[Histonet] cryosectioning undecalcified samples

[Caroline Miller]

In this space there is the cryojane (expensive,
http://www.leicabiosystems.com/research/neuroscience/details/product/tape-transfer-system/)
or the Japanese Kawamoto method:
http://section-lab.jp/English/Introduction.htm

I have also found that if you take a regular piece of scotch tape and put
it on the block prior to sectioning that it also works :) (trim to your
area of interest, move the blade along, place on the tape and press down
(use a tool to do this - don't use your hand to reduce the warming of the
tissue, which would give too big a slice), then flip the scotch tape to the
outside of the blade and take a section. You can then stain the tissue on
the tape, dehydrate and clear, then stick the whole thing under a
coverslip. Crude but effective for standard stains. I could not get
immunostaining to work with it though......

It is hard to then get the tissue off the tape - apparently it works with
hexane for the Kawamoto method, but I could never get that to work

Ultimately if you were going to buy something I would say the Kawamoto is a
great place to start, they (he) has a bunch of good tools in cute boxes
that you can work with to make the process easier

Good Luck!

mills



On Wed, Mar 2, 2016 at 11:36 AM, M.O. via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hello,
>
> I am looking into options for cryosectioning undecalcified mouse knees.
> One of my co-workers said he used a film in Japan that stuck to the
> cryoblock and then would do IHC directly on this tape.
>
> Has anyone cut undecalcified cryosections?  If so, what options are there
> and do you recommend one over the other?
>
> Thank you,
> Merissa
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-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297