From MMargiotta at bmhmc.org Mon May 2 08:09:22 2016 From: MMargiotta at bmhmc.org (Margiotta-Watz, Michele) Date: Mon, 2 May 2016 13:09:22 +0000 Subject: [Histonet] open position Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC6DA283B4@BMH-EXCHANGE-01.BMHMC.ORG> Hi All, We have an open position for a Histotech since one of my techs is retiring. This is a full-time position at a small community Hospital on eastern Long Island, NY. We are looking for an experienced tech to work early morning hours Monday thru Friday and no holidays. If interested, please e-mail your resume to me at mmargiotta at bmhmc.org or call me at the # below. Thanks, Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Road Patchogue, NY 11772 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From mward at wakehealth.edu Mon May 2 09:03:58 2016 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Mon, 2 May 2016 14:03:58 +0000 Subject: [Histonet] PLA2R assay question In-Reply-To: References: Message-ID: I have been asked to look into offering PLA2R on our renal biopsies and in doing some research on the subject I find that some people are staining frozen tissue via an indirect IMF procedure and some are staining ffpe tissues, also using an indirect IMF procedure. I would prefer to do this via traditional IHC (we have Bond 2 stainers). Is anyone doing this way? If so, could you provide some tips on getting it to work successfully? Thanks in advance for any help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward at wakehealth.edu From LRaff at uropartners.com Mon May 2 12:39:28 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 2 May 2016 17:39:28 +0000 Subject: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10D5E354@COLOEXCH01.uropartners.local> To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From cjbulmer2526 at aol.com Mon May 2 12:42:36 2016 From: cjbulmer2526 at aol.com (Cindy Bulmer) Date: Mon, 2 May 2016 13:42:36 -0400 Subject: [Histonet] IHC positive controls Message-ID: <15472908bba-2169-bd52@webprd-a99.mail.aol.com> Hello Histoland, I need some advice, I have a PT block that is positive with Spirochetes. What would be the best way to use this block as a positive control? 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. then put slides in refrigerator for future use. 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides directly in refrigerator for future use. 3) Cut "fresh" every time they order the Ab. Thank you, Cindy Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX From rjbuesa at yahoo.com Mon May 2 13:22:59 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 2 May 2016 18:22:59 +0000 (UTC) Subject: [Histonet] No More Blog Posts -- Over and Out! In-Reply-To: <6347C6D2B080534F9B5C2B08436DCFAF10D5E354@COLOEXCH01.uropartners.local> References: <6347C6D2B080534F9B5C2B08436DCFAF10D5E354@COLOEXCH01.uropartners.local> Message-ID: <1946825210.5059374.1462213379630.JavaMail.yahoo@mail.yahoo.com> Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise.? Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lindamargraf at gmail.com Mon May 2 13:37:35 2016 From: lindamargraf at gmail.com (Linda Margraf) Date: Mon, 2 May 2016 13:37:35 -0500 Subject: [Histonet] Fwd: References: <15472779e30-2169-bb2e@webprd-a99.mail.aol.com> Message-ID: <31CF79C7-84C3-45B8-AF7B-20151C22F338@gmail.com> > From: Cindy Bulmer > Date: May 2, 2016 at 12:15:22 PM CDT > To: histonet-owner at lists.utsouthwestern.edu > > Hello Histoland, > > I need some advice, I have a PT block that is positive with Spirochetes. > What would be the best way to use this block as a positive control? > > 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. > then put slides in refrigerator for future use. > 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides > directly in refrigerator for future use. > 3) Cut "fresh" every time they order the Ab. > > Thank you, > Cindy > Cynthia Bulmer HT(ASCP),QIHC > IHC Supervisor, CTPL > Waco, TX From philip_manfre at merck.com Mon May 2 13:38:32 2016 From: philip_manfre at merck.com (Manfre, Philip) Date: Mon, 2 May 2016 14:38:32 -0400 Subject: [Histonet] No More Blog Posts -- Over and Out! In-Reply-To: <1946825210.5059374.1462213379630.JavaMail.yahoo@mail.yahoo.com> References: <6347C6D2B080534F9B5C2B08436DCFAF10D5E354@COLOEXCH01.uropartners.local> <1946825210.5059374.1462213379630.JavaMail.yahoo@mail.yahoo.com> Message-ID: <558A4571351D0C42BD923F403F4198C40111D88D1C5C@USCTMXP51014.merck.com> Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise.? Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Timothy.Morken at ucsf.edu Mon May 2 13:47:08 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 2 May 2016 18:47:08 +0000 Subject: [Histonet] IHC positive controls In-Reply-To: <15472908bba-2169-bd52@webprd-a99.mail.aol.com> References: <15472908bba-2169-bd52@webprd-a99.mail.aol.com> Message-ID: <761E2B5697F795489C8710BCC72141FF6FD47684@ex07.net.ucsf.edu> Cynthia, best practice is to keep in the block and cut as needed. Second is to cut and dry at room temp and not melt, then store. Don't cut, melt and store. The issue is oxidation. Keeping in paraffin prevents oxidation. You'll protect the antigens better if they are fully isolated from air, which paraffin does, but once cut, they are exposed. That happens in the fridge as well and I'm not sure refrigeration helps much. I'm guessing that you don't do a lot of these, so you don't want to cut a lot of slide and have them sitting around for months waiting for use. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cindy Bulmer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 10:43 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC positive controls Hello Histoland, I need some advice, I have a PT block that is positive with Spirochetes. What would be the best way to use this block as a positive control? 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. then put slides in refrigerator for future use. 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides directly in refrigerator for future use. 3) Cut "fresh" every time they order the Ab. Thank you, Cindy Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd at chs.net Mon May 2 14:08:22 2016 From: DKBoyd at chs.net (Boyd, Debbie M) Date: Mon, 2 May 2016 19:08:22 +0000 Subject: [Histonet] No More Blog Posts -- Over and Out! In-Reply-To: <558A4571351D0C42BD923F403F4198C40111D88D1C5C@USCTMXP51014.merck.com> References: <6347C6D2B080534F9B5C2B08436DCFAF10D5E354@COLOEXCH01.uropartners.local> <1946825210.5059374.1462213379630.JavaMail.yahoo@mail.yahoo.com>, <558A4571351D0C42BD923F403F4198C40111D88D1C5C@USCTMXP51014.merck.com> Message-ID: <7EAFE982E328304DA6CE2B677BB76246AB4B7465@TN001WEXMBX014.US.chs.net> I just feel if you didn't care for the subject/blog (doesn't pertain to you, you don't have any exposure to it etc. ) you could have deleted it without being so mean spirited. I have in the past ignored the mean spirited sparring, but this was a bit much. Whatever happened to kindness? Just my two cents.... Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Manfre, Philip via Histonet [histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:38 PM To: Rene J Buesa Cc: 'histonet at lists.utsouthwestern.edu' Subject: [EXTERNAL] Re: [Histonet] No More Blog Posts -- Over and Out! Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From wdesalvo.cac at outlook.com Mon May 2 14:10:44 2016 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Mon, 2 May 2016 12:10:44 -0700 Subject: [Histonet] No More Blog Posts -- Over and Out! Message-ID: Your response is WAY out of line. Keep it professional. Sent from my Windows Phone ________________________________ From: Manfre, Philip via Histonet Sent: ?5/?2/?2016 12:08 PM To: Rene J Buesa Cc: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mills at 3scan.com Mon May 2 16:49:25 2016 From: mills at 3scan.com (Caroline Miller) Date: Mon, 2 May 2016 14:49:25 -0700 Subject: [Histonet] No More Blog Posts -- Over and Out! In-Reply-To: <6347C6D2B080534F9B5C2B08436DCFAF10D5E354@COLOEXCH01.uropartners.local> References: <6347C6D2B080534F9B5C2B08436DCFAF10D5E354@COLOEXCH01.uropartners.local> Message-ID: Thank you Lester, I really appreciate your decision, and I look forward to reading your histology-related histonet posts. yours, mills On Mon, May 2, 2016 at 10:39 AM, Lester Raff MD via Histonet < histonet at lists.utsouthwestern.edu> wrote: > To My Lab Colleagues: > > As my intent has never been to sow discontent or rancor, I think it is for > the best if I no longer post links to my blog, lab related or otherwise. > Of course the blogs go on, and if anyone is interested in being added to my > mailing list for future notifications, just drop me a line at > les.raff at post.com The mailing list is never > used for any purpose other than announcing a new blog post. Be sure to let > me know you are from the Histonet list! > > I will continue to participate in any histology/pathologist discussions > here as I have for many years. > > Cheers, > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From wdesalvo.cac at outlook.com Mon May 2 17:49:09 2016 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Mon, 2 May 2016 15:49:09 -0700 Subject: [Histonet] No More Blog Posts -- Over and Out! In-Reply-To: References: , Message-ID: Another personal e-mail from you that is unwanted. I am feeling like I have my own stalker. You are one of the reasons that individuals will not post or comment, personal and ugly. I cannot imagine why your workplace (LUMC.edu) chose you to represent them. I request that you no longer contact me and make personal attacks. I know nothing of you and you certainly do not know anything about me. If you have something to say, post to the listserve, not me. > From: SPINHEIRO at lumc.edu > To: wdesalvo.cac at outlook.com > Subject: RE: [Histonet] No More Blog Posts -- Over and Out! > Date: Mon, 2 May 2016 20:15:38 +0000 > > As is yours. Just can't keep that dictatorial aspect out of your charming consultant two cents worth. > > Steve Pinheiro > > > -----Original Message----- > From: WILLIAM DESALVO via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Monday, May 02, 2016 2:11 PM > To: Manfre, Philip; Rene J Buesa > Cc: 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Your response is WAY out of line. Keep it professional. > > Sent from my Windows Phone > ________________________________ > From: Manfre, Philip via Histonet > Sent: ?5/?2/?2016 12:08 PM > To: Rene J Buesa > Cc: 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! > > -----Original Message----- > From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Monday, May 02, 2016 2:23 PM > To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? > > On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: > > > To My Lab Colleagues: > > As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! > > I will continue to participate in any histology/pathologist discussions here as I have for many years. > > Cheers, > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From rjbuesa at yahoo.com Tue May 3 08:28:28 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 3 May 2016 13:28:28 +0000 (UTC) Subject: [Histonet] Fwd: In-Reply-To: <31CF79C7-84C3-45B8-AF7B-20151C22F338@gmail.com> References: <31CF79C7-84C3-45B8-AF7B-20151C22F338@gmail.com> Message-ID: <1663136023.5351238.1462282108474.JavaMail.yahoo@mail.yahoo.com> As I see it, the best solution is "1"Even more: if the piece of tissue is large enough,?cut 1 section and stain ? select at least 2 (+) areas? divide the block into 2 blocks each containing one of those 2 areas?and?by doing so?you would have duplicated the number of possible (+) sections.Ren? On Monday, May 2, 2016 3:09 PM, Linda Margraf via Histonet wrote: > From: Cindy Bulmer > Date: May 2, 2016 at 12:15:22 PM CDT > To: histonet-owner at lists.utsouthwestern.edu > > Hello Histoland, >? > I need some advice, I have a PT block that is positive with Spirochetes. > What would be the best way to use this block as a positive control? >? > 1)? Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. >? ? ? then put slides in refrigerator for future use. > 2)? Cut (serial sections, stain the last slide for bugs) NO oven time? and put slides >? ? ? directly in refrigerator for future use. > 3)? Cut "fresh" every time they order the Ab. >? > Thank you, > Cindy > Cynthia Bulmer HT(ASCP),QIHC > IHC Supervisor, CTPL > Waco, TX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KSimeone at leavittmgt.com Tue May 3 11:29:18 2016 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Tue, 3 May 2016 16:29:18 +0000 Subject: [Histonet] HHV-8 RTU Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CC918550A@vm-email.leavittmgt.com> My vendor (Cell Marque/Sigma Aldrich) has a YEAR backorder on this IHC RTU. Does anyone have a vendor they purchase from that I can research? Thanks so much. Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From PAMarcum at uams.edu Tue May 3 11:39:16 2016 From: PAMarcum at uams.edu (Marcum, Pamela A) Date: Tue, 3 May 2016 16:39:16 +0000 Subject: [Histonet] PAS/Decal Question Message-ID: <826681eae65c40b28de6f620b3207f05@MAIL13M2N1.ad.uams.edu> We are still having issues with our PAS stain on decaled bone marrows. The Pathologists in HemePath are seeing what they refer to as smudginess in cells on some areas of the completed PAS slides. We have looked at everything and cannot find where the issue is coming from at this point. We have done manual staining for PAS, automated on the Leica stainer and on the Dako Artisan. All methods show the same result for some slides. We can go for several days to a week or more with no problem and then suddenly it is back and we have changed nothing in the way we do the processing, embedding, sectioning, deparaffinization and coverslipping. We do as many as 38 bone marrow cores a night or as few as 8 and can find no correlation in the number we have to deal with for a given period. All bone marrows drawn today must be completed by 8AM tomorrow morning. Fixation after pulling the bone marrows is a minimum of 2 hours in AZF with a maximum of 7 hours +/-. Grossed and placed in cassettes for 15 minute rinse in running DI Water Decal currently in the Milestone Decal Unit for 45 minutes in Immunocal at 50C Rinsed in running DI water for 15 minutes Placed in 10% NBF and processed on a 4 hour program with a delay of 4 hours minimum to come off at 4:45AM. If anyone knows of any literature on decal effects on PAS staining in bone marrows please contact me. This has been going on for months and no matter what we do manual staining, Leica adaptation for automated or Dako it is not helping. Dako has been great with sending in technical experts repeatedly and we cannot get this corrected. Thanks, Pam ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From paula at excaliburpathology.com Tue May 3 12:53:48 2016 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Tue, 3 May 2016 17:53:48 +0000 (UTC) Subject: [Histonet] Job Opening in CA References: <1916726542.7000662.1462298028301.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1916726542.7000662.1462298028301.JavaMail.yahoo@mail.yahoo.com> | | | | | | Dear Friends & Family,I am seeking a?Research?Scientist I, Stem Cell Biology?for a stem cell therapy project that we just?started. The funding has been?secured for the entire?project. The position is available?immediately and the description is shown?below.?If you know somebody who may be?interested?in this?opening, please share this e-mail with your?contact.?Any help, advice or recommendations are highly?appreciated?as always!Respectfully,Nikolay Turovets, Ph.D.CEO?MediCell Technologies, LLC | | | | | | | | | | | | RESEARCH SCIENTIST I, Stem Cell Biology | | | | Job Description Novel exclusive stem cell therapy project is seeking a highly motivated scientist to join its team.In consortium with a leading hospital in Los Angeles and a recognized business partner, MediCell Technologies is developing a novel stem cell-based therapeutic platform. The technology has been validated in animal proof-of-concept studies and attracts interests of key opinion leaders of the field. The funding has been secured for entire project.We are looking for a highly motivated and pro-active individual who is willing to grow with the project and contribute to the development of stem cell-based therapies. Our project is an outstanding opportunity for the right individual to display their talents and establish themselves in this highly competitive field.The scientist will be responsible for the derivation, expansion and characterization of a unique type of human stem cells. He or She will be involved in all range of activities associated with the technology transfer to a GMP facility as well as preparing a portfolio of Standard Operation Procedures. The Scientist will play an integral role in managing various aspects of lab operations-associated activities. The work will require periodic work on weekends, holidays and after business-hours time.This position requires hard work, ingenuity, and a strong commitment to a productive work environment. If you are not a highly confident, self-starting individual, this position is not meant for you. | | | | Required qualifications - PhD in cell biology, stem cell biology, tissue engineering or related discipline. 3+ years of work experience in stem cell research. - Outstanding hands-on techniques in stem cell culture, cell-based assays (including ICC, qPCR, FACS), and cell culture process development. - Excellent English written and oral communication skills, able to write SOPs, technical reports and protocols. - Demonstrated excellence in planning, executing, and analyzing experiments. - Able to troubleshoot, solve difficult problems, and develop process improvements. - Attention to detail and careful record-keeping. - Excellent organizational skills and ability to manage multiple projects. - Flexible team player excited to collaborate with internal and external partners. - Highly self-motivated. - Flexibility in working schedule to accommodate weekend/holiday and out-business hours work if necessary. - Authorized to legally work in US. Preferred (but not required) qualifications: - Experience with human pluripotent and/or mesenchymal stem cells is preferred. - Experience to work in GMP and/or GLP environment is?appreciated. Salary:?up to?$85,000/annual?+ health insuranceJob Location:?Carlsbad, CARe-location expense:?Not offeredEmployment term:?Full time, 3 month probation period | | | | | | | | | | | | HOW TO APPLYPlease send CV and Cover Letter to Nikolay Turovets, PhD:?nt at medicelltech.com | | ?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From bcdukes at lexhealth.org Tue May 3 12:57:01 2016 From: bcdukes at lexhealth.org (Blake Taylor) Date: Tue, 3 May 2016 17:57:01 +0000 Subject: [Histonet] No More Blog Posts -- Over and Out! Message-ID: I have been completely disappointed at the remarks and ugliness that I have seen lately on this post. I will be extremely unlikely to continue to use this list serve anymore due to the complete lack of professionalism and compassion that has been displayed of late. This blog post talk has certainly brought out the worst in people but it certainly is not the first time I've seen people be short, judgmental and rude on this site. It has been nice to see some people speak up and ask others to behave better but as usual it is the worst of the crowd that always seems to be the loudest. Blake Taylor Surgical Pathology -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Tuesday, May 03, 2016 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 150, Issue 2 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. No More Blog Posts -- Over and Out! (Lester Raff MD) 2. IHC positive controls (Cindy Bulmer) 3. Re: No More Blog Posts -- Over and Out! (Rene J Buesa) 4. Fwd: (Linda Margraf) 5. Re: No More Blog Posts -- Over and Out! (Manfre, Philip) 6. Re: IHC positive controls (Morken, Timothy) 7. Re: No More Blog Posts -- Over and Out! (Boyd, Debbie M) 8. Re: No More Blog Posts -- Over and Out! (WILLIAM DESALVO) 9. Re: No More Blog Posts -- Over and Out! (Caroline Miller) 10. Re: No More Blog Posts -- Over and Out! (WILLIAM DESALVO) 11. Re: Fwd: (Rene J Buesa) 12. HHV-8 RTU (Delray Beach Pathology Kari Simeone) 13. PAS/Decal Question (Marcum, Pamela A) ---------------------------------------------------------------------- Message: 1 Date: Mon, 2 May 2016 17:39:28 +0000 From: Lester Raff MD To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10D5E354 at COLOEXCH01.uropartners.local> Content-Type: text/plain; charset="us-ascii" To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ------------------------------ Message: 2 Date: Mon, 2 May 2016 13:42:36 -0400 From: Cindy Bulmer To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC positive controls Message-ID: <15472908bba-2169-bd52 at webprd-a99.mail.aol.com> Content-Type: text/plain; charset=utf-8 Hello Histoland, I need some advice, I have a PT block that is positive with Spirochetes. What would be the best way to use this block as a positive control? 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. then put slides in refrigerator for future use. 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides directly in refrigerator for future use. 3) Cut "fresh" every time they order the Ab. Thank you, Cindy Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX ------------------------------ Message: 3 Date: Mon, 2 May 2016 18:22:59 +0000 (UTC) From: Rene J Buesa To: Lester Raff MD , "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <1946825210.5059374.1462213379630.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise.? Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 2 May 2016 13:37:35 -0500 From: Linda Margraf To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fwd: Message-ID: <31CF79C7-84C3-45B8-AF7B-20151C22F338 at gmail.com> Content-Type: text/plain; charset=us-ascii > From: Cindy Bulmer > Date: May 2, 2016 at 12:15:22 PM CDT > To: histonet-owner at lists.utsouthwestern.edu > > Hello Histoland, > > I need some advice, I have a PT block that is positive with Spirochetes. > What would be the best way to use this block as a positive control? > > 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. > then put slides in refrigerator for future use. > 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides > directly in refrigerator for future use. > 3) Cut "fresh" every time they order the Ab. > > Thank you, > Cindy > Cynthia Bulmer HT(ASCP),QIHC > IHC Supervisor, CTPL > Waco, TX ------------------------------ Message: 5 Date: Mon, 2 May 2016 14:38:32 -0400 From: "Manfre, Philip" To: Rene J Buesa Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <558A4571351D0C42BD923F403F4198C40111D88D1C5C at USCTMXP51014.merck.com> Content-Type: text/plain; charset="utf-8" Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise.? Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 6 Date: Mon, 2 May 2016 18:47:08 +0000 From: "Morken, Timothy" To: Cindy Bulmer Cc: Histonet Subject: Re: [Histonet] IHC positive controls Message-ID: <761E2B5697F795489C8710BCC72141FF6FD47684 at ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Cynthia, best practice is to keep in the block and cut as needed. Second is to cut and dry at room temp and not melt, then store. Don't cut, melt and store. The issue is oxidation. Keeping in paraffin prevents oxidation. You'll protect the antigens better if they are fully isolated from air, which paraffin does, but once cut, they are exposed. That happens in the fridge as well and I'm not sure refrigeration helps much. I'm guessing that you don't do a lot of these, so you don't want to cut a lot of slide and have them sitting around for months waiting for use. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cindy Bulmer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 10:43 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC positive controls Hello Histoland, I need some advice, I have a PT block that is positive with Spirochetes. What would be the best way to use this block as a positive control? 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. then put slides in refrigerator for future use. 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides directly in refrigerator for future use. 3) Cut "fresh" every time they order the Ab. Thank you, Cindy Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 2 May 2016 19:08:22 +0000 From: "Boyd, Debbie M" To: "Manfre, Philip" Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <7EAFE982E328304DA6CE2B677BB76246AB4B7465 at TN001WEXMBX014.US.chs.net> Content-Type: text/plain; charset="utf-8" I just feel if you didn't care for the subject/blog (doesn't pertain to you, you don't have any exposure to it etc. ) you could have deleted it without being so mean spirited. I have in the past ignored the mean spirited sparring, but this was a bit much. Whatever happened to kindness? Just my two cents.... Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Manfre, Philip via Histonet [histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:38 PM To: Rene J Buesa Cc: 'histonet at lists.utsouthwestern.edu' Subject: [EXTERNAL] Re: [Histonet] No More Blog Posts -- Over and Out! Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 8 Date: Mon, 2 May 2016 12:10:44 -0700 From: WILLIAM DESALVO To: "Manfre, Philip" , Rene J Buesa Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: Content-Type: text/plain; charset="utf-8" Your response is WAY out of line. Keep it professional. Sent from my Windows Phone ________________________________ From: Manfre, Philip via Histonet Sent: ?5/?2/?2016 12:08 PM To: Rene J Buesa Cc: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 2 May 2016 14:49:25 -0700 From: Caroline Miller To: Lester Raff MD Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: Content-Type: text/plain; charset=UTF-8 Thank you Lester, I really appreciate your decision, and I look forward to reading your histology-related histonet posts. yours, mills On Mon, May 2, 2016 at 10:39 AM, Lester Raff MD via Histonet < histonet at lists.utsouthwestern.edu> wrote: > To My Lab Colleagues: > > As my intent has never been to sow discontent or rancor, I think it is for > the best if I no longer post links to my blog, lab related or otherwise. > Of course the blogs go on, and if anyone is interested in being added to my > mailing list for future notifications, just drop me a line at > les.raff at post.com The mailing list is never > used for any purpose other than announcing a new blog post. Be sure to let > me know you are from the Histonet list! > > I will continue to participate in any histology/pathologist discussions > here as I have for many years. > > Cheers, > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 10 Date: Mon, 2 May 2016 15:49:09 -0700 From: WILLIAM DESALVO To: STEVEN PINHEIRO Cc: Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: Content-Type: text/plain; charset="windows-1256" Another personal e-mail from you that is unwanted. I am feeling like I have my own stalker. You are one of the reasons that individuals will not post or comment, personal and ugly. I cannot imagine why your workplace (LUMC.edu) chose you to represent them. I request that you no longer contact me and make personal attacks. I know nothing of you and you certainly do not know anything about me. If you have something to say, post to the listserve, not me. > From: SPINHEIRO at lumc.edu > To: wdesalvo.cac at outlook.com > Subject: RE: [Histonet] No More Blog Posts -- Over and Out! > Date: Mon, 2 May 2016 20:15:38 +0000 > > As is yours. Just can't keep that dictatorial aspect out of your charming consultant two cents worth. > > Steve Pinheiro > > > -----Original Message----- > From: WILLIAM DESALVO via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Monday, May 02, 2016 2:11 PM > To: Manfre, Philip; Rene J Buesa > Cc: 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Your response is WAY out of line. Keep it professional. > > Sent from my Windows Phone > ________________________________ > From: Manfre, Philip via Histonet > Sent: ?5/?2/?2016 12:08 PM > To: Rene J Buesa > Cc: 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! > > -----Original Message----- > From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Monday, May 02, 2016 2:23 PM > To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? > > On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: > > > To My Lab Colleagues: > > As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! > > I will continue to participate in any histology/pathologist discussions here as I have for many years. > > Cheers, > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 11 Date: Tue, 3 May 2016 13:28:28 +0000 (UTC) From: Rene J Buesa To: Linda Margraf , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Fwd: Message-ID: <1663136023.5351238.1462282108474.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 As I see it, the best solution is "1"Even more: if the piece of tissue is large enough,?cut 1 section and stain ? select at least 2 (+) areas? divide the block into 2 blocks each containing one of those 2 areas?and?by doing so?you would have duplicated the number of possible (+) sections.Ren? On Monday, May 2, 2016 3:09 PM, Linda Margraf via Histonet wrote: > From: Cindy Bulmer > Date: May 2, 2016 at 12:15:22 PM CDT > To: histonet-owner at lists.utsouthwestern.edu > > Hello Histoland, >? > I need some advice, I have a PT block that is positive with Spirochetes. > What would be the best way to use this block as a positive control? >? > 1)? Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. >? ? ? then put slides in refrigerator for future use. > 2)? Cut (serial sections, stain the last slide for bugs) NO oven time? and put slides >? ? ? directly in refrigerator for future use. > 3)? Cut "fresh" every time they order the Ab. >? > Thank you, > Cindy > Cynthia Bulmer HT(ASCP),QIHC > IHC Supervisor, CTPL > Waco, TX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 3 May 2016 16:29:18 +0000 From: Delray Beach Pathology Kari Simeone To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] HHV-8 RTU Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CC918550A at vm-email.leavittmgt.com> Content-Type: text/plain; charset="iso-8859-1" My vendor (Cell Marque/Sigma Aldrich) has a YEAR backorder on this IHC RTU. Does anyone have a vendor they purchase from that I can research? Thanks so much. Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. ------------------------------ Message: 13 Date: Tue, 3 May 2016 16:39:16 +0000 From: "Marcum, Pamela A" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] PAS/Decal Question Message-ID: <826681eae65c40b28de6f620b3207f05 at MAIL13M2N1.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" We are still having issues with our PAS stain on decaled bone marrows. The Pathologists in HemePath are seeing what they refer to as smudginess in cells on some areas of the completed PAS slides. We have looked at everything and cannot find where the issue is coming from at this point. We have done manual staining for PAS, automated on the Leica stainer and on the Dako Artisan. All methods show the same result for some slides. We can go for several days to a week or more with no problem and then suddenly it is back and we have changed nothing in the way we do the processing, embedding, sectioning, deparaffinization and coverslipping. We do as many as 38 bone marrow cores a night or as few as 8 and can find no correlation in the number we have to deal with for a given period. All bone marrows drawn today must be completed by 8AM tomorrow morning. Fixation after pulling the bone marrows is a minimum of 2 hours in AZF with a maximum of 7 hours +/-. Grossed and placed in cassettes for 15 minute rinse in running DI Water Decal currently in the Milestone Decal Unit for 45 minutes in Immunocal at 50C Rinsed in running DI water for 15 minutes Placed in 10% NBF and processed on a 4 hour program with a delay of 4 hours minimum to come off at 4:45AM. If anyone knows of any literature on decal effects on PAS staining in bone marrows please contact me. This has been going on for months and no matter what we do manual staining, Leica adaptation for automated or Dako it is not helping. Dako has been great with sending in technical experts repeatedly and we cannot get this corrected. Thanks, Pam ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 150, Issue 2 **************************************** PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. From mwerdler at gmail.com Tue May 3 13:42:28 2016 From: mwerdler at gmail.com (Mca Werdler) Date: Tue, 3 May 2016 13:42:28 -0500 Subject: [Histonet] Golgi Kopsch Message-ID: Hello Everyone, I am completely new to the technique, i tried several different protocols but it doesn't seemed to succeed that very well. Does anyone have a protocol for the Golgi Kopsch, where i have to include the tissue in the end with paraffin? Thank you all for your time Mwerdler UNAM Mexico From thigginsht at msn.com Tue May 3 15:03:40 2016 From: thigginsht at msn.com (T H) Date: Tue, 3 May 2016 20:03:40 +0000 Subject: [Histonet] Histonet Digest, Vol 150, Issue 2 In-Reply-To: References: Message-ID: Good to know that I am not the only on that think Rene is negative to others. Hey Rene it's not just one person that thinks your a dirt bag. ________________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, May 3, 2016 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 150, Issue 2 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. No More Blog Posts -- Over and Out! (Lester Raff MD) 2. IHC positive controls (Cindy Bulmer) 3. Re: No More Blog Posts -- Over and Out! (Rene J Buesa) 4. Fwd: (Linda Margraf) 5. Re: No More Blog Posts -- Over and Out! (Manfre, Philip) 6. Re: IHC positive controls (Morken, Timothy) 7. Re: No More Blog Posts -- Over and Out! (Boyd, Debbie M) 8. Re: No More Blog Posts -- Over and Out! (WILLIAM DESALVO) 9. Re: No More Blog Posts -- Over and Out! (Caroline Miller) 10. Re: No More Blog Posts -- Over and Out! (WILLIAM DESALVO) 11. Re: Fwd: (Rene J Buesa) 12. HHV-8 RTU (Delray Beach Pathology Kari Simeone) 13. PAS/Decal Question (Marcum, Pamela A) ---------------------------------------------------------------------- Message: 1 Date: Mon, 2 May 2016 17:39:28 +0000 From: Lester Raff MD To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10D5E354 at COLOEXCH01.uropartners.local> Content-Type: text/plain; charset="us-ascii" To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ------------------------------ Message: 2 Date: Mon, 2 May 2016 13:42:36 -0400 From: Cindy Bulmer To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC positive controls Message-ID: <15472908bba-2169-bd52 at webprd-a99.mail.aol.com> Content-Type: text/plain; charset=utf-8 Hello Histoland, I need some advice, I have a PT block that is positive with Spirochetes. What would be the best way to use this block as a positive control? 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. then put slides in refrigerator for future use. 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides directly in refrigerator for future use. 3) Cut "fresh" every time they order the Ab. Thank you, Cindy Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX ------------------------------ Message: 3 Date: Mon, 2 May 2016 18:22:59 +0000 (UTC) From: Rene J Buesa To: Lester Raff MD , "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <1946825210.5059374.1462213379630.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise.? Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 2 May 2016 13:37:35 -0500 From: Linda Margraf To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fwd: Message-ID: <31CF79C7-84C3-45B8-AF7B-20151C22F338 at gmail.com> Content-Type: text/plain; charset=us-ascii > From: Cindy Bulmer > Date: May 2, 2016 at 12:15:22 PM CDT > To: histonet-owner at lists.utsouthwestern.edu > > Hello Histoland, > > I need some advice, I have a PT block that is positive with Spirochetes. > What would be the best way to use this block as a positive control? > > 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. > then put slides in refrigerator for future use. > 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides > directly in refrigerator for future use. > 3) Cut "fresh" every time they order the Ab. > > Thank you, > Cindy > Cynthia Bulmer HT(ASCP),QIHC > IHC Supervisor, CTPL > Waco, TX ------------------------------ Message: 5 Date: Mon, 2 May 2016 14:38:32 -0400 From: "Manfre, Philip" To: Rene J Buesa Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <558A4571351D0C42BD923F403F4198C40111D88D1C5C at USCTMXP51014.merck.com> Content-Type: text/plain; charset="utf-8" Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise.? Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 6 Date: Mon, 2 May 2016 18:47:08 +0000 From: "Morken, Timothy" To: Cindy Bulmer Cc: Histonet Subject: Re: [Histonet] IHC positive controls Message-ID: <761E2B5697F795489C8710BCC72141FF6FD47684 at ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Cynthia, best practice is to keep in the block and cut as needed. Second is to cut and dry at room temp and not melt, then store. Don't cut, melt and store. The issue is oxidation. Keeping in paraffin prevents oxidation. You'll protect the antigens better if they are fully isolated from air, which paraffin does, but once cut, they are exposed. That happens in the fridge as well and I'm not sure refrigeration helps much. I'm guessing that you don't do a lot of these, so you don't want to cut a lot of slide and have them sitting around for months waiting for use. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cindy Bulmer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 10:43 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC positive controls Hello Histoland, I need some advice, I have a PT block that is positive with Spirochetes. What would be the best way to use this block as a positive control? 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. then put slides in refrigerator for future use. 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides directly in refrigerator for future use. 3) Cut "fresh" every time they order the Ab. Thank you, Cindy Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 2 May 2016 19:08:22 +0000 From: "Boyd, Debbie M" To: "Manfre, Philip" Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <7EAFE982E328304DA6CE2B677BB76246AB4B7465 at TN001WEXMBX014.US.chs.net> Content-Type: text/plain; charset="utf-8" I just feel if you didn't care for the subject/blog (doesn't pertain to you, you don't have any exposure to it etc. ) you could have deleted it without being so mean spirited. I have in the past ignored the mean spirited sparring, but this was a bit much. Whatever happened to kindness? Just my two cents.... Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Manfre, Philip via Histonet [histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:38 PM To: Rene J Buesa Cc: 'histonet at lists.utsouthwestern.edu' Subject: [EXTERNAL] Re: [Histonet] No More Blog Posts -- Over and Out! Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 8 Date: Mon, 2 May 2016 12:10:44 -0700 From: WILLIAM DESALVO To: "Manfre, Philip" , Rene J Buesa Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: Content-Type: text/plain; charset="utf-8" Your response is WAY out of line. Keep it professional. Sent from my Windows Phone ________________________________ From: Manfre, Philip via Histonet Sent: ?5/?2/?2016 12:08 PM To: Rene J Buesa Cc: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 2 May 2016 14:49:25 -0700 From: Caroline Miller To: Lester Raff MD Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: Content-Type: text/plain; charset=UTF-8 Thank you Lester, I really appreciate your decision, and I look forward to reading your histology-related histonet posts. yours, mills On Mon, May 2, 2016 at 10:39 AM, Lester Raff MD via Histonet < histonet at lists.utsouthwestern.edu> wrote: > To My Lab Colleagues: > > As my intent has never been to sow discontent or rancor, I think it is for > the best if I no longer post links to my blog, lab related or otherwise. > Of course the blogs go on, and if anyone is interested in being added to my > mailing list for future notifications, just drop me a line at > les.raff at post.com The mailing list is never > used for any purpose other than announcing a new blog post. Be sure to let > me know you are from the Histonet list! > > I will continue to participate in any histology/pathologist discussions > here as I have for many years. > > Cheers, > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 10 Date: Mon, 2 May 2016 15:49:09 -0700 From: WILLIAM DESALVO To: STEVEN PINHEIRO Cc: Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: Content-Type: text/plain; charset="windows-1256" Another personal e-mail from you that is unwanted. I am feeling like I have my own stalker. You are one of the reasons that individuals will not post or comment, personal and ugly. I cannot imagine why your workplace (LUMC.edu) chose you to represent them. I request that you no longer contact me and make personal attacks. I know nothing of you and you certainly do not know anything about me. If you have something to say, post to the listserve, not me. > From: SPINHEIRO at lumc.edu > To: wdesalvo.cac at outlook.com > Subject: RE: [Histonet] No More Blog Posts -- Over and Out! > Date: Mon, 2 May 2016 20:15:38 +0000 > > As is yours. Just can't keep that dictatorial aspect out of your charming consultant two cents worth. > > Steve Pinheiro > > > -----Original Message----- > From: WILLIAM DESALVO via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Monday, May 02, 2016 2:11 PM > To: Manfre, Philip; Rene J Buesa > Cc: 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Your response is WAY out of line. Keep it professional. > > Sent from my Windows Phone > ________________________________ > From: Manfre, Philip via Histonet > Sent: ?5/?2/?2016 12:08 PM > To: Rene J Buesa > Cc: 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! > > -----Original Message----- > From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Monday, May 02, 2016 2:23 PM > To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? > > On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: > > > To My Lab Colleagues: > > As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! > > I will continue to participate in any histology/pathologist discussions here as I have for many years. > > Cheers, > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 11 Date: Tue, 3 May 2016 13:28:28 +0000 (UTC) From: Rene J Buesa To: Linda Margraf , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Fwd: Message-ID: <1663136023.5351238.1462282108474.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 As I see it, the best solution is "1"Even more: if the piece of tissue is large enough,?cut 1 section and stain ? select at least 2 (+) areas? divide the block into 2 blocks each containing one of those 2 areas?and?by doing so?you would have duplicated the number of possible (+) sections.Ren? On Monday, May 2, 2016 3:09 PM, Linda Margraf via Histonet wrote: > From: Cindy Bulmer > Date: May 2, 2016 at 12:15:22 PM CDT > To: histonet-owner at lists.utsouthwestern.edu > > Hello Histoland, >? > I need some advice, I have a PT block that is positive with Spirochetes. > What would be the best way to use this block as a positive control? >? > 1)? Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. >? ? ? then put slides in refrigerator for future use. > 2)? Cut (serial sections, stain the last slide for bugs) NO oven time? and put slides >? ? ? directly in refrigerator for future use. > 3)? Cut "fresh" every time they order the Ab. >? > Thank you, > Cindy > Cynthia Bulmer HT(ASCP),QIHC > IHC Supervisor, CTPL > Waco, TX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 3 May 2016 16:29:18 +0000 From: Delray Beach Pathology Kari Simeone To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] HHV-8 RTU Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CC918550A at vm-email.leavittmgt.com> Content-Type: text/plain; charset="iso-8859-1" My vendor (Cell Marque/Sigma Aldrich) has a YEAR backorder on this IHC RTU. Does anyone have a vendor they purchase from that I can research? Thanks so much. Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. ------------------------------ Message: 13 Date: Tue, 3 May 2016 16:39:16 +0000 From: "Marcum, Pamela A" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] PAS/Decal Question Message-ID: <826681eae65c40b28de6f620b3207f05 at MAIL13M2N1.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" We are still having issues with our PAS stain on decaled bone marrows. The Pathologists in HemePath are seeing what they refer to as smudginess in cells on some areas of the completed PAS slides. We have looked at everything and cannot find where the issue is coming from at this point. We have done manual staining for PAS, automated on the Leica stainer and on the Dako Artisan. All methods show the same result for some slides. We can go for several days to a week or more with no problem and then suddenly it is back and we have changed nothing in the way we do the processing, embedding, sectioning, deparaffinization and coverslipping. We do as many as 38 bone marrow cores a night or as few as 8 and can find no correlation in the number we have to deal with for a given period. All bone marrows drawn today must be completed by 8AM tomorrow morning. Fixation after pulling the bone marrows is a minimum of 2 hours in AZF with a maximum of 7 hours +/-. Grossed and placed in cassettes for 15 minute rinse in running DI Water Decal currently in the Milestone Decal Unit for 45 minutes in Immunocal at 50C Rinsed in running DI water for 15 minutes Placed in 10% NBF and processed on a 4 hour program with a delay of 4 hours minimum to come off at 4:45AM. If anyone knows of any literature on decal effects on PAS staining in bone marrows please contact me. This has been going on for months and no matter what we do manual staining, Leica adaptation for automated or Dako it is not helping. Dako has been great with sending in technical experts repeatedly and we cannot get this corrected. Thanks, Pam ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 150, Issue 2 **************************************** From rjbuesa at yahoo.com Tue May 3 15:36:37 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 3 May 2016 20:36:37 +0000 (UTC) Subject: [Histonet] PAS/Decal Question In-Reply-To: <826681eae65c40b28de6f620b3207f05@MAIL13M2N1.ad.uams.edu> References: <826681eae65c40b28de6f620b3207f05@MAIL13M2N1.ad.uams.edu> Message-ID: <1643330668.5627765.1462307797073.JavaMail.yahoo@mail.yahoo.com> My impression is that your problem is during the decalcification step. It cannot be hurried and has to be in EDTA at pH 7All reagents have to be?prepared in pH7 phosphate buffer.The inconsistency resides in the fact that not all core Bx are the same regarding thickness, tissue condition or size.Besides you are hurrying too much. As yourself (and your pathologists) the following question: what good you take out of your protocol if the "failure" rate is as big as you describe?Change to EDTA and process more time.Ren? On Tuesday, May 3, 2016 1:01 PM, "Marcum, Pamela A via Histonet" wrote: We are still having issues with our PAS stain on decaled bone marrows.? The Pathologists in HemePath are seeing what they refer to as smudginess in cells on some areas of the completed PAS slides.? We have looked at everything and cannot find where the issue is coming from at this point.? We have done manual staining for PAS, automated on the Leica stainer and on the Dako Artisan.? All methods show the same result for some slides.? We can go for several days to a week or more with no problem and then suddenly it is back and we have changed nothing in the way we do the processing, embedding, sectioning, deparaffinization and coverslipping.? We do as many as 38 bone marrow cores a night or as few as 8 and can find no correlation in the number we have to deal with for a given period.? All bone marrows drawn today must be completed by 8AM tomorrow morning. Fixation after pulling the bone marrows is a minimum of 2 hours in AZF with a maximum of 7 hours +/-. Grossed and placed in cassettes for 15 minute rinse in running DI Water Decal currently in the Milestone Decal Unit for 45 minutes in Immunocal at 50C Rinsed in running DI water for 15 minutes Placed in 10% NBF and processed on a 4 hour program with a delay of 4 hours minimum to come off at 4:45AM. If anyone knows of any literature on decal effects on PAS staining in bone marrows please contact me.? This has been going on for months and no matter what we do manual staining, Leica adaptation for automated or Dako it is not helping.? Dako has been great with sending in technical experts repeatedly and we cannot get this corrected. Thanks, Pam ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gmartin at marshallmedical.org Tue May 3 15:45:59 2016 From: gmartin at marshallmedical.org (Martin, Gary) Date: Tue, 3 May 2016 13:45:59 -0700 Subject: [Histonet] Cryostat Chirp Message-ID: <6ED9D4252F278841A0593D3D788AF24C1CFE9834@mailsvr.MARSHMED.local> My Leica CM 1850 cryostat is making a chirping sound and I can't figure out what is doing this. Has anyone experienced this sound, if what was the resolve. Thanks Gary From liz at premierlab.com Tue May 3 15:58:20 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Tue, 3 May 2016 14:58:20 -0600 Subject: [Histonet] Cryostat Chirp In-Reply-To: <6ED9D4252F278841A0593D3D788AF24C1CFE9834@mailsvr.MARSHMED.local> References: <6ED9D4252F278841A0593D3D788AF24C1CFE9834@mailsvr.MARSHMED.local> Message-ID: <14E2C6176416974295479C64A11CB9AE02BEC2AD5EBF@SBS2K8.premierlab.local> Gary I think that might have to do with the fan motor inside the unit, it might need to be replaced. We had ours replaced a few years ago. Do you service/PM the cryostat yearly? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Martin, Gary via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 03, 2016 2:46 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cryostat Chirp My Leica CM 1850 cryostat is making a chirping sound and I can't figure out what is doing this. Has anyone experienced this sound, if what was the resolve. Thanks Gary _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pamela.Hudson at va.gov Wed May 4 06:01:00 2016 From: Pamela.Hudson at va.gov (Hudson, Pamela R ASHVAMC) Date: Wed, 4 May 2016 07:01:00 -0400 Subject: [Histonet] FW: [EXTERNAL] Re: Histonet Digest, Vol 150, Issue 2 Message-ID: <059D0B145C11034C8C19F60C1B008BA70CDE972D@VHAV06MSGA1.v06.med.va.gov> OMG This is terrible. I can't believe I belong to a group of such unprofessional people. Pamela R. Hudson, HT (ASCP) Charles George VA Medical Center Pathology Department 1100 Tunnel Road Asheville, NC 28805 828-298-7911 x5747 Pamela.Hudson at va.gov -----Original Message----- From: T H via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 03, 2016 4:04 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Histonet Digest, Vol 150, Issue 2 Good to know that I am not the only on that think Rene is negative to others. Hey Rene it's not just one person that thinks your a dirt bag. ________________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, May 3, 2016 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 150, Issue 2 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. No More Blog Posts -- Over and Out! (Lester Raff MD) 2. IHC positive controls (Cindy Bulmer) 3. Re: No More Blog Posts -- Over and Out! (Rene J Buesa) 4. Fwd: (Linda Margraf) 5. Re: No More Blog Posts -- Over and Out! (Manfre, Philip) 6. Re: IHC positive controls (Morken, Timothy) 7. Re: No More Blog Posts -- Over and Out! (Boyd, Debbie M) 8. Re: No More Blog Posts -- Over and Out! (WILLIAM DESALVO) 9. Re: No More Blog Posts -- Over and Out! (Caroline Miller) 10. Re: No More Blog Posts -- Over and Out! (WILLIAM DESALVO) 11. Re: Fwd: (Rene J Buesa) 12. HHV-8 RTU (Delray Beach Pathology Kari Simeone) 13. PAS/Decal Question (Marcum, Pamela A) ---------------------------------------------------------------------- Message: 1 Date: Mon, 2 May 2016 17:39:28 +0000 From: Lester Raff MD To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10D5E354 at COLOEXCH01.uropartners.local> Content-Type: text/plain; charset="us-ascii" To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ------------------------------ Message: 2 Date: Mon, 2 May 2016 13:42:36 -0400 From: Cindy Bulmer To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC positive controls Message-ID: <15472908bba-2169-bd52 at webprd-a99.mail.aol.com> Content-Type: text/plain; charset=utf-8 Hello Histoland, I need some advice, I have a PT block that is positive with Spirochetes. What would be the best way to use this block as a positive control? 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. then put slides in refrigerator for future use. 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides directly in refrigerator for future use. 3) Cut "fresh" every time they order the Ab. Thank you, Cindy Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX ------------------------------ Message: 3 Date: Mon, 2 May 2016 18:22:59 +0000 (UTC) From: Rene J Buesa To: Lester Raff MD , "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <1946825210.5059374.1462213379630.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise.? Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 2 May 2016 13:37:35 -0500 From: Linda Margraf To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fwd: Message-ID: <31CF79C7-84C3-45B8-AF7B-20151C22F338 at gmail.com> Content-Type: text/plain; charset=us-ascii > From: Cindy Bulmer > Date: May 2, 2016 at 12:15:22 PM CDT > To: histonet-owner at lists.utsouthwestern.edu > > Hello Histoland, > > I need some advice, I have a PT block that is positive with Spirochetes. > What would be the best way to use this block as a positive control? > > 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. > then put slides in refrigerator for future use. > 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides > directly in refrigerator for future use. > 3) Cut "fresh" every time they order the Ab. > > Thank you, > Cindy > Cynthia Bulmer HT(ASCP),QIHC > IHC Supervisor, CTPL > Waco, TX ------------------------------ Message: 5 Date: Mon, 2 May 2016 14:38:32 -0400 From: "Manfre, Philip" To: Rene J Buesa Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <558A4571351D0C42BD923F403F4198C40111D88D1C5C at USCTMXP51014.merck.com> Content-Type: text/plain; charset="utf-8" Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise.? Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 6 Date: Mon, 2 May 2016 18:47:08 +0000 From: "Morken, Timothy" To: Cindy Bulmer Cc: Histonet Subject: Re: [Histonet] IHC positive controls Message-ID: <761E2B5697F795489C8710BCC72141FF6FD47684 at ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Cynthia, best practice is to keep in the block and cut as needed. Second is to cut and dry at room temp and not melt, then store. Don't cut, melt and store. The issue is oxidation. Keeping in paraffin prevents oxidation. You'll protect the antigens better if they are fully isolated from air, which paraffin does, but once cut, they are exposed. That happens in the fridge as well and I'm not sure refrigeration helps much. I'm guessing that you don't do a lot of these, so you don't want to cut a lot of slide and have them sitting around for months waiting for use. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cindy Bulmer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 10:43 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC positive controls Hello Histoland, I need some advice, I have a PT block that is positive with Spirochetes. What would be the best way to use this block as a positive control? 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. then put slides in refrigerator for future use. 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides directly in refrigerator for future use. 3) Cut "fresh" every time they order the Ab. Thank you, Cindy Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 2 May 2016 19:08:22 +0000 From: "Boyd, Debbie M" To: "Manfre, Philip" Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <7EAFE982E328304DA6CE2B677BB76246AB4B7465 at TN001WEXMBX014.US.chs.net> Content-Type: text/plain; charset="utf-8" I just feel if you didn't care for the subject/blog (doesn't pertain to you, you don't have any exposure to it etc. ) you could have deleted it without being so mean spirited. I have in the past ignored the mean spirited sparring, but this was a bit much. Whatever happened to kindness? Just my two cents.... Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Manfre, Philip via Histonet [histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:38 PM To: Rene J Buesa Cc: 'histonet at lists.utsouthwestern.edu' Subject: [EXTERNAL] Re: [Histonet] No More Blog Posts -- Over and Out! Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 8 Date: Mon, 2 May 2016 12:10:44 -0700 From: WILLIAM DESALVO To: "Manfre, Philip" , Rene J Buesa Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: Content-Type: text/plain; charset="utf-8" Your response is WAY out of line. Keep it professional. Sent from my Windows Phone ________________________________ From: Manfre, Philip via Histonet Sent: ?5/?2/?2016 12:08 PM To: Rene J Buesa Cc: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 2 May 2016 14:49:25 -0700 From: Caroline Miller To: Lester Raff MD Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: Content-Type: text/plain; charset=UTF-8 Thank you Lester, I really appreciate your decision, and I look forward to reading your histology-related histonet posts. yours, mills On Mon, May 2, 2016 at 10:39 AM, Lester Raff MD via Histonet < histonet at lists.utsouthwestern.edu> wrote: > To My Lab Colleagues: > > As my intent has never been to sow discontent or rancor, I think it is > for the best if I no longer post links to my blog, lab related or otherwise. > Of course the blogs go on, and if anyone is interested in being added > to my mailing list for future notifications, just drop me a line at > les.raff at post.com The mailing list is never > used for any purpose other than announcing a new blog post. Be sure to > let me know you are from the Histonet list! > > I will continue to participate in any histology/pathologist > discussions here as I have for many years. > > Cheers, > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 10 Date: Mon, 2 May 2016 15:49:09 -0700 From: WILLIAM DESALVO To: STEVEN PINHEIRO Cc: Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: Content-Type: text/plain; charset="windows-1256" Another personal e-mail from you that is unwanted. I am feeling like I have my own stalker. You are one of the reasons that individuals will not post or comment, personal and ugly. I cannot imagine why your workplace (LUMC.edu) chose you to represent them. I request that you no longer contact me and make personal attacks. I know nothing of you and you certainly do not know anything about me. If you have something to say, post to the listserve, not me. > From: SPINHEIRO at lumc.edu > To: wdesalvo.cac at outlook.com > Subject: RE: [Histonet] No More Blog Posts -- Over and Out! > Date: Mon, 2 May 2016 20:15:38 +0000 > > As is yours. Just can't keep that dictatorial aspect out of your charming consultant two cents worth. > > Steve Pinheiro > > > -----Original Message----- > From: WILLIAM DESALVO via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > Sent: Monday, May 02, 2016 2:11 PM > To: Manfre, Philip; Rene J Buesa > Cc: 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Your response is WAY out of line. Keep it professional. > > Sent from my Windows Phone > ________________________________ > From: Manfre, Philip via > Histonet > Sent: ?5/?2/?2016 12:08 PM > To: Rene J Buesa > Cc: > 'histonet at lists.utsouthwestern.edu' rn.edu> > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! > > -----Original Message----- > From: Rene J Buesa via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > Sent: Monday, May 02, 2016 2:23 PM > To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? > > On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: > > > To My Lab Colleagues: > > As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! > > I will continue to participate in any histology/pathologist discussions here as I have for many years. > > Cheers, > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, > Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct > contact information for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 11 Date: Tue, 3 May 2016 13:28:28 +0000 (UTC) From: Rene J Buesa To: Linda Margraf , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Fwd: Message-ID: <1663136023.5351238.1462282108474.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 As I see it, the best solution is "1"Even more: if the piece of tissue is large enough,?cut 1 section and stain ? select at least 2 (+) areas? divide the block into 2 blocks each containing one of those 2 areas?and?by doing so?you would have duplicated the number of possible (+) sections.Ren? On Monday, May 2, 2016 3:09 PM, Linda Margraf via Histonet wrote: > From: Cindy Bulmer > Date: May 2, 2016 at 12:15:22 PM CDT > To: histonet-owner at lists.utsouthwestern.edu > > Hello Histoland, >? > I need some advice, I have a PT block that is positive with Spirochetes. > What would be the best way to use this block as a positive control? >? > 1)? Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. >? ? ? then put slides in refrigerator for future use. > 2)? Cut (serial sections, stain the last slide for bugs) NO oven time? >and put slides ? ? ? directly in refrigerator for future use. > 3)? Cut "fresh" every time they order the Ab. >? > Thank you, > Cindy > Cynthia Bulmer HT(ASCP),QIHC > IHC Supervisor, CTPL > Waco, TX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 3 May 2016 16:29:18 +0000 From: Delray Beach Pathology Kari Simeone To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] HHV-8 RTU Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CC918550A at vm-email.leavittmgt.com> Content-Type: text/plain; charset="iso-8859-1" My vendor (Cell Marque/Sigma Aldrich) has a YEAR backorder on this IHC RTU. Does anyone have a vendor they purchase from that I can research? Thanks so much. Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. ------------------------------ Message: 13 Date: Tue, 3 May 2016 16:39:16 +0000 From: "Marcum, Pamela A" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] PAS/Decal Question Message-ID: <826681eae65c40b28de6f620b3207f05 at MAIL13M2N1.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" We are still having issues with our PAS stain on decaled bone marrows. The Pathologists in HemePath are seeing what they refer to as smudginess in cells on some areas of the completed PAS slides. We have looked at everything and cannot find where the issue is coming from at this point. We have done manual staining for PAS, automated on the Leica stainer and on the Dako Artisan. All methods show the same result for some slides. We can go for several days to a week or more with no problem and then suddenly it is back and we have changed nothing in the way we do the processing, embedding, sectioning, deparaffinization and coverslipping. We do as many as 38 bone marrow cores a night or as few as 8 and can find no correlation in the number we have to deal with for a given period. All bone marrows drawn today must be completed by 8AM tomorrow morning. Fixation after pulling the bone marrows is a minimum of 2 hours in AZF with a maximum of 7 hours +/-. Grossed and placed in cassettes for 15 minute rinse in running DI Water Decal currently in the Milestone Decal Unit for 45 minutes in Immunocal at 50C Rinsed in running DI water for 15 minutes Placed in 10% NBF and processed on a 4 hour program with a delay of 4 hours minimum to come off at 4:45AM. If anyone knows of any literature on decal effects on PAS staining in bone marrows please contact me. This has been going on for months and no matter what we do manual staining, Leica adaptation for automated or Dako it is not helping. Dako has been great with sending in technical experts repeatedly and we cannot get this corrected. Thanks, Pam ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 150, Issue 2 **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting at geisinger.edu Wed May 4 06:25:01 2016 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Wed, 4 May 2016 11:25:01 +0000 Subject: [Histonet] Histonet Digest, Vol 150, Issue 2 In-Reply-To: <059D0B145C11034C8C19F60C1B008BA70CDE972D@VHAV06MSGA1.v06.med.va.gov> References: <059D0B145C11034C8C19F60C1B008BA70CDE972D@VHAV06MSGA1.v06.med.va.gov> Message-ID: <8d9427907067467bb6ea960ad5bcfcd8@LOFEXMBX108W12V.geisinger.edu> This seems to be the trend of society in general these days. No manners. -----Original Message----- From: Hudson, Pamela R ASHVAMC via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, May 04, 2016 7:01 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] FW: [EXTERNAL] Re: Histonet Digest, Vol 150, Issue 2 OMG This is terrible. I can't believe I belong to a group of such unprofessional people. Pamela R. Hudson, HT (ASCP) Charles George VA Medical Center Pathology Department 1100 Tunnel Road Asheville, NC 28805 828-298-7911 x5747 Pamela.Hudson at va.gov -----Original Message----- From: T H via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 03, 2016 4:04 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Histonet Digest, Vol 150, Issue 2 Good to know that I am not the only on that think Rene is negative to others. Hey Rene it's not just one person that thinks your a dirt bag. ________________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, May 3, 2016 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 150, Issue 2 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3aVNXN%2fZov8%3d or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. No More Blog Posts -- Over and Out! (Lester Raff MD) 2. IHC positive controls (Cindy Bulmer) 3. Re: No More Blog Posts -- Over and Out! (Rene J Buesa) 4. Fwd: (Linda Margraf) 5. Re: No More Blog Posts -- Over and Out! (Manfre, Philip) 6. Re: IHC positive controls (Morken, Timothy) 7. Re: No More Blog Posts -- Over and Out! (Boyd, Debbie M) 8. Re: No More Blog Posts -- Over and Out! (WILLIAM DESALVO) 9. Re: No More Blog Posts -- Over and Out! (Caroline Miller) 10. Re: No More Blog Posts -- Over and Out! (WILLIAM DESALVO) 11. Re: Fwd: (Rene J Buesa) 12. HHV-8 RTU (Delray Beach Pathology Kari Simeone) 13. PAS/Decal Question (Marcum, Pamela A) ---------------------------------------------------------------------- Message: 1 Date: Mon, 2 May 2016 17:39:28 +0000 From: Lester Raff MD To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10D5E354 at COLOEXCH01.uropartners.local> Content-Type: text/plain; charset="us-ascii" To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ------------------------------ Message: 2 Date: Mon, 2 May 2016 13:42:36 -0400 From: Cindy Bulmer To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC positive controls Message-ID: <15472908bba-2169-bd52 at webprd-a99.mail.aol.com> Content-Type: text/plain; charset=utf-8 Hello Histoland, I need some advice, I have a PT block that is positive with Spirochetes. What would be the best way to use this block as a positive control? 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. then put slides in refrigerator for future use. 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides directly in refrigerator for future use. 3) Cut "fresh" every time they order the Ab. Thank you, Cindy Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX ------------------------------ Message: 3 Date: Mon, 2 May 2016 18:22:59 +0000 (UTC) From: Rene J Buesa To: Lester Raff MD , "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <1946825210.5059374.1462213379630.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise.? Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3aVNXN%2fZov8%3d ------------------------------ Message: 4 Date: Mon, 2 May 2016 13:37:35 -0500 From: Linda Margraf To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fwd: Message-ID: <31CF79C7-84C3-45B8-AF7B-20151C22F338 at gmail.com> Content-Type: text/plain; charset=us-ascii > From: Cindy Bulmer > Date: May 2, 2016 at 12:15:22 PM CDT > To: histonet-owner at lists.utsouthwestern.edu > > Hello Histoland, > > I need some advice, I have a PT block that is positive with Spirochetes. > What would be the best way to use this block as a positive control? > > 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. > then put slides in refrigerator for future use. > 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides > directly in refrigerator for future use. > 3) Cut "fresh" every time they order the Ab. > > Thank you, > Cindy > Cynthia Bulmer HT(ASCP),QIHC > IHC Supervisor, CTPL > Waco, TX ------------------------------ Message: 5 Date: Mon, 2 May 2016 14:38:32 -0400 From: "Manfre, Philip" To: Rene J Buesa Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <558A4571351D0C42BD923F403F4198C40111D88D1C5C at USCTMXP51014.merck.com> Content-Type: text/plain; charset="utf-8" Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise.? Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3aVNXN%2fZov8%3d _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3aVNXN%2fZov8%3d Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2fwww.merck.com%2fcontact%2fcontacts.html&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=KCoctVheTDqWPFsXArnqT%2b%2fbL9lv0ACuqebB8qSnaU0%3d) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 6 Date: Mon, 2 May 2016 18:47:08 +0000 From: "Morken, Timothy" To: Cindy Bulmer Cc: Histonet Subject: Re: [Histonet] IHC positive controls Message-ID: <761E2B5697F795489C8710BCC72141FF6FD47684 at ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Cynthia, best practice is to keep in the block and cut as needed. Second is to cut and dry at room temp and not melt, then store. Don't cut, melt and store. The issue is oxidation. Keeping in paraffin prevents oxidation. You'll protect the antigens better if they are fully isolated from air, which paraffin does, but once cut, they are exposed. That happens in the fridge as well and I'm not sure refrigeration helps much. I'm guessing that you don't do a lot of these, so you don't want to cut a lot of slide and have them sitting around for months waiting for use. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cindy Bulmer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 10:43 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC positive controls Hello Histoland, I need some advice, I have a PT block that is positive with Spirochetes. What would be the best way to use this block as a positive control? 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. then put slides in refrigerator for future use. 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides directly in refrigerator for future use. 3) Cut "fresh" every time they order the Ab. Thank you, Cindy Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3aVNXN%2fZov8%3d ------------------------------ Message: 7 Date: Mon, 2 May 2016 19:08:22 +0000 From: "Boyd, Debbie M" To: "Manfre, Philip" Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <7EAFE982E328304DA6CE2B677BB76246AB4B7465 at TN001WEXMBX014.US.chs.net> Content-Type: text/plain; charset="utf-8" I just feel if you didn't care for the subject/blog (doesn't pertain to you, you don't have any exposure to it etc. ) you could have deleted it without being so mean spirited. I have in the past ignored the mean spirited sparring, but this was a bit much. Whatever happened to kindness? Just my two cents.... Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Manfre, Philip via Histonet [histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:38 PM To: Rene J Buesa Cc: 'histonet at lists.utsouthwestern.edu' Subject: [EXTERNAL] Re: [Histonet] No More Blog Posts -- Over and Out! Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3aVNXN%2fZov8%3d _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3aVNXN%2fZov8%3d Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2fwww.merck.com%2fcontact%2fcontacts.html&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=KCoctVheTDqWPFsXArnqT%2b%2fbL9lv0ACuqebB8qSnaU0%3d) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3aVNXN%2fZov8%3d ------------------------------------------------------------------------ -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 8 Date: Mon, 2 May 2016 12:10:44 -0700 From: WILLIAM DESALVO To: "Manfre, Philip" , Rene J Buesa Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: Content-Type: text/plain; charset="utf-8" Your response is WAY out of line. Keep it professional. Sent from my Windows Phone ________________________________ From: Manfre, Philip via Histonet Sent: ?5/?2/?2016 12:08 PM To: Rene J Buesa Cc: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3aVNXN%2fZov8%3d _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3aVNXN%2fZov8%3d Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2fwww.merck.com%2fcontact%2fcontacts.html&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=KCoctVheTDqWPFsXArnqT%2b%2fbL9lv0ACuqebB8qSnaU0%3d) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3aVNXN%2fZov8%3d ------------------------------ Message: 9 Date: Mon, 2 May 2016 14:49:25 -0700 From: Caroline Miller To: Lester Raff MD Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: Content-Type: text/plain; charset=UTF-8 Thank you Lester, I really appreciate your decision, and I look forward to reading your histology-related histonet posts. yours, mills On Mon, May 2, 2016 at 10:39 AM, Lester Raff MD via Histonet < histonet at lists.utsouthwestern.edu> wrote: > To My Lab Colleagues: > > As my intent has never been to sow discontent or rancor, I think it is > for the best if I no longer post links to my blog, lab related or otherwise. > Of course the blogs go on, and if anyone is interested in being added > to my mailing list for future notifications, just drop me a line at > les.raff at post.com The mailing list is never > used for any purpose other than announcing a new blog post. Be sure to > let me know you are from the Histonet list! > > I will continue to participate in any histology/pathologist > discussions here as I have for many years. > > Cheers, > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists. > utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbi > tting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c6 > 64402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3a > VNXN%2fZov8%3d > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 10 Date: Mon, 2 May 2016 15:49:09 -0700 From: WILLIAM DESALVO To: STEVEN PINHEIRO Cc: Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: Content-Type: text/plain; charset="windows-1256" Another personal e-mail from you that is unwanted. I am feeling like I have my own stalker. You are one of the reasons that individuals will not post or comment, personal and ugly. I cannot imagine why your workplace (LUMC.edu) chose you to represent them. I request that you no longer contact me and make personal attacks. I know nothing of you and you certainly do not know anything about me. If you have something to say, post to the listserve, not me. > From: SPINHEIRO at lumc.edu > To: wdesalvo.cac at outlook.com > Subject: RE: [Histonet] No More Blog Posts -- Over and Out! > Date: Mon, 2 May 2016 20:15:38 +0000 > > As is yours. Just can't keep that dictatorial aspect out of your charming consultant two cents worth. > > Steve Pinheiro > > > -----Original Message----- > From: WILLIAM DESALVO via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > Sent: Monday, May 02, 2016 2:11 PM > To: Manfre, Philip; Rene J Buesa > Cc: 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Your response is WAY out of line. Keep it professional. > > Sent from my Windows Phone > ________________________________ > From: Manfre, Philip via > Histonet > Sent: ?5/?2/?2016 12:08 PM > To: Rene J Buesa > Cc: > 'histonet at lists.utsouthwestern.edu' rn.edu> > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! > > -----Original Message----- > From: Rene J Buesa via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > Sent: Monday, May 02, 2016 2:23 PM > To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? > > On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: > > > To My Lab Colleagues: > > As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! > > I will continue to participate in any histology/pathologist discussions here as I have for many years. > > Cheers, > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists. > utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbi > tting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c6 > 64402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3a > VNXN%2fZov8%3d > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists. > utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbi > tting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c6 > 64402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3a > VNXN%2fZov8%3d > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, > Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct > contact information for affiliates is available at > https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2fwww.me > rck.com%2fcontact%2fcontacts.html&data=01%7c01%7cakbitting%40geisinger > .edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b > 910d%7c1&sdata=KCoctVheTDqWPFsXArnqT%2b%2fbL9lv0ACuqebB8qSnaU0%3d) > that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists. > utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbi > tting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c6 > 64402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3a > VNXN%2fZov8%3d _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists. > utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbi > tting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c6 > 64402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3a > VNXN%2fZov8%3d > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 11 Date: Tue, 3 May 2016 13:28:28 +0000 (UTC) From: Rene J Buesa To: Linda Margraf , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Fwd: Message-ID: <1663136023.5351238.1462282108474.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 As I see it, the best solution is "1"Even more: if the piece of tissue is large enough,?cut 1 section and stain ? select at least 2 (+) areas? divide the block into 2 blocks each containing one of those 2 areas?and?by doing so?you would have duplicated the number of possible (+) sections.Ren? On Monday, May 2, 2016 3:09 PM, Linda Margraf via Histonet wrote: > From: Cindy Bulmer > Date: May 2, 2016 at 12:15:22 PM CDT > To: histonet-owner at lists.utsouthwestern.edu > > Hello Histoland, >? > I need some advice, I have a PT block that is positive with Spirochetes. > What would be the best way to use this block as a positive control? >? > 1)? Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. >? ? ? then put slides in refrigerator for future use. > 2)? Cut (serial sections, stain the last slide for bugs) NO oven time? >and put slides ? ? ? directly in refrigerator for future use. > 3)? Cut "fresh" every time they order the Ab. >? > Thank you, > Cindy > Cynthia Bulmer HT(ASCP),QIHC > IHC Supervisor, CTPL > Waco, TX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3aVNXN%2fZov8%3d ------------------------------ Message: 12 Date: Tue, 3 May 2016 16:29:18 +0000 From: Delray Beach Pathology Kari Simeone To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] HHV-8 RTU Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CC918550A at vm-email.leavittmgt.com> Content-Type: text/plain; charset="iso-8859-1" My vendor (Cell Marque/Sigma Aldrich) has a YEAR backorder on this IHC RTU. Does anyone have a vendor they purchase from that I can research? Thanks so much. Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. ------------------------------ Message: 13 Date: Tue, 3 May 2016 16:39:16 +0000 From: "Marcum, Pamela A" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] PAS/Decal Question Message-ID: <826681eae65c40b28de6f620b3207f05 at MAIL13M2N1.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" We are still having issues with our PAS stain on decaled bone marrows. The Pathologists in HemePath are seeing what they refer to as smudginess in cells on some areas of the completed PAS slides. We have looked at everything and cannot find where the issue is coming from at this point. We have done manual staining for PAS, automated on the Leica stainer and on the Dako Artisan. All methods show the same result for some slides. We can go for several days to a week or more with no problem and then suddenly it is back and we have changed nothing in the way we do the processing, embedding, sectioning, deparaffinization and coverslipping. We do as many as 38 bone marrow cores a night or as few as 8 and can find no correlation in the number we have to deal with for a given period. All bone marrows drawn today must be completed by 8AM tomorrow morning. Fixation after pulling the bone marrows is a minimum of 2 hours in AZF with a maximum of 7 hours +/-. Grossed and placed in cassettes for 15 minute rinse in running DI Water Decal currently in the Milestone Decal Unit for 45 minutes in Immunocal at 50C Rinsed in running DI water for 15 minutes Placed in 10% NBF and processed on a 4 hour program with a delay of 4 hours minimum to come off at 4:45AM. If anyone knows of any literature on decal effects on PAS staining in bone marrows please contact me. This has been going on for months and no matter what we do manual staining, Leica adaptation for automated or Dako it is not helping. Dako has been great with sending in technical experts repeatedly and we cannot get this corrected. Thanks, Pam ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3aVNXN%2fZov8%3d ------------------------------ End of Histonet Digest, Vol 150, Issue 2 **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3aVNXN%2fZov8%3d _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c901c6a396ccc4b7dd9a908d3740b93a7%7c37d46c567c664402a16055c2313b910d%7c1&sdata=h7IuxhP%2f%2bMQqlbopCHeX6Ve97tUWI2zS3aVNXN%2fZov8%3d IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. 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From mjdessoye at commonwealthhealth.net Wed May 4 07:20:38 2016 From: mjdessoye at commonwealthhealth.net (Dessoye, Michael) Date: Wed, 4 May 2016 12:20:38 +0000 Subject: [Histonet] Air filter/purifier Message-ID: Hello Histonet, Does anyone have experience with an air filter/purifier for use in the lab? I'm looking mainly for residual toluene fumes. Everything is always below the limit but I have an employee who is unusually sensitive and I'm trying to help out. I found one from Fisher for an astronomical price, and I don't know if there's anything that really makes it different from generic air purifiers you can purchase anywhere. Does anyone use anything that they like? Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From PAMarcum at uams.edu Wed May 4 07:57:52 2016 From: PAMarcum at uams.edu (Marcum, Pamela A) Date: Wed, 4 May 2016 12:57:52 +0000 Subject: [Histonet] Histonet Digest, Vol 150, Issue 2 In-Reply-To: <059D0B145C11034C8C19F60C1B008BA70CDE972D@VHAV06MSGA1.v06.med.va.gov> References: <059D0B145C11034C8C19F60C1B008BA70CDE972D@VHAV06MSGA1.v06.med.va.gov> Message-ID: <0dd9f82d89ca4597a9c35eb0d534bbe5@MAIL13M2N1.ad.uams.edu> We have had troublemakers and rabble rousers on HistoNet before who just are not happy without causing everyone discomfort by their manners/words. The more we feed this and complaint about the worse it will get for all of us. We don't need to answer or get upset just delete and movew on. This is the same as the school yard bully who needs attention anyway they can get it and now they can use the internet and words instead of fists and threats. In this case it is a keyboard and something to make us mad. DELETE and consider the source as my Grandmother would say, "Don't get in the gutter with them". Histology has a great tool and interface for us here so let us focus on the positive more. Pam Marcum -----Original Message----- From: Hudson, Pamela R ASHVAMC via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, May 04, 2016 6:01 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] FW: [EXTERNAL] Re: Histonet Digest, Vol 150, Issue 2 OMG This is terrible. I can't believe I belong to a group of such unprofessional people. Pamela R. Hudson, HT (ASCP) Charles George VA Medical Center Pathology Department 1100 Tunnel Road Asheville, NC 28805 828-298-7911 x5747 Pamela.Hudson at va.gov -----Original Message----- From: T H via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 03, 2016 4:04 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Histonet Digest, Vol 150, Issue 2 Good to know that I am not the only on that think Rene is negative to others. Hey Rene it's not just one person that thinks your a dirt bag. ________________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, May 3, 2016 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 150, Issue 2 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. No More Blog Posts -- Over and Out! (Lester Raff MD) 2. IHC positive controls (Cindy Bulmer) 3. Re: No More Blog Posts -- Over and Out! (Rene J Buesa) 4. Fwd: (Linda Margraf) 5. Re: No More Blog Posts -- Over and Out! (Manfre, Philip) 6. Re: IHC positive controls (Morken, Timothy) 7. Re: No More Blog Posts -- Over and Out! (Boyd, Debbie M) 8. Re: No More Blog Posts -- Over and Out! (WILLIAM DESALVO) 9. Re: No More Blog Posts -- Over and Out! (Caroline Miller) 10. Re: No More Blog Posts -- Over and Out! (WILLIAM DESALVO) 11. Re: Fwd: (Rene J Buesa) 12. HHV-8 RTU (Delray Beach Pathology Kari Simeone) 13. PAS/Decal Question (Marcum, Pamela A) ---------------------------------------------------------------------- Message: 1 Date: Mon, 2 May 2016 17:39:28 +0000 From: Lester Raff MD To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10D5E354 at COLOEXCH01.uropartners.local> Content-Type: text/plain; charset="us-ascii" To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ------------------------------ Message: 2 Date: Mon, 2 May 2016 13:42:36 -0400 From: Cindy Bulmer To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC positive controls Message-ID: <15472908bba-2169-bd52 at webprd-a99.mail.aol.com> Content-Type: text/plain; charset=utf-8 Hello Histoland, I need some advice, I have a PT block that is positive with Spirochetes. What would be the best way to use this block as a positive control? 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. then put slides in refrigerator for future use. 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides directly in refrigerator for future use. 3) Cut "fresh" every time they order the Ab. Thank you, Cindy Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX ------------------------------ Message: 3 Date: Mon, 2 May 2016 18:22:59 +0000 (UTC) From: Rene J Buesa To: Lester Raff MD , "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <1946825210.5059374.1462213379630.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise.? Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 2 May 2016 13:37:35 -0500 From: Linda Margraf To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fwd: Message-ID: <31CF79C7-84C3-45B8-AF7B-20151C22F338 at gmail.com> Content-Type: text/plain; charset=us-ascii > From: Cindy Bulmer > Date: May 2, 2016 at 12:15:22 PM CDT > To: histonet-owner at lists.utsouthwestern.edu > > Hello Histoland, > > I need some advice, I have a PT block that is positive with Spirochetes. > What would be the best way to use this block as a positive control? > > 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. > then put slides in refrigerator for future use. > 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides > directly in refrigerator for future use. > 3) Cut "fresh" every time they order the Ab. > > Thank you, > Cindy > Cynthia Bulmer HT(ASCP),QIHC > IHC Supervisor, CTPL > Waco, TX ------------------------------ Message: 5 Date: Mon, 2 May 2016 14:38:32 -0400 From: "Manfre, Philip" To: Rene J Buesa Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <558A4571351D0C42BD923F403F4198C40111D88D1C5C at USCTMXP51014.merck.com> Content-Type: text/plain; charset="utf-8" Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise.? Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 6 Date: Mon, 2 May 2016 18:47:08 +0000 From: "Morken, Timothy" To: Cindy Bulmer Cc: Histonet Subject: Re: [Histonet] IHC positive controls Message-ID: <761E2B5697F795489C8710BCC72141FF6FD47684 at ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Cynthia, best practice is to keep in the block and cut as needed. Second is to cut and dry at room temp and not melt, then store. Don't cut, melt and store. The issue is oxidation. Keeping in paraffin prevents oxidation. You'll protect the antigens better if they are fully isolated from air, which paraffin does, but once cut, they are exposed. That happens in the fridge as well and I'm not sure refrigeration helps much. I'm guessing that you don't do a lot of these, so you don't want to cut a lot of slide and have them sitting around for months waiting for use. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cindy Bulmer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 10:43 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC positive controls Hello Histoland, I need some advice, I have a PT block that is positive with Spirochetes. What would be the best way to use this block as a positive control? 1) Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. then put slides in refrigerator for future use. 2) Cut (serial sections, stain the last slide for bugs) NO oven time and put slides directly in refrigerator for future use. 3) Cut "fresh" every time they order the Ab. Thank you, Cindy Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 2 May 2016 19:08:22 +0000 From: "Boyd, Debbie M" To: "Manfre, Philip" Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: <7EAFE982E328304DA6CE2B677BB76246AB4B7465 at TN001WEXMBX014.US.chs.net> Content-Type: text/plain; charset="utf-8" I just feel if you didn't care for the subject/blog (doesn't pertain to you, you don't have any exposure to it etc. ) you could have deleted it without being so mean spirited. I have in the past ignored the mean spirited sparring, but this was a bit much. Whatever happened to kindness? Just my two cents.... Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Manfre, Philip via Histonet [histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:38 PM To: Rene J Buesa Cc: 'histonet at lists.utsouthwestern.edu' Subject: [EXTERNAL] Re: [Histonet] No More Blog Posts -- Over and Out! Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 8 Date: Mon, 2 May 2016 12:10:44 -0700 From: WILLIAM DESALVO To: "Manfre, Philip" , Rene J Buesa Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: Content-Type: text/plain; charset="utf-8" Your response is WAY out of line. Keep it professional. Sent from my Windows Phone ________________________________ From: Manfre, Philip via Histonet Sent: ?5/?2/?2016 12:08 PM To: Rene J Buesa Cc: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 02, 2016 2:23 PM To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: To My Lab Colleagues: As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com? The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! I will continue to participate in any histology/pathologist discussions here as I have for many years. Cheers, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 2 May 2016 14:49:25 -0700 From: Caroline Miller To: Lester Raff MD Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: Content-Type: text/plain; charset=UTF-8 Thank you Lester, I really appreciate your decision, and I look forward to reading your histology-related histonet posts. yours, mills On Mon, May 2, 2016 at 10:39 AM, Lester Raff MD via Histonet < histonet at lists.utsouthwestern.edu> wrote: > To My Lab Colleagues: > > As my intent has never been to sow discontent or rancor, I think it is > for the best if I no longer post links to my blog, lab related or otherwise. > Of course the blogs go on, and if anyone is interested in being added > to my mailing list for future notifications, just drop me a line at > les.raff at post.com The mailing list is never > used for any purpose other than announcing a new blog post. Be sure to > let me know you are from the Histonet list! > > I will continue to participate in any histology/pathologist > discussions here as I have for many years. > > Cheers, > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 10 Date: Mon, 2 May 2016 15:49:09 -0700 From: WILLIAM DESALVO To: STEVEN PINHEIRO Cc: Subject: Re: [Histonet] No More Blog Posts -- Over and Out! Message-ID: Content-Type: text/plain; charset="windows-1256" Another personal e-mail from you that is unwanted. I am feeling like I have my own stalker. You are one of the reasons that individuals will not post or comment, personal and ugly. I cannot imagine why your workplace (LUMC.edu) chose you to represent them. I request that you no longer contact me and make personal attacks. I know nothing of you and you certainly do not know anything about me. If you have something to say, post to the listserve, not me. > From: SPINHEIRO at lumc.edu > To: wdesalvo.cac at outlook.com > Subject: RE: [Histonet] No More Blog Posts -- Over and Out! > Date: Mon, 2 May 2016 20:15:38 +0000 > > As is yours. Just can't keep that dictatorial aspect out of your charming consultant two cents worth. > > Steve Pinheiro > > > -----Original Message----- > From: WILLIAM DESALVO via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > Sent: Monday, May 02, 2016 2:11 PM > To: Manfre, Philip; Rene J Buesa > Cc: 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Your response is WAY out of line. Keep it professional. > > Sent from my Windows Phone > ________________________________ > From: Manfre, Philip via > Histonet > Sent: ?5/?2/?2016 12:08 PM > To: Rene J Buesa > Cc: > 'histonet at lists.utsouthwestern.edu' rn.edu> > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Delete button broken, huh. I suppose it was necessary to attack a colleague to feel puffed up about yourself. Bully for you! > > -----Original Message----- > From: Rene J Buesa via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > Sent: Monday, May 02, 2016 2:23 PM > To: Lester Raff MD; 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] No More Blog Posts -- Over and Out! > > Thank you VERY MUCH!!!!!!!!!!!!!!!!!!!!!!!!!!!!!Ren? > > On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet wrote: > > > To My Lab Colleagues: > > As my intent has never been to sow discontent or rancor, I think it is for the best if I no longer post links to my blog, lab related or otherwise. Of course the blogs go on, and if anyone is interested in being added to my mailing list for future notifications, just drop me a line at les.raff at post.com The mailing list is never used for any purpose other than announcing a new blog post. Be sure to let me know you are from the Histonet list! > > I will continue to participate in any histology/pathologist discussions here as I have for many years. > > Cheers, > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, > Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct > contact information for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 11 Date: Tue, 3 May 2016 13:28:28 +0000 (UTC) From: Rene J Buesa To: Linda Margraf , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Fwd: Message-ID: <1663136023.5351238.1462282108474.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 As I see it, the best solution is "1"Even more: if the piece of tissue is large enough,?cut 1 section and stain ? select at least 2 (+) areas? divide the block into 2 blocks each containing one of those 2 areas?and?by doing so?you would have duplicated the number of possible (+) sections.Ren? On Monday, May 2, 2016 3:09 PM, Linda Margraf via Histonet wrote: > From: Cindy Bulmer > Date: May 2, 2016 at 12:15:22 PM CDT > To: histonet-owner at lists.utsouthwestern.edu > > Hello Histoland, >? > I need some advice, I have a PT block that is positive with Spirochetes. > What would be the best way to use this block as a positive control? >? > 1)? Cut (serial sections, stain the last slide for bugs) and oven time (60) for 1 hr. >? ? ? then put slides in refrigerator for future use. > 2)? Cut (serial sections, stain the last slide for bugs) NO oven time? >and put slides ? ? ? directly in refrigerator for future use. > 3)? Cut "fresh" every time they order the Ab. >? > Thank you, > Cindy > Cynthia Bulmer HT(ASCP),QIHC > IHC Supervisor, CTPL > Waco, TX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 3 May 2016 16:29:18 +0000 From: Delray Beach Pathology Kari Simeone To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] HHV-8 RTU Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CC918550A at vm-email.leavittmgt.com> Content-Type: text/plain; charset="iso-8859-1" My vendor (Cell Marque/Sigma Aldrich) has a YEAR backorder on this IHC RTU. Does anyone have a vendor they purchase from that I can research? Thanks so much. Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. ------------------------------ Message: 13 Date: Tue, 3 May 2016 16:39:16 +0000 From: "Marcum, Pamela A" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] PAS/Decal Question Message-ID: <826681eae65c40b28de6f620b3207f05 at MAIL13M2N1.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" We are still having issues with our PAS stain on decaled bone marrows. The Pathologists in HemePath are seeing what they refer to as smudginess in cells on some areas of the completed PAS slides. We have looked at everything and cannot find where the issue is coming from at this point. We have done manual staining for PAS, automated on the Leica stainer and on the Dako Artisan. All methods show the same result for some slides. We can go for several days to a week or more with no problem and then suddenly it is back and we have changed nothing in the way we do the processing, embedding, sectioning, deparaffinization and coverslipping. We do as many as 38 bone marrow cores a night or as few as 8 and can find no correlation in the number we have to deal with for a given period. All bone marrows drawn today must be completed by 8AM tomorrow morning. Fixation after pulling the bone marrows is a minimum of 2 hours in AZF with a maximum of 7 hours +/-. Grossed and placed in cassettes for 15 minute rinse in running DI Water Decal currently in the Milestone Decal Unit for 45 minutes in Immunocal at 50C Rinsed in running DI water for 15 minutes Placed in 10% NBF and processed on a 4 hour program with a delay of 4 hours minimum to come off at 4:45AM. If anyone knows of any literature on decal effects on PAS staining in bone marrows please contact me. This has been going on for months and no matter what we do manual staining, Leica adaptation for automated or Dako it is not helping. Dako has been great with sending in technical experts repeatedly and we cannot get this corrected. Thanks, Pam ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 150, Issue 2 **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas at biopath.org Wed May 4 08:31:10 2016 From: plucas at biopath.org (Paula) Date: Wed, 4 May 2016 06:31:10 -0700 Subject: [Histonet] Wrist pain from embedding Message-ID: <001f01d1a609$39bb2ea0$ad318be0$@biopath.org> Hello, Does anyone have a remedy for wrist pain while embedding or can offer a suggestion on what to wear during the embedding process for one of my technicians to wear? She'll be off the bench for a little while but I was wondering if there is something that would help her. Thank you in advance, Paula From boneslides at aol.com Wed May 4 08:39:10 2016 From: boneslides at aol.com (boneslides at aol.com) Date: Wed, 4 May 2016 09:39:10 -0400 Subject: [Histonet] Fw:0 762q62i7d Message-ID: <1547bfe6796-5ba0-13330@webprd-a31.mail.aol.com> http://occmcompany.com/mind.php From: boneslides Code: hrnn5p6hy1a87m1cwpm Time: 5/4/2016 2:39:10 PM From relia1 at earthlink.net Wed May 4 10:30:58 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 4 May 2016 11:30:58 -0400 Subject: [Histonet] RELIA HOT Histology Job Alert 5-4-2016 Exciting opportunities in Austin, TX Message-ID: <00ed01d1a619$f5753ef0$e05fbcd0$@earthlink.net> Hi Histopeeps! Just a quick post to tell you about some exciting new opportunities in Hip, HOT exciting Austin, TX RELIA Solutions has been engaged EXCLUSIVELY by a growing lab located in Austin, TX to recruit experienced certified histotechs. You will be responsible for performing routine histology, cutting, embedding and staining. ASCP HT/HTL. and 1-2 years of histology experience is required. Strong Derm, High volume and/or Special Stains experience preferred. My client offers excellent compensation a generous shift differential, great benefits, relocation assistance and a great team to work with. My client is eager to speak with you and ready to hire!! For more information please contact Pam Barker at relia1 at earthlink.net or toll free at 866-607-3542 or on my cell at 407-353-5070. RELIA Solutions is the nation's ONLY recruiting firm specializing in the nationwide permanent placement of histology professionals. To sign up for our free histology careers bulletin please send an e-mail to relia1 at earthlink.net and include subscribe in the subject line. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From scrochiere at nedlc.com Wed May 4 10:33:31 2016 From: scrochiere at nedlc.com (Steven Crochiere) Date: Wed, 4 May 2016 11:33:31 -0400 Subject: [Histonet] Per diem histo position Message-ID: <000501d1a61a$50da9d80$f28fd880$@nedlc.com> I currently am in need of a perdiem HT to cover for vacations and absences in my lab. Please contact: Steve Crochiere New England Dermatology 3455 main st Springfield MA 01107 scrochiere at nedlc.com Thanks From murphyv at karmanos.org Wed May 4 10:51:38 2016 From: murphyv at karmanos.org (Murphy, Valerie) Date: Wed, 4 May 2016 15:51:38 +0000 Subject: [Histonet] Automated IHC instrument Message-ID: Hello Histonetters, Our tissue core is interested in purchasing an IHC instrument. It would be used for the more routine assays such as ER/PR, Her2, Ki67 etc. Can anyone make a recommendation? Leica, Ventana, Dako? Thank you, Valerie Ratliff B.Sc HTL(ASCP) Research Assistant Department of Oncology Karmanos Cancer Institute 4100 John R Detroit, MI 48201 Telephone: (313) 576 8282 Fax: (313) 576 8306 E-mail: murphyv at karmanos.org Better treatments. Better outcomes. ----------- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. From rjbuesa at yahoo.com Wed May 4 11:24:18 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 4 May 2016 16:24:18 +0000 (UTC) Subject: [Histonet] Automated IHC instrument In-Reply-To: References: Message-ID: <913267069.6014768.1462379058943.JavaMail.yahoo@mail.yahoo.com> I tested those you mention and leased/used the one from DAKO and "never looked back".Ren? On Wednesday, May 4, 2016 12:13 PM, "Murphy, Valerie via Histonet" wrote: Hello Histonetters, Our tissue core is interested in purchasing an IHC instrument. It would? be used for the more routine assays such as ER/PR, Her2, Ki67 etc. Can anyone make a recommendation? Leica, Ventana, Dako? Thank you, Valerie Ratliff? B.Sc HTL(ASCP) Research Assistant Department of Oncology Karmanos Cancer Institute 4100 John R Detroit, MI 48201 Telephone: (313) 576 8282 Fax: (313) 576 8306 E-mail: murphyv at karmanos.org Better treatments. Better outcomes. ----------- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mdmanzer at ucdavis.edu Wed May 4 11:30:46 2016 From: mdmanzer at ucdavis.edu (Michael David Manzer) Date: Wed, 4 May 2016 16:30:46 +0000 Subject: [Histonet] Histology Supervisor Needed Message-ID: Apply at UC-Davis VMTH: Requistion number 03016417 https://www.employment.ucdavis.edu/applicants/jsp/shared/position/JobDetails_css.jsp?postingId=309995 Mike Manzer, HTL UC Davis, VMTH Anatomic Pathology - IHC Lab mdmanzer at ucdavis.edu (530) 752-3901 From philip_manfre at merck.com Wed May 4 12:09:54 2016 From: philip_manfre at merck.com (Manfre, Philip) Date: Wed, 4 May 2016 13:09:54 -0400 Subject: [Histonet] Automated IHC instrument In-Reply-To: References: Message-ID: <558A4571351D0C42BD923F403F4198C40111D8978164@USCTMXP51014.merck.com> Valerie, We have 4 DAKOs. They are work-horses and are a very "open" platform. You can use all of your own reagents, nothing proprietary if you choose. This makes them good for research and optimizing your own protocols. They are also very consistent and reliable for conducting the same protocol repeatedly. Much cheaper to own and operate than a Ventana. This is not a slam on Ventana. Ventana strainers are quite amazing but way more expensive than a DAKO. The Ventana is partially open, or not open, depending on how you look at it. You can force it to use some of your own reagents but you always rely somewhat on Ventana reagents. These stainers are mostly designed to run the same set of protocols over and over again, consistently (e.g. hospital). They are also useful when used by technicians who want to "set it and forget it". If someone doesn't really know IHC, and the troubleshooting involved sometimes, then this is the unit for you if you can afford it. There is also the high pressure sales team that accompanies any interest in Ventana units. Don't underestimate their tenacity, especially if you agree to have a trial machine placed in your lab for testing. They will not want to remove it - an assumed sale, if you will. I am not on the payroll for anyone but Merck, lest anyone think I am trying to influence a sale one way or another. These are my personal experiences and opinions that I hope you will find useful. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre at merck.com -----Original Message----- From: Murphy, Valerie via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, May 04, 2016 11:52 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Automated IHC instrument Hello Histonetters, Our tissue core is interested in purchasing an IHC instrument. It would be used for the more routine assays such as ER/PR, Her2, Ki67 etc. Can anyone make a recommendation? Leica, Ventana, Dako? Thank you, Valerie Ratliff B.Sc HTL(ASCP) Research Assistant Department of Oncology Karmanos Cancer Institute 4100 John R Detroit, MI 48201 Telephone: (313) 576 8282 Fax: (313) 576 8306 E-mail: murphyv at karmanos.org Better treatments. Better outcomes. ----------- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From tbraud at holyredeemer.com Wed May 4 12:19:59 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 4 May 2016 17:19:59 +0000 Subject: [Histonet] Wrist Pain Message-ID: <48E053DDF6CE074DB6A7414BA05403F807195E@HRHEX02-HOS.holyredeemer.local> This is an repetitive stress injury and is not to be trifled with. I know that I have a tech that occasionally uses a wrist brace to embed, but also to sleep with at night. I strongly recommend using ergonomic friendly embedding forceps available from selected Histology supply companies. Also, for the technician to be seen by your employers occupational health provider for appropriate documentation and follow-up. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 From BradleyJay at UH.ORG Wed May 4 12:35:19 2016 From: BradleyJay at UH.ORG (Bradley Jay) Date: Wed, 4 May 2016 13:35:19 -0400 Subject: [Histonet] qihc exam Message-ID: From Jennifer.Johnson at genzyme.com Wed May 4 14:10:11 2016 From: Jennifer.Johnson at genzyme.com (Jennifer.Johnson at genzyme.com) Date: Wed, 4 May 2016 19:10:11 +0000 Subject: [Histonet] replacing a stainer and a coverslipper question Message-ID: <0C0766AB928A0E4DB4760C0DB41F91B9D1CC6E60@XSPW10A507T.pharma.aventis.com> Dear Histonetters, We are going to replace our automated stainer (LeicaXL) and coverslipper within the next few months. I would really appreciate any suggestions that people may have about which stainer/coverslipper units they use/used and are happy with. I am also curious about tape vs glass coverslippers. We have always had glass here, but I know that there are people who love their tape units. I would be willing to look at a tape unit, but I am concerned about long term storage issues. I have seen a few postings lately about the tape coming off of the slides and that scares me! I would appreciate any advice on what brands work well and which ones to avoid, if you are willing to share... If you have any insight that you would like to share, you can post it or e-mail me directly, especially if it is about which units/tapes to avoid. I don't want to vendor bash, so please feel free to email me directly at jennifer.johnson at genzyme.com Thanks! Jenn Jennifer Johnson, B.S., HTL (ASCP) Scientist Department of Pathology TEL.: 508-271-3610 - Fax.: 508-872-9080 5 The Mountain Road, Framingham, MA 01701-9322 From jclark at pcnm.com Wed May 4 15:02:48 2016 From: jclark at pcnm.com (Joanne Clark) Date: Wed, 4 May 2016 20:02:48 +0000 Subject: [Histonet] PAS Stain Message-ID: <7A7BDD92B984E847A7E71BC9C00A66D312722541@S11MAILD034N2.sh11.lan> Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS diastase method. We have been digesting the tissue in 0.5% diastase of malt in a 60 degree oven for 30 minutes, but do not see the glycogen being digested out. I have tried alpha amylase and beta amylase also without any luck. Does anyone have any suggestions to get the digestion to work Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From jaylundgren at gmail.com Wed May 4 16:21:36 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 4 May 2016 14:21:36 -0700 Subject: [Histonet] Wrist Pain In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F807195E@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F807195E@HRHEX02-HOS.holyredeemer.local> Message-ID: I worked at a lab in Oregon that only let a tech embed for an hour at a time, before having someone else take over. They were very concerned about carpal tunnel prevention, obviously. They just did it informally, no schedule or anything. After an hour, you would stand up and say, "Who wants to embed?", and one of the cutters, who was at a stopping point, would take over. The ergonomic forceps from Statlab are awesome. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Wed, May 4, 2016 at 10:19 AM, Terri Braud via Histonet < histonet at lists.utsouthwestern.edu> wrote: > This is an repetitive stress injury and is not to be trifled with. I know > that I have a tech that occasionally uses a wrist brace to embed, but also > to sleep with at night. > I strongly recommend using ergonomic friendly embedding forceps available > from selected Histology supply companies. Also, for the technician to be > seen by your employers occupational health provider for appropriate > documentation and follow-up. > Sincerely, Terri > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pdefazio802 at gmail.com Wed May 4 19:32:57 2016 From: pdefazio802 at gmail.com (Pam DeFazio) Date: Wed, 4 May 2016 20:32:57 -0400 Subject: [Histonet] Histotech's working on Saturday? Message-ID: Does your hospital require working Saturday's? Particularly interested in hospitals in the southeast area. From gu.lang at gmx.at Thu May 5 01:48:46 2016 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 5 May 2016 08:48:46 +0200 Subject: [Histonet] PAS Stain In-Reply-To: <7A7BDD92B984E847A7E71BC9C00A66D312722541@S11MAILD034N2.sh11.lan> References: <7A7BDD92B984E847A7E71BC9C00A66D312722541@S11MAILD034N2.sh11.lan> Message-ID: <002101d1a69a$2e0b9360$8a22ba20$@gmx.at> As far as I remember the incubation temperature is at roomtemperature. 60?C would rather denature the native enzyme than increase activity. Look at the optimal working temp of the reagens you have bought. regards Gudrun -----Urspr?ngliche Nachricht----- Von: Joanne Clark via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 04. Mai 2016 22:03 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] PAS Stain Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS diastase method. We have been digesting the tissue in 0.5% diastase of malt in a 60 degree oven for 30 minutes, but do not see the glycogen being digested out. I have tried alpha amylase and beta amylase also without any luck. Does anyone have any suggestions to get the digestion to work Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang at gmx.at Thu May 5 02:19:15 2016 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 5 May 2016 09:19:15 +0200 Subject: [Histonet] PAS/Decal Question In-Reply-To: <826681eae65c40b28de6f620b3207f05@MAIL13M2N1.ad.uams.edu> References: <826681eae65c40b28de6f620b3207f05@MAIL13M2N1.ad.uams.edu> Message-ID: <002501d1a69e$6f8bede0$4ea3c9a0$@gmx.at> Hi Pam, my personal opinion is, that 2 hours fixation is too short for sufficient tissue-protection before acid decalcification. Formic acid at 50?C must have an impact on glycoproteins. Wether it is a kind of solving the "sugars" or beginning oxidation of the OH-groups (like periodic acid does in the PAS-reaction). In our lab we do also acid decal with formic acid for at least 6 hours at RT, after one day in NBF. Our processing protocol is the routine-protocol over night. How thick are your BMT, also 3-4 mm? In my opinion 4 hours are a challenge. Are the other stainings of the BMT optimal or show sometimes similar outcome? "Smudginess" reminds me of insufficient infiltration. I also see that our PAS is not as bright as in the other specimens without decal. Sometimes it gives more the impression of a diastase-PAS. Gudrun -----Urspr?ngliche Nachricht----- Von: Marcum, Pamela A via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Dienstag, 03. Mai 2016 18:39 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] PAS/Decal Question We are still having issues with our PAS stain on decaled bone marrows. The Pathologists in HemePath are seeing what they refer to as smudginess in cells on some areas of the completed PAS slides. We have looked at everything and cannot find where the issue is coming from at this point. We have done manual staining for PAS, automated on the Leica stainer and on the Dako Artisan. All methods show the same result for some slides. We can go for several days to a week or more with no problem and then suddenly it is back and we have changed nothing in the way we do the processing, embedding, sectioning, deparaffinization and coverslipping. We do as many as 38 bone marrow cores a night or as few as 8 and can find no correlation in the number we have to deal with for a given period. All bone marrows drawn today must be completed by 8AM tomorrow morning. Fixation after pulling the bone marrows is a minimum of 2 hours in AZF with a maximum of 7 hours +/-. Grossed and placed in cassettes for 15 minute rinse in running DI Water Decal currently in the Milestone Decal Unit for 45 minutes in Immunocal at 50C Rinsed in running DI water for 15 minutes Placed in 10% NBF and processed on a 4 hour program with a delay of 4 hours minimum to come off at 4:45AM. If anyone knows of any literature on decal effects on PAS staining in bone marrows please contact me. This has been going on for months and no matter what we do manual staining, Leica adaptation for automated or Dako it is not helping. Dako has been great with sending in technical experts repeatedly and we cannot get this corrected. Thanks, Pam ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From philip_manfre at merck.com Thu May 5 06:49:06 2016 From: philip_manfre at merck.com (Manfre, Philip) Date: Thu, 5 May 2016 07:49:06 -0400 Subject: [Histonet] PAS Stain In-Reply-To: <7A7BDD92B984E847A7E71BC9C00A66D312722541@S11MAILD034N2.sh11.lan> References: <7A7BDD92B984E847A7E71BC9C00A66D312722541@S11MAILD034N2.sh11.lan> Message-ID: <558A4571351D0C42BD923F403F4198C40111D8A0D868@USCTMXP51014.merck.com> We switched to alpha amylase since the diastase we were ordering was not working. We use a 2% solution of alpha amylase for 40 minutes at room temperature and it has been working fine. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre at merck.com -----Original Message----- From: Joanne Clark via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, May 04, 2016 4:03 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] PAS Stain Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS diastase method. We have been digesting the tissue in 0.5% diastase of malt in a 60 degree oven for 30 minutes, but do not see the glycogen being digested out. I have tried alpha amylase and beta amylase also without any luck. Does anyone have any suggestions to get the digestion to work Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From rjbuesa at yahoo.com Thu May 5 07:19:29 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 5 May 2016 12:19:29 +0000 (UTC) Subject: [Histonet] replacing a stainer and a coverslipper question In-Reply-To: <0C0766AB928A0E4DB4760C0DB41F91B9D1CC6E60@XSPW10A507T.pharma.aventis.com> References: <0C0766AB928A0E4DB4760C0DB41F91B9D1CC6E60@XSPW10A507T.pharma.aventis.com> Message-ID: <1075692632.6542140.1462450769924.JavaMail.yahoo@mail.yahoo.com> Productivity and quality sake, Sakura film coverslipper has no match. If you use Sakura tape and the xylene dispenser is properly calibrated, storage is not an issue.Sakura stainer was also what I used at my lab and I highly recommend both.Ren? On Wednesday, May 4, 2016 3:19 PM, Jenn via Histonet wrote: Dear Histonetters, We are going to replace our automated stainer (LeicaXL) and coverslipper within the next few months.? I would really appreciate any suggestions that people may have about which stainer/coverslipper units they use/used and are happy with. I am also curious about tape vs glass coverslippers.? We have always had glass here, but I know that there are people who love their tape units.? I would be willing to look at a tape unit, but I am concerned about long term storage issues.? I have seen a few postings lately about the tape coming off of the slides and that scares me!? I would appreciate any advice on what brands work well and which ones to avoid, if you are willing to share... If you have any insight that you would like to share, you can post it or e-mail me directly, especially if it is about which units/tapes to avoid.? I don't want to vendor bash, so please feel free to email me directly? at jennifer.johnson at genzyme.com Thanks! Jenn Jennifer Johnson, B.S., HTL (ASCP) Scientist Department of Pathology TEL.: 508-271-3610? -? Fax.: 508-872-9080 5 The Mountain Road, Framingham, MA 01701-9322 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From james.fortune at unitypoint.org Thu May 5 08:24:58 2016 From: james.fortune at unitypoint.org (Fortune, James A.) Date: Thu, 5 May 2016 13:24:58 +0000 Subject: [Histonet] pas diastase Message-ID: We use a .5% to 1% solution of diastase for our fungal pas (so the docs can see the fungus better) and leave it in for 25-30 min at room temp and have had great results. We use American mastertech brand of diastase. Andy Fortune This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. SSSS 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From therndon at gmi-inc.com Thu May 5 09:00:19 2016 From: therndon at gmi-inc.com (Travis Herndon) Date: Thu, 5 May 2016 14:00:19 +0000 Subject: [Histonet] Open invite Message-ID: <94E4C52BB571D647BEA65EBB03750E022E066909@GMI-2011.GMI.local> Hello Histonet users, I would like to welcome anyone who is in the Minneapolis MN area to visit our facilities in Ramsey MN. Even though I have seen some back and forth tension on here. Overall it seems like a great group of people that are passionate about their work. This would not be a pressure sales thing (we are not going to trap you in a room and throw why we are great at you). Just a chance to see who GMI is as a company and what we do. If you're in the area let me know. Kind regards, Travis Herndon Technical Sales Consultant GMI therndon at gmi-inc.com | www.gmi-inc.com Advancing Science with Affordable Solutions ISO 9001:2008 Certified T: 763 712-8717 ext. 6826 | F: 763 712-8724 Cell:612-803-1820 www.linkedin.com/in/travisherndon From sandra.cheasty at wisc.edu Thu May 5 09:36:44 2016 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Thu, 05 May 2016 14:36:44 +0000 Subject: [Histonet] Animals Need Histologist Too! Message-ID: Hello All! We are looking for a dedicated Histologist, with at least 1 year of experience in a full service histology lab, to join our Veterinary Pathology team. Our Histology lab serves the Veterinary Medical Teaching Hospital in Madison, Wisconsin, as well as many research needs at the School of Veterinary Medicine. We are a full service lab providing processing, embedding, microtomy, histochemical and immunohistochemical staining. Full-time, Days, Monday through Friday You can reply to me directly, or follow the link below to apply. http://www.ohr.wisc.edu/Weblisting/External/PDSummaryApply.aspx?vacid=97933&title=35062 Thank you! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From criley at dpspa.com Thu May 5 10:57:51 2016 From: criley at dpspa.com (Charles Riley) Date: Thu, 5 May 2016 11:57:51 -0400 Subject: [Histonet] Stat Lab IHC's Message-ID: Does anyone have experience using any of STATLAb's new antibodies or their new IHC platform? Can you please tell me what you think the advantages and disadvantages are to their products? -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From koellingr at comcast.net Thu May 5 13:05:53 2016 From: koellingr at comcast.net (koellingr at comcast.net) Date: Thu, 5 May 2016 18:05:53 +0000 (UTC) Subject: [Histonet] PAS Stain In-Reply-To: <7A7BDD92B984E847A7E71BC9C00A66D312722541@S11MAILD034N2.sh11.lan> References: <7A7BDD92B984E847A7E71BC9C00A66D312722541@S11MAILD034N2.sh11.lan> Message-ID: <964815213.24575419.1462471553090.JavaMail.zimbra@comcast.net> Hello ? Agree with comments about temperature of 60 degrees killing an enzyme.? If you plot enzyme activity on "y"??axis and temperature on "x" axis it is not a straight line.? Every enzyme has an optimal temperature and can function slowly at non-optimal or optimally at correct temperature.? Diastase/amylase from your body work at 37 degrees, less so or dead at RT or 60 degrees.? Nuking a mixture to 37 degrees might nuke protein.? Nuke buffer to 37, verify temp and add the enzyme. ? pH-if you plot enzyme activity on "y" axis and pH on x-axis, it is not a straight line.? There is an optimal pH for each enzyme.? The enzyme might work fine at different pH; just not nearly as well. ? I know some still use 0.5% or 1.0% in di water.? I'd advise not to.? Use a buffer at a given pH.? "pH?ng" di or distilled water with ordinary lab electrodes is an act of futility.? From a standard lab electrode point of view, there is NO pH to read.? Not enough ions present so meter might say anything.? If anything, it will pick up CO2 from air which becomes carbonic acid and meter will flail wildly in acidic region.? But that is not a stable buffer.? di water might work.? Just that sometimes, it?might?not.? Why chance it???di water?has unstable (no reliably readable) pH. ? Ray Koelling, golfing in Spokane WA and enjoying the low humidity ----- Original Message ----- From: "Joanne Clark via Histonet" To: histonet at lists.utsouthwestern.edu Sent: Wednesday, May 4, 2016 1:02:48 PM Subject: [Histonet] PAS Stain Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS diastase method. ?We have been digesting the tissue in 0.5% diastase of malt in a 60 degree oven for 30 minutes, but do not see the glycogen being digested out. ?I have tried alpha amylase and beta amylase also without any luck. ?Does anyone have any suggestions to get the digestion to work Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. ? (575) 622-5600 C. ? (575) 317-6403 F. ? (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Thu May 5 13:10:40 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 5 May 2016 14:10:40 -0400 Subject: [Histonet] PAS Stain Message-ID: Amylase (diastase) for the PAS stain queries: Whatever happened to spitting on the slide (30 min at room temperature)? John Kiernan advises "thinking of lemons and drooling into a small beaker" though I'd advise chewing on a rubber band for a few seconds. He notes that alpha amylase is preferred. I'd go with the cheapest one in the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma offers a heat-stable alpha amylase. Bob Richmond Samurai Pathologist Maryville TN From JMacDonald at mtsac.edu Thu May 5 13:15:00 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Thu, 5 May 2016 11:15:00 -0700 Subject: [Histonet] PAS Stain In-Reply-To: <7A7BDD92B984E847A7E71BC9C00A66D312722541@S11MAILD034N2.sh11.lan> References: <7A7BDD92B984E847A7E71BC9C00A66D312722541@S11MAILD034N2.sh11.lan> Message-ID: We switched form malt diastase to alpha amylase and have seen a significant improvement in digestion, but as others have said enzyme activity will be destroyed at 60 degrees C. We put ours into a 37 degree C water bath. Enzymes work optimally at 37C, body temperature. As the temperature rises above 37C you will see a decrease in enzyme activity and then none at all. Jennifer From: Joanne Clark via Histonet To: "histonet at lists.utsouthwestern.edu" Date: 05/04/2016 01:04 PM Subject: [Histonet] PAS Stain Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS diastase method. We have been digesting the tissue in 0.5% diastase of malt in a 60 degree oven for 30 minutes, but do not see the glycogen being digested out. I have tried alpha amylase and beta amylase also without any luck. Does anyone have any suggestions to get the digestion to work Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr at comcast.net Thu May 5 13:27:08 2016 From: koellingr at comcast.net (koellingr at comcast.net) Date: Thu, 5 May 2016 18:27:08 +0000 (UTC) Subject: [Histonet] PAS Stain In-Reply-To: References: Message-ID: <1015261985.24592150.1462472828158.JavaMail.zimbra@comcast.net> I love having the Samuri Pathologist on this forum for wisdom and real-laboratory life knowledge.? And yes, I have in the past spit on slide ON OCCASSION when faced with a dire necessity.? Although I know there are those who would wretch about this; it remains a fact of viable laboratory life for some. ? My problem now is that in this era of (MUCH TOO MUCH) regulation, how do you "test lots" or control from "lot-to-lot variation" in this SOP?? When Jane or Joe do this routinely and then goes on vacation, what about Sally or Jim spit?? There is a variation in copy number of the AMY1 gene (amylase) and resulting difference in amylase protein concentration amongst individuals. ? Why not just standardize it from the start, reagent, pH, temperature and it really cannot fail. ? Spokane Ray ----- Original Message ----- From: "Bob Richmond via Histonet" To: "Histonet at lists.utsouthwestern.edu" Sent: Thursday, May 5, 2016 11:10:40 AM Subject: Re: [Histonet] PAS Stain Amylase (diastase) for the PAS stain queries: Whatever happened to spitting on the slide (30 min at room temperature)? John Kiernan advises "thinking of lemons and drooling into a small beaker" though I'd advise chewing on a rubber band for a few seconds. He notes that alpha amylase is preferred. I'd go with the cheapest one in the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma offers a heat-stable alpha amylase. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Thu May 5 13:35:42 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 5 May 2016 14:35:42 -0400 Subject: [Histonet] PAS Stain In-Reply-To: <1015261985.24592150.1462472828158.JavaMail.zimbra@comcast.net> References: <1015261985.24592150.1462472828158.JavaMail.zimbra@comcast.net> Message-ID: Spokane Ray points out something I've wondered about for years - can just anybody spit on the slide and remove the glycogen? I've never heard of any variation, but the number of people I've asked is very limited. This reference: http://www.ncbi.nlm.nih.gov/gene/276 certainly suggests that different people have different salivary alpha amylase activity. Bob Richmond On Thu, May 5, 2016 at 2:27 PM, wrote: > I love having the Samuri Pathologist on this forum for wisdom and > real-laboratory life knowledge. And yes, I have in the past spit on slide > ON OCCASSION when faced with a dire necessity. Although I know there are > those who would wretch about this; it remains a fact of viable laboratory > life for some. > > My problem now is that in this era of (MUCH TOO MUCH) regulation, how do > you "test lots" or control from "lot-to-lot variation" in this SOP? When > Jane or Joe do this routinely and then goes on vacation, what about Sally > or Jim spit? There is a variation in copy number of the AMY1 gene > (amylase) and resulting difference in amylase protein concentration amongst > individuals. > > Why not just standardize it from the start, reagent, pH, temperature and > it really cannot fail. > > Spokane Ray > > ------------------------------ > *From: *"Bob Richmond via Histonet" > *To: *"Histonet at lists.utsouthwestern.edu" < > histonet at lists.utsouthwestern.edu> > *Sent: *Thursday, May 5, 2016 11:10:40 AM > *Subject: *Re: [Histonet] PAS Stain > > > Amylase (diastase) for the PAS stain queries: > > Whatever happened to spitting on the slide (30 min at room temperature)? > John Kiernan advises "thinking of lemons and drooling into a small beaker" > though I'd advise chewing on a rubber band for a few seconds. > > He notes that alpha amylase is preferred. I'd go with the cheapest one in > the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma > offers a heat-stable alpha amylase. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jvickroy at SpringfieldClinic.com Thu May 5 13:43:01 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Thu, 5 May 2016 18:43:01 +0000 Subject: [Histonet] Grossing of oriented skin biopsies and small lymph nodes Message-ID: <9B1A1501A800064397369BD8072E6BCA06552C12@E2K10DB.springfieldclinic.com> Currently our non-pathologists do not gross oriented skin excisions or small lymph nodes. I do understand that CAP requires a list of tissues that can be handled by non-pathologists but am wondering how others are handling these specimens. I know there are labs, for example some large dermatopathology labs that are having the oriented skin biopsies grossed by techs. Of course a accepted protocol has to be listed for the dissection. Let me know how your institution are handling these specimens. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From koellingr at comcast.net Thu May 5 13:48:15 2016 From: koellingr at comcast.net (koellingr at comcast.net) Date: Thu, 5 May 2016 18:48:15 +0000 (UTC) Subject: [Histonet] PAS Stain In-Reply-To: References: <1015261985.24592150.1462472828158.JavaMail.zimbra@comcast.net> Message-ID: <922426283.24607846.1462474095938.JavaMail.zimbra@comcast.net> An excellent point.? For anyone wanting to investigate-simply do a PubMed search on variation of AMY1 gene.? Sorry; I?guess I should say this is, strictly speaking, non-histology related topic and I don't want to get into trouble as some before me.? Tons of research about this linking back (in theory) to positive selection in hunter-gatherers versus agricultural ancestors, race, obesity, phenotypic and dietary differences as to why maybe there can be big differences. Spokane Ray ----- Original Message ----- From: "Bob Richmond" To: koellingr at comcast.net Cc: "Histonet at lists.utsouthwestern.edu" Sent: Thursday, May 5, 2016 11:35:42 AM Subject: Re: [Histonet] PAS Stain Spokane Ray points out something I've wondered about for years - can just anybody spit on the slide and remove the glycogen? I've never heard of any variation, but the number of people I've asked is very limited. This reference: http://www.ncbi.nlm.nih.gov/gene/276 certainly suggests that different people have different salivary alpha amylase activity. Bob Richmond On Thu, May 5, 2016 at 2:27 PM, < koellingr at comcast.net > wrote: I love having the Samuri Pathologist on this forum for wisdom and real-laboratory life knowledge.? And yes, I have in the past spit on slide ON OCCASSION when faced with a dire necessity.? Although I know there are those who would wretch about this; it remains a fact of viable laboratory life for some. ? My problem now is that in this era of (MUCH TOO MUCH) regulation, how do you "test lots" or control from "lot-to-lot variation" in this SOP?? When Jane or Joe do this routinely and then goes on vacation, what about Sally or Jim spit?? There is a variation in copy number of the AMY1 gene (amylase) and resulting difference in amylase protein concentration amongst individuals. ? Why not just standardize it from the start, reagent, pH, temperature and it really cannot fail. ? Spokane Ray From: "Bob Richmond via Histonet" < histonet at lists.utsouthwestern.edu > To: " Histonet at lists.utsouthwestern.edu " < histonet at lists.utsouthwestern.edu > Sent: Thursday, May 5, 2016 11:10:40 AM Subject: Re: [Histonet] PAS Stain Amylase (diastase) for the PAS stain queries: Whatever happened to spitting on the slide (30 min at room temperature)? John Kiernan advises "thinking of lemons and drooling into a small beaker" though I'd advise chewing on a rubber band for a few seconds. He notes that alpha amylase is preferred. I'd go with the cheapest one in the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma offers a heat-stable alpha amylase. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwerdler at gmail.com Thu May 5 14:08:44 2016 From: mwerdler at gmail.com (Mca Werdler) Date: Thu, 5 May 2016 14:08:44 -0500 Subject: [Histonet] picric acid Message-ID: Dear histonetters, Since a few months, i started working in a histology lab, run only by me ( coworkers are not specialized in histology). There has not worked here a person at histology for about 2 years. After many new protocols, i decided to clear out some chemicals. Now i found around 1 KG of DRY picric acid. I informed my coworkers about this, and they said just to dissolve everything in water. What do you guys think is the best way for handeling with this explosive chemical? Thank you all in advance! Maarten From amurvosh at advancederm.net Thu May 5 14:19:22 2016 From: amurvosh at advancederm.net (Anne Murvosh) Date: Thu, 5 May 2016 19:19:22 +0000 Subject: [Histonet] PAS Stain In-Reply-To: References: <1015261985.24592150.1462472828158.JavaMail.zimbra@comcast.net> Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7B00E64@Exchange.Advancederm.net> Yes, spitting is the tried and true way to do it. Not to mention no measuring and cheaper. The reason we switched to a powder is because I just don't spit well I used to have someone do it for me cause I would end up drooling. YUCK! The best way to find out is do the amylase method and the spit method at the same time and have the doctor pick the best. A fun experiment Anne -----Original Message----- From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 11:36 AM To: koellingr at comcast.net Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain Spokane Ray points out something I've wondered about for years - can just anybody spit on the slide and remove the glycogen? I've never heard of any variation, but the number of people I've asked is very limited. This reference: http://www.ncbi.nlm.nih.gov/gene/276 certainly suggests that different people have different salivary alpha amylase activity. Bob Richmond On Thu, May 5, 2016 at 2:27 PM, wrote: > I love having the Samuri Pathologist on this forum for wisdom and > real-laboratory life knowledge. And yes, I have in the past spit on slide > ON OCCASSION when faced with a dire necessity. Although I know there are > those who would wretch about this; it remains a fact of viable laboratory > life for some. > > My problem now is that in this era of (MUCH TOO MUCH) regulation, how do > you "test lots" or control from "lot-to-lot variation" in this SOP? When > Jane or Joe do this routinely and then goes on vacation, what about Sally > or Jim spit? There is a variation in copy number of the AMY1 gene > (amylase) and resulting difference in amylase protein concentration amongst > individuals. > > Why not just standardize it from the start, reagent, pH, temperature and > it really cannot fail. > > Spokane Ray > > ------------------------------ > *From: *"Bob Richmond via Histonet" > *To: *"Histonet at lists.utsouthwestern.edu" < > histonet at lists.utsouthwestern.edu> > *Sent: *Thursday, May 5, 2016 11:10:40 AM > *Subject: *Re: [Histonet] PAS Stain > > > Amylase (diastase) for the PAS stain queries: > > Whatever happened to spitting on the slide (30 min at room temperature)? > John Kiernan advises "thinking of lemons and drooling into a small beaker" > though I'd advise chewing on a rubber band for a few seconds. > > He notes that alpha amylase is preferred. I'd go with the cheapest one in > the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma > offers a heat-stable alpha amylase. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SPINHEIRO at lumc.edu Thu May 5 14:29:12 2016 From: SPINHEIRO at lumc.edu (STEVEN PINHEIRO) Date: Thu, 5 May 2016 19:29:12 +0000 Subject: [Histonet] picric acid In-Reply-To: References: Message-ID: Well you have the most important part correct- Keep it wet. But that doesn't address how to dispose of it. You could choose to make it non explosive by using sodium hydroxide and sulfide. But then its toxic and needs to be treated and removed accordingly. You can also list it as flammable and have it removed by the correctly licensed third party. Never pour down the drain as it could react w copper pipes. Many reasons to dispose of it properly. Steve Pinheiro -----Original Message----- From: Mca Werdler via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 2:09 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] picric acid Dear histonetters, Since a few months, i started working in a histology lab, run only by me ( coworkers are not specialized in histology). There has not worked here a person at histology for about 2 years. After many new protocols, i decided to clear out some chemicals. Now i found around 1 KG of DRY picric acid. I informed my coworkers about this, and they said just to dissolve everything in water. What do you guys think is the best way for handeling with this explosive chemical? Thank you all in advance! Maarten _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From mcauliff at rwjms.rutgers.edu Thu May 5 14:31:12 2016 From: mcauliff at rwjms.rutgers.edu (Geoff) Date: Thu, 05 May 2016 15:31:12 -0400 Subject: [Histonet] PAS Stain In-Reply-To: <22BDD9AABC13E24E95D1CF064B75C4B7B00E64@Exchange.Advancederm.net> References: <1015261985.24592150.1462472828158.JavaMail.zimbra@comcast.net> <22BDD9AABC13E24E95D1CF064B75C4B7B00E64@Exchange.Advancederm.net> Message-ID: <572B9F80.9070803@umdnj.edu> I cannot believe any scientist would advocate such a non-uniform method as spitting on a slide. Buy a bottle of what ever enzyme and use a reproducible buffer and temperature. Geoff On 5/5/2016 3:19 PM, Anne Murvosh via Histonet wrote: > Yes, spitting is the tried and true way to do it. Not to mention no measuring and cheaper. The reason we switched to a powder is because I just don't spit well I used to have someone do it for me cause I would end up drooling. YUCK! The best way to find out is do the amylase method and the spit method at the same time and have the doctor pick the best. A fun experiment Anne > > -----Original Message----- > From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, May 05, 2016 11:36 AM > To: koellingr at comcast.net > Cc: Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] PAS Stain > > Spokane Ray points out something I've wondered about for years - can just > anybody spit on the slide and remove the glycogen? I've never heard of any > variation, but the number of people I've asked is very limited. This > reference: > http://www.ncbi.nlm.nih.gov/gene/276 > certainly suggests that different people have different salivary alpha > amylase activity. > > Bob Richmond > > On Thu, May 5, 2016 at 2:27 PM, wrote: > >> I love having the Samuri Pathologist on this forum for wisdom and >> real-laboratory life knowledge. And yes, I have in the past spit on slide >> ON OCCASSION when faced with a dire necessity. Although I know there are >> those who would wretch about this; it remains a fact of viable laboratory >> life for some. >> >> My problem now is that in this era of (MUCH TOO MUCH) regulation, how do >> you "test lots" or control from "lot-to-lot variation" in this SOP? When >> Jane or Joe do this routinely and then goes on vacation, what about Sally >> or Jim spit? There is a variation in copy number of the AMY1 gene >> (amylase) and resulting difference in amylase protein concentration amongst >> individuals. >> >> Why not just standardize it from the start, reagent, pH, temperature and >> it really cannot fail. >> >> Spokane Ray >> >> ------------------------------ >> *From: *"Bob Richmond via Histonet" >> *To: *"Histonet at lists.utsouthwestern.edu" < >> histonet at lists.utsouthwestern.edu> >> *Sent: *Thursday, May 5, 2016 11:10:40 AM >> *Subject: *Re: [Histonet] PAS Stain >> >> >> Amylase (diastase) for the PAS stain queries: >> >> Whatever happened to spitting on the slide (30 min at room temperature)? >> John Kiernan advises "thinking of lemons and drooling into a small beaker" >> though I'd advise chewing on a rubber band for a few seconds. >> >> He notes that alpha amylase is preferred. I'd go with the cheapest one in >> the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma >> offers a heat-stable alpha amylase. >> >> Bob Richmond >> Samurai Pathologist >> Maryville TN >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff at rwjms.rutgers.edu ********************************************** From CIngles at uwhealth.org Thu May 5 14:55:01 2016 From: CIngles at uwhealth.org (Ingles Claire) Date: Thu, 5 May 2016 19:55:01 +0000 Subject: [Histonet] PAS Stain In-Reply-To: <922426283.24607846.1462474095938.JavaMail.zimbra@comcast.net> References: <1015261985.24592150.1462472828158.JavaMail.zimbra@comcast.net> , <922426283.24607846.1462474095938.JavaMail.zimbra@comcast.net> Message-ID: The level of amylase in your saliva also depends on when you ate last. If you're spitting on slides after you just ate, the reaction will be weaker as the amylase will have been used up on lunch digestion. (also, you need to be careful about having your lunch salad contaminate the slide... :) Claire ________________________________________ From: Ray via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 1:48 PM To: Richmond, Bob Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain An excellent point. For anyone wanting to investigate-simply do a PubMed search on variation of AMY1 gene. Sorry; I guess I should say this is, strictly speaking, non-histology related topic and I don't want to get into trouble as some before me. Tons of research about this linking back (in theory) to positive selection in hunter-gatherers versus agricultural ancestors, race, obesity, phenotypic and dietary differences as to why maybe there can be big differences. Spokane Ray ----- Original Message ----- From: "Bob Richmond" To: koellingr at comcast.net Cc: "Histonet at lists.utsouthwestern.edu" Sent: Thursday, May 5, 2016 11:35:42 AM Subject: Re: [Histonet] PAS Stain Spokane Ray points out something I've wondered about for years - can just anybody spit on the slide and remove the glycogen? I've never heard of any variation, but the number of people I've asked is very limited. This reference: http://www.ncbi.nlm.nih.gov/gene/276 certainly suggests that different people have different salivary alpha amylase activity. Bob Richmond On Thu, May 5, 2016 at 2:27 PM, < koellingr at comcast.net > wrote: I love having the Samuri Pathologist on this forum for wisdom and real-laboratory life knowledge. And yes, I have in the past spit on slide ON OCCASSION when faced with a dire necessity. Although I know there are those who would wretch about this; it remains a fact of viable laboratory life for some. My problem now is that in this era of (MUCH TOO MUCH) regulation, how do you "test lots" or control from "lot-to-lot variation" in this SOP? When Jane or Joe do this routinely and then goes on vacation, what about Sally or Jim spit? There is a variation in copy number of the AMY1 gene (amylase) and resulting difference in amylase protein concentration amongst individuals. Why not just standardize it from the start, reagent, pH, temperature and it really cannot fail. Spokane Ray From: "Bob Richmond via Histonet" < histonet at lists.utsouthwestern.edu > To: " Histonet at lists.utsouthwestern.edu " < histonet at lists.utsouthwestern.edu > Sent: Thursday, May 5, 2016 11:10:40 AM Subject: Re: [Histonet] PAS Stain Amylase (diastase) for the PAS stain queries: Whatever happened to spitting on the slide (30 min at room temperature)? John Kiernan advises "thinking of lemons and drooling into a small beaker" though I'd advise chewing on a rubber band for a few seconds. He notes that alpha amylase is preferred. I'd go with the cheapest one in the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma offers a heat-stable alpha amylase. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren at gmail.com Thu May 5 15:02:05 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 5 May 2016 13:02:05 -0700 Subject: [Histonet] picric acid In-Reply-To: References: Message-ID: https://www.youtube.com/watch?v=IqTZNn0UHdA Jay A. Lundgren, M.S., HTL (ASCP) On Thu, May 5, 2016 at 12:29 PM, STEVEN PINHEIRO via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Well you have the most important part correct- Keep it wet. But that > doesn't address how to dispose of it. You could choose to make it non > explosive by using sodium hydroxide and sulfide. But then its toxic and > needs to be treated and removed accordingly. You can also list it as > flammable and have it removed by the correctly licensed third party. > Never pour down the drain as it could react w copper pipes. Many reasons > to dispose of it properly. > > Steve Pinheiro > > > > -----Original Message----- > From: Mca Werdler via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, May 05, 2016 2:09 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] picric acid > > Dear histonetters, > > Since a few months, i started working in a histology lab, run only by me ( > coworkers are not specialized in histology). There has not worked here a > person at histology for about 2 years. > > After many new protocols, i decided to clear out some chemicals. > Now i found around 1 KG of DRY picric acid. I informed my coworkers about > this, and they said just to dissolve everything in water. > > What do you guys think is the best way for handeling with this explosive > chemical? Thank you all in advance! > > Maarten > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amurvosh at advancederm.net Thu May 5 15:02:31 2016 From: amurvosh at advancederm.net (Anne Murvosh) Date: Thu, 5 May 2016 20:02:31 +0000 Subject: [Histonet] PAS Stain In-Reply-To: <572B9F80.9070803@umdnj.edu> References: <1015261985.24592150.1462472828158.JavaMail.zimbra@comcast.net> <22BDD9AABC13E24E95D1CF064B75C4B7B00E64@Exchange.Advancederm.net> <572B9F80.9070803@umdnj.edu> Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7B00EA0@Exchange.Advancederm.net> You clearly don't know your histo history. The reason we know that H pylori exists is because a Scientist, Dr. Barry Marshall wanted to prove bacteria caused ulcers and not stress. No one believed him. So he took the organisms from a patient, mixed it in a broth and drank it. He then biopsied himself and treated it. There's a non-uniform method that saved a lot of suffering. Bravo we crazy scientists. Anne -----Original Message----- From: Geoff via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 12:31 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain I cannot believe any scientist would advocate such a non-uniform method as spitting on a slide. Buy a bottle of what ever enzyme and use a reproducible buffer and temperature. Geoff On 5/5/2016 3:19 PM, Anne Murvosh via Histonet wrote: > Yes, spitting is the tried and true way to do it. Not to mention no measuring and cheaper. The reason we switched to a powder is because I just don't spit well I used to have someone do it for me cause I would end up drooling. YUCK! The best way to find out is do the amylase method and the spit method at the same time and have the doctor pick the best. A fun experiment Anne > > -----Original Message----- > From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, May 05, 2016 11:36 AM > To: koellingr at comcast.net > Cc: Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] PAS Stain > > Spokane Ray points out something I've wondered about for years - can just > anybody spit on the slide and remove the glycogen? I've never heard of any > variation, but the number of people I've asked is very limited. This > reference: > http://www.ncbi.nlm.nih.gov/gene/276 > certainly suggests that different people have different salivary alpha > amylase activity. > > Bob Richmond > > On Thu, May 5, 2016 at 2:27 PM, wrote: > >> I love having the Samuri Pathologist on this forum for wisdom and >> real-laboratory life knowledge. And yes, I have in the past spit on slide >> ON OCCASSION when faced with a dire necessity. Although I know there are >> those who would wretch about this; it remains a fact of viable laboratory >> life for some. >> >> My problem now is that in this era of (MUCH TOO MUCH) regulation, how do >> you "test lots" or control from "lot-to-lot variation" in this SOP? When >> Jane or Joe do this routinely and then goes on vacation, what about Sally >> or Jim spit? There is a variation in copy number of the AMY1 gene >> (amylase) and resulting difference in amylase protein concentration amongst >> individuals. >> >> Why not just standardize it from the start, reagent, pH, temperature and >> it really cannot fail. >> >> Spokane Ray >> >> ------------------------------ >> *From: *"Bob Richmond via Histonet" >> *To: *"Histonet at lists.utsouthwestern.edu" < >> histonet at lists.utsouthwestern.edu> >> *Sent: *Thursday, May 5, 2016 11:10:40 AM >> *Subject: *Re: [Histonet] PAS Stain >> >> >> Amylase (diastase) for the PAS stain queries: >> >> Whatever happened to spitting on the slide (30 min at room temperature)? >> John Kiernan advises "thinking of lemons and drooling into a small beaker" >> though I'd advise chewing on a rubber band for a few seconds. >> >> He notes that alpha amylase is preferred. I'd go with the cheapest one in >> the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma >> offers a heat-stable alpha amylase. >> >> Bob Richmond >> Samurai Pathologist >> Maryville TN >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff at rwjms.rutgers.edu ********************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum at uams.edu Thu May 5 16:07:03 2016 From: PAMarcum at uams.edu (Marcum, Pamela A) Date: Thu, 5 May 2016 21:07:03 +0000 Subject: [Histonet] PAS/Decal Question In-Reply-To: <002501d1a69e$6f8bede0$4ea3c9a0$@gmx.at> References: <826681eae65c40b28de6f620b3207f05@MAIL13M2N1.ad.uams.edu> <002501d1a69e$6f8bede0$4ea3c9a0$@gmx.at> Message-ID: We have no say in the decal procedure and I have been fighting this for 6 years. It is determined by our HemePath docs and when they want bone marrows completed for clinics the next day. We have a 24 hour turnaround time, which is ridiculous however; we cannot not change it. The feedback we get now is the PAS stain on the bone marrows Levels 2 and 4 are not staining evenly. They show a smudginess in some cells on the lower or level 4 section/ or they look over processed/cooked. WE have done manual, instrument and now Dako Artisan (Dako has bend over backward to help) and nothing changes what they see on the slides. They will not even consider changing the early steps as I have been told decal is fine (no its not) and processing is fine. Funny thing H&Es are always fine and only the PAS is a problem. Just tired of it. This has been a losing fight for almost 5 months and I reached out to all of you in hopes it would help us make some changes. Thank you for your suggestions unfortunately no one wants to hear about changes if they mean it is not a 24 hour turnaround time. I am almost ready to just say "garbage in garbage out". I feel very bad for the patients at this point. I have not given up just trying to find a way to get change and better results. Thank You All for Your Suggestions!! Pam -----Original Message----- From: Gudrun Lang [mailto:gu.lang at gmx.at] Sent: Thursday, May 05, 2016 2:19 AM To: Marcum, Pamela A Cc: histonet at lists.utsouthwestern.edu Subject: AW: [Histonet] PAS/Decal Question Hi Pam, my personal opinion is, that 2 hours fixation is too short for sufficient tissue-protection before acid decalcification. Formic acid at 50?C must have an impact on glycoproteins. Wether it is a kind of solving the "sugars" or beginning oxidation of the OH-groups (like periodic acid does in the PAS-reaction). In our lab we do also acid decal with formic acid for at least 6 hours at RT, after one day in NBF. Our processing protocol is the routine-protocol over night. How thick are your BMT, also 3-4 mm? In my opinion 4 hours are a challenge. Are the other stainings of the BMT optimal or show sometimes similar outcome? "Smudginess" reminds me of insufficient infiltration. I also see that our PAS is not as bright as in the other specimens without decal. Sometimes it gives more the impression of a diastase-PAS. Gudrun -----Urspr?ngliche Nachricht----- Von: Marcum, Pamela A via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Dienstag, 03. Mai 2016 18:39 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] PAS/Decal Question We are still having issues with our PAS stain on decaled bone marrows. The Pathologists in HemePath are seeing what they refer to as smudginess in cells on some areas of the completed PAS slides. We have looked at everything and cannot find where the issue is coming from at this point. We have done manual staining for PAS, automated on the Leica stainer and on the Dako Artisan. All methods show the same result for some slides. We can go for several days to a week or more with no problem and then suddenly it is back and we have changed nothing in the way we do the processing, embedding, sectioning, deparaffinization and coverslipping. We do as many as 38 bone marrow cores a night or as few as 8 and can find no correlation in the number we have to deal with for a given period. All bone marrows drawn today must be completed by 8AM tomorrow morning. Fixation after pulling the bone marrows is a minimum of 2 hours in AZF with a maximum of 7 hours +/-. Grossed and placed in cassettes for 15 minute rinse in running DI Water Decal currently in the Milestone Decal Unit for 45 minutes in Immunocal at 50C Rinsed in running DI water for 15 minutes Placed in 10% NBF and processed on a 4 hour program with a delay of 4 hours minimum to come off at 4:45AM. If anyone knows of any literature on decal effects on PAS staining in bone marrows please contact me. This has been going on for months and no matter what we do manual staining, Leica adaptation for automated or Dako it is not helping. Dako has been great with sending in technical experts repeatedly and we cannot get this corrected. Thanks, Pam ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfields at mlkch.org Thu May 5 16:59:57 2016 From: cfields at mlkch.org (Carol G Fields) Date: Thu, 5 May 2016 21:59:57 +0000 Subject: [Histonet] picric acid In-Reply-To: References: Message-ID: <2d2437d2de114596a73f4db82ec0d215@mlk01exc02.mlkch.org> Hi, Do not touch it and call the bomb squad to dispose of it. I have had several friends, including myself, find this on a back shelf in Histology and when they hauled it off and exploded it they said it would take out a city block. I do not mess with it..... it's not worth taking the chance. Call the proper people to dispose of it for you. Carole Los Angeles, CA ? -----Original Message----- From: Mca Werdler via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 12:09 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] picric acid Dear histonetters, Since a few months, i started working in a histology lab, run only by me ( coworkers are not specialized in histology). There has not worked here a person at histology for about 2 years. After many new protocols, i decided to clear out some chemicals. Now i found around 1 KG of DRY picric acid. I informed my coworkers about this, and they said just to dissolve everything in water. What do you guys think is the best way for handeling with this explosive chemical? Thank you all in advance! Maarten _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email message and any files transmitted are sent with confidentiality in mind and contain privileged or copyright information. You must not present this message to another party without gaining permission from the sender. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. Any views expressed in this message are those of the sender, except where the sender specifically states them to be the views of Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. If you have received this message in error, please notify the sender immediately, and delete this email from your system. We do not guarantee that this material is free from viruses or any other defects although due care has been taken to minimize the risk. From gachstetter at yahoo.com Thu May 5 17:45:39 2016 From: gachstetter at yahoo.com (Ginny Achstetter) Date: Thu, 5 May 2016 18:45:39 -0400 Subject: [Histonet] Slide printers Message-ID: <337FE93F-A1F6-47B3-A7F7-7C63AA9462D9@yahoo.com> I need a recommendation on a slide printer. Tried the Leica and liked it but it only works well with their round edged slides and they aren't charged. Sent from my iPhone From tony.henwood at health.nsw.gov.au Thu May 5 18:30:57 2016 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 5 May 2016 23:30:57 +0000 Subject: [Histonet] PAS Stain Message-ID: <0237449DE79DBC45B686AB82CDCD16FF01498E7E@SVDCMBX-MEX008.nswhealth.net> Yep, I have had a few techs in my time who could not digest glycogen if their life depended on it. Their salivary amylase just did not work. It was not a major (not even a minor) health issue for them. They looked healthy enough (actually healthier than me). This is one of the reasons we developed a 10 minute room temp amylase method: Mangan, V-M, Farago, V., Kelly, M., Henwood, A.F., (2002) "An Amylase Reagent with a Long Shelf Life for the removal of Glycogen from Tissue Sections" J. Histotechnol 25:153. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Ray via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 6 May 2016 4:48 AM To: Richmond, Bob Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain An excellent point.? For anyone wanting to investigate-simply do a PubMed search on variation of AMY1 gene.? Sorry; I?guess I should say this is, strictly speaking, non-histology related topic and I don't want to get into trouble as some before me.? Tons of research about this linking back (in theory) to positive selection in hunter-gatherers versus agricultural ancestors, race, obesity, phenotypic and dietary differences as to why maybe there can be big differences. Spokane Ray ----- Original Message ----- From: "Bob Richmond" To: koellingr at comcast.net Cc: "Histonet at lists.utsouthwestern.edu" Sent: Thursday, May 5, 2016 11:35:42 AM Subject: Re: [Histonet] PAS Stain Spokane Ray points out something I've wondered about for years - can just anybody spit on the slide and remove the glycogen? I've never heard of any variation, but the number of people I've asked is very limited. This reference: http://www.ncbi.nlm.nih.gov/gene/276 certainly suggests that different people have different salivary alpha amylase activity. Bob Richmond On Thu, May 5, 2016 at 2:27 PM, < koellingr at comcast.net > wrote: I love having the Samuri Pathologist on this forum for wisdom and real-laboratory life knowledge.? And yes, I have in the past spit on slide ON OCCASSION when faced with a dire necessity.? Although I know there are those who would wretch about this; it remains a fact of viable laboratory life for some. ? My problem now is that in this era of (MUCH TOO MUCH) regulation, how do you "test lots" or control from "lot-to-lot variation" in this SOP?? When Jane or Joe do this routinely and then goes on vacation, what about Sally or Jim spit?? There is a variation in copy number of the AMY1 gene (amylase) and resulting difference in amylase protein concentration amongst individuals. ? Why not just standardize it from the start, reagent, pH, temperature and it really cannot fail. ? Spokane Ray From: "Bob Richmond via Histonet" < histonet at lists.utsouthwestern.edu > To: " Histonet at lists.utsouthwestern.edu " < histonet at lists.utsouthwestern.edu > Sent: Thursday, May 5, 2016 11:10:40 AM Subject: Re: [Histonet] PAS Stain Amylase (diastase) for the PAS stain queries: Whatever happened to spitting on the slide (30 min at room temperature)? John Kiernan advises "thinking of lemons and drooling into a small beaker" though I'd advise chewing on a rubber band for a few seconds. He notes that alpha amylase is preferred. I'd go with the cheapest one in the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma offers a heat-stable alpha amylase. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From tony.henwood at health.nsw.gov.au Thu May 5 18:33:59 2016 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 5 May 2016 23:33:59 +0000 Subject: [Histonet] PAS Stain In-Reply-To: <22BDD9AABC13E24E95D1CF064B75C4B7B00EA0@Exchange.Advancederm.net> References: <1015261985.24592150.1462472828158.JavaMail.zimbra@comcast.net> <22BDD9AABC13E24E95D1CF064B75C4B7B00E64@Exchange.Advancederm.net> <572B9F80.9070803@umdnj.edu> <22BDD9AABC13E24E95D1CF064B75C4B7B00EA0@Exchange.Advancederm.net> Message-ID: <0237449DE79DBC45B686AB82CDCD16FF01498EA0@SVDCMBX-MEX008.nswhealth.net> Only a crazy Aussie would do this!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Anne Murvosh via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 6 May 2016 6:03 AM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain You clearly don't know your histo history. The reason we know that H pylori exists is because a Scientist, Dr. Barry Marshall wanted to prove bacteria caused ulcers and not stress. No one believed him. So he took the organisms from a patient, mixed it in a broth and drank it. He then biopsied himself and treated it. There's a non-uniform method that saved a lot of suffering. Bravo we crazy scientists. Anne -----Original Message----- From: Geoff via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 12:31 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain I cannot believe any scientist would advocate such a non-uniform method as spitting on a slide. Buy a bottle of what ever enzyme and use a reproducible buffer and temperature. Geoff On 5/5/2016 3:19 PM, Anne Murvosh via Histonet wrote: > Yes, spitting is the tried and true way to do it. Not to mention no measuring and cheaper. The reason we switched to a powder is because I just don't spit well I used to have someone do it for me cause I would end up drooling. YUCK! The best way to find out is do the amylase method and the spit method at the same time and have the doctor pick the best. A fun experiment Anne > > -----Original Message----- > From: Bob Richmond via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, May 05, 2016 11:36 AM > To: koellingr at comcast.net > Cc: Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] PAS Stain > > Spokane Ray points out something I've wondered about for years - can > just anybody spit on the slide and remove the glycogen? I've never > heard of any variation, but the number of people I've asked is very > limited. This > reference: > http://www.ncbi.nlm.nih.gov/gene/276 > certainly suggests that different people have different salivary alpha > amylase activity. > > Bob Richmond > > On Thu, May 5, 2016 at 2:27 PM, wrote: > >> I love having the Samuri Pathologist on this forum for wisdom and >> real-laboratory life knowledge. And yes, I have in the past spit on >> slide ON OCCASSION when faced with a dire necessity. Although I know >> there are those who would wretch about this; it remains a fact of >> viable laboratory life for some. >> >> My problem now is that in this era of (MUCH TOO MUCH) regulation, how >> do you "test lots" or control from "lot-to-lot variation" in this >> SOP? When Jane or Joe do this routinely and then goes on vacation, >> what about Sally or Jim spit? There is a variation in copy number of >> the AMY1 gene >> (amylase) and resulting difference in amylase protein concentration >> amongst individuals. >> >> Why not just standardize it from the start, reagent, pH, temperature >> and it really cannot fail. >> >> Spokane Ray >> >> ------------------------------ >> *From: *"Bob Richmond via Histonet" >> >> *To: *"Histonet at lists.utsouthwestern.edu" < >> histonet at lists.utsouthwestern.edu> >> *Sent: *Thursday, May 5, 2016 11:10:40 AM >> *Subject: *Re: [Histonet] PAS Stain >> >> >> Amylase (diastase) for the PAS stain queries: >> >> Whatever happened to spitting on the slide (30 min at room temperature)? >> John Kiernan advises "thinking of lemons and drooling into a small beaker" >> though I'd advise chewing on a rubber band for a few seconds. >> >> He notes that alpha amylase is preferred. I'd go with the cheapest >> one in the Sigma-Aldrich catalog. Room temperature is usual, but I >> note that Sigma offers a heat-stable alpha amylase. >> >> Bob Richmond >> Samurai Pathologist >> Maryville TN >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff at rwjms.rutgers.edu ********************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From john.frazier at roche.com Thu May 5 18:34:32 2016 From: john.frazier at roche.com (Frazier, John) Date: Thu, 5 May 2016 19:34:32 -0400 Subject: [Histonet] Slide printers In-Reply-To: <337FE93F-A1F6-47B3-A7F7-7C63AA9462D9@yahoo.com> References: <337FE93F-A1F6-47B3-A7F7-7C63AA9462D9@yahoo.com> Message-ID: <-4254020579740761983@unknownmsgid> The Thermo Slidemate and Prima are both good. The Thermo is small. It is designed for a microtome work station. If you find another company, I would be careful to ensure they can interface with your LIS and or cassette marker. You want continuity between the two makers and the LIS Sent from my iPad > On May 5, 2016, at 6:45 PM, Ginny Achstetter wrote: > > > I need a recommendation on a slide printer. Tried the Leica and liked it but it only works well with their round edged slides and they aren't charged. > Sent from my iPhone > > From CIngles at uwhealth.org Thu May 5 19:06:47 2016 From: CIngles at uwhealth.org (Ingles Claire) Date: Fri, 6 May 2016 00:06:47 +0000 Subject: [Histonet] PAS Stain In-Reply-To: <0237449DE79DBC45B686AB82CDCD16FF01498EA0@SVDCMBX-MEX008.nswhealth.net> References: <1015261985.24592150.1462472828158.JavaMail.zimbra@comcast.net> <22BDD9AABC13E24E95D1CF064B75C4B7B00E64@Exchange.Advancederm.net> <572B9F80.9070803@umdnj.edu> <22BDD9AABC13E24E95D1CF064B75C4B7B00EA0@Exchange.Advancederm.net>, <0237449DE79DBC45B686AB82CDCD16FF01498EA0@SVDCMBX-MEX008.nswhealth.net> Message-ID: I don't know, I believe Dr. Salk did the same with the polio vaccine. He even involved his family! Dedicated doctors... Claire ________________________________________ From: Tony Henwood (SCHN) via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 6:33 PM To: Anne Murvosh Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain Only a crazy Aussie would do this!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Anne Murvosh via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 6 May 2016 6:03 AM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain You clearly don't know your histo history. The reason we know that H pylori exists is because a Scientist, Dr. Barry Marshall wanted to prove bacteria caused ulcers and not stress. No one believed him. So he took the organisms from a patient, mixed it in a broth and drank it. He then biopsied himself and treated it. There's a non-uniform method that saved a lot of suffering. Bravo we crazy scientists. Anne -----Original Message----- From: Geoff via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 12:31 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain I cannot believe any scientist would advocate such a non-uniform method as spitting on a slide. Buy a bottle of what ever enzyme and use a reproducible buffer and temperature. Geoff On 5/5/2016 3:19 PM, Anne Murvosh via Histonet wrote: > Yes, spitting is the tried and true way to do it. Not to mention no measuring and cheaper. The reason we switched to a powder is because I just don't spit well I used to have someone do it for me cause I would end up drooling. YUCK! The best way to find out is do the amylase method and the spit method at the same time and have the doctor pick the best. A fun experiment Anne > > -----Original Message----- > From: Bob Richmond via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, May 05, 2016 11:36 AM > To: koellingr at comcast.net > Cc: Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] PAS Stain > > Spokane Ray points out something I've wondered about for years - can > just anybody spit on the slide and remove the glycogen? I've never > heard of any variation, but the number of people I've asked is very > limited. This > reference: > http://www.ncbi.nlm.nih.gov/gene/276 > certainly suggests that different people have different salivary alpha > amylase activity. > > Bob Richmond > > On Thu, May 5, 2016 at 2:27 PM, wrote: > >> I love having the Samuri Pathologist on this forum for wisdom and >> real-laboratory life knowledge. And yes, I have in the past spit on >> slide ON OCCASSION when faced with a dire necessity. Although I know >> there are those who would wretch about this; it remains a fact of >> viable laboratory life for some. >> >> My problem now is that in this era of (MUCH TOO MUCH) regulation, how >> do you "test lots" or control from "lot-to-lot variation" in this >> SOP? When Jane or Joe do this routinely and then goes on vacation, >> what about Sally or Jim spit? There is a variation in copy number of >> the AMY1 gene >> (amylase) and resulting difference in amylase protein concentration >> amongst individuals. >> >> Why not just standardize it from the start, reagent, pH, temperature >> and it really cannot fail. >> >> Spokane Ray >> >> ------------------------------ >> *From: *"Bob Richmond via Histonet" >> >> *To: *"Histonet at lists.utsouthwestern.edu" < >> histonet at lists.utsouthwestern.edu> >> *Sent: *Thursday, May 5, 2016 11:10:40 AM >> *Subject: *Re: [Histonet] PAS Stain >> >> >> Amylase (diastase) for the PAS stain queries: >> >> Whatever happened to spitting on the slide (30 min at room temperature)? >> John Kiernan advises "thinking of lemons and drooling into a small beaker" >> though I'd advise chewing on a rubber band for a few seconds. >> >> He notes that alpha amylase is preferred. I'd go with the cheapest >> one in the Sigma-Aldrich catalog. Room temperature is usual, but I >> note that Sigma offers a heat-stable alpha amylase. >> >> Bob Richmond >> Samurai Pathologist >> Maryville TN >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff at rwjms.rutgers.edu ********************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotex at sbcglobal.net Thu May 5 19:30:22 2016 From: histotex at sbcglobal.net (Sherry Martin) Date: Fri, 6 May 2016 00:30:22 +0000 (UTC) Subject: [Histonet] PAS Stain In-Reply-To: References: Message-ID: <1505795437.73087.1462494622925.JavaMail.yahoo@mail.yahoo.com> Hello All! I've served in laboratory medicine for well over 35 years and in my early years we did indeed spit on the slides. I learned very quickly on that my personal spit fails to digest any glycogen. And worse yet, I used to have to search for someone else to spit for me. PAS stains were always awkward and gross! I'm VERY thankful for the artificial amylase we have now. :-) Y'all have a great day! Sherry Martin On Thursday, May 5, 2016 7:06 PM, Ingles Claire via Histonet wrote: I don't know, I believe Dr. Salk did the same with the polio vaccine. He even involved his family! Dedicated doctors... Claire ________________________________________ From: Tony Henwood (SCHN) via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 6:33 PM To: Anne Murvosh Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain Only a crazy Aussie would do this!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Anne Murvosh via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 6 May 2016 6:03 AM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain You clearly don't know your histo history. The reason we know that H pylori exists is because a Scientist, Dr. Barry Marshall wanted to prove bacteria caused ulcers and not stress.? No one believed him.? So he took the organisms from a patient, mixed it in a broth and drank it. He then biopsied himself and treated it. There's a non-uniform method that saved a lot of suffering.? Bravo we crazy scientists.? Anne -----Original Message----- From: Geoff via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 12:31 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain I cannot believe any scientist would advocate such a non-uniform method as spitting on a slide. Buy a bottle of what ever enzyme and use a reproducible buffer and temperature. Geoff On 5/5/2016 3:19 PM, Anne Murvosh via Histonet wrote: > Yes, spitting is the tried and true way to do it.? Not to mention no measuring and cheaper.? The reason we switched to a powder is because I just don't spit well I used to have someone do it for me cause I would end up drooling. YUCK! The best way to find out is do the amylase method and the spit method at the same time and have the doctor pick the best.? A fun experiment? Anne > > -----Original Message----- > From: Bob Richmond via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, May 05, 2016 11:36 AM > To: koellingr at comcast.net > Cc: Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] PAS Stain > > Spokane Ray points out something I've wondered about for years - can > just anybody spit on the slide and remove the glycogen? I've never > heard of any variation, but the number of people I've asked is very > limited. This > reference: > http://www.ncbi.nlm.nih.gov/gene/276 > certainly suggests that different people have different salivary alpha > amylase activity. > > Bob Richmond > > On Thu, May 5, 2016 at 2:27 PM, wrote: > >> I love having the Samuri Pathologist on this forum for wisdom and >> real-laboratory life knowledge.? And yes, I have in the past spit on >> slide ON OCCASSION when faced with a dire necessity.? Although I know >> there are those who would wretch about this; it remains a fact of >> viable laboratory life for some. >> >> My problem now is that in this era of (MUCH TOO MUCH) regulation, how >> do you "test lots" or control from "lot-to-lot variation" in this >> SOP?? When Jane or Joe do this routinely and then goes on vacation, >> what about Sally or Jim spit?? There is a variation in copy number of >> the AMY1 gene >> (amylase) and resulting difference in amylase protein concentration >> amongst individuals. >> >> Why not just standardize it from the start, reagent, pH, temperature >> and it really cannot fail. >> >> Spokane Ray >> >> ------------------------------ >> *From: *"Bob Richmond via Histonet" >> >> *To: *"Histonet at lists.utsouthwestern.edu" < >> histonet at lists.utsouthwestern.edu> >> *Sent: *Thursday, May 5, 2016 11:10:40 AM >> *Subject: *Re: [Histonet] PAS Stain >> >> >> Amylase (diastase) for the PAS stain queries: >> >> Whatever happened to spitting on the slide (30 min at room temperature)? >> John Kiernan advises "thinking of lemons and drooling into a small beaker" >> though I'd advise chewing on a rubber band for a few seconds. >> >> He notes that alpha amylase is preferred. I'd go with the cheapest >> one in the Sigma-Aldrich catalog. Room temperature is usual, but I >> note that Sigma offers a heat-stable alpha amylase. >> >> Bob Richmond >> Samurai Pathologist >> Maryville TN >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff at rwjms.rutgers.edu ********************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr at comcast.net Thu May 5 20:54:37 2016 From: koellingr at comcast.net (koellingr at comcast.net) Date: Fri, 6 May 2016 01:54:37 +0000 (UTC) Subject: [Histonet] PAS Stain In-Reply-To: <1505795437.73087.1462494622925.JavaMail.yahoo@mail.yahoo.com> References: <1505795437.73087.1462494622925.JavaMail.yahoo@mail.yahoo.com> Message-ID: <2010988384.24876514.1462499677121.JavaMail.zimbra@comcast.net> Indeed, a very curious and interesting way to do this.? And as I said-have done it very occasionally in long ago past. What I am still curious about is that for those who still do this, how do you write it up for CLIA or CAP or GLP when, as the Samuri Pathologist would call them, Herrn Inspektors come to visit.? Lot number of spit?? Variation of?spit reagent?with a different lot (person)?? Preparation of spit reagent (before or after starchy meal)?? What the step-by-step procedure looks like?? Just curious. ? Spokane Ray ----- Original Message ----- From: "Sherry Martin via Histonet" To: histonet at lists.utsouthwestern.edu Sent: Thursday, May 5, 2016 5:30:22 PM Subject: Re: [Histonet] PAS Stain Hello All! I've served in laboratory medicine for well over 35 years and in my early years we did indeed spit on the slides. I learned very quickly on that my personal spit fails to digest any glycogen. And worse yet, I used to have to search for someone else to spit for me. PAS stains were always awkward and gross! I'm VERY thankful for the artificial amylase we have now. :-) Y'all have a great day! Sherry Martin ?? ?On Thursday, May 5, 2016 7:06 PM, Ingles Claire via Histonet wrote: ? ?I don't know, I believe Dr. Salk did the same with the polio vaccine. He even involved his family! Dedicated doctors... Claire ________________________________________ From: Tony Henwood (SCHN) via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 6:33 PM To: Anne Murvosh Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain Only a crazy Aussie would do this!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Anne Murvosh via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 6 May 2016 6:03 AM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain You clearly don't know your histo history. The reason we know that H pylori exists is because a Scientist, Dr. Barry Marshall wanted to prove bacteria caused ulcers and not stress.? No one believed him.? So he took the organisms from a patient, mixed it in a broth and drank it. He then biopsied himself and treated it. There's a non-uniform method that saved a lot of suffering.? Bravo we crazy scientists.? Anne -----Original Message----- From: Geoff via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 12:31 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain I cannot believe any scientist would advocate such a non-uniform method as spitting on a slide. Buy a bottle of what ever enzyme and use a reproducible buffer and temperature. Geoff On 5/5/2016 3:19 PM, Anne Murvosh via Histonet wrote: > Yes, spitting is the tried and true way to do it.? Not to mention no measuring and cheaper.? The reason we switched to a powder is because I just don't spit well I used to have someone do it for me cause I would end up drooling. YUCK! The best way to find out is do the amylase method and the spit method at the same time and have the doctor pick the best.? A fun experiment? Anne > > -----Original Message----- > From: Bob Richmond via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, May 05, 2016 11:36 AM > To: koellingr at comcast.net > Cc: Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] PAS Stain > > Spokane Ray points out something I've wondered about for years - can > just anybody spit on the slide and remove the glycogen? I've never > heard of any variation, but the number of people I've asked is very > limited. This > reference: > http://www.ncbi.nlm.nih.gov/gene/276 > certainly suggests that different people have different salivary alpha > amylase activity. > > Bob Richmond > > On Thu, May 5, 2016 at 2:27 PM, wrote: > >> I love having the Samuri Pathologist on this forum for wisdom and >> real-laboratory life knowledge.? And yes, I have in the past spit on >> slide ON OCCASSION when faced with a dire necessity.? Although I know >> there are those who would wretch about this; it remains a fact of >> viable laboratory life for some. >> >> My problem now is that in this era of (MUCH TOO MUCH) regulation, how >> do you "test lots" or control from "lot-to-lot variation" in this >> SOP?? When Jane or Joe do this routinely and then goes on vacation, >> what about Sally or Jim spit?? There is a variation in copy number of >> the AMY1 gene >> (amylase) and resulting difference in amylase protein concentration >> amongst individuals. >> >> Why not just standardize it from the start, reagent, pH, temperature >> and it really cannot fail. >> >> Spokane Ray >> >> ------------------------------ >> *From: *"Bob Richmond via Histonet" >> >> *To: *"Histonet at lists.utsouthwestern.edu" < >> histonet at lists.utsouthwestern.edu> >> *Sent: *Thursday, May 5, 2016 11:10:40 AM >> *Subject: *Re: [Histonet] PAS Stain >> >> >> Amylase (diastase) for the PAS stain queries: >> >> Whatever happened to spitting on the slide (30 min at room temperature)? >> John Kiernan advises "thinking of lemons and drooling into a small beaker" >> though I'd advise chewing on a rubber band for a few seconds. >> >> He notes that alpha amylase is preferred. I'd go with the cheapest >> one in the Sigma-Aldrich catalog. Room temperature is usual, but I >> note that Sigma offers a heat-stable alpha amylase. >> >> Bob Richmond >> Samurai Pathologist >> Maryville TN >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff at rwjms.rutgers.edu ********************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ?? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Fri May 6 08:25:17 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 6 May 2016 13:25:17 +0000 (UTC) Subject: [Histonet] PAS Stain In-Reply-To: References: Message-ID: <2072960125.334350.1462541117817.JavaMail.yahoo@mail.yahoo.com> As I see it, there are 3 main objections about using human saliva as an amylase source.In order of importance they are:1- you will never know the actual concentration of the amylase and this will produce reproducibility problems.2- along with the saliva?you will introduce bacteria that may end being stained and can cause misdiagnoses.3- it is absolutely disgusting.Ren? On Thursday, May 5, 2016 2:26 PM, Bob Richmond via Histonet wrote: Amylase (diastase) for the PAS stain queries: Whatever happened to spitting on the slide (30 min at room temperature)? John Kiernan advises "thinking of lemons and drooling into a small beaker" though I'd advise chewing on a rubber band for a few seconds. He notes that alpha amylase is preferred. I'd go with the cheapest one in the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma offers a heat-stable alpha amylase. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Fri May 6 08:30:03 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 6 May 2016 13:30:03 +0000 (UTC) Subject: [Histonet] picric acid In-Reply-To: References: Message-ID: <904447824.366493.1462541403067.JavaMail.yahoo@mail.yahoo.com> Picric acid is an expensive reagent useful in many histology procedures.The advise you received of adding water is a good one.Humid picric acid will not explode at all. Why waste a good reagent?Keep humid, you will eventually used it.Ren? On Thursday, May 5, 2016 3:24 PM, Mca Werdler via Histonet wrote: Dear histonetters, Since a few months, i started working in a histology lab, run only by me ( coworkers are not specialized in histology). There has not worked here a person at histology for about 2 years. After many new protocols, i decided to clear out some chemicals. Now i found around 1 KG of DRY picric acid. I informed my coworkers about this, and they said just to dissolve everything in water. What do you guys think is the best way for handeling with this explosive chemical? Thank you all in advance! Maarten _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.benavides at eae.csic.es Fri May 6 09:11:01 2016 From: j.benavides at eae.csic.es (Julio Benavides) Date: Fri, 6 May 2016 16:11:01 +0200 Subject: [Histonet] picric acid In-Reply-To: <904447824.366493.1462541403067.JavaMail.yahoo@mail.yahoo.com> References: <904447824.366493.1462541403067.JavaMail.yahoo@mail.yahoo.com> Message-ID: Hi, For how long can you keep it in water? any particular dilution or just keep it humid (saturation)? We also do have some dry picric acid in the lab and, after reading about the bomb squad, I was begining to get concerned... Thanks a lot julio El 06/05/2016 a las 15:30, Rene J Buesa via Histonet escribi?: > Picric acid is an expensive reagent useful in many histology procedures.The advise you received of adding water is a good one.Humid picric acid will not explode at all. Why waste a good reagent?Keep humid, you will eventually used it.Ren? > > On Thursday, May 5, 2016 3:24 PM, Mca Werdler via Histonet wrote: > > > Dear histonetters, > > Since a few months, i started working in a histology lab, run only by me ( > coworkers are not specialized in histology). There has not worked here a > person at histology for about 2 years. > > After many new protocols, i decided to clear out some chemicals. > Now i found around 1 KG of DRY picric acid. I informed my coworkers about > this, and they said just to dissolve everything in water. > > What do you guys think is the best way for handeling with this explosive > chemical? Thank you all in advance! > > Maarten > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Fri May 6 09:41:38 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 6 May 2016 14:41:38 +0000 Subject: [Histonet] Slide Printer Message-ID: <48E053DDF6CE074DB6A7414BA05403F8071E80@HRHEX02-HOS.holyredeemer.local> We've used Leica for 8 years and everyone here loves it. We have it loaded with Leica brand plus slide, Leica regular clipped corner slides, and Thin Prep Slides from Cytologic. It was easily interfaced with CoPath, seldom jams, and we average about 1 unexpected service call per year in addition to the annual pm Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Message: 17 Date: Thu, 5 May 2016 18:45:39 -0400 From: Ginny Achstetter I need a recommendation on a slide printer. Tried the Leica and liked it but it only works well with their round edged slides and they aren't charged. Sent from my iPhone From Timothy.Morken at ucsf.edu Fri May 6 10:27:41 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 6 May 2016 15:27:41 +0000 Subject: [Histonet] picric acid In-Reply-To: References: <904447824.366493.1462541403067.JavaMail.yahoo@mail.yahoo.com> Message-ID: <761E2B5697F795489C8710BCC72141FF6FD4A89A@ex07.net.ucsf.edu> Julio, you can just pour water into the container. We always oversaturated so that a layer of water was on top of the powder. Look at this explanation http://oag.ca.gov/sites/all/files/agweb/pdfs/cci/safety/picric.pdf or read the text below if you cannot open this. This contains instructions on how to properly store picric acid powder, and how to deal with dry powder found in the lab. Long ago I had the pleasure of discovering a batch of six 2kg bottles of dry picric acid in our "bunker" where we stored flammables. Over 10 years old according to dates on the box. We called in the fire department to take care of it. They hosed it down, removed it and disposed of it; how, I don't know. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center +++++++++++++++ PICRIC ACID HAZARDS Mark Cameron, CIH Every couple months, an article appears in the local paper about a bomb disposal team removing picric acid that was found in a laboratory. The material is usually taken to be blown up. So why is picric acid considered so dangerous? Well, let?s look at the history of the use of Picric Acid and see what can be done to avoid those types of situations. Picric Acid (2,4,6 Trinitrophenol) is frequently found in forensic laboratories for use in the Christmas Tree stain (1) and for Urine detection (2). Histology uses include connective tissue stain (Jullien?s picroindogocarmine and Van Gieson?s picro-acid fuchsin), cytoplasmic stain (Van Gieson?s with iron hematoxylin), woody sections (picro aniline blue) and as a fixative agent (3). It was used in medicinal formulations in the treatment of malaria, trichinosis, herpes, smallpox and antiseptics. A one- percent solution was also used in the treatment of burns (4). British Chemist Peter Woulfe discovered picric acid in 1771. Picric acid was named from the Greek word pikros, which means ?bitter? due to its bitter taste (5). It was used to dye silk and wool yellow. Workers making picric acid during World War I were called ?canaries? because their skin was stained yellow (6). The explosive characteristics of Picric acid were discovered early. In 1885, experiments with picric acid were conducted in Lydd, England and the English adopted it as an explosive material called Lyddite in 1888. It was used extensively in bombs and grenades during World War I (7). Anhydrous Picric acid is similar to TNT. It needs usually needs a ?booster? such as a primer to create the explosion. However, as a strong acid, picric acid attacks common metals (except tin and aluminum) creating explosive salts, which are shock-sensitive. Bombs, mines and grenades were coated with tin or ashpatim to prevent the picric acid from contacting the metallic shell (8). Several catastrophic events involving picric acid have occurred. On December 6, 1917, an ammunition ship in Nova Scotia carrying 2,300 tons of picric acid as well as 400,000 pounds of TNT caught fire and exploded. Over 1,900 people were killed immediately and 9,000 were injured (9). Shock-sensitive metal picrates demonstrated their hazardous nature on May 1, 1916 when a fire at a French ammunition factory caused molten picric acid to flow onto the concrete floor. Calcium picrate was formed and detonated, killing 170 people (10). 06/18/02 Have there been any explosions in laboratories? There are no documented instances of spontaneous detonation of picric acid in a laboratory (11). The Department of Transportation classifies Picric Acid (Trinitrophenol) with less than 30% water by mass as a Class 1.1D explosive; with greater than 10% water by volume, it is a class 4.1 flammable solid (12). In the wetted state, it is unlikely to be an explosive hazard. If a bomb squad tries to blow it up, the picric acid will not detonate (13) and will just spread picric all over the area! The big concern has been with finding dehydrated picric acid. The most dangerous situations is if the bottle is old and has a metal cap. Under these circumstances, shock sensitive metal picrates may have formed on the cap contact area. Explosive experts should be contacted under these situations. Knowledgeable bomb disposal experts will use a robot to pick up the container and place it in water to re-hydrate the material (14) or remove it for detonation elsewhere. If a plastic cap is present, and the acid inside has dried, some crystals may be on the threads and the friction of removing a plastic cap might be enough to detonate the container. Under these circumstances, the container may be safe enough to place in a pail of water. Submerge the bottle to allow water to enter the cap and threads and dissolve any crystals that might be on the threads. Add ice to cause shrinkage of the bottle to enhance penetration of the water. Leave it like this for several days, until water can be seen inside the bottle. At this point, it is safe to open the cap and re-hydrate the acid inside (15). Whenever in doubt, contact explosives experts. Of course, an ounce of prevention is worth a pound of cure. If you really need to have picric acid in your lab, here?s what you should do: 1. Make sure that the picric acid is kept wet! Do not open a new bottle until needed. Then date the container to show when it was first used to help you in a routine inspection program. As part of your lab inspection program, check the hydration of your picric acid at least every six months and add distilled water as necessary. 2. Do not use metal spatulas to remove the material. 3. Be sure to clean the bottleneck, cap and threads with a wet cloth before resealing (16). 4. Get rid of old bottles with metal caps 5. Do not store large amounts of picric acid. Dispose of your picric acid every two years (17). 6. If possible, eliminate it from your inventory by purchasing premixed stains or a 1% solution for using in stain preparation. If you decide to dispose of your wet picric acid, several options are available. First, you could try reducing the picric acid to a non-explosive form using sodium hydroxide and sodium sulfide (18). After this treatment, the material will still be toxic and have to be disposed of as hazardous waste. Alternatively, it could be manifested as a flammable solid for hazardous waste and disposed of by incineration. DO NOT pour it down the drain; it could react with copper or iron piping to form the explosive salts. As a last consideration, Picric Acid is toxic. Ingestion of 1-2 grams would cause severe poisoning. The dust is irritating to the skin and eye. A peculiar effect on the eye is ?yellow? tainted vision. Systemic poisoning causes headache, vertigo, nausea, vomiting and diarrhea. The skin will turn yellow in severe exposures. Red colored urine may be produced (19). These symptoms would not expected in the laboratory environment under traditional uses. REFERENCES 1. Gaensslen, R., Mertens, J., Lee, H., Stolotow, M., ?Staining and Extraction Techniques?, Proceeding of a Forensic Science Symposium on the Analysis of Sexual Assault Evidence., FBI Academy, 1983. 2. Slot C. ?Plasma creatinine determination. A new and specific Jaffe reaction method.? Scand J. Clin. Lab. Invest. 1965, 17: 381 3. Lillie, R.D., ?H.J. Conn?s Biological Stains?, Williams & Wilkins Company, 1969, Baltimore, MD, pages 5, 60-61. 4. Patty?s Toxicology, John Wiley & Sons: New York, 2000, Volume IIB, page 980. 5. Davis, Tenney, ?The Chemistry of Powder and Explosives?, Angriff Press, 1984, page 164. 6. Hamilton, Alice, ?Exploring the Dangerous Trades?, American Industrial Hygiene Association: Fairfax, Virginia, 1995, page 185. 7. Cooper, Paul, ?Explosives Engineering?, Wiley-VCH, 1996, page 33. 8. Davis, ibid. 9. Phifer, Russell, ?Picric Acid: When is Panic Justified??, Speaking of Safety, Volume 9, No. 2, 2000, page 1-3. 10. Medard, Louis, ?Accidental Explosions, Volume 2: Types of Explosive Substances?, John Wiley and Sons: New York, 1989, page 739. 11. Phifer, ibid. 12. Code of Federal Regulations, Title 49, Section 172.101. 13. Kraut, Irv, In Handbook of Chemical Health and Safety, Alaimo, Robert J., Ed., Oxford University Press; New York, 2001, page 406. 14. Personal Communication with Tom Gundlach of RHR Inc., August 23, 2000. 15. Guidance for the Management of Reactive Chemicals, Picric Acid, http://www.uwsa.edu/oslp/ehs/info/picric.htm , 8/97 revision. 16. Safe Use and Management of Picric Acid, Safety Net #104, http://wwwehs.ucdavis.edu/sflynet/sn-104.html ,11/21/01 17. Biological & Chemical Safety Code, Appendix H-3, Handling Procedures for Unstable Agents, University of Saskatchewan, Department of Health and Safety, http://duke.usask.ca/~whiterv/unstable.html, 11/21/01. 18. Lunn, George and Sansone, Eric B., ?Destruction of Hazardous Chemicals in the Laboratory?, John Wiley & Sons: New York, 1990, page 219-221. 06/18/02 19. Documentation of the Threshold Limit Values, American Conference of Governmental Industrial Hygienists, Inc.: Cincinnati, Ohio, 1991, page 1271. 06/18/02 +++++++++++++++++++++++++++++++++++++++ -----Original Message----- From: Julio Benavides via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, May 06, 2016 7:11 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] picric acid Hi, For how long can you keep it in water? any particular dilution or just keep it humid (saturation)? We also do have some dry picric acid in the lab and, after reading about the bomb squad, I was begining to get concerned... Thanks a lot julio El 06/05/2016 a las 15:30, Rene J Buesa via Histonet escribi?: > Picric acid is an expensive reagent useful in many histology > procedures.The advise you received of adding water is a good one.Humid > picric acid will not explode at all. Why waste a good reagent?Keep > humid, you will eventually used it.Ren? > > On Thursday, May 5, 2016 3:24 PM, Mca Werdler via Histonet wrote: > > > Dear histonetters, > > Since a few months, i started working in a histology lab, run only by > me ( coworkers are not specialized in histology). There has not worked > here a person at histology for about 2 years. > > After many new protocols, i decided to clear out some chemicals. > Now i found around 1 KG of DRY picric acid. I informed my coworkers > about this, and they said just to dissolve everything in water. > > What do you guys think is the best way for handeling with this > explosive chemical? Thank you all in advance! > > Maarten > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Fri May 6 10:37:34 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 6 May 2016 15:37:34 +0000 Subject: [Histonet] picric acid In-Reply-To: <761E2B5697F795489C8710BCC72141FF6FD4A89A@ex07.net.ucsf.edu> References: <904447824.366493.1462541403067.JavaMail.yahoo@mail.yahoo.com> <761E2B5697F795489C8710BCC72141FF6FD4A89A@ex07.net.ucsf.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF6FD4A8B0@ex07.net.ucsf.edu> Here's another good document on how to handle picric acid powder www.ehs.wisc.edu/chem/SafeHandlingOfPicricAcid.pdf -----Original Message----- From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, May 06, 2016 8:28 AM To: Julio Benavides Cc: Histonet Subject: Re: [Histonet] picric acid Julio, you can just pour water into the container. We always oversaturated so that a layer of water was on top of the powder. Look at this explanation http://oag.ca.gov/sites/all/files/agweb/pdfs/cci/safety/picric.pdf or read the text below if you cannot open this. This contains instructions on how to properly store picric acid powder, and how to deal with dry powder found in the lab. Long ago I had the pleasure of discovering a batch of six 2kg bottles of dry picric acid in our "bunker" where we stored flammables. Over 10 years old according to dates on the box. We called in the fire department to take care of it. They hosed it down, removed it and disposed of it; how, I don't know. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center +++++++++++++++ PICRIC ACID HAZARDS Mark Cameron, CIH Every couple months, an article appears in the local paper about a bomb disposal team removing picric acid that was found in a laboratory. The material is usually taken to be blown up. So why is picric acid considered so dangerous? Well, let?s look at the history of the use of Picric Acid and see what can be done to avoid those types of situations. Picric Acid (2,4,6 Trinitrophenol) is frequently found in forensic laboratories for use in the Christmas Tree stain (1) and for Urine detection (2). Histology uses include connective tissue stain (Jullien?s picroindogocarmine and Van Gieson?s picro-acid fuchsin), cytoplasmic stain (Van Gieson?s with iron hematoxylin), woody sections (picro aniline blue) and as a fixative agent (3). It was used in medicinal formulations in the treatment of malaria, trichinosis, herpes, smallpox and antiseptics. A one- percent solution was also used in the treatment of burns (4). British Chemist Peter Woulfe discovered picric acid in 1771. Picric acid was named from the Greek word pikros, which means ?bitter? due to its bitter taste (5). It was used to dye silk and wool yellow. Workers making picric acid during World War I were called ?canaries? because their skin was stained yellow (6). The explosive characteristics of Picric acid were discovered early. In 1885, experiments with picric acid were conducted in Lydd, England and the English adopted it as an explosive material called Lyddite in 1888. It was used extensively in bombs and grenades during World War I (7). Anhydrous Picric acid is similar to TNT. It needs usually needs a ?booster? such as a primer to create the explosion. However, as a strong acid, picric acid attacks common metals (except tin and aluminum) creating explosive salts, which are shock-sensitive. Bombs, mines and grenades were coated with tin or ashpatim to prevent the picric acid from contacting the metallic shell (8). Several catastrophic events involving picric acid have occurred. On December 6, 1917, an ammunition ship in Nova Scotia carrying 2,300 tons of picric acid as well as 400,000 pounds of TNT caught fire and exploded. Over 1,900 people were killed immediately and 9,000 were injured (9). Shock-sensitive metal picrates demonstrated their hazardous nature on May 1, 1916 when a fire at a French ammunition factory caused molten picric acid to flow onto the concrete floor. Calcium picrate was formed and detonated, killing 170 people (10). 06/18/02 Have there been any explosions in laboratories? There are no documented instances of spontaneous detonation of picric acid in a laboratory (11). The Department of Transportation classifies Picric Acid (Trinitrophenol) with less than 30% water by mass as a Class 1.1D explosive; with greater than 10% water by volume, it is a class 4.1 flammable solid (12). In the wetted state, it is unlikely to be an explosive hazard. If a bomb squad tries to blow it up, the picric acid will not detonate (13) and will just spread picric all over the area! The big concern has been with finding dehydrated picric acid. The most dangerous situations is if the bottle is old and has a metal cap. Under these circumstances, shock sensitive metal picrates may have formed on the cap contact area. Explosive experts should be contacted under these situations. Knowledgeable bomb disposal experts will use a robot to pick up the container and place it in water to re-hydrate the material (14) or remove it for detonation elsewhere. If a plastic cap is present, and the acid inside has dried, some crystals may be on the threads and the friction of removing a plastic cap might be enough to detonate the container. Under these circumstances, the container may be safe enough to place in a pail of water. Submerge the bottle to allow water to enter the cap and threads and dissolve any crystals that might be on the threads. Add ice to cause shrinkage of the bottle to enhance penetration of the water. Leave it like this for several days, until water can be seen inside the bottle. At this point, it is safe to open the cap and re-hydrate the acid inside (15). Whenever in doubt, contact explosives experts. Of course, an ounce of prevention is worth a pound of cure. If you really need to have picric acid in your lab, here?s what you should do: 1. Make sure that the picric acid is kept wet! Do not open a new bottle until needed. Then date the container to show when it was first used to help you in a routine inspection program. As part of your lab inspection program, check the hydration of your picric acid at least every six months and add distilled water as necessary. 2. Do not use metal spatulas to remove the material. 3. Be sure to clean the bottleneck, cap and threads with a wet cloth before resealing (16). 4. Get rid of old bottles with metal caps 5. Do not store large amounts of picric acid. Dispose of your picric acid every two years (17). 6. If possible, eliminate it from your inventory by purchasing premixed stains or a 1% solution for using in stain preparation. If you decide to dispose of your wet picric acid, several options are available. First, you could try reducing the picric acid to a non-explosive form using sodium hydroxide and sodium sulfide (18). After this treatment, the material will still be toxic and have to be disposed of as hazardous waste. Alternatively, it could be manifested as a flammable solid for hazardous waste and disposed of by incineration. DO NOT pour it down the drain; it could react with copper or iron piping to form the explosive salts. As a last consideration, Picric Acid is toxic. Ingestion of 1-2 grams would cause severe poisoning. The dust is irritating to the skin and eye. A peculiar effect on the eye is ?yellow? tainted vision. Systemic poisoning causes headache, vertigo, nausea, vomiting and diarrhea. The skin will turn yellow in severe exposures. Red colored urine may be produced (19). These symptoms would not expected in the laboratory environment under traditional uses. REFERENCES 1. Gaensslen, R., Mertens, J., Lee, H., Stolotow, M., ?Staining and Extraction Techniques?, Proceeding of a Forensic Science Symposium on the Analysis of Sexual Assault Evidence., FBI Academy, 1983. 2. Slot C. ?Plasma creatinine determination. A new and specific Jaffe reaction method.? Scand J. Clin. Lab. Invest. 1965, 17: 381 3. Lillie, R.D., ?H.J. Conn?s Biological Stains?, Williams & Wilkins Company, 1969, Baltimore, MD, pages 5, 60-61. 4. Patty?s Toxicology, John Wiley & Sons: New York, 2000, Volume IIB, page 980. 5. Davis, Tenney, ?The Chemistry of Powder and Explosives?, Angriff Press, 1984, page 164. 6. Hamilton, Alice, ?Exploring the Dangerous Trades?, American Industrial Hygiene Association: Fairfax, Virginia, 1995, page 185. 7. Cooper, Paul, ?Explosives Engineering?, Wiley-VCH, 1996, page 33. 8. Davis, ibid. 9. Phifer, Russell, ?Picric Acid: When is Panic Justified??, Speaking of Safety, Volume 9, No. 2, 2000, page 1-3. 10. Medard, Louis, ?Accidental Explosions, Volume 2: Types of Explosive Substances?, John Wiley and Sons: New York, 1989, page 739. 11. Phifer, ibid. 12. Code of Federal Regulations, Title 49, Section 172.101. 13. Kraut, Irv, In Handbook of Chemical Health and Safety, Alaimo, Robert J., Ed., Oxford University Press; New York, 2001, page 406. 14. Personal Communication with Tom Gundlach of RHR Inc., August 23, 2000. 15. Guidance for the Management of Reactive Chemicals, Picric Acid, http://www.uwsa.edu/oslp/ehs/info/picric.htm , 8/97 revision. 16. Safe Use and Management of Picric Acid, Safety Net #104, http://wwwehs.ucdavis.edu/sflynet/sn-104.html ,11/21/01 17. Biological & Chemical Safety Code, Appendix H-3, Handling Procedures for Unstable Agents, University of Saskatchewan, Department of Health and Safety, http://duke.usask.ca/~whiterv/unstable.html, 11/21/01. 18. Lunn, George and Sansone, Eric B., ?Destruction of Hazardous Chemicals in the Laboratory?, John Wiley & Sons: New York, 1990, page 219-221. 06/18/02 19. Documentation of the Threshold Limit Values, American Conference of Governmental Industrial Hygienists, Inc.: Cincinnati, Ohio, 1991, page 1271. 06/18/02 +++++++++++++++++++++++++++++++++++++++ -----Original Message----- From: Julio Benavides via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, May 06, 2016 7:11 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] picric acid Hi, For how long can you keep it in water? any particular dilution or just keep it humid (saturation)? We also do have some dry picric acid in the lab and, after reading about the bomb squad, I was begining to get concerned... Thanks a lot julio El 06/05/2016 a las 15:30, Rene J Buesa via Histonet escribi?: > Picric acid is an expensive reagent useful in many histology > procedures.The advise you received of adding water is a good one.Humid > picric acid will not explode at all. Why waste a good reagent?Keep > humid, you will eventually used it.Ren? > > On Thursday, May 5, 2016 3:24 PM, Mca Werdler via Histonet wrote: > > > Dear histonetters, > > Since a few months, i started working in a histology lab, run only by > me ( coworkers are not specialized in histology). There has not worked > here a person at histology for about 2 years. > > After many new protocols, i decided to clear out some chemicals. > Now i found around 1 KG of DRY picric acid. I informed my coworkers > about this, and they said just to dissolve everything in water. > > What do you guys think is the best way for handeling with this > explosive chemical? Thank you all in advance! > > Maarten > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.benavides at eae.csic.es Fri May 6 11:01:34 2016 From: j.benavides at eae.csic.es (Julio Benavides) Date: Fri, 06 May 2016 18:01:34 +0200 Subject: [Histonet] picric acid In-Reply-To: <761E2B5697F795489C8710BCC72141FF6FD4A8B0@ex07.net.ucsf.edu> References: <904447824.366493.1462541403067.JavaMail.yahoo@mail.yahoo.com> <761E2B5697F795489C8710BCC72141FF6FD4A89A@ex07.net.ucsf.edu> <761E2B5697F795489C8710BCC72141FF6FD4A8B0@ex07.net.ucsf.edu> Message-ID: <20160506180134.Horde.7fck-lVkajVdPgthWv7H2Q1@webmail.csic.es> Thank you so much everybody for your help!! "Morken, Timothy" escribi?: > Here's another good document on how to handle picric acid powder > > www.ehs.wisc.edu/chem/SafeHandlingOfPicricAcid.pdf > > > > -----Original Message----- > From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Friday, May 06, 2016 8:28 AM > To: Julio Benavides > Cc: Histonet > Subject: Re: [Histonet] picric acid > > Julio, you can just pour water into the container. We always > oversaturated so that a layer of water was on top of the powder. > > Look at this explanation > http://oag.ca.gov/sites/all/files/agweb/pdfs/cci/safety/picric.pdf > > or read the text below if you cannot open this. This contains > instructions on how to properly store picric acid powder, and how to > deal with dry powder found in the lab. > > Long ago I had the pleasure of discovering a batch of six 2kg > bottles of dry picric acid in our "bunker" where we stored > flammables. Over 10 years old according to dates on the box. We > called in the fire department to take care of it. They hosed it > down, removed it and disposed of it; how, I don't know. > > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology UC San Francisco Medical Center > > > +++++++++++++++ > PICRIC ACID HAZARDS > Mark Cameron, CIH > Every couple months, an article appears in the local paper about a > bomb disposal team removing picric acid that was found in a > laboratory. The material is usually taken to be blown up. So why is > picric acid considered so dangerous? Well, let?s look at the history > of the use of Picric Acid and see what can be done to avoid those > types of situations. > > Picric Acid (2,4,6 Trinitrophenol) is frequently found in forensic > laboratories for use in the Christmas Tree stain (1) and for Urine > detection (2). Histology uses include connective tissue stain > (Jullien?s picroindogocarmine and Van Gieson?s picro-acid fuchsin), > cytoplasmic stain (Van Gieson?s with iron hematoxylin), woody > sections (picro aniline blue) and as a fixative agent (3). It was > used in medicinal formulations in the treatment of malaria, > trichinosis, herpes, smallpox and antiseptics. A one- percent > solution was also used in the treatment of burns (4). > British Chemist Peter Woulfe discovered picric acid in 1771. Picric > acid was named from the Greek word pikros, which means ?bitter? due > to its bitter taste (5). It was used to dye silk and wool yellow. > Workers making picric acid during World War I were called ?canaries? > because their skin was stained yellow (6). > > The explosive characteristics of Picric acid were discovered early. > In 1885, experiments with picric acid were conducted in Lydd, > England and the English adopted it as an explosive material called > Lyddite in 1888. It was used extensively in bombs and grenades > during World War I (7). Anhydrous Picric acid is similar to TNT. It > needs usually needs a ?booster? such as a primer to create the > explosion. However, as a strong acid, picric acid attacks common > metals (except tin and aluminum) creating explosive salts, which are > shock-sensitive. Bombs, mines and grenades were coated with tin or > ashpatim to prevent the picric acid from contacting the metallic > shell (8). > > Several catastrophic events involving picric acid have occurred. On > December 6, 1917, an ammunition ship in Nova Scotia carrying 2,300 > tons of picric acid as well as 400,000 pounds of TNT caught fire and > exploded. Over 1,900 people were killed immediately and 9,000 were > injured (9). Shock-sensitive metal picrates demonstrated their > hazardous nature on May 1, 1916 when a fire at a French ammunition > factory caused molten picric acid to flow onto the concrete floor. > Calcium picrate was formed and detonated, killing 170 people (10). > 06/18/02 > > Have there been any explosions in laboratories? There are no > documented instances of spontaneous detonation of picric acid in a > laboratory (11). The Department of Transportation classifies Picric > Acid (Trinitrophenol) with less than 30% water by mass as a Class > 1.1D explosive; with greater than 10% water by volume, it is a class > 4.1 flammable solid (12). In the wetted state, it is unlikely to be > an explosive hazard. If a bomb squad tries to blow it up, the picric > acid will not detonate (13) and will just spread picric all over the > area! > The big concern has been with finding dehydrated picric acid. The > most dangerous situations is if the bottle is old and has a metal > cap. Under these circumstances, shock sensitive metal picrates may > have formed on the cap contact area. Explosive experts should be > contacted under these situations. Knowledgeable bomb disposal > experts will use a robot to pick up the container and place it in > water to re-hydrate the material (14) or remove it for detonation > elsewhere. > > If a plastic cap is present, and the acid inside has dried, some > crystals may be on the threads and the friction of removing a > plastic cap might be enough to detonate the container. Under these > circumstances, the container may be safe enough to place in a pail > of water. Submerge the bottle to allow water to enter the cap and > threads and dissolve any crystals that might be on the threads. Add > ice to cause shrinkage of the bottle to enhance penetration of the > water. Leave it like this for several days, until water can be seen > inside the bottle. At this point, it is safe to open the cap and > re-hydrate the acid inside (15). Whenever in doubt, contact > explosives experts. > Of course, an ounce of prevention is worth a pound of cure. If you > really need to have picric acid in your lab, here?s what you should > do: > 1. > Make sure that the picric acid is kept wet! Do not open a new bottle > until needed. Then date the container to show when it was first used > to help you in a routine inspection program. As part of your lab > inspection program, check the hydration of your picric acid at least > every six months and add distilled water as necessary. > 2. > Do not use metal spatulas to remove the material. > 3. > Be sure to clean the bottleneck, cap and threads with a wet cloth > before resealing (16). > 4. > Get rid of old bottles with metal caps > 5. > Do not store large amounts of picric acid. Dispose of your picric > acid every two years (17). > 6. > If possible, eliminate it from your inventory by purchasing premixed > stains or a 1% solution for using in stain preparation. > If you decide to dispose of your wet picric acid, several options > are available. First, you could try reducing the picric acid to a > non-explosive form using sodium hydroxide and sodium sulfide (18). > After this treatment, the material will still be toxic and have to be > disposed of as hazardous waste. Alternatively, it could be > manifested as a flammable solid for hazardous waste and disposed of > by incineration. DO NOT pour it down the drain; it could react with > copper or iron piping to form the explosive salts. > As a last consideration, Picric Acid is toxic. Ingestion of 1-2 > grams would cause severe poisoning. The dust is irritating to the > skin and eye. A peculiar effect on the eye is ?yellow? tainted > vision. Systemic poisoning causes headache, vertigo, nausea, > vomiting and diarrhea. The skin will turn yellow in severe > exposures. Red colored urine may be produced (19). These symptoms > would not expected in the laboratory environment under traditional > uses. > > REFERENCES > 1. > Gaensslen, R., Mertens, J., Lee, H., Stolotow, M., ?Staining and > Extraction Techniques?, Proceeding of a Forensic Science Symposium > on the Analysis of Sexual Assault Evidence., FBI Academy, 1983. > 2. > Slot C. ?Plasma creatinine determination. A new and specific Jaffe > reaction method.? Scand J. Clin. Lab. Invest. 1965, 17: 381 > 3. > Lillie, R.D., ?H.J. Conn?s Biological Stains?, Williams & Wilkins > Company, 1969, Baltimore, MD, pages 5, 60-61. > 4. > Patty?s Toxicology, John Wiley & Sons: New York, 2000, Volume IIB, page 980. > 5. > Davis, Tenney, ?The Chemistry of Powder and Explosives?, Angriff > Press, 1984, page 164. > 6. > Hamilton, Alice, ?Exploring the Dangerous Trades?, American > Industrial Hygiene Association: Fairfax, Virginia, 1995, page 185. > 7. > Cooper, Paul, ?Explosives Engineering?, Wiley-VCH, 1996, page 33. > 8. > Davis, ibid. > 9. > Phifer, Russell, ?Picric Acid: When is Panic Justified??, Speaking > of Safety, Volume 9, No. 2, 2000, page 1-3. > 10. > Medard, Louis, ?Accidental Explosions, Volume 2: Types of Explosive > Substances?, John Wiley and Sons: New York, 1989, page 739. > 11. > Phifer, ibid. > 12. > Code of Federal Regulations, Title 49, Section 172.101. > 13. > Kraut, Irv, In Handbook of Chemical Health and Safety, Alaimo, > Robert J., Ed., Oxford University Press; New York, 2001, page 406. > 14. > Personal Communication with Tom Gundlach of RHR Inc., August 23, 2000. > 15. > Guidance for the Management of Reactive Chemicals, Picric Acid, > http://www.uwsa.edu/oslp/ehs/info/picric.htm , 8/97 revision. > 16. > Safe Use and Management of Picric Acid, Safety Net #104, > http://wwwehs.ucdavis.edu/sflynet/sn-104.html ,11/21/01 > 17. > Biological & Chemical Safety Code, Appendix H-3, Handling Procedures > for Unstable Agents, University of Saskatchewan, Department of > Health and Safety, http://duke.usask.ca/~whiterv/unstable.html, > 11/21/01. > 18. > Lunn, George and Sansone, Eric B., ?Destruction of Hazardous > Chemicals in the Laboratory?, John Wiley & Sons: New York, 1990, > page 219-221. > 06/18/02 > 19. > Documentation of the Threshold Limit Values, American Conference of > Governmental Industrial Hygienists, Inc.: Cincinnati, Ohio, 1991, > page 1271. > 06/18/02 > > > +++++++++++++++++++++++++++++++++++++++ > -----Original Message----- > From: Julio Benavides via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Friday, May 06, 2016 7:11 AM > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] picric acid > > Hi, > > For how long can you keep it in water? any particular dilution or > just keep it humid (saturation)? > > We also do have some dry picric acid in the lab and, after reading > about the bomb squad, I was begining to get concerned... > > Thanks a lot > > julio > > > El 06/05/2016 a las 15:30, Rene J Buesa via Histonet escribi?: >> Picric acid is an expensive reagent useful in many histology >> procedures.The advise you received of adding water is a good one.Humid >> picric acid will not explode at all. Why waste a good reagent?Keep >> humid, you will eventually used it.Ren? >> >> On Thursday, May 5, 2016 3:24 PM, Mca Werdler via Histonet >> wrote: >> >> >> Dear histonetters, >> >> Since a few months, i started working in a histology lab, run only by >> me ( coworkers are not specialized in histology). There has not worked >> here a person at histology for about 2 years. >> >> After many new protocols, i decided to clear out some chemicals. >> Now i found around 1 KG of DRY picric acid. I informed my coworkers >> about this, and they said just to dissolve everything in water. >> >> What do you guys think is the best way for handeling with this >> explosive chemical? Thank you all in advance! >> >> Maarten >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abadesuyi at nrh-ok.com Fri May 6 12:08:19 2016 From: abadesuyi at nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Fri, 6 May 2016 12:08:19 -0500 Subject: [Histonet] Looking for Part Time Position in Oklahoma Message-ID: <04EE4F75BB5FB246ADB68D69B7460443A5177B10DC@MAIL.nrhnt.nrh-ok.com> Hi, I am looking for a part time histo position in Oklahoma City, OK. Thanks, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Cell: 405-973-6363 Tel: 405- 307- 1145 abadesuyi at nrh-ok.com ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From edmartin26 at gmail.com Fri May 6 13:58:47 2016 From: edmartin26 at gmail.com (Eddie Martin) Date: Fri, 6 May 2016 13:58:47 -0500 Subject: [Histonet] Automated IHC instrument Message-ID: <069D742F-813A-4A3B-8468-5DDD6B451D1D@gmail.com> Hi Valerie, Any platform would get the job done well. They all offer reagent rental agreements too. Dako and Leica would require the user to be a bit more knowledgeable of IHC protocols than starting a run on a Ventana analyzer. Dako is semi-automated, whereas leica and ventana both have onboard retrieval, and reagent mixing of chromagen. In terms of the most open system, Dako would be the best analyzer followed by Leica in second place. Ventana does allow 3rd party antibodies to be run on it, but charge you a premium for open containers to run on their analyzer. In terms of antibodies: this may be better chosen by your pathologist which may have an impact on which analyzer you will also get, but I can suggest to you my favorites. Her2neu - all three vendors provide a good Her2neu clone. Dako?s Her2 sometimes doesn?t stain as well, so would be my least favorite of the 3. Ventana?s Her2 is the most widely used. ER - all 3 vendors are also good. PR - Dako and Novocastra have great PR clones. Ventana?s PR clone is IVD as Dako and Novocastra Clone but is not ASCO-CAP approved antibody. Ventana will push to say it is IVD, as Dako, and Novocastra (Leica), which is necessary for billing purposes, but their PR clone isn?t as effective as the other two i mentioned. Ki67 - Dako?s MIB-1 clone is like the gold standard for Ki-67. All three vendor versions work as intended. Leica removed one of their two clone options last year, so k2 clone for Leica is your only option. Ventana?s clone looks nicer in my opinion. Hope this helps somehow. Kind Regards, Eddie Martin, HT(ASCP), QIHC, HTL 954-826-9403 Edmartin26 at gmail.com From gachstetter at yahoo.com Fri May 6 17:57:15 2016 From: gachstetter at yahoo.com (Ginny Achstetter) Date: Fri, 6 May 2016 18:57:15 -0400 Subject: [Histonet] Leica printer Message-ID: <90D0CF47-7BDF-4228-B3A9-42671784CD26@yahoo.com> The Leica printer is a high output machine. Seems like the slide mate is more of a personal printer to go next to every microtome. I also found out that the Leica slides are charged. They have an x on the bottom of the slide. Fisher also makes the rounded edge slides that can be used on that machine. Sent from my iPhone From Megan.Dishop at childrensmn.org Fri May 6 17:59:06 2016 From: Megan.Dishop at childrensmn.org (Megan Dishop) Date: Fri, 6 May 2016 17:59:06 -0500 Subject: [Histonet] Histology supervisor position - Children's Minnesota Message-ID: <572CDB6A.D202.00E3.1@childrensmn.org> We are looking for a team lead for the histology and immunohistochemistry laboratory at Children's Hospitals and Clinics of Minnesota in Minneapolis-St. Paul. This is a great opportunity in a growing pediatric healthcare network! Please apply using the link below. http://childrensmn.taleo.net/careersection/ex/jobdetail.ftl?job=1602705I Megan K. Dishop MD Medical Director, Pediatric Anatomic Pathology Children's Hospitals and Clinics of Minnesota Laboratories 2525 Chicago Ave S. MS32-B600, Minneapolis, MN 55404 USA Phone: 612-813-6521 Fax: 612-813-7721 Email: megan.dishop at childrensMN.org Adjoint Professor of Pediatrics, University of Colorado School of Medicine Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. From ewj at pigsqq.org Fri May 6 21:13:10 2016 From: ewj at pigsqq.org (E. Wayne Johnson) Date: Sat, 7 May 2016 10:13:10 +0800 Subject: [Histonet] picric acid In-Reply-To: <20160506180134.Horde.7fck-lVkajVdPgthWv7H2Q1@webmail.csic.es> References: <904447824.366493.1462541403067.JavaMail.yahoo@mail.yahoo.com> <761E2B5697F795489C8710BCC72141FF6FD4A89A@ex07.net.ucsf.edu> <761E2B5697F795489C8710BCC72141FF6FD4A8B0@ex07.net.ucsf.edu> <20160506180134.Horde.7fck-lVkajVdPgthWv7H2Q1@webmail.csic.es> Message-ID: <67682c47-08a5-435d-5a1c-2b40c2c07e42@pigsqq.org> I read "Chemical Magic" and the "Anarchist's Handbook" in high school many years ago. That was back when boys carried pocket knives and sometime took their shotguns to school to show their friends. I still occasionally make a little bit of NI_3 for fun. We've tried to make picric acid explode and have taken it out in the yard and burned it and beat on it with hammers. It's really not all that easy to make it go "BOOM" or even make crisp pops. Just don't mix it with heavy metals. I agree strongly that it should be handled with respect, and with gloved hands like any staining powder. Store it wet, but the /~~paranoia~/~ about picric acid is exactly that. And on the part of the cops and bomb squads who take picric acid out and detonate it amid much drama and fanfare, heavy on the delusions and illusions of grandeur. E. Wayne Johnson, DVM Enable Ag-Tech Beijing On 05/07/2016 12:01 AM, Julio Benavides wrote: > Thank you so much everybody for your help!! > > > > "Morken, Timothy" escribi?: > >> Here's another good document on how to handle picric acid powder >> >> www.ehs.wisc.edu/chem/SafeHandlingOfPicricAcid.pdf >> >> >> >> -----Original Message----- >> From: Morken, Timothy via Histonet >> [mailto:histonet at lists.utsouthwestern.edu] >> Sent: Friday, May 06, 2016 8:28 AM >> To: Julio Benavides >> Cc: Histonet >> Subject: Re: [Histonet] picric acid >> >> Julio, you can just pour water into the container. We always >> oversaturated so that a layer of water was on top of the powder. >> >> Look at this explanation >> http://oag.ca.gov/sites/all/files/agweb/pdfs/cci/safety/picric.pdf >> >> or read the text below if you cannot open this. This contains >> instructions on how to properly store picric acid powder, and how to >> deal with dry powder found in the lab. >> >> Long ago I had the pleasure of discovering a batch of six 2kg bottles >> of dry picric acid in our "bunker" where we stored flammables. Over >> 10 years old according to dates on the box. We called in the fire >> department to take care of it. They hosed it down, removed it and >> disposed of it; how, I don't know. >> >> >> Tim Morken >> Pathology Site Manager, Parnassus >> Supervisor, Electron Microscopy/Neuromuscular Special Studies >> Department of Pathology UC San Francisco Medical Center >> >> >> +++++++++++++++ >> PICRIC ACID HAZARDS >> Mark Cameron, CIH >> Every couple months, an article appears in the local paper about a >> bomb disposal team removing picric acid that was found in a >> laboratory. The material is usually taken to be blown up. So why is >> picric acid considered so dangerous? Well, let?s look at the history >> of the use of Picric Acid and see what can be done to avoid those >> types of situations. >> >> Picric Acid (2,4,6 Trinitrophenol) is frequently found in forensic >> laboratories for use in the Christmas Tree stain (1) and for Urine >> detection (2). Histology uses include connective tissue stain >> (Jullien?s picroindogocarmine and Van Gieson?s picro-acid fuchsin), >> cytoplasmic stain (Van Gieson?s with iron hematoxylin), woody >> sections (picro aniline blue) and as a fixative agent (3). It was >> used in medicinal formulations in the treatment of malaria, >> trichinosis, herpes, smallpox and antiseptics. A one- percent >> solution was also used in the treatment of burns (4). >> British Chemist Peter Woulfe discovered picric acid in 1771. Picric >> acid was named from the Greek word pikros, which means ?bitter? due >> to its bitter taste (5). It was used to dye silk and wool yellow. >> Workers making picric acid during World War I were called ?canaries? >> because their skin was stained yellow (6). >> >> The explosive characteristics of Picric acid were discovered early. >> In 1885, experiments with picric acid were conducted in Lydd, England >> and the English adopted it as an explosive material called Lyddite in >> 1888. It was used extensively in bombs and grenades during World War >> I (7). Anhydrous Picric acid is similar to TNT. It needs usually >> needs a ?booster? such as a primer to create the explosion. However, >> as a strong acid, picric acid attacks common metals (except tin and >> aluminum) creating explosive salts, which are shock-sensitive. Bombs, >> mines and grenades were coated with tin or ashpatim to prevent the >> picric acid from contacting the metallic shell (8). >> >> Several catastrophic events involving picric acid have occurred. On >> December 6, 1917, an ammunition ship in Nova Scotia carrying 2,300 >> tons of picric acid as well as 400,000 pounds of TNT caught fire and >> exploded. Over 1,900 people were killed immediately and 9,000 were >> injured (9). Shock-sensitive metal picrates demonstrated their >> hazardous nature on May 1, 1916 when a fire at a French ammunition >> factory caused molten picric acid to flow onto the concrete floor. >> Calcium picrate was formed and detonated, killing 170 people (10). >> 06/18/02 >> >> Have there been any explosions in laboratories? There are no >> documented instances of spontaneous detonation of picric acid in a >> laboratory (11). The Department of Transportation classifies Picric >> Acid (Trinitrophenol) with less than 30% water by mass as a Class >> 1.1D explosive; with greater than 10% water by volume, it is a class >> 4.1 flammable solid (12). In the wetted state, it is unlikely to be >> an explosive hazard. If a bomb squad tries to blow it up, the picric >> acid will not detonate (13) and will just spread picric all over the >> area! >> The big concern has been with finding dehydrated picric acid. The >> most dangerous situations is if the bottle is old and has a metal >> cap. Under these circumstances, shock sensitive metal picrates may >> have formed on the cap contact area. Explosive experts should be >> contacted under these situations. Knowledgeable bomb disposal experts >> will use a robot to pick up the container and place it in water to >> re-hydrate the material (14) or remove it for detonation elsewhere. >> >> If a plastic cap is present, and the acid inside has dried, some >> crystals may be on the threads and the friction of removing a plastic >> cap might be enough to detonate the container. Under these >> circumstances, the container may be safe enough to place in a pail of >> water. Submerge the bottle to allow water to enter the cap and >> threads and dissolve any crystals that might be on the threads. Add >> ice to cause shrinkage of the bottle to enhance penetration of the >> water. Leave it like this for several days, until water can be seen >> inside the bottle. At this point, it is safe to open the cap and >> re-hydrate the acid inside (15). Whenever in doubt, contact >> explosives experts. >> Of course, an ounce of prevention is worth a pound of cure. If you >> really need to have picric acid in your lab, here?s what you should do: >> 1. >> Make sure that the picric acid is kept wet! Do not open a new bottle >> until needed. Then date the container to show when it was first used >> to help you in a routine inspection program. As part of your lab >> inspection program, check the hydration of your picric acid at least >> every six months and add distilled water as necessary. >> 2. >> Do not use metal spatulas to remove the material. >> 3. >> Be sure to clean the bottleneck, cap and threads with a wet cloth >> before resealing (16). >> 4. >> Get rid of old bottles with metal caps >> 5. >> Do not store large amounts of picric acid. Dispose of your picric >> acid every two years (17). >> 6. >> If possible, eliminate it from your inventory by purchasing premixed >> stains or a 1% solution for using in stain preparation. >> If you decide to dispose of your wet picric acid, several options are >> available. First, you could try reducing the picric acid to a >> non-explosive form using sodium hydroxide and sodium sulfide (18). >> After this treatment, the material will still be toxic and have to be >> disposed of as hazardous waste. Alternatively, it could be manifested >> as a flammable solid for hazardous waste and disposed of by >> incineration. DO NOT pour it down the drain; it could react with >> copper or iron piping to form the explosive salts. >> As a last consideration, Picric Acid is toxic. Ingestion of 1-2 grams >> would cause severe poisoning. The dust is irritating to the skin and >> eye. A peculiar effect on the eye is ?yellow? tainted vision. >> Systemic poisoning causes headache, vertigo, nausea, vomiting and >> diarrhea. The skin will turn yellow in severe exposures. Red colored >> urine may be produced (19). These symptoms would not expected in the >> laboratory environment under traditional uses. >> >> REFERENCES >> 1. >> Gaensslen, R., Mertens, J., Lee, H., Stolotow, M., ?Staining and >> Extraction Techniques?, Proceeding of a Forensic Science Symposium on >> the Analysis of Sexual Assault Evidence., FBI Academy, 1983. >> 2. >> Slot C. ?Plasma creatinine determination. A new and specific Jaffe >> reaction method.? Scand J. Clin. Lab. Invest. 1965, 17: 381 >> 3. >> Lillie, R.D., ?H.J. Conn?s Biological Stains?, Williams & Wilkins >> Company, 1969, Baltimore, MD, pages 5, 60-61. >> 4. >> Patty?s Toxicology, John Wiley & Sons: New York, 2000, Volume IIB, >> page 980. >> 5. >> Davis, Tenney, ?The Chemistry of Powder and Explosives?, Angriff >> Press, 1984, page 164. >> 6. >> Hamilton, Alice, ?Exploring the Dangerous Trades?, American >> Industrial Hygiene Association: Fairfax, Virginia, 1995, page 185. >> 7. >> Cooper, Paul, ?Explosives Engineering?, Wiley-VCH, 1996, page 33. >> 8. >> Davis, ibid. >> 9. >> Phifer, Russell, ?Picric Acid: When is Panic Justified??, Speaking of >> Safety, Volume 9, No. 2, 2000, page 1-3. >> 10. >> Medard, Louis, ?Accidental Explosions, Volume 2: Types of Explosive >> Substances?, John Wiley and Sons: New York, 1989, page 739. >> 11. >> Phifer, ibid. >> 12. >> Code of Federal Regulations, Title 49, Section 172.101. >> 13. >> Kraut, Irv, In Handbook of Chemical Health and Safety, Alaimo, Robert >> J., Ed., Oxford University Press; New York, 2001, page 406. >> 14. >> Personal Communication with Tom Gundlach of RHR Inc., August 23, 2000. >> 15. >> Guidance for the Management of Reactive Chemicals, Picric Acid, >> http://www.uwsa.edu/oslp/ehs/info/picric.htm , 8/97 revision. >> 16. >> Safe Use and Management of Picric Acid, Safety Net #104, >> http://wwwehs.ucdavis.edu/sflynet/sn-104.html ,11/21/01 >> 17. >> Biological & Chemical Safety Code, Appendix H-3, Handling Procedures >> for Unstable Agents, University of Saskatchewan, Department of Health >> and Safety, http://duke.usask.ca/~whiterv/unstable.html, 11/21/01. >> 18. >> Lunn, George and Sansone, Eric B., ?Destruction of Hazardous >> Chemicals in the Laboratory?, John Wiley & Sons: New York, 1990, page >> 219-221. >> 06/18/02 >> 19. >> Documentation of the Threshold Limit Values, American Conference of >> Governmental Industrial Hygienists, Inc.: Cincinnati, Ohio, 1991, >> page 1271. >> 06/18/02 >> >> >> +++++++++++++++++++++++++++++++++++++++ >> -----Original Message----- >> From: Julio Benavides via Histonet >> [mailto:histonet at lists.utsouthwestern.edu] >> Sent: Friday, May 06, 2016 7:11 AM >> To: histonet at lists.utsouthwestern.edu >> Subject: Re: [Histonet] picric acid >> >> Hi, >> >> For how long can you keep it in water? any particular dilution or >> just keep it humid (saturation)? >> >> We also do have some dry picric acid in the lab and, after reading >> about the bomb squad, I was begining to get concerned... >> >> Thanks a lot >> >> julio >> >> >> El 06/05/2016 a las 15:30, Rene J Buesa via Histonet escribi?: >>> Picric acid is an expensive reagent useful in many histology >>> procedures.The advise you received of adding water is a good one.Humid >>> picric acid will not explode at all. Why waste a good reagent?Keep >>> humid, you will eventually used it.Ren? >>> >>> On Thursday, May 5, 2016 3:24 PM, Mca Werdler via Histonet >>> wrote: >>> >>> >>> Dear histonetters, >>> >>> Since a few months, i started working in a histology lab, run only by >>> me ( coworkers are not specialized in histology). There has not worked >>> here a person at histology for about 2 years. >>> >>> After many new protocols, i decided to clear out some chemicals. >>> Now i found around 1 KG of DRY picric acid. I informed my coworkers >>> about this, and they said just to dissolve everything in water. >>> >>> What do you guys think is the best way for handeling with this >>> explosive chemical? Thank you all in advance! >>> >>> Maarten >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > From mprice63 at live.com Sat May 7 15:48:46 2016 From: mprice63 at live.com (Marsha Price) Date: Sat, 7 May 2016 20:48:46 +0000 Subject: [Histonet] Histonet Digest, Vol 150, Issue 10 In-Reply-To: References: Message-ID: I know this subject has been discussed on here before, but can someone direct me to the correct link for the application to get your Florida HT application? Thank You. Marsha Price Sent from my iPhone > On May 7, 2016, at 12:00 PM, wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Looking for Part Time Position in Oklahoma > (Adesupo, Adesuyi (Banjo)) > 2. Re: Automated IHC instrument (Eddie Martin) > 3. Leica printer (Ginny Achstetter) > 4. Histology supervisor position - Children's Minnesota > (Megan Dishop) > 5. Re: picric acid (E. Wayne Johnson) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 6 May 2016 12:08:19 -0500 > From: "Adesupo, Adesuyi (Banjo)" > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: [Histonet] Looking for Part Time Position in Oklahoma > Message-ID: > <04EE4F75BB5FB246ADB68D69B7460443A5177B10DC at MAIL.nrhnt.nrh-ok.com> > Content-Type: text/plain; charset="us-ascii" > > > Hi, > I am looking for a part time histo position in Oklahoma City, OK. > > Thanks, > > > Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS > > Histology Supervisor > > Norman Regional Health System, > > Norman, OK 73071. > > Cell: 405-973-6363 > > Tel: 405- 307- 1145 > abadesuyi at nrh-ok.com > > ====================================== > CONFIDENTIALITY NOTICE: > > This e-mail communication and any attachments may > contain confidential and privileged information for the use > of the designated recipients named above. If you are not > the intended recipient, you are hereby notified that you > have received this communication in error and that any > review, disclosure, dissemination, distribution, or copying > of it or its contents is prohibited. If you have received > this communication in error, please notify the sender > immediately and destroy all copies of this communication > and any attachments. > > > ------------------------------ > > Message: 2 > Date: Fri, 6 May 2016 13:58:47 -0500 > From: Eddie Martin > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Automated IHC instrument > Message-ID: <069D742F-813A-4A3B-8468-5DDD6B451D1D at gmail.com> > Content-Type: text/plain; charset=utf-8 > > Hi Valerie, > > Any platform would get the job done well. They all offer reagent rental agreements too. Dako and Leica would require the user to be a bit more knowledgeable of IHC protocols than starting a run on a Ventana analyzer. Dako is semi-automated, whereas leica and ventana both have onboard retrieval, and reagent mixing of chromagen. In terms of the most open system, Dako would be the best analyzer followed by Leica in second place. Ventana does allow 3rd party antibodies to be run on it, but charge you a premium for open containers to run on their analyzer. > > In terms of antibodies: this may be better chosen by your pathologist which may have an impact on which analyzer you will also get, but I can suggest to you my favorites. > > Her2neu - all three vendors provide a good Her2neu clone. Dako?s Her2 sometimes doesn?t stain as well, so would be my least favorite of the 3. Ventana?s Her2 is the most widely used. > > ER - all 3 vendors are also good. > > PR - Dako and Novocastra have great PR clones. Ventana?s PR clone is IVD as Dako and Novocastra Clone but is not ASCO-CAP approved antibody. Ventana will push to say it is IVD, as Dako, and Novocastra (Leica), which is necessary for billing purposes, but their PR clone isn?t as effective as the other two i mentioned. > > Ki67 - Dako?s MIB-1 clone is like the gold standard for Ki-67. All three vendor versions work as intended. Leica removed one of their two clone options last year, so k2 clone for Leica is your only option. Ventana?s clone looks nicer in my opinion. > > Hope this helps somehow. > > Kind Regards, > > Eddie Martin, HT(ASCP), QIHC, HTL > 954-826-9403 > Edmartin26 at gmail.com > > > ------------------------------ > > Message: 3 > Date: Fri, 6 May 2016 18:57:15 -0400 > From: Ginny Achstetter > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Leica printer > Message-ID: <90D0CF47-7BDF-4228-B3A9-42671784CD26 at yahoo.com> > Content-Type: text/plain; charset=us-ascii > > The Leica printer is a high output machine. Seems like the slide mate is more of a personal printer to go next to every microtome. I also found out that the Leica slides are charged. They have an x on the bottom of the slide. Fisher also makes the rounded edge slides that can be used on that machine. > > Sent from my iPhone > > > > ------------------------------ > > Message: 4 > Date: Fri, 6 May 2016 17:59:06 -0500 > From: "Megan Dishop" > To: histonet at lists.utsouthwestern.edu > Cc: "Jennifer Heimkes" , "Melissa > Turner" > Subject: [Histonet] Histology supervisor position - Children's > Minnesota > Message-ID: <572CDB6A.D202.00E3.1 at childrensmn.org> > Content-Type: text/plain; charset="utf-8" > > We are looking for a team lead for the histology and immunohistochemistry laboratory at Children's Hospitals and Clinics of Minnesota in Minneapolis-St. Paul. This is a great opportunity in a growing pediatric healthcare network! > > Please apply using the link below. > http://childrensmn.taleo.net/careersection/ex/jobdetail.ftl?job=1602705I > > > > Megan K. Dishop MD > Medical Director, Pediatric Anatomic Pathology > Children's Hospitals and Clinics of Minnesota Laboratories > 2525 Chicago Ave S. MS32-B600, Minneapolis, MN 55404 USA > Phone: 612-813-6521 Fax: 612-813-7721 Email: megan.dishop at childrensMN.org > Adjoint Professor of Pediatrics, University of Colorado School of Medicine > > Confidentiality Statement: > This email/fax, including attachments, may include confidential > and/or proprietary information and may be used only by the > person or entity to which it is addressed. If the reader of > this email/fax is not the intended recipient or his or her > agent, the reader is hereby notified that any dissemination, > distribution or copying of this email/fax is prohibited. If you > have received this email/fax in error, please notify the sender > by replying to this message and deleting this email or > destroying this facsimile immediately. > > ------------------------------ > > Message: 5 > Date: Sat, 7 May 2016 10:13:10 +0800 > From: "E. Wayne Johnson" > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] picric acid > Message-ID: <67682c47-08a5-435d-5a1c-2b40c2c07e42 at pigsqq.org> > Content-Type: text/plain; charset=windows-1252; format=flowed > > I read "Chemical Magic" and the "Anarchist's Handbook" in high school > many years ago. That was back when boys carried pocket knives and > sometime took their shotguns to school to show their friends. I still > occasionally make a little bit of NI_3 for fun. > > We've tried to make picric acid explode and have taken it out in the > yard and burned it and beat on it with hammers. It's really not all that > easy to make it go "BOOM" or even make crisp pops. Just don't mix it > with heavy metals. > > I agree strongly that it should be handled with respect, and with gloved > hands like any staining powder. Store it wet, but the /~~paranoia~/~ > about picric acid is exactly that. > > And on the part of the cops and bomb squads who take picric acid out and > detonate it amid much drama and fanfare, heavy on the delusions and > illusions of grandeur. > > E. Wayne Johnson, DVM > > Enable Ag-Tech > > Beijing > >> On 05/07/2016 12:01 AM, Julio Benavides wrote: >> Thank you so much everybody for your help!! >> >> >> >> "Morken, Timothy" escribi?: >> >>> Here's another good document on how to handle picric acid powder >>> >>> www.ehs.wisc.edu/chem/SafeHandlingOfPicricAcid.pdf >>> >>> >>> >>> -----Original Message----- >>> From: Morken, Timothy via Histonet >>> [mailto:histonet at lists.utsouthwestern.edu] >>> Sent: Friday, May 06, 2016 8:28 AM >>> To: Julio Benavides >>> Cc: Histonet >>> Subject: Re: [Histonet] picric acid >>> >>> Julio, you can just pour water into the container. We always >>> oversaturated so that a layer of water was on top of the powder. >>> >>> Look at this explanation >>> http://oag.ca.gov/sites/all/files/agweb/pdfs/cci/safety/picric.pdf >>> >>> or read the text below if you cannot open this. This contains >>> instructions on how to properly store picric acid powder, and how to >>> deal with dry powder found in the lab. >>> >>> Long ago I had the pleasure of discovering a batch of six 2kg bottles >>> of dry picric acid in our "bunker" where we stored flammables. Over >>> 10 years old according to dates on the box. We called in the fire >>> department to take care of it. They hosed it down, removed it and >>> disposed of it; how, I don't know. >>> >>> >>> Tim Morken >>> Pathology Site Manager, Parnassus >>> Supervisor, Electron Microscopy/Neuromuscular Special Studies >>> Department of Pathology UC San Francisco Medical Center >>> >>> >>> +++++++++++++++ >>> PICRIC ACID HAZARDS >>> Mark Cameron, CIH >>> Every couple months, an article appears in the local paper about a >>> bomb disposal team removing picric acid that was found in a >>> laboratory. The material is usually taken to be blown up. So why is >>> picric acid considered so dangerous? Well, let?s look at the history >>> of the use of Picric Acid and see what can be done to avoid those >>> types of situations. >>> >>> Picric Acid (2,4,6 Trinitrophenol) is frequently found in forensic >>> laboratories for use in the Christmas Tree stain (1) and for Urine >>> detection (2). Histology uses include connective tissue stain >>> (Jullien?s picroindogocarmine and Van Gieson?s picro-acid fuchsin), >>> cytoplasmic stain (Van Gieson?s with iron hematoxylin), woody >>> sections (picro aniline blue) and as a fixative agent (3). It was >>> used in medicinal formulations in the treatment of malaria, >>> trichinosis, herpes, smallpox and antiseptics. A one- percent >>> solution was also used in the treatment of burns (4). >>> British Chemist Peter Woulfe discovered picric acid in 1771. Picric >>> acid was named from the Greek word pikros, which means ?bitter? due >>> to its bitter taste (5). It was used to dye silk and wool yellow. >>> Workers making picric acid during World War I were called ?canaries? >>> because their skin was stained yellow (6). >>> >>> The explosive characteristics of Picric acid were discovered early. >>> In 1885, experiments with picric acid were conducted in Lydd, England >>> and the English adopted it as an explosive material called Lyddite in >>> 1888. It was used extensively in bombs and grenades during World War >>> I (7). Anhydrous Picric acid is similar to TNT. It needs usually >>> needs a ?booster? such as a primer to create the explosion. However, >>> as a strong acid, picric acid attacks common metals (except tin and >>> aluminum) creating explosive salts, which are shock-sensitive. Bombs, >>> mines and grenades were coated with tin or ashpatim to prevent the >>> picric acid from contacting the metallic shell (8). >>> >>> Several catastrophic events involving picric acid have occurred. On >>> December 6, 1917, an ammunition ship in Nova Scotia carrying 2,300 >>> tons of picric acid as well as 400,000 pounds of TNT caught fire and >>> exploded. Over 1,900 people were killed immediately and 9,000 were >>> injured (9). Shock-sensitive metal picrates demonstrated their >>> hazardous nature on May 1, 1916 when a fire at a French ammunition >>> factory caused molten picric acid to flow onto the concrete floor. >>> Calcium picrate was formed and detonated, killing 170 people (10). >>> 06/18/02 >>> >>> Have there been any explosions in laboratories? There are no >>> documented instances of spontaneous detonation of picric acid in a >>> laboratory (11). The Department of Transportation classifies Picric >>> Acid (Trinitrophenol) with less than 30% water by mass as a Class >>> 1.1D explosive; with greater than 10% water by volume, it is a class >>> 4.1 flammable solid (12). In the wetted state, it is unlikely to be >>> an explosive hazard. If a bomb squad tries to blow it up, the picric >>> acid will not detonate (13) and will just spread picric all over the >>> area! >>> The big concern has been with finding dehydrated picric acid. The >>> most dangerous situations is if the bottle is old and has a metal >>> cap. Under these circumstances, shock sensitive metal picrates may >>> have formed on the cap contact area. Explosive experts should be >>> contacted under these situations. Knowledgeable bomb disposal experts >>> will use a robot to pick up the container and place it in water to >>> re-hydrate the material (14) or remove it for detonation elsewhere. >>> >>> If a plastic cap is present, and the acid inside has dried, some >>> crystals may be on the threads and the friction of removing a plastic >>> cap might be enough to detonate the container. Under these >>> circumstances, the container may be safe enough to place in a pail of >>> water. Submerge the bottle to allow water to enter the cap and >>> threads and dissolve any crystals that might be on the threads. Add >>> ice to cause shrinkage of the bottle to enhance penetration of the >>> water. Leave it like this for several days, until water can be seen >>> inside the bottle. At this point, it is safe to open the cap and >>> re-hydrate the acid inside (15). Whenever in doubt, contact >>> explosives experts. >>> Of course, an ounce of prevention is worth a pound of cure. If you >>> really need to have picric acid in your lab, here?s what you should do: >>> 1. >>> Make sure that the picric acid is kept wet! Do not open a new bottle >>> until needed. Then date the container to show when it was first used >>> to help you in a routine inspection program. As part of your lab >>> inspection program, check the hydration of your picric acid at least >>> every six months and add distilled water as necessary. >>> 2. >>> Do not use metal spatulas to remove the material. >>> 3. >>> Be sure to clean the bottleneck, cap and threads with a wet cloth >>> before resealing (16). >>> 4. >>> Get rid of old bottles with metal caps >>> 5. >>> Do not store large amounts of picric acid. Dispose of your picric >>> acid every two years (17). >>> 6. >>> If possible, eliminate it from your inventory by purchasing premixed >>> stains or a 1% solution for using in stain preparation. >>> If you decide to dispose of your wet picric acid, several options are >>> available. First, you could try reducing the picric acid to a >>> non-explosive form using sodium hydroxide and sodium sulfide (18). >>> After this treatment, the material will still be toxic and have to be >>> disposed of as hazardous waste. Alternatively, it could be manifested >>> as a flammable solid for hazardous waste and disposed of by >>> incineration. DO NOT pour it down the drain; it could react with >>> copper or iron piping to form the explosive salts. >>> As a last consideration, Picric Acid is toxic. Ingestion of 1-2 grams >>> would cause severe poisoning. The dust is irritating to the skin and >>> eye. A peculiar effect on the eye is ?yellow? tainted vision. >>> Systemic poisoning causes headache, vertigo, nausea, vomiting and >>> diarrhea. The skin will turn yellow in severe exposures. Red colored >>> urine may be produced (19). These symptoms would not expected in the >>> laboratory environment under traditional uses. >>> >>> REFERENCES >>> 1. >>> Gaensslen, R., Mertens, J., Lee, H., Stolotow, M., ?Staining and >>> Extraction Techniques?, Proceeding of a Forensic Science Symposium on >>> the Analysis of Sexual Assault Evidence., FBI Academy, 1983. >>> 2. >>> Slot C. ?Plasma creatinine determination. A new and specific Jaffe >>> reaction method.? Scand J. Clin. Lab. Invest. 1965, 17: 381 >>> 3. >>> Lillie, R.D., ?H.J. Conn?s Biological Stains?, Williams & Wilkins >>> Company, 1969, Baltimore, MD, pages 5, 60-61. >>> 4. >>> Patty?s Toxicology, John Wiley & Sons: New York, 2000, Volume IIB, >>> page 980. >>> 5. >>> Davis, Tenney, ?The Chemistry of Powder and Explosives?, Angriff >>> Press, 1984, page 164. >>> 6. >>> Hamilton, Alice, ?Exploring the Dangerous Trades?, American >>> Industrial Hygiene Association: Fairfax, Virginia, 1995, page 185. >>> 7. >>> Cooper, Paul, ?Explosives Engineering?, Wiley-VCH, 1996, page 33. >>> 8. >>> Davis, ibid. >>> 9. >>> Phifer, Russell, ?Picric Acid: When is Panic Justified??, Speaking of >>> Safety, Volume 9, No. 2, 2000, page 1-3. >>> 10. >>> Medard, Louis, ?Accidental Explosions, Volume 2: Types of Explosive >>> Substances?, John Wiley and Sons: New York, 1989, page 739. >>> 11. >>> Phifer, ibid. >>> 12. >>> Code of Federal Regulations, Title 49, Section 172.101. >>> 13. >>> Kraut, Irv, In Handbook of Chemical Health and Safety, Alaimo, Robert >>> J., Ed., Oxford University Press; New York, 2001, page 406. >>> 14. >>> Personal Communication with Tom Gundlach of RHR Inc., August 23, 2000. >>> 15. >>> Guidance for the Management of Reactive Chemicals, Picric Acid, >>> http://www.uwsa.edu/oslp/ehs/info/picric.htm , 8/97 revision. >>> 16. >>> Safe Use and Management of Picric Acid, Safety Net #104, >>> http://wwwehs.ucdavis.edu/sflynet/sn-104.html ,11/21/01 >>> 17. >>> Biological & Chemical Safety Code, Appendix H-3, Handling Procedures >>> for Unstable Agents, University of Saskatchewan, Department of Health >>> and Safety, http://duke.usask.ca/~whiterv/unstable.html, 11/21/01. >>> 18. >>> Lunn, George and Sansone, Eric B., ?Destruction of Hazardous >>> Chemicals in the Laboratory?, John Wiley & Sons: New York, 1990, page >>> 219-221. >>> 06/18/02 >>> 19. >>> Documentation of the Threshold Limit Values, American Conference of >>> Governmental Industrial Hygienists, Inc.: Cincinnati, Ohio, 1991, >>> page 1271. >>> 06/18/02 >>> >>> >>> +++++++++++++++++++++++++++++++++++++++ >>> -----Original Message----- >>> From: Julio Benavides via Histonet >>> [mailto:histonet at lists.utsouthwestern.edu] >>> Sent: Friday, May 06, 2016 7:11 AM >>> To: histonet at lists.utsouthwestern.edu >>> Subject: Re: [Histonet] picric acid >>> >>> Hi, >>> >>> For how long can you keep it in water? any particular dilution or >>> just keep it humid (saturation)? >>> >>> We also do have some dry picric acid in the lab and, after reading >>> about the bomb squad, I was begining to get concerned... >>> >>> Thanks a lot >>> >>> julio >>> >>> >>> El 06/05/2016 a las 15:30, Rene J Buesa via Histonet escribi?: >>>> Picric acid is an expensive reagent useful in many histology >>>> procedures.The advise you received of adding water is a good one.Humid >>>> picric acid will not explode at all. Why waste a good reagent?Keep >>>> humid, you will eventually used it.Ren? >>>> >>>> On Thursday, May 5, 2016 3:24 PM, Mca Werdler via Histonet >>>> wrote: >>>> >>>> >>>> Dear histonetters, >>>> >>>> Since a few months, i started working in a histology lab, run only by >>>> me ( coworkers are not specialized in histology). There has not worked >>>> here a person at histology for about 2 years. >>>> >>>> After many new protocols, i decided to clear out some chemicals. >>>> Now i found around 1 KG of DRY picric acid. I informed my coworkers >>>> about this, and they said just to dissolve everything in water. >>>> >>>> What do you guys think is the best way for handeling with this >>>> explosive chemical? Thank you all in advance! >>>> >>>> Maarten >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet at lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>>> >>>> >>>> >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet at lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 150, Issue 10 > ***************************************** From mdpraet at gmail.com Sat May 7 17:51:32 2016 From: mdpraet at gmail.com (Mequita Praet) Date: Sat, 7 May 2016 18:51:32 -0400 Subject: [Histonet] Florida License application Message-ID: Here is the link to the Florida State License application. http://floridasclinicallabs.gov/licensing/ Mequita Praet From LaurenHegnerSweeney at uga.edu Mon May 9 07:47:44 2016 From: LaurenHegnerSweeney at uga.edu (Lauren Sweeney) Date: Mon, 9 May 2016 12:47:44 +0000 Subject: [Histonet] biogenex fast red Message-ID: Good Morning all in Histonet land, I was wondering if anyone has tried to make a smaller quantity of the fast red from Biogenex by breaking the tablet in half and using half the amount of diluent from the bottles in the kit? If so, how did you do this with the issues of the filter tip, etc. Thanks! Lauren From Lynne.Bell at cvmc.org Mon May 9 09:49:56 2016 From: Lynne.Bell at cvmc.org (Bell, Lynne) Date: Mon, 9 May 2016 14:49:56 +0000 Subject: [Histonet] Tracking surgical specimens brought to the lab Message-ID: For those of you who have specimens brought to your lab: do you have the person dropping off the specimen(s) initial a manifest to keep track of specimens actually dropped off. Case in point - a physician's office says they dropped off a specimen and we have no record of it being accessioned. I would like to track specimens dropped off and I am not sure how to accomplish this. I appreciate any feedback! Thanks, Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 (802)371-4923 From ihcworkshop at gmail.com Mon May 9 12:16:39 2016 From: ihcworkshop at gmail.com (Ihc Workshop) Date: Mon, 9 May 2016 10:16:39 -0700 Subject: [Histonet] IHC Wet Workshop announcement Message-ID: <3FAC1E78-7C1B-4CBC-9A99-FDA3F2BAF430@gmail.com> June 23 & 24, 2016. San Francisco Bay Area This IHC lab course is aimed at hands-on training of attendees in all aspects of IHC staining of human, mouse and animal tissues with varied antibodies. This is a small group workshop and is taught in a lab.....Lots of troubleshooting, only few spots left. To get more detail contact. Maria at: ihcworkshop at gmail.com From Heather.Seeley at tenethealth.com Mon May 9 12:35:02 2016 From: Heather.Seeley at tenethealth.com (Seeley, Heather) Date: Mon, 9 May 2016 17:35:02 +0000 Subject: [Histonet] PAS Stain In-Reply-To: References: Message-ID: <9DFE334E776E734D9D231EF60CCE93C57E26DB05@TENHDCTHMB10-31.tenethealth.net> We have one girl that always spits on our slide! :) works great! HEATHER SEELEY, HT(ASCP) Histotech 803-985-4676 OFFICE 803-327-7598 FAX ________________________________________ From: Bob Richmond [rsrichmond at gmail.com] Sent: Thursday, May 05, 2016 2:10 PM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain Amylase (diastase) for the PAS stain queries: Whatever happened to spitting on the slide (30 min at room temperature)? John Kiernan advises "thinking of lemons and drooling into a small beaker" though I'd advise chewing on a rubber band for a few seconds. He notes that alpha amylase is preferred. I'd go with the cheapest one in the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma offers a heat-stable alpha amylase. Bob Richmond Samurai Pathologist Maryville TN From jamiesonanderson at gmail.com Mon May 9 12:41:09 2016 From: jamiesonanderson at gmail.com (Jamieson Anderson) Date: Mon, 9 May 2016 17:41:09 +0000 (UTC) Subject: [Histonet] Tracking surgical specimens In-Reply-To: References: Message-ID: <537327FDD6BA9F6C.0B786CD3-7FD7-4574-B1DC-3D754EACB582@mail.outlook.com> Hi Lynne, In our lab we have a log book at Accessioning that each physician/nurse/porter/MOA signs when dropping off specimens. We also encourage them to drop off a QC sheet with a patient label for each specimen that is dropped off. We check these to ensure we have received each specimen, and we date/time stamp them and keep them on record in case we need to refer back to them in the future (ie. In case a clinician claims they dropped off a specimen we can prove they did not). Jamieson AndersonTechnical Coordinator - Anatomic PathologySt. Paul's HospitalLower Mainland Pathology & Laboratory Medicine _____________________________ Date: Mon, 9 May 2016 14:49:56 +0000 From: "Bell, Lynne" To: "Histonet (histonet at lists.utsouthwestern.edu)" Subject: [Histonet] Tracking surgical specimens brought to the lab Message-ID: Content-Type: text/plain; charset="us-ascii" For those of you who have specimens brought to your lab: do you have the person dropping off the specimen(s) initial a manifest to keep track of specimens actually dropped off. Case in point - a physician's office says they dropped off a specimen and we have no record of it being accessioned. I would like to track specimens dropped off and I am not sure how to accomplish this. I appreciate any feedback! Thanks, Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 (802)371-4923 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 150, Issue 12 ***************************************** From edmartin26 at gmail.com Mon May 9 12:51:46 2016 From: edmartin26 at gmail.com (Eddie Martin) Date: Mon, 9 May 2016 12:51:46 -0500 Subject: [Histonet] Tips on staining PLA2R assay on Leica Bond Message-ID: <16EDC8EC-EDAB-4F63-A3BD-BEC24EAB0BE3@gmail.com> I don?t do staining for this antibody, but have experience with IMF on frozen tissue and FFPE and have worked with the Leica BOND max and BOND-3. If you would prefer IHC over IMF, you can search for a human anti-mouse, or human anti-rabbit antibody. Abcam provides a polyclonal whole serum antibody that works on FFPE. I grabbed this from their website: Anti-PLA2R antibody (ab80054) Since Abcam?s PLA2R antibody is a rabbit anti-human, you would need to create a modified DAB or modified RED protocol that doesn?t include the secondary antibody / linker step in your protocol prior to dispensing polymer. The rest of your protocol can remain as it is. As you are using a Leica BOND, both ways are actually pretty easy to set up, either indirect IMF or using Leica?s DAB kit for IHC. You would just need to play around with retrieval times with both Citrate and EDTA to get the optimum staining pattern. Additional ancillaries may be added when necessary should you have any background stain that is hard to get out. Best Regards, Eddie Martin, HT(ASCP), QIHC 954-826-9403 Edmartin26 at gmail.com From victor_tobias at comcast.net Mon May 9 17:39:38 2016 From: victor_tobias at comcast.net (victor_tobias at comcast.net) Date: Mon, 9 May 2016 22:39:38 +0000 (UTC) Subject: [Histonet] PAS Stain In-Reply-To: <9DFE334E776E734D9D231EF60CCE93C57E26DB05@TENHDCTHMB10-31.tenethealth.net> References: <9DFE334E776E734D9D231EF60CCE93C57E26DB05@TENHDCTHMB10-31.tenethealth.net> Message-ID: <1237907839.22424188.1462833578398.JavaMail.zimbra@comcast.net> From criley at dpspa.com Tue May 10 04:47:18 2016 From: criley at dpspa.com (Charles Riley) Date: Tue, 10 May 2016 05:47:18 -0400 Subject: [Histonet] P16 Message-ID: Has anyone found a way to do p16 staining without purchasing anything from Ventana? My company wants to do P16 but refuses to by any ventana products and I have explained it is a waste of money to keep testing the antibodies from all the other companies. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From mullenmk at mail.magee.edu Tue May 10 09:54:55 2016 From: mullenmk at mail.magee.edu (Mullen, Mary) Date: Tue, 10 May 2016 14:54:55 +0000 Subject: [Histonet] Cytology/Histology Staining Question Message-ID: <374DC72E6B29D44086F8FF3289351B2508823897@MSXMBXNSPRD39.acct.upmchs.net> Hello all, I work in a small, low volume community hospital and was recently asked by a coworker why we do not just run both our cytology and histology slides on the same automated stainer (with their respective protocols). What I am wanting to know is if there is anyone currently running both staining protocols on a single automated stainer using common alcohols/xylenes/water? What are the pros/cons? Has there been any cross-contamination issues? We only run non-gyn cytology, all gyn cytology is sent out. Thanks, Mary K. Mullen, HTL(ASCP)CM Histotechnologist UPMC Northwest Seneca, PA From rjbuesa at yahoo.com Tue May 10 10:07:04 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 10 May 2016 15:07:04 +0000 (UTC) Subject: [Histonet] Cytology/Histology Staining Question In-Reply-To: <374DC72E6B29D44086F8FF3289351B2508823897@MSXMBXNSPRD39.acct.upmchs.net> References: <374DC72E6B29D44086F8FF3289351B2508823897@MSXMBXNSPRD39.acct.upmchs.net> Message-ID: <1252522947.1519943.1462892824143.JavaMail.yahoo@mail.yahoo.com> I would be concerned with potential cross-contamination. In my lab we had 2 staining instruments, one for cytology and other for histology.Ren? On Tuesday, May 10, 2016 10:59 AM, "Mullen, Mary via Histonet" wrote: Hello all, I work in a small, low volume community hospital and was recently asked by a coworker why we do not just run both our cytology and histology slides on the same automated stainer (with their respective protocols). What I am wanting to know is if there is anyone currently running both staining protocols on a single automated stainer using common alcohols/xylenes/water? What are the pros/cons? Has there been any cross-contamination issues? We only run non-gyn cytology, all gyn cytology is sent out. Thanks, Mary K. Mullen, HTL(ASCP)CM Histotechnologist UPMC Northwest Seneca, PA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa at alliedsearchpartners.com Tue May 10 10:44:21 2016 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Tue, 10 May 2016 15:44:21 +0000 Subject: [Histonet] ASCP Certified Pathologist Assistant Job Opening- Message-ID: Hello, I have a Full Time/Permanent job opening for an ASCP certified Pathologist Assistant in Ohio. Please contact me for details. Have a great day! Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 From Karen.Heckford at DignityHealth.org Tue May 10 10:44:33 2016 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Tue, 10 May 2016 08:44:33 -0700 Subject: [Histonet] Melanin Bleach Message-ID: Good Morning, I need some help. Yesterday I bleached some heavily pigmented tissue. I have to run some IHC's on them. I bought the bleaching kit from American Master Tech. I had to put the Permanganate for about 6 hours to get the melanin and then a couple of minutes in Oxalic Acid. I had to let them set overnight because I could not get another IHC run in that day. It looks like the tissue fell off during decloaking. I used Apex slides. I rarely ever have to bleach anything here. So I am not sure if I did this correctly. I am thinking I need to bleach and do the run in the same day and not let them set over night in DiH20. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From JRobinson at pathology-associates.com Tue May 10 11:33:26 2016 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Tue, 10 May 2016 16:33:26 +0000 Subject: [Histonet] Melanin Bleach In-Reply-To: References: Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C90A16313@PAEXCH1.PathologyAssociates.local> Hi Karen- I have also had problems getting tissue being stained for IHC markers to adhere to the slide. The Potassium permanganate is really harse on the tissue (if you can get the sections to stay on). Here is a trick I picked up at an NSH lecture that I have used successfully several times. Run your IHC stains as usual. Rinse well in water. Do not counterstain with hematoxylin. Stain with DiffQuik II for 30 seconds. Rinse in water for a short period of time. Differentiate with 10 dips each in 2 changes of 95% ETOH and 2 changes of 100% ETOH and then clear in xylene and coverslip. Results: melanin pigment will turn green. Brown DAB stain will be unaffected. Other tissue elements will be stained a medium blue color. This method will also work for other Special Stains but I have not attempted to modify this method for use on "Red" stains. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 10, 2016 8:45 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Melanin Bleach Good Morning, I need some help. Yesterday I bleached some heavily pigmented tissue. I have to run some IHC's on them. I bought the bleaching kit from American Master Tech. I had to put the Permanganate for about 6 hours to get the melanin and then a couple of minutes in Oxalic Acid. I had to let them set overnight because I could not get another IHC run in that day. It looks like the tissue fell off during decloaking. I used Apex slides. I rarely ever have to bleach anything here. So I am not sure if I did this correctly. I am thinking I need to bleach and do the run in the same day and not let them set over night in DiH20. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From rjbuesa at yahoo.com Tue May 10 11:36:54 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 10 May 2016 16:36:54 +0000 (UTC) Subject: [Histonet] Melanin Bleach In-Reply-To: References: Message-ID: <930716214.1612034.1462898214077.JavaMail.yahoo@mail.yahoo.com> You are right. Bleaching is a "rough" procedure for the "survival" of sections and if on top of that you left the section overnight in DiH2O that is a recipe for disaster, as the one you experienced. Try to do the whole procedure during the same day.Additionally it seems to me that 6h in potassium permanganate is too much, you should check the condition of the sections every hour trying to minimize KMnO4?action?the least time possible.Ren? On Tuesday, May 10, 2016 12:10 PM, "Heckford, Karen - SMMC-SF via Histonet" wrote: Good Morning, I need some help.? Yesterday I bleached some heavily pigmented tissue.? I have to run some IHC's on them.? I bought the bleaching kit from American Master Tech.? I had to put the Permanganate for about 6 hours to get the melanin and then a couple of minutes in Oxalic Acid.? I had to let them set overnight because I could not get another IHC run in that day.? It looks like the tissue fell off during decloaking.? ? I used Apex slides.? I rarely ever have to bleach anything here.? So I am not sure if I did this correctly.? I am thinking I need to bleach and do the run in the same day and not let them set over night in DiH20. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you." _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen at parrishmed.com Tue May 10 12:41:43 2016 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Tue, 10 May 2016 13:41:43 -0400 Subject: [Histonet] Cytology/Histology Staining Question In-Reply-To: <374DC72E6B29D44086F8FF3289351B2508823897@MSXMBXNSPRD39.acct.upmchs.net> References: <374DC72E6B29D44086F8FF3289351B2508823897@MSXMBXNSPRD39.acct.upmchs.net> Message-ID: <450B7A81EDA0C54E97C53D60F00776C323D9D2BCEA@isexstore03> Mary, We run both protocols on the same stainer, however, each protocol has it own set of reagents except for the common water wells and the two xylenes at the end before the final xylene. We have the Leica ST 5020, which has 36 wells not including the 2 oven wells. Hope this helps. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Mullen, Mary via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 10, 2016 10:55 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cytology/Histology Staining Question Hello all, I work in a small, low volume community hospital and was recently asked by a coworker why we do not just run both our cytology and histology slides on the same automated stainer (with their respective protocols). What I am wanting to know is if there is anyone currently running both staining protocols on a single automated stainer using common alcohols/xylenes/water? What are the pros/cons? Has there been any cross-contamination issues? We only run non-gyn cytology, all gyn cytology is sent out. Thanks, Mary K. Mullen, HTL(ASCP)CM Histotechnologist UPMC Northwest Seneca, PA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From thigginsht at msn.com Tue May 10 12:46:25 2016 From: thigginsht at msn.com (T H) Date: Tue, 10 May 2016 17:46:25 +0000 Subject: [Histonet] Cytology/Histology Staining Question (Mullen, Mary) In-Reply-To: References: Message-ID: Hey Mary, The problem is not the machine, it is the reagents sharing that is the issue. You can use different reagents and protocols for the Histo and Cyto slides and on the same instrument. You might even get away with changing your Alcohols and filtering everything else and see how that works. Try it and run some blank slides through the stainer and see if anything is there from the cytology specimens. I would personally have two separate sets of staining reagents to be on the safe side. Good luck!! Tim Message: 7 Date: Tue, 10 May 2016 14:54:55 +0000 From: "Mullen, Mary" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Cytology/Histology Staining Question Message-ID: <374DC72E6B29D44086F8FF3289351B2508823897 at MSXMBXNSPRD39.acct.upmchs.net> Content-Type: text/plain; charset="iso-8859-1" Hello all, I work in a small, low volume community hospital and was recently asked by a coworker why we do not just run both our cytology and histology slides on the same automated stainer (with their respective protocols). What I am wanting to know is if there is anyone currently running both staining protocols on a single automated stainer using common alcohols/xylenes/water? What are the pros/cons? Has there been any cross-contamination issues? We only run non-gyn cytology, all gyn cytology is sent out. Thanks, Mary K. Mullen, HTL(ASCP)CM Histotechnologist UPMC Northwest Seneca, PA From Lynn.O'Donnell at wchn.org Tue May 10 13:02:56 2016 From: Lynn.O'Donnell at wchn.org (O'Donnell, Lynn M.) Date: Tue, 10 May 2016 18:02:56 +0000 Subject: [Histonet] Cytology/Histology Staining Question (Mullen, Mary) In-Reply-To: References: Message-ID: This is the CAP regulation that talks about cross contamination. CYP.04150 Cross-Contamination Phase I There is a written procedure to prevent cross-contamination of specimens during processing and staining. NOTE: Procedures must prevent cross-contamination between gynecologic and non-gynecologic specimens. Also, procedures must prevent contamination among non-gynecologic cases when highly cellular specimens are processed. Methods to minimize this potential problem may include cytocentrifuge, filter, and monolayer preparations. Direct smears made from the sediment of highly cellular cases should be stained after the other cases, and the staining fluids must be changed or filtered between each of the highly cellular cases. One procedure to detect highly cellular specimens is to use a toluidine blue, or other rapid stain, on a wet preparation. One procedure to detect possible contamination is to insert a clean blank slide in each staining run and examine it for contamination. REFERENCES 1) Department of Health and Human Services, Centers for Medicare and Medicaid Services. Clinical laboratory improvement amendments of 1988; final rule. Fed Register. 2003(Jan 24):7169 [42CFR493.1274(b)(2-3)] Lynn M. O'Donnell, CT (ASCP), MHA l Technical Specialist, Cytology Danbury Hospital l lynn.o'donnell at wchn.org tel: 203-739-6704? Fax: 203-739-6034 -----Original Message----- From: T H via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 10, 2016 13:46 To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Cytology/Histology Staining Question (Mullen, Mary) Hey Mary, The problem is not the machine, it is the reagents sharing that is the issue. You can use different reagents and protocols for the Histo and Cyto slides and on the same instrument. You might even get away with changing your Alcohols and filtering everything else and see how that works. Try it and run some blank slides through the stainer and see if anything is there from the cytology specimens. I would personally have two separate sets of staining reagents to be on the safe side. Good luck!! Tim Message: 7 Date: Tue, 10 May 2016 14:54:55 +0000 From: "Mullen, Mary" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Cytology/Histology Staining Question Message-ID: <374DC72E6B29D44086F8FF3289351B2508823897 at MSXMBXNSPRD39.acct.upmchs.net> Content-Type: text/plain; charset="iso-8859-1" Hello all, I work in a small, low volume community hospital and was recently asked by a coworker why we do not just run both our cytology and histology slides on the same automated stainer (with their respective protocols). What I am wanting to know is if there is anyone currently running both staining protocols on a single automated stainer using common alcohols/xylenes/water? What are the pros/cons? Has there been any cross-contamination issues? We only run non-gyn cytology, all gyn cytology is sent out. Thanks, Mary K. Mullen, HTL(ASCP)CM Histotechnologist UPMC Northwest Seneca, PA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dawn.Gullifer at osumc.edu Tue May 10 13:11:23 2016 From: Dawn.Gullifer at osumc.edu (Gullifer, Dawn) Date: Tue, 10 May 2016 14:11:23 -0400 Subject: [Histonet] Open Position Message-ID: <9D6A52275061274B8ED422D3D84745B70F190B7B3A@EXMBOX-VP02.OSUMC.EDU> Hi- Job Opening: OSU Histology Lab, LLC Columbus, Ohio Assistant Laboratory Manager for a small reference based laboratory. Simple grossing and histology are performed. Musts: Graduate of a school of histology with a HT/HTL certification or equivalent. A minimum of an associate's degree in a science related field with a minimum of 24 semester hours (36 quarter hours) of biology, chemistry, physics and math. 3 years of full time histology technician experience in a clinical setting. 3 years of lead, supervisor or manager experience in an anatomic pathology/histology lab strongly desired. Contact: dawn.gullifer at osumc.edu Dawn Gullifer, BS, HT (ASCP) Clinical Laboratory and Outreach Manager Department of Pathology The Ohio State University Wexner Medical Center OSU Physicians Inc OSU Histology Lab, LLC 1507 Chambers Rd.Columbus.Ohio.43212 614.293.0358 Office 614.293.0345 Lab dawn.gullifer at osumc.edu CONFIDENTIALITY NOTICE: This e-mail, including attachments, may contain information that is physician-patient privileged, proprietary or otherwise confidential. If you are not the intended recipient or his or her authorized agent, use and disclosure of this message are prohibited. Any dissemination, distribution or copying of this e-mail is prohibited. If you received this e-mail in error, please notify the sender by replying to this e-mail and immediately delete the message and any attachments. From eridana at cox.net Tue May 10 14:04:33 2016 From: eridana at cox.net (Eridana) Date: Tue, 10 May 2016 12:04:33 -0700 Subject: [Histonet] p16 Nordi QC Message-ID: <20160510150433.3M0D6.17440.imail@fed1rmwml208> There is a fantastic resource for antibody assessment and optimization and they have done p16 as well as many many other antibodies. Their results of testing is free on line. p16 is http://www.nordiqc.org/Epitopes/p16/p16.htm from 2009 with many different companies antibodies. Here is the list of their epitopes http://www.nordiqc.org/epitopes.htm Donna Harclerode, HT, ASCP, HTL, QIHC Lead Histotechnologist VA San Diego, CA From Maxim_71 at mail.ru Tue May 10 14:14:51 2016 From: Maxim_71 at mail.ru (Maxim Peshkov) Date: Tue, 10 May 2016 22:14:51 +0300 Subject: [Histonet] Melanin Bleach Message-ID: <1604838626.20160510221451@mail.ru> Karen, The bleaching reagents will not compatible before IHC anyway. Some tips: 1. Do IHC-test as usual and counterstain nuclei with methylene blue Loeffler at 3-5 sec, only for profile of chromatine. The melanin will stain at green color. 2. Use any red color chromogen for IHC. It will be some contrast with brown melanine 3. Cut sections at 3 microns. Do all steps of IHC as usual, include DAB. All next steps do very gently: Before nuclear counterstain do bleaching procedure with 0.01% KMnO4 + 0.5% H2SO4 at 10 mins; gently rinse in DW 1 min; 0.5% oxalic acid 5-10 sec or up to bleaching; gently rinse in DW 1 min; counterstain nuclei and all other steps with regular manner of your lab. This solutions will not bleach DAB. Sections will not detach from slides. Hope this help. -- Maxim Peshkov Russia Taganrog. mailto:Maxim_71 at mail.ru From gachstetter at yahoo.com Tue May 10 15:01:21 2016 From: gachstetter at yahoo.com (Ginny Achstetter) Date: Tue, 10 May 2016 16:01:21 -0400 Subject: [Histonet] P24 Message-ID: Does anyone know of a supplier for P24 control slides? Sent from my iPhone From jaylundgren at gmail.com Tue May 10 16:44:35 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Tue, 10 May 2016 14:44:35 -0700 Subject: [Histonet] Melanin Bleach In-Reply-To: <1604838626.20160510221451@mail.ru> References: <1604838626.20160510221451@mail.ru> Message-ID: Cool trick Jeffrey! On Tue, May 10, 2016 at 12:14 PM, Maxim Peshkov via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Karen, > The bleaching reagents will not compatible before IHC anyway. > Some tips: > 1. Do IHC-test as usual and counterstain nuclei with methylene blue > Loeffler at 3-5 sec, only for > profile of chromatine. The melanin will stain at green color. > 2. Use any red color chromogen for IHC. It will be some contrast with > brown melanine > 3. Cut sections at 3 microns. Do all steps of IHC as usual, include DAB. > All next steps do very gently: > Before nuclear counterstain do bleaching procedure with 0.01% KMnO4 + 0.5% > H2SO4 at 10 mins; > gently rinse in DW 1 min; > 0.5% oxalic acid 5-10 sec or up to bleaching; > gently rinse in DW 1 min; > counterstain nuclei and all other steps with regular manner of your lab. > This solutions will not bleach DAB. > Sections will not detach from slides. > > Hope this help. > -- > Maxim Peshkov > Russia > Taganrog. mailto:Maxim_71 at mail.ru > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mcginnessjamie at yahoo.com Tue May 10 17:10:38 2016 From: mcginnessjamie at yahoo.com (Jamie McGinness) Date: Tue, 10 May 2016 17:10:38 -0500 Subject: [Histonet] Cryojane Oct artifact Message-ID: <0DC94EA3-2472-495D-BFDF-5B0BA8C1BF24@yahoo.com> I am getting a bubbly artifact on my tissue due to the OCT not being washed off the slide after putting it on a cryojane slide and flashing it and staining with H&E. Is there a trick to dissolve the OCT away Sent from my iPhone From tony.henwood at health.nsw.gov.au Tue May 10 19:32:52 2016 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 11 May 2016 00:32:52 +0000 Subject: [Histonet] Cytology/Histology Staining Question In-Reply-To: <374DC72E6B29D44086F8FF3289351B2508823897@MSXMBXNSPRD39.acct.upmchs.net> References: <374DC72E6B29D44086F8FF3289351B2508823897@MSXMBXNSPRD39.acct.upmchs.net> Message-ID: <0237449DE79DBC45B686AB82CDCD16FF01499DF7@SVDCMBX-MEX008.nswhealth.net> Yep, We do. We have a separate dehydration sequence for PAP and other special stains (separate from the eosin dehydration sequence). We have a Leica (used to be Vision Biosystems) Autostainer Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Mullen, Mary via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 11 May 2016 12:55 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cytology/Histology Staining Question Hello all, I work in a small, low volume community hospital and was recently asked by a coworker why we do not just run both our cytology and histology slides on the same automated stainer (with their respective protocols). What I am wanting to know is if there is anyone currently running both staining protocols on a single automated stainer using common alcohols/xylenes/water? What are the pros/cons? Has there been any cross-contamination issues? We only run non-gyn cytology, all gyn cytology is sent out. Thanks, Mary K. Mullen, HTL(ASCP)CM Histotechnologist UPMC Northwest Seneca, PA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From tony.henwood at health.nsw.gov.au Tue May 10 19:38:15 2016 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 11 May 2016 00:38:15 +0000 Subject: [Histonet] Melanin Bleach In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF01499E11@SVDCMBX-MEX008.nswhealth.net> The KMnO4/oxalic acid sequence will weaken the tissue adherence. If you do not have to use Peroxidase/DAB, then I would recommend alkaline phosphatase labelling (+red chromagen). Remember KMnO4/oxalic acid can be deleterious to some antigen-antibody reactions. We found that LCA, CAM5.2, L26, alpha-fetoprotein, and NSE were labile, whilst alpha-1-antitrypsin, HBsAg, CMV, CEA, Thyroglobin and chromogranin showed decreased staining. EMA, desmin, HSVII, S100, GFAP, PSA, PAcP, Calcitonin, ACTH and Prolactin were found to be resistant to permanganate treatment. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 11 May 2016 1:45 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Melanin Bleach Good Morning, I need some help. Yesterday I bleached some heavily pigmented tissue. I have to run some IHC's on them. I bought the bleaching kit from American Master Tech. I had to put the Permanganate for about 6 hours to get the melanin and then a couple of minutes in Oxalic Acid. I had to let them set overnight because I could not get another IHC run in that day. It looks like the tissue fell off during decloaking. I used Apex slides. I rarely ever have to bleach anything here. So I am not sure if I did this correctly. I am thinking I need to bleach and do the run in the same day and not let them set over night in DiH20. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From aeck at dh.org Wed May 11 05:38:26 2016 From: aeck at dh.org (Eck, Allison) Date: Wed, 11 May 2016 10:38:26 +0000 Subject: [Histonet] open position in education Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A9723BBF4@DH-MAIL01.dhorg.org> We have an opening for a clinical coordinator for the histotechnician program at Harcum College in Bryn Mawr, PA Please direct all inquires to Donna Broderick at dbroderick at harcum.edu http://www.harcum.edu/s/1044/images/editor_documents/new_site/about_us/human_resources/jobs/histotechnician_-_2016.pdf Harcum College in Bryn Mawr, PA, a leader among the nation?s independent, two year colleges, has an immediate opening for a Clinical Coordinator for the Histotechnician Program. This is a part-time (24 hours/week) non-benefits eligible position. Responsibilities: ? Monitor student progress in completing competences for each of the four practicum courses: ? Grade all practicum checklists and enter checklist grades in the Learning Management System: Webstudy ? Grade all practicum stain reports and slide cutting reports. Enter grades into Webstudy ? Print and review practicum journals and save for review at department and advisory board meetings ? Collect and grade all practicum evaluations, enter grade in Webstudy ? Schedule and attend student case study presentations at clinical sites and enter case study grade in Webstudy ? Document all communication with each clinical affiliate ? Acquire additional clinical affiliates as needed for the student practicum experience ? Schedule and attend annual clinical site meetings, submit meeting minutes to HT Program Director ? Order stain kits for all clinical sites for student use as needed each semester ? Coordinate student uniform ordering ? Track completion of Student Medical/Background Clearances ? Evaluate effectiveness of the practicum educational experience in meeting criteria/competencies for an entry level Histotechnician per the National Accrediting Agency for Clinical Laboratory Science (NAACLS) ? Participate in and document annual continuing education activities ? Work with HT Program Director, Education Coordinator, and Practicum Coordinator to evaluate program outcomes and recommend improvements as needed ? Review annual program goals and student learning outcomes with HT Program Director ? Participate as a NAACLS volunteer to review self-study reports and attend site visits ? Provide input in annual budget reports Qualifications/Skills: ? ASCP certified as an HTL with a minimum of 5-years experience in a Histology Laboratory ? Current membership with American Society of Clinical Pathologists (ASCP) and/or National Society for Histotechnology ? Teaching experience preferred ? Experience with online education preferred ABOUT HARCUM COLLEGE Harcum College is an independent, coeducational, fully accredited, residential college. The College offers associate degrees in allied health, business and social science, as well as certificates and a variety of continuing studies programs. Founded in 1915 by Edith Hatcher Harcum and Octavius Harcum, it was the first college in Pennsylvania chartered by the state to offer the associate degree. Today Harcum has degree programs on its Bryn Mawr Campus, located about 20 minutes west of Philadelphia, programs online and at Partnership Sites throughout the region, serving approximately 1,500 students. Harcum provides quality instruction from faculty, close student-faculty collaboration, small class sizes, and individualized support services. COMPENSATION Harcum College offers a comprehensive benefits package and the salary will be commensurate with experience. Harcum College is an Equal Employment Opportunity employer. Resumes MUST include salary requirements for consideration of employment. Please send cover letter and resume including work history to: Claudine Vita Executive Director, Human Resources & Compliance Officer Harcum College 750 Montgomery Avenue Bryn Mawr, PA 19010 Fax: 610-526-6011 Preferred response for this position is via email: cvita at harcum.edu Allison Eck, HTL(ASCP)cm, AHI(AMT) Lead Tech Histology Doylestown Hospital 595 W State St Doylestown, PA 18901 215-345-2264 aeck at dh.org From Karen.Heckford at DignityHealth.org Wed May 11 07:05:13 2016 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Wed, 11 May 2016 05:05:13 -0700 Subject: [Histonet] PathNet HELP Message-ID: Good Morning, I need to contact some of you. If you have Histology in-house and use the PathNet program. I have some questions on how you handle Inventory Management and a couple of other things. If it is okay to give you a ring please let me know. Thank you so much, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From Karen.Heckford at DignityHealth.org Wed May 11 07:12:04 2016 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Wed, 11 May 2016 05:12:04 -0700 Subject: [Histonet] PathNet HELP UPDATED Message-ID: Good Morning, I need to contact some of you. If you have Histology in-house, use the PathNet program and do CAP. I have some questions on how you handle Inventory Management and a couple of other things. If it is okay to give you a ring please let me know. Thank you so much, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From kenneth.metzger at aruplab.com Wed May 11 09:57:30 2016 From: kenneth.metzger at aruplab.com (Metzger, Kenneth) Date: Wed, 11 May 2016 14:57:30 +0000 Subject: [Histonet] Plastic to Paraffin Message-ID: <3855827CD3E36249A30D57F6F896F8F1023837C88D@EXMBX2.aruplab.net> Hello All, Our EM lab melted a block meant for IHC staining and put it into plastic. Is there any way to get the tissue back to paraffin? I know it's a long shot but just wondering.... Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metzger at aruplab.com ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From Timothy.Morken at ucsf.edu Wed May 11 10:07:42 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 11 May 2016 15:07:42 +0000 Subject: [Histonet] Plastic to Paraffin In-Reply-To: <3855827CD3E36249A30D57F6F896F8F1023837C88D@EXMBX2.aruplab.net> References: <3855827CD3E36249A30D57F6F896F8F1023837C88D@EXMBX2.aruplab.net> Message-ID: <761E2B5697F795489C8710BCC72141FF6FD55E77@ex07.net.ucsf.edu> Kenneth, Sorry but no, it does not work to go the other way. We do this occasionally but normally only a take a small portion of the tissue in the block. Did they embed all of it in plastic? Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Metzger, Kenneth via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, May 11, 2016 7:58 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Plastic to Paraffin Hello All, Our EM lab melted a block meant for IHC staining and put it into plastic. Is there any way to get the tissue back to paraffin? I know it's a long shot but just wondering.... Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metzger at aruplab.com ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mprice63 at live.com Wed May 11 10:59:17 2016 From: mprice63 at live.com (Marsha Price) Date: Wed, 11 May 2016 15:59:17 +0000 Subject: [Histonet] Florida State Licensure In-Reply-To: References: Message-ID: Can someone provide the link for the application to obtain HT Florida State Licensure Sent from my iPhone > On May 10, 2016, at 12:00 PM, wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. IHC Wet Workshop announcement (Ihc Workshop) > 2. Re: PAS Stain (Seeley, Heather) > 3. Re: Tracking surgical specimens (Jamieson Anderson) > 4. Tips on staining PLA2R assay on Leica Bond (Eddie Martin) > 5. Re: PAS Stain (victor_tobias at comcast.net) > 6. P16 (Charles Riley) > 7. Cytology/Histology Staining Question (Mullen, Mary) > 8. Re: Cytology/Histology Staining Question (Rene J Buesa) > 9. ASCP Certified Pathologist Assistant Job Opening- (Melissa Owens) > 10. Melanin Bleach (Heckford, Karen - SMMC-SF) > 11. Re: Melanin Bleach (Jeffrey Robinson) > 12. Re: Melanin Bleach (Rene J Buesa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 9 May 2016 10:16:39 -0700 > From: Ihc Workshop > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] IHC Wet Workshop announcement > Message-ID: <3FAC1E78-7C1B-4CBC-9A99-FDA3F2BAF430 at gmail.com> > Content-Type: text/plain; charset=us-ascii > > June 23 & 24, 2016. San Francisco Bay Area > This IHC lab course is aimed at hands-on training of attendees in all aspects of IHC staining of human, mouse and animal tissues with varied antibodies. This is a small group workshop and is taught in a lab.....Lots of troubleshooting, only few spots left. > To get more detail contact. Maria at: ihcworkshop at gmail.com > > > > > ------------------------------ > > Message: 2 > Date: Mon, 9 May 2016 17:35:02 +0000 > From: "Seeley, Heather" > To: Bob Richmond , > "Histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] PAS Stain > Message-ID: > <9DFE334E776E734D9D231EF60CCE93C57E26DB05 at TENHDCTHMB10-31.tenethealth.net> > > Content-Type: text/plain; charset="us-ascii" > > We have one girl that always spits on our slide! :) works great! > > HEATHER SEELEY, HT(ASCP) > Histotech > 803-985-4676 OFFICE > 803-327-7598 FAX > > > ________________________________________ > From: Bob Richmond [rsrichmond at gmail.com] > Sent: Thursday, May 05, 2016 2:10 PM > To: Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] PAS Stain > > Amylase (diastase) for the PAS stain queries: > > Whatever happened to spitting on the slide (30 min at room temperature)? > John Kiernan advises "thinking of lemons and drooling into a small beaker" > though I'd advise chewing on a rubber band for a few seconds. > > He notes that alpha amylase is preferred. I'd go with the cheapest one in > the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma > offers a heat-stable alpha amylase. > > Bob Richmond > Samurai Pathologist > Maryville TN > > > ------------------------------ > > Message: 3 > Date: Mon, 9 May 2016 17:41:09 +0000 (UTC) > From: Jamieson Anderson > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Tracking surgical specimens > Message-ID: > <537327FDD6BA9F6C.0B786CD3-7FD7-4574-B1DC-3D754EACB582 at mail.outlook.com> > > Content-Type: text/plain; charset=us-ascii > > Hi Lynne, > In our lab we have a log book at Accessioning that each physician/nurse/porter/MOA signs when dropping off specimens. We also encourage them to drop off a QC sheet with a patient label for each specimen that is dropped off. We check these to ensure we have received each specimen, and we date/time stamp them and keep them on record in case we need to refer back to them in the future (ie. In case a clinician claims they dropped off a specimen we can prove they did not). > Jamieson AndersonTechnical Coordinator - Anatomic PathologySt. Paul's HospitalLower Mainland Pathology & Laboratory Medicine > > _____________________________ > > Date: Mon, 9 May 2016 14:49:56 +0000 > From: "Bell, Lynne" > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > Subject: [Histonet] Tracking surgical specimens brought to the lab > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > For those of you who have specimens brought to your lab: do you have the person dropping off the specimen(s) initial a manifest to keep track of specimens actually dropped off. Case in point - a physician's office says they dropped off a specimen and we have no record of it being accessioned. I would like to track specimens dropped off and I am not sure how to accomplish this. > > I appreciate any feedback! > > Thanks, > > > Lynne Bell, HT (ASCP) > Histology Team Leader > Central Vermont Medical Center > 130 Fisher Road > Berlin, VT 05641 > (802)371-4923 > > > > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 150, Issue 12 > ***************************************** > > > > > > ------------------------------ > > Message: 4 > Date: Mon, 9 May 2016 12:51:46 -0500 > From: Eddie Martin > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Tips on staining PLA2R assay on Leica Bond > Message-ID: <16EDC8EC-EDAB-4F63-A3BD-BEC24EAB0BE3 at gmail.com> > Content-Type: text/plain; charset=utf-8 > > I don?t do staining for this antibody, but have experience with IMF on frozen tissue and FFPE and have worked with the Leica BOND max and BOND-3. If you would prefer IHC over IMF, you can search for a human anti-mouse, or human anti-rabbit antibody. Abcam provides a polyclonal whole serum antibody that works on FFPE. I grabbed this from their website: > Anti-PLA2R antibody (ab80054) > > Since Abcam?s PLA2R antibody is a rabbit anti-human, you would need to create a modified DAB or modified RED protocol that doesn?t include the secondary antibody / linker step in your protocol prior to dispensing polymer. The rest of your protocol can remain as it is. > > As you are using a Leica BOND, both ways are actually pretty easy to set up, either indirect IMF or using Leica?s DAB kit for IHC. You would just need to play around with retrieval times with both Citrate and EDTA to get the optimum staining pattern. Additional ancillaries may be added when necessary should you have any background stain that is hard to get out. > > Best Regards, > > Eddie Martin, HT(ASCP), QIHC > 954-826-9403 > Edmartin26 at gmail.com > > ------------------------------ > > Message: 5 > Date: Mon, 9 May 2016 22:39:38 +0000 (UTC) > From: victor_tobias at comcast.net > To: Heather Seeley , > "Histonet at lists.utsouthwestern.edu" > , Bob Richmond > > Subject: Re: [Histonet] PAS Stain > Message-ID: > <1237907839.22424188.1462833578398.JavaMail.zimbra at comcast.net> > Content-Type: text/plain; charset="utf-8" > > > > ------------------------------ > > Message: 6 > Date: Tue, 10 May 2016 05:47:18 -0400 > From: Charles Riley > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] P16 > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Has anyone found a way to do p16 staining without purchasing anything from > Ventana? > > My company wants to do P16 but refuses to by any ventana products and I > have explained it is a waste of money to keep testing the antibodies from > all the other companies. > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > > > ------------------------------ > > Message: 7 > Date: Tue, 10 May 2016 14:54:55 +0000 > From: "Mullen, Mary" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Cytology/Histology Staining Question > Message-ID: > <374DC72E6B29D44086F8FF3289351B2508823897 at MSXMBXNSPRD39.acct.upmchs.net> > > Content-Type: text/plain; charset="iso-8859-1" > > Hello all, > > > > I work in a small, low volume community hospital and was recently asked by a coworker why we do not just run both our cytology and histology slides on the same automated stainer (with their respective protocols). > > > > What I am wanting to know is if there is anyone currently running both staining protocols on a single automated stainer using common alcohols/xylenes/water? What are the pros/cons? Has there been any cross-contamination issues? > > > > We only run non-gyn cytology, all gyn cytology is sent out. > > > > > > > > Thanks, > > > > Mary K. Mullen, HTL(ASCP)CM > Histotechnologist > UPMC Northwest > Seneca, PA > > > > ------------------------------ > > Message: 8 > Date: Tue, 10 May 2016 15:07:04 +0000 (UTC) > From: Rene J Buesa > To: "Mullen, Mary" , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Cytology/Histology Staining Question > Message-ID: > <1252522947.1519943.1462892824143.JavaMail.yahoo at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > I would be concerned with potential cross-contamination. In my lab we had 2 staining instruments, one for cytology and other for histology.Ren? > > On Tuesday, May 10, 2016 10:59 AM, "Mullen, Mary via Histonet" wrote: > > > Hello all, > > > > I work in a small, low volume community hospital and was recently asked by a coworker why we do not just run both our cytology and histology slides on the same automated stainer (with their respective protocols). > > > > What I am wanting to know is if there is anyone currently running both staining protocols on a single automated stainer using common alcohols/xylenes/water? What are the pros/cons? Has there been any cross-contamination issues? > > > > We only run non-gyn cytology, all gyn cytology is sent out. > > > > > > > > Thanks, > > > > Mary K. Mullen, HTL(ASCP)CM > Histotechnologist > UPMC Northwest > Seneca, PA > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 9 > Date: Tue, 10 May 2016 15:44:21 +0000 > From: Melissa Owens > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] ASCP Certified Pathologist Assistant Job Opening- > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Hello, > > I have a Full Time/Permanent job opening for an ASCP certified Pathologist Assistant in Ohio. Please contact me for details. Have a great day! > > Melissa Owens > President, Laboratory Staffing > Allied Search Partners > > > T: 888.388.7571 ext. 102 > > F: 888.388.7572 > > > > ------------------------------ > > Message: 10 > Date: Tue, 10 May 2016 08:44:33 -0700 > From: "Heckford, Karen - SMMC-SF" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Melanin Bleach > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > I need some help. Yesterday I bleached some heavily pigmented tissue. I have to run some IHC's on them. I bought the bleaching kit from American Master Tech. I had to put the Permanganate for about 6 hours to get the melanin and then a couple of minutes in Oxalic Acid. I had to let them set overnight because I could not get another IHC run in that day. It looks like the tissue fell off during decloaking. I used Apex slides. I rarely ever have to bleach anything here. So I am not sure if I did this correctly. I am thinking I need to bleach and do the run in the same day and not let them set over night in DiH20. > > Thanks, > > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford at dignityhealth.org > Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." > > > > > > ------------------------------ > > Message: 11 > Date: Tue, 10 May 2016 16:33:26 +0000 > From: Jeffrey Robinson > To: "Heckford, Karen - SMMC-SF" > Cc: "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Melanin Bleach > Message-ID: > <204A03EB5A7F0A4BB1EEDD52A963829C90A16313 at PAEXCH1.PathologyAssociates.local> > > Content-Type: text/plain; charset="us-ascii" > > Hi Karen- I have also had problems getting tissue being stained for IHC markers to adhere to the slide. The Potassium permanganate is really harse on the tissue (if you can get the sections to stay on). Here is a trick I picked up at an NSH lecture that I have used successfully several times. > > Run your IHC stains as usual. Rinse well in water. Do not counterstain with hematoxylin. Stain with DiffQuik II for 30 seconds. Rinse in water for a short period of time. Differentiate with 10 dips each in 2 changes of 95% ETOH and 2 changes of 100% ETOH and then clear in xylene and coverslip. > Results: melanin pigment will turn green. Brown DAB stain will be unaffected. Other tissue elements will be stained a medium blue color. > This method will also work for other Special Stains but I have not attempted to modify this method for use on "Red" stains. > > Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. > > -----Original Message----- > From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, May 10, 2016 8:45 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Melanin Bleach > > Good Morning, > I need some help. Yesterday I bleached some heavily pigmented tissue. I have to run some IHC's on them. I bought the bleaching kit from American Master Tech. I had to put the Permanganate for about 6 hours to get the melanin and then a couple of minutes in Oxalic Acid. I had to let them set overnight because I could not get another IHC run in that day. It looks like the tissue fell off during decloaking. I used Apex slides. I rarely ever have to bleach anything here. So I am not sure if I did this correctly. I am thinking I need to bleach and do the run in the same day and not let them set over night in DiH20. > > Thanks, > > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford at dignityhealth.org > Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. > > > > ------------------------------ > > Message: 12 > Date: Tue, 10 May 2016 16:36:54 +0000 (UTC) > From: Rene J Buesa > To: "Heckford, Karen - SMMC-SF" , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Melanin Bleach > Message-ID: > <930716214.1612034.1462898214077.JavaMail.yahoo at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > You are right. Bleaching is a "rough" procedure for the "survival" of sections and if on top of that you left the section overnight in DiH2O that is a recipe for disaster, as the one you experienced. Try to do the whole procedure during the same day.Additionally it seems to me that 6h in potassium permanganate is too much, you should check the condition of the sections every hour trying to minimize KMnO4?action?the least time possible.Ren? > > On Tuesday, May 10, 2016 12:10 PM, "Heckford, Karen - SMMC-SF via Histonet" wrote: > > > Good Morning, > I need some help.? Yesterday I bleached some heavily pigmented tissue.? I have to run some IHC's on them.? I bought the bleaching kit from American Master Tech.? I had to put the Permanganate for about 6 hours to get the melanin and then a couple of minutes in Oxalic Acid.? I had to let them set overnight because I could not get another IHC run in that day.? It looks like the tissue fell off during decloaking.? ? I used Apex slides.? I rarely ever have to bleach anything here.? So I am not sure if I did this correctly.? I am thinking I need to bleach and do the run in the same day and not let them set over night in DiH20. > > Thanks, > > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford at dignityhealth.org > ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you." > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 150, Issue 13 > ***************************************** From tbraud at holyredeemer.com Wed May 11 11:04:15 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 11 May 2016 16:04:15 +0000 Subject: [Histonet] Histo/Cyto staining Message-ID: <48E053DDF6CE074DB6A7414BA05403F8072972@HRHEX02-HOS.holyredeemer.local> We run both stains on our Sakura Prisma, but they share no common reagents except for the final Xylene unload station. We do use the combination CytoKwik stain, which combines the OG6 and EA50 steps, to reduce the number of steps in our Cytology stain procedure. We only run non-gyn, too. It is sooooo much easier! Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Message: 7 Date: Tue, 10 May 2016 14:54:55 +0000 From: "Mullen, Mary" Subject: [Histonet] Cytology/Histology Staining Question Hello all, I work in a small, low volume community hospital and was recently asked by a coworker why we do not just run both our cytology and histology slides on the same automated stainer (with their respective protocols). What I am wanting to know is if there is anyone currently running both staining protocols on a single automated stainer using common alcohols/xylenes/water? What are the pros/cons? Has there been any cross-contamination issues. We only run non-gyn cytology, all gyn cytology is sent out. Thanks, Mary K. Mullen, HTL(ASCP)CM Histotechnologist UPMC Northwest Seneca, PA From relia1 at earthlink.net Wed May 11 11:40:27 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 11 May 2016 12:40:27 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin 5-11-2016 I hope you had a nice Mother's Day! Message-ID: <006f01d1aba3$d30c9090$7925b1b0$@earthlink.net> Hi Histonetters! I hope you had a lovely Mother?s Day!!! Can you believe it? Summer is almost here!!! My phone has been ringing off the hook this past week with some exciting new opportunities. This is a quick update of the positions I am working on that I am most excited about. Some are brand new and some are additional positions where I have placed people who love their new jobs. As you know all of the positions I work with are full-time permanent positions with the best hospitals, labs and clinics. I only work with clients that offer excellent compensation including competitive salaries, great benefits and relocation assistance /sign on bonuses. Spotlight Opportunities: Virginia Beach, VA ? Histology Tech (15K sign on bonus) Chicagoland ? Histology Technician 3K sign on bonus! Amarillo, TX ? Histology Manager ? due to growth Austin, TX ? HistoTech One of Austin?s top employers! Here is a list of the rest of the positions I am excited to tell you about: Tyler, TX - Histology Technician- DAYS-state of the art lab! Kansas City, KS - Dermpath Histotech ? Learn MOHS! Fayetteville, AR -Lead Dermpath Histotech ? Run your own Lab! Dallas, TX ? Histology Supervisor ? Growing Private Lab! Roswell, NM ? Assistant Supervisor ? Exciting opportunity! Los Angeles, CA IHC Tech Support ? ?Get off the bench? If you are interested in any of these opportunities contact me ASAP!!!! My clients are eager to interview and hire NOW!!!! If you are interested in any of these positions please call me toll free at 866-607-3542, on my cell at 407-353-5070 or e-mail me at relia1 at earthlink.net with your resume and a number where I can reach you at a time that is convenient for you. If you are interested in looking into new job opportunities in other areas that are not mentioned above please contact me as well. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember It never hurts to keep an eye open even if you are happy in your present job. Also if you know anyone else that might be interested I would really appreciate it if you would pass my information along to them as well. If I place someone you refer you will earn a referral fee. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From vcaldwell4232 at gmail.com Wed May 11 13:11:02 2016 From: vcaldwell4232 at gmail.com (Van Caldwell) Date: Wed, 11 May 2016 11:11:02 -0700 Subject: [Histonet] Open Position in the Santa Barbara California area Message-ID: Diagnostic Services is looking for an experienced technology leader with a strong medical device hardware and software background to deliver technical support activities for our pathology/histology instrumentation solutions. We develop pathology solutions for tissue-based cancer diagnostics. Our portfolio of solutions include fully automated systems, all operating in a highly-regulated market environment. We are using lean lab approaches to increase our customer's productivity and efficiency while simultaneously eliminating the chances of operational errors in their workflows. This is an opportunity to join an enthusiastic and dynamic team and work closely with application experts, hardware experts, marketing, QA/RA and directly with customers. Responsibilities include: - Provides through telephone or other communication tools general information of a technical and occasionally non-technical nature, to Agilent existing and potential customers. - Monitor Service help desk and phone queue to respond to inquiries and requests for support. - Document all complaints or reportable events per QMS procedures. - Provides escalation support to internal resources including Technical Support, Field Service and Application Specialists. - Troubleshoot IT and instrument issues using various methods including remote access and reviewing the instrument's diagnostic files. - Serve as a primary conduit of information back to global IT team for troubleshooting, problem investigation and resolution of instrument hardware and software issues. - Evaluates assignments and determines actions required to provide technical support service. - Duties involve analysis and new/ customized approaches. - Solves complex problems requiring technical and business admin breadth/ depth of knowledge. Qualifications: - Bachelor?s degree, specialized training/certification, or equivalent combination of education and experience. - 5+ year?s relevant experience in similar position. - Extensive troubleshooting knowledge and skills to complete specialized tasks. - Experience with Laboratory Information Systems, Laboratory Equipment, Laboratory Workflow, Networks and Server Administration is a plus Here is the job link to upload your CV. https://www.agilent.apply2jobs.com/ProfExt/index.cfm?fuseaction=mExternal.showJob&RID=2066236&CurrentPage=1 Agilent Technologies Inc. is an equal opportunity employer. Qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, disability or any other protected categories under all applicable laws. ------------------------------------------------ *Jason R. Bennett* Manager US Technical Support Group Agilent Technologies, Inc. 1170 Mark Ave Carpinteria, CA 93103 Direct +1 805.318.6110 (Telnet 318.6110) Tech +1 800.235.5763 Option #2 Jason.Bennett at agilent.com www.agilent.com From c.tague at Pathologyarts.com Wed May 11 15:18:52 2016 From: c.tague at Pathologyarts.com (Curt) Date: Wed, 11 May 2016 20:18:52 +0000 Subject: [Histonet] embedding and microtomy "medical waste" Message-ID: <9C8F910F72893643B3C3793C3D67132B67CBAFD6@PATHOLOGYSERVER.pathologyarts.local> So here's a good one for you all... we had the county health department come through the lab and ding us on medical waste... specifically the plastic disposable lids at embedding, the lens paper used for wrapping specimens such as ECC and EMB. Then they got us on the Kim Wipes used to clean the water bath at microtomy... those papers have human tissue on them so they need to be treated as medical waste... NOW we have to have red cans next to all microtomes and embedding stations. The obvious issue outside of cost and logic is that these medical waste cans all seem to have self closing lids which really interferes with the rhythm and pace of work when one needs to reach over with a food to open the lid after every block is embedded and when they water bath is cleaned after every block... Simple question(s): 1)does anyone else have to do such things to contain the waste, 2) does anyone know of a source for medical waste cans that do not have these frustrating self closing lids... if we could simple remove the lid and replace it when done then we could deal with it, the cost is one thing but slowing down work flow is a problem. And just for a little more humor, they actually wanted me to contain and dispose of the water runoff from our two automated slide stainers, we run about 2200 slides a night... that would be many gallons of waste water every night and would not be within the budget.... We in turn ran a fish kill test which demonstrated that the water runoff which contain little Hematoxylin, bluing and clarifier do not pose any significant threat to the environment, not even in California.... Bottom line to all this, I need some red trash cans with removable lids, if they're still out there somewhere.... Anywhere..... Thanks for your input, Curt Ps, I didn't proff read thie smail... if something is not spelt correctly, don't hold it against me.... From thoward at unm.edu Wed May 11 15:34:27 2016 From: thoward at unm.edu (Tamara Howard) Date: Wed, 11 May 2016 20:34:27 +0000 Subject: [Histonet] plastic to paraffin Message-ID: Kenneth - You can do this, but it won't be pretty - if you trim as much of the plastic away as you can, you can etch the resin away with sodium methoxide (or ethoxide): http://www.ncbi.nlm.nih.gov/pubmed/357729 [http://www.ncbi.nlm.nih.gov/coreutils/img/pubmed256blue.png] A method for the removal of epoxy resins from tissue in preparation for scanning electron microscopy. - PubMed - NCBI www.ncbi.nlm.nih.gov J Microsc. 1978 May;113(1):95-9. Good luck! Tamara ................................................... Tamara Howard Dept. of Cell Biology & Physiology University of New Mexico Albuquerque, NM From Timothy.Morken at ucsf.edu Wed May 11 16:36:52 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 11 May 2016 21:36:52 +0000 Subject: [Histonet] plastic to paraffin In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF6FD56142@ex07.net.ucsf.edu> Tamara, Yes, it is possible, and some do immuno on plastic sections with this method, but it's a lot of work and the issue arises of whether you have validated your stains to work when this method is used. And do you have controls treated the same way? In the clinical diagnostic environment, in purely practical terms, it is not worthwhile. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Tamara Howard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, May 11, 2016 1:34 PM To: kenneth.metzger at aruplab.com Cc: histonet at lists.utsouthwestern.edu Subject: [Histonet] plastic to paraffin Kenneth - You can do this, but it won't be pretty - if you trim as much of the plastic away as you can, you can etch the resin away with sodium methoxide (or ethoxide): http://www.ncbi.nlm.nih.gov/pubmed/357729 [http://www.ncbi.nlm.nih.gov/coreutils/img/pubmed256blue.png] A method for the removal of epoxy resins from tissue in preparation for scanning electron microscopy. - PubMed - NCBI www.ncbi.nlm.nih.gov J Microsc. 1978 May;113(1):95-9. Good luck! Tamara ................................................... Tamara Howard Dept. of Cell Biology & Physiology University of New Mexico Albuquerque, NM _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Wed May 11 17:19:25 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 11 May 2016 22:19:25 +0000 Subject: [Histonet] embedding and microtomy "medical waste" In-Reply-To: <9C8F910F72893643B3C3793C3D67132B67CBAFD6@PATHOLOGYSERVER.pathologyarts.local> References: <9C8F910F72893643B3C3793C3D67132B67CBAFD6@PATHOLOGYSERVER.pathologyarts.local> Message-ID: <761E2B5697F795489C8710BCC72141FF6FD56199@ex07.net.ucsf.edu> Curt, we went through the same thing, but for the embedding we keep a plastic bucket on the bench and lined with a biohazard bag, and put all the lids and papers in that. We keep those for a week in case some tissue ends up missing. Then it goes into the red cans. At the microtome we do use the smallish plastic biohazard waste cans with self-closing lid. (https://www.fishersci.com/shop/products/eagle-step-on-biohazard-waste-containers-3/p-36261) They are now used to this and it is not really a problem. They are not allowed to prop them open. We tried red 5-gallon "paint can" type containers with fully removable lids, but the techs were required to put the lids on every time they moved away from the microtome. Of course that did not happen. Our safety people finally said we must have self-closing lids. We tried several kinds, even one that was auto-open with a sensor, but that did not open fast enough. The foot pedal works ok. We went through the stainer water issue as well when we were considering the Ventana stainer that disposes directly to the drain. The city water department came in and checked out everything and passed it all off. He also gave us a lot of good info about what we can and cannot put down the drain here. Best to contact local authorities on that since it may be different than ours. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Curt via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, May 11, 2016 1:19 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] embedding and microtomy "medical waste" So here's a good one for you all... we had the county health department come through the lab and ding us on medical waste... specifically the plastic disposable lids at embedding, the lens paper used for wrapping specimens such as ECC and EMB. Then they got us on the Kim Wipes used to clean the water bath at microtomy... those papers have human tissue on them so they need to be treated as medical waste... NOW we have to have red cans next to all microtomes and embedding stations. The obvious issue outside of cost and logic is that these medical waste cans all seem to have self closing lids which really interferes with the rhythm and pace of work when one needs to reach over with a food to open the lid after every block is embedded and when they water bath is cleaned after every block... Simple question(s): 1)does anyone else have to do such things to contain the waste, 2) does anyone know of a source for medical waste cans that do not have these frustrating self closing lids... if we could simple remove the lid and replace it when done then we could deal with it, the cost is one thing but slowing down work flow is a problem. And just for a little more humor, they actually wanted me to contain and dispose of the water runoff from our two automated slide stainers, we run about 2200 slides a night... that would be many gallons of waste water every night and would not be within the budget.... We in turn ran a fish kill test which demonstrated that the water runoff which contain little Hematoxylin, bluing and clarifier do not pose any significant threat to the environment, not even in California.... Bottom line to all this, I need some red trash cans with removable lids, if they're still out there somewhere.... Anywhere..... Thanks for your input, Curt Ps, I didn't proff read thie smail... if something is not spelt correctly, don't hold it against me.... _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garethdavisyuma at gmail.com Wed May 11 17:53:51 2016 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Wed, 11 May 2016 15:53:51 -0700 Subject: [Histonet] Medical waste in Arizona Message-ID: Does anyone know if Arizona considers the cassette lids, bio bags and kim wipes, used on water baths, as medical waste. The recent post about being dinged for not putting those in medical waste, was the first I heard of anything like that. But was hoping that was just California. -- Gareth B. Davis, HT From boneslides at aol.com Thu May 12 10:18:36 2016 From: boneslides at aol.com (boneslides at aol.com) Date: Thu, 12 May 2016 11:18:36 -0400 Subject: [Histonet] boneslides Message-ID: <154a58c4bcd-1f3-142fa@webprd-m64.mail.aol.com> http://whattheartsmean.org/dpxbdj-723.php From: boneslides Code: gh17a8af88rwy95clglr Time: 5/12/2016 4:18:35 PM From vcaldwell4232 at gmail.com Thu May 12 11:41:16 2016 From: vcaldwell4232 at gmail.com (Van Caldwell) Date: Thu, 12 May 2016 09:41:16 -0700 Subject: [Histonet] Open Position in Santa Clara California For IHC Application Specialist Message-ID: Date Posted: 11/May/16 Requisition: 2066078 Job Title: Immuno-Histo Chemistry Applications Specialist Description: Are you looking to take your lab experience in Immuno-Histo Chemistry to the next level? To utilize your expertise to support the needs of our diverse internal and external customers in an exciting, dynamic role. Whether you are still working the bench or have since moved to other responsibilities, this exciting opportunity with Agilent will afford you the opportunity to capitalize on your experience and share your knowledge! Be a part of our growing internal education team where your technical and scientific expertise can be leveraged. Agilent is a leader in life sciences, diagnostics and applied chemical markets. We provide laboratories worldwide with instruments, services, consumables, applications and expertise, enabling customers to gain the insights they seek. Agilent gives doctors a head start in the fight against cancer and other diseases. Our solutions help pathology laboratories deliver fast, accurate information and help medical professionals make more precise diagnoses so patients can receive the most effective therapies. As an Immuno-Histo Chemistry Applications Specialist, your responsibilities will include acting as a subject matter expert for our internal staff and external customers. Responsibilities would include the delivery of instruction, mentoring and support from both an educational and technical perspective; assisting in translating customer's educational requirements into single seminars, customized solutions or projects, which improve the effectiveness and intellectual capital for our customers. Will also work with customers and internal organizations to develop specifications, and design and conduct technical training courses for customers and/or employees. Other tasks/ responsibilities may include: ? Leading participants in classroom lectures and/or laboratory training sessions. ? Providing support through technical communication with internal staff, field based representatives, customers and end users. ? Assist in developing new and novel methods of teaching relevant industry topics. ? Utilizing internal systems to place orders for regents, tissue and other supplies necessary to perform tasks. Qualifications: ? 5+ years relevant experience including hands on laboratory experience and strong pathology, technical, and instrument background. ? Post Graduate, Masters Degree, Bachelors Degree and/or equivalent combination of education and experience. ? Lab based Cytotechnologist or Histotechnologist experience. ? ASCP certification preferred but not required. ? Out of box thinker who can provide alternative ways to communicate a process ? Ability to effectively interact with a diverse group of people including various skill levels, degrees of seniority, personality, appreciation for the topic, and ego. ? Prior training and/or experience in automation focused ImmunoHisto Chemistry and In Situ Hybridization markets; including special stains and hematoxylin and Eosin preferred. Agilent Technologies, Inc. is an Equal Employment Opportunity and Affirmative Action employer. We value diversity at all levels. All individuals, regardless of personal characteristics, are encouraged to apply. All qualified applicants will receive consideration for employment without regard to sex, pregnancy, race, religion or religious creed, color, gender, gender identity, gender expression, national origin, ancestry, physical or mental disability, medical condition, genetic information, marital status, registered domestic partner status, age, sexual orientation, military or veteran status, protected veteran status, or any other basis protected by federal, state, local law, ordinance, or regulation and will not be discriminated against on these bases. For more information about equal employment opportunity protections, please view the ?EEO is the Law? poster available here https://www.dol.gov/ofccp/regs/compliance/posters/pdf/eeopost.pdf Agilent Technologies, Inc., is committed to diversity in the workplace and strives to support candidates with disabilities. If you have a disability and need assistance with any part of the application or interview process or have questions about workplace accessibility, please contact +1-262-754-5030 (US and Canada only) or email job_posting at agilent.com. EOE AA M/F/Vet/Disability Company: Dako Business: Diagnostics and Genomics Group Job Category: Support / Service Job Sub-Category: Education Region: Americas Country or Area: United States State/Province: California Town/City: Santa Clara Shift: Day Job Job Type: Experienced Schedule: Full-time Travel Required: Occasional Click on the following link to apply and upload your CV: If you are asked where you heard about this position, reference "Histonet" https://www.agilent.apply2jobs.com/ProfExt/index.cfm?fuseaction=mExternal.showJob&RID=2066078&CurrentPage=1 From histo at pathlab.us Thu May 12 11:55:42 2016 From: histo at pathlab.us (Histology) Date: Thu, 12 May 2016 16:55:42 +0000 Subject: [Histonet] HT needed in Norfolk, VA Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD823AECAD@vdc01.domain.local> Hi all, I have a position open for Histo Tech with a private dermpath lab in Norfolk, VA. Must have HS/GED be ASCP certified as a HT or HTL. Experience needed in routine and IHC. Full time M-F with morning hours. Fax confidential resume to 757-664-9122 Attn: Administrator From DKnutson at primecare.org Fri May 13 12:59:29 2016 From: DKnutson at primecare.org (Knutson, Deanne) Date: Fri, 13 May 2016 12:59:29 -0500 Subject: [Histonet] Job Opening Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A4D59712C1@EXCHANGE2K7.staprimecare.org> We have an opening for a certified Pathology Assistant to join six pathologists in a thriving anatomic pathology practice in the Northern Plains. Please feel free to contact me or visit our website at www.st.alexius.org where more information and application can be found. We look forward to hearing from you! Deanne Knutson Supervisor Anatomic Pathology 900 East Broadway Avenue, Bismarck, ND 58501-4520 P 701-530-6730 I F 701-530-6735 dknutson at primecare.org st.alexius.org [X] "Let All Be Received as Christ." ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From wgray19 at sc.rr.com Fri May 13 16:56:50 2016 From: wgray19 at sc.rr.com (Wanda Shotsberger Gray) Date: Fri, 13 May 2016 17:56:50 -0400 Subject: [Histonet] International Certification Message-ID: <154ac1f40d0.27e6.145c840c2545cc8d226fb8e47258f83d@sc.rr.com> A co-worker passed the HTL exam yesterday (Way to go Dan) and wants to know if his spiffy new HTL certificate is good internationally. Does anyone know? Thank you, Wanda Shotsberger HT/HTL (ASCP) Charleston, SC Sent with AquaMail for Android http://www.aqua-mail.com From Nancy_Schmitt at pa-ucl.com Mon May 16 10:03:25 2016 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Mon, 16 May 2016 15:03:25 +0000 Subject: [Histonet] floor vibration Message-ID: Happy Monday! We are moving to a new space and part of our area is above the laundry - there is some vibration from there. Does anyone have any experience with this and could you please share how you accommodated this? Special flooring, pads, etc. Thank you much! Nancy Schmitt HT, MLT (ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From paula at excaliburpathology.com Mon May 16 10:29:35 2016 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Mon, 16 May 2016 15:29:35 +0000 (UTC) Subject: [Histonet] floor vibration In-Reply-To: References: Message-ID: <1798931453.3273285.1463412575143.JavaMail.yahoo@mail.yahoo.com> Refuse the space and ask to be moved somewhere else. Years ago our EM scopes were on the fourth floor and lines in photos taken could be seen from the vibration from people simply walking down the halls. Finally moved the scopes to the basement on a section of concrete cut out completely separate from the rest of the building.?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Nancy Schmitt via Histonet To: "'histonet at lists.utsouthwestern.edu'" Sent: Monday, May 16, 2016 10:03 AM Subject: [Histonet] floor vibration Happy Monday! We are moving to a new space and part of our area is above the laundry - there is some vibration from there.? Does anyone have any experience with this and could you please share how you accommodated this?? Special flooring, pads, etc. Thank you much! Nancy Schmitt HT, MLT (ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mdmurphy at alaska.edu Mon May 16 13:11:03 2016 From: mdmurphy at alaska.edu (Molly Murphy) Date: Mon, 16 May 2016 10:11:03 -0800 Subject: [Histonet] request for antibody info Message-ID: Hello! Does anyone have a recommendation for a polyclonal anti-rabies virus antibody? I'd like to use it on mouse tissue for immunohistochemistry. I have previously seen FITC-conjugated goat anti-Rabies used in IHC (from Chemicon), but this product does not appear to be available any more. Thanks for any info! Molly -- Molly Murphy DVM, Ph.D, Diplomate, ACVP Assistant Professor of Veterinary Pathology College of Natural Sciences & Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 From relia1 at earthlink.net Mon May 16 15:02:19 2016 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 16 May 2016 16:02:19 -0400 Subject: [Histonet] RELIA Hot Histology Job alert!! Exciting Opportunity in Austin, TX Message-ID: <002801d1afad$f91fd130$eb5f7390$@earthlink.net> Hi Histonetters! I hope this is the start of an amazing week. I have a hot job opportunity that I would like to share. Here?s the info: RELIA Hot Histology Job Alert!! Exciting Opportunity in Austin, TX RELIA Solutions has been engaged EXCLUSIVELY by a growing lab located in exciting Austin, TX to recruit an experienced certified histotech. You will be responsible for performing routine histology, cutting, embedding and staining. ASCP HT/HTL. and 1-2 years of histology experience is required. Strong Derm, High volume and/or Special Stains experience preferred. My client offers excellent compensation a generous shift differential, great benefits, relocation assistance and a great team to work with. My client is eager to speak with you and ready to hire!! For more information please contact Pam Barker at relia1 at earthlink.net or toll free at 866-607-3542 or on my cell at 407-353-5070. RELIA Solutions is the nation's ONLY recruiting firm specializing in the nationwide permanent placement of histology professionals. To sign up for our free histology careers bulletin please send an e-mail to relia1 at earthlink.net and include subscribe in the subject line. Remember how much fun you had in Austin at the NSH/SC? Wouldn?t you love to play and work there? Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From jaylundgren at gmail.com Mon May 16 15:18:12 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Mon, 16 May 2016 13:18:12 -0700 Subject: [Histonet] floor vibration In-Reply-To: <1798931453.3273285.1463412575143.JavaMail.yahoo@mail.yahoo.com> References: <1798931453.3273285.1463412575143.JavaMail.yahoo@mail.yahoo.com> Message-ID: Wouldn't the anti-vibration measures work better if placed under the laundry equipment? They sell super expensive air bearing tables for EM applications, but I doubt routine histology would merit such expense. Good luck, try to enjoy the good vibrations. On Mon, May 16, 2016 at 8:29 AM, Paula Keene Pierce via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Refuse the space and ask to be moved somewhere else. > Years ago our EM scopes were on the fourth floor and lines in photos taken > could be seen from the vibration from people simply walking down the halls. > Finally moved the scopes to the basement on a section of concrete cut out > completely separate from the rest of the building. Paula Keene Pierce, BS, > HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, > OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com > > From: Nancy Schmitt via Histonet > To: "'histonet at lists.utsouthwestern.edu'" < > histonet at lists.utsouthwestern.edu> > Sent: Monday, May 16, 2016 10:03 AM > Subject: [Histonet] floor vibration > > Happy Monday! > We are moving to a new space and part of our area is above the laundry - > there is some vibration from there. Does anyone have any experience with > this and could you please share how you accommodated this? Special > flooring, pads, etc. > Thank you much! > > Nancy Schmitt HT, MLT (ASCP) > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From GKeyser at uwhealth.org Mon May 16 15:25:23 2016 From: GKeyser at uwhealth.org (Keyser Gerald T) Date: Mon, 16 May 2016 20:25:23 +0000 Subject: [Histonet] floor vibration In-Reply-To: References: Message-ID: <5226C88C65EBFF4BAD552D68DC6E8FFE1AFB8C37@UWHC-MBX12.uwhis.hosp.wisc.edu> I can think of two things. First, relentlessly make fun of your building planner for putting a histology lab underneath a laundry. This is a mistake worthy of pointing and laughing. Second, there are isolation tables and platforms. That's probably the first thing I would try. http://www.taab.co.uk/pdf-details/305_taab_products_1402580607.pdf Gerry -----Original Message----- From: Nancy Schmitt via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 16, 2016 10:03 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] floor vibration Happy Monday! We are moving to a new space and part of our area is above the laundry - there is some vibration from there. Does anyone have any experience with this and could you please share how you accommodated this? Special flooring, pads, etc. Thank you much! Nancy Schmitt HT, MLT (ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JRobinson at pathology-associates.com Mon May 16 17:07:43 2016 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Mon, 16 May 2016 22:07:43 +0000 Subject: [Histonet] floor vibration In-Reply-To: <5226C88C65EBFF4BAD552D68DC6E8FFE1AFB8C37@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <5226C88C65EBFF4BAD552D68DC6E8FFE1AFB8C37@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C90A20396@PAEXCH1.PathologyAssociates.local> An additional word of caution about placing the EM Lab under ANY kind of potential water leak. Tim Morken and I can attest to that! They installed a new CT scanner in Radiology directly above the EM Lab. They broke some sort of water pipe during the installation and it leaked so much that part of the ceiling collapsed onto the scope! They didn't bother checking to see if there was any damage on the next floor down- they just left. Tim had a huge surprise waiting for him the next morning. We always had to cover the scope with a sheet of plastic at night after that in case they installed something else. Jeff Robinson, Senior Histotechnologist, EM Tech (emeritus), Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Keyser Gerald T via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 16, 2016 1:25 PM To: 'Nancy Schmitt'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] floor vibration I can think of two things. First, relentlessly make fun of your building planner for putting a histology lab underneath a laundry. This is a mistake worthy of pointing and laughing. Second, there are isolation tables and platforms. That's probably the first thing I would try. http://www.taab.co.uk/pdf-details/305_taab_products_1402580607.pdf Gerry -----Original Message----- From: Nancy Schmitt via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 16, 2016 10:03 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] floor vibration Happy Monday! We are moving to a new space and part of our area is above the laundry - there is some vibration from there. Does anyone have any experience with this and could you please share how you accommodated this? Special flooring, pads, etc. Thank you much! Nancy Schmitt HT, MLT (ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From b-frederick at northwestern.edu Tue May 17 07:11:26 2016 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Tue, 17 May 2016 12:11:26 +0000 Subject: [Histonet] floor vibration In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C90A20396@PAEXCH1.PathologyAssociates.local> References: <5226C88C65EBFF4BAD552D68DC6E8FFE1AFB8C37@UWHC-MBX12.uwhis.hosp.wisc.edu> <204A03EB5A7F0A4BB1EEDD52A963829C90A20396@PAEXCH1.PathologyAssociates.local> Message-ID: <82bc5fc54f9f4b5bbbdd5afadf9639be@evcspmbx03.ads.northwestern.edu> We have issues as HVAC is above us! Sometimes at the switch over from heat to air and vice versa the facilities personnel don't check the drains and we get a flood. Came in one morning to hear dripping and we were going to a self -eval that day.! Most of the water was in my garbage can (Thankfully) Just missed my PC and the TMA arrayer. Got part of the embedding unit (it was a big leak). Facilities had the wet/dry vac up in the ceiling to get rid of the water. Had to have a good chunk of ceiling replaced and repaired after that one. There was even water in the light fixtures! Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu -----Original Message----- From: Jeffrey Robinson via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 16, 2016 5:08 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] floor vibration An additional word of caution about placing the EM Lab under ANY kind of potential water leak. Tim Morken and I can attest to that! They installed a new CT scanner in Radiology directly above the EM Lab. They broke some sort of water pipe during the installation and it leaked so much that part of the ceiling collapsed onto the scope! They didn't bother checking to see if there was any damage on the next floor down- they just left. Tim had a huge surprise waiting for him the next morning. We always had to cover the scope with a sheet of plastic at night after that in case they installed something else. Jeff Robinson, Senior Histotechnologist, EM Tech (emeritus), Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Keyser Gerald T via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 16, 2016 1:25 PM To: 'Nancy Schmitt'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] floor vibration I can think of two things. First, relentlessly make fun of your building planner for putting a histology lab underneath a laundry. This is a mistake worthy of pointing and laughing. Second, there are isolation tables and platforms. That's probably the first thing I would try. http://www.taab.co.uk/pdf-details/305_taab_products_1402580607.pdf Gerry -----Original Message----- From: Nancy Schmitt via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 16, 2016 10:03 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] floor vibration Happy Monday! We are moving to a new space and part of our area is above the laundry - there is some vibration from there. Does anyone have any experience with this and could you please share how you accommodated this? Special flooring, pads, etc. Thank you much! Nancy Schmitt HT, MLT (ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Tue May 17 08:35:15 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 17 May 2016 13:35:15 +0000 Subject: [Histonet] vibrations Message-ID: <48E053DDF6CE074DB6A7414BA05403F8073653@HRHEX02-HOS.holyredeemer.local> With experience and certification in both, I think that problems from ambient vibrations are much less severe in Histology than EM. There are special vibration dampening worktables, often used for EM, that would eliminate the problem for cutting stations, or any other sensitive equipment, and still allow you to use the space. I hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Monday, May 16, 2016 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 150, Issue 18 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. floor vibration (Nancy Schmitt) 2. Re: floor vibration (Paula Keene Pierce) ---------------------------------------------------------------------- Message: 1 Date: Mon, 16 May 2016 15:03:25 +0000 From: Nancy Schmitt To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] floor vibration Message-ID: Content-Type: text/plain; charset="us-ascii" Happy Monday! We are moving to a new space and part of our area is above the laundry - there is some vibration from there. Does anyone have any experience with this and could you please share how you accommodated this? Special flooring, pads, etc. Thank you much! Nancy Schmitt HT, MLT (ASCP) Message: 2 Date: Mon, 16 May 2016 15:29:35 +0000 (UTC) From: Paula Keene Pierce To: Nancy Schmitt , Histonet Subject: Re: [Histonet] floor vibration Message-ID: <1798931453.3273285.1463412575143.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Refuse the space and ask to be moved somewhere else. Years ago our EM scopes were on the fourth floor and lines in photos taken could be seen from the vibration from people simply walking down the halls. Finally moved the scopes to the basement on a section of concrete cut out completely separate from the rest of the building.?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Nancy Schmitt via Histonet To: "'histonet at lists.utsouthwestern.edu'" Sent: Monday, May 16, 2016 10:03 AM Subject: [Histonet] floor vibration Happy Monday! We are moving to a new space and part of our area is above the laundry - there is some vibration from there.? Does anyone have any experience with this and could you please share how you accommodated this?? Special flooring, pads, etc. Thank you much! Nancy Schmitt HT, MLT (ASCP) From rjbuesa at yahoo.com Tue May 17 08:58:40 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 17 May 2016 13:58:40 +0000 (UTC) Subject: [Histonet] vibrations In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8073653@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8073653@HRHEX02-HOS.holyredeemer.local> Message-ID: <1200800802.3367135.1463493520313.JavaMail.yahoo@mail.yahoo.com> Photomicrography could be affected at high resolution (immersion oil objectives) but probably could be eliminated if the microscope table is isolated from the floor with some vibration damping device.Ren? On Tuesday, May 17, 2016 9:52 AM, Terri Braud via Histonet wrote: With experience and certification in both, I think that problems from ambient vibrations are much less severe in Histology than EM.? There are special vibration dampening worktables, often used for EM, that would eliminate the problem for cutting stations, or any other sensitive equipment, and still allow you to use the space.? I hope this helps.? Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Monday, May 16, 2016 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 150, Issue 18 Send Histonet mailing list submissions to ??? histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. floor vibration (Nancy Schmitt) ? 2. Re: floor vibration (Paula Keene Pierce) ---------------------------------------------------------------------- Message: 1 Date: Mon, 16 May 2016 15:03:25 +0000 From: Nancy Schmitt To: "'histonet at lists.utsouthwestern.edu'" ??? Subject: [Histonet] floor vibration Message-ID: Content-Type: text/plain; charset="us-ascii" Happy Monday! We are moving to a new space and part of our area is above the laundry - there is some vibration from there.? Does anyone have any experience with this and could you please share how you accommodated this?? Special flooring, pads, etc. Thank you much! Nancy Schmitt HT, MLT (ASCP) Message: 2 Date: Mon, 16 May 2016 15:29:35 +0000 (UTC) From: Paula Keene Pierce To: Nancy Schmitt , ??? Histonet ??? Subject: Re: [Histonet] floor vibration Message-ID: ??? <1798931453.3273285.1463412575143.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Refuse the space and ask to be moved somewhere else. Years ago our EM scopes were on the fourth floor and lines in photos taken could be seen from the vibration from people simply walking down the halls. Finally moved the scopes to the basement on a section of concrete cut out completely separate from the rest of the building.?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com ? ? ? From: Nancy Schmitt via Histonet To: "'histonet at lists.utsouthwestern.edu'" Sent: Monday, May 16, 2016 10:03 AM Subject: [Histonet] floor vibration ? Happy Monday! We are moving to a new space and part of our area is above the laundry - there is some vibration from there.? Does anyone have any experience with this and could you please share how you accommodated this?? Special flooring, pads, etc. Thank you much! Nancy Schmitt HT, MLT (ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From clmcmah at clemson.edu Tue May 17 09:19:12 2016 From: clmcmah at clemson.edu (Chad L McMahan) Date: Tue, 17 May 2016 14:19:12 +0000 Subject: [Histonet] South Carolina announces annual SCSHT Histo-Expo Message-ID: To all Histonetters and colleagues of the profession: SCSHT is pleased to announce our annual fall South Carolina Society of Histotechnology Histo-Expo. Our event will be hosted at the Litchfield Beach and Golf Resort, Pawleys Island, South Carolina. The event will kick off on November 4-6, 2016. Throughout the year and the month of November we offer thanks for various reasons. During our fall event we are offering gratitude and thanks as we celebrate another great year with dear colleagues, vendors, and allied professionals across our region! Please mark your calendars and let me know if you would like to attend by phone, text, or email. Event details to follow. Our tentative schedule consists of speakers with lots of histology tenure, vendor fair with exhibit hall demos, career day, and generous southern hospitality! We look forward to your attendance and continued support. I will post details of speaker/workshop schedule, venue/lodging pricing, and additional details of event. Please take every opportunity to support your local society. Best regards, Chad McMahan, SCSHT President, Education Coordinator, Meeting Manager Chad L. McMahan, MBA, HT (ASCP)cm Clemson Bioengineering Biomaterials/Histology Lab Manager BioE Research Safety Liaison Clemson University, 104 Rhodes Hall, Clemson, SC 29634 CUBEInC, 200 Patewood Drive, Bldg C, Ste. 400, Greenville, 29615 864-656-5553 Clemson Office 864-284-6711 CUBEInC Office 864-656-4466 Fax 864-541-1340 Cell clmcmah at clemson.edu From Ryan.Roy at va.gov Tue May 17 11:55:09 2016 From: Ryan.Roy at va.gov (Roy, Ryan) Date: Tue, 17 May 2016 12:55:09 -0400 Subject: [Histonet] [EXTERNAL] In-Reply-To: References: Message-ID: <15F883394EAB744E99E1C7E1B9873049018C4FD9116B@R04BYNMSGB1.r04.med.va.gov> Hello, I wish you had contacted me a few months back. Management moved a tech over internally here. I will put you in contact with White River Junction VAMC . They may have a postion open. Also, I would check University of Vermont Med center;, Brattleboro Memorial hospital, Dartmouth Medical Center, Catholic Medical Center, Manchester. I know people at all these locations, often times a latent position (everyone knows about it ) won't post until a certain period so you wouldn' t know its there without asking. That said, Ill ask around. Ryan Roy HTL (ASCP) Histology Lab Manchester VA 718 Symth Rd Manchester NH 03104 (603) 624-4366 ex 6640 Disclosure: The content of this email does not reflect the policies, views or opinions of the VA. From: Kaylie Grenier [mailto:kgrenier2 at Student.Goodwin.edu] Sent: Tuesday, May 17, 2016 11:42 AM To: Roy, Ryan Subject: [EXTERNAL] Good morning, My name is Kaylie Grenier, I am a histology student at Goodwin College. Kelli Goodkowsky gave me your email address so I can get in contact with you. I am in my final semester of the program and I am hoping to relocate to New Hampshire at the end of August and am planning on taking my HT certification as soon as I am eligible. I was just wondering if you knew of any potential positions up in NH or if you knew of anyone I could get in contact with. I will be moving to the New London, NH area. Thanks! -Kaylie Grenier From jclark at pcnm.com Tue May 17 14:10:58 2016 From: jclark at pcnm.com (Joanne Clark) Date: Tue, 17 May 2016 19:10:58 +0000 Subject: [Histonet] JACHO Message-ID: <7A7BDD92B984E847A7E71BC9C00A66D31272AF92@S11MAILD034N2.sh11.lan> Hi Histonetters I have a question for those of you who have JACHO accreditation. Part 5 of QSA.13.04.01 states that when a nonpathologist performs gross analysis under the supervision of a qualified pathologist, that the individuals work is reviewed and documented within 24 hours. My question is how are you confirming and documenting this for specimens grossed in on Friday by the grossing tech that will not be read by the pathologist until Monday? Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From MMargiotta at bmhmc.org Wed May 18 10:23:51 2016 From: MMargiotta at bmhmc.org (Margiotta-Watz, Michele) Date: Wed, 18 May 2016 15:23:51 +0000 Subject: [Histonet] open position Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC6DA29754@BMH-EXCHANGE-01.BMHMC.ORG> Hi All, We have a full-time Histotechnologist position available at our community hospital on eastern Long Island. It is Monday thru Friday and no holidays. We are looking for someone to work either 4am-12 or 5am -1. If you are interested or know someone who might be interested, please e-mail or call. Thanks! Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Road Patchogue, NY 11772 631-654-7192 mmargiotta at bmhmc.org DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From carol.wilson at ricerca.com Wed May 18 11:30:30 2016 From: carol.wilson at ricerca.com (Wilson, Carol) Date: Wed, 18 May 2016 16:30:30 +0000 Subject: [Histonet] Processing Canine Nasal Passages Message-ID: Good Afternoon, I am looking for information on collection (ie. Upper jaw to eye socket?), decalcification(approximate timing, tips?), trimming (any tips/suggestions) and processing (without mega cassettes if possible) canine FFPE tissues. If someone can please give me some suggestions or ideas I would appreciate it. Or direction to an appropriate article? Thanks in advance. Carol Carol Wilson, HT(ASCP) Associate Scientist III Team Leader/Histopathology Ricerca Biosciences, LLC From CWaitts at Centrastate.com Wed May 18 12:43:36 2016 From: CWaitts at Centrastate.com (Waitts, Celeste) Date: Wed, 18 May 2016 17:43:36 +0000 Subject: [Histonet] Histonet Digest, Vol 150, Issue 20 In-Reply-To: References: Message-ID: Okay can you send to them? -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Wednesday, May 18, 2016 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 150, Issue 20 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. JACHO (Joanne Clark) 2. open position (Margiotta-Watz, Michele) 3. Processing Canine Nasal Passages (Wilson, Carol) ---------------------------------------------------------------------- Message: 1 Date: Tue, 17 May 2016 19:10:58 +0000 From: Joanne Clark To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] JACHO Message-ID: <7A7BDD92B984E847A7E71BC9C00A66D31272AF92 at S11MAILD034N2.sh11.lan> Content-Type: text/plain; charset="us-ascii" Hi Histonetters I have a question for those of you who have JACHO accreditation. Part 5 of QSA.13.04.01 states that when a nonpathologist performs gross analysis under the supervision of a qualified pathologist, that the individuals work is reviewed and documented within 24 hours. My question is how are you confirming and documenting this for specimens grossed in on Friday by the grossing tech that will not be read by the pathologist until Monday? Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Message: 2 Date: Wed, 18 May 2016 15:23:51 +0000 From: "Margiotta-Watz, Michele" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] open position Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC6DA29754 at BMH-EXCHANGE-01.BMHMC.ORG> Content-Type: text/plain; charset="us-ascii" Hi All, We have a full-time Histotechnologist position available at our community hospital on eastern Long Island. It is Monday thru Friday and no holidays. We are looking for someone to work either 4am-12 or 5am -1. If you are interested or know someone who might be interested, please e-mail or call. Thanks! Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Road Patchogue, NY 11772 631-654-7192 mmargiotta at bmhmc.org DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. ------------------------------ Message: 3 Date: Wed, 18 May 2016 16:30:30 +0000 From: "Wilson, Carol" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Processing Canine Nasal Passages Message-ID: Content-Type: text/plain; charset="us-ascii" Good Afternoon, I am looking for information on collection (ie. Upper jaw to eye socket?), decalcification(approximate timing, tips?), trimming (any tips/suggestions) and processing (without mega cassettes if possible) canine FFPE tissues. If someone can please give me some suggestions or ideas I would appreciate it. Or direction to an appropriate article? Thanks in advance. Carol Carol Wilson, HT(ASCP) Associate Scientist III Team Leader/Histopathology Ricerca Biosciences, LLC ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 150, Issue 20 ***************************************** From melissa at alliedsearchpartners.com Wed May 18 13:59:47 2016 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Wed, 18 May 2016 18:59:47 +0000 Subject: [Histonet] Temporary 2 Month Position in Aurora, CO- Message-ID: Hello All, I have a client who needs a temporary Histotech as a fill-in Mid August through to Mid-October. The schedule is Tuesday-Friday 6am-7pm and is in Aurora, CO. Please let me know if you would like details. We are looking locally for now. :) P.S. If Florida folks will be at the Florida Histotech Society Meeting this weekend please stop by our exhibit booth to say Hi. Looking forward to it! Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 From nto at stowers.org Wed May 18 14:29:58 2016 From: nto at stowers.org (Thomas, Nancy) Date: Wed, 18 May 2016 19:29:58 +0000 Subject: [Histonet] shrinking mouse eyes Message-ID: Dear experts, We often get a request to embed and section mouse eyes in GMA. Currently we have been working with samples from 1 month old mice. The big problem we need to solve is that the shape of the eyes is fine up until we embed them. I'm sure the heat from the exothermic reaction is causing the eyes to sink inward, losing their round shape, but what can prevent this? We use the Immuno-bed kit which sets up fine and sections well. Is there something more I can do during dehydration and infiltration steps? We have experimented with longer dehydration times, but haven't seen much difference. The eyes were fixed in Davidson's for 24 hr. Would longer fixation help? Thank you in advance for all advice, Nancy Thomas Senior Lab Manager, Histology Core Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 From liz at premierlab.com Wed May 18 16:30:01 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Wed, 18 May 2016 15:30:01 -0600 Subject: [Histonet] shrinking mouse eyes In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02BEC2AD5FC7@SBS2K8.premierlab.local> Nancy I cannot comment on the plastic embedding but this also happens with paraffin embedded samples. We trim off one of the edges on all mouse and rat eye samples and that eliminates that problem with paraffin embedded samples. I can send pictures of a rat eye that has been trimmed. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Thomas, Nancy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, May 18, 2016 1:30 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] shrinking mouse eyes Dear experts, We often get a request to embed and section mouse eyes in GMA. Currently we have been working with samples from 1 month old mice. The big problem we need to solve is that the shape of the eyes is fine up until we embed them. I'm sure the heat from the exothermic reaction is causing the eyes to sink inward, losing their round shape, but what can prevent this? We use the Immuno-bed kit which sets up fine and sections well. Is there something more I can do during dehydration and infiltration steps? We have experimented with longer dehydration times, but haven't seen much difference. The eyes were fixed in Davidson's for 24 hr. Would longer fixation help? Thank you in advance for all advice, Nancy Thomas Senior Lab Manager, Histology Core Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 at cdc.gov Thu May 19 05:39:35 2016 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Thu, 19 May 2016 10:39:35 +0000 Subject: [Histonet] processing cross-contamination Message-ID: <3B2CD438E1628A41BD687E98B963B781687C4D0B@EMBX-CLFT4.cdc.gov> Morning everyone, I would like to know how many labs experience issues where tissue from a typical, sealed cassette is lost in the processor and ends up inside another cassette. Really. Thanks, Jeanine Sanders CDC Atlanta From tbraud at holyredeemer.com Thu May 19 13:06:09 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 19 May 2016 18:06:09 +0000 Subject: [Histonet] processing cross contaminations Message-ID: <48E053DDF6CE074DB6A7414BA05403F8075099@HRHEX02-HOS.holyredeemer.local> Hi Jeanine - I can't remember the last time that I had any cross contamination in the tissue processor. If the tissue is properly grossed without contamination, secured within a cassette (we use blue pads for tiny hard tissue; lens paper for fragile biopsies, such as liver; and nylon mesh bags for fragmented specimens) then there should be no cross contamination within the processor. Possible block contamination sites are at gross, and at embedding (we only open one cassette at a time, and wipe forceps with gauze in between cassettes). Slides can be contaminated with "floaters" from the waterbath during cutting, but a CAP study about 10 years ago found that most cross contamination occurs during grossing. We actively monitor for contamination and have not had any in 15+ years. I hope this information helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Today's Topics: 5. processing cross-contamination (Sanders, Jeanine (CDC/OID/NCEZID)) Message: 5 Date: Thu, 19 May 2016 10:39:35 +0000 From: "Sanders, Jeanine (CDC/OID/NCEZID)" Subject: [Histonet] processing cross-contamination Morning everyone, I would like to know how many labs experience issues where tissue from a typical, sealed cassette is lost in the processor and ends up inside another cassette. Really. Thanks, Jeanine Sanders CDC Atlanta From jmoreira at sidra.org Fri May 20 01:54:41 2016 From: jmoreira at sidra.org (Joana Moreira) Date: Fri, 20 May 2016 06:54:41 +0000 Subject: [Histonet] =?windows-1252?q?Leica_IP_C_Cassette_Printer_Integrati?= =?windows-1252?q?on_with_Cerner_Millennium_=96_Pathnet_AP?= Message-ID: Hi everyone! I am seeking help from someone that has been or is currently going through the process of interfacing the Leica IP C cassette printer with the AP Module of Cerner Millenium. I know we need a Nice label software for the interface to happen more swiftly. There's some other questions I need to have clarified given my department requirements for cassette printing but we keep hitting a brick wall with Cerner. If you have been or currently going through the same and are willing to share your experience please send me a personal message. Many Thanks in advance, Joana Joana Moreira Supervisor ? Anatomical Pathology and Morgue Department of Pathology Sidra Medical and Research Center Hospital, 2nd Mezzanine Floor, Office H2M-24143 PO Box 26999, Doha, Qatar D: +974-4403-2997 | M: +974-3398-1923 jmoreira at sidra.org | www.sidra.org Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. From relia1 at earthlink.net Fri May 20 10:29:51 2016 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 20 May 2016 11:29:51 -0400 Subject: [Histonet] RELIA Hot Job Alert 5-120-2016 Exciting and Immediate Opportunities in VA, TX, TN, CA, AZ, AL, AR, KS and CO Message-ID: <00cb01d1b2ac$74222990$5c667cb0$@earthlink.net> Hi Histonetters! I hope you had a great week and are looking forward to a fantastic weekend! I wanted to send a quick note to tell you about the positions that I am working on and am most excited about. Why am I excited about these positions? Because each of these clients was asked: "If I had a histotech for you today would you be ready to interview and hire?" Every One these clients said YES!!! All of these positions are full time and permanent!! My clients offer excellent compensation, benefits and in most cases either relocation or a sign-on bonus. The Leadership positions are located in Texas, Tennessee and Arkansas. The HT/HTL positions are located in: Texas Tennessee Virginia Arizona Arkansas Alabama Indiana California Colorado If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I place someone you refer You will earn a referral fee. I can be reached toll free at the office at 866-607-3542 or relia1 at earthlink.net or you can always catch me on cell via call or text at 407-353-5070. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From relia1 at earthlink.net Mon May 23 08:05:48 2016 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 23 May 2016 09:05:48 -0400 Subject: [Histonet] Histonet for Med Techs Message-ID: <014801d1b4f3$d3541290$79fc37b0$@earthlink.net> Hi Histonetters! I hope everyone is looking forward to a great week and a fantastic Memorial Day Weekend. I am hoping someone can help me with this. I would like to know if anyone is aware of a forum similar to histonet but for med techs? (Besides Medlab out of the University of Buffalo). Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From JMacDonald at mtsac.edu Mon May 23 08:29:41 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Mon, 23 May 2016 06:29:41 -0700 Subject: [Histonet] Histonet for Med Techs In-Reply-To: <014801d1b4f3$d3541290$79fc37b0$@earthlink.net> Message-ID: CLSEDUC at LIST.APSU.EDU This is a list serve for MT/MLT Educators. Sent from my iPhone > On May 23, 2016, at 6:09 AM, Pam Barker via Histonet wrote: > > Hi Histonetters! > I hope everyone is looking forward to a great week and a fantastic Memorial > Day Weekend. I am hoping someone can help me with this. > I would like to know if anyone is aware of a forum similar to histonet but > for med techs? (Besides Medlab out of the University of Buffalo). > > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > Pam M. Barker > > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1 at earthlink.net > www.facebook.com/PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fpearsa at clemson.edu Mon May 23 12:30:44 2016 From: fpearsa at clemson.edu (Frances Pearsall) Date: Mon, 23 May 2016 17:30:44 +0000 Subject: [Histonet] Gemini AS stainer Message-ID: We recently purchased a Gemini AS Stainer brand new. I am looking for any tips on cleaning the tubs of the Hematoxylin and Eosin stains from them? The manual says to use soapy water, but I'm still having 'dirty' tubs. Any suggestions would greatly appreciated. Thank you! Fran From SteveM at mcclainlab.com Mon May 23 13:26:46 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Mon, 23 May 2016 18:26:46 +0000 Subject: [Histonet] Histonet Digest, Vol 150, Issue 21 floaters In-Reply-To: References: Message-ID: <0547006B-DA06-4D5E-B69B-D1774EF68C1B@mcclainlab.com> Never seen that happen in 34 years of training and practice. Sounds like an inadvertent transfer by the grosser. But Floaters are so frequent that it behooves labs processing small biopsies to wrap each specimen to minimize or eliminate transfers. We have the embeddets clean the forceps w the wrappers to further minimize transfer. I have posted long messages previously, detailing methods. However tiny floaters can persist for months in the tissue processor pumps and bottles and lines. 20 years ago I inherited an old Fishermatic 160, previously used to process placentas. I saw placenta fragments in skin samples for at least 6 months. Steve Morning everyone, I would like to know how many labs experience issues where tissue from a typical, sealed cassette is lost in the processor and ends up inside another cassette. Really. Thanks, Jeanine Sanders CDC Atlanta Steve A. McClain, MD > On May 19, 2016, at 13:31, "histonet-request at lists.utsouthwestern.edu" wrote: > > Morning everyone, > > I would like to know how many labs experience issues where tissue from a typical, sealed cassette is lost in the processor and ends up inside another cassette. > > Really. > > Thanks, > Jeanine Sanders > CDC Atlanta From jblanchard at reliapath.com Mon May 23 14:37:34 2016 From: jblanchard at reliapath.com (Jeanne Blanchard) Date: Mon, 23 May 2016 19:37:34 +0000 Subject: [Histonet] Gemini AS stainer In-Reply-To: References: Message-ID: We soak ours in a bleach solution. Before dumping out the bleach solution, we scrub the tubs. Rinse with water VERY well and let dry. Jeanne Blanchard, CT (ASCP)CM -----Original Message----- From: Frances Pearsall via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, May 23, 2016 12:31 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Gemini AS stainer We recently purchased a Gemini AS Stainer brand new. I am looking for any tips on cleaning the tubs of the Hematoxylin and Eosin stains from them? The manual says to use soapy water, but I'm still having 'dirty' tubs. Any suggestions would greatly appreciated. Thank you! Fran _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun at hhchealth.org Mon May 23 15:50:43 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 23 May 2016 20:50:43 +0000 Subject: [Histonet] Date-of-Service for Consults Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B0D23F9@HHCEXCHMB03.hhcsystem.org> I have always maintained that the "Date-of-Service" for a pathology consult is the date that the pathology consult is initiated or requested. Is that correct? Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From joyce.weems at emoryhealthcare.org Mon May 23 16:33:18 2016 From: joyce.weems at emoryhealthcare.org (Weems, Joyce K.) Date: Mon, 23 May 2016 21:33:18 +0000 Subject: [Histonet] Sakura Cassette Printer Message-ID: Hello Histo Peeps! Who has the new Sakura Cassette Printer? Would you share your experience with me? TIA - j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From A.Chan at victorchang.edu.au Mon May 23 22:31:26 2016 From: A.Chan at victorchang.edu.au (Andrea Chan) Date: Tue, 24 May 2016 03:31:26 +0000 Subject: [Histonet] whipf's polychrome stain Message-ID: Hi, Does anyone have a working protocol for Whipf's polychrome stain? From victor_tobias at comcast.net Mon May 23 22:51:18 2016 From: victor_tobias at comcast.net (Victor) Date: Mon, 23 May 2016 20:51:18 -0700 Subject: [Histonet] Date-of-Service for Consults In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E6B0D23F9@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E6B0D23F9@HHCEXCHMB03.hhcsystem.org> Message-ID: That is what go by. Victor Sent from Mail for Windows 10 From: Cartun, Richard via Histonet Sent: Monday, May 23, 2016 1:51 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Date-of-Service for Consults I have always maintained that the "Date-of-Service" for a pathology consult is the date that the pathology consult is initiated or requested. Is that correct? Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From raestask at grics.net Tue May 24 07:01:04 2016 From: raestask at grics.net (raestask at grics.net) Date: Tue, 24 May 2016 08:01:04 -0400 (EDT) Subject: [Histonet] Antibody MyD88 Message-ID: <925561068.81073399.1464091264719.JavaMail.root@grics.net> Does anyone have a protocol using the Ventana Ultra for this antibody? Thanks in advance. Rae Staskiewicz UnityPoint Health-Methodist/Proctor From amurvosh at advancederm.net Tue May 24 09:28:18 2016 From: amurvosh at advancederm.net (Anne Murvosh) Date: Tue, 24 May 2016 14:28:18 +0000 Subject: [Histonet] Hoods Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7B0AD3B@Exchange.Advancederm.net> We had an inspection from the fire department recently and they said we need to have the exhaust from the fume hood checked annually. Does anyone know who does that? The people that do our P.M. on our machines say they don't, so who would? Thanks Anne From james.fortune at unitypoint.org Tue May 24 09:39:16 2016 From: james.fortune at unitypoint.org (Fortune, James A.) Date: Tue, 24 May 2016 14:39:16 +0000 Subject: [Histonet] GHS vs. NFPA labeling Message-ID: So I have a question about the labelling of chemicals. I understand that new liquid chemicals must have the GHS labels, and I noticed some of our dry chemicals also have the new GHS labelling. What about old dyes and other dry chemicals that are still good but do not have the GHS labels but have the old NFPA labels? As most of you know dyes are expensive and most are very old and still work great. So I want to clarify this before we go crazy and start trashing dry chemicals and dyes. Any input would be greatly appreciated. Andy Fortune Tech Specialist Meriter Laboratories Madison, WIi This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. SSSS 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From Bonnie.Whitaker at osumc.edu Tue May 24 09:42:04 2016 From: Bonnie.Whitaker at osumc.edu (Whitaker, Bonnie) Date: Tue, 24 May 2016 10:42:04 -0400 Subject: [Histonet] Hoods In-Reply-To: <22BDD9AABC13E24E95D1CF064B75C4B7B0AD3B@Exchange.Advancederm.net> References: <22BDD9AABC13E24E95D1CF064B75C4B7B0AD3B@Exchange.Advancederm.net> Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670E2D5886BE@EXMBOX-VP05.OSUMC.EDU> Check with you facilities management staff, if you are in a hospital or university setting. If you are in a private lab, contact whomever you contract for HVAC issues. If they can't provide the service, they can likely recommend someone. Good luck! Bonnie Sent with Good (www.good.com) Bonnie -----Original Message----- From: Anne Murvosh via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 24, 2016 10:30 AM Eastern Standard Time To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Hoods We had an inspection from the fire department recently and they said we need to have the exhaust from the fume hood checked annually. Does anyone know who does that? The people that do our P.M. on our machines say they don't, so who would? Thanks Anne _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=CwICAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=8uaTGxsVXGXQ_Eic2ooqHOzOw1PNx_urWO9PnfaiX3w&s=Fv0TJRQt8GOIrZQ8M1hanS0GcV1tXAFPsiMEhXPke68&e= From jblanchard at reliapath.com Tue May 24 11:17:57 2016 From: jblanchard at reliapath.com (Jeanne Blanchard) Date: Tue, 24 May 2016 16:17:57 +0000 Subject: [Histonet] GHS vs. NFPA labeling In-Reply-To: References: Message-ID: I believe you can purchase the new GHS labels and just affix them to your reagents that don't already have them. Jeanne Blanchard, CT (ASCP)CM -----Original Message----- From: Fortune, James A. via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 24, 2016 9:39 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] GHS vs. NFPA labeling So I have a question about the labelling of chemicals. I understand that new liquid chemicals must have the GHS labels, and I noticed some of our dry chemicals also have the new GHS labelling. What about old dyes and other dry chemicals that are still good but do not have the GHS labels but have the old NFPA labels? As most of you know dyes are expensive and most are very old and still work great. So I want to clarify this before we go crazy and start trashing dry chemicals and dyes. Any input would be greatly appreciated. Andy Fortune Tech Specialist Meriter Laboratories Madison, WIi This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. SSSS 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Julia.Cates at AHSS.ORG Tue May 24 15:06:22 2016 From: Julia.Cates at AHSS.ORG (Cates, Julia) Date: Tue, 24 May 2016 20:06:22 +0000 Subject: [Histonet] GHS vs. NFPA labeling Message-ID: See the link below for a recent blog entry addressing this very question. I do not believe placing a new label on an original container is not permitted. I would transfer to a secondary container, if able, and label accordingly. http://danthelabsafetyman.tumblr.com/ Thanks, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Park Ridge Health Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. From Richard.Cartun at hhchealth.org Tue May 24 15:09:18 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 24 May 2016 20:09:18 +0000 Subject: [Histonet] Question - CoPath Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B0D26A5@HHCEXCHMB03.hhcsystem.org> We will be switching from Cerner CoPath to Sunrise CoPath in the near future (part of our Epic conversion). Is there anyone using Sunrise CoPath that uses a prostate diagram in their report to demonstrate the absence or presence of cancer in the different quadrants? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Richard.Cartun at hhchealth.org Tue May 24 15:35:35 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 24 May 2016 20:35:35 +0000 Subject: [Histonet] FW: Question - CoPath In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E6B0D26A5@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E6B0D26A5@HHCEXCHMB03.hhcsystem.org> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B0D26DE@HHCEXCHMB03.hhcsystem.org> Make that "Sunquest" CoPath ...... Richard -----Original Message----- From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 24, 2016 4:09 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Question - CoPath We will be switching from Cerner CoPath to Sunrise CoPath in the near future (part of our Epic conversion). Is there anyone using Sunrise CoPath that uses a prostate diagram in their report to demonstrate the absence or presence of cancer in the different quadrants? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From litepath2000 at yahoo.com Tue May 24 16:08:33 2016 From: litepath2000 at yahoo.com (NYSHisto) Date: Tue, 24 May 2016 21:08:33 +0000 (UTC) Subject: [Histonet] Employment Opportunity: Cold Spring Harbor Laboratories References: <1713763854.13297.1464124113639.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1713763854.13297.1464124113639.JavaMail.yahoo@mail.yahoo.com> Research Investigator/ Director, Tissue Imaging at the Cancer Centerof Cold Spring Harbor Laboratory???????Job SummaryThe Tissue ImagingTeam processes and generates all histology analysisfor CSHL?s basic and preclinical cancer research. The team processesanimal and human tissuehistology and develops state of the art histology visualization.We are seeking a highly motivatedresearch scientist to head the team and run automatedantibody applications.?Key Qualifications???????????Extensive experience with automated histologystaining in fieldsof medical, health or developmental biology.???????????Extensive experience with experimental design,development and execution of immunohistochemistry at core facilityor equivalent setting???????????Experience with administration and budgeting at a core facility???????????Experience with troubleshooting and maintaining automated histology equipment.?DescriptionKey responsibilities include: Lead a three person team dedicatedto histology services. Develop and streamline the team?s histology workflow from tissueembedding to digital archivingand sharing of virtual slides.Design, troubleshooting and optimization of all immunohistology staining using automated slide staining system. Responsibility for providing reliabledata for validation of research hypotheses. Close collaboration with variouscancer research groups.?EducationDegree: Ph.D., M.D. or D.V.M., Master?smay be considered given exceptional experience.Field of Study: Clinical,Pre-clinical medical research or developmental biology?Additional RequirementsExcellent communication and interpersonal skills. Strong analytical and bench problem-solving skills. Must thrive in fast-paced environment and keep abreast of relevantdevelopments in the field. Must be highlyorganized, and able to multitask. Demonstrated abilityto work as a key member of a team.?Please send your materials todtsang at cshl.edu?Cold SpringHarbor Laboratory is recognized internationally for its excellence in research and educational activities and support innovative research programs in cancer, neuroscience, plant biology, quantitative biology and genomics. Additional information about CSHL and the Watson Schoolcan be obtained at www.cshl.edu. ColdSpring Harbor Laboratory is an equal opportunity employer. From litepath2000 at yahoo.com Tue May 24 16:07:01 2016 From: litepath2000 at yahoo.com (NYSHisto) Date: Tue, 24 May 2016 21:07:01 +0000 (UTC) Subject: [Histonet] Employment Opportunity: Cold Spring Harbor Laboratories References: <701635614.32193.1464124021849.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <701635614.32193.1464124021849.JavaMail.yahoo@mail.yahoo.com> Technician, Tissue Imaging at the CancerCenter of Cold Spring HarborLaboratory?????Job SummaryThe Tissue ImagingTeam processes and generates all histology analysisfor CSHL?s basic and preclinical cancer research. The team processesanimal and human tissuehistology and develops state of the art tissue visualization for the center?sresearch teams.We are seeking a highly motivated research technician to provide histologyservices for scientific investigators.?Key Qualifications???????????Experience with histologystaining techniques or comparable techniques involving histochemistry???????????Experience with generating highly reproducible histologyresults.???????????Experience with troubleshooting and maintaining generalhistology equipment???DescriptionKey responsibilities include: Performance of routine histology tasks on humanand animal tissues. Executea streamlined histology workflow from tissueembedding to digital archiving and sharing of virtual slides.Evaluate the qualityof samples submittedand returned to researchers. Closecollaboration with variouscancer research groups. Scheduling, maintenance of sampleand billing records.Maintenance of equipment and instruments. Generallaboratory organization and ordering of supplies.?EducationDegree: Bachelor?s or Master?s in histology or comparable field. Experience as histo- technician. Should be ASCP certified as an H.T. or H.T.L.Field of Study: Clinical,Pre-clinical medical research or developmental biology.?Additional RequirementsExcellent communication and interpersonal skills. Bench problem-solving skills. Must thrive in fast-pacedenvironment. Must be highly organized,and able to multitask. Demonstrated abilityto work as a member of a team.?Please send your materials todtsang at cshl.edu?Cold SpringHarbor Laboratory is recognized internationally for its excellence in research and educational activities and support innovative research programs in cancer, neuroscience, plant biology, quantitative biology and genomics. Additional information about CSHL and the Watson Schoolcan be obtained at www.cshl.edu. ColdSpring Harbor Laboratory is an equal opportunity employer. From mtoole at dcol.net Tue May 24 17:08:51 2016 From: mtoole at dcol.net (Mike Toole) Date: Tue, 24 May 2016 17:08:51 -0500 Subject: [Histonet] Pap stain without xylene Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB33E1B2A3240@mail> Ren? and other Histonetters, After reading the paper: Buesa RJ, Peshkov, MV. Histology without xylene. Ann Diagn Pathol. 2009 Aug;13(4):246-56. Epub 2009 Feb 5. It appears that xylene in the final clearing steps is replaced with isopropanol and mineral oil as follows: ? 5:1 isopropanol to mineral oil 50?C ? 2:1 isopropanol to mineral oil 50?C ? Undiluted mineral oil 50?C ? Drying oven 5 minutes 60?C ? Coverslip Do you feel these clearing steps be applied to the pap stain in order to eliminate xylene? If so, can it be done at room temperature? Thanks, Mike Mike Toole, BS, CT(ASCP)CM From taylor at prometheushealthcare.com Wed May 25 08:35:03 2016 From: taylor at prometheushealthcare.com (Taylor Rinaldi) Date: Wed, 25 May 2016 09:35:03 -0400 Subject: [Histonet] **CALIFORNIA** Histotechnician Job Opportunity Message-ID: <0ea601d1b68a$3fd79490$bf86bdb0$@prometheushealthcare.com> Hello all! My name is Taylor Rinaldi, Recruiting Manager at Prometheus healthcare. My team and I specialize specifically in laboratory staffing and recruiting all over the United States for hospitals and reference laboratories. We just received a new order for a great opportunity with a well-known laboratory located in California. We are recruiting for a strong Histotechnician for the lab. This position is a fulltime, permanent opportunity. ASCP certification preferred but not required. Relocation assistance offered. If you may be interested in this opportunity or would like to hear about our nationwide histotech openings, please reach out to me for immediate consideration. Thank you in advance! Taylor Rinaldi Recruiting Manager Prometheus Healthcare Office 866-857-1434 Taylor at prometheushealthcare.com From rjbuesa at yahoo.com Wed May 25 08:57:43 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 25 May 2016 13:57:43 +0000 (UTC) Subject: [Histonet] Pap stain without xylene In-Reply-To: <31530E35E0BAB044B3B56B7FE5CF4EB33E1B2A3240@mail> References: <31530E35E0BAB044B3B56B7FE5CF4EB33E1B2A3240@mail> Message-ID: <551124950.344327.1464184663854.JavaMail.yahoo@mail.yahoo.com> Hi Mike:The steps you?desrcibe are wrong.After you finish staining your PAP smear, just wash them in the last ethanol ? oven dry at 60?C for 5 minutes or as required if the smear is too thick and when completely dried ? coverslip.This final drying has to take place at temperatures above room temp. because you have to be absolutely sure the smear (or stained tissue section)? is absolutely dried before applying the mounting medium ? coverslip.The steps you write about 2-propanol and mineral oil are those the tissues have to go through before paraffin infiltration.?Ren? On Tuesday, May 24, 2016 6:36 PM, Mike Toole via Histonet wrote: Ren? and other Histonetters, After reading the paper: Buesa RJ, Peshkov, MV. Histology without xylene. Ann Diagn Pathol. 2009 Aug;13(4):246-56. Epub 2009 Feb 5. It appears that xylene in the final clearing steps is replaced with isopropanol and mineral oil as follows: ?? ? ? ? 5:1? ? ? ? isopropanol to mineral oil? ? ? ? ? ? 50?C ?? ? ? ? 2:1? ? ? ? isopropanol to mineral oil? ? ? ? ? ? 50?C ?? ? ? ? Undiluted mineral oil? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 50?C ?? ? ? ? Drying oven 5 minutes? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 60?C ?? ? ? ? Coverslip Do you feel these clearing steps be applied to the pap stain in order to eliminate xylene?? If so, can it be done at room temperature? Thanks, Mike Mike Toole, BS, CT(ASCP)CM _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Julia.Cates at AHSS.ORG Wed May 25 12:09:49 2016 From: Julia.Cates at AHSS.ORG (Cates, Julia) Date: Wed, 25 May 2016 17:09:49 +0000 Subject: [Histonet] GHS vs. NFPA labeling Message-ID: Oops! I do not believe it is permitted:) I need to read more carefully before I send out emails!! See the link below for a recent blog entry addressing this very question. I do not believe placing a new label on an original container is not permitted. I would transfer to a secondary container, if able, and label accordingly. http://danthelabsafetyman.tumblr.com/ Thanks, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Park Ridge Health (828)650-8243| Fax: (828)209-5315 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. From j.benavides at eae.csic.es Wed May 25 12:39:55 2016 From: j.benavides at eae.csic.es (Julio Benavides) Date: Wed, 25 May 2016 19:39:55 +0200 Subject: [Histonet] Stamp staining in paraffin and smear samples In-Reply-To: <551124950.344327.1464184663854.JavaMail.yahoo@mail.yahoo.com> References: <31530E35E0BAB044B3B56B7FE5CF4EB33E1B2A3240@mail> <551124950.344327.1464184663854.JavaMail.yahoo@mail.yahoo.com> Message-ID: <20160525193955.Horde.7jUwZVWRhZ0I6pIKi_tMOw1@webmail.csic.es> Hi There, Is anyone using this technique to stain chlamydia in placental samples (working with sheep here)? I was looking for a working technique but it looks like it is not easy to find. Thanks for your thoughts! Julio Rene J Buesa via Histonet escribi?: > Hi Mike:The steps you?desrcibe are wrong.After you finish staining > your PAP smear, just wash them in the last ethanol ? oven dry at > 60?C for 5 minutes or as required if the smear is too thick and when > completely dried ? coverslip.This final drying has to take place at > temperatures above room temp. because you have to be absolutely sure > the smear (or stained tissue section)? is absolutely dried before > applying the mounting medium ? coverslip.The steps you write about > 2-propanol and mineral oil are those the tissues have to go through > before paraffin infiltration.?Ren? > > On Tuesday, May 24, 2016 6:36 PM, Mike Toole via Histonet > wrote: > > > Ren? and other Histonetters, > > > After reading the paper: > > > > Buesa RJ, Peshkov, MV. Histology without xylene. Ann Diagn Pathol. > 2009 Aug;13(4):246-56. Epub 2009 Feb 5. > > > > It appears that xylene in the final clearing steps is replaced with > isopropanol and mineral oil as follows: > > ?? ? ? ? 5:1? ? ? ? isopropanol to mineral oil? ? ? ? ? ? 50?C > > ?? ? ? ? 2:1? ? ? ? isopropanol to mineral oil? ? ? ? ? ? 50?C > > ?? ? ? ? Undiluted mineral oil? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 50?C > > ?? ? ? ? Drying oven 5 minutes? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 60?C > > ?? ? ? ? Coverslip > > > > Do you feel these clearing steps be applied to the pap stain in > order to eliminate xylene?? If so, can it be done at room temperature? > > Thanks, > Mike > > Mike Toole, BS, CT(ASCP)CM > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bethcoxx at gmail.com Wed May 25 12:55:48 2016 From: bethcoxx at gmail.com (Beth Cox) Date: Wed, 25 May 2016 13:55:48 -0400 Subject: [Histonet] Pap stain without xylene Message-ID: <0223127d-9bf6-89b6-238e-164b4006f94f@gmail.com> Mike, As an alternative for your discussion: I work on medical mission trips where I run Paps in a field setting. For the past 4 years, we have not had access to xylene, and we have done the following: 1. Remove slides from the last alcohol after staining 2. Allow to air dry completely (generally only takes a few minutes) 3. Use standard mounting media to coverslip them dry. This works beautifully. Microscopically they look the same as xylene cleared slides. The only caveat is that they must dry completely. -- *BETH COX, HTL/SCT(ASCP)QIHC* AP Consultant | Pathology Solutions, Inc | (810) 240-2190 | bethcoxx at gmail.com ------------------------------ Message: 6 Date: Tue, 24 May 2016 17:08:51 -0500 From: Mike Toole To:"histonet at lists.utsouthwestern.edu" Subject: [Histonet] Pap stain without xylene Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB33E1B2A3240 at mail> Content-Type: text/plain; charset="iso-8859-1" Ren? and other Histonetters, After reading the paper: Buesa RJ, Peshkov, MV. Histology without xylene. Ann Diagn Pathol. 2009 Aug;13(4):246-56. Epub 2009 Feb 5. It appears that xylene in the final clearing steps is replaced with isopropanol and mineral oil as follows: ? 5:1 isopropanol to mineral oil 50?C ? 2:1 isopropanol to mineral oil 50?C ? Undiluted mineral oil 50?C ? Drying oven 5 minutes 60?C ? Coverslip Do you feel these clearing steps be applied to the pap stain in order to eliminate xylene? If so, can it be done at room temperature? Thanks, Mike Mike Toole, BS, CT(ASCP)CM From tbraud at holyredeemer.com Wed May 25 12:55:30 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 25 May 2016 17:55:30 +0000 Subject: [Histonet] SQ CoPath prostate diagram Message-ID: <48E053DDF6CE074DB6A7414BA05403F8076F9F@HRHEX02-HOS.holyredeemer.local> I don't have a diagram, but there is a really good SQ CoPath list serve where you might find your answer. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 2. Question - CoPath (Cartun, Richard) 3. FW: Question - CoPath (Cartun, Richard) From mtoole at dcol.net Wed May 25 14:07:20 2016 From: mtoole at dcol.net (Mike Toole) Date: Wed, 25 May 2016 14:07:20 -0500 Subject: [Histonet] Pap stain without xylene Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB33E1B2A344D@mail> Thank you Beth, It?s good to know that someone else had tried this and had very good results. The method you suggest is very much in line with the recommendations from Ren?. He did recommend using a drying oven at 60?C to help ensure complete removal of any water or alcohol. And, that absolute dryness was a requirement for coverslipping without artifact such as the appearance of sand like grains or cornflakes. Just to reiterate, was the field method performed at ambient temperature without the aid of a drying oven? And, just a thought, I suppose if the lack of electricity was an issue in a field setting, that perhaps a solar oven made with plywood and glass could be used to elevate temperature for drying. Do you know if altering the method for final clearing would require validation? Mike From: Beth Cox [mailto:bethcoxx at gmail.com] Sent: Wednesday, May 25, 2016 12:56 PM To: Mike Toole; Histonet Subject: [EXTERNAL] Re: Pap stain without xylene Mike, As an alternative for your discussion: I work on medical mission trips where I run Paps in a field setting. For the past 4 years, we have not had access to xylene, and we have done the following: 1. Remove slides from the last alcohol after staining 2. Allow to air dry completely (generally only takes a few minutes) 3. Use standard mounting media to coverslip them dry. This works beautifully. Microscopically they look the same as xylene cleared slides. The only caveat is that they must dry completely. -- BETH COX, HTL/SCT(ASCP)QIHC AP Consultant | Pathology Solutions, Inc | (810) 240-2190 | bethcoxx at gmail.com ------------------------------ Message: 6 Date: Tue, 24 May 2016 17:08:51 -0500 From: Mike Toole To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Pap stain without xylene Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB33E1B2A3240 at mail> Content-Type: text/plain; charset="iso-8859-1" Ren? and other Histonetters, After reading the paper: Buesa RJ, Peshkov, MV. Histology without xylene. Ann Diagn Pathol. 2009 Aug;13(4):246-56. Epub 2009 Feb 5. It appears that xylene in the final clearing steps is replaced with isopropanol and mineral oil as follows: ? 5:1 isopropanol to mineral oil 50?C ? 2:1 isopropanol to mineral oil 50?C ? Undiluted mineral oil 50?C ? Drying oven 5 minutes 60?C ? Coverslip Do you feel these clearing steps be applied to the pap stain in order to eliminate xylene? If so, can it be done at room temperature? Thanks, Mike Mike Toole, BS, CT(ASCP)CM From cjohnson at nmda.nmsu.edu Wed May 25 14:58:12 2016 From: cjohnson at nmda.nmsu.edu (Johnson, Carole) Date: Wed, 25 May 2016 19:58:12 +0000 Subject: [Histonet] Factor VIII Message-ID: Hello all, I have been working up the Factor VIII (RTU) on a Bond Max platform with limited success on canine tissue. Does anyone have a favorite that they can recommend? Thanks in advance, Carole Carole L. Johnson, HT(ASCP)cm, QIHC New Mexico Department of Agriculture Veterinary Diagnositc Services 1101 Camino de Salud, NE Albuquerque, NM 87101 505.383.9299 Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From criley at dpspa.com Thu May 26 07:42:25 2016 From: criley at dpspa.com (Charles Riley) Date: Thu, 26 May 2016 08:42:25 -0400 Subject: [Histonet] HPV IHC testing Message-ID: I am looking for a high sensitivity antibody for broad spectrum HPV IHC testing and HPV16 testing. We do not run p16 due to issues with ventana. Anyone have any suggestions on companies and clones to buy? -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From CObregon at mhs.net Thu May 26 08:14:22 2016 From: CObregon at mhs.net (Obregon, Cecilia) Date: Thu, 26 May 2016 13:14:22 +0000 Subject: [Histonet] HPV IHC testing In-Reply-To: References: Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F66990F1E@MHSEXMB03.mhs.net> We have been running the Enzo probes (In-Situ) on the Ventana Ultra since their product was taken out of the market 2-3 years ago. We use both the high and low cocktail. It's working fine for us. Email me separately if you are interested in our protocol. Thank you, Cecilia M. Obregon Memorial Regional Hospital Hollywood, FL ________________________________________ From: Charles Riley via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, May 26, 2016 8:42 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] HPV IHC testing ******************************************************************** THIS EMAIL ORIGINATED FROM OUTSIDE OF MHS. PLEASE EXERCISE CAUTION WITH ATTACHMENTS, LINKS, OR REQUESTED ACTIONS. ******************************************************************** I am looking for a high sensitivity antibody for broad spectrum HPV IHC testing and HPV16 testing. We do not run p16 due to issues with ventana. Anyone have any suggestions on companies and clones to buy? -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. From cjohnson at nmda.nmsu.edu Thu May 26 13:33:26 2016 From: cjohnson at nmda.nmsu.edu (Johnson, Carole) Date: Thu, 26 May 2016 18:33:26 +0000 Subject: [Histonet] Thank you! Message-ID: Thank you to all who have responded with suggestions for Factor VIII, and happy Friday Eve! Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From ewj at pigsqq.org Thu May 26 15:21:36 2016 From: ewj at pigsqq.org (E. Wayne Johnson) Date: Fri, 27 May 2016 04:21:36 +0800 Subject: [Histonet] Pap stain without xylene In-Reply-To: <31530E35E0BAB044B3B56B7FE5CF4EB33E1B2A344D@mail> References: <31530E35E0BAB044B3B56B7FE5CF4EB33E1B2A344D@mail> Message-ID: <1cb4eec7-c720-f17a-eed0-89e9378e8b3b@pigsqq.org> Xylene is becoming more and more of a nuisance material and a problem for us in use and in disposal. We are still able to use it but with increasing concern. We have been able to eliminate xylene from our staining procedures altogether in our small laboratory. We use a hair dryer to dry the slides in the rack after water or alcohol. Since we read our slides right away, we have been using cedarwood oil to clear and mount the slides. It's cheap, and makes lovely slides with no crystals or "floaties" or other artifacts. It's easy to clean up without any xylene or toluene. It's not permanent but we can remount the slides with a permanent mounting medium if we need to keep the slides for some reason. * We also have found several different methods for eliminating xylene from the paraffin infiltration process, and we have not used xylene for dewaxing for more than 2 years now. On 05/26/2016 03:07 AM, Mike Toole wrote: > Thank you Beth, > > It?s good to know that someone else had tried this and had very good results. The method you suggest is very much in line with the recommendations from Ren?. He did recommend using a drying oven at 60?C to help ensure complete removal of any water or alcohol. And, that absolute dryness was a requirement for coverslipping without artifact such as the appearance of sand like grains or cornflakes. > > Just to reiterate, was the field method performed at ambient temperature without the aid of a drying oven? And, just a thought, I suppose if the lack of electricity was an issue in a field setting, that perhaps a solar oven made with plywood and glass could be used to elevate temperature for drying. > > Do you know if altering the method for final clearing would require validation? > > Mike From edmartin26 at gmail.com Thu May 26 15:28:31 2016 From: edmartin26 at gmail.com (Eddie Martin) Date: Thu, 26 May 2016 15:28:31 -0500 Subject: [Histonet] Factor VIII In-Reply-To: References: Message-ID: <9EABE08E-5618-487D-B21F-71AE858774D6@gmail.com> Hi Carol, You probably are getting inconsistent results with Novocastra Factor 8 antibody because the Novocastra RTU is for human diagnostic testing. If you need it for canine testing and would still need to use the bond max to perform the test, then you would need a canine anti-mouse or Canine anti-rabbit preferably monoclonal antibody. Kind Regards, Eddie Martin HT(ASCP),QIHC 954-826-9403 Sent from my iPhone > On May 25, 2016, at 2:58 PM, Johnson, Carole wrote: > > Hello all, > I have been working up the Factor VIII (RTU) on a Bond Max platform with limited success on canine tissue. Does anyone have a favorite that they can recommend? > > Thanks in advance, > Carole > > Carole L. Johnson, HT(ASCP)cm, QIHC > > New Mexico Department of Agriculture > Veterinary Diagnositc Services > 1101 Camino de Salud, NE > Albuquerque, NM 87101 > 505.383.9299 > > > > > Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. > From ihcworkshop at gmail.com Thu May 26 23:10:11 2016 From: ihcworkshop at gmail.com (Ihc Workshop) Date: Thu, 26 May 2016 21:10:11 -0700 Subject: [Histonet] IHC Wet Workshop announcement Message-ID: <7FD71287-86BF-4D1E-A29C-62ADF362BA89@gmail.com> A venue to learn IHC staining hands-on; This is a small group IHC Lab course for learning IHC staining of human, mouse and animal tissues and IHC troubleshooting June 23 and 24, 2016 San Francisco Bay Area.....approved for 12 CEU. Very few spots left. For more details contact Maria at ihcworkshop at gmail.com From a_schade at conradweiser.org Fri May 27 06:29:53 2016 From: a_schade at conradweiser.org (Schade, Adelle) Date: Fri, 27 May 2016 11:29:53 +0000 Subject: [Histonet] thank you HistoNet! In-Reply-To: References: Message-ID: Hello Histonet, I am a high school science teacher and part-time PhD student in Cell and Molecular Biology. I started a histology/ biomedical research laboratory in my high school two years ago and it has been quite an experience. This year, two of my students who incorporated histological testing into their science fair project protocols won awards at the INTEL International Science and Engineering Competition in Phoenix, AZ. They are considered in the top 20% of high school research projects in the world! Many other Conrad Weiser High School students won awards at our regional fair incorporating histology into their laboratory techniques as well. This listserv was vital to their success. I emailed the listserv last year asking for donations to support our summer program and the response was overwhelming. Thank you for supporting histology in high school. The students are learning and loving it! I receive advice from this listserv as well in reference to our processes. Your advice and words of wisdom are a key component to our success as a program! We are operating our summer program again this year. If anyone knows of a laboratory that is upgrading equipment, closing, etc. and has used equipment that is still functioning, we would appreciate if you would forward our school information. We can use any lab equipment, reagents, solutions and consumables used in histology, cell culturing or biotechnological processes. Again, HistoNet, thank you for your continued support of our Conrad Weiser High School family! Have a nice day, Adelle Ms. Adelle L. Schade, B.S., M.Ed., M.S. Biomedical Science Conrad Weiser High School 44 Big Spring Rd. Robesonia, PA 19551 610-693-8599 x6783 a_schade at conradweiser.org From relia1 at earthlink.net Fri May 27 09:48:47 2016 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 27 May 2016 10:48:47 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin 5/27/2016 Happy Memorial Day Here's a Twist on the Traditional for your Memorial Day BBQ Message-ID: <006101d1b826$dfea0920$9fbe1b60$@earthlink.net> Hi Histonetters! Summer is almost here already! It seems like this year is flying by. ? This weekend is Memorial Day Weekend. I want to wish you a safe and Happy Memorial Day Weekend! ? Since this weekend is probably the first of many cookouts and summer get-togethers I am curious When you have to bring something to a cookout or party what is your go to item? ? If you want to try a trendy twist on a traditional summer treat I recommend a Watermelon and Feta Salad. I would be happy to send you the link to my favorite version. ? So tell me if I invited you to a cookout this weekend what would you bring? ? Before I tell you about my current openings I need to apologize. I have been having some problems with my email and if you have replied to me and not heard back that is why. I ALWAYS respond to emails within 24 hours. So please know that if you haven?t heard back from me it was a technical difficulty that has been resolved and it won?t happen again. ? Here are my current job openings: ? RELIA?s Current Histology Opportunities: Amarillo, TX Histology Supervisor Nashville, TN Histology Supervisor Roswell, NM Assistant Supervisor Fayetteville, AR Lead Dermpath Histotech Norfolk, VA Histology tech Nashville, TN Histotechnician Milwaukee, WI Histotech Flagstaff, AZ Histo tech II Austin, TX Histotechnician Birmingham, AL Histotech Glenwood Springs, CO Histotechnician ? These opportunities are with some of the best facilities in the U.S. They offer growth, training, opportunity for advancement and great people to work with. All of these positions are full time permanent positions and my clients offer a great compensation, benefits and relocation assistance/sign on bonuses. ? ? If you or anyone you know might be interested in any of these positions or want help with a job search in another area please contact me. I can be reached at 866-607-3542 or relia1 at earthlink.net Please feel free to contact me ASAP or shoot me an e-mail to set up a time to talk after the holiday weekend. ? If you want this recipe let me know. Also I can?t wait to hear what you would bring! Thanks-Pam ? Right Place, Right Time, Right Move with RELIA! ? Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From koellingr at comcast.net Fri May 27 10:49:05 2016 From: koellingr at comcast.net (koellingr at comcast.net) Date: Fri, 27 May 2016 15:49:05 +0000 (UTC) Subject: [Histonet] thank you HistoNet! Histology STEM long off-topic In-Reply-To: References: Message-ID: <1618908490.7137020.1464364144997.JavaMail.zimbra@comcast.net> Histonet- ? I cringe at the thought of being?dinged for taking time with something?somewhat tangential to histology but here I go.? If histology as part of STEM (science, technology, engineering, math) and kids' futures is a bit overboard for you, please use your delete button now. ? First Adelle Schade should be congratulated profusely for her work with her students?and starting?their high school histology/biomedical research lab.? I myself helped and donated to a local (Seattle) high school biotech/biomed lab where we (they) did some fairly sophisticated IHC and molecular techniques. I am also very familiar with the Intel International Science and Engineering Competition in Phoenix.? We in Washington state have recently?sent 20 kids there and they have returned.? Involved with STEM educational outreach for our state K-12 for 15 years, I am currently re-elected to our Board of the state fair, WSSEF (Washington State Science and Engineering Fair).? Intel affiliated, we sent those 20 or so kids to Phoenix, along with Adelle's kids, to compete against close to 2,000 high schoolers from every state and 70 countries around the world. ? Our son is safely working on his Masters degree in Aeronautical Engineering so he will be in good shape.? But I am very worried for many, many kids in the US for an almost systemic rejection of science in lieu of nearly 100% sports, art and music.? I love sports having played baseball in high school and college.? I love artistic endeavors be it at a symphony, art museum or stage play.? I love music even though it might be quite different from the music many enjoy today.? But I also love to eat well and live well and enjoyed saving up for a comfortable retirement.? Can you do those things in non-STEM pursuits? Of course you can!? Yet the fact is that very, very, very few K-12 students will ever make a great future life as a professional athlete, artist or musician.? Yet in the world of the future, the majority of great jobs are in STEM.? That is not an opinion.? That is simply reality. ? So I encourage anyone to get involved with teachers in high school such as Adelle Schade. Histology is obviously the topic of discussion here but it can be with anything?STEM.? Depending on which study you look at, we (the US) are 15th-25th amongst?nations in the world in K-12 science and math education.? Far too many of the kids in the US are being left behind for the jobs of the future. ? Having "retired" to Spokane Washington, I now find myself?as a part-time lecturer at the University of Washington Medical School-Spokane campus, lecturing in microanatomy to first year medical students in their pre-clinical required curriculum.? Yet I still will be helping K-12 students around here in their local science fairs and also with the Washington State Science and Engineering Fair in preparation for the May 2017 INTEL international fair to be held in Los Angeles, CA. ?A few more histology/molecular/IHC/biotech/biomed? projects would be AWESOME. ? Thanks to people like Adelle and I encourage everyone?with helping K-12 kids and their teachers with educational outreach?for science fairs?in histology or anything STEM . ? Ray Koelling UW Med School-Spokane campusl lecturer, microanatomy ----- Original Message ----- From: "Adelle via Histonet Schade" To: "histonet at lists.utsouthwestern.edu" Sent: Friday, May 27, 2016 4:29:53 AM Subject: [Histonet] thank you HistoNet! Hello Histonet, I am a high school science teacher and part-time PhD student in Cell and Molecular Biology. I started a histology/ biomedical research laboratory in my high school two years ago and it has been quite an experience. ?This year, two of my students who incorporated histological testing into their science fair project protocols won awards at the INTEL International Science and Engineering Competition in Phoenix, AZ. ?They are considered in the top 20% of high school research projects in the world! ?Many other Conrad Weiser High School students won awards at our regional fair incorporating histology into their laboratory techniques as well. This listserv was vital to their success. ?I emailed the listserv last year asking for donations to support our summer program and the response was overwhelming. ?Thank you for supporting histology in high school. ?The students are learning and loving it! ?I receive advice from this listserv as well in reference to our processes. ?Your advice and words of wisdom are a key component to our success as a program! We are operating our summer program again this year. ?If anyone knows of a laboratory that is upgrading equipment, closing, etc. and has used equipment that is still functioning, we would appreciate if you would forward our school information. ?We can use any lab equipment, reagents, solutions and consumables used in histology, cell culturing or biotechnological processes. Again, HistoNet, thank you for your continued support of our Conrad Weiser High School family! Have a nice day, Adelle Ms. Adelle L. Schade, B.S., M.Ed., M.S. Biomedical Science Conrad Weiser High School 44 Big Spring Rd. Robesonia, PA ?19551 610-693-8599 x6783 a_schade at conradweiser.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz at premierlab.com Fri May 27 11:30:16 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Fri, 27 May 2016 10:30:16 -0600 Subject: [Histonet] thank you HistoNet! Histology STEM long off-topic In-Reply-To: <1618908490.7137020.1464364144997.JavaMail.zimbra@comcast.net> References: <1618908490.7137020.1464364144997.JavaMail.zimbra@comcast.net> Message-ID: <14E2C6176416974295479C64A11CB9AE02BEC2AD6060@SBS2K8.premierlab.local> The NSH as a part of its awards and scholarships program has the Newcomer Helping Hand Scholarship. If you are considering doing something like what Adelle or Ray did and are a NSH member for two years you can apply for this scholarship. The scholarship is for $2000.00 so it is quite substantial. The deadline for awards nomination is June 1st, so there is still time to nominate yourself. Here is the link http://nsh.org/content/newcomer-helping-hand-scholarship Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Ray via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, May 27, 2016 9:49 AM To: Adelle Schade Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] thank you HistoNet! Histology STEM long off-topic Histonet- ? I cringe at the thought of being?dinged for taking time with something?somewhat tangential to histology but here I go.? If histology as part of STEM (science, technology, engineering, math) and kids' futures is a bit overboard for you, please use your delete button now. ? First Adelle Schade should be congratulated profusely for her work with her students?and starting?their high school histology/biomedical research lab.? I myself helped and donated to a local (Seattle) high school biotech/biomed lab where we (they) did some fairly sophisticated IHC and molecular techniques. I am also very familiar with the Intel International Science and Engineering Competition in Phoenix.? We in Washington state have recently?sent 20 kids there and they have returned.? Involved with STEM educational outreach for our state K-12 for 15 years, I am currently re-elected to our Board of the state fair, WSSEF (Washington State Science and Engineering Fair).? Intel affiliated, we sent those 20 or so kids to Phoenix, along with Adelle's kids, to compete against close to 2,000 high schoolers from every state and 70 countries around the world. ? Our son is safely working on his Masters degree in Aeronautical Engineering so he will be in good shape.? But I am very worried for many, many kids in the US for an almost systemic rejection of science in lieu of nearly 100% sports, art and music.? I love sports having played baseball in high school and college.? I love artistic endeavors be it at a symphony, art museum or stage play.? I love music even though it might be quite different from the music many enjoy today.? But I also love to eat well and live well and enjoyed saving up for a comfortable retirement.? Can you do those things in non-STEM pursuits? Of course you can!? Yet the fact is that very, very, very few K-12 students will ever make a great future life as a professional athlete, artist or musician.? Yet in the world of the future, the majority of great jobs are in STEM.? That is not an opinion.? That is simply reality. ? So I encourage anyone to get involved with teachers in high school such as Adelle Schade. Histology is obviously the topic of discussion here but it can be with anything?STEM.? Depending on which study you look at, we (the US) are 15th-25th amongst?nations in the world in K-12 science and math education.? Far too many of the kids in the US are being left behind for the jobs of the future. ? Having "retired" to Spokane Washington, I now find myself?as a part-time lecturer at the University of Washington Medical School-Spokane campus, lecturing in microanatomy to first year medical students in their pre-clinical required curriculum.? Yet I still will be helping K-12 students around here in their local science fairs and also with the Washington State Science and Engineering Fair in preparation for the May 2017 INTEL international fair to be held in Los Angeles, CA. ?A few more histology/molecular/IHC/biotech/biomed? projects would be AWESOME. ? Thanks to people like Adelle and I encourage everyone?with helping K-12 kids and their teachers with educational outreach?for science fairs?in histology or anything STEM . ? Ray Koelling UW Med School-Spokane campusl lecturer, microanatomy ----- Original Message ----- From: "Adelle via Histonet Schade" To: "histonet at lists.utsouthwestern.edu" Sent: Friday, May 27, 2016 4:29:53 AM Subject: [Histonet] thank you HistoNet! Hello Histonet, I am a high school science teacher and part-time PhD student in Cell and Molecular Biology. I started a histology/ biomedical research laboratory in my high school two years ago and it has been quite an experience. ?This year, two of my students who incorporated histological testing into their science fair project protocols won awards at the INTEL International Science and Engineering Competition in Phoenix, AZ. ?They are considered in the top 20% of high school research projects in the world! ?Many other Conrad Weiser High School students won awards at our regional fair incorporating histology into their laboratory techniques as well. This listserv was vital to their success. ?I emailed the listserv last year asking for donations to support our summer program and the response was overwhelming. ?Thank you for supporting histology in high school. ?The students are learning and loving it! ?I receive advice from this listserv as well in reference to our processes. ?Your advice and words of wisdom are a key component to our success as a program! We are operating our summer program again this year. ?If anyone knows of a laboratory that is upgrading equipment, closing, etc. and has used equipment that is still functioning, we would appreciate if you would forward our school information. ?We can use any lab equipment, reagents, solutions and consumables used in histology, cell culturing or biotechnological processes. Again, HistoNet, thank you for your continued support of our Conrad Weiser High School family! Have a nice day, Adelle Ms. Adelle L. Schade, B.S., M.Ed., M.S. Biomedical Science Conrad Weiser High School 44 Big Spring Rd. Robesonia, PA ?19551 610-693-8599 x6783 a_schade at conradweiser.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garethdavisyuma at gmail.com Fri May 27 12:47:44 2016 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Fri, 27 May 2016 10:47:44 -0700 Subject: [Histonet] Ventana/Roche H. pylori antibody Message-ID: Hi, I have read on the Histonet, in the past, that other labs are having trouble with the H.pylori antibody from Ventana/Roche. I get "debris" that looks like the H. pylori bug, except it appears to "float" above the section and not in the normal areas that H. pylori stains. Is anyone else still having the problem? Or if you had the problem, was it resolved? Roche did a decon on our machine on two different occasions, which did not help. Now Roche seems to think that our machine is old and causing the problem. Anyone's help would be appreciated. Thanks -- Gareth B. Davis, HT (ASCPcm), QIHCcm Yuma Gastroenterology From carla.thompson at vwr.com Fri May 27 13:27:59 2016 From: carla.thompson at vwr.com (carla.thompson at vwr.com) Date: Fri, 27 May 2016 14:27:59 -0400 Subject: [Histonet] FFPE human tissue blocks Message-ID: Hello Histonet, My lab is looking for FFPE human tissue blocks, in good condition, that are no longer needed. Both normal and diseased tissue. Please let me know of any available sources for these unwanted or no longer needed blocks. Thanks, Carla ******************************************** Carla Thompson Senior Specimen Buyer Microscope Slides Lab a division of VWR International, LLC. 5100 West Henrietta Road Post Office Box 92912 Rochester, New York 14692-9012 From raestask at grics.net Fri May 27 14:49:44 2016 From: raestask at grics.net (Rae Staskiewicz) Date: Fri, 27 May 2016 14:49:44 -0500 Subject: [Histonet] HHV8 Antibody Message-ID: <013b01d1b850$f14db070$d3e91150$@grics.net> Has anyone had experience with this antibody? One of our Hemepaths wants to bring this on line. Where do you get your antibody and what is your protocol? Any info would be much appreciated. Rae Staskiewicz UnityPoint Health-Methodist/Proctor From garethdavisyuma at gmail.com Fri May 27 15:01:13 2016 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Fri, 27 May 2016 13:01:13 -0700 Subject: [Histonet] Ventana/Roche H. pylori Message-ID: Thank you to all who have been responding. Very helpful information. I have been seeing this problem for almost a year. We have contacted YRMC and know their option. Unfortunately, we are under contract. But, the previous lab I worked in had the same issue, but with DAKO. But, the debris showed up in the negative slides also. They finally started only using fresh DAB, it made a difference. Wonder if they should filter everything too. -- Gareth B. Davis, HT (ASCPcm), QIHC Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From teresajharris at msn.com Sat May 28 18:28:38 2016 From: teresajharris at msn.com (Teresa harris) Date: Sat, 28 May 2016 18:28:38 -0500 Subject: [Histonet] FFPE human tissue blocks Message-ID: I have some Carla. Please contact me if you are still in need. Teresa Harris, HTL (ASCP) QIHC (618) 203-1993 Cell Phone Sent from Mail for Windows 10 From: C via Histonet Sent: Friday, May 27, 2016 1:51 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] FFPE human tissue blocks Hello Histonet, My lab is looking for FFPE human tissue blocks, in good condition, that are no longer needed. Both normal and diseased tissue. Please let me know of any available sources for these unwanted or no longer needed blocks. Thanks, Carla ******************************************** Carla Thompson Senior Specimen Buyer Microscope Slides Lab a division of VWR International, LLC. 5100 West Henrietta Road Post Office Box 92912 Rochester, New York 14692-9012 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 at cdc.gov Sun May 29 08:57:16 2016 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Sun, 29 May 2016 13:57:16 +0000 Subject: [Histonet] Special stains performed on H&E stainers Message-ID: <3B2CD438E1628A41BD687E98B963B781690E85B8@EMBX-CLFT4.cdc.gov> Morning all! Does anyone have experience doing specials, esp. Gram stains, on the Sakura Prisma and/or the Leica ST5020 multistainer? Thanks much and Happy Memorial Day tomorrow! Jeanine H. Sanders Infectious Diseases Pathology Branch Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS-G32 Atlanta, GA 30329 From tbraud at holyredeemer.com Mon May 30 11:19:37 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 30 May 2016 16:19:37 +0000 Subject: [Histonet] Dirty HP Antibody Message-ID: <48E053DDF6CE074DB6A7414BA05403F80776E5@HRHEX02-HOS.holyredeemer.local> We had the same issue a few years ago with the Ventana HP antibody. Then we tried the antibody from Zymed and it was so much better. Now we use BioCare's antibody, and it is very clean. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 From criley at dpspa.com Tue May 31 08:00:27 2016 From: criley at dpspa.com (Charles Riley) Date: Tue, 31 May 2016 09:00:27 -0400 Subject: [Histonet] Histology tips Message-ID: I am trying to do a histology tip of the week for my new histo team as a way to help them learn some new ways to do troubleshooting. I've run out of ideas at the moment. If anyone has any fun, interesting, or extremely useful tip for troubleshooting any issues please send them to me. I would really appreciate it. I also just want to add in a thank you for all the help I have received from all our users. Everyone has been so helpful and I have been able to fix a large amount of errors my predecessors has left piled up for me to fix. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From nelsonrnch at verizon.net Tue May 31 08:28:04 2016 From: nelsonrnch at verizon.net (Patti Nelson - PNP Lab Consultant) Date: Tue, 31 May 2016 09:28:04 -0400 Subject: [Histonet] Sakura Prisma 4740 combo film Message-ID: <15506ffeda9-2ec1-7748@webprd-a15.mail.aol.com> Hi everyone, I am considering placing a Sakura Prisma 4740 combo film in one of my labs. My question is , does the cover slip unit need an external exhaust. Please consider that the space the unit will be placed is small. Thank you in advance. Sincerely, PATTI NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR DGC/ZADEH LABS PO BOX 412 CABAZON, CA. 92230 909-841-9761 nelsonrnch at verizon.net CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender ofthe delivery error by e-mail or you may call 909-841-9761. From jqb7 at cdc.gov Tue May 31 08:55:04 2016 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue, 31 May 2016 13:55:04 +0000 Subject: [Histonet] those slippery lab floors Message-ID: <3B2CD438E1628A41BD687E98B963B781690E8DDE@EMBX-CLFT4.cdc.gov> All, Do any of you use floor mats to help with paraffin issues around microtomy stations? Thanks! Jeanine H. Sanders Infectious Diseases Pathology Branch Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS-G32 Atlanta, GA 30329 From liz at premierlab.com Tue May 31 09:50:15 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Tue, 31 May 2016 08:50:15 -0600 Subject: [Histonet] Sakura Prisma 4740 combo film In-Reply-To: <15506ffeda9-2ec1-7748@webprd-a15.mail.aol.com> References: <15506ffeda9-2ec1-7748@webprd-a15.mail.aol.com> Message-ID: <14E2C6176416974295479C64A11CB9AE02BEC2AD6067@SBS2K8.premierlab.local> Patti We have the Sakura Prisma with the Glas coverslipper and we have ours vented out. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Patti Nelson - PNP Lab Consultant via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 31, 2016 7:28 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Sakura Prisma 4740 combo film Hi everyone, I am considering placing a Sakura Prisma 4740 combo film in one of my labs. My question is , does the cover slip unit need an external exhaust. Please consider that the space the unit will be placed is small. Thank you in advance. Sincerely, PATTI NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR DGC/ZADEH LABS PO BOX 412 CABAZON, CA. 92230 909-841-9761 nelsonrnch at verizon.net CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender ofthe delivery error by e-mail or you may call 909-841-9761. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LNormington at uwhealth.org Tue May 31 09:51:35 2016 From: LNormington at uwhealth.org (Normington Lacy) Date: Tue, 31 May 2016 14:51:35 +0000 Subject: [Histonet] those slippery lab floors In-Reply-To: <3B2CD438E1628A41BD687E98B963B781690E8DDE@EMBX-CLFT4.cdc.gov> References: <3B2CD438E1628A41BD687E98B963B781690E8DDE@EMBX-CLFT4.cdc.gov> Message-ID: <7F7174244DDD1A49BFBEE8A845BC2F2B1CF5A5@UWHC-MBX05.uwhis.hosp.wisc.edu> Our laboratory has custodial staff put down a multi surface protection film. The film is sticky on one side and comes on a roll. That way it can be pulled back up and changed out. Go to www.surfaceshields.com. Search "Multipurpose surface protection film:" This stuff works wonders and the custodial staff love it because they don't have to scrape the floors. We have ours changed out at least monthly, but call sooner if it starts to look bad before then. Hope this helps. Lacy Normington Lacy Normington, HTL(ASCP)CM Manager, Surgical Pathology Lab Services UWHospital and Clinics 600 Highland Avenue Madison, WI 53792-2472 Phone: 608-890-9373 -----Original Message----- From: Sanders, Jeanine (CDC/OID/NCEZID) via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 31, 2016 8:55 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] those slippery lab floors All, Do any of you use floor mats to help with paraffin issues around microtomy stations? Thanks! Jeanine H. Sanders Infectious Diseases Pathology Branch Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS-G32 Atlanta, GA 30329 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sandra.cheasty at wisc.edu Tue May 31 09:54:28 2016 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Tue, 31 May 2016 14:54:28 +0000 Subject: [Histonet] Program for organizing and re-naming digital gross photos Message-ID: Hello all, Picasa, the program we currently use for re-naming and organizing our gross digital photos, will no longer be supported by Google. If anyone can suggest another program for this purpose, please let me know. We don't need any fancy editing of the photos themselves other than cropping and adjusting brightness; mostly just for re-naming and organizing by species. Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From Heather.Seeley at tenethealth.com Tue May 31 10:13:38 2016 From: Heather.Seeley at tenethealth.com (Seeley, Heather) Date: Tue, 31 May 2016 15:13:38 +0000 Subject: [Histonet] Dirty HP Antibody In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F80776E5@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F80776E5@HRHEX02-HOS.holyredeemer.local> Message-ID: <9DFE334E776E734D9D231EF60CCE93C57E2826F0@TENHDCTHMB10-31.tenethealth.net> We also had the same problem with Ventana. We are currently using Cell Marque and it is working great! HEATHER SEELEY, HT(ASCP) Histotech 803-985-4676 OFFICE 803-327-7598 FAX ________________________________________ From: Terri Braud [tbraud at holyredeemer.com] Sent: Monday, May 30, 2016 12:19 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Dirty HP Antibody We had the same issue a few years ago with the Ventana HP antibody. Then we tried the antibody from Zymed and it was so much better. Now we use BioCare's antibody, and it is very clean. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 From rjbuesa at yahoo.com Tue May 31 12:36:35 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 31 May 2016 17:36:35 +0000 (UTC) Subject: [Histonet] Histology tips In-Reply-To: References: Message-ID: <1388531700.1855321.1464716195815.JavaMail.yahoo@mail.yahoo.com> Would you share what you receive for the amusement/benefit of us all?Ren? On Tuesday, May 31, 2016 9:15 AM, Charles Riley via Histonet wrote: I am trying to do a histology tip of the week for my new histo team as a way to help them learn some new ways to do troubleshooting. I've run out of ideas at the moment. If anyone has any fun, interesting, or extremely useful tip for troubleshooting any issues please send them to me. I would really appreciate it. I also just want to add in a thank you for all the help I have received from all our users. Everyone has been so helpful and I have been able to fix a large amount of errors my predecessors has left piled up for me to fix. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carla.thompson at vwr.com Tue May 31 14:38:08 2016 From: carla.thompson at vwr.com (carla.thompson at vwr.com) Date: Tue, 31 May 2016 15:38:08 -0400 Subject: [Histonet] Subject: Re: FFPE human tissue blocks Message-ID: Thanks so much, Theresa. I'll give you a call today. I appreciate your help. Carla ******************************************** Carla Thompson Senior Specimen Buyer Microscope Slides Lab a division of VWR International, LLC. 5100 West Henrietta Road Post Office Box 92912 Rochester, New York 14692-9012 From SteveM at mcclainlab.com Tue May 31 16:48:42 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Tue, 31 May 2016 21:48:42 +0000 Subject: [Histonet] Histonet Digest, Vol 150, Issue 31 5. Re: those slippery lab floors (Normington Lacy) Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DFC043B0BE@ML1.McClainLabs.local> I did see many slip and fall injuries in histolabs over the years. When we built this lab we accounted for that and deliberately left the bare concrete rough, and professionally painted polyurethane of the type used in firehouse floors. Has held up reasonably well. No slips or falls in 12 years. Some stains; Would not use in grossing room again however. Drop a specimen fragment on that floor and you may be searching for an hour. - Steve A. McClain, MD 631 361 4000