[Histonet] CD3 antibody for FFPE mouse tissue

Caroline Miller mills at 3scan.com
Tue Jun 21 16:39:19 CDT 2016


+1 to the Dako antibody, it is the only one I have ever found to work (but
I have never used them on the automated staining systems, only old-skool by
hand or sequenza)

mills

On Tue, Jun 21, 2016 at 8:19 AM, Elizabeth Chlipala via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Brett
>
> Dako's rabbit anti human CD3 cross reacts with mouse, or where you looking
> for a CD3 that only detects mouse?
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 881-0763 cell
> (303) 682-9060 fax
> liz at premierlab.com
>
> Ship to address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
> ________________________________________
> From: Connolly, Brett M via Histonet [histonet at lists.utsouthwestern.edu]
> Sent: Tuesday, June 21, 2016 7:59 AM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] CD3 antibody for FFPE mouse tissue
>
> Hi all,
>
> Looking for recommendation for an anti-mouse CD3 antibody for FFPE
> sections.
>
> I have some old Histonet messages referring to a Neomarkers RB-360 ab
> which I found through Thermo, but I would like to hear about more recent
> experiences/recommendations....
>
> Thanks as always,
> Brett
>
> Brett M. Connolly, Ph.D.
> Prin. Scientist,
> Translational Biomarkers - Imaging
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> brett_connolly at merck.com<mailto:brett_connolly at merck.com>
> T- 215-652-2501
> F- 215-993-6803
>
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-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297


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