From CDavis at che-east.org Wed Jun 1 06:29:18 2016 From: CDavis at che-east.org (Cassie P. Davis) Date: Wed, 1 Jun 2016 11:29:18 +0000 Subject: [Histonet] Static electricity Tip Message-ID: <5C815EADE724D14AA0CC8F037C4185F079B2FDE1@SB01MSTMBX13.sb.trinity-health.org> I cannot be the only histology tech that had a problem with static electricity so I am sharing my recent find. In the past I had tried extra fabric softener, dryer sheets at microtomy, wool balls in my dryer with my uniforms, any suggestions, I tried with little to no success. The recent try was a large ball of aluminum foil (size of a baseball) in the dryer instead for dryer sheets or dryer ball with my uniforms (thank you YOUTUBE) and much to my surprise this was the first time I was able to go through work without my lab coat or microtomy sections sticking to me. Yes, you can reuse it and yes, it does make a thudding sound in the dryer that might drive some crazy but no where near as crazy as the static at microtomy. Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Shannon.Logan at bellin.org Wed Jun 1 10:53:40 2016 From: Shannon.Logan at bellin.org (Logan, Shannon) Date: Wed, 1 Jun 2016 15:53:40 +0000 Subject: [Histonet] Static electricity Tip In-Reply-To: <5C815EADE724D14AA0CC8F037C4185F079B2FDE1@SB01MSTMBX13.sb.trinity-health.org> References: <5C815EADE724D14AA0CC8F037C4185F079B2FDE1@SB01MSTMBX13.sb.trinity-health.org> Message-ID: <8ce29df57956478dbc7fee3d265d6037@BAPWEXCH001a.bellin.com> Glad to see this post....I also use the dryer balls too (two tennis balls) and it helps greatly!! No more static and cutting is much more "tame". Not to mention that it is healthier because dryer sheets are full of chemicals and toxins. Have a good one... Shannon H. Logan B.S. HTL (ASCP) Pathology Dept. Bellin Health 744 S Webster Ave Green Bay, WI 54305 920-433-3653 -----Original Message----- From: Cassie P. Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, June 01, 2016 6:29 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Static electricity Tip I cannot be the only histology tech that had a problem with static electricity so I am sharing my recent find. In the past I had tried extra fabric softener, dryer sheets at microtomy, wool balls in my dryer with my uniforms, any suggestions, I tried with little to no success. The recent try was a large ball of aluminum foil (size of a baseball) in the dryer instead for dryer sheets or dryer ball with my uniforms (thank you YOUTUBE) and much to my surprise this was the first time I was able to go through work without my lab coat or microtomy sections sticking to me. Yes, you can reuse it and yes, it does make a thudding sound in the dryer that might drive some crazy but no where near as crazy as the static at microtomy. Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jblanchard at reliapath.com Wed Jun 1 12:34:33 2016 From: jblanchard at reliapath.com (Jeanne Blanchard) Date: Wed, 1 Jun 2016 17:34:33 +0000 Subject: [Histonet] position opening Message-ID: Hi everyone. I'm the Histology Supervisor for an independent Pathology lab located in South Louisiana. We're currently looking for a Histotechnologist to fill a 1am - 9am shift. It's a full time position with full benefits (401K, insurance). If anyone is interested, please email (jblanchard at reliapath.com) or call me at the office (see number below). Jeanne Blanchard, CT (ASCP)CM 337-233-1899 From vlmckaughan at ccmh.org Wed Jun 1 12:53:37 2016 From: vlmckaughan at ccmh.org (Vicki McKaughan) Date: Wed, 1 Jun 2016 17:53:37 +0000 Subject: [Histonet] Position opening WV Message-ID: We currently have a full time histotech position in Parkersburg, WV. Shift is Mon-Fri 530-2p with some variability in times. Week-ends and holidays are covered with call. If you would like more information please contact me Vicki McKaughan Histology Supervisor Camden Clark Medical Center vlmckaughan at ccmh.org phone 304-424-2304 fax 304-424-2716 [cid:image001.jpg at 01D147D6.11FAAF30] From brett_connolly at merck.com Wed Jun 1 12:56:25 2016 From: brett_connolly at merck.com (Connolly, Brett M) Date: Wed, 1 Jun 2016 13:56:25 -0400 Subject: [Histonet] HSV-TK antibody for IHC Message-ID: Anyone know of an anti-HSV tyrosine kinase antibody suitable for IHC? The ones I am finding are WB and ELISA only. Thanks, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From classicdoc at gmail.com Wed Jun 1 13:04:26 2016 From: classicdoc at gmail.com (Douglas Gregg) Date: Wed, 1 Jun 2016 14:04:26 -0400 Subject: [Histonet] Histonet Digest, Vol 151, Issue 1 In-Reply-To: References: Message-ID: Subject: Re: [Histonet] Histology tips Here is one that your safety officer will like. Ether is sometimes called for as a de-stainer but is dangerous to keep except in a sealed can. Once opened, it can be quite explosive in a frig. This is my solution. Get a cheap can of engine starting fluid. It is pure ether. Don't get the high priced version with lubricants added. You just use what you need from the spray can and the rest keeps just fine for years. Douglas Gregg DVM PhD Veterinary pathologist Southold, NY On Wed, Jun 1, 2016 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Histology tips (Rene J Buesa) > 2. Subject: Re: FFPE human tissue blocks (carla.thompson at vwr.com) > 3. Re: Histonet Digest, Vol 150, Issue 31 5. Re: those > slippery lab floors (Normington Lacy) (Steve McClain) > 4. Static electricity Tip (Cassie P. Davis) > 5. Re: Static electricity Tip (Logan, Shannon) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 31 May 2016 17:36:35 +0000 (UTC) > From: Rene J Buesa > To: Charles Riley , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Histology tips > Message-ID: > <1388531700.1855321.1464716195815.JavaMail.yahoo at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > Would you share what you receive for the amusement/benefit of us all?Ren? > > On Tuesday, May 31, 2016 9:15 AM, Charles Riley via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > I am trying to do a histology tip of the week for my new histo team as a > way to help them learn some new ways to do troubleshooting. I've run out of > ideas at the moment. If anyone has any fun, interesting, or extremely > useful tip for troubleshooting any issues please send them to me. I would > really appreciate it. > > I also just want to add in a thank you for all the help I have received > from all our users. Everyone has been so helpful and I have been able to > fix a large amount of errors my predecessors has left piled up for me to > fix. > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 2 > Date: Tue, 31 May 2016 15:38:08 -0400 > From: carla.thompson at vwr.com > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Subject: Re: FFPE human tissue blocks > Message-ID: > > > Content-Type: text/plain; charset="UTF-8" > > Thanks so much, Theresa. I'll give you a call today. I appreciate your > help. > > Carla > ******************************************** > Carla Thompson > Senior Specimen Buyer > Microscope Slides Lab > > > > > > > > > > > > a division of VWR International, LLC. > 5100 West Henrietta Road > Post Office Box 92912 > Rochester, New York 14692-9012 > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 31 May 2016 21:48:42 +0000 > From: Steve McClain > To: "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Histonet Digest, Vol 150, Issue 31 5. Re: > those slippery lab floors (Normington Lacy) > Message-ID: > <012ADA4B5CC00F4AB5E4BAA399E0A5DFC043B0BE at ML1.McClainLabs.local> > Content-Type: text/plain; charset="us-ascii" > > I did see many slip and fall injuries in histolabs over the years. > > When we built this lab we accounted for that > and deliberately left the bare concrete rough, > and professionally painted polyurethane > of the type used in firehouse floors. > > Has held up reasonably well. > No slips or falls in 12 years. > Some stains; > Would not use in grossing room again however. > Drop a specimen fragment on that floor and you may be searching for an > hour. - > > Steve A. McClain, MD > 631 361 4000 > > > > ------------------------------ > > Message: 4 > Date: Wed, 1 Jun 2016 11:29:18 +0000 > From: "Cassie P. Davis" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Static electricity Tip > Message-ID: > < > 5C815EADE724D14AA0CC8F037C4185F079B2FDE1 at SB01MSTMBX13.sb.trinity-health.org > > > > Content-Type: text/plain; charset="iso-8859-1" > > I cannot be the only histology tech that had a problem with static > electricity so I am sharing my recent find. > > In the past I had tried extra fabric softener, dryer sheets at microtomy, > wool balls in my dryer with my uniforms, any suggestions, I tried with > little to no success. The recent try was a large ball of aluminum foil > (size of a baseball) in the dryer instead for dryer sheets or dryer ball > with my uniforms (thank you YOUTUBE) and much to my surprise this was the > first time I was able to go through work without my lab coat or microtomy > sections sticking to me. Yes, you can reuse it and yes, it does make a > thudding sound in the dryer that might drive some crazy but no where near > as crazy as the static at microtomy. > > > Cassandra Davis > Histology Technician > Anatomical Pathology Laboratory > Saint Francis Healthcare > 701 N. Clayton Street > Wilmington,DE 19805 > Office: 302-575-8095 > Email: CDavis at che-east.org > www.saintfrancishealthcare.org > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > > > ------------------------------ > > Message: 5 > Date: Wed, 1 Jun 2016 15:53:40 +0000 > From: "Logan, Shannon" > To: "Cassie P. Davis" > Cc: "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Static electricity Tip > Message-ID: <8ce29df57956478dbc7fee3d265d6037 at BAPWEXCH001a.bellin.com> > Content-Type: text/plain; charset="us-ascii" > > Glad to see this post....I also use the dryer balls too (two tennis balls) > and it helps greatly!! No more static and cutting > is much more "tame". > Not to mention that it is healthier because dryer sheets are full of > chemicals and toxins. > Have a good one... > > > Shannon H. Logan B.S. HTL (ASCP) > Pathology Dept. > Bellin Health > 744 S Webster Ave > Green Bay, WI 54305 > 920-433-3653 > > > > -----Original Message----- > From: Cassie P. Davis via Histonet [mailto: > histonet at lists.utsouthwestern.edu] > Sent: Wednesday, June 01, 2016 6:29 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Static electricity Tip > > I cannot be the only histology tech that had a problem with static > electricity so I am sharing my recent find. > > In the past I had tried extra fabric softener, dryer sheets at microtomy, > wool balls in my dryer with my uniforms, any suggestions, I tried with > little to no success. The recent try was a large ball of aluminum foil > (size of a baseball) in the dryer instead for dryer sheets or dryer ball > with my uniforms (thank you YOUTUBE) and much to my surprise this was the > first time I was able to go through work without my lab coat or microtomy > sections sticking to me. Yes, you can reuse it and yes, it does make a > thudding sound in the dryer that might drive some crazy but no where near > as crazy as the static at microtomy. > > > Cassandra Davis > Histology Technician > Anatomical Pathology Laboratory > Saint Francis Healthcare > 701 N. Clayton Street > Wilmington,DE 19805 > Office: 302-575-8095 > Email: CDavis at che-east.org > www.saintfrancishealthcare.org > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 151, Issue 1 > **************************************** > From mills at 3scan.com Wed Jun 1 13:31:39 2016 From: mills at 3scan.com (Caroline Miller) Date: Wed, 1 Jun 2016 11:31:39 -0700 Subject: [Histonet] Static electricity Tip In-Reply-To: <8ce29df57956478dbc7fee3d265d6037@BAPWEXCH001a.bellin.com> References: <5C815EADE724D14AA0CC8F037C4185F079B2FDE1@SB01MSTMBX13.sb.trinity-health.org> <8ce29df57956478dbc7fee3d265d6037@BAPWEXCH001a.bellin.com> Message-ID: There is also this thing, that works pretty well, made for record players: http://www.needledoctor.com/Milty-Zerostat-Gun mills On Wed, Jun 1, 2016 at 8:53 AM, Logan, Shannon via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Glad to see this post....I also use the dryer balls too (two tennis balls) > and it helps greatly!! No more static and cutting > is much more "tame". > Not to mention that it is healthier because dryer sheets are full of > chemicals and toxins. > Have a good one... > > > Shannon H. Logan B.S. HTL (ASCP) > Pathology Dept. > Bellin Health > 744 S Webster Ave > Green Bay, WI 54305 > 920-433-3653 > > > > -----Original Message----- > From: Cassie P. Davis via Histonet [mailto: > histonet at lists.utsouthwestern.edu] > Sent: Wednesday, June 01, 2016 6:29 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Static electricity Tip > > I cannot be the only histology tech that had a problem with static > electricity so I am sharing my recent find. > > In the past I had tried extra fabric softener, dryer sheets at microtomy, > wool balls in my dryer with my uniforms, any suggestions, I tried with > little to no success. The recent try was a large ball of aluminum foil > (size of a baseball) in the dryer instead for dryer sheets or dryer ball > with my uniforms (thank you YOUTUBE) and much to my surprise this was the > first time I was able to go through work without my lab coat or microtomy > sections sticking to me. Yes, you can reuse it and yes, it does make a > thudding sound in the dryer that might drive some crazy but no where near > as crazy as the static at microtomy. > > > Cassandra Davis > Histology Technician > Anatomical Pathology Laboratory > Saint Francis Healthcare > 701 N. Clayton Street > Wilmington,DE 19805 > Office: 302-575-8095 > Email: CDavis at che-east.org > www.saintfrancishealthcare.org > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From CIngles at uwhealth.org Wed Jun 1 20:04:08 2016 From: CIngles at uwhealth.org (Ingles Claire) Date: Thu, 2 Jun 2016 01:04:08 +0000 Subject: [Histonet] Histonet Digest, Vol 151, Issue 1 In-Reply-To: References: , Message-ID: Not to mention that if you inhale too many fumes you will pass out. One of the original anesthetics. :) Claire ________________________________________ From: Douglas Gregg via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, June 01, 2016 1:04 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 151, Issue 1 Subject: Re: [Histonet] Histology tips Here is one that your safety officer will like. Ether is sometimes called for as a de-stainer but is dangerous to keep except in a sealed can. Once opened, it can be quite explosive in a frig. This is my solution. Get a cheap can of engine starting fluid. It is pure ether. Don't get the high priced version with lubricants added. You just use what you need from the spray can and the rest keeps just fine for years. Douglas Gregg DVM PhD Veterinary pathologist Southold, NY From SohrabB1 at ah.org Thu Jun 2 15:02:24 2016 From: SohrabB1 at ah.org (Sohrab,Behnaz) Date: Thu, 2 Jun 2016 20:02:24 +0000 Subject: [Histonet] HIRE ? Message-ID: <92096F092E8CE54FBB5E272198FE5A7E2411767E@ahexcms001.ah.org> Any of the Histo Lab's requires Basic Life certificate for hireing in Histology ? ( with no Patient contact ) Behnaz Sohrab Regional Manager Anatomic pathology Behnaz.Sohrab at ah.org 323-268-5000 Ext.1711 323-265-5086 FAX From sbaldwin at mhhcc.org Fri Jun 3 08:09:26 2016 From: sbaldwin at mhhcc.org (Baldwin, Kathy) Date: Fri, 3 Jun 2016 13:09:26 +0000 Subject: [Histonet] SEND OUTS Message-ID: <9bb8e3e1bf7f45e987a75a60e7ea094e@exch1.mhhcc.org> Hi All Happy Friday Was wondering if anyone had a clear cut way on the send out books, my current transcriptionist is keeping them by ex Mayo send outs, Mutation analysis, Bone Marrows etc.. I thought the book should be alphabetical so all would be easier to find ?? Also have a place in our LIS Meditech but it is a little confusing?? Any ideas?? Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From LRaff at uropartners.com Fri Jun 3 08:58:15 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 3 Jun 2016 13:58:15 +0000 Subject: [Histonet] SEND OUTS In-Reply-To: <9bb8e3e1bf7f45e987a75a60e7ea094e@exch1.mhhcc.org> References: <9bb8e3e1bf7f45e987a75a60e7ea094e@exch1.mhhcc.org> Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10DE65BA@COLOEXCH01.uropartners.local> Your transcriptionist (or someone with a little Excel experience) should definitely set this up as a spread sheet. You would be able to search, sort, etc. Just be sure to back up on a daily basis! http://www.chicagonow.com/downsize-maybe/2016/06/american-ninja-warrior-presidential-style/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: Baldwin, Kathy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, June 03, 2016 8:09 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] SEND OUTS Hi All Happy Friday Was wondering if anyone had a clear cut way on the send out books, my current transcriptionist is keeping them by ex Mayo send outs, Mutation analysis, Bone Marrows etc.. I thought the book should be alphabetical so all would be easier to find ?? Also have a place in our LIS Meditech but it is a little confusing?? Any ideas?? Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz at premierlab.com Fri Jun 3 09:06:04 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Fri, 3 Jun 2016 08:06:04 -0600 Subject: [Histonet] Sakura Prisma 4740 combo film In-Reply-To: <248395418C8C074A83C7D06081C4F0164D0F9531@DERMLAB-SBS.dermlab.local> References: <15506ffeda9-2ec1-7748@webprd-a15.mail.aol.com> <14E2C6176416974295479C64A11CB9AE02BEC2AD6067@SBS2K8.premierlab.local> <248395418C8C074A83C7D06081C4F0164D0F9531@DERMLAB-SBS.dermlab.local> Message-ID: <14E2C6176416974295479C64A11CB9AE02BEC2AD60C7@SBS2K8.premierlab.local> In our situation the charcoal filters were not sufficient enough, we used xylene and it was not the tape but glass coverslipper. We tried it with just the carbon filters and it was not good enough. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Jonathan Jennings [mailto:JJennings at thedermlab.com] Sent: Friday, June 03, 2016 7:57 AM To: Elizabeth Chlipala Subject: RE: [Histonet] Sakura Prisma 4740 combo film Ours has 2 charcoal filters located behind the carousel rack on top of the coverslipper. I believe the filters have to be changed once per year. This would eliminate having to have external exhaust. We don't have any issues with fumes from our unit, but we also are using xylene substitute (SubX) inside the stainer, except for the last station before the slides go into the coverslipper. We use xylene in the last station of the stain line and xylene inside the coverslipper. Of course the only reason we are using xylene in these 2 stations is to activate the hydrocarbons in the tape coverslip film, as xylene substitute will not work for this. Jonathan Jennings Lab Manager 205.705.3550 205.705.3554 jjennings at thedermlab.com 3918 Montclair Rd, Ste 105 Birmingham, AL 35213 www.thedermlab.com This e-mail transmission, and any documents, files or previous e-mail messages attached to it, may contain confidential information. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of any of the information contained in or attached to this message is strictly prohibited. If you have received this transmission in error, please immediately notify?Jonathan Jennings by reply email or by telephone 1-855-705-1776, and destroy the original transmission and its attachments without reading them or saving them to any?media?storage device.? ? -----Original Message----- From: Elizabeth Chlipala via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 31, 2016 9:50 AM To: Patti Nelson - PNP Lab Consultant ; 'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu) Subject: Re: [Histonet] Sakura Prisma 4740 combo film Patti We have the Sakura Prisma with the Glas coverslipper and we have ours vented out. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Patti Nelson - PNP Lab Consultant via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, May 31, 2016 7:28 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Sakura Prisma 4740 combo film Hi everyone, I am considering placing a Sakura Prisma 4740 combo film in one of my labs. My question is , does the cover slip unit need an external exhaust. Please consider that the space the unit will be placed is small. Thank you in advance. Sincerely, PATTI NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR DGC/ZADEH LABS PO BOX 412 CABAZON, CA. 92230 909-841-9761 nelsonrnch at verizon.net CONFIDENTIALITY NOTICE:This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender ofthe delivery error by e-mail or you may call 909-841-9761. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmgriffey at ucdavis.edu Fri Jun 3 12:40:38 2016 From: rmgriffey at ucdavis.edu (Rebecca M Griffey) Date: Fri, 3 Jun 2016 17:40:38 +0000 Subject: [Histonet] New Job Posting Message-ID: Can you please post this Histology Laboratory Supervisor position on your Histo Net? Histology Laboratory Supervisor position at UC Davis - www.employment.ucdavis.edu/applicants/Central?quickFind=73367 Thank you, Becky From sandoval66 at sbcglobal.net Fri Jun 3 12:40:26 2016 From: sandoval66 at sbcglobal.net (Elia Sandoval) Date: Fri, 3 Jun 2016 17:40:26 +0000 (UTC) Subject: [Histonet] Special stains control blocks template References: <1270966990.4802720.1464975626993.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1270966990.4802720.1464975626993.JavaMail.yahoo@mail.yahoo.com> Hello, I was wondering if any of you might be able to help me out. I'm searching for samples of a template/ form validation sheet that I can use for documentation and CAP inspection for control blocks for special stains. Thank you in advance From jaylundgren at gmail.com Fri Jun 3 19:53:45 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 3 Jun 2016 17:53:45 -0700 Subject: [Histonet] Static electricity Tip In-Reply-To: References: <5C815EADE724D14AA0CC8F037C4185F079B2FDE1@SB01MSTMBX13.sb.trinity-health.org> <8ce29df57956478dbc7fee3d265d6037@BAPWEXCH001a.bellin.com> Message-ID: They sell industrial anti-static sprays, you spray the equipment, the bottom of your shoes, and the floor. They work. http://www.amazon.com/ACL-Staticide-General-Purpose-Anti-Stat/dp/B00BR55KQ6/ref=zg_bs_3310249011_1 Also, you can ground your microtome to a pipe with a ground strap. On Wed, Jun 1, 2016 at 11:31 AM, Caroline Miller via Histonet < histonet at lists.utsouthwestern.edu> wrote: > There is also this thing, that works pretty well, made for record players: > > http://www.needledoctor.com/Milty-Zerostat-Gun > > mills > > On Wed, Jun 1, 2016 at 8:53 AM, Logan, Shannon via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Glad to see this post....I also use the dryer balls too (two tennis > balls) > > and it helps greatly!! No more static and cutting > > is much more "tame". > > Not to mention that it is healthier because dryer sheets are full of > > chemicals and toxins. > > Have a good one... > > > > > > Shannon H. Logan B.S. HTL (ASCP) > > Pathology Dept. > > Bellin Health > > 744 S Webster Ave > > Green Bay, WI 54305 > > 920-433-3653 > > > > > > > > -----Original Message----- > > From: Cassie P. Davis via Histonet [mailto: > > histonet at lists.utsouthwestern.edu] > > Sent: Wednesday, June 01, 2016 6:29 AM > > To: histonet at lists.utsouthwestern.edu > > Subject: [Histonet] Static electricity Tip > > > > I cannot be the only histology tech that had a problem with static > > electricity so I am sharing my recent find. > > > > In the past I had tried extra fabric softener, dryer sheets at microtomy, > > wool balls in my dryer with my uniforms, any suggestions, I tried with > > little to no success. The recent try was a large ball of aluminum foil > > (size of a baseball) in the dryer instead for dryer sheets or dryer ball > > with my uniforms (thank you YOUTUBE) and much to my surprise this was the > > first time I was able to go through work without my lab coat or microtomy > > sections sticking to me. Yes, you can reuse it and yes, it does make a > > thudding sound in the dryer that might drive some crazy but no where near > > as crazy as the static at microtomy. > > > > > > Cassandra Davis > > Histology Technician > > Anatomical Pathology Laboratory > > Saint Francis Healthcare > > 701 N. Clayton Street > > Wilmington,DE 19805 > > Office: 302-575-8095 > > Email: CDavis at che-east.org > > www.saintfrancishealthcare.org > > > > Confidentiality Notice: > > This e-mail, including any attachments is the property of Trinity Health > > and is intended for the sole use of the intended recipient(s). It may > > contain information that is privileged and confidential. Any > unauthorized > > review, use, disclosure, or distribution is prohibited. If you are not > the > > intended recipient, please delete this message, and reply to the sender > > regarding the error in a separate email. > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Caroline Miller (mills) > Director of Histology > 3Scan.com > 415 2187297 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From j.rowaihi at alborglaboratories.com Sun Jun 5 10:43:12 2016 From: j.rowaihi at alborglaboratories.com (Jamal Rwaihi) Date: Sun, 5 Jun 2016 18:43:12 +0300 Subject: [Histonet] ANP.22970 Annual Result Comparison Message-ID: <000301d1bf40$f8c4bc30$ea4e3490$@alborglaboratories.com> Good day colleagues I need your help in collecting the data for the bellow requirements for CAP, is their good reference to collect the benchmark statistics for ER, PR & Her2neu ? ANP.22970 Annual Result Comparison For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. NOTE: Individuals interpreting the assay must also have their concordance compared with each other and this concordance should also be at least 95%. With specific reference to estrogen and progesterone receptor studies: in general, the overall proportion of ER-negative breast cancers (invasive and DCIS) should not exceed 30%. The proportion is somewhat lower in postmenopausal than premenopausal women (approximately 20% vs. 35%). The proportion is considerably lower in well-differentiated carcinomas (<10%) and certain special types of invasive carcinomas (<10% in lobular, tubular, and mucinous types). The proportion of PgR-negative cases is 10-15% higher than for ER-negative in each of these settings. Investigation is warranted if the proportion of negative cases is significantly lower in any of these settings. Jamal M. Al Rowaihi BCs, HT, Anatomic Pathology Supervisor Al Borg Medical Laboratories From gayle.callis at bresnan.net Mon Jun 6 11:27:01 2016 From: gayle.callis at bresnan.net (Gayle Callis) Date: Mon, 6 Jun 2016 11:27:01 -0500 Subject: [Histonet] NSH Journal of Histotechnology reminder on call of manuscripts for troubleshooting stains due July 1st Message-ID: <000a01d1c010$470a1d70$d51e5850$@bresnan.net> Dear Histonetters, This is a reminder for people to submit a manuscript on troubleshooting H&E and special stains on paraffin embedded tissue sections. You do not have to be a member of NSH to submit manuscripts to the Journal of Histotechnology. JOH wants to know how you solve staining problems for both manual and automated staining for this special topics issue. This can be a short research communication previously known as a technical note. There is still time to meet the submission deadline, July 1, 2016. For instructions for authors and submission, go to http://www.editorialmanager.com/his/default.aspx. Inquiries can be directed to me or at JOH through contacts listed at under publications and Journal of Histotechnology at www.nsh.org website. Share you expertise with others in a JOH publication. Thank you Gayle M. Callis HTL/HT/MT(ASCP) Assistant Editor/Acting Editor, Special Issue on Troubleshooting Stains NSH Journal of Histotechnology From Kitty.Maxey at propath.com Mon Jun 6 11:53:02 2016 From: Kitty.Maxey at propath.com (Kitty Maxey) Date: Mon, 6 Jun 2016 16:53:02 +0000 Subject: [Histonet] Unsubscribe Message-ID: Kitty Maxey Director, Human Resources ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247-4914 214-237-1608 (direct) 214-237-1808 (fax) 1-800-258-1253, ext. 1608 (toll-free) Don't Follow the Leader. Join the Leader! To learn more about ProPath and careers, please visit http://www.ProPath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From rachel at gbi-inc.com Mon Jun 6 12:15:13 2016 From: rachel at gbi-inc.com (Rachel M Gonzalez) Date: Mon, 6 Jun 2016 13:15:13 -0400 Subject: [Histonet] retaining cut slides to keep or not to keep Message-ID: Hi everyone, I work in a research lab and have a number of slides that have been cut by the previous person in this position. Several slides are over one year. I would like to know what is the standard practice for keeping cut slides? I have used them and they stain just fine but the question was brought up in a lab meeting. How long can you keep unbaked slides? Room temperature/4C How long can you keep baked slides? Room temperature/4C Thanks Rachel Gonzalez PhD From rjbuesa at yahoo.com Mon Jun 6 15:10:01 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 6 Jun 2016 20:10:01 +0000 (UTC) Subject: [Histonet] retaining cut slides to keep or not to keep In-Reply-To: References: Message-ID: <945936985.2001133.1465243801919.JavaMail.yahoo@mail.yahoo.com> It seems you have "baked" and "unbaked" slides.4?C storage is always more expensive and "baked" slides are keep very well at RT.I think your first step is to ask around who would like those specific slides.If they will be used in the future, "bake" those "unbaked" and store all at RTRen? On Monday, June 6, 2016 1:45 PM, Rachel M Gonzalez via Histonet wrote: Hi everyone, I work in a research lab and have a number of slides that have been cut by the previous person in this position. Several slides are over one year.? I would like to know what is the standard practice for keeping cut slides? I have used them and they stain just fine but the question was brought up in a lab meeting. How long can you keep unbaked slides? Room temperature/4C How long can you keep baked slides? Room temperature/4C Thanks Rachel Gonzalez PhD _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From litepath2000 at yahoo.com Mon Jun 6 15:46:46 2016 From: litepath2000 at yahoo.com (NYSHisto) Date: Mon, 6 Jun 2016 20:46:46 +0000 (UTC) Subject: [Histonet] TUNEL References: <792522342.6022921.1465246006193.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <792522342.6022921.1465246006193.JavaMail.yahoo@mail.yahoo.com> Hi Histonetters Is anyone using the Roche In Situ cell death detection kit (TUNEL) on Ventana platform? If yes, have you also experienced that back order issues?? Does anyone have alternative kit/method worked out? any help/guidance would be greatly appreciatedThanksLP From mailee15 at uwalumni.com Tue Jun 7 08:30:41 2016 From: mailee15 at uwalumni.com (Mai Lee) Date: Tue, 7 Jun 2016 08:30:41 -0500 Subject: [Histonet] Frozen Sections Message-ID: I work in a Mohs lab. We use bleach wipes to clean tabletops and brush handles. You can use Dispatch, Sani-cloth with Bleach, or Clorox Healthcare Bleach Germicidal wipes. From Nancy_Schmitt at pa-ucl.com Tue Jun 7 12:18:24 2016 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Tue, 7 Jun 2016 17:18:24 +0000 Subject: [Histonet] Pathology physician assistant Message-ID: <372f05fde97e4ce6bfc71824644879ee@mercury.wad.pa-ucl.com> Hello- Our facility has 6 pathologist and one PA; I am wondering if anyone has a job description they would be able to share. We are doing some restructuring and wondering how the PA fits in at other places and what tasks they are performing besides grossing specimens; this is the only place this PA has worked. I appreciate any input, Nancy Schmitt HT, MLT(ASCP) Pathology Support Services Manager Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From tbraud at holyredeemer.com Tue Jun 7 12:35:40 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 7 Jun 2016 17:35:40 +0000 Subject: [Histonet] stored unstained sections Message-ID: <48E053DDF6CE074DB6A7414BA05403F8078AFD@HRHEX02-HOS.holyredeemer.local> Cut slides stored at room temp seem to last quite a long time for routine stains, however, cut slides do demonstrate a loss of antigenicity for IHC staining, depending on the antibody. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Today's Topics: 1. retaining cut slides to keep or not to keep (Rachel M Gonzalez) Message: 1 Date: Mon, 6 Jun 2016 13:15:13 -0400 From: Rachel M Gonzalez Hi everyone, I work in a research lab and have a number of slides that have been cut by the previous person in this position. Several slides are over one year. I would like to know what is the standard practice for keeping cut slides? I have used them and they stain just fine but the question was brought up in a lab meeting. How long can you keep unbaked slides? Room temperature/4C How long can you keep baked slides? Room temperature/4C Thanks Rachel Gonzalez PhD From tgenade at gmail.com Tue Jun 7 12:42:16 2016 From: tgenade at gmail.com (Tyrone Genade) Date: Tue, 7 Jun 2016 12:42:16 -0500 Subject: [Histonet] mounting medium for DAB & AP chromogens Message-ID: Hello, I'm currently using an ancient bottle of xylene based mounting medium and want to get something better that will serve multiple purposes. In the past I had used entellan for DAB and routine histo stains (i.e. H&E) but then a student accidentally (recklessly?) used my Mowiol + n-propyl gallate mounting medium (that I use for fluorescence) for a PTAH stain and it is worked well, retaining the sharpness of the stain much longer than the entellan did. In the past I had used the Mowiol for DAB and that seemed to work well. I am now preparing to perform staining with AEC and Fastred that don't like the alcohol dehydration steps needed for a xylene mounting medium. Is there any reason why I can't simply use the Mowiol mounting medium for all my work? It isn't like the DAB is going to dissolve... I am using methyl green as a counter stain for my current work. The mowiol mounting medium is glycerol based ( http://cshprotocols.cshlp.org/content/2006/1/pdb.rec10255.full?text_only=true with n-propyl gallate instead of DABCO). Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From jvickroy at SpringfieldClinic.com Tue Jun 7 15:01:21 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Tue, 7 Jun 2016 20:01:21 +0000 Subject: [Histonet] Formaldehyde annual education Message-ID: <9B1A1501A800064397369BD8072E6BCA0656A2CF@E2K10DB.springfieldclinic.com> I seem to recall that we once had to document that we went over the hazards of formaldehyde to the staff annually. Does anyone still do this? I know the rules on monitoring but as I was going over a past procedure I saw that we used to reeducate the staff each year. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From Erin.Martin at ucsf.edu Wed Jun 8 10:18:51 2016 From: Erin.Martin at ucsf.edu (Martin, Erin) Date: Wed, 8 Jun 2016 15:18:51 +0000 Subject: [Histonet] Santa Cruz antibodies Message-ID: <24B7B291CC88D04AB663958E77A1F59D26AA49@ex09.net.ucsf.edu> Hi all! Santa Cruz Biotech has to discontinue all goat and rabbit antibody production: http://blogs.sciencemag.org/pipeline/archives/2016/05/23/trouble-at-santa-cruz-biotechnology I hope you are all having a great Tuesday! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. From koellingr at comcast.net Wed Jun 8 10:50:32 2016 From: koellingr at comcast.net (koellingr at comcast.net) Date: Wed, 8 Jun 2016 15:50:32 +0000 (UTC) Subject: [Histonet] Santa Cruz antibodies In-Reply-To: <24B7B291CC88D04AB663958E77A1F59D26AA49@ex09.net.ucsf.edu> References: <24B7B291CC88D04AB663958E77A1F59D26AA49@ex09.net.ucsf.edu> Message-ID: <262574128.14834973.1465401032010.JavaMail.zimbra@comcast.net> Pretty interesting Erin, thanks. I reread the Nature article I saw back in February detailing a bit about the complaints being investigated.? Among those complaints were notifications of goats with coyote bites and massive tumors (from where??).? Both of which could drastically affect polyclonal Ab production. Which took me back remembering on HistoNet years ago about "the sick CD31 producing goat"? or "the only CD31 producing goat had died"? and that is why the Ab was fickle.? But thousands of goats and rabbits have now disappeared from their facility?? I had long before that stopped using anything Santa Cruz as we had proved scientifically that an antibody we used from them was not marking what it should. ? What is the phrase?? Caveat emptor ? Ray Koelling lecturer, University of Washington Medical School-Spokane campus Spokane WA ? ----- Original Message ----- From: "Erin via Histonet Martin" To: "histonet" Sent: Wednesday, June 8, 2016 8:18:51 AM Subject: [Histonet] Santa Cruz antibodies Hi all! Santa Cruz Biotech has to discontinue all goat and rabbit antibody production: http://blogs.sciencemag.org/pipeline/archives/2016/05/23/trouble-at-santa-cruz-biotechnology I hope you are all having a great Tuesday! Erin Erin Martin, Histology Supervisor UCSF ?Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. ?Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. ?If you receive this in error please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas at biopath.org Wed Jun 8 11:14:01 2016 From: plucas at biopath.org (Paula) Date: Wed, 8 Jun 2016 09:14:01 -0700 Subject: [Histonet] P16 Message-ID: <003201d1c1a0$c8878db0$5996a910$@biopath.org> Hello, Our pathologist would like us to get the P16 antibody. We have the Bond III and we use ready to use antibodies. Leica doesn't carry this antibody and so I was hoping someone can tell me which vendor carries it and that I can order it from. Thanks in advance for your help Paula Lab manager for BioPath Medical Group in California From jblanchard at reliapath.com Wed Jun 8 11:35:38 2016 From: jblanchard at reliapath.com (Jeanne Blanchard) Date: Wed, 8 Jun 2016 16:35:38 +0000 Subject: [Histonet] P16 In-Reply-To: <003201d1c1a0$c8878db0$5996a910$@biopath.org> References: <003201d1c1a0$c8878db0$5996a910$@biopath.org> Message-ID: <33dfc3f165e14270ac17128c41475d1e@EXCH.reliapath.com> We use the p16 from Ventana/Roche, but of course it comes in their dispenser. Jeanne Blanchard, CT (ASCP)CM -----Original Message----- From: Paula via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, June 08, 2016 11:14 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] P16 Hello, Our pathologist would like us to get the P16 antibody. We have the Bond III and we use ready to use antibodies. Leica doesn't carry this antibody and so I was hoping someone can tell me which vendor carries it and that I can order it from. Thanks in advance for your help Paula Lab manager for BioPath Medical Group in California _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjdessoye at commonwealthhealth.net Wed Jun 8 12:14:35 2016 From: mjdessoye at commonwealthhealth.net (Dessoye, Michael) Date: Wed, 8 Jun 2016 17:14:35 +0000 Subject: [Histonet] P16 In-Reply-To: <003201d1c1a0$c8878db0$5996a910$@biopath.org> References: <003201d1c1a0$c8878db0$5996a910$@biopath.org> Message-ID: Ventana/Roche bought out MTM which had the patent, so they're the only vendor you can get it from. Since you're using Bond you obviously don't want it in a dispenser, but you can buy their manual staining kit and just use the antibody from that. Michael J. Dessoye, M.S.?|?Histology/Toxicology/RIA Supervisor?|?Commonwealth Health Laboratory Services |?mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 -----Original Message----- From: Paula [mailto:plucas at biopath.org] Sent: Wednesday, June 08, 2016 12:14 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] P16 Hello, Our pathologist would like us to get the P16 antibody. We have the Bond III and we use ready to use antibodies. Leica doesn't carry this antibody and so I was hoping someone can tell me which vendor carries it and that I can order it from. Thanks in advance for your help Paula Lab manager for BioPath Medical Group in California -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Julia.Cates at AHSS.ORG Wed Jun 8 12:29:38 2016 From: Julia.Cates at AHSS.ORG (Cates, Julia) Date: Wed, 8 Jun 2016 17:29:38 +0000 Subject: [Histonet] Formaldehyde annual education Message-ID: Jim, You are correct. OSHA requires annual education for any personnel that handle formalin to be educated in the hazards, proper handling, etc... This can be as simple or as involved as you want to make it but it should cover the basics at the very least. Thanks, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Park Ridge Health Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. From sbaldwin at mhhcc.org Wed Jun 8 12:31:18 2016 From: sbaldwin at mhhcc.org (Baldwin, Kathy) Date: Wed, 8 Jun 2016 17:31:18 +0000 Subject: [Histonet] P16 Message-ID: <5a87a0f06ba547048d5780f278396102@exch02.mhhcc.org> Just saw someone reaching out for P16 was wondering if anyone would share their protocol for this antibody we are using Ventana/Roche and are stain is coming out weak :( Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tbraud at holyredeemer.com Wed Jun 8 12:46:33 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 8 Jun 2016 17:46:33 +0000 Subject: [Histonet] Formaldehyde Education Message-ID: <48E053DDF6CE074DB6A7414BA05403F8078D87@HRHEX02-HOS.holyredeemer.local> Annual Formaldehyde safety education should be part of your on-boarding process, as well as annual education, per OSHA Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 4. Formaldehyde annual education (Vickroy, James) Message: 4 Date: Tue, 7 Jun 2016 20:01:21 +0000 From: "Vickroy, James" Subject: [Histonet] Formaldehyde annual education I seem to recall that we once had to document that we went over the hazards of formaldehyde to the staff annually. Does anyone still do this? I know the rules on monitoring but as I was going over a past procedure I saw that we used to reeducate the staff each year. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com ****************************** From craigak12 at gmail.com Wed Jun 8 13:42:15 2016 From: craigak12 at gmail.com (J B) Date: Wed, 08 Jun 2016 18:42:15 +0000 Subject: [Histonet] P16 In-Reply-To: <5a87a0f06ba547048d5780f278396102@exch02.mhhcc.org> References: <5a87a0f06ba547048d5780f278396102@exch02.mhhcc.org> Message-ID: Ventana medical systems is the only one I know of right now. On Wed, Jun 8, 2016, 10:47 AM Baldwin, Kathy via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Just saw someone reaching out for P16 was wondering if anyone would share > their protocol for this antibody we are using Ventana/Roche and are stain > is coming out weak :( > > > > Thanks > S. Kathy Baldwin > Histology/Cytology Supervisor > Memorial Hospital and Health Care Center > 800 West 9th St. > Jasper, Indiana 47546 > Office 812-996-0210 > Fax 812-996-0232 > > > > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information or otherwise protected by law. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not the > intended recipient, please contact the sender by reply e-mail and destroy > all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From b-frederick at northwestern.edu Wed Jun 8 14:07:22 2016 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Wed, 8 Jun 2016 19:07:22 +0000 Subject: [Histonet] CAP Message-ID: <09a38068d3b7437cabdfe0d021380e4a@evcspmbx03.ads.northwestern.edu> CAP Rant: 3 months to get here and still not here. Time frame should have ended May 30th. Black out days after that. Final date actually 10th. Called before Memorial day to say they couldn't come and could they come between 1-10th June. Now narrowed to this week. Not here. I am supposed to be off 10th,but not if they come as I am only tech. Inspectors not flying in, local to Chicago. And they can't get here? Has this happened to anyone? More tension since they have not showed up. People mad plans as these are blackout days. I'm over it, they can go find a lake and there is one real close. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu From mw at personifysearch.com Wed Jun 8 14:31:55 2016 From: mw at personifysearch.com (Matt Ward) Date: Wed, 8 Jun 2016 15:31:55 -0400 Subject: [Histonet] Histology Career Opportunities - Field Applications Specialist Message-ID: Hello Histonet, Our exclusively retained client has opened a new Applications Specialist opportunity and I wanted to reach out regarding a few specifics and see if anyone would be interested in learning more. Our client is a global leader in cancer diagnostics and they are looking for an Applications Specialist with a strong background and understanding of histology workflow and Laboratory Information Systems. This individual will provide technical training on use of systems and must be comfortable presenting to customers at all levels. The company is part of a $20 billion organization and growing. This position is a full-time, direct-hire role with competitive salary and full benefits. If you or anyone you know may be interested in learning more about this position, please contact me directly at *mw at personifysearch.com . * Thank you! Matt *Matt Ward* Program Manager, RPO *Personify* 401 Harrison Oaks Blvd, Suite 350 Cary, NC 27513 Direct Line: (919) 459-3654 Toll Free: (800) 875-6188 ext. 103 www.personifysearch.com https://www.linkedin.com/in/mattwardpersonify From Richard.Cartun at hhchealth.org Wed Jun 8 14:50:14 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Wed, 8 Jun 2016 19:50:14 +0000 Subject: [Histonet] P16 In-Reply-To: References: <003201d1c1a0$c8878db0$5996a910$@biopath.org>, Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B0D6C9A@HHCEXCHMB03.hhcsystem.org> We purchase the antibody from Ventana and run it on our Bond Max platforms. During re-optimization, we found we could dilute it 1:10 (15 minutes) following "H2" (high pH) retrieval and still get excellent results. Richard Richard W. Cartun, MS, PhD Director, Histology and the Martin M. Berman, MD Immunopathology and Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Department of Pathology and Laboratory Medicine Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax ________________________________________ From: Dessoye, Michael via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, June 08, 2016 1:14 PM To: Paula; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] P16 Ventana/Roche bought out MTM which had the patent, so they're the only vendor you can get it from. Since you're using Bond you obviously don't want it in a dispenser, but you can buy their manual staining kit and just use the antibody from that. Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Commonwealth Health Laboratory Services | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 -----Original Message----- From: Paula [mailto:plucas at biopath.org] Sent: Wednesday, June 08, 2016 12:14 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] P16 Hello, Our pathologist would like us to get the P16 antibody. We have the Bond III and we use ready to use antibodies. Leica doesn't carry this antibody and so I was hoping someone can tell me which vendor carries it and that I can order it from. Thanks in advance for your help Paula Lab manager for BioPath Medical Group in California -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From dblackburn2000 at yahoo.com Wed Jun 8 18:30:38 2016 From: dblackburn2000 at yahoo.com (daniel blackburn) Date: Wed, 8 Jun 2016 23:30:38 +0000 (UTC) Subject: [Histonet] exploding Nalgene bottles? References: <117440304.56627.1465428638598.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <117440304.56627.1465428638598.JavaMail.yahoo@mail.yahoo.com> We have always stored fixed specimens of various sizes in plastic Nalgene bottles, because they are so resistant to chemicals. However, we're recently realized that the bottles grow very brittle with age, and can "explode" into numbers shards when squeezed slightly. I think it is a result of age, not the stored fluids, since even bottles with 70% ethanol are brittle. Have others experienced this? Is it a common, well-known problem? thanks, D Blackburn, Trinity College (Hartford CT) From juan.l.bassett.ctr at mail.mil Thu Jun 9 05:12:38 2016 From: juan.l.bassett.ctr at mail.mil (Bassett, Juan L CTR USARMY MEDCOM USAMRMC (US)) Date: Thu, 9 Jun 2016 10:12:38 +0000 Subject: [Histonet] Buehler EcoMet 250 grinder Message-ID: <5BEFD2C58946394F8E2A6EC3E1F86A9B8678C2DD@UMECHPA67.easf.csd.disa.mil> If you are using a Buehler EcoMet 250 grinder , what method are you using to hold bone specimens to the pad? This is the grinder w/o the arm. Juan l. Bassett HT ASCP Histotechnician Anatomical Div. National Museum of Health and Medicine 2500 Linden Lane Silver Spring, MD 20910 Office 301-319-3359 Lab 301-319-3328 Mobile 240-505-7247 Juan.l.bassett.ctr at mail.mil From litepath2000 at yahoo.com Thu Jun 9 09:33:24 2016 From: litepath2000 at yahoo.com (NYSHisto) Date: Thu, 9 Jun 2016 14:33:24 +0000 (UTC) Subject: [Histonet] IMPORTANT: NYS Annoucment: Legislature Approves Extension of Clinical Laboratory Technology Practice Act References: <1962819548.233593.1465482804918.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1962819548.233593.1465482804918.JavaMail.yahoo@mail.yahoo.com> June 8, 2016 | Legislature Approves Extension of Clinical LaboratoryTechnology Practice Act | | | - TheState Senate and Assembly passed HANYS-supported legislation (A.8676-A,Magnarelli/S.6929-A, DeFrancisco) that extends for two years the currentlimited licensing provisions of the Clinical Laboratory TechnologyPractice Act. The bill awaits delivery to the Governor for his action. - Originallyenacted in 2005, the Clinical Laboratory Technology Practice Actestablished a limited licensing category for professionals, particularlypathologists' assistants, who did not fit into other licensingcategories. Pathologists' assistants play a key role in hospitals andlaboratories across the state??particularly in academic medicalcenters??and New York State facilities would have encountered recruitingchallenges if this limited licensing category had been allowed to expirelater this year. Authorization for this limited licensure is now set toexpire on September 1, 2018. - HANYSthanks the sponsors, Senator John DeFrancisco (R-Syracuse) and AssemblyMember William Magnarelli (D-Syracuse), for supporting this legislation.We will work with the Governor's office to urge his approval. - Arelated bill (A.10408, Harris/S.7932, Lavalle)??currently in theAssembly Rules Committee and on the Senate Calendar??would establish apermanent licensure for pathologists' assistants. HANYS continues toadvocate for its passage. From criley at dpspa.com Fri Jun 10 08:53:28 2016 From: criley at dpspa.com (Charles Riley) Date: Fri, 10 Jun 2016 09:53:28 -0400 Subject: [Histonet] Air Bubbles Message-ID: Hello everyone, Recently we have been having an issue with a majority of our slides having air bubbles under the coverslips. When they are originally coverslipped there are no bubbles and any present are pushed out and the sides are all wiped dry. We have not changed any reagent or processes in over two years. Can anyone offer suggestions as to why this might be occurring or tips to fix the issue? -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From kp5124 at outlook.com Fri Jun 10 09:15:44 2016 From: kp5124 at outlook.com (Karen Pfaff) Date: Fri, 10 Jun 2016 09:15:44 -0500 Subject: [Histonet] Air Bubbles Message-ID: There might not be enough mounting media placed on slide. So when slide is wet, there are no air bubble but after a couple of days the xylene or xylene substitute will evaporate and leave you with patchy areas. Has there been someone new coverslipping? I have been one of those people that put less mounting media on because the stage of the microscope stays cleaner, however, I discovered that I get air bubbles after it dries. Try putting a line of mounting media down the entire slide not just 2 or 3 drops. Might help.Karen PfaffLead HT Skin Cancer CenterMohs lab Sent from my U.S. Cellular? Smartphone -------- Original message -------- From: Charles Riley via Histonet Date: 06/10/2016 9:00 AM (GMT-06:00) To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Air Bubbles Hello everyone, Recently we have been having an issue with a majority of our slides having air bubbles under the coverslips. When they are originally coverslipped there are no bubbles and any present are pushed out and the sides are all wiped dry. We have not changed any reagent or processes in over two years. Can anyone offer suggestions as to why this might be occurring or tips to fix the issue? -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Fri Jun 10 11:20:49 2016 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 10 Jun 2016 12:20:49 -0400 Subject: [Histonet] ASCP Wage and Salary Survey Message-ID: <00d501d1c334$0d9af5b0$28d0e110$@earthlink.net> Hi Histonetters! I hope everybody is gearing up for a fantastic weekend. I have a quick question. Does anyone know when the next wage and salary survey will be conducted/published by ASCP? Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From barryrittman at gmail.com Fri Jun 10 18:29:53 2016 From: barryrittman at gmail.com (Barry Rittman) Date: Fri, 10 Jun 2016 18:29:53 -0500 Subject: [Histonet] Air Bubbles In-Reply-To: References: Message-ID: There might be air in the mounting media that gives rise to bubbles when drying starts to occur. One method to try is to use an ultrasonic bath to remove the air from the bottle containing the mounting media before using. We used to do this with aqueous mounting media. Barry On Fri, Jun 10, 2016 at 9:15 AM, Karen Pfaff via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > > > There might not be enough mounting media placed on slide. So when slide is > wet, there are no air bubble but after a couple of days the xylene or > xylene substitute will evaporate and leave you with patchy areas. Has there > been someone new coverslipping? I have been one of those people that put > less mounting media on because the stage of the microscope stays cleaner, > however, I discovered that I get air bubbles after it dries. Try putting a > line of mounting media down the entire slide not just 2 or 3 drops. Might > help.Karen PfaffLead HT Skin Cancer CenterMohs lab > > Sent from my U.S. Cellular? Smartphone > > -------- Original message -------- > From: Charles Riley via Histonet > Date: 06/10/2016 9:00 AM (GMT-06:00) > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Air Bubbles > > Hello everyone, > > Recently we have been having an issue with a majority of our slides having > air bubbles under the coverslips. When they are originally coverslipped > there are no bubbles and any present are pushed out and the sides are all > wiped dry. We have not changed any reagent or processes in over two years. > Can anyone offer suggestions as to why this might be occurring or tips to > fix the issue? > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From k84as at yahoo.com Sun Jun 12 14:48:13 2016 From: k84as at yahoo.com (mohamed abd el razik) Date: Sun, 12 Jun 2016 19:48:13 +0000 (UTC) Subject: [Histonet] Histoline company References: <449489329.1871493.1465760893510.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <449489329.1871493.1465760893510.JavaMail.yahoo@mail.yahoo.com> Dear All we have a limited fund for buying new automatic microtom. we have got offer from an agent for model RM3600 histoline company - Italyhave any one worked befor with such model or any recommendations please thanks From blayjorge at gmail.com Sun Jun 12 15:06:48 2016 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Sun, 12 Jun 2016 16:06:48 -0400 Subject: [Histonet] Who said Natura maxime miranda in minimis. or Natura in minima maxima. who said this? Nature is the greatest in the smallest. ? Message-ID: Dear Colleagues: Who said Natura maxime miranda in minimis. or Natura in minima maxima. who said this? Nature is the greatest in the smallest. ? Dear Colleagues: I am trying to find the source of *Natura maxime miranda in minimis*. I have also seen *Natura in minima maxima*. Nature is the greatest in the smallest. Are any of the quotes in Latin, above, written in correct Latin? If you have constructive feedback, please feel free to send me an email off the list. blayjorge at gmail.com Apologies for potential duplicate emails. With gratefulness, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From PREISZNE at mail.etsu.edu Mon Jun 13 06:12:38 2016 From: PREISZNE at mail.etsu.edu (Preiszner, Johanna) Date: Mon, 13 Jun 2016 11:12:38 +0000 Subject: [Histonet] Who said Natura maxime miranda in minimis. or Natura in minima maxima. who said this? Nature is the greatest in the smallest. ? In-Reply-To: References: Message-ID: Plini, Hist. Nat. XI. 1. The qoute below is the simplified form of the original: Quum rerum natura nusquam magis, quam in minimis, tota sit. ________________________________________ From: Jorge A. Santiago-Blay via Histonet [histonet at lists.utsouthwestern.edu] Sent: Sunday, June 12, 2016 4:06 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Who said Natura maxime miranda in minimis. or Natura in minima maxima. who said this? Nature is the greatest in the smallest. ? Who said Natura maxime miranda in minimis. or Natura in minima maxima. who said this? Nature is the greatest in the smallest. ? From tina.vanmeter at gmail.com Mon Jun 13 09:48:17 2016 From: tina.vanmeter at gmail.com (Tina Van Meter) Date: Mon, 13 Jun 2016 10:48:17 -0400 Subject: [Histonet] Ercc1 antibody Message-ID: ?Hello Histonetters, Does anyone know of a good Ercc1 antibody that works for IHC-P in mouse tissue? Thank you, Tina Tina Van Meter, HT (ASCP) Histology Core Manager Scripps Research Institute Jupiter, FL From AJohnson at aipathology.com Mon Jun 13 12:44:56 2016 From: AJohnson at aipathology.com (Amy Johnson) Date: Mon, 13 Jun 2016 17:44:56 +0000 Subject: [Histonet] Powder Picric Acid vs Liquid Picric Acid Message-ID: Our current procedure for the Brown and Brenn Gram stain uses 0.5 grams of picric acid to 400mls of acetone........is there a way to use a 1.3% Picric Acid solution in place of the powdered Picric Acid? Amylin Johnson, B.S. HTL(ASCP) Associates in Pathology Wausau Wi 54401 715-847-2130 From JMacDonald at mtsac.edu Mon Jun 13 14:00:37 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Mon, 13 Jun 2016 12:00:37 -0700 Subject: [Histonet] Powder Picric Acid vs Liquid Picric Acid In-Reply-To: References: Message-ID: 0.5 grams in 400 mL is 0.125%. Dilute your 1.3% tenfold and it will be close enough. From: Amy Johnson via Histonet To: "histonet at lists.utsouthwestern.edu" Date: 06/13/2016 10:47 AM Subject: [Histonet] Powder Picric Acid vs Liquid Picric Acid Our current procedure for the Brown and Brenn Gram stain uses 0.5 grams of picric acid to 400mls of acetone........is there a way to use a 1.3% Picric Acid solution in place of the powdered Picric Acid? Amylin Johnson, B.S. HTL(ASCP) Associates in Pathology Wausau Wi 54401 715-847-2130 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Mon Jun 13 14:58:45 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 13 Jun 2016 19:58:45 +0000 (UTC) Subject: [Histonet] Powder Picric Acid vs Liquid Picric Acid In-Reply-To: References: Message-ID: <1423722162.1980900.1465847925073.JavaMail.yahoo@mail.yahoo.com> If your 1.3% picric solution is in distilled water, there is not much you can do about it.If it is in acetone 38.4 mL contains 0.5 g of picric acid to which you can add 361.6 mL of acetone to get your desired concentration.Ren? On Monday, June 13, 2016 3:24 PM, Jennifer MacDonald via Histonet wrote: 0.5 grams in 400 mL is 0.125%.? Dilute your 1.3% tenfold and it will be close enough. From:? Amy Johnson via Histonet To:? ? "histonet at lists.utsouthwestern.edu" Date:? 06/13/2016 10:47 AM Subject:? ? ? ? [Histonet] Powder Picric Acid vs Liquid Picric Acid Our current procedure for the Brown and Brenn Gram stain uses 0.5 grams of picric acid to 400mls of acetone........is there a way to use a 1.3% Picric Acid solution in place of the powdered Picric Acid? Amylin Johnson, B.S. HTL(ASCP) Associates in Pathology Wausau Wi 54401 715-847-2130 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tawnia at ampianstaffing.com Mon Jun 13 17:24:08 2016 From: tawnia at ampianstaffing.com (Tawnia Lindsay) Date: Mon, 13 Jun 2016 22:24:08 +0000 Subject: [Histonet] travel jobs Message-ID: Hi Histonetters, Happy summer!!! Summer has brought some great travel opportunities across the country. I have jobs from the Pacific Northwest to the South and everywhere in between. Right now I have travel jobs in; Washington Nevada New Mexico Arizona Pennsylvania Oregon Missouri With new places popping up every day!!!! Let me know if you are in the market, it might not be the right time for you, but if you know someone who is looking I can give you a great referral bonus. Thanks, Tawnia Lindsay Senior Recruiter Ampian Staffing, Inc. 126 W Sego Lily Suite 110 Sandy UT 84070 O:877-229-6996 ext 2009 F:801-253-6127 email: tawnia at ampianstaffing.com Website: ampainstaffing.com From twheelock at mclean.harvard.edu Tue Jun 14 08:33:02 2016 From: twheelock at mclean.harvard.edu (Wheelock, Timothy R.) Date: Tue, 14 Jun 2016 13:33:02 +0000 Subject: [Histonet] Over-processing of brain tissue Message-ID: <69718C0B0B3C414D9F8E7214AD400CC99FBA4B88@PHSX10MB11.partners.org> Good morning everyone: I seem to be having problems with over-processing of brain tissue. I use a VIP6 processor. I start off with 1 hour each of 30%, 50%, 80%, and 95% isopropanol. Then half-hour each of three changes of absolute isopropanol. Then half hour each of three changes of xylene. Then half-hour each of 4 changes of Paraplast. I use a slow mixing cycle and no vacuum-pressure for all reagents except the paraffin. For the paraffins, I use vacuum pressure, but no mixing cycle. I rotate the paraffins during each run of tissue. I replace the dilutions of isopropanol after I have processed 500 blocks. I rotate the absolute isopropanols and xylenes after 500 blocks as well. When I embed the brain tissue, it sometimes seems a little stiff, or even a bit brittle, to one extent or another. When I trim the blocks, they seem somewhat dry. Once in a while there is even a saw-dust effect. Before I section the blocks, I have to keep them on ice for at least 3 hours before they are moist enough to cut. Even then, the first case (out of 5 cases) shows chatter in the cerebral cortex. When I examine the stained sections microscopically, even the best sections look a little "rough" or dry. Taking microscopic images can be difficult at 40x ( or even 20x) because all this roughness shows. The brain tissue looks "granular" rather than smooth, especially with a LHE stain. I have continued to reduce the times to their present values, but it still does not seem enough. I absolutely love the VIP6, but it is a much more powerful machine than the Shandon Hypercenter XP that I use to have. Perhaps I have still not fully compensated for this power. It has 4 paraffin reservoirs rather than 2 on the old Hypercenter, and the reagent reservoirs hold twice as much volume. Any ideas on how to resolve this problem? Should I reduce the times further? Should I alter the use of the mixing and/or vacuum-pressure? Thanks for any help that you can give me. Tim Tim Wheelock Assistant Director, Neuropathology Instructor of Neuroanatomy Tour Coordinator Harvard Brain Tissue Resource Center Room 203, Mailman Research Center McLean Hospital, Belmont, MA 02478 Phone: 617-855-3592 Cell: 857-234-9311 Fax: 617-855-3199 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From amurvosh at advancederm.net Tue Jun 14 09:00:20 2016 From: amurvosh at advancederm.net (Anne Murvosh) Date: Tue, 14 Jun 2016 14:00:20 +0000 Subject: [Histonet] Spill kits Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7B0D715@Exchange.Advancederm.net> Do spill kits need to be out where anyone can see them, or can I put it in a cupboard with a sign on the door? I need to move mine and there's very little room. Thanks Anne From allyse124 at gmail.com Tue Jun 14 09:48:00 2016 From: allyse124 at gmail.com (Allyse Mazzarelli) Date: Tue, 14 Jun 2016 10:48:00 -0400 Subject: [Histonet] Help with liver/heart tissues Message-ID: Good afternoon, I am having a few issues getting nice morphology from both liver and heart tissues. I currently work only with CNS tissues (brain, spinal cord, DRG, etc.) but recently, our research team have become interested in immunostaining some peripheral tissues, including the heart and liver. All tissue [mouse] is perfusion fixed with 4% PFA and then post-fixed in fresh 4% PFA for at least 24 - 48 hours. After fixation, the tissue is cryoprotected in 30% sucrose solution for 48 hours. Once cryoprotected, I then section the tissue at various thicknesses depending on the assay I'm running. Previously I sectioned some heart and liver samples (sectioned both at 20um and at 8um) and ran a few H&Es. Unfortunately, the tissue was so damaged and under-fixed that we scrapped the blocks. This time around, the mouse liver tissue was carefully dissected prior to post-fixation into four quadrants to allow for better PFA permealization. Additionally, we post fixed in 4% PFA for 3 days instead of 2, and the tissue was cryoprotected for 2 days. To my surprise, when I sectioned this tissue on the cryostat, I still noticed severe artifacts. It is very difficult to see nice morphology, and there appears that there was an issue with fixation (in the liver the nucleus is flattened and the sinusoids are not clear, etc. the nuclei in the heart are also more flat and the muscle fibers have separated from one another). The staining was not as bad as the first tissue I had sectioned, but I was still unable to get a nice H&E stain depicting clear nuclear/cytoplasm. These artifacts appeared at both 8um and 20um. Does anyone happen to have nice fixation/H&E staining protocols for both liver and heart? I'd be happy to give an in-depth description of the protocol(s) I'm currently using. Additionally, is there anything that appears I'm doing wrong in terms of perfusion/fixation? Thanks so much! Allyse From rjbuesa at yahoo.com Tue Jun 14 11:06:59 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 14 Jun 2016 16:06:59 +0000 (UTC) Subject: [Histonet] Over-processing of brain tissue In-Reply-To: <69718C0B0B3C414D9F8E7214AD400CC99FBA4B88@PHSX10MB11.partners.org> References: <69718C0B0B3C414D9F8E7214AD400CC99FBA4B88@PHSX10MB11.partners.org> Message-ID: <804801747.2375921.1465920419941.JavaMail.yahoo@mail.yahoo.com> It seems to me you are processing too much unless the slices are 3mm thick or more.I suggest you to cut the dehydration to 45 minutes (the sequence seems OK)Reduce the pure 2-propanol to just 2 changes (30 min is OK)Add 1 change of a mixture 1:1 of 2-propanol and xylene + 2 xylene stepsthen to paraffinUse vacuum and mixing/agitation in all steps (it will help)If you want really to simplify, use 2-propanol in all steps instead of ethanol and eliminate the xylene. You can go from pure 2-propanol ? 1:1 mixture of 2-propanol with paraffin ? 3 paraffin changes.You will eliminate xylene and obtain very good results.Contact me if you need some publications on this procedure.Ren? On Tuesday, June 14, 2016 9:53 AM, "Wheelock, Timothy R. via Histonet" wrote: Good morning everyone: I seem to be having problems with over-processing of brain tissue. I use a VIP6 processor. I start off with 1 hour each of 30%, 50%, 80%, and 95% isopropanol. Then half-hour each of three changes of absolute isopropanol. Then half hour each of three changes of xylene. Then half-hour each of 4 changes of Paraplast. I use a slow mixing cycle and no vacuum-pressure for all reagents except the paraffin. For the paraffins, I use vacuum pressure, but no mixing cycle. I rotate the paraffins during each run of tissue. I replace the dilutions of isopropanol after I have processed 500 blocks. I rotate the absolute isopropanols and xylenes after 500 blocks as well. When I embed the brain tissue, it sometimes seems a little stiff, or even a bit brittle, to one extent or another. When I trim the blocks, they seem somewhat dry. Once in a while there is even a saw-dust effect. Before I section the blocks, I have to keep them on ice for at least 3 hours before they are moist enough to cut. Even then, the first case (out of 5 cases) shows chatter in the cerebral cortex. When I examine the stained sections microscopically, even the best sections look a little "rough" or dry. Taking microscopic images can be difficult at 40x ( or even 20x) because all this roughness shows. The brain tissue looks "granular" rather than smooth, especially with a LHE stain. I have continued to reduce the times to their present values, but it still does not seem enough. I absolutely love the VIP6, but it is a much more powerful machine than the Shandon Hypercenter XP that I use to have. Perhaps I have still not fully compensated for this power. It has 4 paraffin reservoirs rather than 2 on the old Hypercenter, and the reagent reservoirs hold twice as much volume. Any ideas on how to resolve this problem? Should I reduce the times further? Should I alter the use of the mixing and/or vacuum-pressure? Thanks for any help that you can give me. Tim Tim Wheelock Assistant Director, Neuropathology Instructor of Neuroanatomy Tour Coordinator Harvard Brain Tissue Resource Center Room 203, Mailman Research Center McLean Hospital, Belmont, MA 02478 Phone: 617-855-3592 Cell:? ? 857-234-9311 Fax:? ? 617-855-3199 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Tue Jun 14 11:08:46 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 14 Jun 2016 16:08:46 +0000 (UTC) Subject: [Histonet] Spill kits In-Reply-To: <22BDD9AABC13E24E95D1CF064B75C4B7B0D715@Exchange.Advancederm.net> References: <22BDD9AABC13E24E95D1CF064B75C4B7B0D715@Exchange.Advancederm.net> Message-ID: <367834512.2408444.1465920526932.JavaMail.yahoo@mail.yahoo.com> What you need to do is to communicate to everybody where the kits are, and place them where it is more convenient for you. Once everybody knows the location, a good sign is always a plus.Ren? On Tuesday, June 14, 2016 10:15 AM, Anne Murvosh via Histonet wrote: Do spill kits need to be out where anyone can see them, or can I put it in a cupboard with a sign on the door?? I need to move mine and there's very little room.? Thanks Anne _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff at uropartners.com Tue Jun 14 11:11:43 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 14 Jun 2016 16:11:43 +0000 Subject: [Histonet] Spill kits In-Reply-To: <22BDD9AABC13E24E95D1CF064B75C4B7B0D715@Exchange.Advancederm.net> References: <22BDD9AABC13E24E95D1CF064B75C4B7B0D715@Exchange.Advancederm.net> Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10E137A8@COLOEXCH01.uropartners.local> We keep our spill kits in unobstructed cabinets with bright yellow signage on the wall above. We have no problem getting to them as needed, and have never had an issue in many, many CAP inspections. ------------------------------ ------------------------------ Latest post: http://www.chicagonow.com/downsize-maybe/2016/06/fathers-day-and-lee-the-dad-i-hardly-knew/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: Anne Murvosh via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 14, 2016 9:00 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Spill kits Do spill kits need to be out where anyone can see them, or can I put it in a cupboard with a sign on the door? I need to move mine and there's very little room. Thanks Anne _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JRobinson at pathology-associates.com Tue Jun 14 11:27:12 2016 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Tue, 14 Jun 2016 16:27:12 +0000 Subject: [Histonet] PAX 2 Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C90A24E91@PAEXCH1.PathologyAssociates.local> Good Morning- can anyone give me a recommendation for a predilute PAX2 antibody? The one I was using has been discontinued and the replacement product does not seem to perform very well. I will be using it on the Bond III. Thanks in advance! Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From Catherine.L.Scott at uth.tmc.edu Tue Jun 14 11:46:14 2016 From: Catherine.L.Scott at uth.tmc.edu (Scott, Catherine L) Date: Tue, 14 Jun 2016 16:46:14 +0000 Subject: [Histonet] (no subject) Message-ID: <4571339ED5505F4F8AD51D2280C4C0EA162E80A4@UTHMAIL2.uthouston.edu> UT Path located in the medical center (Houston) is currently looking for a Medical Technologist that is able to work on an as needed basis, hours are negotiable. Duties include, but not limited to performing molecular testing using the Hologic Panther system, this could lead to a full time position for the right candidate. In addition, to the MT position, we also have available a full time HTL position performing immunohistochemical staining utilizing the Dako system. We offer completive salaries and great benefits. Please contact me at 713-500-5401 or Catherine.L.Scott @uth.tmc.edu. From rachel at gbi-inc.com Tue Jun 14 13:09:16 2016 From: rachel at gbi-inc.com (Rachel M Gonzalez) Date: Tue, 14 Jun 2016 14:09:16 -0400 Subject: [Histonet] Tissue controls Message-ID: Hi Everyone, I am trying to help out a coworker with a survey on types of tissue and controls needed for both clinical and research labs. She has a short deadline by next Monday. She if in charge of the OriGene Technologies tissue division. If you could take a few minutes to fill in the survey for a really nice coworker (Julie) that would be great. https://www.surveymonkey.com/r/QMGWKHZ Thanks for any help. Rachel From gmartin at marshallmedical.org Tue Jun 14 16:10:55 2016 From: gmartin at marshallmedical.org (Martin, Gary) Date: Tue, 14 Jun 2016 14:10:55 -0700 Subject: [Histonet] Cytology specimens Message-ID: <6ED9D4252F278841A0593D3D788AF24C366E5CF8@mailsvr.MARSHMED.local> I would like to poll the Histo group concerning cytology specimens. The discussion has come up about when to discard a fluid after processing, and fluids that have no orders. Also, because we are a contracted service for the hospital, and are not connected to their LIS. Cytology's that are ordered do produce a requisition that sometimes does not follow the specimen, which leads to missed processing. How are others handling these two situations. Thanks From sk at personifysearch.com Tue Jun 14 16:40:43 2016 From: sk at personifysearch.com (Sarah Kelly) Date: Tue, 14 Jun 2016 17:40:43 -0400 Subject: [Histonet] Several New Histology Career Opportunities!! Message-ID: Hello Histonet, Our exclusively retained client has opened several new opportunities across the U.S., and I wanted to reach out regarding a few specifics and see if anyone would be interested in learning more. Our client is a global leader in cancer diagnostics and we are looking for individuals with a strong histology background, IHC, and troubleshooting experience. The company is part of a $20 billion organization and growing. These positions are full-time, direct-hire roles with competitive salaries and full benefits. If you or anyone you know may be interested in learning more about these opportunities, please contact me directly at *sk at personifysearch.com *. Thank you! Sarah Sarah Kelly Personify, Talent Management Executive *sk at personifysearch.com * (800) 875-6188, ext.153 From relia1 at earthlink.net Wed Jun 15 10:21:19 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 15 Jun 2016 11:21:19 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin. The Catbird Seat 6/15/2016 Message-ID: <00ed01d1c719$920e8530$b62b8f90$@earthlink.net> Hi Histonetters! How are you? I hope you are having a great week! Have you ever heard the saying "In the Catbird Seat"? It means you are calling the shots. That's what's happening in histology now! If you are an ASCP certified histotech with at least 2 years of experience YOU are in the CATBIRD SEAT! Things have come full cycle and you are in GREAT DEMAND. There are many more histology jobs than histotechs. There are sign on bonuses, leading edge training opportunities and amazing perks waiting for you!! If you are considering a job change: *Because you want to relocate *Because you feel unchallenged *Because you want more money, a better shift, nicer benefits Whatever the reason. **STRIKE WHILE THE IRON'S HOT!! Shoot me a quick email and let me know what you would like to do and where you would like to go and when (no pressure the timing is up to YOU!) I will keep you posted on opportunities that match your interests. **REMEMBER IT NEVER HURTS TO LOOK!! I am including a list of my current opportunities in case one might strike your fancy. Please feel free to pass the info along to your friends and coworkers. If I place someone you refer to me you will earn a referral reward! RELIA'S Current Histology Opportunities Austin, TX - Histology Tech - Early morning shift, generous shift diff! Virginia Beach, VA - Histotechnician - 15K Sign On Bonus! Fayetteville, AR - Lead Dermpath Histotech - Run your own Lab! Flagstaff, AZ - Histotech IHC/ISH - Days Nashville, TN - Histology Manager Milwaukee, WI - Histotechnician - Days Roswell, NM - Assistant Supervisor -Days Nashville, TN - Histotech early morning shift Lafayette, LA - Histotechnician Early Morning Shift Tyler, TX - Senior Histotechnician - Days Baton Rouge, LA - Histology Tech - nights All of my clients offer excellent compensation, benefits and some offer relocation assistance and or sign on bonuses. All of these jobs are full time & permanent & most of them are RELIA Exclusives!!! I can be reached ASAP via email at relia1 at earthlink.net or toll free at the office at 866-607-3542 or on my cell at 407-353-5070 call or text! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From doolee at shands.ufl.edu Wed Jun 15 12:05:11 2016 From: doolee at shands.ufl.edu (Dooley, Elaine) Date: Wed, 15 Jun 2016 17:05:11 +0000 Subject: [Histonet] PLA2R1 Message-ID: <6193C53146742E4586DD4838F47F79AE0EA61FB1@MSXMB02.Shands.local> Dear Histonetters, Does anyone make an anti-human PLA2R1 antibody directly conjugated to fluorescein? I am having background problems trying to use an two step indirect staining method on frozen kidney sections. Elaine Dooley Shands Teaching Hospital Gainesville FL 352-265-0111 ext 72117 From tbraud at holyredeemer.com Wed Jun 15 13:15:09 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 15 Jun 2016 18:15:09 +0000 Subject: [Histonet] Cytology specimens Message-ID: <48E053DDF6CE074DB6A7414BA05403F8079CA6@HRHEX02-HOS.holyredeemer.local> We used to have problems with fluids/orders, but what we put in place seems to be working. 1. All fluids are received by central processing. If a fluid has no orders, then central processing calls to have the orders put in. Cytology does not accept fluids without orders. We also encourage (though not required) the physicians to fill out a "Fluid Checklist" to accompany the specimen. Central processing insures that the orders were placed correctly according to that sheet, and rectifies any problems or conflicts. 2. Fluids are aliquoted in central processing for all tests ordered with the exception of a specimen to be shared by Micro and Cytology, then it is send to Micro with a "Shared" sticker and the AP order stickers accompanying the specimen. Micro takes what they need and passes the specimen on to us. 3. In cases of large volume fluids, central processing aliquots the fluid for all the departments and then sends the large balance for any cytology orders 3. With the exception of a urine, which receives a Thin Prep only, all fluids are prepared with a Thin Prep and if possible, a cell block. 4. With the exception of a urine, if the specimen is large, after the preps our techs will pour off a 50 ml aliquot into a sterile tube, label and refrigerate for 2 weeks. 5. The balance of bulk fluids are discarded after processing. I hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Today's Topics: 2. Cytology specimens (Martin, Gary) Message: 2 Date: Tue, 14 Jun 2016 14:10:55 -0700 From: "Martin, Gary" Subject: [Histonet] Cytology specimens I would like to poll the Histo group concerning cytology specimens. The discussion has come up about when to discard a fluid after processing, and fluids that have no orders. Also, because we are a contracted service for the hospital, and are not connected to their LIS. Cytology's that are ordered do produce a requisition that sometimes does not follow the specimen, which leads to missed processing. How are others handling these two situations. Thanks **************** From sprice2003 at gmail.com Wed Jun 15 13:26:03 2016 From: sprice2003 at gmail.com (Sally Price) Date: Wed, 15 Jun 2016 14:26:03 -0400 Subject: [Histonet] Tissue Controls (Deceptive) Message-ID: Not cool Rachel. ?I took the survey assuming that it was intended and might help a colleage, but it's nothing but a marketing ploy, which are strongly discouraged on this forum. -------- Original message -------- From histonet-request at lists.utsouthwestern.edu Date: 06/15/2016 1:00 PM (GMT-05:00) To histonet at lists.utsouthwestern.edu Subject Histonet Digest, Vol 151, Issue 14 --------------------------------------------------------------------- Message: 1 Date:?Tue, 14 Jun 2016 14:09:16?-0400 From: Rachel M Gonzalez To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Tissue controls Message-ID: Content-Type: text/plain; charset=UTF-8 Hi Everyone, I am trying to help out a coworker with a survey on types of tissue and controls needed for both clinical and research labs. She has a short deadline by next Monday. She if in charge of the OriGene Technologies tissue division.? If you could take a few minutes to fill in the survey for a really nice coworker (Julie) that would be great. https://www.surveymonkey.com/r/QMGWKHZ Thanks for any help. Rachel From jaylundgren at gmail.com Wed Jun 15 14:52:15 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 15 Jun 2016 12:52:15 -0700 Subject: [Histonet] Tissue Controls (Deceptive) In-Reply-To: References: Message-ID: Get out the pitchforks and torches! On Wed, Jun 15, 2016 at 11:26 AM, Sally Price via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Not cool Rachel. I took the survey assuming that it was intended and > might help a colleage, but it's nothing but a marketing ploy, which are > strongly discouraged on this forum. > > -------- Original message -------- > From histonet-request at lists.utsouthwestern.edu > Date: 06/15/2016 1:00 PM (GMT-05:00) > To histonet at lists.utsouthwestern.edu > Subject Histonet Digest, Vol 151, Issue 14 > > --------------------------------------------------------------------- > > Message: 1 > Date: Tue, 14 Jun 2016 14:09:16 -0400 > From: Rachel M Gonzalez > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Tissue controls > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Hi Everyone, > > > I am trying to help out a coworker with a survey on types of tissue and > controls needed for both clinical and research labs. She has a short > deadline by next Monday. > > She if in charge of the OriGene Technologies tissue division. If you could > take a few minutes to fill in the survey for a really nice coworker (Julie) > that would be great. > > https://www.surveymonkey.com/r/QMGWKHZ > > > Thanks for any help. > > Rachel > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jford at cytomx.com Wed Jun 15 15:16:14 2016 From: jford at cytomx.com (Judi Ford) Date: Wed, 15 Jun 2016 20:16:14 +0000 Subject: [Histonet] Staining stomach cells Message-ID: Hi everyone, I'm trying to differentiate parietal vs chief vs endocrine cells in the stomach. Any ideas on stains or antibodies I could use. Thanks, judi From jaylundgren at gmail.com Wed Jun 15 17:56:19 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 15 Jun 2016 15:56:19 -0700 Subject: [Histonet] Staining stomach cells In-Reply-To: References: Message-ID: On H&E, parietal cells are eosinophillic , due to abundant mitochondria. Chief cells have basophillic cytoplasm on H&E, due to abundant rough endoplasmic reticulum. Other than that, I'd think you'd want to use IHC. Is here an antibody for intrinsic factor? Parietal cells secrete intrinsic factor, chief cells do not. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) P.S. Abcam makes an anti-intrinsic factor Ab for IHC. Make sure to spell my name right on the paper. ;P On Wed, Jun 15, 2016 at 1:16 PM, Judi Ford via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi everyone, > I'm trying to differentiate parietal vs chief vs endocrine cells in the > stomach. Any ideas on stains or antibodies I could use. > Thanks, > judi > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From W.E.J.Hoekert at olvg.nl Thu Jun 16 01:45:18 2016 From: W.E.J.Hoekert at olvg.nl (Hoekert, Willem) Date: Thu, 16 Jun 2016 06:45:18 +0000 Subject: [Histonet] Staining stomach cells In-Reply-To: References: , Message-ID: Maybe use Gastrin for the endocrine cells? Willem ________________________________________ Van: Jay Lundgren via Histonet [histonet at lists.utsouthwestern.edu] Verzonden: donderdag 16 juni 2016 0:56 Aan: Judi Ford CC: histonet at lists.utsouthwestern.edu Onderwerp: Re: [Histonet] Staining stomach cells On H&E, parietal cells are eosinophillic , due to abundant mitochondria. Chief cells have basophillic cytoplasm on H&E, due to abundant rough endoplasmic reticulum. Other than that, I'd think you'd want to use IHC. Is here an antibody for intrinsic factor? Parietal cells secrete intrinsic factor, chief cells do not. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) P.S. Abcam makes an anti-intrinsic factor Ab for IHC. Make sure to spell my name right on the paper. ;P On Wed, Jun 15, 2016 at 1:16 PM, Judi Ford via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi everyone, > I'm trying to differentiate parietal vs chief vs endocrine cells in the > stomach. Any ideas on stains or antibodies I could use. > Thanks, > judi > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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From rachel at gbi-inc.com Thu Jun 16 09:38:30 2016 From: rachel at gbi-inc.com (Rachel M Gonzalez) Date: Thu, 16 Jun 2016 10:38:30 -0400 Subject: [Histonet] Tissue Controls (Deceptive) In-Reply-To: References: Message-ID: Hi I am really sorry to everyone. You have always been so helpful to me I just wanted to help a colleague. Sorry, I am a scientist and did not realize the implication as unfair. I will not do that again. Rachel On Wed, Jun 15, 2016 at 3:52 PM, Jay Lundgren via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Get out the pitchforks and torches! > > On Wed, Jun 15, 2016 at 11:26 AM, Sally Price via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Not cool Rachel. I took the survey assuming that it was intended and > > might help a colleage, but it's nothing but a marketing ploy, which are > > strongly discouraged on this forum. > > > > -------- Original message -------- > > From histonet-request at lists.utsouthwestern.edu > > Date: 06/15/2016 1:00 PM (GMT-05:00) > > To histonet at lists.utsouthwestern.edu > > Subject Histonet Digest, Vol 151, Issue 14 > > > > --------------------------------------------------------------------- > > > > Message: 1 > > Date: Tue, 14 Jun 2016 14:09:16 -0400 > > From: Rachel M Gonzalez > > To: "histonet at lists.utsouthwestern.edu" > > > > Subject: [Histonet] Tissue controls > > Message-ID: > > > > Content-Type: text/plain; charset=UTF-8 > > > > Hi Everyone, > > > > > > I am trying to help out a coworker with a survey on types of tissue and > > controls needed for both clinical and research labs. She has a short > > deadline by next Monday. > > > > She if in charge of the OriGene Technologies tissue division. If you > could > > take a few minutes to fill in the survey for a really nice coworker > (Julie) > > that would be great. > > > > https://www.surveymonkey.com/r/QMGWKHZ > > > > > > Thanks for any help. > > > > Rachel > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From CDavis at che-east.org Thu Jun 16 11:48:37 2016 From: CDavis at che-east.org (Cassie P. Davis) Date: Thu, 16 Jun 2016 16:48:37 +0000 Subject: [Histonet] Histology Medical Waste Message-ID: <5C815EADE724D14AA0CC8F037C4185F079B322C5@SB01MSTMBX13.sb.trinity-health.org> In May, Curt sent this out to Histoland: "Message: 2 Date: Wed, 11 May 2016 20:18:52 +0000 From: Curt To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] embedding and microtomy "medical waste" Message-ID: <9C8F910F72893643B3C3793C3D67132B67CBAFD6 at PATHOLOGYSERVER.pathologyarts.local> Content-Type: text/plain; charset="us-ascii" So here's a good one for you all... we had the county health department come through the lab and ding us on medical waste... specifically the plastic disposable lids at embedding, the lens paper used for wrapping specimens such as ECC and EMB. Then they got us on the Kim Wipes used to clean the water bath at microtomy... those papers have human tissue on them so they need to be treated as medical waste... NOW we have to have red cans next to all microtomes and embedding stations. The obvious issue outside of cost and logic is that these medical waste cans all seem to have self closing lids which really interferes with the rhythm and pace of work when one needs to reach over with a food to open the lid after every block is embedded and when they water bath is cleaned after every block... Simple question(s): 1)does anyone else have to do such things to contain the waste, 2) does anyone know of a source for medical waste cans that do not have these frustrating self closing lids... if we could simple remove the lid and replace it when done then we could deal with it, the cost is one thing but slowing down work flow is a problem. And just for a little more humor, they actually wanted me to contain and dispose of the water runoff from our two automated slide stainers, we run about 2200 slides a night... that would be many gallons of waste water every night and would not be within the budget.... We in turn ran a fish kill test which demonstrated that the water runoff which contain little Hematoxylin, bluing and clarifier do not pose any significant threat to the environment, not even in California.... Bottom line to all this, I need some red trash cans with removable lids, if they're still out there somewhere.... Anywhere..... Thanks for your input, Curt Ps, I didn't proff read thie smail... if something is not spelt correctly, don't hold it against me...." I forwarded this to my supervisor, hoping to be proactive. She forwarded it to the person who is in charge of safety at our facitlity. He would like to know which states require the block and slide to be put in biohazard containers before we invest in the containers. Histofolks, please help another Histonetter out and let me know where you are and if you are required to put your shavings, block and or slides in biohazard medical waste. Thanks! Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From halsteaj at ohsu.edu Thu Jun 16 12:14:21 2016 From: halsteaj at ohsu.edu (Jeff Halstead) Date: Thu, 16 Jun 2016 17:14:21 +0000 Subject: [Histonet] warthin-starry stain Message-ID: <398DA4E9BECCE44288904391038A80DE45C14844@EXMB04.ohsu.edu> Hello histonet---I am a tech in western Oregon, and I have been experiencing strange problems with the afore mentioned stain. When the stain is run a very strange grey precipitate deposits upon the slides. The deposition is not uniform across the slides and varies from slide to slide within the run. I use bleach washed slides and all glassware is also bleach washed. The slides I am using are non charged with only frosted top. The last two runs I used reagents from just opened bottles. No meatal contacts the slides or the glassware used to assimilate the reagents. As I said---what gives. To say I am perplexed is understating my mood. I have even tried washing the slides in the hot water bath to no improvement. My path has a list of cases to run and is a bit anxious. Please help. Anyone with any ideas ? one step I did not mention-I bleach wash the slides-wash in di water-soak in 100% ethanol-then air dry and cover. Also thought I might mention I de-wax the pookers from 30 minutes to 45 minutes. Thanx for the time jeff From JMacDonald at mtsac.edu Thu Jun 16 13:06:29 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Thu, 16 Jun 2016 11:06:29 -0700 Subject: [Histonet] warthin-starry stain In-Reply-To: <398DA4E9BECCE44288904391038A80DE45C14844@EXMB04.ohsu.edu> References: <398DA4E9BECCE44288904391038A80DE45C14844@EXMB04.ohsu.edu> Message-ID: are you doing a microwave method or traditional? From: Jeff Halstead via Histonet To: "histonet at lists.utsouthwestern.edu" Date: 06/16/2016 10:17 AM Subject: [Histonet] warthin-starry stain Hello histonet---I am a tech in western Oregon, and I have been experiencing strange problems with the afore mentioned stain. When the stain is run a very strange grey precipitate deposits upon the slides. The deposition is not uniform across the slides and varies from slide to slide within the run. I use bleach washed slides and all glassware is also bleach washed. The slides I am using are non charged with only frosted top. The last two runs I used reagents from just opened bottles. No meatal contacts the slides or the glassware used to assimilate the reagents. As I said---what gives. To say I am perplexed is understating my mood. I have even tried washing the slides in the hot water bath to no improvement. My path has a list of cases to run and is a bit anxious. Please help. Anyone with any ideas ? one step I did not mention-I bleach wash the slides-wash in di water-soak in 100% ethanol-then air dry and cover. Also thought I might mention I de-wax the pookers from 30 minut es to 45 minutes. Thanx for the time jeff _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From halsteaj at ohsu.edu Thu Jun 16 13:12:56 2016 From: halsteaj at ohsu.edu (Jeff Halstead) Date: Thu, 16 Jun 2016 18:12:56 +0000 Subject: [Histonet] warthin-starry Message-ID: <398DA4E9BECCE44288904391038A80DE45C1588A@EXMB04.ohsu.edu> Hi all thanx for the tips-I am not really allowed to use acid washes-don't have access to a reliable microwave-the stain is done by hand with me assimilating the different reagents-or a mastertech kit. The idea of the bleach causing problems is interesting-before I was told not to use the strong acid washes-now that I ponder---I do have a bit of the old solution on hand might try one run with-nobody please mention this-with glassware washed the old fashioned way. Other than the bleach-am at loss as to cause of the precipitate. By the by do not use any type of gelatin or adhesive in the water bath. From criley at dpspa.com Thu Jun 16 13:17:06 2016 From: criley at dpspa.com (Charles Riley) Date: Thu, 16 Jun 2016 14:17:06 -0400 Subject: [Histonet] Poor Staining Message-ID: Can over processing small biopsies lead to poor chromatin staining? Also can this cause an issue with the eosin staining ( for example only getting to shades of pink instead of three)? Please give me any feedback you can -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From JMacDonald at mtsac.edu Thu Jun 16 13:51:05 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Thu, 16 Jun 2016 11:51:05 -0700 Subject: [Histonet] Poor Staining In-Reply-To: References: Message-ID: Heat can cause poor chromatin staining. Is the two shades of eosin only on the biopsies, or all tissues? From: Charles Riley via Histonet To: histonet at lists.utsouthwestern.edu Date: 06/16/2016 11:19 AM Subject: [Histonet] Poor Staining Can over processing small biopsies lead to poor chromatin staining? Also can this cause an issue with the eosin staining ( for example only getting to shades of pink instead of three)? Please give me any feedback you can -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DSiena at statlab.com Thu Jun 16 14:29:03 2016 From: DSiena at statlab.com (Debra Siena) Date: Thu, 16 Jun 2016 19:29:03 +0000 Subject: [Histonet] Poor Staining In-Reply-To: References: Message-ID: Hi Charles Getting three shades of pink depends a kit on your dehydration steps after eosin. Eosin is more soluble in water than alcohol so you have to watch how much water is being absorbed from the atmosphere and change/rotate alcohols when you see the last absolute turning pink, also if you have diluted alcohol after eosin you may wish to cut down or remove. I don't use a diluted alcohol at all just straight absolute alcohol. I recommend 3-4 changes of absolute alcohol for 1 minute each. For nuclear detail that could be caused by over processing and removing the bound water, If this is an occasional occurrence but if happening in all tissues large and small, It may also be that your hematoxylin and acid rinse need some type of adjustment. If I can help you offline please let me know. Thanks Debbie Siena, HT(ASCP)QIHC Sent from my iPhone > On Jun 16, 2016, at 1:20 PM, Charles Riley via Histonet wrote: > > Can over processing small biopsies lead to poor chromatin staining? Also > can this cause an issue with the eosin staining ( for example only getting > to shades of pink instead of three)? > > Please give me any feedback you can > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rachel at gbi-inc.com Thu Jun 16 16:15:41 2016 From: rachel at gbi-inc.com (Rachel M Gonzalez) Date: Thu, 16 Jun 2016 17:15:41 -0400 Subject: [Histonet] Tissue controls error and appology Message-ID: Hi Everyone, I want to send a sincere apology to the histonet group. I realized now that I was wrong to send out this survey even if it was with the good intention to help my friend. I am sorry, I honestly did not realize the implication and how unfair this was. In the past, you have helped me to learn to cut tissue suggested great antibodies, and given great advice on some practical applications. I feel that this is an IHC community that I can turn to for help but realized now although I was trying to help a friend in this format I was wrong. I will not make out that mistake again. I hope you will accept my apology. Sincerely, Rachel From tkngflght at yahoo.com Fri Jun 17 09:52:04 2016 From: tkngflght at yahoo.com (Cheryl) Date: Fri, 17 Jun 2016 14:52:04 +0000 (UTC) Subject: [Histonet] Two open positions - References: <1147290680.5271701.1466175124557.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1147290680.5271701.1466175124557.JavaMail.yahoo@mail.yahoo.com> Two open positions-- ????The first one is a solo lab in a really amazing small community - run your own lab! Great equipment, brand new & set up by someone I trust-- ????Next -a very well run lab needs a supervisor. The manager is a good guy - known him for years. It's a place I'd work-- Curious? Hit me and for more details!! ?Cheryl Kerry, HT(ASCP) Full Staff Inc. ? admin at fullstaff.org?800.756.3309 Phone & Fax (PLEASE leave a message - traveling for a few days) https://www.facebook.com/TheHistologyCompany/ From kjgada at gmail.com Fri Jun 17 10:11:45 2016 From: kjgada at gmail.com (Komal Gada) Date: Fri, 17 Jun 2016 11:11:45 -0400 Subject: [Histonet] Two open positions - In-Reply-To: <1147290680.5271701.1466175124557.JavaMail.yahoo@mail.yahoo.com> References: <1147290680.5271701.1466175124557.JavaMail.yahoo.ref@mail.yahoo.com> <1147290680.5271701.1466175124557.JavaMail.yahoo@mail.yahoo.com> Message-ID: Hello, Where is this lab located? Thanks, Komal On Jun 17, 2016 11:09 AM, "Cheryl via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > Two open positions-- > The first one is a solo lab in a really amazing small community - run > your own lab! Great equipment, brand new & set up by someone I trust-- > Next -a very well run lab needs a supervisor. The manager is a good > guy - known him for years. It's a place I'd work-- > Curious? Hit me and for more details!! Cheryl Kerry, HT(ASCP) > Full Staff Inc. > admin at fullstaff.org 800.756.3309 Phone & Fax (PLEASE leave a message - > traveling for a few days) > > https://www.facebook.com/TheHistologyCompany/ > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rmhickey88 at gmail.com Fri Jun 17 10:12:06 2016 From: rmhickey88 at gmail.com (Ryan Michael Hickey) Date: Fri, 17 Jun 2016 10:12:06 -0500 Subject: [Histonet] Open Storage of Retained Specimen Containers Message-ID: Hi Histonet, Is storage of sealed, formalin-containing, retained specimens (post-gross) acceptable in open, metal shelving units within a designated area, or do these specimens need to be in a metal cabinet or in a closed, separated room under special conditions (e.g., negative pressure)? Thanks for any insights you may be able to provide! Regards, *Ryan Hickey, MS, HTL(ASCP)CMQIHCCM* Pathologists? Assistant *UTHealth* | The University of Texas Health Science Center at Houston | Medical School *Houston?s Health University * From taylor at prometheushealthcare.com Fri Jun 17 12:26:53 2016 From: taylor at prometheushealthcare.com (Taylor Rinaldi) Date: Fri, 17 Jun 2016 13:26:53 -0400 Subject: [Histonet] **Histology Job Opportunities** Message-ID: <025c01d1c8bd$725aea30$5710be90$@prometheushealthcare.com> Happy Friday everyone! My name is Taylor Rinaldi, Recruiting Manager at Prometheus healthcare. My team and I specialize specifically in laboratory recruiting nationwide for different hospitals and reference laboratories. We are currently recruiting for multiple histology bench and supervisory opportunities throughout the United States. All the opportunities are fulltime, and permanent. ASCP certification is preferred but not required for all. If you or any of your colleagues have been considering a new position in the Histology field, please don't hesitate to reach out to me directly for immediate referral and submittal to some of the top hospitals and labs nationwide. Current states: California New York Georgia Florida New Hampshire Arkansas Illinois Texas Thank you all in advance, Taylor Rinaldi Recruiting Manager Prometheus Healthcare Office 866-857-1434 Taylor at prometheushealthcare.com From alamberth at lji.org Fri Jun 17 13:16:03 2016 From: alamberth at lji.org (Angela Lamberth) Date: Fri, 17 Jun 2016 11:16:03 -0700 Subject: [Histonet] looking for service contract providers (Southern California) Message-ID: Greetings Histonet! Can anyone recommend a service contract provider for a VIP E-150 processor and a Microm HM505E cryostat? We are located in San Diego County. Thanks! -- Angela Lamberth Histology Technician II Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 From gu.lang at gmx.at Sat Jun 18 01:27:26 2016 From: gu.lang at gmx.at (Gudrun Lang) Date: Sat, 18 Jun 2016 08:27:26 +0200 Subject: [Histonet] Staining stomach cells In-Reply-To: References: Message-ID: <000b01d1c92a$8313d720$893b8560$@gmx.at> Did you look at the mucin-antibodies? Pepsinogen-IHC should be positiv in chief-cells. Gastrin-IHC should be positiv in parietal-cells. Chromogranin A should be positiv in endocrine cells. just my ideas. please confirm with your own research. Gudrun -----Urspr?ngliche Nachricht----- Von: Judi Ford via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 15. Juni 2016 22:16 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Staining stomach cells Hi everyone, I'm trying to differentiate parietal vs chief vs endocrine cells in the stomach. Any ideas on stains or antibodies I could use. Thanks, judi _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly at merck.com Tue Jun 21 08:59:58 2016 From: brett_connolly at merck.com (Connolly, Brett M) Date: Tue, 21 Jun 2016 09:59:58 -0400 Subject: [Histonet] CD3 antibody for FFPE mouse tissue Message-ID: Hi all, Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections. I have some old Histonet messages referring to a Neomarkers RB-360 ab which I found through Thermo, but I would like to hear about more recent experiences/recommendations.... Thanks as always, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From liz at premierlab.com Tue Jun 21 10:19:49 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Tue, 21 Jun 2016 09:19:49 -0600 Subject: [Histonet] CD3 antibody for FFPE mouse tissue In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02BEC711583D@SBS2K8.premierlab.local> Brett Dako's rabbit anti human CD3 cross reacts with mouse, or where you looking for a CD3 that only detects mouse? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz at premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: Connolly, Brett M via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 21, 2016 7:59 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CD3 antibody for FFPE mouse tissue Hi all, Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections. I have some old Histonet messages referring to a Neomarkers RB-360 ab which I found through Thermo, but I would like to hear about more recent experiences/recommendations.... Thanks as always, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly at merck.com Tue Jun 21 10:53:16 2016 From: brett_connolly at merck.com (Connolly, Brett M) Date: Tue, 21 Jun 2016 11:53:16 -0400 Subject: [Histonet] CD3 antibody for FFPE mouse tissue In-Reply-To: <14E2C6176416974295479C64A11CB9AE02BEC711583D@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE02BEC711583D@SBS2K8.premierlab.local> Message-ID: Liz, As long as it detects mouse T-cells it's OK. Unfortunately we have a problem getting Dako products through our ordering system. Brett -----Original Message----- From: Elizabeth Chlipala [mailto:liz at premierlab.com] Sent: Tuesday, June 21, 2016 11:20 AM To: Connolly, Brett M; histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] CD3 antibody for FFPE mouse tissue Brett Dako's rabbit anti human CD3 cross reacts with mouse, or where you looking for a CD3 that only detects mouse? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz at premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: Connolly, Brett M via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 21, 2016 7:59 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CD3 antibody for FFPE mouse tissue Hi all, Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections. I have some old Histonet messages referring to a Neomarkers RB-360 ab which I found through Thermo, but I would like to hear about more recent experiences/recommendations.... Thanks as always, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From cforster at umn.edu Tue Jun 21 12:05:10 2016 From: cforster at umn.edu (Colleen Forster) Date: Tue, 21 Jun 2016 12:05:10 -0500 Subject: [Histonet] CD3 antibody for FFPE mouse tissue In-Reply-To: References: <14E2C6176416974295479C64A11CB9AE02BEC711583D@SBS2K8.premierlab.local> Message-ID: Brett, I use the rabbit monoclonal CD3 from Thermo (it was a Lab Vision antibody) with great and consistent results! The hardest part, actually getting the antibody to me....\jeez they have problems with orders sometimes... Here is the information for mine: CD3 (SP7) - cat #RM-9107-S1 I use it at 1:400, citrate retrieval, 1 hour primary incubation. I use the Rabbit on Rodent polymer from Biocare. Respectfully, ' Colleen Forster On Tue, Jun 21, 2016 at 10:53 AM, Connolly, Brett M via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Liz, > As long as it detects mouse T-cells it's OK. Unfortunately we have a > problem getting Dako products through our ordering system. > Brett > > -----Original Message----- > From: Elizabeth Chlipala [mailto:liz at premierlab.com] > Sent: Tuesday, June 21, 2016 11:20 AM > To: Connolly, Brett M; histonet at lists.utsouthwestern.edu > Subject: RE: [Histonet] CD3 antibody for FFPE mouse tissue > > Brett > > Dako's rabbit anti human CD3 cross reacts with mouse, or where you looking > for a CD3 that only detects mouse? > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 881-0763 cell > (303) 682-9060 fax > liz at premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: Connolly, Brett M via Histonet [histonet at lists.utsouthwestern.edu] > Sent: Tuesday, June 21, 2016 7:59 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] CD3 antibody for FFPE mouse tissue > > Hi all, > > Looking for recommendation for an anti-mouse CD3 antibody for FFPE > sections. > > I have some old Histonet messages referring to a Neomarkers RB-360 ab > which I found through Thermo, but I would like to hear about more recent > experiences/recommendations.... > > Thanks as always, > Brett > > Brett M. Connolly, Ph.D. > Prin. Scientist, > Translational Biomarkers - Imaging > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly at merck.com > T- 215-652-2501 > F- 215-993-6803 > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, > New Jersey, USA 07033), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, > New Jersey, USA 07033), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From careerstudio at bellsouth.net Tue Jun 21 12:06:35 2016 From: careerstudio at bellsouth.net (Barbara Siegel) Date: Tue, 21 Jun 2016 13:06:35 -0400 Subject: [Histonet] Histology Laboratory Manager opportunity in Nashville, TN Message-ID: <001b01d1cbdf$453a28c0$cfae7a40$@net> Our client is a fast-paced, large volume clinical laboratory seeking Day-Shift Histology Lab Manager in the Nashville, TN area. This individual will directly oversee 2 supervisors (1 day / 1 night) & will be responsible for all operations / staff of ~22 employees on 3 shifts. Focus will be on day-to-day operations, developing mechanisms to monitor, & improve specimen processing quality & report turnaround times, & maintaining good relationships with customers, physicians, & colleagues. The manager will perform some routine histology tasks of embedding, cutting, IHC's, special stains as needed, depending upon staff & work volume. Accountabilities will include: * Support Client's mission, vision, goals & management decisions. Provide leadership & manage assigned department, solve problems, develop procedures, & conduct / attend meetings. * Continuously monitor & evaluate histology operations & workflow, making changes as needed to assure optimal quality, efficiency, productivity, & turnaround times. Maintain required departmental statistical records & reports. * Communicate with clients, marketing, department supervisors, pathologists, management, & administration regarding laboratory issues. * Manage employee hiring process including developing / updating job descriptions in conjunction with the Human Resources, developing performance expectations, identifying essential functions & knowledge, abilities required for applicable positions. * Manage employee performance by coaching, counseling, motivating, & evaluating employees on a continual basis. Implement disciplinary action as needed & in consultation with Human Resources. Oversee departmental training. * Ensure effective employee relations by sustaining an ethical, non-discriminatory & safe work environment & establishing effective communication lines & methods. Identify & solve employee problems, manage conflict & respond to grievances as needed. * Establish & execute departmental long & short-term goals. Assist in the strategic development of the laboratory services. * Achieve budget profitability objectives through controlling costs and/or increasing market share by effectively utilizing personnel & resources. * Maintain appropriate standards to meet all licensing & regulatory requirements & uphold company policies & integrity. * Perform embedding, microtomy, IHC, & Special stains as needed. * Exercise all laboratory safety precautions & adhere to lab procedures as stated in procedure manuals. * Perform all job responsibilities in alignment with the industry's best security practices & regulatory guidelines to protect the confidentiality, integrity, & availability of protected health information & other sensitive company data. * Stay up-to-date & abide by the Corporate Compliance Program & all corporate policies, including the Privacy & Security policies. Required for this position will be solid supervisory/management expertise in a similar high volume lab with 5+ year prior exp as a histotechnician. Appropriate educational & licensure credentials are needed including ASCP certification, maintenance of continuing education & ability to obtain TN lab license. This highly-visible opportunity offers competitive salary (to be discussed), bonus potential & relocation assistance. Please contact David King at biolabcareers at aol.com for more information. David King Career Studio Biotechnology Div biolabcareers at aol.com 561-738-6363 www.linkedin.com/in/biotechnologyhires/ From kmilne at bccrc.ca Tue Jun 21 12:08:27 2016 From: kmilne at bccrc.ca (Katy Milne) Date: Tue, 21 Jun 2016 17:08:27 +0000 Subject: [Histonet] CD3 antibody for FFPE mouse tissue In-Reply-To: References: Message-ID: <3f859bd982974761a7f7442fcdd29457@CRCMAIL6.BCCRC.CA> Spring Bioscience's SP7 clone works really well for mouse (and human) and is widely available from a number of companies. We use it all the time. Katy Message: 1 Date: Tue, 21 Jun 2016 09:59:58 -0400 From: "Connolly, Brett M" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] CD3 antibody for FFPE mouse tissue Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections. I have some old Histonet messages referring to a Neomarkers RB-360 ab which I found through Thermo, but I would like to hear about more recent experiences/recommendations.... Thanks as always, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 From LRaff at uropartners.com Tue Jun 21 12:25:59 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 21 Jun 2016 17:25:59 +0000 Subject: [Histonet] Post with a Prize (see below or disregard) Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10E2E3A9@COLOEXCH01.uropartners.local> HI All: Although I no longer post blog posts here, this one has a contest with a $25 gift card prize to the winner, so I am making an exception. Good luck! http://www.chicagonow.com/downsize-maybe/2016/06/easy-tuesday-music-trivia-with-a-prize/ Best regards, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From cweston at revealbio.com Tue Jun 21 12:35:04 2016 From: cweston at revealbio.com (Claire Weston) Date: Tue, 21 Jun 2016 17:35:04 +0000 Subject: [Histonet] CD3 antibody for FFPE mouse tissue In-Reply-To: References: Message-ID: <010001557407a05b-db2528cc-78a0-4c53-8107-172ef5298627-000000@email.amazonses.com> Hi Brett, The anti-CD3 antibody from Abcam #ab5690 works really well in FFPE tissue and recognizes mouse. We have a lot of success using that. Hope that helps! Claire Claire Weston, PhD Founder & CEO Reveal Biosciences revealbio.com Tel. 858 274 3663 > > > Hi all, > > Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections. > > I have some old Histonet messages referring to a Neomarkers RB-360 ab which I found through Thermo, but I would like to hear about more recent experiences/recommendations.... > > Thanks as always, > Brett > > Brett M. Connolly, Ph.D. > Prin. Scientist, > Translational Biomarkers - Imaging > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly at merck.com > T- 215-652-2501 > F- 215-993-6803 > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, > New Jersey, USA 07033), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > > > - From jamie.erickson at abbvie.com Tue Jun 21 14:24:16 2016 From: jamie.erickson at abbvie.com (Erickson, Jamie E) Date: Tue, 21 Jun 2016 19:24:16 +0000 Subject: [Histonet] CD3 antibody for mouse FFPE Message-ID: <37ed17655eaf4352884cbb4cf6950a62@USAASECSM025.R0018.COLLABORATION.ECS.HP.COM> Hi Brett, We run the Leica BOND RX here and we love the CD3 from Thermo CD3 (Clone SP7) Rabbit Monoclonal Antibody Cat. #RM-9107-S0, -S1, or -S (0.1ml, 0.5ml, or 1.0ml Supernatant). We use the H2-20 retrieval which is HIER (EDTA) and it works on human , mouse and Rat. It is a Supernatant so you'll have to call for the ~ concentration it is ~160ug/ml and works at 0.8ug/ml. With the anti-rabbit polymer detection it looks great and the incubated time we use is only 15min. Another good thing is we do not need to use a protein block which saves time. It looks great as a double with IBA-1 Alk phos and CD3 black (biocare Deep space black) and a light methyl green counter stain. Hope that helps, Jamie Erickson, MS Scientist AbbVie Bioresearch Center, Inc. 100 Research Drive Worcester, MA 01605 Ph: 508-688-3134 From jvickroy at SpringfieldClinic.com Tue Jun 21 14:27:32 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Tue, 21 Jun 2016 19:27:32 +0000 Subject: [Histonet] HT Certification Message-ID: <9B1A1501A800064397369BD8072E6BCA06574A67@E2K10DB.springfieldclinic.com> I just got through meeting with HR regarding a salary incentive for employees that successfully pass their HT certification. All of us are aware that many histology labs have employees that are not certified. We are a small clinic lab that was set up about a year and a half ago. When we first set up the lab we were able to bring three qualified HT's from another lab locally. The lab has continued to grow since then and now we have 4 other staff members that are performing HT tasks including microtomy, H&E staining, and grossing (they all have BS degrees). They were hired believing that once they were eligible to take the HT certification that they would take the test and if they passed they would get a 5% salary adjustment. However the incentive or 5% increase was not written into the job description. Currently the clinic purchases the study materials (self-instruction program) for the staff but we use very little work time for instructional purposes. The staff are expected to study on their own, pay for the exam, and take the test. If they do not pass the test in the period of one year after completing the on-the-job training then technically we could tell them they are no longer employed. However where would that leave us given we couldn't find any additional certified HT's to hire in the first place. We would have to start over again with another untrained BS graduate. One of the question I was asked in the HR meeting was, "What duties can a certified HT do that a non-certified technician cannot?". Since all of our staff have BS degrees in biology and have all received gross training (90 days) I wasn't sure there was anything else that they couldn't do in the lab that only a certified HT could do. I wish there were many duties. I am afraid that if we don't have some certification requirements then in a few years we will have very few HT's except those wanting to be supervisory. We are CAP certified. Is anybody aware of certain HT duties that can and should only be done by a certified HT or HTL? I know the high complexity requirement in grossing but this is based upon 90 days of training and a set number of science courses (biology and chemistry), and not certification. I know that some institutions have handled the incentive to take the HT certification by hiring new staff as HT trainees and then if they passed the HT certification they move into another job class which has a higher salary range. This is an option that may be done in the future here but unfortunately that was not set up initially here at the clinic. I also know of institutions that have discouraged BS degree staff from taking the HT certification exam thinking that if they become certified they will find a job elsewhere. So I am trying to list the advantages of staff becoming certified. The clinic in particular wants to know what they get for the 5% increase if someone passes the certification. Any ideas how to respond? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From brett_connolly at merck.com Tue Jun 21 14:44:46 2016 From: brett_connolly at merck.com (Connolly, Brett M) Date: Tue, 21 Jun 2016 15:44:46 -0400 Subject: [Histonet] Thanks! CD3 antibody for mouse IHC Message-ID: Thanks to all who replied to my CD3 query! The responses were just what I was hoping for. Best regards, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Timothy.Morken at ucsf.edu Tue Jun 21 14:57:32 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 21 Jun 2016 19:57:32 +0000 Subject: [Histonet] HT Certification In-Reply-To: <9B1A1501A800064397369BD8072E6BCA06574A67@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA06574A67@E2K10DB.springfieldclinic.com> Message-ID: <761E2B5697F795489C8710BCC72141FF6FD6155C@ex07.net.ucsf.edu> I guess one question is, how did they get the impression they would get the 5%? That is not something that is normally written into the job description, but should be documented in a job offer, or it is made clear in some policy that as they gain competency, and the lab organization accommodates it, they will move up the ladder. . Was that written in or verbally given? The key is something you already mentioned: job classification. To move to a higher classification they need to learn the tasks for that classification. They need to do the tasks of that job while training so are technically doing the work. However, the difference is that while training they are under close supervision. Once they pass competency to do the work they can work with general supervision. And they get the raise. So if they start at entry level that does not require HT, the next level up does require it and pays more. That gives incentive to get the certification. This pertains to any position. Only those at a certain classification are allowed to do the work of that classification under general supervision. So, once they pass that competency you reclassify them to a higher category. We have histotech 1, 2, 3, 4. 1 and 2 are bench techs doing routine work. 3 is a senior tech who can do test development and validation and write and edit SOP's. 4 is a Lead tech and expected to supervise a few people and organize daily work. Supervisor is above that. Once I am sure a person can do 3 work, and I need a 3-level tech, I will reclassify them. They get more responsibility and more pay. That is just the fair way to do it. You decide what the jobs tasks are in those classifications. So you can tell HR that level 1 an 2 do XXX and level 3 does XXX plus YYY. We don't make everyone a 3 just because they have been here a long time or can do certain things. We limit the number we have (you need a certain number of people who do the basic work!). If I have someone who is capable of being a 3, but cannot accommodate them in the organization, I will give them special short term projects, or find some way to let them do a bit more. However some may leave if they can find another job that will give them what they want. That is just the way it is sometimes. Tim -----Original Message----- From: Vickroy, James via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 21, 2016 12:28 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] HT Certification I just got through meeting with HR regarding a salary incentive for employees that successfully pass their HT certification. All of us are aware that many histology labs have employees that are not certified. We are a small clinic lab that was set up about a year and a half ago. When we first set up the lab we were able to bring three qualified HT's from another lab locally. The lab has continued to grow since then and now we have 4 other staff members that are performing HT tasks including microtomy, H&E staining, and grossing (they all have BS degrees). They were hired believing that once they were eligible to take the HT certification that they would take the test and if they passed they would get a 5% salary adjustment. However the incentive or 5% increase was not written into the job description. Currently the clinic purchases the study materials (self-instruction program) for the staff but we use very little work time for instructional purposes. The staff are expected to study on their own, pay for the exam, and take the test. If they do not pass the test in the period of one year after completing the on-the-job training then technically we could tell them they are no longer employed. However where would that leave us given we couldn't find any additional certified HT's to hire in the first place. We would have to start over again with another untrained BS graduate. One of the question I was asked in the HR meeting was, "What duties can a certified HT do that a non-certified technician cannot?". Since all of our staff have BS degrees in biology and have all received gross training (90 days) I wasn't sure there was anything else that they couldn't do in the lab that only a certified HT could do. I wish there were many duties. I am afraid that if we don't have some certification requirements then in a few years we will have very few HT's except those wanting to be supervisory. We are CAP certified. Is anybody aware of certain HT duties that can and should only be done by a certified HT or HTL? I know the high complexity requirement in grossing but this is based upon 90 days of training and a set number of science courses (biology and chemistry), and not certification. I know that some institutions have handled the incentive to take the HT certification by hiring new staff as HT trainees and then if they passed the HT certification they move into another job class which has a higher salary range. This is an option that may be done in the future here but unfortunately that was not set up initially here at the clinic. I also know of institutions that have discouraged BS degree staff from taking the HT certification exam thinking that if they become certified they will find a job elsewhere. So I am trying to list the advantages of staff becoming certified. The clinic in particular wants to know what they get for the 5% increase if someone passes the certification. Any ideas how to respond? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jo-ann.bader at mcgill.ca Tue Jun 21 15:30:54 2016 From: jo-ann.bader at mcgill.ca (Jo-Ann Bader, Ms.) Date: Tue, 21 Jun 2016 20:30:54 +0000 Subject: [Histonet] Histonet Digest, Vol 151, Issue 18 In-Reply-To: References: Message-ID: We are happy the ABCAM CD3 Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: June 21, 2016 12:00:02 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 151, Issue 18 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. CD3 antibody for FFPE mouse tissue (Connolly, Brett M) 2. Re: CD3 antibody for FFPE mouse tissue (Elizabeth Chlipala) 3. Re: CD3 antibody for FFPE mouse tissue (Connolly, Brett M) ---------------------------------------------------------------------- Message: 1 Date: Tue, 21 Jun 2016 09:59:58 -0400 From: "Connolly, Brett M" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] CD3 antibody for FFPE mouse tissue Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections. I have some old Histonet messages referring to a Neomarkers RB-360 ab which I found through Thermo, but I would like to hear about more recent experiences/recommendations.... Thanks as always, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 2 Date: Tue, 21 Jun 2016 09:19:49 -0600 From: Elizabeth Chlipala To: "Connolly, Brett M" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] CD3 antibody for FFPE mouse tissue Message-ID: <14E2C6176416974295479C64A11CB9AE02BEC711583D at SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Brett Dako's rabbit anti human CD3 cross reacts with mouse, or where you looking for a CD3 that only detects mouse? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz at premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: Connolly, Brett M via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 21, 2016 7:59 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CD3 antibody for FFPE mouse tissue Hi all, Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections. I have some old Histonet messages referring to a Neomarkers RB-360 ab which I found through Thermo, but I would like to hear about more recent experiences/recommendations.... Thanks as always, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 21 Jun 2016 11:53:16 -0400 From: "Connolly, Brett M" To: Elizabeth Chlipala , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] CD3 antibody for FFPE mouse tissue Message-ID: Content-Type: text/plain; charset="us-ascii" Liz, As long as it detects mouse T-cells it's OK. Unfortunately we have a problem getting Dako products through our ordering system. Brett -----Original Message----- From: Elizabeth Chlipala [mailto:liz at premierlab.com] Sent: Tuesday, June 21, 2016 11:20 AM To: Connolly, Brett M; histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] CD3 antibody for FFPE mouse tissue Brett Dako's rabbit anti human CD3 cross reacts with mouse, or where you looking for a CD3 that only detects mouse? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz at premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: Connolly, Brett M via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 21, 2016 7:59 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CD3 antibody for FFPE mouse tissue Hi all, Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections. I have some old Histonet messages referring to a Neomarkers RB-360 ab which I found through Thermo, but I would like to hear about more recent experiences/recommendations.... Thanks as always, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 151, Issue 18 ***************************************** From jaylundgren at gmail.com Tue Jun 21 16:29:06 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Tue, 21 Jun 2016 14:29:06 -0700 Subject: [Histonet] HT Certification In-Reply-To: <761E2B5697F795489C8710BCC72141FF6FD6155C@ex07.net.ucsf.edu> References: <9B1A1501A800064397369BD8072E6BCA06574A67@E2K10DB.springfieldclinic.com> <761E2B5697F795489C8710BCC72141FF6FD6155C@ex07.net.ucsf.edu> Message-ID: Maybe someone can quote CLIA chapter and verse, but, in my understanding, only registered HTs or HTLs are supposed to be doing embedding, cutting, special stains and IHC unsupervised. I think the regulation says non-registered personnel (trainees) can perform these duties only under direct supervision. It all depends on how you define direct supervision. To me, direct supervision means someone standing over your shoulder. However, it has been explained to me that as long as the people working are being trained toward the exam, it is the responsibility of the Medical Director of the lab to make sure they are doing the work adequately. So most places interpret this as, "Come get me if you have any questions." I run into unregistered, OJT techs all the time. And some of them are good techs. And some people get upset when this subject comes up on Histonet, because they feel like they do the job just as well as someone who graduated from an NAACLS training program and has passed the test. As you said, this is becoming more and more common, and in my opinion, is the main reason wages are still low, which in turn is the reason why there is a chronic shortage of histotechs. I think you should pay the unregistered trainees minimum wage, sign a contract stating they must pass the test within 18 months to continue working, then raise their pay to $30./hr when they do (at least, I don't know the cost of living in Springfield). Carrot AND stick. 5% is peanuts, it won't motivate anyone. But that's just my opinion. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Tue, Jun 21, 2016 at 12:57 PM, Morken, Timothy via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I guess one question is, how did they get the impression they would get > the 5%? That is not something that is normally written into the job > description, but should be documented in a job offer, or it is made clear > in some policy that as they gain competency, and the lab organization > accommodates it, they will move up the ladder. . Was that written in or > verbally given? > > The key is something you already mentioned: job classification. To move to > a higher classification they need to learn the tasks for that > classification. They need to do the tasks of that job while training so are > technically doing the work. However, the difference is that while training > they are under close supervision. Once they pass competency to do the work > they can work with general supervision. And they get the raise. So if they > start at entry level that does not require HT, the next level up does > require it and pays more. That gives incentive to get the certification. > > This pertains to any position. Only those at a certain classification are > allowed to do the work of that classification under general supervision. > So, once they pass that competency you reclassify them to a higher > category. We have histotech 1, 2, 3, 4. 1 and 2 are bench techs doing > routine work. 3 is a senior tech who can do test development and validation > and write and edit SOP's. 4 is a Lead tech and expected to supervise a few > people and organize daily work. Supervisor is above that. Once I am sure a > person can do 3 work, and I need a 3-level tech, I will reclassify them. > They get more responsibility and more pay. That is just the fair way to do > it. > > You decide what the jobs tasks are in those classifications. So you can > tell HR that level 1 an 2 do XXX and level 3 does XXX plus YYY. > > We don't make everyone a 3 just because they have been here a long time or > can do certain things. We limit the number we have (you need a certain > number of people who do the basic work!). If I have someone who is capable > of being a 3, but cannot accommodate them in the organization, I will give > them special short term projects, or find some way to let them do a bit > more. However some may leave if they can find another job that will give > them what they want. That is just the way it is sometimes. > > > Tim > > > -----Original Message----- > From: Vickroy, James via Histonet [mailto: > histonet at lists.utsouthwestern.edu] > Sent: Tuesday, June 21, 2016 12:28 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] HT Certification > > I just got through meeting with HR regarding a salary incentive for > employees that successfully pass their HT certification. All of us are > aware that many histology labs have employees that are not certified. We > are a small clinic lab that was set up about a year and a half ago. When > we first set up the lab we were able to bring three qualified HT's from > another lab locally. The lab has continued to grow since then and now we > have 4 other staff members that are performing HT tasks including > microtomy, H&E staining, and grossing (they all have BS degrees). They > were hired believing that once they were eligible to take the HT > certification that they would take the test and if they passed they would > get a 5% salary adjustment. However the incentive or 5% increase was not > written into the job description. > > Currently the clinic purchases the study materials (self-instruction > program) for the staff but we use very little work time for instructional > purposes. The staff are expected to study on their own, pay for the exam, > and take the test. > If they do not pass the test in the period of one year after completing > the on-the-job training then technically we could tell them they are no > longer employed. However where would that leave us given we couldn't find > any additional certified HT's to hire in the first place. We would have to > start over again with another untrained BS graduate. > > One of the question I was asked in the HR meeting was, "What duties can a > certified HT do that a non-certified technician cannot?". Since all of > our staff have BS degrees in biology and have all received gross training > (90 days) I wasn't sure there was anything else that they couldn't do in > the lab that only a certified HT could do. I wish there were many > duties. I am afraid that if we don't have some certification requirements > then in a few years we will have very few HT's except those wanting to be > supervisory. We are CAP certified. Is anybody aware of certain HT > duties that can and should only be done by a certified HT or HTL? I know > the high complexity requirement in grossing but this is based upon 90 days > of training and a set number of science courses (biology and chemistry), > and not certification. > > I know that some institutions have handled the incentive to take the HT > certification by hiring new staff as HT trainees and then if they passed > the HT certification they move into another job class which has a higher > salary range. This is an option that may be done in the future here but > unfortunately that was not set up initially here at the clinic. I also > know of institutions that have discouraged BS degree staff from taking the > HT certification exam thinking that if they become certified they will > find a job elsewhere. > > So I am trying to list the advantages of staff becoming certified. The > clinic in particular wants to know what they get for the 5% increase if > someone passes the certification. Any ideas how to respond? > > Jim > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvickroy at SpringfieldClinic.com jvickroy at SpringfieldClinic.com> > > > > This electronic message contains information from Springfield Clinic, LLP > that may be confidential, privileged, and/or sensitive. This information is > intended for the use of the individual(s) or entity(ies) named above. If > you are not the intended recipient, be aware that disclosure, copying, > distribution, or action taken on the contents of this information is > strictly prohibited. If you have received this electronic message in error, > please notify the sender immediately, by electronic mail, so that > arrangements may be made for the retrieval of this electronic message. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mills at 3scan.com Tue Jun 21 16:39:19 2016 From: mills at 3scan.com (Caroline Miller) Date: Tue, 21 Jun 2016 14:39:19 -0700 Subject: [Histonet] CD3 antibody for FFPE mouse tissue In-Reply-To: <14E2C6176416974295479C64A11CB9AE02BEC711583D@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE02BEC711583D@SBS2K8.premierlab.local> Message-ID: +1 to the Dako antibody, it is the only one I have ever found to work (but I have never used them on the automated staining systems, only old-skool by hand or sequenza) mills On Tue, Jun 21, 2016 at 8:19 AM, Elizabeth Chlipala via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Brett > > Dako's rabbit anti human CD3 cross reacts with mouse, or where you looking > for a CD3 that only detects mouse? > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 881-0763 cell > (303) 682-9060 fax > liz at premierlab.com > > Ship to address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > ________________________________________ > From: Connolly, Brett M via Histonet [histonet at lists.utsouthwestern.edu] > Sent: Tuesday, June 21, 2016 7:59 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] CD3 antibody for FFPE mouse tissue > > Hi all, > > Looking for recommendation for an anti-mouse CD3 antibody for FFPE > sections. > > I have some old Histonet messages referring to a Neomarkers RB-360 ab > which I found through Thermo, but I would like to hear about more recent > experiences/recommendations.... > > Thanks as always, > Brett > > Brett M. Connolly, Ph.D. > Prin. Scientist, > Translational Biomarkers - Imaging > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly at merck.com > T- 215-652-2501 > F- 215-993-6803 > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, > New Jersey, USA 07033), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From supervisor at galahistolab.com Tue Jun 21 16:55:06 2016 From: supervisor at galahistolab.com (Rachel Pinch) Date: Tue, 21 Jun 2016 16:55:06 -0500 Subject: [Histonet] Automatic ordering Message-ID: <01bc01d1cc07$92915350$b7b3f9f0$@galahistolab.com> Does anyone have a pathologist that requires a special stain or HP IHC's ran on ALL upper GI cases? Is it overutilization or do you feel it is for verification purposes? Rachel Pinch, HT(ASCP) Laboratory Operations Manager GALA Histology Lab 3030 S. Gessner Suite 290 Houston, Texas 77063 From Forest.Blankenship at dchstx.org Wed Jun 22 10:19:40 2016 From: Forest.Blankenship at dchstx.org (Forest Blankenship) Date: Wed, 22 Jun 2016 15:19:40 +0000 Subject: [Histonet] Rachel Pinch, HT(ASCP) Message-ID: <8D5A5A0BF8A80F43A6E52872E56F523C0138EC9B@dchmxdb02.driscoll.dch> Rachel; if you are talking about testing for H. Pylori on gastric samples it can be justified as a best practice. I was surprised by how many kids have that bacterium when I moved down to Corpus to work in a pediatric hospital. I do miss H-town and it's theater scene. Best wishes, F. Kevin Blankenship, HTL Disclaimer: This email and its content are confidential and intended solely for the use of the addressee. Please notify the sender if you have received this email in error or simply delete it. From Ronald.Houston at nationwidechildrens.org Wed Jun 22 10:27:35 2016 From: Ronald.Houston at nationwidechildrens.org (Houston, Ronald) Date: Wed, 22 Jun 2016 15:27:35 +0000 Subject: [Histonet] Rachel Pinch, HT(ASCP) In-Reply-To: <8D5A5A0BF8A80F43A6E52872E56F523C0138EC9B@dchmxdb02.driscoll.dch> References: <8D5A5A0BF8A80F43A6E52872E56F523C0138EC9B@dchmxdb02.driscoll.dch> Message-ID: Have to disagree with performing H.pylori testing on all gastric biopsies. It cannot be justified as best practice if there is no clinical or pathological indication. Ronnie Houston, MS HT(ASCP)QIHC FIBMS Anatomic Pathology Manager Laboratory Services Nationwide Chidlren's Hospital 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston at nationwidechildrens.org www.NationwideChildrens.org "Without continual growth and progress, such words as improvement, achievement, and success have no meaning." ~ Ben Franklin -----Original Message----- From: Forest Blankenship via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, June 22, 2016 11:20 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Rachel Pinch, HT(ASCP) Rachel; if you are talking about testing for H. Pylori on gastric samples it can be justified as a best practice. I was surprised by how many kids have that bacterium when I moved down to Corpus to work in a pediatric hospital. I do miss H-town and it's theater scene. Best wishes, F. Kevin Blankenship, HTL Disclaimer: This email and its content are confidential and intended solely for the use of the addressee. Please notify the sender if you have received this email in error or simply delete it. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=CwICAg&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=zB8FaApei1RwQ8ORwLdjajyCOmTOi0UNE4lXgqqJEz4&s=G7To9YB1qndWj6KZZjv4bfNQL74VbIfiXl9d3z8DlEw&e= From tbraud at holyredeemer.com Wed Jun 22 11:51:51 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 22 Jun 2016 16:51:51 +0000 Subject: [Histonet] HT Certification Message-ID: <48E053DDF6CE074DB6A7414BA05403F807BDBB@HRHEX02-HOS.holyredeemer.local> Wow, you sure picked a doozy of a subject. Unless you work in a state that has specific licensure requirements, there really is nothing to prevent a non-certified Histology tech from doing the same duties as a certified tech In my opinion, it's more a matter of skill sets, and as we all know, there are certified techs that couldn't cut their way out of a paper bag, just as there are non-certified techs that are Histology wizards. The question is one of training and commitment. I do think that there are certain duties that could be set aside for certified techs, such as validation of controls and antibodies, Quality Assurance activity monitoring. The difference will always be those who understand the theory behind the work. At least with certification, there is a minimal standard for training and commitment. A good indicator of your institution's commitment to certification is their hiring practices for phlebotomy or Nurses aids - Certified or not? If you have them and they are certified, then ask why. Histotechs have always been the evil step children of the laboratory system. Your institution should take pride in having a certified staff, but if they don't care, it will be an uphill battle to instill that commitment. Best of luck, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Today's Topics: 7. HT Certification (Vickroy, James) From ewj at pigsqq.org Wed Jun 22 21:48:39 2016 From: ewj at pigsqq.org (=?utf-8?Q?ewj?=) Date: Thu, 23 Jun 2016 10:48:39 +0800 Subject: [Histonet] =?utf-8?q?Lith_vs_Sod?= Message-ID: <20160623024839.15327.qmail@station195.com> Some procedures call for Lithium carbonate. Others seem to say that Na2CO3 could be used. What is the advantage of using Lithium carbonate vs Sodium Carbonate? From Thomas.Huynh at harrishealth.org Thu Jun 23 10:48:43 2016 From: Thomas.Huynh at harrishealth.org (Huynh, Thomas) Date: Thu, 23 Jun 2016 15:48:43 +0000 Subject: [Histonet] Shandon Varistain Gemini Slide Stainer Message-ID: <5E029F2DE803CF48B297533BEC62764A018600C9DD@LBMSG02.hchd.local> Hi All, Does anyone know the life expectancy of the Gemini H&E stainer? My boss has asked me this question because we are in the process of requesting for a new one. Thomas Thomas Huynh BS, HT (ASCP) Histology Lab Supervisor |Department of Pathology HARRISHEALTH SYSTEM 5656 Kelly Street Houston, Tx 77026 Lyndon B. Johnson General Hospital (LBJGH) O: 713.566.5282 | F: 713.566.5285 | P: 713.297.1606 | Thomas.Huynh at harrishealth.org CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From Pez at uwyo.edu Thu Jun 23 12:04:19 2016 From: Pez at uwyo.edu (Rebecca E. Ashley) Date: Thu, 23 Jun 2016 17:04:19 +0000 Subject: [Histonet] Marking Tissues with Eosin Message-ID: I had a biopsy today that was nearly impossible to see on the sponges during embedding or in the block. I've heard mention of marking these with eosin to make them easier to see. Has anyone done this? Or do you use some other type of marking dye for this purpose? Thanks for your input! Rebecca Rebecca Ashley Histotechnologist Wyoming State Vet Lab 1174 Snowy Range Rd. Laramie, WY 82070 Phone: 307-766-9946 Fax: 307-721-2051 From mwerdler at gmail.com Thu Jun 23 12:49:37 2016 From: mwerdler at gmail.com (Mca Werdler) Date: Thu, 23 Jun 2016 12:49:37 -0500 Subject: [Histonet] Marking Tissues with Eosin In-Reply-To: References: Message-ID: Dear Rebecca, Yes this is possible. just don't use a too strong concentration. The eosin should give a slight pink color on the tissue after processing and after embedding. Good luck, Maarten 2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet < histonet at lists.utsouthwestern.edu>: > I had a biopsy today that was nearly impossible to see on the sponges > during embedding or in the block. I've heard mention of marking these with > eosin to make them easier to see. Has anyone done this? Or do you use > some other type of marking dye for this purpose? > Thanks for your input! > Rebecca > > Rebecca Ashley > Histotechnologist > Wyoming State Vet Lab > 1174 Snowy Range Rd. > Laramie, WY 82070 > Phone: 307-766-9946 > Fax: 307-721-2051 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tejohnson at genoptix.com Thu Jun 23 14:04:44 2016 From: tejohnson at genoptix.com (Teri Johnson) Date: Thu, 23 Jun 2016 19:04:44 +0000 Subject: [Histonet] Shandon Varistain Gemini Slide Stainer Message-ID: Hi Thomas, Are you looking for true life expectancy or what is reported for depreciation? In my experience, most tissue processors never die, but only need to be retired due to lack of available support/parts or because a lab requires newer technology. Also in my experience, a "better" model comes out soon after I have purchased one. So the life span is probably 20 years after you wished you had a different one. :-) Best wishes, Teri Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com ------------------------------ Hi All, Does anyone know the life expectancy of the Gemini H&E stainer? My boss has asked me this question because we are in the process of requesting for a new one. Thomas Thomas Huynh BS, HT (ASCP) Histology Lab Supervisor |Department of Pathology HARRISHEALTH SYSTEM 5656 Kelly Street Houston, Tx 77026 Lyndon B. Johnson General Hospital (LBJGH) O: 713.566.5282 | F: 713.566.5285 | P: 713.297.1606 | Thomas.Huynh at harrishealth.org CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From j.rowaihi at alborglaboratories.com Thu Jun 23 14:56:34 2016 From: j.rowaihi at alborglaboratories.com (Jamal Rowaihi) Date: Thu, 23 Jun 2016 22:56:34 +0300 Subject: [Histonet] Marking Tissues with Eosin Message-ID: Hi?If you are using buffered Formaline so the Eosin color will not resist until the end of tissue processing.I recommend to add small amount of stock Eosin to the last alcohol in theTissue processor.? Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories?Sent from my cell phone -------- Original message --------From: Mca Werdler via Histonet Date: 6/23/16 8:49 PM (GMT+03:00) To: "Rebecca E. Ashley" Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Marking Tissues with Eosin Dear Rebecca, Yes this is possible. just don't use a too strong concentration. The eosin should give a slight pink color on the tissue after processing and after embedding. Good luck, Maarten 2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet < histonet at lists.utsouthwestern.edu>: > I had a biopsy today that was nearly impossible to see on the sponges > during embedding or in the block.? I've heard mention of marking these with > eosin to make them easier to see.? Has anyone done this?? Or do you use > some other type of marking dye for this purpose? > Thanks for your input! > Rebecca > > Rebecca Ashley > Histotechnologist > Wyoming State Vet Lab > 1174 Snowy Range Rd. > Laramie, WY 82070 > Phone: 307-766-9946 > Fax: 307-721-2051 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paula at excaliburpathology.com Thu Jun 23 16:02:49 2016 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Thu, 23 Jun 2016 21:02:49 +0000 (UTC) Subject: [Histonet] Shandon Varistain Gemini Slide Stainer In-Reply-To: References: Message-ID: <994846485.545115.1466715770021.JavaMail.yahoo@mail.yahoo.com> This is so true! I had a Fisher Histomatic automated slide stainer that was manufactured in 1985 that I used until the building took a direct lightning strike JUST LAST YEAR! My old VIP is still going! ?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Teri Johnson via Histonet To: "histonet at lists.utsouthwestern.edu" Sent: Thursday, June 23, 2016 2:04 PM Subject: Re: [Histonet] Shandon Varistain Gemini Slide Stainer Hi Thomas, Are you looking for true life expectancy or what is reported for depreciation? In my experience, most tissue processors never die, but only need to be retired due to lack of available support/parts or because a lab requires newer technology. Also in my experience, a "better" model comes out soon after I have purchased one. So the life span is probably 20 years after you wished you had a different one. :-) Best wishes, Teri Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA? 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com ------------------------------ Hi All, Does anyone know the life expectancy of the Gemini H&E stainer? My boss has asked me this question because we are in the process of requesting for a new one. Thomas Thomas Huynh? BS, HT (ASCP) Histology Lab Supervisor |Department of Pathology HARRISHEALTH SYSTEM 5656 Kelly Street Houston, Tx 77026 Lyndon B. Johnson General Hospital (LBJGH) O: 713.566.5282 | F: 713.566.5285 | P: 713.297.1606 | Thomas.Huynh at harrishealth.org CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CBird at amli-denton.com Thu Jun 23 16:20:45 2016 From: CBird at amli-denton.com (Cindy Bird) Date: Thu, 23 Jun 2016 16:20:45 -0500 Subject: [Histonet] Marking Tissues with Eosin In-Reply-To: References: Message-ID: We place a small drop of concentrate straight on tissue. Sent from my iPhone > On Jun 23, 2016, at 12:56 PM, Mca Werdler via Histonet wrote: > > Dear Rebecca, > > Yes this is possible. just don't use a too strong concentration. The eosin > should give a slight pink color on the tissue after processing and after > embedding. > > Good luck, > > Maarten > > 2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet < > histonet at lists.utsouthwestern.edu>: > >> I had a biopsy today that was nearly impossible to see on the sponges >> during embedding or in the block. I've heard mention of marking these with >> eosin to make them easier to see. Has anyone done this? Or do you use >> some other type of marking dye for this purpose? >> Thanks for your input! >> Rebecca >> >> Rebecca Ashley >> Histotechnologist >> Wyoming State Vet Lab >> 1174 Snowy Range Rd. >> Laramie, WY 82070 >> Phone: 307-766-9946 >> Fax: 307-721-2051 >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robinsoc at mercyhealth.com Fri Jun 24 08:19:43 2016 From: robinsoc at mercyhealth.com (Cynthia Robinson) Date: Fri, 24 Jun 2016 13:19:43 +0000 Subject: [Histonet] Marking Tissues with Eosin In-Reply-To: References: , Message-ID: <4EE642D353925D4D96CB95E12427DBAE56DD4CF9@NODCMSTMBX06.no.trinity-health.org> We use safranin at the grossing station and it is a dark pink at embedding. Works really well in our hands. Added plus is no fluorescent issues that you can have with eosin. Cindi ________________________________________ From: Cindy Bird via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, June 23, 2016 4:20 PM To: Mca Werdler Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Marking Tissues with Eosin We place a small drop of concentrate straight on tissue. Sent from my iPhone > On Jun 23, 2016, at 12:56 PM, Mca Werdler via Histonet wrote: > > Dear Rebecca, > > Yes this is possible. just don't use a too strong concentration. The eosin > should give a slight pink color on the tissue after processing and after > embedding. > > Good luck, > > Maarten > > 2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet < > histonet at lists.utsouthwestern.edu>: > >> I had a biopsy today that was nearly impossible to see on the sponges >> during embedding or in the block. I've heard mention of marking these with >> eosin to make them easier to see. Has anyone done this? Or do you use >> some other type of marking dye for this purpose? >> Thanks for your input! >> Rebecca >> >> Rebecca Ashley >> Histotechnologist >> Wyoming State Vet Lab >> 1174 Snowy Range Rd. >> Laramie, WY 82070 >> Phone: 307-766-9946 >> Fax: 307-721-2051 >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From rsrichmond at gmail.com Fri Jun 24 12:31:31 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 24 Jun 2016 13:31:31 -0400 Subject: [Histonet] Marking Tissues with Eosin Message-ID: Rebecca Ashley at the Wyoming State Vet Lab in Laramie asks >> I had a biopsy today that was nearly impossible to see on the sponges during embedding or in the block. I've heard mention of marking these with eosin to make them easier to see. Has anyone done this? Or do you use some other type of marking dye for this purpose?<< You must not use eosin (or other fluorescent dyes such as Mercurochrome) to mark tissue during grossing - the persistent dye interferes with FISH - you don't want eosin in your tissue processing system at all. As Cindi (where?) notes >>We use safranin at the grossing station and it is a dark pink at embedding. Works really well in our hands. Added plus is no fluorescent issues that you can have with eosin.<< I've found safranin O (not saffron) to be highly satisfactory, and it is not fluorescent. A solution of it is used by microbiology labs as the counterstain for the Gram stain, and this preparation is highly satisfactory and you don't have to buy anything if the lab across the hall already has it. I've heard of using hematoxylin for this purpose, but have never seen it done. Bob Richmond Samurai Pathologist Maryville TN From CDavis at che-east.org Fri Jun 24 13:19:29 2016 From: CDavis at che-east.org (Cassie P. Davis) Date: Fri, 24 Jun 2016 18:19:29 +0000 Subject: [Histonet] Eosin to help see tissue better Message-ID: <5C815EADE724D14AA0CC8F037C4185F079B32628@SB01MSTMBX13.sb.trinity-health.org> Hello Rebecca, Reguarding the following message: "I had a biopsy today that was nearly impossible to see on the sponges during embedding or in the block. I've heard mention of marking these with eosin to make them easier to see. Has anyone done this? Or do you use some other type of marking dye for this purpose? Thanks for your input! Rebecca" I have worked at a few places that have put 4-10 cc in the last alcohol of the processor and it is a great help with those tiny, white biopsies and the very thin prostate biopsy cores. It is put in the last alcohol because if you put it any of the solutions before the last alcohol it will be washed out. It does not overstain your tissue because it is removed during the depariffination and dehydration steps before staining. A word of caution, we now have a Peloris processor and were told if we put Eosin on the processor it will invalidate our warranty so I would check before doing it. We greatly miss that pink tint in the tissue, it made it much easier to embed and face as well as section. Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From taynay143 at hotmail.com Fri Jun 24 13:20:07 2016 From: taynay143 at hotmail.com (Tyra Connor) Date: Fri, 24 Jun 2016 18:20:07 +0000 Subject: [Histonet] Crystal Violet Message-ID: We do the simple, quick crystal violet for amyloid and one of the Drs. would like for it to be lighter. We make stock soln from powder, and then fresh working soln each time. Using a procedure from the 1990 Carson (pg 134-135). Basically the slides get dipped and then rinsed. There's no differentiation step, and we've tried leaving it on for less time, but the tissue just grabs the crystal violet so readily. Any suggestions would be much appreciated. Thanks and enjoy the weekend, t. From haemeotoxylin at hotmail.com Fri Jun 24 14:43:45 2016 From: haemeotoxylin at hotmail.com (lisa ryan) Date: Fri, 24 Jun 2016 19:43:45 +0000 Subject: [Histonet] Marking tissue with eosin In-Reply-To: References: Message-ID: We add 50mls of 1% eosin to our first alcohol after the formalin. 1% EOSIN FOR PROCESSING BIOPSIES 100mL ACETIC ACID 2g EOSIN 100mL DISTILLED WATER Stir well! Lisa Ryan Histology St James hospital Dublin Ireland ________________________________________ From: histonet-request at lists.utsouthwestern.edu [histonet-request at lists.utsouthwestern.edu] Sent: 24 June 2016 17:00 To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 151, Issue 22 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Marking Tissues with Eosin (Rebecca E. Ashley) 2. Re: Marking Tissues with Eosin (Mca Werdler) 3. Re: Shandon Varistain Gemini Slide Stainer (Teri Johnson) 4. Re: Marking Tissues with Eosin (Jamal Rowaihi) 5. Re: Shandon Varistain Gemini Slide Stainer (Paula Keene Pierce) 6. Re: Marking Tissues with Eosin (Cindy Bird) 7. Re: Marking Tissues with Eosin (Cynthia Robinson) ---------------------------------------------------------------------- Message: 1 Date: Thu, 23 Jun 2016 17:04:19 +0000 From: "Rebecca E. Ashley" To: "Histonet at lists.utsouthwestern.edu" Subject: [Histonet] Marking Tissues with Eosin Message-ID: Content-Type: text/plain; charset="us-ascii" I had a biopsy today that was nearly impossible to see on the sponges during embedding or in the block. I've heard mention of marking these with eosin to make them easier to see. Has anyone done this? Or do you use some other type of marking dye for this purpose? Thanks for your input! Rebecca Rebecca Ashley Histotechnologist Wyoming State Vet Lab 1174 Snowy Range Rd. Laramie, WY 82070 Phone: 307-766-9946 Fax: 307-721-2051 ------------------------------ Message: 2 Date: Thu, 23 Jun 2016 12:49:37 -0500 From: Mca Werdler To: "Rebecca E. Ashley" Cc: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Marking Tissues with Eosin Message-ID: Content-Type: text/plain; charset=UTF-8 Dear Rebecca, Yes this is possible. just don't use a too strong concentration. The eosin should give a slight pink color on the tissue after processing and after embedding. Good luck, Maarten 2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet < histonet at lists.utsouthwestern.edu>: > I had a biopsy today that was nearly impossible to see on the sponges > during embedding or in the block. I've heard mention of marking these with > eosin to make them easier to see. Has anyone done this? Or do you use > some other type of marking dye for this purpose? > Thanks for your input! > Rebecca > > Rebecca Ashley > Histotechnologist > Wyoming State Vet Lab > 1174 Snowy Range Rd. > Laramie, WY 82070 > Phone: 307-766-9946 > Fax: 307-721-2051 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 3 Date: Thu, 23 Jun 2016 19:04:44 +0000 From: Teri Johnson To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Shandon Varistain Gemini Slide Stainer Message-ID: Content-Type: text/plain; charset=WINDOWS-1252 Hi Thomas, Are you looking for true life expectancy or what is reported for depreciation? In my experience, most tissue processors never die, but only need to be retired due to lack of available support/parts or because a lab requires newer technology. Also in my experience, a "better" model comes out soon after I have purchased one. So the life span is probably 20 years after you wished you had a different one. :-) Best wishes, Teri Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com ------------------------------ Hi All, Does anyone know the life expectancy of the Gemini H&E stainer? My boss has asked me this question because we are in the process of requesting for a new one. Thomas Thomas Huynh BS, HT (ASCP) Histology Lab Supervisor |Department of Pathology HARRISHEALTH SYSTEM 5656 Kelly Street Houston, Tx 77026 Lyndon B. Johnson General Hospital (LBJGH) O: 713.566.5282 | F: 713.566.5285 | P: 713.297.1606 | Thomas.Huynh at harrishealth.org CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 4 Date: Thu, 23 Jun 2016 22:56:34 +0300 From: Jamal Rowaihi To: Mca Werdler , "Rebecca E. Ashley" Cc: ???? ??????? , "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Marking Tissues with Eosin Message-ID: Content-Type: text/plain; charset=utf-8 Hi?If you are using buffered Formaline so the Eosin color will not resist until the end of tissue processing.I recommend to add small amount of stock Eosin to the last alcohol in theTissue processor.? Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories?Sent from my cell phone -------- Original message --------From: Mca Werdler via Histonet Date: 6/23/16 8:49 PM (GMT+03:00) To: "Rebecca E. Ashley" Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Marking Tissues with Eosin Dear Rebecca, Yes this is possible. just don't use a too strong concentration. The eosin should give a slight pink color on the tissue after processing and after embedding. Good luck, Maarten 2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet < histonet at lists.utsouthwestern.edu>: > I had a biopsy today that was nearly impossible to see on the sponges > during embedding or in the block.? I've heard mention of marking these with > eosin to make them easier to see.? Has anyone done this?? Or do you use > some other type of marking dye for this purpose? > Thanks for your input! > Rebecca > > Rebecca Ashley > Histotechnologist > Wyoming State Vet Lab > 1174 Snowy Range Rd. > Laramie, WY 82070 > Phone: 307-766-9946 > Fax: 307-721-2051 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 23 Jun 2016 21:02:49 +0000 (UTC) From: Paula Keene Pierce To: Teri Johnson , Histonet Subject: Re: [Histonet] Shandon Varistain Gemini Slide Stainer Message-ID: <994846485.545115.1466715770021.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 This is so true! I had a Fisher Histomatic automated slide stainer that was manufactured in 1985 that I used until the building took a direct lightning strike JUST LAST YEAR! My old VIP is still going! ?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Teri Johnson via Histonet To: "histonet at lists.utsouthwestern.edu" Sent: Thursday, June 23, 2016 2:04 PM Subject: Re: [Histonet] Shandon Varistain Gemini Slide Stainer Hi Thomas, Are you looking for true life expectancy or what is reported for depreciation? In my experience, most tissue processors never die, but only need to be retired due to lack of available support/parts or because a lab requires newer technology. Also in my experience, a "better" model comes out soon after I have purchased one. So the life span is probably 20 years after you wished you had a different one. :-) Best wishes, Teri Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA? 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com ------------------------------ Hi All, Does anyone know the life expectancy of the Gemini H&E stainer? My boss has asked me this question because we are in the process of requesting for a new one. Thomas Thomas Huynh? BS, HT (ASCP) Histology Lab Supervisor |Department of Pathology HARRISHEALTH SYSTEM 5656 Kelly Street Houston, Tx 77026 Lyndon B. Johnson General Hospital (LBJGH) O: 713.566.5282 | F: 713.566.5285 | P: 713.297.1606 | Thomas.Huynh at harrishealth.org CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged.? This e-mail may also be confidential and/or privileged under Texas law.? The e-mail is for the use of only the individual or entity named above.? If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 23 Jun 2016 16:20:45 -0500 From: Cindy Bird To: Mca Werdler Cc: "Rebecca E. Ashley" , "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Marking Tissues with Eosin Message-ID: Content-Type: text/plain; charset="us-ascii" We place a small drop of concentrate straight on tissue. Sent from my iPhone > On Jun 23, 2016, at 12:56 PM, Mca Werdler via Histonet wrote: > > Dear Rebecca, > > Yes this is possible. just don't use a too strong concentration. The eosin > should give a slight pink color on the tissue after processing and after > embedding. > > Good luck, > > Maarten > > 2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet < > histonet at lists.utsouthwestern.edu>: > >> I had a biopsy today that was nearly impossible to see on the sponges >> during embedding or in the block. I've heard mention of marking these with >> eosin to make them easier to see. Has anyone done this? Or do you use >> some other type of marking dye for this purpose? >> Thanks for your input! >> Rebecca >> >> Rebecca Ashley >> Histotechnologist >> Wyoming State Vet Lab >> 1174 Snowy Range Rd. >> Laramie, WY 82070 >> Phone: 307-766-9946 >> Fax: 307-721-2051 >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 24 Jun 2016 13:19:43 +0000 From: Cynthia Robinson To: Cindy Bird , Mca Werdler Cc: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Marking Tissues with Eosin Message-ID: <4EE642D353925D4D96CB95E12427DBAE56DD4CF9 at NODCMSTMBX06.no.trinity-health.org> Content-Type: text/plain; charset="us-ascii" We use safranin at the grossing station and it is a dark pink at embedding. Works really well in our hands. Added plus is no fluorescent issues that you can have with eosin. Cindi ________________________________________ From: Cindy Bird via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, June 23, 2016 4:20 PM To: Mca Werdler Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Marking Tissues with Eosin We place a small drop of concentrate straight on tissue. Sent from my iPhone > On Jun 23, 2016, at 12:56 PM, Mca Werdler via Histonet wrote: > > Dear Rebecca, > > Yes this is possible. just don't use a too strong concentration. The eosin > should give a slight pink color on the tissue after processing and after > embedding. > > Good luck, > > Maarten > > 2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet < > histonet at lists.utsouthwestern.edu>: > >> I had a biopsy today that was nearly impossible to see on the sponges >> during embedding or in the block. I've heard mention of marking these with >> eosin to make them easier to see. Has anyone done this? Or do you use >> some other type of marking dye for this purpose? >> Thanks for your input! >> Rebecca >> >> Rebecca Ashley >> Histotechnologist >> Wyoming State Vet Lab >> 1174 Snowy Range Rd. >> Laramie, WY 82070 >> Phone: 307-766-9946 >> Fax: 307-721-2051 >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 151, Issue 22 ***************************************** From bakevictoria at gmail.com Fri Jun 24 17:15:31 2016 From: bakevictoria at gmail.com (Victoria Baker) Date: Fri, 24 Jun 2016 18:15:31 -0400 Subject: [Histonet] Dialyzed iron solution In-Reply-To: References: Message-ID: Hi. Back when I was in research we did Hale's Colloidal Iron stain. Making the dialyzed iron was really the big part and back then we were using a cellulose tubing that we had from another group. I've been trying to find the right type but there is just too many choices. I've also been trying to find a company that makes it as well. If anyone can forward some information on where I could find either of these, I would be grateful for it. Have a happy weekend! Vikki From PREISZNE at mail.etsu.edu Mon Jun 27 09:47:55 2016 From: PREISZNE at mail.etsu.edu (Preiszner, Johanna) Date: Mon, 27 Jun 2016 14:47:55 +0000 Subject: [Histonet] using FFPE positive control for frozen tissue IHC? Message-ID: Hi, We have difficulty obtaining frozen tissue to use as positive control when we do IHC on frozen sections... Is it allowed to use FFPE sections for this purpose? If not, can someone explain to me why not? Is it wrong by principle or prohibited by regulations? If it's the regulations, can someone give me a reference, please? I have not seen an antibody that works on FFPE tissue and refuses to work on frozen tissue...And the manufacturers always provide info about the type of tissue the antibody would work with. Thank you, Hanna Preiszner ETSU/QCOM From liz at premierlab.com Mon Jun 27 10:35:46 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Mon, 27 Jun 2016 09:35:46 -0600 Subject: [Histonet] using FFPE positive control for frozen tissue IHC? In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02BEC712800D@SBS2K8.premierlab.local> There are many companies that sell frozen tissue both normal and diseased. Frozen IHC does not normally require retrieval most FFPE IHC does. I don't think it would be good practice to do what you are suggesting. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Preiszner, Johanna via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, June 27, 2016 8:48 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] using FFPE positive control for frozen tissue IHC? Hi, We have difficulty obtaining frozen tissue to use as positive control when we do IHC on frozen sections... Is it allowed to use FFPE sections for this purpose? If not, can someone explain to me why not? Is it wrong by principle or prohibited by regulations? If it's the regulations, can someone give me a reference, please? I have not seen an antibody that works on FFPE tissue and refuses to work on frozen tissue...And the manufacturers always provide info about the type of tissue the antibody would work with. Thank you, Hanna Preiszner ETSU/QCOM _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ZDurkin at goodwin.edu Mon Jun 27 11:17:48 2016 From: ZDurkin at goodwin.edu (Zoe Durkin) Date: Mon, 27 Jun 2016 16:17:48 +0000 Subject: [Histonet] Part Time Histotech position in Richmond, VA Message-ID: <1467044268558.29366@goodwin.edu> Hello we are looking for a Histotechnician to work Part Time in Richmond, VA. This is a one person lab and we are looking for someone who is able to do all skills from start to finish. Job Summary: This position requires advanced technical and limited supervisory work in the preparation of tissue specimens for pathological examination. Work involves responsibility for accuracy in preparing, cutting, staining, and mounting tissue sections to assist pathologists in making correct diagnosis of a patient's disease. Assistance in training of laboratory aides and ensuring that work progresses in the absence of a supervisor. Work will be performed in accordance with standard laboratory practices and procedures. Work is performed under the supervision of the histology laboratory technical superior who reviews procedures and results. Duties and Responsibilities: * Receive tissue specimens from surgical and diagnostic cases * Accession of patient samples and maintain sample ordering in accordance with laboratory policies * Trim specimens into blocks and fix tissue in formalin or other fixing solutions, as appropriate * Tissue processing duties including fixation, dehydration, clearing, and paraffin infiltration of tissue samples * Tissue embedding * Operate and maintain all laboratory instrumentation in accordance with laboratory guidelines * Section tissue blocks for routine and special staining * Stain tissue samples using routine H&E and special staining procedures * Assist laboratory aides in selecting proper stains in order to bring out certain tissue elements as required for diagnosis * Inventory supplies and equipment and prepare requisition forms to replenish depleted supplies, as needed * Perform related work as required Education and Experience: * Minimum associates degree with coursework in biological sciences * At least 1 year experience in histological laboratory work * HT(ASCP) certification Knowledge, Skills, and Abilities: * Thorough knowledge of the principles and procedures of laboratory science. * Thorough knowledge of the principles and practices of histologic techniques. * Ability to perform assigned tasks according to exactly prescribed procedures and to make accurate observations of results. * Special ability required perceiving three-dimensional and geometrical relationships when cutting paraffin blocks and making embedding molds. * Ability to establish and maintain effective working relationships with physicians and fellow employees. * Skill in laboratory tissue handling techniques. Interested parties please email CV and/or resume to zdurkin at labpulsemed.com Regards, Zoe Ann Durkin, M.Ed., HT(ASCP) [https://webmail.goodwin.edu/owa/service.svc/s/GetFileAttachment?id=AAMkAGRhOWYxNzBmLWNjZjctNDVmYy05ZDk5LTdlZDNjMzE5NTJjYQBGAAAAAABUZnvWtsXpRJyVgt2mVB3wBwAJCa8KV29lRbm06b6LzgYLAAAAOJ6oAABFQ0bZLhXVSqffXA2yJJT1AAB2tpBiAAABEgAQAKTuwGqRD%2BVBoOJrtwqqFr8%3D&isImagePreview=True&X-OWA-CANARY=INMYuOOLeE6UEE152Tw3x-N_dTKRG9MI8Y7vbv1wTBvsam6-a6yPMENHZlKqx7pPlCgVB6ey-cE.] From emartinez2 at echd.org Tue Jun 28 14:58:15 2016 From: emartinez2 at echd.org (Estela Martinez) Date: Tue, 28 Jun 2016 19:58:15 +0000 Subject: [Histonet] Disposal of Histology Slides and Blocks Message-ID: Hello My Histo Friends, Has anyone heard if there is a difference on the disposal of blocks and slides of children under the age of 21? I have never heard of this before but I was asked today and was wondering. Estela Martinez CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party without permission of original user and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From mpence at grhs.net Tue Jun 28 15:10:52 2016 From: mpence at grhs.net (Mike Pence) Date: Tue, 28 Jun 2016 20:10:52 +0000 Subject: [Histonet] H Pylori control blocks Message-ID: Hey Histo world- Does anyone have any blocks to spare that are + for H Pylori? I could sure use a couple for IHC. Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 mpence at grhs.net | www.greatrivermedical.org. www.Facebook.com/GreatRiverHealthSystems | www.Twitter/GreatRiverMed Information in this communication, including attachments, is confidential and intended only for the addressee(s). This communication may contain privileged, confidential, proprietary or trade secret information entitled to protection or exemption from disclosure under law. If you are not an intended recipient, please know that any use, distribution or copying of this communication, or any action taken based on the information in this communication, is unauthorized and may be unlawful. If you received this communication in error, please notify the sender and delete this communication from your device. From Richard.Cartun at hhchealth.org Tue Jun 28 15:41:47 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 28 Jun 2016 20:41:47 +0000 Subject: [Histonet] Testicular biopsy for infertility Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E9535252E@HHCEXCHMB03.hhcsystem.org> What are people fixing testicular biopsies in to evaluate infertility? In the past, I believe fixatives such as Zenker's and Bouin's were used for this purpose since they enhance nuclear detail. Obviously, those fixatives can no longer be used. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From alamberth at lji.org Tue Jun 28 16:44:02 2016 From: alamberth at lji.org (Angela Lamberth) Date: Tue, 28 Jun 2016 14:44:02 -0700 Subject: [Histonet] =?utf-8?q?How_to_get_your_stains_more_vibrant_when_cut?= =?utf-8?q?ting_at_2=CE=BCm=3F?= Message-ID: When I cut at 2?m my H&Es and special stains look pale. How can I get my stains to pop or am I stuck with pale looking stains when sectioning that thin? I run manual specials and a manual regressive H&E. For H&E I've tried increasing my time in hematoxylin (beyond the manufacturer recommendation), diluting my acid alcohol differentiation, and increased time in eosin but the slides still lack the vibrancy that many of the postdocs desire. I use Shandon instant hematoxylin and alcoholic eosin by Thermo. Everything else I prepare in house from scratch. Any recommendations? From cmmathis at wakehealth.edu Wed Jun 29 08:16:16 2016 From: cmmathis at wakehealth.edu (Cathy M. Mathis/Comparative Medicine) Date: Wed, 29 Jun 2016 13:16:16 +0000 Subject: [Histonet] CD34 for primate tissue Message-ID: <4816F916B11AEC4E92A0D01372F573E2010FFC86E6@EXCHDB8.medctr.ad.wfubmc.edu> Good morning my fellow Histo-netters, Does anyone have experience with any CD34 antibody that would work in FFPE rhesus tissues? A few companies have one with clone QEBend 10 and say that it works but I have had no luck. I am using the Bond RX polymer system and I've tried all the epitope retrievals from Leica. Now I am try epitope retrieval offline; pronase, trypsin, pressure cooker with high and low pH solutions. Still no signal. Any help would be greatly appreciated. Cathy From gapollack at vassar.edu Wed Jun 29 09:50:05 2016 From: gapollack at vassar.edu (Gabrielle Pollack) Date: Wed, 29 Jun 2016 10:50:05 -0400 Subject: [Histonet] ARC immunohistochemistry- "sticky sections" in DAB Message-ID: To whom it may concern, I am a undergraduate researcher at Vassar College. I am performing immunohistochemistry on mice using Arc primary, anti-mouse biotinylated IgG (1:200),ABC (Vector labs) and DAB. So far we have gotten really great staining, however the last immuno I ran, the sections, once in DAB were sticking to the bottom of the wells. Does anyone have any idea as to why this would happen? Thank you! Best, Gabrielle From relia1 at earthlink.net Wed Jun 29 09:56:22 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 29 Jun 2016 10:56:22 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin - 6/29/2016 Sizzling new histology opportunities and Edible Firecrackers for your 4th of July celebration! Message-ID: <006e01d1d216$674f78b0$35ee6a10$@earthlink.net> Hi Histonetters! Have a safe and Happy July 4th Weekend. I Have A Fun Idea to Share for Pool Parties and Cookouts Sprinkle Red Pop Rocks And Blue Sprinkles On Iced Sugar Cookies To Make Edible Firecrackers!! I guarantee you will be the hit of your Independence Day Celebration! Do you have any fun ideas for the 4th to share? I have some exciting opportunities that I want to pass along as well. Maybe they will look good to you or someone you know. RELIA?S Hot Histology Opportunities!! IHC Tech Modesto, CA -Use your IHC Skills! Histotech Tyler, TX state of the art lab! Histotechnician Norfolk, VA $15K Sign-on bonus!!!! Lead Histotech Fayetteville, AR (run your own lab) Histotechnician Nashville, TN Asst. Supervisor Roswell, NM-Days!! Histology Tech Birmingham, AL days, dermpath Senior HTL Flagstaff, AZ -IHC/ISH Histotech Hammond, IN All of these jobs are full time permanent positions with some of the finest facilities in the country. My clients offer excellent compensation and benefits and most offer relocation assistance or sign- on bonuses. For more information for you or a friend please contact me toll free at the office at 866-607-3542 or on my cell call/text at 407-353-5070 or shoot me an email: relia1 at earthlink.net Have a Safe and Happy 4th of July!! Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From gayle.callis at bresnan.net Wed Jun 29 12:05:05 2016 From: gayle.callis at bresnan.net (Gayle Callis) Date: Wed, 29 Jun 2016 12:05:05 -0500 Subject: [Histonet] Bouins for testicular biopsies Message-ID: <000801d1d228$62a8ced0$27fa6c70$@bresnan.net> Richard, You wrote: What are people fixing testicular biopsies in to evaluate infertility? In the past, I believe fixatives such as Zenker's and Bouin's were used for this purpose since they enhance nuclear detail. Obviously, those fixatives can no longer be used. Thank you. *************************************************** I don't think Bouin's is forbidden in laboratories and we certainly used it routinely for the Masson Trichrome connective tissue stain. The problem is having stock Picric Acid in crystal form, now frowned upon by chemical safety people and eliminated from shelves these days. I had no problem storing stock picric acid under a layer of water and keeping crystals from outside edges of lid. Zenkers is obviously not used due to mercury content. Bouin's is still used for Masson's Trichrome staining and can be purchased ready-made from Sigma, Fisher and elsewhere. The key would be to use it to fix testicular biopsies, with no more than 72 hour fixation. Be careful to wipe any drips from around lids where picric acid crystals form, collect and dispose of this fixative per your lab's regulations. There is a B-5 substitute, sold by BBC, which may do the job just as well. This B-5 substitute is known to work well for bone marrow biopsies where good nuclear detail is important, and may be a good option. If your techs are using Bouins for Massons Trichrome connective tissue staining, you could get a small container for the biopsy. Good luck Gayle M. Callis HTL/HT/MT(ASCP) GCallis Histology Service LLC From gayle.callis at bresnan.net Wed Jun 29 12:23:16 2016 From: gayle.callis at bresnan.net (Gayle Callis) Date: Wed, 29 Jun 2016 12:23:16 -0500 Subject: [Histonet] 2 um sections H&E staining Message-ID: <001d01d1d22a$ecbe02f0$c63a08d0$@bresnan.net> You wrote: When I cut at 2?m my H&Es and special stains look pale. How can I get my stains to pop or am I stuck with pale looking stains when sectioning that thin? I run manual specials and a manual regressive H&E. For H&E I've tried increasing my time in hematoxylin (beyond the manufacturer recommendation), diluting my acid alcohol differentiation, and increased time in eosin but the slides still lack the vibrancy that many of the postdocs desire. I use Shandon instant hematoxylin and alcoholic eosin by Thermo. Everything else I prepare in house from scratch. Any recommendations? First is a question. Why do they require a 2?m thick section in the first place? I had a pathologist many years ago fall in love with these very thin section for all tissues with the same complaint of pale staining. It was explained to him that this thickness was excellent for bone marrow and renal biopsies but too thin for the majority of other tissues. Simple, you are slicing through cells much of the time and leaving only cell walls for staining. It there isn't enough thickness there, then hematoxylin doesn't have enough tissue thickness to be "vibrant", and the same for th eosin. The pathologist went back to the former routine 5?m thick sections. Some laboratories do use 4 ?m routinely. Things to try: 1. If this thickness is required to see basement membranes or marrow cells a. Do not use regressive hematoxylin and eosin, but rather progressive hematoxylin i.e. Gill III type formulation, and do NOT use any differentiation solution which can remove hematoxylin. b. Increase the thickness of section by trying 3 ?m and 4 ?m but use progressive H&E. If you don't need 2?m, then go to 4 or 5 c. Treat sections with 1% periodic acid for 10 min, rinse and then stain with progressive H&E. This technique is found in Sheehan and Hrapchak book. It might improve the staining with your sections. d. From rjbuesa at yahoo.com Wed Jun 29 12:27:33 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 29 Jun 2016 17:27:33 +0000 (UTC) Subject: [Histonet] =?utf-8?q?How_to_get_your_stains_more_vibrant_when_cut?= =?utf-8?q?ting_at_2=CE=BCm=3F?= In-Reply-To: References: Message-ID: <1054448194.965878.1467221253227.JavaMail.yahoo@mail.yahoo.com> Angela:"Pale" results are the trade-off for great quality very thin "2 ?m" sections but you can always improve intensity somewhat .1- your "regressive" stain, if it is "modern Harris" has the inherent problem of lacking mercury chloride and it is little you can do about. Perhaps if you use "progressive Mayer" you will get better results. You will not have to differentiate (with the intrinsic "danger" of leaving the section too pale) and if used fresh Mayer's can be a good approach.2- as to the counterstain perhaps you should add safranine to the eosin (20% safranine + 80% eosin) and will get a darker red.3- try to dehydrate as quickly as possible or even better, wash the sections in distilled water and place them in an oven at 60?C for 10 minutes and coverslip as usual. You will eliminate any "color wash" due to the alcohols. If you've not enough "trust" on dry/oven dehydration, try with some sections as a test. You will like the method.Ren? On Tuesday, June 28, 2016 5:53 PM, Angela Lamberth via Histonet wrote: When I cut at 2?m my H&Es and special stains look pale. How can I get my stains to pop or am I stuck with pale looking stains when sectioning that thin? I run manual specials and a manual regressive H&E. For H&E I've tried increasing my time in hematoxylin (beyond the manufacturer recommendation), diluting my acid alcohol differentiation, and increased time in eosin but the slides still lack the vibrancy that many of the postdocs desire. I use Shandon instant hematoxylin and alcoholic eosin by Thermo. Everything else I prepare in house from scratch. Any recommendations? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tejohnson at genoptix.com Wed Jun 29 12:36:07 2016 From: tejohnson at genoptix.com (Teri Johnson) Date: Wed, 29 Jun 2016 17:36:07 +0000 Subject: [Histonet] Testicular biopsy for infertility Message-ID: <7f06762323e249bb9ac9a9886944660d@PHUSCB-SP37MB01.genoptix.org> Dr. Rich Cartun asks: "What are people fixing testicular biopsies in to evaluate infertility? In the past, I believe fixatives such as Zenker's and Bouin's were used for this purpose since they enhance nuclear detail. Obviously, those fixatives can no longer be used. Thank you." I can understand keeping Zenker's out of the lab because of the mercury, but I'm at a loss why you cannot continue to use Bouins? You can buy the fixative fully prepared, or you can buy saturated aqueous picric acid from which you can prepare it in house. Doing it this way should keep the safety people calm since you are not storing "dry" chemical, which is the hazard. I suppose each state is different with regard to disposal, but I have always disposed Bouins as formaldehyde waste. Having said that, I would look for potential alternatives that contain acetic acid (since both fixatives use it) and might also consider a Zinc Formalin as a substitute for the mercury. So I'm thinking AZF (Acetic Zinc Formalin) or modified Carnoy (minus chloroform) might give you the nuclear morphology that is needed although both would still require some adjustment by the pathologists in recognizing their particular artifacts and how they can use it to aid the interpretation. Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From gayle.callis at bresnan.net Wed Jun 29 12:50:09 2016 From: gayle.callis at bresnan.net (Gayle Callis) Date: Wed, 29 Jun 2016 12:50:09 -0500 Subject: [Histonet] =?iso-8859-7?q?Re_=232=3A_=ECm__H=26E_staining=2C_WHOO?= =?iso-8859-7?q?PS!!?= Message-ID: <004201d1d22e$ae6f3fb0$0b4dbf10$@bresnan.net> Sorry about hitting send too soon. Repeat of things to try: a. Do not use regressive hematoxylin and eosin where hematoxylin can be overly differentiated i.e. removed from too thin sections. Use progressive hematoxylin i.e. Gill II or Gill III type formulation. DO NOT use acid alcohol differentiation with progressive hematoxylin. Try staining longer, i.e. 10 min in Gill III, and use acetic acid clarifier only 1 or 2 dips or skip clarifying solution entirely. b. Never use acid alcohol differentiation even with your hematoxylin c. Use progressive hematoxylin, and do not clarify or use acid alcohol differentiation solution. Wash well for 1 minute in running tap water then blue. d. Increase the thickness of sections to see if this satisfies the post-docs. Start with 3 ?m and 4 ?m but stain these sections with progressive H&E. If you don't need 2 ?m, then go to a more routine 4 ?m or 5 ?m thickness. You need to explain to these post docs about too thin sections do NOT have enough tissue/cell left to stain well enough. e. Treat sections with FRESH MADE 1% periodic acid for 10 min, rinse well and stain with progressive H&E. This periodic acid technique is found in Sheehan and Hrapchak Theory and Practice of Histotechnology book. PA treatment might improve the staining with your sections by making more groups on DNA available to hematoxylin. However, I didn't find it improved my thin section staining as much as I wanted. The sections were just too thin. f. Try Eosin-phloxine mixture, start dehydration in a few quick dips in 95%, then proceed to 100% alcohols. Eosin-phloxine is available as ready to use or make up in the lab. Sheehan and Hrapchak is also a source of this eosin formulation. Good luck Gayle M. Callis HT/HTL/MT(ASCP) GCallis Histology Service, LLC. From michang2014 at gmail.com Wed Jun 29 12:57:15 2016 From: michang2014 at gmail.com (Michelle Chang) Date: Wed, 29 Jun 2016 10:57:15 -0700 Subject: [Histonet] ARC immunohistochemistry- "sticky sections" in DAB Message-ID: Hi Gabrielle, Are you doing floating sections? Did you cut the tissue recently? Do you notice your tissue is also "sticky"? If you do, there is a possibility that the tissue may be infected by bacteria. Try washing the tissue with PBS or TBS with 0.1% sodium azide a couple time. Sometimes it help. Good Luck Best, Michelle From tkngflght at yahoo.com Wed Jun 29 14:10:33 2016 From: tkngflght at yahoo.com (Cheryl) Date: Wed, 29 Jun 2016 19:10:33 +0000 (UTC) Subject: [Histonet] Short term temp in Texas References: <1976979843.3771888.1467227433679.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1976979843.3771888.1467227433679.JavaMail.yahoo@mail.yahoo.com> Hi Histonetters -- I need a short term histologist (registered) for a three week stint in Texas.? GOOD lab, I'd go do it but the schedule doesn't line up for me :( Everything paid-- please respond via email with your resume.? Starts July 11-- Holler! admin at fullstaff.org Cheryl?Cheryl Kerry, HT(ASCP) Full Staff Inc. ? admin at fullstaff.org?800.756.3309 Phone & Fax https://www.facebook.com/TheHistologyCompany/ From Nancy_Schmitt at pa-ucl.com Wed Jun 29 14:30:08 2016 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Wed, 29 Jun 2016 19:30:08 +0000 Subject: [Histonet] HT grossing Message-ID: <055f4421e47040b9953c56dbfdf081bc@mercury.wad.pa-ucl.com> Hi All- I have a couple things I am looking for assist on today: 1. Are your HT's doing frozen section cutting? Are they also doing the frozen section gross and inking? Are they staining the slides? We are interested in doing this and looking for how others do. 2. Do your HT's gross smalls? What does that include? How did you train them and can you share that information? Is there a guide out there you would recommend? Appreciate your input, Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From edmartin26 at gmail.com Wed Jun 29 16:34:25 2016 From: edmartin26 at gmail.com (Eddie Martin) Date: Wed, 29 Jun 2016 16:34:25 -0500 Subject: [Histonet] CD34 for primate tissue In-Reply-To: <4816F916B11AEC4E92A0D01372F573E2010FFC86E6@EXCHDB8.medctr.ad.wfubmc.edu> References: <4816F916B11AEC4E92A0D01372F573E2010FFC86E6@EXCHDB8.medctr.ad.wfubmc.edu> Message-ID: <5FEA0959-4FB5-4DAA-A4FD-AD9ADF9C0D29@gmail.com> Hi Cathy, EDTA (Retrieval #2) for 20 minutes on the Bond-Rx should be sufficient. And all that is necessary. Please contact me if you need additional help. Best, Eddie Martin, HTL, QIHC Edmartin26 at gmail.com Sent from my iPhone > On Jun 29, 2016, at 8:16 AM, Cathy M. Mathis/Comparative Medicine wrote: > > Good morning my fellow Histo-netters, > Does anyone have experience with any CD34 antibody that would work in FFPE rhesus tissues? A few companies have one with clone QEBend 10 and say that it works but I have had no luck. I am using the Bond RX polymer system and I've tried all the epitope retrievals from Leica. Now I am try epitope retrieval offline; pronase, trypsin, pressure cooker with high and low pH solutions. Still no signal. Any help would be greatly appreciated. > Cathy > > > From cmmathis at wakehealth.edu Wed Jun 29 16:43:06 2016 From: cmmathis at wakehealth.edu (Cathy M. Mathis/Comparative Medicine) Date: Wed, 29 Jun 2016 21:43:06 +0000 Subject: [Histonet] CD34 for primate tissue In-Reply-To: <5FEA0959-4FB5-4DAA-A4FD-AD9ADF9C0D29@gmail.com> References: <4816F916B11AEC4E92A0D01372F573E2010FFC86E6@EXCHDB8.medctr.ad.wfubmc.edu> <5FEA0959-4FB5-4DAA-A4FD-AD9ADF9C0D29@gmail.com> Message-ID: <4816F916B11AEC4E92A0D01372F573E2010FFC8806@EXCHDB8.medctr.ad.wfubmc.edu> 2 more bits of information about my dilemma; I did try staining without any retrieval - no signal I am using rhesus kidney as a control (getting no signal), but I also ran some human kidney and got beautiful staining with a 20 minute HIER using a pH 8 EDTA buffer. So I know the antibodies and my protocols work, just not on my rhesus. Does anyone know of a CD34 that will work in this species? More suggestions please? Thank you all for your time. Cathy -----Original Message----- From: Eddie Martin [mailto:edmartin26 at gmail.com] Sent: Wednesday, June 29, 2016 5:34 PM To: Cathy M. Mathis/Comparative Medicine Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] CD34 for primate tissue Hi Cathy, EDTA (Retrieval #2) for 20 minutes on the Bond-Rx should be sufficient. And all that is necessary. Please contact me if you need additional help. Best, Eddie Martin, HTL, QIHC Edmartin26 at gmail.com Sent from my iPhone > On Jun 29, 2016, at 8:16 AM, Cathy M. Mathis/Comparative Medicine wrote: > > Good morning my fellow Histo-netters, > Does anyone have experience with any CD34 antibody that would work in FFPE rhesus tissues? A few companies have one with clone QEBend 10 and say that it works but I have had no luck. I am using the Bond RX polymer system and I've tried all the epitope retrievals from Leica. Now I am try epitope retrieval offline; pronase, trypsin, pressure cooker with high and low pH solutions. Still no signal. Any help would be greatly appreciated. > Cathy > > > From garethdavisyuma at gmail.com Wed Jun 29 18:17:38 2016 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Wed, 29 Jun 2016 16:17:38 -0700 Subject: [Histonet] Transport training for formalin fixed specimens Message-ID: Okay, is there a training course for just transportion formalin fixed specimens. CAP told me last year that anyone picking up specimens for us has to go through a training course. The inspector seemed to be more concerned with the employees knowledge of formalin and possible spills. So, does anyone know what course I am suppose to be using? I have looked on the CDC website, but all the courses I find refer to specific infectious disease. Thanks in advance. -- Gareth B. Davis, HT (ASCPcm), QIHC Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From tony.henwood at health.nsw.gov.au Wed Jun 29 18:58:31 2016 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 29 Jun 2016 23:58:31 +0000 Subject: [Histonet] Transport training for formalin fixed specimens In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF014A52BC@SVDCMBX-MEX008.nswhealth.net> Hi Gareth, You will probably have to design your own Continuing Education Session. I would recommend the following topics: 1. Nature of formaldehyde - safety aspects, explanation of the MSDS. 2. What formaldehyde is used for 3. Appropriate packaging of pots containing specimens in formalin 4. Spill containment, neutralisation, clean-up procedures and Notification requirements. Probably only take 30 minutes or so. Issue each attendee with a certificate and record attendees for CAP. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Gareth Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 30 June 2016 9:18 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Transport training for formalin fixed specimens Okay, is there a training course for just transportion formalin fixed specimens. CAP told me last year that anyone picking up specimens for us has to go through a training course. The inspector seemed to be more concerned with the employees knowledge of formalin and possible spills. So, does anyone know what course I am suppose to be using? I have looked on the CDC website, but all the courses I find refer to specific infectious disease. Thanks in advance. -- Gareth B. Davis, HT (ASCPcm), QIHC Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From liz at premierlab.com Wed Jun 29 19:18:31 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Wed, 29 Jun 2016 18:18:31 -0600 Subject: [Histonet] Transport training for formalin fixed specimens In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02BEC712806D@SBS2K8.premierlab.local> Gareth I would think it would have more to do with OSHA than CDC - this could involve several modules. We have modules that cover the following - this is not all that we train on. If you are a small business OSHA can help out. We have utilized their services with a voluntary compliance audit. We were referred to the Chemical Hygiene Officer at the local university and they helped us out. * Formaldehyde Awareness * General Safety Awareness * Hazard Communication * Laboratory & Chemical Hygiene Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Gareth Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, June 29, 2016 5:18 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Transport training for formalin fixed specimens Okay, is there a training course for just transportion formalin fixed specimens. CAP told me last year that anyone picking up specimens for us has to go through a training course. The inspector seemed to be more concerned with the employees knowledge of formalin and possible spills. So, does anyone know what course I am suppose to be using? I have looked on the CDC website, but all the courses I find refer to specific infectious disease. Thanks in advance. -- Gareth B. Davis, HT (ASCPcm), QIHC Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From edmartin26 at gmail.com Wed Jun 29 19:22:25 2016 From: edmartin26 at gmail.com (Eddie Martin) Date: Wed, 29 Jun 2016 19:22:25 -0500 Subject: [Histonet] CD34 for primate tissue In-Reply-To: <4816F916B11AEC4E92A0D01372F573E2010FFC8806@EXCHDB8.medctr.ad.wfubmc.edu> References: <4816F916B11AEC4E92A0D01372F573E2010FFC86E6@EXCHDB8.medctr.ad.wfubmc.edu> <5FEA0959-4FB5-4DAA-A4FD-AD9ADF9C0D29@gmail.com> <4816F916B11AEC4E92A0D01372F573E2010FFC8806@EXCHDB8.medctr.ad.wfubmc.edu> Message-ID: <35365E30-12FB-43A4-A604-788A3C337314@gmail.com> What species is the kidney you are trying to stain with IHC. Because you mentioned that you used normal human kidney and it worked , but Rhesus kidney didn't stain. I'm wondering if the Rhesus kidney is human or another animal species? The Novocastra CD34 (QBEND10) is intended for human tissue. Best, Eddie Martin, HTL,QIHC Sent from my iPhone > On Jun 29, 2016, at 4:43 PM, Cathy M. Mathis/Comparative Medicine wrote: > > 2 more bits of information about my dilemma; > I did try staining without any retrieval - no signal > I am using rhesus kidney as a control (getting no signal), but I also ran some human kidney and got beautiful staining with a 20 minute HIER using a pH 8 EDTA buffer. > So I know the antibodies and my protocols work, just not on my rhesus. Does anyone know of a CD34 that will work in this species? > More suggestions please? Thank you all for your time. > Cathy > > -----Original Message----- > From: Eddie Martin [mailto:edmartin26 at gmail.com] > Sent: Wednesday, June 29, 2016 5:34 PM > To: Cathy M. Mathis/Comparative Medicine > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] CD34 for primate tissue > > Hi Cathy, > > EDTA (Retrieval #2) for 20 minutes on the Bond-Rx should be sufficient. And all that is necessary. Please contact me if you need additional help. > > Best, > Eddie Martin, HTL, QIHC > Edmartin26 at gmail.com > > Sent from my iPhone > >> On Jun 29, 2016, at 8:16 AM, Cathy M. Mathis/Comparative Medicine wrote: >> >> Good morning my fellow Histo-netters, >> Does anyone have experience with any CD34 antibody that would work in FFPE rhesus tissues? A few companies have one with clone QEBend 10 and say that it works but I have had no luck. I am using the Bond RX polymer system and I've tried all the epitope retrievals from Leica. Now I am try epitope retrieval offline; pronase, trypsin, pressure cooker with high and low pH solutions. Still no signal. Any help would be greatly appreciated. >> Cathy > From LaurenHegnerSweeney at uga.edu Thu Jun 30 07:22:04 2016 From: LaurenHegnerSweeney at uga.edu (Lauren Sweeney) Date: Thu, 30 Jun 2016 12:22:04 +0000 Subject: [Histonet] Trichrome Message-ID: Hello all, I was wondering if anyone has any experience with doing a trichrome and leaving it to mordant in Bouin's for longer than 24 hours. Does this overstain and ruin the stain? Thanks, L From DEBORAH_ELLENBURG at bshsi.org Thu Jun 30 08:46:28 2016 From: DEBORAH_ELLENBURG at bshsi.org (Ellenburg, Deborah) Date: Thu, 30 Jun 2016 13:46:28 +0000 Subject: [Histonet] UNSUBSCRIBE Message-ID: Thank you Deborah Ellenburg, HT (ASCP) Clinical Lead St. Francis Health System One St. Francis Drive Greenville, SC 29601 Office Phone: 864-255-1582 Cell Phone: 864-444-8291 FAX (864) 679-8963 "This communication may contain CONFIDENTIAL and PRIVILEGED information for the sole use of the intended recipient(s). If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender by reply e-mail or telephone (864-255-1670) and delete all copies of this message." ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From cmmathis at wakehealth.edu Thu Jun 30 09:06:30 2016 From: cmmathis at wakehealth.edu (Cathy M. Mathis/Comparative Medicine) Date: Thu, 30 Jun 2016 14:06:30 +0000 Subject: [Histonet] CD34 for primate tissue In-Reply-To: <35365E30-12FB-43A4-A604-788A3C337314@gmail.com> References: <4816F916B11AEC4E92A0D01372F573E2010FFC86E6@EXCHDB8.medctr.ad.wfubmc.edu> <5FEA0959-4FB5-4DAA-A4FD-AD9ADF9C0D29@gmail.com> <4816F916B11AEC4E92A0D01372F573E2010FFC8806@EXCHDB8.medctr.ad.wfubmc.edu> <35365E30-12FB-43A4-A604-788A3C337314@gmail.com> Message-ID: <4816F916B11AEC4E92A0D01372F573E2010FFC88E6@EXCHDB8.medctr.ad.wfubmc.edu> Rhesus is a species of old world monkey. There are a couple of other companies that have this same clone and on their datasheets they say it cross reacts with rhesus monkey. The CD34s (2) that I have do not state on their data sheets that they cross react with anything except human and yet are the same clone as the companies that say it does. I guess I will just break down and purchase a different clone that states it works in rhesus monkey. Cathy -----Original Message----- From: Eddie Martin [mailto:edmartin26 at gmail.com] Sent: Wednesday, June 29, 2016 8:22 PM To: Cathy M. Mathis/Comparative Medicine Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] CD34 for primate tissue What species is the kidney you are trying to stain with IHC. Because you mentioned that you used normal human kidney and it worked , but Rhesus kidney didn't stain. I'm wondering if the Rhesus kidney is human or another animal species? The Novocastra CD34 (QBEND10) is intended for human tissue. Best, Eddie Martin, HTL,QIHC Sent from my iPhone > On Jun 29, 2016, at 4:43 PM, Cathy M. Mathis/Comparative Medicine wrote: > > 2 more bits of information about my dilemma; I did try staining > without any retrieval - no signal I am using rhesus kidney as a > control (getting no signal), but I also ran some human kidney and got beautiful staining with a 20 minute HIER using a pH 8 EDTA buffer. > So I know the antibodies and my protocols work, just not on my rhesus. Does anyone know of a CD34 that will work in this species? > More suggestions please? Thank you all for your time. > Cathy > > -----Original Message----- > From: Eddie Martin [mailto:edmartin26 at gmail.com] > Sent: Wednesday, June 29, 2016 5:34 PM > To: Cathy M. Mathis/Comparative Medicine > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] CD34 for primate tissue > > Hi Cathy, > > EDTA (Retrieval #2) for 20 minutes on the Bond-Rx should be sufficient. And all that is necessary. Please contact me if you need additional help. > > Best, > Eddie Martin, HTL, QIHC > Edmartin26 at gmail.com > > Sent from my iPhone > >> On Jun 29, 2016, at 8:16 AM, Cathy M. Mathis/Comparative Medicine wrote: >> >> Good morning my fellow Histo-netters, Does anyone have experience >> with any CD34 antibody that would work in FFPE rhesus tissues? A few companies have one with clone QEBend 10 and say that it works but I have had no luck. I am using the Bond RX polymer system and I've tried all the epitope retrievals from Leica. Now I am try epitope retrieval offline; pronase, trypsin, pressure cooker with high and low pH solutions. Still no signal. Any help would be greatly appreciated. >> Cathy > From overn003 at umn.edu Thu Jun 30 09:33:40 2016 From: overn003 at umn.edu (Paula Overn) Date: Thu, 30 Jun 2016 09:33:40 -0500 Subject: [Histonet] IHC staining on tissues placed in Kerasoft Message-ID: Hello all, Our lab uses Kerasoft to soften our nails and nail bed tumors. We recently had a case where every IHC stain was negative (including internal positive) with the exception of cytokeratin AE1/3. Has anyone else experienced this? Does anyone have a better solution to soften nails when IHC staining might be needed? Thanks! Paula Overn 651-254-9652 Email: overn003 at umn.edu From tbraud at holyredeemer.com Thu Jun 30 09:33:25 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 30 Jun 2016 14:33:25 +0000 Subject: [Histonet] Testicular biopsies Message-ID: <48E053DDF6CE074DB6A7414BA05403F807DDF4@HRHEX02-HOS.holyredeemer.local> I must be missing the obvious. Why can one no longer use Bouins? We still use Bouins. Terri L. Braud, HT(ASCP) 3. Testicular biopsy for infertility (Cartun, Richard) Message: 3 Date: Tue, 28 Jun 2016 20:41:47 +0000 From: "Cartun, Richard" What are people fixing testicular biopsies in to evaluate infertility? In the past, I believe fixatives such as Zenker's and Bouin's were used for this purpose since they enhance nuclear detail. Obviously, those fixatives can no longer be used. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax From edmartin26 at gmail.com Thu Jun 30 09:49:58 2016 From: edmartin26 at gmail.com (Eddie Martin) Date: Thu, 30 Jun 2016 09:49:58 -0500 Subject: [Histonet] CD34 for primate tissue In-Reply-To: <4816F916B11AEC4E92A0D01372F573E2010FFC88E6@EXCHDB8.medctr.ad.wfubmc.edu> References: <4816F916B11AEC4E92A0D01372F573E2010FFC86E6@EXCHDB8.medctr.ad.wfubmc.edu> <5FEA0959-4FB5-4DAA-A4FD-AD9ADF9C0D29@gmail.com> <4816F916B11AEC4E92A0D01372F573E2010FFC8806@EXCHDB8.medctr.ad.wfubmc.edu> <35365E30-12FB-43A4-A604-788A3C337314@gmail.com> <4816F916B11AEC4E92A0D01372F573E2010FFC88E6@EXCHDB8.medctr.ad.wfubmc.edu> Message-ID: Hi Cathy, I haven't heard of the cross reactivity with monkey species using Novocastra CD34. However, novocastra's antibody is a monoclonal antibody. You may get better staining utilizing a polyclonal CD34 instead. Thermo-Fisher and Abcam provide a polyclonal CD34. If you reach out to them, they may be able to provide you with a sample size 0.1 microliter if they have them available. I hope his helps! Best, Eddie Sent from my iPhone > On Jun 30, 2016, at 9:06 AM, Cathy M. Mathis/Comparative Medicine wrote: > > Rhesus is a species of old world monkey. There are a couple of other companies that have this same clone and on their datasheets they say it cross reacts with rhesus monkey. The CD34s (2) that I have do not state on their data sheets that they cross react with anything except human and yet are the same clone as the companies that say it does. I guess I will just break down and purchase a different clone that states it works in rhesus monkey. > Cathy > > -----Original Message----- > From: Eddie Martin [mailto:edmartin26 at gmail.com] > Sent: Wednesday, June 29, 2016 8:22 PM > To: Cathy M. Mathis/Comparative Medicine > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] CD34 for primate tissue > > What species is the kidney you are trying to stain with IHC. Because you mentioned that you used normal human kidney and it worked , but Rhesus kidney didn't stain. I'm wondering if the Rhesus kidney is human or another animal species? The Novocastra CD34 (QBEND10) is intended for human tissue. > > Best, > Eddie Martin, HTL,QIHC > > Sent from my iPhone > >> On Jun 29, 2016, at 4:43 PM, Cathy M. Mathis/Comparative Medicine wrote: >> >> 2 more bits of information about my dilemma; I did try staining >> without any retrieval - no signal I am using rhesus kidney as a >> control (getting no signal), but I also ran some human kidney and got beautiful staining with a 20 minute HIER using a pH 8 EDTA buffer. >> So I know the antibodies and my protocols work, just not on my rhesus. Does anyone know of a CD34 that will work in this species? >> More suggestions please? Thank you all for your time. >> Cathy >> >> -----Original Message----- >> From: Eddie Martin [mailto:edmartin26 at gmail.com] >> Sent: Wednesday, June 29, 2016 5:34 PM >> To: Cathy M. Mathis/Comparative Medicine >> Cc: histonet at lists.utsouthwestern.edu >> Subject: Re: [Histonet] CD34 for primate tissue >> >> Hi Cathy, >> >> EDTA (Retrieval #2) for 20 minutes on the Bond-Rx should be sufficient. And all that is necessary. Please contact me if you need additional help. >> >> Best, >> Eddie Martin, HTL, QIHC >> Edmartin26 at gmail.com >> >> Sent from my iPhone >> >>> On Jun 29, 2016, at 8:16 AM, Cathy M. Mathis/Comparative Medicine wrote: >>> >>> Good morning my fellow Histo-netters, Does anyone have experience >>> with any CD34 antibody that would work in FFPE rhesus tissues? A few companies have one with clone QEBend 10 and say that it works but I have had no luck. I am using the Bond RX polymer system and I've tried all the epitope retrievals from Leica. Now I am try epitope retrieval offline; pronase, trypsin, pressure cooker with high and low pH solutions. Still no signal. Any help would be greatly appreciated. >>> Cathy >> > > > From liz at premierlab.com Thu Jun 30 10:25:08 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Thu, 30 Jun 2016 09:25:08 -0600 Subject: [Histonet] CD34 for primate tissue In-Reply-To: <4816F916B11AEC4E92A0D01372F573E2010FFC88E6@EXCHDB8.medctr.ad.wfubmc.edu> References: <4816F916B11AEC4E92A0D01372F573E2010FFC86E6@EXCHDB8.medctr.ad.wfubmc.edu> <5FEA0959-4FB5-4DAA-A4FD-AD9ADF9C0D29@gmail.com> <4816F916B11AEC4E92A0D01372F573E2010FFC8806@EXCHDB8.medctr.ad.wfubmc.edu> <35365E30-12FB-43A4-A604-788A3C337314@gmail.com> <4816F916B11AEC4E92A0D01372F573E2010FFC88E6@EXCHDB8.medctr.ad.wfubmc.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE02BEC712806E@SBS2K8.premierlab.local> Cathy Before doing that I would check the sequence homology of the immunogen between human and rhesus. I would do this for all sources of the antibody, if the company does not want to provide you with the sequence then technical support should be able to blast the sequence for you and get back to you the sequence homology, greater than 70% is worth a shot it may not work or it may not. You also need to check if it's the same clone it might be the same antibody just distributed by different vendors, check the protein concentration, purification method, isotype, and if there are matching images you are likely looking at the same antibody so you don't want to waste your time with ordering the same antibody but from a different vendor. Remember information on specification sheets can be inaccurate - this depends upon the vendor, some vendors actually test internally while other vendors rely of user for cross reactivity information so you need to take that information with a grain of salt. My suggestion is to take a look at the antibody on CiteAb - this website is a bit better than biocompare since it rates antibodies by number of citations, check to see if the other antibodies have publications with your use case. The link for CiteAb is https://www.citeab.com/ We find with some antibodies that work across species that the primary antibody concentration may fluctuate so a concentration that works in human may not work in the particular species you are working with. There are many other parameters such as time in fixation between your human tissue and rhesus tissue. Are you getting any hint of staining or is it just completely negative. It's really important to spend some significant time upfront when researching antibodies for a new target - you want to biases yourself towards success. If you are an NSH member I posted a worksheet for target development on the BLOCK you can access it there. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Cathy M. Mathis/Comparative Medicine via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, June 30, 2016 8:07 AM To: histonet at lists.utsouthwestern.edu Cc: Eddie Martin Subject: Re: [Histonet] CD34 for primate tissue Rhesus is a species of old world monkey. There are a couple of other companies that have this same clone and on their datasheets they say it cross reacts with rhesus monkey. The CD34s (2) that I have do not state on their data sheets that they cross react with anything except human and yet are the same clone as the companies that say it does. I guess I will just break down and purchase a different clone that states it works in rhesus monkey. Cathy -----Original Message----- From: Eddie Martin [mailto:edmartin26 at gmail.com] Sent: Wednesday, June 29, 2016 8:22 PM To: Cathy M. Mathis/Comparative Medicine Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] CD34 for primate tissue What species is the kidney you are trying to stain with IHC. Because you mentioned that you used normal human kidney and it worked , but Rhesus kidney didn't stain. I'm wondering if the Rhesus kidney is human or another animal species? The Novocastra CD34 (QBEND10) is intended for human tissue. Best, Eddie Martin, HTL,QIHC Sent from my iPhone > On Jun 29, 2016, at 4:43 PM, Cathy M. Mathis/Comparative Medicine wrote: > > 2 more bits of information about my dilemma; I did try staining > without any retrieval - no signal I am using rhesus kidney as a > control (getting no signal), but I also ran some human kidney and got beautiful staining with a 20 minute HIER using a pH 8 EDTA buffer. > So I know the antibodies and my protocols work, just not on my rhesus. Does anyone know of a CD34 that will work in this species? > More suggestions please? Thank you all for your time. > Cathy > > -----Original Message----- > From: Eddie Martin [mailto:edmartin26 at gmail.com] > Sent: Wednesday, June 29, 2016 5:34 PM > To: Cathy M. Mathis/Comparative Medicine > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] CD34 for primate tissue > > Hi Cathy, > > EDTA (Retrieval #2) for 20 minutes on the Bond-Rx should be sufficient. And all that is necessary. Please contact me if you need additional help. > > Best, > Eddie Martin, HTL, QIHC > Edmartin26 at gmail.com > > Sent from my iPhone > >> On Jun 29, 2016, at 8:16 AM, Cathy M. Mathis/Comparative Medicine wrote: >> >> Good morning my fellow Histo-netters, Does anyone have experience >> with any CD34 antibody that would work in FFPE rhesus tissues? A few companies have one with clone QEBend 10 and say that it works but I have had no luck. I am using the Bond RX polymer system and I've tried all the epitope retrievals from Leica. Now I am try epitope retrieval offline; pronase, trypsin, pressure cooker with high and low pH solutions. Still no signal. Any help would be greatly appreciated. >> Cathy > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mfb.encarnacion at gmail.com Thu Jun 30 11:06:46 2016 From: mfb.encarnacion at gmail.com (Mary Faith Encarnacion) Date: Thu, 30 Jun 2016 09:06:46 -0700 Subject: [Histonet] Embedding Centers - HistoStar vs. TEC 5? Message-ID: Hello Histonetters! My lab is on the hunt for a new embedding center. We've demo-ed the Leica Arcadia, the Thermo HistoStar, and the Sakura TEC 5. The votes are split between the HistoStar and TEC 5. I'd like to know anyone's thoughts on these two instruments...pros, cons, your experiences. Are there any more that we should try out? Thanks so much! Can't wait to hear your thoughts. Best, Mary Faith Encarnacion, MHA, HTL(ASCP)QIHC Supervisory Histopathology Technician Pathology and Lab Medicine Service VA Palo Alto Health Care System Phone: (650) 493-5000 x67327 (office); x65086 (lab) From rsrichmond at gmail.com Thu Jun 30 11:30:04 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 30 Jun 2016 12:30:04 -0400 Subject: [Histonet] Bouins for testicular biopsies Message-ID: Richard W. Cartun, MS, PhD in Hartford CT asks: >>What are people fixing testicular biopsies in to evaluate infertility? In the past, I believe fixatives such as Zenker's and Bouin's were used for this purpose since they enhance nuclear detail. Obviously, those fixatives can no longer be used.<< I don't think Bouin's fixative has been entirely banned, but it's a nuisance - the picric acid stains almost anything it gets spilled on, and there are the regulatory issues. I've had acceptable results with strongly acid fixatives, mostly with Davidson's fixative (3 parts water, 3 parts reagent alcohol, 2 parts 37% formaldehyde, 1 part glacial acetic acid). I'd want to try it out on a non-critical specimen - either orchiectomy tissue or animal material - before inflicting it on a real patient. I suppose a spay-neuter clinic could help. Bob Richmond Samurai Pathologist Maryville TN From alamberth at lji.org Thu Jun 30 12:41:38 2016 From: alamberth at lji.org (Angela Lamberth) Date: Thu, 30 Jun 2016 10:41:38 -0700 Subject: [Histonet] =?utf-8?q?How_to_get_your_stains_more_vibrant_when_cut?= =?utf-8?q?ting_at_2=CE=BCm=3F?= In-Reply-To: <1054448194.965878.1467221253227.JavaMail.yahoo@mail.yahoo.com> References: <1054448194.965878.1467221253227.JavaMail.yahoo@mail.yahoo.com> Message-ID: Thank you all very much for your suggestions. I'm going to play around with a progressive H&E when I return from vacation next month. I do have safranin on hand but will need to order some phloxine to experiment with. I will probably need to order some additional supplies to make the hematoxylin. I'm not sure yet if I'll start with Mayer's or Gill's or maybe even Erlich's. I should note that cutting at 2 isn't required, but it is desired once they saw that I can. And since I can, I aim to please! :-) In addition, I'm looking forward to trying the oven dry method before coverslipping. A rapid dehydration isn't really possible since I'm working with a xylene substitute (Pro-Par) and have been battling eosin carryover but that is a whole different animal for another thread. Thanks again! I'll report back in a month or 2. On Wed, Jun 29, 2016 at 10:27 AM, Rene J Buesa wrote: > > Angela: > "Pale" results are the trade-off for great quality very thin "2 ?m" > sections but you can always improve intensity somewhat . > 1- your "regressive" stain, if it is "modern Harris" has the inherent > problem of lacking mercury chloride and it is little you can do about. > Perhaps if you use "progressive Mayer" you will get better results. You > will not have to differentiate (with the intrinsic "danger" of leaving the > section too pale) and if used fresh Mayer's can be a good approach. > 2- as to the counterstain perhaps you should add safranine to the eosin > (20% safranine + 80% eosin) and will get a darker red. > 3- try to dehydrate as quickly as possible or even better, wash the > sections in distilled water and place them in an oven at 60?C for 10 > minutes and coverslip as usual. You will eliminate any "color wash" due to > the alcohols. > If you've not enough "trust" on dry/oven dehydration, try with some > sections as a test. You will like the method. > Ren? > > > On Tuesday, June 28, 2016 5:53 PM, Angela Lamberth via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > When I cut at 2?m my H&Es and special stains look pale. How can I get my > stains to pop or am I stuck with pale looking stains when sectioning that > thin? > > I run manual specials and a manual regressive H&E. For H&E I've tried > increasing my time in hematoxylin (beyond the manufacturer recommendation), > diluting my acid alcohol differentiation, and increased time in eosin but > the slides still lack the vibrancy that many of the postdocs desire. > > I use Shandon instant hematoxylin and alcoholic eosin by Thermo. Everything > else I prepare in house from scratch. Any recommendations? > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- Angela Lamberth Histology Technician II Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 From denise.croix at roche.com Thu Jun 30 16:57:53 2016 From: denise.croix at roche.com (Croix, Denise) Date: Thu, 30 Jun 2016 16:57:53 -0500 Subject: [Histonet] CD34 for primate tissue Message-ID: Hi Cathy, When I was doing work with NHP tissue (both flow cytometry and IHC) there were several sources that I utilized for info on antibodies that were cross-reactivity with various NHP species. The NIH hosts a NHP reagent resource website (nhpreagents.org) that shows which commercially available antibodies cross react with non-human primate tissues. They note that there are discrepancies reported about cross-reactivity with the QBEND10 antibody for rhesus and possibly cynos. You will need to still check with the vendors to confirm if the antibody has been tested in FFPE specimens as that is not indicated on the site but it should give you a starting point to work from. I've had personal experience with cyno & rhesus T cells where individual monkeys would not react with certain anti-human antibodies. For example, I had a couple of research animals that were negative for a CD3 antibody but yet they stained with CD4 and CD8 antibodies. Good luck! Denise -- [image: Powered by Sigstr] *Denise A Croix, Ph.D.* Pathology Solutions Specialist - PD-L1 SP142 Assay Roche Diagnostics Corporation Tissue Diagnostics Division 972-207-7305 denise.croix at roche.com www.ventana.com [image: Powered by Sigstr] [image: Powered by Sigstr]