degree in biological sciences, CMS?s new policy exempts individuals with a bachelor?s degree in nursing from any specific training requirement prior to performing high complexity testing for diagnostic purposes. We have great respect for the work and invaluable services nurses provide patients, but we do not agree that the nursing degree is equivalent to a biological sciences degree or that it would adequately prepare someone to perform non-waived laboratory services. Our greatest concern is this policy will negatively affect test quality and patient outcomes as well as effect access to quality testing services. Take a few minutes to Sign the Petition to tell CMS< http://weblaunch.blifax.com/listener3/redirect?l=cbcef357-c5ab-48dd-87f6-761b108f6837&id=ca0c8eaf-ab6f-e611-95c6-0050569f409f&u=http%3a%2f%2fcqrcengage.com%2fascpath%2fapp%2fsign-petition%3f0%26engagementId%3d239813 > that you believe a degree in nursing is not the same thing as a degree in the biological sciences and that appropriate academic coursework and clinical training/experience are need to provide quality testing services. If you have any questions, please direct them to ASCLS Executive Vice President Jim Flanigan via email at jimf at ascls.org. [ http://images.blifax.com/images/logos/BriCho5694/filelib_978696/ascls_SQR_74.jpg ] American Society for Clinical Laboratory Science 1861 International Drive, Suite 200 McLean, Virginia USA 22102 +1.571.748.3770 www.ascls.org If you would like to forward this message to someone else, please Click Here< http://weblaunch.blifax.com/listener3/forward?id=ca0c8eaf-ab6f-e611-95c6-0050569f409f&e=ebeitz at wellspan.org >. 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If you are not the intended recipient, you are hereby notified that any use, disclosure, dissemination, distribution, forwarding, or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender by reply Fax or e-mail stating the communication was "received in error" and delete or destroy all copies of this communication, including all attachments. Message string: KqxfH=TU oo-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-oo TIPS: http://www.ualberta.ca/~pletendr/clseduc.html Post a question: E-mail Contact listowners: E-mail ARCHIVE Log-in: http://list.apsu.edu/scripts/wa-APSU.exe?A0=CLSEDUC oo-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-oo From bhartologist at gmail.com Fri Sep 2 16:48:23 2016 From: bhartologist at gmail.com (Bharti Parihar) Date: Fri, 2 Sep 2016 14:48:23 -0700 Subject: [Histonet] Histology Tech Position available at Kaiser Permanente Berkeley Regional Lab! Message-ID: Hello community! If anyone is interested in moving to beautiful California or you're already in the area and you'd like to join Kaiser Permanente, there is 12:00 a.m. to 8:30 a.m. position available for licensed HTs in the Berkeley area! I'm more than happy to help anyone interested to get on board! If the hours aren't your first choice I understand. But all hope isn't lost! I started with the company at that shift time and after about 6 months an earlier shift opened up and with the power of being a certified tech and seniority, I was able to get the shift. I've been able to move to an even earlier shift since then as well, so no worries! Feel free to respond if anyone is interested! Take care! -- Bharti Parihar, HT (ASCP)CM From Karoleigh.Armstrong at ARMC.net Fri Sep 2 16:59:40 2016 From: Karoleigh.Armstrong at ARMC.net (Armstrong, Karoleigh T) Date: Fri, 2 Sep 2016 21:59:40 +0000 Subject: [Histonet] Job opening Message-ID: Hello Histo Friends. Abilene Regional Medical Center, in Abilene Texas, has a job opening for a Histotech, H.T. or H.T.L. (ASCP), or eligble. ARMC is a 150+ bed hospital in Central Texas. Morning hours Monday- Friday, many Holidays off. Contact ARMC.net for application -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From SteveM at mcclainlab.com Sat Sep 3 05:15:10 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Sat, 3 Sep 2016 10:15:10 +0000 Subject: [Histonet] Histonet Digest, Vol 154, Issue 2 movats In-Reply-To: References: Message-ID: Liz, please send SOP ASAP! The vvg is a hematoxylin-that's why it works so well. The problem w Movat is the photography. It is complex w 5 color signals, hard to show all 5 in one image. Liz, What control did you use? Steve A. McClain, MD > On Sep 2, 2016, at 13:13, "histonet-request at lists.utsouthwestern.edu" wrote: > > weigerts hemastoxylin, stain with the elastic portion of the VVG, differentiate like you would for elastic fibers and then continue on with the rest of the trichrome, it turns out great. I have an SOP if anyone wants it. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC From amosbrooks at gmail.com Sat Sep 3 07:37:59 2016 From: amosbrooks at gmail.com (Amos Brooks) Date: Sat, 3 Sep 2016 08:37:59 -0400 Subject: [Histonet] Modified Pentachrome In-Reply-To: References: Message-ID: Hi, I totally forgot to get the info about the safran Friday. I'll find it when I get back on Tuesday. I just didn't want you to think I had forgotten about you. A note about the safran; It is an anhydrous solution and upon making it up one would think it was goofed up because most of the safran does not dissolve. There is a reservoir of undissolved solute in the bottom of the container. Filter off what you need (without resuspending it) and dump it back to re-use. The solution lasts a really long time. If you are hesitant about making any of the chemicals, I am sure Poly Scientific, American Mastertech or one of the many other manufacturers would supply it. This is probably the least hazardous chemical you will use in histology though. Short of the 100% ETOH used, it is the same stuff you would use to cook with and as Rob Richmond correctly states it smells delightful. By the way, good on you for making up your own chemicals. It is *much* less expensive. Having done the math on this for tris buffered saline, most manufacturers mark up the price literally 400%! For water... Really! The first time you make something up it takes a bit of time making sure things are going right. After that it is routine and is quick and efficient reducing the labor cost factor. Thank you for keeping histology real :-) Amos On Sep 1, 2016 7:19 PM, "Angela Lamberth" wrote: Thanks for sharing. It's very helpful. Where do you purchase your safran du gatinais/saffron from? At this point I'm ready to start growing my own Crocus sativus. On Thu, Sep 1, 2016 at 12:35 PM, Amos Brooks wrote: > Hi, > I use Lugol's Iodine all the time for this. It works just fine. I do > purchase certain chemicals from manufacturers like Lugol's Iodine which I > get from EMS. Most of the chemicals I make up myself from powder though. I > have shared the procedure with you via Google Docs. I hope it helps. > > Amos > -- Angela Lamberth Histology Technician II Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 From susanbachus at verizon.net Sat Sep 3 12:12:33 2016 From: susanbachus at verizon.net (susanbachus at verizon.net) Date: Sat, 03 Sep 2016 13:12:33 -0400 Subject: [Histonet] looking for The Art of Histology by Stephanie De Ritis, Sept. 6, 2004, Advance In-Reply-To: References: <000001ca225c$ee91f940$cbb5ebc0$@net> <263168.48894.qm@web46104.mail.sp1.yahoo.com> Message-ID: <9B5B42A1612544E98A099C7722FBB37E@UserPC> I'm trying to find a pdf of this paper for my students. I have an ancient battered xerox copy in shades of gray. For some strange reason it's not in the Advance Medical Library Professionals online archives. I'm hoping that Stephanie is in Histonet, or that someone else has access to a copy. My students thank you! gratefully, Susan From susanbachus at verizon.net Sat Sep 3 12:42:02 2016 From: susanbachus at verizon.net (susanbachus at verizon.net) Date: Sat, 03 Sep 2016 13:42:02 -0400 Subject: [Histonet] looking for The Art of Histology by Stephanie De Ritis, Sept. 6, 2004, Advance In-Reply-To: <9B5B42A1612544E98A099C7722FBB37E@UserPC> References: <000001ca225c$ee91f940$cbb5ebc0$@net> <263168.48894.qm@web46104.mail.sp1.yahoo.com> <9B5B42A1612544E98A099C7722FBB37E@UserPC> Message-ID: <88B830CECBCA4792B4353D70792E6403@UserPC> Sorry, Freudian slip, of course I meant Advance for Medical LABORATORY Professionals! Sometimes my "hats" get mixed up... thanks, Susan -----Original Message----- From: Susan Bachus via Histonet Sent: Saturday, September 03, 2016 1:12 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] looking for The Art of Histology by Stephanie De Ritis, Sept. 6, 2004, Advance I'm trying to find a pdf of this paper for my students. I have an ancient battered xerox copy in shades of gray. For some strange reason it's not in the Advance Medical Library Professionals online archives. I'm hoping that Stephanie is in Histonet, or that someone else has access to a copy. My students thank you! gratefully, Susan _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jriggleman at globusmedical.com Tue Sep 6 15:08:48 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Tue, 6 Sep 2016 20:08:48 +0000 Subject: [Histonet] New Bone vs. Old Bone Stain Message-ID: Hello, Can anyone recommend a stain that differentiates new (woven) bone vs. old (lamellar) bone? We are looking for bone growth, and biomaterial was used to facilitate. We are using a rabbit femoral defect and a rabbit PLF model. Protocols/Input for MMA thin/thick sections and formalin fixed paraffin embedded specimens would be greatly appreciated. Thank you, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From hiratama at kuhp.kyoto-u.ac.jp Tue Sep 6 23:30:14 2016 From: hiratama at kuhp.kyoto-u.ac.jp (=?UTF-8?B?5bmz55Sw5Yud5ZWTKEhpcmF0YU1hc2FoaXJvKQ==?=) Date: Wed, 7 Sep 2016 13:30:14 +0900 Subject: [Histonet] Modified Movat's Pentachrome stain Message-ID: Angela, I replace saffron with Gardenia Fruits Extract (Tokyo Chemical Industry, TCI America in US, C1527) for Movat's and HES. It has no C.I. number, but contains crocin dye (CAS 42553-65-1) ?extract the dye with ethanol (5-6g/100ml) for 2-3days and filtrate. I hope this will help ? for you Masahiro HIRATA Department of Diagnostic Pathology, Kyoto University Hospital Kyoto, JAPAN hiratama at kuhp.kyoto-u.ac.jp 2016-09-02 2:00 GMT+09:00 : > Message: 7 > Date: Wed, 31 Aug 2016 18:24:15 -0700 > From: Angela Lamberth > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Modified Movat's Pentachrome stain > Message-ID: > ail.com> > Content-Type: text/plain; charset=UTF-8 > > Hi Histonetters! > > I?m gearing up to perform a pentachrome stain. I will be making this in > house and not using a kit. Through searching histonet, I?ve found a > protocol used by the Children?s Hospital of Philadelphia Pathology Core. > http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf > > > Two things: Iodine crystals are out of the question. Can I replace that > with Lugol?s iodine solution or should I just substitute Weigert?s for the > hematoxylin in this protocol? > > > Also, saffron vs tartrazine vs Orange G stain. There is a real difference > in price. Does anybody have a personal preference in terms of quality or > aesthetics? Is paying the extra money for saffron really worth it? > > > Thanks! > > Angela > ============================= ???? (Hirata Masahiro) ???????????????? 075-751-3491 hiratama at kuhp.kyoto-u.ac.jp From Blanca.Lopez at UTSouthwestern.edu Wed Sep 7 10:17:29 2016 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Wed, 7 Sep 2016 15:17:29 +0000 Subject: [Histonet] frozen in mouse tissues Message-ID: <590a4f911f50439d9db17f41287eb4cb@SWMS13MAIL12.swmed.org> Hello Histonets, I am looking to solve this issue: what we usually do for froze section is embedded tissue in OCT and then do the sectioning. It works well for other tissues. However, because lung is hollow inside, when I used the same approach (just embed in OCT), the entire lung collapse during sectioning and I cannot even get tissue slides. I checked online and other people say I need to perfuse lung with PBS:OCT 1:1 buffer before embedded into OCT, so the lung will be filled by OCT. I tried this method, and finally I was able to get lung sliced. However, the morphology is bad. Please check the figure below. The lung tissue sections stained with hematoxylin alone (the spleen sections are fine, it's stained with anti-CD4 and hematoxylin). I heard some people also say a prior fixation by formalin before OCT embedding will help to get better morphology Can somebody help? Thanks Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From kdwyatt at vet.k-state.edu Wed Sep 7 10:32:18 2016 From: kdwyatt at vet.k-state.edu (Kristina Wyatt) Date: Wed, 7 Sep 2016 15:32:18 +0000 Subject: [Histonet] working manual Jones methenanmine silver stain Message-ID: Hello, Does anyone have a good procedure for a manual Jones stain, preferably microwave? Thanks, Kristina Wyatt, M.A. Supervisor: Histopathology/Immunohistochemistry L216 Mosier Hall Kansas State Veterinary Diagnostic Laboratory 785-532-4464 kdwyatt at vet.k-state.edu From mward at wakehealth.edu Wed Sep 7 13:34:55 2016 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Wed, 7 Sep 2016 18:34:55 +0000 Subject: [Histonet] Dako Herceptest Link kit question Message-ID: We just installed a new Dako Link Autostainer and have started using the Herceptest Link kit. We had been using two drop zones with our other, older instrument but that was applying only 100 uL in the 2 drop zones. With this new program we set it up to apply two zones of 200 uL but discovered that we will be cutting our test capability in half (50 to about 25 slides). How are other users handling this? Do you use two drop zones, one on each end to cover the control and case material or do you have the reagents apply in one drop zone in the middle of the slide? I am just concerned about the reagents spreading evenly across the slide. What is everyone else's experience with this issue? Thanks in advance for your help! Martha Ward Wake Forest Baptist Health From taynay143 at hotmail.com Thu Sep 8 10:32:32 2016 From: taynay143 at hotmail.com (Tyra Connor) Date: Thu, 8 Sep 2016 15:32:32 +0000 Subject: [Histonet] Starting immunos Message-ID: Hello All, My lab is CAP certified, however this does not include the upstairs Mohs lab. Now they have expressed an interest in doing a MART-1. The two Mohs techs are not HT/HTL, they are OTJ. I am a HT, but was pretty sure that it does not cover me doing immunos. Does the Mohs section have to be CAP accredited to do immunos? Things I do know (admittedly limited): -immunos are not FDA regulated -immunos require basically a Millipore system for reliable staining -possibly would need to purchase an immunostainer (Bond/Ventana/Dako)-again for reliability, I know they can be hand-stained -validations must be done on the stain and the instrument (how many cases/slides and do you send them to someone else for peer review) Basically any advice from you all about a lab that has never done immunos with a doctor that is asking about bringing it online. Thanks for imparting all your vast wisdom, T. From sadrew at wisc.edu Thu Sep 8 12:57:38 2016 From: sadrew at wisc.edu (SALLY A DREW) Date: Thu, 08 Sep 2016 17:57:38 +0000 Subject: [Histonet] Lifespan of slide labeling equipment Message-ID: I'd appreciate people's views on slide labelling equipment for a small lab, especially what folk's feel is an average lifespan. Also, how many people carry maintenance contracts(past the warranty period) on the equipment. Thank you very much for any and all responses! Sally Ann Drew, MT(ASCP) TRIP Lab Manager (TSB-TRIP) 600 Highland Ave. L5/181 CSC Madison, WI 53792-8550 From MMargiotta at bmhmc.org Thu Sep 8 13:29:25 2016 From: MMargiotta at bmhmc.org (Margiotta-Watz, Michele) Date: Thu, 8 Sep 2016 18:29:25 +0000 Subject: [Histonet] implants Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC6DA324A0@BMH-EXCHANGE-01.BMHMC.ORG> Hi All, My Pathologist is questioning whether we still need to save the removed breast implants, and if so, for how long. Looking to see what other hospitals are doing. Thanks, Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Road Patchogue, NY 11772 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From garethdavisyuma at gmail.com Thu Sep 8 14:10:03 2016 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Thu, 8 Sep 2016 12:10:03 -0700 Subject: [Histonet] Ventana Ultra vs. Leica Bond Max Message-ID: I was interested in anyone's opinion on the new Ventana Benchmark Ultra and Leica's Bond Max. I currently use the Ventana Benchmark XT. Ventana wants us to upgrade to the Ultra. Because our contract will be up in a year, my manager and lab owners want me to check out other options. Hoping to get a better deal and a better instrument. The Benchmark XT slide heaters keep going out and the H. pylori is awful, Ventana swears it's the instruments fault. Thanks, -- Ms. Gareth B. Davis, HT, QIHC (ASCPcm) Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From Kim.Kolman at va.gov Thu Sep 8 15:04:52 2016 From: Kim.Kolman at va.gov (Kolman, Kimberly D.) Date: Thu, 8 Sep 2016 15:04:52 -0500 Subject: [Histonet] fixing fatty skin specimens Message-ID: <9C32F30B6662D74A8419DDDB7E66656A0D17BE1D@VHAV15MSGA1.v15.med.va.gov> Does anyone out there have any tips for getting large fatty skin excisions formalin fixed for gross dissection in a reasonable amount of time? Thank you. Kim Kolman Kimberly D. Kolman, HT, (ASCP) VA Eastern Kansas Health Care System Dwight D. Eisenhower VA Medical Center Histology - 115 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 913-682-2000x52537 Fax: 913-758-4193 Kim.kolman at va.gov From Scott.Lindrud at rice.willmar.mn.us Fri Sep 9 07:39:47 2016 From: Scott.Lindrud at rice.willmar.mn.us (Lindrud, Scott) Date: Fri, 9 Sep 2016 12:39:47 +0000 Subject: [Histonet] Decal times for Bone Marrow Biopsies Message-ID: <32e51baa2f2c4bc59695e6d3a372e088@RMH-MAIL.ricehospital.local> Happy Friday to Everyone, I have a question about decal for Trephine collected bone marrows. We currently decal our Trephine collected bone marrow biopsies for 1 hour in Cal-Rite by Richard Allan, a mixture of formic acid and formaldehyde. Although this seems to work adequately most of the time, there are times when the decal isn't as thorough as we would like. My questions for everyone are (if you are willing to share): 1. What do you decal your bone marrows with and for how long? 2. Are you able to perform Fluorescence In Situ Hybridization (FISH) or Chromogenic In Situ Hybridization (CISH) testing on your biopsies with your decal protocol? Thanks for any information you are willing to share! Scott Lindrud Scott A. Lindrud, MLS(ASCP)CM CTCM Histopathology Technical Specialist/Cytotechnologist Rice Memorial Hospital 301 Becker Ave SW Willmar, MN 56201 WP(320)231-4406 Fax(320)231-4861 scott.lindrud at rice.willmar.mn.us ________________________________ "This e-mail transmission is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution violates confidentiality and privacy laws and is prohibited. If you are not the intended recipient, please notify the sender immediately and delete all copies of the message. Thank you." From taynay143 at hotmail.com Fri Sep 9 09:54:17 2016 From: taynay143 at hotmail.com (Tyra Connor) Date: Fri, 9 Sep 2016 14:54:17 +0000 Subject: [Histonet] Starting immunos Message-ID: Thanks to all who responded! I now have starting points for discussion with the docs. Have a good weekend everyone!! T. From Karoleigh.Armstrong at ARMC.net Fri Sep 9 14:42:07 2016 From: Karoleigh.Armstrong at ARMC.net (Armstrong, Karoleigh T) Date: Fri, 9 Sep 2016 19:42:07 +0000 Subject: [Histonet] Job opening Message-ID: Hey gang, We have a Job opening for a H.T. or H.L.T. here in Abilene Tx. Weekdays, weekends and most Holidays off. Abilene has 3 universities, and 2 colleges, a livley art scene. Low cost of living, and the home of the 10 minute rush hour. Contact: ARMC.net and look in employment. Thanks, KK Armstrong H. T. -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From c.tague at Pathologyarts.com Fri Sep 9 16:23:53 2016 From: c.tague at Pathologyarts.com (Curt) Date: Fri, 9 Sep 2016 21:23:53 +0000 Subject: [Histonet] Ventana Ultra vs. Leica Bond Max In-Reply-To: References: Message-ID: <9C8F910F72893643B3C3793C3D67132B67D37919@PATHOLOGYSERVER.pathologyarts.local> Hello, We had Leica Bond Max instruments (2 Bonds) for about 5 years and made the move over the Ventana Ultras (3 Ultras) a couple years ago. We made the move initially for the speed and menu benefits versus the Leica, (Ultra is about 2x faster) but also saw a quality improvement once we tweaked the protocols a bit. I don't know about the difference between the XT and the Ultra because we never had the XT, but from the Leica to the Ultra the biggest improvement was speed. We also saw dirty H. Pylori staining at first with both Leica and Ventana but have cleaned it up dramatically with some processing and fixation changes. I don't know if the instrument has much to do with the staining quality as the changes in our process because the staining was improved using the same Ultra instrument. If you like I would be willing stain your tissue on our instruments to see if you do in fact have a bad instrument??? I can also share with you our protocols to see if that helps your staining issues. Thanks, Curt -----Original Message----- From: Gareth Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 08, 2016 12:10 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Ventana Ultra vs. Leica Bond Max I was interested in anyone's opinion on the new Ventana Benchmark Ultra and Leica's Bond Max. I currently use the Ventana Benchmark XT. Ventana wants us to upgrade to the Ultra. Because our contract will be up in a year, my manager and lab owners want me to check out other options. Hoping to get a better deal and a better instrument. The Benchmark XT slide heaters keep going out and the H. pylori is awful, Ventana swears it's the instruments fault. Thanks, -- Ms. Gareth B. Davis, HT, QIHC (ASCPcm) Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SteveM at mcclainlab.com Sat Sep 10 06:19:34 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Sat, 10 Sep 2016 11:19:34 +0000 Subject: [Histonet] Histonet Digest, Vol 154, Issue 7ubject: fixing fatty skin specimens In-Reply-To: References: Message-ID: <359E4674-08B8-43D4-AB4B-09240814F2CA@mcclainlab.com> Not sure I understand your question. But assume you are looking for margins on a re-excision of cancer or melanoma. Are you using a sufficient -20 times volume of clean 10% nbf? And changing it often? Switch to 20% nbf Or switch to 100% formaldehyde! True dat- Stanford's Dr. McNeal fixed prostates ~6 hours in 37% raw unbuffered in 1986. (We also used Mercury B5 in 1986) The question is whether what are you studying (IHC, PCR ) will stand up to additional fixation or post fixation methods treatment. My plan A approach is to use plenty of clean 10-20% formalin and change it every 6-24hours. Once it firms up, gross and bread loaf slice and get into 2x3 INCH teabags. lay those out for another 3-12-24 hours in 70% alcohol, then process teabags for 12-16-18 hours ROOM TEMPs program 3. heat fixation- I've seen processor demos where microwave cooked tissues processed well and according to the rep 'cut like butter' but 12 years ago I could not photograph the results. We've had decent results w 1 hour RT Carnoy's postfixation in some samples. Love this stuff. It firms up fat, fixes mucin. It can over harden tissue (not in 1hr) and causes shrinkage. But DO NOT HEAT Carnoy's !!! I read it generates phosgene gas. From jriggleman at globusmedical.com Mon Sep 12 09:05:24 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Mon, 12 Sep 2016 14:05:24 +0000 Subject: [Histonet] Egg Whites and IHC Staining Message-ID: Hello, We are cutting large Rabbit PLF formalin fixed paraffin embedded sections. We have had issues staining H&E and Goldner?s Trichrome. Egg whites is working as a great slide adhesive for these two, but not so much for IHC Staining (RAM 11 specifically). Any ideas/suggestions on how to get these sections to adhere? We have tried many methods, such as positively charged slides, chrom-alum gel, and so on. Thank you for all input, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From rjbuesa at yahoo.com Mon Sep 12 09:59:09 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 12 Sep 2016 14:59:09 +0000 (UTC) Subject: [Histonet] Egg Whites and IHC Staining In-Reply-To: References: Message-ID: <636057593.944028.1473692349194@mail.yahoo.com> Egg white (in Mueller's albumin) will always produce a "shadowy" staining with IH procedures y a dark background with IHC procedures.I suggest you use pork gelatin dissolves in the water of the water bath, use (+) charged slides and increase the drying time in the oven.Also sectioning those large pieces as thin as possible will help.Ren? On Monday, September 12, 2016 10:25 AM, Jessica Riggleman via Histonet wrote: Hello, We are cutting large Rabbit PLF formalin fixed paraffin embedded sections. We have had issues staining H&E and Goldner?s Trichrome. Egg whites is working as a great slide adhesive for these two, but not so much for IHC Staining (RAM 11 specifically). Any ideas/suggestions on how to get these sections to adhere? We have tried many methods, such as positively charged slides, chrom-alum gel, and so on. Thank you for all input, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note:? This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwerdler at gmail.com Mon Sep 12 10:08:00 2016 From: mwerdler at gmail.com (Mca Werdler) Date: Mon, 12 Sep 2016 10:08:00 -0500 Subject: [Histonet] Egg Whites and IHC Staining In-Reply-To: References: Message-ID: I am also very interested in this! please let me know a good adhesive for IHC 2016-09-12 9:05 GMT-05:00 Jessica Riggleman via Histonet < histonet at lists.utsouthwestern.edu>: > Hello, > > We are cutting large Rabbit PLF formalin fixed paraffin embedded sections. > > We have had issues staining H&E and Goldner?s Trichrome. Egg whites is > working as a great slide adhesive for these two, but not so much for IHC > Staining (RAM 11 specifically). > > Any ideas/suggestions on how to get these sections to adhere? We have > tried many methods, such as positively charged slides, chrom-alum gel, and > so on. > > Thank you for all input, > Jessica > > > _____________________________________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > > Confidentiality Note: This email is confidential and intended solely for > the use of the individual to whom it is addressed. If you are not the > intended recipient, be advised that you have received this email in error > and that any use, dissemination, forwarding, printing, or copying of this > email is strictly prohibited. If you have received this email in error > please contact the sender. Any views or opinions presented are solely those > of the author and do not necessarily represent those of Globus Medical, > Inc. Although this email and any attachments are believed to be free of any > virus or other defects which might affect any computer or IT system into > which they are received, no responsibility is accepted by Globus Medical, > Inc. for any loss or damage arising in any way from the receipt or use > thereof. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kkienitz at orclinic.com Mon Sep 12 10:42:14 2016 From: kkienitz at orclinic.com (Kienitz, Kari) Date: Mon, 12 Sep 2016 15:42:14 +0000 Subject: [Histonet] patient identifiers Message-ID: Good morning everyone, Along with an accession number, is a patients intitials an adequate identifier on a block/slide? Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz at orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you From Karoleigh.Armstrong at ARMC.net Mon Sep 12 10:42:33 2016 From: Karoleigh.Armstrong at ARMC.net (Armstrong, Karoleigh T) Date: Mon, 12 Sep 2016 15:42:33 +0000 Subject: [Histonet] Job opening Message-ID: Carl, you are so right, I do mean an H.T.L., by Friday afternoon, mommy's brain is cooked! Thanks. Jay, I really am looking for full time. After over 30 years, I am getting ready to retire, in about a year or two, and I want to train someone to take my place as Supervisor. This is a great Lab, and the people are fabulous. Thanks for the interest> KK -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From liz at premierlab.com Mon Sep 12 10:53:03 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Mon, 12 Sep 2016 09:53:03 -0600 Subject: [Histonet] Egg Whites and IHC Staining In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02BEEABA59F1@SBS2K8.premierlab.local> Jessica There are a couple of things that we will try here when we have problems with tissue adherence 1. We utilize a particular brand of plus slides - trubond 380 - these have worked very well in our hands, we purchase them from Newcomer Supply 2. Post fix in 10% NBF for 10 minutes after you run the slides down to water - this does not impact the H&E staining but can impact IHC staining we have seen that is may decrease sensitivity so you would need to potentially rework your IHC protocol 3. Our Ram-11 IHC protocol requires HIER we have two different retrieval protocols that we have validated for this particular antibody - our routine 95C for 30 minutes and then a 70C for 2 hours - we use the longer lower temp retrieval when we have problems with tissue adherence, again you may need to potentially rework your IHC protocol for the lower temp retrieval. Hope this helps, good luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Jessica Riggleman via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, September 12, 2016 8:05 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Egg Whites and IHC Staining Hello, We are cutting large Rabbit PLF formalin fixed paraffin embedded sections. We have had issues staining H&E and Goldner?s Trichrome. Egg whites is working as a great slide adhesive for these two, but not so much for IHC Staining (RAM 11 specifically). Any ideas/suggestions on how to get these sections to adhere? We have tried many methods, such as positively charged slides, chrom-alum gel, and so on. Thank you for all input, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brannon at alliedsearchpartners.com Mon Sep 12 12:41:01 2016 From: brannon at alliedsearchpartners.com (Brannon Owens) Date: Mon, 12 Sep 2016 17:41:01 +0000 Subject: [Histonet] Histotech job openings in Manitowoc, WI Message-ID: Histotechs needed for full time/permanent job opportunities in Manitowoc, WI. Relocation assistance and competitive pay offered - multiple shifts available. Send an update resume to brannon at alliedsearchpartners.com for review. To view a complete list of Allied Search Partners current openings go to: http://www.jobs.net/jobs/alliedsearchpartners/en-us/all-jobs/United-States/ Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 Direct Line: 407.413.9421 F: 888.388.7572 From jriggleman at globusmedical.com Mon Sep 12 12:44:37 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Mon, 12 Sep 2016 17:44:37 +0000 Subject: [Histonet] Egg Whites and IHC Staining In-Reply-To: <14E2C6176416974295479C64A11CB9AE02BEEABA59F1@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE02BEEABA59F1@SBS2K8.premierlab.local> Message-ID: Thank you Elizabeth. I will try those slides, I have not used them before. Do they have in 2x3? Is there a way you can send me your RAM11 protocol? Thank you, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. -----Original Message----- From: Elizabeth Chlipala [mailto:liz at premierlab.com] Sent: Monday, September 12, 2016 11:53 AM To: Jessica Riggleman ; 'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu) Subject: RE: Egg Whites and IHC Staining Jessica There are a couple of things that we will try here when we have problems with tissue adherence 1. We utilize a particular brand of plus slides - trubond 380 - these have worked very well in our hands, we purchase them from Newcomer Supply 2. Post fix in 10% NBF for 10 minutes after you run the slides down to water - this does not impact the H&E staining but can impact IHC staining we have seen that is may decrease sensitivity so you would need to potentially rework your IHC protocol 3. Our Ram-11 IHC protocol requires HIER we have two different retrieval protocols that we have validated for this particular antibody - our routine 95C for 30 minutes and then a 70C for 2 hours - we use the longer lower temp retrieval when we have problems with tissue adherence, again you may need to potentially rework your IHC protocol for the lower temp retrieval. Hope this helps, good luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Jessica Riggleman via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, September 12, 2016 8:05 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Egg Whites and IHC Staining Hello, We are cutting large Rabbit PLF formalin fixed paraffin embedded sections. We have had issues staining H&E and Goldner?s Trichrome. Egg whites is working as a great slide adhesive for these two, but not so much for IHC Staining (RAM 11 specifically). Any ideas/suggestions on how to get these sections to adhere? We have tried many methods, such as positively charged slides, chrom-alum gel, and so on. Thank you for all input, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Mon Sep 12 12:46:49 2016 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 12 Sep 2016 13:46:49 -0400 Subject: [Histonet] The NSH Convention is in Long Beach 9/16-9/21 Hope to See you there!! Message-ID: <059201d20d1d$a3f7bba0$ebe732e0$@earthlink.net> Hi Histonetters!! How are you? As you may know I do the majority of my work via email and phone so I rarely get a chance to meet people in person. That is one of the many reasons I enjoy attending the NSH conferences. I wanted to drop you a line and see if you or anyone you work with is planning on attending the NSH Conference in Long Beach next week? For more info here is the link: www.histoconvention.org I would love to have the opportunity to say Hi. I will be very easy to find because I am working the registration desk all day Friday and Saturday and Sunday morning AND The NSH membership booth in the exhibit hall on Sunday afternoon AND the Florida PR table on Monday So please stop by and say Hi! If you aren't attending this year follow the fun on FACEBOOK, INSTAGRAM and TWITTER. We will be posting with the hashtag #NSHSC We will be posting lots of pics and statuses about all the exciting stuff going on so stay tuned!! Also. If you are not able to attend are there any concerns or issues I can ask other techs about and report back to you? By the way. Here is a list of my current openings: HISTOLOGY SUPERVISORS/MANAGERS: Director of Laboratory Services - Stockton, CA Histology Lab Manager - Nashville, TN Lead Histology Tech - Las Cruces, NM HISTOTECHNICIANS/HISTOTECHNOLOGISTS: Histology Tech - Norfolk, VA - 15K sign on bonus plus relo. Histotech - Manitowoc, WI - learn new skills! NEW Client!! Histotech/IHC - Modesto, CA Histology Tech - Asheville, NC Histology Tech - Kingsport, TN All of these positions are full time and permanent and offer great salaries excellent benefits, relocation and/or sign on bonuses. For more information please contact me at relia1 at earthlink.net or on my cell at 407-353-5070 or toll free at 866-607-3542. Hope To See You in Long Beach!! Have a great day!! p.s. don't forget: let me know if there is anything I can find out for you there. Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From jriggleman at globusmedical.com Mon Sep 12 14:01:26 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Mon, 12 Sep 2016 19:01:26 +0000 Subject: [Histonet] TRAP Stain Message-ID: Can anyone recommend a TRAP (tartrate resistant acid phosphatase) protocol for formalin fixed paraffin embedded Rabbit PLF specimens? Or a kit to use? Thank you, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From jriggleman at globusmedical.com Mon Sep 12 14:16:46 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Mon, 12 Sep 2016 19:16:46 +0000 Subject: [Histonet] TRAP Stain Message-ID: Also if anyone has any photos of slides stained that would be great. We are looking for osteoclasts. _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. -----Original Message----- From: Jessica Riggleman Sent: Monday, September 12, 2016 3:01 PM To: 'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu) Subject: TRAP Stain Can anyone recommend a TRAP (tartrate resistant acid phosphatase) protocol for formalin fixed paraffin embedded Rabbit PLF specimens? Or a kit to use? Thank you, Jessica From lblazek at digestivespecialists.com Tue Sep 13 09:13:27 2016 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Tue, 13 Sep 2016 10:13:27 -0400 Subject: [Histonet] rotomat Message-ID: <5A2BD13465E061429D6455C8D6B40E391777EF9808@IBMB7Exchange.digestivespecialists.com> Good morning, Does anyone have any firsthand knowledge of the Hanel Rotomat slide storage system? Thanks, Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com From relia1 at earthlink.net Tue Sep 13 09:43:19 2016 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 13 Sep 2016 10:43:19 -0400 Subject: [Histonet] RELIA Hot Histology Job Alert Histotechs needed for rapidly growing Dermpath Lab in Manitowoc, WI Experienced techs and new grads needed!! Message-ID: <007c01d20dcd$2beb8dc0$83c2a940$@earthlink.net> Hi Histonetters! I hope you are all having a great day! If you are heading Long Beach for the NSH/SC I look forward to seeing you there! I also have a hot new histology opportunity to share: Histology Tech- Manitowoc, WI RELIA Solutions has been engaged by a rapidly growing dermpath lab located near in Manitowoc, WI to recruit an experienced certified histotech. You will be responsible for performing routine histology- cutting, embedding and staining. ASCP HT/HTL is required. histology experience is preferred and NEW GRADS WELCOME TO APPLY! This is a day shift position. My client offers excellent compensation, great benefits, relocation assistance and a great team to work with. My client is eager to speak with you and ready to hire!! For more information please contact Pam Barker at relia1 at earthlink.net or toll free at 866-607-3542. RELIA Solutions is the nation's ONLY recruiting firm specializing in the nationwide permanent placement of histology professionals. To sign up for our free histology careers bulletin please send an e-mail to relia1 at earthlink.net and include subscribe in the subject line. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From jcomensk at yahoo.com Tue Sep 13 12:55:24 2016 From: jcomensk at yahoo.com (John Comensky) Date: Tue, 13 Sep 2016 13:55:24 -0400 Subject: [Histonet] Old scope for donation? Message-ID: Histonetters- I'm looking for a basic microscope for use with some homeschooling children for science and chemistry class. If anybody knows of such a deal please email me. Many thanks. John Comensky, HT(ASCP)QIHC jcomensk at yahoo.com Sent from my iPhone From mills at 3scan.com Tue Sep 13 16:34:26 2016 From: mills at 3scan.com (Caroline Miller) Date: Tue, 13 Sep 2016 14:34:26 -0700 Subject: [Histonet] Old scope for donation? In-Reply-To: References: Message-ID: I am sure there are good options on this list, but I might also suggest AmScope. Not the most perfect of microscopes, but certainly reasonable and good enough for basic microscopy for kids. Make sure to chose an infinity corrected optic (i.e. go for a $200 one instead of the $30). They have staining kits too. Also the MSA website has a great resource on kids microscopy books: http://www.microscopy.org/education/projectmicro/searchBooks.cfm They have a booth as M&M, I really enjoyed looking at them all. I took photos of the ones I was interested in purchasing, I am happy to share these with you also. Let me know yours, mills On Tue, Sep 13, 2016 at 10:55 AM, John Comensky via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Histonetters- > > I'm looking for a basic microscope for use with some homeschooling > children for science and chemistry class. If anybody knows of such a deal > please email me. Many thanks. > > John Comensky, HT(ASCP)QIHC > jcomensk at yahoo.com > > Sent from my iPhone > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From vanessa.keeton03 at gmail.com Wed Sep 14 07:56:13 2016 From: vanessa.keeton03 at gmail.com (Vanessa Keeton) Date: Wed, 14 Sep 2016 08:56:13 -0400 Subject: [Histonet] HT Certification Exam Message-ID: Good morning all! I am getting ready to take my HT Certification Exam and was wondering if anyone would be willing to share some advice or thoughts on the exam which may help. Thanks in advance!! Vanessa Keeton From tgenade at gmail.com Wed Sep 14 10:02:32 2016 From: tgenade at gmail.com (Tyrone Genade) Date: Wed, 14 Sep 2016 10:02:32 -0500 Subject: [Histonet] head kidney issues Message-ID: Hello, I am doing IHC on fish tissues, in particular the head kidney. I am using DAB as chromogen. The tissue was fixed in Davidson's Fixative and processed for wax. Sections were dried in an oven overnight and then dewaxed and citrate was used for heat antigen retrieval. The sections were then permeablized with PBS-Triton and incubated in methanol with H202 for 30 min followed by 20 minutes in pH 2.3 citrate (biochemically speaking it should all be citric acid at this point) to denature any surviving endogenous peroxidase. The last step may seem a bit extreme but I was still getting signs of endogenous peroxidase activity in the head kidney sections after the methanol and still am. Now I wonder if it is something else? I found http://link.springer.com/article/10.1007/BF01003535 and saw this http://www.ncbi.nlm.nih.gov/pubmed/16242344 and now wonder if the issue isn't 5?-nucleotidase. The strong signal I am seeing has a granular appearance (macrophages?). Has anyone had similar experiences and found a solution? The former article implies using EDTA as an inhibitor. Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From naje1972 at yahoo.com Wed Sep 14 12:06:56 2016 From: naje1972 at yahoo.com (HistoQuenie) Date: Wed, 14 Sep 2016 12:06:56 -0500 Subject: [Histonet] head kidney issues In-Reply-To: References: Message-ID: <362516.79252.bm@smtp228.mail.bf1.yahoo.com> Good morning Mr. Genade Try using acetone/H202 in your blocking step. It may solve your problem with endogenous peroxide. Use the same amounts you would use for your blocking solution. I hope this helps. Cynthia James Sent from Mail for Windows 10 From: Tyrone Genade via Histonet From FMonson at wcupa.edu Wed Sep 14 13:15:24 2016 From: FMonson at wcupa.edu (Monson, Frederick) Date: Wed, 14 Sep 2016 18:15:24 +0000 Subject: [Histonet] Old news about cryo sections Message-ID: Back in the 1960's when I was learning all about the PAS method, I got my hands on a new CRYO-Stat (-30_!!!!!!!!!!!!!!!) and tried my 'stuff' on fresh frozen sections. Having been in the habit of controlling every variable I could name, I included a 'Schiff' control with each PAS. 1. Immediate reactions yielded negative control and positive PAS. It was late in the day, and I left the rest of my sections in the bottom of the cryostat and went home to the family. 2. Next morning reactions showed similar PAS and visible, but light, 'pink' in the control. 3. By 4:00pm I had very positive controls and similar PAS's. Since I had just finished getting A's in organic, I was ready for 'auto-aldehydes' via atmosphere, BUT my total funding for my thesis came to a little past $350.00, so I could not purchase or connive oxidation inhibitors or N2 to run thru the cryostat. 4. That experience, and things I kept hearing from all directions, led me to begin my histology and electron microscopy classes with biologists that "...everything you will 'see' this semester will be artifact!" 5. The last extension of that occurred when I had close to $900k of grant money, and I tested the dogma of permeability in an attempt to discover why 'God' would lay such a rich capillary bed under the epithelium of the urinary bladder of mammals when those epithelia were proved by "Ussing's Chamber" to be impermeable to almost everything! 6. My rule has been. "All experiments performed on dead or even freshly exposed tissue and cells are very likely artefactual." 7. Knowing that before I started, I still could only do what was possible, and almost all of that was not kind to the subject beyond anesthetics. 8. Yet; I am always aware of the truth of my rule, and that many times we perform experiments of color about which we are not as careful as we might be about the variables that we haven't attended to. 9. The most frightening experience I had when I was 'doing' science was when the last paper I wrote was accepted without comment. I have since determined that the explanation was the temporal irrelevance of the effort - i.e., it was almost 30 years too late to be of any real help. 10. I may at this moment be one of the very few US-university trained histologists, NOT a pathologist, that remains alive and working. Our $900k NIH grant may have been the last time they gave money to anatomist histologists. We did anatomy/histology and undergraduate physiology, and were published - except for the non-Ussing experiments. 11. OK here's the summary. 33 rabbits. Osmostic measurements on urine taken at catheterizing and that collected over the subsequent 1 hour. 1250 mosm +_ 50(?) for the urine in the bladder at the start. 350+_25(?) mosm after the hour collection that did not spend (much) time in the bladder. In human bladder mucosa (harvested from brain-dead subjects) I subsequently found aquaporins and a urea transporter. Then, time ran out, and the anatomists were unmasked. I must recommend to those of you who wonder where we are going with biology these days- and years ahead. Well, I strongly recommend that you search for a copy of the Hershey&Chase paper of 1952 and the "Structure of the T4 baseplate...." with movies taken from 3D-Tomographic and Structural Biological crystallographic data [ http://www.nature.com/nature/journal/v533/n7603/full/nature17971.html ] . One wonders if the T4 phage is always the same - what is its structural and 'functional range.' One more that I received from a non-scientist this morning: https://www.wired.com/2016/09/gorgeous-unsettling-video-evolution-action/#slide-1 Cheers to you all who labor among the rainbows of our tissues, Fred Monson who is being call 'dead wood' by few. I fear soon there will be many. Still, cheers!! -----Original Message----- From: HistoQuenie via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, September 14, 2016 1:07 PM To: Tyrone Genade via Histonet < > Subject: Re: [Histonet] head kidney issues Good morning Mr. Genade Try using acetone/H202 in your blocking step. It may solve your problem with endogenous peroxide. Use the same amounts you would use for your blocking solution. I hope this helps. Cynthia James Sent from Mail for Windows 10 From: Tyrone Genade via Histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren at gmail.com Wed Sep 14 13:32:04 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 14 Sep 2016 13:32:04 -0500 Subject: [Histonet] HT Certification Exam In-Reply-To: References: Message-ID: Get a good night's sleep and eat a good, high protein breakfast! There's always a question about which fixative to use or not use with uric acid crystals/gout, so I just gave you a free one! Good Luck! Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Sep 14, 2016 at 7:56 AM, Vanessa Keeton via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good morning all! > > I am getting ready to take my HT Certification Exam and was wondering if > anyone would be willing to share some advice or thoughts on the exam which > may help. > > Thanks in advance!! > > Vanessa Keeton > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From carl.hobbs at kcl.ac.uk Thu Sep 15 01:49:47 2016 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Thu, 15 Sep 2016 06:49:47 +0000 Subject: [Histonet] head kidney issues Message-ID: It's endogenous biotin, Tyrone. Make your own biotin blocking kit or buy RTU kit. Also, endog. peroxidases are eliminated by 3% aq H2O2 ( saves methanol) for 10mins at RT Also, don't bother with permeabilisation.....you've done all that by processing the tissue to Pwax Also, use 10% Formalin. That's what I use for my zfish Pwax IHC All suggestions: imho. Have a look in Histonet image archives for IHC Pwax sections of z.fish Best wishes Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From KSimeone at leavittmgt.com Thu Sep 15 06:59:38 2016 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Thu, 15 Sep 2016 11:59:38 +0000 Subject: [Histonet] FT OVERNIGHT HT POSTITION Delray Beach, FL In-Reply-To: <43944B1DBAAC2846B7B9D626B5F1233CC9194C6C@vm-email.leavittmgt.com> References: <43944B1DBAAC2846B7B9D626B5F1233CC9194C6C@vm-email.leavittmgt.com> Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CC919672A@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed HISTOTECHNOLOGIST here in our very busy Delray Beach Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Please fill out employment application HERE http://www.indeed.com/m/viewjob?jk=266fccd1fa688bb6&from=serp ^^you MUST follow this application link to apply! No exceptions. *FULL TIME position Mon-Fri OR Sun-Thurs 10p-6:30AM *MUST be licensed as a FL HISTOTECHNOLOGIST ONLY (will be working solo most of your shift) *MUST have at LEAST FIVE (5) years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE! *VERY proficient in embedding and microtomy *WILL MOSTLY BE EMBEDDING EXCISION BLOCKS, please know DERMS *must be self motivated, reliable and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) *some IHC experience preferred but not necessary Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor Delray Beach Technical Laboratory ADCS Clinics, LLC Advanced Dermatology and Cosmetic Surgery Ameriderm www.advancedderm.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From Linda.Margraf at cookchildrens.org Thu Sep 15 14:06:11 2016 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Thu, 15 Sep 2016 19:06:11 +0000 Subject: [Histonet] tissue lost in IHC Message-ID: <3f9d78307bec4660a1ae93598d4452e5@MBX10.CCHCS.LDAP> Here is a message I am posting for Wendy. Please note, respond to her or the list (not me directly). Also if you have problems posting messages, please check for embedded images, graphics etc. in your mail and try sending it plain text (which eliminates the graphics). The server often rejects messages with images or logos in the footers but won't send you a message letting you know there is a problem (this is something I cannot fix, sorry). Thanks, Linda Margraf Histonet administrator From: "Davis, Wendy" > Date: September 15, 2016 at 12:31:04 PM CDT To: "'histonet-owner at lists.utsouthwestern.edu'" > Subject: Tissue Loss Good Afternoon, I you would please post this to the Histonet list, I would greatly appreciate it. I am a member but the question has not posted in the past. The Pathologist that oversees our Immunohistochemistry laboratory has tasked me to find out from other institutions the percentage of repeats due to tissue loss every month. Does anyone keep statistics on this! I am most interested in finding statistics of loss when staining on the Ventana/Roche platform. Thank you all in advance for all of your helpful information. Wendy From garethdavisyuma at gmail.com Thu Sep 15 16:37:38 2016 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Thu, 15 Sep 2016 14:37:38 -0700 Subject: [Histonet] Low volume IHC instrument Message-ID: What IHC platform would you choose if you only stained for CD8 and H. pylori? Ventana, Leica Bond, or Biocare? Or is there another I am missing? Familiar with Dako, and don't think it would suit our lab. Thanks again. -- Ms. Gareth B. Davis, HT, QIHC (ASCPcm) Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From alauer2 at jhmi.edu Fri Sep 16 08:46:27 2016 From: alauer2 at jhmi.edu (Amanda Lauer) Date: Fri, 16 Sep 2016 13:46:27 +0000 Subject: [Histonet] removing araldite sections from glass slides Message-ID: <1474033587898.61454@jhmi.edu> Good morning, everyone. We have come into possession of a collection of mouse ears that were embedded in Araldite and intended for TEM. Most of the thick sections are mounted between sheets of Aclar. However, at some point there must have been a miscommunication with technician working with the tissue, and some of the specimens were mounted on glass slides and coverslipped. We believe Permount was used as the mounting medium. We would now like to re-embed some small pieces of the tissue for ultrathin sectioning. Is there a procedure for removing the cover slips and Araldite sections from the slides? I can't find anything online or in our old histology books. Thanks! ? Amanda Lauer, Ph.D. Assistant Professor Dept. of Otolaryngology-HNS Johns Hopkins University School of Medicine 443-287-4229 From edmartin26 at gmail.com Fri Sep 16 13:25:26 2016 From: edmartin26 at gmail.com (Eddie Martin) Date: Fri, 16 Sep 2016 14:25:26 -0400 Subject: [Histonet] Low volume IHC instrument In-Reply-To: References: Message-ID: <4EF76559-054D-4717-AE32-F2FE3372417E@gmail.com> Hi Gareth, If Fako doesn't suit your needs, then bio are wouldn't either. The closest analyzer with full onboard retrieval that is also more open without purchasing separate detection kits to improve quality is the Leica Bond. H pylori retrieval on Bond is only 10 mins and I think CD8 is 20 mins I think. So the overall stain time would be a few hours on the Leica Bond-max and about an hour less on the Bond-III. It shaves off at least 45 mins from the time spent by a bond-max platform. I have also used Ventana XT, and ultra. We can discuss further if you would like to call me at 954-826-9403. Hope this helps, Eddie Martin HT(ASCP),QIHC, HTL Sent from my iPhone > On Sep 15, 2016, at 5:37 PM, Gareth Davis wrote: > > What IHC platform would you choose if you only stained for CD8 and H. > pylori? > Ventana, Leica Bond, or Biocare? Or is there another I am missing? > Familiar with Dako, and don't think it would suit our lab. > Thanks again. > > > -- > Ms. Gareth B. Davis, HT, QIHC (ASCPcm) > Yuma Gastroenterology > Yuma, AZ 85364 > 928-248-5259 > From blayjorge at gmail.com Fri Sep 16 14:29:50 2016 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Fri, 16 Sep 2016 15:29:50 -0400 Subject: [Histonet] Looking for commercial source of casein (5-10g) Message-ID: Dear Histonetters: I need 5-10 grams of casein for a protocol I am testing. The two recommended sources only supply casein at the kg level, much more than what I need. Can someone (off the list, please) suggest me a place that may sell 5-10 grams? If you have any constructive suggestions, please email me directly at blayjorge at gmail.com Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From blayjorge at gmail.com Fri Sep 16 14:43:02 2016 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Fri, 16 Sep 2016 15:43:02 -0400 Subject: [Histonet] Change in linear dimensions of soft tissues in larval insect when fixed Message-ID: Change in linear dimensions of soft tissues in larval insect when fixed Dear Histonetters: I have nearly ca. 200 museum specimens of aquatic larval insects (1-3 cm long) that I assume were killed by dumping them in (at the minimum) 70% ethanol. In the only one case that the label states anything about preservation method, it reads "KAAD --> 95%". I assume that several changes in ethanol 70% have taken place to refill vials, as needed, in the 48-77 years since the specimens have been dead. Question: While the hard body parts will barely change in dimension with time, does anyone know how does the softer body parts change in size? Is there any variation in size change whether the preservation took place early or late in the instar? If you have any constructive suggestions, please email me directly at blayjorge at gmail.com Apologies for potential duplicate emails. Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From SteveM at mcclainlab.com Sat Sep 17 08:15:58 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Sat, 17 Sep 2016 13:15:58 +0000 Subject: [Histonet] Histonet Digest, Vol 154, Issue 13 tissue loss In-Reply-To: References: Message-ID: <0FA48E92-0CF1-41DC-8C69-4DC511CB6E9D@mcclainlab.com> Wendy Develop your own data. Check your early steps first. Tissue loss can be simple -a function of poor fixation, dirty processing or rush processing or slightly large tissue w slightly dirty reagents. Airdry wet slides before annealing on hot plate. Check your baking/annealing. Some tissues require a bit more. We do not use that stainer. Check your heat antigen retrieval against a manual retrieval. Skin can handle 90 degrees for twice as long as 98. 98 degrees may cause excessive collagen loss. Steve A. McClain, MD > On Sep 16, 2016, at 13:25, "histonet-request at lists.utsouthwestern.edu" wrote: > > Wendy From tbraud at holyredeemer.com Mon Sep 19 07:51:27 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 19 Sep 2016 12:51:27 +0000 Subject: [Histonet] Low Volume IHC Message-ID: <48E053DDF6CE074DB6A7414BA05403F808C426@HRHEX02-HOS.holyredeemer.local> Hi Gareth! BioCare has a wonderful instrument, the Oncor, that has a 30 slide max, with full onboard antigen retrieval. Super simple to operate. We were a Beta site to test and all the techs really liked it. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Today's Topics: 1. Re: Low volume IHC instrument (Eddie Martin) From lmarie08 at uga.edu Mon Sep 19 12:32:18 2016 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Mon, 19 Sep 2016 17:32:18 +0000 Subject: [Histonet] taking the HTL Message-ID: Hello all, I was wondering if anyone out there has used the HTL practice tests package you can buy from ASCP to prepare for their exam, and if you did was it worth $30? Thanks! From JMacDonald at mtsac.edu Mon Sep 19 15:22:40 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Mon, 19 Sep 2016 13:22:40 -0700 Subject: [Histonet] taking the HTL In-Reply-To: References: Message-ID: This may be a better option: https://www.labce.com/histology_exam_simulator.aspx The NSH has partnered with media lab for exams. You have a full year access to the questions. There are over 2,000 questions in the question bank and there are images as well. NSH members pay the discounted rate of $79. Non NSH members pay $99. Jennifer From: Lauren Sweeney via Histonet To: "histonet at lists.utsouthwestern.edu" Date: 09/19/2016 10:36 AM Subject: [Histonet] taking the HTL Hello all, I was wondering if anyone out there has used the HTL practice tests package you can buy from ASCP to prepare for their exam, and if you did was it worth $30? Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbmphoto at gmail.com Mon Sep 19 17:26:08 2016 From: mbmphoto at gmail.com (Maria Mejia) Date: Mon, 19 Sep 2016 15:26:08 -0700 Subject: [Histonet] slide labeler advice for research lab Message-ID: <65A9B277-3A39-4C63-BE15-EE59BFD3B10D@gmail.com> Our research lab is in the market for a slide labeler. We are an IHC research lab (NOT clinical) working on both paraffin & thick free-floating human whole brain & brainstem sections. Since the lab has never used a slide labeler before (we?ve been manually making our labels), the labeler should be easy to use, so no one has to spend a lot of time figuring & programming the unit. Since we use paraffin standard glass slides as well as 2 inche x 3 inch glass slides, we need a labeler that we could create formats for different sample sizes and types e.g. ID #, Casp 6 + TH IHC + (name of counterstain used) & date..etc. The unit should also have barcoding capabilities. Any advice or suggestions are appreciated. Vendors welcome also. Maria Mejia Lead Histologist Memory & Aging Center UCSF SF CA From esarricks at gmail.com Tue Sep 20 11:11:46 2016 From: esarricks at gmail.com (Erin Sarricks) Date: Tue, 20 Sep 2016 12:11:46 -0400 Subject: [Histonet] Searching for Horizontal Slide Racks Message-ID: Hi Histonet- I sent out this post about a year ago and had a great response, but we are in need of additional slide racks now. Does anyone have any of these racks that hold slides horizontally that they no longer need? Like this but for maybe 50-60 slides? http://grale.com.au/products/view/297 I am willing to purchase and pay for shipping if anyone has any they are getting rid of. Thank you! Erin From ADuddey at firsthealth.org Wed Sep 21 07:01:04 2016 From: ADuddey at firsthealth.org (Duddey, Aimee) Date: Wed, 21 Sep 2016 12:01:04 +0000 Subject: [Histonet] Assigning cases to pathologists in EPIC Message-ID: <09FBA01CA9B6374A83C5C76E09E46188A34ABE1E@EXMAIL1-FHC.firsthealth.org> For anyone currently using EPIC Beaker or any other system where you assign pathologists at accession, please share how you assign cases. We are still currently using transcriptionists who assign the case to a pathologist as they listen to them dictate. For purposes of outstanding lists we would like to assign cases as soon in the process as possible. Please share how you doings this. Thank you in advance, Aimee M. Duddey, MLT(ASCP) Assistant Director of Laboratory - Pathology FH Moore Regional Hospital 910-715-5286 office 910715-1944 fax From lmarie08 at uga.edu Wed Sep 21 08:17:22 2016 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Wed, 21 Sep 2016 13:17:22 +0000 Subject: [Histonet] veterinary histology Message-ID: To all you veterinary histotechs out there: We got a case submission of a whole 44 week old chicken head and the write up states "swollen head and dirty ear canals". This is the first time we have seen this, even the pathologist is stumped. What part(s) of the head would you gross, and how? Any feedback welcome! Thanks! From jriggleman at globusmedical.com Wed Sep 21 09:58:49 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Wed, 21 Sep 2016 14:58:49 +0000 Subject: [Histonet] Vortex Mixer - Sale? Message-ID: Hello, Does anyone have an old vortex mixer they do not need, such as the one below: http://www.ebay.com/itm/Fisher-G-560-Vortex-Genie-2-Lab-Mixer-Disc-Platform-Shaker-12-812-Ref-A-/282149263394?hash=item41b1681822:g:IwIAAOSwZVlXvu9V I am willing to pay you and willing to pay shipping. Thank you, Jessica Riggleman _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From j.benavides at eae.csic.es Wed Sep 21 11:56:41 2016 From: j.benavides at eae.csic.es (Julio Benavides) Date: Wed, 21 Sep 2016 18:56:41 +0200 Subject: [Histonet] veterinary histology In-Reply-To: References: Message-ID: <20160921185641.Horde.RelnV5lIO9lVPuDvWbu28Q8@webmail.csic.es> Hi there, could be E. coli+virus ("swallen head syndrome") or fowl cholera (P. multocida). I would check the nasal cavity (slice the peak longitudinally) and also the infraorbital sinuses. I am at aloss regarding the ear discharge. Try a coronal slice of the cranium at the ear opening. A sharp knife should be enough to do the cutting. If not, try to decalcified the whole head for a couple of days. Then, decal (if you have not done it previously) the cut tissue before processing. By the way, if you have any fresh (non fixed) head, to take a sample from the exudate and do some microbiology could be tremendously helpful in these cases. Hope this helps Cheers Julio Lauren Sweeney via Histonet escribi?: > To all you veterinary histotechs out there: > > We got a case submission of a whole 44 week old chicken head and the > write up states "swollen head and dirty ear canals". This is the > first time we have seen this, even the pathologist is stumped. What > part(s) of the head would you gross, and how? > > Any feedback welcome! > > Thanks! > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jriggleman at globusmedical.com Wed Sep 21 12:05:30 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Wed, 21 Sep 2016 17:05:30 +0000 Subject: [Histonet] Positive Control for TRAP Staining Message-ID: Hello, Does anyone have a suggestion for a good positive control for TRAP staining? Thank you, Jessica Riggleman _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From abadesuyi at nrh-ok.com Wed Sep 21 12:54:06 2016 From: abadesuyi at nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Wed, 21 Sep 2016 12:54:06 -0500 Subject: [Histonet] CAP Inspection Message-ID: <04EE4F75BB5FB246ADB68D69B7460443B1006B613B@MAIL.nrhnt.nrh-ok.com> Hi, We had our CAP Inspection last week. I had two Pathologists and a Cytotechnologist (we don't do cytology) with me all day. There was no Histotechnologist on the team. I am just curious, whether any of you have had this type of inspection before. Best regards, Banjo Adesuyi ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From mills at 3scan.com Wed Sep 21 14:38:00 2016 From: mills at 3scan.com (Caroline Miller) Date: Wed, 21 Sep 2016 12:38:00 -0700 Subject: [Histonet] Unprocessing tissue from paraffin Message-ID: Hi there Histonetters! I am currently working through some issues of unprocessing tissue to then restain 'whole mount' with various stains, and then reprocess back to paraffin. I am running into issues of tissue being brittle and scratchy tissue after reprocessing and sectioning. Can anyone offer any advice on kinder dewaxing procedures and / or additives to the unprocessing / processing fluids that might help in the resulting piece of tissue? I have currently been putting it through the cleaning cycle on the processor, so standard xylene then alcohol treatment. thanks in advance for your advice! mills -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From Blanca.Lopez at UTSouthwestern.edu Wed Sep 21 14:59:17 2016 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Wed, 21 Sep 2016 19:59:17 +0000 Subject: [Histonet] Mousse tissue processing Message-ID: <0d9b69779c50481c8c1be992255a880c@SWMS13MAIL12.swmed.org> Hello! Histonets. Does anyone has a good protocol for processing mouse tissue? Somebody can share. thanks Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From anrecord at saintfrancis.com Wed Sep 21 15:06:07 2016 From: anrecord at saintfrancis.com (Record, Angela N) Date: Wed, 21 Sep 2016 20:06:07 +0000 Subject: [Histonet] Quotes for capital Message-ID: <20FC170D97CF6546939ABB0FA907C8DB039C70@SFHSEXCH001.WarrenNT.SaintFrancis.Loc> Greg, Could you please send me 2 separate budgetary quotes for a VIP6 and a Prisma? Thank You, Angela Record, BS, HT(ASCP) Saint Francis Hospital Tulsa, OK 918-494-1321 ---------------------------------------------------------------------- Saint Francis Health System intends this email only for the use of the person to whom it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you have received this email in error, you are hereby notified that we do not consent to any reading, dissemination, distribution, or copying of this email and request you notify the sender immediately and destroy this transmission. Violators may be prosecuted under Federal law. From mills at 3scan.com Wed Sep 21 17:37:03 2016 From: mills at 3scan.com (Caroline Miller) Date: Wed, 21 Sep 2016 15:37:03 -0700 Subject: [Histonet] Mousse tissue processing In-Reply-To: <0d9b69779c50481c8c1be992255a880c@SWMS13MAIL12.swmed.org> References: <0d9b69779c50481c8c1be992255a880c@SWMS13MAIL12.swmed.org> Message-ID: Hi Blanca, This is what I use, note, no formalin on my machine, and you could probably cut out one of the last alcohols Step Routine tissue 70% alcohol 10 80% alcohol 10 90% alcohol 20 100% alcohol 20 100% alcohol 20 100% alcohol 30 100% alcohol 30 100% alcohol 30 Xylene 30 Xylene 35 paraffin 30 paraffin 30 paraffin 30 paraffin 30 paraffin at 60oC, P/V on, agit on On Wed, Sep 21, 2016 at 12:59 PM, Blanca Lopez via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello! Histonets. > Does anyone has a good protocol for processing mouse tissue? Somebody can > share. > thanks > > Blanca Lopez > Histotech (ASCP) > UTSW Tissue Resource K1.210 > Simmons Comprehensive Cancer Center > UT Southwestern Medical Center > Telephone: 214-648-7598 > Email: Blanca.Lopez at utsouthwestern.edu > > > ________________________________ > > UT Southwestern > > > Medical Center > > > > The future of medicine, today. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From mbmphoto at gmail.com Wed Sep 21 21:10:06 2016 From: mbmphoto at gmail.com (Maria Mejia) Date: Wed, 21 Sep 2016 19:10:06 -0700 Subject: [Histonet] Unprocessing tissue from paraffin In-Reply-To: References: Message-ID: Hi Millis, Lets see if I?m understanding you correctly. 1) You have unprocessed tissue. What kind of unprocessed tissue & how thick are the free-floating sections? 2) Are the tissue fixed? If so, with what? & For how long? 3) You want to stain the unknown whole mount using various histochemical stains or IHC stains? 4) Can you please explain why after staining you need to reprocess for paraffin? Cause once these mystery sections are processed you'll have to cut them using a rotary microtome. I can maybe help you, but your going to have to provide more detail information. Also provide your processing protocol. Regards Maria Mejia Lead Histologist Memory & Aging Center UCSF > On Sep 21, 2016, at 12:38 PM, Caroline Miller via Histonet wrote: > > Hi there Histonetters! > > I am currently working through some issues of unprocessing tissue to then > restain 'whole mount' with various stains, and then reprocess back to > paraffin. > > I am running into issues of tissue being brittle and scratchy tissue after > reprocessing and sectioning. > > Can anyone offer any advice on kinder dewaxing procedures and / or > additives to the unprocessing / processing fluids that might help in the > resulting piece of tissue? > > I have currently been putting it through the cleaning cycle on the > processor, so standard xylene then alcohol treatment. > > thanks in advance for your advice! > mills > > > > > -- > Caroline Miller (mills) > Director of Histology > 3Scan.com > 415 2187297 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Thu Sep 22 12:25:45 2016 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 22 Sep 2016 13:25:45 -0400 Subject: [Histonet] RELIA Hot Histology Job Alert. Dermpath histology opportunities available nationwide for experienced techs and new grads!! Message-ID: <013d01d214f6$5ac81ca0$105855e0$@earthlink.net> Hi Histonetters!! I hope everyone is having a great day. I am posting the info on some exciting opportunities I am working on. If you or anyone you know is interested please contact me. Here is the info: RELIA Solutions specializes in the nationwide permanent placement of histology professionals. We have been engaged by several leading dermatopathology labs nationwide to recruit top notch histotechs for their facilities. Our clients offer an excellent salary, a great benefits, relocation and or sign on bonuses and great groups of people to work with. They are eager to interview and hire. ASCP certification, HT/HTL is required. My clients are in need of experienced and entry level techs. The positions are located in the following areas: Wisconsin California Arkansas Texas For more information please contact Pam Barker at relia1 at earthlink.net or toll free at 866-607-3542. To sign up for our free histology careers bulletin please send an e-mail to relia1 at earthlink.net and include subscribe in the subject line. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From bhartologist at gmail.com Thu Sep 22 13:02:18 2016 From: bhartologist at gmail.com (Bharti Parihar) Date: Thu, 22 Sep 2016 11:02:18 -0700 Subject: [Histonet] Histonet Digest, Vol 154, Issue 18 In-Reply-To: References: Message-ID: Hello all! If any licensed Histotechs are interested in working in the San Francisco Bay area, Kaiser Permanente in Berkeley is hiring! Please send me an email so I can assist on getting you on board! On Sep 22, 2016 10:17 AM, wrote: Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Positive Control for TRAP Staining (Jessica Riggleman) 2. CAP Inspection (Adesupo, Adesuyi (Banjo)) 3. Unprocessing tissue from paraffin (Caroline Miller) 4. Mousse tissue processing (Blanca Lopez) 5. Quotes for capital (Record, Angela N) 6. Re: Mousse tissue processing (Caroline Miller) 7. Re: Unprocessing tissue from paraffin (Maria Mejia) ---------------------------------------------------------------------- Message: 1 Date: Wed, 21 Sep 2016 17:05:30 +0000 From: Jessica Riggleman To: "'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)" Subject: [Histonet] Positive Control for TRAP Staining Message-ID: Content-Type: text/plain; charset="utf-8" Hello, Does anyone have a suggestion for a good positive control for TRAP staining? Thank you, Jessica Riggleman _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. ------------------------------ Message: 2 Date: Wed, 21 Sep 2016 12:54:06 -0500 From: "Adesupo, Adesuyi (Banjo)" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] CAP Inspection Message-ID: <04EE4F75BB5FB246ADB68D69B7460443B1006B613B at MAIL.nrhnt.nrh-ok.com> Content-Type: text/plain; charset="us-ascii" Hi, We had our CAP Inspection last week. I had two Pathologists and a Cytotechnologist (we don't do cytology) with me all day. There was no Histotechnologist on the team. I am just curious, whether any of you have had this type of inspection before. Best regards, Banjo Adesuyi ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ Message: 3 Date: Wed, 21 Sep 2016 12:38:00 -0700 From: Caroline Miller To: "Histonet at Lists. Edu" Subject: [Histonet] Unprocessing tissue from paraffin Message-ID: Content-Type: text/plain; charset=UTF-8 Hi there Histonetters! I am currently working through some issues of unprocessing tissue to then restain 'whole mount' with various stains, and then reprocess back to paraffin. I am running into issues of tissue being brittle and scratchy tissue after reprocessing and sectioning. Can anyone offer any advice on kinder dewaxing procedures and / or additives to the unprocessing / processing fluids that might help in the resulting piece of tissue? I have currently been putting it through the cleaning cycle on the processor, so standard xylene then alcohol treatment. thanks in advance for your advice! mills -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 4 Date: Wed, 21 Sep 2016 19:59:17 +0000 From: Blanca Lopez To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Mousse tissue processing Message-ID: <0d9b69779c50481c8c1be992255a880c at SWMS13MAIL12.swmed.org> Content-Type: text/plain; charset="us-ascii" Hello! Histonets. Does anyone has a good protocol for processing mouse tissue? Somebody can share. thanks Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. ------------------------------ Message: 5 Date: Wed, 21 Sep 2016 20:06:07 +0000 From: "Record, Angela N" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Quotes for capital Message-ID: <20FC170D97CF6546939ABB0FA907C8DB039C70 at SFHSEXCH001.WarrenNT. SaintFrancis.Loc> Content-Type: text/plain; charset="us-ascii" Greg, Could you please send me 2 separate budgetary quotes for a VIP6 and a Prisma? Thank You, Angela Record, BS, HT(ASCP) Saint Francis Hospital Tulsa, OK 918-494-1321 ---------------------------------------------------------------------- Saint Francis Health System intends this email only for the use of the person to whom it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you have received this email in error, you are hereby notified that we do not consent to any reading, dissemination, distribution, or copying of this email and request you notify the sender immediately and destroy this transmission. Violators may be prosecuted under Federal law. ------------------------------ Message: 6 Date: Wed, 21 Sep 2016 15:37:03 -0700 From: Caroline Miller To: Blanca Lopez Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Mousse tissue processing Message-ID: Content-Type: text/plain; charset=UTF-8 Hi Blanca, This is what I use, note, no formalin on my machine, and you could probably cut out one of the last alcohols Step Routine tissue 70% alcohol 10 80% alcohol 10 90% alcohol 20 100% alcohol 20 100% alcohol 20 100% alcohol 30 100% alcohol 30 100% alcohol 30 Xylene 30 Xylene 35 paraffin 30 paraffin 30 paraffin 30 paraffin 30 paraffin at 60oC, P/V on, agit on On Wed, Sep 21, 2016 at 12:59 PM, Blanca Lopez via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello! Histonets. > Does anyone has a good protocol for processing mouse tissue? Somebody can > share. > thanks > > Blanca Lopez > Histotech (ASCP) > UTSW Tissue Resource K1.210 > Simmons Comprehensive Cancer Center > UT Southwestern Medical Center > Telephone: 214-648-7598 > Email: Blanca.Lopez at utsouthwestern.edu > > > ________________________________ > > UT Southwestern > > > Medical Center > > > > The future of medicine, today. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 7 Date: Wed, 21 Sep 2016 19:10:06 -0700 From: Maria Mejia To: Caroline Miller Cc: "Histonet at Lists. Edu" Subject: Re: [Histonet] Unprocessing tissue from paraffin Message-ID: Content-Type: text/plain; charset=utf-8 Hi Millis, Lets see if I?m understanding you correctly. 1) You have unprocessed tissue. What kind of unprocessed tissue & how thick are the free-floating sections? 2) Are the tissue fixed? If so, with what? & For how long? 3) You want to stain the unknown whole mount using various histochemical stains or IHC stains? 4) Can you please explain why after staining you need to reprocess for paraffin? Cause once these mystery sections are processed you'll have to cut them using a rotary microtome. I can maybe help you, but your going to have to provide more detail information. Also provide your processing protocol. Regards Maria Mejia Lead Histologist Memory & Aging Center UCSF > On Sep 21, 2016, at 12:38 PM, Caroline Miller via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > Hi there Histonetters! > > I am currently working through some issues of unprocessing tissue to then > restain 'whole mount' with various stains, and then reprocess back to > paraffin. > > I am running into issues of tissue being brittle and scratchy tissue after > reprocessing and sectioning. > > Can anyone offer any advice on kinder dewaxing procedures and / or > additives to the unprocessing / processing fluids that might help in the > resulting piece of tissue? > > I have currently been putting it through the cleaning cycle on the > processor, so standard xylene then alcohol treatment. > > thanks in advance for your advice! > mills > > > > > -- > Caroline Miller (mills) > Director of Histology > 3Scan.com > 415 2187297 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 154, Issue 18 ***************************************** From alicia.ortega at colorado.edu Thu Sep 22 15:50:28 2016 From: alicia.ortega at colorado.edu (Alicia Marie Ortega) Date: Thu, 22 Sep 2016 20:50:28 +0000 Subject: [Histonet] Question on Sanderson Rapid Bone Stain Message-ID: Hello everyone, I am attempting to stain 30-40 micron thick undecalcified bone sections embedded in poly(methyl methacrylate) with Sanderson Rapid Bone Stain (RBS) with a Van Geison counter stain. My first attempt at this stain resulted in a very faint stain from the RBS (I could see very faint blue/green staining of osteoid/soft tissues) and a very intense dark pink stain from the counterstain. I was wondering if anyone knows of a way to intensify the staining of the RBS? I heated the stain to 55-60 degrees Celsius as recommended by the manufacturer prior to staining (with a 10 minute stain duration). Thank you in advance for your time and help. Sincerely, Alicia Ortega Postdoctoral Research Associate University of Colorado, Boulder From Sheri.Meilus at va.gov Fri Sep 23 05:21:30 2016 From: Sheri.Meilus at va.gov (Meilus, Sheri D.) Date: Fri, 23 Sep 2016 06:21:30 -0400 Subject: [Histonet] Adipose Tissue on Frozen Sections Message-ID: Hello Netters, Does anyone have a protocol for optimizing section quality for specimens with considerable adipose tissue to produce cryostat sections? We have had several instances recently with derm specimens with considerable subcutaneous adipose tissue as well as lymph nodes with surrounding pericapsular fat and surgeons demanding margins. We perform frozen sections on standard Leica cryostats at -20 +/- 2 degrees. Anyone out there use liquid nitrogen or another piece of equipment that would freeze the specimen at a lower temperature than a standard cryostat? Thanks! Sheri S Sheri Meilus HT (ASCP) QIHC Building 100, Room 2B-109 727-398-6661 ext 14596/14146 From lmarie08 at uga.edu Fri Sep 23 07:10:00 2016 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Fri, 23 Sep 2016 12:10:00 +0000 Subject: [Histonet] siphon drum pump Message-ID: Good morning and happy Friday everyone! I am looking to buy some siphon drum pumps- does anyone out there use them? We have three and they are all leaking at the squeeze/bulb area from the xylene breaking it down. Does anyone know if they manufacture some that are coated to protect against xylene? Thanks! From sandra.cheasty at wisc.edu Fri Sep 23 09:41:17 2016 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Fri, 23 Sep 2016 14:41:17 +0000 Subject: [Histonet] Microm HM 355S Issues Message-ID: Hello, We have 2 Microm HM355S motorized microtomes. We use the motor to move the block close to the blade, but turn the wheel manually when sectioning. Both are "jumping" forward on their own occasionally while sectioning, which is unsettling and chunks into the block. One of them also backs up on its own as you're manually turning the handle, just as you're about to get a ribbon. The stepper motor has been replaced on one with no improvement. Any suggestions, remedies, or tales of similar issues and how they were resolved would be most appreciated. Thanks, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From ADuddey at firsthealth.org Fri Sep 23 10:29:46 2016 From: ADuddey at firsthealth.org (Duddey, Aimee) Date: Fri, 23 Sep 2016 15:29:46 +0000 Subject: [Histonet] Microm HM 355S Issues In-Reply-To: References: Message-ID: <09FBA01CA9B6374A83C5C76E09E46188A34ADB3A@EXMAIL1-FHC.firsthealth.org> We are currently having the same problem with 2 of ours. Stepper motor replacement did not work either. I would be interested to hear any responses as well. Aimee -----Original Message----- From: Sandra Cheasty via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, September 23, 2016 10:41 AM To: Histonet (histonet at lists.utsouthwestern.edu) Cc: BARBARA J REESE Subject: [Histonet] Microm HM 355S Issues Hello, We have 2 Microm HM355S motorized microtomes. We use the motor to move the block close to the blade, but turn the wheel manually when sectioning. Both are "jumping" forward on their own occasionally while sectioning, which is unsettling and chunks into the block. One of them also backs up on its own as you're manually turning the handle, just as you're about to get a ribbon. The stepper motor has been replaced on one with no improvement. Any suggestions, remedies, or tales of similar issues and how they were resolved would be most appreciated. Thanks, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren at gmail.com Fri Sep 23 12:16:14 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 23 Sep 2016 12:16:14 -0500 Subject: [Histonet] Microm HM 355S Issues In-Reply-To: <09FBA01CA9B6374A83C5C76E09E46188A34ADB3A@EXMAIL1-FHC.firsthealth.org> References: <09FBA01CA9B6374A83C5C76E09E46188A34ADB3A@EXMAIL1-FHC.firsthealth.org> Message-ID: With most microtomes, 1 complete turn of the wheel equals one advance of four microns, or whatever you have the micrometer set on. What people don't realize with the HM355 is that is doesn't take a complete turn of the fine advance wheel to advance the specimen. If I recall correctly, the advance will happen when the wheel rotates somewhere between 90 and 180 degrees, or when the handle is from the 9 to 6 'o'clock position (the locking position being 12 o'clock.) People don't realize it has advanced, because they haven't made a full turn of the wheel as they are rough cutting, then when they start their fine cutting, chunkola! With the HM355 microtomes, it is unwise to rough cut by "rocking". If you bring the wheel past a certain point, the sensor reads it as a complete turn of the wheel and advances the block. If you do it once, you might only get a thick section, but if it happens 2 or 3 times in the course of rough cutting, you will chunk out your block. It is safer, although much slower, to rough cut by setting the micrometer on 10-15 microns and rotating the fine advance wheel normally. Or, if you are very patient and careful, you can learn the specific spot your microtome advances at, and rough cut by rocking, but just being careful never to rock the wheel past the position where it will advance the specimen. Sincerely, Jay A. Lundgren, MS On Fri, Sep 23, 2016 at 10:29 AM, Duddey, Aimee via Histonet < histonet at lists.utsouthwestern.edu> wrote: > We are currently having the same problem with 2 of ours. Stepper motor > replacement did not work either. I would be interested to hear any > responses as well. > > Aimee > > > -----Original Message----- > From: Sandra Cheasty via Histonet [mailto:histonet at lists. > utsouthwestern.edu] > Sent: Friday, September 23, 2016 10:41 AM > To: Histonet (histonet at lists.utsouthwestern.edu) > Cc: BARBARA J REESE > Subject: [Histonet] Microm HM 355S Issues > > Hello, > We have 2 Microm HM355S motorized microtomes. We use the > motor to move the block close to the blade, but turn the wheel manually > when sectioning. Both are "jumping" forward on their own occasionally while > sectioning, which is unsettling and chunks into the block. One of them also > backs up on its own as you're manually turning the handle, just as you're > about to get a ribbon. > The stepper motor has been replaced on one with no > improvement. Any suggestions, remedies, or tales of similar issues and how > they were resolved would be most appreciated. > Thanks, > Sandy > > Sandra J. Cheasty, HT (ASCP) > Histology & Necropsy Supervisor > UW-Madison, School of Veterinary Medicine > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jriggleman at globusmedical.com Fri Sep 23 12:24:38 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Fri, 23 Sep 2016 17:24:38 +0000 Subject: [Histonet] siphon drum pump In-Reply-To: References: Message-ID: Lauren, What is your pump made out of? _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. -----Original Message----- From: Lauren Sweeney [mailto:lmarie08 at uga.edu] Sent: Friday, September 23, 2016 8:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] siphon drum pump Good morning and happy Friday everyone! I am looking to buy some siphon drum pumps- does anyone out there use them? We have three and they are all leaking at the squeeze/bulb area from the xylene breaking it down. Does anyone know if they manufacture some that are coated to protect against xylene? Thanks! From jriggleman at globusmedical.com Fri Sep 23 12:42:09 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Fri, 23 Sep 2016 17:42:09 +0000 Subject: [Histonet] Question on Sanderson Rapid Bone Stain In-Reply-To: References: Message-ID: RBS is the same as Methylene Blue, I believe. _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. -----Original Message----- From: Alicia Marie Ortega [mailto:alicia.ortega at colorado.edu] Sent: Thursday, September 22, 2016 4:50 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Question on Sanderson Rapid Bone Stain Hello everyone, I am attempting to stain 30-40 micron thick undecalcified bone sections embedded in poly(methyl methacrylate) with Sanderson Rapid Bone Stain (RBS) with a Van Geison counter stain. My first attempt at this stain resulted in a very faint stain from the RBS (I could see very faint blue/green staining of osteoid/soft tissues) and a very intense dark pink stain from the counterstain. I was wondering if anyone knows of a way to intensify the staining of the RBS? I heated the stain to 55-60 degrees Celsius as recommended by the manufacturer prior to staining (with a 10 minute stain duration). Thank you in advance for your time and help. Sincerely, Alicia Ortega Postdoctoral Research Associate University of Colorado, Boulder From thigginsht at msn.com Fri Sep 23 13:01:04 2016 From: thigginsht at msn.com (T H) Date: Fri, 23 Sep 2016 18:01:04 +0000 Subject: [Histonet] Subject: CAP Inspection In-Reply-To: References: Message-ID: Hey Banjo, Yes, I have been inspected without any real representative from histology. My current employer sends a Pathologist, PhD and the AP Manager (Cytotechnologist with no real Histology experience) to our CAP inspection. The person really doesn't need to know anything about Histology to do a inspection, just be able to follow the CAP checklist questions and have the facility being inspected provide proof of compliance. Anything specific to Histology and they would not know if someone is pulling their leg or not. TH ------------------------------ Message: 2 Date: Wed, 21 Sep 2016 12:54:06 -0500 From: "Adesupo, Adesuyi (Banjo)" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] CAP Inspection Message-ID: <04EE4F75BB5FB246ADB68D69B7460443B1006B613B at MAIL.nrhnt.nrh-ok.com> Content-Type: text/plain; charset="us-ascii" Hi, We had our CAP Inspection last week. I had two Pathologists and a Cytotechnologist (we don't do cytology) with me all day. There was no Histotechnologist on the team. I am just curious, whether any of you have had this type of inspection before. Best regards, Banjo Adesuyi ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From PKRomund at gundersenhealth.org Fri Sep 23 13:32:23 2016 From: PKRomund at gundersenhealth.org (Romundstad, Pamela K) Date: Fri, 23 Sep 2016 18:32:23 +0000 Subject: [Histonet] FW: Coplin Jar cleaning In-Reply-To: References: Message-ID: Hi, Does anyone have a chirp proof way of cleaning coplin jars? Does anyone store special stain dyes and solutions in coplin jars other than glass? If so, can you share with me your cleaning protocol and which coplin jars you use? We've had too many issues in our lab and washroom with chipped/broken glassware. Respectfully, Pamela Romundstad HT (ASCP), QIHCCM Lead Histology Technician Gundersen Health System H04-008 608-775-3139 From Karoleigh.Armstrong at ARMC.net Fri Sep 23 14:44:41 2016 From: Karoleigh.Armstrong at ARMC.net (Armstrong, Karoleigh T) Date: Fri, 23 Sep 2016 19:44:41 +0000 Subject: [Histonet] Unprocessing tissue Message-ID: Hi Caroline, This may sound a little crazy, but I was working on some mummified tissue back in the 80's, (Mommy is old), and to deal with the dried out tissue we would give them, 1. A quick .5 percent Sodium Hydroxide soak, 5minutes 2. Wash is running water, 1 minute 3. Soak in a 3 parts water 1 part Downey, yes Downey. 5 minutes 4. Step up to a 1/2 water, 1/2 Downey soak for 5- 10 minutes. 5. Wash with running water 2-3 minutes. Proscess your tissue as you usually would. They came out pretty darn good. KK Armstrong H.T. (ASCP) -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From dblackburn2000 at yahoo.com Sat Sep 24 12:51:38 2016 From: dblackburn2000 at yahoo.com (daniel blackburn) Date: Sat, 24 Sep 2016 17:51:38 +0000 (UTC) Subject: [Histonet] Re-embedment of tissue for TEM? References: <1969658633.4075973.1474739498957.ref@mail.yahoo.com> Message-ID: <1969658633.4075973.1474739498957@mail.yahoo.com> We had an unsuccessful embedding run, using Embed 812 resin (an Epon replacement, from Electron Microscopy Services). After curing in the oven, the resin blocks came out ?gummy? (flexible), perhaps due to a carryover of water in the paper labels. Would it be possible to re-embed the tissue, e.g., by running it through propylene oxide and 100% ethanol washes and back into new resin? We hope so, since the tissue is valuable to us. Thanks for any advice. From kdwyatt at vet.k-state.edu Sun Sep 25 08:00:57 2016 From: kdwyatt at vet.k-state.edu (Kristina Wyatt) Date: Sun, 25 Sep 2016 13:00:57 +0000 Subject: [Histonet] Kansas State Veterinary Diagnostic Lab is hiring a Research Assistant (Histology) Message-ID: The Kansas State Veterinary Diagnostic Laboratory is seeking applicants for a Research Assistant. The person in this position will perform histology duties related to service and research. Duties will include trimming (grossing) of fixed biopsy and necropsy tissues, embedding tissues in paraffin wax, cutting tissues on a microtome and preparing glass slides for H&E, special and immunohistochemical staining of the tissues in accordance with those QA/QC guidelines of an AAVLD accredited laboratory. Significant attention must be given to the KSVDL QMS (Quality Management System) and subsequent compliance. Working hours could vary as needed to meet customer demands for rapid turnaround times for biopsy submissions and could include working on Saturdays/weekends. To apply: http://careers.k-state.edu/cw/en-us/job/497583/research-assistant-veterinary-diagnostic-laboratory. Please submit an application along with a cover letter, resume and contact information for three professional references. Kansas State University is an Equal Opportunity Employer of individuals with disabilities and protected veterans and actively seeks diversity among its employees. Equal Employment Opportunity is the Law. In connection with your application for employment, Kansas State University may procure a Background Screen on you as part of the process of considering your candidacy as an employee. The Annual Security Report can be found at http://www.k-state.edu/studentlife/reportsandpolicies/. Kansas State University will provide a paper copy upon request. Cheers, Kristina Wyatt, M.A. Supervisor: Histopathology/Immunohistochemistry L216 Mosier Hall Kansas State Veterinary Diagnostic Laboratory 785-532-4464 kdwyatt at vet.k-state.edu From gmartin at marshallmedical.org Mon Sep 26 18:30:49 2016 From: gmartin at marshallmedical.org (Martin, Gary) Date: Mon, 26 Sep 2016 16:30:49 -0700 Subject: [Histonet] GMS Message-ID: <6ED9D4252F278841A0593D3D788AF24C366E5E51@mailsvr.MARSHMED.local> I am having difficulties with a stain I've done many times ...GMS. The light green is only staining the outer edges of the specimen. Any Ideas? Thanks Gary From mills at 3scan.com Mon Sep 26 18:49:14 2016 From: mills at 3scan.com (Caroline Miller) Date: Mon, 26 Sep 2016 16:49:14 -0700 Subject: [Histonet] Unprocessing tissue In-Reply-To: References: Message-ID: Not crazy at all, this is the kind of thing I was thinking someone would have out there :) I have used downey on hard tissue block faces before now too! thanks so much for all your advice histonet :) mills On Fri, Sep 23, 2016 at 12:44 PM, Armstrong, Karoleigh T via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Caroline, > > This may sound a little crazy, > > but I was working on some mummified tissue back in the 80's, (Mommy is > old), and to deal with the dried out tissue we would give them, > > 1. A quick .5 percent Sodium Hydroxide soak, 5minutes > > 2. Wash is running water, 1 minute > > 3. Soak in a 3 parts water 1 part Downey, yes Downey. 5 minutes > > 4. Step up to a 1/2 water, 1/2 Downey soak for 5- 10 minutes. > > 5. Wash with running water 2-3 minutes. > > Proscess your tissue as you usually would. > > They came out pretty darn good. > > KK Armstrong H.T. (ASCP) > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your computer. > Do not deliver, distribute or copy this message and do not disclose its > contents or take any action in reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From patpxs at gmail.com Tue Sep 27 11:28:25 2016 From: patpxs at gmail.com (P Sicurello) Date: Tue, 27 Sep 2016 09:28:25 -0700 Subject: [Histonet] Water for H&E Stainers? Message-ID: Good Morning Everyone, What type of water do you use with your automated H&E stainers? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From liz at premierlab.com Tue Sep 27 11:54:18 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Tue, 27 Sep 2016 10:54:18 -0600 Subject: [Histonet] Water for H&E Stainers? In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02BEEABA5B09@SBS2K8.premierlab.local> Paula We have ours hooked up to tap water. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 27, 2016 10:28 AM To: HistoNet Subject: [Histonet] Water for H&E Stainers? Good Morning Everyone, What type of water do you use with your automated H&E stainers? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen at parrishmed.com Tue Sep 27 12:10:05 2016 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Tue, 27 Sep 2016 13:10:05 -0400 Subject: [Histonet] Water for H&E Stainers? In-Reply-To: References: Message-ID: <450B7A81EDA0C54E97C53D60F00776C323E455E63B@isexstore03> Ours is hooked up to DI water. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 27, 2016 12:28 PM To: HistoNet Subject: [Histonet] Water for H&E Stainers? Good Morning Everyone, What type of water do you use with your automated H&E stainers? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From Mary.Leslie at tricore.org Tue Sep 27 12:13:54 2016 From: Mary.Leslie at tricore.org (Leslie, Mary) Date: Tue, 27 Sep 2016 17:13:54 +0000 Subject: [Histonet] Position announcement Message-ID: Histotechnologist needed M-F 1900 -0330 Tricore Reference Laboratories Albuquerque, NM Contact: laura.enriquez at tricore.org 505-938-8915 From gayle.callis at bresnan.net Tue Sep 27 12:19:17 2016 From: gayle.callis at bresnan.net (Gayle Callis) Date: Tue, 27 Sep 2016 11:19:17 -0600 Subject: [Histonet] Sandersons Rapid Bone stain chemistry and history Message-ID: <004a01d218e3$47811390$d6833ab0$@bresnan.net> You worte: RBS is the same as Methylene Blue, I believe. Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: -----Original Message----- From: Alicia Marie Ortega [mailto:alicia.ortega at colorado.edu ] Sent: Thursday, September 22, 2016 4:50 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Question on Sanderson Rapid Bone Stain Hello everyone, I am attempting to stain 30-40 micron thick undecalcified bone sections embedded in poly(methyl methacrylate) with Sanderson Rapid Bone Stain (RBS) with a Van Geison counter stain. My first attempt at this stain resulted in a very faint stain from the RBS (I could see very faint blue/green staining of osteoid/soft tissues) and a very intense dark pink stain from the counterstain. I was wondering if anyone knows of a way to intensify the staining of the RBS? I heated the stain to 55-60 degrees Celsius as recommended by the manufacturer prior to staining (with a 10 minute stain duration). Thank you in advance for your time and help. Sincerely, Alicia Ortega Postdoctoral Research Associate University of Colorado, Boulder *********************************************** Sandersons Rapid Bone Stain is oxidized methylene blue. It is actually a modified Stevenels blue, a polychromatic stain made by oxidizing methylene blue with potassium permanganate that produces the byproducts i.e. methylene violet, azure A, azure B, thionin, toluidine blue, thionin and possibly some other thiazine dyes .Stevenels blue was first used by Maniatopoulos et al to stain PMMA embedded, un-etched bone sections using a 60?C water bath and optional counterstaining with either Van Gieson or basic fuchsin. Cathy Sanderson (Mayton) found a better, easier way to make up Stevenels blue for large production. We found it tends to be a weaker stain unless you etch the bone with 0.5% formic acid using a sonicator for 1 minute, rinse with hot tap water very briefly, blot and look at the surface stained bone. Over aggressive water rinsing allows the stain to release from this mildly acid etched calcified bone. All the etching is doing is a gentle removal of calcium from only a few micrometers of bone surface and allow the stain to penetrate better. Acid etching intensified RBS staining but there are drawbacks but ways to deal with that. You can stain etched bone sections, view, photograph and then do a very brief (only a few quick dips), blot after counterstain, view and photograph the now counterstained section. Avoiding over rinsing keeps the RBS in etched bone section. If things don?t look right, merely polish the stain from surface and start over. You can counterstain Sanderson RBS stained (etched) bone section with the two mentioned counterstains, but it has to be done very briefly or the counterstain will differentiate RBS from the bone and be overly red. RBS, as used according to Sanderson and product brochure instructions, is for un-etched mineralize bone sections in PMMA or even EXAKT preparations. We preferred to acid etch, do the original Stevenels Blue instead of RBS, and get a much deeper stain on osteoid, and other bone components. Counterstaining was not done as it masked acid etched bone components excessively or removed them, The joy of using RBS is avoiding a long, tedious, and messy making up of Stevenels blue. One can also try to stain longer in RBS per brochure instructions to see if that deepens the staining. Old bone picks up the stain differently than young bone in terms of age of the animal. Different species can stain differently too. It is important to know that repeated heating of RBS (or Stevenels) allows the portassium permanganate to continue oxidizing the methylene blue, and also keeps raising the pH to be more alkaline. One should replenish or top off the stain, always filter it into a clean coplin jar before using or reusing this stain. Eventually the stain does not work very well and you need to start with new stock. We preferred to use a MacNeals tetrachrome combined with toluidine blue per Sterchi method for more brilliant staining of osteoid other bone components including cartilage. Take care Gayle Callis HTL/HT/MT(ASCP) From tbraud at holyredeemer.com Tue Sep 27 12:36:25 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 27 Sep 2016 17:36:25 +0000 Subject: [Histonet] water for stainers Message-ID: <48E053DDF6CE074DB6A7414BA05403F808DA66@HRHEX02-HOS.holyredeemer.local> Our Prisma is hooked up to tap water. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 3. Water for H&E Stainers? (P Sicurello) 4. Re: Water for H&E Stainers? (Elizabeth Chlipala) Message: 3 Date: Tue, 27 Sep 2016 09:28:25 -0700 From: P Sicurello Good Morning Everyone, What type of water do you use with your automated H&E stainers? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 From garethdavisyuma at gmail.com Tue Sep 27 12:45:25 2016 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Tue, 27 Sep 2016 10:45:25 -0700 Subject: [Histonet] H.pylori question Message-ID: Okay, so I know I have heard rumors about the H.pylori testing being affected by Medicare. We currently do the H. pylori IHC stain on all gastric biopsies. Has anyone heard that Medicare will change, and we will no longer be able to do it on all cases? The last lab I worked in, in Tennessee, stopped doing it all cases, because of the Medicare changes. But, here in Arizona, they are still doing it. What's the scoop? -- Ms. Gareth B. Davis, HT, QIHC (ASCPcm) Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From PREISZNE at mail.etsu.edu Tue Sep 27 13:21:12 2016 From: PREISZNE at mail.etsu.edu (Preiszner, Johanna) Date: Tue, 27 Sep 2016 18:21:12 +0000 Subject: [Histonet] buffered zn-formalin recipe needed Message-ID: Hi, For the life of me I can't locate a recipe for buffered zn-formalin, nor online or off. Could someone please help us out? Thanks, Hanna Preiszner ETSU/QCOM From mills at 3scan.com Tue Sep 27 13:23:47 2016 From: mills at 3scan.com (Caroline Miller) Date: Tue, 27 Sep 2016 11:23:47 -0700 Subject: [Histonet] GMS In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C366E5E51@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C366E5E51@mailsvr.MARSHMED.local> Message-ID: Maybe a fixation issue? Is this happening on all your slides, or just one set. Also, do you have a little acetic acid in the light green, I find that helps. I also find it washes out quickly and a fast dehydration is necessary. Very funny that you are having issues with the LG and not the silver, lol, histology can be so 'interesting' sometimes! mills On Mon, Sep 26, 2016 at 4:30 PM, Martin, Gary via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I am having difficulties with a stain I've done many times ...GMS. The > light green is only staining the outer edges of the specimen. Any Ideas? > > > Thanks > > Gary > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From kkienitz at orclinic.com Tue Sep 27 14:06:22 2016 From: kkienitz at orclinic.com (Kienitz, Kari) Date: Tue, 27 Sep 2016 19:06:22 +0000 Subject: [Histonet] H.pylori question In-Reply-To: References: Message-ID: Local Coverage Determinations (LCD) from the website for the Centers for Medicare & Medicaid Services (CMS). Please use the link below to access this policy. you may find this information useful. Look at the GI section. It specifically calls out the use of special stains for H.pylori. Reimbursement may be an issue for you. L35693 MolDX: Special Histochemical Stains and Immunohistochemical Stains Palmetto GBA(11302) http://www.cms.gov/medicare-coverage-database/details/lcd-details.aspx?LCDId=35693&ver=6&ContrId=229&ContrVer=1 N Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz at orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 27, 2016 10:45 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] H.pylori question Okay, so I know I have heard rumors about the H.pylori testing being affected by Medicare. We currently do the H. pylori IHC stain on all gastric biopsies. Has anyone heard that Medicare will change, and we will no longer be able to do it on all cases? The last lab I worked in, in Tennessee, stopped doing it all cases, because of the Medicare changes. But, here in Arizona, they are still doing it. What's the scoop? -- Ms. Gareth B. Davis, HT, QIHC (ASCPcm) Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum at uams.edu Tue Sep 27 14:55:03 2016 From: PAMarcum at uams.edu (Marcum, Pamela A) Date: Tue, 27 Sep 2016 19:55:03 +0000 Subject: [Histonet] ATRX Antibody Message-ID: <8856c26e8b844c50adb842ca38fd7958@MAIL13M2N1.ad.uams.edu> I am asking this question for our IHC Supervisor. She is looking for a source for the ATRX antibody as either an RUO or preferably IVD. They use the Ventana Ultras and Benchmarks so if anyone has a procedure that would be great. Pam ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From cfitz at 007group.com Tue Sep 27 16:17:08 2016 From: cfitz at 007group.com (cfitz) Date: Tue, 27 Sep 2016 14:17:08 -0700 Subject: [Histonet] Water for H&E Stainers? Message-ID: Our Prisma is connected to tap water with a prefilter that we change weekly.? CathyBritish Columbia? Sent from my Samsung Galaxy smartphone. -------- Original message --------From: Elizabeth Chlipala via Histonet Date: 2016-09-27 9:54 AM (GMT-08:00) To: P Sicurello , "'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)" Subject: Re: [Histonet] Water for H&E Stainers? Paula We have ours hooked up to tap water. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 27, 2016 10:28 AM To: HistoNet Subject: [Histonet] Water for H&E Stainers? Good Morning Everyone, What type of water do you use with your automated H&E stainers?? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time.? L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac at outlook.com Tue Sep 27 18:01:08 2016 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Tue, 27 Sep 2016 16:01:08 -0700 Subject: [Histonet] Water for H&E Stainers? In-Reply-To: References: Message-ID: Tap water is most often used in H&E staining for rinsing and sometimes blueing and can be very cost effective. I do suggest you use a filter prior to the water entering the instrument. The filter can eleviate one of the key concerns, contaminates. The typical contaminates/compounds can be: Inorganic ions: chlorides, nitrates, sulfates, sodium, calcium, iron Organic molecules: humic acids, phenols, tannins, pesticide residues Particles and colloids Microorganisms and their by-products Dissolved gases The other key concern is that the pH of the tap water may vary by location and even season. To change the color of hematoxylin stained nuclei from redish to blue, the pH should be 7 or higher. Most tap water is typically slightly acidic (pH of around 6.0 to 6.8), but this is much more alkaline than the pH of the haematoxylin, around pH 2.7. Rinsing for two to five minutes in running tap water will remove most of the excess mordant giving sharp blue nuclear staining. If you want sharper and better defined As my good friend Skip Brown states, a good H&E stain is "balance of coloration". Understand the chemistry and you control the balance. William DeSalvo, BS HTL(ASCP) > Date: Tue, 27 Sep 2016 14:17:08 -0700 > To: liz at premierlab.com; patpxs at gmail.com; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Water for H&E Stainers? > From: histonet at lists.utsouthwestern.edu > > Our Prisma is connected to tap water with a prefilter that we change weekly. > CathyBritish Columbia > > > Sent from my Samsung Galaxy smartphone. > -------- Original message --------From: Elizabeth Chlipala via Histonet Date: 2016-09-27 9:54 AM (GMT-08:00) To: P Sicurello , "'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)" Subject: Re: [Histonet] Water for H&E Stainers? > Paula > > We have ours hooked up to tap water. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz at premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, September 27, 2016 10:28 AM > To: HistoNet > Subject: [Histonet] Water for H&E Stainers? > > Good Morning Everyone, > > > > What type of water do you use with your automated H&E stainers? House or deionized/distilled? > > > > We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. L Thank you in advance. > > Sincerely, > > > > Paula > > > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shinwari_1 at yahoo.com Wed Sep 28 03:39:40 2016 From: shinwari_1 at yahoo.com (Nasir Abbas) Date: Wed, 28 Sep 2016 08:39:40 +0000 (UTC) Subject: [Histonet] Histonet Digest, Vol 154, Issue 22 In-Reply-To: References: Message-ID: <1743394579.715511.1475051980422@mail.yahoo.com> Dear Histoneters!Good DayMay I request you guys to share protocol of frozen tissues (mouse liver) for immunoflorescence assay. Lots of protocol are available online but i will highly appreciate the one that could lead me from tissue preparation towards staining procedure as I am really blank about frozen tissues and slides dealing. So I will be on standby for your response.?Thanks in advance. Sincerely?Nasir Abbas ---PhD FellowGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan Avenue?Science Park?Guangzhou, 510530,?Guangdong?P.R.ChinaCell: +86-1312 8296 614 On Wednesday, 28 September 2016, 1:12, "histonet-request at lists.utsouthwestern.edu" wrote: Send Histonet mailing list submissions to ??? histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. GMS (Martin, Gary) ? 2. Re: Unprocessing tissue (Caroline Miller) ? 3. Water for H&E Stainers? (P Sicurello) ? 4. Re: Water for H&E Stainers? (Elizabeth Chlipala) ---------------------------------------------------------------------- Message: 1 Date: Mon, 26 Sep 2016 16:30:49 -0700 From: "Martin, Gary" To: Subject: [Histonet] GMS Message-ID: ??? <6ED9D4252F278841A0593D3D788AF24C366E5E51 at mailsvr.MARSHMED.local> Content-Type: text/plain;??? charset="us-ascii" I am having difficulties with a stain I've done many times ...GMS. The light green is only staining the outer edges of the specimen. Any Ideas? Thanks Gary ------------------------------ Message: 2 Date: Mon, 26 Sep 2016 16:49:14 -0700 From: Caroline Miller To: "Armstrong, Karoleigh T" Cc: "histonet at lists.utsouthwestern.edu" ??? Subject: Re: [Histonet] Unprocessing tissue Message-ID: ??? Content-Type: text/plain; charset=UTF-8 Not crazy at all, this is the kind of thing I was thinking someone would have out there :) I have used downey on hard tissue block faces before now too! thanks so much for all your advice histonet :) mills On Fri, Sep 23, 2016 at 12:44 PM, Armstrong, Karoleigh T via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Caroline, > > This may sound a little crazy, > > but I was working on some mummified tissue back in the 80's, (Mommy is > old), and to deal with the dried out tissue we would give them, > > 1. A quick .5 percent Sodium Hydroxide soak, 5minutes > > 2. Wash is running water, 1 minute > > 3. Soak in a 3 parts water 1 part Downey, yes Downey. 5 minutes > > 4. Step up to a 1/2 water, 1/2 Downey soak for 5- 10 minutes. > > 5. Wash with running water 2-3 minutes. > > Proscess your tissue as you usually would. > > They came out pretty darn good. > > KK Armstrong H.T. (ASCP) > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your computer. > Do not deliver, distribute or copy this message and do not disclose its > contents or take any action in reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 3 Date: Tue, 27 Sep 2016 09:28:25 -0700 From: P Sicurello To: HistoNet Subject: [Histonet] Water for H&E Stainers? Message-ID: ??? Content-Type: text/plain; charset=UTF-8 Good Morning Everyone, What type of water do you use with your automated H&E stainers?? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time.? L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer. ------------------------------ Message: 4 Date: Tue, 27 Sep 2016 10:54:18 -0600 From: Elizabeth Chlipala To: P Sicurello , ??? "'histonet at lists.utsouthwestern.edu' ??? (histonet at lists.utsouthwestern.edu)" ??? Subject: Re: [Histonet] Water for H&E Stainers? Message-ID: ??? <14E2C6176416974295479C64A11CB9AE02BEEABA5B09 at SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Paula We have ours hooked up to tap water. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 27, 2016 10:28 AM To: HistoNet Subject: [Histonet] Water for H&E Stainers? Good Morning Everyone, What type of water do you use with your automated H&E stainers?? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time.? L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 154, Issue 22 ***************************************** From fnawaz at uhcc.com Wed Sep 28 09:02:38 2016 From: fnawaz at uhcc.com (Farah Nawaz) Date: Wed, 28 Sep 2016 14:02:38 +0000 Subject: [Histonet] HT grossing competencies Message-ID: Hello: I am a new AP supervisor and I am looking to add grossing duties to our HTs. They are currently being trained here in our lab. Can someone please share an example of non-pathologist grossing annual competency checklist with me? I just need a reference to create our own competency checklist for our HTs that gross. Thank you, Farah Nawaz, CT(ASCP), MA-Mgmt Anatomic Pathology Supervisor Union Hospital of Cecil County Office: 410-392-7084 Fax: 443-674-1596 fnawaz at uhcc.com CONFIDENTIALITY STATEMENT. This email and any attachment is for the sole use of the intended recipient and may contain private, confidential and/or privileged information that may be subject to Union Hospital of Cecil County internal policies. If you are not the intended recipient, any dissemination, distribution or copying is strictly prohibited. If you have received this transmission in error, please notify Union Hospital of Cecil County immediately by return email or by email to Administrator at uhcc.com and delete the message and all copies, printouts and attachments from your system. From phebert at shrinenet.org Wed Sep 28 09:40:01 2016 From: phebert at shrinenet.org (Hebert, Pamela) Date: Wed, 28 Sep 2016 14:40:01 +0000 Subject: [Histonet] looking for a cryostat service tech. in the Houston area. Message-ID: I am looking for a cryostat service tech in the Houston/Galveston area to do a PM. Any recommendations would be appreciated. Pam Hebert, HT(ASCP) Shriners Hospitals for Children- Galveston, TX From lori.garcia at medtronic.com Wed Sep 28 11:32:56 2016 From: lori.garcia at medtronic.com (Garcia, Lori, M.Sc.) Date: Wed, 28 Sep 2016 16:32:56 +0000 Subject: [Histonet] EXAKT band saw blade Message-ID: Looking for an EXAKT band saw blade for a colleague due to a backorder. If anyone has one to loan out or sell, please contact me. Thanks, Lori [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From mills at 3scan.com Wed Sep 28 12:21:45 2016 From: mills at 3scan.com (Caroline Miller) Date: Wed, 28 Sep 2016 10:21:45 -0700 Subject: [Histonet] Material for size calibration in blocks Message-ID: Hi Histonet, I am looking for a material of a precise size that I can place in my paraffin or resin blocks to act as size calibrators when we put the blocks on our automated serial sectioning and imaging robot (see www.3scan.com). I would like to do this without processing the tissue, so I suppose I am actually asking has anyone successfully cut any materials without any processing? Thinking of brush bristles, but worried they will not cut. Also thought of carbon fibre, but possibly too hard and could damage our diamond blade. Thanks for any advice in this space. yours, mills -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From amosbrooks at gmail.com Wed Sep 28 12:41:09 2016 From: amosbrooks at gmail.com (Amos Brooks) Date: Wed, 28 Sep 2016 13:41:09 -0400 Subject: [Histonet] buffered zn-formalin recipe needed Message-ID: Hi Johanna, The Histonet Archives are your friend. In 2009, Gayle Callis posted a great formulation that being the neo-Luddite that I am I printed and have a copy of here for just such an occasion... http://lists.utsouthwestern.edu/mailman/htdig/histonet/2009-October/046985.html Zinc Fixative (JB Fixative or ZSF) 0.1M Tris Buffer, pH 7.4 Tris Base -------------------------------- 12.1 g (TRIZMA) 1N HCL ----------------------------------- 81.5 ml Distilled water -------------------------- 900 ml Mix to dissolve. Adjust pH to 7.4 Zinc Fixative Calcium Acetate ---------------------- 0.5 g Zinc Acetate -------------------------- 5.0 g Zinc Chloride -------------------------- 5.0 g 0.1M Tris Buffer made above ------ 1000 ml Mix to dissolve. The final pH will be approximately 6.5-7.0. Do not readjust the pH, as this will cause the zinc to come out of solution. Store Zinc Fixative at room temperature. Fix tissues for 24 to 48 hours. Fixation longer than 48 hours may make the tissue brittle and difficult to cut. Good Luck, Amos On Wed, Sep 28, 2016 at 12:33 PM, wrote: > Message: 6 > Date: Tue, 27 Sep 2016 18:21:12 +0000 > From: "Preiszner, Johanna" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] buffered zn-formalin recipe needed > Message-ID: > namprd07.prod.outlook.com> > > Content-Type: text/plain; charset="iso-8859-1" > > Hi, > > > For the life of me I can't locate a recipe for buffered zn-formalin, nor > online or off. > > > Could someone please help us out? > > > Thanks, > > Hanna Preiszner > > ETSU/QCOM > From blayjorge at gmail.com Wed Sep 28 12:56:25 2016 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Wed, 28 Sep 2016 13:56:25 -0400 Subject: [Histonet] STEM and the ARTS Message-ID: STEM and the ARTS Dear Colleagues: I am putting together a little presentation for a regional workshop on *STEM and the ARTS *(details after the PS). While I am emphasizing ecology/environmental sciences, if you have experience in this topic (anecdotes are OK) - regardless of the area (e.g. histology and related sciences), and wish to collaborate, please email me directly (not to the list) no later than this weekend. blayjorge at gmail.com Gratefully, Jorge P.S. Apologies for potential duplicate emails. Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers. com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From tfraser at olympicmedical.org Wed Sep 28 15:04:34 2016 From: tfraser at olympicmedical.org (Tasha Fraser) Date: Wed, 28 Sep 2016 20:04:34 +0000 Subject: [Histonet] Grossing HT Message-ID: I was hoping to find out if anyone else has come across this problem. I'm a HT and have been since 2006. Our lab would now like us HT's to start grossing for the Pathologist. As I've been doing my research I see that we are not qualified to do the grossing under the CLIA regulations. Both HT's here at my hospital got our HT certification after online schooling through Harford Community College. This schooling was for certification only not an AAS. Has anyone else out there come across this or does anyone have any suggestions on the easiest way to get qualified to do the grossing. Tasha Fraser, HT (ASCP) Olympic Medical Center Port Angeles, WA 98362 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. From cmmathis at wakehealth.edu Wed Sep 28 16:04:03 2016 From: cmmathis at wakehealth.edu (Cathy M. Mathis/Comparative Medicine) Date: Wed, 28 Sep 2016 21:04:03 +0000 Subject: [Histonet] Questions concerning Leica Bond RX Message-ID: <4816F916B11AEC4E92A0D01372F573E2010FFCDF38@EXCHDB8.medctr.ad.wfubmc.edu> Hello Histo-netters! I have a Bond RX and before I try to recreate the wheel, I thought I would ask some experts. Has anyone used a goat primary on this instrument and if so how? Also, has anyone had success with TUNEL on this instrument and if so, could you share your knowledge? Thank you all for your time, Cathy Cathy M. Mathis Laboratory Technician IV Department of Pathology - Section on Comparative Medicine Clarkson Campus? Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.1538 cmmathis at wakehealth.edu -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Wednesday, September 28, 2016 12:33 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 154, Issue 23 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Water for H&E Stainers? (Hannen, Valerie) 2. Position announcement (Leslie, Mary) 3. Sandersons Rapid Bone stain chemistry and history (Gayle Callis) 4. Re: water for stainers (Terri Braud) 5. H.pylori question (Gareth Davis) 6. buffered zn-formalin recipe needed (Preiszner, Johanna) 7. Re: GMS (Caroline Miller) 8. Re: H.pylori question (Kienitz, Kari) 9. ATRX Antibody (Marcum, Pamela A) 10. Re: Water for H&E Stainers? (cfitz) 11. Re: Water for H&E Stainers? (WILLIAM DESALVO) 12. Re: Histonet Digest, Vol 154, Issue 22 (Nasir Abbas) 13. HT grossing competencies (Farah Nawaz) 14. looking for a cryostat service tech. in the Houston area. (Hebert, Pamela) 15. EXAKT band saw blade (Garcia, Lori, M.Sc.) ---------------------------------------------------------------------- Message: 1 Date: Tue, 27 Sep 2016 13:10:05 -0400 From: "Hannen, Valerie" To: 'P Sicurello' Cc: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Water for H&E Stainers? Message-ID: <450B7A81EDA0C54E97C53D60F00776C323E455E63B at isexstore03> Content-Type: text/plain; charset="us-ascii" Ours is hooked up to DI water. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 27, 2016 12:28 PM To: HistoNet Subject: [Histonet] Water for H&E Stainers? Good Morning Everyone, What type of water do you use with your automated H&E stainers? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== ------------------------------ Message: 2 Date: Tue, 27 Sep 2016 17:13:54 +0000 From: "Leslie, Mary" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Position announcement Message-ID: Content-Type: text/plain; charset="us-ascii" Histotechnologist needed M-F 1900 -0330 Tricore Reference Laboratories Albuquerque, NM Contact: laura.enriquez at tricore.org 505-938-8915 ------------------------------ Message: 3 Date: Tue, 27 Sep 2016 11:19:17 -0600 From: "Gayle Callis" To: "Histonet" Subject: [Histonet] Sandersons Rapid Bone stain chemistry and history Message-ID: <004a01d218e3$47811390$d6833ab0$@bresnan.net> Content-Type: text/plain; charset="iso-8859-1" You worte: RBS is the same as Methylene Blue, I believe. Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: -----Original Message----- From: Alicia Marie Ortega [mailto:alicia.ortega at colorado.edu ] Sent: Thursday, September 22, 2016 4:50 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Question on Sanderson Rapid Bone Stain Hello everyone, I am attempting to stain 30-40 micron thick undecalcified bone sections embedded in poly(methyl methacrylate) with Sanderson Rapid Bone Stain (RBS) with a Van Geison counter stain. My first attempt at this stain resulted in a very faint stain from the RBS (I could see very faint blue/green staining of osteoid/soft tissues) and a very intense dark pink stain from the counterstain. I was wondering if anyone knows of a way to intensify the staining of the RBS? I heated the stain to 55-60 degrees Celsius as recommended by the manufacturer prior to staining (with a 10 minute stain duration). Thank you in advance for your time and help. Sincerely, Alicia Ortega Postdoctoral Research Associate University of Colorado, Boulder *********************************************** Sandersons Rapid Bone Stain is oxidized methylene blue. It is actually a modified Stevenels blue, a polychromatic stain made by oxidizing methylene blue with potassium permanganate that produces the byproducts i.e. methylene violet, azure A, azure B, thionin, toluidine blue, thionin and possibly some other thiazine dyes .Stevenels blue was first used by Maniatopoulos et al to stain PMMA embedded, un-etched bone sections using a 60?C water bath and optional counterstaining with either Van Gieson or basic fuchsin. Cathy Sanderson (Mayton) found a better, easier way to make up Stevenels blue for large production. We found it tends to be a weaker stain unless you etch the bone with 0.5% formic acid using a sonicator for 1 minute, rinse with hot tap water very briefly, blot and look at the surface stained bone. Over aggressive water rinsing allows the stain to release from this mildly acid etched calcified bone. All the etching is doing is a gentle removal of calcium from only a few micrometers of bone surface and allow the stain to penetrate better. Acid etching intensified RBS staining but there are drawbacks but ways to deal with that. You can stain etched bone sections, view, photograph and then do a very brief (only a few quick dips), blot after counterstain, view and photograph the now counterstained section. Avoiding over rinsing keeps the RBS in etched bone section. If things don?t look right, merely polish the stain from surface and start over. You can counterstain Sanderson RBS stained (etched) bone section with the two mentioned counterstains, but it has to be done very briefly or the counterstain will differentiate RBS from the bone and be overly red. RBS, as used according to Sanderson and product brochure instructions, is for un-etched mineralize bone sections in PMMA or even EXAKT preparations. We preferred to acid etch, do the original Stevenels Blue instead of RBS, and get a much deeper stain on osteoid, and other bone components. Counterstaining was not done as it masked acid etched bone components excessively or removed them, The joy of using RBS is avoiding a long, tedious, and messy making up of Stevenels blue. One can also try to stain longer in RBS per brochure instructions to see if that deepens the staining. Old bone picks up the stain differently than young bone in terms of age of the animal. Different species can stain differently too. It is important to know that repeated heating of RBS (or Stevenels) allows the portassium permanganate to continue oxidizing the methylene blue, and also keeps raising the pH to be more alkaline. One should replenish or top off the stain, always filter it into a clean coplin jar before using or reusing this stain. Eventually the stain does not work very well and you need to start with new stock. We preferred to use a MacNeals tetrachrome combined with toluidine blue per Sterchi method for more brilliant staining of osteoid other bone components including cartilage. Take care Gayle Callis HTL/HT/MT(ASCP) ------------------------------ Message: 4 Date: Tue, 27 Sep 2016 17:36:25 +0000 From: "Terri Braud" To: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] water for stainers Message-ID: <48E053DDF6CE074DB6A7414BA05403F808DA66 at HRHEX02-HOS.holyredeemer.local> Content-Type: text/plain; charset="us-ascii" Our Prisma is hooked up to tap water. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 3. Water for H&E Stainers? (P Sicurello) 4. Re: Water for H&E Stainers? (Elizabeth Chlipala) Message: 3 Date: Tue, 27 Sep 2016 09:28:25 -0700 From: P Sicurello Good Morning Everyone, What type of water do you use with your automated H&E stainers? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 ------------------------------ Message: 5 Date: Tue, 27 Sep 2016 10:45:25 -0700 From: Gareth Davis To: histonet at lists.utsouthwestern.edu Subject: [Histonet] H.pylori question Message-ID: Content-Type: text/plain; charset=UTF-8 Okay, so I know I have heard rumors about the H.pylori testing being affected by Medicare. We currently do the H. pylori IHC stain on all gastric biopsies. Has anyone heard that Medicare will change, and we will no longer be able to do it on all cases? The last lab I worked in, in Tennessee, stopped doing it all cases, because of the Medicare changes. But, here in Arizona, they are still doing it. What's the scoop? -- Ms. Gareth B. Davis, HT, QIHC (ASCPcm) Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 ------------------------------ Message: 6 Date: Tue, 27 Sep 2016 18:21:12 +0000 From: "Preiszner, Johanna" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] buffered zn-formalin recipe needed Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, For the life of me I can't locate a recipe for buffered zn-formalin, nor online or off. Could someone please help us out? Thanks, Hanna Preiszner ETSU/QCOM ------------------------------ Message: 7 Date: Tue, 27 Sep 2016 11:23:47 -0700 From: Caroline Miller To: "Martin, Gary" Cc: "Histonet at Lists. Edu" Subject: Re: [Histonet] GMS Message-ID: Content-Type: text/plain; charset=UTF-8 Maybe a fixation issue? Is this happening on all your slides, or just one set. Also, do you have a little acetic acid in the light green, I find that helps. I also find it washes out quickly and a fast dehydration is necessary. Very funny that you are having issues with the LG and not the silver, lol, histology can be so 'interesting' sometimes! mills On Mon, Sep 26, 2016 at 4:30 PM, Martin, Gary via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I am having difficulties with a stain I've done many times ...GMS. The > light green is only staining the outer edges of the specimen. Any Ideas? > > > Thanks > > Gary > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 8 Date: Tue, 27 Sep 2016 19:06:22 +0000 From: "Kienitz, Kari" To: Gareth Davis , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] H.pylori question Message-ID: Content-Type: text/plain; charset="us-ascii" Local Coverage Determinations (LCD) from the website for the Centers for Medicare & Medicaid Services (CMS). Please use the link below to access this policy. you may find this information useful. Look at the GI section. It specifically calls out the use of special stains for H.pylori. Reimbursement may be an issue for you. L35693 MolDX: Special Histochemical Stains and Immunohistochemical Stains Palmetto GBA(11302) http://www.cms.gov/medicare-coverage-database/details/lcd-details.aspx?LCDId=35693&ver=6&ContrId=229&ContrVer=1 N Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz at orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: Gareth Davis via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 27, 2016 10:45 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] H.pylori question Okay, so I know I have heard rumors about the H.pylori testing being affected by Medicare. We currently do the H. pylori IHC stain on all gastric biopsies. Has anyone heard that Medicare will change, and we will no longer be able to do it on all cases? The last lab I worked in, in Tennessee, stopped doing it all cases, because of the Medicare changes. But, here in Arizona, they are still doing it. What's the scoop? -- Ms. Gareth B. Davis, HT, QIHC (ASCPcm) Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 27 Sep 2016 19:55:03 +0000 From: "Marcum, Pamela A" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] ATRX Antibody Message-ID: <8856c26e8b844c50adb842ca38fd7958 at MAIL13M2N1.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" I am asking this question for our IHC Supervisor. She is looking for a source for the ATRX antibody as either an RUO or preferably IVD. They use the Ventana Ultras and Benchmarks so if anyone has a procedure that would be great. Pam ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 10 Date: Tue, 27 Sep 2016 14:17:08 -0700 From: cfitz To: Elizabeth Chlipala , P Sicurello , "'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)" Subject: Re: [Histonet] Water for H&E Stainers? Message-ID: Content-Type: text/plain; charset=utf-8 Our Prisma is connected to tap water with a prefilter that we change weekly.? CathyBritish Columbia? Sent from my Samsung Galaxy smartphone. -------- Original message --------From: Elizabeth Chlipala via Histonet Date: 2016-09-27 9:54 AM (GMT-08:00) To: P Sicurello , "'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)" Subject: Re: [Histonet] Water for H&E Stainers? Paula We have ours hooked up to tap water. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 27, 2016 10:28 AM To: HistoNet Subject: [Histonet] Water for H&E Stainers? Good Morning Everyone, What type of water do you use with your automated H&E stainers?? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time.? L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Tue, 27 Sep 2016 16:01:08 -0700 From: WILLIAM DESALVO To: cfitz , Liz Chlipala , P Sicurello , histonet Subject: Re: [Histonet] Water for H&E Stainers? Message-ID: Content-Type: text/plain; charset="iso-8859-1" Tap water is most often used in H&E staining for rinsing and sometimes blueing and can be very cost effective. I do suggest you use a filter prior to the water entering the instrument. The filter can eleviate one of the key concerns, contaminates. The typical contaminates/compounds can be: Inorganic ions: chlorides, nitrates, sulfates, sodium, calcium, iron Organic molecules: humic acids, phenols, tannins, pesticide residues Particles and colloids Microorganisms and their by-products Dissolved gases The other key concern is that the pH of the tap water may vary by location and even season. To change the color of hematoxylin stained nuclei from redish to blue, the pH should be 7 or higher. Most tap water is typically slightly acidic (pH of around 6.0 to 6.8), but this is much more alkaline than the pH of the haematoxylin, around pH 2.7. Rinsing for two to five minutes in running tap water will remove most of the excess mordant giving sharp blue nuclear staining. If you want sharper and better defined As my good friend Skip Brown states, a good H&E stain is "balance of coloration". Understand the chemistry and you control the balance. William DeSalvo, BS HTL(ASCP) > Date: Tue, 27 Sep 2016 14:17:08 -0700 > To: liz at premierlab.com; patpxs at gmail.com; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Water for H&E Stainers? > From: histonet at lists.utsouthwestern.edu > > Our Prisma is connected to tap water with a prefilter that we change weekly. > CathyBritish Columbia > > > Sent from my Samsung Galaxy smartphone. > -------- Original message --------From: Elizabeth Chlipala via Histonet Date: 2016-09-27 9:54 AM (GMT-08:00) To: P Sicurello , "'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)" Subject: Re: [Histonet] Water for H&E Stainers? > Paula > > We have ours hooked up to tap water. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz at premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, September 27, 2016 10:28 AM > To: HistoNet > Subject: [Histonet] Water for H&E Stainers? > > Good Morning Everyone, > > > > What type of water do you use with your automated H&E stainers? House or deionized/distilled? > > > > We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. L Thank you in advance. > > Sincerely, > > > > Paula > > > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 28 Sep 2016 08:39:40 +0000 (UTC) From: Nasir Abbas To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Histonet Digest, Vol 154, Issue 22 Message-ID: <1743394579.715511.1475051980422 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Dear Histoneters!Good DayMay I request you guys to share protocol of frozen tissues (mouse liver) for immunoflorescence assay. Lots of protocol are available online but i will highly appreciate the one that could lead me from tissue preparation towards staining procedure as I am really blank about frozen tissues and slides dealing. So I will be on standby for your response.?Thanks in advance. Sincerely?Nasir Abbas ---PhD FellowGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan Avenue?Science Park?Guangzhou, 510530,?Guangdong?P.R.ChinaCell: +86-1312 8296 614 On Wednesday, 28 September 2016, 1:12, "histonet-request at lists.utsouthwestern.edu" wrote: Send Histonet mailing list submissions to ??? histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. GMS (Martin, Gary) ? 2. Re: Unprocessing tissue (Caroline Miller) ? 3. Water for H&E Stainers? (P Sicurello) ? 4. Re: Water for H&E Stainers? (Elizabeth Chlipala) ---------------------------------------------------------------------- Message: 1 Date: Mon, 26 Sep 2016 16:30:49 -0700 From: "Martin, Gary" To: Subject: [Histonet] GMS Message-ID: ??? <6ED9D4252F278841A0593D3D788AF24C366E5E51 at mailsvr.MARSHMED.local> Content-Type: text/plain;??? charset="us-ascii" I am having difficulties with a stain I've done many times ...GMS. The light green is only staining the outer edges of the specimen. Any Ideas? Thanks Gary ------------------------------ Message: 2 Date: Mon, 26 Sep 2016 16:49:14 -0700 From: Caroline Miller To: "Armstrong, Karoleigh T" Cc: "histonet at lists.utsouthwestern.edu" ??? Subject: Re: [Histonet] Unprocessing tissue Message-ID: ??? Content-Type: text/plain; charset=UTF-8 Not crazy at all, this is the kind of thing I was thinking someone would have out there :) I have used downey on hard tissue block faces before now too! thanks so much for all your advice histonet :) mills On Fri, Sep 23, 2016 at 12:44 PM, Armstrong, Karoleigh T via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Caroline, > > This may sound a little crazy, > > but I was working on some mummified tissue back in the 80's, (Mommy is > old), and to deal with the dried out tissue we would give them, > > 1. A quick .5 percent Sodium Hydroxide soak, 5minutes > > 2. Wash is running water, 1 minute > > 3. Soak in a 3 parts water 1 part Downey, yes Downey. 5 minutes > > 4. Step up to a 1/2 water, 1/2 Downey soak for 5- 10 minutes. > > 5. Wash with running water 2-3 minutes. > > Proscess your tissue as you usually would. > > They came out pretty darn good. > > KK Armstrong H.T. (ASCP) > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your computer. > Do not deliver, distribute or copy this message and do not disclose its > contents or take any action in reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 3 Date: Tue, 27 Sep 2016 09:28:25 -0700 From: P Sicurello To: HistoNet Subject: [Histonet] Water for H&E Stainers? Message-ID: ??? Content-Type: text/plain; charset=UTF-8 Good Morning Everyone, What type of water do you use with your automated H&E stainers?? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time.? L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer. ------------------------------ Message: 4 Date: Tue, 27 Sep 2016 10:54:18 -0600 From: Elizabeth Chlipala To: P Sicurello , ??? "'histonet at lists.utsouthwestern.edu' ??? (histonet at lists.utsouthwestern.edu)" ??? Subject: Re: [Histonet] Water for H&E Stainers? Message-ID: ??? <14E2C6176416974295479C64A11CB9AE02BEEABA5B09 at SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Paula We have ours hooked up to tap water. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 27, 2016 10:28 AM To: HistoNet Subject: [Histonet] Water for H&E Stainers? Good Morning Everyone, What type of water do you use with your automated H&E stainers?? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H&E stainers at this time.? L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 154, Issue 22 ***************************************** ------------------------------ Message: 13 Date: Wed, 28 Sep 2016 14:02:38 +0000 From: Farah Nawaz To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] HT grossing competencies Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello: I am a new AP supervisor and I am looking to add grossing duties to our HTs. They are currently being trained here in our lab. Can someone please share an example of non-pathologist grossing annual competency checklist with me? I just need a reference to create our own competency checklist for our HTs that gross. Thank you, Farah Nawaz, CT(ASCP), MA-Mgmt Anatomic Pathology Supervisor Union Hospital of Cecil County Office: 410-392-7084 Fax: 443-674-1596 fnawaz at uhcc.com CONFIDENTIALITY STATEMENT. This email and any attachment is for the sole use of the intended recipient and may contain private, confidential and/or privileged information that may be subject to Union Hospital of Cecil County internal policies. If you are not the intended recipient, any dissemination, distribution or copying is strictly prohibited. If you have received this transmission in error, please notify Union Hospital of Cecil County immediately by return email or by email to Administrator at uhcc.com and delete the message and all copies, printouts and attachments from your system. ------------------------------ Message: 14 Date: Wed, 28 Sep 2016 14:40:01 +0000 From: "Hebert, Pamela" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] looking for a cryostat service tech. in the Houston area. Message-ID: Content-Type: text/plain; charset="us-ascii" I am looking for a cryostat service tech in the Houston/Galveston area to do a PM. Any recommendations would be appreciated. Pam Hebert, HT(ASCP) Shriners Hospitals for Children- Galveston, TX ------------------------------ Message: 15 Date: Wed, 28 Sep 2016 16:32:56 +0000 From: "Garcia, Lori, M.Sc." To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] EXAKT band saw blade Message-ID: Content-Type: text/plain; charset="us-ascii" Looking for an EXAKT band saw blade for a colleague due to a backorder. If anyone has one to loan out or sell, please contact me. Thanks, Lori [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 154, Issue 23 ***************************************** From relia1 at earthlink.net Thu Sep 29 07:21:10 2016 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 29 Sep 2016 08:21:10 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin 9/29/2016 Exciting New Opportunities in NC, CA, VA, WI, TN and AZ Message-ID: <000001d21a4b$f6baeac0$e430c040$@earthlink.net> Hi Histonetters! ComicCon and an Elvis Impersonator and so much more! The NSH/SC in Long Beach was GREAT!! I enjoyed making new friends, reconnecting with old friends and learning new things. I saw superheroes, alien monsters and an Elvis impersonator who asked me to be his back up dancer!! But the most fun of all I worked the registration desk with some really fun histotechs! I posted some pictures on Facebook, Twitter and Instagram with the Hashtag #NSHSC. If you get a chance check them out!! I really encourage everyone to go to meetings when they can. If you can?t make the national meeting try to make it to a regional or state meeting. The camaraderie and educational opportunities are phenomenal. Next year the convention will be held in my home town! Orlando, FL!! Hope to see you there!!! My phone never stopped ringing while I was gone!! All of my positions are full time permanent positions. My clients offer excellent compensation, benefits and relocation assistance. **And they are ready to interview and hire right away!** Here Is A List Of My Current Openings: Lead Histology Tech ? Run your own brand new lab in San Diego, CA!! Director of Laboratory Services ? Turlock, CA Histotech ? Roanoke, VA IHC Tech ? Modesto, CA Histotech ? Modesto, CA Histotech ? Manitowoc, WI Histotech ? Asheville, NC Histology Tech ? Kingsport, TN Histology Tech - Flagstaff, AZ Of course I can?t put all the information about these opportunities in an e-mail. So if you or anyone you know might be interested in hearing more about any of these positions or want help with a job search in another area please contact me. I can be reached at 866-607-3542 or on my cell at 407-353-5070 or relia1 at earthlink.net. Remember it never hurts to look. Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From judi at medialab.com Thu Sep 29 11:27:13 2016 From: judi at medialab.com (Judi Bennett) Date: Thu, 29 Sep 2016 12:27:13 -0400 Subject: [Histonet] Histology, Cytology, and Cytogenetics Authors Wanted!! Message-ID: *MediaLab* is actively seeking authors to write online *HISTOLOGY*, *CYTOLOGY*, and *CYTOGENETIC** courses*! MediaLab is a leading publisher of online continuing education (CE) courses for laboratory professionals. Our online products are used at more than 2,000 laboratories and CLS and Histology programs worldwide. This is a great opportunity to: - Gain* resume-boosting publishing experience* - *Earn honorariums* for your participation - Fill the *critical need for quality histology CE credits* Authors can take advantage of MediaLab's online CourseBuilder to *write courses anytime, anywhere*. CourseBuilder is easy to use, with an intuitive interface similar to Microsoft Word or PowerPoint. Authors can quickly create content pages, practice questions, and exam questions, and upload relevant images. Courses developed by MediaLab are *featured on our websites, MediaLab.com and LabCE.com*. To learn more about becoming a MediaLab author for histology, cytology, or cytogenetics courses, visit our online information page at https://www.medialabinc.net/authors.aspx. Please contact Judi Bennett at judi at medialab.com or call 877-776-8460, ext. 721. Looking forward to talking with you! Judi -- Judi Bennett, MT, BSM Program Director - MediaLab, Inc. e-mail judi at medialab.com Phone (877) 776-8460 ext. 721 cell phone 404-915-2999 fax (678) 401-0284 From mills at 3scan.com Thu Sep 29 12:23:12 2016 From: mills at 3scan.com (Caroline Miller) Date: Thu, 29 Sep 2016 10:23:12 -0700 Subject: [Histonet] buffered zn-formalin recipe needed In-Reply-To: References: Message-ID: Histonet archives FTW! Also IHC world is your friend: http://www.ihcworld.com/_protocols/histology/zinc_fixative.htm (and whilst i am here I shall also recommend http://stainsfile.info/StainsFile/jindex.html as a great friend too)! mills On Wed, Sep 28, 2016 at 10:41 AM, Amos Brooks via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Johanna, > The Histonet Archives are your friend. In 2009, Gayle Callis posted a > great formulation that being the neo-Luddite that I am I printed and have a > copy of here for just such an occasion... > > http://lists.utsouthwestern.edu/mailman/htdig/histonet/ > 2009-October/046985.html > > Zinc Fixative (JB Fixative or ZSF) > > 0.1M Tris Buffer, pH 7.4 > Tris Base -------------------------------- 12.1 g (TRIZMA) > 1N HCL ----------------------------------- 81.5 ml > Distilled water -------------------------- 900 ml > > Mix to dissolve. Adjust pH to 7.4 > Zinc Fixative > Calcium Acetate ---------------------- 0.5 g > Zinc Acetate -------------------------- 5.0 g > Zinc Chloride -------------------------- 5.0 g > 0.1M Tris Buffer made above ------ 1000 ml > Mix to dissolve. The final pH will be approximately 6.5-7.0. Do not > readjust > the pH, as this will cause the zinc to come out of solution. Store Zinc > Fixative at room temperature. Fix tissues for 24 to 48 hours. Fixation > longer than 48 hours may make the tissue brittle and difficult to cut. > > Good Luck, > > Amos > > > > > On Wed, Sep 28, 2016 at 12:33 PM, utsouthwestern.edu > > wrote: > > > Message: 6 > > Date: Tue, 27 Sep 2016 18:21:12 +0000 > > From: "Preiszner, Johanna" > > To: "histonet at lists.utsouthwestern.edu" > > > > Subject: [Histonet] buffered zn-formalin recipe needed > > Message-ID: > > > namprd07.prod.outlook.com> > > > > Content-Type: text/plain; charset="iso-8859-1" > > > > Hi, > > > > > > For the life of me I can't locate a recipe for buffered zn-formalin, nor > > online or off. > > > > > > Could someone please help us out? > > > > > > Thanks, > > > > Hanna Preiszner > > > > ETSU/QCOM > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From j.benavides at eae.csic.es Fri Sep 30 06:35:59 2016 From: j.benavides at eae.csic.es (Julio Benavides) Date: Fri, 30 Sep 2016 13:35:59 +0200 Subject: [Histonet] Buffered formalin substitution In-Reply-To: References: Message-ID: <20160930133559.Horde.eMJ-oxJdX7QHExt21X45hw8@webmail.csic.es> Hi there, The health officer of our institute has risen (again!) the issue of substituting buffered formalin for some other less hazardous fixative . I would like to know you opinion, and experience, in such matter. Is it even possible? Have you successfully done it? In that case, which samples you normally handle? We are a research institute, doing several ruminant PMs a week, so big chunks of tissue to fix. Then, we normally do IHCs with a variety of antibodies, depending on the project. My worries are that, in case buffered formalin could be substituted, you can never be sure about IHC and maybe antibodies that were working before stop doing it. Very many thanks for your thoughts and time in this issue. Greatly appreciated! Cheers Julio From rjbuesa at yahoo.com Fri Sep 30 09:29:28 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 30 Sep 2016 14:29:28 +0000 (UTC) Subject: [Histonet] Buffered formalin substitution In-Reply-To: <20160930133559.Horde.eMJ-oxJdX7QHExt21X45hw8@webmail.csic.es> References: <20160930133559.Horde.eMJ-oxJdX7QHExt21X45hw8@webmail.csic.es> Message-ID: <843965740.3208201.1475245768151@mail.yahoo.com> Julio:Unfortunately NBF is the OVERALL best fixative there is. ANY substitute will be good for some things and not that good?for others. Under those circumstances what to do? Simply use LESS amounts of formalin, do it safely keeping to a minimum its exposure.Under separate cover I am sending you 2 articles I published on the subject.Ren? On Friday, September 30, 2016 8:04 AM, Julio Benavides via Histonet wrote: Hi there, The health officer of our institute has risen (again!) the issue of? substituting buffered formalin for some other less hazardous fixative . I would like to know you opinion, and experience, in such matter. Is? it even possible? Have you successfully done it? In that case, which? samples you normally handle? We are a research institute, doing? several ruminant PMs a week, so big chunks of tissue to fix. Then, we? normally do IHCs with a variety of antibodies, depending on the? project.? My worries are that, in case buffered formalin could be? substituted, you can never be sure about IHC and maybe antibodies that? were working before stop doing it. Very many thanks for your thoughts and time in this issue. Greatly? appreciated! Cheers Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CMcgrad1 at hurleymc.com Fri Sep 30 10:52:39 2016 From: CMcgrad1 at hurleymc.com (Cynthia McGrady) Date: Fri, 30 Sep 2016 15:52:39 +0000 Subject: [Histonet] DAB neutralization and disposal Message-ID: <0053E7F9DD068946AC3541D0A35C098A0206E1D72B@Exchangemb1b.hmc.hurleymc.com> Gathering data to make a decision on how labs neutralize and dispose of DAB waste. Any input would be greatly appreciated.. Thanks..Cindy From patpxs at gmail.com Fri Sep 30 11:31:52 2016 From: patpxs at gmail.com (P Sicurello) Date: Fri, 30 Sep 2016 09:31:52 -0700 Subject: [Histonet] Histology Supervisor Position at UC San Diego Health Message-ID: Happy Friday Listers, We have an opening for a Histology supervisor. This person will supervise a staff of 13 (and growing) in Histology, IHC and EM. We would like: 1. At east 3 years of experience as a supervisor in a high volume academic setting. 2. HT/HTL certified 3. Experience with all aspects of histology, IHC and EM. EM is preferred but not mandatory. 4. Experience with LIS and tracking systems helpful. *Please go to ucsd.jobs.edu and look for #JMC83529 Histotechnologist Supervisor* A little information about us: Anatomic Pathology is growing rapidly. We will be moving into a new hospital (Jacobs Medical Center) soon. That will add another 11 ORs, making a total of 21. When the new Outpatient Pavilion opens there will be 8 more. We have about 40 staff, 20 pathologists and at least 16 residents. We will be hiring more staff as the volume increases. Histology has 3 VIP6, 1 VIP5, and 1 Peloris for processing. H&E and special stains (Ventana Special Stainer) are automated as is coverslipping. IHC has 3 Ventana Ultras. EM is getting a new microscope - an FEI Tecnai Spirit G2, and a new ultramicrotome. We perform routine histology, IF, muscle enzyme histochemistry, IHC (currently offering over 150 antibodies - with more on the horizon), and electron microscopy. Of course San Diego is the best city in the USA. Currently it is about 75 degrees and sunny. Please apply online. Feel free to contact me if you have any questions. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From carl.hobbs at kcl.ac.uk Fri Sep 30 12:46:08 2016 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 30 Sep 2016 17:46:08 +0000 Subject: [Histonet] DAB neutralization and disposal Message-ID: Store it and give it to your Chemical people ( sensible) or....follow John Kiernan's most excellent advice that he garnered ( in Histonet archives) I wonder what people do, who use polymer DAB kits? I reckon they chuck it down the sink.....do the Suppliers of such kits advice appropriate disposal? Curiously Carl