From LRaff at uropartners.com Fri Jul 1 08:44:10 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 1 Jul 2016 13:44:10 +0000 Subject: [Histonet] Just some before the long weekend Friday Fun-lab related blog Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10E5E6FC@COLOEXCH01.uropartners.local> Pathologists couldn't do it without great histology teams, so thanks and have a Happy 4th. http://www.chicagonow.com/downsize-maybe/2016/06/reading-a-prostate-biopsy-inside-the-mind-of-a-pathologist/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From abadesuyi at nrh-ok.com Fri Jul 1 09:45:31 2016 From: abadesuyi at nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Fri, 1 Jul 2016 09:45:31 -0500 Subject: [Histonet] Certification Message-ID: <04EE4F75BB5FB246ADB68D69B7460443AF7A2CEA42@MAIL.nrhnt.nrh-ok.com> Hi, I hope you guys are doing great. Please I have a question and would appreciate your contributions. I have a tech that could not pass the certification test, when the ASCP were still accepting high school diploma for HT. The tech is no longer eligible to take the test again, because he did not have the minimum requirement (i.e. Associate Degree) to register for the test. He wants me to promote him to Histology Lead Tech/Histology Coordinator. What do you guys think about this? Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 Cell: 405-973-6363 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From tbraud at holyredeemer.com Fri Jul 1 12:21:58 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 1 Jul 2016 17:21:58 +0000 Subject: [Histonet] Certification Message-ID: <48E053DDF6CE074DB6A7414BA05403F807E337@HRHEX02-HOS.holyredeemer.local> Respectfully, a public forum is no place to discuss personnel issues on such a personal level. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 4. Certification (Adesupo, Adesuyi (Banjo)) ------------------------------ Message: 4 Date: Fri, 1 Jul 2016 09:45:31 -0500 From: "Adesupo, Adesuyi (Banjo)" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Certification Hi, I hope you guys are doing great. Please I have a question and would appreciate your contributions. I have a tech that could not pass the certification test, when the ASCP were still accepting high school diploma for HT. The tech is no longer eligible to take the test again, because he did not have the minimum requirement (i.e. Associate Degree) to register for the test. He wants me to promote him to Histology Lead Tech/Histology Coordinator. What do you guys think about this? Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 Cell: 405-973-6363 ===================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 152, Issue 1 **************************************** From rjbuesa at yahoo.com Fri Jul 1 13:48:38 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 1 Jul 2016 18:48:38 +0000 (UTC) Subject: [Histonet] Certification In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F807E337@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F807E337@HRHEX02-HOS.holyredeemer.local> Message-ID: <121392237.2016074.1467398918399.JavaMail.yahoo@mail.yahoo.com> Your tech has an "above deserved" expectation.How would you even consider promoting somebody who is not qualified to even be HT certified to Lead HT/Coordinator?This is disrespectful for those who reversing that position have been unable to achieve it.It speaks volumes about your tech aspirations and your managerial skills.Ren? On Friday, July 1, 2016 1:50 PM, Terri Braud via Histonet wrote: Respectfully, a public forum is no place to discuss? personnel issues on such a personal level.? Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 ? 4. Certification (Adesupo, Adesuyi (Banjo)) ------------------------------ Message: 4 Date: Fri, 1 Jul 2016 09:45:31 -0500 From: "Adesupo, Adesuyi (Banjo)" To: "'histonet at lists.utsouthwestern.edu'" ??? Subject: [Histonet] Certification Hi, ? ? I hope you guys are doing great. Please I have a question and would appreciate your contributions. I have a tech that could not pass the certification test, when the ASCP were still accepting high school diploma for HT. The tech is no longer eligible to take the test again, because he did not have the minimum requirement (i.e. Associate Degree) to register for the test. He wants me to promote him to Histology Lead Tech/Histology Coordinator. What do you guys think about this? Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 Cell: 405-973-6363 ===================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 152, Issue 1 **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stacy_McLaughlin at cooley-dickinson.org Fri Jul 1 14:09:37 2016 From: Stacy_McLaughlin at cooley-dickinson.org (Stacy McLaughlin) Date: Fri, 1 Jul 2016 19:09:37 +0000 Subject: [Histonet] [BULK] Re: Certification In-Reply-To: <121392237.2016074.1467398918399.JavaMail.yahoo@mail.yahoo.com> References: <48E053DDF6CE074DB6A7414BA05403F807E337@HRHEX02-HOS.holyredeemer.local> <121392237.2016074.1467398918399.JavaMail.yahoo@mail.yahoo.com> Message-ID: I will respectfully comment as well. A public forum is not the place to discuss personnel issues or to state a negative opinion about an individual's managerial skills. Let's keep it cordial, we don't need to be bombarded with negativity here. Stacy -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, July 01, 2016 2:49 PM To: Terri Braud; 'histonet at lists.utsouthwestern.edu' Subject: [BULK] Re: [Histonet] Certification Importance: Low Your tech has an "above deserved" expectation.How would you even consider promoting somebody who is not qualified to even be HT certified to Lead HT/Coordinator?This is disrespectful for those who reversing that position have been unable to achieve it.It speaks volumes about your tech aspirations and your managerial skills.Ren? On Friday, July 1, 2016 1:50 PM, Terri Braud via Histonet wrote: Respectfully, a public forum is no place to discuss? personnel issues on such a personal level.? Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 ? 4. Certification (Adesupo, Adesuyi (Banjo)) ------------------------------ Message: 4 Date: Fri, 1 Jul 2016 09:45:31 -0500 From: "Adesupo, Adesuyi (Banjo)" To: "'histonet at lists.utsouthwestern.edu'" ??? Subject: [Histonet] Certification Hi, ? ? I hope you guys are doing great. Please I have a question and would appreciate your contributions. I have a tech that could not pass the certification test, when the ASCP were still accepting high school diploma for HT. The tech is no longer eligible to take the test again, because he did not have the minimum requirement (i.e. Associate Degree) to register for the test. He wants me to promote him to Histology Lead Tech/Histology Coordinator. What do you guys think about this? Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 Cell: 405-973-6363 ===================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 152, Issue 1 **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmmathis at wakehealth.edu Fri Jul 1 14:16:32 2016 From: cmmathis at wakehealth.edu (Cathy M. Mathis/Comparative Medicine) Date: Fri, 1 Jul 2016 19:16:32 +0000 Subject: [Histonet] CD34 for primate tissue Message-ID: <4816F916B11AEC4E92A0D01372F573E2010FFC8BA6@EXCHDB8.medctr.ad.wfubmc.edu> This is a great website for NHP info. Thank you all for all your wonderful help!! Hope everyone has a great 4th of July weekend!!!! Cathy -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Friday, July 01, 2016 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 152, Issue 1 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: How to get your stains more vibrant when cutting at 2?m? (Angela Lamberth) 2. Re: CD34 for primate tissue (Croix, Denise) 3. Just some before the long weekend Friday Fun-lab related blog (Lester Raff MD) 4. Certification (Adesupo, Adesuyi (Banjo)) ---------------------------------------------------------------------- Message: 1 Date: Thu, 30 Jun 2016 10:41:38 -0700 From: Angela Lamberth Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] How to get your stains more vibrant when cutting at 2?m? Message-ID: Content-Type: text/plain; charset=UTF-8 Thank you all very much for your suggestions. I'm going to play around with a progressive H&E when I return from vacation next month. I do have safranin on hand but will need to order some phloxine to experiment with. I will probably need to order some additional supplies to make the hematoxylin. I'm not sure yet if I'll start with Mayer's or Gill's or maybe even Erlich's. I should note that cutting at 2 isn't required, but it is desired once they saw that I can. And since I can, I aim to please! :-) In addition, I'm looking forward to trying the oven dry method before coverslipping. A rapid dehydration isn't really possible since I'm working with a xylene substitute (Pro-Par) and have been battling eosin carryover but that is a whole different animal for another thread. Thanks again! I'll report back in a month or 2. On Wed, Jun 29, 2016 at 10:27 AM, Rene J Buesa wrote: > > Angela: > "Pale" results are the trade-off for great quality very thin "2 ?m" > sections but you can always improve intensity somewhat . > 1- your "regressive" stain, if it is "modern Harris" has the inherent > problem of lacking mercury chloride and it is little you can do about. > Perhaps if you use "progressive Mayer" you will get better results. > You will not have to differentiate (with the intrinsic "danger" of > leaving the section too pale) and if used fresh Mayer's can be a good approach. > 2- as to the counterstain perhaps you should add safranine to the > eosin (20% safranine + 80% eosin) and will get a darker red. > 3- try to dehydrate as quickly as possible or even better, wash the > sections in distilled water and place them in an oven at 60?C for 10 > minutes and coverslip as usual. You will eliminate any "color wash" > due to the alcohols. > If you've not enough "trust" on dry/oven dehydration, try with some > sections as a test. You will like the method. > Ren? > > > On Tuesday, June 28, 2016 5:53 PM, Angela Lamberth via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > When I cut at 2?m my H&Es and special stains look pale. How can I get > my stains to pop or am I stuck with pale looking stains when > sectioning that thin? > > I run manual specials and a manual regressive H&E. For H&E I've tried > increasing my time in hematoxylin (beyond the manufacturer > recommendation), diluting my acid alcohol differentiation, and > increased time in eosin but the slides still lack the vibrancy that many of the postdocs desire. > > I use Shandon instant hematoxylin and alcoholic eosin by Thermo. > Everything else I prepare in house from scratch. Any recommendations? > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- Angela Lamberth Histology Technician II Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 ------------------------------ Message: 2 Date: Thu, 30 Jun 2016 16:57:53 -0500 From: "Croix, Denise" To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] CD34 for primate tissue Message-ID: Content-Type: text/plain; charset=UTF-8 Hi Cathy, When I was doing work with NHP tissue (both flow cytometry and IHC) there were several sources that I utilized for info on antibodies that were cross-reactivity with various NHP species. The NIH hosts a NHP reagent resource website (nhpreagents.org) that shows which commercially available antibodies cross react with non-human primate tissues. They note that there are discrepancies reported about cross-reactivity with the QBEND10 antibody for rhesus and possibly cynos. You will need to still check with the vendors to confirm if the antibody has been tested in FFPE specimens as that is not indicated on the site but it should give you a starting point to work from. I've had personal experience with cyno & rhesus T cells where individual monkeys would not react with certain anti-human antibodies. For example, I had a couple of research animals that were negative for a CD3 antibody but yet they stained with CD4 and CD8 antibodies. Good luck! Denise -- [image: Powered by Sigstr] *Denise A Croix, Ph.D.* Pathology Solutions Specialist - PD-L1 SP142 Assay Roche Diagnostics Corporation Tissue Diagnostics Division 972-207-7305 denise.croix at roche.com www.ventana.com [image: Powered by Sigstr] [image: Powered by Sigstr] ------------------------------ Message: 3 Date: Fri, 1 Jul 2016 13:44:10 +0000 From: Lester Raff MD To: "Histonet at lists.utsouthwestern.edu" Subject: [Histonet] Just some before the long weekend Friday Fun-lab related blog Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10E5E6FC at COLOEXCH01.uropartners.local> Content-Type: text/plain; charset="us-ascii" Pathologists couldn't do it without great histology teams, so thanks and have a Happy 4th. http://www.chicagonow.com/downsize-maybe/2016/06/reading-a-prostate-biopsy-inside-the-mind-of-a-pathologist/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ------------------------------ Message: 4 Date: Fri, 1 Jul 2016 09:45:31 -0500 From: "Adesupo, Adesuyi (Banjo)" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Certification Message-ID: <04EE4F75BB5FB246ADB68D69B7460443AF7A2CEA42 at MAIL.nrhnt.nrh-ok.com> Content-Type: text/plain; charset="us-ascii" Hi, I hope you guys are doing great. Please I have a question and would appreciate your contributions. I have a tech that could not pass the certification test, when the ASCP were still accepting high school diploma for HT. The tech is no longer eligible to take the test again, because he did not have the minimum requirement (i.e. Associate Degree) to register for the test. He wants me to promote him to Histology Lead Tech/Histology Coordinator. What do you guys think about this? Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 Cell: 405-973-6363 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 152, Issue 1 **************************************** From s.murdoch at dermpathnw.com Fri Jul 1 14:41:36 2016 From: s.murdoch at dermpathnw.com (Stacey Murdoch) Date: Fri, 1 Jul 2016 12:41:36 -0700 Subject: [Histonet] Certification In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F807E337@HRHEX02-HOS.holyredeemer.local> <121392237.2016074.1467398918399.JavaMail.yahoo@mail.yahoo.com> Message-ID: <5776c7e4.392ac80a.d6026.ffffc8ea@mx.google.com> If you have a great employee who has shown great potential to be a leader, but lacks the credentials to qualify for a position, perhaps you could assist your employee in obtaining the necessary education. Tuition assistance, flexibility in scheduling, etc. If the employee does not show the initiative to put the work in to qualify, but simply expects it to be handed to him, then perhaps he isn't a good fit. If however, you have a loyal, hardworking employee willing to make the effort, then it may behoove you to work with him. You may both benefit in the long run. Stacey -----Original Message----- From: Stacy McLaughlin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, July 01, 2016 12:10 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] [BULK] Re: Certification I will respectfully comment as well. A public forum is not the place to discuss personnel issues or to state a negative opinion about an individual's managerial skills. Let's keep it cordial, we don't need to be bombarded with negativity here. Stacy -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, July 01, 2016 2:49 PM To: Terri Braud; 'histonet at lists.utsouthwestern.edu' Subject: [BULK] Re: [Histonet] Certification Importance: Low Your tech has an "above deserved" expectation.How would you even consider promoting somebody who is not qualified to even be HT certified to Lead HT/Coordinator?This is disrespectful for those who reversing that position have been unable to achieve it.It speaks volumes about your tech aspirations and your managerial skills.Ren? On Friday, July 1, 2016 1:50 PM, Terri Braud via Histonet wrote: Respectfully, a public forum is no place to discuss personnel issues on such a personal level. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 4. Certification (Adesupo, Adesuyi (Banjo)) ------------------------------ Message: 4 Date: Fri, 1 Jul 2016 09:45:31 -0500 From: "Adesupo, Adesuyi (Banjo)" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Certification Hi, I hope you guys are doing great. Please I have a question and would appreciate your contributions. I have a tech that could not pass the certification test, when the ASCP were still accepting high school diploma for HT. The tech is no longer eligible to take the test again, because he did not have the minimum requirement (i.e. Associate Degree) to register for the test. He wants me to promote him to Histology Lead Tech/Histology Coordinator. What do you guys think about this? Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 Cell: 405-973-6363 ===================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 152, Issue 1 **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ZDurkin at goodwin.edu Tue Jul 5 09:14:39 2016 From: ZDurkin at goodwin.edu (Zoe Durkin) Date: Tue, 5 Jul 2016 14:14:39 +0000 Subject: [Histonet] Part time Histotechnician Position Message-ID: <1467728075269.69349@goodwin.edu> We have a part time histotechnician position available in Richmond, VA. Interested parties please email CV or resume to zdurkin at labpulsemed.com Thank you, Zoe Ann Durkin, M.Ed., HT(ASCP) [https://webmail.goodwin.edu/owa/service.svc/s/GetFileAttachment?id=AAMkAGRhOWYxNzBmLWNjZjctNDVmYy05ZDk5LTdlZDNjMzE5NTJjYQBGAAAAAABUZnvWtsXpRJyVgt2mVB3wBwAJCa8KV29lRbm06b6LzgYLAAAAOJ6oAABFQ0bZLhXVSqffXA2yJJT1AAB2tpBiAAABEgAQAKTuwGqRD%2BVBoOJrtwqqFr8%3D&isImagePreview=True&X-OWA-CANARY=INMYuOOLeE6UEE152Tw3x-N_dTKRG9MI8Y7vbv1wTBvsam6-a6yPMENHZlKqx7pPlCgVB6ey-cE.] From annamhuntley at gmail.com Tue Jul 5 12:36:03 2016 From: annamhuntley at gmail.com (Anna Huntley Coffey) Date: Tue, 5 Jul 2016 13:36:03 -0400 Subject: [Histonet] Samples not staining with Hematoxylin or primary Message-ID: Hello Histonetters, I'm having an issue with some recent samples and was wondering if anyone else has experienced the same thing...I stained some samples with a previously optimized protocol for anti-human Granzyme B on the Ventana Benchmark and about 1/3 of my samples did not stain at all with either GranzB or Hematoxylin. My controls and samples that did stain positive for GranzB look fine and the machine has been recently serviced and working well with other runs. I'd really appreciate your input if you have any advice or suggestions on what could have gone wrong. Thanks, Anna From tilycyn at auburn.edu Tue Jul 5 12:46:14 2016 From: tilycyn at auburn.edu (Cynthia Hutchinson) Date: Tue, 5 Jul 2016 17:46:14 +0000 Subject: [Histonet] full time job opportunity Message-ID: <1067ef1edf1b4d299466b10f00d08708@ex5.auburn.edu> Auburn University has a full time histotech position in the veterinary diagnostic lab. Please see link for details: https://www.auemployment.com/applicants/jsp/shared/frameset/Frameset.jsp?time=1467740688882 Cynthia Tily Hutchinson Rsch Asst IV Pathobiology 166 Greene Hall Coll of Vet Med Auburn Univ 36849 (334)844 7020 From akbitting at geisinger.edu Tue Jul 5 15:06:47 2016 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Tue, 5 Jul 2016 20:06:47 +0000 Subject: [Histonet] curiosity may kill me Message-ID: I'm curious as to why we heat the Auramine Rhodamine when we are staining tissue sections but our Micro lab stains at room temp. It is because "it's always been done that way"? Thanks group, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From relia1 at earthlink.net Wed Jul 6 08:35:32 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 6 Jul 2016 09:35:32 -0400 Subject: [Histonet] RELIA Hot Histology Job Alert 7-6-2016 Exciting IHC position in CA Message-ID: <00a701d1d78b$45491b20$cfdb5160$@earthlink.net> Hi Histopeeps! It?s hump day and a short week what more could you ask for? How about an exciting new job opportunity? RELIA Solutions has been EXCLUSIVELY engaged by a growing private lab in CA that is in need of an IHC tech. This is a full time permanent position and my client offers an excellent compensation package including a competitive salary, great benefits relocation assistance and an amazing team to work with. They are in need of an ASCP HT/HTL certified histotech with strong cutting skills to work in their fully automated IHC lab. At least 2 years of histology experience is required. My client is ready to interview and hire for this opportunity. If this is the right job for you RELIA can make it happen. For more information and the EXACT location please contact Pam Barker at relia1 at earthlink.net or toll free at 866-607-3542 or cell/text 407-353-5070. RELIA Solutions is the nation's ONLY recruiting firm specializing in the nationwide permanent placement of histology professionals. To sign up for our free histology careers bulletin please send an email to relia1 at earthlink.net and include subscribe in the subject line. Keywords: Histology, histologist, histotechnician, histotechnologist, immunohistochemistry, IHC, CA Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From rjbuesa at yahoo.com Wed Jul 6 09:15:38 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 6 Jul 2016 14:15:38 +0000 (UTC) Subject: [Histonet] curiosity may kill me In-Reply-To: References: Message-ID: <658963699.3851527.1467814538135.JavaMail.yahoo@mail.yahoo.com> I always used Auramine at room temperature to identify TB bacilli in tissue sections with fluorescence filter, never used heat and the results were as expected.?Bancroft is the one describing the procedure using heat for?auramine/rhodamine procedure but auramine alone at room temperature is enough.Ren? On Tuesday, July 5, 2016 4:24 PM, "Bitting, Angela K. via Histonet" wrote: I'm curious as to why we heat the Auramine Rhodamine when we are staining tissue sections but our Micro lab stains at room temp.? It is because "it's always been done that way"? Thanks group, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annamhuntley at gmail.com Wed Jul 6 11:53:19 2016 From: annamhuntley at gmail.com (Anna Huntley Coffey) Date: Wed, 6 Jul 2016 12:53:19 -0400 Subject: [Histonet] IHC antibody/assay recommendations for IL-4, IL-13, IL-4R, and IL-13Ra1 Message-ID: Just wondering if anyone has had good luck with any of these antibodies (IL-4, IL-13, IL-4R, and IL-13Ra1) and would be willing to share vendor info and/or assay conditions they've tested? From craigak12 at gmail.com Wed Jul 6 12:23:18 2016 From: craigak12 at gmail.com (J B) Date: Wed, 06 Jul 2016 17:23:18 +0000 Subject: [Histonet] Document control system: Message-ID: Does anyone have experience with Media Labs document control system? I am looking for good software for documentation control. Any feedback is appreciated. Sincerely, JB From tejohnson at genoptix.com Wed Jul 6 12:55:30 2016 From: tejohnson at genoptix.com (Teri Johnson) Date: Wed, 6 Jul 2016 17:55:30 +0000 Subject: [Histonet] Samples not staining with Hematoxylin or primary Message-ID: <6e205ab6e3544c63abb55c8fec2da201@PHUSCB-SP37MB01.genoptix.org> Anna wrote: I'm curious as to why we heat the Auramine Rhodamine when we are staining tissue sections but our Micro lab stains at room temp. It is because "it's always been done that way"? Thanks group, Angie Hi Anna, If your dispensers are working properly, I would bet it's your slides. There are known issues with overly hydrophobic slides causing issues on the Ventana platform. They recommend you use Plus (+) slides manufactured from Erie Scientific. There may be others I am not aware of, but we use these slides for all IHC sectioned here and have had no issues on our Ventanas. Good luck! Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From LRaff at uropartners.com Wed Jul 6 13:12:32 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 6 Jul 2016 18:12:32 +0000 Subject: [Histonet] Media Lab Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10E78B6A@COLOEXCH01.uropartners.local> We have been using MediaLab for several years, beginning with their online courses and progressing to Document Control and InspectionProof. We are awaiting our first CAP inspection in which I expect the inspectors to be able to get virtually all the information they need from our MediaLab site. Also, customer support is great! It took a bit of time for the lab staff to accept the concept of online manuals and SOP's but in the long run it is a big win. -------------------------- Latest blog post: http://www.chicagonow.com/downsize-maybe/2016/07/after-watching-independence-day-resurgence-i-wonder-could-will-smith-get-me-through-an-apocalypse/ -------------------------- Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: J B via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, July 06, 2016 12:23 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Document control system: Does anyone have experience with Media Labs document control system? I am looking for good software for documentation control. Any feedback is appreciated. Sincerely, JB _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From craigak12 at gmail.com Wed Jul 6 13:47:56 2016 From: craigak12 at gmail.com (J B) Date: Wed, 06 Jul 2016 18:47:56 +0000 Subject: [Histonet] Media Lab In-Reply-To: <6347C6D2B080534F9B5C2B08436DCFAF10E78B6A@COLOEXCH01.uropartners.local> References: <6347C6D2B080534F9B5C2B08436DCFAF10E78B6A@COLOEXCH01.uropartners.local> Message-ID: Thank you for the feedback. Was it hard or time consuming to upload all procedures? I will only have a few weeks before my CAP window starts. Thank you, JB On Wed, Jul 6, 2016, 11:26 AM Lester Raff MD via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > > We have been using MediaLab for several years, beginning with their online > courses and progressing to Document Control and InspectionProof. We are > awaiting our first CAP inspection in which I expect the inspectors to be > able to get virtually all the information they need from our MediaLab site. > Also, customer support is great! > > > > It took a bit of time for the lab staff to accept the concept of online > manuals and SOP's but in the long run it is a big win. > > > > -------------------------- > > > > Latest blog post: > http://www.chicagonow.com/downsize-maybe/2016/07/after-watching-independence-day-resurgence-i-wonder-could-will-smith-get-me-through-an-apocalypse/ > > > > -------------------------- > > Lester J. Raff, MD MBA > > UroPartners > > Medical Director Of Laboratory > > 2225 Enterprise Dr. Suite 2511 > > Westchester, Il 60154 > > Tel: 708-486-0076 > > Fax: 708-492-0203 > > > > -----Original Message----- > From: J B via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, July 06, 2016 12:23 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Document control system: > > > > Does anyone have experience with Media Labs document control system? I am > looking for good software for documentation control. Any feedback is > appreciated. > > > > Sincerely, > > > > JB > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sbaldwin at mhhcc.org Wed Jul 6 15:12:30 2016 From: sbaldwin at mhhcc.org (Baldwin, Kathy) Date: Wed, 6 Jul 2016 20:12:30 +0000 Subject: [Histonet] MITF Message-ID: <1163fb0a386e4a22afebbd3d8d841c67@exch02.mhhcc.org> Hi Histonetters Was wondering if anyone has had any experience with MITF what controls would you use I have a fairly new pathologist that wants us to use Nevus' instead on melanoma and half the cases are staining and the other half are not what is your experience? Any help would be greatly appreciated! Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 Cell 812-887-3357 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From annamhuntley at gmail.com Wed Jul 6 15:14:26 2016 From: annamhuntley at gmail.com (Anna Huntley Coffey) Date: Wed, 6 Jul 2016 16:14:26 -0400 Subject: [Histonet] Samples not staining with Hematoxylin or primary Message-ID: Thanks so much to everyone who responded! A couple people have suggested that this issue may be due to a disruption to the charge on the slide (due to high temp baking, water bath additives, etc) which causes a hydrophobic barrier to form and prevents reagent from reaching the tissue. This sounds very convincing to me, but we have found that repeated antigen retrieval using a pressure cooker (our standard retrieval for this particular primary) has allowed 2 of the 3 slides to stain with both DAB and Hematoxylin. Would repeated retrieval be able to reverse the slide charge back to normal? I'm having an issue with some recent samples and was wondering if anyone else has experienced the same thing...I stained some samples with a previously optimized protocol for anti-human Granzyme B on the Ventana Benchmark and about 1/3 of my samples did not stain at all with either GranzB or Hematoxylin. My controls and samples that did stain positive for GranzB look fine and the machine has been recently serviced and working well with other runs. I'd really appreciate your input if you have any advice or suggestions on what could have gone wrong. Thanks, Anna From cjohnson at nmda.nmsu.edu Wed Jul 6 16:25:16 2016 From: cjohnson at nmda.nmsu.edu (Johnson, Carole) Date: Wed, 6 Jul 2016 21:25:16 +0000 Subject: [Histonet] CWD testing Message-ID: I would like to hear from people who are doing CWD testing on biopsies (tonsil or rectal). Are you placing a disclaimer on results, and if so do you include the number of follicles present in the specimen? You can answer privately at: cjohnson at nmda.nmsu.edu Thanks for your input Carole L. Johnson, HT(ASCP)cm, QIHC New Mexico Department of Agriculture Veterinary Diagnositc Services 1101 Camino de Salud, NE Albuquerque, NM 87101 505.383.9299 Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From edmartin26 at gmail.com Wed Jul 6 23:45:01 2016 From: edmartin26 at gmail.com (Eddie Martin) Date: Wed, 6 Jul 2016 23:45:01 -0500 Subject: [Histonet] Why is Auromine heated in Histology, but not in Microbiology Message-ID: <077D5DFB-890A-4ACD-89F9-8EC66EB3B8B1@gmail.com> Hi Angie, The simplest answer, would be to save time. Whether heated, or microwaved at 80 power for 45 seconds, would be quicker than flooding the slide and letting stand for extended periods of time. From edmartin26 at gmail.com Thu Jul 7 00:46:25 2016 From: edmartin26 at gmail.com (Eddie Martin) Date: Thu, 7 Jul 2016 00:46:25 -0500 Subject: [Histonet] MITF Message-ID: <24F35FB0-A7E9-4261-BDE0-27B4F1AD85B1@gmail.com> Hi Kathy, The Journal of Investigative Dermatology had an article in their December 2011 Volume 131 issue 12 on Human Cutaneous Melanomas lacking MITF and melanocyte differentiation. According to the article, Melanomas could be MITF and Melan-A negative, if the Melanomas were TAM tyrosine kinase receptor positive. In this particular study, 38% of the total Cutaneous Melanomas were Axl+ (member of TAM), and none of the Axl+ were MITF and Melan-A positive. That being said, it may explain why some of your cutaneous melanomas are not MITF positive. Known internal control tissue for MITF includes epithelioid tissue, mast cells, reactive histiocytes and osteoclasts stain intensely for MITF. A nevus would also be an ideal control tissue, as would any other perivascular epithelioid tumor. Best, Eddie Martin, HTL, HT(ASCP), QIHC From Diana.Martinez-Longoria at ecrmc.org Thu Jul 7 07:49:29 2016 From: Diana.Martinez-Longoria at ecrmc.org (Diana Martinez-Longoria) Date: Thu, 7 Jul 2016 12:49:29 +0000 Subject: [Histonet] Special Stain Message-ID: <12B71261212BE94BB9FB7735484B9FA178C8B208@EXMBX01.ecrmc.ci.el-centro.ca.us> Good morning everyone, I was wondering if anyone can help us in regards to special stain contamination. We will like to know if contamination can happen as a result of reagents touching the writing of a marking pen on a slide? For example we are manually running a slide that has the typical marking pen writing on the slide for identification and we are doing a silver stain, will it contaminate and not give an accurate result? I know this might be a weird question, but any information, resources, documentation you can direct me to would be very much appreciated. Please email me privately at dmlongoria at ecrmc.org. Thank you! ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? From gmartin at marshallmedical.org Thu Jul 7 12:22:23 2016 From: gmartin at marshallmedical.org (Martin, Gary) Date: Thu, 7 Jul 2016 10:22:23 -0700 Subject: [Histonet] Histonet Digest, Vol 152, Issue 5 In-Reply-To: References: Message-ID: <6ED9D4252F278841A0593D3D788AF24C366E5D38@mailsvr.MARSHMED.local> We are also interested in a reasonablly price and simple document control system. Thanks Gary -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, July 07, 2016 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 152, Issue 5 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Document control system: (J B) 2. Re: Samples not staining with Hematoxylin or primary (Teri Johnson) 3. Re: Media Lab (Lester Raff MD) 4. Re: Media Lab (J B) 5. MITF (Baldwin, Kathy) 6. Samples not staining with Hematoxylin or primary (Anna Huntley Coffey) 7. CWD testing (Johnson, Carole) 8. Why is Auromine heated in Histology, but not in Microbiology (Eddie Martin) 9. Re: MITF (Eddie Martin) 10. Special Stain (Diana Martinez-Longoria) ---------------------------------------------------------------------- Message: 1 Date: Wed, 06 Jul 2016 17:23:18 +0000 From: J B To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Document control system: Message-ID: Content-Type: text/plain; charset=UTF-8 Does anyone have experience with Media Labs document control system? I am looking for good software for documentation control. Any feedback is appreciated. Sincerely, JB ------------------------------ Message: 2 Date: Wed, 6 Jul 2016 17:55:30 +0000 From: Teri Johnson To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Samples not staining with Hematoxylin or primary Message-ID: <6e205ab6e3544c63abb55c8fec2da201 at PHUSCB-SP37MB01.genoptix.org> Content-Type: text/plain; charset=WINDOWS-1252 Anna wrote: I'm curious as to why we heat the Auramine Rhodamine when we are staining tissue sections but our Micro lab stains at room temp. It is because "it's always been done that way"? Thanks group, Angie Hi Anna, If your dispensers are working properly, I would bet it's your slides. There are known issues with overly hydrophobic slides causing issues on the Ventana platform. They recommend you use Plus (+) slides manufactured from Erie Scientific. There may be others I am not aware of, but we use these slides for all IHC sectioned here and have had no issues on our Ventanas. Good luck! Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. ------------------------------ Message: 3 Date: Wed, 6 Jul 2016 18:12:32 +0000 From: Lester Raff MD To: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Media Lab Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10E78B6A at COLOEXCH01.uropartners.local> Content-Type: text/plain; charset="us-ascii" We have been using MediaLab for several years, beginning with their online courses and progressing to Document Control and InspectionProof. We are awaiting our first CAP inspection in which I expect the inspectors to be able to get virtually all the information they need from our MediaLab site. Also, customer support is great! It took a bit of time for the lab staff to accept the concept of online manuals and SOP's but in the long run it is a big win. -------------------------- Latest blog post: http://www.chicagonow.com/downsize-maybe/2016/07/after-watching-independence-day-resurgence-i-wonder-could-will-smith-get-me-through-an-apocalypse/ -------------------------- Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: J B via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, July 06, 2016 12:23 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Document control system: Does anyone have experience with Media Labs document control system? I am looking for good software for documentation control. Any feedback is appreciated. Sincerely, JB _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ------------------------------ Message: 4 Date: Wed, 06 Jul 2016 18:47:56 +0000 From: J B To: Lester Raff MD , "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Media Lab Message-ID: Content-Type: text/plain; charset=UTF-8 Thank you for the feedback. Was it hard or time consuming to upload all procedures? I will only have a few weeks before my CAP window starts. Thank you, JB On Wed, Jul 6, 2016, 11:26 AM Lester Raff MD via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > > We have been using MediaLab for several years, beginning with their online > courses and progressing to Document Control and InspectionProof. We are > awaiting our first CAP inspection in which I expect the inspectors to be > able to get virtually all the information they need from our MediaLab site. > Also, customer support is great! > > > > It took a bit of time for the lab staff to accept the concept of online > manuals and SOP's but in the long run it is a big win. > > > > -------------------------- > > > > Latest blog post: > http://www.chicagonow.com/downsize-maybe/2016/07/after-watching-independence-day-resurgence-i-wonder-could-will-smith-get-me-through-an-apocalypse/ > > > > -------------------------- > > Lester J. Raff, MD MBA > > UroPartners > > Medical Director Of Laboratory > > 2225 Enterprise Dr. Suite 2511 > > Westchester, Il 60154 > > Tel: 708-486-0076 > > Fax: 708-492-0203 > > > > -----Original Message----- > From: J B via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, July 06, 2016 12:23 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Document control system: > > > > Does anyone have experience with Media Labs document control system? I am > looking for good software for documentation control. Any feedback is > appreciated. > > > > Sincerely, > > > > JB > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 5 Date: Wed, 6 Jul 2016 20:12:30 +0000 From: "Baldwin, Kathy" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] MITF Message-ID: <1163fb0a386e4a22afebbd3d8d841c67 at exch02.mhhcc.org> Content-Type: text/plain; charset="us-ascii" Hi Histonetters Was wondering if anyone has had any experience with MITF what controls would you use I have a fairly new pathologist that wants us to use Nevus' instead on melanoma and half the cases are staining and the other half are not what is your experience? Any help would be greatly appreciated! Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 Cell 812-887-3357 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 6 Date: Wed, 6 Jul 2016 16:14:26 -0400 From: Anna Huntley Coffey To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Samples not staining with Hematoxylin or primary Message-ID: Content-Type: text/plain; charset=UTF-8 Thanks so much to everyone who responded! A couple people have suggested that this issue may be due to a disruption to the charge on the slide (due to high temp baking, water bath additives, etc) which causes a hydrophobic barrier to form and prevents reagent from reaching the tissue. This sounds very convincing to me, but we have found that repeated antigen retrieval using a pressure cooker (our standard retrieval for this particular primary) has allowed 2 of the 3 slides to stain with both DAB and Hematoxylin. Would repeated retrieval be able to reverse the slide charge back to normal? I'm having an issue with some recent samples and was wondering if anyone else has experienced the same thing...I stained some samples with a previously optimized protocol for anti-human Granzyme B on the Ventana Benchmark and about 1/3 of my samples did not stain at all with either GranzB or Hematoxylin. My controls and samples that did stain positive for GranzB look fine and the machine has been recently serviced and working well with other runs. I'd really appreciate your input if you have any advice or suggestions on what could have gone wrong. Thanks, Anna ------------------------------ Message: 7 Date: Wed, 6 Jul 2016 21:25:16 +0000 From: "Johnson, Carole" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] CWD testing Message-ID: Content-Type: text/plain; charset="us-ascii" I would like to hear from people who are doing CWD testing on biopsies (tonsil or rectal). Are you placing a disclaimer on results, and if so do you include the number of follicles present in the specimen? You can answer privately at: cjohnson at nmda.nmsu.edu Thanks for your input Carole L. Johnson, HT(ASCP)cm, QIHC New Mexico Department of Agriculture Veterinary Diagnositc Services 1101 Camino de Salud, NE Albuquerque, NM 87101 505.383.9299 Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. ------------------------------ Message: 8 Date: Wed, 6 Jul 2016 23:45:01 -0500 From: Eddie Martin To: "histonet (histonet at lists.utsouthwestern.edu)" Subject: [Histonet] Why is Auromine heated in Histology, but not in Microbiology Message-ID: <077D5DFB-890A-4ACD-89F9-8EC66EB3B8B1 at gmail.com> Content-Type: text/plain; charset=us-ascii Hi Angie, The simplest answer, would be to save time. Whether heated, or microwaved at 80 power for 45 seconds, would be quicker than flooding the slide and letting stand for extended periods of time. ------------------------------ Message: 9 Date: Thu, 7 Jul 2016 00:46:25 -0500 From: Eddie Martin To: "histonet (histonet at lists.utsouthwestern.edu)" Subject: Re: [Histonet] MITF Message-ID: <24F35FB0-A7E9-4261-BDE0-27B4F1AD85B1 at gmail.com> Content-Type: text/plain; charset=us-ascii Hi Kathy, The Journal of Investigative Dermatology had an article in their December 2011 Volume 131 issue 12 on Human Cutaneous Melanomas lacking MITF and melanocyte differentiation. According to the article, Melanomas could be MITF and Melan-A negative, if the Melanomas were TAM tyrosine kinase receptor positive. In this particular study, 38% of the total Cutaneous Melanomas were Axl+ (member of TAM), and none of the Axl+ were MITF and Melan-A positive. That being said, it may explain why some of your cutaneous melanomas are not MITF positive. Known internal control tissue for MITF includes epithelioid tissue, mast cells, reactive histiocytes and osteoclasts stain intensely for MITF. A nevus would also be an ideal control tissue, as would any other perivascular epithelioid tumor. Best, Eddie Martin, HTL, HT(ASCP), QIHC ------------------------------ Message: 10 Date: Thu, 7 Jul 2016 12:49:29 +0000 From: Diana Martinez-Longoria To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Special Stain Message-ID: <12B71261212BE94BB9FB7735484B9FA178C8B208 at EXMBX01.ecrmc.ci.el-centro.ca.us> Content-Type: text/plain; charset="us-ascii" Good morning everyone, I was wondering if anyone can help us in regards to special stain contamination. We will like to know if contamination can happen as a result of reagents touching the writing of a marking pen on a slide? For example we are manually running a slide that has the typical marking pen writing on the slide for identification and we are doing a silver stain, will it contaminate and not give an accurate result? I know this might be a weird question, but any information, resources, documentation you can direct me to would be very much appreciated. Please email me privately at dmlongoria at ecrmc.org. Thank you! ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 152, Issue 5 **************************************** From thigginsht at msn.com Thu Jul 7 13:46:37 2016 From: thigginsht at msn.com (T H) Date: Thu, 7 Jul 2016 18:46:37 +0000 Subject: [Histonet] Histonet Digest, Vol 152, Issue 5 In-Reply-To: References: Message-ID: I would place a piece of both the Nevus and the Melanoma in my control block, it should give you peace of mind that stain is working. The only problem I see with using a known MITF positive Melanoma control would be under staining cases that have weaker positivity. TH Message: 5 Date: Wed, 6 Jul 2016 20:12:30 +0000 From: "Baldwin, Kathy" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] MITF Message-ID: <1163fb0a386e4a22afebbd3d8d841c67 at exch02.mhhcc.org> Content-Type: text/plain; charset="us-ascii" Hi Histonetters Was wondering if anyone has had any experience with MITF what controls would you use I have a fairly new pathologist that wants us to use Nevus' instead on melanoma and half the cases are staining and the other half are not what is your experience? Any help would be greatly appreciated! Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 Cell 812-887-3357 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From sk at personifysearch.com Thu Jul 7 15:22:55 2016 From: sk at personifysearch.com (Sarah Kelly) Date: Thu, 7 Jul 2016 16:22:55 -0400 Subject: [Histonet] New Histology Career Opportunities - Cancer Diagnostics Market Leader Message-ID: <6227d1800e10e66928b6173fc61462fe@mail.gmail.com> Hello Histonet! Our exclusively retained client has opened several new opportunities across the U.S. (primarily in the Southeast and West Coast), and I wanted to reach out regarding a few specifics and see if anyone would be interested in learning more. Our client is a global leader in cancer diagnostics and we are looking for individuals with a strong histology background, IHC, and troubleshooting experience. The company is part of a $20 billion organization and growing. These positions are full-time, direct-hire roles with competitive salaries and full benefits. If you or anyone you know may be interested in learning more about these opportunities, please contact me directly at *sk at personifysearch.com *. Thank you! Sarah Sarah Kelly Personify, Talent Management Executive *sk at personifysearch.com * (800) 875-6188, ext.153 From af46 at buffalo.edu Fri Jul 8 08:53:45 2016 From: af46 at buffalo.edu (Featherstone, Annette) Date: Fri, 8 Jul 2016 13:53:45 +0000 Subject: [Histonet] Indirect Fluorescence on FFPE tissue Message-ID: Does anyone have a protocol for Indirect Fluorescence on FFPE tissue, on the Leica Bond? With or without amplification? Annette Featherstone From Pamela.S.Younes at uth.tmc.edu Fri Jul 8 12:06:38 2016 From: Pamela.S.Younes at uth.tmc.edu (Younes, Pamela S) Date: Fri, 8 Jul 2016 17:06:38 +0000 Subject: [Histonet] Frosted slides Message-ID: <8774B764-337A-4D1B-B20D-D9A2E80546F7@uth.tmc.edu> Hello, We are looking for completely frosted slides to use for cytopathology. Does anyone know a vendor? We have heard that some labs with purchase clear slides, and sand-blast them, but that sounds like a difficult process. Many thanks, Pam Younes Pamela Younes MHS, HTL(ASCP), CPC, PA(ASCP) Pathologists? Assistant, Assistant Professor University of Texas Health Science Center Houston, McGovern Campus Department of Pathology and Laboratory Medicine, 6431 Fannin, MSB 2233A, Houston, TX 77030 pamela.s.younes at uth.tmc.edu From paula at excaliburpathology.com Fri Jul 8 12:42:39 2016 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Fri, 8 Jul 2016 17:42:39 +0000 (UTC) Subject: [Histonet] Frosted slides In-Reply-To: <8774B764-337A-4D1B-B20D-D9A2E80546F7@uth.tmc.edu> References: <8774B764-337A-4D1B-B20D-D9A2E80546F7@uth.tmc.edu> Message-ID: <2106040174.4008035.1467999759115.JavaMail.yahoo@mail.yahoo.com> Hi, they are called Dakin slides.? A quick search found:?http://www.emsdiasum.com/microscopy/products/preparation/slides.aspx ?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: "Younes, Pamela S via Histonet" To: "histonet at lists.utsouthwestern.edu" Sent: Friday, July 8, 2016 12:06 PM Subject: [Histonet] Frosted slides Hello, We are looking for completely frosted slides to use for cytopathology.? Does anyone know a vendor?? We have heard that some labs with purchase clear slides, and sand-blast them, but that sounds like a difficult process. Many thanks, Pam Younes Pamela Younes MHS, HTL(ASCP), CPC, PA(ASCP) Pathologists? Assistant, Assistant Professor University of Texas Health Science Center Houston, McGovern Campus Department of Pathology and Laboratory Medicine, 6431 Fannin, MSB 2233A, Houston, TX 77030 pamela.s.younes at uth.tmc.edu _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann at aol.com Sat Jul 9 12:15:43 2016 From: thisisann at aol.com (Ann Specian) Date: Sat, 9 Jul 2016 13:15:43 -0400 Subject: [Histonet] microtome validation Message-ID: <155d0a85e33-212e-1dadc@webprd-m79.mail.aol.com> Has anyone had to validate a microtome (manual/automatic). If so, how did you do it? Ann From robert.jacox at thermofisher.com Sat Jul 9 12:53:24 2016 From: robert.jacox at thermofisher.com (Jacox, Robert A.) Date: Sat, 9 Jul 2016 17:53:24 +0000 Subject: [Histonet] microtome validation In-Reply-To: <155d0a85e33-212e-1dadc@webprd-m79.mail.aol.com> References: <155d0a85e33-212e-1dadc@webprd-m79.mail.aol.com> Message-ID: Ann, I would recommend validating manual microtomes more often then automatic. Any traditional gear driven microtome does change overtime as gears wear. This eventually will lead to the micron indicator on the microtome not reading what you are truly cutting. Automated, microtomes driven by stepper motors tend to remain accurate longer but you may want to document the accuracy every couple of years. To check calibration most service engineers will mount a micrometer to the microtome and measure the feed of the microtome as the hand wheel turns. The procedure generally takes 5 minutes. Check with your microtome manufacture to see if they can assist you. Robert Jacox Thermo Fisher Scientific Commercial Marketing Manager Sent from my iPad > On Jul 9, 2016, at 1:39 PM, Ann Specian via Histonet wrote: > > Has anyone had to validate a microtome (manual/automatic). If so, how did you do it? > Ann > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lmdee1 at yahoo.com Sat Jul 9 18:53:23 2016 From: lmdee1 at yahoo.com (Linda) Date: Sat, 9 Jul 2016 23:53:23 +0000 (UTC) Subject: [Histonet] Start up Vet/Animal histology laboratory References: <1990575214.1453277.1468108403616.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1990575214.1453277.1468108403616.JavaMail.yahoo@mail.yahoo.com> Hello Histoland, I am inquiring if anyone has started their own lab to?do histology services for veterinarians?or animal research?tissues? Or anyone who has started a private lab? What are your experiences?? What didn't you expect?? Thank you in advance. Regards, Linda Dee, BGS,HT(ASCP)?Lmdee1 at yahoo.com From twebster at CRH.org Sun Jul 10 15:22:34 2016 From: twebster at CRH.org (Webster, Thomas S.) Date: Sun, 10 Jul 2016 20:22:34 +0000 Subject: [Histonet] Histonet Digest, Vol 152, Issue 7 In-Reply-To: References: Message-ID: <7207186ED68FB542803CAF1CE6E82FF8763CC86B@exmb1.crh.org> We buy Dakin slides off of Cardinal health. http://www.cardinalhealth.com/en/product-solutions/medical/laboratory-products/anatomic-pathology/slides/dakin-slides.html ________________________________________ From: histonet-request at lists.utsouthwestern.edu [histonet-request at lists.utsouthwestern.edu] Sent: Saturday, July 09, 2016 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 152, Issue 7 Do not open unsolicited message attachments or click unknown links in email. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Frosted slides (Younes, Pamela S) 2. Re: Frosted slides (Paula Keene Pierce) ---------------------------------------------------------------------- Message: 1 Date: Fri, 8 Jul 2016 17:06:38 +0000 From: "Younes, Pamela S" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Frosted slides Message-ID: <8774B764-337A-4D1B-B20D-D9A2E80546F7 at uth.tmc.edu> Content-Type: text/plain; charset="utf-8" Hello, We are looking for completely frosted slides to use for cytopathology. Does anyone know a vendor? We have heard that some labs with purchase clear slides, and sand-blast them, but that sounds like a difficult process. Many thanks, Pam Younes Pamela Younes MHS, HTL(ASCP), CPC, PA(ASCP) Pathologists? Assistant, Assistant Professor University of Texas Health Science Center Houston, McGovern Campus Department of Pathology and Laboratory Medicine, 6431 Fannin, MSB 2233A, Houston, TX 77030 pamela.s.younes at uth.tmc.edu ------------------------------ Message: 2 Date: Fri, 8 Jul 2016 17:42:39 +0000 (UTC) From: Paula Keene Pierce To: "Younes, Pamela S" , Histonet Subject: Re: [Histonet] Frosted slides Message-ID: <2106040174.4008035.1467999759115.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Hi, they are called Dakin slides.? A quick search found:?http://www.emsdiasum.com/microscopy/products/preparation/slides.aspx ?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: "Younes, Pamela S via Histonet" To: "histonet at lists.utsouthwestern.edu" Sent: Friday, July 8, 2016 12:06 PM Subject: [Histonet] Frosted slides Hello, We are looking for completely frosted slides to use for cytopathology.? Does anyone know a vendor?? We have heard that some labs with purchase clear slides, and sand-blast them, but that sounds like a difficult process. Many thanks, Pam Younes Pamela Younes MHS, HTL(ASCP), CPC, PA(ASCP) Pathologists? Assistant, Assistant Professor University of Texas Health Science Center Houston, McGovern Campus Department of Pathology and Laboratory Medicine, 6431 Fannin, MSB 2233A, Houston, TX 77030 pamela.s.younes at uth.tmc.edu _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 152, Issue 7 **************************************** CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From badams at acadianagastro.com Sun Jul 10 15:58:09 2016 From: badams at acadianagastro.com (Brent Adams) Date: Sun, 10 Jul 2016 20:58:09 +0000 Subject: [Histonet] Start up lab In-Reply-To: References: Message-ID: <985vi52q2p312iu8w71fbpy5.1468184286300@email.android.com> I started a lab with the help of Miraca Life Science. Policies and procedures is probably the toughest. Finding a vender to take xylene waste an getting haz waste credentials. CLIA certifies & monitors the lab. I used SharpsInc.com for my biohazard as my volume was to low to use stericycle and not cost effective. doing everything yourself is not easy but can be done but your output is low due to the amount of taskes to be completed. hope that helps. Brent @ Acadiana Gastroenterology Assc Lafayette, LA Sent from my Verizon, Samsung Galaxy smartphone -------- Original message -------- From: histonet-request at lists.utsouthwestern.edu Date: 7/10/16 12:16 PM (GMT-06:00) To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 152, Issue 8 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. microtome validation (Ann Specian) 2. Re: microtome validation (Jacox, Robert A.) 3. Start up Vet/Animal histology laboratory (Linda) ---------------------------------------------------------------------- Message: 1 Date: Sat, 9 Jul 2016 13:15:43 -0400 From: Ann Specian To: histonet at lists.utsouthwestern.edu Subject: [Histonet] microtome validation Message-ID: <155d0a85e33-212e-1dadc at webprd-m79.mail.aol.com> Content-Type: text/plain; charset=utf-8 Has anyone had to validate a microtome (manual/automatic). If so, how did you do it? Ann ------------------------------ Message: 2 Date: Sat, 9 Jul 2016 17:53:24 +0000 From: "Jacox, Robert A." To: Ann Specian Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] microtome validation Message-ID: Content-Type: text/plain; charset="us-ascii" Ann, I would recommend validating manual microtomes more often then automatic. Any traditional gear driven microtome does change overtime as gears wear. This eventually will lead to the micron indicator on the microtome not reading what you are truly cutting. Automated, microtomes driven by stepper motors tend to remain accurate longer but you may want to document the accuracy every couple of years. To check calibration most service engineers will mount a micrometer to the microtome and measure the feed of the microtome as the hand wheel turns. The procedure generally takes 5 minutes. Check with your microtome manufacture to see if they can assist you. Robert Jacox Thermo Fisher Scientific Commercial Marketing Manager Sent from my iPad > On Jul 9, 2016, at 1:39 PM, Ann Specian via Histonet wrote: > > Has anyone had to validate a microtome (manual/automatic). If so, how did you do it? > Ann > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sat, 9 Jul 2016 23:53:23 +0000 (UTC) From: Linda To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Start up Vet/Animal histology laboratory Message-ID: <1990575214.1453277.1468108403616.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Hello Histoland, I am inquiring if anyone has started their own lab to?do histology services for veterinarians?or animal research?tissues? Or anyone who has started a private lab? What are your experiences?? What didn't you expect?? Thank you in advance. Regards, Linda Dee, BGS,HT(ASCP)?Lmdee1 at yahoo.com ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 152, Issue 8 **************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. From vapatpxs at yahoo.com Sun Jul 10 16:44:26 2016 From: vapatpxs at yahoo.com (Va Paula Sicurello) Date: Sun, 10 Jul 2016 21:44:26 +0000 (UTC) Subject: [Histonet] Start up Vet/Animal histology laboratory In-Reply-To: <1990575214.1453277.1468108403616.JavaMail.yahoo@mail.yahoo.com> References: <1990575214.1453277.1468108403616.JavaMail.yahoo.ref@mail.yahoo.com> <1990575214.1453277.1468108403616.JavaMail.yahoo@mail.yahoo.com> Message-ID: <518035561.714782.1468187066318.JavaMail.yahoo@mail.yahoo.com> Hi Linda, I've set up three histology labs for animal research: one in an academic setting, one for a pharmaceutical firm, and one for a research foundation. ? 1. Having money to buy the proper equipment is tricky, start with used equipment and make due until you can afford to buy new. ?As long as you buy the equipment from somewhere that ? ? ? either provides a warranty or the equipment is so inexpensive that you don't care if it breaks (as long as it gets you going). 2. Having the investors (or whom ever is supplying the majority of the funding) understand that often times histology labs that are providing a service to researchers do not make money. ? ? ? ? In the early stages they are black pits you pour money into. 3. Building up a clientele requires a lot of advertising and offering low prices (undercutting the competition even if it means you suffer a loss). 4. Have a method of billing, invoicing, and collecting on those invoices can be a challenge. ?Simple bookkeeping software like Quickbooks or FileMaker will help you considerably. ? 5. Write your SOPs as soon as you can. ?Having the methodology of every technique, stain and procedure you will be doing is invaluable. ?When you get around to be able to hire staff ? ? ? ? ? these will be a lifesaver. ?Having established SOPs allow you to make sure your staff also follow the same procedure. ?Reproducability and consistency is a must when establishing a ? ? ? ? histology lab. ?There is no shame in borrowing heavily from SOPs you have come across in the past. ?Rely on colleagues to provide SOPs and pointers. 6. ?Make sure that the place you select for the business allows for proper ventilation of all the reagents and chemicals. ?You want to limit your exposure to the chemicals in the lab to the ? ? ? ? ?maximum point possible. ?Safety should be of the utmost importance in the new lab. Those are just a few things I can think of. ?Most important is to enjoy the ride-it's going to be hard and sometimes frustrating work-but building the business is part of the fun.?Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health On Saturday, July 9, 2016 5:08 PM, Linda via Histonet wrote: Hello Histoland, I am inquiring if anyone has started their own lab to?do histology services for veterinarians?or animal research?tissues? Or anyone who has started a private lab? What are your experiences?? What didn't you expect?? Thank you in advance. Regards, Linda Dee, BGS,HT(ASCP)?Lmdee1 at yahoo.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ewj at pigs.ag Sun Jul 10 23:20:55 2016 From: ewj at pigs.ag (=?UTF-8?B?RS4gV2F5bmUgSm9obnNvbuOAgOacseeos+ajruWNmuWjqw==?=) Date: Mon, 11 Jul 2016 12:20:55 +0800 Subject: [Histonet] Start up Vet/Animal histology laboratory In-Reply-To: <1990575214.1453277.1468108403616.JavaMail.yahoo@mail.yahoo.com> References: <1990575214.1453277.1468108403616.JavaMail.yahoo.ref@mail.yahoo.com> <1990575214.1453277.1468108403616.JavaMail.yahoo@mail.yahoo.com> Message-ID: <5c3e9486-0f66-d95b-a809-a871fd2d7e4d@pigs.ag> We have our own lab in Beijing and we do both of those things. We have a consulting veterinary practice and the main purpose of the lab is to support that veterinary practice. It would be unusual in the USA to have histopath in a vet lab but in China there was no diagnostic laboratory that did any histopathology at all. We have some cooperation with a university and we get some research samples from them. We had some difficulty procuring reagents at first. Fixed samples from the field or from researchers are often in horrible shape. It really is a disaster when samples from expensive and difficult to set up research are ruined by inept collection and poor fixation... We are primarily interested in pigs but we get research samples from mice and chickens sometimes. If there is a good liaison with the researchers it works well. I have a team of people who know how to select and necropsy pigs for diagnosis and they can do a good job. We made a video to show people in the field how to collect and manage diagnostic samples. I knew that personnel was difficult. If you hire and train young men, they will leave and go work for someone else after they think they know what they are doing. If hire young women, they will get married and have babies and can cause many problems. I am tired of hiring people with advanced degrees (MS, PhD). They tend to be lazy and are rather hard to train. Bright young folk who have the willingness to learn are a joy. The surprising thing about a MS or PhD is what it ain't. In the future I am considering hiring taxi cab drivers for everything. They are smart. They are ambitious. They know how to take directions. They are self-starters and independent workers. On 07/10/2016 07:53 AM, Linda wrote: > Hello Histoland, > I am inquiring if anyone has started their own lab to do histology services for veterinarians or animal research tissues? > Or anyone who has started a private lab? > What are your experiences? What didn't you expect? > Thank you in advance. > Regards, > Linda Dee, BGS,HT(ASCP) Lmdee1 at yahoo.com > > From amosbrooks at gmail.com Mon Jul 11 04:51:35 2016 From: amosbrooks at gmail.com (Amos Brooks) Date: Mon, 11 Jul 2016 05:51:35 -0400 Subject: [Histonet] Mucin on PR Message-ID: Hi, I'm asking this for a colleague that is experiencing this problem on formalin fixed paraffin embedded human clinical samples. She is using PR636 from Dako on a Leica Bond platform. Has anyone seen mucin or any cytoplasmic staining with Progesterone receptor antibody? Thanks folks, Amos From SteveM at mcclainlab.com Mon Jul 11 13:46:02 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Mon, 11 Jul 2016 18:46:02 +0000 Subject: [Histonet] Histonet Digest, Vol 152, Issue 9 lab startup In-Reply-To: References: Message-ID: <241F26FE-3B4A-49D4-8F58-86F37DABDFE0@mcclainlab.com> This is a challenging topic. We did it in 2004. You must have deep pockets and committed clients. Even then, it is a hunter-gatherer lifestyle. Run your traps, and live off what you kill. It can be done, but the wind is always in your face and the first 7 years are mostly uphill. Good luck Steve A. McClain, MD > On Jul 11, 2016, at 13:02, "histonet-request at lists.utsouthwestern.edu" wrote: > > Subject: [Histonet] Start up Vet/Animal histology laboratory > Message-ID: > <1990575214.1453277.1468108403616.JavaMail.yahoo at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > Hello Histoland, > I am inquiring if anyone has started their own lab to?do histology services for veterinarians?or animal research?tissues? > Or anyone who has started a private lab? > What are your experiences?? What didn't you expect?? > Thank you in advance. > Regards, > Linda Dee, BGS,HT(ASCP)?Lmdee1 at yahoo.com > From jpiche at wtbyhosp.org Mon Jul 11 14:22:20 2016 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Mon, 11 Jul 2016 19:22:20 +0000 Subject: [Histonet] Cryostat decontamination ANP23410 Message-ID: <631955447A364B45B9458D290563511001077850B3@WIN08-MBX-01.wtbyhosp.org> Hi Everyone, Just a quick question. If you defrost your cryostat to room temperature and wipe it out with 70% alcohol is that considered decontamination? Or does it have to be fumigated with formaldehyde, or have a UV function on board the machine? Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From vapatpxs at yahoo.com Mon Jul 11 20:41:51 2016 From: vapatpxs at yahoo.com (Va Paula Sicurello) Date: Tue, 12 Jul 2016 01:41:51 +0000 (UTC) Subject: [Histonet] UC San Diego Anatomic Pathology Histology Labs is Hiring References: <1475681716.1546521.1468287711637.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1475681716.1546521.1468287711637.JavaMail.yahoo@mail.yahoo.com> Hello, We are hiring a histotechnologist?for our growing histology lab.? UCSD is adding a new hospital and soon our volume will increase.? We are looking for an experienced histotech.? Please go to:?https://jobs.ucsd.edu/bulletin/job.aspx?cat=health&sortby=post&jobnum_in=81586.?Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health From LaurenHegnerSweeney at uga.edu Tue Jul 12 13:14:09 2016 From: LaurenHegnerSweeney at uga.edu (Lauren Sweeney) Date: Tue, 12 Jul 2016 18:14:09 +0000 Subject: [Histonet] bone saw blades Message-ID: Hi all, in our lab we have a Buehler Isomet Low Speed Saw that looks like it is from the 80's. Up until about 7 months ago, we have used the bone saw fairly infrequently. As far as I know, this bone saw wafering blade has never been replaced. We now have a researcher who regularly needs sections of bone cut and the blade is getting much more frequent use, like hundreds of bones per project. My question to you is, how long do these blades stay sharp? Do they ever need sharpening? What is their lifespan? What kind of maintenance do they need? Thanks so much! Lauren From Richard.Cartun at hhchealth.org Tue Jul 12 13:51:47 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 12 Jul 2016 18:51:47 +0000 Subject: [Histonet] C4d IHC in myocardial biopsy Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E95354751@HHCEXCHMB03.hhcsystem.org> Are any of you an expert with C4d IHC in myocardial biopsy tissue? We had a specimen this week that showed a pattern of immunoreactivity that I am not familiar with and I need to find out if this represents a specific disease process. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From RPHAVICE at geisinger.edu Tue Jul 12 13:51:48 2016 From: RPHAVICE at geisinger.edu (Havice, Rebecca P.) Date: Tue, 12 Jul 2016 18:51:48 +0000 Subject: [Histonet] volunteer in Hati In-Reply-To: References: Message-ID: <95bf24f0508448efbb598e2708deed26@LOFEXMBX106W12V.geisinger.edu> If you want a chance to pass your knowledge of histology forward, consider volunteering in Cap Hatien Haiti histology project. Spend two weeks helping to complete the newly established histology department at Justinien Hospital. This is a much underserved area. For more information contact Vincent DeGennaro Jr. at vincedegennaro at gmail.com Rebecca Havice SCT, HT(ASCP) Technical Specialist (Cytology) Geisinger-Lewistown Hospital 400 Highland Ave. Lewistown, PA 17044 717 242 7069 rphavice at geisinger.edu ________________________________________ From: histonet-request at lists.utsouthwestern.edu [histonet-request at lists.utsouthwestern.edu] Sent: Tuesday, July 12, 2016 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [External] Histonet Digest, Vol 152, Issue 10 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7crphavice%40geisinger.edu%7c483ff9ac5f184078f09c08d3aa760d12%7c37d46c567c664402a16055c2313b910d%7c0&sdata=oXYAW%2b7IpeAszlrwFMSrWii9rYqJ39dM%2bO%2bUjR%2fKyiY%3d or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Histonet Digest, Vol 152, Issue 9 lab startup (Steve McClain) 2. Cryostat decontamination ANP23410 (Piche, Jessica) 3. UC San Diego Anatomic Pathology Histology Labs is Hiring (Va Paula Sicurello) ---------------------------------------------------------------------- Message: 1 Date: Mon, 11 Jul 2016 18:46:02 +0000 From: Steve McClain To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Histonet Digest, Vol 152, Issue 9 lab startup Message-ID: <241F26FE-3B4A-49D4-8F58-86F37DABDFE0 at mcclainlab.com> Content-Type: text/plain; charset="us-ascii" This is a challenging topic. We did it in 2004. You must have deep pockets and committed clients. Even then, it is a hunter-gatherer lifestyle. Run your traps, and live off what you kill. It can be done, but the wind is always in your face and the first 7 years are mostly uphill. Good luck Steve A. McClain, MD > On Jul 11, 2016, at 13:02, "histonet-request at lists.utsouthwestern.edu" wrote: > > Subject: [Histonet] Start up Vet/Animal histology laboratory > Message-ID: > <1990575214.1453277.1468108403616.JavaMail.yahoo at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > Hello Histoland, > I am inquiring if anyone has started their own lab to?do histology services for veterinarians?or animal research?tissues? > Or anyone who has started a private lab? > What are your experiences?? What didn't you expect?? > Thank you in advance. > Regards, > Linda Dee, BGS,HT(ASCP)?Lmdee1 at yahoo.com > ------------------------------ Message: 2 Date: Mon, 11 Jul 2016 19:22:20 +0000 From: "Piche, Jessica" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Cryostat decontamination ANP23410 Message-ID: <631955447A364B45B9458D290563511001077850B3 at WIN08-MBX-01.wtbyhosp.org> Content-Type: text/plain; charset="us-ascii" Hi Everyone, Just a quick question. If you defrost your cryostat to room temperature and wipe it out with 70% alcohol is that considered decontamination? Or does it have to be fumigated with formaldehyde, or have a UV function on board the machine? Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital ------------------------------ Message: 3 Date: Tue, 12 Jul 2016 01:41:51 +0000 (UTC) From: Va Paula Sicurello To: Histonet List Subject: [Histonet] UC San Diego Anatomic Pathology Histology Labs is Hiring Message-ID: <1475681716.1546521.1468287711637.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Hello, We are hiring a histotechnologist?for our growing histology lab.? UCSD is adding a new hospital and soon our volume will increase.? We are looking for an experienced histotech.? Please go to:?https://jobs.ucsd.edu/bulletin/job.aspx?cat=health&sortby=post&jobnum_in=81586.?Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7crphavice%40geisinger.edu%7c483ff9ac5f184078f09c08d3aa760d12%7c37d46c567c664402a16055c2313b910d%7c0&sdata=oXYAW%2b7IpeAszlrwFMSrWii9rYqJ39dM%2bO%2bUjR%2fKyiY%3d ------------------------------ End of Histonet Digest, Vol 152, Issue 10 ***************************************** IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From relia1 at earthlink.net Wed Jul 13 11:41:01 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 13 Jul 2016 12:41:01 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin 7-13-2016 It's A Sizzlin Hot Summer!!! Message-ID: <204001d1dd25$57233a70$0569af50$@earthlink.net> Hi Histonetters!! I hope you are having a great day. Are you keeping cool on these hot summer days? It?s Summertime and it?s Sizzlin? Hot and It's not just the temps!! I have some great new opportunities to tell you about: o I have full time positions with the best employers in the country. o Competitive salaries, great benefits & relocation assistance. o Positions for Experienced Certified and Uncertified Techs!! o My clients are ready to interview and hire today!! They are waiting to hear from me about histotechs like you and your friends!! Here is a list of the Sizzlin Summer Stuff! Modesto, CA IHC Histotech Austin, TX Histology Tech Tyler, TX Senior Histology Tech Flagstaff, AZ Histology Tech II Nashville, TN Histotechnician Hammond, IN Histotech If you have any questions or know someone who might be interested please contact me. I can be reached toll free at the office at 866-607-3542, email me at relia1 at earthlink.net or call/text me on my cell at 407-353-5070. I would love to tell you about these opportunities or write another referral check like I did today if you have a friend who might be interested. I hope to hear from you soon. In the meantime grab some shade and a cool drink! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From LisaKennedy at catholichealth.net Thu Jul 14 05:18:12 2016 From: LisaKennedy at catholichealth.net (Kennedy, Lisa) Date: Thu, 14 Jul 2016 10:18:12 +0000 Subject: [Histonet] Cleaning Tissue Molds Message-ID: <517C2A781E654047A5EC98D17390F9F25D939672@CHIEX003.CHI.catholichealth.net> Dear Fellow Histo Techs, What is the BEST practice for cleaning the paraffin block tissue molds? We do not want to use our processor due to its age and wear and tear and frequent replacement of cellanoids when we clean them via the processor. Thanks so much for your help! In advance. Sincerely, Lisa Kennedy, HT (ASCP) This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From Valerie.Hannen at parrishmed.com Thu Jul 14 06:56:43 2016 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Thu, 14 Jul 2016 07:56:43 -0400 Subject: [Histonet] Cleaning Tissue Molds In-Reply-To: <517C2A781E654047A5EC98D17390F9F25D939672@CHIEX003.CHI.catholichealth.net> References: <517C2A781E654047A5EC98D17390F9F25D939672@CHIEX003.CHI.catholichealth.net> Message-ID: <450B7A81EDA0C54E97C53D60F00776C323E455E60B@isexstore03> We soak our molds in Xylene for @ 2hrs, then soak in 100% alcohol for @ 30 minutes( with occasional stirring to clear the xylene), then rinse in H2O, let dry(laid out) and spray with the mold release. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Kennedy, Lisa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 14, 2016 6:18 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cleaning Tissue Molds Dear Fellow Histo Techs, What is the BEST practice for cleaning the paraffin block tissue molds? We do not want to use our processor due to its age and wear and tear and frequent replacement of cellanoids when we clean them via the processor. Thanks so much for your help! In advance. Sincerely, Lisa Kennedy, HT (ASCP) This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From idimitro at mun.ca Thu Jul 14 08:35:22 2016 From: idimitro at mun.ca (idimitro at mun.ca) Date: Thu, 14 Jul 2016 13:35:22 +0000 Subject: [Histonet] Cleaning Tissue Molds In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C323E455E60B@isexstore03> References: <517C2A781E654047A5EC98D17390F9F25D939672@CHIEX003.CHI.catholichealth.net> <450B7A81EDA0C54E97C53D60F00776C323E455E60B@isexstore03> Message-ID: <14C3108E8B98EF43B3EDAE58336700340202495965@exchange.med.mun.ca> We used to do that until 5 years ago, when we started looking in cleaning the molds without the use of Xylene. We found that we don't need to clan them, we turn them down on a metal tray covered with paper towel and put them in our 60 degree C oven overnight. That gets rid of the wax left on the molds. Then the next morning we spray them with mold release and they are ready for use. Now, bear in mind we do only research, so I don't know if you can do this in a hospital, there may be possible contamination or other issues, but it perfect solution for us. I. Dimitrova, MLT, LHP, B.Tech., M.Sc. Histology Supervisor Faculty of Medicine Memorial University of Newfoundland St. John's, NL Canada -----Original Message----- From: Hannen, Valerie via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: July-14-16 9:27 AM To: 'Kennedy, Lisa' Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Cleaning Tissue Molds We soak our molds in Xylene for @ 2hrs, then soak in 100% alcohol for @ 30 minutes( with occasional stirring to clear the xylene), then rinse in H2O, let dry(laid out) and spray with the mold release. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Kennedy, Lisa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 14, 2016 6:18 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cleaning Tissue Molds Dear Fellow Histo Techs, What is the BEST practice for cleaning the paraffin block tissue molds? We do not want to use our processor due to its age and wear and tear and frequent replacement of cellanoids when we clean them via the processor. Thanks so much for your help! In advance. Sincerely, Lisa Kennedy, HT (ASCP) This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Thu Jul 14 09:11:18 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 14 Jul 2016 14:11:18 +0000 (UTC) Subject: [Histonet] Cleaning Tissue Molds In-Reply-To: <517C2A781E654047A5EC98D17390F9F25D939672@CHIEX003.CHI.catholichealth.net> References: <517C2A781E654047A5EC98D17390F9F25D939672@CHIEX003.CHI.catholichealth.net> Message-ID: <1673797881.3661474.1468505478591.JavaMail.yahoo@mail.yahoo.com> Place your molds in a 2% dishwasher soap boiling solution for 5 minutes ? was in running water for 5 minutes ? dry in a convection oven at 60?C for 10 minutes and your molds will be ready to use.As a "release" solution use a mixture 1:1 of 2-propanol and mineral oil (light weight).Ren? On Thursday, July 14, 2016 6:34 AM, "Kennedy, Lisa via Histonet" wrote: Dear Fellow Histo Techs, What is the BEST practice for cleaning the paraffin block tissue molds?? We do not want to use our processor due to its age and wear and tear and frequent replacement of cellanoids when we clean them via the processor. Thanks so much for your help! In advance. Sincerely, Lisa Kennedy, HT (ASCP) This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Thu Jul 14 12:20:40 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 14 Jul 2016 17:20:40 +0000 Subject: [Histonet] cleaning molds Message-ID: <48E053DDF6CE074DB6A7414BA05403F8080BA6@HRHEX02-HOS.holyredeemer.local> We used to put them in a large stainless steel basin, with a little bit of Alconox brand detergent. Fill the basin with hot water and bring to a boil on a hotplate. Turn it off, let the water cool for a couple of hours and all the paraffin would rise to the top and solidify. Just pick the paraffin off the top and discard, then rinse the soapy clean molds in clean water, drain and dry. No nasty chemical soaks. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 *************************************** From nlinke at sbch.org Thu Jul 14 12:28:29 2016 From: nlinke at sbch.org (Noelle Linke) Date: Thu, 14 Jul 2016 17:28:29 +0000 Subject: [Histonet] looking for Skip Brown Message-ID: Hi all, I am looking for contact info for Skip Brown, does anyone know how to reach him? Skip, if you are on here, please reach out to me! Thank you, No?lle No?lle Linke, MS, HTL(ASCP) QIHC Manager, Anatomic Pathology Pacific Diagnostic Laboratories nlinke at sbch.org Phone: (805) 324-9814 Fax: (805) 696-6433 ________________________________ CH Disclaimer: This electronic mail message is intended exclusively for the individual or entity to which it is addressed. This message, together with any attachment, may contain confidential and privileged information. Any views, opinions or conclusions expressed in this message are those of the individual sender and do not necessarily reflect the views of Cottage Health, its subsidiaries or affiliates. This document may also contain information covered under the Health Insurance Portability and Accountability Act (HIPAA, PL 104-191) and implementing regulations and must be protected in accordance with those provisions. Re-disclosure without patient consent or as otherwise permitted by law is prohibited. Any unauthorized review, retransmission, use, printing, copying, retention, disclosure, distribution or the taking of any action in reliance upon this information by persons or entities other than the intended recipient is strictly prohibited and may be unlawful. If you have received this message in error, please immediately advise the sender by reply email message to the sender and delete all copies of this message from your system without copying. ________________________________ From craigak12 at gmail.com Thu Jul 14 13:31:36 2016 From: craigak12 at gmail.com (J B) Date: Thu, 14 Jul 2016 18:31:36 +0000 Subject: [Histonet] Slide cost: Message-ID: I would like to know if anyone can share cost or how they determine cost for additional services. We have a client that wants us to prepare slides for a research project. Cutting unstained slides at various thicknesses. This is going to be a time consuming project and I would like to make a high return, not sure where to start. Please advise. Thank you, JB From TNMayer at mdanderson.org Thu Jul 14 14:12:01 2016 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Thu, 14 Jul 2016 19:12:01 +0000 Subject: [Histonet] Cleaning Tissue Molds (Kennedy, Lisa Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8828217B5C@D1PWPEXMBX05.mdanderson.edu> Lisa, I have used all of the previously mentioned methods for cleaning molds. It really depends on the facilities that you have access to. Boiling in hot soapy water is great for deep cleaning, but you may not have enough pots or beakers to clean them all at once. Xylene, followed by 100% is good, but you may not have it in the budget to use fresh xylene and forget to save some used. Sometimes having a container big enough to do this is also a challenge. Spraying them down with mold release after air drying, but then you have to remember to allow them to dry. Placing them in the processor can clog the lines, but if you place them in the oven first to drain and only do a few at a time that can help. We have our students boil them and then dry them in the oven. Weekend dry in the oven, and on Monday am they shake them up and put them away. Some techs don't like the residue of mold release. It doesn't bother me though. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 The information contained in the e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. Message: 1 Date: Thu, 14 Jul 2016 10:18:12 +0000 From: "Kennedy, Lisa" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Cleaning Tissue Molds Message-ID: <517C2A781E654047A5EC98D17390F9F25D939672 at CHIEX003.CHI.catholichealth.net> Content-Type: text/plain; charset="us-ascii" Dear Fellow Histo Techs, What is the BEST practice for cleaning the paraffin block tissue molds? We do not want to use our processor due to its age and wear and tear and frequent replacement of cellanoids when we clean them via the processor. Thanks so much for your help! In advance. Sincerely, Lisa Kennedy, HT (ASCP) This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ------------------------------ Message: 2 Date: Thu, 14 Jul 2016 07:56:43 -0400 From: "Hannen, Valerie" To: "'Kennedy, Lisa'" Cc: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Cleaning Tissue Molds Message-ID: <450B7A81EDA0C54E97C53D60F00776C323E455E60B at isexstore03> Content-Type: text/plain; charset="us-ascii" We soak our molds in Xylene for @ 2hrs, then soak in 100% alcohol for @ 30 minutes( with occasional stirring to clear the xylene), then rinse in H2O, let dry(laid out) and spray with the mold release. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com Message: 3 Date: Thu, 14 Jul 2016 13:35:22 +0000 From: To: , Cc: Subject: Re: [Histonet] Cleaning Tissue Molds Message-ID: <14C3108E8B98EF43B3EDAE58336700340202495965 at exchange.med.mun.ca> Content-Type: text/plain; charset="us-ascii" We used to do that until 5 years ago, when we started looking in cleaning the molds without the use of Xylene. We found that we don't need to clan them, we turn them down on a metal tray covered with paper towel and put them in our 60 degree C oven overnight. That gets rid of the wax left on the molds. Then the next morning we spray them with mold release and they are ready for use. Now, bear in mind we do only research, so I don't know if you can do this in a hospital, there may be possible contamination or other issues, but it perfect solution for us. I. Dimitrova, MLT, LHP, B.Tech., M.Sc. Histology Supervisor Faculty of Medicine Memorial University of Newfoundland St. John's, NL Canada -----Original Message----- From: Hannen, Valerie via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: July-14-16 9:27 AM To: 'Kennedy, Lisa' Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Cleaning Tissue Molds We soak our molds in Xylene for @ 2hrs, then soak in 100% alcohol for @ 30 minutes( with occasional stirring to clear the xylene), then rinse in H2O, let dry(laid out) and spray with the mold release. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Kennedy, Lisa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 14, 2016 6:18 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cleaning Tissue Molds Dear Fellow Histo Techs, What is the BEST practice for cleaning the paraffin block tissue molds? We do not want to use our processor due to its age and wear and tear and frequent replacement of cellanoids when we clean them via the processor. Thanks so much for your help! In advance. Sincerely, Lisa Kennedy, HT (ASCP) Message: 4 Date: Thu, 14 Jul 2016 14:11:18 +0000 (UTC) From: Rene J Buesa To: "Kennedy, Lisa" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Cleaning Tissue Molds Message-ID: <1673797881.3661474.1468505478591.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Place your molds in a 2% dishwasher soap boiling solution for 5 minutes ? was in running water for 5 minutes ? dry in a convection oven at 60?C for 10 minutes and your molds will be ready to use.As a "release" solution use a mixture 1:1 of 2-propanol and mineral oil (light weight).Ren? On Thursday, July 14, 2016 6:34 AM, "Kennedy, Lisa via Histonet" wrote: Dear Fellow Histo Techs, What is the BEST practice for cleaning the paraffin block tissue molds?? We do not want to use our processor due to its age and wear and tear and frequent replacement of cellanoids when we clean them via the processor. Thanks so much for your help! In advance. Sincerely, Lisa Kennedy, HT (ASCP) This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From JMacDonald at mtsac.edu Fri Jul 15 01:19:56 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Thu, 14 Jul 2016 23:19:56 -0700 Subject: [Histonet] Cleaning Tissue Molds In-Reply-To: <517C2A781E654047A5EC98D17390F9F25D939672@CHIEX003.CHI.catholichealth.net> References: <517C2A781E654047A5EC98D17390F9F25D939672@CHIEX003.CHI.catholichealth.net> Message-ID: From LisaKennedy at catholichealth.net Fri Jul 15 08:04:44 2016 From: LisaKennedy at catholichealth.net (Kennedy, Lisa) Date: Fri, 15 Jul 2016 13:04:44 +0000 Subject: [Histonet] Cleaning Tissue Molds Message-ID: <517C2A781E654047A5EC98D17390F9F25D9396E6@CHIEX003.CHI.catholichealth.net> Thanks to everyone who submitted their best practices to me in regards to cleaning the tissue block molds. I appreciate all of your great ideas! Thanks again, Lisa This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From halsteaj at ohsu.edu Fri Jul 15 10:33:58 2016 From: halsteaj at ohsu.edu (Jeff Halstead) Date: Fri, 15 Jul 2016 15:33:58 +0000 Subject: [Histonet] dye in processor Message-ID: <398DA4E9BECCE44288904391038A80DE45C1D11A@EXMB03.ohsu.edu> Hi all some time ago info on the list discussed placing eosin or other dyes in the processor and I was wondering what everyone is actually using. Heard safranin o was a good idea . any thoughts. Thanx jeff From Megan.Dishop at childrensmn.org Fri Jul 15 10:49:32 2016 From: Megan.Dishop at childrensmn.org (Megan Dishop) Date: Fri, 15 Jul 2016 10:49:32 -0500 Subject: [Histonet] lifting sections from slides to re-stain? In-Reply-To: <398DA4E9BECCE44288904391038A80DE45C1D11A@EXMB03.ohsu.edu> References: <398DA4E9BECCE44288904391038A80DE45C1D11A@EXMB03.ohsu.edu> Message-ID: <5788BFBC.D202.00E3.1@childrensmn.org> Hi Histonet, Does anyone have a procedure or practical tips for lifting and transferring individual H&E stained sections from a stained slide to a new slide to allow destaining and restaining of selected pieces of tissue? Thanks in advance for your help, Megan Megan K. Dishop MD Medical Director, Pediatric Anatomic Pathology Children's Hospitals and Clinics of Minnesota Laboratories 2525 Chicago Ave S. MS32-B600, Minneapolis, MN 55404 USA Phone: 612-813-6521 Fax: 612-813-7721 Email: megan.dishop at childrensMN.org Adjoint Professor of Pediatrics, University of Colorado School of Medicine Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. From casey.berridge at unitybiotechnology.com Fri Jul 15 12:09:22 2016 From: casey.berridge at unitybiotechnology.com (Casey Berridge) Date: Fri, 15 Jul 2016 10:09:22 -0700 Subject: [Histonet] GMA Tissue Embedding and Protocols Message-ID: Hi all, I am going to be exploring some tissue embedding using Glycolmethacrylate (GMA), as I read that it can preserve enzyme function in some cases and potentially can be used for IHC. However, information is a bit scant and can be contradictory at times, to say the least. If anyone has any experience doing any histological or IHC staining on GMA embedded tissue, or knows of anywhere I could get protocols from or any papers that are a bit more recent (I'm mostly finding 90s, early 00s), pleas let me know, I would really appreciate any resources I can find! -- Casey Berridge From tkngflght at yahoo.com Fri Jul 15 23:52:56 2016 From: tkngflght at yahoo.com (Cheryl) Date: Sat, 16 Jul 2016 04:52:56 +0000 (UTC) Subject: [Histonet] Position in Eastern PA In-Reply-To: <1977588359.3442994.1468526835155.JavaMail.yahoo@mail.yahoo.com> References: <1977588359.3442994.1468526835155.JavaMail.yahoo.ref@mail.yahoo.com> <1977588359.3442994.1468526835155.JavaMail.yahoo@mail.yahoo.com> Message-ID: <172889696.101652.1468644776438.JavaMail.yahoo@mail.yahoo.com> ? Hi Guys- Looking for a registered tech who is eligible to gross.? Solo tech lab--perfect full time job for an independent soul.? Physician group for a derm practice.?Potential to cross train for Mohs (depending on the candidate).? ?North of Philadelphia yet within reach of Allentown, and parts of Delaware and New Jersey. Interested-- give me a holler -- happy to share more info. Thank you!! ?Cheryl Kerry, HT(ASCP) Full Staff Inc. ? admin at fullstaff.org?800.756.3309 Phone & Fax 281.883.7704 Cell and Text https://www.facebook.com/TheHistologyCompany/ I work a day job as a histotech-- please leave a message and I WILL return your call! From rsrichmond at gmail.com Sat Jul 16 10:11:08 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 16 Jul 2016 11:11:08 -0400 Subject: [Histonet] dye in processor Message-ID: Jeff Halstead at Oregon Health & Science University asks: "Some time ago info on the list discussed placing eosin or other dyes in the processor and I was wondering what everyone is actually using. Heard safranin o was a good idea . any thoughts?" Safranin O - the solution your microbiology people use for Gram staining works quite well - can be used to mark small pieces of tissue before processing. It stains more densely than eosin, and unlike eosin it isn't fluorescent. I worked in a lab that used this quite successfully. They placed the small specimens on those blue pads before marking them with a drop of dye, processing through xylene (or aliphatic substitute - I don't remember) on a truly ancient Fisher processor. Both they and I liked the results. Bob Richmond Samurai Pathologist Maryville TN From rsrichmond at gmail.com Sat Jul 16 10:15:58 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 16 Jul 2016 11:15:58 -0400 Subject: [Histonet] Histonet Digest, Vol 152, Issue 13 In-Reply-To: References: Message-ID: Jeff Halstead at Oregon Health & Science University asks: "Some time ago info on the list discussed placing eosin or other dyes in the processor and I was wondering what everyone is actually using. Heard safranin o was a good idea . any thoughts?" Some time ago I worked in a lab that used safranin O to mark small tissue specimens, putting them on blue foam pads, marking them with a drop of dye, and processing through xylene (or aliphatic substitute - I don't remember) with an ancient Fisher processor. Both they and I were happy with the results. They used the safranin O solution their microbiologists used in the Gram stain. Bob Richmond Samurai Pathologist Maryville TN From dblackburn2000 at yahoo.com Sat Jul 16 14:51:23 2016 From: dblackburn2000 at yahoo.com (daniel blackburn) Date: Sat, 16 Jul 2016 19:51:23 +0000 (UTC) Subject: [Histonet] plastic sectioning advice requested References: <682357368.319688.1468698683058.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <682357368.319688.1468698683058.JavaMail.yahoo@mail.yahoo.com> My lab hopes to get into plastic sectioning. We need to be able to process tissue pieces as large and thick as possible, but see that the largest embedding molds for JB4 are only 13x19mm by 5mm deep. We have two questions: (1) Do any of the available media (plastic or resins) allow one to embed and section large pieces (for example eggs with dimensions of 2 cm or larger)? (2) Is a special microtome (such as a retracting microtome) needed? Our reason for considering plastic is that we must section yolk, which splits out of standard paraffin during sectioning. Any advice is appreciated. -- Daniel Blackburn, Trinity College From SteveM at mcclainlab.com Mon Jul 18 06:19:17 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Mon, 18 Jul 2016 11:19:17 +0000 Subject: [Histonet] 360 video of specimens In-Reply-To: References: Message-ID: The next 3 weeks we are turning our lab cameras and our specimens toward making a 360 photographic orbits around specimens to create a 360 stitched image or video. If you know any pathologists who have tried 360 imaging around a specimen, kindly send me their name. I have not seen any prior art. Steve A. McClain, MD > On Jul 17, 2016, at 13:23, "histonet-request at lists.utsouthwestern.edu" wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. plastic sectioning advice requested (daniel blackburn) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 16 Jul 2016 19:51:23 +0000 (UTC) > From: daniel blackburn > To: > Subject: [Histonet] plastic sectioning advice requested > Message-ID: > <682357368.319688.1468698683058.JavaMail.yahoo at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > My lab hopes to get into plastic sectioning. We need to be able to process tissue pieces as large and thick as possible, but see that the largest embedding molds for JB4 are only 13x19mm by 5mm deep. We have two questions: (1) Do any of the available media (plastic or resins) allow one to embed and section large pieces (for example eggs with dimensions of 2 cm or larger)? (2) Is a special microtome (such as a retracting microtome) needed? Our reason for considering plastic is that we must section yolk, which splits out of standard paraffin during sectioning. Any advice is appreciated. -- Daniel Blackburn, Trinity College > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 152, Issue 15 > ***************************************** From annamhuntley at gmail.com Mon Jul 18 07:40:34 2016 From: annamhuntley at gmail.com (Anna Huntley Coffey) Date: Mon, 18 Jul 2016 08:40:34 -0400 Subject: [Histonet] Santa Cruz Antibodies Message-ID: Hello Histonet, I'm hoping someone will be able to clear something up for me. I've seen several articles in the news recently regarding the revocation of Santa Cruz' animal dealer license, the closing of their research lab, and no longer being allowed to sell antibodies past December 31 of this year. I thought I had a good handle of what to expect, but my colleague just received an email the other day from Santa Cruz that they are expanding a bunch of products (monoclonal antibodies, RNAi, biochemicals and CRISPR). Did I miss something? Also, if you have any information, it would be great if you could include an official source. Thanks, Anna From tilycyn at auburn.edu Mon Jul 18 11:27:16 2016 From: tilycyn at auburn.edu (Cynthia Hutchinson) Date: Mon, 18 Jul 2016 16:27:16 +0000 Subject: [Histonet] FTE at Auburn University Coll of Vet Med Message-ID: Hello Again, Auburn University College of Veterinary Medicine has a full time position for a histotech. Hours are M-F , 8-5. Limited holiday schedule. HT/HTL preferred, but those eligible to sit for the exam soon will also be considered. There is a glitch in the university application system so please contact me directly if interested. To see the full job description got to : https://www.auemployment.com/applicants/jsp/shared/frameset/Frameset.jsp?time=1468859129811 Tech I/II, Histological 26456 Pathobiology Cynthia Tily Hutchinson Rsch Asst IV Pathobiology 166 Greene Hall Coll of Vet Med Auburn Univ 36849 (334)844 7020 From jvickroy at SpringfieldClinic.com Mon Jul 18 11:33:05 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Mon, 18 Jul 2016 16:33:05 +0000 Subject: [Histonet] Prefilled formalin containers Message-ID: <9B1A1501A800064397369BD8072E6BCA06585D49@E2K10DB.springfieldclinic.com> This is one of the things that comes up every couple of years. Leaky prefilled formalin containers. I know we have all dealt with nurses or physicians that can't put the lid on correctly or put the label on the threads of the containers. Some of the new designs have a "clicking lid" when they are supposed to be sealed. My experience with the "clicking lids" are that some vendors have lids that the "clicker" breaks off as soon as you unscrew it so obviously it doesn't help when putting the lid back on. Another vendor that has the "clicking lid" does not have the "clicker" break off when you unscrew and screw but the containers still leak. It seems to me that someone could come up with accost-effective prefilled formalin container that does not leak (of course provided the lids was put on straight). I would be interested in other's experiences with this elemental yet extremely annoying issue. It seems like if we can make new automated electronic instruments someone might be able to make affordable container that doesn't leak. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From rsrichmond at gmail.com Mon Jul 18 12:40:17 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Mon, 18 Jul 2016 13:40:17 -0400 Subject: [Histonet] 360 video of specimens Message-ID: Steve A. McClain, MD asks: >>The next 3 weeks we are turning our lab cameras and our specimens toward making a 360 photographic orbits around specimens to create a 360 stitched image or video. If you know any pathologists who have tried 360 imaging around a specimen, kindly send me their name. I have not seen any prior art.<< What a cool idea! I've yet to work on a pathology service that permitted gross photography, or graphics in reports. What are you doing - putting the specimen on a turntable? Bob Richmond Samurai Pathologist Maryville TN From Richard.Cartun at hhchealth.org Mon Jul 18 15:20:30 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 18 Jul 2016 20:20:30 +0000 Subject: [Histonet] Low temperature freezer Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E9535E87E@HHCEXCHMB03.hhcsystem.org> Our ancient "SoLow" -80 degree C. freezer died over the weekend. What do you recommend as a replacement? Do you prefer a chest vs. stand-up? We use it primarily for tissue storage. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From CDavis at che-east.org Tue Jul 19 08:31:51 2016 From: CDavis at che-east.org (Cassie P. Davis) Date: Tue, 19 Jul 2016 13:31:51 +0000 Subject: [Histonet] Plastic embedding and Microtomy Message-ID: <5C815EADE724D14AA0CC8F037C4185F079B32D87@SB01MSTMBX13.sb.trinity-health.org> Hi Daniel, I haven't seen plastics embedding and microtomy since the mid 90's. When I was at CCHS in Newark, DE we used to do our bone marrow biopsies in them. At that time there were molds big enough to embed a penny in. Yes, you need a different microtome to section these specimens. We had a glass knife micotome. We had to score and break glass knives at least once a week. This is a little dangerous and quite tempermental. The edge on the glass knife didn't seem to last more than 5 days and not everybody was good at making the glass knives. We adventually, reverted back to paraffin a few years before I left ('99). If I remember correctly, IHC does not perform well on the "plastics" specimens which is why we would typically process and embed in paraffin. We would routinely perform trichrome,PAS and retic on the plastic sections as well as H&E. I wish I could offer you more information than this. Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From LRaff at uropartners.com Tue Jul 19 09:00:08 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 19 Jul 2016 14:00:08 +0000 Subject: [Histonet] Blog Post (with Explanation) Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10EB48B3@COLOEXCH01.uropartners.local> The linked blog is not about lab/histology, but a histonetter Downsize, Maybe subscriber who read it suggested that it could perhaps be useful for h. pylori and worth a post-so here goes. http://www.chicagonow.com/downsize-maybe/2016/07/we-take-a-shine-to-yinmn-its-the-new-blue/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From lblazek at digestivespecialists.com Tue Jul 19 11:35:00 2016 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Tue, 19 Jul 2016 12:35:00 -0400 Subject: [Histonet] expiration dates Message-ID: <5A2BD13465E061429D6455C8D6B40E39177794BA8E@IBMB7Exchange.digestivespecialists.com> Does anyone see an expiration date printed on their can of Freeze Spray? I was told by the company I purchase mine from that it was not needed because it was "not a medical device". Thanks Linda From Lynne.Bell at cvmc.org Tue Jul 19 11:52:10 2016 From: Lynne.Bell at cvmc.org (Bell, Lynne) Date: Tue, 19 Jul 2016 16:52:10 +0000 Subject: [Histonet] expiration dates In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39177794BA8E@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E39177794BA8E@IBMB7Exchange.digestivespecialists.com> Message-ID: My can of Fisherbrand Freeze'it does not have an expiration date on it but I have Cytocool II from Richard-Allan Scientific does have one (it's printed on the bottom of the can). Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 (802)371-4923 From liz at premierlab.com Tue Jul 19 11:58:45 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Tue, 19 Jul 2016 10:58:45 -0600 Subject: [Histonet] expiration dates In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39177794BA8E@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E39177794BA8E@IBMB7Exchange.digestivespecialists.com> Message-ID: <14E2C6176416974295479C64A11CB9AE02BED535B3F8@SBS2K8.premierlab.local> Linda We have in our reagent policy that any chemical that does not come with an expiration date - we assign it a 5 year from receipt expiration date. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Blazek, Linda via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, July 19, 2016 10:35 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] expiration dates Does anyone see an expiration date printed on their can of Freeze Spray? I was told by the company I purchase mine from that it was not needed because it was "not a medical device". Thanks Linda _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kkienitz at orclinic.com Tue Jul 19 12:39:52 2016 From: kkienitz at orclinic.com (Kienitz, Kari) Date: Tue, 19 Jul 2016 17:39:52 +0000 Subject: [Histonet] Prefilled formalin containers In-Reply-To: <9B1A1501A800064397369BD8072E6BCA06585D49@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA06585D49@E2K10DB.springfieldclinic.com> Message-ID: I have had this annoying experience a few times over the last 20+ years. It seems to me the breakdown is with quality control at the manufacturer. Most recently, 1 out of 5 containers came back to the lab leaking. I put pressure on my vendor, who put pressure on his vendor. Unfortunatly, his vendor really didn't take the situation seriously. Even after explaining the dangers to lab personnel as well as patients being exposed to formalin they did nothing. I feel fortunate the histology supply vendor I deal with values our business. He went and found a new vendor/manufacturer that has supplied us with containers that don't leak and so far they understand the health hazzard and the annoyance of what to many may seem trivial. Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz at orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: Vickroy, James via Histonet [histonet at lists.utsouthwestern.edu] Sent: Monday, July 18, 2016 9:33 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Prefilled formalin containers This is one of the things that comes up every couple of years. Leaky prefilled formalin containers. I know we have all dealt with nurses or physicians that can't put the lid on correctly or put the label on the threads of the containers. Some of the new designs have a "clicking lid" when they are supposed to be sealed. My experience with the "clicking lids" are that some vendors have lids that the "clicker" breaks off as soon as you unscrew it so obviously it doesn't help when putting the lid back on. Another vendor that has the "clicking lid" does not have the "clicker" break off when you unscrew and screw but the containers still leak. It seems to me that someone could come up with accost-effective prefilled formalin container that does not leak (of course provided the lids was put on straight). I would be interested in other's experiences with this elemental yet extremely annoying issue. It seems like if we can make new automated electronic instruments someone might be able to make affordable container that doesn't leak. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thoward at unm.edu Tue Jul 19 13:29:39 2016 From: thoward at unm.edu (Tamara Howard) Date: Tue, 19 Jul 2016 18:29:39 +0000 Subject: [Histonet] Low temperature freezer Message-ID: Richard - We learned last year (the hard way) that chest freezers are more likely to survive a flood than are uprights, if that is any help! The compressors tend to be near the top of the unit in the chest freezers. I think the footprint may be the main deciding factor for many labs; we just bought an upright to replace one that the flood destroyed, only because we don't have space to put in a chest freezer with the same storage capacity. Tamara ................................................... Tamara Howard Dept. of Cell Biology & Physiology University of New Mexico Albuquerque, NM From gayle.callis at bresnan.net Tue Jul 19 16:19:19 2016 From: gayle.callis at bresnan.net (Gayle Callis) Date: Tue, 19 Jul 2016 16:19:19 -0500 Subject: [Histonet] glycol methylacrylate GMA enzyme and IHC Message-ID: <002801d1e203$36e2af60$a4a80e20$@bresnan.net> You wrote: Hi all, I am going to be exploring some tissue embedding using Glycolmethacrylate (GMA), as I read that it can preserve enzyme function in some cases and potentially can be used for IHC. However, information is a bit scant and can be contradictory at times, to say the least. If anyone has any experience doing any histological or IHC staining on GMA embedded tissue, or knows of anywhere I could get protocols from or any papers that are a bit more recent (I'm mostly finding 90s, early 00s), pleas let me know, I would really appreciate any resources I can find! -- Casey Berridge **************************************************************************** ************************************** GMA references from the 80?s and 90?s pretty much remain current for GMA. Be sure to do a literature search in J Histochem Cytochem (JHC.org) to see if there are more recent publications. Google Scholar was not turning up much for recent years. We worked with GMA for many years but never for IHC. There are many considerations when using GMA both good and bad with emphasis on the negative side of things from my point of view. We had success when studying some single cell protozoa, including Cryptosporidia in a research setting. There is one publication in the old Stain Technology, now Biotechnic & Histochemistry using GMA for successful enzyme staining. Namba M et al. Improvement in histochemical demonstration of esterase in glycol methacrylate tissue sections by cold temperature embedding in glycol methacrylate. 1983 58(4):207. Several things about GMA. Requires a fume hood in order to work with toxic and carcinogenic chemicals. Glycol methacrylate is sensitizing and several colleagues are so allergic to fumes after working with this plastic over several years, they can?t be in the same room where GMA is being worked with. Double gloving is advisable, and wearing safety glasses is a must since the sections are small and can fly into an eye (know of this happening) which is not a good situation. There should be no skin contact with the plastic, nor breathing the n, n, di methylaniline, a carcinogen. Controlling polymerization can be a problem unless you place embedding molds on top if ice, and cooling the embedding mixture with ice water. The polymerization is exothermic, and actually as blocks polymerize gets uncomfortably hot which may be damaging to enzymes and sensitive antigens although the heat can be dispersed. Samples cannot be any thicker than 2 mm, with 1 mm X 1 mm is recommended. This plastic was first used for liver needle biopsies. Polymerization for larger samples is hard to control as is the infiltration by this plastic hence smaller, thinner samples. Sectioning is commonly done with glass knives although tungsten carbide knives work, and I know of one group using disposable blades with a Leica 2255 model microtome. More powerful microtomes i.e. Leica 2650 or equivalent works best. We had a JB-4 microtome with a special block holder to accommodate the metal ?chucks?/block holders sold by Polysciences. The metal block holders were a better heat sink to disperse heat of polymerization. Sections are generally no thicker than 1 to 3 ?m, and were wonderful when studying single cell protozoa. We never used GMA for more routine tissue sections although it was popular for bone biopsies in clinical labs over the many years. I personally found it labor intensive, and expensive for our projects although the staining results for H&E, PAS-H and some other special stains very nice. Routine stains can be used, including PAS-H, H&E, Massons trichrome with a modified method, and others. IHC will not work well, even with JB-4 Immunobed. GMA, once polymerized, cannot be removed from the section. Immunobed is probably just a looser matrix than JB-4 and some people have success. GMA plastic is less hydrophobic but still will not allow large immunoglobulins to reach antigenic sites. There has been some success with IHC but in general, GMA is not the ideal embedding media for immunostaining. Neil Hand worked with Poly methylmethacrylate for IHC since the plastic can be completely removed from a thin section, followed by stringent HIER using a pressure cooker. PMMA is another world for processing, sectioning and staining. When doing H&E, the staining protocol is different from paraffin section staining. If you do an extensive, time intensive search on Histonet, there are many discussions about GMA staining both for routine and IHC. You can buy kits, JB-4 and Technovits. The JB-4 discolors over the years to a dark tea/brown color making it more difficult to see the tissue while Technovits remains clear. When you cut sections, you work with one section at a time, not a ribbon. I have a huge file on GMA collected from the early 70?s all the way to current years. If you reply to me personally, I can help with references and protocols. In today?s world, if wanting IHC on plastic embedded tissue, I would be using Neil Hands recommendations and Poly methylmethacrylate (PMMA) plastic embedding mainly since this plastic is removable. I also have close contact with Neil if you need to visit with him. He is a good plastics guru and has extensive experience with IHC using PMMA. Using this plastic also requires stringent safety precautions for handling the chemicals. Sorry I leaned towards a negative view of GMA. Take care Gayle Callis HTL/HT/MT(ASCP) GCallis Histology Service, LLC Bozeman Montana USA From gayle.callis at bresnan.net Tue Jul 19 16:39:38 2016 From: gayle.callis at bresnan.net (Gayle Callis) Date: Tue, 19 Jul 2016 16:39:38 -0500 Subject: [Histonet] Attention Daniel Blackburn about plastics embedding Message-ID: <003d01d1e206$0d9f4d40$28dde7c0$@bresnan.net> You wrote: My lab hopes to get into plastic sectioning. We need to be able to process tissue pieces as large and thick as possible, but see that the largest embedding molds for JB4 are only 13x19mm by 5mm deep. We have two questions: (1) Do any of the available media (plastic or resins) allow one to embed and section large pieces (for example eggs with dimensions of 2 cm or larger)? (2) Is a special microtome (such as a retracting microtome) needed? Our reason for considering plastic is that we must section yolk, which splits out of standard paraffin during sectioning. Any advice is appreciated. -- Daniel Blackburn, Trinity College ************************************************************ Glycol methacrylate is not designed for samples as large at 2 cm. However, I have one publication from an old Stain Technology for large chondro-osseous (sp?) sample. I also have a protocol from a lady who used water based processing with GMA since it is water miscible. This was a protocol lipids which are removed by organic solvents. You might be able to develop a protocol with your 2 cm samples. Kits are expensive and I have information on making up GMA in house. Hope you have a good fume hood!! As you can see from another post today, GMA contains sensitizing chemicals, and a carcinogen. It will require double gloving with nitrile gloves, and other personal safety gear. The fume hood is an absolute must have. You will need a powerful microtome, i.e. Leica 2250 or 2265 or equivalent, with very sharp knives, maybe even tungsten carbide. We used glass knives on a JB-4 microtome but I have a colleague who used a 2250 and disposable microtome blades, but you blocks are pretty large. You may want to consider using Peel away molds which come in several sizes. With your size sample, infiltration and polymerization will be tricky. You have to seal air away from the top of molds in order to get the blocks to polymerize. I think people have used plastic wrap over the top of molds to exclude air although our metal blocks fit in the embedding molds snugly and we did an old school method to exclude air. Melted paraffin around outside of molds, a messy but effective air block. Please contact me personally and I will send this this information to you. I think you can do this with a reply to all after reading this message. Take care Gayle Callis HTL, HT, MT(ASCP) GCallis Histology Service LLC Bozeman MT/USA From LRaff at uropartners.com Wed Jul 20 08:10:25 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 20 Jul 2016 13:10:25 +0000 Subject: [Histonet] Lab/health related post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10EB74CE@COLOEXCH01.uropartners.local> Hope all the list serve members are doing well. This is significant news for men; share it with your staff, your family, your friends: http://www.chicagonow.com/downsize-maybe/2016/07/when-it-hurts-to-say-i-told-you-so-advanced-prostate-cancer-cases-on-the-rise/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From blayjorge at gmail.com Wed Jul 20 10:11:17 2016 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Wed, 20 Jul 2016 11:11:17 -0400 Subject: [Histonet] looking for recommendations on a textbook (and/or other materials) suitable for a 100-level college interdisciplinary course in emphasizing science, society, and history Message-ID: Dear Colleagues: I am looking for recommendations on a textbook (and/or other materials) suitable for a 100-level college interdisciplinary course in emphasizing science, society, and history. If you have any constructive recommendations, please kindly send them directly to me: blayjorge at gmail.com Apologies for potential duplicate emails. Gratefully, Jorge From Danielle.Mark at aurora.org Wed Jul 20 11:57:18 2016 From: Danielle.Mark at aurora.org (Mark, Danielle) Date: Wed, 20 Jul 2016 16:57:18 +0000 Subject: [Histonet] Crystals in PAS from Ventana Message-ID: I am wondering if anyone else has been having issues with the PAS stain. The crystals are not rinsing off of the slide using the benchmark special stainer. We are on our 3rd lot number, no change. We have had our tubing changed and our instrument checked to make sure the rinse is appropriate. This problem is causing our lab great frustration. Any feedback would be appreciated. Danielle Mark, HT(ASCP), Technical Specialist ACL Laboratories 8901 W Lincoln Ave. | West Allis, WI 53227 O: 414.328.6235 | F: 414.328.6237 | Danielle.mark at aurora.org [https://mail.ahcmessaging.org/ecp/Customize/14.3.181.6/themes/resources/clear1x1.gif] www.acllaboratories.com From acoscetti at gmail.com Wed Jul 20 15:53:33 2016 From: acoscetti at gmail.com (Amanda Coscetti) Date: Wed, 20 Jul 2016 16:53:33 -0400 Subject: [Histonet] CAP questions Message-ID: <7F91E743-B240-4D59-8547-44328324678C@gmail.com> Anyone have any info on how to handle these 2 CAP questions: COM.30450 New reagent lot confirmation of acceptability COM.40000 method of validation/verification approval Thanks, Amanda Sent from my iPhone From eddessa at emory.edu Wed Jul 20 20:34:36 2016 From: eddessa at emory.edu (Dessasau III, Evan) Date: Thu, 21 Jul 2016 01:34:36 +0000 Subject: [Histonet] I would like help with embedding Kidney needle biopsies Message-ID: Hi all, I am wondering if those of you that work with Kidney Needle biopsies would not mind helping me improve my handling of this tissue type. This work is being done at the pre-clinical level but I still would like to improve my cutting. We uses to process every tissue size in one overnight run. I have moved small tissues to a separate run which I think gives us an improved cutting quality. I think I could even move to a shorter run for the KNB. I still fight with micro-chatter on a 6.5hr. run. I have been reading through the Histonet archives tonight and my eyes are crossed but I did not see anything specific on embedding needle biopsies. I did see some other juicy items I will give a try down the road. I believe fine tuning the processing will not be as hard as learning to get these very small samples in the same plane so that I can get everything in my first section. I hope you will not mind sharing any helpful knowledge. Thank you! E-van ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From jvickroy at SpringfieldClinic.com Thu Jul 21 09:09:05 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Thu, 21 Jul 2016 14:09:05 +0000 Subject: [Histonet] Fading of H&E controls Message-ID: <9B1A1501A800064397369BD8072E6BCA065883B4@E2K10DB.springfieldclinic.com> We are getting ready for our first CAP inspection in the new laboratory. I was checking over the H&E controls that we do daily and made a discovery. Several of the first slides we stained, over a year ago, are now washed out. The eosin staining is probably worse than the hematoxylin however both have faded. The H&E slides are in a file box that sits on the counter. I next checked our surgical slide files and found that there were a very few slides from the same time period that have faded but most were still very good. One of the weird things I have found in the controls is that some slides are as vibrant and brilliant as the day we stained them and some are almost completely faded with little color. I am trouble shooting to figure out why the slides are fading. I am trying to think of the variables between the H&E control slides and the routine surgical slides. The only thing that is different is that the routine surgical slides are filed in a rack and not exposed to the fluorescent light all day. Is this possible? Other thoughts: If it is not the exposure to light than I am wondering if there is a problem with the staining protocol. Another variable is that we use recycled clearing reagents. On the stainer we use Fisher's Citrus Clearing agent and we recycle. We process with Clearite III. I know xylene is the best but do not want to use Xylene because of health issues. I also wonder if there may still be some alcohol in the sections since some frozen sections fade when the clearing is not complete. I do think the fading problem would be more evident in the routine filed slides if the problem was a staining protocol problem, but the recycled clearing agent is still on the table of suspects. In the beginning we did not change the reagents as often as we do now but of course our volumes have increased also. Please share with me your thoughts. Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From akbitting at geisinger.edu Thu Jul 21 09:13:46 2016 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Thu, 21 Jul 2016 14:13:46 +0000 Subject: [Histonet] [External] Crystals in PAS from Ventana In-Reply-To: References: Message-ID: Hi Mark, We still have this issue after two years of complaining to Roche. We've had multiple TAS's come in and we've sent slides to Tucson. They've gone over the instruments, etc. Long story short, their fix was adding something to their package insert that says "Schiff's Reagent is known to contain some needle-like precipitate. At expected levels, this precipitate should not affect assay performance". Good luck. -----Original Message----- From: Mark, Danielle via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, July 20, 2016 12:57 PM To: histonet at lists.utsouthwestern.edu Subject: [External] [Histonet] Crystals in PAS from Ventana I am wondering if anyone else has been having issues with the PAS stain. The crystals are not rinsing off of the slide using the benchmark special stainer. We are on our 3rd lot number, no change. We have had our tubing changed and our instrument checked to make sure the rinse is appropriate. This problem is causing our lab great frustration. Any feedback would be appreciated. Danielle Mark, HT(ASCP), Technical Specialist ACL Laboratories 8901 W Lincoln Ave. | West Allis, WI 53227 O: 414.328.6235 | F: 414.328.6237 | Danielle.mark at aurora.org [https://na01.safelinks.protection.outlook.com/?url=https%3a%2f%2fmail.ahcmessaging.org%2fecp%2fCustomize%2f14.3.181.6%2fthemes%2fresources%2fclear1x1.gif&data=01%7c01%7cakbitting%40geisinger.edu%7c9607113abb9d43ddd71208d3b0bf012b%7c37d46c567c664402a16055c2313b910d%7c0&sdata=g8TNPhBz03xDG6lDMRyYbc%2fAvSSJw4H4EP97Q3kkQHw%3d] https://na01.safelinks.protection.outlook.com/?url=www.acllaboratories.com&data=01%7c01%7cakbitting%40geisinger.edu%7c9607113abb9d43ddd71208d3b0bf012b%7c37d46c567c664402a16055c2313b910d%7c0&sdata=LvrQWkxweaSX2uY5hkm%2bmTHafS68Ig%2b1S%2faN1dgRtV8%3d _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c9607113abb9d43ddd71208d3b0bf012b%7c37d46c567c664402a16055c2313b910d%7c0&sdata=sirNu%2f8R%2bWnPicFogFkyOgINlvWbfQDfEXCELv7wodc%3d IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From liz at premierlab.com Thu Jul 21 09:57:41 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Thu, 21 Jul 2016 08:57:41 -0600 Subject: [Histonet] Fading of H&E controls -long response with portions of SOP Message-ID: <14E2C6176416974295479C64A11CB9AE02BED535B432@SBS2K8.premierlab.local> James We use recycled xylene in our H&E stainer and have never experienced any fading of the eosin. When you recycle your clearing agent do you check for purity and contamination, you have to also take into consideration that xylene substitutes may behave differently than xylene. We have an SOP that governs how we recycle, we keep tract of runs and assign lot numbers to recycled products, and we also record what lot numbers were used when we change the stainer or tissue processer, so if we do have problems (we normally do not) we could trace back to the lot of recycled reagent. This is how we test for xylene purity - see below. For our recycled alcohol we do merge multiple runs since we will use this to make up solutions but we also have a defined procedure for that and it includes determining the percentage of alcohol via hydrometer and adjusting for temperature prior to making up a reagent with it. Excess alcohol in your xylene can also cause reagents to fade, especially ones that are susceptible to fading in alcohol such as some of the red chromogens for IHC. This could be due to the process of recycling or your overall QC and how often you change your stainer. 8.6 Testing the Purity of Recycled Xylene or Propar 8.6.1 Pour 85ml of recycled xylene or propar into a clean, dry 100ml graduated cylinder, then add 15ml deionized water. 8.6.2 Seal the top of the graduated cylinder and invert the mixture a couple of times. 8.6.3 Allow the mixture to settle. Make sure all the water settles to the bottom of the graduated cylinder and does not cling to the sides above the xylene/ or propar/water separation point (approximately the 15ml level of the graduated cylinder). 8.6.4 Record the water/solvent separation point as indicated by the bottom of the meniscus on Form 1: Solvent Recycler Log - EQ-0014.1. 8.6.5 Use the following equations to calculate the percent impurities and percent purity of the recycled xylene: (measured separation point) - 14.9 = (% impurities) 100 - (% impurities) = (% pure xylene) 8.6.6 Enter the percent pure xylene on Form 1: Solvent Recycler Log - EQ-0014.1. 8.7 Assigning Control Numbers for Xylene and Propar 8.7.1 Each recycled lot will be kept in a separate carboy, and assigned a unique control number. 8.7.2 Control numbers for xylene have the format: two digit year (YY)-RXYL-lot #, and control numbers for propar have the format: two digit year (YY)-RPRO-lot #. 8.7.3 Write the assigned control number and the expiration date (one year from recycling) on Form 1: Solvent Recycler Log - EQ-0014.1. 8.7.4 Fill out a reagent control label (see Appendix 1) to label the carboy. 8.7.5 Recycled alcohol will not be assigned control numbers since multiple lots are combined and it is only used in making other reagents. Instead, control numbers are assigned to the solutions made from recycled alcohol (50%, 70%, 80%, 95% or other). 8.8 Testing Recycled Alcohol for Xylene Contamination 8.8.1 Every batch of recycled alcohol should be tested for xylene contamination. 8.8.2 Mix a small volume of recycled alcohol with an equal volume of deionized water. 8.8.3 If the recycled alcohol is contaminated with xylene, the mixture will become cloudy or milky. 8.8.4 If contamination is discovered, dispose of the recycled product, clean all containers thoroughly (see 8.1.1), and flush the recycler (see 7.3.7) before proceeding with the next batch. 8.9 Measuring the Percent Alcohol of Recycled Alcohol Using a Hydrometer 8.9.1 A hydrometer measures specific gravity and is used to determine the percent alcohol of recycled alcohol produced by the CBG Biotech Solvent Recycler System. 8.9.2 When using recycled alcohol to make alcohol solutions (50%, 70%, 80%, 95% or other) it is necessary to determine the percent alcohol of the current stock of recycled alcohol. 8.9.3 Since water and alcohol have different densities, and therefore different specific gravities, the specific gravity of a mixture of these two liquids indicates the percent alcohol of the solution. 8.9.4 The temperature of a liquid affects its density, and therefore its specific gravity. Because of this, hydrometer reading must be adjusted for temperature. 8.9.5 Since the alcohol collected from the recycler is slightly warm, it is best to let it stand at room temperature overnight before using it to make alcohol solutions. 8.9.6 Fill a transparent 500ml graduated cylinder with a sample of the recycled alcohol and gently place the hydrometer into the solution, such that it is floating with the weighted end down. Allow the hydrometer to stop moving, then record the reading (Tralles, not Proof) at the bottom of the meniscus on the reagent control form. 8.9.7 Measure the temperature of the alcohol in the graduated cylinder and record it on the reagent control form. 8.9.8 Like most standard hydrometers, the hydrometer used in Premier Laboratory is calibrated at 60?F. Form 2: Temperature Correction Chart for Hydrometer Readings - EQ-0014.2 shows the correction factors for hydrometer readings over a range of temperatures. 8.9.9 Calculate the actual percent alcohol from the measured percent alcohol using the following equation: (measured % alcohol) - (measured % alcohol x correction factor) = (corrected % alcohol) 8.9.10 Record the corrected percent alcohol on the reagent control form, and use it to calculate the correct volumes of recycled alcohol and deionized water or reagent alcohol for the desired solution. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Vickroy, James via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 21, 2016 8:09 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fading of H&E controls We are getting ready for our first CAP inspection in the new laboratory. I was checking over the H&E controls that we do daily and made a discovery. Several of the first slides we stained, over a year ago, are now washed out. The eosin staining is probably worse than the hematoxylin however both have faded. The H&E slides are in a file box that sits on the counter. I next checked our surgical slide files and found that there were a very few slides from the same time period that have faded but most were still very good. One of the weird things I have found in the controls is that some slides are as vibrant and brilliant as the day we stained them and some are almost completely faded with little color. I am trouble shooting to figure out why the slides are fading. I am trying to think of the variables between the H&E control slides and the routine surgical slides. The only thing that is different is that the routine surgical slides are filed in a rack and not exposed to the fluorescent light all day. Is this possible? Other thoughts: If it is not the exposure to light than I am wondering if there is a problem with the staining protocol. Another variable is that we use recycled clearing reagents. On the stainer we use Fisher's Citrus Clearing agent and we recycle. We process with Clearite III. I know xylene is the best but do not want to use Xylene because of health issues. I also wonder if there may still be some alcohol in the sections since some frozen sections fade when the clearing is not complete. I do think the fading problem would be more evident in the routine filed slides if the problem was a staining protocol problem, but the recycled clearing agent is still on the table of suspects. In the beginning we did not change the reagents as often as we do now but of course our volumes have increased also. Please share with me your thoughts. Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abuchiane at bmhvt.org Thu Jul 21 10:32:29 2016 From: abuchiane at bmhvt.org (Anita Buchiane) Date: Thu, 21 Jul 2016 15:32:29 +0000 Subject: [Histonet] Reticulin Stain Message-ID: <4034E71604330C4B8E10D1538DFB2455ECC95EE9@BMHEXCH02.bmhvt.org> Does anyone out there still do the retic by hand? If so, can you share which procedure you use? Thanks _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ From aaditza1 at gmail.com Thu Jul 21 10:48:24 2016 From: aaditza1 at gmail.com (Aditza) Date: Thu, 21 Jul 2016 10:48:24 -0500 Subject: [Histonet] Best semi-automated/automated TMA instrument to buy? Message-ID: <580E61A4-C587-4813-91E2-6FC72F8323E1@gmail.com> Dear all amazing histology people, Our lab is looking to buy a New great semi-automated/automated TMA instrument. I worked before on manual Beecher and semi automated Veridiam. They are just ok. Do you have any suggestions? I think Beecher has an automatic one? Thank you and have a wonderful day! Sincerely, Adriana Rosca HTL (ASCP) MS "...what we say and how we say it, is still the foundation of behavior change." From emartinez2 at echd.org Thu Jul 21 10:53:39 2016 From: emartinez2 at echd.org (Estela Martinez) Date: Thu, 21 Jul 2016 15:53:39 +0000 Subject: [Histonet] MCH IHC Message-ID: Hello My Histo Friends!! Does anybody have an extra MCK RTU Antibody-- Catalog No. PA0909-- Clone AE1 and AE3, for the Leica Bond Max machine. that we can borrow until we get ours in. Apparently it's been back ordered til Aug 1 and we are out. Please let me know if someone can help us out. Thank you in advance!! Estela Martinez Histology Supervisor Medical Center Hospital Odessa, TX 79761 432-640-2348 emartinez2 at echd.org CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party without permission of original user and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From rjbuesa at yahoo.com Thu Jul 21 11:09:11 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 21 Jul 2016 16:09:11 +0000 (UTC) Subject: [Histonet] Reticulin Stain In-Reply-To: <4034E71604330C4B8E10D1538DFB2455ECC95EE9@BMHEXCH02.bmhvt.org> References: <4034E71604330C4B8E10D1538DFB2455ECC95EE9@BMHEXCH02.bmhvt.org> Message-ID: <428463406.982462.1469117351352.JavaMail.yahoo@mail.yahoo.com> Gomori's Ren? On Thursday, July 21, 2016 12:04 PM, Anita Buchiane via Histonet wrote: Does anyone out there still do the retic by hand?? If so, can you share which procedure you use?? Thanks _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JRobinson at pathology-associates.com Thu Jul 21 11:47:01 2016 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Thu, 21 Jul 2016 16:47:01 +0000 Subject: [Histonet] MCH IHC In-Reply-To: References: Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C90AEEAA9@PAEXCH1.PathologyAssociates.local> Hi Estela- I had the same problem with the Bond multicytokeratin. I currently have 3 vials of the "old" one on backorder. I had to validate the new "reformulation" as I have now run out of the old one (PA0909). Have your rep send you the new one (PA0012). My rep sent me one for free. It seems to be a bit stronger than the old one but I ended up using the same protocol (ER2 for 20 minutes). CAUTION!!! If you do not have the newest database downloaded onto your instrument you will need to get that done. If you have the old version 4.0 software like I do it is a different database. Call Leica tech support for help as you may CRASH YOUR DATABASE if you download it incorrectly. I needed Database 67. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Estela Martinez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 21, 2016 8:54 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] MCH IHC Hello My Histo Friends!! Does anybody have an extra MCK RTU Antibody-- Catalog No. PA0909-- Clone AE1 and AE3, for the Leica Bond Max machine. that we can borrow until we get ours in. Apparently it's been back ordered til Aug 1 and we are out. Please let me know if someone can help us out. Thank you in advance!! Estela Martinez Histology Supervisor Medical Center Hospital Odessa, TX 79761 432-640-2348 emartinez2 at echd.org CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party without permission of original user and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From fatimaformadi at gmail.com Thu Jul 21 13:17:50 2016 From: fatimaformadi at gmail.com (Fatima Formadi) Date: Thu, 21 Jul 2016 12:17:50 -0600 Subject: [Histonet] Unscribe Message-ID: Hi, I wish to unscribe from this mailing list immediately please. Thank you Regards, Fatima From lmarie08 at uga.edu Thu Jul 21 13:46:53 2016 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Thu, 21 Jul 2016 18:46:53 +0000 Subject: [Histonet] block scrapers Message-ID: Hi all, My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me? Thanks!! L From jaylundgren at gmail.com Thu Jul 21 14:04:06 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 21 Jul 2016 14:04:06 -0500 Subject: [Histonet] block scrapers In-Reply-To: References: Message-ID: Buy cheap paring knives at the dollar store and blunt the edges? On Thu, Jul 21, 2016 at 1:46 PM, Lauren Sweeney via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all, > > My lab is in need of some tools to scrap the paraffin off the edges of the > blocks after embedding. Does anyone have any recommendations for me? > > > Thanks!! > L > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bcooper at chla.usc.edu Thu Jul 21 14:08:02 2016 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Thu, 21 Jul 2016 19:08:02 +0000 Subject: [Histonet] block scrapers In-Reply-To: References: Message-ID: I bought everyone really old pocket knives from flea markets--basically the same thing as what Jay said. For the most part, these old knives were already really dull and didn't require any assistance to dull them further. Good luck. Brian -----Original Message----- From: Jay Lundgren via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 21, 2016 12:04 PM To: Lauren Sweeney Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] block scrapers Buy cheap paring knives at the dollar store and blunt the edges? On Thu, Jul 21, 2016 at 1:46 PM, Lauren Sweeney via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all, > > My lab is in need of some tools to scrap the paraffin off the edges of > the blocks after embedding. Does anyone have any recommendations for me? > > > Thanks!! > L > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From liz at premierlab.com Thu Jul 21 14:09:13 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Thu, 21 Jul 2016 13:09:13 -0600 Subject: [Histonet] block scrapers In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02BED535B447@SBS2K8.premierlab.local> Lauren I have always used a very dull steak knife (sharp ones are too sharp and will cut the cassette) mine is probably over 20 years old (now I'm dating myself). I'm old school so I still use that but the rest of the techs will use the para trimmer. The para trimmer is more ergonomically friendly less repetitive than a knife - here is the link to the website https://www.thermofisher.com/order/catalog/product/B3120205 Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Lauren Sweeney via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 21, 2016 12:47 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] block scrapers Hi all, My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me? Thanks!! L _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aj.taylor at blueyonder.co.uk Thu Jul 21 14:30:31 2016 From: aj.taylor at blueyonder.co.uk (taylor alan) Date: Thu, 21 Jul 2016 20:30:31 +0100 (BST) Subject: [Histonet] Reticulin Stain In-Reply-To: <428463406.982462.1469117351352.JavaMail.yahoo@mail.yahoo.com> References: <4034E71604330C4B8E10D1538DFB2455ECC95EE9@BMHEXCH02.bmhvt.org> <428463406.982462.1469117351352.JavaMail.yahoo@mail.yahoo.com> Message-ID: <846648922.1768040.1469129431802.JavaMail.open-xchange@oxbe1.tb.ukmail.iss.as9143.net> Hi All Here in our lab we routinely hand stain reticulin sections. Our preferred choice is the Gordon and Sweet method. The technique is easy to perform, diffing out can be easily controlled via the staining microscope and a variety of counterstains can be used in addition to the usual Neutral Red. We still prefer to prepare our own in-house solutions and reagents rather than purchase expensive commercial products. Old fashioned I know! But this is the common way still in many small, independent UK labs. Histonet is such a valuable resource. I look forward to reading daily submissions and comments. Alan Taylor Microtechnical Services Exeter. Devon. UK > > On 21 July 2016 at 17:09 Rene J Buesa via Histonet > wrote: > > > Gomori's Ren? > > On Thursday, July 21, 2016 12:04 PM, Anita Buchiane via Histonet > wrote: > > > Does anyone out there still do the retic by hand? If so, can you share > which procedure you use? Thanks > > > > > _______________________________________________________________ > > The information contained in, or attached to, this e-mail, may contain > confidential information and is intended solely for the use of the individual > or entity to whom it is addressed and may be subject to legal privilege. If > you have received this e-mail in error you should notify the sender > immediately by reply e-mail, delete the message from your system and notify > your system manager. Please do not copy it for any purpose, or disclose its > contents to any other person. The views or opinions presented in this e-mail > are solely those of the author and do not necessarily represent those of the > company. The recipient should check this e-mail and any attachments for the > presence of viruses. The company accepts no liability for any damage caused, > directly or indirectly, by any virus transmitted in this email. > _______________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jford at cytomx.com Thu Jul 21 14:31:17 2016 From: jford at cytomx.com (Judi Ford) Date: Thu, 21 Jul 2016 19:31:17 +0000 Subject: [Histonet] H&E staining on tape Message-ID: Hi everyone, I have a colleague that has been asked to do H&E staining on a frozen specimen collected on tape. The tape has been attached to the slide using nail polish. Can't use the CryoJane system due to the light flash. Her question is "will the nail polish disintegrate during the immersion in alcohol and xylene?". Another question is will the stains attach to the tape and obscure the tissue? If so, what to do with about that. She is also worried that the tape will fall off into the staining dish and get lost. I'm thinking maybe using a 6 well disposable tissue culture plate for the staining of the tape then reattach it to the slide and coverslip. Any suggestions? Thanks so much, Judi From craigak12 at gmail.com Thu Jul 21 14:32:48 2016 From: craigak12 at gmail.com (J B) Date: Thu, 21 Jul 2016 19:32:48 +0000 Subject: [Histonet] block scrapers In-Reply-To: References: Message-ID: Yes, search paratrimmer you will love it. It melts excess paraffin off blocks. JB On Thu, Jul 21, 2016, 11:52 AM Lauren Sweeney via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all, > > My lab is in need of some tools to scrap the paraffin off the edges of the > blocks after embedding. Does anyone have any recommendations for me? > > > Thanks!! > L > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JMacDonald at mtsac.edu Thu Jul 21 14:45:55 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Thu, 21 Jul 2016 12:45:55 -0700 Subject: [Histonet] block scrapers In-Reply-To: References: , Message-ID: From JMacDonald at mtsac.edu Thu Jul 21 14:51:41 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Thu, 21 Jul 2016 12:51:41 -0700 Subject: [Histonet] Reticulin Stain In-Reply-To: <4034E71604330C4B8E10D1538DFB2455ECC95EE9@BMHEXCH02.bmhvt.org> References: <4034E71604330C4B8E10D1538DFB2455ECC95EE9@BMHEXCH02.bmhvt.org> Message-ID: From rjbuesa at yahoo.com Thu Jul 21 15:52:26 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 21 Jul 2016 20:52:26 +0000 (UTC) Subject: [Histonet] block scrapers In-Reply-To: References: Message-ID: <1022675503.2796010.1469134346522.JavaMail.yahoo@mail.yahoo.com> Use a regular one blade pocket knife (as?I used to do).Ren? On Thursday, July 21, 2016 2:49 PM, Lauren Sweeney via Histonet wrote: Hi all, My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me? Thanks!! L _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks at gmail.com Fri Jul 22 03:52:37 2016 From: amosbrooks at gmail.com (Amos Brooks) Date: Fri, 22 Jul 2016 04:52:37 -0400 Subject: [Histonet] Crystals in PAS In-Reply-To: References: Message-ID: Hi, If you are having trouble with the instrument and the manufacturer isn't able to fix it, just hand stain them. PAS is really not a complicated stain. Please don't just accept underperforming equipment when we are all capable of so much more. Amos From rjr6 at psu.edu Fri Jul 22 09:14:48 2016 From: rjr6 at psu.edu (Roberta Horner) Date: Fri, 22 Jul 2016 14:14:48 +0000 Subject: [Histonet] block scrapers In-Reply-To: <14E2C6176416974295479C64A11CB9AE02BED535B447@SBS2K8.premierlab.local> References: , <14E2C6176416974295479C64A11CB9AE02BED535B447@SBS2K8.premierlab.local> Message-ID: <922f26cac06547eeb147719d6354acd5@PSU.EDU> I use a single edge razor blade that I've opened boxes with and is dulled to just the right sharpness. And I wish people would quit throwing it away Roberta Horner Animal Diagnostic Lab Penn State From criley at dpspa.com Fri Jul 22 09:42:50 2016 From: criley at dpspa.com (Charles Riley) Date: Fri, 22 Jul 2016 10:42:50 -0400 Subject: [Histonet] Air Bubbles Message-ID: Has anyone encountered the following problem. Tissue cut and is processed well. There is no distortion at a cellular level. However anywhere from 30 to 2 days later there are air bubbles under the coverslip. I know for certain there aren't even the slightest bit of air bubbles when coverslipped. I have a feeling it could still be a processing issue as I ran a few tests like coverslipping blank slides and tissue sections from a year ago when there was no issues. If anyone has any suggestions as to what the problem could be and potential solutions please get back to me as soon as possible as this is becoming a major issue for my lab. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From Timothy.Morken at ucsf.edu Fri Jul 22 10:29:58 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 22 Jul 2016 15:29:58 +0000 Subject: [Histonet] block scrapers In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF6FD6B2BE@ex07.net.ucsf.edu> Sakura has purpose-made scrapes. Otherwise you can use plastic scrapers from a hardware store. However, we moved away from scrapers and now use a heating block trimmer made for the purpose. Several vendors offer these. http://www.newcomersupply.com/product/paraffin-wax-trimmer We do all block trimming at a central location so it does not need to be done at the microtome. It is very quick and does not damage the print on the block. That is especially important for barcoded blocks. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Lauren Sweeney via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 21, 2016 11:47 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] block scrapers Hi all, My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me? Thanks!! L _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jvickroy at SpringfieldClinic.com Fri Jul 22 13:32:56 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Fri, 22 Jul 2016 18:32:56 +0000 Subject: [Histonet] Mohs lab - CLIA or CAP inspections Message-ID: <9B1A1501A800064397369BD8072E6BCA065897F9@E2K10DB.springfieldclinic.com> We have a Mohs lab that is in the process of moving and will end up with a new CLIA number. The Mohs physician is more familiar with CLIA certification and would like for us to choose CLIA inspections instead of CAP inspections. I am very familiar with CAP inspections although not very familiar with the Mohs lab inspections done in the past. Can someone comment on the difference between CLIA and CAP inspections of a Mohs lab? The medical director of the current Histology lab believes that having the new Mohs lab under CLIA instead of CAP will be more difficult than we have now. Can anyone share any experiences or thoughts? Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From rennie1108 at yahoo.com Fri Jul 22 14:49:22 2016 From: rennie1108 at yahoo.com (Adrienne Anderson) Date: Fri, 22 Jul 2016 19:49:22 +0000 (UTC) Subject: [Histonet] Fixation for FISH References: <1889116520.3116377.1469216962421.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1889116520.3116377.1469216962421.JavaMail.yahoo@mail.yahoo.com> Hello Histo-land, I'm going to start doing FISH in our lab, but I've never done it before. I read that the PFA fixative solution should be made fresh; however, I have some PFA that is being stored in the -80 freezer (for less than a year). Does anyone know if this would be ok to use? And actually, if it is ok, I thought about making a batch of 3% PFA and aliquoting it into smaller containers to freeze and to use at a later time. The only reason I thought about doing that is that I've heard to make it can take awhile. But this is all new to me, so any and all help would be much appreciated. I might as well add this as well: specifically, I'll be trying to optimize a protocol for Texas red-2-O-methyl-CAG probe with IF for the MBNL1 protein. I've read that combining these 2 methods can be tricky....and I've never done either of them! Is there anyone out there that can give me some advice? Thanks!Adrienne From SteveM at mcclainlab.com Fri Jul 22 16:49:52 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Fri, 22 Jul 2016 21:49:52 +0000 Subject: [Histonet] Histonet Digest, Vol 152, Issue 18 In-Reply-To: References: Message-ID: <81D1263B-C39D-4D4D-B01C-8763298FC014@mcclainlab.com> PAS crystals. The problem may not be the stain at all. occurring due to conditions to leaching of biochemical substances, in the tissue. Dirty processors, or use of KOH or acids applied to the block just before sectioning. Those may not be washed out. These crystals are especially common in thin 1-2mm fungal toe nails. I can tell you how to get at it, but it's a long story. And a bit of science to move you toward a solution. Kindly send me a private email. The fungus stuff is too weird to share in mixed company. Your pathologist will resist or not believe it. Heck if I didn't have calibrated photos, I wouldn't believe it either. The toenails are so weird, I am working to improve w 3D gross images. Steve A. McClain, MD From Hlukey at msn.com Fri Jul 22 17:32:37 2016 From: Hlukey at msn.com (Hugh Luk) Date: Fri, 22 Jul 2016 22:32:37 +0000 Subject: [Histonet] semi-automated/automated TMA instrument to buy? In-Reply-To: References: Message-ID: ________________________________ Hi Adriana, We had a Beecher but it acted stupid, so we got a Pathology Devices semi-automated arrayer. We typically build ~ 100 blocks per year, under a variety of study banners, so it's ideal for us. The machine is mostly manual, but is durable and Ron (owner) should be commended on how helpful he is. Our clientele have expressed the excellent quality of our finished products. I like the fact that I can do 4 identical TMA recipients (if needed) and I do not have to flip the block over. Fully automated unit? I know people running the Beecher-fully automated unit, and they run their units overnight, 7 days/week. If you google TMA arrayers, you should get a few units to look at (ie. http://www.ihcworld.com/products/Tissue-Microarray-Instrument.htm), but I can't personally vouch for any fully automated unit. Hugh Hawaii Tissue Microarrayers and Kits - IHC WORLD www.ihcworld.com EZ-TMA Manual Tissue Microarray Kits. IHC Wo rld is now introducing a new series of EZ-TMA TM Tissue Microarray Kits that can be applied to any laboratories for ... ------------------------------ Date: Thu, 21 Jul 2016 10:48:24 -0500 From: Aditza To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Best semi-automated/automated TMA instrument to buy? Message-ID: <580E61A4-C587-4813-91E2-6FC72F8323E1 at gmail.com> Content-Type: text/plain; charset=us-ascii Dear all amazing histology people, Our lab is looking to buy a New great semi-automated/automated TMA instrument. I worked before on manual Beecher and semi automated Veridiam. They are just ok. Do you have any suggestions? I think Beecher has an automatic one? Thank you and have a wonderful day! Sincerely, Adriana Rosca HTL (ASCP) MS "...what we say and how we say it, is still the foundation of behavior change." ------------------------------ From chardy at csu.edu.au Sun Jul 24 19:40:24 2016 From: chardy at csu.edu.au (Hardy, Cate) Date: Mon, 25 Jul 2016 00:40:24 +0000 Subject: [Histonet] block Scrapers In-Reply-To: References: Message-ID: We just use a blunt butcher knife. It works great. Low tech, low cost! Cate Hardy Senior Technical Officer (Mon-Tue) | Veterinary Enterprises, Faculty of Science Charles Sturt University Boorooma Street Locked Bag 588 Wagga Wagga, NSW 2678 Australia Tel: 69334000 Email: vdl at csu.edu.au Email: chardy at csu.edu.au www.csu.edu.au -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Saturday, 23 July 2016 3:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 152, Issue 20 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Unscribe (Fatima Formadi) 2. block scrapers (Lauren Sweeney) 3. Re: block scrapers (Jay Lundgren) 4. Re: block scrapers (Cooper, Brian) 5. Re: block scrapers (Elizabeth Chlipala) 6. Re: Reticulin Stain (taylor alan) 7. H&E staining on tape (Judi Ford) 8. Re: block scrapers (J B) 9. Re: block scrapers (Jennifer MacDonald) 10. Re: Reticulin Stain (Jennifer MacDonald) 11. Re: block scrapers (Rene J Buesa) 12. Crystals in PAS (Amos Brooks) 13. Re: block scrapers (Roberta Horner) 14. Air Bubbles (Charles Riley) 15. Re: block scrapers (Morken, Timothy) ---------------------------------------------------------------------- Message: 1 Date: Thu, 21 Jul 2016 12:17:50 -0600 From: Fatima Formadi To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Unscribe Message-ID: Content-Type: text/plain; charset=UTF-8 Hi, I wish to unscribe from this mailing list immediately please. Thank you Regards, Fatima ------------------------------ Message: 2 Date: Thu, 21 Jul 2016 18:46:53 +0000 From: Lauren Sweeney To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] block scrapers Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me? Thanks!! L ------------------------------ Message: 3 Date: Thu, 21 Jul 2016 14:04:06 -0500 From: Jay Lundgren To: Lauren Sweeney Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] block scrapers Message-ID: Content-Type: text/plain; charset=UTF-8 Buy cheap paring knives at the dollar store and blunt the edges? On Thu, Jul 21, 2016 at 1:46 PM, Lauren Sweeney via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all, > > My lab is in need of some tools to scrap the paraffin off the edges of > the blocks after embedding. Does anyone have any recommendations for me? > > > Thanks!! > L > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 4 Date: Thu, 21 Jul 2016 19:08:02 +0000 From: "Cooper, Brian" To: "Jay Lundgren" , "Lauren Sweeney" Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] block scrapers Message-ID: Content-Type: text/plain; charset=us-ascii I bought everyone really old pocket knives from flea markets--basically the same thing as what Jay said. For the most part, these old knives were already really dull and didn't require any assistance to dull them further. Good luck. Brian -----Original Message----- From: Jay Lundgren via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 21, 2016 12:04 PM To: Lauren Sweeney Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] block scrapers Buy cheap paring knives at the dollar store and blunt the edges? On Thu, Jul 21, 2016 at 1:46 PM, Lauren Sweeney via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all, > > My lab is in need of some tools to scrap the paraffin off the edges of > the blocks after embedding. Does anyone have any recommendations for me? > > > Thanks!! > L > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- ------------------------------ Message: 5 Date: Thu, 21 Jul 2016 13:09:13 -0600 From: Elizabeth Chlipala To: Lauren Sweeney , "'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)" Subject: Re: [Histonet] block scrapers Message-ID: <14E2C6176416974295479C64A11CB9AE02BED535B447 at SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Lauren I have always used a very dull steak knife (sharp ones are too sharp and will cut the cassette) mine is probably over 20 years old (now I'm dating myself). I'm old school so I still use that but the rest of the techs will use the para trimmer. The para trimmer is more ergonomically friendly less repetitive than a knife - here is the link to the website https://www.thermofisher.com/order/catalog/product/B3120205 Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Lauren Sweeney via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 21, 2016 12:47 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] block scrapers Hi all, My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me? Thanks!! L _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 21 Jul 2016 20:30:31 +0100 (BST) From: taylor alan To: Rene J Buesa , Anita Buchiane , Rene J Buesa via Histonet Subject: Re: [Histonet] Reticulin Stain Message-ID: <846648922.1768040.1469129431802.JavaMail.open-xchange at oxbe1.tb.ukmail.iss.as9143.net> Content-Type: text/plain; charset=UTF-8 Hi All Here in our lab we routinely hand stain reticulin sections. Our preferred choice is the Gordon and Sweet method. The technique is easy to perform, diffing out can be easily controlled via the staining microscope and a variety of counterstains can be used in addition to the usual Neutral Red. We still prefer to prepare our own in-house solutions and reagents rather than purchase expensive commercial products. Old fashioned I know! But this is the common way still in many small, independent UK labs. Histonet is such a valuable resource. I look forward to reading daily submissions and comments. Alan Taylor Microtechnical Services Exeter. Devon. UK > > On 21 July 2016 at 17:09 Rene J Buesa via Histonet > wrote: > > > Gomori's Ren? > > On Thursday, July 21, 2016 12:04 PM, Anita Buchiane via Histonet > wrote: > > > Does anyone out there still do the retic by hand? If so, can you > share which procedure you use? Thanks > > > > > _______________________________________________________________ > > The information contained in, or attached to, this e-mail, may > contain confidential information and is intended solely for the use of > the individual or entity to whom it is addressed and may be subject to > legal privilege. If you have received this e-mail in error you should > notify the sender immediately by reply e-mail, delete the message from > your system and notify your system manager. Please do not copy it for > any purpose, or disclose its contents to any other person. The views > or opinions presented in this e-mail are solely those of the author > and do not necessarily represent those of the company. The recipient > should check this e-mail and any attachments for the presence of > viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. > _______________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Thu, 21 Jul 2016 19:31:17 +0000 From: Judi Ford To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] H&E staining on tape Message-ID: Content-Type: text/plain; charset="us-ascii" Hi everyone, I have a colleague that has been asked to do H&E staining on a frozen specimen collected on tape. The tape has been attached to the slide using nail polish. Can't use the CryoJane system due to the light flash. Her question is "will the nail polish disintegrate during the immersion in alcohol and xylene?". Another question is will the stains attach to the tape and obscure the tissue? If so, what to do with about that. She is also worried that the tape will fall off into the staining dish and get lost. I'm thinking maybe using a 6 well disposable tissue culture plate for the staining of the tape then reattach it to the slide and coverslip. Any suggestions? Thanks so much, Judi ------------------------------ Message: 8 Date: Thu, 21 Jul 2016 19:32:48 +0000 From: J B To: Lauren Sweeney , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] block scrapers Message-ID: Content-Type: text/plain; charset=UTF-8 Yes, search paratrimmer you will love it. It melts excess paraffin off blocks. JB On Thu, Jul 21, 2016, 11:52 AM Lauren Sweeney via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all, > > My lab is in need of some tools to scrap the paraffin off the edges of > the blocks after embedding. Does anyone have any recommendations for me? > > > Thanks!! > L > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Thu, 21 Jul 2016 12:45:55 -0700 From: Jennifer MacDonald To: J B Cc: "histonet at lists.utsouthwestern.edu" ,Lauren Sweeney Subject: Re: [Histonet] block scrapers Message-ID: Content-Type: text/plain; charset="UTF-8" ------------------------------ Message: 10 Date: Thu, 21 Jul 2016 12:51:41 -0700 From: Jennifer MacDonald To: Anita Buchiane Cc: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Reticulin Stain Message-ID: Content-Type: text/plain; charset="UTF-8" ------------------------------ Message: 11 Date: Thu, 21 Jul 2016 20:52:26 +0000 (UTC) From: Rene J Buesa To: Lauren Sweeney , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] block scrapers Message-ID: <1022675503.2796010.1469134346522.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Use a regular one blade pocket knife (as?I used to do).Ren? On Thursday, July 21, 2016 2:49 PM, Lauren Sweeney via Histonet wrote: Hi all, My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me? Thanks!! L _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Fri, 22 Jul 2016 04:52:37 -0400 From: Amos Brooks To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Crystals in PAS Message-ID: Content-Type: text/plain; charset=UTF-8 Hi, If you are having trouble with the instrument and the manufacturer isn't able to fix it, just hand stain them. PAS is really not a complicated stain. Please don't just accept underperforming equipment when we are all capable of so much more. Amos ------------------------------ Message: 13 Date: Fri, 22 Jul 2016 14:14:48 +0000 From: Roberta Horner To: "Histonet (histonet at lists.utsouthwestern.edu)" Subject: Re: [Histonet] block scrapers Message-ID: <922f26cac06547eeb147719d6354acd5 at PSU.EDU> Content-Type: text/plain; charset="iso-8859-1" I use a single edge razor blade that I've opened boxes with and is dulled to just the right sharpness. And I wish people would quit throwing it away Roberta Horner Animal Diagnostic Lab Penn State ------------------------------ Message: 14 Date: Fri, 22 Jul 2016 10:42:50 -0400 From: Charles Riley To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Air Bubbles Message-ID: Content-Type: text/plain; charset=UTF-8 Has anyone encountered the following problem. Tissue cut and is processed well. There is no distortion at a cellular level. However anywhere from 30 to 2 days later there are air bubbles under the coverslip. I know for certain there aren't even the slightest bit of air bubbles when coverslipped. I have a feeling it could still be a processing issue as I ran a few tests like coverslipping blank slides and tissue sections from a year ago when there was no issues. If anyone has any suggestions as to what the problem could be and potential solutions please get back to me as soon as possible as this is becoming a major issue for my lab. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Message: 15 Date: Fri, 22 Jul 2016 15:29:58 +0000 From: "Morken, Timothy" To: Lauren Sweeney Cc: Histonet Subject: Re: [Histonet] block scrapers Message-ID: <761E2B5697F795489C8710BCC72141FF6FD6B2BE at ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Sakura has purpose-made scrapes. Otherwise you can use plastic scrapers from a hardware store. However, we moved away from scrapers and now use a heating block trimmer made for the purpose. Several vendors offer these. http://www.newcomersupply.com/product/paraffin-wax-trimmer We do all block trimming at a central location so it does not need to be done at the microtome. It is very quick and does not damage the print on the block. That is especially important for barcoded blocks. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Lauren Sweeney via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 21, 2016 11:47 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] block scrapers Hi all, My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me? Thanks!! L _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 152, Issue 20 ***************************************** Charles Sturt University | ALBURY-WODONGA | BATHURST | CANBERRA | DUBBO | GOULBURN | MELBOURNE | ORANGE | PORT MACQUARIE | SYDNEY | WAGGA WAGGA | LEGAL NOTICE This email (and any attachment) is confidential and is intended for the use of the addressee(s) only. If you are not the intended recipient of this email, you must not copy, distribute, take any action in reliance on it or disclose it to anyone. Any confidentiality is not waived or lost by reason of mistaken delivery. Email should be checked for viruses and defects before opening. Charles Sturt University (CSU) does not accept liability for viruses or any consequence which arise as a result of this email transmission. Email communications with CSU may be subject to automated email filtering, which could result in the delay or deletion of a legitimate email before it is read at CSU. The views expressed in this email are not necessarily those of CSU. Charles Sturt University in Australia http://www.csu.edu.au The Grange Chancellery, Panorama Avenue, Bathurst NSW Australia 2795 (ABN: 83 878 708 551; CRICOS Provider Numbers: 00005F (NSW), 01947G (VIC), 02960B (ACT)). TEQSA Provider Number: PV12018 Consider the environment before printing this email. From LRaff at uropartners.com Mon Jul 25 08:10:04 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 25 Jul 2016 13:10:04 +0000 Subject: [Histonet] Anatomic path related blog post. Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10ED0916@COLOEXCH01.uropartners.local> Hello all-probably last lab blog post for the next few weeks. http://www.chicagonow.com/downsize-maybe/2016/07/but-doctor-how-do-you-know-that-is-my-biopsy-maintaining-patient-identification/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From KSimeone at leavittmgt.com Mon Jul 25 09:26:51 2016 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Mon, 25 Jul 2016 14:26:51 +0000 Subject: [Histonet] FT OVERNIGHT HT POSTITION Delray Beach, FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CC9194C6C@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed HISTOTECHNOLOGIST here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Please fill out employment application HERE http://www.indeed.com/m/viewjob?jk=266fccd1fa688bb6&from=serp ^^you MUST follow this application link to apply! No exceptions. *full time position Mon-Fri OR Sun-Thurs 10p-6:30AM *MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo most of your shift) *MUST have at LEAST FIVE (5) years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE! *VERY proficient in embedding and microtomy *WILL MOSTLY BE EMBEDDING EXCISION BLOCKS, please know DERMS *must be self motivated, reliable and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) *some IHC experience preferred but not necessary Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor Delray Beach Technical Laboratory ADCS Clinics, LLC Advanced Dermatology and Cosmetic Surgery Ameriderm www.advancedderm.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From arunjyothisp at gmail.com Mon Jul 25 12:11:38 2016 From: arunjyothisp at gmail.com (Arun Jyothi S.P) Date: Mon, 25 Jul 2016 20:11:38 +0300 Subject: [Histonet] Formaldehyde and Xylene Detector Message-ID: Hi Readers Our lab would like to purchase formaldehyde and Xylene detectors as a part of accreditation. Please share the specification and manufacturer of the detectors that you are using or you know of. I look forward to hearing from you Thank you With Regards Arun Al Seef Hospital Kuwait From j.benavides at eae.csic.es Tue Jul 26 08:29:14 2016 From: j.benavides at eae.csic.es (Julio Benavides) Date: Tue, 26 Jul 2016 15:29:14 +0200 Subject: [Histonet] Formaldehyde and Xylene Detector In-Reply-To: References: Message-ID: <6e9e97d4-56e5-0040-0411-8dff0de2814c@eae.csic.es> I would also be very grateful if you could help me with this issue. We are trying to monitor xylene and formaldehyde levels in our lab, but we cannot find adequate measurement devices. What do you use? is there any equipment capable of give you an envoiromental level of these products in the way, in example, that there are digital thermometers which also measure the humidity and so on? Thanks for you help Cheers Julio El 25/07/2016 a las 19:11, Arun Jyothi S.P via Histonet escribi?: > Hi Readers > > Our lab would like to purchase formaldehyde and Xylene detectors as a part > of accreditation. > > Please share the specification and manufacturer of the detectors that you > are using or you know of. > > I look forward to hearing from you > > Thank you > With Regards > Arun > Al Seef Hospital > Kuwait > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock at mclean.harvard.edu Tue Jul 26 09:27:14 2016 From: twheelock at mclean.harvard.edu (Wheelock, Timothy R.) Date: Tue, 26 Jul 2016 14:27:14 +0000 Subject: [Histonet] Course of digital imaging? Message-ID: <69718C0B0B3C414D9F8E7214AD400CC99FBF297D@PHSX10MB11.partners.org> Good morning everyone: I have a DP-70 Olympus digital camera. Recently, I upgraded to a cellSens Standard software for the camera. Although some of my images, taken with the old Olympus software were gorgeous, many were not. I am also having problems with the new cellSens software. I feel that I need a real class or course on taking good microscopic digital images from histologic slides. Does anyone know of such a course, preferably in the Boston area? A course on the optics of a microscope would not hurt either. Thank you, Tim Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital, Belmont, MA Phone: 617-855-3592 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From TGoins at mt.gov Tue Jul 26 09:45:58 2016 From: TGoins at mt.gov (Goins, Tresa) Date: Tue, 26 Jul 2016 14:45:58 +0000 Subject: [Histonet] Formaldehyde and Xylene Detector In-Reply-To: References: Message-ID: <3a90ddf6a0f24b3f9e0133879d74ad12@doaisd5274.state.mt.ads> We have used two types: 1. Badges that are worn and then returned for analysis (Advanced Chemical Sensors, www.acsbadge.com has badges for both formaldehyde and xylene) and 2. Badges that contain a color indicator that require no analysis, just a visual check (Chromair from Morphix Technologies) Tresa -----Original Message----- From: Arun Jyothi S.P via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, July 25, 2016 11:12 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde and Xylene Detector Hi Readers Our lab would like to purchase formaldehyde and Xylene detectors as a part of accreditation. Please share the specification and manufacturer of the detectors that you are using or you know of. I look forward to hearing from you Thank you With Regards Arun Al Seef Hospital Kuwait _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght at yahoo.com Tue Jul 26 13:18:48 2016 From: tkngflght at yahoo.com (Cheryl) Date: Tue, 26 Jul 2016 11:18:48 -0700 Subject: [Histonet] pH of stainer waste water flow Message-ID: <150E1DAB-7A65-458B-AB07-A60E903514B7@yahoo.com> Hi guys- anyone know the overall pH of waste water coming off an automated stainer? Yeah, I know different models and solutions-- but a general response (acid/base/neutral) for a whole process would be helpful.... The city tested our run off and after 4+ years with the exact same process our waste is coming out waaaay too acidic. Help? Cheryl Kerry, HT(ASCP) Please excuse typos-sent from a phone. From esarricks at gmail.com Tue Jul 26 14:57:20 2016 From: esarricks at gmail.com (Erin Galati) Date: Tue, 26 Jul 2016 15:57:20 -0400 Subject: [Histonet] Microm Block Holder Message-ID: <6DCDF26D-1FFB-42B4-87A9-FA5C34F46967@gmail.com> Hi all, I am going to take a shot in the dark here and see if anyone has a block holder/chuck for a microm microtome that they would be willing to sell. Thanks in advance for your help! Regards, Erin From Danielle.Mark at aurora.org Tue Jul 26 15:21:52 2016 From: Danielle.Mark at aurora.org (Mark, Danielle) Date: Tue, 26 Jul 2016 20:21:52 +0000 Subject: [Histonet] PAS on Ventana Special Stainer Message-ID: I had wrote last week about crystals not rinsing off on our PAS slides on the Ventana special stainer. We had an application specialist come out and we discovered it was a bad lot. Thank you everyone for the input you gave. I appreciate the feedback. Danielle Mark, HT(ASCP), Technical Specialist ACL Laboratories 8901 W Lincoln Ave. | West Allis, WI 53227 O: 414.328.6235 | F: 414.328.6237 | Danielle.mark at aurora.org [https://mail.ahcmessaging.org/ecp/Customize/14.3.181.6/themes/resources/clear1x1.gif] www.acllaboratories.com From Pat.Patterson at propath.com Tue Jul 26 16:20:56 2016 From: Pat.Patterson at propath.com (Pat Patterson) Date: Tue, 26 Jul 2016 21:20:56 +0000 Subject: [Histonet] Dallas IHC Tech Position Message-ID: <6DCB8B92D0138244B56CE8EACE0D458D3EA9138D@Mail.propathlab.com> ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking a dedicated and experienced Histotechnician to join our Immunohistochemistry Laboratory to create quality IHC slides for the diagnosis of disease. The hours for this position are 3:00 p.m. to 11:30 p.m., Monday through Friday. Responsibilities include slide preparation (paraffin and frozen sections), IHC and In Situ hybridization staining, antibody titer preparation, pre-analytic paperwork and computer entry, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. The ideal candidate will have a minimum of 4 years' Histology experience with paraffin microtomy with a variety of different tissue types. Hands on IHC experience is strongly desired, but IHC training will be provided. HT (ASCP), HTL (ASCP), QIHC (ASCP) or BS desired. To apply, please visit www.propath.com Pat Patterson, HTL(ASCP) Manager, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From tawnia at ampianstaffing.com Tue Jul 26 16:37:09 2016 From: tawnia at ampianstaffing.com (Tawnia Lindsay) Date: Tue, 26 Jul 2016 21:37:09 +0000 Subject: [Histonet] Histology Job Opportunities Message-ID: It's a great time to FALL into a new opportunity. I have amazing jobs throughout the country; travel, temp to perm and direct hire. I have jobs in; Pennsylvania Washington State Nevada Missouri Oregon California And new ones opening up every day. Please call me if you are in the market for a new position, I would love to speak with you. Thanks, Tawnia Lindsay Senior Recruiter Ampian Staffing, Inc. 126 W Sego Lily Suite 110 Sandy UT 84070 O:877-229-6996 ext 2009 F:801-253-6127 email: tawnia at ampianstaffing.com Website: ampainstaffing.com From djmr55 at hotmail.com Tue Jul 26 17:40:41 2016 From: djmr55 at hotmail.com (donna mihalik rossi) Date: Tue, 26 Jul 2016 18:40:41 -0400 Subject: [Histonet] Crystals in PAS stain Message-ID: We are also having crystals show up on our PAS stain using the Ventana Benchmark . I was hoping using a new lot of stain would solve the problem but from previous posts I see this did not help. I will be contacting the company tomorrow. Anyone else having this issue? Thanks for your input. Donna Rossi, M.S. Hershey Medical Center of The Pennsylvania State University. From jford at cytomx.com Tue Jul 26 18:44:28 2016 From: jford at cytomx.com (Judi Ford) Date: Tue, 26 Jul 2016 23:44:28 +0000 Subject: [Histonet] Special stain art work Message-ID: Hi everyone, I am interested if anyone has used histology special stain/IHC/IF images as artwork for hallways. How large did you print the images, what resolution were they scanned at, etc. Years ago I came across an article about someone who created hall panels of special stain artwork. They created smaller image files at high resolution then stitched them together to create large (6'x6') panels to use in the hallways. Anyone remember seeing that article? Anyway, I would love hearing how people go about creating artwork with our images. Thanks, Judi CytomX Therapeutics South San Francisco, CA STATEMENT OF CONFIDENTIALITY: The information contained in this electronic message and any attachments to this message are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not an intended recipient, or a person responsible for delivering the e-mail to an intended recipient, you have received this message in error and that any use, dissemination, forwarding, printing, or copying is strictly prohibited. Please notify the sender at CytomX Therapeutics, Inc., immediately and destroy all copies of this message and any attachments. CytomX Therapeutics, Inc. From annigyg at gmail.com Tue Jul 26 22:45:38 2016 From: annigyg at gmail.com (Anne van Binsbergen) Date: Wed, 27 Jul 2016 07:45:38 +0400 Subject: [Histonet] Formaldehyde and Organic vapor monitors. Message-ID: <83ED6C2D-D273-4ABA-BFF0-620F598E35B6@gmail.com> Hi all Since 2006 we have used the badges from 3M. Purple (formalin) and brown (xylene and ethanol) colored badges with prepaid analysis included. We currently buy them off Amazon. CAP, JCIA and ISO 15189 are all happy with the process and results. I have 12 monitoring points in the lab and do an 8 hr measurement. Initially I measured 'people' but find it more effective to monitor high risk areas. We retest a minimum of every 2 years or if any additional 'offending' instruments are added or shifted. As per CAP. So far we have never been even close to an actionable PPM level. The badge assembly instructions are a bit fiddly but ok if the included brochure/data sheet is read carefully. Analysis is a breeze. We just pack them up and mail them to the US analysis lab who email the results on a pdf sheet. Any interested parties can contact me directly and I will email you screenshots of the the exact ones we use. Have a fabulous day. Anne Ps Of course we do have excellent ventilation/extraction. It helps Sent from my iPhone > On 26 Jul 2016, at 9:00 PM, histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Formaldehyde and Xylene Detector (Arun Jyothi S.P) > 2. Re: Formaldehyde and Xylene Detector (Julio Benavides) > 3. Course of digital imaging? (Wheelock, Timothy R.) > 4. Re: Formaldehyde and Xylene Detector (Goins, Tresa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 25 Jul 2016 20:11:38 +0300 > From: "Arun Jyothi S.P" > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Formaldehyde and Xylene Detector > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Hi Readers > > Our lab would like to purchase formaldehyde and Xylene detectors as a part > of accreditation. > > Please share the specification and manufacturer of the detectors that you > are using or you know of. > > I look forward to hearing from you > > Thank you > With Regards > Arun > Al Seef Hospital > Kuwait > > > ------------------------------ > > Message: 2 > Date: Tue, 26 Jul 2016 15:29:14 +0200 > From: Julio Benavides > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Formaldehyde and Xylene Detector > Message-ID: <6e9e97d4-56e5-0040-0411-8dff0de2814c at eae.csic.es> > Content-Type: text/plain; charset=windows-1252; format=flowed > > I would also be very grateful if you could help me with this issue. We > are trying to monitor xylene and formaldehyde levels in our lab, but we > cannot find adequate measurement devices. What do you use? is there any > equipment capable of give you an envoiromental level of these products > in the way, in example, that there are digital thermometers which also > measure the humidity and so on? > > Thanks for you help > > Cheers > > Julio > > > El 25/07/2016 a las 19:11, Arun Jyothi S.P via Histonet escribi?: >> Hi Readers >> >> Our lab would like to purchase formaldehyde and Xylene detectors as a part >> of accreditation. >> >> Please share the specification and manufacturer of the detectors that you >> are using or you know of. >> >> I look forward to hearing from you >> >> Thank you >> With Regards >> Arun >> Al Seef Hospital >> Kuwait >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 3 > Date: Tue, 26 Jul 2016 14:27:14 +0000 > From: "Wheelock, Timothy R." > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Course of digital imaging? > Message-ID: > <69718C0B0B3C414D9F8E7214AD400CC99FBF297D at PHSX10MB11.partners.org> > Content-Type: text/plain; charset="us-ascii" > > Good morning everyone: > > I have a DP-70 Olympus digital camera. > Recently, I upgraded to a cellSens Standard software for the camera. > Although some of my images, taken with the old Olympus software were gorgeous, many were not. > I am also having problems with the new cellSens software. > I feel that I need a real class or course on taking good microscopic digital images from histologic slides. > Does anyone know of such a course, preferably in the Boston area? > A course on the optics of a microscope would not hurt either. > > Thank you, > > Tim > > > Tim Wheelock > Harvard Brain Tissue Resource Center > McLean Hospital, Belmont, MA > Phone: 617-855-3592 > > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > ------------------------------ > > Message: 4 > Date: Tue, 26 Jul 2016 14:45:58 +0000 > From: "Goins, Tresa" > To: Arun Jyothi S.P , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Formaldehyde and Xylene Detector > Message-ID: <3a90ddf6a0f24b3f9e0133879d74ad12 at doaisd5274.state.mt.ads> > Content-Type: text/plain; charset="us-ascii" > > We have used two types: > > 1. Badges that are worn and then returned for analysis (Advanced Chemical Sensors, www.acsbadge.com has badges for both formaldehyde and xylene) and > 2. Badges that contain a color indicator that require no analysis, just a visual check (Chromair from Morphix Technologies) > > Tresa > > -----Original Message----- > From: Arun Jyothi S.P via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Monday, July 25, 2016 11:12 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Formaldehyde and Xylene Detector > > Hi Readers > > Our lab would like to purchase formaldehyde and Xylene detectors as a part of accreditation. > > Please share the specification and manufacturer of the detectors that you are using or you know of. > > I look forward to hearing from you > > Thank you > With Regards > Arun > Al Seef Hospital > Kuwait > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 152, Issue 23 > ***************************************** From srishan at mail.holyname.org Wed Jul 27 05:31:52 2016 From: srishan at mail.holyname.org (srishan at mail.holyname.org) Date: Wed, 27 Jul 2016 06:31:52 -0400 Subject: [Histonet] Histo tech qualifications Message-ID: Hi All, What is the basic education level required by CLIA, CAP to work in a Histology Lab. With the shrinkage in this field, searching for techs to work for you, does anyone know what the requirements are. I know the Histo Techs do not report out results to fall under the clinical lab requirements. Any suggestions will be appreciated. Thanks Nirmala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From amber.carson at thermofisher.com Wed Jul 27 12:01:56 2016 From: amber.carson at thermofisher.com (Carson, Amber G.) Date: Wed, 27 Jul 2016 17:01:56 +0000 Subject: [Histonet] Microm Block Holder In-Reply-To: <6DCDF26D-1FFB-42B4-87A9-FA5C34F46967@gmail.com> References: <6DCDF26D-1FFB-42B4-87A9-FA5C34F46967@gmail.com> Message-ID: Hi Erin, I am the Commercial Marketing Manager at Thermo Scientific over the sectioning product line. What block holder/chuck are you seeking? I can check in our inventory. Regards, Amber Amber Carson, MBA Commercial Marketing Manager, Sectioning Products Anatomical Pathology Thermo Fisher Scientific 4481 Campus Drive | Kalamazoo, MI? 49008? U.S.A. Tel: 1 (269) 544-5679 | Mobile 1 (269) 929-1748 amber.carson at thermofisher.com | www.thermoscientific.com/pathology -----Original Message----- From: Erin Galati [mailto:esarricks at gmail.com] Sent: Tuesday, July 26, 2016 3:57 PM To: Cheryl via Histonet Subject: [Histonet] Microm Block Holder Hi all, I am going to take a shot in the dark here and see if anyone has a block holder/chuck for a microm microtome that they would be willing to sell. Thanks in advance for your help! Regards, Erin From criley at dpspa.com Thu Jul 28 06:53:39 2016 From: criley at dpspa.com (Charles Riley) Date: Thu, 28 Jul 2016 07:53:39 -0400 Subject: [Histonet] Distilled Water vs DI water for IHC and H&E staining Message-ID: Does anyone know if there is a difference in staining results when using DI water versus Distilled water? Our lab is trying to get away from the DI system we have as it is becoming expensive to maintain and we were looking to see if we could just use a home drinking filter with UV lighting for our water supply. Please let me know any thoughts or concerns you might have with this idea -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From Bonnie.Whitaker at osumc.edu Thu Jul 28 07:00:44 2016 From: Bonnie.Whitaker at osumc.edu (Whitaker, Bonnie) Date: Thu, 28 Jul 2016 08:00:44 -0400 Subject: [Histonet] Distilled Water vs DI water for IHC and H&E staining In-Reply-To: References: Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670E31FC3E48@EXMBOX-VP05.OSUMC.EDU> Hi Charles, You will need to give it a try, in order to know for sure. Water quality is EXTREMELY variable, from community to community, and even the pipes, etc., within in your building can influence the water quality. The chemicals used by the local water district can also vary, based upon the season, and your geographical location. Good luck! Bonnie Sent with Good (www.good.com) Bonnie -----Original Message----- From: Charles Riley via Histonet [histonet at lists.utsouthwestern.edu] Sent: Thursday, July 28, 2016 07:54 AM Eastern Standard Time To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Distilled Water vs DI water for IHC and H&E staining Does anyone know if there is a difference in staining results when using DI water versus Distilled water? Our lab is trying to get away from the DI system we have as it is becoming expensive to maintain and we were looking to see if we could just use a home drinking filter with UV lighting for our water supply. Please let me know any thoughts or concerns you might have with this idea -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=CwICAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=n8R816TJlc5EYpeIhpwg-Fjrc1pwHWhL9T4g_fpHsK0&s=PLr-H4l_tOWZcByk_tnh7feVsezk0OTBCpPzAQqyC0I&e= From twheelock at mclean.harvard.edu Thu Jul 28 08:25:43 2016 From: twheelock at mclean.harvard.edu (Wheelock, Timothy R.) Date: Thu, 28 Jul 2016 13:25:43 +0000 Subject: [Histonet] Distilled Water vs DI water for IHC and H&E staining In-Reply-To: References: Message-ID: <69718C0B0B3C414D9F8E7214AD400CC99FBF2F03@PHSX10MB11.partners.org> Hi Charles: Many years ago I was using deionized water to do the Bielschowsky silver stain. The stains were coming out way too dark, with black-brown precipitate on the sections. When I inherited a double-distilled water filtration system, that problem disappeared. However, silver stains tend to be sensitive to all sorts of things. I don't know if the same holds true for immunostains, since I don't do them myself (another lab does them for us). When I do my Luxol Fast Blue (myelin)-Hematoxylin-Eosin stain, I just use tap water or all my washes. I make my solutions for the stain with double-distilled water, but I am not sure if that is necessary. I hope that this helps. Tim Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 28, 2016 7:54 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Distilled Water vs DI water for IHC and H&E staining Does anyone know if there is a difference in staining results when using DI water versus Distilled water? Our lab is trying to get away from the DI system we have as it is becoming expensive to maintain and we were looking to see if we could just use a home drinking filter with UV lighting for our water supply. Please let me know any thoughts or concerns you might have with this idea -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From eunicemdelapena at yahoo.com Thu Jul 28 10:43:14 2016 From: eunicemdelapena at yahoo.com (Eunice) Date: Thu, 28 Jul 2016 10:43:14 -0500 Subject: [Histonet] Job open Message-ID: <215A227D-E8ED-4E49-AF4F-9240E2A6BF07@yahoo.com> Swedish Covenant hospital in Chicago has a 20 hr evening position open from 6pm to 10pm mon to fri ... And possibly another every other Saturday open .... 430am to 100pm please contact Ruth Cazares at 773 878 8200 ex 5190 Sent from my iPhone From nlinke at sbch.org Thu Jul 28 11:14:45 2016 From: nlinke at sbch.org (Noelle Linke) Date: Thu, 28 Jul 2016 16:14:45 +0000 Subject: [Histonet] paraffin temp-processing Message-ID: Hi all, We are using Parapast X-tra on the Peloris at 65C (factory default), and I am thinking this is just way too hot (X-tra has a melting point of 52C). Leica of course will not commit to an opinion. Is anyone else using paraplast x-tra on their Peloris? Thank you, No?lle No?lle Linke, MS, HTL(ASCP) QIHC Manager, Anatomic Pathology Pacific Diagnostic Laboratories nlinke at sbch.org Phone: (805) 324-9814 Fax: (805) 696-6433 ________________________________ CH Disclaimer: This electronic mail message is intended exclusively for the individual or entity to which it is addressed. This message, together with any attachment, may contain confidential and privileged information. Any views, opinions or conclusions expressed in this message are those of the individual sender and do not necessarily reflect the views of Cottage Health, its subsidiaries or affiliates. This document may also contain information covered under the Health Insurance Portability and Accountability Act (HIPAA, PL 104-191) and implementing regulations and must be protected in accordance with those provisions. Re-disclosure without patient consent or as otherwise permitted by law is prohibited. Any unauthorized review, retransmission, use, printing, copying, retention, disclosure, distribution or the taking of any action in reliance upon this information by persons or entities other than the intended recipient is strictly prohibited and may be unlawful. If you have received this message in error, please immediately advise the sender by reply email message to the sender and delete all copies of this message from your system without copying. ________________________________ From Timothy.Morken at ucsf.edu Thu Jul 28 11:54:01 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 28 Jul 2016 16:54:01 +0000 Subject: [Histonet] Distilled Water vs DI water for IHC and H&E staining In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF6FD738A3@ex07.net.ucsf.edu> Charles, for most histology applications water from laboratory-grade DI and distilled systems are fairly interchangeable because modern systems bring them pretty close to the same purity. But some stains can be affected if they are susceptible to chemical interactions with something left in the water. That is probably more important for tests like enzyme histochemistry than bulk chemical dye staining. These systems are not just stand-alone "deionized" or "distilled" anymore and now include various pre- and post- filtration steps for removing particulates, volatile compounds, bacteria, etc (ours "DI" system has several DI resin beds, carbon filters, particulate filters, UV etc). Even modern distilling systems will filter tap water with various filters (particulates, carbon, bacteria) before distilling. I don't think the consumer type filters will do the same job as a DI or distilled system. They are designed to filter out only the particulates and volatile compounds (particulate/bacteria and carbon filters for the most part). There is no deionization or demineralization (assuming you are using city tap water). However, a reverse osmosis system (which also has several other filters pre-RO) may do the trick for you. It is not quite as good at purifying as a laboratory-quality DI or Distilled system, but may suffice for histology. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 28, 2016 4:54 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Distilled Water vs DI water for IHC and H&E staining Does anyone know if there is a difference in staining results when using DI water versus Distilled water? Our lab is trying to get away from the DI system we have as it is becoming expensive to maintain and we were looking to see if we could just use a home drinking filter with UV lighting for our water supply. Please let me know any thoughts or concerns you might have with this idea -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mills at 3scan.com Thu Jul 28 12:29:18 2016 From: mills at 3scan.com (Caroline Miller) Date: Thu, 28 Jul 2016 10:29:18 -0700 Subject: [Histonet] Distilled Water vs DI water for IHC and H&E staining In-Reply-To: <761E2B5697F795489C8710BCC72141FF6FD738A3@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF6FD738A3@ex07.net.ucsf.edu> Message-ID: Totally agree with the other comments, I had one anecdote though. I worked in a clinical lab at the Hammersmith in London, with an autostainer. One day the H&E started coming off really funny, way too blue, not enough eosin. We traced everything in the lab, and NOTHING had changed. We scratched our heads, banged them against the wall and fixed the problem by changing staining times and fiddling with the protocol. JUST as we got it fixed the powers that be told us the whole hospital had changed our main water supply! Oh, histology, you are so voodoo sometimes! Happy Friday! mills On Thu, Jul 28, 2016 at 9:54 AM, Morken, Timothy via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Charles, for most histology applications water from laboratory-grade DI > and distilled systems are fairly interchangeable because modern systems > bring them pretty close to the same purity. But some stains can be affected > if they are susceptible to chemical interactions with something left in the > water. That is probably more important for tests like enzyme histochemistry > than bulk chemical dye staining. > > These systems are not just stand-alone "deionized" or "distilled" anymore > and now include various pre- and post- filtration steps for removing > particulates, volatile compounds, bacteria, etc (ours "DI" system has > several DI resin beds, carbon filters, particulate filters, UV etc). Even > modern distilling systems will filter tap water with various filters > (particulates, carbon, bacteria) before distilling. > > I don't think the consumer type filters will do the same job as a DI or > distilled system. They are designed to filter out only the particulates and > volatile compounds (particulate/bacteria and carbon filters for the most > part). There is no deionization or demineralization (assuming you are using > city tap water). However, a reverse osmosis system (which also has several > other filters pre-RO) may do the trick for you. It is not quite as good at > purifying as a laboratory-quality DI or Distilled system, but may suffice > for histology. > > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > > -----Original Message----- > From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu > ] > Sent: Thursday, July 28, 2016 4:54 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Distilled Water vs DI water for IHC and H&E staining > > Does anyone know if there is a difference in staining results when using > DI water versus Distilled water? Our lab is trying to get away from the DI > system we have as it is becoming expensive to maintain and we were looking > to see if we could just use a home drinking filter with UV lighting for our > water supply. Please let me know any thoughts or concerns you might have > with this idea > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From tbraud at holyredeemer.com Thu Jul 28 14:05:34 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 28 Jul 2016 19:05:34 +0000 Subject: [Histonet] It's getting cold in here.... Message-ID: <48E053DDF6CE074DB6A7414BA05403F8082AC9@HRHEX02-HOS.holyredeemer.local> If you've not been made aware before now, be warned. The Department of Veterans affairs (DVA) has proposed a rule that would authorize any Advanced Practice Registered Nurse (APRN) to not only order and interpret lab tests, but also to perform, supervise, and direct lab testing. The rule follows lab personnel requirement changes enacted by the Centers for Medicare and Medicaid Services (CMS) that say that a bachelor's or associate's degree in nursing is sufficient to perform high complexity lab testing. With a nod to nursing degrees that I'm sure prepare a nurse extremely well for nursing, there is no way that a nursing degree can adequately prepare a person to perform complex laboratory testing. Unless your state has technologist licensure, and few do, get prepared for nurses running the lab, and lab tests. And where will that leave the histologist technician? I'm guessing out in the cold. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 ********************************** From Timothy.Morken at ucsf.edu Thu Jul 28 14:26:00 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 28 Jul 2016 19:26:00 +0000 Subject: [Histonet] It's getting cold in here.... In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8082AC9@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8082AC9@HRHEX02-HOS.holyredeemer.local> Message-ID: <761E2B5697F795489C8710BCC72141FF6FD73990@ex07.net.ucsf.edu> Seems like nurses would have enough to do already! I wonder if this is aimed at physician labs where they may have a nurse but no lab tech... California requirement for RN BS degree require only one quarter of microbiology with lab, and one quarter of basic chemistry with lab. And 3 anatomy/physiology quarter courses Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center Tim -----Original Message----- From: Terri Braud via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 28, 2016 12:06 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] It's getting cold in here.... If you've not been made aware before now, be warned. The Department of Veterans affairs (DVA) has proposed a rule that would authorize any Advanced Practice Registered Nurse (APRN) to not only order and interpret lab tests, but also to perform, supervise, and direct lab testing. The rule follows lab personnel requirement changes enacted by the Centers for Medicare and Medicaid Services (CMS) that say that a bachelor's or associate's degree in nursing is sufficient to perform high complexity lab testing. With a nod to nursing degrees that I'm sure prepare a nurse extremely well for nursing, there is no way that a nursing degree can adequately prepare a person to perform complex laboratory testing. Unless your state has technologist licensure, and few do, get prepared for nurses running the lab, and lab tests. And where will that leave the histologist technician? I'm guessing out in the cold. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 ********************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abeharry798 at gmail.com Thu Jul 28 18:42:25 2016 From: abeharry798 at gmail.com (Gmail) Date: Thu, 28 Jul 2016 19:42:25 -0400 Subject: [Histonet] Feedback on Immunohistochemistry stainers Message-ID: <3580D943-0B0E-4A3A-B640-B6BCC8B17442@gmail.com> Hi all, We are looking into getting a new staining platform for our IHC lab. I would appreciate any feedback from your experience regarding ease of use, how long maintenance;daily , monthly takes to perform and through put. Are there any issues you ran into ? We run approximately 4-6000 slides/ month and are looking into Roche, Leica and Dako. Thanks in advance Andrea Beharry Senior MLT, Immunohistochemistry William Osler Health System From relia1 at earthlink.net Fri Jul 29 08:35:10 2016 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 29 Jul 2016 09:35:10 -0400 Subject: [Histonet] Christmas in July? Message-ID: <00a201d1e99e$074f30f0$15ed92d0$@earthlink.net> Hi Histonetters! How are you? I hope you have a great weekend! It's Christmas in July! First of all THANK YOU for taking the time to read my post. Every couple of weeks I post a career bulletin listing all of my current openings and I appreciate the space you allow me on the forum and in your inbox. I do have some positions to tell you about today as well but more importantly I want to tell you about RELIA's referral program. If you refer someone to RELIA and we place them you will earn a referral fee of $250.00. Wouldn't it be great to have a referral bonus to your Christmas shopping budget? That's what I mean by Christmas in July. Refer someone today and have more Christmas money later! What do you think? Here is a list of the areas where some of our hottest and most exciting opportunities are located. RELIA's Current Histology Opportunities: Texas Virginia California Arizona Arkansas Michigan Alabama Tennessee With new positions from across the nation coming in on a daily basis!! All of my clients offer excellent compensation, benefits and some offer relocation assistance and or sign on bonuses. All of these jobs are full time & permanent & most of them are RELIA Exclusives!!! So if you or someone you know might be interested in any of these areas or are considering a job search in another area please contact me. I would be happy to help! I can be reached ASAP via email at relia1 at earthlink.net or toll free at the office at 866-607-3542 or on my cell at 407-353-5070 call or text! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From rjbuesa at yahoo.com Fri Jul 29 09:10:19 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 29 Jul 2016 14:10:19 +0000 (UTC) Subject: [Histonet] Feedback on Immunohistochemistry stainers In-Reply-To: <3580D943-0B0E-4A3A-B640-B6BCC8B17442@gmail.com> References: <3580D943-0B0E-4A3A-B640-B6BCC8B17442@gmail.com> Message-ID: <1679166132.6246173.1469801419975.JavaMail.yahoo@mail.yahoo.com> Request a DAKO demo.Ren? On Thursday, July 28, 2016 8:01 PM, Gmail via Histonet wrote: Hi all, We are looking into getting a new staining platform for our IHC lab. I would appreciate any feedback from your experience regarding ease of use, how long maintenance;daily , monthly takes to perform and through put. Are there any issues you ran into ? We run approximately 4-6000 slides/ month and are looking into Roche, Leica and Dako. Thanks in advance Andrea Beharry Senior MLT, Immunohistochemistry William Osler Health System _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robert.jacox at thermofisher.com Fri Jul 29 10:10:00 2016 From: robert.jacox at thermofisher.com (Jacox, Robert A.) Date: Fri, 29 Jul 2016 15:10:00 +0000 Subject: [Histonet] Distilled Water vs DI water for IHC and H&E staining In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF6FD738A3@ex07.net.ucsf.edu>, Message-ID: Caroline, As a vendor I have had the privilege to visit several hundred labs throughout the United States and in other countries. From what I have seen, I would say the the answer to your question is it depends on the quality of your local water source. In most cases city water systems a rather stable. With that said though I can remember one lab that would call me twice a year to complain about their staining quality. We were able to tie it back to when their city would clean their lines. The chemicals the city used removed almost all the Hematoxylin. So, I would advise if you are seeing variation and think it's your water make sure you are in contact with your city. Good luck Robert Sent from my iPad > On Jul 28, 2016, at 6:49 PM, Caroline Miller via Histonet wrote: > > Totally agree with the other comments, I had one anecdote though. I worked > in a clinical lab at the Hammersmith in London, with an autostainer. One > day the H&E started coming off really funny, way too blue, not enough > eosin. We traced everything in the lab, and NOTHING had changed. We > scratched our heads, banged them against the wall and fixed the problem by > changing staining times and fiddling with the protocol. > > JUST as we got it fixed the powers that be told us the whole hospital had > changed our main water supply! > > Oh, histology, you are so voodoo sometimes! > > Happy Friday! > > mills > > On Thu, Jul 28, 2016 at 9:54 AM, Morken, Timothy via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Charles, for most histology applications water from laboratory-grade DI >> and distilled systems are fairly interchangeable because modern systems >> bring them pretty close to the same purity. But some stains can be affected >> if they are susceptible to chemical interactions with something left in the >> water. That is probably more important for tests like enzyme histochemistry >> than bulk chemical dye staining. >> >> These systems are not just stand-alone "deionized" or "distilled" anymore >> and now include various pre- and post- filtration steps for removing >> particulates, volatile compounds, bacteria, etc (ours "DI" system has >> several DI resin beds, carbon filters, particulate filters, UV etc). Even >> modern distilling systems will filter tap water with various filters >> (particulates, carbon, bacteria) before distilling. >> >> I don't think the consumer type filters will do the same job as a DI or >> distilled system. They are designed to filter out only the particulates and >> volatile compounds (particulate/bacteria and carbon filters for the most >> part). There is no deionization or demineralization (assuming you are using >> city tap water). However, a reverse osmosis system (which also has several >> other filters pre-RO) may do the trick for you. It is not quite as good at >> purifying as a laboratory-quality DI or Distilled system, but may suffice >> for histology. >> >> >> Tim Morken >> Pathology Site Manager, Parnassus >> Supervisor, Electron Microscopy/Neuromuscular Special Studies >> Department of Pathology >> UC San Francisco Medical Center >> >> >> >> >> -----Original Message----- >> From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu >> ] >> Sent: Thursday, July 28, 2016 4:54 AM >> To: histonet at lists.utsouthwestern.edu >> Subject: [Histonet] Distilled Water vs DI water for IHC and H&E staining >> >> Does anyone know if there is a difference in staining results when using >> DI water versus Distilled water? Our lab is trying to get away from the DI >> system we have as it is becoming expensive to maintain and we were looking >> to see if we could just use a home drinking filter with UV lighting for our >> water supply. Please let me know any thoughts or concerns you might have >> with this idea >> >> -- >> >> Charles Riley HT(ASCP)CM >> >> Histopathology Coordinator/ Mohs >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Caroline Miller (mills) > Director of Histology > 3Scan.com > 415 2187297 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abadesuyi at nrh-ok.com Fri Jul 29 13:46:19 2016 From: abadesuyi at nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Fri, 29 Jul 2016 13:46:19 -0500 Subject: [Histonet] Automated Rotary Microtome Message-ID: <04EE4F75BB5FB246ADB68D69B7460443AFCB1E9C30@MAIL.nrhnt.nrh-ok.com> Hi, We are in the process of purchasing Automated Rotary Microtomes and I will appreciate recommendations from you guys. Have a great weekend. Sincerely, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 Cell: 405-973-6363 abadesuyi at nrh-ok.com ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From corvodiva at gmail.com Fri Jul 29 14:48:09 2016 From: corvodiva at gmail.com (jenny sandy) Date: Fri, 29 Jul 2016 12:48:09 -0700 Subject: [Histonet] PFA Fixed tissue not sticking to slides? Message-ID: Dear HistoFolks, Has anyone had experience with cryo-sectioned tissue that is PFA fixed not adhering to slides? The tissue is rabbit cornea culture, 4% PFA fixed O/N, then run through 20% sucrose gradient, then frozen in OCT. We think that the PFA fix is much longer than needed for a tissue this thin, and were going to try 2 hr fix. Would over-fixation cause the section to not adhere? The PFA/Sucrose fixation really improves the morphology and the IHC stains that we have run so far. Thank you in advance for your help. Jennifer Eveleth From liz at premierlab.com Fri Jul 29 14:56:58 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Fri, 29 Jul 2016 13:56:58 -0600 Subject: [Histonet] PFA Fixed tissue not sticking to slides? In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02BED535B4E6@SBS2K8.premierlab.local> Are you using a good plus slide? We have started using these Tru-Bond slides when we have tissue adherence problems, they seem to work really well. How are you handling the slides once you section? Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: jenny sandy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, July 29, 2016 1:48 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] PFA Fixed tissue not sticking to slides? Dear HistoFolks, Has anyone had experience with cryo-sectioned tissue that is PFA fixed not adhering to slides? The tissue is rabbit cornea culture, 4% PFA fixed O/N, then run through 20% sucrose gradient, then frozen in OCT. We think that the PFA fix is much longer than needed for a tissue this thin, and were going to try 2 hr fix. Would over-fixation cause the section to not adhere? The PFA/Sucrose fixation really improves the morphology and the IHC stains that we have run so far. Thank you in advance for your help. Jennifer Eveleth _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jillian.russell at agilent.com Fri Jul 29 15:26:34 2016 From: jillian.russell at agilent.com (jillian.russell at agilent.com) Date: Fri, 29 Jul 2016 20:26:34 +0000 Subject: [Histonet] B-5 Fixative Message-ID: Hello! I am wondering how many labs are using B-5 fixatives and how many are using B-5 alternatives due to the mercury issue? For those who have switched to an alternative, have you noticed many differences from the traditional B-5 fixative? Any and all feedback would be greatly appreciated! :) Thank you, Jillian A. Russell Jillian A. Russell, HT (ASCP)CM, QIHCCM Supervisor, CDx Histology Operations, R&D Diagnostics & Genomics Dako North America, Inc., an Agilent Technologies Company 6392 Via Real | Carpinteria | CA 93013 | USA From Timothy.Morken at ucsf.edu Fri Jul 29 16:12:01 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 29 Jul 2016 21:12:01 +0000 Subject: [Histonet] Question about prelim report for autopsy with neuropathology Message-ID: <761E2B5697F795489C8710BCC72141FF6FD746E4@ex07.net.ucsf.edu> How do you handle counting days to preliminary autopsy dx (PAD) when doing cases in which the brain autopsy results are the main component of disease? If we accession at the time of autopsy and then let the brain fix for several days before cutting in, then the PAD is many days past the 3-day reporting requirement. On standard "body" cases we report PAD the same day and final within two weeks. With neuropathology cases it can be up to 10 days for a PAD. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From Megan.Dishop at childrensmn.org Fri Jul 29 16:35:27 2016 From: Megan.Dishop at childrensmn.org (Megan Dishop) Date: Fri, 29 Jul 2016 16:35:27 -0500 Subject: [Histonet] Question about prelim report for autopsy with neuropathology In-Reply-To: <761E2B5697F795489C8710BCC72141FF6FD746E4@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF6FD746E4@ex07.net.ucsf.edu> Message-ID: <579B85CF.D202.00E3.1@childrensmn.org> Hi Tim, In the hospitals I have worked, we used the PAD simply to communicate gross pathologic diagnoses, so we would issue a gross description of the brain (like the brain weight compared to normal, edema, atrophy, herniation, hemorrhage, etc), "pending microscopic examination", and then follow with the FAD after microscopic exam is done. It might just be a few lines, but that's OK. It gives the clinical team a quick update of what was observed and what is pending. Megan Megan K. Dishop MD Medical Director, Pediatric Anatomic Pathology Children's Hospitals and Clinics of Minnesota Laboratories 2525 Chicago Ave S. MS32-B600, Minneapolis, MN 55404 USA Phone: 612-813-6521 Fax: 612-813-7721 Email: megan.dishop at childrensMN.org Adjoint Professor of Pediatrics, University of Colorado School of Medicine >>> "Morken, Timothy via Histonet" 7/29/2016 4:12 PM >>> How do you handle counting days to preliminary autopsy dx (PAD) when doing cases in which the brain autopsy results are the main component of disease? If we accession at the time of autopsy and then let the brain fix for several days before cutting in, then the PAD is many days past the 3-day reporting requirement. On standard "body" cases we report PAD the same day and final within two weeks. With neuropathology cases it can be up to 10 days for a PAD. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. From corvodiva at gmail.com Fri Jul 29 17:40:15 2016 From: corvodiva at gmail.com (jenny sandy) Date: Fri, 29 Jul 2016 15:40:15 -0700 Subject: [Histonet] PFA Fixed Tissue not sticking to slides? Message-ID: <9AD02EFB-BDA5-41D9-BB7D-625CF3AB1DCE@gmail.com> Thank you Charles Scouton for responding. The slides are positive charged slides from IMEB. Thank you Elizabeth Chilpala for responding. The treatment after sectioning is one I?ve forwarded to the service that is processing for us. I only do the PFA/sucrose/embedding part. My understanding is it is pretty standard (?) for cryo sections for H&E and IHC, but I am checking that out. Jennifer Eveleth Original message below: Dear HistoFolks, Has anyone had experience with cryo-sectioned tissue that is PFA fixed not adhering to slides? The tissue is rabbit cornea culture, 4% PFA fixed O/N, then run through 20% sucrose gradient, then frozen in OCT. We think that the PFA fix is much longer than needed for a tissue this thin, and were going to try 2 hr fix. Would over-fixation cause the section to not adhere? The PFA/Sucrose fixation really improves the morphology and the IHC stains that we have run so far. Thank you in advance for your help. Jennifer Eveleth From carl.hobbs at kcl.ac.uk Sat Jul 30 13:04:34 2016 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sat, 30 Jul 2016 18:04:34 +0000 Subject: [Histonet] PFA Fixed tissue not sticking to slides? Message-ID: Hi Jenny. Can you use Pwax embedding as an alternative? H&E will always be better on Pwax sections. IHC will also be in many instances. What abs are you probing with? Single/double-labelling? Yes....you are fixing for too long ( for frozen sectioning....for Pwax you can fix your specimens for a week and still not suffer IHC-problems: after HIER they will or will never work, depending upon ab) PFA( Formalin) fixed sections are well-known for not being as adherent as unfixed frozen material. Why not freeze unfixed? No sucrose required ( all that does is allow for slow freezing without, in most cases, formation of ice-crystals: good snap freezing does not need sucrose pretreatment) If you are after good morphology then Pwax is the way to go, imho. If you have to cut PFA-fixed then frozen tissues, after mounting on slides stick them in a 50C oven for 2hrs. Use Superfrost Plus slides. Then use or, store at 4C for a week or freeze. For the latter 2, ALWAYS take out of fridge/freezer and immediately place under a fan/operating fume hood. Do not allow condensation to form. Or....place them immediately into that same 50C oven for an hour. Do not do this to unfixed frozen sections! Sure, check out EC's excellent advice ( as always) re slides. Interestedly, Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From craigak12 at gmail.com Sat Jul 30 14:03:12 2016 From: craigak12 at gmail.com (J B) Date: Sat, 30 Jul 2016 19:03:12 +0000 Subject: [Histonet] FLO.30640: Message-ID: CAP says that I need to have a procedure for Flow cytometry interpretation. I have asked our reference laboratory for the procedure & they will not help. Does anyone have a flow cytometry interpretation procedure that they can share? Sincerely, JB From michang2014 at gmail.com Sat Jul 30 14:29:58 2016 From: michang2014 at gmail.com (Michelle Chang) Date: Sat, 30 Jul 2016 12:29:58 -0700 Subject: [Histonet] PFA Fixed tissue not sticking to slides? Message-ID: <80CA0F03-7E17-4ACC-BA1D-9620820DD016@gmail.com> Good charged slides or subbed slides will work. In regards to the fixation, I personally find the morphology with 4% PFA O/N followed by 30% sucrose better than doing them simultaneously. Michelle From carl.hobbs at kcl.ac.uk Sun Jul 31 14:06:29 2016 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sun, 31 Jul 2016 19:06:29 +0000 Subject: [Histonet] PFA Fixed tissue not sticking to slides? Message-ID: No...never add sucrose to "PFA" when fixing tissues for freezing....or any other time. Fix, then sucrose- immerse until tissue sinks to bottom of receptacle.... if you can't snap-freeze effectively Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From rsrichmond at gmail.com Sun Jul 31 16:43:20 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Sun, 31 Jul 2016 17:43:20 -0400 Subject: [Histonet] B-5 Fixative Message-ID: Jillian A. Russell, HT (ASCP)CM, QIHCCM, Supervisor, CDx Histology Operations, R&D at Dako in Carpinteria CA asks: >>I am wondering how many labs are using B-5 fixatives and how many are using B-5 alternatives due to the mercury issue? - For those who have switched to an alternative, have you noticed many differences from the traditional B-5 fixative?<< This is a very controversial topic. B-5 fixative contains a large quantity of mercuric chloride, along with formaldehyde, and you really can't use it any more. I gave up B-5 and Zenker's fixatives very reluctantly. My personal opinion is that B-5 substitutes are no improvement on neutral buffered formalin, and may compromise immunostains (which often specify NBF fixation). Bob Richmond Samurai Pathologist Maryville TN