[Histonet] Improving fixation of insect parts for TEM

[Jorge A. Santiago-Blay]

Hello Histonet-Listers:

This is a follow up on earlier messages re. fixation of insect parts for
TEM.

First, thanks for the feedback to try minimizing the effect of setae in
creating an air bubble.

Second, we are having difficulties making the resin for blocks used in TEM
adhere well to the insect exoskeleton.It seems that there is something on
the exoskeleton preventing this. Any constructive suggestions (e.g. washing
the insect with a strong solvent?) to improve adherence will be
appreciated. My email is blayjorge at gmail.com

Gratefully,

Jorge

Jorge A. Santiago-Blay, PhD
blaypublishers.com

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