From twheelock at mclean.harvard.edu Mon Oct 3 08:55:32 2016 From: twheelock at mclean.harvard.edu (Wheelock, Timothy R.) Date: Mon, 3 Oct 2016 13:55:32 +0000 Subject: [Histonet] Immunohistochemistry Message-ID: <69718C0B0B3C414D9F8E7214AD400CC99FC70F7B@PHSX10MB11.partners.org> Hi Everyone: What is the designation of someone who is certified (registered) in immunohistochemistry? Thanks, Tim Wheelock Harvard Brain Bank Mclean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From mward at wakehealth.edu Mon Oct 3 09:05:15 2016 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Mon, 3 Oct 2016 14:05:15 +0000 Subject: [Histonet] Looking for a maintenance form Message-ID: Does anyone have a specific form that they use to indicate that a particular instrument is functioning as expected after service repairs or PMs? We need to document this information and I would like a simple form to fill out and include with the service reports. Just trying to not have to reinvent the wheel and would like to see what others are doing. Thanks in advance for your help. Martha Ward Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157 336-716-2109 From Ronald.Houston at nationwidechildrens.org Mon Oct 3 12:34:27 2016 From: Ronald.Houston at nationwidechildrens.org (Houston, Ronald) Date: Mon, 3 Oct 2016 17:34:27 +0000 Subject: [Histonet] Process Improvement and Quality Initiatives Message-ID: <10b3d6762ebb44908735c2ed45c9786b@l1perdwmbx02.childrensroot.net> We are in the process of re-examining what we discuss in our daily huddles with a view to involve all pathologists, and was wondering what others are doing in relation to topics for daily study and as part of a daily management system e.g. Metrics and Goals; Daily Readiness Assessment -MESA (Methods, Equipment, Supplies, Associates); Problem Management - ownership, expectations, countermeasures. Do pathologists get actively involved in huddles in your facilities? Any suggestions for getting all staff fully engaged? Thanks for any help/suggestions Ronnie Ronnie Houston, MS HT(ASCP)QIHC FIBMS Anatomic Pathology Manager Laboratory Services 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston at nationwidechildrens.org www.NationwideChildrens.org "Without continual growth and progress, such words as improvement, achievement, and success have no meaning." ~ Ben Franklin From tbraud at holyredeemer.com Mon Oct 3 13:02:00 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 3 Oct 2016 18:02:00 +0000 Subject: [Histonet] Histonet Digest, Vol 155, Issue 2 In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F808E7EE@HRHEX02-HOS.holyredeemer.local> I just instruct the service personnel to include a single statement on the service report that the instrument has been tested and is functioning properly following service. Worked for the last inspection. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 2. Looking for a maintenance form (Martha Ward-Pathology) ------------------------------ Message: 2 Date: Mon, 3 Oct 2016 14:05:15 +0000 From: Martha Ward-Pathology Subject: [Histonet] Looking for a maintenance form Does anyone have a specific form that they use to indicate that a particular instrument is functioning as expected after service repairs or PMs? We need to document this information and I would like a simple form to fill out and include with the service reports. Just trying to not have to reinvent the wheel and would like to see what others are doing. Thanks in advance for your help. Martha Ward Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157 336-716-2109 From rhoobarb23 at comcast.net Mon Oct 3 13:09:27 2016 From: rhoobarb23 at comcast.net (Ruben Carter) Date: Mon, 3 Oct 2016 13:09:27 -0500 Subject: [Histonet] Histologist seeking position in San Diego Message-ID: <9A25FFAD-5E89-4063-BC34-55EAAFEC7081@comcast.net> Hey histo world. I?m looking for histology work in the SoCal San Diego area (previous resident). I am a career histologist with 16 years of progressive experience in all aspect of histological studies. My employment includes clinical diagnostics, drug discovery, and research and development. I excel in all facets of histology (embedding, microtomy, cryotomy, manual and automated staining of IHC, molecular and special staining, image analysis including algorithm development and hardware setup). If anyone has any leads feel free to contact me. Thanks. rhoobarb23 at comcast.net From bhartologist at gmail.com Mon Oct 3 13:19:17 2016 From: bhartologist at gmail.com (Bharti Parihar) Date: Mon, 3 Oct 2016 11:19:17 -0700 Subject: [Histonet] Kaiser Permanente Berkeley Is Hiring!!!!!!!!!! Message-ID: Hello All! If any licensed HTs are interested in working for Kaiser Permanente in the Berkeley, CA area, please reply to this posting. Two evening shifts are being circulated within the department currently but if no one applies internally, it will go external. The shifts are 6:00 p.m. to 2:30 a.m. & 10:00 p.m. to 6:30 a.m. Kaiser has the best benefits an employer can provide! Cheers! -- Bharti Parihar, HT (ASCP)CM From narasimj at ohsu.edu Mon Oct 3 14:33:46 2016 From: narasimj at ohsu.edu (Jayasri Narasimhan) Date: Mon, 3 Oct 2016 19:33:46 +0000 Subject: [Histonet] Processor Troubleshooting Message-ID: Hi everyone, For the past two months in the research lab I work in, we've been dealing with poorly processed mouse livers. The following is the protocol, which is optimized for mouse mammary tissue, but we've been using it for all tissues. Fixation: ~48 hours in 10% nbf, shaking. 70% ethanol until processing; it sits in there anywhere from a couple of days to weeks. Processing (Sakura Tissue Tek VIP - 5) 70% ethanol 5 min 80% 30 min 95% - 1 30 min 95% - 2 40 min 100% -1 30 min 100% -2 30 min 100%-2 30 min Xylene-1 45 min Xylene-2 40 min Xylene-3 30 min Paraffin1 40 min Paraffin2 45 min Paraffin3 45 min The worst livers expanded on the ice bath, and had the hepatocytes appeared to have perinuclear cytoplasmic clearing. We ran PAS and glycogen doesn't fully account for their appearance. We've pm'ed our processor, compared runs on our processor to runs from the research histocore, and processed a few cassettes in surgical pathology using their protocol. By far, livers processed in Surg Path were the best. Their protocol starts with NBF and then goes into 80% alchohol. From then, each cycle is 40-60 minutes, so a bit longer than ours. Finally, the Sakura tech ran a warm water flush. The specimens run after this flush look similar to the ones run at the research histocore, so the processing problems seem to be resolved. My main question: what could the warm flush have done to resolve our processing issues? Since we're storing our tissues in 70% before putting them on the processor, aren't we preventing salt precipitation that could affect processing? Does anyone know the chemistry/science behind what might have happened here? Thanks, JN OHSU Lab From blayjorge at gmail.com Mon Oct 3 15:02:08 2016 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Mon, 3 Oct 2016 16:02:08 -0400 Subject: [Histonet] Looking for a paper Message-ID: Looking for a paper Dear Colleagues: Two major library searches by two different institutions and years of looking by a very competent librarian, still unable to find the reference below: Cunha, A. and W. E. Kerr. 1957. A genetical theory to explain sex-determination by arrhenotocous parthenogenesis.Forma et functio 1(4):___-___. Does anyone have this reference? More broadly, does this reference exist? If anyone has a constructive suggestion, please email it to me directly, blayjorge at gmail.com Gratefully, Jorge P.S. Apologies for potentially duplicate emails. Jorge A. Santiago-Blay, PhD http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ On Mon, Oct 3, 2016 at 3:33 PM, Jayasri Narasimhan via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi everyone, > > For the past two months in the research lab I work in, we've been dealing > with poorly processed mouse livers. The following is the protocol, which is > optimized for mouse mammary tissue, but we've been using it for all tissues. > > Fixation: > ~48 hours in 10% nbf, shaking. > 70% ethanol until processing; it sits in there anywhere from a couple of > days to weeks. > > Processing (Sakura Tissue Tek VIP - 5) > 70% ethanol 5 min > 80% 30 min > 95% - 1 30 min > 95% - 2 40 min > 100% -1 30 min > 100% -2 30 min > 100%-2 30 min > Xylene-1 45 min > Xylene-2 40 min > Xylene-3 30 min > Paraffin1 40 min > Paraffin2 45 min > Paraffin3 45 min > > The worst livers expanded on the ice bath, and had the hepatocytes > appeared to have perinuclear cytoplasmic clearing. We ran PAS and glycogen > doesn't fully account for their appearance. > > We've pm'ed our processor, compared runs on our processor to runs from the > research histocore, and processed a few cassettes in surgical pathology > using their protocol. > > By far, livers processed in Surg Path were the best. Their protocol starts > with NBF and then goes into 80% alchohol. From then, each cycle is 40-60 > minutes, so a bit longer than ours. > > Finally, the Sakura tech ran a warm water flush. The specimens run after > this flush look similar to the ones run at the research histocore, so the > processing problems seem to be resolved. > > My main question: what could the warm flush have done to resolve our > processing issues? Since we're storing our tissues in 70% before putting > them on the processor, aren't we preventing salt precipitation that could > affect processing? Does anyone know the chemistry/science behind what might > have happened here? > > Thanks, > JN > OHSU Lab > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From bpaniccia.5 at gmail.com Mon Oct 3 15:18:01 2016 From: bpaniccia.5 at gmail.com (Bernard Paniccia) Date: Mon, 3 Oct 2016 16:18:01 -0400 Subject: [Histonet] Need filing cabinets Message-ID: Hello there histonet. I am looking to acquire some Tissue-Tek cassette filing cabinets. They're the standard 6- drawer 15" x 9.5" x 17" plastic stackables. We're located in central Ohio, but we could truck out somewhere to pick up a sufficiently large quantity of them if necessary. If anyone is interested, please contact me. Thank you! Bernard Bernard S. Paniccia, HTL(ASCP)CM Phylogeny Inc. 1476 Manning Parkway Powell, OH 43065 Phone: (614) 846-6161 | Fax: (877) 633-6442 bernard at phylogenyinc.com From twheelock at mclean.harvard.edu Mon Oct 3 15:34:04 2016 From: twheelock at mclean.harvard.edu (Wheelock, Timothy R.) Date: Mon, 3 Oct 2016 20:34:04 +0000 Subject: [Histonet] Salary range Message-ID: <69718C0B0B3C414D9F8E7214AD400CC99FC71085@PHSX10MB11.partners.org> Hi Everyone: First thank you for letting me know the designation of someone who is certified in immunohistochemistry. I have a second question. Do you have an idea of the average salary range, in the Boston area, for someone who starts a job managing a pathology or neuropathology laboratory (by managing, I mean being responsible for all the functions of the laboratory, while working alone), if they are a new employee, have an HT/HTL and QIHC certification, and have 3-4 years of experience in a pathology or neuropathology laboratory? Thanks so much, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From diane.satterfield at duke.edu Mon Oct 3 15:47:07 2016 From: diane.satterfield at duke.edu (Diane Satterfield) Date: Mon, 3 Oct 2016 20:47:07 +0000 Subject: [Histonet] Leica TP1020 Processor question Message-ID: I have acquired a Leica TP1020 tissue processor for my lab. I have never had to process tissue before so I am reaching out for help. I have no idea what protocol I need to be using. I am working with human brain tumor tissue that has been fixed in 10%NBF for 24-48 hours then transferred to 70% EtOH. I need to know what to do with it from here. Can someone help me with this please? Thanks. Diane L. Satterfield, BS Manager Brain Tumor BioRepository Research Program Leader Duke University Medical Center Brain Tumor Center Biorepository and Database diane.satterfield at duke.edu office 919-684-4642 pager 919-970-7328 fax 919-684-4975 CONFIDENTIALITY NOTICE: The information contained in this electronic mail is sensitive, protected information intended only for the addressee(s). Any other person, including anyone who believes he/she might have received it due to an addressing error, is requested to notify the sender immediately by return electronic mail, and to delete it without further reading or retention. The information is not to be forwarded to or shared unless in compliance with Duke Medicine policies on confidentiality and/or with the approval of the sender. From jqb7 at cdc.gov Tue Oct 4 11:45:16 2016 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue, 4 Oct 2016 16:45:16 +0000 Subject: [Histonet] bins Message-ID: Hi everyone, Does anyone have a source for clear plastic bins that will accommodate the metal processing trays for the VIP-6 tissue processor? Would like something for the grossing station. Thanks, Jeanine H. Sanders, BS, HT (ASCP), QIHC Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS/G-32 Atlanta, GA 30329 jqb7 at cdc.gov 404-639-3590 From jvickroy at SpringfieldClinic.com Tue Oct 4 12:31:25 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Tue, 4 Oct 2016 17:31:25 +0000 Subject: [Histonet] Handling Breast Lumpectomy Specimens with radioactive seed localization Message-ID: <9B1A1501A800064397369BD8072E6BCA065D33C3@E2K10DB.springfieldclinic.com> Our organization is looking into the workflow necessary to handle breast lumpectomies with radioactive seed localization. Right now we have more questions than answers. I have looked at several articles and am aware of the drastic changes that will need to be in place to handle the radioactive seed. My experience in the past has been handling a wire-located lumpectomy proceeded usually by a sentinel node biopsy by frozen section. From what I read the sentinel node biopsy procedure will probably not be changed, however I have several questions regarding handling the lumpectomy specimen and would appreciate any thoughts from someone already handling these specimens. I know there are precautions on how to handle and remove the seed as well as procedures in case the seed is cut during the removal. I am trying to figure out how the workflow will proceed. Wire-located lumpectomy procedure: Currently the sentinel nodes are sent to the lab from surgery and frozen sections are performed. The pathologist then calls the surgeon and depending on whether the sentinel nodes were positive further nodal dissections may or may not be necessary. The wire-located lumpectomy specimen is usually sent over to pathology after the sentinel node biopsies. The lumpectomy specimen has usually been x-rayed to show the location of the wire prior to being received by pathology. Pathology then usually gets a copy of the x-ray along with the fresh lumpectomy specimen. Next the pathologist or pathology assistant would place the specimen in formalin and let it fix for sometimes overnight. The next day the fixed lumpectomy specimen was grossed and sectioned for histologic examination. Special care is taken to take specific sections where the wire was implanted. Radioactive seed lumpectomy procedure questions: 1. Since the lumpectomy is obviously "hot" what precautions are taken to remove the seed and give it back to radiology? 2. Does the pathologist or pathology assistant remove the seed? 3. After seed removal is the specimen handled similarly to a wire-located specimen.....In other words is the specimen then fixed for a period of time before grossing? 4. How is the location of the seed marked in the lumpectomy specimen when the seed is removed? Do the pathologists use an ink to mark the location, do they then fix the tissue as before? Finally another "WRENCH" to this new procedure is that we usually send our larger tissue specimens to be grossed and processed at a hospital lab. We are being asked to perform the frozen section onsite and then send the fixed tissue left over from the frozen sections with the lumpectomy specimen to the hospital lab. Obviously the radioactive seed has to be removed before transportation to the hospital lab. As you can see there are more questions than answers and I would appreciate hearing from someone that is already handling the new radioactive seep lumpectomy specimens. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From Stefanie.Harris at crl.com Tue Oct 4 14:27:57 2016 From: Stefanie.Harris at crl.com (Harris, Stefanie) Date: Tue, 4 Oct 2016 19:27:57 +0000 Subject: [Histonet] Slide dryer broke, suggestions needed Message-ID: Hello all, We have been using a Mopec RotoDry as our slide dryer for over 10 years but it recently started to have issues with being able heat and maintain the required temperature. I have a quote for the TBS Shur/Dry II but it seems significantly smaller than what we currently have. The Shur/Dry III seems like it would be too much for us. What do you use as a dryer? Thank you in advance for any suggestions, Stefanie Stefanie Harris Research Assistant, Histology Research Animal Diagnostic Services | Charles River From bchick2g at hotmail.com Tue Oct 4 14:40:26 2016 From: bchick2g at hotmail.com (Amanda Miele) Date: Tue, 4 Oct 2016 19:40:26 +0000 Subject: [Histonet] (no subject) Message-ID: Aass?x1 Amanda Miele, HT (ASCP) From bbarnard at personifysearch.com Tue Oct 4 15:23:01 2016 From: bbarnard at personifysearch.com (Blake Barnard) Date: Tue, 4 Oct 2016 16:23:01 -0400 Subject: [Histonet] Career Opportunity - Advanced Field Support Specialist Message-ID: <44f78533d5f05523528158a66146fe24@mail.gmail.com> Hello Histonet, Our exclusively retained client has opened a new Advanced Field Applications/Support Specialist opportunity and I wanted to reach out regarding a few specifics and see if anyone would be interested in learning more. Our client is a global leader in medical devices and diagnostics and they are looking for a field based applications/support role. The ideal candidate will have a strong background and understanding of Immunohistochemistry (IHC) and histology. This individual will provide technical training on use of systems and must be comfortable presenting to customers at all levels. The company is part of a $9 billion organization and is growing. This position is a full-time, direct-hire role with competitive salary and full benefits. If you or anyone you know may be interested in learning more about this position, please contact me directly at *bbarnard at personifysearch.com * Thank you! *Blake Barnard* Talent Management Executive [image: https://docs.google.com/a/personifysearch.com/uc?id=0ByHopg_78n39ZklCMWpubm40VDQ&export=download] *Personify * 416 S. Dawson St. Raleigh, NC 27601 (Direct) 252-299-5876 www.personifysearch.com [image: https://docs.google.com/a/personifysearch.com/uc?id=0ByHopg_78n39QWJEcGwtanJSM0E&export=download] [image: https://docs.google.com/a/personifysearch.com/uc?id=0ByHopg_78n39YThBR2pfd1czVEk&export=download] [image: https://docs.google.com/a/personifysearch.com/uc?id=0ByHopg_78n39WFhrYjIyak84ZE0&export=download] [image: https://docs.google.com/a/personifysearch.com/uc?id=0ByHopg_78n39RmQ4NndwWFNteGs&export=download] From Blanca.Lopez at UTSouthwestern.edu Wed Oct 5 09:06:15 2016 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Wed, 5 Oct 2016 14:06:15 +0000 Subject: [Histonet] Cutting blocks for mouse tissue troubleshooting Message-ID: Hello Histonets! I need to find out a better way to cut the whole pancreas mouse tissue without wrinkles, Does anybody has suggestion. I am having trouble to obtain a nice section without wrinkles. It is pretty big piece and crawl in on my embedding, is hard to stretch out without breaking. I cannot use ammonia water because they use the unstained for research and I don't want get discrepancies in any other stains. Suggestions??? thanks Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From jriggleman at globusmedical.com Wed Oct 5 10:34:05 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Wed, 5 Oct 2016 15:34:05 +0000 Subject: [Histonet] Knife Sharpening Service Message-ID: Can anyone recommend a good Knife Sharpening service around the east coast? We are located in the PA area. We have tried CL Sturkey and Dorn & Hart Microedge. It doesn't necessarily need to be East Coast.. We are just looking for ~1 Week turn around time. Thank you, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From srishan at mail.holyname.org Wed Oct 5 11:08:11 2016 From: srishan at mail.holyname.org (srishan at mail.holyname.org) Date: Wed, 5 Oct 2016 12:08:11 -0400 Subject: [Histonet] mats Message-ID: What is everyone using as floor mats in histogy. The mats we have are very thick and with Holes, we are finding it difficult to move the chairs on them to get close to the microtome for cutting. We were told that carpet of any kind cannot be use. If we remove the mats, the floors are very slippery. Any idea will be greatly appreciated. Mala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From jvickroy at SpringfieldClinic.com Wed Oct 5 11:27:44 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Wed, 5 Oct 2016 16:27:44 +0000 Subject: [Histonet] Kappa and Lambda ISH Message-ID: <9B1A1501A800064397369BD8072E6BCA065D3F69@E2K10DB.springfieldclinic.com> Our department does not perform IHC stains nor ISH Kappa and Lambda stains. Therefore we do not perform the CAP proficiency testing. The pathologists participate in PIP testing and do not read ER, PR, or Her2 (predictive markers). The pathologists do however interpret IHC stains and an occasional Kappa and Lambda stain. Are there any other requirements that the pathologist or department needs to fulfil? Again the patholgosits here are interpreting the IHC stains and reading Kappa and Lambda (the only ISH stains they interpret). Also when I did work at an institution that performed Kappa and Lambda there was no CAP PT survey for Kappa and Lambda so we had to come up with an alternate PT assessment method. Does CAP now have a Kappa and Lambda PT survey? Your input is appreciated. Thanks Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From ADuddey at firsthealth.org Wed Oct 5 11:32:07 2016 From: ADuddey at firsthealth.org (Duddey, Aimee) Date: Wed, 5 Oct 2016 16:32:07 +0000 Subject: [Histonet] mats In-Reply-To: References: Message-ID: <09FBA01CA9B6374A83C5C76E09E46188A34B12B6@EXMAIL1-FHC.firsthealth.org> If you google "laboratory sticky floor mat" you will see images of the mats that we used until we renovated. They keep paraffin off of your feet and the wheels on your chairs. Simply peel off the top layer to clean up During renovation we installed a rubber sheet flooring throughout the entire lab that eliminated the need for anti fatigue mats as well as being slip resistant. This flooring has been wonderful. Aimee M. Duddey, MLT(ASCP) Assistant Director of Laboratory - Pathology FH Moore Regional Hospital 910-715-5286 office 910715-1944 fax -----Original Message----- From: Nirmala Srishan via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, October 05, 2016 12:08 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] mats What is everyone using as floor mats in histogy. The mats we have are very thick and with Holes, we are finding it difficult to move the chairs on them to get close to the microtome for cutting. We were told that carpet of any kind cannot be use. If we remove the mats, the floors are very slippery. Any idea will be greatly appreciated. Mala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robert.jacox at thermofisher.com Wed Oct 5 12:41:08 2016 From: robert.jacox at thermofisher.com (Jacox, Robert A.) Date: Wed, 5 Oct 2016 17:41:08 +0000 Subject: [Histonet] Knife Sharpening Service In-Reply-To: References: Message-ID: Try DDK (Delaware Diamond Knives), it has been some time since I used them but they were always a good choice Sent from my iPad > On Oct 5, 2016, at 5:43 PM, Jessica Riggleman via Histonet wrote: > > Can anyone recommend a good Knife Sharpening service around the east coast? We are located in the PA area. We have tried CL Sturkey and Dorn & Hart Microedge. > > It doesn't necessarily need to be East Coast.. We are just looking for ~1 Week turn around time. > > Thank you, > Jessica > > > _____________________________________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > > Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock at mclean.harvard.edu Wed Oct 5 13:25:40 2016 From: twheelock at mclean.harvard.edu (Wheelock, Timothy R.) Date: Wed, 5 Oct 2016 18:25:40 +0000 Subject: [Histonet] mats In-Reply-To: References: Message-ID: <69718C0B0B3C414D9F8E7214AD400CC99FC7240F@PHSX10MB11.partners.org> Hi Mala: We use the following: PIP Technical Contamination Control Mat Vendor: Fisher Catalogue #: 19-122-822 They work very well; just peel off the top layer, and you have a fresh one ready for the next round of sectioining. Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA -----Original Message----- From: Nirmala Srishan via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, October 05, 2016 12:08 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] mats What is everyone using as floor mats in histogy. The mats we have are very thick and with Holes, we are finding it difficult to move the chairs on them to get close to the microtome for cutting. We were told that carpet of any kind cannot be use. If we remove the mats, the floors are very slippery. Any idea will be greatly appreciated. Mala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From rsrichmond at gmail.com Wed Oct 5 13:47:45 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Wed, 5 Oct 2016 14:47:45 -0400 Subject: [Histonet] Handling Breast Lumpectomy Specimens with radioactive seed localization Message-ID: Jim Vickroy, Histology Manager at Springfield [Illinois] Clinic asks: "Our organization is looking into the workflow necessary to handle breast lumpectomies with radioactive seed localization (RSL)". Thanks for the heads-up - as usual, pathologists and histologists don't get told. Nursing practice is to assume that what we do is ritual disposal of corpse poison, and we're immune to all hazards. And "seed" is a magickal word that shuts off thinking. Apparently RSL has been around for more than ten years. A titanium "seed" containing about 20 microcuries of radioactive iodine 125 is placed in the patient's breast lesion a day or so before surgery, usually eliminating the need for wire localization or specimen radiography. The "seed" is not removed from the specimen, which is delivered to the pathology department. Iodine 125 is a gamma emitter with a half-life of about 60 days (unlike the technetium 99m used in the sentinel node biopsy, which has a half-life of only six hours). It's a considerably more hazardous material, though I think it's safe to briefly handle the "seed" with gloved hands. What you don't want to do is lose the thing in your clothing or in the laboratory, since it will take nearly two years to decay to a safe level of radioactivity. According to my online resource, the "seed" is recovered from the pathology department with elaborate ceremony, in the presence of a "radiation safety officer". I've yet to encounter a hospital that had a radiation safety officer, and often there's nobody who knows the difference between radiation and radioactive material. If this procedure is thrown at you without warning, I suggest that your pathologist stage a carefully planned hissy-fit. I found an excellent online resource for this subject. Read the section about laboratory procedures. http://www.teambest.com/besttotalsolutions/PDFs/RadioactiveSeedLocalizationI125_LTDauer_et_al_Oct2013.pdf From wrglo5 at aol.com Wed Oct 5 13:49:52 2016 From: wrglo5 at aol.com (Lori Wright) Date: Wed, 5 Oct 2016 14:49:52 -0400 Subject: [Histonet] Faxitron Message-ID: <3049D15C-B38C-4975-8D61-A3C9DE3C819F@aol.com> Hi All, I was wondering if anyone was using a Faxitron in their Lab? Thanks, Lori Wright Lead Technician Surgical Pathology Duke Medicine Sent from my iPhone From LNormington at uwhealth.org Wed Oct 5 14:35:17 2016 From: LNormington at uwhealth.org (Normington Lacy) Date: Wed, 5 Oct 2016 19:35:17 +0000 Subject: [Histonet] Handling Breast Lumpectomy Specimens with radioactive seed localization In-Reply-To: <9B1A1501A800064397369BD8072E6BCA065D33C3@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA065D33C3@E2K10DB.springfieldclinic.com> Message-ID: <7F7174244DDD1A49BFBEE8A845BC2F2B23B50D@UWHC-MBX05.uwhis.hosp.wisc.edu> James, Luckly our breast surgeons are good at notifying our Pathologists' Assistants when a case with radioactive seeds is sent to our laboratory. Everything under the sun is labeled with radiation stickers, and additional stickers are provided with the tissue requisition. We also have a chain of custody sheet that is delivered with the specimen. 1. Since the lumpectomy is obviously "hot" what precautions are taken to remove the seed and give it back to radiology? The individual removing the seed wears a radiation detection ring. The ring is evaluated monthly by our radiation safety department. The specimen is scanned with a survey meter to located all seeds. Once the seed is removed, the seeds are placed in a lead lined PIG and stored in a locked safe within our gross room. A representative from our breast center is required to pick up the seeds within 24 hours and document this on the chain of custody form. 2. Does the pathologist or pathology assistant remove the seed? The Pathologists' Assistants performs the removal as they are required to perform CBT on radioactive safety training.. 3. After seed removal is the specimen handled similarly to a wire-located specimen.....In other words is the specimen then fixed for a period of time before grossing? Yes, normal fixation procedure is followed. Histology staff are notified of the case, however, no special radiation safety precautions are needed. 4. How is the location of the seed marked in the lumpectomy specimen when the seed is removed? Do the pathologists use an ink to mark the location, do they then fix the tissue as before? We follow an inking protocol for lumpectomies, whether wire or seeds. If you are interested in our procedure, please let me know and I can email it to you. Lacy Normington, HTL(ASCP)CM Manager, Surgical Pathology Lab Services UW Health - UW Hospital 600 Highland Avenue Madison, WI 53792-2472 Phone: 608-890-9373 -----Original Message----- From: Vickroy, James via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, October 04, 2016 12:31 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Handling Breast Lumpectomy Specimens with radioactive seed localization Our organization is looking into the workflow necessary to handle breast lumpectomies with radioactive seed localization. Right now we have more questions than answers. I have looked at several articles and am aware of the drastic changes that will need to be in place to handle the radioactive seed. My experience in the past has been handling a wire-located lumpectomy proceeded usually by a sentinel node biopsy by frozen section. From what I read the sentinel node biopsy procedure will probably not be changed, however I have several questions regarding handling the lumpectomy specimen and would appreciate any thoughts from someone already handling these specimens. I know there are precautions on how to handle and remove the seed as well as procedures in case the seed is cut during the removal. I am trying to figure out how the workflow will proceed. Wire-located lumpectomy procedure: Currently the sentinel nodes are sent to the lab from surgery and frozen sections are performed. The pathologist then calls the surgeon and depending on whether the sentinel nodes were positive further nodal dissections may or may not be necessary. The wire-located lumpectomy specimen is usually sent over to pathology after the sentinel node biopsies. The lumpectomy specimen has usually been x-rayed to show the location of the wire prior to being received by pathology. Pathology then usually gets a copy of the x-ray along with the fresh lumpectomy specimen. Next the pathologist or pathology assistant would place the specimen in formalin and let it fix for sometimes overnight. The next day the fixed lumpectomy specimen was grossed and sectioned for histologic examination. Special care is taken to take specific sections where the wire was implanted. Radioactive seed lumpectomy procedure questions: 1. Since the lumpectomy is obviously "hot" what precautions are taken to remove the seed and give it back to radiology? 2. Does the pathologist or pathology assistant remove the seed? 3. After seed removal is the specimen handled similarly to a wire-located specimen.....In other words is the specimen then fixed for a period of time before grossing? 4. How is the location of the seed marked in the lumpectomy specimen when the seed is removed? Do the pathologists use an ink to mark the location, do they then fix the tissue as before? Finally another "WRENCH" to this new procedure is that we usually send our larger tissue specimens to be grossed and processed at a hospital lab. We are being asked to perform the frozen section onsite and then send the fixed tissue left over from the frozen sections with the lumpectomy specimen to the hospital lab. Obviously the radioactive seed has to be removed before transportation to the hospital lab. As you can see there are more questions than answers and I would appreciate hearing from someone that is already handling the new radioactive seep lumpectomy specimens. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LNormington at uwhealth.org Wed Oct 5 14:39:34 2016 From: LNormington at uwhealth.org (Normington Lacy) Date: Wed, 5 Oct 2016 19:39:34 +0000 Subject: [Histonet] Faxitron In-Reply-To: <3049D15C-B38C-4975-8D61-A3C9DE3C819F@aol.com> References: <3049D15C-B38C-4975-8D61-A3C9DE3C819F@aol.com> Message-ID: <7F7174244DDD1A49BFBEE8A845BC2F2B23B529@UWHC-MBX05.uwhis.hosp.wisc.edu> We use a Faxitron in our grossing room for breast and prostate specimens. We have also used it for calcium chases of a tissue block. Do you have any specific questions? Lacy Normington, HTL(ASCP)CM Manager, Surgical Pathology Lab Services UW Health - UW Hospital 600 Highland Avenue Madison, WI 53792-2472 Phone: 608-890-9373 -----Original Message----- From: Lori Wright via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, October 05, 2016 1:50 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Faxitron Hi All, I was wondering if anyone was using a Faxitron in their Lab? Thanks, Lori Wright Lead Technician Surgical Pathology Duke Medicine Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jvickroy at SpringfieldClinic.com Wed Oct 5 15:48:05 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Wed, 5 Oct 2016 20:48:05 +0000 Subject: [Histonet] Performing PAS with Diastase by Hand Message-ID: <9B1A1501A800064397369BD8072E6BCA065D46D4@E2K10DB.springfieldclinic.com> We are starting to perform a PAS with diastase for fungus to be used by our dermatopathologist. At first we weren't sure he was going to need a method that used diastase but he now prefers it. Problem is our PAS counter kit did not come with diastase. Please don't tell me to use saliva although I'm old enough to know we did for years. We did order some powered diastase that was quite expensive and of course the working solution had a very short shelf-life. Can anybody recommend a product and vendor that is relatively inexpensive and economical to use? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From CIngles at uwhealth.org Wed Oct 5 17:06:54 2016 From: CIngles at uwhealth.org (Ingles Claire) Date: Wed, 5 Oct 2016 22:06:54 +0000 Subject: [Histonet] Performing PAS with Diastase by Hand In-Reply-To: <9B1A1501A800064397369BD8072E6BCA065D46D4@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA065D46D4@E2K10DB.springfieldclinic.com> Message-ID: We get our Diastase powder from American Mastertech. Claire Dermatopathology lab University of Wisconsin Hospital and Clinics ________________________________________ From: Vickroy, James via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, October 05, 2016 3:48 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Performing PAS with Diastase by Hand We are starting to perform a PAS with diastase for fungus to be used by our dermatopathologist. At first we weren't sure he was going to need a method that used diastase but he now prefers it. Problem is our PAS counter kit did not come with diastase. Please don't tell me to use saliva although I'm old enough to know we did for years. We did order some powered diastase that was quite expensive and of course the working solution had a very short shelf-life. Can anybody recommend a product and vendor that is relatively inexpensive and economical to use? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun at hhchealth.org Wed Oct 5 18:35:43 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Wed, 5 Oct 2016 23:35:43 +0000 Subject: [Histonet] Kappa and Lambda ISH In-Reply-To: <9B1A1501A800064397369BD8072E6BCA065D3F69@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA065D3F69@E2K10DB.springfieldclinic.com> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E9539B47B@HHCEXCHMB03.hhcsystem.org> Yes, the CAP now has a PT survey for HPV, EBV, and Kappa/Lambda in situ hybridization (ISH). Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Vickroy, James via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, October 05, 2016 12:28 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Kappa and Lambda ISH Our department does not perform IHC stains nor ISH Kappa and Lambda stains. Therefore we do not perform the CAP proficiency testing. The pathologists participate in PIP testing and do not read ER, PR, or Her2 (predictive markers). The pathologists do however interpret IHC stains and an occasional Kappa and Lambda stain. Are there any other requirements that the pathologist or department needs to fulfil? Again the patholgosits here are interpreting the IHC stains and reading Kappa and Lambda (the only ISH stains they interpret). Also when I did work at an institution that performed Kappa and Lambda there was no CAP PT survey for Kappa and Lambda so we had to come up with an alternate PT assessment method. Does CAP now have a Kappa and Lambda PT survey? Your input is appreciated. Thanks Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From pablo.sanchez at usc.es Thu Oct 6 04:10:31 2016 From: pablo.sanchez at usc.es (=?UTF-8?Q?Pablo_S=c3=a1nchez_Quinteiro?=) Date: Thu, 6 Oct 2016 11:10:31 +0200 Subject: [Histonet] KU Size In-Reply-To: References: Message-ID: Dear Histonetters, Searching for a product -an enzyme- its size it is not indicated in volume or weight. It is labelled as 25 KU. Does it mean that the vial contains 25.000 activity units? Thanks Pablo From LRaff at uropartners.com Thu Oct 6 08:05:24 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 6 Oct 2016 13:05:24 +0000 Subject: [Histonet] Lab related blog post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10FF5860@COLOEXCH01.uropartners.local> Hoping all are doing well. Good lab-bad lab blog. http://www.chicagonow.com/downsize-maybe/2016/10/ben-stiller-and-psa-fly-elizabeth-holmes-and-theranos-die/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From Erin.Martin at ucsf.edu Thu Oct 6 08:31:25 2016 From: Erin.Martin at ucsf.edu (Martin, Erin) Date: Thu, 6 Oct 2016 13:31:25 +0000 Subject: [Histonet] Vacuolated and torn? Message-ID: <24B7B291CC88D04AB663958E77A1F59DC78AE1@ex09.net.ucsf.edu> Good morning everyone! One of my pathologists says that we are having a problem with the tissue on the slides looking vacuolated and torn. He is convinced it is from microtomy. Anyone have any ideas? I was thinking that it might be a processing issue. Thanks in advance! Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. From tmcampbe at fmh.org Thu Oct 6 09:35:50 2016 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Thu, 6 Oct 2016 14:35:50 +0000 Subject: [Histonet] Clia Competency Form Message-ID: Does anyone have a CLIA competency form for histology supervisor, histotechs, and technical supervisors that they would be willing to share? It would be greatly appreciated!!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 From Tyanna.Stuckey at northsoundderm.com Thu Oct 6 09:55:29 2016 From: Tyanna.Stuckey at northsoundderm.com (Tyanna Stuckey) Date: Thu, 6 Oct 2016 14:55:29 +0000 Subject: [Histonet] Small Auto cover slipper to use with Histoclear. Message-ID: Hello Histonet! I work in a lab that uses Histoclear as our clearing agent on our automatic stainer. I am looking for an automatic cover slipper/cover slipping tape that we can use with this. The thing about our lab is that it is very small and we have limited space. If there is not something out there for us, would it be possible to use xylene on only the cover slipper to avoid changing our protocols? Thanks! Tyanna Stuckey Histology Technician North Sound Dermatology tyanna.stuckey at northsoundderm.com From liz at premierlab.com Thu Oct 6 10:29:31 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Thu, 6 Oct 2016 09:29:31 -0600 Subject: [Histonet] Cutting blocks for mouse tissue troubleshooting In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02ED70DDFB75@SBS2K8.premierlab.local> Blanca If your samples are fixed and processed properly the sections should cut without wrinkles. We process mouse pancreas on a 30 minute cycle - 30 minutes per station starting at 50% alcohol. We trim and soak blocks on ice and we normally do not have issue with wrinkles. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, October 05, 2016 8:06 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cutting blocks for mouse tissue troubleshooting Hello Histonets! I need to find out a better way to cut the whole pancreas mouse tissue without wrinkles, Does anybody has suggestion. I am having trouble to obtain a nice section without wrinkles. It is pretty big piece and crawl in on my embedding, is hard to stretch out without breaking. I cannot use ammonia water because they use the unstained for research and I don't want get discrepancies in any other stains. Suggestions??? thanks Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jriggleman at globusmedical.com Thu Oct 6 12:12:46 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Thu, 6 Oct 2016 17:12:46 +0000 Subject: [Histonet] Faxitron In-Reply-To: <3049D15C-B38C-4975-8D61-A3C9DE3C819F@aol.com> References: <3049D15C-B38C-4975-8D61-A3C9DE3C819F@aol.com> Message-ID: Hi, We use a Faxitron machine in our lab. _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. -----Original Message----- From: Lori Wright [mailto:wrglo5 at aol.com] Sent: Wednesday, October 05, 2016 2:50 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Faxitron Hi All, I was wondering if anyone was using a Faxitron in their Lab? Thanks, Lori Wright Lead Technician Surgical Pathology Duke Medicine Sent from my iPhone From rsrichmond at gmail.com Thu Oct 6 12:48:39 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 6 Oct 2016 13:48:39 -0400 Subject: [Histonet] Handling Breast Lumpectomy Specimens with radioactive seed localization Message-ID: Further researching the topic, I've learned that there are two recently introduced non-radioactive alternatives, Savi Scout and Sentimag. I don't understand how they work. Coincidentally, I received a circular in this morning's mail with information about a continuing medical education event on breast disease, with presentations about both of these non-radioactive techniques. Bob Richmond Samurai Pathologist Maryville TN From Tyanna.Stuckey at northsoundderm.com Thu Oct 6 14:51:10 2016 From: Tyanna.Stuckey at northsoundderm.com (Tyanna Stuckey) Date: Thu, 6 Oct 2016 19:51:10 +0000 Subject: [Histonet] Sakura/Tissue Tek Glas Coverslipper Message-ID: Hi all, I'm curious if anyone uses a Sakura/Tissue Tek Glas Coverslipper in their lab, and if they use xylene/xylene based media or if it would be possible to use a xylene substitute product on the machine? Thanks Tyanna Stuckey Histology Technician North Sound Dermatology tyanna.stuckey at northsoundderm.com From Richard.Cartun at hhchealth.org Thu Oct 6 17:43:44 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Thu, 6 Oct 2016 22:43:44 +0000 Subject: [Histonet] Vacuolated and torn? In-Reply-To: <24B7B291CC88D04AB663958E77A1F59DC78AE1@ex09.net.ucsf.edu> References: <24B7B291CC88D04AB663958E77A1F59DC78AE1@ex09.net.ucsf.edu> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E9539BB68@HHCEXCHMB03.hhcsystem.org> Microtomy is so important, but often over-looked. You might try giving the paraffin block(s) to different people and have them all cut H&Es, and then have your pathologist compare them. If everyone's sections are suboptimal, then the problem is with fixation and/or processing. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Martin, Erin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, October 06, 2016 9:31 AM To: histonet Subject: [Histonet] Vacuolated and torn? Good morning everyone! One of my pathologists says that we are having a problem with the tissue on the slides looking vacuolated and torn. He is convinced it is from microtomy. Anyone have any ideas? I was thinking that it might be a processing issue. Thanks in advance! Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From mills at 3scan.com Thu Oct 6 18:39:07 2016 From: mills at 3scan.com (Caroline Miller) Date: Thu, 6 Oct 2016 16:39:07 -0700 Subject: [Histonet] Vacuolated and torn? In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E9539BB68@HHCEXCHMB03.hhcsystem.org> References: <24B7B291CC88D04AB663958E77A1F59DC78AE1@ex09.net.ucsf.edu> <9215BD4B0BA1B44D962A71C758B68D2E9539BB68@HHCEXCHMB03.hhcsystem.org> Message-ID: If it is a block issue you can also often see 'white' bits on the block surface that correlate to the torn areas on the slide, try 'polishing' the block at 1um until they go away, cool and then try sectioning again. I am unsure how that relates to vacuolation - as I have usually seen that referred to an intracellular event - and not to refer to a regular hole. I also suggest having someone else cut a block and then compare. yours, mills On Thu, Oct 6, 2016 at 3:43 PM, Cartun, Richard via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Microtomy is so important, but often over-looked. You might try giving > the paraffin block(s) to different people and have them all cut H&Es, and > then have your pathologist compare them. If everyone's sections are > suboptimal, then the problem is with fixation and/or processing. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & > Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > > -----Original Message----- > From: Martin, Erin via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, October 06, 2016 9:31 AM > To: histonet > Subject: [Histonet] Vacuolated and torn? > > Good morning everyone! > > One of my pathologists says that we are having a problem with the tissue > on the slides looking vacuolated and torn. He is convinced it is from > microtomy. Anyone have any ideas? I was thinking that it might be a > processing issue. > > > > Thanks in advance! > > Erin Martin, Histology Supervisor > UCSF Dermatopathology and Oral Pathology Service > 1701 Divisadero St, San Francisco, CA 94044 > 415-353-7248 > > Confidentiality Notice > The information transmitted is intended only for the person or entity to > which it is addressed and may contain confidential and/or privileged > material. Any review, retransmission, dissemination or other use of, or > taking of any action in reliance upon, this information by persons or > entities other than the intended recipient is prohibited. If you receive > this in error please contact the sender and delete the material from any > computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From monica.fining at macaulay.cuny.edu Thu Oct 6 20:28:05 2016 From: monica.fining at macaulay.cuny.edu (Monica Fining) Date: Thu, 6 Oct 2016 21:28:05 -0400 Subject: [Histonet] Technovit 9100 PMMA Depolymerization Message-ID: Hello, any information on how to depolymerize bone samples that are in Technovit 9100 Poly(methyl methacrylate) PMMA polymer? From jriggleman at globusmedical.com Fri Oct 7 09:37:15 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Fri, 7 Oct 2016 14:37:15 +0000 Subject: [Histonet] Our Lab Needs a Microtome - Suggestions Message-ID: Hello, I am looking for a similar machine to the Reichert-Jung Polycut S Microtome. I am looking to cut MMA thin sections on rabbit femoral condyles (somewhat larger than usual specimen size). If anyone can recommend a newer microtome or is looking to sell, that would be great. I am unaware of the new stuff out there.. Thank you, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From BAMoe at gundersenhealth.org Fri Oct 7 15:46:16 2016 From: BAMoe at gundersenhealth.org (Moe, Barbi A) Date: Fri, 7 Oct 2016 20:46:16 +0000 Subject: [Histonet] Performing PAS with Diastase by Hand In-Reply-To: References: <9B1A1501A800064397369BD8072E6BCA065D46D4@E2K10DB.springfieldclinic.com>, Message-ID: <8B4B31D17067E540AC760B4A30190B9901B61E85D2@LXEXMB01.gundluth.org> Jim - We use amylase from porcine pancreas, from Sigma. Barb Moe Gundersen Health System La Crosse WI ________________________________________ From: Ingles Claire via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, October 05, 2016 5:06 PM To: Vickroy, James; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Performing PAS with Diastase by Hand We get our Diastase powder from American Mastertech. Claire Dermatopathology lab University of Wisconsin Hospital and Clinics ________________________________________ From: Vickroy, James via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, October 05, 2016 3:48 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Performing PAS with Diastase by Hand We are starting to perform a PAS with diastase for fungus to be used by our dermatopathologist. At first we weren't sure he was going to need a method that used diastase but he now prefers it. Problem is our PAS counter kit did not come with diastase. Please don't tell me to use saliva although I'm old enough to know we did for years. We did order some powered diastase that was quite expensive and of course the working solution had a very short shelf-life. Can anybody recommend a product and vendor that is relatively inexpensive and economical to use? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sprice2003 at gmail.com Fri Oct 7 17:12:00 2016 From: sprice2003 at gmail.com (Sally Price) Date: Fri, 7 Oct 2016 18:12:00 -0400 Subject: [Histonet] South Carolina Society for Histotechnology's Fall Expo Message-ID: Greetings HistoNetters - In the event that you couldn't make one of the state-society meetings that were held in the Spring or the NSH Symposium/Convention just a few weeks ago, I hope you'll consider attending the South Carolina Society for Histotechnology's Fall Expo, which will be held Friday, November 4th through Sunday, November 6th at the Litchfield Beach and Golf Resort in Pawley's Island - which is right between Myrtle Beach and Charleston. Our meeting will feature nationally recognized speakers including Liz Chlipala, B.S., HTL(ASCP), former South Carolinian Joe Myers, M.S., CT(ASCP)QIHC, and Bill DeSalvo, HT(ASCP) - who are not only knowledgeable in their respective fields, but also quite entertaining...or so I'm told. For more information on this event, please contact the SCSH's President, Chad McMahan at: < clmcmah at clemson.edu > Hope to see ya'll there! -- Sally Price From mwerdler at gmail.com Mon Oct 10 12:01:13 2016 From: mwerdler at gmail.com (Mca Werdler) Date: Mon, 10 Oct 2016 12:01:13 -0500 Subject: [Histonet] Black gold Message-ID: Good day everyone, I was wondering if anyone has any experience with black gold, for staining myelin in brain sections, and is willing to share this protocol. Thank you Maarten From Richard.Cartun at hhchealth.org Mon Oct 10 14:04:29 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 10 Oct 2016 19:04:29 +0000 Subject: [Histonet] Formalin in Physicians' Offices Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E9539CEC5@HHCEXCHMB03.hhcsystem.org> Do physicians' offices need to have procedures/policies "on-site" regarding formalin if they put patient biopsy specimens into the small formalin containers? What about formalin spill kits? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From mdpraet at gmail.com Mon Oct 10 20:12:00 2016 From: mdpraet at gmail.com (Mequita Praet) Date: Mon, 10 Oct 2016 21:12:00 -0400 Subject: [Histonet] Formalin in Physicians' Offices Message-ID: <80pkllel4af1ds257u3x3gm9.1476148320986@email.android.com> Yes, that would be the best. We even included formaldehyde safety training for the nurses/medical assistants. Contact dermatitis can develop even from small amounts. Mequita Praet, HTL(ASCP)SLS Former Manager Dermatology Associates Boca Raton, FL "Cartun, Richard via Histonet" wrote: >Do physicians' offices need to have procedures/policies "on-site" regarding formalin if they put patient biopsy specimens into the small formalin containers? What about formalin spill kits? Thank you. > >Richard > >Richard W. Cartun, MS, PhD >Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory >Director, Biospecimen Collection Programs >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 972-1596 >(860) 545-2204 Fax > > >This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks at gmail.com Tue Oct 11 09:33:21 2016 From: amosbrooks at gmail.com (Amos Brooks) Date: Tue, 11 Oct 2016 10:33:21 -0400 Subject: [Histonet] Tunel with Apoptag Message-ID: Hi, I am contentedly using the Millipore (Chemicon) Apoptag IHC kit for Tunel stains. It works great... *BUT* I have been getting requests for a fluorescent tag rather than a chromogenic. The contents of the fluorescent kits are identical except for the anti-Dig. In the chromogenic kit is HRP tagged and the fluorescent is IF tagged. All I need is the IF tagged part, but wouldn't you know it, they don't sell just that part. No, you need to buy the whole dang kit for the one component you need. So, clearly I need to find an alternative fluorescent tagged anti-DIG antibody that would detect this TDT enzyme. Any suggestions? Thanks, Amos From abadesuyi at nrh-ok.com Tue Oct 11 10:00:59 2016 From: abadesuyi at nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Tue, 11 Oct 2016 10:00:59 -0500 Subject: [Histonet] Billings for ER, PR and HER-2 Message-ID: <04EE4F75BB5FB246ADB68D69B7460443B1006B6197@MAIL.nrhnt.nrh-ok.com> Hi, We are having billing issue with ER, PR and HER-2.Our business supervisor was asking whether we should bill them together or separately. I will greatly appreciate your contributions Thanks, Banjo ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From tbraud at holyredeemer.com Tue Oct 11 12:28:44 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 11 Oct 2016 17:28:44 +0000 Subject: [Histonet] Formalin in physician offices Message-ID: <48E053DDF6CE074DB6A7414BA05403F808FA70@HRHEX02-HOS.holyredeemer.local> We provide formalin training and small volume spill kits and neutralizing wipes for all physician offices owned by our health care system. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Today's Topics: 2. Formalin in Physicians' Offices (Cartun, Richard) 3. Re: Formalin in Physicians' Offices (Mequita Praet) ---------------------------------------------------------------------- Message: 2 Date: Mon, 10 Oct 2016 19:04:29 +0000 From: "Cartun, Richard" Subject: [Histonet] Formalin in Physicians' Offices Do physicians' offices need to have procedures/policies "on-site" regarding formalin if they put patient biopsy specimens into the small formalin containers? What about formalin spill kits? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax ------------------------------ Message: 3 Date: Mon, 10 Oct 2016 21:12:00 -0400 From: Mequita Praet Subject: Re: [Histonet] Formalin in Physicians' Offices Yes, that would be the best. We even included formaldehyde safety training for the nurses/medical assistants. Contact dermatitis can develop even from small amounts. Mequita Praet, HTL(ASCP)SLS Former Manager Dermatology Associates Boca Raton, FL From abuchiane at bmhvt.org Tue Oct 11 12:41:49 2016 From: abuchiane at bmhvt.org (Anita Buchiane) Date: Tue, 11 Oct 2016 17:41:49 +0000 Subject: [Histonet] Labeling System Message-ID: <4034E71604330C4B8E10D1538DFB2455ECC9CA6E@BMHEXCH02.bmhvt.org> Hi Histonetters, Curious to know how other labs label specimens. For example this is how we do it: First specimen container in a case is labeled "A" and the first block is "1" so if we had 5 blocks on the specimen they would be labeled A1 thru A5 Subsequent specimen containers in the same case are labeled "B", "C", etc so B1 thru B5 , C1 thru C5, etc _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ From IMYLES at PARTNERS.ORG Tue Oct 11 13:17:53 2016 From: IMYLES at PARTNERS.ORG (Myles, Irene) Date: Tue, 11 Oct 2016 18:17:53 +0000 Subject: [Histonet] How to process crystals Message-ID: <32DFC0C34EFB774B822FDBB5434AAFBC01198A3F4C@PHSX10MB20.partners.org> Can someone tell me the best way to process urate crystals. Thank you for your help Irene Myles, HT (ASCP) Senior Histology Technician Brigham and Women's Hospital Histology; Dept. of Pathology Amory Bldg; 3rd flr; RM 368J 75 Francis Street Boston, MA 02215 617-732-5454 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From alicia.ortega at colorado.edu Tue Oct 11 18:47:01 2016 From: alicia.ortega at colorado.edu (Alicia Marie Ortega) Date: Tue, 11 Oct 2016 23:47:01 +0000 Subject: [Histonet] Villanueva Osteochrome Bone Stain on already embedded ground sections Message-ID: Hello everyone, I was just wondering if anyone has a protocol for staining undecalcified bone already embedded in poly(methyl methacylate) using the Villanueva Osteochrome Bone Stain? I have 30-40 micron thick samples and I cannot deplasticize my samples as suggested by the manufacturer's protocol because my samples have been glued-to and ground-on plastic slides. Thanks in advance for your help! Sincerely, Alicia Ortega Postdoctoral Research Associate University of Colorado, Boulder From GauchV at mail.amc.edu Wed Oct 12 08:18:47 2016 From: GauchV at mail.amc.edu (Gauch, Vicki) Date: Wed, 12 Oct 2016 13:18:47 +0000 Subject: [Histonet] Billing Questions Message-ID: Hi, I've been asked to post a question regarding Pathology technical billing. With all of the changes that have been instituted over the past few years on the technical billing side in Pathology and the fact that our computer system cannot automate it for us we have been manually billing our IHC,etc. . This is becoming a real problem since the supervisor and myself are the ones doing this and we are also working on the bench along with other duties. We are wondering who does that billing in your lab? Do you have biller/coders ? Is it technical staff ? If it is technical staff, what level ? Has anyone been able to get that billing automated? Any info would be greatly appreciated. Thank you, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From victor_tobias at comcast.net Wed Oct 12 08:34:50 2016 From: victor_tobias at comcast.net (victor_tobias at comcast.net) Date: Wed, 12 Oct 2016 06:34:50 -0700 Subject: [Histonet] Billing Questions In-Reply-To: References: Message-ID: Vicki, My first question is what computer/LIS system are you using? Are you a private lab or part of a hospital? We are in an academic medical center, have our own Pathology coders, but all billing is done by the hospital. We generate the information for the technical and pro charges and send a daily file. We have a team of 3 that run the LIS for Pathology. We use PowerPath and straight out of the box, it doesn?t do billing well. A lot of customization over the years and ongoing to keep up with changes. Victor UWMC Seattle, WA Sent from Mail for Windows 10 From: Gauch, Vicki via Histonet Sent: Wednesday, October 12, 2016 6:19 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Billing Questions Hi, I've been asked to post a question regarding Pathology technical billing. With all of the changes that have been instituted over the past few years on the technical billing side in Pathology and the fact that our computer system cannot automate it for us we have been manually billing our IHC,etc. . This is becoming a real problem since the supervisor and myself are the ones doing this and we are also working on the bench along with other duties. We are wondering who does that billing in your lab? Do you have biller/coders ? Is it technical staff ? If it is technical staff, what level ? Has anyone been able to get that billing automated? Any info would be greatly appreciated. Thank you, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun at hhchealth.org Wed Oct 12 13:35:29 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Wed, 12 Oct 2016 18:35:29 +0000 Subject: [Histonet] Billing Questions In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E9539E1C4@HHCEXCHMB03.hhcsystem.org> I can only speak to IHC billing. My staff (now 2 people) in our IHC Lab does all the technical IHC billing manually. We average about 3,500 billable IHC tests per month now. The volume used to be higher, but our healthcare system sold most of our outreach business. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Gauch, Vicki via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, October 12, 2016 9:19 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Billing Questions Hi, I've been asked to post a question regarding Pathology technical billing. With all of the changes that have been instituted over the past few years on the technical billing side in Pathology and the fact that our computer system cannot automate it for us we have been manually billing our IHC,etc. . This is becoming a real problem since the supervisor and myself are the ones doing this and we are also working on the bench along with other duties. We are wondering who does that billing in your lab? Do you have biller/coders ? Is it technical staff ? If it is technical staff, what level ? Has anyone been able to get that billing automated? Any info would be greatly appreciated. Thank you, Vicki Gauch AMCH Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From CMcgrad1 at hurleymc.com Thu Oct 13 06:27:31 2016 From: CMcgrad1 at hurleymc.com (Cynthia McGrady) Date: Thu, 13 Oct 2016 11:27:31 +0000 Subject: [Histonet] Chart Message-ID: <0053E7F9DD068946AC3541D0A35C098A0206E1DA71@Exchangemb1b.hmc.hurleymc.com> Does anyone have a chart or log they use for staining correlations for Immuno machines? We are correlating our Bond Max and Bond 3 machines. Thanks. Cindy From mtighe at trudeauinstitute.org Thu Oct 13 08:17:25 2016 From: mtighe at trudeauinstitute.org (Mike Tighe) Date: Thu, 13 Oct 2016 13:17:25 +0000 Subject: [Histonet] Human placental tissue Message-ID: Wondering if anyone has any tips for processing human placental tissue before I try to reinvent the wheel! (formalin fixed tissue to paraffin) Thanks for any help! Mike From lblazek at digestivespecialists.com Thu Oct 13 11:26:20 2016 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Thu, 13 Oct 2016 12:26:20 -0400 Subject: [Histonet] new lab Message-ID: <5A2BD13465E061429D6455C8D6B40E3917781EC612@IBMB7Exchange.digestivespecialists.com> Hi all, It's finally official. We will be building a new facility with ground breaking in the spring! I have seen the drawings for the designated area in the new building for the histology lab. Now I could use any suggestions that you all may have in designing a new lab. Thanks in advance! Linda Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com From jqb7 at cdc.gov Thu Oct 13 11:49:20 2016 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Thu, 13 Oct 2016 16:49:20 +0000 Subject: [Histonet] new lab In-Reply-To: <5A2BD13465E061429D6455C8D6B40E3917781EC612@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E3917781EC612@IBMB7Exchange.digestivespecialists.com> Message-ID: <27de469a622b470584b634116a659236@cdc.gov> Make it twice as big as you think you need! Jeanine H. Sanders Infectious Diseases Pathology Branch Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS-G32 Atlanta, GA 30329 -----Original Message----- From: Blazek, Linda via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, October 13, 2016 12:26 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] new lab Hi all, It's finally official. We will be building a new facility with ground breaking in the spring! I have seen the drawings for the designated area in the new building for the histology lab. Now I could use any suggestions that you all may have in designing a new lab. Thanks in advance! Linda Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From EzanyaObeten at KentuckyOneHealth.org Thu Oct 13 12:04:12 2016 From: EzanyaObeten at KentuckyOneHealth.org (Obeten, Ezanya) Date: Thu, 13 Oct 2016 17:04:12 +0000 Subject: [Histonet] new lab In-Reply-To: <27de469a622b470584b634116a659236@cdc.gov> References: <5A2BD13465E061429D6455C8D6B40E3917781EC612@IBMB7Exchange.digestivespecialists.com>, <27de469a622b470584b634116a659236@cdc.gov> Message-ID: Windows boost Morale in my opinion ________________________________________ From: Sanders, Jeanine (CDC/OID/NCEZID) via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, October 13, 2016 12:49 PM To: Blazek, Linda; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] new lab CAUTION: This email is not from a CHI source. Only click links or open attachments you know are safe. ...................................................................... Make it twice as big as you think you need! Jeanine H. Sanders Infectious Diseases Pathology Branch Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS-G32 Atlanta, GA 30329 -----Original Message----- From: Blazek, Linda via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, October 13, 2016 12:26 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] new lab Hi all, It's finally official. We will be building a new facility with ground breaking in the spring! I have seen the drawings for the designated area in the new building for the histology lab. Now I could use any suggestions that you all may have in designing a new lab. Thanks in advance! Linda Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=_A40u2SgX1cncjo6korkK9A3cTBPwn4RaDoed0JOtgrS_4vc942o-Za7k0DbbFv1&m=zeWDxLdaT0O1cUy1-naQ1WK42s359vRFjYqK3i_cozw&s=kPPJJcuDcYae7Cow7JoJ2vq7Sz6Y2xC438W7WGp3WeM&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DQICAg&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=_A40u2SgX1cncjo6korkK9A3cTBPwn4RaDoed0JOtgrS_4vc942o-Za7k0DbbFv1&m=zeWDxLdaT0O1cUy1-naQ1WK42s359vRFjYqK3i_cozw&s=kPPJJcuDcYae7Cow7JoJ2vq7Sz6Y2xC438W7WGp3WeM&e= This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From rjbuesa at yahoo.com Thu Oct 13 12:06:27 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 13 Oct 2016 17:06:27 +0000 (UTC) Subject: [Histonet] new lab In-Reply-To: <5A2BD13465E061429D6455C8D6B40E3917781EC612@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E3917781EC612@IBMB7Exchange.digestivespecialists.com> Message-ID: <824117088.333607.1476378387861@mail.yahoo.com> If you already saw the drawings for the designated area, is it not too late now to make changes?If you think you can give input, my only suggestion is that you set your working areas in a way that they follow the workflow and ideally should be close to the surgery suits.Ren? On Thursday, October 13, 2016 12:47 PM, "Blazek, Linda via Histonet" wrote: Hi all, It's finally official.? We will be building a new facility with ground breaking in the spring!? I have seen the drawings for the designated area in the new building for the histology lab.? Now I could use any suggestions that you all may have in designing a new lab. Thanks in advance! Linda Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From djemge11 at gmail.com Thu Oct 13 14:17:59 2016 From: djemge11 at gmail.com (Donna Emge) Date: Thu, 13 Oct 2016 15:17:59 -0400 Subject: [Histonet] Laboratory Developed Test LDT process example for antibodies, specifically P16 Message-ID: P16 antibody is soon to move from an FDA approved IVD to a LDT. I have not performed a LDT before. I read COM.4020. Would someone be willing to share one of their LDT workups for an antibody that meets CAP requirements? I want to make sure I am on the right track and do not miss anything. *Donna J. Emge, HT(ASCP)* Histology Manager South Bend Medical Foundation (574) 234-4176 ext. 1345 DEmge at sbmf.org Djemge11 at gmail.com From Timothy.Morken at ucsf.edu Fri Oct 14 11:32:56 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 14 Oct 2016 16:32:56 +0000 Subject: [Histonet] Laboratory Developed Test LDT process example for antibodies, specifically P16 In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF6FDA2D89@ex07.net.ucsf.edu> Do you mean the list required by COM.40200, or a validation procedure? The list is just a list of those test you have modified from manufacturer's instructions, or developed in-house from ASR's (any IHC test you do in which you modify dilutions, detection, AR etc). For validation, The FDA LDT rule is not final yet (at least was not at NSH in September when we had a workshop on the topic). I suppose CAP could require additional validation beyond what CLIA already outlines (and is outlined in the COM items after 40200). CAP currently has a recommendations for ER, PR and Her2: in the range of 25-50 negative, 25-50 positive with a range of positive from weak to strong. Statistically concordant compared to known antibody results. If FDA goes through with their proposal you can expect much more extensive validation (vendors run hundreds to thousands of cases to meet FDA validation requirements). -----Original Message----- From: Donna Emge via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, October 13, 2016 12:18 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Laboratory Developed Test LDT process example for antibodies, specifically P16 P16 antibody is soon to move from an FDA approved IVD to a LDT. I have not performed a LDT before. I read COM.4020. Would someone be willing to share one of their LDT workups for an antibody that meets CAP requirements? I want to make sure I am on the right track and do not miss anything. *Donna J. Emge, HT(ASCP)* Histology Manager South Bend Medical Foundation (574) 234-4176 ext. 1345 DEmge at sbmf.org Djemge11 at gmail.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From areichard at lmhealth.org Fri Oct 14 13:43:52 2016 From: areichard at lmhealth.org (Amanda Reichard) Date: Fri, 14 Oct 2016 18:43:52 +0000 Subject: [Histonet] EBUS/EUS Pathology Billing Message-ID: <873e96ffcc8f43da9e624acb696a4e6c@LMH-EXMB-1.lmhealth.org> Happy Friday Everyone! I have a question for those of you who send a tech or a cytotechnologist to assist the pathologist with EBUS/EUS procedures... What CPT codes are you using when billing? Are you billing a technical component for the staining of the slides during the procedure? (We use Diff Quik) Do you just bill the professional component? Thanks for any help with this! Amanda Reichard, HTL (ASCP)cm Histology/Cytology Supervisor Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (220) 564-4163 areichard at lmhealth.org From rjbuesa at yahoo.com Fri Oct 14 15:12:48 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 14 Oct 2016 20:12:48 +0000 (UTC) Subject: [Histonet] Fw: Dr. Matsionis In-Reply-To: <901924598.1332738.1476467899790@mail.yahoo.com> References: <901924598.1332738.1476467899790.ref@mail.yahoo.com> <901924598.1332738.1476467899790@mail.yahoo.com> Message-ID: <2027840728.627563.1476475968938@mail.yahoo.com> Hi colleagues:I have just received the sad news that the prestigious Russian histopathologist Prof. Alexander Matsionis passed away (see included message).Although his name is almost unknown in our?field he always was extremely enthusiast about new?histopathology procedures and helped introducing isopropanol?tissue processing in scores of Russian labs.My thoughts to his family, colleagues and friends.Ren? ? On Friday, October 14, 2016 1:58 PM, Maxim Peshkov wrote: Dear Rene!An 12 October was died our great friend, pathologist, doctor Alexander Matsionis Academician of RANS (Russian Academy of Natural Sciences), MD, PhD, proffesor and teacher. He fought with acute monocityc leikemia. He was only 59 years old.?He will stay in our memory as a man who made a great contribution to the Russian and pathological anatomy as a science (haematology, urology, breast pathology, soft tissue and endocrine pathology and many others) and as a profession. He organized Rostov pathological-anatomical bureau. He also united almost all the pathologists of southern Russia's regions and gave way in the life of so many young pathologists, who have already proved themselves excellent specialists. He helped to development of our processing method with isopropanol and mineral oil. He was a great friend and cheerful, always helped the needy both as pathologists, as patients also.He always appreciated your help and support. In our memory and hearts he will stay forever. Maxim. From eddessa at emory.edu Fri Oct 14 15:48:20 2016 From: eddessa at emory.edu (Dessasau III, Evan) Date: Fri, 14 Oct 2016 20:48:20 +0000 Subject: [Histonet] Fw: Dr. Matsionis In-Reply-To: <2027840728.627563.1476475968938@mail.yahoo.com> References: <901924598.1332738.1476467899790.ref@mail.yahoo.com> <901924598.1332738.1476467899790@mail.yahoo.com>, <2027840728.627563.1476475968938@mail.yahoo.com> Message-ID: thank you for letting us know Rene. It is always sad to lose good friend and good professionals E-van ________________________________ From: Rene J Buesa via Histonet Sent: Friday, October 14, 2016 4:12:48 PM To: Histonet Cc: Maxim Peshkov Subject: [Histonet] Fw: Dr. Matsionis Hi colleagues:I have just received the sad news that the prestigious Russian histopathologist Prof. Alexander Matsionis passed away (see included message).Although his name is almost unknown in our field he always was extremely enthusiast about new histopathology procedures and helped introducing isopropanol tissue processing in scores of Russian labs.My thoughts to his family, colleagues and friends.Ren? On Friday, October 14, 2016 1:58 PM, Maxim Peshkov wrote: Dear Rene!An 12 October was died our great friend, pathologist, doctor Alexander Matsionis Academician of RANS (Russian Academy of Natural Sciences), MD, PhD, proffesor and teacher. He fought with acute monocityc leikemia. He was only 59 years old. He will stay in our memory as a man who made a great contribution to the Russian and pathological anatomy as a science (haematology, urology, breast pathology, soft tissue and endocrine pathology and many others) and as a profession. He organized Rostov pathological-anatomical bureau. He also united almost all the pathologists of southern Russia's regions and gave way in the life of so many young pathologists, who have already proved themselves excellent specialists. He helped to development of our processing method with isopropanol and mineral oil. He was a great friend and cheerful, always helped the needy both as pathologists, as patients also.He always appreciated your help and support. In our memory and hearts he will stay forever. Maxim. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From ashnews at comcast.net Sun Oct 16 08:52:06 2016 From: ashnews at comcast.net (ashnews at comcast.net) Date: Sun, 16 Oct 2016 13:52:06 +0000 (UTC) Subject: [Histonet] Removing Tissue From Tape In-Reply-To: <990232086.21207709.1476625773774.JavaMail.zimbra@comcast.net> Message-ID: <1271759611.21209610.1476625926155.JavaMail.zimbra@comcast.net> Good Morning, ? Although the laboratory stopped using tape years ago we are still facing issues with "OLD" tape slide being requested for review or moving them to storage only?to discover the tape has come off and the tissue is stuck to it.? I hesitate to just re-coverslip it to the slide by hand.? Can anyone give some ideas of how to best preserve these slides that were once taped? ? Thank You, Pam Marcum ? From rjbuesa at yahoo.com Sun Oct 16 10:42:14 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Sun, 16 Oct 2016 15:42:14 +0000 (UTC) Subject: [Histonet] Removing Tissue From Tape In-Reply-To: <1271759611.21209610.1476625926155.JavaMail.zimbra@comcast.net> References: <990232086.21207709.1476625773774.JavaMail.zimbra@comcast.net> <1271759611.21209610.1476625926155.JavaMail.zimbra@comcast.net> Message-ID: <491503242.705006.1476632534923@mail.yahoo.com> I think your best option is to manually re-coverslip to the slide.Ren? On Sunday, October 16, 2016 10:00 AM, Pamela Marcum via Histonet wrote: Good Morning, ? Although the laboratory stopped using tape years ago we are still facing issues with "OLD" tape slide being requested for review or moving them to storage only?to discover the tape has come off and the tissue is stuck to it.? I hesitate to just re-coverslip it to the slide by hand.? Can anyone give some ideas of how to best preserve these slides that were once taped? ? Thank You, Pam Marcum ? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.benavides at eae.csic.es Sun Oct 16 12:57:41 2016 From: j.benavides at eae.csic.es (Julio Benavides) Date: Sun, 16 Oct 2016 19:57:41 +0200 Subject: [Histonet] Substituting formalin by 70%ethanol in fixed samples. In-Reply-To: <491503242.705006.1476632534923@mail.yahoo.com> References: <990232086.21207709.1476625773774.JavaMail.zimbra@comcast.net> <1271759611.21209610.1476625926155.JavaMail.zimbra@comcast.net> <491503242.705006.1476632534923@mail.yahoo.com> Message-ID: <20161016195741.Horde.U5VqLayFDk-Xu0badh8TDA1@webmail.csic.es> Hi there, This is me again with formalin issues. The health and safety officer of my institute keeps on in her crusade of eliminating formalin from the world. After your helpful emails, I convinced her that you do need formalin to fix samples, other approach would need to much setup IHC protocols. Then and with good intention at heart, she has suggested that, one possible solution to reduce formalin presence in the institute could be, once samples have been fixed in buffered formalin (10%) and embedded in wax, to substitute it by 70% ethanol until the sample is discharged. Has anybody tried anything similar? What do you think? Would it be possible to come back to formalin in case of necessity (let?s say we want to retrim fixed samples after they have been in ethanol 70% for 5 months? would you trust them for IHC? HE?). Thanks again for your help Julio From owensk2 at ccf.org Mon Oct 17 06:48:14 2016 From: owensk2 at ccf.org (Owens, Katrina) Date: Mon, 17 Oct 2016 11:48:14 +0000 Subject: [Histonet] Help with CryoJane Bone cutting Message-ID: <51E813F67A862847A3DEA0781684F8919C410B@CC-CLEXMB58.cc.ad.cchs.net> Hello All: I recently acquired a CryoJane unit but it doesn't seem to work in regards to bone cutting. It works just fine for soft tissue, but getting the bone to transfer from the tape to the slide just isn't happening. I have tried, successfully, using other UV sources to transfer the bone from the tape to the slides, but just not with the CryoJane. Any help or advice in this matter would be greatly appreciated. Thank you and have a good day! Katrina Owens owensk2 at ccf.org =================================== Please consider the environment before printing this e-mail Cleveland Clinic is ranked as one of the top hospitals in America by U.S.News & World Report (2015). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From mills at 3scan.com Mon Oct 17 11:23:36 2016 From: mills at 3scan.com (Caroline Miller) Date: Mon, 17 Oct 2016 09:23:36 -0700 Subject: [Histonet] Substituting formalin by 70%ethanol in fixed samples. In-Reply-To: <20161016195741.Horde.U5VqLayFDk-Xu0badh8TDA1@webmail.csic.es> References: <990232086.21207709.1476625773774.JavaMail.zimbra@comcast.net> <1271759611.21209610.1476625926155.JavaMail.zimbra@comcast.net> <491503242.705006.1476632534923@mail.yahoo.com> <20161016195741.Horde.U5VqLayFDk-Xu0badh8TDA1@webmail.csic.es> Message-ID: Hi Julio, I can speak to a research / core setting where I often advised my users to place their tissues in 70% ethanol following adequate formalin fixation to maintain epitopes for potential downstream IHC studies. I have found tissue can be placed in 70% and maintained indefinitely. They do not 'unfix'. Recently though I was speaking to a pathologist who doesn't like that protocol as she sees nuclear changes in tissues that have been in 70% for extended periods. So, yes, I think that idea is good in principle. I would say that the staff time taken, and extra reagent costs, of changing all those biopsies over into another solution, plus the additional chemical waste generated is not worth simply making sure the original formalin containers are well sealed after grossing and / or purchasing one of those specimen collection cupboards that filters the air coming through. I do understand the dangers of formalin, but considering how much of it is in use and how many of us on this list have been exposed to it for the majority of our lives and we are still OK! (well, maybe, I suppose that depends on your definition of OK is :) Leica seem to think this is OK too! http://www.leicabiosystems.com/pathologyleaders/fixation-and-fixatives-5-practical-procedures-to-optimise-quality-the-effects-of-heat-and-microwaves/ Ultimately you would have to perform studies to check the effects on your downstream processes and proof this procedure in your own lab. yours, mills On Sun, Oct 16, 2016 at 10:57 AM, Julio Benavides via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > Hi there, > > This is me again with formalin issues. The health and safety officer of my > institute keeps on in her crusade of eliminating formalin from the world. > After your helpful emails, I convinced her that you do need formalin to fix > samples, other approach would need to much setup IHC protocols. Then and > with good intention at heart, she has suggested that, one possible solution > to reduce formalin presence in the institute could be, once samples have > been fixed in buffered formalin (10%) and embedded in wax, to substitute it > by 70% ethanol until the sample is discharged. Has anybody tried anything > similar? What do you think? Would it be possible to come back to formalin > in case of necessity (let?s say we want to retrim fixed samples after they > have been in ethanol 70% for 5 months? would you trust them for IHC? HE?). > > Thanks again for your help > > Julio > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From lhamilton at wcplaboratories.com Mon Oct 17 14:22:26 2016 From: lhamilton at wcplaboratories.com (Lisa Hamilton) Date: Mon, 17 Oct 2016 14:22:26 -0500 Subject: [Histonet] Clip-On Magnifiers for Embedding Message-ID: <000901d228ab$cdacac90$690605b0$@com> In the past I have seen clip-on magnifiers that are used to embed tissue. Of course now that I need one I cannot remember what catalog I saw them in! I was hoping someone might know where I can find them. Thank You -Lisa From Lacie.Algeo at providence.org Mon Oct 17 14:53:28 2016 From: Lacie.Algeo at providence.org (Algeo, Lacie A) Date: Mon, 17 Oct 2016 19:53:28 +0000 Subject: [Histonet] Beta-Catenin Message-ID: <24C4B3C167E5694887AB594C7602CE3A03CE1CCE@WN35104.or.providence.org> Hi All, What are people using for Beta-catenin control tissue? Is anyone using tissue that is known specifically to have a mutation for the beta-catenin gene? If so, is this something that is purchased? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo at providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From N.A.M.Bax at tue.nl Tue Oct 18 07:26:43 2016 From: N.A.M.Bax at tue.nl (Bax, N.A.M.) Date: Tue, 18 Oct 2016 12:26:43 +0000 Subject: [Histonet] Picrosirius Red Message-ID: <67502D640FCB0641AACF2BE17717060C325A9D1D@xserver30b.campus.tue.nl> Dear Histosearch, I just became a member and I hope you can provide me with advice. I'm really curious about the effect of not dissolving Direct Red 80 in 1.3% saturated Picric Acid but in 0.9-1.1% alkalimetric Picric acid solution. The reason why I'm curious is that I want to perform a staining on paraffin slides for Sirius Red and in my current lab they have Picrosirius red dissolved in 0.9-1.1% alkalimetric Picric acid, but the samples were I want to compare them to are stained in Picrosirius red made with the standard protocol with saturated Picric Acid. I measured the pH of the Picrosirius Red made with 0.9-1.1% alkalimetric Picric acid and this is ~pH 2.0 and all the Direct Red is dissolved, so can I assume that the staining will be comparable to Picrosirius Red dissolved in saturated Picric acid? Thank you very much in advance. Best Noortje Noortje AM Bax, PhD | Laboratory for Cell and Tissue Engineering | Biomedical Engineering | Eindhoven University of Technology, P.O. Box 513, Groene Loper, Gebouw 15, Gemini-Zuid 4.108, 5600 MB Eindhoven, The Netherlands | T: +31-40-2474830 | F: +31 40-2473744 |E-mail: n.a.m.bax at tue.nl| From jriggleman at globusmedical.com Tue Oct 18 12:08:23 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Tue, 18 Oct 2016 17:08:23 +0000 Subject: [Histonet] Macrophage Stain Message-ID: Hello, Could you please help me find a macrophage stain that will work with sheep (specifically sheep laminectomy sections)? We are thinking RAM-11 or HAM-56, but unsure. Also, what about Rabbit PLF? Please note both are embedded in mega cassettes for paraffin processing. Thank you, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From liz at premierlab.com Tue Oct 18 12:27:26 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Tue, 18 Oct 2016 11:27:26 -0600 Subject: [Histonet] Macrophage Stain In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02ED87BEFAA5@SBS2K8.premierlab.local> Jessica We have not tried Ram-11 in sheep it's a macrophage antibody against rabbit, in our hands it did not cross react with human, cyno or rat. We use MAC387 to stain macrophages in sheep. It's not entirely specific to macrophages but it is what we have used in the past. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Jessica Riggleman via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, October 18, 2016 11:08 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Macrophage Stain Hello, Could you please help me find a macrophage stain that will work with sheep (specifically sheep laminectomy sections)? We are thinking RAM-11 or HAM-56, but unsure. Also, what about Rabbit PLF? Please note both are embedded in mega cassettes for paraffin processing. Thank you, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.benavides at eae.csic.es Tue Oct 18 12:41:57 2016 From: j.benavides at eae.csic.es (Julio Benavides) Date: Tue, 18 Oct 2016 19:41:57 +0200 Subject: [Histonet] Macrophage Stain In-Reply-To: References: Message-ID: <20161018194157.Horde._SfmVem4zbCJagl-0M_pJw1@webmail.csic.es> Hi Jessica, there are actually quite a number of Abs working on sheep tissues. Take a look at this paper, most of them work in sheep (I believe all but gamm-IFN). Let me know if you cannot access the paper and I will send you a copy. https://www.ncbi.nlm.nih.gov/pubmed/27315822 Happy to comment on the IHC conditions if you have any doubt. Cheers Julio Jessica Riggleman via Histonet escribi?: > Hello, > > Could you please help me find a macrophage stain that will work with > sheep (specifically sheep laminectomy sections)? We are thinking > RAM-11 or HAM-56, but unsure. > > Also, what about Rabbit PLF? > > Please note both are embedded in mega cassettes for paraffin processing. > > Thank you, > Jessica > > > > _____________________________________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > > Confidentiality Note:? This email is confidential and intended solely > for the use of the individual to whom it is addressed. If you are not > the intended recipient, be advised that you have received this email > in error and that any use, dissemination, forwarding, printing, or > copying of this email is strictly prohibited. If you have received > this email in error please contact the sender. Any views or opinions > presented are solely those of the author and do not necessarily > represent those of Globus Medical, Inc. Although this email and any > attachments are believed to be free of any virus or other defects > which might affect any computer or IT system into which they are > received, no responsibility is accepted by Globus Medical, Inc. for > any loss or damage arising in any way from the receipt or use thereof. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly at merck.com Tue Oct 18 14:10:49 2016 From: brett_connolly at merck.com (Connolly, Brett M) Date: Tue, 18 Oct 2016 15:10:49 -0400 Subject: [Histonet] Books and atlases Message-ID: Hi all, I am weeding out my library in preparation for retirement. If anyone is interested in any of these let me know, they are all in like new condition. I am not giving them away. I have more titles Short Protocols in Molecular Biology 4thEd; ISBN 0-471-32938-X Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology; ISBN 1-881299-43-0 Diagnostic Immunohistochemistry 2nd Ed; ISBN 0-443-06652-3 Atlas of Dermatopathology; ISBN 0-7216-4886-X Atlas of UroSurgical Anatomy; ISBN 0-7216-3955-0 Comparative Anatomy and Histology: A Mouse and Human atlas; ISBN 978-0-12-381361-9 Human Histology 2nd Ed; ISBN 0-7234-2485-3 A Color Atlas of Histology; ISBN 0-673-99190-3 Histology for Pathologists 2nd Ed; ISBN 0-397-51718-1 Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Kimberly at animalreferencepathology.com Wed Oct 19 05:31:57 2016 From: Kimberly at animalreferencepathology.com (Kimberly Marshall) Date: Wed, 19 Oct 2016 10:31:57 +0000 Subject: [Histonet] Large whole eye processing Message-ID: Good morning. I was hoping to get some input on processing and cutting large whole eyes ( horse, whale, large wild life). The study is just for diagnostic ocular pathology with the whole eye section the goal. Any input would be appreciated. Thanks Kimberly Kimberly Marshall H.T.(ASCP) Histology Lab Supervisor Toll Free 1-800-426-2099 Fax 801-584-5104 PO Box 17580 Salt Lake City, Utah 84107 www.animalreferencepathology.com Advancing the art and science of veterinary medicine From rford at hcmhcares.org Wed Oct 19 06:36:18 2016 From: rford at hcmhcares.org (Rhonda Ford) Date: Wed, 19 Oct 2016 07:36:18 -0400 Subject: [Histonet] Helicobacter pylori control blocks Message-ID: Does anyone have any extra Helicobacter pylori control blocks? Thanks in advance. -- Rhonda Ford, Histology Lab Henry Community Health Main Campus 1000 North 16th Street New Castle, Indiana 47362 (765) 521-1148 From CDavis at che-east.org Wed Oct 19 08:32:36 2016 From: CDavis at che-east.org (Cassie P. Davis) Date: Wed, 19 Oct 2016 13:32:36 +0000 Subject: [Histonet] clip-on magnifiers Message-ID: <5C815EADE724D14AA0CC8F037C4185F079B35981@SB01MSTMBX13.sb.trinity-health.org> RE: clip-on magnifiers Try Ted Pella Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org ________________________________________ From: histonet-request at lists.utsouthwestern.edu [histonet-request at lists.utsouthwestern.edu] Sent: Tuesday, October 18, 2016 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 155, Issue 15 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Clip-On Magnifiers for Embedding (Lisa Hamilton) 2. Beta-Catenin (Algeo, Lacie A) 3. Picrosirius Red (Bax, N.A.M.) ---------------------------------------------------------------------- Message: 1 Date: Mon, 17 Oct 2016 14:22:26 -0500 From: "Lisa Hamilton" To: Subject: [Histonet] Clip-On Magnifiers for Embedding Message-ID: <000901d228ab$cdacac90$690605b0$@com> Content-Type: text/plain; charset="us-ascii" In the past I have seen clip-on magnifiers that are used to embed tissue. Of course now that I need one I cannot remember what catalog I saw them in! I was hoping someone might know where I can find them. Thank You -Lisa ------------------------------ Message: 2 Date: Mon, 17 Oct 2016 19:53:28 +0000 From: "Algeo, Lacie A" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Beta-Catenin Message-ID: <24C4B3C167E5694887AB594C7602CE3A03CE1CCE at WN35104.or.providence.org> Content-Type: text/plain; charset="us-ascii" Hi All, What are people using for Beta-catenin control tissue? Is anyone using tissue that is known specifically to have a mutation for the beta-catenin gene? If so, is this something that is purchased? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo at providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. ------------------------------ Message: 3 Date: Tue, 18 Oct 2016 12:26:43 +0000 From: "Bax, N.A.M." To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Picrosirius Red Message-ID: <67502D640FCB0641AACF2BE17717060C325A9D1D at xserver30b.campus.tue.nl> Content-Type: text/plain; charset="us-ascii" Dear Histosearch, I just became a member and I hope you can provide me with advice. I'm really curious about the effect of not dissolving Direct Red 80 in 1.3% saturated Picric Acid but in 0.9-1.1% alkalimetric Picric acid solution. The reason why I'm curious is that I want to perform a staining on paraffin slides for Sirius Red and in my current lab they have Picrosirius red dissolved in 0.9-1.1% alkalimetric Picric acid, but the samples were I want to compare them to are stained in Picrosirius red made with the standard protocol with saturated Picric Acid. I measured the pH of the Picrosirius Red made with 0.9-1.1% alkalimetric Picric acid and this is ~pH 2.0 and all the Direct Red is dissolved, so can I assume that the staining will be comparable to Picrosirius Red dissolved in saturated Picric acid? Thank you very much in advance. Best Noortje Noortje AM Bax, PhD | Laboratory for Cell and Tissue Engineering | Biomedical Engineering | Eindhoven University of Technology, P.O. Box 513, Groene Loper, Gebouw 15, Gemini-Zuid 4.108, 5600 MB Eindhoven, The Netherlands | T: +31-40-2474830 | F: +31 40-2473744 |E-mail: n.a.m.bax at tue.nl| ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 155, Issue 15 ***************************************** Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From katarzyna.Winek at charite.de Wed Oct 19 09:25:23 2016 From: katarzyna.Winek at charite.de (Winek, Katarzyna) Date: Wed, 19 Oct 2016 14:25:23 +0000 Subject: [Histonet] IF Follicular Dendritic Cells Message-ID: Hello, I am trying to establish IF with cryosections (acetone fixed) from mouse spleen with FDC-M1 antibody from BD (551320). Can anyone recommend a good protocol? I have not been successful until now. Shall I switch to IHC? Best Kasia Katarzyna Winek * Doktorandin Klinik und Poliklinik f?r Neurologie Abteilung f?r Experimentelle Neurologie Charit? - Universit?tsmedizin Berlin Charit?platz 1 D-10117 Berlin Tel: 030 - 450 560 149 Fax: 030 - 450 560 915 katarzyna.winek at charite.de From Timothy.Morken at ucsf.edu Wed Oct 19 13:21:32 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 19 Oct 2016 18:21:32 +0000 Subject: [Histonet] JC requirement for stain QC question Message-ID: <761E2B5697F795489C8710BCC72141FF6FDA3B96@ex07.net.ucsf.edu> Hi all, for those under Joint Commission, can you give your interpretation of this standard having to do with QC of stains? _________________________ QSA 13.06.01 The equipment, methods and stains used in producing microscopic slides provides tissue sections that facilitate a diagnosis Elements of Performance A 1. A pathologist qualified in anatomic pathology assesses the staining quality (for example, equipment, methods, stains) of microscopic tissue sections to determine the stains ability to facilitate a diagnosis C 2. (D)The Laboratory performs quality controls on histologic stains for intended reactivity. The quality control results are documented. (m) Notes: for example, immunohistochemical (IHC) stains have positive and negative controls, and for periodic acid-Schiff 9PAS) stains, documentation C 3 (D) Each time of use for patient testing, the laboratory performs quality controls for each type of histologic stain used. The quality control results are documented. _______________________________ We always read this as the pathologist signs off on a validated stain procedure and the histotechs do the QC of stains (H&E and Special Stains) and document it. The pathologist does not necessarily review the daily QC slides. The inspector today says that the Pathologist has to review and sign off each daily QC slide. Does anyone have any experience other than what we normally do? Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken at ucsfmedctr.org From turkekul at gmail.com Wed Oct 19 16:45:20 2016 From: turkekul at gmail.com (Mesru T) Date: Wed, 19 Oct 2016 17:45:20 -0400 Subject: [Histonet] Histonet Digest, Vol 155, Issue 14 In-Reply-To: References: Message-ID: Hello Dear Friends, Our lab really likes the light sky blue color of Ventana Hematoxylin (as opposed to other more purple ones). I noticed the million types of Hemtatoxylins in the "Theory and Practice of Histotechnology" book. Does anybody knows a Hematoxylin that will give this blue color? Please help me, I would like to prepare it myself. Thanks, Mesruh Turekekul MSKCC.org Histology Lab From rsrichmond at gmail.com Wed Oct 19 18:42:47 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Wed, 19 Oct 2016 19:42:47 -0400 Subject: [Histonet] Magnifiers for Embedding Message-ID: >>Lisa Hamilton asks: In the past I have seen clip-on magnifiers that are used to embed tissue. Of course now that I need one I cannot remember what catalog I saw them in! I was hoping someone might.<< If you're looking for something that fits over your head and puts lenses in front of your eyes (including your glasses) then what you want is an OptiVISOR. I've used these for years for grossing, and at home for looking at small mineral specimens. About $30 from a number of vendors - I got my new one from Amazon not too long ago. For embedding you probably want 3 diopter lenses (lens number 3), which focus at about 14 inches. You can also get a light to mount on it, and a loupe to fit over one lens for additional magnification. OptiVISORs have optical glass lenses - some of the competitors have plastic lenses. (I have no commercial connection with anybody mentioned here.) Bob Richmond Samurai Pathologist Maryville TN From Fawn.Bomar at HalifaxRegional.com Thu Oct 20 10:22:41 2016 From: Fawn.Bomar at HalifaxRegional.com (Fawn Bomar) Date: Thu, 20 Oct 2016 15:22:41 +0000 Subject: [Histonet] Bachelor's degrees to sit for HTL Message-ID: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA20241C@EXCH-2K10.hrhs.com> Hi everyone, I was wondering if anyone would be able to help my co-worker and myself in trying to figure out exactly what we need to do to obtain a bachelor's degree that would qualify us to sit for the HTL. As of right now we both have associate's degrees and our HT certification. We both work full time and have children so we cannot attend college full time and would have to do most of it online if possible due to our location in South Boston, Virginia. We are trying to figure out how to blend online classes with the science classes that may have labs required that we would have to attend campus for. Another question we are having is what specific bachelor's degree do we have to obtain, does it have to be a biology degree or a science degree? Thanks for all your help, Fawn Bomar ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From JMacDonald at mtsac.edu Thu Oct 20 13:14:55 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Thu, 20 Oct 2016 11:14:55 -0700 Subject: [Histonet] Bachelor's degrees to sit for HTL In-Reply-To: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA20241C@EXCH-2K10.hrhs.com> References: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA20241C@EXCH-2K10.hrhs.com> Message-ID: Fawn, There is no specification as to what type of degree it must be. It can be any baccalaureate degree as long as you have at least 30 semester hours of biology and chemistry combined. I have had students with art and psychology degrees sit for the HTL exam. They came back to college to get the additional science classes to satisfy the HTL (ASCP) requirement. Jennifer From: Fawn Bomar via Histonet To: "histonet at lists.utsouthwestern.edu" Date: 10/20/2016 08:27 AM Subject: [Histonet] Bachelor's degrees to sit for HTL Hi everyone, I was wondering if anyone would be able to help my co-worker and myself in trying to figure out exactly what we need to do to obtain a bachelor's degree that would qualify us to sit for the HTL. As of right now we both have associate's degrees and our HT certification. We both work full time and have children so we cannot attend college full time and would have to do most of it online if possible due to our location in South Boston, Virginia. We are trying to figure out how to blend online classes with the science classes that may have labs required that we would have to attend campus for. Another question we are having is what specific bachelor's degree do we have to obtain, does it have to be a biology degree or a science degree? Thanks for all your help, Fawn Bomar ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly at merck.com Thu Oct 20 14:13:00 2016 From: brett_connolly at merck.com (Connolly, Brett M) Date: Thu, 20 Oct 2016 15:13:00 -0400 Subject: [Histonet] Books and Atlases- Part 2 (with pricing and more books added) Message-ID: Hi all, Thanks for all the responses to my previous post. I really want to pass these along rather than trash them. I updated the list and included my asking price. In addition I have a second list of free books - provided you purchase a book or books (one free book per purchase). I'll pay for shipping within the continental US. Couldn't be easier....please help me find a new home for these. I realize some are older, but histology and anatomy hasn't changed much. You can Google the ISBN# or copy and paste the ISBN# into Amazon Books ISBN locator for more info on these. I think they priced fairly, but I can negotiate. Here is the more recent list: Short Protocols in Molecular Biology 4thEd; ISBN 0-471-32938-X $25.00 Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology; ISBN 1-881299-43-0 SOLD Diagnostic Immunohistochemistry 2nd Ed; ISBN 0-443-06652-3 SOLD Atlas of Dermatopathology; ISBN 0-7216-4886-X $50.00 Atlas of UroSurgical Anatomy; ISBN 0-7216-3955-0 $30.00 Comparative Anatomy and Histology: A Mouse and Human atlas; ISBN 978-0-12-381361-9 $80.00 Human Histology 2nd Ed; ISBN 0-7234-2485-3 $40.00 A Color Atlas of Histology; ISBN 0-673-99190-3 $10.00 Color Atlas of Histology 3rd Ed; ISBN 0-683-30642-1 $10.00 Histology for Pathologists 2nd Ed; ISBN 0-397-51718-1 SOLD Immunochemistry (LabFax); ISBN1-872748-05-8 $12.00 Immunological Reagents & Solutions: A Laboratory Handbook ; ISBN 1-881299-29-5 $15.00 Atlas of Normal Histology 6th Ed; ISBN 0-8121-1126-5 $12.00 Histologic Preparations: Common Problems and Their Solutions; ISBN 978-0-930304-95-9 $50.00 Analytical Morphology: Theory, Applications & Protocols (soft cover); ISBN 1-881299-03-1 $15.00 A Color Atlas of Sectional Anatomy of the Mouse; ISBN 4- 900659-58-4 $50.00 Gray's Anatomy 13th Ed; ISBN 0-8121-0644-X $15.00 Primate Brain Maps: Structure of the Macaque Brain; ISBN 0-444-50415-X $50:00 At Atlas of Primate Gross Anatomy: Baboon, Chimpanzee, and Man ISBN 0-89874-321-4 $50.00 Color Atlas of Nonhuman Primate Histology; ISBN 3-8055-6879-7 $25.00 Structure of the Human Brain: A Photographic Atlas 3rd Ed: ISBN 0-19-504357-X $20.00 Atlas of the Rabbit Brain and Spinal Cord; ISBN 3-8055-3814-6 $40.00 Laboratory Methods in Histotechnology (AFIP- red cover); ISBN 1-881041-00-X $40.00 Here is the free book list: A Textbook of Histology 10th Ed; ISBN 0-7216-1757-3 Essential Immunology 6th Ed; ISBN 0-632-01994-8 Antibody Applications: Essential Techniques; ISBN 0-471-95698-8 Protein Localization by Fluorescence Microscopy: A Practical Approach; ISBN 0-19-963741-5 Techniques in Immunocytochemistry, Vol. 2; ISBN 0-12-140405-6 Tissue In Situ Hybridization: Methods in Animal Development; ISBN 0-471-16403-8 The Cell: Its Organelles and Inclusions - Atlas of Fine Structure; ISBN 0-7216-3585-7 Ham' s Histology 9th Ed; ISBN0-397-50681-3 Basic Histology 6th Ed: ISBN 0-8385-0575-9 The Human Brain: An introduction to Its Functional Anatomy 5th Ed; ISBN0-323-01320-1 Gross Pathology: A Color Atlas; ISBN 19-519797-6 Color Atlas of Histopathology; ISBN 0-19-519151-X Animal Processing Manual 1st Ed; Nat. Soc for Histotechnology Dictionary of Human Neuroanatomy ISBN 3-540-66523-4 Thanks, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From gmartin at marshallmedical.org Thu Oct 20 15:03:25 2016 From: gmartin at marshallmedical.org (Martin, Gary) Date: Thu, 20 Oct 2016 13:03:25 -0700 Subject: [Histonet] Position Message-ID: <6ED9D4252F278841A0593D3D788AF24C366E5E98@mailsvr.MARSHMED.local> We have a histo tech that is retiring in December, and we need to fill that position. We are a small lab that services a 100 bed hospital and the surrounding physicians. We are located in California at the base of the Sierra Foothills. If you are interested or know someone who may be interested, contact me. Gary Martin gmartin at marshallmedical.org. From aeck at dh.org Thu Oct 20 15:10:55 2016 From: aeck at dh.org (Eck, Allison) Date: Thu, 20 Oct 2016 20:10:55 +0000 Subject: [Histonet] open position Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A972FCA55@DH-MAIL01.dhorg.org> We currently have an open histology tech position. We are a busy lab in a community hospital in southeastern PA. If you are interested or know someone who may be interested, contact me or visit www.dh.org to apply Thanks Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT) Lead Tech Histology Doylestown Hospital 595 W State St Doylestown, PA 18901 215-345-2264 aeck at dh.org From Julia.Cates at AHSS.ORG Fri Oct 21 05:27:19 2016 From: Julia.Cates at AHSS.ORG (Cates, Julia) Date: Fri, 21 Oct 2016 10:27:19 +0000 Subject: [Histonet] JC requirement for stain QC question Message-ID: Hi Tim, We have a form here that was developed after a similar inspection. The form has quality control indicators for both the histologist and pathologist to review. At the end of the day, the pathologist will initial as reviewed for the entire form. He also documents QC in the patients report for Specials and IHC controls. Thanks, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Park Ridge Health (828)650-8243| Fax: (828)209-5315 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. From relia1 at earthlink.net Fri Oct 21 06:56:34 2016 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 21 Oct 2016 07:56:34 -0400 Subject: [Histonet] TGIF - Help me with my mental block! The "PA" dilemma... Message-ID: <041401d22b92$2bfb1320$83f13960$@earthlink.net> Hi Histonetters! This might seem like a dumb question but every time I get a position for a PA it seems that I can never remember if it should be pathologists' assistant or pathologist's assistant or pathologists assistant. Can somebody please clarify for me? And yes as you probably already guessed I am working on a PA position right now. It is in Tennessee. If anyone is interested please shoot me an email or give me a call for more details. I really need the answer to the question though. Thanks to all - have a great weekend!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From jaylundgren at gmail.com Fri Oct 21 11:09:33 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 21 Oct 2016 11:09:33 -0500 Subject: [Histonet] TGIF - Help me with my mental block! The "PA" dilemma... In-Reply-To: <041401d22b92$2bfb1320$83f13960$@earthlink.net> References: <041401d22b92$2bfb1320$83f13960$@earthlink.net> Message-ID: pathologists' On Fri, Oct 21, 2016 at 6:56 AM, Pam Barker via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Histonetters! > This might seem like a dumb question but every time I get a position for a > PA it seems that I can never remember if it should be pathologists' > assistant or pathologist's assistant or pathologists assistant. > Can somebody please clarify for me? > And yes as you probably already guessed I am working on a PA position right > now. It is in Tennessee. If anyone is interested please shoot me an email > or give me a call for more details. > > I really need the answer to the question though. > Thanks to all - have a great weekend!! > > > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > Pam M. Barker > > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1 at earthlink.net > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > www.facebook.com /PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From brett_connolly at merck.com Fri Oct 21 13:07:48 2016 From: brett_connolly at merck.com (Connolly, Brett M) Date: Fri, 21 Oct 2016 14:07:48 -0400 Subject: [Histonet] Part 3 - Book and Atlases they're going fast Message-ID: Hi all, Thanks for all of your responses! The list has shrunk considerably. Here is what left. I forgot to add the Sheehan & Hrapchak classic: Theory and Practice of Histotechnology (this one is a little worn---go figure). Atlas of UroSurgical Anatomy; ISBN 0-7216-3955-0 $30.00 Comparative Anatomy and Histology: A Mouse and Human atlas; ISBN 978-0-12-381361-9 $80.00 Human Histology 2nd Ed; ISBN 0-7234-2485-3 $40.00 Immunochemistry (LabFax); ISBN 1-872748-05-8 $12.00 Atlas of Normal Histology 6th Ed; ISBN 0-8121-1126-5 $12.00 Primate Brain Maps: Structure of the Macaque Brain; ISBN 0-444-50415-X $50:00 Structure of the Human Brain: A Photographic Atlas 3rd Ed: ISBN 0-19-504357-X $20.00 Theory and Practice of Histotechnology 2nd Ed; ISBN 0-8016-4573-5 $50.00 Free books A Textbook of Histology 10th Ed; ISBN 0-7216-1757-3 The Cell: Its Organelles and Inclusions - Atlas of Fine Structure; ISBN 0-7216-3585-7 Ham' s Histology 9th Ed; ISBN0-397-50681-3 Basic Histology 6th Ed: ISBN 0-8385-0575-9 The Human Brain: An introduction to Its Functional Anatomy 5th Ed; ISBN0-323-01320-1 Animal Processing Manual 1st Ed; Nat. Soc for Histotechnology Dictionary of Human Neuroanatomy ISBN 3-540-66523-4 Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From tbraud at holyredeemer.com Fri Oct 21 13:36:28 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 21 Oct 2016 18:36:28 +0000 Subject: [Histonet] Tissue Processor question Message-ID: <48E053DDF6CE074DB6A7414BA05403F8091E98@HRHEX02-HOS.holyredeemer.local> Hi fellow Histonetters- I'm looking for any feedback from working labs using Thermo's Excelsior Processor. If you can help, can you please also include the age and usage on your instrument? Thanks, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 From jshelley at sbpdiscovery.org Fri Oct 21 14:28:19 2016 From: jshelley at sbpdiscovery.org (John Shelley) Date: Fri, 21 Oct 2016 19:28:19 +0000 Subject: [Histonet] Plant tissue Message-ID: Happy Friday! I have given a request for service to me to do some plant tissue histology and I must admit this is a new area for me. I plan to do both paraffin and frozen sectioning and would like to ask if there may be anyone has a protocol on how to handle such tissue in regards to a tissue processing schedule and also steps to take for freezing. Any information would be greatly appreciated. Have a great weekend! Kind Regards! John J Shelley Histology Core Manager Sanford Burnham Prebys Medical Discovery Institute at Lake Nona From mills at 3scan.com Fri Oct 21 14:54:29 2016 From: mills at 3scan.com (Caroline Miller) Date: Fri, 21 Oct 2016 12:54:29 -0700 Subject: [Histonet] Plant tissue In-Reply-To: References: Message-ID: I am interested in this too! Please forward to all. What are the best resources for how to handle plant material for microscopic analysis? thanks! mills On Fri, Oct 21, 2016 at 12:28 PM, John Shelley via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Happy Friday! > > I have given a request for service to me to do some plant tissue histology > and I must admit this is a new area for me. I plan to do both paraffin and > frozen sectioning and would like to ask if there may be anyone has a > protocol on how to handle such tissue in regards to a tissue processing > schedule and also steps to take for freezing. Any information would be > greatly appreciated. > > Have a great weekend! > > Kind Regards! > > John J Shelley > Histology Core Manager > Sanford Burnham Prebys Medical Discovery Institute at Lake Nona > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From lldewe at gmail.com Fri Oct 21 14:59:07 2016 From: lldewe at gmail.com (Loralei Dewe) Date: Fri, 21 Oct 2016 12:59:07 -0700 Subject: [Histonet] Plant tissue In-Reply-To: References: Message-ID: You should contact David Carter at UC Riverside. It is his area of expertise! Loralei On Oct 21, 2016 12:55 PM, "Caroline Miller via Histonet" wrote: > I am interested in this too! Please forward to all. What are the best > resources for how to handle plant material for microscopic analysis? > > thanks! > mills > > On Fri, Oct 21, 2016 at 12:28 PM, John Shelley via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Happy Friday! > > > > I have given a request for service to me to do some plant tissue > histology > > and I must admit this is a new area for me. I plan to do both paraffin > and > > frozen sectioning and would like to ask if there may be anyone has a > > protocol on how to handle such tissue in regards to a tissue processing > > schedule and also steps to take for freezing. Any information would be > > greatly appreciated. > > > > Have a great weekend! > > > > Kind Regards! > > > > John J Shelley > > Histology Core Manager > > Sanford Burnham Prebys Medical Discovery Institute at Lake Nona > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Caroline Miller (mills) > Director of Histology > 3Scan.com > 415 2187297 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From susanbachus at verizon.net Fri Oct 21 17:06:23 2016 From: susanbachus at verizon.net (susanbachus at verizon.net) Date: Fri, 21 Oct 2016 18:06:23 -0400 Subject: [Histonet] aniline oil/Holzer stain In-Reply-To: References: <34C22BB94729434598D767D3F4EB95E0F48E109CFD@FWDCWPMSGCMS03.hca.corpad.net> Message-ID: <02B87EB1294B474BB00EEF3F6501CCDD@UserPC> I'm trying to locate aniline oil for the Holzer glial fiber stain. I purchased what was listed as aniline oil from ENG, but the first hint that something was wrong was that it was incompatible with the chloroform also included in the differentiating solution. Looking at the fine print on the bottle, I saw that it was actually "aniline oil, 2.5% aqueous", which I would have interpreted as only containing 2.5% water, except that it seemed to be completely aqueous (2.5% aniline?), inasmuch as it wouldn't mix with the chloroform. I can't find any other source online. I see that it is also called aminobenzine or phenylamine, but am also having trouble locating sources under those names. I realize aniline oil is relatively toxic, but am using it under a fume hood. I tried dipping slides separately in the (apparently aqueous) aniline and then chloroform, but still didn't get good differentiation. Can anyone suggest a source or a substitute? Many thanks for your help! Susan From paula at excaliburpathology.com Fri Oct 21 18:12:32 2016 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Fri, 21 Oct 2016 23:12:32 +0000 (UTC) Subject: [Histonet] aniline oil/Holzer stain In-Reply-To: <02B87EB1294B474BB00EEF3F6501CCDD@UserPC> References: <34C22BB94729434598D767D3F4EB95E0F48E109CFD@FWDCWPMSGCMS03.hca.corpad.net> <02B87EB1294B474BB00EEF3F6501CCDD@UserPC> Message-ID: <1873118519.725871.1477091552220@mail.yahoo.com> You could try adding aqueous NaOH slowly to what you have until you start getting tiny white droplets in the water. Then they will fall and form an oily layer below the water. Decant the water or use a separator funnel to drain the aniline oil out the bottom. ?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Susan Bachus via Histonet To: histonet at lists.utsouthwestern.edu Sent: Friday, October 21, 2016 5:28 PM Subject: [Histonet] aniline oil/Holzer stain I'm trying to locate aniline oil for the Holzer glial fiber stain.? I purchased what was listed as aniline oil from ENG, but the first hint that something was wrong was that it was incompatible with the chloroform also included in the differentiating solution.? Looking at the fine print on the bottle, I saw that it was actually "aniline oil, 2.5% aqueous", which I would have interpreted as only containing 2.5% water, except that it seemed to be completely aqueous (2.5% aniline?), inasmuch as it wouldn't mix with the chloroform.? I can't find any other source online.? I see that it is also called aminobenzine or phenylamine, but am also having trouble locating sources under those names.? I realize aniline oil is relatively toxic, but am using it under a fume hood.? I tried dipping slides separately in the (apparently aqueous) aniline and then chloroform, but still didn't get good differentiation.? Can anyone suggest a source or a substitute?? Many thanks for your help!? Susan _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew at shaw.ca Fri Oct 21 22:43:19 2016 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Fri, 21 Oct 2016 20:43:19 -0700 Subject: [Histonet] aniline oil/Holzer stain In-Reply-To: <02B87EB1294B474BB00EEF3F6501CCDD@UserPC> References: <34C22BB94729434598D767D3F4EB95E0F48E109CFD@FWDCWPMSGCMS03.hca.corpad.net> <02B87EB1294B474BB00EEF3F6501CCDD@UserPC> Message-ID: <580AE057.7060904@shaw.ca> Aniline oil is more commonly known just as aniline. Sigma list it for sale at: http://www.sigmaaldrich.com/catalog/search?term=aniline&interface=All&N=0&mode=match%20partialmax&lang=en®ion=CA&focus=product Bryan Llewellyn Susan Bachus via Histonet wrote: > I'm trying to locate aniline oil for the Holzer glial fiber stain. I > purchased what was listed as aniline oil from ENG, but the first hint > that something was wrong was that it was incompatible with the > chloroform also included in the differentiating solution. Looking at > the fine print on the bottle, I saw that it was actually "aniline oil, > 2.5% aqueous", which I would have interpreted as only containing 2.5% > water, except that it seemed to be completely aqueous (2.5% aniline?), > inasmuch as it wouldn't mix with the chloroform. I can't find any other > source online. I see that it is also called aminobenzine or > phenylamine, but am also having trouble locating sources under those > names. I realize aniline oil is relatively toxic, but am using it under > a fume hood. I tried dipping slides separately in the (apparently > aqueous) aniline and then chloroform, but still didn't get good > differentiation. Can anyone suggest a source or a substitute? Many > thanks for your help! Susan > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tabbott at pennstatehealth.psu.edu Mon Oct 24 09:36:15 2016 From: tabbott at pennstatehealth.psu.edu (Abbott, Tanya) Date: Mon, 24 Oct 2016 14:36:15 +0000 Subject: [Histonet] Cassette/slide printers Message-ID: Looking for suggestions on cassette and slide printers (either direct print or "paper" labels for slides) for a smaller, community hospital lab. Histo - 2 cutting stations, I Grossing area, average about 150 slides, 100 cassettes/day Cyto - average 40 ThinPreps, 5 FNAs/day Any input is appreciated, thanks in advance! Tanya Tanya G. Abbott HT(ASCP), RT(CSMLS) Pathology Manager Penn State Health St. Joseph Pathology Department From LRaff at uropartners.com Mon Oct 24 09:57:04 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 24 Oct 2016 14:57:04 +0000 Subject: [Histonet] Cassette/slide printers In-Reply-To: References: Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF110488F4@COLOEXCH01.uropartners.local> We are similar volume (slightly busier on some days.) WE use the Signature Model from Primera for both Cassettes and slides. I like the way our labels look. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: Abbott, Tanya via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, October 24, 2016 9:36 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cassette/slide printers Looking for suggestions on cassette and slide printers (either direct print or "paper" labels for slides) for a smaller, community hospital lab. Histo - 2 cutting stations, I Grossing area, average about 150 slides, 100 cassettes/day Cyto - average 40 ThinPreps, 5 FNAs/day Any input is appreciated, thanks in advance! Tanya Tanya G. Abbott HT(ASCP), RT(CSMLS) Pathology Manager Penn State Health St. Joseph Pathology Department _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Mon Oct 24 10:23:56 2016 From: patpxs at gmail.com (Patpxs) Date: Mon, 24 Oct 2016 08:23:56 -0700 Subject: [Histonet] Water for H&E Stainers? In-Reply-To: <14E2C6176416974295479C64A11CB9AE02BEEABA5B09@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE02BEEABA5B09@SBS2K8.premierlab.local> Message-ID: And the winner for the water of choice for H&E strainers is...... What ever you have available. Tap, tap with a filter, deionized. It all depends on the water quality in your area. Thanks to all who replied. Paula Sent from my iPhone > On Sep 27, 2016, at 9:54 AM, Elizabeth Chlipala wrote: > > Paula > > We have ours hooked up to tap water. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz at premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, September 27, 2016 10:28 AM > To: HistoNet > Subject: [Histonet] Water for H&E Stainers? > > Good Morning Everyone, > > > > What type of water do you use with your automated H&E stainers? House or deionized/distilled? > > > > We cannot buy one of the waterless, fancy schmancy H&E stainers at this time. L Thank you in advance. > > Sincerely, > > > > Paula > > > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly at merck.com Mon Oct 24 11:11:11 2016 From: brett_connolly at merck.com (Connolly, Brett M) Date: Mon, 24 Oct 2016 12:11:11 -0400 Subject: [Histonet] Part 4 - Book and Atlases - Final Message-ID: Hi all, Thanks for the tremendous response! I am delighted that 38 books and atlases have found new homes. I only have 2 left if there is still interest in these. Sorry - no more freebies available. Primate Brain Maps: Structure of the Macaque Brain; ISBN 0-444-50415-X $50:00 Atlas of UroSurgical Anatomy; ISBN 0-7216-3955-0 $30.00 Best regards, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From abadesuyi at nrh-ok.com Mon Oct 24 12:12:25 2016 From: abadesuyi at nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Mon, 24 Oct 2016 12:12:25 -0500 Subject: [Histonet] Histology lab assistant Message-ID: <04EE4F75BB5FB246ADB68D69B7460443B641372AD7@MAIL.nrhnt.nrh-ok.com> Hi, I am in the process of hiring a new histology lab assistant and will really appreciate it, if you can give me the idea of what their job descriptions should be. Best regards, Adesupo Adesuyi ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From james.fortune at unitypoint.org Tue Oct 25 10:26:33 2016 From: james.fortune at unitypoint.org (Fortune, James A.) Date: Tue, 25 Oct 2016 15:26:33 +0000 Subject: [Histonet] CBG 9L Freshtake Solvent recycler Message-ID: Hey Histonetters, A couple of weeks ago our CBG solvent recycler died and it is a costly repair. So instead of making costly repairs that may or may not last long, we are opting to purchase a new recycler. We are looking at CBG's benchtop model, our old unit was large but was very reliable for over 10 years. Has anyone out there used a benchtop model before. Are there any pro's or con's we should be looking at? The model number of the one we are looking at is MSLV-14-U-9-A 9L freshtake. Does anyone have this specific model that can share any likes or dislikes? Thanks for your help! Andy Fortune Tech specialist Unitypoint Health, Meriter Laboratories This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. SSSS 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Jaime.Reuss at cchmc.org Tue Oct 25 13:21:26 2016 From: Jaime.Reuss at cchmc.org (Reuss, Jaime) Date: Tue, 25 Oct 2016 18:21:26 +0000 Subject: [Histonet] Core transplants in TMA's Message-ID: I am currently working on creating TMA's using a semi-automated machine. I punch all recipient and donor cores and transplant as well. When transplanting, even though I've set the distance from the recipient block to the puncher accurately, it still seems the core is being smashed when I transplant it. Any ideas or suggestions? Should I re-embed the donor blocks before or is the problem in the initial coring? Thanks! email: Jaime.reuss at cchmc.org From sprice2003 at gmail.com Tue Oct 25 18:51:07 2016 From: sprice2003 at gmail.com (Sally Price) Date: Tue, 25 Oct 2016 19:51:07 -0400 Subject: [Histonet] South Carolina Society for Histotechnology Fall Expo Message-ID: Hey folks ? I'm definitely not as good at promoting a meeting as Billie Zimmerman is but I wouldn't want anyone to overlook their opportunity to participate in the SCSH's Expo, which will be held the weekend of November 4 - 6 in beautiful Pawleys Island, just a short drive north of Charleston and south of Myrtle Beach. This may be your last chance to obtain much-needed CEU's before the end of the year, unless you enjoy those boring on-line programs. For more information, please contact SCSH's President, Chad McMahan, who can be reached at < clmcmah at clemson.edu >. Hope to see y'all there! -- Sally Price From relia1 at earthlink.net Wed Oct 26 09:28:40 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 26 Oct 2016 10:28:40 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin 10-26-2016 Trick or Treat!! Happy Halloween from Pam Barker at RELIA Solutions Message-ID: <00b301d22f95$3f7328f0$be597ad0$@earthlink.net> Hi Histonetters, Halloween is just around the corner. TRICK or TREAT!!!!! The TRICK is finding the right job opportunity for you. The TREAT is with my help it can be relatively painless. I will help you with your resume, Coach you through the interview and offer process And refer you to positions based on the criteria you give me. All of the positions I work with are fulltime permanent positions with some of the best facilities Nationwide. My clients offer excellent compensation including competitive salaries, great benefits, relocation assistance/sign on bonuses. I have exciting opportunities in these areas! Roanoke, VA Flagstaff, AZ Austin, TX Modesto, CA Manitowoc, WI Kingsport, TN San Diego, CA Chattanooga, TN (Pathologists' Assistant) If you or any of your friends would like more information on any of these positions or if you would like to discuss opportunities in other areas or future job searches please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or on my cell/text at 407-353-5070 or at relia1 at earthlink.net Remember I offer over 20 years of recruiting and employment counseling experience, knowledgeable, confidential and responsive service to you and your friends and a permanent placement practice dedicated to the histology profession. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember. It never hurts to keep an eye open, even if you are happy in your present job. p.s. How are you celebrating Halloween? Happy Halloween!!!!!!!!!!!! Pam - 866-607-3542 (866-60RELIA) Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From aonomic at auburn.edu Wed Oct 26 09:56:04 2016 From: aonomic at auburn.edu (Michelle Aono) Date: Wed, 26 Oct 2016 14:56:04 +0000 Subject: [Histonet] Microtome Cleaning Company and What to do with Old Metal Blades Message-ID: <3fb2b935b20548eb8ee482a785fa48b8@ex7.auburn.edu> Can anyone suggest a good company that works on old microtomes? Ours is not broken, it's just in need of some TLC. Also, what is a reasonable price for such work (we have a RJ 2040)? Also, we have a lot of the old metal blades that you have to sharpen. Any suggestions on how to sell, donate, recycle such items? Do people even use these anymore? I would hate to have them thrown out. Thanks! Michelle (Shelly) Aono ~~~~~~~~~~~~~~~~~~~ Research Associate II 107B/124 Greene Hall Auburn University, Dept of APP Auburn, AL 36849 (334) 844-5594 From rjbuesa at yahoo.com Wed Oct 26 10:15:45 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 26 Oct 2016 15:15:45 +0000 (UTC) Subject: [Histonet] Microtome Cleaning Company and What to do with Old Metal Blades In-Reply-To: <3fb2b935b20548eb8ee482a785fa48b8@ex7.auburn.edu> References: <3fb2b935b20548eb8ee482a785fa48b8@ex7.auburn.edu> Message-ID: <1912680441.1459904.1477494945268@mail.yahoo.com> I sold all my old steel blades on eBay.Ren? On Wednesday, October 26, 2016 11:13 AM, Michelle Aono via Histonet wrote: Can anyone suggest a good company that works on old microtomes?? Ours is not broken, it's just in need of some TLC.? Also, what is a reasonable price for such work (we have a RJ 2040)? Also, we have a lot of the old metal blades that you have to sharpen.? Any suggestions on how to sell, donate, recycle such items?? Do people even use these anymore?? I would hate to have them thrown out. Thanks! Michelle (Shelly) Aono ~~~~~~~~~~~~~~~~~~~ Research Associate II 107B/124 Greene Hall Auburn University, Dept of APP Auburn, AL 36849 (334) 844-5594 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From 41dmb41 at gmail.com Wed Oct 26 11:59:09 2016 From: 41dmb41 at gmail.com (Drew Meyer) Date: Wed, 26 Oct 2016 12:59:09 -0400 Subject: [Histonet] Microtome Cleaning Company and What to do with Old Metal Blades In-Reply-To: <3fb2b935b20548eb8ee482a785fa48b8@ex7.auburn.edu> References: <3fb2b935b20548eb8ee482a785fa48b8@ex7.auburn.edu> Message-ID: Michael Dietrich and his team at Southeast Pathology Instrument Service is outstanding! www.southeastpathology.com Thanks, Drew Meyer, HT Anatomic Pathology Independent Contractor On Wed, Oct 26, 2016 at 10:56 AM, Michelle Aono via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Can anyone suggest a good company that works on old microtomes? Ours is > not broken, it's just in need of some TLC. Also, what is a reasonable > price for such work (we have a RJ 2040)? > > Also, we have a lot of the old metal blades that you have to sharpen. Any > suggestions on how to sell, donate, recycle such items? Do people even use > these anymore? I would hate to have them thrown out. > > Thanks! > Michelle (Shelly) Aono > ~~~~~~~~~~~~~~~~~~~ > Research Associate II > 107B/124 Greene Hall > Auburn University, Dept of APP > Auburn, AL 36849 > (334) 844-5594 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cfields at mlkch.org Wed Oct 26 12:25:11 2016 From: cfields at mlkch.org (Carol G Fields) Date: Wed, 26 Oct 2016 17:25:11 +0000 Subject: [Histonet] Microtome Cleaning Company and What to do with Old Metal Blades In-Reply-To: References: <3fb2b935b20548eb8ee482a785fa48b8@ex7.auburn.edu> Message-ID: <438ef0ce625240fc8b301dca32e891e8@mlk01exc02.mlkch.org> Hi, I have used Michael Dietrich and his team at Southeast Pathology Instrument Servicefor years. They are the best and the nicest! Carole Carole Fields, HT (ASCP) Lead Histotechnologist, Pathology Laboratory Martin Luther King Jr. Community Hospital 1680 E. 120th Street Los Angeles, CA 90059 O:424-338-8000 x 8341 cfields at mlkch.org -----Original Message----- From: Drew Meyer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, October 26, 2016 9:59 AM To: Michelle Aono Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome Cleaning Company and What to do with Old Metal Blades Michael Dietrich and his team at Southeast Pathology Instrument Service is outstanding! www.southeastpathology.com Thanks, Drew Meyer, HT Anatomic Pathology Independent Contractor On Wed, Oct 26, 2016 at 10:56 AM, Michelle Aono via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Can anyone suggest a good company that works on old microtomes? Ours > is not broken, it's just in need of some TLC. Also, what is a > reasonable price for such work (we have a RJ 2040)? > > Also, we have a lot of the old metal blades that you have to sharpen. > Any suggestions on how to sell, donate, recycle such items? Do people > even use these anymore? I would hate to have them thrown out. > > Thanks! > Michelle (Shelly) Aono > ~~~~~~~~~~~~~~~~~~~ > Research Associate II > 107B/124 Greene Hall > Auburn University, Dept of APP > Auburn, AL 36849 > (334) 844-5594 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mburns at atlanticurologyclinics.com Wed Oct 26 12:42:37 2016 From: mburns at atlanticurologyclinics.com (Melissa Burns) Date: Wed, 26 Oct 2016 17:42:37 +0000 Subject: [Histonet] IHC Instrument Message-ID: <8C537C608EA0194B87F6DE7BC6E1BF88423EEDE5@AUC-Exchange2.gsuro.com> We are looking to get rid of a Ventana Benchmark XT. Anyone have suggestions? I've tried a few places I know buy used equipment. We need the room ASAP for other things. Feel free to e-mail with suggestions. Thank you so much, Melissa Burns From jriggleman at globusmedical.com Wed Oct 26 15:25:01 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Wed, 26 Oct 2016 20:25:01 +0000 Subject: [Histonet] Microtome Cleaning Company and What to do with Old Metal Blades In-Reply-To: <3fb2b935b20548eb8ee482a785fa48b8@ex7.auburn.edu> References: <3fb2b935b20548eb8ee482a785fa48b8@ex7.auburn.edu> Message-ID: Hello, I send mine out to IMEB in California. You may be able to sell the blades on Ebay. Thank you, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. -----Original Message----- From: Michelle Aono [mailto:aonomic at auburn.edu] Sent: Wednesday, October 26, 2016 10:56 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Microtome Cleaning Company and What to do with Old Metal Blades Can anyone suggest a good company that works on old microtomes? Ours is not broken, it's just in need of some TLC. Also, what is a reasonable price for such work (we have a RJ 2040)? Also, we have a lot of the old metal blades that you have to sharpen. Any suggestions on how to sell, donate, recycle such items? Do people even use these anymore? I would hate to have them thrown out. Thanks! Michelle (Shelly) Aono ~~~~~~~~~~~~~~~~~~~ Research Associate II 107B/124 Greene Hall Auburn University, Dept of APP Auburn, AL 36849 (334) 844-5594 From PAMarcum at uams.edu Thu Oct 27 11:09:11 2016 From: PAMarcum at uams.edu (Marcum, Pamela A) Date: Thu, 27 Oct 2016 16:09:11 +0000 Subject: [Histonet] Small Slide Dryer or Oven Message-ID: <5a9115e8ee664fb087dd7e87b3f449d1@MAIL13M2N1.ad.uams.edu> We are looking for a small slide dryer (hold up to three 30 position short racks) that will reach 65C and when opened return to temperature within 1 or 2 minutes. It does not have to fancy and is only used for special stains so it is not a constant use item. Pam ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Forest.Blankenship at dchstx.org Thu Oct 27 11:39:27 2016 From: Forest.Blankenship at dchstx.org (Forest Blankenship) Date: Thu, 27 Oct 2016 16:39:27 +0000 Subject: [Histonet] Cassette and slide labelers Message-ID: <8D5A5A0BF8A80F43A6E52872E56F523C013A5AD7@dchmxdb02.driscoll.dch> Hi there Histotechs, I am the manager of a low volume laboratory that is looking into cassette and slide labelers. Many of the units are too large for our space and it looks like Thermo and General Data are the best fits for our lab moving into a Beaker LIS future. I would appreciate any feedback on the General Data system (they took over Shurmark) especially in a Beaker environment. We are in build mode for Beaker and leaving CoPath because the rest of the hospital is using Epic. Thank you, Forest "Kevin" Blankenship, HTL(ASCP) Manager Anatomic Pathology Driscoll Children's Hospital Disclaimer: This email and its content are confidential and intended solely for the use of the addressee. Please notify the sender if you have received this email in error or simply delete it. From lmarie08 at uga.edu Thu Oct 27 11:55:06 2016 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Thu, 27 Oct 2016 16:55:06 +0000 Subject: [Histonet] bone saw Message-ID: Hello histoworld, Does anyone out there use a bone saw in their lab? We routinely have research cases with hundreds of femur head submissions from avian species. We currently use a bone saw made by Buehler from the 70's or 80's and it's a work horse, but the blade keeps cracking in the diamond tip from overuse during these surveys of hundreds of bones. I was wondering what kind of saws are out there that could be used for this purpose and if anyone has any experience with this? I am looking for something a little more durable, or if not, at least a little cheaper. Each blade costs about $350. Thanks! From tbraud at holyredeemer.com Thu Oct 27 12:33:46 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 27 Oct 2016 17:33:46 +0000 Subject: [Histonet] Bone saw Message-ID: <48E053DDF6CE074DB6A7414BA05403F8092E11@HRHEX02-HOS.holyredeemer.local> We use an awesome little band saw made by IMEB, Inc. It has a small foot print, 4 blade types and added accessories for a super lab bone cutting station, and best of all, very inexpensive. It can zip through the densest of bone, or the most delicate. It can be set up as a water cooled station to reduce dust particulate, but we just have ours under a hood (It's that tiny!) and we use a standard blade. Our pathologists and PA LOVE it, and so do the techs, because we get such fabulously decaled thin, consistent sections. Hope this helps, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 -----Original Message----- Sent: Thursday, October 27, 2016 1:00 PM To: histonet at lists.utsouthwestern.edu Today's Topics: 6. bone saw (Lauren Sweeney) ---------------------------------------------------------------------- Message: 6 Date: Thu, 27 Oct 2016 16:55:06 +0000 From: Lauren Sweeney Subject: [Histonet] bone saw Hello histoworld, Does anyone out there use a bone saw in their lab? We routinely have research cases with hundreds of femur head submissions from avian species. We currently use a bone saw made by Buehler from the 70's or 80's and it's a work horse, but the blade keeps cracking in the diamond tip from overuse during these surveys of hundreds of bones. I was wondering what kind of saws are out there that could be used for this purpose and if anyone has any experience with this? I am looking for something a little more durable, or if not, at least a little cheaper. Each blade costs about $350. Thanks! From Timothy.Morken at ucsf.edu Thu Oct 27 12:37:52 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 27 Oct 2016 17:37:52 +0000 Subject: [Histonet] Bone saw In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8092E11@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8092E11@HRHEX02-HOS.holyredeemer.local> Message-ID: <761E2B5697F795489C8710BCC72141FF6FDA5871@ex07.net.ucsf.edu> I agree, we use the IMEB bone saw as well. All human bones though... -----Original Message----- From: Terri Braud via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, October 27, 2016 10:34 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Bone saw We use an awesome little band saw made by IMEB, Inc. It has a small foot print, 4 blade types and added accessories for a super lab bone cutting station, and best of all, very inexpensive. It can zip through the densest of bone, or the most delicate. It can be set up as a water cooled station to reduce dust particulate, but we just have ours under a hood (It's that tiny!) and we use a standard blade. Our pathologists and PA LOVE it, and so do the techs, because we get such fabulously decaled thin, consistent sections. Hope this helps, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 -----Original Message----- Sent: Thursday, October 27, 2016 1:00 PM To: histonet at lists.utsouthwestern.edu Today's Topics: 6. bone saw (Lauren Sweeney) ---------------------------------------------------------------------- Message: 6 Date: Thu, 27 Oct 2016 16:55:06 +0000 From: Lauren Sweeney Subject: [Histonet] bone saw Hello histoworld, Does anyone out there use a bone saw in their lab? We routinely have research cases with hundreds of femur head submissions from avian species. We currently use a bone saw made by Buehler from the 70's or 80's and it's a work horse, but the blade keeps cracking in the diamond tip from overuse during these surveys of hundreds of bones. I was wondering what kind of saws are out there that could be used for this purpose and if anyone has any experience with this? I am looking for something a little more durable, or if not, at least a little cheaper. Each blade costs about $350. Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Forest.Blankenship at dchstx.org Thu Oct 27 13:21:35 2016 From: Forest.Blankenship at dchstx.org (Forest Blankenship) Date: Thu, 27 Oct 2016 18:21:35 +0000 Subject: [Histonet] Thank you Message-ID: <8D5A5A0BF8A80F43A6E52872E56F523C013A5B3A@dchmxdb02.driscoll.dch> Thank you all for the input on printers for cassettes and labelers. Kevin Disclaimer: This email and its content are confidential and intended solely for the use of the addressee. Please notify the sender if you have received this email in error or simply delete it. From rcharles at pa.gov Fri Oct 28 09:35:17 2016 From: rcharles at pa.gov (Charles, Roger) Date: Fri, 28 Oct 2016 14:35:17 +0000 Subject: [Histonet] eye lens Message-ID: <58184e186cc54084bb41fb3570ccf9b2@ENCTCEXCH024.PA.LCL> Hello, I have a block of dog eye lens in which I can get very nice sections. When I lay it out on my waterbath it wrinkles back up. Is there something I can do to keep this from happening or is there another method of mounting eye lens to a slide that someone can share. Thanks Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us From rsrichmond at gmail.com Fri Oct 28 23:34:20 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 29 Oct 2016 00:34:20 -0400 Subject: [Histonet] Bone saw Message-ID: > > Terri L. Braud, HT(ASCP). Anatomic Pathology Supervisor, Holy Redeemer > Hospital, Meadowbrook PA describes: > > >>We use an awesome little band saw made by IMEB, Inc. It has a small > foot print, 4 blade types and added accessories for a super lab bone > cutting station, and best of all, very inexpensive. > It can zip through the densest of bone, or the most delicate. It can be > set up as a water cooled station to reduce dust particulate, but we just > have ours under a hood (It's that tiny!) and we use a standard blade. > Our pathologists and PA LOVE it, and so do the techs, because we get such > fabulously decaled thin, consistent sections.<< > Is this the item you're describing? http://www.imebinc.com/necropsy-morgue/imeb-bone-band-saw.html $1,600 each? No way is a pathologist going to be allowed one of these! With a new gig, I usually go to a hardware store and buy a hacksaw, and leave it behind at the job when I'm done. Bob Richmond Samurai Pathologist Maryville TN From raestask at grics.net Sat Oct 29 07:22:54 2016 From: raestask at grics.net (raestask at grics.net) Date: Sat, 29 Oct 2016 08:22:54 -0400 (EDT) Subject: [Histonet] Control block for asbestos In-Reply-To: <43655927.83678040.1477743660664.JavaMail.root@grics.net> Message-ID: <639545796.83679400.1477743774418.JavaMail.root@grics.net> Does anyone have or know where to obtain control slides for Perl's Iron stain for asbestos? Thanks in advance, Rae A Staskiewicz HT (ASCP) UnityPoint Health| Methodist|Proctor From JRobinson at pathology-associates.com Mon Oct 31 11:50:12 2016 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Mon, 31 Oct 2016 16:50:12 +0000 Subject: [Histonet] link was lost Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C98E79494@PAEXCH1.PathologyAssociates.local> Subscribe, please. Jeff Robinson, Sierra Pathology Lab, Clovis, CA. This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you.