From LRaff at uropartners.com Tue Nov 1 07:37:06 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 1 Nov 2016 12:37:06 +0000 Subject: [Histonet] Happy November---Lab related blog post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF1106EBE0@COLOEXCH01.uropartners.local> Hope everyone had a good Halloween and all are looking forward to the holiday season. Today's blog takes a look at lab orientation for non-labratorians. http://www.chicagonow.com/downsize-maybe/2016/11/labra-cadabra-getting-the-right-laboratory-result-to-the-right-patient-at-the-right-time/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From cls71877 at gmail.com Tue Nov 1 07:47:43 2016 From: cls71877 at gmail.com (Cristi Rigazio) Date: Tue, 1 Nov 2016 08:47:43 -0400 Subject: [Histonet] Cold Ischemic time Message-ID: Hi histo land! Referencing CAP requirement ANP.22983, how do you all document cold ischemic time? Do you have a "macro" that the pathologists input or a disclaimer? We were recently inspected and we include a "disclaimer" in the reports that the time is less than one hour, but the inspector said that it was not "Accurately documented in the report"...I want to fix this this, of course but wondered if you all had any guidance such that I don't try and recreate the wheel. Thank you for your time! Cristi From Julia.Cates at AHSS.ORG Tue Nov 1 12:27:16 2016 From: Julia.Cates at AHSS.ORG (Cates, Julia) Date: Tue, 1 Nov 2016 17:27:16 +0000 Subject: [Histonet] Cold Ischemic time Message-ID: Hi Cristi, We have the PA/Pathologist dictate the Ischemic time and total fixation time at the end of the gross. It reads something like, "The specimen has an ischemic time of 1 hour and is allowed to fix for 48 hours". As long as it documents both times you should be fine. Thanks, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Park Ridge Health (828)650-8243| Fax: (828)209-5315 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. This message (including any attachments) is intended only for the use of the individual or entity to which it is addressed and may contain information that is non-public, proprietary, privileged, confidential, and exempt from disclosure under applicable law or may constitute as attorney work product. If you are not the intended recipient, you are hereby notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, notify us immediately by telephone and (i) destroy this message if a facsimile or (ii) delete this message immediately if this is an electronic communication. Thank you. From tbraud at holyredeemer.com Tue Nov 1 12:30:07 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 1 Nov 2016 17:30:07 +0000 Subject: [Histonet] cold ischemic time Message-ID: <48E053DDF6CE074DB6A7414BA05403F809488D@HRHEX02-HOS.holyredeemer.local> Hi Cristi - We have a large breast practice and we worked with the surgeons and surgical staff to include on the requisition: "Collect time" "Time in formalin" Then at gross, the PA or pathologist grossing calculates "Cold Ischemic Time" and "Duration of Fixation" All breast specimens are handled as STAT so that larger specimens can be dissected for a block of any residual tumor to be placed in Fixative. If this is done, we calculate "Cold Ischemic Time" and "Time in Formalin" from that moment. This is all reported through a Macro. If there is any deviation from the fixation guidelines (which are also printed on the report through the same macro) then we record the deviation and disclaimer through the "Notes" section on the report. This is all laid out in our policy/procedure for breast reporting. I hope this helps, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Today's Topics: 2. Cold Ischemic time (Cristi Rigazio) Message: 2 Date: Tue, 1 Nov 2016 08:47:43 -0400 From: Cristi Rigazio Subject: [Histonet] Cold Ischemic time Hi histo land! Referencing CAP requirement ANP.22983, how do you all document cold ischemic time? Do you have a "macro" that the pathologists input or a disclaimer? We were recently inspected and we include a "disclaimer" in the reports that the time is less than one hour, but the inspector said that it was not "Accurately documented in the report"...I want to fix this this, of course but wondered if you all had any guidance such that I don't try and recreate the wheel. Thank you for your time! Cristi From gmartin at marshallmedical.org Tue Nov 1 12:45:02 2016 From: gmartin at marshallmedical.org (Martin, Gary) Date: Tue, 1 Nov 2016 10:45:02 -0700 Subject: [Histonet] Position open Message-ID: <6ED9D4252F278841A0593D3D788AF24C366E5EBC@mailsvr.MARSHMED.local> Haven't had a response to this open position ... so I'll try one more time; We have a histo tech that is retiring in December, and we need to fill that position. We are a small lab that services a 100 bed hospital and the surrounding physicians. We are located in California at the base of the Sierra Foothills. If you are interested or know someone who may be interested, contact me. Thanks, Gary Martin gmartin at marshallmedical.org. From owensk2 at ccf.org Tue Nov 1 13:06:55 2016 From: owensk2 at ccf.org (Owens, Katrina) Date: Tue, 1 Nov 2016 18:06:55 +0000 Subject: [Histonet] CryoJane sectioning of Bone Message-ID: <51E813F67A862847A3DEA0781684F8919CCF57@CC-CLEXMB58.cc.ad.cchs.net> Hey Histonet people! I was wondering if anyone had done any CryoJane sectioning of undecalified bone and if you had, do you have any tips or tricks you'd be willing to share? Thanks so much! Katrina Owens owensk2 at ccf.org =================================== Please consider the environment before printing this e-mail Cleveland Clinic is ranked as one of the top hospitals in America by U.S.News & World Report (2015). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. From Miller.Wendy at mhsil.com Tue Nov 1 13:21:22 2016 From: Miller.Wendy at mhsil.com (Miller, Wendy) Date: Tue, 1 Nov 2016 18:21:22 +0000 Subject: [Histonet] H&E 600 Message-ID: <1478024482534.27517@mhsil.com> Good afternoon! I was wondering if anyone out there is using the new Roche stainer/coverslipper, the H&E 600? We are possibly considering purchasing one and wanted to ask if anyone out there is currently using this model, and if so, what are your thoughts? Thank you, Wendy Miller, HTL Memorial Medical Center Springfield, IL 62781 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Blanca.Lopez at UTSouthwestern.edu Tue Nov 1 13:26:02 2016 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Tue, 1 Nov 2016 18:26:02 +0000 Subject: [Histonet] Mouse to mouse kit Message-ID: <7dabbb0bce6d427fa7e8154761654171@SWMS13MAIL12.swmed.org> Hello!!! Histonets, I was wondering if somebody can help me in how to use mouse to mouse kit to perform IHC. I have a Dako autostainer only. thanks Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From Lacie.Algeo at providence.org Tue Nov 1 14:10:16 2016 From: Lacie.Algeo at providence.org (Algeo, Lacie A) Date: Tue, 1 Nov 2016 19:10:16 +0000 Subject: [Histonet] processing cell blocks in formalin Message-ID: <24C4B3C167E5694887AB594C7602CE3A03CE673F@WN35104.or.providence.org> Hi All, Does anyone have a procedure for processing cellblocks in formalin that works well? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo at providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From Richard.Cartun at hhchealth.org Tue Nov 1 15:25:20 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 1 Nov 2016 20:25:20 +0000 Subject: [Histonet] processing cell blocks in formalin In-Reply-To: <24C4B3C167E5694887AB594C7602CE3A03CE673F@WN35104.or.providence.org> References: <24C4B3C167E5694887AB594C7602CE3A03CE673F@WN35104.or.providence.org> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E953A89E0@HHCEXCHMB03.hhcsystem.org> Yes, I will send you the procedure that we use. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Algeo, Lacie A via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 01, 2016 3:10 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] processing cell blocks in formalin Hi All, Does anyone have a procedure for processing cellblocks in formalin that works well? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo at providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From craigak12 at gmail.com Tue Nov 1 16:16:50 2016 From: craigak12 at gmail.com (J B) Date: Tue, 01 Nov 2016 21:16:50 +0000 Subject: [Histonet] Specimen Return Policy: Message-ID: Can someone please share their Specimen Return Policy? Our lab is trying to put something together for patients requesting tissue, hardware, etc. Do most people return tissue, bone, and hardware to patients? Please advise. Sincerely, JB From lmarie08 at uga.edu Wed Nov 2 08:45:44 2016 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Wed, 2 Nov 2016 13:45:44 +0000 Subject: [Histonet] ASCP subtest exam scores Message-ID: Does anyone out there who has taken the HT or HTL know what the subtest scores mean, how many points they are out of? It says what your total score is out of 400 but then the subtest scores are all high numbers that do not add up to 400, they add up to more so how do you interpret them? From Valerie.Hannen at parrishmed.com Wed Nov 2 09:58:51 2016 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Wed, 2 Nov 2016 10:58:51 -0400 Subject: [Histonet] Cold Ischemic time In-Reply-To: References: Message-ID: <450B7A81EDA0C54E97C53D60F00776C323E455E663@isexstore03> We had a rubber stamp made that we use on the heading of the breast when we get the specimen. It looks like this : Time Tissue Obtained:_________ Time Tissue Placed in Formalin________ Total Time Tissue in Formalin__________ Cold Ischemia: Met/ NOT met (we circle the appropriate one) All of this information is included on the bottom of the Pathology report. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Cristi Rigazio via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 01, 2016 8:48 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cold Ischemic time Hi histo land! Referencing CAP requirement ANP.22983, how do you all document cold ischemic time? Do you have a "macro" that the pathologists input or a disclaimer? We were recently inspected and we include a "disclaimer" in the reports that the time is less than one hour, but the inspector said that it was not "Accurately documented in the report"...I want to fix this this, of course but wondered if you all had any guidance such that I don't try and recreate the wheel. Thank you for your time! Cristi _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From johnronan at custompathlabsolutions.com Wed Nov 2 13:47:14 2016 From: johnronan at custompathlabsolutions.com (John Ronan) Date: Wed, 2 Nov 2016 14:47:14 -0400 Subject: [Histonet] Used Equipment In-Reply-To: References: Message-ID: Hi Histonetters, I worked at an in-office histology lab at a urology practice and it closed last year. All equipment was sold except an oven and a sakura stainer. Both are in excellent condition and the practice is looking to sell them quickly to repurpose the space. Anyone interested in pictures or further info please email me at johnronan at custompathlabsolutions.com From gagnone at KGH.KARI.NET Wed Nov 2 14:21:17 2016 From: gagnone at KGH.KARI.NET (Gagnon, Eric) Date: Wed, 2 Nov 2016 19:21:17 +0000 Subject: [Histonet] White Benchkote product Message-ID: <5F06C3AD0B27264CA20CFA986C87882E01BB66DDD0@EXCHANGEPV1.KGH.ON.CA> Is anyone in Histoland using white bench-covering products, either in roll form or in sheets? (These are white, shiny-coated on one side, and have the consistency of filter paper on the other.) In the past, we have taped them to benchtops to provide a clean look. Are these still in widespread use, and if so, have you encountered any infection control issues arising from their use i.e. during inspections or with your IC folks? Thanks in advance, Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From mverdu at histopat.es Thu Nov 3 07:53:26 2016 From: mverdu at histopat.es (=?UTF-8?Q?Montse_Verd=C3=BA?=) Date: Thu, 3 Nov 2016 13:53:26 +0100 Subject: [Histonet] coverslipper Message-ID: <75a8b2aeecce3406575da034b35c1ff6@mail.gmail.com> Ai everybody, We are looking at options for automated glass coverslipper workstations. Actually we worked with CV5030 Leyca, but the service is not very efficient. We must renew coverslipper and would like to know your experience. Any suggestion??? Thanks!!! Montse Verd? Histopat laboratoris Tel +34932033000 Biopat. Biopatologia Molecular Tel+34932030837 mverdu at histopat.es This message and its attachments are addressed exclusively to the person or entity indicated as its named addressee, and may contain data and/or confidential information, subject to professional secret or whose disclosure is prohibited by virtue of current legislation in force. Any distribution, disclosure or reproduction of this type by persons or entities other than the addressee is strictly prohibited. If you have received this message by error, please contact the person indicated as its sender and delete it. From lmarie08 at uga.edu Thu Nov 3 09:06:15 2016 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Thu, 3 Nov 2016 14:06:15 +0000 Subject: [Histonet] product review Message-ID: Hello histoland, Has anyone out there used Midsci products before? We are considering getting slides from them.... Thanks! From CBird at amli-denton.com Thu Nov 3 09:23:47 2016 From: CBird at amli-denton.com (Cindy Bird) Date: Thu, 3 Nov 2016 09:23:47 -0500 Subject: [Histonet] (no subject) Message-ID: Anyone know where I can find the "old" special stain description sheets? The ones we used back in the day when studying to take exam. Cindy Bird From relia1 at earthlink.net Thu Nov 3 09:27:56 2016 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 3 Nov 2016 10:27:56 -0400 Subject: [Histonet] How to Handle Political Conversations in the Workplace - a great read for current times. Message-ID: <00eb01d235de$78abe9f0$6a03bdd0$@earthlink.net> Hi Histopeeps, I hope everybody is having a great day. I came across this article on Glassdoor.com and had to share it since it is so timely!! https://www.glassdoor.com/blog/politics-at-work/?utm_source=newsletter&utm_m edium=email&utm_content=how_to_politics_US16&utm_campaign=nov16_US Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Lacie.Algeo at providence.org Thu Nov 3 10:57:18 2016 From: Lacie.Algeo at providence.org (Algeo, Lacie A) Date: Thu, 3 Nov 2016 15:57:18 +0000 Subject: [Histonet] block retention Message-ID: <24C4B3C167E5694887AB594C7602CE3A03CE6F4B@WN35104.or.providence.org> Hi All, Outside of the current guidelines, how long is everyone saving their blocks/slides. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo at providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From Lacie.Algeo at providence.org Thu Nov 3 11:12:20 2016 From: Lacie.Algeo at providence.org (Algeo, Lacie A) Date: Thu, 3 Nov 2016 16:12:20 +0000 Subject: [Histonet] Her2 IHC Message-ID: <24C4B3C167E5694887AB594C7602CE3A03CE6F84@WN35104.or.providence.org> Are any labs reflexing 1+ for FISH? Thanks :) Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo at providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From srishan at mail.holyname.org Thu Nov 3 11:15:28 2016 From: srishan at mail.holyname.org (srishan at mail.holyname.org) Date: Thu, 3 Nov 2016 12:15:28 -0400 Subject: [Histonet] OVER DECALCYFIED TISSUE Message-ID: Does anyone has any procedure for restoring good nuclear staining for an over decalcified tissue? If anyone has any solutions please share, it will be greatly appreciated. Mala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From marktarango at gmail.com Thu Nov 3 13:56:30 2016 From: marktarango at gmail.com (Mark Tarango) Date: Thu, 3 Nov 2016 11:56:30 -0700 Subject: [Histonet] Her2 IHC In-Reply-To: <24C4B3C167E5694887AB594C7602CE3A03CE6F84@WN35104.or.providence.org> References: <24C4B3C167E5694887AB594C7602CE3A03CE6F84@WN35104.or.providence.org> Message-ID: My lab does not have a written policy on this yet but are discussing it. If the patient has a high grade tumor and HER2 IHC is 1+ (or even 0+), some of our pathologists will reflex to FISH. The oncologists will request it sometimes too, more often when ER and PR are both negative. We have found several cases to be positive by FISH that were 0+ or 1+ by IHC. I can't say anything about response to HER2 therapy in this group of patients just that they met the criteria for positive by FISH. Mark T. Cellnetix Seattle, Wa On Thu, Nov 3, 2016 at 9:12 AM, Algeo, Lacie A via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Are any labs reflexing 1+ for FISH? > Thanks :) > Lacie > > Lacie Algeo, HTL (ASCP) MBCM > Histology Supervisor > Providence Sacred Heart Medical Center Laboratory > 101 W 8th Avenue > L-2 > Spokane, WA 99204 > 509-474-4418 > FAX 509-474-2052 > lacie.algeo at providence.org > > > This message is intended for the sole use of the addressee, and may > contain information that is priviledged, confidential and exempt from > disclosure under applicable law. If you are not the addressee, you are > hereby notified that you may not use, copy, disclose or distribute to > anyone the message or any information contained in the message. If you > have received this message in error, please immediately advise the sender > by reply e-mail and delete this message. > > > ________________________________ > > This message is intended for the sole use of the addressee, and may > contain information that is privileged, confidential and exempt from > disclosure under applicable law. If you are not the addressee you are > hereby notified that you may not use, copy, disclose, or distribute to > anyone the message or any information contained in the message. If you have > received this message in error, please immediately advise the sender by > reply email and delete this message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shinwari_1 at yahoo.com Fri Nov 4 10:36:54 2016 From: shinwari_1 at yahoo.com (Nasir Abbas) Date: Fri, 4 Nov 2016 15:36:54 +0000 (UTC) Subject: [Histonet] Suggestion for Dual staining Kit/reagent References: <735822620.281524.1478273814874.ref@mail.yahoo.com> Message-ID: <735822620.281524.1478273814874@mail.yahoo.com> Hi All Can some one suggest dual staining reagents for IHC-P (mouse liver tissue) for my primary antibodies: ab183685, GTX53126 and ab25377.I was suggested vectors labs products (following table) but I am not clear about Vector Blue reagent SK-5300 that whether it can be used in IHC-P, because it features shows it is fluorescent. Moreover it is sensitive to xylene and Histoclear may be used as a clearing reagent in this case, which makes me even more worried. Any suggestion and tips for my dual staining?Table: | Pri-Ab | Vector Lab Products- Suggested | | ab183685 | MP-7401-15 | | GTX53126 | SK-4105 | | ab25377 | MP-5404-15 | | ? | SK-5300 | ? Sincerely?Nasir Abbas ---PhD FellowGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan Avenue?Science Park?Guangzhou, 510530,?Guangdong?P.R.ChinaCell: +86-1312 8296 614 From Helena.Howe at ProMedica.org Fri Nov 4 12:16:19 2016 From: Helena.Howe at ProMedica.org (Howe, Helena) Date: Fri, 4 Nov 2016 17:16:19 +0000 Subject: [Histonet] Transporting wet tissues Message-ID: Does anyone have a system for transporting large specimens to Your lab. We are building an offsite lab for Anatomic Pathology and are looking for a safe way to transport the large specimens that are in buckets of formalin. _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ EMAIL CONFIDENTIALITY NOTICE This Email message, and any attachments, may contain confidential patient health information that is legally protected. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this message is strictly prohibited. If you have received this information in error, please notify the sender immediately by replying to this message and delete the message from your system. From Timothy.Morken at ucsf.edu Fri Nov 4 12:25:59 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 4 Nov 2016 17:25:59 +0000 Subject: [Histonet] Transporting wet tissues In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF6FDA77A4@ex07.net.ucsf.edu> Helen, we use Coleman 50-qt wheeled coolers (with wheels and handles). The containers and buckets are parafilmed and put in plastic bags. The coolers have formalin absorbing pads at the bottom for any leaks. The coolers are liquid proof, easy to roll around. Occasionally a handle will break on one, but we keep spares at each site. We use these for couriering all our paperwork and specimens between 3 sites. We don't courier too many large specimens, but we do courier a lot of cassettes in baskets for processing from two sites to a central histo lab for processing. Same issue - containing the formalin in a container. https://www.amazon.com/Coleman-50-Quart-Wheeled-Cooler-Blue/dp/B000G33YT0/ref=sr_1_2?ie=UTF8&qid=1478280100&sr=8-2&keywords=50+qt+wheeled+cooler Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Howe, Helena via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, November 04, 2016 10:16 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Transporting wet tissues Does anyone have a system for transporting large specimens to Your lab. We are building an offsite lab for Anatomic Pathology and are looking for a safe way to transport the large specimens that are in buckets of formalin. _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ EMAIL CONFIDENTIALITY NOTICE This Email message, and any attachments, may contain confidential patient health information that is legally protected. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this message is strictly prohibited. If you have received this information in error, please notify the sender immediately by replying to this message and delete the message from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Fri Nov 4 12:43:32 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 4 Nov 2016 17:43:32 +0000 Subject: [Histonet] Her2 Reflex Message-ID: <48E053DDF6CE074DB6A7414BA05403F810E7FA@HRHEX03-HOS.holyredeemer.local> Re: Her2 by IHC We reflex 1+ to FISH, too Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 From patpxs at gmail.com Fri Nov 4 14:27:48 2016 From: patpxs at gmail.com (P Sicurello) Date: Fri, 4 Nov 2016 12:27:48 -0700 Subject: [Histonet] In need of a temporary histotech in San Diego Message-ID: Good Afternoon, We are looking for an experienced histotech (HT/HTL certified) who can work as a temp full-time. We need this person to start the week of November 14, 2016. If this is you, please contact me. My work email is psicurello at ucsd.edu, my phone # is 619-543-2872. Sorry for the short notice, but stuff happens, you know what I mean? Happy Friday. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist/Interim Supervisor Histology, EM, IHC UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From kmilne at bccrc.ca Fri Nov 4 14:35:12 2016 From: kmilne at bccrc.ca (Katy Milne) Date: Fri, 4 Nov 2016 19:35:12 +0000 Subject: [Histonet] Suggestion for Dual staining Kit/ In-Reply-To: References: Message-ID: Hi Nasir, I use Biocare reagents (DAB, Warp Red and Ferengi Blue) for my multicolour stuff. Even though the Vector blue fluoresces you should still see what you need to see in brightfield. If you're worried about losing your signal through solvents you could probably just airdry before coverslipping, that's what I do (using Ecomount). The xyelene is only a problem after you put the chromogen on, not before. Looks like you have 2 rabbit and 1 rat Ab for your Abs, it may be possible to do a triple stain with that with a rat and rabbit in the first round and the other rabbit in the 2nd or you should be able to combine at least a rat and rabbit in a double simultaneous or 2 rabbits in a double sequential stain depending on your Abs and how robust they are. If you don't have access to spectral imaging though it may be safer going with doubles so you can pull everything apart by eye. Is that CD8 Ab behaving ok for you? Looks like it has a ton of background in the images. If you're having issues with it you may be able to combine your CD4 Ab with the CD3 SP7 clone to show the presumptive CD8s. I haven't worked with any of those Abs but I do do multicolour IHC in both human and mouse tissues so if you need help sorting out protocols let me know, of course I can only speak to Biocare reagents for this as that's what I usually use. Katy Milne Deeley Research Centre Message: 2 Date: Fri, 4 Nov 2016 15:36:54 +0000 (UTC) From: Nasir Abbas To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Suggestion for Dual staining Kit/reagent Message-ID: <735822620.281524.1478273814874 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Hi All Can some one suggest dual staining reagents for IHC-P (mouse liver tissue) for my primary antibodies: ab183685, GTX53126 and ab25377.I was suggested vectors labs products (following table) but I am not clear about Vector Blue reagent SK-5300 that whether it can be used in IHC-P, because it features shows it is fluorescent. Moreover it is sensitive to xylene and Histoclear may be used as a clearing reagent in this case, which makes me even more worried. Any suggestion and tips for my dual staining?Table: | Pri-Ab | Vector Lab Products- Suggested | | ab183685 | MP-7401-15 | | GTX53126 | SK-4105 | | ab25377 | MP-5404-15 | | ? | SK-5300 | ? Sincerely?Nasir Abbas From blayjorge at gmail.com Sun Nov 6 09:28:18 2016 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Sun, 6 Nov 2016 10:28:18 -0500 Subject: [Histonet] Use VHS to record scientific information Message-ID: Dear Colleagues: In this age of digital media, how common is to use VHS (= video home system, analog medium) tapes to capture scientific information (e.g. histological or of any other kind) that will then be analyzed? How common do you think was the use of VHS tapes in science some 20 years ago? If you could quantify your answers, great. What type of information was (and/or is) being captured with those methods (e.g. anatomical, physiological, behavioral, etc.)? If you have a constructive reply, please do so off the listserver to my email: blayjorge at gmail.com Apologies for potential duplicate emails. Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From criley at dpspa.com Mon Nov 7 06:56:19 2016 From: criley at dpspa.com (Charles Riley) Date: Mon, 7 Nov 2016 07:56:19 -0500 Subject: [Histonet] Automated Stainers Message-ID: Does anyone have any automated stainers with programmable stain options for sale? We used to have the Stainmate stainers but they are all breaking down and looking to replace them with something similar without having to spend too much money as I am on a strict budget at the moment. If anyone has one or two and or any suggestions for stainers please let me know as soon as you can. Thank you all in advance for any responses sent my way -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From criley at dpspa.com Mon Nov 7 07:35:29 2016 From: criley at dpspa.com (Charles Riley) Date: Mon, 7 Nov 2016 08:35:29 -0500 Subject: [Histonet] Automated stainer Update Message-ID: Sorry to flood your e-mails but I wanted to send an update to my last e-mail. I am looking for something small that can be placed in a mobile van laboratory. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From Blanca.Lopez at UTSouthwestern.edu Mon Nov 7 09:27:53 2016 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Mon, 7 Nov 2016 15:27:53 +0000 Subject: [Histonet] Microtome needed Message-ID: <53a985d39e21481981a38c23e1e89881@SWMS13MAIL12.swmed.org> Histoneths!!! I have a doctor looking to buy a Histology instruments mainly microtome used or new. Does somebody knows any company or vendor that I can contact? thanks Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From PREISZNE at mail.etsu.edu Mon Nov 7 09:28:58 2016 From: PREISZNE at mail.etsu.edu (Preiszner, Johanna) Date: Mon, 7 Nov 2016 15:28:58 +0000 Subject: [Histonet] anybody familiar with molecular sieves? Message-ID: Hi, we recycle our ethanol and get back a lot of 92-93% ethanol. I looked up molecular sieves/ desiccants and what I see these can be used to selectively adsorb water from water/ethanol mixtures and the sieve/desiccant can be regenerated and used multiple times. If it worked we could save a lot of money by buying less 100% ethanol. Could someone let me know why it would or would not work? Thanks, Hann Preiszner ETSU/QCOM From POWELL_SA at mercer.edu Mon Nov 7 09:59:25 2016 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Mon, 7 Nov 2016 15:59:25 +0000 Subject: [Histonet] Microtome needed In-Reply-To: <53a985d39e21481981a38c23e1e89881@SWMS13MAIL12.swmed.org> References: <53a985d39e21481981a38c23e1e89881@SWMS13MAIL12.swmed.org> Message-ID: Try Pathology Services, Inc, or PSI, in Thomaston GA. These guys are great. They have a few for sale at good prices. I have copied this to their service manager, Bonney Lane, or you may contact them at 1-866 -398-9478, or www.pathologyserv.com. Shirley Powell -----Original Message----- From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, November 07, 2016 10:28 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Microtome needed Histoneths!!! I have a doctor looking to buy a Histology instruments mainly microtome used or new. Does somebody knows any company or vendor that I can contact? thanks Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bbarnard at personifysearch.com Mon Nov 7 10:34:55 2016 From: bbarnard at personifysearch.com (Blake Barnard) Date: Mon, 7 Nov 2016 11:34:55 -0500 Subject: [Histonet] Career Opportunity - Advanced Field Support Specialist Message-ID: Hello Histonet, Our exclusively retained client has opened a new Advanced Field Applications/Support Specialist opportunity and I wanted to reach out regarding a few specifics and see if anyone would be interested in learning more. Our client is a global leader in cancer diagnostics and they are looking for an advanced field based applications/support role. The ideal candidate will have a strong background and understanding of Immunohistochemistry (IHC) and histology. This individual will provide technical training on use of systems and must be comfortable presenting to customers at all levels. The company is part of a $9 billion organization and is growing. This position is a full-time, direct-hire role with competitive salary and full benefits. If you or anyone you know may be interested in learning more about this position, please contact me directly at bbarnard at personifysearch.com. Thank you! *Blake Barnard* Talent Management Executive [image: https://docs.google.com/a/personifysearch.com/uc?id=0ByHopg_78n39ZklCMWpubm40VDQ&export=download] *Personify * 416 S. Dawson St. Raleigh, NC 27601 (Direct) 252-299-5876 www.personifysearch.com [image: https://docs.google.com/a/personifysearch.com/uc?id=0ByHopg_78n39QWJEcGwtanJSM0E&export=download] [image: https://docs.google.com/a/personifysearch.com/uc?id=0ByHopg_78n39YThBR2pfd1czVEk&export=download] [image: https://docs.google.com/a/personifysearch.com/uc?id=0ByHopg_78n39WFhrYjIyak84ZE0&export=download] [image: https://docs.google.com/a/personifysearch.com/uc?id=0ByHopg_78n39RmQ4NndwWFNteGs&export=download] From carl.hobbs at kcl.ac.uk Tue Nov 8 05:44:47 2016 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Tue, 8 Nov 2016 11:44:47 +0000 Subject: [Histonet] PGP 9.5 Ab x-reactivity Message-ID: Hi I'd be grateful if anyone can confirm whether or not Dako's Z511601-2 or Leica's ( Novacastra's) PGP9.5-L-CE anti PGP 9.5 antibodies work on mouse/rat Formalin-fixed Paraffin wax sections. Thank you Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From TGainey at bmhsc.org Tue Nov 8 10:12:56 2016 From: TGainey at bmhsc.org (Teresa W. Gainey) Date: Tue, 8 Nov 2016 16:12:56 +0000 Subject: [Histonet] block retention In-Reply-To: <24C4B3C167E5694887AB594C7602CE3A03CE6F4B@WN35104.or.providence.org> References: <24C4B3C167E5694887AB594C7602CE3A03CE6F4B@WN35104.or.providence.org> Message-ID: We keep our blocks for 15 years. Slides forever....per Pathologist request. Teresa W.Gainey Beaufort Memorial Hospital Pathology Team Coordinator 843-522-5159 ________________________________ From: Algeo, Lacie A Sent: Thursday, November 3, 2016 11:57 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] block retention Hi All, Outside of the current guidelines, how long is everyone saving their blocks/slides. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo at providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From Shannon.Logan at bellin.org Tue Nov 8 10:25:14 2016 From: Shannon.Logan at bellin.org (Logan, Shannon) Date: Tue, 8 Nov 2016 16:25:14 +0000 Subject: [Histonet] block retention In-Reply-To: References: <24C4B3C167E5694887AB594C7602CE3A03CE6F4B@WN35104.or.providence.org> Message-ID: <86b28afca3dc426497a9818cff6c0473@BAPWEXCH001a.bellin.com> We keep our blocks and slides for 10 years. Shannon H. Logan HTL (ASCP) Pathology Dept. Bellin Health 744 S Webster Ave Green Bay, WI 54305 920-433-3653 -----Original Message----- From: Teresa W. Gainey via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 08, 2016 10:13 AM To: Algeo, Lacie A; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] block retention We keep our blocks for 15 years. Slides forever....per Pathologist request. Teresa W.Gainey Beaufort Memorial Hospital Pathology Team Coordinator 843-522-5159 ________________________________ From: Algeo, Lacie A Sent: Thursday, November 3, 2016 11:57 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] block retention Hi All, Outside of the current guidelines, how long is everyone saving their blocks/slides. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo at providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jclark at pcnm.com Tue Nov 8 11:35:04 2016 From: jclark at pcnm.com (Joanne Clark) Date: Tue, 8 Nov 2016 17:35:04 +0000 Subject: [Histonet] Competency for Supervisors Message-ID: Good Morning Histonetters, how do all you histology supervisors out in Histoland do competency on yourselves? Do any of you have a good form to evaluate your performance both on the bench and as an administrator? Anything will be of assistance. Thank you! Joanne Clark, HT(ASCP) Director of Histology Pathology Consultants of New Mexico 575-622-5600 Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From foreightl at gmail.com Tue Nov 8 15:10:37 2016 From: foreightl at gmail.com (Patrick Laurie) Date: Tue, 8 Nov 2016 16:10:37 -0500 Subject: [Histonet] A suitable negative control for BAP1 Message-ID: Hello everyone, We recently brought BAP1 into our IHC menu, and we were eventually able to get a good stain that our pathologist was happy with. We are, however, looking for a good suitable negative control. Our pathologist is not certain and wanted me to ask the local community. Thanks, Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 From mills at 3scan.com Tue Nov 8 16:02:40 2016 From: mills at 3scan.com (Caroline Miller) Date: Tue, 8 Nov 2016 14:02:40 -0800 Subject: [Histonet] PGP 9.5 Ab x-reactivity In-Reply-To: References: Message-ID: I am interested in this too please! However I will be performing the stain whole mount on skin and gut Thanks in advance! yours, mills On Tue, Nov 8, 2016 at 3:44 AM, Hobbs, Carl via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > Hi > I'd be grateful if anyone can confirm whether or not Dako's Z511601-2 or > Leica's ( Novacastra's) PGP9.5-L-CE anti PGP 9.5 antibodies work on > mouse/rat Formalin-fixed Paraffin wax sections. > > Thank you > > > Carl > > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > 020 7848 6813 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From akemiat3377 at gmail.com Tue Nov 8 18:07:20 2016 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Tue, 8 Nov 2016 16:07:20 -0800 Subject: [Histonet] block retention In-Reply-To: References: <24C4B3C167E5694887AB594C7602CE3A03CE6F4B@WN35104.or.providence.org> Message-ID: 10 years for all slides and blocks with the exception of pediatric cases. Those cases we keep forever. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com Tele: (831) 375-3577 X117 Cell: (408) 335-9994 > On Nov 8, 2016, at 8:12 AM, Teresa W. Gainey via Histonet wrote: > > We keep our blocks for 15 years. Slides forever....per Pathologist request. > > > Teresa W.Gainey > > Beaufort Memorial Hospital > > Pathology Team Coordinator > > 843-522-5159 > > > ________________________________ > From: Algeo, Lacie A > Sent: Thursday, November 3, 2016 11:57 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] block retention > > Hi All, > Outside of the current guidelines, how long is everyone saving their blocks/slides. > Thanks, > Lacie > > Lacie Algeo, HTL (ASCP) MBCM > Histology Supervisor > Providence Sacred Heart Medical Center Laboratory > 101 W 8th Avenue > L-2 > Spokane, WA 99204 > 509-474-4418 > FAX 509-474-2052 > lacie.algeo at providence.org > > > This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. > > > ________________________________ > > This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl at gmail.com Wed Nov 9 08:07:40 2016 From: foreightl at gmail.com (Patrick Laurie) Date: Wed, 9 Nov 2016 09:07:40 -0500 Subject: [Histonet] Fwd: A suitable negative control for BAP1 In-Reply-To: References: Message-ID: Hello everyone, We recently brought BAP1 into our IHC menu, and we were eventually able to get a good stain that our pathologist was happy with. We are, however, looking for a good suitable negative control. Our pathologist is not certain and wanted me to ask the local community. Thanks, Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 From jriggleman at globusmedical.com Wed Nov 9 14:28:11 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Wed, 9 Nov 2016 20:28:11 +0000 Subject: [Histonet] Paraffin Removal Message-ID: Hi Everyone, Please tell me your tricks of removing paraffin from containers, lab benchtops, etc?.. I would really appreciate it! Thank you, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From mwerdler at gmail.com Wed Nov 9 15:00:05 2016 From: mwerdler at gmail.com (Mca Werdler) Date: Wed, 9 Nov 2016 15:00:05 -0600 Subject: [Histonet] Chicken samples Message-ID: Hello Everyone, I have a co worker who is wondering if it is possible to use mouse or rat antibodies on chicken tissue? Could someone help him with this, i have no idea how to help him Thank you From relia1 at earthlink.net Thu Nov 10 09:59:42 2016 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 10 Nov 2016 10:59:42 -0500 Subject: [Histonet] RELIA Hot Histology Job Alert! Dayshift Histotech position in FL!!! Message-ID: <0b0401d23b6b$73de65a0$5b9b30e0$@earthlink.net> Hi Histonetters! I hope you are having a great day. I just wanted to send a quick post to let you know about an exciting opportunity I am currently working on. I have a client, a growing derm lab, located in SW Florida that has a newly created position for a grossing histotech. This is a full time permanent dayshift position. They are looking for someone with a Florida license who is CLIA qualified to gross. If you or someone you know is interested in hearing more about this opportunity I can be reached toll free at 866-607-3542 or cell/text 407-353-5070 or via email at relia1 at earthlink.net. Remember if I place someone that you refer you will earn a referral fee. Thanks for taking the time to read my post. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From TGainey at bmhsc.org Thu Nov 10 11:12:50 2016 From: TGainey at bmhsc.org (Teresa W. Gainey) Date: Thu, 10 Nov 2016 17:12:50 +0000 Subject: [Histonet] Histology Team Leader position available in Beaufort, South Carolina Message-ID: <581ab56aded94d34907f6ee2e6d6765c@bmhsc.org> Full time Histology Team Leader Position is available in the beautiful low country at Beaufort Memorial Hospital in Beaufort, South Carolina. This is a full time day position with no nights or weekends. If interested please contact Sharon Anderson, Lab Director. Phone 843-522-5129. Job application is available on the BMHSC.org website. From michang2014 at gmail.com Thu Nov 10 14:12:14 2016 From: michang2014 at gmail.com (Michelle Chang) Date: Thu, 10 Nov 2016 12:12:14 -0800 Subject: [Histonet] Chicken samples Message-ID: Hi, The reactivity of the antibodies depend on the epitope/immunogen of the antibodies. I would advice to check % homology of the eptiope/immunogen to that of chicken to know if the antibodies work for on chicken. High homology means it is very much likely that it will work. Thank you Michelle From Hlukey at msn.com Thu Nov 10 16:00:37 2016 From: Hlukey at msn.com (Hugh Luk) Date: Thu, 10 Nov 2016 22:00:37 +0000 Subject: [Histonet] A suitable negative control for BAP1 In-Reply-To: References: Message-ID: Patrick, We've been studying BAP-1 too and do not have a good answer for a truly negative tissue, except for skeletal muscle, but decided against it. Our current multi control block contains: 1) lung ca, 2) tonsil, 3) colon ca, 4) LN, 5) ovary ca, 6) lung. Our clone specifically stains each tissue, either nuclear or cytoplasmic. My pathologists agonized over this for awhile, before they were happy with this mix. Hugh-Hawaii ________________________________ Date: Tue, 8 Nov 2016 16:10:37 -0500 From: Patrick Laurie Subject: [Histonet] A suitable negative control for BAP1 Hello everyone, We recently brought BAP1 into our IHC menu, and we were eventually able to get a good stain that our pathologist was happy with. We are, however, looking for a good suitable negative control. Our pathologist is not certain and wanted me to ask the local community. Thanks, Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 ------------------------------ From LRaff at uropartners.com Fri Nov 11 09:52:33 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 11 Nov 2016 15:52:33 +0000 Subject: [Histonet] Lab Related Blog Post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF1109622A@COLOEXCH01.uropartners.local> Hope everyone has a good weekend! http://www.chicagonow.com/downsize-maybe/2016/11/you-can-be-a-whistle-blower-or-you-can-look-for-a-different-way/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From blayjorge at gmail.com Sun Nov 13 10:45:11 2016 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Sun, 13 Nov 2016 11:45:11 -0500 Subject: [Histonet] Brief (2-3 minutes) survey on respiration in terrestrial insects Message-ID: Dear Colleagues: Would you mind taking a brief survey on respiration in terrestrial insects? https://www.surveymonkey.com/r/KDZL8BT Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From smclaughlin7 at cooleydickinson.org Mon Nov 14 10:05:41 2016 From: smclaughlin7 at cooleydickinson.org (McLaughlin, Stacy L.) Date: Mon, 14 Nov 2016 16:05:41 +0000 Subject: [Histonet] Fw: Cellmarque H.Pylori In-Reply-To: References: Message-ID: From: McLaughlin, Stacy L. Sent: Thursday, November 10, 2016 3:44 PM To: histonet at lists.utsouthwestern.edu Subject: Cellmarque H.Pylori Hello Histoland! I was just informed that Cellmarque's polyclonal H.Pylori is on backorder with no ETA for release. Has anyone else experienced this problem and are there other vendors you would recommend? Thank you. Stacy McLaughlin, HT (ASCP) Histology Supervisor Cooley Dickinson Healthcare 30 Locust Street Northampton, MA 01060 (413-582-2019 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From sandra.cheasty at wisc.edu Mon Nov 14 14:08:59 2016 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Mon, 14 Nov 2016 20:08:59 +0000 Subject: [Histonet] Thyroglobulin Ab-6 (Clone TGB04+TGB05) Message-ID: Hello all! Does anyone have a source for Thyroglobulin Ab-6 (Clone TGB04+TGB05), 200ug/ml? We use it primarily on canine and feline, (It also works on guinea pigs,). Thanks much! Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From IMYLES at PARTNERS.ORG Mon Nov 14 14:25:31 2016 From: IMYLES at PARTNERS.ORG (Myles, Irene) Date: Mon, 14 Nov 2016 20:25:31 +0000 Subject: [Histonet] Congo Red Controls Message-ID: <32DFC0C34EFB774B822FDBB5434AAFBC0119971627@PHSX10MB17.partners.org> Good Afternoon Histonet, How does everyone handle their Congo Red Controls; thickness, room temp or refridgerator, how long do they last once cut, keep them baked or unbaked and anything else I did not list. Thank you very much. Irene Irene Myles, HT (ASCP) Senior Histology Technician Brigham and Women's Hospital Histology; Dept. of Pathology Amory Bldg; 3rd flr; RM 368J 75 Francis Street Boston, MA 02215 617-732-5454 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From patpxs at gmail.com Mon Nov 14 15:56:48 2016 From: patpxs at gmail.com (Paula Sicurello) Date: Mon, 14 Nov 2016 21:56:48 +0000 (UTC) Subject: [Histonet] Part Time Histologist Needed In San Diego, California References: <792733265.3534652.1479160608507.ref@mail.yahoo.com> Message-ID: <792733265.3534652.1479160608507@mail.yahoo.com> Hello Listers, UC San Diego Health has an opening for a part-time Histologist.? HT/HTL required. Experience in a high volume academic setting preferred.? We need someone with strong sectioning and trouble shooting skills.? Familiarity with IHC or Electron Microscopy a plus. Go to jobs.ucsd.edu and look for job #84828 Sincerely, ?Paula ?PaulaSicurello, HTL (ASCP)CM InterimSupervisor: EM, Histology, IHC UCSan Diego Health 200Arbor Drive SanDiego, CA 92103 (P):619-543-2872 psicurello at ucsd.edu ? Confidentiality Notice: The information transmitted in this e-mailis intended only for the person or entity to which it is addressed and maycontain confidential and/or privileged material.? Any review,retransmission, dissemination or other use of or taking of any action inreliance upon this information by persons or entities other than the intendedrecipient is prohibited.? If you received this e-mail in error, pleasecontact the sender and delete the material from any computer. From JMacDonald at mtsac.edu Mon Nov 14 17:13:04 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Mon, 14 Nov 2016 15:13:04 -0800 Subject: [Histonet] Congo Red Controls In-Reply-To: <32DFC0C34EFB774B822FDBB5434AAFBC0119971627@PHSX10MB17.partners.org> References: <32DFC0C34EFB774B822FDBB5434AAFBC0119971627@PHSX10MB17.partners.org> Message-ID: We cut ours at 8 um within a couple of days of when we are going to use them. We bake them as usual. From: "Myles, Irene via Histonet" To: "'histonet at lists.utsouthwestern.edu'" Date: 11/14/2016 12:26 PM Subject: [Histonet] Congo Red Controls Good Afternoon Histonet, How does everyone handle their Congo Red Controls; thickness, room temp or refridgerator, how long do they last once cut, keep them baked or unbaked and anything else I did not list. Thank you very much. Irene Irene Myles, HT (ASCP) Senior Histology Technician Brigham and Women's Hospital Histology; Dept. of Pathology Amory Bldg; 3rd flr; RM 368J 75 Francis Street Boston, MA 02215 617-732-5454 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aaditza1 at gmail.com Wed Nov 16 09:23:46 2016 From: aaditza1 at gmail.com (Aditza A) Date: Wed, 16 Nov 2016 09:23:46 -0600 Subject: [Histonet] Tissue Proccessor Thermo Scientific STP 120-3 feedback Message-ID: Dear all, Can you give me some feedback about Tissue Proccessor Thermo Scientific STP 120-3? We are a small research lab and wounder if anyone uses this one and how good is .... https://www.thermofisher.com/order/catalog/product/813150 Sincerely, Adriana Rosca HTL(ASCP),MS Health Communication "...what we say and how we say it, is still the foundation of behavior change." From relia1 at earthlink.net Wed Nov 16 09:55:41 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 16 Nov 2016 10:55:41 -0500 Subject: [Histonet] Thankfulness!! RELIA Histology Careers Bulletin - Exciting opportunities in CA, VA, WY, WI, TX, TN and CO Message-ID: <30cf01d24021$e3b19450$ab14bcf0$@earthlink.net> Hi Histonetters! How are you? Thanksgiving is just a little over a week away And I wanted share with you one of the things that I am most Thankful for. You! Thank you for reading my postings. I know you aren't looking for a job every time you open one and I know that your in box is precious real estate. I hope that you enjoy the little tidbits that I share from time to time. And remember if you refer someone to me and I place them you will earn a referral bonus. And of course if you are looking for a new opportunity I am always here to help! Sooo. As we gear up for the beginning of the holidays with Thanksgiving just a little over a week away; please allow me to share with you the list of my current openings. I have a number of really great opportunities. All of these positions are full time, permanent and offer excellent pay, great benefits and in some cases sign on bonuses and relocation assistance. The great thing about these opportunities is that if you are ready to move right away they are too and if you want to pursue a position with a start date after the holidays that works as well!! Here is a list of some of our current openings that I am excited about! Lead Histotech - San Diego, CA /Brand new lab!! Lead Histotech - Cheyenne, WY / Brand new lab!! IHC Tech Modesto, CA Histotech - Modesto, CA Histology Tech - Manitowoc, WI Grossing Tech - Austin, TX Histotech - Salem, VA entry level certified techs welcome to apply! Histotech- Glenwood, Springs, CO Pathologists' Assistant- Chattanooga, TN If you or anyone you know might be interested in any of these opportunities or would like help with a job search in another area of the USA please contact me. I can be reached at relia1 at earthlink.net or toll free at 866-607-3542 or on my cell/text 407-353-5070. Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From melissa at alliedsearchpartners.com Wed Nov 16 12:43:24 2016 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Wed, 16 Nov 2016 18:43:24 +0000 Subject: [Histonet] Permanent/Full Time IHC Support Specialist Job in Maryland- Message-ID: Hello Histonet, I have an opening for an experienced IHC Tech to take on a Support Specialist job opening. Must have IHC experience, understanding of IHC troubleshooting and maintenance of analyzers, and excellent customer service skills. Please contact me for details. Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 From jriggleman at globusmedical.com Wed Nov 16 14:11:37 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Wed, 16 Nov 2016 20:11:37 +0000 Subject: [Histonet] Polycut Microtome - MMA Thin Sections - Help! Message-ID: Hello, I am looking to use a Reichert-Jung Polycut S Microtome to cut MMA thin sections (5-10 microns). However the specimens I am cutting are very small. How do you go about gripping such small sections on such a microtome? I have tried using a dental mount to adhere the specimen to a blank MMA block - however the block or knife slipped and ruined the specimen. If anyone could offer tips/tricks/suggestions, that would be great! Thank you, Jessica Riggleman _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From mward at wakehealth.edu Wed Nov 16 14:32:59 2016 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Wed, 16 Nov 2016 20:32:59 +0000 Subject: [Histonet] New IHC instrument validation process Message-ID: How is everyone handling new instrument validation processes? We are looking at getting a new immunostainer. Beyond the varication process that the installers do what are our responsibilities from a lab stand point? We have a large menu of antibodies. Would it be sufficient to run one of each antibody to demonstrate that the new instrument compares to the old. The detection kits, retrieval solutions, antibodies, etc. would all remain the same. Thanks in advance for your help. Martha Ward Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157 From Debbie.Jerome at kadlecmed.org Wed Nov 16 14:35:49 2016 From: Debbie.Jerome at kadlecmed.org (Jerome, Debbie A) Date: Wed, 16 Nov 2016 12:35:49 -0800 Subject: [Histonet] ER PR Validation Message-ID: Hi All, Was looking for advise on ER PR validation - specifically, how many postive and negatives does CAP require? Or is there a CAP requirment? What have other labs done? Debbie Jerome, HT(ASCP) Histotechnician Laboratory Safety Officer Chemical Hygiene Officer KADLEC REGIONAL MEDICAL CENTER O 509-946-7686 F 509-946-2173 From EzanyaObeten at KentuckyOneHealth.org Wed Nov 16 14:44:59 2016 From: EzanyaObeten at KentuckyOneHealth.org (Obeten, Ezanya) Date: Wed, 16 Nov 2016 20:44:59 +0000 Subject: [Histonet] Lab Assistant Message-ID: Hi All, Was wondering what your job descriptions are for Lab Assistants in your laboratory. If you are able to add a pay range too, please do so. Thank you Ezanya Obeten, HTL (ASCP) Pathology Supervisor, Jewish Hospital, 200 Abraham Flexner Way, Louisville, Ky, 40202 Email: EzanyaObeten at KentuckyOneHealth.org P: 502-587-4933 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From LRaff at uropartners.com Wed Nov 16 15:20:41 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 16 Nov 2016 21:20:41 +0000 Subject: [Histonet] Lab Assistants Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF110BBF69@COLOEXCH01.uropartners.local> Our Anatomic Path lab assistants: 1) Accept cases from courier and accession them 2) Gross core biopsies (all our lab assistants have Bachelors Degrees and have been trained) 3) Cytology Prep 4) File slides and blocks 5) Fill supply orders for our clinician offices 6) Prepare some QA statistics Sorry can't release salary data here. ---------------------------------------------------------- Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 Current Blog: http://www.chicagonow.com/downsize-maybe/2016/11/forget-global-warming-we-have-global-weirding/ -----Original Message----- From: Obeten, Ezanya via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, November 16, 2016 2:45 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Lab Assistant Hi All, Was wondering what your job descriptions are for Lab Assistants in your laboratory. If you are able to add a pay range too, please do so. Thank you Ezanya Obeten, HTL (ASCP) Pathology Supervisor, Jewish Hospital, 200 Abraham Flexner Way, Louisville, Ky, 40202 Email: EzanyaObeten at KentuckyOneHealth.org P: 502-587-4933 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From shinwari_1 at yahoo.com Fri Nov 18 02:12:15 2016 From: shinwari_1 at yahoo.com (Nasir Abbas) Date: Fri, 18 Nov 2016 08:12:15 +0000 (UTC) Subject: [Histonet] Suggestions: Liver Pathology Book/Atlas References: <1438529359.2677539.1479456735345.ref@mail.yahoo.com> Message-ID: <1438529359.2677539.1479456735345@mail.yahoo.com> Hi Histo-FamilyI wonder if some one could suggest an updated and best Liver Pathology Book or Atlas with slight explanation, which covers all pathology assays. Or if some one already have for sale (used one), that will be preferred. Any suggestions please? Sincerely? Nasir Abbas ---PhD FellowGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan Avenue?Science Park?Guangzhou, 510530,?Guangdong?P.R.ChinaCell: +86-1312 8296 614 From natalie at ka-recruiting.com Fri Nov 18 12:12:18 2016 From: natalie at ka-recruiting.com (Natalie Ruch) Date: 18 Nov 2016 13:12:18 -0500 Subject: [Histonet] Exciting Full-Time MLT Opportunities Available Nationwide Message-ID: <122531837.1479492738323.JavaMail.cfservice@sl130app6> Dear Unknown, Happy Fall! I hope you are doing well. I wanted to reach out to you regarding the current MLT job opportunities that I am working on across the country. As a reminder, K.A. Recruiting is a leader in the healthcare recruiting industry and specializes in working with laboratory professionals across the country. All of our position are full-time and permanent. Our clients are eager to interview and hire before the end of the year and we have many great opportunities available! They also will relocate candidates permanently. ?I wanted to share our positions with you to see if you might be interested in learning more. If you are seeking new employment please send your most updated resume to Natalie at ka-recruiting.com and let me know the best time for us to get in touch. Finally, if you are not interested in a new opportunity. Feel free to forward my contact information along to anyone you might know seeking a new position. I look forward to hearing from you and have a wonderful holiday season! MLT Job Opportunities Available CA – MLT, day Shift IA - MT/ MLT IA - Med Tech or MLT - Night Shift, 4 10's IL - MT/ MLT (2nd shift) KY - MT/ MLT x 2 openings (f/t afternoon shift) MI - MT/MLT MT - MT/ MLT (day shift) ND - MT/MLT NE - MT/ MLT (Shifts vary, work four 9 hour shifts/ week) (x 2 openings) NE - MLT OH - MT/MLT x 2 openings (2nd & 3rd shift) PA - MT/ MLT WI - MT/ MLT (Thurs - Saturday night shift 6p-6a) TN - Hematology MT/ MLT x 2 openings (3rd shift opening) VA- Lab Technician, first shift M-F (can be MLT with Blood Bank experience) VA - Hematology x2 openings (night & Evening) & 1 MLT Hematology Sincerely, Natalie Ruch Healthcare Recruiting Manager K.A. Recruiting, Inc 10 Post Office Square 8th Floor South Boston, MA 02129 (P): 617.746.2660 (Please note new number) ext: 01 (F): 617.507.8009 This email was sent to histonet at lists.utsouthwestern.edu, by Natalie Ruch To remove your email address permanently from future mailings, please go to the following URL: http://email.bullhorn.com/wf/click?upn=9PHVqJROJZxwvA3zOhpnU0TB3U8uNa4Es-2FDIfIsgS4OF0Wzy-2BOQyPQKGbFmZz8mg-2BQzD075i8TlFtLeP-2FsEHq9jNcom-2F2t5BAmGZMfqIzW1SEHzvUtKJQU7-2FPi8hZRsTQhHEvQruCbfz9lws9pmjyqxfwfL4rJ9Q-2BtmPnmy0MC0-2FhNpn2PFj48J6kQjDF5t-2BvPb5RqhTwO0eOJF3QXsEQaS83H-2FwGn77Y42sKZMBqM3mQxovlaQ7mYPoKGbiyHSg2Vb8Sy9djmyfGPgOzqNZCP0dJ8QK0zD473Y6B0po0GMZm93aDPgVwSN4-2FzMy47lP-2F-2Bc3DqB7I9Jp35LVQEDQ7Q-3D-3D_4QNM6OU48aAU9YdlpNerABiNTwVWA-2FXLabFqU9mdUAFAD2LMMalOrBZjQh-2BpL7mOpOqQxFG-2FiO4xDwW-2B5PHa3eOsLE-2BzHUSuNctXESqMheW-2FkBiEO1p1JttLHrfQus3KAyIlxlwy9H2Xhn3njsEs-2BibqPkLovv5mkZhLRHXLv9V5Y2EbVcL3jI-2FjiGVogsrcKvFU6FlEFrWBKuga4BoFANCbYBpx3Hcc1nNNuMLkJRw-3D From Melissa.Kuhnla at chsli.org Mon Nov 21 09:43:59 2016 From: Melissa.Kuhnla at chsli.org (Kuhnla, Melissa) Date: Mon, 21 Nov 2016 15:43:59 +0000 Subject: [Histonet] (no subject) Message-ID: <4F2CE306C672504AAE5243945B1B4CD7184C1951@MVDCVM01XCN002.chsli.org> Hello, We looking to bring Kappa and Lambda by CISH on board here in my laboratory. We have Ventana Benchmark Ultra instruments. Can anyone running these tests let me know how long your staining protocol is? I am putting together a report on how this change will impact workflow and turn around times. Thank you all. Melissa Kuhnla Lead Medical Technologist for IHC and FISH testing Regional Laboratory Services Good Samaritan Hospital 631-609-2551 The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. From Erin.Martin at ucsf.edu Mon Nov 21 10:57:46 2016 From: Erin.Martin at ucsf.edu (Martin, Erin) Date: Mon, 21 Nov 2016 16:57:46 +0000 Subject: [Histonet] Leishmaniasis ctrl Message-ID: <24B7B291CC88D04AB663958E77A1F59DC91187@ex09.net.ucsf.edu> Hi all, Does anyone know of a source for a Leishmaniasis control block or control slides for IHC? Thanks in advance! Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. From tgenade at gmail.com Mon Nov 21 13:38:23 2016 From: tgenade at gmail.com (Tyrone Genade) Date: Mon, 21 Nov 2016 13:38:23 -0600 Subject: [Histonet] Maximow Method Message-ID: Hello, I was reading a book from the 60s on the anatomy of aging in man and animals and the author mentioned using a hematoxylin-eosin Y-Azure-II stain to show the lymphocytes. Some searching came up with the Maximow Method. The online protocol I found (for bone marrow): https://emsdiasum.com/microscopy/technical/datasheet/26252.aspx mentions the use of Zenker?s or Formalin. In another old book, Putt's Manual of Histochemical Staining Methods, the authors says 10% fomalin, Helly's or Zenker's fluid for fixation. I am definitely not going to start using Zenker's (I get enough grief from the H&S officer about picric acid). I normally use Davidson's fixative to fix and decalsify my fish (formaldehyde, acetic acid + ethanol). Anyone know if this staining method is compatible with Davidson's fixative? Would this eosin-Y solution be suitable: http://www.sigmaaldrich.com/catalog/product/sigma/ht110316?lang=en®ion=US for preparing the Eosin-Y Azure-II working solution? Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From shinwari_1 at yahoo.com Mon Nov 21 22:36:21 2016 From: shinwari_1 at yahoo.com (Nasir Abbas) Date: Tue, 22 Nov 2016 04:36:21 +0000 (UTC) Subject: [Histonet] Need Suggestions: IHC Slides and Floating Sections + 4% PFA Fixation References: <537107240.1775646.1479789381885.ref@mail.yahoo.com> Message-ID: <537107240.1775646.1479789381885@mail.yahoo.com> Hi All 1) How long vibratom liver sections when cut in ice cold PBS and then stored at 4C in PBS could be used??2) How long paraffin embedded liver tissues after dewaxing and antigen retrieval stored in PBS at 4C could be used? 3) Whether liver tissues fixed in 4% PFA for more than a month would have any affect on antigen integrity?when antigen of interests (CD68, F4/80, Ly6g, CD4 and CD8) are low in abundance (different time points from 15min to 96hrs of injury)? Has any body encountered such problem before? If such was the case with you, how would have you tackle it? and finally,3) Any body could suggest liver pathology atlas with normal to special stain please? For your opinions and suggestions, thanks in advance. Sincerely?Nasir Abbas ---PhD FellowGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan Avenue?Science Park?Guangzhou, 510530,?Guangdong?P.R.ChinaCell: +86-1312 8296 614 From rjbuesa at yahoo.com Tue Nov 22 11:08:44 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 22 Nov 2016 17:08:44 +0000 (UTC) Subject: [Histonet] Maximow Method In-Reply-To: References: Message-ID: <517096028.2202826.1479834524867@mail.yahoo.com> Tyrone:A.A.Maximow's Azur-eosin, etc staining produces wonderful results but this, and many very old procedures, essentially rest on the use of mercury salts which produce special chemical compounds with tissue components.Any, and I mean any, deviation from the original procedure will not produce the same results as those expected from the original recipes.You can try and you will get a staining but without the crisp and delightful colorations obtained with the original recipe containing mercury. The same goes for the Harris hematroxylin that now is manufactured and sold without mercury oxide and is still? named Harris, when really it is not. The results are similar, but not exactly as those obtained with the original recipe.So, in my humble opinion, you can substitute whatever you want from an original recipe, but please do not be surprised if the results do not "live" to your expectations or the original description.Ren?? On Monday, November 21, 2016 2:40 PM, Tyrone Genade via Histonet wrote: Hello, I was reading a book from the 60s on the anatomy of aging in man and animals and the author mentioned using a hematoxylin-eosin Y-Azure-II stain to show the lymphocytes. Some searching came up with the Maximow Method. The online protocol I found (for bone marrow): https://emsdiasum.com/microscopy/technical/datasheet/26252.aspx mentions the use of Zenker?s or Formalin. In another old book, Putt's Manual of Histochemical Staining Methods, the authors says 10% fomalin, Helly's or Zenker's fluid for fixation. I am definitely not going to start using Zenker's (I get enough grief from the H&S officer about picric acid). I normally use Davidson's fixative to fix and decalsify my fish (formaldehyde, acetic acid + ethanol). Anyone know if this staining method is compatible with Davidson's fixative? Would this eosin-Y solution be suitable: http://www.sigmaaldrich.com/catalog/product/sigma/ht110316?lang=en?ion=US for preparing the Eosin-Y Azure-II working solution? Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin at yumaregional.org Tue Nov 22 11:36:20 2016 From: JEllin at yumaregional.org (Jesus Ellin) Date: Tue, 22 Nov 2016 17:36:20 +0000 Subject: [Histonet] Personel Message-ID: So I know I am going to open Pandoras box,, but have people been paying attention to the Personal requirements from CAP. I called the CAP and asked them about the criteria concerning Moderate or High complexity testing, after discussing with them the situations, IF you have a tech that is Licensed and Also has a QIHC, but does not minimum requirement Defined by CLIA in education ,, they CAN NOT do any QA/OC of IHC and antibody work up,, as IHC is defined as High complexity testing. I also asked about the test systems. The grandfather clause is only good for test systems that occurred for those time periods. For instance if CLIA defined the test system after those dates of 1997,, then they are not included and the person cannot perform test and technology created after those dates, since the testing was not in place during the grandfather clause time. In a nut shell meaning if the IHC staining and antibody was developed after those dates,, you are not covered by the grandfather clause to do the testing ,, can some help clear this up,, So any help on this matter will do ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From Timothy.Morken at ucsf.edu Tue Nov 22 12:16:32 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 22 Nov 2016 18:16:32 +0000 Subject: [Histonet] Personel In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu> Jesus, that is very interesting information. Does anyone know of any CAP accreditation documents that state explicitly that IHC slide staining is high complexity? I have not seen any. If anyone has those documents I'd like to see them. The only reference from CAP about that classification I have seen was in a Q&A session transcript from a CAP webinar on competency testing. The webinar had no information about IHC and complexity. However, a presenter answering a question about whether IHC staining at the bench is a high complexity "test," did state that IHC staining is high complexity so the techs doing the staining must have competency testing. Very strange! That's not to say I don't think IHC is high complexity - I do, and so is every other test in histology. But under CLIA the testing personnel is the pathologist, not the bench tech. CAP can deem IHC bench testing as high complexity if it wishes (CLIA is a baseline and deemed accrediting agencies, and institutions, can have stricter requirements). But it seems the only way anyone can find out if CAP classifies IHC as high complexity is to call them and ask. Your comment about new technology is interesting. In a modern scenario, which tech is the person who is "staining" the slide? And which of these is the "high complexity" part of the process? 1) person collating slides to stain 2) Person who programs the stainer 3) Person who dilutes the antibodies (still done!) 4) person who loads reagents on the stainer 5) person who loads the slides on the stainer 6) person who starts the stainer 7) person who unloads the slides from the stainer 8) person who labels and distributes the slides. 9) Person who checks QC slides (BTW, not a "test,"). In our lab these tasks are traded off by many different people throughout the day How about the person doing the validation of the stain? They are not doing a "test" but they are making the test possible to do. Just some questions to ponder over the holidays! Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Jesus Ellin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 22, 2016 9:36 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Personel So I know I am going to open Pandoras box,, but have people been paying attention to the Personal requirements from CAP. I called the CAP and asked them about the criteria concerning Moderate or High complexity testing, after discussing with them the situations, IF you have a tech that is Licensed and Also has a QIHC, but does not minimum requirement Defined by CLIA in education ,, they CAN NOT do any QA/OC of IHC and antibody work up,, as IHC is defined as High complexity testing. I also asked about the test systems. The grandfather clause is only good for test systems that occurred for those time periods. For instance if CLIA defined the test system after those dates of 1997,, then they are not included and the person cannot perform test and technology created after those dates, since the testing was not in place during the grandfather clause time. In a nut shell meaning if the IHC staining and antibody was developed after those dates,, you are not covered by the grandfather clause to do the testing ,, can some help clear this up,, So any help on this matter will do ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin at yumaregional.org Tue Nov 22 12:37:21 2016 From: JEllin at yumaregional.org (Jesus Ellin) Date: Tue, 22 Nov 2016 18:37:21 +0000 Subject: [Histonet] Personel In-Reply-To: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu> Message-ID: I am going to attach the information where you can find what is high complexity testing as defined by CLIA,, also CAP defines the QA/QC of this process to be the high complexity ,, also the antibody workup,, here is the website where you can get this information from. Again if you look at the CAP regs its states there the QA/QC as high complexity,, We can cut pull controls, place on machine and run,, but we can not Qa/QC reaction,, weird huh. On another note the inspector stated that the grandfather clause is good for those testing methologies and test that were pre 1997,, so if still doing same testing after 1997 then good to go,, if test have changed for instance predictive markers, testing kits that are defined by the link I sent you ,, then they are not eligible unless they meet those requirments. Your thoughts Jesus Ellin http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfCLIA/Search.cfm -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Tuesday, November 22, 2016 11:17 AM To: Jesus Ellin Cc: Histonet Subject: RE: Personel Jesus, that is very interesting information. Does anyone know of any CAP accreditation documents that state explicitly that IHC slide staining is high complexity? I have not seen any. If anyone has those documents I'd like to see them. The only reference from CAP about that classification I have seen was in a Q&A session transcript from a CAP webinar on competency testing. The webinar had no information about IHC and complexity. However, a presenter answering a question about whether IHC staining at the bench is a high complexity "test," did state that IHC staining is high complexity so the techs doing the staining must have competency testing. Very strange! That's not to say I don't think IHC is high complexity - I do, and so is every other test in histology. But under CLIA the testing personnel is the pathologist, not the bench tech. CAP can deem IHC bench testing as high complexity if it wishes (CLIA is a baseline and deemed accrediting agencies, and institutions, can have stricter requirements). But it seems the only way anyone can find out if CAP classifies IHC as high complexity is to call them and ask. Your comment about new technology is interesting. In a modern scenario, which tech is the person who is "staining" the slide? And which of these is the "high complexity" part of the process? 1) person collating slides to stain 2) Person who programs the stainer 3) Person who dilutes the antibodies (still done!) 4) person who loads reagents on the stainer 5) person who loads the slides on the stainer 6) person who starts the stainer 7) person who unloads the slides from the stainer 8) person who labels and distributes the slides. 9) Person who checks QC slides (BTW, not a "test,"). In our lab these tasks are traded off by many different people throughout the day How about the person doing the validation of the stain? They are not doing a "test" but they are making the test possible to do. Just some questions to ponder over the holidays! Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Jesus Ellin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 22, 2016 9:36 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Personel So I know I am going to open Pandoras box,, but have people been paying attention to the Personal requirements from CAP. I called the CAP and asked them about the criteria concerning Moderate or High complexity testing, after discussing with them the situations, IF you have a tech that is Licensed and Also has a QIHC, but does not minimum requirement Defined by CLIA in education ,, they CAN NOT do any QA/OC of IHC and antibody work up,, as IHC is defined as High complexity testing. I also asked about the test systems. The grandfather clause is only good for test systems that occurred for those time periods. For instance if CLIA defined the test system after those dates of 1997,, then they are not included and the person cannot perform test and technology created after those dates, since the testing was not in place during the grandfather clause time. In a nut shell meaning if the IHC staining and antibody was developed after those dates,, you are not covered by the grandfather clause to do the testing ,, can some help clear this up,, So any help on this matter will do ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From tbraud at holyredeemer.com Tue Nov 22 12:42:12 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 22 Nov 2016 18:42:12 +0000 Subject: [Histonet] IHC Personel Message-ID: <48E053DDF6CE074DB6A7414BA05403F81113DC@HRHEX03-HOS.holyredeemer.local> This should help...the IHC "test" is the pathologists' INTERPRETATION of IHC stain. The stain procedure, validation of protocols and controls, and lot to lot validation must be signed off by a pathologist. Any tech that has demonstrated competency in performing the procedure, can perform IHC staining. Any tech can also perform an antibody work-up, provided the "results" are signed off by a pathologist. Easy-peasy Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 -----Original Message----- 4. Personel (Jesus Ellin) ---------------------------------------------------------------------- Message: 4 Date: Tue, 22 Nov 2016 17:36:20 +0000 From: Jesus Ellin Subject: [Histonet] Personel So I know I am going to open Pandoras box,, but have people been paying attention to the Personal requirements from CAP. I called the CAP and asked them about the criteria concerning Moderate or High complexity testing, after discussing with them the situations, IF you have a tech that is Licensed and Also has a QIHC, but does not minimum requirement Defined by CLIA in education ,, they CAN NOT do any QA/OC of IHC and antibody work up,, as IHC is defined as High complexity testing. I also asked about the test systems. The grandfather clause is only good for test systems that occurred for those time periods. For instance if CLIA defined the test system after those dates of 1997,, then they are not included and the person cannot perform test and technology created after those dates, since the testing was not in place during the grandfather clause time. In a nut shell meaning if the IHC staining and antibody was developed after those dates,, you are not covered by the grandfather clause to do the testing ,, can some help clear this up,, So any help on this matter will do From Timothy.Morken at ucsf.edu Tue Nov 22 13:41:14 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 22 Nov 2016 19:41:14 +0000 Subject: [Histonet] Personel In-Reply-To: <9C8F910F72893643B3C3793C3D67132B67FF5FB0@PATHOLOGYSERVER.pathologyarts.local> References: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu> <9C8F910F72893643B3C3793C3D67132B67FF5FB0@PATHOLOGYSERVER.pathologyarts.local> Message-ID: <761E2B5697F795489C8710BCC72141FF88E2803D@ex07.net.ucsf.edu> Curt, Yes, but that is the test, not the personnel, and specifically applies to the interpretation of the test, not the running of the machine. In AP the "testing personnel" is the pathologist, not the tech running the machine. Only the pathologist interprets the stains and reports a result. They also have final signoff on any QC. I have never seen an explicit explanation from CAP about how the CLIA regs fit with histology. CLIA was written for the clinical lab where the MT's report results directly. CLIA considers all histology personnel as "Processing Personnel" not testing personnel. CAP has taken it up a notch, which they are allowed to do, but they have not provided any explicit guidance as to how it applies in histology. For instance, why is IHC high complexity but special stains are not? They are similar in complexity of processing. I give workshops on competency testing in histology. This question is the number one question. Where does high complexity apply? All I want is for CAP to produce a document explaining their rational so people don't have to call them to get answers, or, god forbid, depend on a CAP inspector for the answer, most of which are contradictory from one inspector to another. Tim -----Original Message----- From: Curt [mailto:c.tague at Pathologyarts.com] Sent: Tuesday, November 22, 2016 10:43 AM To: Morken, Timothy; Jesus Ellin Cc: histonet at lists.utsouthwestern.edu Subject: RE: Personel I recently had this same discussion with Jesus, please follow this link he gave me: http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfCLIA/Search.cfm At the top, enter "Test System / Manufacturer" for example, we enter Ventana, it will list all tests that qualify as High Complexity. There are 4-5 different tab/pages... even operating something as basic as a DAB detection kit appears to qualify as High Complexity...if you enter Leica, then you get much less but the detection kit is listed as High Complexity still so it would serve to reason that someone not qualified for high complexity testing cannot even load slides... maybe they can if a qualified person is the one prepping the machine, loading detection kits and AB's??? High Complexity testing personnel requirements per CAP: 1. MD or DO with a current medical license?; OR 2. Doctoral degree in clinical laboratory science, chemical, physical or biological science; OR 3. Master's degree in medical technology, clinical laboratory, chemical, physical, or biological science; OR 4. Bachelor's degree in medical technology, clinical laboratory, chemical, physical or biological; OR 5. Associate degree in chemical, physical or biological science or medical laboratory or equivalent education and training (refer to 42CFR493.1489(b) for details on required courses and training); OR 6. Individuals performing high complexity testing on or before April 24, 1995 with a high school diploma or equivalent with documented training may continue to perform testing only on those tests for which training was documented prior to September 1, 1997 (refer to CLIA regulation 42CFR493.1489(b) for details on required training) Curt -----Original Message----- From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 22, 2016 10:17 AM To: Jesus Ellin Cc: Histonet Subject: Re: [Histonet] Personel Jesus, that is very interesting information. Does anyone know of any CAP accreditation documents that state explicitly that IHC slide staining is high complexity? I have not seen any. If anyone has those documents I'd like to see them. The only reference from CAP about that classification I have seen was in a Q&A session transcript from a CAP webinar on competency testing. The webinar had no information about IHC and complexity. However, a presenter answering a question about whether IHC staining at the bench is a high complexity "test," did state that IHC staining is high complexity so the techs doing the staining must have competency testing. Very strange! That's not to say I don't think IHC is high complexity - I do, and so is every other test in histology. But under CLIA the testing personnel is the pathologist, not the bench tech. CAP can deem IHC bench testing as high complexity if it wishes (CLIA is a baseline and deemed accrediting agencies, and institutions, can have stricter requirements). But it seems the only way anyone can find out if CAP classifies IHC as high complexity is to call them and ask. Your comment about new technology is interesting. In a modern scenario, which tech is the person who is "staining" the slide? And which of these is the "high complexity" part of the process? 1) person collating slides to stain 2) Person who programs the stainer 3) Person who dilutes the antibodies (still done!) 4) person who loads reagents on the stainer 5) person who loads the slides on the stainer 6) person who starts the stainer 7) person who unloads the slides from the stainer 8) person who labels and distributes the slides. 9) Person who checks QC slides (BTW, not a "test,"). In our lab these tasks are traded off by many different people throughout the day How about the person doing the validation of the stain? They are not doing a "test" but they are making the test possible to do. Just some questions to ponder over the holidays! Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Jesus Ellin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 22, 2016 9:36 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Personel So I know I am going to open Pandoras box,, but have people been paying attention to the Personal requirements from CAP. I called the CAP and asked them about the criteria concerning Moderate or High complexity testing, after discussing with them the situations, IF you have a tech that is Licensed and Also has a QIHC, but does not minimum requirement Defined by CLIA in education ,, they CAN NOT do any QA/OC of IHC and antibody work up,, as IHC is defined as High complexity testing. I also asked about the test systems. The grandfather clause is only good for test systems that occurred for those time periods. For instance if CLIA defined the test system after those dates of 1997,, then they are not included and the person cannot perform test and technology created after those dates, since the testing was not in place during the grandfather clause time. In a nut shell meaning if the IHC staining and antibody was developed after those dates,, you are not covered by the grandfather clause to do the testing ,, can some help clear this up,, So any help on this matter will do ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SteveM at mcclainlab.com Tue Nov 22 13:49:16 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Tue, 22 Nov 2016 19:49:16 +0000 Subject: [Histonet] Histonet Digest, Vol 156, Issue 20a hematoxylin-eosin Y-Azure-II stain In-Reply-To: References: Message-ID: <3EEC43FC-43DC-4FEB-A63C-95EA2C7CBD17@mcclainlab.com> hematoxylin-eosin Y-Azure-II stain Agree w Ren. But try it anyway. To bring out the azure, you may wish to post fix. Steve A. McClain, MD > On Nov 22, 2016, at 13:29, "histonet-request at lists.utsouthwestern.edu" wrote: > > a hematoxylin-eosin Y-Azure-II stain From Timothy.Morken at ucsf.edu Tue Nov 22 14:11:48 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 22 Nov 2016 20:11:48 +0000 Subject: [Histonet] Personel In-Reply-To: <9C8F910F72893643B3C3793C3D67132B67FF60C8@PATHOLOGYSERVER.pathologyarts.local> References: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu> <9C8F910F72893643B3C3793C3D67132B67FF5FB0@PATHOLOGYSERVER.pathologyarts.local> <761E2B5697F795489C8710BCC72141FF88E2803D@ex07.net.ucsf.edu> <9C8F910F72893643B3C3793C3D67132B67FF60C8@PATHOLOGYSERVER.pathologyarts.local> Message-ID: <761E2B5697F795489C8710BCC72141FF88E28087@ex07.net.ucsf.edu> Curt, Well, that is the situation under the CLIA regs, but a deemed accrediting agency, like CAP or JC, can implement stricter requirements. An institution can do so as well (a medical center can require all its histology techs to qualify for high complexity testing if it wants). The ONLY issue I have with CAP is that their "requirement" that techs running IHC staining (manual or machines) must qualify under high complexity education requirements is not explicitly stated anywhere in their documents, at least that I have seen. There is no explicit determination that IHC staining is itself high complexity, and that the staining personnel are "testing personnel." If I work through the CLIA and FDA algorithm to determine test complexity, then I can show that ALL histology is high complexity. So why is only IHC considered high complexity for personnel requirements/competency testing? I don't know. I have a feeling it is because there are very critical tests done that affect treatment so we do want the best people doing this work. But I have not seen any explanation to that effect. And we have similar tests in special stains. Why are they not "high complexity?" I would like to see CAP produce a definitive document explaining their rational. That's it. For some reason they don't want to do that. Their various documents and charts are not much help because they do not define a TEST for histopathology. They mention "test systems" and "testing personnel" but do not really define what is included in that system. For instance, does an IHC "test system" include sectioning? Storage of slides? At what point does IHC staining "start" and "end?" We are under Joint Commission and they do not consider histology "processing" to be a "test" and they consider any slide staining as "processing." So they do not require competency testing for histology personnel (We do it anyway because it is a good thing to do). Tim -----Original Message----- From: Curt [mailto:c.tague at Pathologyarts.com] Sent: Tuesday, November 22, 2016 11:51 AM To: Morken, Timothy; Jesus Ellin Cc: histonet at lists.utsouthwestern.edu Subject: RE: Personel That is very helpful. The test is the rendering of an interpretation or diagnosis, not the mere operation of the machinery, correct? Curt -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Tuesday, November 22, 2016 11:41 AM To: Curt; Jesus Ellin Cc: histonet at lists.utsouthwestern.edu Subject: RE: Personel Curt, Yes, but that is the test, not the personnel, and specifically applies to the interpretation of the test, not the running of the machine. In AP the "testing personnel" is the pathologist, not the tech running the machine. Only the pathologist interprets the stains and reports a result. They also have final signoff on any QC. I have never seen an explicit explanation from CAP about how the CLIA regs fit with histology. CLIA was written for the clinical lab where the MT's report results directly. CLIA considers all histology personnel as "Processing Personnel" not testing personnel. CAP has taken it up a notch, which they are allowed to do, but they have not provided any explicit guidance as to how it applies in histology. For instance, why is IHC high complexity but special stains are not? They are similar in complexity of processing. I give workshops on competency testing in histology. This question is the number one question. Where does high complexity apply? All I want is for CAP to produce a document explaining their rational so people don't have to call them to get answers, or, god forbid, depend on a CAP inspector for the answer, most of which are contradictory from one inspector to another. Tim -----Original Message----- From: Curt [mailto:c.tague at Pathologyarts.com] Sent: Tuesday, November 22, 2016 10:43 AM To: Morken, Timothy; Jesus Ellin Cc: histonet at lists.utsouthwestern.edu Subject: RE: Personel I recently had this same discussion with Jesus, please follow this link he gave me: http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfCLIA/Search.cfm At the top, enter "Test System / Manufacturer" for example, we enter Ventana, it will list all tests that qualify as High Complexity. There are 4-5 different tab/pages... even operating something as basic as a DAB detection kit appears to qualify as High Complexity...if you enter Leica, then you get much less but the detection kit is listed as High Complexity still so it would serve to reason that someone not qualified for high complexity testing cannot even load slides... maybe they can if a qualified person is the one prepping the machine, loading detection kits and AB's??? High Complexity testing personnel requirements per CAP: 1. MD or DO with a current medical license?; OR 2. Doctoral degree in clinical laboratory science, chemical, physical or biological science; OR 3. Master's degree in medical technology, clinical laboratory, chemical, physical, or biological science; OR 4. Bachelor's degree in medical technology, clinical laboratory, chemical, physical or biological; OR 5. Associate degree in chemical, physical or biological science or medical laboratory or equivalent education and training (refer to 42CFR493.1489(b) for details on required courses and training); OR 6. Individuals performing high complexity testing on or before April 24, 1995 with a high school diploma or equivalent with documented training may continue to perform testing only on those tests for which training was documented prior to September 1, 1997 (refer to CLIA regulation 42CFR493.1489(b) for details on required training) Curt -----Original Message----- From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 22, 2016 10:17 AM To: Jesus Ellin Cc: Histonet Subject: Re: [Histonet] Personel Jesus, that is very interesting information. Does anyone know of any CAP accreditation documents that state explicitly that IHC slide staining is high complexity? I have not seen any. If anyone has those documents I'd like to see them. The only reference from CAP about that classification I have seen was in a Q&A session transcript from a CAP webinar on competency testing. The webinar had no information about IHC and complexity. However, a presenter answering a question about whether IHC staining at the bench is a high complexity "test," did state that IHC staining is high complexity so the techs doing the staining must have competency testing. Very strange! That's not to say I don't think IHC is high complexity - I do, and so is every other test in histology. But under CLIA the testing personnel is the pathologist, not the bench tech. CAP can deem IHC bench testing as high complexity if it wishes (CLIA is a baseline and deemed accrediting agencies, and institutions, can have stricter requirements). But it seems the only way anyone can find out if CAP classifies IHC as high complexity is to call them and ask. Your comment about new technology is interesting. In a modern scenario, which tech is the person who is "staining" the slide? And which of these is the "high complexity" part of the process? 1) person collating slides to stain 2) Person who programs the stainer 3) Person who dilutes the antibodies (still done!) 4) person who loads reagents on the stainer 5) person who loads the slides on the stainer 6) person who starts the stainer 7) person who unloads the slides from the stainer 8) person who labels and distributes the slides. 9) Person who checks QC slides (BTW, not a "test,"). In our lab these tasks are traded off by many different people throughout the day How about the person doing the validation of the stain? They are not doing a "test" but they are making the test possible to do. Just some questions to ponder over the holidays! Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Jesus Ellin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 22, 2016 9:36 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Personel So I know I am going to open Pandoras box,, but have people been paying attention to the Personal requirements from CAP. I called the CAP and asked them about the criteria concerning Moderate or High complexity testing, after discussing with them the situations, IF you have a tech that is Licensed and Also has a QIHC, but does not minimum requirement Defined by CLIA in education ,, they CAN NOT do any QA/OC of IHC and antibody work up,, as IHC is defined as High complexity testing. I also asked about the test systems. The grandfather clause is only good for test systems that occurred for those time periods. For instance if CLIA defined the test system after those dates of 1997,, then they are not included and the person cannot perform test and technology created after those dates, since the testing was not in place during the grandfather clause time. In a nut shell meaning if the IHC staining and antibody was developed after those dates,, you are not covered by the grandfather clause to do the testing ,, can some help clear this up,, So any help on this matter will do ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From epeters2 at gmu.edu Tue Nov 22 16:24:11 2016 From: epeters2 at gmu.edu (Esther C Peters) Date: Tue, 22 Nov 2016 22:24:11 +0000 Subject: [Histonet] Weigert's hematoxylin for IHC Message-ID: Could someone advise me on whether Weigert's iron hematoxylin can be used as the counterstain for nuclei in IHC? We need to avoid using Harris's hematoxylin because that will stain mucocytes in the coral tissues we are working with. If it can be used, would that be after all antibody steps have been done? Thank you! Esther Peters Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epeters2 at gmu.edu http://esp.gmu.edu From mills at 3scan.com Tue Nov 22 17:12:03 2016 From: mills at 3scan.com (Caroline Miller) Date: Tue, 22 Nov 2016 15:12:03 -0800 Subject: [Histonet] Weigert's hematoxylin for IHC In-Reply-To: References: Message-ID: I would caution using Weigerts as a counterstain for IHC because it is pretty dark in color and could be confused with the brown of the staining you are looking for in your IHC. Have you tried Mayer's? That often provides a pleasing stain with minimal effort. You would counterstain after the DAB development steps, prior to mounting the slides with coverslips. I have never seen coral staining though, so this is only a guess from what works in regular mammalian tissues, good luck! yours, mills On Tue, Nov 22, 2016 at 2:24 PM, Esther C Peters via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Could someone advise me on whether Weigert's iron hematoxylin can be used > as the counterstain for nuclei in IHC? We need to avoid using Harris's > hematoxylin because that will stain mucocytes in the coral tissues we are > working with. If it can be used, would that be after all antibody steps > have been done? Thank you! > > > Esther Peters > > > Esther C. Peters, Ph.D. > Term Associate Professor > Environmental Science & Policy > George Mason University > 4400 University Drive, MS 5F2 > Fairfax, VA 22030-4444 > Office: David King Hall, Room 3050 > Phone: 703-993-3462 > Fax: 703-993-1066 > e-mail: epeters2 at gmu.edu > xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPP > KrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu>http://esp.gmu.edu > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From c.tague at Pathologyarts.com Tue Nov 22 12:42:53 2016 From: c.tague at Pathologyarts.com (Curt) Date: Tue, 22 Nov 2016 18:42:53 +0000 Subject: [Histonet] Personel In-Reply-To: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu> Message-ID: <9C8F910F72893643B3C3793C3D67132B67FF5FB0@PATHOLOGYSERVER.pathologyarts.local> I recently had this same discussion with Jesus, please follow this link he gave me: http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfCLIA/Search.cfm At the top, enter "Test System / Manufacturer" for example, we enter Ventana, it will list all tests that qualify as High Complexity. There are 4-5 different tab/pages... even operating something as basic as a DAB detection kit appears to qualify as High Complexity...if you enter Leica, then you get much less but the detection kit is listed as High Complexity still so it would serve to reason that someone not qualified for high complexity testing cannot even load slides... maybe they can if a qualified person is the one prepping the machine, loading detection kits and AB's??? High Complexity testing personnel requirements per CAP: 1. MD or DO with a current medical license?; OR 2. Doctoral degree in clinical laboratory science, chemical, physical or biological science; OR 3. Master's degree in medical technology, clinical laboratory, chemical, physical, or biological science; OR 4. Bachelor's degree in medical technology, clinical laboratory, chemical, physical or biological; OR 5. Associate degree in chemical, physical or biological science or medical laboratory or equivalent education and training (refer to 42CFR493.1489(b) for details on required courses and training); OR 6. Individuals performing high complexity testing on or before April 24, 1995 with a high school diploma or equivalent with documented training may continue to perform testing only on those tests for which training was documented prior to September 1, 1997 (refer to CLIA regulation 42CFR493.1489(b) for details on required training) Curt -----Original Message----- From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 22, 2016 10:17 AM To: Jesus Ellin Cc: Histonet Subject: Re: [Histonet] Personel Jesus, that is very interesting information. Does anyone know of any CAP accreditation documents that state explicitly that IHC slide staining is high complexity? I have not seen any. If anyone has those documents I'd like to see them. The only reference from CAP about that classification I have seen was in a Q&A session transcript from a CAP webinar on competency testing. The webinar had no information about IHC and complexity. However, a presenter answering a question about whether IHC staining at the bench is a high complexity "test," did state that IHC staining is high complexity so the techs doing the staining must have competency testing. Very strange! That's not to say I don't think IHC is high complexity - I do, and so is every other test in histology. But under CLIA the testing personnel is the pathologist, not the bench tech. CAP can deem IHC bench testing as high complexity if it wishes (CLIA is a baseline and deemed accrediting agencies, and institutions, can have stricter requirements). But it seems the only way anyone can find out if CAP classifies IHC as high complexity is to call them and ask. Your comment about new technology is interesting. In a modern scenario, which tech is the person who is "staining" the slide? And which of these is the "high complexity" part of the process? 1) person collating slides to stain 2) Person who programs the stainer 3) Person who dilutes the antibodies (still done!) 4) person who loads reagents on the stainer 5) person who loads the slides on the stainer 6) person who starts the stainer 7) person who unloads the slides from the stainer 8) person who labels and distributes the slides. 9) Person who checks QC slides (BTW, not a "test,"). In our lab these tasks are traded off by many different people throughout the day How about the person doing the validation of the stain? They are not doing a "test" but they are making the test possible to do. Just some questions to ponder over the holidays! Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Jesus Ellin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 22, 2016 9:36 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Personel So I know I am going to open Pandoras box,, but have people been paying attention to the Personal requirements from CAP. I called the CAP and asked them about the criteria concerning Moderate or High complexity testing, after discussing with them the situations, IF you have a tech that is Licensed and Also has a QIHC, but does not minimum requirement Defined by CLIA in education ,, they CAN NOT do any QA/OC of IHC and antibody work up,, as IHC is defined as High complexity testing. I also asked about the test systems. The grandfather clause is only good for test systems that occurred for those time periods. For instance if CLIA defined the test system after those dates of 1997,, then they are not included and the person cannot perform test and technology created after those dates, since the testing was not in place during the grandfather clause time. In a nut shell meaning if the IHC staining and antibody was developed after those dates,, you are not covered by the grandfather clause to do the testing ,, can some help clear this up,, So any help on this matter will do ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague at Pathologyarts.com Tue Nov 22 13:51:19 2016 From: c.tague at Pathologyarts.com (Curt) Date: Tue, 22 Nov 2016 19:51:19 +0000 Subject: [Histonet] Personel In-Reply-To: <761E2B5697F795489C8710BCC72141FF88E2803D@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu> <9C8F910F72893643B3C3793C3D67132B67FF5FB0@PATHOLOGYSERVER.pathologyarts.local> <761E2B5697F795489C8710BCC72141FF88E2803D@ex07.net.ucsf.edu> Message-ID: <9C8F910F72893643B3C3793C3D67132B67FF60C8@PATHOLOGYSERVER.pathologyarts.local> That is very helpful. The test is the rendering of an interpretation or diagnosis, not the mere operation of the machinery, correct? Curt -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Tuesday, November 22, 2016 11:41 AM To: Curt; Jesus Ellin Cc: histonet at lists.utsouthwestern.edu Subject: RE: Personel Curt, Yes, but that is the test, not the personnel, and specifically applies to the interpretation of the test, not the running of the machine. In AP the "testing personnel" is the pathologist, not the tech running the machine. Only the pathologist interprets the stains and reports a result. They also have final signoff on any QC. I have never seen an explicit explanation from CAP about how the CLIA regs fit with histology. CLIA was written for the clinical lab where the MT's report results directly. CLIA considers all histology personnel as "Processing Personnel" not testing personnel. CAP has taken it up a notch, which they are allowed to do, but they have not provided any explicit guidance as to how it applies in histology. For instance, why is IHC high complexity but special stains are not? They are similar in complexity of processing. I give workshops on competency testing in histology. This question is the number one question. Where does high complexity apply? All I want is for CAP to produce a document explaining their rational so people don't have to call them to get answers, or, god forbid, depend on a CAP inspector for the answer, most of which are contradictory from one inspector to another. Tim -----Original Message----- From: Curt [mailto:c.tague at Pathologyarts.com] Sent: Tuesday, November 22, 2016 10:43 AM To: Morken, Timothy; Jesus Ellin Cc: histonet at lists.utsouthwestern.edu Subject: RE: Personel I recently had this same discussion with Jesus, please follow this link he gave me: http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfCLIA/Search.cfm At the top, enter "Test System / Manufacturer" for example, we enter Ventana, it will list all tests that qualify as High Complexity. There are 4-5 different tab/pages... even operating something as basic as a DAB detection kit appears to qualify as High Complexity...if you enter Leica, then you get much less but the detection kit is listed as High Complexity still so it would serve to reason that someone not qualified for high complexity testing cannot even load slides... maybe they can if a qualified person is the one prepping the machine, loading detection kits and AB's??? High Complexity testing personnel requirements per CAP: 1. MD or DO with a current medical license?; OR 2. Doctoral degree in clinical laboratory science, chemical, physical or biological science; OR 3. Master's degree in medical technology, clinical laboratory, chemical, physical, or biological science; OR 4. Bachelor's degree in medical technology, clinical laboratory, chemical, physical or biological; OR 5. Associate degree in chemical, physical or biological science or medical laboratory or equivalent education and training (refer to 42CFR493.1489(b) for details on required courses and training); OR 6. Individuals performing high complexity testing on or before April 24, 1995 with a high school diploma or equivalent with documented training may continue to perform testing only on those tests for which training was documented prior to September 1, 1997 (refer to CLIA regulation 42CFR493.1489(b) for details on required training) Curt -----Original Message----- From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 22, 2016 10:17 AM To: Jesus Ellin Cc: Histonet Subject: Re: [Histonet] Personel Jesus, that is very interesting information. Does anyone know of any CAP accreditation documents that state explicitly that IHC slide staining is high complexity? I have not seen any. If anyone has those documents I'd like to see them. The only reference from CAP about that classification I have seen was in a Q&A session transcript from a CAP webinar on competency testing. The webinar had no information about IHC and complexity. However, a presenter answering a question about whether IHC staining at the bench is a high complexity "test," did state that IHC staining is high complexity so the techs doing the staining must have competency testing. Very strange! That's not to say I don't think IHC is high complexity - I do, and so is every other test in histology. But under CLIA the testing personnel is the pathologist, not the bench tech. CAP can deem IHC bench testing as high complexity if it wishes (CLIA is a baseline and deemed accrediting agencies, and institutions, can have stricter requirements). But it seems the only way anyone can find out if CAP classifies IHC as high complexity is to call them and ask. Your comment about new technology is interesting. In a modern scenario, which tech is the person who is "staining" the slide? And which of these is the "high complexity" part of the process? 1) person collating slides to stain 2) Person who programs the stainer 3) Person who dilutes the antibodies (still done!) 4) person who loads reagents on the stainer 5) person who loads the slides on the stainer 6) person who starts the stainer 7) person who unloads the slides from the stainer 8) person who labels and distributes the slides. 9) Person who checks QC slides (BTW, not a "test,"). In our lab these tasks are traded off by many different people throughout the day How about the person doing the validation of the stain? They are not doing a "test" but they are making the test possible to do. Just some questions to ponder over the holidays! Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Jesus Ellin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 22, 2016 9:36 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Personel So I know I am going to open Pandoras box,, but have people been paying attention to the Personal requirements from CAP. I called the CAP and asked them about the criteria concerning Moderate or High complexity testing, after discussing with them the situations, IF you have a tech that is Licensed and Also has a QIHC, but does not minimum requirement Defined by CLIA in education ,, they CAN NOT do any QA/OC of IHC and antibody work up,, as IHC is defined as High complexity testing. I also asked about the test systems. The grandfather clause is only good for test systems that occurred for those time periods. For instance if CLIA defined the test system after those dates of 1997,, then they are not included and the person cannot perform test and technology created after those dates, since the testing was not in place during the grandfather clause time. In a nut shell meaning if the IHC staining and antibody was developed after those dates,, you are not covered by the grandfather clause to do the testing ,, can some help clear this up,, So any help on this matter will do ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PKRichar at gundersenhealth.org Wed Nov 23 08:43:24 2016 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Wed, 23 Nov 2016 14:43:24 +0000 Subject: [Histonet] Storage temps Message-ID: <998284C32F61104CA0BEFFFFCF6F90FD6D1C5197@LXEXMB01.gundluth.org> Does anyone know if there is a published acceptable range for storage of tissue and paraffin blocks? Cordially, Pam ~ +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org From BWhitney at pathgroup.com Wed Nov 23 10:41:06 2016 From: BWhitney at pathgroup.com (Brian Whitney) Date: Wed, 23 Nov 2016 16:41:06 +0000 Subject: [Histonet] Position Message-ID: We are a Histology lab located in the Blue Ridge Mountains of Virginia. We are currently looking for a staff Histology Technician. Certified preferred but will consider someone that is not. We average 260 blocks per day. If interested please contact my at my email below. Thanks, Brian Whitney HT (ASCP) Histology Manager PathGroup 1802 Braeburn Drive Salem, Va. 24153 cell 540-200-5995 phone: 540-777-1432 fax: 540-777-1449 bwhitney at pathgroup.com Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup immediately at 615-562-9255. Thank you From rjbuesa at yahoo.com Wed Nov 23 11:27:59 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 23 Nov 2016 17:27:59 +0000 (UTC) Subject: [Histonet] Storage temps In-Reply-To: <998284C32F61104CA0BEFFFFCF6F90FD6D1C5197@LXEXMB01.gundluth.org> References: <998284C32F61104CA0BEFFFFCF6F90FD6D1C5197@LXEXMB01.gundluth.org> Message-ID: <1063186432.555579.1479922079356@mail.yahoo.com> I do not know of anything published other than CAP "requirements" (unsubstantiated)Ren? On Wednesday, November 23, 2016 9:50 AM, "Richardson, Pam K via Histonet" wrote: Does anyone know if there is a published acceptable range for storage of tissue and paraffin blocks? Cordially, Pam ~ +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Wed Nov 23 11:31:19 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 23 Nov 2016 17:31:19 +0000 (UTC) Subject: [Histonet] Weigert's hematoxylin for IHC In-Reply-To: References: Message-ID: <727928075.567321.1479922279449@mail.yahoo.com> Once you finish the IHC procedure, the DAB reaction is very stable and you can use Weigert's or any other iron hematoxylin.Ren? On Tuesday, November 22, 2016 5:29 PM, Esther C Peters via Histonet wrote: Could someone advise me on whether Weigert's iron hematoxylin can be used as the counterstain for nuclei in IHC? We need to avoid using Harris's hematoxylin because that will stain mucocytes in the coral tissues we are working with. If it can be used, would that be after all antibody steps have been done? Thank you! Esther Peters Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epeters2 at gmu.edu http://esp.gmu.edu _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennifer.valentine-williams at neogenomics.com Wed Nov 23 16:02:16 2016 From: jennifer.valentine-williams at neogenomics.com (Jennifer Valentine-Williams) Date: Wed, 23 Nov 2016 22:02:16 +0000 Subject: [Histonet] Personel In-Reply-To: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu> Message-ID: <459bcf5a1a734f09ac1ed9b19046fcb6@tp1pvm-mbx2.neogen.local> I would like to branch off from this topic... Should a lab aid be allowed to load/unload slides/reagents from an automated IHC machine? Should they be permitted to print the labels that tell the machine which tests to run? I say no, but others say otherwise, so I'm interested in what everyone else here thinks. All lab tests have pre and post analytical components and I believe they all contribute to the High-complexity status of the "test". It surely cannot be that the interpretation is the only portion that is considered the "High-complexity test". I am open to hearing why this may be the case though. Jennifer Valentine-Williams, HT (ASCP) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secured or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. NeoGenomics Laboratories, Suite 5, 12701 Commonwealth Dr, Fort Myers, FL 33913, http://www.neogenomics.com (2016) From BWhitney at pathgroup.com Wed Nov 23 16:37:18 2016 From: BWhitney at pathgroup.com (Brian Whitney) Date: Wed, 23 Nov 2016 22:37:18 +0000 Subject: [Histonet] Personel In-Reply-To: <459bcf5a1a734f09ac1ed9b19046fcb6@tp1pvm-mbx2.neogen.local> References: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu>, <459bcf5a1a734f09ac1ed9b19046fcb6@tp1pvm-mbx2.neogen.local> Message-ID: The pathologist makes the determination as to if the controls are acceptable . We are looking into having a lab aide do the tasks you stated. Brian Sent from my iPhone > On Nov 23, 2016, at 5:21 PM, Jennifer Valentine-Williams via Histonet wrote: > > I would like to branch off from this topic... Should a lab aid be allowed to load/unload slides/reagents from an automated IHC machine? > Should they be permitted to print the labels that tell the machine which tests to run? > > I say no, but others say otherwise, so I'm interested in what everyone else here thinks. > > All lab tests have pre and post analytical components and I believe they all contribute to the High-complexity status of the "test". It surely cannot be that the interpretation is the only portion that is considered the "High-complexity test". I am open to hearing why this may be the case though. > > Jennifer Valentine-Williams, HT (ASCP) > > This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secured or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. NeoGenomics Laboratories, Suite 5, 12701 Commonwealth Dr, Fort Myers, FL 33913, http://www.neogenomics.com (2016) > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup immediately at 615-562-9255. Thank you From Timothy.Morken at ucsf.edu Wed Nov 23 17:55:07 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 23 Nov 2016 23:55:07 +0000 Subject: [Histonet] Personel In-Reply-To: <459bcf5a1a734f09ac1ed9b19046fcb6@tp1pvm-mbx2.neogen.local> References: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu> <459bcf5a1a734f09ac1ed9b19046fcb6@tp1pvm-mbx2.neogen.local> Message-ID: <761E2B5697F795489C8710BCC72141FF88E284D7@ex07.net.ucsf.edu> Jennifer, good question! That's why I listed the tasks and who may do them. From what I understand, in the clin lab simply loading samples on an analyser is considered moderate complexity - no prep of reagents. Just load pre-packaged reagents and the samples and push START. It seems that would apply to loading pre-dilute antibodies, pre-packaged detection kits, and loading slides. Maybe even programming the instrument if is not interfaced to an LIS. I think that CAP has invented the "test system" concept (I think they are the only ones using that term and concept) in order to convey that everyone one in the sample chain has a role in producing a valid result. I think that is a good idea to promote quality lab work. The "test system" expands the CLIA regulatory definition that a "test" is the interpretation and reporting of the final result and all the rest is "processing" a sample. Under CLIA the complexity concept does not apply at all to "processing personnel" (and so no competency testing is required). A "test system" implies that everyone in the chain has responsibility and must be competent Where CAP falls down is in not defining what parts of a "test system" are covered under the moderate- and high-complexity education requirements. They list their qualifications for various "testing personnel" in a chart, but the chart does not define which parts of any "test system" system are moderate or high complexity, or where complexity does not apply to personnel at all (is coverslipping high complexity if it is for a high complexity "test system?". How about sectioning?) Below is the algorithm used by FDA to score test as waived, medium or high complexity. Run a few common histology tasks thru this and see how many are high complexity. According the CLIA regs, all the complexity rolls up to the person interpreting and reporting results. They are responsible for the entire process. Score test according to criteria below Score 2 if any item is between 1 and 3 If it scores 12 and below it is moderate complexity Above 12 it is high complexity 1 - Knowledge ????????? Score 1. (A) Minimal scientific and technical knowledge is required to perform the test; and (B) Knowledge required to perform the test may be obtained through on-the-job instruction. ????????? Score 3. Specialized scientific and technical knowledge is essential to perform preanalytic, analytic or postanalytic phases of the testing. 2 - Training and experience ????????? Score 1. (A) Minimal training is required for preanalytic, analytic and postanalytic phases of the testing process; and (B) Limited experience is required to perform the test. ????????? Score 3. (A) Specialized training is essential to perform the preanalytic, analytic or postanalytic testing process; or Substantial experience may be necessary for analytic test performance. 3 - Reagents and materials preparation ????????? Score 1. (A) Reagents and materials are generally stable and reliable; and (B) Reagents and materials are prepackaged, or premeasured, or require no special handling, precautions or storage conditions. ????????? Score 3. (A) Reagents and materials may be labile and may require special handling to assure reliability; or (B) Reagents and materials preparation may include manual steps such as gravimetric or volumetric measurements. 4 - Characteristics of operational steps ????????? Score 1. Operational steps are either automatically executed (such as pipetting, temperature monitoring, or timing of steps), or are easily controlled. ????????? Score 3. Operational steps in the testing process require close monitoring or control, and may require special specimen preparation, precise temperature control or timing of procedural steps, accurate pipetting, or extensive calculations. 5 - Calibration, quality control, and proficiency testing materials ????????? Score 1. (A) Calibration materials are stable and readily available; (B) Quality control materials are stable and readily available; and (C) External proficiency testing materials, when available, are stable. ????????? Score 3. (A) Calibration materials, if available, may be labile; (B) Quality control materials may be labile, or not available; or (C) External proficiency testing materials, if available, may be labile. 6 - Test system troubleshooting and equipment maintenance ????????? Score 1. (A) Test system troubleshooting is automatic or self-correcting, or clearly described or requires minimal judgment; and (B) Equipment maintenance is provided by the manufacturer, is seldom needed, or can easily be performed. ????????? Score 3. (A) Troubleshooting is not automatic and requires decision-making and direct intervention to resolve most problems; or (B) Maintenance requires special knowledge, skills, and abilities. 7 - Interpretation and judgment ????????? Score 1. (A) Minimal interpretation and judgment are required to perform preanalytic, analytic and postanalytic processes; and (B) Resolution of problems requires limited independent interpretation and judgment. ????????? Score 3. (A) Extensive independent interpretation and judgment are required to perform the preanalytic, analytic or postanalytic processes; and (B) Resolution of problems requires extensive interpretation and judgment. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Jennifer Valentine-Williams [mailto:jennifer.valentine-williams at neogenomics.com] Sent: Wednesday, November 23, 2016 2:02 PM To: Morken, Timothy; Jesus Ellin Cc: Histonet Subject: RE: [Histonet] Personel I would like to branch off from this topic... Should a lab aid be allowed to load/unload slides/reagents from an automated IHC machine? Should they be permitted to print the labels that tell the machine which tests to run? I say no, but others say otherwise, so I'm interested in what everyone else here thinks. All lab tests have pre and post analytical components and I believe they all contribute to the High-complexity status of the "test". It surely cannot be that the interpretation is the only portion that is considered the "High-complexity test". I am open to hearing why this may be the case though. Jennifer Valentine-Williams, HT (ASCP) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secured or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. NeoGenomics Laboratories, Suite 5, 12701 Commonwealth Dr, Fort Myers, FL 33913, http://www.neogenomics.com (2016) From Timothy.Morken at ucsf.edu Wed Nov 23 18:12:06 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 24 Nov 2016 00:12:06 +0000 Subject: [Histonet] Personel In-Reply-To: <459bcf5a1a734f09ac1ed9b19046fcb6@tp1pvm-mbx2.neogen.local> References: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu> <459bcf5a1a734f09ac1ed9b19046fcb6@tp1pvm-mbx2.neogen.local> Message-ID: <761E2B5697F795489C8710BCC72141FF88E2850F@ex07.net.ucsf.edu> Jennifer, short answer: It is not that histology is not a "high complexity test." The issue is, who is defined as "testing personnel." All histology tests are high complexity. But in anatomic pathology the pathologist is the only designated "testing personnel" according to CLIA regs because they are the only personnel interpreting and reporting results. No one else in histology interprets or reports results, so all other work in histology is considered "processing." The confusion between AP and Clin Lab is that Med Techs do report out results and so are "testing personnel" under CLIA. CAP is trying to apply regs to AP that were written for Clin Lab. They not fit well.... Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Jennifer Valentine-Williams [mailto:jennifer.valentine-williams at neogenomics.com] Sent: Wednesday, November 23, 2016 2:02 PM To: Morken, Timothy; Jesus Ellin Cc: Histonet Subject: RE: [Histonet] Personel I would like to branch off from this topic... Should a lab aid be allowed to load/unload slides/reagents from an automated IHC machine? Should they be permitted to print the labels that tell the machine which tests to run? I say no, but others say otherwise, so I'm interested in what everyone else here thinks. All lab tests have pre and post analytical components and I believe they all contribute to the High-complexity status of the "test". It surely cannot be that the interpretation is the only portion that is considered the "High-complexity test". I am open to hearing why this may be the case though. Jennifer Valentine-Williams, HT (ASCP) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secured or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. NeoGenomics Laboratories, Suite 5, 12701 Commonwealth Dr, Fort Myers, FL 33913, http://www.neogenomics.com (2016) From Ronald.Houston at nationwidechildrens.org Fri Nov 25 08:02:15 2016 From: Ronald.Houston at nationwidechildrens.org (Houston, Ronald) Date: Fri, 25 Nov 2016 14:02:15 +0000 Subject: [Histonet] text-books for sale Message-ID: <5aadbe08e8a740f6bd4c9a7175daba05@l1perdwmbx02.childrensroot.net> Happy Thanksgiving My turn now; I have decided to retire mid-January and am starting to clear out my office. I have the following text-books for sale. There will be a standard charge of $5.50 for shipping and handling (irrespective of how many books you wish) A few books may have some highlighting, but the majority are in pristine condition. Please contact me off-line at ronniehh1349 at yahoo.com to order and for payment arrangements (will accept money order, check or PayPal) Management& Business Improvement The Idea Generator Tozawa B, Bodek N PCS Press 2001 $23.99 plus The Idea Generator Workbook - Quick and Easy Kaizen Tozawa B, Bodek N PCS Press 2002 Leadership Without Excuses Grimshaw J, Baron G McGraw Hill 2008 $11.99 Difficult Conversations Stone D, Patton B, Heen S Penguin 2010 $5.99 Coping With Difficult People in the Health Care Setting Umiker W ASCP Press 1994 (free with purchase of any one other management/business improvement book) Leadership and Self-Deception: Getting Out of the Box 2nd Edition The Arbinger Institute Berrett-Koehler Publishers 2015 $5.99 Why Leadership Sucks(TM): Fundamentals of Level 5 Leadership and Servant Leadership Smith, MA, Wolf M Kompelling Publishing 2012 $9.99 Managers Guide to Performance Reviews (free with purchase of any one other management/business improvement book) Beyond Heroes: A Lean Management System for Healthcare Barnas K ThedaCare Center for Healthcare Value 2012 $15.99 The Lean Turnaround: How Business Leaders Use Lean Principles to Create Value and Transform Their Company Byrne A, Womack J McGraw Hill 2012 $11.99 The Spirit of Kaizen Maurer R McGraw Hill 2012 $9.99 Gemba Kaizen: A Commonsense Approach to a Continuous Improvement Strategy, 2nd Edition Imai M McGraw Hill 2012 $11.99 The Pittsburgh Way to Efficient Healthcare Grunden N Healthcare Performance Press 2008 $16.25 Improving Healthcare Using Toyota Lean Production Methods, 2nd Edition Chalice R ASQ Press 2005 $8.50 Lean Thinking: Banish Waste and Create Wealth in your Corporation Womack JP, Jones DT Simon & Schuster 1996 (free with purchase of any one other management/business improvement book) What is Lean Six Sigma? George M, Rowlands D, Kastle B McGraw-Hill 2004 (free with purchase of any one other management/business improvement book) Lean Hospitals: Improving Quality, Patient Safety, and Employee Satisfaction Graban M CRC Press 2009 $6.75 Complete Idiot's Guide to Lean Six Sigma Breakthrough Management Group $5.25 Developing Lean Leaders at All Levels: A Practical Guide Liker JK Lean Leadership Institute Publications 2015 $21.75 Pathology & Medicine Scientific Imaging with Photoshop: Methods, Measurement, and Output Sedgewick J New Riders 2008 $100.25 Bone Marrow Immunohistochemistry Torlakovic EE, Naresh KN, Brunning RD ASCP 2008 $92.15 Diagnostic Histochemistry (with CD-ROM) Wick M Cambridge University Press 2008 $82.99 Tumors of Haematopoietic and Lymphoid Tissues Jaffe ES, Harris NL, Vardiman JW (eds) WHO - IARC $18.95 Pathology Informatics: Theory and Practice Pantanowitz, L ASCP 2012 $105.15 Practical Pathology Informatics: Demystifying informatics for the practicing anatomic pathologist Sinard, J Springer 2010 $30.55 Pathology of Chronic Constipation in Pediatric and Adult Coloproctology Meier-Rouge WA, Bruder E Karger 2005 $95.00 Schuster Atlas of Gastrointestinal Motility in Health and Disease(with CD-ROM) Schuster MM, Crowell MD, Koch KL BC Decker 2002 $15.99 The Second Brain: A Groundbreaking New Understanding of Nervous Disorders of the Stomach and Intestine Gershan MD Harper 1999 $17.25 Atlas of Pediatric Brain Tumors Adesina AM, Tihan T, Fuller CE, Poussaint TY Springer 2016 $55.75 Clinical Neuropathology: Text and Color Atlas Haberland C Demos 2006 $61.25 Modern Immunohistochemistry (with DVD-ROM) Chu P, Weiss L Cambridge 2014 $120.25 A Practical Guide to Frozen Section Technique Peters SR Springer 2009 $88.99 Color Atlas of Immunocytochemistry in Diagnostic Cytology Ganjel-Azar P, Nadji M Springer 2006 $65.95 Diagnostic Electron Microscopy: A Practical Guide to Interpretation and Technique Stirling J, Curry A, Eyden B Wiley 2013 $70.00 Handbook of Practical Immunohistochemistry: Frequently Asked Questions Lin F, Prichard J Springer 2015 $135.15 Immunomicroscopy: A Diagnostic Tool for the Surgical Pathologist 3rd Edition Taylor CR, Cote RJ Saunders 2005 $73.15 The Enteric Nervous System Furness JB Wiley-Blackwell 2006 $80.35 The Falling Sickness: a History of Epilepsy from the Greeks to the Beginnings of Modern Neurology Temkin, O John Hopkins University Press 1994 $16.35 A Disease Once Sacred: a History of the Medical Understanding of Epilepsy Eadie MJ, Bladin PF John Libbey 2001 $39.95 Pathology of Peripheral Nerves Weller RO, Cervos-Navarro J Butterworths 1977 $27.99 Color Atlas of Cytology, Histology, and Microscopic Anatomy Kuehnel W Thieme 2003 $15.89 Mayo Clinic Atlas of Immunofluorescence in Dermatology: Patterns and Target Antigens Kalaaji AN, Nicolas MEO Mayo Clinic Scientific Press 2006 $40.75 Interpretation of Immunofluorescent Patterns in Renal Diseases Valenzuela R, Deodhar SD ASCP 1994 $16.75 Microwave Techniques and Protocols Giberson RT, Demarjee RS (eds) Humana Press 2001 $101.99 All these books are also advertised on Amazon (at a higher price in most cases) and a few have already sold, just posted on Wednesday! There will be more coming over the next few days Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC FIBMS Anatomic Pathology Manager Laboratory Services 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston at nationwidechildrens.org www.NationwideChildrens.org "Without continual growth and progress, such words as improvement, achievement, and success have no meaning." ~ Ben Franklin From JEllin at yumaregional.org Mon Nov 28 08:03:32 2016 From: JEllin at yumaregional.org (Jesus Ellin) Date: Mon, 28 Nov 2016 14:03:32 +0000 Subject: [Histonet] Personel In-Reply-To: <761E2B5697F795489C8710BCC72141FF88E2850F@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu> <459bcf5a1a734f09ac1ed9b19046fcb6@tp1pvm-mbx2.neogen.local> <761E2B5697F795489C8710BCC72141FF88E2850F@ex07.net.ucsf.edu> Message-ID: I hope that everyone had a great weekend and a good thanksgiving,, Just to shed some light on the subject on the matter to have things in perspective, when I called CAP and here is what the Rep shared with me, it wasn't the interpretation or the result but rather the QA/QC of the result. See below: now here is the rub on this, as a person doing Special Stains, IHC, Digital Path and ISH,, we as TECH's QA/QC before we hand into the Pathologist, our controls are supposed to be reviewed by the tech to make sure the reaction took place, we don't say it looks brown, or red, or hey the colorful,, WE confirm the reaction, cause if not we redo. This is what ALSO the CAP states that we do because it does say Pathologist or Designee , and it's the QA/QC step. By no means are we resulting or giving Diagnosis/interpretation. This is where I am having trouble with this statement, about testing/interpretation,, it has nothing to do with that at all. CLIA also doesnt define QA/QC , just resulting/interpretation. They are the same Steps that are being followed on digital path, were imaging of the slides require a person of High Complexity testing to be doing the scanning. Again this is another QA/QC step, so I get all the stuff about CLIA and reporting, but CAP is specific and we are doing the QA/QC of this testing. I also had a conversation about whether Licensure was acceptable or not,, without meeting the requirements, and I was told NO,, they need to meet the minimum requirements as stated in CLIA for high complexity testing,, again something to look at. I then called ASCP to ask them about where they stood with this and was told we only do the licensure not the regs. I called CMS to get a better understanding stating the issue, as for them the person interpreting and reporting is the Pathologist, but if CAP requires that the QA/QC be done with those regs,, then that is more than is required, but that is their regulation and to be adhered too. Funny thing then when you go the CLIA website you get the high complexity testing, you can see where the testing methodologies are and can see when the test system came about. I know this is going to be a debatable situation and I have to agree with Tim,, please standardize and let us know,, but it is clear what they are saying about results, testing , interpretation and now QA/QC.. Thoughts anyone,, but I see that this is going to change histology as a whole, since now this is being added too the mix. I am not saying it is right or wrong,, but it does put forth effort that we need to classify what we do and also try to align the test system accordingly, because QA/QC is almost everything,, you can put this on instrumentation, protocols, procedures, etc. Also what do we do with those that have been doing this for years,, that have the knowledge and also the background,, again I don't wish to open up PANDORA's box,, I also know we are all going to look at this differently,, but the facts are we are changing and if we do not own the processes we currently do, someone else will that's for sure. Jesus Ellin **REVISED** 08/17/2016 ANP.21395 Special Stains/Studies Phase II For special stains, including histochemical stains, and studies using immunologic and ISH methodology, positive and negative controls are verified and recorded as acceptable prior to or concurrent with the reporting of patient results and records maintained. NOTE: Controls must be verified and recorded as acceptable by a pathologist or designee (provided the designee meets high complexity testing qualifications). Positive tissue controls must contain the component specific to the special stain that is being applied to the specimen. Immunohistochemical tests using polymer-based detection systems (biotin-free) are sufficiently free of background reactivity to obviate the need for a negative reagent control and such controls may be omitted at the discretion of the laboratory director following appropriate validation. If interpretation of the special stain or study is performed by a different laboratory, there must be a procedure for the laboratory performing the stain or study to verify the acceptability of the controls before transfer, if the controls are not sent with the patient slides (regardless of the outside laboratory's accrediting organization). Records of this verification must be readily ANP.23041 Testing Personnel Qualifications Phase II Personnel who are responsible for evaluating or accepting the imaging system data are qualified as high-complexity testing personnel. NOTE: The qualifications to perform high complexity testing can be accessed using the following link: CAP Personnel Requirements by Testing Complexity. available to the laboratory performing the interpretation. -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Wednesday, November 23, 2016 5:12 PM To: Jennifer Valentine-Williams; Jesus Ellin Cc: Histonet Subject: RE: [Histonet] Personel Jennifer, short answer: It is not that histology is not a "high complexity test." The issue is, who is defined as "testing personnel." All histology tests are high complexity. But in anatomic pathology the pathologist is the only designated "testing personnel" according to CLIA regs because they are the only personnel interpreting and reporting results. No one else in histology interprets or reports results, so all other work in histology is considered "processing." The confusion between AP and Clin Lab is that Med Techs do report out results and so are "testing personnel" under CLIA. CAP is trying to apply regs to AP that were written for Clin Lab. They not fit well.... Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Jennifer Valentine-Williams [mailto:jennifer.valentine-williams at neogenomics.com] Sent: Wednesday, November 23, 2016 2:02 PM To: Morken, Timothy; Jesus Ellin Cc: Histonet Subject: RE: [Histonet] Personel I would like to branch off from this topic... Should a lab aid be allowed to load/unload slides/reagents from an automated IHC machine? Should they be permitted to print the labels that tell the machine which tests to run? I say no, but others say otherwise, so I'm interested in what everyone else here thinks. All lab tests have pre and post analytical components and I believe they all contribute to the High-complexity status of the "test". It surely cannot be that the interpretation is the only portion that is considered the "High-complexity test". I am open to hearing why this may be the case though. Jennifer Valentine-Williams, HT (ASCP) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secured or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. NeoGenomics Laboratories, Suite 5, 12701 Commonwealth Dr, Fort Myers, FL 33913, http://www.neogenomics.com (2016) ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From Donna.Willis at BSWHealth.org Mon Nov 28 09:25:18 2016 From: Donna.Willis at BSWHealth.org (Willis, Donna G.) Date: Mon, 28 Nov 2016 15:25:18 +0000 Subject: [Histonet] Personel In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu> <459bcf5a1a734f09ac1ed9b19046fcb6@tp1pvm-mbx2.neogen.local> <761E2B5697F795489C8710BCC72141FF88E2850F@ex07.net.ucsf.edu> Message-ID: My interpretation of this is that for AP the "test" is the reporting of the results. What is done in the Histology lab to prepare the slides is the pre-analytical portion of the test. For the revised ANP. 21395 the pathologist is mentioning in the report the results of the controls therefore it is in concurrent with the report. We still check our results before taking the slides to the pathologist. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas? 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com -----Original Message----- From: Jesus Ellin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, November 28, 2016 8:04 AM To: Morken, Timothy; Jennifer Valentine-Williams Cc: Histonet Subject: [EXTERNAL] Re: [Histonet] Personel I hope that everyone had a great weekend and a good thanksgiving,, Just to shed some light on the subject on the matter to have things in perspective, when I called CAP and here is what the Rep shared with me, it wasn't the interpretation or the result but rather the QA/QC of the result. See below: now here is the rub on this, as a person doing Special Stains, IHC, Digital Path and ISH,, we as TECH's QA/QC before we hand into the Pathologist, our controls are supposed to be reviewed by the tech to make sure the reaction took place, we don't say it looks brown, or red, or hey the colorful,, WE confirm the reaction, cause if not we redo. This is what ALSO the CAP states that we do because it does say Pathologist or Designee , and it's the QA/QC step. By no means are we resulting or giving Diagnosis/interpretation. This is where I am having trouble with this statement, about testing/interpretation,, it has nothing to do with that at all. CLIA also doesnt define QA/QC , just resulting/interpretation. They are the same Steps that are being followed on digital path, were imaging of the slides require a person of High Complexity testing to be doing the scanning. Again this is another QA/QC step, so I get all the stuff about CLIA and reporting, but CAP is specific and we are doing the QA/QC of this testing. I also had a conversation about whether Licensure was acceptable or not,, without meeting the requirements, and I was told NO,, they need to meet the minimum requirements as stated in CLIA for high complexity testing,, again something to look at. I then called ASCP to ask them about where they stood with this and was told we only do the licensure not the regs. I called CMS to get a better understanding stating the issue, as for them the person interpreting and reporting is the Pathologist, but if CAP requires that the QA/QC be done with those regs,, then that is more than is required, but that is their regulation and to be adhered too. Funny thing then when you go the CLIA website you get the high complexity testing, you can see where the testing methodologies are and can see when the test system came about. I know this is going to be a debatable situation and I have to agree with Tim,, please standardize and let us know,, but it is clear what they are saying about results, testing , interpretation and now QA/QC.. Thoughts anyone,, but I see that this is going to change histology as a whole, since now this is being added too the mix. I am not saying it is right or wrong,, but it does put forth effort that we need to classify what we do and also try to align the test system accordingly, because QA/QC is almost everything,, you can put this on instrumentation, protocols, procedures, etc. Also what do we do with those that have been doing this for years,, that have the knowledge and also the background,, again I don't wish to open up PANDORA's box,, I also know we are all going to look at this differently,, but the facts are we are changing and if we do not own the processes we currently do, someone else will that's for sure. Jesus Ellin **REVISED** 08/17/2016 ANP.21395 Special Stains/Studies Phase II For special stains, including histochemical stains, and studies using immunologic and ISH methodology, positive and negative controls are verified and recorded as acceptable prior to or concurrent with the reporting of patient results and records maintained. NOTE: Controls must be verified and recorded as acceptable by a pathologist or designee (provided the designee meets high complexity testing qualifications). Positive tissue controls must contain the component specific to the special stain that is being applied to the specimen. Immunohistochemical tests using polymer-based detection systems (biotin-free) are sufficiently free of background reactivity to obviate the need for a negative reagent control and such controls may be omitted at the discretion of the laboratory director following appropriate validation. If interpretation of the special stain or study is performed by a different laboratory, there must be a procedure for the laboratory performing the stain or study to verify the acceptability of the controls before transfer, if the controls are not sent with the patient slides (regardless of the outside laboratory's accrediting organization). Records of this verification must be readily ANP.23041 Testing Personnel Qualifications Phase II Personnel who are responsible for evaluating or accepting the imaging system data are qualified as high-complexity testing personnel. NOTE: The qualifications to perform high complexity testing can be accessed using the following link: CAP Personnel Requirements by Testing Complexity. available to the laboratory performing the interpretation. -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Wednesday, November 23, 2016 5:12 PM To: Jennifer Valentine-Williams; Jesus Ellin Cc: Histonet Subject: RE: [Histonet] Personel Jennifer, short answer: It is not that histology is not a "high complexity test." The issue is, who is defined as "testing personnel." All histology tests are high complexity. But in anatomic pathology the pathologist is the only designated "testing personnel" according to CLIA regs because they are the only personnel interpreting and reporting results. No one else in histology interprets or reports results, so all other work in histology is considered "processing." The confusion between AP and Clin Lab is that Med Techs do report out results and so are "testing personnel" under CLIA. CAP is trying to apply regs to AP that were written for Clin Lab. They not fit well.... Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Jennifer Valentine-Williams [mailto:jennifer.valentine-williams at neogenomics.com] Sent: Wednesday, November 23, 2016 2:02 PM To: Morken, Timothy; Jesus Ellin Cc: Histonet Subject: RE: [Histonet] Personel I would like to branch off from this topic... Should a lab aid be allowed to load/unload slides/reagents from an automated IHC machine? Should they be permitted to print the labels that tell the machine which tests to run? I say no, but others say otherwise, so I'm interested in what everyone else here thinks. All lab tests have pre and post analytical components and I believe they all contribute to the High-complexity status of the "test". It surely cannot be that the interpretation is the only portion that is considered the "High-complexity test". I am open to hearing why this may be the case though. Jennifer Valentine-Williams, HT (ASCP) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secured or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. NeoGenomics Laboratories, Suite 5, 12701 Commonwealth Dr, Fort Myers, FL 33913, https://urldefense.proofpoint.com/v2/url?u=http-3A__www.neogenomics.com&d=DgICAg&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=sIsP8AdMBDzEQGVQ2pzyoVbwlzaIaR_I0w3z1do5568&m=RdBcK3tybqPbnBWOzTtsW-N8Xlp2DQZ8lPawSoQutFk&s=hxHk3_6nFf5TDhfVGkKqtiXSvUK84BfDKbke6XzRcdU&e= (2016) ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DgICAg&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=sIsP8AdMBDzEQGVQ2pzyoVbwlzaIaR_I0w3z1do5568&m=RdBcK3tybqPbnBWOzTtsW-N8Xlp2DQZ8lPawSoQutFk&s=_AstA1CyCXhYTqN6Y9STGXsdes6GSbUp8O-4xpjzlpg&e= ********************************************************************** The information contained in this e-mail may be privileged and/or confidential, and protected from disclosure, and no waiver of any attorney-client, work product, or other privilege is intended. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. The sender and Baylor Scott & White Health, and its affiliated entities, hereby expressly reserve all privileges and confidentiality that might otherwise be waived as a result of an erroneous or misdirected e-mail transmission. No employee or agent is authorized to conclude any binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, by e-mail without express written confirmation by the CEO, the Senior Vice President of Supply Chain Services or other duly authorized representative of Baylor Scott & White Health. From JEllin at yumaregional.org Mon Nov 28 09:47:16 2016 From: JEllin at yumaregional.org (Jesus Ellin) Date: Mon, 28 Nov 2016 15:47:16 +0000 Subject: [Histonet] Personel In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu> <459bcf5a1a734f09ac1ed9b19046fcb6@tp1pvm-mbx2.neogen.local> <761E2B5697F795489C8710BCC72141FF88E2850F@ex07.net.ucsf.edu> Message-ID: Like I stated there is a lot out there and I agree with you all interpretation of this can vary,, but again our world is changing and I hope our powers at be are being the advocate for these issues,, cause we then are forces to make the interpretations, which I get it we are again asking the Pathologist to do,, but this section in under General Quality Control,, not at all added to the report,, the report itself has this designated as well in that section as well as the section for predictive markers,, So again you can see the confusion and ambiguity of this situation. We need those advocates to make this clear for us for sure -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis at BSWHealth.org] Sent: Monday, November 28, 2016 8:25 AM To: Jesus Ellin; Morken, Timothy; Jennifer Valentine-Williams Cc: histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] Personel My interpretation of this is that for AP the "test" is the reporting of the results. What is done in the Histology lab to prepare the slides is the pre-analytical portion of the test. For the revised ANP. 21395 the pathologist is mentioning in the report the results of the controls therefore it is in concurrent with the report. We still check our results before taking the slides to the pathologist. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas? 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com -----Original Message----- From: Jesus Ellin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, November 28, 2016 8:04 AM To: Morken, Timothy; Jennifer Valentine-Williams Cc: Histonet Subject: [EXTERNAL] Re: [Histonet] Personel I hope that everyone had a great weekend and a good thanksgiving,, Just to shed some light on the subject on the matter to have things in perspective, when I called CAP and here is what the Rep shared with me, it wasn't the interpretation or the result but rather the QA/QC of the result. See below: now here is the rub on this, as a person doing Special Stains, IHC, Digital Path and ISH,, we as TECH's QA/QC before we hand into the Pathologist, our controls are supposed to be reviewed by the tech to make sure the reaction took place, we don't say it looks brown, or red, or hey the colorful,, WE confirm the reaction, cause if not we redo. This is what ALSO the CAP states that we do because it does say Pathologist or Designee , and it's the QA/QC step. By no means are we resulting or giving Diagnosis/interpretation. This is where I am having trouble with this statement, about testing/interpretation,, it has nothing to do with that at all. CLIA also doesnt define QA/QC , just resulting/interpretation. They are the same Steps that are being followed on digital path, were imaging of the slides require a person of High Complexity testing to be doing the scanning. Again this is another QA/QC step, so I get all the stuff about CLIA and reporting, but CAP is specific and we are doing the QA/QC of this testing. I also had a conversation about whether Licensure was acceptable or not,, without meeting the requirements, and I was told NO,, they need to meet the minimum requirements as stated in CLIA for high complexity testing,, again something to look at. I then called ASCP to ask them about where they stood with this and was told we only do the licensure not the regs. I called CMS to get a better understanding stating the issue, as for them the person interpreting and reporting is the Pathologist, but if CAP requires that the QA/QC be done with those regs,, then that is more than is required, but that is their regulation and to be adhered too. Funny thing then when you go the CLIA website you get the high complexity testing, you can see where the testing methodologies are and can see when the test system came about. I know this is going to be a debatable situation and I have to agree with Tim,, please standardize and let us know,, but it is clear what they are saying about results, testing , interpretation and now QA/QC.. Thoughts anyone,, but I see that this is going to change histology as a whole, since now this is being added too the mix. I am not saying it is right or wrong,, but it does put forth effort that we need to classify what we do and also try to align the test system accordingly, because QA/QC is almost everything,, you can put this on instrumentation, protocols, procedures, etc. Also what do we do with those that have been doing this for years,, that have the knowledge and also the background,, again I don't wish to open up PANDORA's box,, I also know we are all going to look at this differently,, but the facts are we are changing and if we do not own the processes we currently do, someone else will that's for sure. Jesus Ellin **REVISED** 08/17/2016 ANP.21395 Special Stains/Studies Phase II For special stains, including histochemical stains, and studies using immunologic and ISH methodology, positive and negative controls are verified and recorded as acceptable prior to or concurrent with the reporting of patient results and records maintained. NOTE: Controls must be verified and recorded as acceptable by a pathologist or designee (provided the designee meets high complexity testing qualifications). Positive tissue controls must contain the component specific to the special stain that is being applied to the specimen. Immunohistochemical tests using polymer-based detection systems (biotin-free) are sufficiently free of background reactivity to obviate the need for a negative reagent control and such controls may be omitted at the discretion of the laboratory director following appropriate validation. If interpretation of the special stain or study is performed by a different laboratory, there must be a procedure for the laboratory performing the stain or study to verify the acceptability of the controls before transfer, if the controls are not sent with the patient slides (regardless of the outside laboratory's accrediting organization). Records of this verification must be readily ANP.23041 Testing Personnel Qualifications Phase II Personnel who are responsible for evaluating or accepting the imaging system data are qualified as high-complexity testing personnel. NOTE: The qualifications to perform high complexity testing can be accessed using the following link: CAP Personnel Requirements by Testing Complexity. available to the laboratory performing the interpretation. -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Wednesday, November 23, 2016 5:12 PM To: Jennifer Valentine-Williams; Jesus Ellin Cc: Histonet Subject: RE: [Histonet] Personel Jennifer, short answer: It is not that histology is not a "high complexity test." The issue is, who is defined as "testing personnel." All histology tests are high complexity. But in anatomic pathology the pathologist is the only designated "testing personnel" according to CLIA regs because they are the only personnel interpreting and reporting results. No one else in histology interprets or reports results, so all other work in histology is considered "processing." The confusion between AP and Clin Lab is that Med Techs do report out results and so are "testing personnel" under CLIA. CAP is trying to apply regs to AP that were written for Clin Lab. They not fit well.... Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Jennifer Valentine-Williams [mailto:jennifer.valentine-williams at neogenomics.com] Sent: Wednesday, November 23, 2016 2:02 PM To: Morken, Timothy; Jesus Ellin Cc: Histonet Subject: RE: [Histonet] Personel I would like to branch off from this topic... Should a lab aid be allowed to load/unload slides/reagents from an automated IHC machine? Should they be permitted to print the labels that tell the machine which tests to run? I say no, but others say otherwise, so I'm interested in what everyone else here thinks. All lab tests have pre and post analytical components and I believe they all contribute to the High-complexity status of the "test". It surely cannot be that the interpretation is the only portion that is considered the "High-complexity test". I am open to hearing why this may be the case though. Jennifer Valentine-Williams, HT (ASCP) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secured or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. NeoGenomics Laboratories, Suite 5, 12701 Commonwealth Dr, Fort Myers, FL 33913, https://urldefense.proofpoint.com/v2/url?u=http-3A__www.neogenomics.com&d=DgICAg&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=sIsP8AdMBDzEQGVQ2pzyoVbwlzaIaR_I0w3z1do5568&m=RdBcK3tybqPbnBWOzTtsW-N8Xlp2DQZ8lPawSoQutFk&s=hxHk3_6nFf5TDhfVGkKqtiXSvUK84BfDKbke6XzRcdU&e= (2016) ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DgICAg&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=sIsP8AdMBDzEQGVQ2pzyoVbwlzaIaR_I0w3z1do5568&m=RdBcK3tybqPbnBWOzTtsW-N8Xlp2DQZ8lPawSoQutFk&s=_AstA1CyCXhYTqN6Y9STGXsdes6GSbUp8O-4xpjzlpg&e= ********************************************************************** The information contained in this e-mail may be privileged and/or confidential, and protected from disclosure, and no waiver of any attorney-client, work product, or other privilege is intended. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. The sender and Baylor Scott & White Health, and its affiliated entities, hereby expressly reserve all privileges and confidentiality that might otherwise be waived as a result of an erroneous or misdirected e-mail transmission. No employee or agent is authorized to conclude any binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, by e-mail without express written confirmation by the CEO, the Senior Vice President of Supply Chain Services or other duly authorized representative of Baylor Scott & White Health. ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ From tkngflght at yahoo.com Mon Nov 28 10:54:24 2016 From: tkngflght at yahoo.com (Cheryl) Date: Mon, 28 Nov 2016 16:54:24 +0000 (UTC) Subject: [Histonet] Job description for a Pathologist (employed, not contracted) References: <1215381251.1793621.1480352064209.ref@mail.yahoo.com> Message-ID: <1215381251.1793621.1480352064209@mail.yahoo.com> Hi Guys- Looking for a job description for a Pathologist. ?Most interested in the odd bits vs. core responsibilities and how much detail goes into the list... Anything you can share would be helpful!! Cheryl?Cheryl Kerry, HT(ASCP) Full Staff Inc. ? admin at fullstaff.org?800.756.3309 Phone & Fax https://www.facebook.com/TheHistologyCompany/ From Timothy.Morken at ucsf.edu Mon Nov 28 11:00:43 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 28 Nov 2016 17:00:43 +0000 Subject: [Histonet] Personel In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF88E27F9C@ex07.net.ucsf.edu> <459bcf5a1a734f09ac1ed9b19046fcb6@tp1pvm-mbx2.neogen.local> <761E2B5697F795489C8710BCC72141FF88E2850F@ex07.net.ucsf.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF88E28B67@ex07.net.ucsf.edu> Just to throw another wrench in the works, we just had a "friendly" Joint Commission inspection (we are under JC) to preview coming regs. The inspector this time, for the first time, said ALL QC must be done by a "qualified pathologist." Even H&E slide we do before starting stain runs. We have always had techs QC the H&E and special stains. IHC has always been done by a pathologist. Now they want all done by a pathologist. The inspector wouldn't even consider that a tech could do it, whether qualified for high complexity or not. But the actual "QSA" element they quoted is somewhat ambiguous in that it specifies a qualified pathologist must "...assess the staining quality (for example equipment, methods, stains) of microscopic tissue sections to determine the stains ability to facilitate a diagnosis." Then it goes on to say the "Laboratory" performs controls for each type of stain. We always took this to mean a pathologist approves the validation of stains, but the techs in the lab can do the QC when designated to do so. Now it is a big question mark. Again, the Deemed Agency for accreditation (CAP or JC) may institute stricter standards than the original CLIA regs so can say if your techs QC slides, then they must qualify for high complexity testing. CLIA does not consider QC of histology slides to be a "test" so does not require specific qualifications to run QC slides. Note that a Med Tech already has High Complexity qualifications, is a "testing personnel," reports actual test results and will also do QC as part of their job. So their "QC" situation should not be compared to histology. The CAP general checklist item that covers the Clin Lab is not necessarily applicable to the histo lab. Again, CAP has not stated anywhere an explicit definition of what the high complexity component of IHC staining is. However, it seems labs can get around the whole CAP information gap by just having pathologists QC all controls. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Jesus Ellin [mailto:JEllin at yumaregional.org] Sent: Monday, November 28, 2016 7:47 AM To: Willis, Donna G.; Morken, Timothy; Jennifer Valentine-Williams Cc: histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] Personel Like I stated there is a lot out there and I agree with you all interpretation of this can vary,, but again our world is changing and I hope our powers at be are being the advocate for these issues,, cause we then are forces to make the interpretations, which I get it we are again asking the Pathologist to do,, but this section in under General Quality Control,, not at all added to the report,, the report itself has this designated as well in that section as well as the section for predictive markers,, So again you can see the confusion and ambiguity of this situation. We need those advocates to make this clear for us for sure -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis at BSWHealth.org] Sent: Monday, November 28, 2016 8:25 AM To: Jesus Ellin; Morken, Timothy; Jennifer Valentine-Williams Cc: histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] Personel My interpretation of this is that for AP the "test" is the reporting of the results. What is done in the Histology lab to prepare the slides is the pre-analytical portion of the test. For the revised ANP. 21395 the pathologist is mentioning in the report the results of the controls therefore it is in concurrent with the report. We still check our results before taking the slides to the pathologist. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas? 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com -----Original Message----- From: Jesus Ellin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, November 28, 2016 8:04 AM To: Morken, Timothy; Jennifer Valentine-Williams Cc: Histonet Subject: [EXTERNAL] Re: [Histonet] Personel I hope that everyone had a great weekend and a good thanksgiving,, Just to shed some light on the subject on the matter to have things in perspective, when I called CAP and here is what the Rep shared with me, it wasn't the interpretation or the result but rather the QA/QC of the result. See below: now here is the rub on this, as a person doing Special Stains, IHC, Digital Path and ISH,, we as TECH's QA/QC before we hand into the Pathologist, our controls are supposed to be reviewed by the tech to make sure the reaction took place, we don't say it looks brown, or red, or hey the colorful,, WE confirm the reaction, cause if not we redo. This is what ALSO the CAP states that we do because it does say Pathologist or Designee , and it's the QA/QC step. By no means are we resulting or giving Diagnosis/interpretation. This is where I am having trouble with this statement, about testing/interpretation,, it has nothing to do with that at all. CLIA also doesnt define QA/QC , just resulting/interpretation. They are the same Steps that are being followed on digital path, were imaging of the slides require a person of High Complexity testing to be doing the scanning. Again this is another QA/QC step, so I get all the stuff about CLIA and reporting, but CAP is specific and we are doing the QA/QC of this testing. I also had a conversation about whether Licensure was acceptable or not,, without meeting the requirements, and I was told NO,, they need to meet the minimum requirements as stated in CLIA for high complexity testing,, again something to look at. I then called ASCP to ask them about where they stood with this and was told we only do the licensure not the regs. I called CMS to get a better understanding stating the issue, as for them the person interpreting and reporting is the Pathologist, but if CAP requires that the QA/QC be done with those regs,, then that is more than is required, but that is their regulation and to be adhered too. Funny thing then when you go the CLIA website you get the high complexity testing, you can see where the testing methodologies are and can see when the test system came about. I know this is going to be a debatable situation and I have to agree with Tim,, please standardize and let us know,, but it is clear what they are saying about results, testing , interpretation and now QA/QC.. Thoughts anyone,, but I see that this is going to change histology as a whole, since now this is being added too the mix. I am not saying it is right or wrong,, but it does put forth effort that we need to classify what we do and also try to align the test system accordingly, because QA/QC is almost everything,, you can put this on instrumentation, protocols, procedures, etc. Also what do we do with those that have been doing this for years,, that have the knowledge and also the background,, again I don't wish to open up PANDORA's box,, I also know we are all going to look at this differently,, but the facts are we are changing and if we do not own the processes we currently do, someone else will that's for sure. Jesus Ellin **REVISED** 08/17/2016 ANP.21395 Special Stains/Studies Phase II For special stains, including histochemical stains, and studies using immunologic and ISH methodology, positive and negative controls are verified and recorded as acceptable prior to or concurrent with the reporting of patient results and records maintained. NOTE: Controls must be verified and recorded as acceptable by a pathologist or designee (provided the designee meets high complexity testing qualifications). Positive tissue controls must contain the component specific to the special stain that is being applied to the specimen. Immunohistochemical tests using polymer-based detection systems (biotin-free) are sufficiently free of background reactivity to obviate the need for a negative reagent control and such controls may be omitted at the discretion of the laboratory director following appropriate validation. If interpretation of the special stain or study is performed by a different laboratory, there must be a procedure for the laboratory performing the stain or study to verify the acceptability of the controls before transfer, if the controls are not sent with the patient slides (regardless of the outside laboratory's accrediting organization). Records of this verification must be readily ANP.23041 Testing Personnel Qualifications Phase II Personnel who are responsible for evaluating or accepting the imaging system data are qualified as high-complexity testing personnel. NOTE: The qualifications to perform high complexity testing can be accessed using the following link: CAP Personnel Requirements by Testing Complexity. available to the laboratory performing the interpretation. -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Wednesday, November 23, 2016 5:12 PM To: Jennifer Valentine-Williams; Jesus Ellin Cc: Histonet Subject: RE: [Histonet] Personel Jennifer, short answer: It is not that histology is not a "high complexity test." The issue is, who is defined as "testing personnel." All histology tests are high complexity. But in anatomic pathology the pathologist is the only designated "testing personnel" according to CLIA regs because they are the only personnel interpreting and reporting results. No one else in histology interprets or reports results, so all other work in histology is considered "processing." The confusion between AP and Clin Lab is that Med Techs do report out results and so are "testing personnel" under CLIA. CAP is trying to apply regs to AP that were written for Clin Lab. They not fit well.... Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Jennifer Valentine-Williams [mailto:jennifer.valentine-williams at neogenomics.com] Sent: Wednesday, November 23, 2016 2:02 PM To: Morken, Timothy; Jesus Ellin Cc: Histonet Subject: RE: [Histonet] Personel I would like to branch off from this topic... Should a lab aid be allowed to load/unload slides/reagents from an automated IHC machine? Should they be permitted to print the labels that tell the machine which tests to run? I say no, but others say otherwise, so I'm interested in what everyone else here thinks. All lab tests have pre and post analytical components and I believe they all contribute to the High-complexity status of the "test". It surely cannot be that the interpretation is the only portion that is considered the "High-complexity test". I am open to hearing why this may be the case though. Jennifer Valentine-Williams, HT (ASCP) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secured or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. NeoGenomics Laboratories, Suite 5, 12701 Commonwealth Dr, Fort Myers, FL 33913, https://urldefense.proofpoint.com/v2/url?u=http-3A__www.neogenomics.com&d=DgICAg&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=sIsP8AdMBDzEQGVQ2pzyoVbwlzaIaR_I0w3z1do5568&m=RdBcK3tybqPbnBWOzTtsW-N8Xlp2DQZ8lPawSoQutFk&s=hxHk3_6nFf5TDhfVGkKqtiXSvUK84BfDKbke6XzRcdU&e= (2016) ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DgICAg&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=sIsP8AdMBDzEQGVQ2pzyoVbwlzaIaR_I0w3z1do5568&m=RdBcK3tybqPbnBWOzTtsW-N8Xlp2DQZ8lPawSoQutFk&s=_AstA1CyCXhYTqN6Y9STGXsdes6GSbUp8O-4xpjzlpg&e= ********************************************************************** The information contained in this e-mail may be privileged and/or confidential, and protected from disclosure, and no waiver of any attorney-client, work product, or other privilege is intended. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. The sender and Baylor Scott & White Health, and its affiliated entities, hereby expressly reserve all privileges and confidentiality that might otherwise be waived as a result of an erroneous or misdirected e-mail transmission. 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Thank You. ______________________________________________________________________ From Jaime.Reuss at cchmc.org Mon Nov 28 13:27:20 2016 From: Jaime.Reuss at cchmc.org (Reuss, Jaime) Date: Mon, 28 Nov 2016 19:27:20 +0000 Subject: [Histonet] Sectioning TMA's Message-ID: <6046EC36-5807-4A52-8968-17D38F50B515@cchmc.org> Hello all! I am using a semi-automated tissue micro arrayer to create blocks. I have started sectioning but a lot of the cores are rolling up. The specific tissue is brain tumor. Any hints on how to prevent this? Thanks! From mgiorgi at incdx.com Mon Nov 28 17:15:39 2016 From: mgiorgi at incdx.com (Miranda Giorgi) Date: Mon, 28 Nov 2016 23:15:39 +0000 Subject: [Histonet] Histology Supvisor Job Openning (Bellevue, WA) Message-ID: <932d8ecf6bb04c79b18a9ad113b70e74@SPOWSAPP05.icp.priv> Incyte Diagnostics is looking for a qualified individual to help oversee the day to day activities of the Histology department at our Bellevue, WA facility. Along with ensuring accurate and timely processing of specimens and compliance with CLIA and CAP guidelines, this individual will help manage department quality and productivity while monitoring costs. The histology supervisor works with the histology manager to help drive continuous improvement and standardization in all aspects of the operations. Incyte Diagnostics offers great jobs, great pay, great benefits and a great place to work. If you or anyone you know is interested, please check out the link below: http://incytediagnostics.applicantstack.com/x/openings This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700. From sandra.cheasty at wisc.edu Tue Nov 29 14:01:04 2016 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Tue, 29 Nov 2016 20:01:04 +0000 Subject: [Histonet] Long Pipette Sharps Disposal Container Message-ID: Hello all, Can anyone recommend an economical sharps disposal container for long plastic pipettes? Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From jclark at pcnm.com Tue Nov 29 15:09:17 2016 From: jclark at pcnm.com (Joanne Clark) Date: Tue, 29 Nov 2016 21:09:17 +0000 Subject: [Histonet] ER/PR Uneven Staining Message-ID: <57ae34a9ab5a465384e9fa4a582db780@E15MADAG-D06N04.sh11.lan> I had a breast needle core today that when stained with ER and PR the staining was uneven throughout the core, even though the cancer cells were present in the entire core. The specimen had 10 hours fixation in 10% NBF. I could understand the uneven staining from inadequate fixation on large grossed in breast tissue, but 10 hours with needle core biopsies has always been more than sufficient. Does anyone have any ideas? We use Leica's ER and PR antibodies on the BOND. Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Richard.Cartun at hhchealth.org Tue Nov 29 16:11:37 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 29 Nov 2016 22:11:37 +0000 Subject: [Histonet] ER/PR Uneven Staining In-Reply-To: <57ae34a9ab5a465384e9fa4a582db780@E15MADAG-D06N04.sh11.lan> References: <57ae34a9ab5a465384e9fa4a582db780@E15MADAG-D06N04.sh11.lan> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E953CF0F3@HHCEXCHMB03.hhcsystem.org> Can you provide more details about the uneven immunoreactivity? Are the negative cells located at the periphery of the specimen or are the negative and positive tumor cells mixed together throughout the specimen? Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Joanne Clark via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 29, 2016 4:09 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] ER/PR Uneven Staining I had a breast needle core today that when stained with ER and PR the staining was uneven throughout the core, even though the cancer cells were present in the entire core. The specimen had 10 hours fixation in 10% NBF. I could understand the uneven staining from inadequate fixation on large grossed in breast tissue, but 10 hours with needle core biopsies has always been more than sufficient. Does anyone have any ideas? We use Leica's ER and PR antibodies on the BOND. Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From jclark at pcnm.com Tue Nov 29 16:24:31 2016 From: jclark at pcnm.com (Joanne Clark) Date: Tue, 29 Nov 2016 22:24:31 +0000 Subject: [Histonet] ER/PR Uneven Staining In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E953CF0F3@HHCEXCHMB03.hhcsystem.org> References: <57ae34a9ab5a465384e9fa4a582db780@E15MADAG-D06N04.sh11.lan> <9215BD4B0BA1B44D962A71C758B68D2E953CF0F3@HHCEXCHMB03.hhcsystem.org> Message-ID: It's rather strange. There are positive tumor cells throughout the needle core, but the bottom third is staining very strongly all across the core, then there is a small portion where the staining is there but not as strongly as the section below it, then another segment above this where the staining is strong again. The strong and weak staining is across the entire core of the tissue, not just at the edges. -----Original Message----- From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] Sent: Tuesday, November 29, 2016 3:12 PM To: Joanne Clark Cc: histonet at lists.utsouthwestern.edu Subject: RE: ER/PR Uneven Staining Can you provide more details about the uneven immunoreactivity? Are the negative cells located at the periphery of the specimen or are the negative and positive tumor cells mixed together throughout the specimen? Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Joanne Clark via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 29, 2016 4:09 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] ER/PR Uneven Staining I had a breast needle core today that when stained with ER and PR the staining was uneven throughout the core, even though the cancer cells were present in the entire core. The specimen had 10 hours fixation in 10% NBF. I could understand the uneven staining from inadequate fixation on large grossed in breast tissue, but 10 hours with needle core biopsies has always been more than sufficient. Does anyone have any ideas? We use Leica's ER and PR antibodies on the BOND. Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From LRaff at uropartners.com Wed Nov 30 07:36:29 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 30 Nov 2016 13:36:29 +0000 Subject: [Histonet] Happy Holidays and Pathology Related Post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF110F9603@COLOEXCH01.uropartners.local> Hope everyone has recovered from Thanksgiving and is looking forward to the holidays ahead, whatever you may celebrate. The new blog post is seeking data on pathology reports. Feel free to read and share. http://www.chicagonow.com/downsize-maybe/2016/11/if-you-have-had-surgery-or-a-biopsy-you-have-had-a-pathology-report-did-you-read-and-understand-it/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From tbraud at holyredeemer.com Wed Nov 30 12:38:02 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 30 Nov 2016 18:38:02 +0000 Subject: [Histonet] Uneven ER/PR Message-ID: <48E053DDF6CE074DB6A7414BA05403F8112C87@HRHEX03-HOS.holyredeemer.local> My first 2 items to check whenever I have uneven IHC staining are (1) Inadequate deparaffinization, (2) bad lot of charged slides - yes this can cause terribly streaky or spotty staining, and since you are using the Bond, perhaps there was an issue with coverplate placements. Just things to consider. Hope this helps, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Today's Topics: 2. ER/PR Uneven Staining (Joanne Clark) Message: 2 Date: Tue, 29 Nov 2016 21:09:17 +0000 From: Joanne Clark Subject: [Histonet] ER/PR Uneven Staining I had a breast needle core today that when stained with ER and PR the staining was uneven throughout the core, even though the cancer cells were present in the entire core. The specimen had 10 hours fixation in 10% NBF. I could understand the uneven staining from inadequate fixation on large grossed in breast tissue, but 10 hours with needle core biopsies has always been more than sufficient. Does anyone have any ideas? We use Leica's ER and PR antibodies on the BOND. Joanne Clark, BAAS, HT(ASCP)CM Director of Histology