From histo at pathlab.us Tue Mar 1 11:15:38 2016 From: histo at pathlab.us (Histology) Date: Tue, 1 Mar 2016 17:15:38 +0000 Subject: [Histonet] Freeze Spary Ban continued Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD823458AA@vdc01.domain.local> FYI: Mercedes Medical DOES contain the banned chemical. StatLab's 1,1,1,2 Tetrafluoroethane is banned. This is also called HFC 134a Still checking other brands. -Mehndi From srishan at mail.holyname.org Tue Mar 1 11:51:27 2016 From: srishan at mail.holyname.org (srishan at mail.holyname.org) Date: Tue, 1 Mar 2016 12:51:27 -0500 Subject: [Histonet] PARTS FOR SAKURA COVERSLIPPER Message-ID: Hi All, We are looking for parts for the Tape Coverslipper by Sakura -Tissure Tek SCA. The Blade holder which cuts the tape needs replacement. Does any one know a company which sells parts for this. Sakura does not carry this part anymore. I thank you in advance! Nirmala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From Gail.Macke at cchmc.org Tue Mar 1 12:13:14 2016 From: Gail.Macke at cchmc.org (Macke, Gail) Date: Tue, 1 Mar 2016 18:13:14 +0000 Subject: [Histonet] Freeze Spary Ban continued In-Reply-To: <09CFA3F99D5B2B42B88CDFB2FC4CFD823458AA@vdc01.domain.local> References: <09CFA3F99D5B2B42B88CDFB2FC4CFD823458AA@vdc01.domain.local> Message-ID: <648AE493-95B3-44C0-8C64-2FEC2BD8CB61@cchmc.org> FYI: Just looked at Leica/Surgipath?s Frostbite and it has the 1,1,1,2 Tetrafluoroethane in it ,too. Gail Macke, Cincinnati Childrens Hospital Medical Center On Mar 1, 2016, at 12:15 PM, Histology via Histonet > wrote: FYI: Mercedes Medical DOES contain the banned chemical. StatLab's 1,1,1,2 Tetrafluoroethane is banned. This is also called HFC 134a Still checking other brands. -Mehndi _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQICAg&c=P0c35rBvlN7D8BNx7kSJTg&r=EHoZsUPFU92qg6yiYwM5gy-CtQoI_1E8mAl9MzLu1wM&m=8Bqfye7yLupm62E4ro4YiQmrQ7iVpvaFhaBHIKn8gKw&s=kKwXSH1ofdUzWU2zcVaXZgqNOlLGdRM112PF0SsbW9k&e= From jpiche at wtbyhosp.org Tue Mar 1 12:15:53 2016 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Tue, 1 Mar 2016 18:15:53 +0000 Subject: [Histonet] FW: Freeze Spary Ban continued In-Reply-To: <648AE493-95B3-44C0-8C64-2FEC2BD8CB61@cchmc.org> References: <09CFA3F99D5B2B42B88CDFB2FC4CFD823458AA@vdc01.domain.local> <648AE493-95B3-44C0-8C64-2FEC2BD8CB61@cchmc.org> Message-ID: <631955447A364B45B9458D2905635110010774AE2D@WIN08-MBX-01.wtbyhosp.org> Just checked ours: Richard Allan Scientific CYTOCOOL II has it as well!! Jessica Piche, HT(ASCP) Waterbury Hospital -----Original Message----- From: Macke, Gail via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, March 01, 2016 1:13 PM To: Histology Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Freeze Spary Ban continued FYI: Just looked at Leica/Surgipath's Frostbite and it has the 1,1,1,2 Tetrafluoroethane in it ,too. Gail Macke, Cincinnati Childrens Hospital Medical Center On Mar 1, 2016, at 12:15 PM, Histology via Histonet > wrote: FYI: Mercedes Medical DOES contain the banned chemical. StatLab's 1,1,1,2 Tetrafluoroethane is banned. This is also called HFC 134a Still checking other brands. -Mehndi _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQICAg&c=P0c35rBvlN7D8BNx7kSJTg&r=EHoZsUPFU92qg6yiYwM5gy-CtQoI_1E8mAl9MzLu1wM&m=8Bqfye7yLupm62E4ro4YiQmrQ7iVpvaFhaBHIKn8gKw&s=kKwXSH1ofdUzWU2zcVaXZgqNOlLGdRM112PF0SsbW9k&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From tbraud at holyredeemer.com Tue Mar 1 12:32:00 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 1 Mar 2016 18:32:00 +0000 Subject: [Histonet] PA help sought Message-ID: <48E053DDF6CE074DB6A7414BA05403F806323C@HRHEX02-HOS.holyredeemer.local> Our lab is seeking an Pathology Assistant for an immediate temporary position for all gross responsibilities. The position will be approximately 10-12 weeks. We are a medium sized hospital in the NE suburbs of Philadelphia. We have a friendly technical and medical staff of 2.5 pathologists and 3 technicians. Our block load averages about 180/day with a range of 60 to 320, and an even mix of large and small specimens. Breast pathology a plus. M-F day shift only. Agencies are welcome to respond. Thanks, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 **************************************** From Shannon.Logan at bellin.org Tue Mar 1 14:12:43 2016 From: Shannon.Logan at bellin.org (Logan, Shannon) Date: Tue, 1 Mar 2016 20:12:43 +0000 Subject: [Histonet] FW: FW: Freeze Spray Ban continued Message-ID: Subject: RE: [Histonet] FW: Freeze Spray Ban continued http://www.fridgehub.com/News/epa-fact-sheet-and-timelines-for-proposed-hfc-bans After reading this article, seems to affect only cars, refrigerators, and certain air conditioning units.... Shannon H. Logan B.S. HTL (ASCP) Pathology Dept. Bellin Health 744 S Webster Ave Green Bay, WI 54305 920-433-3653 -----Original Message----- From: Piche, Jessica via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, March 01, 2016 12:16 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] FW: Freeze Spary Ban continued Just checked ours: Richard Allan Scientific CYTOCOOL II has it as well!! Jessica Piche, HT(ASCP) Waterbury Hospital -----Original Message----- From: Macke, Gail via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, March 01, 2016 1:13 PM To: Histology Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Freeze Spary Ban continued FYI: Just looked at Leica/Surgipath's Frostbite and it has the 1,1,1,2 Tetrafluoroethane in it ,too. Gail Macke, Cincinnati Childrens Hospital Medical Center On Mar 1, 2016, at 12:15 PM, Histology via Histonet > wrote: FYI: Mercedes Medical DOES contain the banned chemical. StatLab's 1,1,1,2 Tetrafluoroethane is banned. This is also called HFC 134a Still checking other brands. -Mehndi _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQICAg&c=P0c35rBvlN7D8BNx7kSJTg&r=EHoZsUPFU92qg6yiYwM5gy-CtQoI_1E8mAl9MzLu1wM&m=8Bqfye7yLupm62E4ro4YiQmrQ7iVpvaFhaBHIKn8gKw&s=kKwXSH1ofdUzWU2zcVaXZgqNOlLGdRM112PF0SsbW9k&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nlinke at sbch.org Tue Mar 1 14:26:11 2016 From: nlinke at sbch.org (Noelle Linke) Date: Tue, 1 Mar 2016 20:26:11 +0000 Subject: [Histonet] FW: FW: Freeze Spray Ban continued Message-ID: It has been delayed until 2020. We have 4 years to figure it out.... http://www.tradeonlytoday.com/2015/07/epa-delays-ban-hfc-blowing-agents/ Thank you, No?lle No?lle Linke, MS, HTL(ASCP) QIHC Manager, Anatomic Pathology Pacific Diagnostic Laboratories Cottage Health Systems nlinke at sbch.org Phone: (805) 324-9814 Fax: (805) 696-6433 -----Original Message----- From: Piche, Jessica via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, March 01, 2016 10:16 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] FW: Freeze Spary Ban continued Just checked ours: Richard Allan Scientific CYTOCOOL II has it as well!! Jessica Piche, HT(ASCP) Waterbury Hospital -----Original Message----- From: Macke, Gail via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, March 01, 2016 1:13 PM To: Histology Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Freeze Spary Ban continued FYI: Just looked at Leica/Surgipath's Frostbite and it has the 1,1,1,2 Tetrafluoroethane in it ,too. Gail Macke, Cincinnati Childrens Hospital Medical Center On Mar 1, 2016, at 12:15 PM, Histology via Histonet > wrote: FYI: Mercedes Medical DOES contain the banned chemical. StatLab's 1,1,1,2 Tetrafluoroethane is banned. This is also called HFC 134a Still checking other brands. -Mehndi _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQICAg&c=P0c35rBvlN7D8BNx7kSJTg&r=EHoZsUPFU92qg6yiYwM5gy-CtQoI_1E8mAl9MzLu1wM&m=8Bqfye7yLupm62E4ro4YiQmrQ7iVpvaFhaBHIKn8gKw&s=kKwXSH1ofdUzWU2zcVaXZgqNOlLGdRM112PF0SsbW9k&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ CH Disclaimer: This electronic mail message is intended exclusively for the individual or entity to which it is addressed. This message, together with any attachment, may contain confidential and privileged information. Any views, opinions or conclusions expressed in this message are those of the individual sender and do not necessarily reflect the views of Cottage Health, its subsidiaries or affiliates. This document may also contain information covered under the Health Insurance Portability and Accountability Act (HIPAA, PL 104-191) and implementing regulations and must be protected in accordance with those provisions. Re-disclosure without patient consent or as otherwise permitted by law is prohibited. Any unauthorized review, retransmission, use, printing, copying, retention, disclosure, distribution or the taking of any action in reliance upon this information by persons or entities other than the intended recipient is strictly prohibited and may be unlawful. If you have received this message in error, please immediately advise the sender by reply email message to the sender and delete all copies of this message from your system without copying. ________________________________ From LNormington at uwhealth.org Tue Mar 1 14:43:37 2016 From: LNormington at uwhealth.org (Normington Lacy) Date: Tue, 1 Mar 2016 20:43:37 +0000 Subject: [Histonet] Histology Positions at UW Health Madison Message-ID: <7F7174244DDD1A49BFBEE8A845BC2F2B18634F@UWHC-MBX05.uwhis.hosp.wisc.edu> UW Health, located in Madison Wisconsin, is looking to hire multiple histology technicians. UW Health offers a competitive compensation and benefits package. Work experience that is relevant to the position will be taken into consideration when determining the starting base pay. Please log onto www.uwhealth.org to apply. Below are the positions posted. 160020TH 0.5 FTE Monday through Friday 5:00am - 9:00am 160020TG 1.0 FTE Monday through Friday 8:30am - 5:00pm 150004HF 1.0 FTE Monday through Friday 8:30am - 5:00pm, primarily working in IHC 16002044 0.8 FTE Monday through Wednesday 10:30am - 5:00pm, Thursday through Friday 9:30 - 5:00pm Lacy Normington, HTL(ASCP)CM Manager, Surgical Pathology Lab Services UWHospital and Clinics 600 Highland Avenue Madison, WI 53792-2472 Phone: 608-890-9373 From mjones at metropath.com Tue Mar 1 15:14:20 2016 From: mjones at metropath.com (Michael Ann Jones) Date: Tue, 1 Mar 2016 21:14:20 +0000 Subject: [Histonet] Freeze Spary Ban continued In-Reply-To: <648AE493-95B3-44C0-8C64-2FEC2BD8CB61@cchmc.org> References: <09CFA3F99D5B2B42B88CDFB2FC4CFD823458AA@vdc01.domain.local> <648AE493-95B3-44C0-8C64-2FEC2BD8CB61@cchmc.org> Message-ID: So does Statlab?s Freeze spray. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com On 3/1/16, 11:13 AM, "Macke, Gail via Histonet" wrote: >FYI: >Just looked at Leica/Surgipath?s Frostbite and it has the 1,1,1,2 >Tetrafluoroethane in it ,too. > >Gail Macke, >Cincinnati Childrens Hospital Medical Center > > >On Mar 1, 2016, at 12:15 PM, Histology via Histonet >u>> wrote: > >FYI: > >Mercedes Medical DOES contain the banned chemical. > >StatLab's 1,1,1,2 Tetrafluoroethane is banned. This is also called HFC >134a > >Still checking other brands. > >-Mehndi > > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu> >https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.e >du_mailman_listinfo_histonet&d=BQICAg&c=P0c35rBvlN7D8BNx7kSJTg&r=EHoZsUPFU >92qg6yiYwM5gy-CtQoI_1E8mAl9MzLu1wM&m=8Bqfye7yLupm62E4ro4YiQmrQ7iVpvaFhaBHI >Kn8gKw&s=kKwXSH1ofdUzWU2zcVaXZgqNOlLGdRM112PF0SsbW9k&e= > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From George.John at UTSouthwestern.edu Tue Mar 1 15:44:32 2016 From: George.John at UTSouthwestern.edu (George John) Date: Tue, 1 Mar 2016 21:44:32 +0000 Subject: [Histonet] automatic stainers and tissue processors In-Reply-To: References: Message-ID: <82FA35DE3A1BD546BA2C7353A55E970418E9E643@swmsmail9.swmed.org> I would recommend Peloris Tissue Processor and Leica ST5020 HE Stainer. The HE600 Stainer is superior quality but is much more expensive. George B John PhD, HTL(ASCP), QIHC Supervisor, Histology, IHC, NP, EM Clinical Laboratory Services Room No CUH04.254 Tel- 214 633 6359/6442, Pager: 214 786 9398 email- george.john at utsouthwestern.edu -----Original Message----- From: Jamal Rowaihi [mailto:j.rowaihi at alborglaboratories.com] Sent: Monday, February 29, 2016 3:24 PM To: Silvia Bonner ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] automatic stainers and tissue processors Go for Sakura products with no hesitate. Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent from my cell phone-------- Original message --------From: Silvia Bonner via Histonet Date: 2/29/2016 11:09 PM (GMT+03:00) To: histonet at lists.utsouthwestern.edu Subject: [Histonet] automatic stainers and tissue processors Hello, We are looking into purchasing new automatic H&E strainers and new tissue processors. Any helpful advice? I know this may have been discussed before but I an new to histonet. Thanks for your help! Silvia Bonner, HT(ASCP) CM Histology Supervisor sbonner at pathregional.com Pathologists' Regional Laboratory 1225 Highland Ave Clarkston, WA 99403 This email may contain physician, patient and/or attorney material that is confidential or privileged for the sole use of the intended recipient. YOU ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED. If you are not the intended recipient, please notify us immediately by telephone at (208) 746-0516 or (509) 758-5576 and delete all copies. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ UT Southwestern Medical Center The future of medicine, today. From ctorrence at kmcpa.com Tue Mar 1 17:38:09 2016 From: ctorrence at kmcpa.com (Carol Torrence) Date: Tue, 1 Mar 2016 23:38:09 +0000 Subject: [Histonet] AP Easy Pathology reporting system and adding slide/cassette Printers Message-ID: We are looking into adding a cassette and slide labeling system and would like to poll the masses :) If you use slide/cassette printers, I would like your 2 cents about your current brand. Pros and cons. If you use AP Easy and have joined a printer to the software, I would also be curious about your experience with the model that you use. I am impressed with General Data however cassette and label costs seem like a costly commitment. Thanks in advance! Carol, from the Land of Oz From mills at 3scan.com Tue Mar 1 18:20:43 2016 From: mills at 3scan.com (Caroline Miller) Date: Tue, 1 Mar 2016 16:20:43 -0800 Subject: [Histonet] Resin processing gone crystally! Message-ID: HI All, I know we are mainly paraffin folks out there, but I have a resin question and I hope someone out there can help us. We process large volumes of tissue to LR white and have had no issues in the past, however this last batch went very weird. We have been doing this for over a year with no issues but after one night in 50% resin 50% alcohol the resin seemed to precipitate out into white solid crystals totally replacing the liquid resin. Has anyone had this happen before? We would really like it not to happen again as it seems to be also affecting the tissue too! help please! I am hoping you can see these, and therefore I got the sharing settings right: https://drive.google.com/drive/u/0/folders/0B0bZ0Efb6jfBZEZ6RmhSRkhDbkU and https://drive.google.com/a/3scan.com/folderview?id=0B0bZ0Efb6jfBZEZ6RmhSRkhDbkU&usp=sharing_eid&ts=56d6234c Also a fuzzy picture of the crystalized resin (i was not responsible for the fuzziness, but I work with what I got)! Thanks in advance for all your wonderful advice! yours, mills -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From relia1 at earthlink.net Wed Mar 2 09:03:57 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 2 Mar 2016 10:03:57 -0500 Subject: [Histonet] Histotechnology Professionals Day is March 10. What are you doing to celebrate? Need Ideas? Read on! Message-ID: <000001d17494$bf19dca0$3d4d95e0$@earthlink.net> Hi Histonetters!! Did you know that March 10 is Histotechnology Professionals Day? I want to thank you not only for the job that you do and the lives that you save but also for allowing me to work with you in improving your careers. In all my years of recruiting I have never worked with a more professional, appreciative, honest and truly enjoyable group of people. Kudos to you!! The National Society for Histotechnology has some great suggestions for ways to celebrate. Here is the link: http://www.nsh.org/content/histotechnology-professionals-day What are you doing to celebrate? In honor of Histotechnology Professionals Day RELIA Solutions celebrates 4 years as a Sustaining member of the National Society of Histotechnology. I have amazing opportunities with outstanding clients that I want to tell you about- here is a quick list of the positions I am most excited about. All of these are full time permanent opportunities and our clients offer excellent compensation, benefits and in most cases relo/sign on bonuses. Here is the list of my HOTTEST OPPORTUNITIES !! Histology Leadership Opportunities: Histology Supervisor ? Amarillo, TX Lead Histotechnologist/Supervisor ? Great Falls, MT Clinical Lab Specialist ? Virginia Beach, VA Lead Histotechnologist/Supervisor ? Fayetteville, AR IHC Specialist ? Long Island, NY Histology Technician/Technologist Norfolk, VA 15K sign on bonus. NEW OPENING DUE TO GROWTH! Long Island, NY NYS License required; great team to work with. Long Island, NY IHC Specialist; NYS license required. Tyler, TX ? Learn Mohs. Dallas, TX Days; opportunity for advancement. Little Rock, AR State of the art lab; Junior techs welcome to apply. Flagstaff, AZ Beautiful area; Day shift. Louisville, KY Learn Electron Microscopy. Milwaukee, WI ? Days; fast paced lab, great team to work with. Is Histotechnology Professionals Day a good time to reflect on your career, where you are, what you want, and how are you going to get there? If you aren?t considering a job change: Is it time to join/renew your NSH membership? Check out your state society? Join the Histonet? Take some CEUs to stay up to date and learn some new skills? Get that state license for the place you want to move to one day? Start studying for that QIHC? If you are or might be considering a job change now or in the near future: Does your resume need to be updated? Have you considered what you are looking for and where? Have you gotten the state license if it is required before you go? Have we spoken recently? Please feel free to contact me by phone toll free at 866-607-3542 or email: relia1 at earthlink.net if you want information or help with any of the items listed. My services are always free of charge to you. If you have any friends that might benefit from receiving this please feel free to pass it along. Don?t forget to shoot me back an email to share what you did for Histotechnology Professionals Day. I really want to know ? Have A Happy Histo Day!! >Pam Thanks-Pam Barker - President RELIA Solutions Right Place, Right Time, Right Move with RELIA! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Richard.Cartun at hhchealth.org Wed Mar 2 12:19:56 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Wed, 2 Mar 2016 18:19:56 +0000 Subject: [Histonet] Slide scribe Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B0B4A3F@HHCEXCHMB03.hhcsystem.org> Years ago, I obtained a metal scribe for etching glass slides, but I can't remember which Histology company I got it from. Any suggestions? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From marktarango at gmail.com Wed Mar 2 12:38:01 2016 From: marktarango at gmail.com (Mark Tarango) Date: Wed, 2 Mar 2016 10:38:01 -0800 Subject: [Histonet] Slide scribe In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E6B0B4A3F@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E6B0B4A3F@HHCEXCHMB03.hhcsystem.org> Message-ID: You could try these from Ted Pella: http://www.tedpella.com/glasswar_html/scriber.htm On Wed, Mar 2, 2016 at 10:19 AM, Cartun, Richard via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Years ago, I obtained a metal scribe for etching glass slides, but I can't > remember which Histology company I got it from. Any suggestions? Thank > you. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & > Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From modz9636 at gmail.com Wed Mar 2 13:36:27 2016 From: modz9636 at gmail.com (M.O.) Date: Wed, 2 Mar 2016 11:36:27 -0800 Subject: [Histonet] cryosectioning undecalcified samples Message-ID: Hello, I am looking into options for cryosectioning undecalcified mouse knees. One of my co-workers said he used a film in Japan that stuck to the cryoblock and then would do IHC directly on this tape. Has anyone cut undecalcified cryosections? If so, what options are there and do you recommend one over the other? Thank you, Merissa From sbaldwin at mhhcc.org Wed Mar 2 14:21:00 2016 From: sbaldwin at mhhcc.org (Baldwin, Kathy) Date: Wed, 2 Mar 2016 20:21:00 +0000 Subject: [Histonet] Cytology slide time documentation Message-ID: <1f3c812479e14647a96ab45c908b04a5@exch02.mhhcc.org> Hi all Just wondering if there are any cytologists out in Histo land that would share with me there slide time documentation. We are currently having to fill out 2 papers and it is awfully cumbersome. Any help ?? Thanks S. Kathy Baldwin Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Briana.Zeck at nyumc.org Wed Mar 2 16:41:40 2016 From: Briana.Zeck at nyumc.org (Briana Zeck) Date: Wed, 2 Mar 2016 22:41:40 +0000 Subject: [Histonet] Leica Reichert-Jung Biocut 2030 Microtome Manual Message-ID: I was wondering if anyone has a copy of the manual for the Leica Reichert-Jung Biocut 2030 Microtome. We are looking to take ours apart for cleaning and maintenance, but are hesitant to do so without having the manual on hand. If you do have this microtome but don't have the manual handy, would you mind sharing what your protocols for preventative maintenance are? Thanks for your time! Briana L. Zeck Immunohistochemistry Core NYULMC Office of Collaborative Science (OCS) New York, NY 10016 ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= From cjohnson at nmda.nmsu.edu Thu Mar 3 12:58:52 2016 From: cjohnson at nmda.nmsu.edu (Johnson, Carole) Date: Thu, 3 Mar 2016 18:58:52 +0000 Subject: [Histonet] BioCare Mouse-on-Farma AP-Polymer? Message-ID: Hello and Happy (almost) Friday, I'm behind the curve, I'll admit it! If you have had experience with BioCare's farma detection, , (or canine/feline), I would appreciate your input. I'm staining porcine brain using Refine Red (Leica) with good results but would like to decrease my background even further (a less expensive reagent would also be a plus!). Also considering trying the canine/feline product for my routine testing if the farma works well. Thanks! Carole L. Johnson, HT(ASCP)cm, QIHC New Mexico Department of Agriculture Veterinary Diagnositc Services 1101 Camino de Salud, NE Albuquerque, NM 87101 505.383.9299 Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From Elise_ODea at URMC.Rochester.edu Thu Mar 3 13:00:47 2016 From: Elise_ODea at URMC.Rochester.edu (ODea, Elise) Date: Thu, 3 Mar 2016 19:00:47 +0000 Subject: [Histonet] Fluids for Cytology Message-ID: <60358e9c7227404da76c11b472775feb@exmbxpdc06.urmc-sh.rochester.edu> Question: I am wondering whose responsibility is it to process and prepare cytological fluids in a 260 bed hospital w/o a cytology tech.? Lend me your thoughts... Elise Elise T. O'Dea Histology Section Head Highland Hospital 1000 South Ave. Rochester, New York 585.341.6596 585.341.8314 elise_odea at urmc.rochester.edu -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, March 03, 2016 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 148, Issue 3 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=pSEtIqYqX9Bos4EXIqncXiqc4w83FTqEqBRx7VhWaFc&m=4lhe5__Rf7fgowzK4xv8k8hxqENfupEfKCAwPwi3cYw&s=hlybgv9oPVhNX_M8R8HcpZRqewQXRehdR6OFtqcslcU&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Slide scribe (Cartun, Richard) 2. Re: Slide scribe (Mark Tarango) 3. cryosectioning undecalcified samples (M.O.) 4. Cytology slide time documentation (Baldwin, Kathy) 5. Leica Reichert-Jung Biocut 2030 Microtome Manual (Briana Zeck) ---------------------------------------------------------------------- Message: 1 Date: Wed, 2 Mar 2016 18:19:56 +0000 From: "Cartun, Richard" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Slide scribe Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B0B4A3F at HHCEXCHMB03.hhcsystem.org> Content-Type: text/plain; charset="us-ascii" Years ago, I obtained a metal scribe for etching glass slides, but I can't remember which Histology company I got it from. Any suggestions? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ Message: 2 Date: Wed, 2 Mar 2016 10:38:01 -0800 From: Mark Tarango To: "Cartun, Richard" Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Slide scribe Message-ID: Content-Type: text/plain; charset=UTF-8 You could try these from Ted Pella: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.tedpella.com_glasswar-5Fhtml_scriber.htm&d=BQICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=pSEtIqYqX9Bos4EXIqncXiqc4w83FTqEqBRx7VhWaFc&m=4lhe5__Rf7fgowzK4xv8k8hxqENfupEfKCAwPwi3cYw&s=6iO9IsZqlNGt4fl7SfHWKEMlHHiEj0o2w0iwwxu9SYM&e= On Wed, Mar 2, 2016 at 10:19 AM, Cartun, Richard via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Years ago, I obtained a metal scribe for etching glass slides, but I > can't remember which Histology company I got it from. Any > suggestions? Thank you. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & > Morphologic Proteomics Laboratory Director, Biospecimen Collection > Programs Assistant Director, Anatomic Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=pSEtIqYqX9Bos4EXIqncXiqc4w83FTqEqBRx7VhWaFc&m=4lhe5__Rf7fgowzK4xv8k8hxqENfupEfKCAwPwi3cYw&s=hlybgv9oPVhNX_M8R8HcpZRqewQXRehdR6OFtqcslcU&e= > ------------------------------ Message: 3 Date: Wed, 2 Mar 2016 11:36:27 -0800 From: "M.O." To: Histonet Subject: [Histonet] cryosectioning undecalcified samples Message-ID: Content-Type: text/plain; charset=UTF-8 Hello, I am looking into options for cryosectioning undecalcified mouse knees. One of my co-workers said he used a film in Japan that stuck to the cryoblock and then would do IHC directly on this tape. Has anyone cut undecalcified cryosections? If so, what options are there and do you recommend one over the other? Thank you, Merissa ------------------------------ Message: 4 Date: Wed, 2 Mar 2016 20:21:00 +0000 From: "Baldwin, Kathy" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Cytology slide time documentation Message-ID: <1f3c812479e14647a96ab45c908b04a5 at exch02.mhhcc.org> Content-Type: text/plain; charset="us-ascii" Hi all Just wondering if there are any cytologists out in Histo land that would share with me there slide time documentation. We are currently having to fill out 2 papers and it is awfully cumbersome. Any help ?? Thanks S. Kathy Baldwin Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 5 Date: Wed, 2 Mar 2016 22:41:40 +0000 From: Briana Zeck To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Leica Reichert-Jung Biocut 2030 Microtome Manual Message-ID: Content-Type: text/plain; charset="us-ascii" I was wondering if anyone has a copy of the manual for the Leica Reichert-Jung Biocut 2030 Microtome. We are looking to take ours apart for cleaning and maintenance, but are hesitant to do so without having the manual on hand. If you do have this microtome but don't have the manual handy, would you mind sharing what your protocols for preventative maintenance are? Thanks for your time! Briana L. Zeck Immunohistochemistry Core NYULMC Office of Collaborative Science (OCS) New York, NY 10016 ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=pSEtIqYqX9Bos4EXIqncXiqc4w83FTqEqBRx7VhWaFc&m=4lhe5__Rf7fgowzK4xv8k8hxqENfupEfKCAwPwi3cYw&s=hlybgv9oPVhNX_M8R8HcpZRqewQXRehdR6OFtqcslcU&e= ------------------------------ End of Histonet Digest, Vol 148, Issue 3 **************************************** From mills at 3scan.com Thu Mar 3 13:45:30 2016 From: mills at 3scan.com (Caroline Miller) Date: Thu, 3 Mar 2016 11:45:30 -0800 Subject: [Histonet] cryosectioning undecalcified samples In-Reply-To: References: Message-ID: In this space there is the cryojane (expensive, http://www.leicabiosystems.com/research/neuroscience/details/product/tape-transfer-system/) or the Japanese Kawamoto method: http://section-lab.jp/English/Introduction.htm I have also found that if you take a regular piece of scotch tape and put it on the block prior to sectioning that it also works :) (trim to your area of interest, move the blade along, place on the tape and press down (use a tool to do this - don't use your hand to reduce the warming of the tissue, which would give too big a slice), then flip the scotch tape to the outside of the blade and take a section. You can then stain the tissue on the tape, dehydrate and clear, then stick the whole thing under a coverslip. Crude but effective for standard stains. I could not get immunostaining to work with it though...... It is hard to then get the tissue off the tape - apparently it works with hexane for the Kawamoto method, but I could never get that to work Ultimately if you were going to buy something I would say the Kawamoto is a great place to start, they (he) has a bunch of good tools in cute boxes that you can work with to make the process easier Good Luck! mills On Wed, Mar 2, 2016 at 11:36 AM, M.O. via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello, > > I am looking into options for cryosectioning undecalcified mouse knees. > One of my co-workers said he used a film in Japan that stuck to the > cryoblock and then would do IHC directly on this tape. > > Has anyone cut undecalcified cryosections? If so, what options are there > and do you recommend one over the other? > > Thank you, > Merissa > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From cdemarinis at SARATOGACARE.ORG Thu Mar 3 13:50:10 2016 From: cdemarinis at SARATOGACARE.ORG (Demarinis, Carolyn) Date: Thu, 3 Mar 2016 19:50:10 +0000 Subject: [Histonet] B-Plus Fix Message-ID: <1C75A843982A7B44BB368A3CC946ABCA012D5BC0AD@SHEXCHMBX01.SARAHOSP.ORG> I am considering using B-Plus fix for our bone marrow biopsies in place of Bouin's fixative and I would like feedback. Please let me know your procedure for bone marrow biopsies and if it is necessary to decalcify the bone marrow biopsies following the B-Plus fix. Thank you. -- This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy at saratogacare.org and destroy all copies of this communication and any attachments. From Valerie.Hannen at parrishmed.com Fri Mar 4 04:56:27 2016 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Fri, 4 Mar 2016 05:56:27 -0500 Subject: [Histonet] Fluids for Cytology In-Reply-To: <60358e9c7227404da76c11b472775feb@exmbxpdc06.urmc-sh.rochester.edu> References: <60358e9c7227404da76c11b472775feb@exmbxpdc06.urmc-sh.rochester.edu> Message-ID: <450B7A81EDA0C54E97C53D60F00776C323546750C6@isexstore03> We in Histology do the processing and preparation of the fluids. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: ODea, Elise via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, March 03, 2016 2:01 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Fluids for Cytology Question: I am wondering whose responsibility is it to process and prepare cytological fluids in a 260 bed hospital w/o a cytology tech.? Lend me your thoughts... Elise Elise T. O'Dea Histology Section Head Highland Hospital 1000 South Ave. Rochester, New York 585.341.6596 585.341.8314 elise_odea at urmc.rochester.edu -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, March 03, 2016 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 148, Issue 3 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=pSEtIqYqX9Bos4EXIqncXiqc4w83FTqEqBRx7VhWaFc&m=4lhe5__Rf7fgowzK4xv8k8hxqENfupEfKCAwPwi3cYw&s=hlybgv9oPVhNX_M8R8HcpZRqewQXRehdR6OFtqcslcU&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Slide scribe (Cartun, Richard) 2. Re: Slide scribe (Mark Tarango) 3. cryosectioning undecalcified samples (M.O.) 4. Cytology slide time documentation (Baldwin, Kathy) 5. Leica Reichert-Jung Biocut 2030 Microtome Manual (Briana Zeck) ---------------------------------------------------------------------- Message: 1 Date: Wed, 2 Mar 2016 18:19:56 +0000 From: "Cartun, Richard" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Slide scribe Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B0B4A3F at HHCEXCHMB03.hhcsystem.org> Content-Type: text/plain; charset="us-ascii" Years ago, I obtained a metal scribe for etching glass slides, but I can't remember which Histology company I got it from. Any suggestions? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ Message: 2 Date: Wed, 2 Mar 2016 10:38:01 -0800 From: Mark Tarango To: "Cartun, Richard" Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Slide scribe Message-ID: Content-Type: text/plain; charset=UTF-8 You could try these from Ted Pella: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.tedpella.com_glasswar-5Fhtml_scriber.htm&d=BQICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=pSEtIqYqX9Bos4EXIqncXiqc4w83FTqEqBRx7VhWaFc&m=4lhe5__Rf7fgowzK4xv8k8hxqENfupEfKCAwPwi3cYw&s=6iO9IsZqlNGt4fl7SfHWKEMlHHiEj0o2w0iwwxu9SYM&e= On Wed, Mar 2, 2016 at 10:19 AM, Cartun, Richard via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Years ago, I obtained a metal scribe for etching glass slides, but I > can't remember which Histology company I got it from. Any > suggestions? Thank you. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & > Morphologic Proteomics Laboratory Director, Biospecimen Collection > Programs Assistant Director, Anatomic Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution > is prohibited. If you are not the intended recipient, or an employee > or agent responsible for delivering the message to the intended > recipient, please contact the sender by reply e-mail and destroy all > copies of the original message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=BQICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSn > M3bY53YSKuLUQRxhA&r=pSEtIqYqX9Bos4EXIqncXiqc4w83FTqEqBRx7VhWaFc&m=4lhe > 5__Rf7fgowzK4xv8k8hxqENfupEfKCAwPwi3cYw&s=hlybgv9oPVhNX_M8R8HcpZRqewQX > RehdR6OFtqcslcU&e= > ------------------------------ Message: 3 Date: Wed, 2 Mar 2016 11:36:27 -0800 From: "M.O." To: Histonet Subject: [Histonet] cryosectioning undecalcified samples Message-ID: Content-Type: text/plain; charset=UTF-8 Hello, I am looking into options for cryosectioning undecalcified mouse knees. One of my co-workers said he used a film in Japan that stuck to the cryoblock and then would do IHC directly on this tape. Has anyone cut undecalcified cryosections? If so, what options are there and do you recommend one over the other? Thank you, Merissa ------------------------------ Message: 4 Date: Wed, 2 Mar 2016 20:21:00 +0000 From: "Baldwin, Kathy" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Cytology slide time documentation Message-ID: <1f3c812479e14647a96ab45c908b04a5 at exch02.mhhcc.org> Content-Type: text/plain; charset="us-ascii" Hi all Just wondering if there are any cytologists out in Histo land that would share with me there slide time documentation. We are currently having to fill out 2 papers and it is awfully cumbersome. Any help ?? Thanks S. Kathy Baldwin Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 5 Date: Wed, 2 Mar 2016 22:41:40 +0000 From: Briana Zeck To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Leica Reichert-Jung Biocut 2030 Microtome Manual Message-ID: Content-Type: text/plain; charset="us-ascii" I was wondering if anyone has a copy of the manual for the Leica Reichert-Jung Biocut 2030 Microtome. We are looking to take ours apart for cleaning and maintenance, but are hesitant to do so without having the manual on hand. If you do have this microtome but don't have the manual handy, would you mind sharing what your protocols for preventative maintenance are? Thanks for your time! Briana L. Zeck Immunohistochemistry Core NYULMC Office of Collaborative Science (OCS) New York, NY 10016 ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=pSEtIqYqX9Bos4EXIqncXiqc4w83FTqEqBRx7VhWaFc&m=4lhe5__Rf7fgowzK4xv8k8hxqENfupEfKCAwPwi3cYw&s=hlybgv9oPVhNX_M8R8HcpZRqewQXRehdR6OFtqcslcU&e= ------------------------------ End of Histonet Digest, Vol 148, Issue 3 **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From criley at dpspa.com Fri Mar 4 05:36:17 2016 From: criley at dpspa.com (Charles Riley) Date: Fri, 4 Mar 2016 06:36:17 -0500 Subject: [Histonet] Cell block protocols Message-ID: Hello all, Our pathologists are worried about our cell yield with our cell blocks. Can anyone share their protocols and what you use to make the cell blocks. I do not know what our protocol is as they are made by the second shift cytoprep team and I work the early shift -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE From LRaff at uropartners.com Fri Mar 4 12:22:11 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 4 Mar 2016 18:22:11 +0000 Subject: [Histonet] (Lab related) Blog Post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B71A8AF@COLOEXCH01.uropartners.local> This is more for the lay public-but you may want to add in your own inspection experiences. http://www.chicagonow.com/downsize-maybe/2016/03/testing-the-tester-lab-inspections-101/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From tbraud at holyredeemer.com Fri Mar 4 13:18:59 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 4 Mar 2016 19:18:59 +0000 Subject: [Histonet] Processing cytology and cell block protocols Message-ID: <48E053DDF6CE074DB6A7414BA05403F80643E0@HRHEX02-HOS.holyredeemer.local> We are a similar sized hospital with an 8,000/yr Surgical load of mixed large and small cases. We process 1000 Non-gyn Cytology cases, assist with FNA collection in Interventional Radiology. We also assist with about 130 bone marrow collections, including smears and processing. There is myself, and 3 other Histotechs and we do it all! Our cell block protocol for unfixed fluids is Centrifuge in 50ml conical tubes at 1200rpm for 10 minutes Pour off supernate, resuspend the pellet in buffer wash (for large volumes with low cell yields, we keep spinning the fluid until we get a cell pellet in the bottom of the tube, or we have exhausted the fluid) Repeat centrifuge step Pour off supernate and fix in 10%NBF/95% Alcohol 50/50 for at least 10 minutes Gently dislodge the fixed pellet and pour into the corner of a nylon bag into a cassette for routine processing If the fixed pellet is smaller than 0.5cm in greatest dimension, then we pour off the fixative and add heated to liquid agar Let cool, then gently dislodge the fixed agar pellet and place gently into the corner of a nylon bag into a cassette for routine processing Hope this helps Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Friday, March 04, 2016 1:00 PM Today's Topics: 2. Re: Fluids for Cytology 6. Cell block protocols * From abtdhu at gmail.com Fri Mar 4 13:20:53 2016 From: abtdhu at gmail.com (Dorothy Hu) Date: Fri, 4 Mar 2016 14:20:53 -0500 Subject: [Histonet] cryosectioning undecalcified samples (Caroline Miller) Message-ID: Hi, I compared cryojane with Kawamoto's method. I prefer the later. Please see the following paper. Arch Histol Cytol. 2003 May;66(2):123-43. Use of a new adhesive film for the preparation of multi-purpose fresh-frozen sections from hard tissues, whole-animals, insects and plants. Kawamoto T 1. Good luck. Dorothy From Linda.Margraf at cookchildrens.org Fri Mar 4 13:21:56 2016 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Fri, 4 Mar 2016 19:21:56 +0000 Subject: [Histonet] Helpful Histonet hints Message-ID: <049002027f014e96825c8d87462855c4@MBX11.CCHCS.LDAP> Dear Histonetters, I get a lot of queries from members of the list who try to post a message and receive a note from the server that they are not members and their message is being held. This problem usually happens when someone's email address has changed since they first subscribed. Any sort of trivial change to an address will cause the server to block posting messages from that member even though they still receive messages just fine as they are internally routed through their email system. If this happens to you, please go to the Histonet website: http://lists.utsouthwestern.edu/mailman/listinfo/histonet and put your old address in at the bottom in the area for subscribers. You can edit your address there and also switch to digest mode (receive all mail in one mailing once a day) if you'd prefer. Let me know if you need help. The other reason messages are sometimes not posted is if you have graphics in your message (especially logos etc. in the signature line). The server often rejects those and I cannot predict when that will happen. If your message doesn't get posted and you don't know why, try sending it as plain text (which removes the graphics). "Plain text" will probably be in the options or format text area in your email system header. Please let me know if you have problems using the histonet-owner at lists.utsouthwestern.edu link. Thanks Linda M Histonet administrator From rsrichmond at gmail.com Fri Mar 4 13:29:41 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 4 Mar 2016 14:29:41 -0500 Subject: [Histonet] B-Plus fixative Message-ID: Carolyn Demarinis at Saratoga Springs NY asks: >>I am considering using B-Plus fix for our bone marrow biopsies in place of Bouin's fixative and I would like feedback. Please let me know your procedure for bone marrow biopsies and if it is necessary to decalcify the bone marrow biopsies following the B-Plus fix.<< There really isn't any bone marrow fixative you can use today that's significantly better than neutral buffered formalin. Adequate fixation and properly controlled decalcification are essential, and result in a day's delay if the specimen doesn't get in fairly early in the morning. B-Plus fixed specimens require decalcification. You really can't combine fixation and decalcification in a single step. Bob Richmond Samurai Pathologist Maryville TN From James.E.Nutter at questdiagnostics.com Fri Mar 4 13:56:34 2016 From: James.E.Nutter at questdiagnostics.com (Nutter, James E) Date: Fri, 4 Mar 2016 19:56:34 +0000 Subject: [Histonet] Thermoelectric Cold Plates Message-ID: <0C8F16C2CB837745AE0AB3F20E4F6FB461966513@qdcws1640.us.qdx.com> I have heard of people using old embedding center cold plates but there is something a little newer out there. Has anyone tried using thermoelectric cold plates instead of ice to cool blocks for cutting? Did it work? If so do you have a vendor? Thank you, James Nutter Supervisor Histology Quest Diagnostics | Action from Insight | 14225 Newbrook Dr. | Chantilly, VA 20151 USA | phone 703.802.6900 x65753 | fax 703.802.7191 | James.E.Nutter at QuestDiagnostics.com | QuestDiagnostics.com ______________________________________________________________________ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. From jshelley at sbpdiscovery.org Mon Mar 7 08:38:19 2016 From: jshelley at sbpdiscovery.org (John Shelley) Date: Mon, 7 Mar 2016 14:38:19 +0000 Subject: [Histonet] Florida Society for Histotechnology Spring Meeting Message-ID: Hi All, Happy Monday! I would like to inform all histonetters both in Florida and abroad that our meeting program has been finalized and registration is now open. We have a wonderful array of workshops that will appeal to all levels of experience. We have our 3rd annual All-Day Supervisor and Administrator Forum on Friday, May 20th, 2016 along with our All-Day HT Preparedness Workshop for students and those who are looking for a fresher on Saturday, May 21st, 2016. Along with general histology, safety, IHC, molecular, digital imaging and much more. Please use links below to look at our program and then our links for registration to the meeting and hotel. FSH Online Registration FSH Online Program FSH Hotel Registration Look forward to seeing you all in sunny and warm St. Pete., Florida at the Bayfront Hilton on May 19-22, 2016. Kind Regards! John J Shelley 2014-2016 FSH President Histology Core Manager Sanford Burnham Prebys Medical Discovery Institute at Lake Nona From LRaff at uropartners.com Mon Mar 7 09:21:01 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 7 Mar 2016 15:21:01 +0000 Subject: [Histonet] Blog post--lab related Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B72E334@COLOEXCH01.uropartners.local> http://www.chicagonow.com/downsize-maybe/2016/03/no-lovie-for-our-lab/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From Kjones at upei.ca Mon Mar 7 10:16:53 2016 From: Kjones at upei.ca (Kathleen Jones) Date: Mon, 07 Mar 2016 12:16:53 -0400 Subject: [Histonet] Whale skin Message-ID: <56DD71350200008B0006DE01@oes-grpwise.novell.upei.ca> Hello, I have to cut FFPE blocks of whale skin for in IHC study. I am having difficulty with both cutting and floating. Any advice would be much appreciated! Thank you! Kathleen Jones Research Technologist Pathology/Microbiology AVC - UPEI From rjbuesa at yahoo.com Mon Mar 7 10:57:23 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 7 Mar 2016 16:57:23 +0000 (UTC) Subject: [Histonet] Whale skin In-Reply-To: <56DD71350200008B0006DE01@oes-grpwise.novell.upei.ca> References: <56DD71350200008B0006DE01@oes-grpwise.novell.upei.ca> Message-ID: <914914500.3949222.1457369843481.JavaMail.yahoo@mail.yahoo.com> How did you manage to deal with the about 0.5 m of blubber?Was it the skin of a new born whale? I just to not understand, but you have to completely eliminate all the fat and increase your processing protocol (infiltration specially) to have some chance of getting any relatively "decent" section.Ren? On Monday, March 7, 2016 11:23 AM, Kathleen Jones via Histonet wrote: Hello, I have to cut FFPE blocks of whale skin for in IHC study. I am having difficulty with both cutting and floating. Any advice would be much appreciated! Thank you! Kathleen Jones Research Technologist Pathology/Microbiology AVC - UPEI _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at dpspa.com Mon Mar 7 11:50:51 2016 From: criley at dpspa.com (Charles Riley) Date: Mon, 7 Mar 2016 12:50:51 -0500 Subject: [Histonet] DI water Message-ID: Does anyone know of a cheap way to make your own DI water? Or a good reliable company in the Delaware area that provides DI water services? -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE From jvickroy at SpringfieldClinic.com Mon Mar 7 12:32:55 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Mon, 7 Mar 2016 18:32:55 +0000 Subject: [Histonet] Microscopic slides Message-ID: <9B1A1501A800064397369BD8072E6BCA065167C2@E2K10DB.springfieldclinic.com> We are once again reviewing the microscopic slides we use. We have found the sldies we have used on a regular basis take too long to dry properly which gives us some undesireable artifacts. I have longed for a "one size fits all" microscopic slide. One of the slides I used in the past is not possible given that I have the Thermofisher Slidemates. The slidemates require a very smooth labeled end. Recently we tested several slides both hydrophobic and hydrophilic. We really liked a hydrophilic slide by Leica but am told it doesn't work well for IHC's and special stains. Our current slide is a "hydrophobic" slide which traps water easily underneath the section and therefore we having drying problems. If we don't dry them for at least twenty minutes we get nuclear bubbling. Leica has another slide we tried that is "hydrophobic" but with "hydrophilic" tendencies. My staff thought tried that slide and found them to be very similar to the "hydrophobic" slide we already have. We have tried the Stat-lab M2000 and they seem to dry better but there often is a "trick" to getting the sections to stick to the slide without sliding off of the end. Of course we might just have to use two different slides, one for our routine H&E's and one for the IHC's and Special stains. Anybody have any thoughts on this? And is a "hydrophobic slide" that has "hydrophilic tendencies" really possible? Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From wbenton at cua.md Mon Mar 7 12:53:45 2016 From: wbenton at cua.md (Walter Benton) Date: Mon, 7 Mar 2016 18:53:45 +0000 Subject: [Histonet] Microscopic slides In-Reply-To: <9B1A1501A800064397369BD8072E6BCA065167C2@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA065167C2@E2K10DB.springfieldclinic.com> Message-ID: <6b6f84d430654669917ec5f124b43837@MAIL01.GCU-MD.local> Try Globe and Tanner slides, both should work well in your slidemate. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: Vickroy, James via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, March 07, 2016 1:33 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Microscopic slides We are once again reviewing the microscopic slides we use. We have found the sldies we have used on a regular basis take too long to dry properly which gives us some undesireable artifacts. I have longed for a "one size fits all" microscopic slide. One of the slides I used in the past is not possible given that I have the Thermofisher Slidemates. The slidemates require a very smooth labeled end. Recently we tested several slides both hydrophobic and hydrophilic. We really liked a hydrophilic slide by Leica but am told it doesn't work well for IHC's and special stains. Our current slide is a "hydrophobic" slide which traps water easily underneath the section and therefore we having drying problems. If we don't dry them for at least twenty minutes we get nuclear bubbling. Leica has another slide we tried that is "hydrophobic" but with "hydrophilic" tendencies. My staff thought tried that slide and found them to be very similar to the "hydrophobic" slide we already have. We have tried the Stat-lab M2000 and they seem to dry better but there often is a "trick" to getting the sections to stick to the slide without sliding off of the end. Of course we might just have to use two different slides, one for our routine H&E's and one for the IHC's and Special stains. Anybody have any thoughts on this? And is a "hydrophobic slide" that has "hydrophilic tendencies" really possible? Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From mills at 3scan.com Mon Mar 7 13:15:52 2016 From: mills at 3scan.com (Caroline Miller) Date: Mon, 7 Mar 2016 11:15:52 -0800 Subject: [Histonet] DI water In-Reply-To: References: Message-ID: We use this one: http://smile.amazon.com/Pure-Stainless-Counter-Distiller-Secure/dp/B003ZJRAWO/ref=pd_sim_sbs_60_12?ie=UTF8&refRID=1A4WVXEGE3W42HHT72B0 It has worked so far for our histology applications and our microscopes waterbath, but I would use 'good' purchased water for any molecular biology applications. Sigma has some. You can also get a good deal on 5gallon jugs from mcmastercarr ( http://www.mcmaster.com/#distilled-water/=11fsnq8) of both distilled and deionized water (about $25 each) - but they cost a lot of money (and pollution) to deliver. That being said I do not know the carbon footprint of my distillation machine. Oh, how I miss my millipore system, but OH MY they cost a lot of money to buy, install and fix when they go wrong. Too much for this little start up. Our usage does not afford that luxury! yours, mills On Mon, Mar 7, 2016 at 9:50 AM, Charles Riley via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Does anyone know of a cheap way to make your own DI water? Or a good > reliable company in the Delaware area that provides DI water services? > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > > Doctors Pathology Services, Dover DE > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From classicdoc at gmail.com Mon Mar 7 13:32:41 2016 From: classicdoc at gmail.com (Douglas Gregg) Date: Mon, 7 Mar 2016 14:32:41 -0500 Subject: [Histonet] Histonet Digest, Vol 148, Issue 6 In-Reply-To: References: Message-ID: Message: 5 Date: Mon, 7 Mar 2016 12:50:51 -0500 From: Charles Riley To: histonet at lists.utsouthwestern.edu Subject: [Histonet] DI water Message-ID: Content-Type: text/plain; charset=UTF-8 Does anyone know of a cheap way to make your own DI water? Or a good reliable company in the Delaware area that provides DI water services? -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE You might want to check your local bottled water company. Many are now providing 5 gallon jugs of steam distilled water. That is better than DI water and they deliver. Doug Gregg DVM, PhD Southold, NY On Mon, Mar 7, 2016 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Florida Society for Histotechnology Spring Meeting (John Shelley) > 2. Blog post--lab related (Lester Raff MD) > 3. Whale skin (Kathleen Jones) > 4. Re: Whale skin (Rene J Buesa) > 5. DI water (Charles Riley) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 7 Mar 2016 14:38:19 +0000 > From: John Shelley > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Florida Society for Histotechnology Spring Meeting > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Hi All, > > Happy Monday! > > I would like to inform all histonetters both in Florida and abroad that > our meeting program has been finalized and registration is now open. We > have a wonderful array of workshops that will appeal to all levels of > experience. We have our 3rd annual All-Day Supervisor and Administrator > Forum on Friday, May 20th, 2016 along with our All-Day HT Preparedness > Workshop for students and those who are looking for a fresher on Saturday, > May 21st, 2016. Along with general histology, safety, IHC, molecular, > digital imaging and much more. Please use links below to look at our > program and then our links for registration to the meeting and hotel. > > FSH Online Registration > > FSH Online Program< > http://www.fshgroup.org/wp-content/uploads/2016/03/FSH-2016-Online-ProgramJS1.pdf > > > > FSH Hotel Registration< > http://www.hilton.com/en/hi/groups/personalized/S/SPTSHHF-FSH-20160519/index.jhtml?WT.mc_id=POG > > > > Look forward to seeing you all in sunny and warm St. Pete., Florida at the > Bayfront Hilton on May 19-22, 2016. > > Kind Regards! > > John J Shelley > 2014-2016 FSH President > > Histology Core Manager > Sanford Burnham Prebys Medical Discovery Institute at Lake Nona > > > > ------------------------------ > > Message: 2 > Date: Mon, 7 Mar 2016 15:21:01 +0000 > From: Lester Raff MD > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: [Histonet] Blog post--lab related > Message-ID: > > <6347C6D2B080534F9B5C2B08436DCFAF0B72E334 at COLOEXCH01.uropartners.local> > > Content-Type: text/plain; charset="us-ascii" > > http://www.chicagonow.com/downsize-maybe/2016/03/no-lovie-for-our-lab/ > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > > > ------------------------------ > > Message: 3 > Date: Mon, 07 Mar 2016 12:16:53 -0400 > From: "Kathleen Jones" > To: > Subject: [Histonet] Whale skin > Message-ID: <56DD71350200008B0006DE01 at oes-grpwise.novell.upei.ca> > Content-Type: text/plain; charset=US-ASCII > > Hello, > > I have to cut FFPE blocks of whale skin for in IHC study. I am having > difficulty with both cutting and floating. Any advice would be much > appreciated! > > Thank you! > > > > > > Kathleen Jones > Research Technologist > Pathology/Microbiology > AVC - UPEI > > > > > ------------------------------ > > Message: 4 > Date: Mon, 7 Mar 2016 16:57:23 +0000 (UTC) > From: Rene J Buesa > To: Kathleen Jones , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Whale skin > Message-ID: > <914914500.3949222.1457369843481.JavaMail.yahoo at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > How did you manage to deal with the about 0.5 m of blubber?Was it the skin > of a new born whale? I just to not understand, but you have to completely > eliminate all the fat and increase your processing protocol (infiltration > specially) to have some chance of getting any relatively "decent" > section.Ren? > > On Monday, March 7, 2016 11:23 AM, Kathleen Jones via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hello, > > I have to cut FFPE blocks of whale skin for in IHC study. I am having > difficulty with both cutting and floating. Any advice would be much > appreciated! > > Thank you! > > > > > > Kathleen Jones > Research Technologist > Pathology/Microbiology > AVC - UPEI > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 5 > Date: Mon, 7 Mar 2016 12:50:51 -0500 > From: Charles Riley > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] DI water > Message-ID: > r9NsWWHwircYn3RqPz9mCN6E8ZwF7KxKrayBw at mail.gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Does anyone know of a cheap way to make your own DI water? Or a good > reliable company in the Delaware area that provides DI water services? > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > > Doctors Pathology Services, Dover DE > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 148, Issue 6 > **************************************** > From gmartin at marshallmedical.org Mon Mar 7 14:12:13 2016 From: gmartin at marshallmedical.org (Martin, Gary) Date: Mon, 7 Mar 2016 12:12:13 -0800 Subject: [Histonet] Knife holder Message-ID: <6ED9D4252F278841A0593D3D788AF24C1CFE97B5@mailsvr.MARSHMED.local> I'm looking for a high profile knife holder for my Micron HM 325 microtome, does anyone know where I might get hold of this part. Thanks Gary From Kathy.Machado at LPNT.net Tue Mar 8 08:33:08 2016 From: Kathy.Machado at LPNT.net (Kathy.Machado at LPNT.net) Date: Tue, 8 Mar 2016 14:33:08 +0000 Subject: [Histonet] fluids for Cytology Message-ID: We have a cytology assistant that does all cyto prep and also sends out the pap smears since we no longer have a cyto tech. Kathy Machado, HTL Danville Regional Medical Center Danville, VA Kathy.Machado at lpnt.net 434-799-3867 From TNMayer at mdanderson.org Tue Mar 8 08:40:07 2016 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Tue, 8 Mar 2016 14:40:07 +0000 Subject: [Histonet] Histonet Digest, Vol 148, Issue 6 In-Reply-To: References: Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC882814AA17@D1PWPEXMBX05.mdanderson.edu> Kathleen, I would suggest you contact one of the Veterinary Schools for assistance. I remember that we had some when I was at LSU Vet School, but not much else. Also try one of the Marine Biology programs down in Florida, they may be able to help. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 The information contained in the e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. Message: 3 Date: Mon, 07 Mar 2016 12:16:53 -0400 From: "Kathleen Jones" To: Subject: [Histonet] Whale skin Message-ID: <56DD71350200008B0006DE01 at oes-grpwise.novell.upei.ca> Content-Type: text/plain; charset=US-ASCII Hello, I have to cut FFPE blocks of whale skin for in IHC study. I am having difficulty with both cutting and floating. Any advice would be much appreciated! Thank you! Kathleen Jones Research Technologist Pathology/Microbiology AVC - UPEI ------------------------------ Message: 4 Date: Mon, 7 Mar 2016 16:57:23 +0000 (UTC) From: Rene J Buesa To: Kathleen Jones , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Whale skin Message-ID: <914914500.3949222.1457369843481.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 How did you manage to deal with the about 0.5 m of blubber?Was it the skin of a new born whale? I just to not understand, but you have to completely eliminate all the fat and increase your processing protocol (infiltration specially) to have some chance of getting any relatively "decent" section.Ren? On Monday, March 7, 2016 11:23 AM, Kathleen Jones via Histonet wrote: Hello, I have to cut FFPE blocks of whale skin for in IHC study. I am having difficulty with both cutting and floating. Any advice would be much appreciated! Thank you! Kathleen Jones Research Technologist Pathology/Microbiology AVC - UPEI ********************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From akbitting at geisinger.edu Tue Mar 8 11:25:14 2016 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Tue, 8 Mar 2016 17:25:14 +0000 Subject: [Histonet] Auramine Rhodamine staining Message-ID: <5aacf6c490844902990470eab47914ee@LOFEXMBX108W12V.geisinger.edu> Hi Histonet Colleagues, I'm surveying Histology labs about Auramine-Rhodamine staining for acid fast organisms. If you'd like to help me by participating in my survey please follow the link below. Thank you in advance for your responses. https://www.surveymonkey.com/r/7JPL5K2 Angie Angela K. Bitting, HT(ASCP),QIHC Analytical Specialist, Histology Geisinger Health System 100 N. Academy Avenue Danville, PA 17822-1920 t. 570-214-9634 f. 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From lhamilton at wcplaboratories.com Tue Mar 8 14:45:02 2016 From: lhamilton at wcplaboratories.com (Lisa Hamilton) Date: Tue, 8 Mar 2016 14:45:02 -0600 Subject: [Histonet] Lead Tech Position Message-ID: <000301d1797b$64ca8fb0$2e5faf10$@com> Our lab is currently seeking a highly motivated lead histotech for a full-time night position. We are located just outside St. Louis Missouri. Please contact me if interested. Lisa Hamilton, HT (ASCP) Histology, PA Manager (314) 991-4363 x239 From jvickroy at SpringfieldClinic.com Tue Mar 8 16:07:45 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Tue, 8 Mar 2016 22:07:45 +0000 Subject: [Histonet] Billing for skin biopsies Message-ID: <9B1A1501A800064397369BD8072E6BCA06517BA1@E2K10DB.springfieldclinic.com> There was a question today that I felt pretty comfortable answering but still thought I would see what others have found out on this subject. Dermatologists are always asking if there is a lesser pathology charge for a skin lesion removed for cosmetic purposes. My understanding is that while a clinician can charge less for removing something for cosmetic purposes once it goes to the pathology lab the charges are based on diagnosis and therefore the accepted CPT codes are generally 88302 ( plastic repair), 88304 (cyst debridement, skin tag) and 88305 for (other than the cyst, debridement, skin tag, or plastic repair). And.............we can't charge differently just because it was removed for cosmetic purposes. Please let me know your thoughts on this and if things are done differently at your institution. I have told a clinician that they might not have to submit the "cosmetic" skin biopsy for pathology however I also don't believe that is a good idea either. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From Megan.Dishop at childrensmn.org Wed Mar 9 12:06:23 2016 From: Megan.Dishop at childrensmn.org (Megan Dishop) Date: Wed, 9 Mar 2016 12:06:23 -0600 Subject: [Histonet] Histology lead position in the Twin Cities Message-ID: <56E011BF.D202.00E3.1@childrensmn.org> Dear Histonet, Are you needing a change? We are looking for an experienced Histotechnologist to serve in a leadership role at Children's Minnesota laboratories. Minneapolis-St. Paul is a wonderful place to live, and we are a growing laboratory for a top-notch private, non-profit 420-bed pediatric healthcare system in the Twin Cities area. We are seeking someone with interest and expertise in immunohistochemistry, quality, and operational efficiency. HTL is preferred, but not required. Please take a look at the job listing via the links below and apply online. https://www.childrensmn.org/working-at-childrens/ http://childrensmn.taleo.net/careersection/ex/jobdetail.ftl?job=1602705I We hope you will find the right fit as a member of our team! Best, Megan Dishop Medical Director of Anatomic Pathology Children's Hospitals and Clinics of Minnesota Megan K. Dishop MD Medical Director, Pediatric Anatomic Pathology Children's Hospitals and Clinics of Minnesota Laboratories 2525 Chicago Ave S. MS32-B600, Minneapolis, MN 55404 USA Phone: 612-813-6521 Fax: 612-813-7721 Email: megan.dishop at childrensMN.org Adjoint Professor of Pediatrics, University of Colorado School of Medicine Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. From tbraud at holyredeemer.com Wed Mar 9 12:40:25 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 9 Mar 2016 18:40:25 +0000 Subject: [Histonet] Skin billing Message-ID: <48E053DDF6CE074DB6A7414BA05403F8065024@HRHEX02-HOS.holyredeemer.local> Our institution follows the CPT coding and associated billing, just as you explained. For those surgeries done within our facilities, there is a specific list of specimens which must be submitted. This was agreed upon by med staff and is listed in the bylaws, in response to the following CAP question: ANP.10032 Surgical Pathology Microscopic Exemptions Phase I There is a policy regarding what types of surgical specimens (if any) may be exempt from microscopic examination. NOTE: Irrespective of any exemptions, microscopic examination should be performed whenever there is a request by the submitting or attending physician, or at the discretion of the pathologist when indicated by the clinical history or gross findings. If there is such a policy, it should be approved by the medical staff or appropriate committee. Typical exempt specimens include foreskins in children, prosthetic cardiac valves without attached tissue, torn meniscus, varicose veins, tonsils in children below a certain age, etc. As far as physician's offices and the rules that regulate them, I don't know. Generally, the 88302 and 88304 are lesser charges anyway. I hope this helps. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 ------------------------------ Message: 2 Date: Tue, 8 Mar 2016 22:07:45 +0000 From: "Vickroy, James" Subject: [Histonet] Billing for skin biopsies There was a question today that I felt pretty comfortable answering but still thought I would see what others have found out on this subject. Dermatologists are always asking if there is a lesser pathology charge for a skin lesion removed for cosmetic purposes. My understanding is that while a clinician can charge less for removing something for cosmetic purposes once it goes to the pathology lab the charges are based on diagnosis and therefore the accepted CPT codes are generally 88302 ( plastic repair), 88304 (cyst debridement, skin tag) and 88305 for (other than the cyst, debridement, skin tag, or plastic repair). And.............we can't charge differently just because it was removed for cosmetic purposes. Please let me know your thoughts on this and if things are done differently at your institution. I have told a clinician that they might not have to submit the "cosmetic" skin biopsy for pathology however I also don't believe that is a good idea either. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com ************************************** From mjdessoye at commonwealthhealth.net Wed Mar 9 13:40:56 2016 From: mjdessoye at commonwealthhealth.net (Dessoye, Michael) Date: Wed, 9 Mar 2016 19:40:56 +0000 Subject: [Histonet] Peloris baskets Message-ID: Hello Histonet, Does anyone know of any baskets (preferably spaced) that are compatible with the Leica Peloris? I'm looking for alternatives to the astronomically-priced factory baskets. Any thoughts? Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From BZIMMERM at gru.edu Wed Mar 9 15:18:39 2016 From: BZIMMERM at gru.edu (Zimmerman, Billie) Date: Wed, 9 Mar 2016 21:18:39 +0000 Subject: [Histonet] Histopalooza III Georgia Society for Histotechnology Message-ID: I'm happy to announce that Joe Meyers will be one of the presenters at Histopalooza. On Friday April 22nd it will be the Interpretation of Immuno Stained Slides. His second presentation will be on Sunday morning, April 24th, titled "New Laboratory-Developed Testing Regulations. I read a paragraph about it and decided I have to attend the workshop to find out what it's all about. Seems like more regulations by the government. Joe is an energetic speaker so I'm sure we won't be dozing. Joe, if you're reading this don't you dare cram a bunch of stuff on the PowerPoint with a font size of 2.5, or I will heckle you personally. I need the AARP font size, please. See you next month. Billie Zimmerman MT(ASCP)QIHC GSH secretary (Gigi to Skylar, Landon, Emily , and Grant) From criley at dpspa.com Thu Mar 10 06:42:40 2016 From: criley at dpspa.com (Charles Riley) Date: Thu, 10 Mar 2016 07:42:40 -0500 Subject: [Histonet] Happy National Histotechnology Professionals day Message-ID: Hello everyone just wanted to wish all of my fellow Histotechnology professionals a Happy Histo day. Keep up all the great work you all do. You are all greatly appreciated and are an extremely important part of patient healthcare -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE From relia1 at earthlink.net Thu Mar 10 08:00:25 2016 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 10 Mar 2016 09:00:25 -0500 Subject: [Histonet] Happy Histotechnology Professionals Day from Pam Barker and RELIA Solutions. Message-ID: <008001d17ad5$325b4500$9711cf00$@earthlink.net> Hi Histopeeps, Happy Histotechnology Professionals Day! Kudos to you. For everything you do. Saving Lives Every Day! Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From LRaff at uropartners.com Thu Mar 10 09:56:21 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 10 Mar 2016 15:56:21 +0000 Subject: [Histonet] Also saluting you all today! Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B73A71F@COLOEXCH01.uropartners.local> I join in saluting all the histology professionals out there. We are holding off on our in-lab celebration until next week when our outstanding supervisor will be back to join us. By the way, we still have an opening in our lab. Besides first shift, 2nd or 3rd shift applicants will be considered. Latest (non-professional related) post http://www.chicagonow.com/downsize-maybe/2016/03/construction-progress/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From melissa at alliedsearchpartners.com Thu Mar 10 10:24:17 2016 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Thu, 10 Mar 2016 16:24:17 +0000 Subject: [Histonet] Histology Jobs in New York & Happy Histology Professionals Day! Message-ID: Hi Histoland, Firstly, Happy Histology Professionals day to everyone in this wonderful field! Just a quick note to let you know I am looking for qualified candidates for the following positions in the Port Chester, NY Area: IHC Manager IHC Technologist Histotech Message me for details! Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 From Timothy.Morken at ucsf.edu Thu Mar 10 10:54:32 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 10 Mar 2016 16:54:32 +0000 Subject: [Histonet] Leica Bond and IF staining Message-ID: <761E2B5697F795489C8710BCC72141FF6FD2782E@ex07.net.ucsf.edu> We've been adapting the Leica Bond to do immunofluorescence staining and finding it a hard road. Leica does not support IF staining within the standard clinical software so we need to buy a FISH kit (just one vial of formamide) in order to get a "kit" barcode to run the system. We dump the formamide and put in our own buffer. Totally useless to us, but they say it is the only way to run the system for IF. For that they charge us a price that significantly increases our reagent costs. So, has anyone figured out if it is possible to spoof the system to avoid this unnecessary expense and hassle? Contact me privately you wish. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From therndon at gmi-inc.com Thu Mar 10 11:45:42 2016 From: therndon at gmi-inc.com (Travis Herndon) Date: Thu, 10 Mar 2016 17:45:42 +0000 Subject: [Histonet] Happy Histo Day Message-ID: <94E4C52BB571D647BEA65EBB03750E02071D8B0D@GMI-2011.GMI.local> Happy Histotechnology day. Thank you for all that you do. Kind regards, Travis Herndon Technical Sales Consultant GMI therndon at gmi-inc.com | www.gmi-inc.com Advancing Science with Affordable Solutions ISO 9001:2008 Certified T: 763 712-8717 ext. 6826 | F: 763 712-8724 Cell:612-803-1820 www.linkedin.com/in/travisherndon From jill.cox at cox.net Thu Mar 10 11:46:29 2016 From: jill.cox at cox.net (Jill Cox) Date: Thu, 10 Mar 2016 10:46:29 -0700 Subject: [Histonet] Slide printer input Message-ID: <1078625B-BFC9-44BC-8B49-E7C90228EF6C@cox.net> Hi all! We are switching pathology modules and looking into slide printers, we were told we can use a zebra or primera, also any printer that will print a 1" x 1" label. Any recommendations out there? I have used Dymo in the past. New system is Modmed. Thank you all, and happy Histotechnology Professionals day!! Sent from my iPhone From sbonner at pathregional.com Thu Mar 10 12:50:55 2016 From: sbonner at pathregional.com (Silvia Bonner) Date: Thu, 10 Mar 2016 18:50:55 +0000 Subject: [Histonet] cryostat Message-ID: Hi Everyone, Can you recommend someone for preventative maintenance on cryostats? We have both Shandon and Leica cryostats. Thanks, Silvia Bonner, HT(ASCP) CM Histology Supervisor sbonner at pathregional.com Pathologists' Regional Laboratory 1225 Highland Ave Clarkston, WA 99403 This email may contain physician, patient and/or attorney material that is confidential or privileged for the sole use of the intended recipient. YOU ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED. If you are not the intended recipient, please notify us immediately by telephone at (208) 746-0516 or (509) 758-5576 and delete all copies. From blayjorge at gmail.com Thu Mar 10 12:53:01 2016 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Thu, 10 Mar 2016 13:53:01 -0500 Subject: [Histonet] Improving fixation of insect parts for TEM Message-ID: Hello Histonet-Listers: This is a follow up on earlier messages re. fixation of insect parts for TEM. First, thanks for the feedback to try minimizing the effect of setae in creating an air bubble. Second, we are having difficulties making the resin for blocks used in TEM adhere well to the insect exoskeleton.It seems that there is something on the exoskeleton preventing this. Any constructive suggestions (e.g. washing the insect with a strong solvent?) to improve adherence will be appreciated. My email is blayjorge at gmail.com Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From Joyce.Fortin at uhsinc.com Thu Mar 10 12:53:04 2016 From: Joyce.Fortin at uhsinc.com (Fortin, Joyce) Date: Thu, 10 Mar 2016 18:53:04 +0000 Subject: [Histonet] Leica ASP6025 Tissue Processor and Cryostats Message-ID: <945e3e5be7af44678966307fab25bea2@CORP-EX13KP103.corp.uhsinc.biz> Could anyone who is using the Leica ASP6025 Tissue Processor please send me their opinions of it-pros and cons? Also, which of the Leica cryostats you like the most? We want the vacuum and UVC disinfection models. Pros and cons... Thank you! UHS of Delaware, Inc. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited, and may be punishable by law. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. From loree.lager at seattlechildrens.org Thu Mar 10 14:52:56 2016 From: loree.lager at seattlechildrens.org (Lager, Loree) Date: Thu, 10 Mar 2016 20:52:56 +0000 Subject: [Histonet] Acetylcholinesterase Message-ID: Hi Histo techs, I hope you're all being appropriately honored on our day! I wondering if anyone is still doing the Acetylcholinesterase for Hirschsprung's in children and has a procedure they're willing to share? We have a pathologist requesting it for a research project, and while we used to do it routinely, before IHC, we are having trouble getting our archived procedure to work. I'm not sure if this is due in part to the absence of a couple of the chemicals (well at least not the same product #) or something else? Thanks in advance for your help! Loree Lager Williams Seattle Children's Hospital Histology CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From bcooper at chla.usc.edu Thu Mar 10 16:43:42 2016 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Thu, 10 Mar 2016 22:43:42 +0000 Subject: [Histonet] Leica ASP6025 Tissue Processor and Cryostats In-Reply-To: <945e3e5be7af44678966307fab25bea2@CORP-EX13KP103.corp.uhsinc.biz> References: <945e3e5be7af44678966307fab25bea2@CORP-EX13KP103.corp.uhsinc.biz> Message-ID: So we actually have a Leica CM1950 in our Histo Lab, as well as a Fisher NX70 Cryostar in our Frozen Section Room in the OR. They are both excellent cryostats (I actually learned how to cryosection on the Leica). When we were in the market for a new Cryostat a year ago, we went with the NX70 because of the ergonomic flexibility and cold disinfection--both really cool (ok, pun truly wasn't intended there) features! Check em both out, and see all the cool bells and whistles. Good luck! Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper at chla.usc.edu -----Original Message----- From: Fortin, Joyce via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, March 10, 2016 10:53 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Leica ASP6025 Tissue Processor and Cryostats Could anyone who is using the Leica ASP6025 Tissue Processor please send me their opinions of it-pros and cons? Also, which of the Leica cryostats you like the most? We want the vacuum and UVC disinfection models. Pros and cons... Thank you! UHS of Delaware, Inc. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited, and may be punishable by law. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From LNormington at uwhealth.org Fri Mar 11 07:35:19 2016 From: LNormington at uwhealth.org (Normington Lacy) Date: Fri, 11 Mar 2016 13:35:19 +0000 Subject: [Histonet] Leica ASP6025 Tissue Processor and Cryostats In-Reply-To: References: <945e3e5be7af44678966307fab25bea2@CORP-EX13KP103.corp.uhsinc.biz> Message-ID: <7F7174244DDD1A49BFBEE8A845BC2F2B1889E0@UWHC-MBX05.uwhis.hosp.wisc.edu> Our laboratory has two Leica CM1950 cryostats in our frozen section area. Pro's: 1) quality and reproducibility of frozen sections have drastically improved 2) ease of use when using Peter's method of embedding 3) UV disinfection, saves many hours 4) easy to use 5) optional vacuum system Con's: 1) specimen discs are not interchangeable with other models and are somewhat expensive 2) the vacuum can sometime clog Overall we are extremely happy with the CM1950s. Lacy Normington, HTL (ASCP)CM Manager, Lab Services Surgical Pathology UW Health , Madison, WI -----Original Message----- From: Cooper, Brian via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, March 10, 2016 4:44 PM To: Fortin, Joyce; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Leica ASP6025 Tissue Processor and Cryostats So we actually have a Leica CM1950 in our Histo Lab, as well as a Fisher NX70 Cryostar in our Frozen Section Room in the OR. They are both excellent cryostats (I actually learned how to cryosection on the Leica). When we were in the market for a new Cryostat a year ago, we went with the NX70 because of the ergonomic flexibility and cold disinfection--both really cool (ok, pun truly wasn't intended there) features! Check em both out, and see all the cool bells and whistles. Good luck! Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper at chla.usc.edu -----Original Message----- From: Fortin, Joyce via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, March 10, 2016 10:53 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Leica ASP6025 Tissue Processor and Cryostats Could anyone who is using the Leica ASP6025 Tissue Processor please send me their opinions of it-pros and cons? Also, which of the Leica cryostats you like the most? We want the vacuum and UVC disinfection models. Pros and cons... Thank you! UHS of Delaware, Inc. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution of this information is prohibited, and may be punishable by law. If this was sent to you in error, please notify the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum at uams.edu Fri Mar 11 07:52:20 2016 From: PAMarcum at uams.edu (Marcum, Pamela A) Date: Fri, 11 Mar 2016 13:52:20 +0000 Subject: [Histonet] PAS Question Message-ID: <6744e515344d4456ad6a44895ab5a539@MAIL13M2N2.ad.uams.edu> We have a problem with the cell membranes separating on our bone marrow slides on our PAS slides done daily. We have tried everything to fix this and stop the problem. We have tried manual staining, bulk staining on the Leica Stainer and the Dako Artisan and all have the same problem. I am at a loss and the only thing left is the temperature of oven we dry slides in set at 65C. We do up 38 bone marrows every night so this is critical for us. If anyone can help please give us advise. Pam ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From relia1 at earthlink.net Fri Mar 11 11:00:15 2016 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 11 Mar 2016 12:00:15 -0500 Subject: [Histonet] RELIA Hot Histology Job Alert 3-11-2016 Get off the bench with this amazing opportunity in Norfolk, VA Clinical Lab Specialist - Pathology Analytics Message-ID: <00f201d17bb7$7c331850$749948f0$@earthlink.net> Hello Histonetters!! TGIF! I have a hot new job I thought I would share with you. The position title is: Clinical Lab Specialist -Pathology Analytics RELIA has been engaged EXCLUSIVELY by a growing lab located in Norfolk, VA in need of a top notch ASCP certified Clinical Lab Specialist with a strong background in analytics and LIS. B. S. Degree, ASCP HT/HTL and 5 years of experience in histology is required. My client offers an excellent salary, benefits, relocation assistance and a 15K SIGN ON BONUS. If this is the right job for you RELIA can make it happen! For more information please contact Pam Barker at relia1 at earthlink.net or toll free at 866-607-3542. RELIA Solutions is the nation's ONLY recruiting firm specializing in the nationwide permanent placement of histology professionals. To sign up for our free histology careers bulletin please send an e-mail to relia1 at earthlink.net and include subscribe in the subject line. Have a fantastic Weekend! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From tbraud at holyredeemer.com Fri Mar 11 12:52:19 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 11 Mar 2016 18:52:19 +0000 Subject: [Histonet] Cryostat with UV disinfection Message-ID: <48E053DDF6CE074DB6A7414BA05403F806586D@HRHEX02-HOS.holyredeemer.local> For those of you that are CAP accredited, and use a cryostat with UV disinfection, how do you get around the CAP requirement listed below? We were told the UV disinfection did not replace wiping down with a tuberculocidal disinfectant at room temp. Do you have an approved tuberculocidal disinfectant that will work at cryostat temps? Curious minds would like to know! ANP.23410 Cryostat Decontamination Phase II There is a written procedure for the decontamination of the cryostat at defined intervals, and under defined circumstances, and decontamination records are evident. NOTE: The cryostat must be defrosted and decontaminated by wiping all exposed surfaces with tuberculocidal disinfectant. The cryostat should be at room temperature during decontamination unless otherwise specified by the manufacturer. This should be done at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. Terri L. Braud, HT(ASCP) From careerstudio at bellsouth.net Fri Mar 11 13:13:51 2016 From: careerstudio at bellsouth.net (Barbara Siegel) Date: Fri, 11 Mar 2016 14:13:51 -0500 Subject: [Histonet] JOB: Histology Technologist for Lab in southern Georgia Message-ID: <000f01d17bca$263f7c00$72be7400$@net> We have a fine pathology services client - full service histo lab using Ventana IHC seeking a Histology Technologist for Shift: 10 pm to 6 am or 11 p.m to 7 am. There is a shift differential. Relocation assistance is available to this desirable geography located 1 hour south of Savannah Georgia This position requires individuals who are able to handle a large volume of work, ability to take direction, & a commitment to consistently create quality solutions. Will performs routine & non-routine activities involved in the preparation of slides, for microscopic evaluation by pathologist(s) Accountabilities: ? Ensure proper accessioning & labeling of all tissue samples. ? Process paperwork associated with accessioning & reporting. ? Ensure proper tissue processing. ? Embed processed tissue in paraffin. ? Perform microtomy of embedded tissue ? Prepare slides for routine Hematoxylin & Eosin staining. ? Perform coverslipping of stained slides either manually or automated ? Prepare solutions & reagents for special stain procedures. ? Perform special stain procedures. ? Perform filing of finished blocks & slides. ? Perform routine maintenance & cleaning of equipment & troubleshoot minor equipment failures. ? Document remedial actions such as repairs & repeated tests. ? Adhere to laboratory?s quality control policies, & document all quality control activities. ? Ensure all corporate safety, quality control & quality assurance standards are met. ? Ensure compliance with all local, federal, CLIA & CAP regulations Requirements: Associates degree & 1+ years of full time exp OR H.S. education or equivalent & 5 years. HT (ASCP) or ASCP eligible OR five years of full time experience Our search has one of the finest biotechnology divisions, focused on the clinical laboratory sector. Please contact David King at biolabcareers at aol.com for more information. Career Studio national search 561-738-6363 ? Visit us on linkedin: http://www.linkedin.com/in/biotechnologyhires From BZIMMERM at gru.edu Fri Mar 11 13:45:13 2016 From: BZIMMERM at gru.edu (Zimmerman, Billie) Date: Fri, 11 Mar 2016 19:45:13 +0000 Subject: [Histonet] Histopalooza III Georgia Society for Histology Message-ID: TGIF !! Today's featured speaker is Vinnie Della Speranza. Vinnie will be presenting a day and a half workshop for those folks seeking HT/HTL certification. The notes say not for the faint of heart. Come well rested and with a supply of your favorite energy drink. My favorite energy drink is Apothic Red but not sure if that would fly with Vinnie. What rhymes with Friday? Wine Seriously, two people from our hospital attended this class last year and both passed the HT test with flying colors recently. It's not the kind of test that you can just wing it with cramming the night before the test. After the intensive boot camp class with Sarge Vinnie, you can relax at the Legacy Lodge. It's a beautiful getaway. See you next month and don't forget your combat boots if you're taking Vinnie's class. Respectfully, Billie Zimmerman MT(ASCP)QIHC From tawnia at ampianstaffing.com Fri Mar 11 14:00:06 2016 From: tawnia at ampianstaffing.com (Tawnia Lindsay) Date: Fri, 11 Mar 2016 20:00:06 +0000 Subject: [Histonet] Traveling Histotechs Message-ID: Hi Histonetters! Spring has sprung and the great jobs a popping up all over the country. I have direct hire and temporary jobs everywhere! I have nice sunny weather jobs, some great outdoor locations and some big city needs. Now is the time to jump into a great new position. Some of the states I am working in right now are: Washington Oregon Nevada Arizona Missouri Pennsylvania With new ones popping up every day! Call me anytime! Tawnia Lindsay Senior Recruiter Ampian Staffing, Inc. 126 W Sego Lily Suite 110 Sandy UT 84070 O:877-229-6996 ext 2009 F:801-253-6127 email: tawnia at ampianstaffing.com Website: ampainstaffing.com From victor_tobias at comcast.net Sat Mar 12 09:04:35 2016 From: victor_tobias at comcast.net (victor_tobias at comcast.net) Date: Sat, 12 Mar 2016 15:04:35 +0000 (UTC) Subject: [Histonet] Slide printer input In-Reply-To: <1078625B-BFC9-44BC-8B49-E7C90228EF6C@cox.net> References: <1078625B-BFC9-44BC-8B49-E7C90228EF6C@cox.net> Message-ID: <1831890176.92032.1457795075121.JavaMail.zimbra@comcast.net> From mgflem at gmail.com Sat Mar 12 13:29:48 2016 From: mgflem at gmail.com (Matthew Fleming) Date: Sat, 12 Mar 2016 13:29:48 -0600 Subject: [Histonet] Part-time histotech needed in Milwaukee, Wisconsin Message-ID: A part-time histotech is needed for an independent dermatopathology laboratory in downtown Milwaukee, WI. The salary is highly competitive and hours are negotiable, though mornings are preferred. If interested, please contact me by email at the address below. Thank you, Matthew Fleming, MD Fleming Dermatopathology Milwaukee, WI mgflem at gmail.com From classicdoc at gmail.com Sun Mar 13 12:32:23 2016 From: classicdoc at gmail.com (Douglas Gregg) Date: Sun, 13 Mar 2016 13:32:23 -0400 Subject: [Histonet] Science fair student help In-Reply-To: References: Message-ID: A big thank you to Heather Marlatt for a "Care" package of practice blocks, accidentally mislabeled plus-slides, cover slips, and aqueous eosin to help my 14 year old student to get started on cutting and staining. Castoffs are always welcome. We are starting today to cut the practice blocks and do some staining. I found a crock pot at the thrift store to use for a warm water bath to float tissue sections. You have to be inventive with a home lab. Joe is joining the NY Histotech Assoc. too. By the time he gets to college, he will have a some good experience and maybe job offers in labs. That is his hope. He will certainly qualify for a work study job. Doug Gregg Veterinary pathologist On Tue, Feb 23, 2016 at 10:59 PM, Douglas Gregg wrote: > Hello, > I have not been on this forum for some time, but still follow it. I am a > retired veterinary pathologist. Last year I posted an idea for a freshman > in high school who wants to do a serious science fair project. He is > already beyond high school science level. His father found me because I > have been a science fair judge for many years at a nearby school district. > At first, I declined, citing that I was retired and did not have a good > project for him. Later I thought about a recent study I read about honey > bees being infected with an Iridovirus. That caught my attention because I > did my dissertation and worked for about 10 years on African swine fever > (ASF) caused by an Iridovirus (now renamed but only moved to a branch > classification by itself). It is the only mammalian Iridovirus disease. > Through mass spectrograph studies, and subtraction analysis of normal bee > data from collapsed colony bees, the US Army found the fingerprint of a > likely Iridovirus infection in colony collapse syndrome. No one has yet > confirmed this by other methods that are more conventional and it has not > been fully accepted due to the new technology used to find it. > > So I suggested to Joe, that he could attempt to identify the virus in bee > larva tissues using histology to find inclusions and immunostaining using > Vector staining system. I used immunostaining through most of my career and > was one of the first to identify a virus infection (ASF) with Vector > staining systems back in the early 80s. > > I have a microtome and a few paraffin mold trays and a few plastic > paraffin tissue holders left over from a consulting project 6 years ago. > What he will need is some paraplast, more plastic molds, a few metal trays, > Harris hematoxylin, eosin, slides, coverslips, permount and later PBS, Tris > and a Vector ABC AP kit which I am very hopeful can be donated by Vector > when we get that far. I know histo labs often have lots of old unused > supplies around that don't necessarily fit into current routines or > machines. If any of you have such supplies that could be used for very > manual processing of tissues, they would be greatly appreciated. A warm > water bath is needed too but we can improvise, if necessary. > > Having reviewed the literature on honeybee colony collapse syndrome and > comparing the pathogenesis with African swine fever, an Iridovirus > infection of bees is a very good fit. I think there is a very high > likelihood that this could be a big breakthrough in the honey bee collapse > problem that thus far has not been answered with the many hypotheses > suggested. This is a worldwide problem that threatens the world food supply > and must be understood and controlled soon. As a onetime bee keeper, it is > close to my heart. I hope some of you can help Joe get into histology. He > is very eager and this could possibly lead to a scholarship or at least a > workstudy position during college in a histolab someday. Due to the > recession, his family can't support this project. He has gotten some small > monetary support from a local bee keeper as well as help acquiring bee > larva from colonies. I am giving him space to set up a lab in my basement > and the necessary solvents, etc and lots of training. This is a very > ambitious project but I think it can be accomplished. He has 2 or 3 years > to get it finished, and hopefully published. Any help would be appreciated. > > Any ideas for crowdsource funding would also be appreciated. > > Douglas Gregg DVM, PhD > Retired - Plum Island Animal Disease Center > Southold, NY 11971 > classicdoc at gmail.com > From karen.pfaff at froedtert.com Mon Mar 14 07:07:47 2016 From: karen.pfaff at froedtert.com (Pfaff, Karen) Date: Mon, 14 Mar 2016 12:07:47 +0000 Subject: [Histonet] Cryostat with UV disinfection In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F806586D@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F806586D@HRHEX02-HOS.holyredeemer.local> Message-ID: Our Mohs Lab is CAP accredited. Every seven days of use, cryostats are shut down. Once machine has been cleaned and dried we use Cavi-wipes(super Sani-Cloth from PDI Inc.) We then document disinfection date on QC Log. We have three cryostats, we rotate a cryostat a week for disinfecting purposes. We added this to our Equipment: Cryostats/Microtomes policy. Hope this helps. Karen Pfaff, HT (ASCP), BA Lead Histotechnician Skin Cancer Center Froedtert Health Phone: 414-805-6010??? Fax: 414-805-5325 -----Original Message----- From: Terri Braud [mailto:tbraud at holyredeemer.com] Sent: Friday, March 11, 2016 12:52 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Cryostat with UV disinfection For those of you that are CAP accredited, and use a cryostat with UV disinfection, how do you get around the CAP requirement listed below? We were told the UV disinfection did not replace wiping down with a tuberculocidal disinfectant at room temp. Do you have an approved tuberculocidal disinfectant that will work at cryostat temps? Curious minds would like to know! ANP.23410 Cryostat Decontamination Phase II There is a written procedure for the decontamination of the cryostat at defined intervals, and under defined circumstances, and decontamination records are evident. NOTE: The cryostat must be defrosted and decontaminated by wiping all exposed surfaces with tuberculocidal disinfectant. The cryostat should be at room temperature during decontamination unless otherwise specified by the manufacturer. This should be done at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. Terri L. Braud, HT(ASCP) Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From murphyv at karmanos.org Mon Mar 14 10:19:17 2016 From: murphyv at karmanos.org (Murphy, Valerie) Date: Mon, 14 Mar 2016 15:19:17 +0000 Subject: [Histonet] Processing human brain xenografts Message-ID: Hello Histonetters, I was wondering if anyone could offer advice for the processing of our human brain xenografts harvested from mice. The tumor specimens are approximately 0.5 x 0.5 x 0.5cm. We have been putting them on our animal program ( 30 min alcohols) but once embedded, the tissue looks brittle and some shrinkage occurs. The morphology looks okay. S hould we put them on our routine program ( 1 hour ethanols and xylenes) or is brain tissue too delicate for that run? Would appreciate any advice. Thank you, Valerie ----------- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. From Karen.Heckford at DignityHealth.org Mon Mar 14 14:02:23 2016 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Mon, 14 Mar 2016 12:02:23 -0700 Subject: [Histonet] Clinical Lab vs. Antomical Pathology Message-ID: Good Morning, I am curious to know how many Pathology Departments get lumped into Clinical Lab? Do you wear the same working attire? Our Pathology Department is really small. We are required to go over the red lines in surgery to get to our Frozen Section Room numerous times a day. They want to put us in the same Scrubs as the Clinical Lab which would not allow us to go over the red line. The color of scrubs designates where you are from in the hospital. I for one do not know how to draw blood or even know what color top tube to use the CLS and phlebotomist do. I only know only about Bone Marrows. I am getting frustrated because to me Anatomical Pathology and Clinical Lab is two different species but they keep trying to treat us the same. The only thing we share is the Lab Director and the Pathologists with Clinical Lab. Feeling like I am not being heard. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From LRaff at uropartners.com Mon Mar 14 14:43:34 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 14 Mar 2016 19:43:34 +0000 Subject: [Histonet] Clinical Lab vs. Antomical Pathology In-Reply-To: References: Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B75B2DA@COLOEXCH01.uropartners.local> In my past career in a medium sized community hospital, both aspects of the lab were melded. But your issue with the frozen sections is a different one. Your director needs to meet with the O.R. or the infection control staff to find a way to allow you into the suite without violating any established protocols. If you are actually entering the O.R., you really should check to see if there is a policy that requires changing scrubs between O.R. area and the lab. Lester Raff, MD Uropartners Laboratory Unrelated (and politally inclined) Blog post: http://www.chicagonow.com/downsize-maybe/2016/03/donald-trump-takes-on-artificial-intelligence/ -----Original Message----- From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, March 14, 2016 2:02 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Clinical Lab vs. Antomical Pathology Good Morning, I am curious to know how many Pathology Departments get lumped into Clinical Lab? Do you wear the same working attire? Our Pathology Department is really small. We are required to go over the red lines in surgery to get to our Frozen Section Room numerous times a day. They want to put us in the same Scrubs as the Clinical Lab which would not allow us to go over the red line. The color of scrubs designates where you are from in the hospital. I for one do not know how to draw blood or even know what color top tube to use the CLS and phlebotomist do. I only know only about Bone Marrows. I am getting frustrated because to me Anatomical Pathology and Clinical Lab is two different species but they keep trying to treat us the same. The only thing we share is the Lab Director and the Pathologists with Clinical Lab. Feeling like I am not being heard. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From eca9 at georgetown.edu Mon Mar 14 15:20:02 2016 From: eca9 at georgetown.edu (Eva Permaul) Date: Mon, 14 Mar 2016 16:20:02 -0400 Subject: [Histonet] Can't get the calculation right today Message-ID: Please, please and please help me. I am fighting a sinus infection and I can not think to save my life. I have to make a buffer solution: 10mM Tris-HCL, 5mM CaCl2, 50% glycerol (v/v), pH7 I have tris base and calcium chloride powder. Can someone please help me by writing it out for me? I am so stuck. Thank you, Eva (the one whose head is about to explode) From patrick.lewis at seattlechildrens.org Tue Mar 15 11:50:03 2016 From: patrick.lewis at seattlechildrens.org (Lewis, Patrick) Date: Tue, 15 Mar 2016 16:50:03 +0000 Subject: [Histonet] Advice on exam prep for the ASCP HTL exam Message-ID: <52ec1c9a51c148c99a6cc3db165a886d@PPWEXM01.childrens.sea.kids> Hi everyone I am going to take my ASCP HTL exam on the 21st of April Any advice for studying? I have been going over the sample online tests for both the HT and the HTL and I bought the Michigan study guide. Thanks Patrick Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From MLashus at pathgroup.com Tue Mar 15 12:42:58 2016 From: MLashus at pathgroup.com (Mighnon Lashus) Date: Tue, 15 Mar 2016 17:42:58 +0000 Subject: [Histonet] Open Histotech Position Message-ID: Hello, We currently have an opening for a 3rd shift Histotech at PathGroup Lab in Chattanooga, TN. The candidate will be doing general histology duties and rotate grossing small specimens. The candidate must be CLIA qualified to gross. Anyone interested please send me an email with your resume. Thanks, Mighnon Lashus, HT (ASCP) Laboratory Manager PathGroup 4071 S. Access Rd, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus at pathgroup.com Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup immediately at 615-562-9255. Thank you From djmr55 at hotmail.com Tue Mar 15 18:12:15 2016 From: djmr55 at hotmail.com (donna mihalik rossi) Date: Tue, 15 Mar 2016 19:12:15 -0400 Subject: [Histonet] Acetylcholinesterase Stain Message-ID: This is in response to a question about the ACE stain for Hirschprung's. Here at Penn State University M.S. Hershey Medical Center we still do the ACE stain using a kit from MBL (Medical and Biological Laboratories CO., LTD.) The instructions come with the kit. It provides enough reagents to do 30 tests. It is relatively easy to do and takes about 1/2 hour from start to finish. The kit is a bit pricey but we used to have a procedure that required us to keep all kinds of little used chemicals around and we felt that this was a safer option. Donna Rossi, HT(ASCP) . Donna J. Rossi From monica.aguilera at irbbarcelona.org Wed Mar 16 03:31:33 2016 From: monica.aguilera at irbbarcelona.org (Monica Aguilera) Date: Wed, 16 Mar 2016 09:31:33 +0100 Subject: [Histonet] Katushka (Far-red fluorescent protein TurboFP63) ex-vivo Message-ID: Hi all, does anyone have a protocol or any recommendation for tissue fixation to preserve the Katushka far red protein expressed under a promoter gene? Many thanks! M?nica -- M?nica Aguilera Pujabet, DVM, PhD Histopathology Facility Institute for Research in Biomedicine - IRB Barcelona Baldiri Reixac, 10 E-08028 Barcelona - Spain Tel: +34 934033776 <%2B34%20934020546> monica.aguilera at irbbarcelona.org From CDavis at che-east.org Wed Mar 16 07:23:23 2016 From: CDavis at che-east.org (Cassie P. Davis) Date: Wed, 16 Mar 2016 12:23:23 +0000 Subject: [Histonet] Clinical Lab vs. Antomical Pathology Message-ID: <5C815EADE724D14AA0CC8F037C4185F03335AA1B@SB01MSTMBX13.sb.trinity-health.org> Karen, I agree with Lester, you need support. Unfortunately, histology often gets "lumped in" with clinical laboratory medicine (in my experience) and our leadership is often a med tech. I have nothing but sincere respect for medical techs, anatomical pathology is just a different animal. Where is your medical director in this? Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From MICHELE.FRENCH at bms.com Wed Mar 16 10:32:24 2016 From: MICHELE.FRENCH at bms.com (FRENCH, MICHELE) Date: Wed, 16 Mar 2016 15:32:24 +0000 Subject: [Histonet] Contact Lab for Image Analysis Message-ID: <9552add0ec2c43a193f2b3839202a243@CO2PR26MB0027.067d.mgd.msft.net> Has anyone used a contract organization (other than Flagship) that can do image analysis of IHC slides from clinical specimens? ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From LRaff at uropartners.com Wed Mar 16 11:31:46 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 16 Mar 2016 16:31:46 +0000 Subject: [Histonet] Lab related blog post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B7606C2@COLOEXCH01.uropartners.local> For those of you who do FISH... http://www.chicagonow.com/downsize-maybe/2016/03/fish-a-little-known-lab-test-for-an-important-disease/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From agleiberman at buffalobiolabs.com Wed Mar 16 13:08:25 2016 From: agleiberman at buffalobiolabs.com (Anatoli Gleiberman) Date: Wed, 16 Mar 2016 18:08:25 +0000 Subject: [Histonet] Katushka (Far-red fluorescent protein TurboFP63) ex-vivo In-Reply-To: References: Message-ID: Hi Monica, The majority of fluorescent proteins (eGFP, RFP, Cherry, Tomato) are well preserved after 4-6h fixation with 4% formaldehyde in PBS at +4 C, following PBS washing, sucrose cryo-protection and embedding in OCT. Bright fluorescence is easily seen on cryo-sections. Alternatively, cardiac perfusion by 4% formaldehyde on PBS followed by 1-2h post-fixation with 4% formaldehyde, washing, cryo-protection etc. works even better for the majority of rodent organs (especially for brain). I suspect that Katushka will be preserved as well. Also, there are quite specific antibodies for all these fluorescent proteins including Katushka (anti-tRFP antibody from Evrogen). With these antibodies you can visualize needed protein even on FFPE sections using either HRP-conjugated secondary, or (in case of Katushka) it is possible to try IF using TexasRed conjugated secondary just to keep the signal in the same channel as Katushka itself Anatoli Gleiberman, PhD Director of Histopathology Buffalo Biolabs LLC, 73 High Street Buffalo, NY, 14203 Phone:716-849-6810x354 e-mail:agleiberman at buffalobiolab.com -----Original Message----- From: Monica Aguilera via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 16, 2016 4:32 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Katushka (Far-red fluorescent protein TurboFP63) ex-vivo Hi all, does anyone have a protocol or any recommendation for tissue fixation to preserve the Katushka far red protein expressed under a promoter gene? Many thanks! M?nica -- M?nica Aguilera Pujabet, DVM, PhD Histopathology Facility Institute for Research in Biomedicine - IRB Barcelona Baldiri Reixac, 10 E-08028 Barcelona - Spain Tel: +34 934033776 <%2B34%20934020546> monica.aguilera at irbbarcelona.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbledsoe at iupui.edu Wed Mar 16 16:23:35 2016 From: sbledsoe at iupui.edu (Bledsoe, Sharon B) Date: Wed, 16 Mar 2016 21:23:35 +0000 Subject: [Histonet] old JB4 microtome Message-ID: Does anyone have a knife holder for an old JB4 microtome that they would be willing to part with, for a price of course? I was given an old JB4, I have the base and the specimen holder, but need the glass knife clamp. Thanks, Sharon sbledsoe at iupui.edu This email has been scanned by BullGuard antivirus protection. For more info visit www.bullguard.com From Nancy_Schmitt at pa-ucl.com Thu Mar 17 12:41:19 2016 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Thu, 17 Mar 2016 17:41:19 +0000 Subject: [Histonet] congo red - decolor Message-ID: Happy St. Pat's Day! Any thoughts on how to decolorize Congo Red stains that were done on fat pad slides? We appreciate any insight you might offer:) Nancy United Clinical Labs Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From jaylundgren at gmail.com Thu Mar 17 13:10:14 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 17 Mar 2016 13:10:14 -0500 Subject: [Histonet] congo red - decolor In-Reply-To: References: Message-ID: Are you sure you don't mean Oil Red O stain for fat? Just checking. Congo Red is for amyloid. Destain Congo Red by soaking in 100% Propylene Glycol, warmth and stirring would speed it up. Jay A. Lundgren, M.S., HTL (ASCP) On Thu, Mar 17, 2016 at 12:41 PM, Nancy Schmitt via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Happy St. Pat's Day! > > Any thoughts on how to decolorize Congo Red stains that were done on fat > pad slides? > We appreciate any insight you might offer:) > > Nancy > United Clinical Labs > Dubuque, IA > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jaylundgren at gmail.com Thu Mar 17 16:02:53 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 17 Mar 2016 16:02:53 -0500 Subject: [Histonet] congo red - decolor In-Reply-To: References: Message-ID: Decolorize Congo Red by soaking in a 1% alcoholic solution of Sodium Hydroxide. Heat and stirring might help, be careful not to soak your sections off the slides, NaOH is a strong base. Jay A. Lundgren, M.S., HTL (ASCP) On Thu, Mar 17, 2016 at 1:13 PM, Nancy Schmitt wrote: > Hi Jay- > > > > It really is Congo Red! > > > > Thank you for responding so quickly, > > > > Nancy > > > > > > Nancy Schmitt MLT, HT(ASCP) > > Pathology Support Services Manager > > United Clinical Laboratories > > 205 Bluff Street, Suite 1 > > Dubuque, IA 52001 > > Ph. 563-690-4142 > > Check us out at www.uclaccess.com > > > > > > > > > > *From:* Jay Lundgren [mailto:jaylundgren at gmail.com] > *Sent:* Thursday, March 17, 2016 13:10 PM > *To:* Nancy Schmitt > *Cc:* histonet at lists.utsouthwestern.edu > *Subject:* Re: [Histonet] congo red - decolor > > > > Are you sure you don't mean Oil Red O stain for fat? Just checking. > > > > Congo Red is for amyloid. > > > > Destain Congo Red by soaking in 100% Propylene Glycol, warmth and stirring > would speed it up. > > > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > On Thu, Mar 17, 2016 at 12:41 PM, Nancy Schmitt via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > Happy St. Pat's Day! > > Any thoughts on how to decolorize Congo Red stains that were done on fat > pad slides? > We appreciate any insight you might offer:) > > Nancy > United Clinical Labs > Dubuque, IA > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > NOTICE: This email may contain legally privileged information. The > information is for the use of only the intended recipient(s) even if > addressed incorrectly. If you are not the intended recipient, please notify > the sender that you have received it in error and then delete it along with > any attachments. Thank you. ?? > From ian.bernard at comcast.net Fri Mar 18 02:56:43 2016 From: ian.bernard at comcast.net (ian bernard) Date: Fri, 18 Mar 2016 01:56:43 -0600 Subject: [Histonet] Positive Controls for IHC A/Bodies- Source Message-ID: <006301d180eb$b6ae9660$240bc320$@comcast.net> Hello, I'm looking for a source for acquiring or purchasing good positive controls (slides or blocks) for the following antibodies: - Pan Cytokeratin (AE1/AE3) - Cytokeratin 5/6 (CK 5/6/) - HPV Cocktail Broad Spectrum (1, 6, 11, 16, 18 & 31) - HMW CK + p63 (Basal Cell Cocktail) These are Biocare antibodies. Any thoughts or suggestions? Ian B From LRaff at uropartners.com Fri Mar 18 10:55:30 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 18 Mar 2016 15:55:30 +0000 Subject: [Histonet] Friday fun for some--blog post NOT WORK RELATED Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B761DC6@COLOEXCH01.uropartners.local> Happy weekend to all http://www.chicagonow.com/downsize-maybe/2016/03/march-madness-bracketology-presidential-style/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From denise.long at uconn.edu Fri Mar 18 13:01:44 2016 From: denise.long at uconn.edu (Long, Denise) Date: Fri, 18 Mar 2016 18:01:44 +0000 Subject: [Histonet] Region 1 Histology Meeting Reminder Message-ID: The Region 1 Histology Meeting sponsored by the Connecticut Society for Histotechnologists, Inc. is being held April 15th and 16th at the Mystic Hilton in Mystic, Connecticut. We have three lecture rooms going both days for a total of 24 speakers, plus a full vendor hall. Local attractions include the Submarine Force Museum, the USS Nautilus, Mystic Seaport and Mystic Aquarium and Institute for Exploration is right across the street from the hotel. Gaming and entertain options are available at nearby Foxwoods Resort and Casino, MGM Grand and Mohegan Sun. For more information on the meeting, including a program, contact Denise at denise.long at uconn.edu From aeck at dh.org Fri Mar 18 15:48:58 2016 From: aeck at dh.org (Eck, Allison) Date: Fri, 18 Mar 2016 20:48:58 +0000 Subject: [Histonet] Greening the path lab Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A9721FC1E@DH-MAIL01.dhorg.org> We are currently looking at ways to green our lab and one problem we are running into are the biohazard transport bag. We end up with hundreds everyday and we hate the idea that they end up In the landfill. I'm wondering what others do with these bags. Thanks for any tips Allison From LRaff at uropartners.com Mon Mar 21 08:24:04 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 21 Mar 2016 13:24:04 +0000 Subject: [Histonet] blog Post--always striving to generate public interest in laboratories Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B775A22@COLOEXCH01.uropartners.local> http://www.chicagonow.com/downsize-maybe/2016/03/5-things-your-pathologist-never-told-you/ As always, feel free to forward and spread the word. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From hfedor at jhmi.edu Tue Mar 22 08:45:21 2016 From: hfedor at jhmi.edu (Helen Fedor) Date: Tue, 22 Mar 2016 13:45:21 +0000 Subject: [Histonet] Plastic coverslip Loosening Message-ID: Hello, I did try and search the archives to see if there was any advice on this but didn't find an answer. Do you know of a good way to fix H&E slides that had plastic coverslips where the coverslip is lifting from the slide and in some cases coming completely off? In all cases the tissue is on the coverslip. Thanks in advance. Helen L. Fedor From wbenton at cua.md Tue Mar 22 10:24:48 2016 From: wbenton at cua.md (Walter Benton) Date: Tue, 22 Mar 2016 15:24:48 +0000 Subject: [Histonet] Plastic coverslip Loosening In-Reply-To: References: Message-ID: <6e1b8f9a003544c6a9168d5c0214ec91@MAIL01.GCU-MD.local> This may work. I have used a version of this product on glass coverslips and slides to transfer paraffin sections. http://www.newcomersupply.com/product/mount-quick Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: Helen Fedor via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, March 22, 2016 9:45 AM To: HISTONET (histonet at lists.utsouthwestern.edu) Subject: [Histonet] Plastic coverslip Loosening Hello, I did try and search the archives to see if there was any advice on this but didn't find an answer. Do you know of a good way to fix H&E slides that had plastic coverslips where the coverslip is lifting from the slide and in some cases coming completely off? In all cases the tissue is on the coverslip. Thanks in advance. Helen L. Fedor _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From criley at dpspa.com Tue Mar 22 10:26:49 2016 From: criley at dpspa.com (Charles Riley) Date: Tue, 22 Mar 2016 11:26:49 -0400 Subject: [Histonet] Processing issues Message-ID: We are having issues with our tissue processing. We use the thermo shandon excelsior processor. All steps have agitation and under vacuum. Our routine process is as follows 1. Formalin 1hr 2. Formalin 1hr 3. 75% alcohol 30 mins 4.85% alcohol 30 mins 5. 95% alcohol 30 mins 6.95% alcohol 30 mins 7. 100% alcohol 30 mins 8. 100% alcohol 30 mins 9 xylene 30 mins 10. xylene 30 mins 11 xylene 30 mins 12. Wax for 30 mins 13. Wax 30 mins 14. Wax 30 minutes. Can anyone give any suggestions for altering this process to work better? I know the process should probably be longer however medical director does not want to delay turn around time. If possible keep the process to under 9 hours. If this isn't conducive to consistent processing please explain why so I can show my superiors to give myself some leverage to change things -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE From Timothy.Morken at ucsf.edu Tue Mar 22 10:40:51 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 22 Mar 2016 15:40:51 +0000 Subject: [Histonet] Plastic coverslip Loosening In-Reply-To: <6e1b8f9a003544c6a9168d5c0214ec91@MAIL01.GCU-MD.local> References: <6e1b8f9a003544c6a9168d5c0214ec91@MAIL01.GCU-MD.local> Message-ID: <761E2B5697F795489C8710BCC72141FF6FD2AA32@ex07.net.ucsf.edu> A warning on the Mount-Quick... We have used this for years for our histochemistry slides but we stopped using Quickmount recently when we got a batch that failed - it seemed a bit more viscous than usual while coverslipping and then a couple months later all the coverslips were found to be loosening as the media dried up and peeled off the slide, along with the coverslip. We have had to re-coverslip hundreds of slides. Our last order to Newcomer for this product was never filled so I'm wondering if they got other reports of a bad batch. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Walter Benton via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, March 22, 2016 8:25 AM To: Helen Fedor; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Plastic coverslip Loosening This may work. I have used a version of this product on glass coverslips and slides to transfer paraffin sections. http://www.newcomersupply.com/product/mount-quick Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: Helen Fedor via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, March 22, 2016 9:45 AM To: HISTONET (histonet at lists.utsouthwestern.edu) Subject: [Histonet] Plastic coverslip Loosening Hello, I did try and search the archives to see if there was any advice on this but didn't find an answer. Do you know of a good way to fix H&E slides that had plastic coverslips where the coverslip is lifting from the slide and in some cases coming completely off? In all cases the tissue is on the coverslip. Thanks in advance. Helen L. Fedor _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor at jhmi.edu Tue Mar 22 10:42:59 2016 From: hfedor at jhmi.edu (Helen Fedor) Date: Tue, 22 Mar 2016 15:42:59 +0000 Subject: [Histonet] Plastic coverslip Loosening In-Reply-To: References: Message-ID: <8cb927a144b1449eb008fe1a94f89388@ESGMTWEX11.win.ad.jhu.edu> Thank you for your help. This is encouraging. Helen -----Original Message----- From: Glover, Kimberly [mailto:kimberly.glover at polysciences.com] Sent: Tuesday, March 22, 2016 11:38 AM To: Helen Fedor Cc: histonet Subject: RE: Plastic coverslip Loosening Helen, Submerge the slides with coverslip loosely attached in fresh xylene to reactivate the film resin. Allow to sit in xylene for approximately 1 minute. Afterwards remove slide and press down on a paper towel. This action will allow the film to reattach to the slide while pressing out any excess xylene from the slide. As for the film (containing tissue) that has completed detached from the slide, place the film against a clean slide and place in a slide basket. Submerge slide in fresh xylene and proceed with same procedure above. Sometimes the issue of loosening or lifting of film is seen over time when the coverslipped slides are placed in temperature/humidity conditions that are contrary to the manufacturer's recommendations. Check packaging for storage conditions recommended. I hope this helps. Kimberly A. Glover Life Sciences Product Manager Polysciences, Inc. 400 Valley Road Warrington, PA 18976 www.polysciences.com -----Original Message----- From: Helen Fedor [mailto:hfedor at jhmi.edu] Sent: Tuesday, March 22, 2016 11:06 AM To: Glover, Kimberly Subject: RE: Plastic coverslip Loosening The film. -----Original Message----- From: Glover, Kimberly [mailto:kimberly.glover at polysciences.com] Sent: Tuesday, March 22, 2016 10:25 AM To: Helen Fedor Subject: RE: Plastic coverslip Loosening Are you using plastic coverslipping film or the plastic coverslips? Kimberly -----Original Message----- From: Helen Fedor via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, March 22, 2016 9:45 AM To: HISTONET (histonet at lists.utsouthwestern.edu) Subject: [Histonet] Plastic coverslip Loosening Hello, I did try and search the archives to see if there was any advice on this but didn't find an answer. Do you know of a good way to fix H&E slides that had plastic coverslips where the coverslip is lifting from the slide and in some cases coming completely off? In all cases the tissue is on the coverslip. Thanks in advance. Helen L. Fedor _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwerdler at gmail.com Tue Mar 22 11:11:10 2016 From: mwerdler at gmail.com (Mca Werdler) Date: Tue, 22 Mar 2016 10:11:10 -0600 Subject: [Histonet] Processing issues In-Reply-To: References: Message-ID: Hey Charles, Why are the first 2 steps of formalin that long? If you make sure your tissue is well fixated, then there is no need for such long fixation in the machine. You could use step 2 for just washing the formalin out, so for only like 5 minutes. You could use that time, to add to other chemicals. Hope it works for you. Maarten Werdler 2016-03-22 9:26 GMT-06:00 Charles Riley via Histonet < histonet at lists.utsouthwestern.edu>: > We are having issues with our tissue processing. We use the thermo shandon > excelsior processor. All steps have agitation and under vacuum. Our routine > process is as follows > > 1. Formalin 1hr > 2. Formalin 1hr > 3. 75% alcohol 30 mins > 4.85% alcohol 30 mins > 5. 95% alcohol 30 mins > 6.95% alcohol 30 mins > 7. 100% alcohol 30 mins > 8. 100% alcohol 30 mins > 9 xylene 30 mins > 10. xylene 30 mins > 11 xylene 30 mins > 12. Wax for 30 mins > 13. Wax 30 mins > 14. Wax 30 minutes. > > Can anyone give any suggestions for altering this process to work better? I > know the process should probably be longer however medical director does > not want to delay turn around time. If possible keep the process to under 9 > hours. If this isn't conducive to consistent processing please explain why > so I can show my superiors to give myself some leverage to change things > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > > Doctors Pathology Services, Dover DE > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mwerdler at gmail.com Tue Mar 22 11:16:18 2016 From: mwerdler at gmail.com (Mca Werdler) Date: Tue, 22 Mar 2016 10:16:18 -0600 Subject: [Histonet] Vibratome OTS 5000 Message-ID: Good day everyone, Does anyone have expierience with the vibratome OTS 5000? We just got this machine and we have no idea how to work with it. Thank you Maarten Werdler From wdesalvo.cac at outlook.com Tue Mar 22 11:22:47 2016 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Tue, 22 Mar 2016 09:22:47 -0700 Subject: [Histonet] Processing issues Message-ID: Not knowing what issues you have, I suggest you look to your samples for processing. Reduce thickness and the shorter times will work. 2-2.5 mm thick. Standardize fixation before placing in tissue processor. What time is the processor started and what time does the tech remove? You may be able to reduce delay and increase alcohols. Specimen thickness will reduce the the time for solution to move through the tissue sample and allow exchange of solution. There are many options, but be more specific about the problem and you can narrow the changes Sent from my Windows Phone ________________________________ From: Charles Riley via Histonet Sent: ?3/?22/?2016 8:57 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Processing issues We are having issues with our tissue processing. We use the thermo shandon excelsior processor. All steps have agitation and under vacuum. Our routine process is as follows 1. Formalin 1hr 2. Formalin 1hr 3. 75% alcohol 30 mins 4.85% alcohol 30 mins 5. 95% alcohol 30 mins 6.95% alcohol 30 mins 7. 100% alcohol 30 mins 8. 100% alcohol 30 mins 9 xylene 30 mins 10. xylene 30 mins 11 xylene 30 mins 12. Wax for 30 mins 13. Wax 30 mins 14. Wax 30 minutes. Can anyone give any suggestions for altering this process to work better? I know the process should probably be longer however medical director does not want to delay turn around time. If possible keep the process to under 9 hours. If this isn't conducive to consistent processing please explain why so I can show my superiors to give myself some leverage to change things -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Tue Mar 22 13:49:43 2016 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 22 Mar 2016 14:49:43 -0400 Subject: [Histonet] Tri-State Histology Symposium (Minnesota, Iowa and Wisconsin) in Cedar Rapids, IA, April 27-29. Message-ID: <011001d1846b$99ed06f0$cdc714d0$@earthlink.net> Hi Histonetters! I hope you are all having a great day. I am posting this information for a friend. You are invited to participate in the ?Tri-State Histology Symposium (Minnesota, Iowa and Wisconsin) in Cedar Rapids, IA, April 27-29.? We have combined three states together to get the most Vendors and Attendees and to be able to offer the best speakers. For more information please see the WHS website www.whs.wildapricot.org Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From SteveM at mcclainlab.com Wed Mar 23 11:50:09 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Wed, 23 Mar 2016 16:50:09 +0000 Subject: [Histonet] Histonet Digest, Vol 148, Issue 20 processing In-Reply-To: References: Message-ID: I don't really like wasting valuable rapid-processing time on fixation, when I cab icontrol that at the bench Your schedule is made more difficult because of the length of time in formalin. See if your director can ensure only fixed tissue on processor. If not increase temp to 45C station #1 and change frequently allowing only 1 hour in warm formalin. This will gain an 45 min for 100% and paraffin (two stages where you are disproportionately short). Then remove the formalin station #2 replace w 50% alc 15 minutes rinses buffer salts off the seals lengthening life of processor seals. 3 75% alc 30 4 95% alc decrease to 15 min 5 95% alc 45 min 6 100% alc increase to 15 min 7 100% alc decrease to 30 min 8 100 % alc increase to 45 9 xylene 1 decrease to 15 10 xylene 2 same 30 11 zylene 3 increase to 45 12 paraffin 1 decrease to 15 13 paraffin 2 same 30 14 paraffin 3 increase to 90 min Should give you just under 9 hours. Since you are constrained on time and depend on consistent, rapid diffusion, rotate solutions frequently. Steve A. McClain, MD > On Mar 22, 2016, at 13:53, "histonet-request at lists.utsouthwestern.edu" wrote: > > 1. Formalin 1hr > 2. Formalin 1hr > 3. 75% alcohol 30 mins > 4.85% alcohol 30 mins > 5. 95% alcohol 30 mins > 6.95% alcohol 30 mins > 7. 100% alcohol 30 mins > 8. 100% alcohol 30 mins > 9 xylene 30 mins > 10. xylene 30 mins > 11 xylene 30 mins > 12. Wax for 30 mins > 13. Wax 30 mins > 14. Wax 30 minutes. From nlinke at sbch.org Wed Mar 23 18:27:31 2016 From: nlinke at sbch.org (Noelle Linke) Date: Wed, 23 Mar 2016 23:27:31 +0000 Subject: [Histonet] bone marrow clots Message-ID: Hi all, Question: Does anyone produce beautiful, well fixed bone marrow clot blocks that has a procedure they would be willing to share? Do you all use the specimen from the EDTA tube after prepping the smears? Any help would be greatly appreciated! Thank you, No?lle No?lle Linke, MS, HTL(ASCP) QIHC Manager, Anatomic Pathology Pacific Diagnostic Laboratories nlinke at sbch.org Phone: (805) 324-9814 Fax: (805) 696-6433 ________________________________ CH Disclaimer: This electronic mail message is intended exclusively for the individual or entity to which it is addressed. This message, together with any attachment, may contain confidential and privileged information. Any views, opinions or conclusions expressed in this message are those of the individual sender and do not necessarily reflect the views of Cottage Health, its subsidiaries or affiliates. This document may also contain information covered under the Health Insurance Portability and Accountability Act (HIPAA, PL 104-191) and implementing regulations and must be protected in accordance with those provisions. Re-disclosure without patient consent or as otherwise permitted by law is prohibited. Any unauthorized review, retransmission, use, printing, copying, retention, disclosure, distribution or the taking of any action in reliance upon this information by persons or entities other than the intended recipient is strictly prohibited and may be unlawful. If you have received this message in error, please immediately advise the sender by reply email message to the sender and delete all copies of this message from your system without copying. ________________________________ From DKBoyd at chs.net Thu Mar 24 06:38:13 2016 From: DKBoyd at chs.net (Boyd, Debbie M) Date: Thu, 24 Mar 2016 11:38:13 +0000 Subject: [Histonet] bone marrow clots In-Reply-To: References: Message-ID: <7EAFE982E328304DA6CE2B677BB76246AB4B2BA3@TN001WEXMBX014.US.chs.net> We use the syringe used for the smears and the syringe used for the aspirate. We do not put it in an anti-coagulate. We simply let the syringe specimen clot and place it in lens paper in a cassette and place in 10% formalin for processing. If you are doing Flow Cytometry/Cytogenetic studies you can use 1ml for these studies and any left in this syringe can also be used for the aspirate/clot. One of our pathologist will purposely get 2mls in the aspirate syringe. One ml for Flow and Cyto (green top sodium heparinized tube) and the other for the clot. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Noelle Linke via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 23, 2016 7:27 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] bone marrow clots Hi all, Question: Does anyone produce beautiful, well fixed bone marrow clot blocks that has a procedure they would be willing to share? Do you all use the specimen from the EDTA tube after prepping the smears? Any help would be greatly appreciated! Thank you, No?lle No?lle Linke, MS, HTL(ASCP) QIHC Manager, Anatomic Pathology Pacific Diagnostic Laboratories nlinke at sbch.org Phone: (805) 324-9814 Fax: (805) 696-6433 ________________________________ CH Disclaimer: This electronic mail message is intended exclusively for the individual or entity to which it is addressed. This message, together with any attachment, may contain confidential and privileged information. Any views, opinions or conclusions expressed in this message are those of the individual sender and do not necessarily reflect the views of Cottage Health, its subsidiaries or affiliates. This document may also contain information covered under the Health Insurance Portability and Accountability Act (HIPAA, PL 104-191) and implementing regulations and must be protected in accordance with those provisions. Re-disclosure without patient consent or as otherwise permitted by law is prohibited. Any unauthorized review, retransmission, use, printing, copying, retention, disclosure, distribution or the taking of any action in reliance upon this information by persons or entities other than the intended recipient is strictly prohibited and may be unlawful. If you have received this message in error, please immediately advise the sender by reply email message to the sender and delete all copies of this message from your system without copying. ________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From LRaff at uropartners.com Thu Mar 24 08:32:27 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 24 Mar 2016 13:32:27 +0000 Subject: [Histonet] How does your lab handle lunch meetings? (blog post) Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B788732@COLOEXCH01.uropartners.local> http://www.chicagonow.com/downsize-maybe/2016/03/never-let-a-doctor-plan-your-lunch/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From jqb7 at cdc.gov Thu Mar 24 09:08:56 2016 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Thu, 24 Mar 2016 14:08:56 +0000 Subject: [Histonet] cassette colors and scanning Message-ID: <3B2CD438E1628A41BD687E98B963B7815D7CEF4F@EMBX-CLFT4.cdc.gov> Good morning everyone! Do any of you have any advice regarding which color cassettes do not work well with the barcode scanning systems currently in use today? Thanks much! Jeanine H. Sanders Infectious Diseases Pathology Branch Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS-G32 Atlanta, GA 30329 From LNormington at uwhealth.org Thu Mar 24 09:18:45 2016 From: LNormington at uwhealth.org (Normington Lacy) Date: Thu, 24 Mar 2016 14:18:45 +0000 Subject: [Histonet] cassette colors and scanning In-Reply-To: <3B2CD438E1628A41BD687E98B963B7815D7CEF4F@EMBX-CLFT4.cdc.gov> References: <3B2CD438E1628A41BD687E98B963B7815D7CEF4F@EMBX-CLFT4.cdc.gov> Message-ID: <7F7174244DDD1A49BFBEE8A845BC2F2B19C8EF@UWHC-MBX05.uwhis.hosp.wisc.edu> I would not recommend florescent pink, red or aqua. The colors that we currently use are yellow, white, green, lilac, light pink (salmon), and orange (on Printmate's only). We had many trials and tribulations when it came to the barcodes actually scanning. We went through three different scanners until we found the one that was most consistent. The scanner brand we have settled on is Honeywell, model 1900. Good luck. Lacy Normington, HTL(ASCP)CM Manager, Surgical Pathology Lab Services UWHospital and Clinics 600 Highland Avenue Madison, WI 53792-2472 Phone: 608-890-9373 -----Original Message----- From: Sanders, Jeanine (CDC/OID/NCEZID) via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, March 24, 2016 9:09 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] cassette colors and scanning Good morning everyone! Do any of you have any advice regarding which color cassettes do not work well with the barcode scanning systems currently in use today? Thanks much! Jeanine H. Sanders Infectious Diseases Pathology Branch Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS-G32 Atlanta, GA 30329 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From eddessa at emory.edu Thu Mar 24 12:52:18 2016 From: eddessa at emory.edu (Dessasau III, Evan) Date: Thu, 24 Mar 2016 17:52:18 +0000 Subject: [Histonet] Porcessing FFPE tissue without alcohol?? Message-ID: Hi Histonet, is there a way to process tissue for paraffin embedding without using alcohol? One of the labs that send their processing to us will be doing a study examining fat. I told them if they want to look at the fat they will have to cut frozen sections but I'm being ask again about processing without the alcohol. So I said I would ask around. Please let me know what you think. Thank you for your help! E-van ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From rjbuesa at yahoo.com Thu Mar 24 13:32:04 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 24 Mar 2016 18:32:04 +0000 (UTC) Subject: [Histonet] Porcessing FFPE tissue without alcohol?? In-Reply-To: References: Message-ID: <947707747.425951.1458844324888.JavaMail.yahoo@mail.yahoo.com> To embed the tissues with paraffin you HAVE TO dehydrate the tissue. This is usually?done with either ethanol of 2-propanol but essentially all dehydrants will remove fat so you are right, the way to go is going frozen sections.Ren? On Thursday, March 24, 2016 2:24 PM, "Dessasau III, Evan via Histonet" wrote: Hi Histonet,? is there a way to process tissue for paraffin embedding without using alcohol?? ? ? ? ? ? ? ? ? ? ? ? One of the labs that send their processing to us will be doing a study examining? fat.? I told them if they want to look at the fat they will have to cut frozen sections but I'm being ask again about processing without the alcohol.? So I said I would ask around. Please let me know what you think. Thank you for your help! E-van ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mari.Yang at Hoag.org Thu Mar 24 19:10:32 2016 From: Mari.Yang at Hoag.org (Yang, Mari) Date: Thu, 24 Mar 2016 17:10:32 -0700 Subject: [Histonet] Medical Laboratory Survey Invitation Message-ID: <02A9B6BBE70204458C12BF79FEEF018C11050033@HHEXMB03.hoag.org> Dear Histonet, My name is Mari Yang and I am a doctorate student pursuing a degree in Management and Organizational Leadership. I have the unique opportunity to facilitate a study exploring the extent to which spiritual leadership positively influences organizational commitment, productivity, life satisfaction, and negatively influences burnout in the medical laboratory. I invite all persons over the age of 18 who are currently working in a medical laboratory in the US to participate. I am passionate about this study because I believe the findings will add value to the medical laboratory profession. Thank you for taking the time to participate. The public announcement for this study is posted on the ASCP Lab Forum http://bit.ly/224IwVE or you may go directly to the survey https://www.surveymonkey.com/r/77X9X58 Thank you in advance for your contribution. Best regards, Mari Yang, MHA, CT(ASCP)HTL P Save a tree, please don't print this e-mail unless you really need to. Please note that the information contained in this message and any files transmitted with it are privileged and confidential and are protected from disclosure under the law, including the Health Insurance Portability and Accountability Act (HIPAA). If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited and may subject you to criminal or civil penalties. If you have received this communication in error, please notify the sender by replying to the message and delete the material from any computer. Thank you, Hoag Memorial Hospital Presbyterian and its Affiliates From wbenton at cua.md Thu Mar 24 19:45:09 2016 From: wbenton at cua.md (Walter Benton) Date: Fri, 25 Mar 2016 00:45:09 +0000 Subject: [Histonet] Fw: Sliding Microtome In-Reply-To: <2bec04df7b9944fa963e1ca556a90c88@MAIL01.GCU-MD.local> References: <2bec04df7b9944fa963e1ca556a90c88@MAIL01.GCU-MD.local> Message-ID: <1458866716404.87270@cua.md> (2nd attempt)? To all: I have a Neuro-Pathologist colleague that is looking to learn how to use a sliding microtome. Is there anyone out there that would be willing to show him the basics? Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________ From: Walter Benton Sent: Thursday, March 24, 2016 10:53 AM To: histonet at lists.utsouthwestern.edu Subject: Sliding Microtome To all: I have a Neuro-Pathologist colleague that is looking to learn how to use a sliding microtome. Is there anyone out there that would be willing to show him the basics? Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From wbenton at cua.md Fri Mar 25 06:31:41 2016 From: wbenton at cua.md (Walter Benton) Date: Fri, 25 Mar 2016 11:31:41 +0000 Subject: [Histonet] Sliding Microtome In-Reply-To: <1458866716404.87270@cua.md> References: <2bec04df7b9944fa963e1ca556a90c88@MAIL01.GCU-MD.local> <1458866716404.87270@cua.md> Message-ID: <8518db1f56c3432e9335cef9aac2f295@MAIL01.GCU-MD.local> Thanks to those who have responded. Anyone able to share or train on techniques for free floating IHC? He needs that skill set too. -----Original Message----- From: Walter Benton via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, March 24, 2016 8:45 PM To: Histonet Subject: [Histonet] Fw: Sliding Microtome (2nd attempt)? To all: I have a Neuro-Pathologist colleague that is looking to learn how to use a sliding microtome. Is there anyone out there that would be willing to show him the basics? Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________ From: Walter Benton Sent: Thursday, March 24, 2016 10:53 AM To: histonet at lists.utsouthwestern.edu Subject: Sliding Microtome To all: I have a Neuro-Pathologist colleague that is looking to learn how to use a sliding microtome. Is there anyone out there that would be willing to show him the basics? Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From wbenton at cua.md Fri Mar 25 07:56:38 2016 From: wbenton at cua.md (Walter Benton) Date: Fri, 25 Mar 2016 12:56:38 +0000 Subject: [Histonet] Brain IHC Message-ID: <85fed3fb580b436f9522fb408d022020@MAIL01.GCU-MD.local> Please see message below. Feel free to reach out directly to the Pathologist. From: Rudolph J Castellani [mailto:Rudolph.Castellani at med.wmich.edu] Sent: Friday, March 25, 2016 8:55 AM To: Walter Benton Subject: Another question - is there any option of embedding big brain slices in paraffin and doing 50 micron IHC on them, or is that not possible? ________________________________ This electronic message is intended for the named recipient(s) only, and may contain information that is confidential or privileged. If you are not the named recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error, or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. Thank you. CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From BZIMMERM at gru.edu Fri Mar 25 13:03:08 2016 From: BZIMMERM at gru.edu (Zimmerman, Billie) Date: Fri, 25 Mar 2016 18:03:08 +0000 Subject: [Histonet] Histopalooza III Georgia Society for Histotechnology Legacy Lodge Lake Lanier, Georgia Message-ID: The word of the day is procrastination. Procrastination isn't so bad. You'll always have something to do tomorrow. But today is the day you need to reserve your room at the Legacy Lodge! There are 10 rooms remaining at the discounted price. Registration for classes will get you a FREE park entrance. Otherwise, it's $14.50. Hey, I know it's not the Caribbean but you can put your toes in the mud and have a glass of sweet tea. http://www.histosearch.com/gsh/ Happy Easter Billie Zimmerman GSH Secretary From carl.hobbs at kcl.ac.uk Fri Mar 25 13:30:27 2016 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 25 Mar 2016 18:30:27 +0000 Subject: [Histonet] Porcessing FFPE tissue without alcohol?? Message-ID: Fix the tissue in Formalin, wash well in dw, then place very small pieces into Osmium tetroxide solution ( std soln for TEM post-fixation) Processing to Pwax as usual. Basically, you will see lipids as black ( oxidised osmium) That's the only way to demonstrate solvent- soluble lipids, using Pwax processing. Sure, there are caveats but, in the main...it will be Ok, imho. I invite comments as I may be doing exactly this very soon, to count myelinated nerve fibres in a sciatic nerve. Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From rjbuesa at yahoo.com Sat Mar 26 06:32:56 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Sat, 26 Mar 2016 11:32:56 +0000 (UTC) Subject: [Histonet] Porcessing FFPE tissue without alcohol?? In-Reply-To: References: Message-ID: <1076021560.1008554.1458991976574.JavaMail.yahoo@mail.yahoo.com> The only problem I see is that the fat will be preserved, as you wrote, as a black osmium oxidate but you will not be able to use any "standard" fat stain; otherwise it will work.Ren? On Friday, March 25, 2016 2:41 PM, "Hobbs, Carl via Histonet" wrote: Fix the tissue in Formalin, wash well in dw, then place very small pieces into Osmium tetroxide solution ( std soln for TEM post-fixation) Processing to Pwax as usual. Basically, you will see lipids as black ( oxidised osmium) That's the only way to demonstrate solvent- soluble lipids, using Pwax processing. Sure, there are caveats but, in the main...it will be Ok, imho. I invite comments as I may be doing exactly this very soon, to count myelinated nerve fibres in a sciatic nerve. ? Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813? ? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jobaluyut at gmail.com Sat Mar 26 10:20:20 2016 From: jobaluyut at gmail.com (Joanna) Date: Sat, 26 Mar 2016 08:20:20 -0700 Subject: [Histonet] Porcessing FFPE tissue without alcohol?? In-Reply-To: <1076021560.1008554.1458991976574.JavaMail.yahoo@mail.yahoo.com> References: <1076021560.1008554.1458991976574.JavaMail.yahoo@mail.yahoo.com> Message-ID: <893C1E6D-057B-4F37-A483-E897B58D277F@gmail.com> How about Sudan Black stain? > On Mar 26, 2016, at 4:32 AM, Rene J Buesa via Histonet wrote: > > The only problem I see is that the fat will be preserved, as you wrote, as a black osmium oxidate but you will not be able to use any "standard" fat stain; otherwise it will work.Ren? > > On Friday, March 25, 2016 2:41 PM, "Hobbs, Carl via Histonet" wrote: > > > > > Fix the tissue in Formalin, wash well in dw, then place very small pieces into Osmium tetroxide solution ( std soln for TEM post-fixation) > Processing to Pwax as usual. > Basically, you will see lipids as black ( oxidised osmium) > That's the only way to demonstrate solvent- soluble lipids, using Pwax processing. > Sure, there are caveats but, in the main...it will be Ok, imho. > I invite comments as I may be doing exactly this very soon, to count myelinated nerve fibres in a sciatic nerve. > > > > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > 020 7848 6813 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Sat Mar 26 10:35:36 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Sat, 26 Mar 2016 15:35:36 +0000 (UTC) Subject: [Histonet] Porcessing FFPE tissue without alcohol?? In-Reply-To: <893C1E6D-057B-4F37-A483-E897B58D277F@gmail.com> References: <893C1E6D-057B-4F37-A483-E897B58D277F@gmail.com> Message-ID: <933723005.1041563.1459006536249.JavaMail.yahoo@mail.yahoo.com> Sudan Black reacts only with protein-combined fats.Ren? On Saturday, March 26, 2016 11:20 AM, Joanna wrote: How about Sudan Black stain? > On Mar 26, 2016, at 4:32 AM, Rene J Buesa via Histonet wrote: > > The only problem I see is that the fat will be preserved, as you wrote, as a black osmium oxidate but you will not be able to use any "standard" fat stain; otherwise it will work.Ren? > >? ? On Friday, March 25, 2016 2:41 PM, "Hobbs, Carl via Histonet" wrote: > > > > > Fix the tissue in Formalin, wash well in dw, then place very small pieces into Osmium tetroxide solution ( std soln for TEM post-fixation) > Processing to Pwax as usual. > Basically, you will see lipids as black ( oxidised osmium) > That's the only way to demonstrate solvent- soluble lipids, using Pwax processing. > Sure, there are caveats but, in the main...it will be Ok, imho. > I invite comments as I may be doing exactly this very soon, to count myelinated nerve fibres in a sciatic nerve. > > > >? > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge? > Kings College London > London > SE1 1UL >? > 020 7848 6813? ? > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz at premierlab.com Sat Mar 26 10:58:53 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Sat, 26 Mar 2016 09:58:53 -0600 Subject: [Histonet] Porcessing FFPE tissue without alcohol?? In-Reply-To: <893C1E6D-057B-4F37-A483-E897B58D277F@gmail.com> References: <1076021560.1008554.1458991976574.JavaMail.yahoo@mail.yahoo.com>, <893C1E6D-057B-4F37-A483-E897B58D277F@gmail.com> Message-ID: <14E2C6176416974295479C64A11CB9AE02BEAFF7B4BA@SBS2K8.premierlab.local> We use osmium post fixation to look at fat all of the time in mouse liver, nerve and muscle samples. It works well, sample size needs to be thin, samples are friable and can crack easily. We use a specific procedure for this it includes potassium dichromate I think, I'm at home but on Monday I can send the reference. One more thing don't trim into the block too much. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz at premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: Joanna via Histonet [histonet at lists.utsouthwestern.edu] Sent: Saturday, March 26, 2016 9:20 AM To: Rene J Buesa Cc: Hobbs, Carl; histonet Subject: Re: [Histonet] Porcessing FFPE tissue without alcohol?? How about Sudan Black stain? > On Mar 26, 2016, at 4:32 AM, Rene J Buesa via Histonet wrote: > > The only problem I see is that the fat will be preserved, as you wrote, as a black osmium oxidate but you will not be able to use any "standard" fat stain; otherwise it will work.Ren? > > On Friday, March 25, 2016 2:41 PM, "Hobbs, Carl via Histonet" wrote: > > > > > Fix the tissue in Formalin, wash well in dw, then place very small pieces into Osmium tetroxide solution ( std soln for TEM post-fixation) > Processing to Pwax as usual. > Basically, you will see lipids as black ( oxidised osmium) > That's the only way to demonstrate solvent- soluble lipids, using Pwax processing. > Sure, there are caveats but, in the main...it will be Ok, imho. > I invite comments as I may be doing exactly this very soon, to count myelinated nerve fibres in a sciatic nerve. > > > > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > 020 7848 6813 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wgray19 at sc.rr.com Sat Mar 26 11:56:06 2016 From: wgray19 at sc.rr.com (Wanda Shotsberger Gray) Date: Sat, 26 Mar 2016 12:56:06 -0400 Subject: [Histonet] Processing FFPE tissue without alcohol In-Reply-To: References: Message-ID: <153b3daac70.27e6.145c840c2545cc8d226fb8e47258f83d@sc.rr.com> While the tissue will still go through alcohol, have you considered preserving the fat with osmium tetroxide prior to routine processing? This turns the fat black, but it is retained in the tissue. Hi Histonet, is there a way to process tissue for paraffin embedding without using alcohol? One of the labs that send their processing to us will be doing a study examining fat. I told them if they want to look at the fat they will have to cut frozen sections but I'm being ask again about processing without the alcohol. So I said I would ask around. Please let me know what you think. Thank you for your help! E-van Sent with AquaMail for Android http://www.aqua-mail.com From carl.hobbs at kcl.ac.uk Sat Mar 26 13:56:21 2016 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sat, 26 Mar 2016 18:56:21 +0000 Subject: [Histonet] Porcessing FFPE tissue without alcohol?? In-Reply-To: <14E2C6176416974295479C64A11CB9AE02BEAFF7B4BA@SBS2K8.premierlab.local> References: <1076021560.1008554.1458991976574.JavaMail.yahoo@mail.yahoo.com>, <893C1E6D-057B-4F37-A483-E897B58D277F@gmail.com>, <14E2C6176416974295479C64A11CB9AE02BEAFF7B4BA@SBS2K8.premierlab.local> Message-ID: Thanks, Liz. If you look at fat all the time, using Osmium.....you then are not sure if you use K-dichromate? I am a tad confused Also....why not trim the block too much? Best wishes, Carl NB: Rene stated that I wouldn't be able to use any other fat stains...that's the point, Rene. I don't need any other. I commit to Osmium. Yep...there are many variations for fat staining. Imho...most are histochemical mythology. Osmium "stains" fat...histologically. As do the conventional fat-soluble dyes when using frozen sections. I would only listen to alternatives/disagreements from JKiernan. I am still waiting for the next "generation" Histo person..... Cook, Kiernan.....who are the other Seminals?? Enquiringly Carl ________________________________________ From: Elizabeth Chlipala Sent: 26 March 2016 15:58 To: Joanna; Rene J Buesa Cc: Hobbs, Carl; histonet Subject: RE: [Histonet] Porcessing FFPE tissue without alcohol?? We use osmium post fixation to look at fat all of the time in mouse liver, nerve and muscle samples. It works well, sample size needs to be thin, samples are friable and can crack easily. We use a specific procedure for this it includes potassium dichromate I think, I'm at home but on Monday I can send the reference. One more thing don't trim into the block too much. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz at premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: Joanna via Histonet [histonet at lists.utsouthwestern.edu] Sent: Saturday, March 26, 2016 9:20 AM To: Rene J Buesa Cc: Hobbs, Carl; histonet Subject: Re: [Histonet] Porcessing FFPE tissue without alcohol?? How about Sudan Black stain? > On Mar 26, 2016, at 4:32 AM, Rene J Buesa via Histonet wrote: > > The only problem I see is that the fat will be preserved, as you wrote, as a black osmium oxidate but you will not be able to use any "standard" fat stain; otherwise it will work.Ren? > > On Friday, March 25, 2016 2:41 PM, "Hobbs, Carl via Histonet" wrote: > > > > > Fix the tissue in Formalin, wash well in dw, then place very small pieces into Osmium tetroxide solution ( std soln for TEM post-fixation) > Processing to Pwax as usual. > Basically, you will see lipids as black ( oxidised osmium) > That's the only way to demonstrate solvent- soluble lipids, using Pwax processing. > Sure, there are caveats but, in the main...it will be Ok, imho. > I invite comments as I may be doing exactly this very soon, to count myelinated nerve fibres in a sciatic nerve. > > > > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > 020 7848 6813 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SJMcCabe at phhealthcare.org Mon Mar 28 06:55:53 2016 From: SJMcCabe at phhealthcare.org (McCabe, Sara) Date: Mon, 28 Mar 2016 07:55:53 -0400 Subject: [Histonet] Cytology fixative In-Reply-To: References: Message-ID: <02AE2390303AAB43A823930EAD6B63AE03A4BA3C16@ex07> Wondering what everyone is using for their "wet" respiratory cytology specimens(bronch specimens, BAL, brushings etc) Currently we have our microbiology department use mucolytic agent. We also use cyto rich red for our brushing specimens. We are having a "drying out" effect and are wondering if these are causing this? Any info would be greatly appreciated! Sara J. McCabe, HT(ASCP)CM Senior Histotechnologist Penn Highlands DuBois 100 Hospital Avenue DuBois, PA 15801 814-375-3264 Telephone 814-375-3784 Fax sjmccabe at phhealthcare.org www.phhealthcare.org ________________________________________ This email and any attached files are sensitive in nature and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of Penn Highlands Healthcare or its affiliates.. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. From eddessa at emory.edu Mon Mar 28 08:36:08 2016 From: eddessa at emory.edu (Dessasau III, Evan) Date: Mon, 28 Mar 2016 13:36:08 +0000 Subject: [Histonet] Processing FFPE tissue without alcohol In-Reply-To: <153b3daac70.27e6.145c840c2545cc8d226fb8e47258f83d@sc.rr.com> References: <153b3daac70.27e6.145c840c2545cc8d226fb8e47258f83d@sc.rr.com> Message-ID: Will they be able to do IHC stains? -----Original Message----- From: Wanda Shotsberger Gray via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Saturday, March 26, 2016 12:56 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Processing FFPE tissue without alcohol While the tissue will still go through alcohol, have you considered preserving the fat with osmium tetroxide prior to routine processing? This turns the fat black, but it is retained in the tissue. Hi Histonet, is there a way to process tissue for paraffin embedding without using alcohol? One of the labs that send their processing to us will be doing a study examining fat. I told them if they want to look at the fat they will have to cut frozen sections but I'm being ask again about processing without the alcohol. So I said I would ask around. Please let me know what you think. Thank you for your help! E-van Sent with AquaMail for Android http://www.aqua-mail.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From liz at premierlab.com Mon Mar 28 10:42:58 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Mon, 28 Mar 2016 09:42:58 -0600 Subject: [Histonet] Porcessing FFPE tissue without alcohol?? In-Reply-To: References: <1076021560.1008554.1458991976574.JavaMail.yahoo@mail.yahoo.com>, <893C1E6D-057B-4F37-A483-E897B58D277F@gmail.com>, <14E2C6176416974295479C64A11CB9AE02BEAFF7B4BA@SBS2K8.premierlab.local> Message-ID: <14E2C6176416974295479C64A11CB9AE02BEC2AD5C1A@SBS2K8.premierlab.local> Carl We have tried multiple procedures, there are procedures that use a combination of 2% osmium and 5% postassium dichromate, ones that call for shorter periods of time in Osmium and then periodic acid rinse post osmium - this is referenced in Freida's book - Histotechnology a Self-Instructional Text. We ultimately settled on a modification of different methods. We use a 1% osmium solution for 24 hours rinse well in water and process same day short cycle (20 minutes per station with propar instead of xylene). I'll post some images and our procedure on the block. Sectioning and staining of these samples is tricky and we have found at least in our hands that the tissue is very friable (liver primarily, sciatic nerve and muscle samples turned out better) and does not stay on the slides well so we have not been able to even counterstain with H&E without considerable tissue loss. Sections can be a bit uneven which is OK for routine viewing via a microscope but not good for whole slide scanning and image analysis applications. I have pics of all of the problems associated with this protocol along with some really nice ones. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Hobbs, Carl [mailto:carl.hobbs at kcl.ac.uk] Sent: Saturday, March 26, 2016 12:56 PM To: Elizabeth Chlipala; Joanna; Rene J Buesa Cc: histonet Subject: Re: [Histonet] Porcessing FFPE tissue without alcohol?? Thanks, Liz. If you look at fat all the time, using Osmium.....you then are not sure if you use K-dichromate? I am a tad confused Also....why not trim the block too much? Best wishes, Carl NB: Rene stated that I wouldn't be able to use any other fat stains...that's the point, Rene. I don't need any other. I commit to Osmium. Yep...there are many variations for fat staining. Imho...most are histochemical mythology. Osmium "stains" fat...histologically. As do the conventional fat-soluble dyes when using frozen sections. I would only listen to alternatives/disagreements from JKiernan. I am still waiting for the next "generation" Histo person..... Cook, Kiernan.....who are the other Seminals?? Enquiringly Carl ________________________________________ From: Elizabeth Chlipala Sent: 26 March 2016 15:58 To: Joanna; Rene J Buesa Cc: Hobbs, Carl; histonet Subject: RE: [Histonet] Porcessing FFPE tissue without alcohol?? We use osmium post fixation to look at fat all of the time in mouse liver, nerve and muscle samples. It works well, sample size needs to be thin, samples are friable and can crack easily. We use a specific procedure for this it includes potassium dichromate I think, I'm at home but on Monday I can send the reference. One more thing don't trim into the block too much. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz at premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: Joanna via Histonet [histonet at lists.utsouthwestern.edu] Sent: Saturday, March 26, 2016 9:20 AM To: Rene J Buesa Cc: Hobbs, Carl; histonet Subject: Re: [Histonet] Porcessing FFPE tissue without alcohol?? How about Sudan Black stain? > On Mar 26, 2016, at 4:32 AM, Rene J Buesa via Histonet wrote: > > The only problem I see is that the fat will be preserved, as you > wrote, as a black osmium oxidate but you will not be able to use any > "standard" fat stain; otherwise it will work.Ren? > > On Friday, March 25, 2016 2:41 PM, "Hobbs, Carl via Histonet" wrote: > > > > > Fix the tissue in Formalin, wash well in dw, then place very small > pieces into Osmium tetroxide solution ( std soln for TEM post-fixation) Processing to Pwax as usual. > Basically, you will see lipids as black ( oxidised osmium) That's the > only way to demonstrate solvent- soluble lipids, using Pwax processing. > Sure, there are caveats but, in the main...it will be Ok, imho. > I invite comments as I may be doing exactly this very soon, to count myelinated nerve fibres in a sciatic nerve. > > > > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > 020 7848 6813 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ihcworkshop at gmail.com Mon Mar 28 14:12:02 2016 From: ihcworkshop at gmail.com (Ihc Workshop) Date: Mon, 28 Mar 2016 12:12:02 -0700 Subject: [Histonet] IHC Wet WORKSHOP Announcement Message-ID: Hands-on IHC Training Course; June 23 & 24, 2016 San Francisco Bay Area. Space limited to 15 people......For details; Email Maria Ihcworkshop at gmail.com From taylor at prometheushealthcare.com Mon Mar 28 15:34:51 2016 From: taylor at prometheushealthcare.com (Taylor Rinaldi) Date: Mon, 28 Mar 2016 16:34:51 -0400 Subject: [Histonet] **RECRUITER** Histology Bench and Management Opportunities nationwide Message-ID: <034701d18931$48647140$d92d53c0$@prometheushealthcare.com> Hello all! My name is Taylor Rinaldi, Recruiting Manager at Prometheus healthcare. We specialize specifically in laboratory recruiting all over the United States for many hospitals and reference laboratories. We are currently working on a few new orders for multiple opportunities with well-known laboratories in the New York and New Jersey area. We are recruiting for BENCH, SUPERVISORY AND MANAGEMENT candidates for these different lab locations. These positions are fulltime, permanent opportunities. ASCP certification preferred. Relocation assistance offered. If you may be interested in this opportunity or would like to hear about our nationwide histotech openings, please reach out to me for immediate consideration. States we are currently recruiting for in histology: Georgia Texas California New York New jersey Florida Mississippi Thank you in advance! Taylor Rinaldi Recruiting Manager Prometheus Healthcare Office (301) 693-9057 Taylor at prometheushealthcare.com From tony.henwood at health.nsw.gov.au Mon Mar 28 17:36:06 2016 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon, 28 Mar 2016 22:36:06 +0000 Subject: [Histonet] Porcessing FFPE tissue without alcohol?? In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF0148125C@SVDCMBX-MEX008.nswhealth.net> You might find these articles to be useful Tracy, R. E., & Walia, P. (2002). A method to fix lipids for staining fat embolism in paraffin sections. Histopathology, 41(1), 75-79. Turello, R., Snyder, D., & Hartman, H. A. (1984). A modification the osmium tetroxide post-fixation technique for the demonstration of extracellular lipid in paraffin-embedded tissue sections. Journal of Histotechnology, 7(2), 75-77. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Hobbs, Carl via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Saturday, 26 March 2016 5:30 AM To: histonet Subject: Re: [Histonet] Porcessing FFPE tissue without alcohol?? Fix the tissue in Formalin, wash well in dw, then place very small pieces into Osmium tetroxide solution ( std soln for TEM post-fixation) Processing to Pwax as usual. Basically, you will see lipids as black ( oxidised osmium) That's the only way to demonstrate solvent- soluble lipids, using Pwax processing. Sure, there are caveats but, in the main...it will be Ok, imho. I invite comments as I may be doing exactly this very soon, to count myelinated nerve fibres in a sciatic nerve. Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL ? 020 7848 6813 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From LRaff at uropartners.com Tue Mar 29 07:37:50 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 29 Mar 2016 12:37:50 +0000 Subject: [Histonet] How Does Your Lab Keep Clean and Bright (blog post) Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B7B033A@COLOEXCH01.uropartners.local> http://www.chicagonow.com/downsize-maybe/2016/03/can-newman-and-redford-paint-our-lab-or-fund-our-house/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From kcastillo at swskin.net Tue Mar 29 07:40:33 2016 From: kcastillo at swskin.net (Kristy Castillo) Date: Tue, 29 Mar 2016 05:40:33 -0700 Subject: [Histonet] HT Exam Message-ID: How would I be able to get a example test for the HT exam? Is there a place that you can go to get example questions? Know someone studying for the exam, but she is not sure what to study and is really nervous. Thanks Histonetters! Kristy Castillo ________________________________ This transmission may contain confidential information, some or all of which may be protected health information as defined by the federal Health Insurance Portability & Accountability Act (HIPAA) Privacy Rule. This transmission is intended for the exclusive use of the individual or entity to whom it is addressed and may contain information that is proprietary, privileged, confidential and/or exempt from disclosure under applicable law. If you are not the intended recipient (or an employee or agent responsible for delivering this transmission to the intended recipient), you are hereby notified that any disclosure, dissemination, distribution or copying of this information is strictly prohibited and may be subject to legal restriction or sanction. Please notify the sender by telephone to arrange the return or destruction of the information and all copies. From brett_connolly at merck.com Tue Mar 29 08:17:49 2016 From: brett_connolly at merck.com (Connolly, Brett M) Date: Tue, 29 Mar 2016 09:17:49 -0400 Subject: [Histonet] HT Exam In-Reply-To: References: Message-ID: For $30.00 you can get them from ASCP....probably a good investment !! http://www.starttest.com/7.2.0.0/cart.aspx?program=ASCPPractice Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: Kristy Castillo via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, March 29, 2016 8:41 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] HT Exam How would I be able to get a example test for the HT exam? Is there a place that you can go to get example questions? Know someone studying for the exam, but she is not sure what to study and is really nervous. Thanks Histonetters! Kristy Castillo ________________________________ This transmission may contain confidential information, some or all of which may be protected health information as defined by the federal Health Insurance Portability & Accountability Act (HIPAA) Privacy Rule. This transmission is intended for the exclusive use of the individual or entity to whom it is addressed and may contain information that is proprietary, privileged, confidential and/or exempt from disclosure under applicable law. If you are not the intended recipient (or an employee or agent responsible for delivering this transmission to the intended recipient), you are hereby notified that any disclosure, dissemination, distribution or copying of this information is strictly prohibited and may be subject to legal restriction or sanction. Please notify the sender by telephone to arrange the return or destruction of the information and all copies. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Mari.Yang at Hoag.org Tue Mar 29 16:28:02 2016 From: Mari.Yang at Hoag.org (Yang, Mari) Date: Tue, 29 Mar 2016 14:28:02 -0700 Subject: [Histonet] Medical Laboratory Survey Invitation In-Reply-To: <02A9B6BBE70204458C12BF79FEEF018C11050033@HHEXMB03.hoag.org> References: <02A9B6BBE70204458C12BF79FEEF018C11050033@HHEXMB03.hoag.org> Message-ID: <02A9B6BBE70204458C12BF79FEEF018C110500D6@HHEXMB03.hoag.org> Dear Histonet, It's not too late to participate in the survey, if you have not already done so. Thank you in advance for participating in this meaningful study for the Medical Laboratory. Best regards, Mari Yang, MHA, CT(ASCP)HTL Survey Link: https://www.surveymonkey.com/r/77X9X58 From: Yang, Mari Sent: Thursday, March 24, 2016 5:11 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Medical Laboratory Survey Invitation Dear Histonet, My name is Mari Yang and I am a doctorate student pursuing a degree in Management and Organizational Leadership. I have the unique opportunity to facilitate a study exploring the extent to which spiritual leadership positively influences organizational commitment, productivity, life satisfaction, and negatively influences burnout in the medical laboratory. I invite all persons over the age of 18 who are currently working in a medical laboratory in the US to participate. I am passionate about this study because I believe the findings will add value to the medical laboratory profession. Thank you for taking the time to participate. The public announcement for this study is posted on the ASCP Lab Forum http://bit.ly/224IwVE or you may go directly to the survey https://www.surveymonkey.com/r/77X9X58 Thank you in advance for your contribution. Best regards, Mari Yang, MHA, CT(ASCP)HTL P Save a tree, please don't print this e-mail unless you really need to. Please note that the information contained in this message and any files transmitted with it are privileged and confidential and are protected from disclosure under the law, including the Health Insurance Portability and Accountability Act (HIPAA). If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited and may subject you to criminal or civil penalties. If you have received this communication in error, please notify the sender by replying to the message and delete the material from any computer. Thank you, Hoag Memorial Hospital Presbyterian and its Affiliates From emartinez2 at echd.org Wed Mar 30 14:43:29 2016 From: emartinez2 at echd.org (Estela Martinez) Date: Wed, 30 Mar 2016 19:43:29 +0000 Subject: [Histonet] (no subject) Message-ID: Hello My Histo Friends, Does anybody know if OSHA has separate giude lines for Histology? We are having to wear gloves and face shields when cutting and also special shoes in the gross area because of knifes and blades. The main lab has to do all this per OSHA and was wondering if there are separate guide lines for Histology Department. Can somebody please help me! Thanks in advance!! Estela Martinez Histology Supervisor Medical Center Hospital Odessa, TX 79761 432-640-2348 emartinez2 at echd.org CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party without permission of original user and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From LRaff at uropartners.com Thu Mar 31 08:03:47 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 31 Mar 2016 13:03:47 +0000 Subject: [Histonet] Health Related Follow Up Blog Post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B7B29F0@COLOEXCH01.uropartners.local> Men, take notice! Women, share with any men in your life! http://www.chicagonow.com/downsize-maybe/2016/03/medicare-gets-this-one-right-for-now/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From BZIMMERM at gru.edu Thu Mar 31 13:13:43 2016 From: BZIMMERM at gru.edu (Zimmerman, Billie) Date: Thu, 31 Mar 2016 18:13:43 +0000 Subject: [Histonet] Georgia Society for Histotechnology Histopalooza 2016 Message-ID: This year's meeting at Lake Lanier Legacy Lodge falls on the weekend of the Full Moon Party. This event happens at Sunset Cove on every full moon. There will be a DJ from 3pm to 7pm followed by a live band from 7pm to 11pm. The band is Fly Betty. Look them up at www.flybettyband There will be outdoor fire pits, food and beverage specials and fireworks on the beach. This will be Saturday April 23rd. So, come to Lake Lanier for your continuing ed, and howl at the moon on Saturday night. If you see Joe Mangiello anywhere tell him I'll be there to brush his coat, take him for a walk or whatever. See you in a few weeks, Billie Zimmerman ARH-WOOOOOOOOOOOOO