From histotech at imagesbyhopper.com Sat Jan 2 16:02:47 2016 From: histotech at imagesbyhopper.com (Michelle) Date: Sat, 2 Jan 2016 17:02:47 -0500 Subject: [Histonet] CLIA regs question In-Reply-To: <469FD9C7F82DC749A414F241CB0474671FADAE1F@EXCHANGE02.ohpin.com> References: <7AA54CE5-6F8E-4256-B6BD-7DBB800F7369@imagesbyhopper.com> <469FD9C7F82DC749A414F241CB0474671FADAE1F@EXCHANGE02.ohpin.com> Message-ID: <010601d145a9$541532c0$fc3f9840$@imagesbyhopper.com> I just wanted to say thanks to all who assisted me in my goal of getting a lab CLIA certified. They had their inspection and just before Christmas they received their CLIA number. They got through their inspection with zero deficiencies! :) Thanks again, Michelle From tkngflght at yahoo.com Sun Jan 3 20:25:05 2016 From: tkngflght at yahoo.com (Cheryl) Date: Mon, 4 Jan 2016 02:25:05 +0000 (UTC) Subject: [Histonet] Day shift with sign-on bonus in the Carolinas! References: <249629325.6508064.1451874305997.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <249629325.6508064.1451874305997.JavaMail.yahoo@mail.yahoo.com> Hi Everyone-- tired of this cold weather? ?Want to work a not-too-early day shift? Open day shift position in South Carolina. ?Don't rule it out -- if you're curious give me a call. ?Complete with sign-on bonus and a good benefit package. Send a current resume or give me a call - Cheryl?Cheryl Kerry, HT(ASCP) Full Staff Inc. ? admin at fullstaff.org?800.756.3309 Phone & Fax https://www.facebook.com/TheHistologyCompany/ From mbmphoto at gmail.com Sun Jan 3 22:28:58 2016 From: mbmphoto at gmail.com (Maria Mejia) Date: Sun, 3 Jan 2016 20:28:58 -0800 Subject: [Histonet] Help with causes or theory behind failure of nuclear counterstaining after IHC! Message-ID: Happy New Year Everyone, I'm the lead histologist working in an IHC based research lab focused on early stages of Alzheimer's disease. We work on paraffin sections processed & cut from 600um celloidin sections. Including a lot of 60um cellodin sections from whole human brainstem. For years, everything has going good regarding counterstaining after single & double IHC staining on 60um free-floating sections. However for the past two months we've struggled to achieve good visible counterstaining on IHC sections to count the stained neurons - to see clearly the nucleus & nucleolus! For a number of years, Gallocyanine was our choice of counterstain after IHC. Now, it's NOT working (neurons not stained visible enough to count). We've also tried cresyl violet counterstain - staining too weak! In both counterstains, we modified the staining protocols quite a number of times to get good visible staining - nothing!!! Strangle because we get lovely counterstained neurons with NO IHC staining on our 60um sections, but as soon as we take the sections through the IHC protocol e.g. antigen retrieval, antibodies & chromogens - counterstain is too weak! Our paraffin IHC sections work & look wonderful! Now, my PI wants to try methyl green counterstain, however I think we'll have the same problem. Here's what I need help with: 1) Can someone please explain the reason or theory behind the failure of counterstain uptake by cells such as human neurons on 60um celloidin sections? 2) Can anyone please offer staining protocols that use alternative dehydration & clearing reagents. I've been using alcohols dehydration (96% & 100%) without success as well as clearing with xylene - which hardens the tissue if left too long in this reagent. I was thinking of perhaps using acetone instead of alcohols & maybe using a methyl salicylate or chloroform. Thoughts anyone? I wish Dr Chris van der Loos was still with us. I'd dearly like to hear from anyone who can help with this issue - Gayle Callis, Terry Johnson, Dr Hohn Kiernan et al. Any assistance anyone can provide will be greatly appreciated! Best Maria Mejia UCSF Memory & Aging Department San Francisco, CA From amurvosh at advancederm.net Mon Jan 4 09:11:14 2016 From: amurvosh at advancederm.net (Anne Murvosh) Date: Mon, 4 Jan 2016 15:11:14 +0000 Subject: [Histonet] Height regulations Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7ADDA56@Exchange.Advancederm.net> Putting in some lower shelving for storage and I can't remember the height regulation for keeping stuff off the floor. I tried to google it, it must be buried in some government doc. Please Help? Thanks Anne From Timothy.Morken at ucsf.edu Mon Jan 4 09:54:12 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 4 Jan 2016 15:54:12 +0000 Subject: [Histonet] Help with causes or theory behind failure of nuclear counterstaining after IHC! In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF603455A7@ex07.net.ucsf.edu> Maria, If the counterstain is good when done before IHC stain and poor after it sounds like proteins are being extracted during the IHC processing and staining. Have you tried staining sections after each step of the IHC process to isolate the point the stain becomes weak? Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Maria Mejia via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Sunday, January 03, 2016 8:29 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Help with causes or theory behind failure of nuclear counterstaining after IHC! Happy New Year Everyone, I'm the lead histologist working in an IHC based research lab focused on early stages of Alzheimer's disease. We work on paraffin sections processed & cut from 600um celloidin sections. Including a lot of 60um cellodin sections from whole human brainstem. For years, everything has going good regarding counterstaining after single & double IHC staining on 60um free-floating sections. However for the past two months we've struggled to achieve good visible counterstaining on IHC sections to count the stained neurons - to see clearly the nucleus & nucleolus! For a number of years, Gallocyanine was our choice of counterstain after IHC. Now, it's NOT working (neurons not stained visible enough to count). We've also tried cresyl violet counterstain - staining too weak! In both counterstains, we modified the staining protocols quite a number of times to get good visible staining - nothing!!! Strangle because we get lovely counterstained neurons with NO IHC staining on our 60um sections, but as soon as we take the sections through the IHC protocol e.g. antigen retrieval, antibodies & chromogens - counterstain is too weak! Our paraffin IHC sections work & look wonderful! Now, my PI wants to try methyl green counterstain, however I think we'll have the same problem. Here's what I need help with: 1) Can someone please explain the reason or theory behind the failure of counterstain uptake by cells such as human neurons on 60um celloidin sections? 2) Can anyone please offer staining protocols that use alternative dehydration & clearing reagents. I've been using alcohols dehydration (96% & 100%) without success as well as clearing with xylene - which hardens the tissue if left too long in this reagent. I was thinking of perhaps using acetone instead of alcohols & maybe using a methyl salicylate or chloroform. Thoughts anyone? I wish Dr Chris van der Loos was still with us. I'd dearly like to hear from anyone who can help with this issue - Gayle Callis, Terry Johnson, Dr Hohn Kiernan et al. Any assistance anyone can provide will be greatly appreciated! Best Maria Mejia UCSF Memory & Aging Department San Francisco, CA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac at outlook.com Mon Jan 4 13:38:56 2016 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Mon, 4 Jan 2016 12:38:56 -0700 Subject: [Histonet] Help with causes or theory behind failure of nuclear counterstaining after IHC! Message-ID: Maria, I. Think you may have a pH issue. High pH results in reduction of protons, H+, effects dye structure and can cause light to no staining after bluing. If you are using hematoxylin w/ aluminum, most popular, decreased pH = decreased intensity. Acid breaks the Al+3. Check your ph throughout the process. Sounds like something has changed. Good luck. Sent from my Windows Phone ________________________________ From: Morken, Timothy via Histonet Sent: ?1/?4/?2016 9:20 AM To: Maria Mejia Cc: Histonet Subject: Re: [Histonet] Help with causes or theory behind failure of nuclear counterstaining after IHC! Maria, If the counterstain is good when done before IHC stain and poor after it sounds like proteins are being extracted during the IHC processing and staining. Have you tried staining sections after each step of the IHC process to isolate the point the stain becomes weak? Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Maria Mejia via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Sunday, January 03, 2016 8:29 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Help with causes or theory behind failure of nuclear counterstaining after IHC! Happy New Year Everyone, I'm the lead histologist working in an IHC based research lab focused on early stages of Alzheimer's disease. We work on paraffin sections processed & cut from 600um celloidin sections. Including a lot of 60um cellodin sections from whole human brainstem. For years, everything has going good regarding counterstaining after single & double IHC staining on 60um free-floating sections. However for the past two months we've struggled to achieve good visible counterstaining on IHC sections to count the stained neurons - to see clearly the nucleus & nucleolus! For a number of years, Gallocyanine was our choice of counterstain after IHC. Now, it's NOT working (neurons not stained visible enough to count). We've also tried cresyl violet counterstain - staining too weak! In both counterstains, we modified the staining protocols quite a number of times to get good visible staining - nothing!!! Strangle because we get lovely counterstained neurons with NO IHC staining on our 60um sections, but as soon as we take the sections through the IHC protocol e.g. antigen retrieval, antibodies & chromogens - counterstain is too weak! Our paraffin IHC sections work & look wonderful! Now, my PI wants to try methyl green counterstain, however I think we'll have the same problem. Here's what I need help with: 1) Can someone please explain the reason or theory behind the failure of counterstain uptake by cells such as human neurons on 60um celloidin sections? 2) Can anyone please offer staining protocols that use alternative dehydration & clearing reagents. I've been using alcohols dehydration (96% & 100%) without success as well as clearing with xylene - which hardens the tissue if left too long in this reagent. I was thinking of perhaps using acetone instead of alcohols & maybe using a methyl salicylate or chloroform. Thoughts anyone? I wish Dr Chris van der Loos was still with us. I'd dearly like to hear from anyone who can help with this issue - Gayle Callis, Terry Johnson, Dr Hohn Kiernan et al. Any assistance anyone can provide will be greatly appreciated! Best Maria Mejia UCSF Memory & Aging Department San Francisco, CA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From loree.lager at seattlechildrens.org Mon Jan 4 14:22:11 2016 From: loree.lager at seattlechildrens.org (Lager, Loree) Date: Mon, 4 Jan 2016 20:22:11 +0000 Subject: [Histonet] IHC/BAL slides Message-ID: <1B0FE3638CD0BE45A92BBA2E314B068250A3958E@PPWEXD01d.childrens.sea.kids> Hello All, Our pathologist is requesting a couple of IHC stains on air dried BAL slides, for interest. We currently only run IHC's on paraffin and frozen tissue. If anyone has protocols for CD3 and CD56, including any fixation, using the Benchmark Ultra and Ventana iView DAB detection kit, I would greatly appreciate it. Thanks, Loree L Williams Seattle Children's Hospital Histology CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From sbaldwin at mhhcc.org Mon Jan 4 14:55:07 2016 From: sbaldwin at mhhcc.org (Baldwin, Kathy) Date: Mon, 4 Jan 2016 20:55:07 +0000 Subject: [Histonet] Collodian bag for cell blocks Message-ID: Hi All Was wondering if anyone is using the Collodian bag for cell blocks. We just got it and have been using it 'works great' but all the techs are complaining of the smell.. Our ventilation has been looked at and has passed however the smell still lingers in the room. Any suggestions?? Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 Cell 812-887-3357 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From wdesalvo.cac at outlook.com Mon Jan 4 15:45:52 2016 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Mon, 4 Jan 2016 14:45:52 -0700 Subject: [Histonet] Collodian bag for cell blocks Message-ID: What method are you using? Making your own coated tubes, UCSF or MD Anderson. Are you purchasing coated tubes? Once the tube is coated, dried (using hood) and filled w dH2O, there should be no odor. Always work w/ collodion under the hood. Ethyl ether is main component. Once in cassette and processed, there should not be odor. Sent from my Windows Phone ________________________________ From: Baldwin, Kathy via Histonet Sent: ?1/?4/?2016 2:07 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Collodian bag for cell blocks Hi All Was wondering if anyone is using the Collodian bag for cell blocks. We just got it and have been using it 'works great' but all the techs are complaining of the smell.. Our ventilation has been looked at and has passed however the smell still lingers in the room. Any suggestions?? Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 Cell 812-887-3357 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Mon Jan 4 15:49:09 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 4 Jan 2016 21:49:09 +0000 (UTC) Subject: [Histonet] Collodian bag for cell blocks In-Reply-To: References: Message-ID: <1797500230.665759.1451944149476.JavaMail.yahoo@mail.yahoo.com> Do you mean "collodion"? If so it is nitrocellulose, HIGHLY flammable and even explosive. Has a characteristic "pungent" odor.Highly unsafe and it is beyond me why would you use this product. The odor can "adhere" to clothing.You may have a ventilation system that "passes inspection" but if your techs can smell it, it is there and it should not be.Have you consulted the MSDS for this chemical?I would not use it, no matter how great it works.Ren? On Monday, January 4, 2016 4:07 PM, "Baldwin, Kathy via Histonet" wrote: Hi All Was wondering if anyone is using the Collodian bag for cell blocks.? We just got it and have been using it 'works great' but all the techs are complaining of the smell.. Our ventilation has been looked at and has passed however the smell still lingers in the room.? Any suggestions?? Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana? 47546 Office 812-996-0210 Fax 812-996-0232 Cell 812-887-3357 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dw18 at uchicago.edu Mon Jan 4 15:57:57 2016 From: dw18 at uchicago.edu (David Wright) Date: Mon, 4 Jan 2016 21:57:57 +0000 Subject: [Histonet] failure of nuclear counterstaining after IHC Message-ID: Hi Histonet & Maria Is it possible that Maria really means Nissl staining of neuronal cell bodies (somata) together with their nuclei, rather than just nuclei? Tim is right to point out that the staining target is likely getting washed away during IHC processing, but I suggest it is Nissl substance getting degraded, rather than loss of proteins. Gallocyanine is a nucleic acid stain, and both cresyl violet and (m)ethyl green [are you trying to bait John Kiernan to get a reply? ;) ] are made relatively specific for nucleic acids when used in an acidic solution (sodium acetate or 1% acetic acid), at which pH only the phosphate backbones of the nucleic acids remain charged and able to bind a variety of stains. Nucleic acids include both the DNA in the nucleus as well as more widely distributed RNA. The 'Nissl substance' which reveals the neuronal cell bodies is basically the ribosomal RNA and mRNA, which nerve cell bodies have in abundance - at least while they are alive or well preserved. RNA is notoriously quickly degraded once in an aqueous environment, due to the everpresent and hard to inactivate RNases everywhere - ask any molecular biologist. I do IHC on free-floating 40um brain sections and never get good Nissl staining after incubations, although the Nissl quality on parallel sections not put through IHC is always fine. One problem with thick free-floating sections is the much longer incubation times they require for the same Ab concentration (rule of thumb: increase time with the square of the increased thickness - or half-thickness for floating sections with two accessible cut surfaces). This longer time in incubation buffer allows plenty of opportunity for RNases to go to work and destroy the Nissl substance. Note that it is the total incubation/wash time for all steps before the Nissl stain that is critical. Maria's better results after IHC with paraffin sections may simply be that these thinner sections allow much shorter Ab incubations and washes, or perhaps more of the endogenous RNases gets killed by paraffin embedding? Any way, my guess is that successful Nissl staining on the 60um sections will require much shorter incubations beforehand. With thick section IHC, that will mean jacking the Ab concentration to dizzying and expensive levels. Immunofluorescence has the advantage of much shorter total incubations, so may help. You could play with RNase inhibitors to see if that helps, but what I do is to double immunostain for a neuronal antigen (such as NeuN) as well. You have experience in double staining and these epitopes do not degrade so easily as RNA. I'd like to hear what you get to work! Best wishes - David == David A. Wright, Ph.D. University of Chicago Section of Neurosurgery, MC3026 ________________________________ ORIGINAL MESSAGES Histonet Digest, Vol 146, Issue 2 Message: 2 Date: Sun, 3 Jan 2016 20:28:58 -0800 From: Maria Mejia To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Help with causes or theory behind failure of nuclear counterstaining after IHC! Happy New Year Everyone, I'm the lead histologist working in an IHC based research lab focused on early stages of Alzheimer's disease. We work on paraffin sections processed & cut from 600um celloidin sections. Including a lot of 60um cellodin sections from whole human brainstem. For years, everything has going good regarding counterstaining after single & double IHC staining on 60um free-floating sections. However for the past two months we've struggled to achieve good visible counterstaining on IHC sections to count the stained neurons - to see clearly the nucleus & nucleolus! For a number of years, Gallocyanine was our choice of counterstain after IHC. Now, it's NOT working (neurons not stained visible enough to count). We've also tried cresyl violet counterstain - staining too weak! In both counterstains, we modified the staining protocols quite a number of times to get good visible staining - nothing!!! Strangle because we get lovely counterstained neurons with NO IHC staining on our 60um sections, but as soon as we take the sections through the IHC protocol e.g. antigen retrieval, antibodies & chromogens - counterstain is too weak! Our paraffin IHC sections work & look wonderful! Now, my PI wants to try methyl green counterstain, however I think we'll have the same problem. Here's what I need help with: 1) Can someone please explain the reason or theory behind the failure of counterstain uptake by cells such as human neurons on 60um celloidin sections? 2) Can anyone please offer staining protocols that use alternative dehydration & clearing reagents. I've been using alcohols dehydration (96% & 100%) without success as well as clearing with xylene - which hardens the tissue if left too long in this reagent. I was thinking of perhaps using acetone instead of alcohols & maybe using a methyl salicylate or chloroform. Thoughts anyone? I wish Dr Chris van der Loos was still with us. I'd dearly like to hear from anyone who can help with this issue - Gayle Callis, Terry Johnson, Dr Hohn Kiernan et al. Any assistance anyone can provide will be greatly appreciated! Best Maria Mejia UCSF Memory & Aging Department San Francisco, CA ====== Histonet Digest, Vol 146, Issue 2 Message: 4 Date: Mon, 4 Jan 2016 15:54:12 +0000 From: "Morken, Timothy" To: Maria Mejia Cc: Histonet Subject: Re: [Histonet] Help with causes or theory behind failure of nuclear counterstaining after IHC! Maria, If the counterstain is good when done before IHC stain and poor after it sounds like proteins are being extracted during the IHC processing and staining. Have you tried staining sections after each step of the IHC process to isolate the point the stain becomes weak? Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From steven.weston at utas.edu.au Mon Jan 4 17:06:27 2016 From: steven.weston at utas.edu.au (Steven Weston) Date: Mon, 4 Jan 2016 23:06:27 +0000 Subject: [Histonet] Help with causes or theory behind failure of nuclear counterstaining after IHC! (Maria Mejia) Message-ID: Maria, This may sound simplistic but I find that if I have problems such as this the first thing I do is try a new batch of the staining reagents. If you haven't had problems before then something must have changed either in your protocols or your reagents. It could be that your heat retrieval reagents are too old or contain detergents that remove some of the proteins you are looking for. Some of the proprietary heat retrieval reagents that allow you to heat retrieve without having to dewax by going through xylene have been shown to change the nuclear staining pattern and create what appear to be nuclei that are full of vacuoles. Also if the celloidion is not completely removed during your staining it may be stopping any of the higher molecular weight stains from penetrating the cells. Try leaving your sections in acetone for a number of changes to ensure full removal of the celloidion. Regards Steve Weston University of Tasmania Breathe-Well CRE Lab Manager 0408990859 University of Tasmania Electronic Communications Policy (December, 2014). This email is confidential, and is for the intended recipient only. Access, disclosure, copying, distribution, or reliance on any of it by anyone outside the intended recipient organisation is prohibited and may be a criminal offence. Please delete if obtained in error and email confirmation to the sender. The views expressed in this email are not necessarily the views of the University of Tasmania, unless clearly intended otherwise. From abadesuyi at nrh-ok.com Tue Jan 5 06:27:59 2016 From: abadesuyi at nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Tue, 5 Jan 2016 06:27:59 -0600 Subject: [Histonet] VIP Model E150/E300 Message-ID: <04EE4F75BB5FB246ADB68D69B7460443A405DDA135@MAIL.nrhnt.nrh-ok.com> Hi Does anyone has a service manual for the VIP Model E150/E300 and would like to share with me? I will greatly appreciate it. Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 abadesuyi at nrh-ok.com ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From criley at dpspa.com Tue Jan 5 07:59:49 2016 From: criley at dpspa.com (Charles Riley) Date: Tue, 5 Jan 2016 08:59:49 -0500 Subject: [Histonet] Processing of breast tissue Message-ID: We have been experiencing some issue lately with cutting our breast excisions. Recently we had two specimen where after our 8 hr run the tissue was still extremely soft. Our medical director said the fat sectioned beautifully but the fibrous tissue appeared cooked. Can anyone give a suggestion as to how we should process our breast excisions from grossing through to microtomy sectioning. Currently I have tried to get grossing to limit the specimen to 2mm by 2mm by 1mm thick. We run an 8hr process using the Thermo Shandon Excelsior processor. We cut our sections at 4um but have recently had to do them at 6um in order to get the soft samples cut. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE From LRaff at uropartners.com Tue Jan 5 11:35:05 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 5 Jan 2016 17:35:05 +0000 Subject: [Histonet] Disposal of Blocks and Slides Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B5EA84B@COLOEXCH01.uropartners.local> Our lab will be passing its 10th anniversary shortly, and for space considerations, will begin disposal of blocks and slides. None of blocks or slides from early years have patient ID, so no HIPAA concerns, but what other issues are there with disposal? What kind of waste do slides and blocks constitute? Are they considered medical waste? Any information, particularly from Illinois, appreciated. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 A celebratory post: http://www.chicagonow.com/downsize-maybe/2016/01/turning-60-along-with-some-of-my-friends/ From Diana.Martinez-Longoria at ecrmc.org Tue Jan 5 12:55:16 2016 From: Diana.Martinez-Longoria at ecrmc.org (Diana Martinez-Longoria) Date: Tue, 5 Jan 2016 18:55:16 +0000 Subject: [Histonet] Processor Validation Message-ID: <12B71261212BE94BB9FB7735484B9FA15061B11E@EXMBX01.ecrmc.ci.el-centro.ca.us> Hello Histonetters, I was wondering if any of you have processor runs for 2 and 4 hour? I am trying to run validations for our upcoming CAP inspection. The processor that we are using is Tissue Tek VIP 5 Vacuum Infiltration Processor. This will greatly help me out. Thanks in advance! Diana Martinez-Longoria Bachelors of Science in Biology Histotechnician (ASCP)cm El Centro Regional Medical Center Phone: 760-339-7267 Fax: 760-339-4570 Email:dmlongoria at ecrmc.org ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? From tbraud at holyredeemer.com Tue Jan 5 13:27:44 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 5 Jan 2016 19:27:44 +0000 Subject: [Histonet] Processing of breast tissue Message-ID: <48E053DDF6CE074DB6A7414BA05403F8055FE4@HRHEX02-HOS.holyredeemer.local> Dear Charles - If your samples are so soft that you have to turn up your microns in order to section them, then the tissue is underprocessed. Many people make the mistake of equating "underprocessed" with "under fixed". A quick way to see the level of processing is to add 50.mls of 1% Alcoholic Eosin to the 2nd 100% Alcohol on the processor. It will make no difference in later staining, but you will be able to see the level of dehydration by the level of Eosin absorbed. The Eosin will show as a red tint throughout the tissue after facing, if the dehydration is complete. If the dehydration is incomplete, you will see a red tinged ring around the outside edge of the tissue in a faced block. It there is inadequate dehydration, then you can't expect to see adequate clearing with Xylene or infiltration with paraffin, therefore - underprocessed. Our procedure for breast excision biopsies is as follows: Breast specimens are grossed in at receipt, and sectioned into cassettes. Cassettes sit for 1 day in clean formalin before processing overnight We use a Tissue-Tek VIP 5, with pressure/vacuum on each step 1 10% Neutral Buffered Formalin 37?C yes 1hr, 30min 2 10% Neutral Buffered Formalin 37?C yes 1hr, 30min 3 70% Alcohol 37?C yes 30 min 4 95% Alcohol 37?C yes 40 min 5 95% Alcohol 37?C yes 45 min 6 100% Alcohol 37?C yes 1 hr 7 100% Alcohol 37?C yes 1 hr 8 100% Alcohol 37?C yes 1 hr 9 Xylene 37?C yes 45 min 10 Xylene 37?C yes 45 min 11 Paraffin 58?C yes 1 hr 12 Paraffin 58?C yes 1 hr, 30 min 13 Paraffin 58?C yes 2 hr 14 Paraffin 58?C yes 2 hr The key factor is the quality of the gross sections. Sections must be no larger than a nickel. Certainly no thicker. The rule of thumb is that if the gross tissue touches one side of the cassette, then it must not touch the other. There must be room for solutions to circulate freely within the cassette, surrounding the tissue. Thick cut tissue is the curse of the processing. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 9. Processing of breast tissue (Charles Riley) ------------------------------ Message: 9 Date: Tue, 5 Jan 2016 08:59:49 -0500 From: Charles Riley We have been experiencing some issue lately with cutting our breast excisions. Recently we had two specimen where after our 8 hr run the tissue was still extremely soft. Our medical director said the fat sectioned beautifully but the fibrous tissue appeared cooked. Can anyone give a suggestion as to how we should process our breast excisions from grossing through to microtomy sectioning. Currently I have tried to get grossing to limit the specimen to 2mm by 2mm by 1mm thick. We run an 8hr process using the Thermo Shandon Excelsior processor. We cut our sections at 4um but have recently had to do them at 6um in order to get the soft samples cut. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE From TNMayer at mdanderson.org Tue Jan 5 15:13:06 2016 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Tue, 5 Jan 2016 21:13:06 +0000 Subject: [Histonet] Disposal of Blocks and Slides (Lester Raff MD) Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC88280AD798@D1PWPEXMBX05.mdanderson.edu> Lester, For safety's sake, as well as laymen peace of mind, I would place them in the normal tissue disposal biohazard boxes. Notify the proper areas as to what they are so that they can be aware. Most other areas in the hospital will feel better, but the number crunchers may be upset because of the number of containers being used. You cannot fill them to the top, but usually about 1/2 to 3/4 full. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 The information contained in the e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. Message: 10 Date: Tue, 5 Jan 2016 17:35:05 +0000 From: Lester Raff MD To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Disposal of Blocks and Slides Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B5EA84B at COLOEXCH01.uropartners.local> Content-Type: text/plain; charset="us-ascii" Our lab will be passing its 10th anniversary shortly, and for space considerations, will begin disposal of blocks and slides. None of blocks or slides from early years have patient ID, so no HIPAA concerns, but what other issues are there with disposal? What kind of waste do slides and blocks constitute? Are they considered medical waste? Any information, particularly from Illinois, appreciated. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 A celebratory post: http://www.chicagonow.com/downsize-maybe/2016/01/turning-60-along-with-some-of-my-friends/ ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 146, Issue 3 **************************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From flnails at texaschildrens.org Tue Jan 5 15:27:17 2016 From: flnails at texaschildrens.org (Nails, Felton) Date: Tue, 5 Jan 2016 21:27:17 +0000 Subject: [Histonet] Open Positions Message-ID: <327E034F1892504289B7A17EC71DF9F31223D562@TCFMSG03.ad.texaschildrenshospital.org> I have two open positions at Texas Childrens Hospital. There is one in the IHC lab and one in Special Stains. If interested apply at Texaschildrenshosptial.org Thanks, Felton L. Nails ______________________________________________________________________ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ______________________________________________________________________ From JMacDonald at mtsac.edu Tue Jan 5 23:34:39 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Tue, 5 Jan 2016 21:34:39 -0800 Subject: [Histonet] Processing of breast tissue In-Reply-To: References: Message-ID: Do you using "freezing spray" on these specimens? Direct spray can cause the tissue to appear burnt. From: Charles Riley via Histonet To: histonet at lists.utsouthwestern.edu Date: 01/05/2016 06:00 AM Subject: [Histonet] Processing of breast tissue We have been experiencing some issue lately with cutting our breast excisions. Recently we had two specimen where after our 8 hr run the tissue was still extremely soft. Our medical director said the fat sectioned beautifully but the fibrous tissue appeared cooked. Can anyone give a suggestion as to how we should process our breast excisions from grossing through to microtomy sectioning. Currently I have tried to get grossing to limit the specimen to 2mm by 2mm by 1mm thick. We run an 8hr process using the Thermo Shandon Excelsior processor. We cut our sections at 4um but have recently had to do them at 6um in order to get the soft samples cut. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marcus.green at oncology.ox.ac.uk Wed Jan 6 05:26:35 2016 From: marcus.green at oncology.ox.ac.uk (Marcus Green) Date: Wed, 6 Jan 2016 11:26:35 +0000 Subject: [Histonet] IHC with H & E staining Message-ID: <669331A24821FE4491665E8DFD47CF22C254A4@MBX06.ad.oak.ox.ac.uk> Dear Users, Happy New year - I hope this finds everybody well! I was asked a very simple question yesterday - why don't you do H&E counterstaining on DAB stained samples. The question came about as we're looking at CD31 staining for blood-vessels and some look ruptured (we're keen to see red blood cells). Instead of doing a consecutive cuts, a CD31 stain (Haem counterstain) on one slide and H&E on another, the question was asked why not do the Eosin stain as part of the counterstain.... I've never seen it done in the literature or in the clinic, and I've never asked why it's not done. Any assistance or advice would be greatly welcome - and my sincerest apologies if this is a very basic question? Thanks in advance for your time, kind regards, Marcus, Department of Oncology - University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford, OX3 7DQ From liz at premierlab.com Wed Jan 6 11:20:30 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Wed, 6 Jan 2016 10:20:30 -0700 Subject: [Histonet] IHC with H & E staining In-Reply-To: <669331A24821FE4491665E8DFD47CF22C254A4@MBX06.ad.oak.ox.ac.uk> References: <669331A24821FE4491665E8DFD47CF22C254A4@MBX06.ad.oak.ox.ac.uk> Message-ID: <14E2C6176416974295479C64A11CB9AE02852917C873@SBS2K8.premierlab.local> Marcus You can stain with H&E after DAB if you think it would help your how you analyze your slides. DAB with the addition of a special stain has been published and for us depending upon the project that we are working on we may choose to use a different counterstain after an IHC that has DAB as an chromogen. For example we have worked with macrophage markers counterstained with Prussian blue, another example is brain IHC markers counterstained Cresyl ect Violet rather than hematoxylin. DAB is extremely stable and therefore many special stains or different counterstains can be used. If you are running an image analysis algorithm most canned algorithms are set up to function with a hematoxylin counterstain - you would need evaluate if a different counterstain would decrease the accuracy of your algorithm but you may have the ability to design an algorithm with the counterstain of your choice. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax liz at premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: Marcus Green via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, January 06, 2016 4:26 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC with H & E staining Dear Users, Happy New year - I hope this finds everybody well! I was asked a very simple question yesterday - why don't you do H&E counterstaining on DAB stained samples. The question came about as we're looking at CD31 staining for blood-vessels and some look ruptured (we're keen to see red blood cells). Instead of doing a consecutive cuts, a CD31 stain (Haem counterstain) on one slide and H&E on another, the question was asked why not do the Eosin stain as part of the counterstain.... I've never seen it done in the literature or in the clinic, and I've never asked why it's not done. Any assistance or advice would be greatly welcome - and my sincerest apologies if this is a very basic question? Thanks in advance for your time, kind regards, Marcus, Department of Oncology - University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford, OX3 7DQ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz at premierlab.com Wed Jan 6 13:23:34 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Wed, 6 Jan 2016 12:23:34 -0700 Subject: [Histonet] VIP Model E150/E300 In-Reply-To: <04EE4F75BB5FB246ADB68D69B7460443A405DDA135@MAIL.nrhnt.nrh-ok.com> References: <04EE4F75BB5FB246ADB68D69B7460443A405DDA135@MAIL.nrhnt.nrh-ok.com> Message-ID: <14E2C6176416974295479C64A11CB9AE028529166B23@SBS2K8.premierlab.local> I have an operator/user manual that I can pdf for you but I do not have the service manual. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Adesupo, Adesuyi (Banjo) via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, January 05, 2016 5:28 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] VIP Model E150/E300 Hi Does anyone has a service manual for the VIP Model E150/E300 and would like to share with me? I will greatly appreciate it. Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 abadesuyi at nrh-ok.com ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dw18 at uchicago.edu Wed Jan 6 15:15:49 2016 From: dw18 at uchicago.edu (David Wright) Date: Wed, 6 Jan 2016 21:15:49 +0000 Subject: [Histonet] DAB IHC with H & E staining Message-ID: Hi Histonet, Marcus and Liz I agree with everything Liz says about precipitated DAB being wonderfully robust so that you can do a whole range of stains successfully after using it. I am slightly puzzled that Marcus wants to reveal RBCs after a DAB reaction. Doesn't it just happen any way? Haem/hemoglobin provides a powerful non-specific peroxidase (the frothing you get with peroxide on dried blood) which will also precipitate DAB in the RBCs - I see it whenever I don't fully perfuse my brains. If you're not seeing this currently, and you are sure there are RBCs present, it might simply suffice to ease up on whatever procedure you use to "kill' endogenous peroxidases before your Ab incubations, so that the haem can go to work on the DAB. Note that if you happen to use thick sections for floating immuno, you really don't want to use eosin. There is so much material that everything is red. (There is a similar 'too much' problem with using solochrome cyanine on thick sections for myelin...) best to all - David == David A. Wright, Ph.D. University of Chicago Section of Neurosurgery, MC3026 ________________________________ ORIGINAL MESSAGES ------------------------------ Histonet Digest, Vol 146, Issue 4 Message: 7 Date: Wed, 6 Jan 2016 10:20:30 -0700 From: Elizabeth Chlipala To: Marcus Green , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] IHC with H & E staining Marcus You can stain with H&E after DAB if you think it would help your how you analyze your slides. DAB with the addition of a special stain has been published and for us depending upon the project that we are working on we may choose to use a different counterstain after an IHC that has DAB as an chromogen. For example we have worked with macrophage markers counterstained with Prussian blue, another example is brain IHC markers counterstained Cresyl ect Violet rather than hematoxylin. DAB is extremely stable and therefore many special stains or different counterstains can be used. If you are running an image analysis algorithm most canned algorithms are set up to function with a hematoxylin counterstain - you would need evaluate if a different counterstain would decrease the accuracy of your algorithm but you may have the ability to design an algorithm with the counterstain of your choice. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC liz at premierlab.com ________________________________________ From: Marcus Green via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, January 06, 2016 4:26 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC with H & E staining Dear Users, Happy New year - I hope this finds everybody well! I was asked a very simple question yesterday - why don't you do H&E counterstaining on DAB stained samples. The question came about as we're looking at CD31 staining for blood-vessels and some look ruptured (we're keen to see red blood cells). Instead of doing a consecutive cuts, a CD31 stain (Haem counterstain) on one slide and H&E on another, the question was asked why not do the Eosin stain as part of the counterstain.... I've never seen it done in the literature or in the clinic, and I've never asked why it's not done. Any assistance or advice would be greatly welcome - and my sincerest apologies if this is a very basic question? Thanks in advance for your time, kind regards, Marcus, Department of Oncology - University of Oxford, From liz at premierlab.com Wed Jan 6 15:39:57 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Wed, 6 Jan 2016 14:39:57 -0700 Subject: [Histonet] DAB IHC with H & E staining In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE028529166B2D@SBS2K8.premierlab.local> That's correct if you do not quench endogenous peroxidase activity completely you would be able to visualize the RBC's but maybe in instances like this it might be better to have the rbc's stain a different color than the DAB they would be easier to recognize, especially if they are specifically looking at neovascularization and subtle changes in the vasculature. DAB can be great but it also can pose problems due to endogenous pigments that appear light brown or brown in color. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: David Wright [mailto:dw18 at uchicago.edu] Sent: Wednesday, January 06, 2016 2:16 PM To: histonet at lists.utsouthwestern.edu Cc: Elizabeth Chlipala Subject: RE: DAB IHC with H & E staining Hi Histonet, Marcus and Liz I agree with everything Liz says about precipitated DAB being wonderfully robust so that you can do a whole range of stains successfully after using it. I am slightly puzzled that Marcus wants to reveal RBCs after a DAB reaction. Doesn't it just happen any way? Haem/hemoglobin provides a powerful non-specific peroxidase (the frothing you get with peroxide on dried blood) which will also precipitate DAB in the RBCs - I see it whenever I don't fully perfuse my brains. If you're not seeing this currently, and you are sure there are RBCs present, it might simply suffice to ease up on whatever procedure you use to "kill' endogenous peroxidases before your Ab incubations, so that the haem can go to work on the DAB. Note that if you happen to use thick sections for floating immuno, you really don't want to use eosin. There is so much material that everything is red. (There is a similar 'too much' problem with using solochrome cyanine on thick sections for myelin...) best to all - David == David A. Wright, Ph.D. University of Chicago Section of Neurosurgery, MC3026 ________________________________ ORIGINAL MESSAGES ------------------------------ Histonet Digest, Vol 146, Issue 4 Message: 7 Date: Wed, 6 Jan 2016 10:20:30 -0700 From: Elizabeth Chlipala > To: Marcus Green >, "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] IHC with H & E staining Marcus You can stain with H&E after DAB if you think it would help your how you analyze your slides. DAB with the addition of a special stain has been published and for us depending upon the project that we are working on we may choose to use a different counterstain after an IHC that has DAB as an chromogen. For example we have worked with macrophage markers counterstained with Prussian blue, another example is brain IHC markers counterstained Cresyl ect Violet rather than hematoxylin. DAB is extremely stable and therefore many special stains or different counterstains can be used. If you are running an image analysis algorithm most canned algorithms are set up to function with a hematoxylin counterstain - you would need evaluate if a different counterstain would decrease the accuracy of your algorithm but you may have the ability to design an algorithm with the counterstain of your choice. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC liz at premierlab.com ________________________________________ From: Marcus Green via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, January 06, 2016 4:26 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC with H & E staining Dear Users, Happy New year - I hope this finds everybody well! I was asked a very simple question yesterday - why don't you do H&E counterstaining on DAB stained samples. The question came about as we're looking at CD31 staining for blood-vessels and some look ruptured (we're keen to see red blood cells). Instead of doing a consecutive cuts, a CD31 stain (Haem counterstain) on one slide and H&E on another, the question was asked why not do the Eosin stain as part of the counterstain.... I've never seen it done in the literature or in the clinic, and I've never asked why it's not done. Any assistance or advice would be greatly welcome - and my sincerest apologies if this is a very basic question? Thanks in advance for your time, kind regards, Marcus, Department of Oncology - University of Oxford, From tawnia at ampianstaffing.com Wed Jan 6 16:32:55 2016 From: tawnia at ampianstaffing.com (Tawnia Lindsay) Date: Wed, 6 Jan 2016 22:32:55 +0000 Subject: [Histonet] Hot Histology Jobs In-Reply-To: References: Message-ID: Happy New year Histonetters!!! I have a couple of amazing travel or travel to perm opportunities throughout the US. I need 5-10 great people to start in the next 2- 4 weeks. I have 3 positions in Missouri, 2 in Washington state, 1 in California, and 1 in Nevada. If you are currently looking for a great new travel opportunity please give me a call at 877-229-6996 ext 2009 or email me at tawnia at ampianstaffing.com Thanks! *************************** From camgomes10 at gmail.com Thu Jan 7 08:47:32 2016 From: camgomes10 at gmail.com (Camila Gomes) Date: Thu, 7 Jan 2016 14:47:32 +0000 Subject: [Histonet] CD31 Antibody Message-ID: Dear all, I am planning an experiment for staining (immunofluorescence) human sections with 3 different antibodies: -CD31/Laminin 5 -CD31/Pan-laminin Laminin 5 is from mouse and pan laminin is from rabbit and I would like to know if someone can advise me a good CD31 antibody from a different specie or 2 CD31 antibodies (same clone) to use with the laminin antibodies. I am struggling to find a good combination to go ahead with the experiment. Thank you in advance for your time. Kind Regards, Camila Gomes From md at personifysearch.com Thu Jan 7 09:12:35 2016 From: md at personifysearch.com (Mary Southgate Dickson) Date: Thu, 7 Jan 2016 10:12:35 -0500 Subject: [Histonet] Field Histology Specialist Opportunity- IHC Message-ID: Hello Histonet! We are currently recruiting for a field based histology (IHC) technical specialist role with a world leading cancer diagnostics company. We are currently targeting IHC professionals in the Northwest (ideally Seattle or Portland). This is a field based role and offers a very compentitve package including: base salary, bonus, paid vacation, company car, laptop, cell phone, and exceptional benefits. If you are interested in learning more, please contact me directly at md at personifysearch.com Thanks! Mary Southgate Mary Southgate Dickson Talent Management Executive Personify 401 Harrison Oaks Boulevard Suite 350 Cary, North Carolina 27513 www.personifysearch.com 800.875.6188 x 130 http://www.linkedin.com/in/marysouthgatedickson From wdesalvo.cac at outlook.com Thu Jan 7 15:31:44 2016 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Thu, 7 Jan 2016 14:31:44 -0700 Subject: [Histonet] NSH Financial and Tax Statements Message-ID: As a due paying member of NSH, I ask all members and anyone interested in Histotechnology request from NSH office to see the Financial and Tax Statements for 2013 / 2014. This is public information. We should all know where our dues money, money from educational activities and vendor money from the convention is being spent. Sent from my Windows Phone From steven.weston at utas.edu.au Thu Jan 7 16:46:59 2016 From: steven.weston at utas.edu.au (Steven Weston) Date: Thu, 7 Jan 2016 22:46:59 +0000 Subject: [Histonet] H and E Staining in IHC Message-ID: Hi Marcus, As stated previously by others there should be no problem putting the Eosin into your sections but I would suggest that you do not leave out the endogenous quenching so you can show your red blood cells. The reason being that there are many other cells that may then show up as positive. Instead I suggest using an Eosin/Phloxine mixture which nicely highlights the red cells differently to the rest of the general matrix material. regards Steve Weston Breathe-Well CRE Lab Manager 0408990859 University of Tasmania Electronic Communications Policy (December, 2014). This email is confidential, and is for the intended recipient only. Access, disclosure, copying, distribution, or reliance on any of it by anyone outside the intended recipient organisation is prohibited and may be a criminal offence. Please delete if obtained in error and email confirmation to the sender. The views expressed in this email are not necessarily the views of the University of Tasmania, unless clearly intended otherwise. From wbenton at cua.md Thu Jan 7 16:59:00 2016 From: wbenton at cua.md (Walter Benton) Date: Thu, 7 Jan 2016 22:59:00 +0000 Subject: [Histonet] NSH Financial and Tax Statements In-Reply-To: References: Message-ID: <1452207540684.14062@cua.md> The website has some of the information you are speaking about, but I don't think tax statements are usually provided. They have the financials there for your review and I have included the link as well. http://www.nsh.org/sites/default/files/2014%20Annual%20Report%20Final.pdf Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: WILLIAM DESALVO via Histonet Sent: Thursday, January 7, 2016 4:31 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] NSH Financial and Tax Statements As a due paying member of NSH, I ask all members and anyone interested in Histotechnology request from NSH office to see the Financial and Tax Statements for 2013 / 2014. This is public information. We should all know where our dues money, money from educational activities and vendor money from the convention is being spent. Sent from my Windows Phone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From amosbrooks at gmail.com Thu Jan 7 17:03:41 2016 From: amosbrooks at gmail.com (Amos Brooks) Date: Thu, 7 Jan 2016 18:03:41 -0500 Subject: [Histonet] IHC with H & E staining Message-ID: Hi, I usually try to avoid eosin as a counterstain for a DAB labeled slide because the red/pink of the eosin can obscure the rusty brown of DAB. If you really want to use it though I would suggest a *really* light eosin, perhaps even just a few milliliters in the 95% ETOH as you are dehydrating it. The RBCs and eosinophils will pick it up quickly and the stain should not overwhelm the DAB. You could also darken the DAB with Copper sulfate. Cheers, Amos On Wed, Jan 6, 2016 at 1:00 PM, wrote: > Message: 6 > Date: Wed, 6 Jan 2016 11:26:35 +0000 > From: Marcus Green > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] IHC with H & E staining > Message-ID: > <669331A24821FE4491665E8DFD47CF22C254A4 at MBX06.ad.oak.ox.ac.uk> > Content-Type: text/plain; charset="iso-8859-1" > > Dear Users, > > > > Happy New year - I hope this finds everybody well! > > > > I was asked a very simple question yesterday - why don't you do H&E > counterstaining on DAB stained samples. The question came about as we're > looking at CD31 staining for blood-vessels and some look ruptured (we're > keen to see red blood cells). > > > > Instead of doing a consecutive cuts, a CD31 stain (Haem counterstain) on > one slide and H&E on another, the question was asked why not do the Eosin > stain as part of the counterstain.... > > > > I've never seen it done in the literature or in the clinic, and I've never > asked why it's not done. Any assistance or advice would be greatly welcome > - and my sincerest apologies if this is a very basic question? > > > > Thanks in advance for your time, > > kind regards, > > > > Marcus, > From clmcmah at clemson.edu Thu Jan 7 19:18:48 2016 From: clmcmah at clemson.edu (Chad L McMahan) Date: Fri, 8 Jan 2016 01:18:48 +0000 Subject: [Histonet] NSH Financial and Tax Statements In-Reply-To: <1452207540684.14062@cua.md> References: , <1452207540684.14062@cua.md> Message-ID: <48214F1F-6EB8-46A9-94CD-B2E7E0810C0D@clemson.edu> Chad McMahan, NSH member, Region III, SC President, I agree with William DeSalvo. We need to see valid tax records and not the readily formatted version of the Presidents report. I would hate for the consensus of membership to be easily swayed to believe that tax records to the IRS and accounting does not show the same validity as the 2014 report. I agree with another colleague that vendors or anyone associated with a for profit organization should not hold BOD positions according to the original bylaws. We should change that this year and not look back! What about Region III candidates to fill current position. We as members want to do what is right for the whole organization. We would love for our voices to be heard and not challenged. I would like to see complete organization from the top down. If it means walking away from political status and saying publicly and apology then do it. I have seen over the past year some very influential walk away from NSH. I have seen people stay that need to go! Let's consider taking it back to the basics without legalese jargon and worrying if your colleague is going to sue you or not. When I look back at the 25th Anniversary Calendar of NSH I done see what we have now. It's a true celebrated event over 25 years ago. What happened! I look at the leadership current! What would Lee Luna think of what we have and insure that the legacy continued? We would love to see the validity of the expenses please as supporting members. We have the authority to seek and have answers met. Best regards, Chad M Sent from my iPhone > On Jan 7, 2016, at 6:00 PM, Walter Benton via Histonet wrote: > > The website has some of the information you are speaking about, but I don't think tax statements are usually provided. They have the financials there for your review and I have included the link as well. > > http://www.nsh.org/sites/default/files/2014%20Annual%20Report%20Final.pdf > > > Walter Benton HT(ASCP)QIHC > Lab Operations Manager > Chesapeake Urology Associates > 806 Landmark Drive, Suite 127 > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > Chesapeakeurology.com > > Voted a Best Place to Work by > Baltimore and Modern Healthcare > Magazines. > ________________________________________ > From: WILLIAM DESALVO via Histonet > Sent: Thursday, January 7, 2016 4:31 PM > To: 'histonet at lists.utsouthwestern.edu' > Subject: [Histonet] NSH Financial and Tax Statements > > As a due paying member of NSH, I ask all members and anyone interested in Histotechnology request from NSH office to see the Financial and Tax Statements for 2013 / 2014. This is public information. We should all know where our dues money, money from educational activities and vendor money from the convention is being spent. > > Sent from my Windows Phone > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SteveM at mcclainlab.com Fri Jan 8 05:56:55 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Fri, 8 Jan 2016 11:56:55 +0000 Subject: [Histonet] Histonet Digest, Vol 146, Issue 4 validation suggestions for short cycle processing with conventional equipment In-Reply-To: References: Message-ID: <5D4461F7-661B-4B33-9F3E-27D7679BF033@mcclainlab.com> I posted our 7 year experience on this topic previously on histonet. Remember, this machine (K3000, VIP5) was not designed specifically for this purpose, but it can be made to work admirably. IOW Short processing on VIP is unreliable UNLESS you are willing to accept or control certain conditions, 1) only process well-fixed tissue 2) take formalin off this processor, making 14 changes beginning in 50%. If you want to keep station 1 formalin and standardize fixation with time X in formalin at temp Y, that may work. I prefer to change the tissues to 50% alcohol at the grossing bench so that all tissues are not only fixed, but rinsed in alcohol and started processing at the growing bench. This simplifies processing to dehydration, clearing and paraffin infiltration. Simple is reliable and short processing cycles on this machine leave little room for error. 2) Program heat to 50C in all alcohols and xylene. Mixing pressure and vacuum yes yes yes 4) Keep all solutions fresh, rotate after 200- 400 blocks. 5) Lay blocks out not more than 2 cassettes back to back, max 100 for VIP5. 4 hour is reliable for tissues up to 3mm thickness such as punch biopsy. 2 hour is reliable for up to 1.5 mm tissue thickness such as core biopsies and shave biopsies of skin. Method of processor validation for short cycle in machine previously validated in your lab. To validate create your evaluation form and find a set of cases with sufficient tissue small tissue to divide into 2 equal blocks. This can (and will) be done on clinical cases if you are careful. Print duplicate sets of cassettes. Mark one half of case 1 yellow and one half blue (tissues will be processed separately, 1 regular and one short cycle and then recombined into 1 final block at embedding). We held the short processed tissue cassettes in formalin and timed the short cycle to end when the regular program ends. Then the two blocks are recombined or embedded together so that direct comparisons/measures can be made on one H&E and one on your most common IHC for that tissue. If the H&E and IHC both work, chances are everything else will also. Modify your regular validation form or Create a form for scoring shrinkage, measured thickness and length, staining reactions, shrinkage, holes and flaws, have your path score. photo graph yellow and blue tissues under H&E and IHC. IF you do observe shrinkage or other undesirable artifact, be certain that your tissues are completely fixed at grossing and repeat. If they are comparable in the oculars and to the camera, then show your work and voila, there is your validation. Steve A. McClain, MD > From jqb7 at cdc.gov Fri Jan 8 07:26:53 2016 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Fri, 8 Jan 2016 13:26:53 +0000 Subject: [Histonet] lab humidifiers Message-ID: <3B2CD438E1628A41BD687E98B963B7813A5A9DF6@EMBX-CLFT4.cdc.gov> Morning everyone! What is the standard in humidity levels that most histology labs strive for? And to achieve this goal, do any of you use a commercial-grade humidifier? Thanks for your help and have a nice weekend! Jeanine H. Sanders Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS-G32 Atlanta, GA 30329 From LRaff at uropartners.com Fri Jan 8 07:49:18 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 8 Jan 2016 13:49:18 +0000 Subject: [Histonet] Thanks for assistance Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B5F2347@COLOEXCH01.uropartners.local> Thanks for spreading the word about block disposal. I have been inundated with requests, will have to sort through them all! For anyone interested Friday blog post http://www.chicagonow.com/downsize-maybe/2016/01/what-is-the-last-sound-you-will-hear/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From histology81176 at att.net Fri Jan 8 08:42:33 2016 From: histology81176 at att.net (Histology Technician) Date: Fri, 8 Jan 2016 14:42:33 +0000 (UTC) Subject: [Histonet] Price gun References: <447350760.1678249.1452264153035.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <447350760.1678249.1452264153035.JavaMail.yahoo@mail.yahoo.com> ? I was thinking of using a price gun to date my supplies when they come in, but I can't find one to buy.? Any ideas where to search for one?What do you guys use to date your supplies?? I've always used a pen to write the rcd date and my initials but thought a price gun would be quicker and neater :) Thanks! From mjones at metropath.com Fri Jan 8 09:01:08 2016 From: mjones at metropath.com (Michael Ann Jones) Date: Fri, 8 Jan 2016 15:01:08 +0000 Subject: [Histonet] lab humidifiers Message-ID: We are struggling with our humidity constantly. Colorado is dry in the winter. We use at least two humidifiers to try to keep humidity at minimum 30% due to equipment manufacturer?s specs. We have log sheets and thermometer/humidifier measurers to keep track of humidity. Standard for equipment seems to be 30 - 80% humidity per manufacturer?s specifications of processors, etc. Good luck Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com Providing collaborative diagnostic services, saving lives today and tomorrow. On 1/8/16, 6:26 AM, "Sanders, Jeanine (CDC/OID/NCEZID) via Histonet" wrote: >Morning everyone! > >What is the standard in humidity levels that most histology labs strive >for? And to achieve this goal, do any of you use a commercial-grade >humidifier? > >Thanks for your help and have a nice weekend! > > >Jeanine H. Sanders >Centers for Disease Control and Prevention >1600 Clifton Rd., NE MS-G32 >Atlanta, GA 30329 > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ENarvaez at Pathlinelabs.com Fri Jan 8 09:35:17 2016 From: ENarvaez at Pathlinelabs.com (Edison Narvaez) Date: Fri, 8 Jan 2016 15:35:17 +0000 Subject: [Histonet] HPV 16/18 background Message-ID: <10d75e4b1c4f450ca1a4686118969218@RAMEXCHANGE.HPS.LOCAL> Hello, I'm running HPV probes from Enzo on my Leica instruments, and we are noticing background episomal pattern on 16/18 staining of cases that are strongly positive for HPV 6/11, but not on cases that are 6/11 or 16/18 negative. Is anybody experiencing this same issue and are there any suggestions. I'm using Enzo probes, Enzo Stringency wash, Enzo mouse-antibiotin, and a polymer detection kit. Thank you ________________________________ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. From relia1 at earthlink.net Fri Jan 8 09:31:34 2016 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 8 Jan 2016 10:31:34 -0500 Subject: [Histonet] Happy New Year! Here's to a Fantastic 2016! Message-ID: <00b601d14a29$a87eeca0$f97cc5e0$@earthlink.net> Hi Histonetters! Happy New Year!!!! Here's to a terrific 2016!!!!!!! How did you ring in the New Year? I hope you had fun ushering out 2015 and welcoming in 2016. I know I am looking forward to an exciting new year. I wanted to drop you this quick line to let you know that I have been chatting with clients and have some exciting new opportunities. Here is a list of my current open positions: Leadership: Histology Lab Manager - Modesto, CA Histology Supervisor (Dermpath) - Fayetteville, AR Senior Histotech - Norfolk, VA B.S. required Histotechnicians/Histotechnologists: Histology Tech - Norfolk, VA 15K Sign on Bonus Histology Tech - Modesto, CA Mohs Tech - Naples, FL HT/HTL - Milwaukee, WI Dermpath Histotech - Kansas City, KS Histotech - Louisville, KY (learn electron microscopy) Dermpath Histotech - Tyler, TX Histotech - San Diego, CA Tech Support - Los Angeles, CA All of these are full time positions and my clients offer excellent compensation, benefits and in most cases relocation assistance and or a sign on bonus. For more information please contact me by e-mail: relia1 at earthlink.net or toll free: 866-607-3542 or you can reach me on my cell/text: 407-353-5070. If none of these positions interest you and you are on the hunt for a new position NOW please drop me an email and let me know what you are looking for. My phone is ringing off the hook and your next opportunity could be just a phone call away. When I get the call I want to know to call YOU! If you are set in your current position Congratulations! and thank you for taking the time to read my email. I have new positions coming in all of the time and you never know when something might interest you or a friend or coworker. The best part is if you refer someone and I place them you will earn a referral fee! So please stay tuned for my next email! Happy New Year to You & Yours!! I wish you a year of love, laughter, good health & prosperity. Thanks-Pam Happy New Year! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From jqb7 at cdc.gov Fri Jan 8 09:32:04 2016 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Fri, 8 Jan 2016 15:32:04 +0000 Subject: [Histonet] lab humidifiers In-Reply-To: References: Message-ID: <3B2CD438E1628A41BD687E98B963B7813A5A9F3A@EMBX-CLFT4.cdc.gov> Thanks. We dog log the temp. I have had several service techs come in and comment on how much static we have. It has been worse than usual lately so am looking into options. Thanks much for your input! ________________________________________ From: Michael Ann Jones [mjones at metropath.com] Sent: Friday, January 08, 2016 10:01 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] lab humidifiers We are struggling with our humidity constantly. Colorado is dry in the winter. We use at least two humidifiers to try to keep humidity at minimum 30% due to equipment manufacturer?s specs. We have log sheets and thermometer/humidifier measurers to keep track of humidity. Standard for equipment seems to be 30 - 80% humidity per manufacturer?s specifications of processors, etc. Good luck Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com Providing collaborative diagnostic services, saving lives today and tomorrow. On 1/8/16, 6:26 AM, "Sanders, Jeanine (CDC/OID/NCEZID) via Histonet" wrote: >Morning everyone! > >What is the standard in humidity levels that most histology labs strive >for? And to achieve this goal, do any of you use a commercial-grade >humidifier? > >Thanks for your help and have a nice weekend! > > >Jeanine H. Sanders >Centers for Disease Control and Prevention >1600 Clifton Rd., NE MS-G32 >Atlanta, GA 30329 > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From caithesketh at gmail.com Fri Jan 8 12:09:06 2016 From: caithesketh at gmail.com (Caitlin Hesketh) Date: Fri, 8 Jan 2016 13:09:06 -0500 Subject: [Histonet] job in Boston area Message-ID: Hi everyone! I'm currently looking for a Histotech - two shifts available, both early morning, 8 hour, Tues-Saturday. Primarily someone to embed and cut, opportunities to do specials and IHC staining as well. We're a small but busy lab just outside Boston that does exclusively derm specimens. If you're interested or know anyone who might be please email caithesketh at gmail.com. Thank you! From mjdessoye at commonwealthhealth.net Fri Jan 8 12:27:58 2016 From: mjdessoye at commonwealthhealth.net (Dessoye, Michael) Date: Fri, 8 Jan 2016 18:27:58 +0000 Subject: [Histonet] Price gun In-Reply-To: <447350760.1678249.1452264153035.JavaMail.yahoo@mail.yahoo.com> References: <447350760.1678249.1452264153035.JavaMail.yahoo.ref@mail.yahoo.com> <447350760.1678249.1452264153035.JavaMail.yahoo@mail.yahoo.com> Message-ID: Check this out: http://www.staples.com/Garvey-reg-1-Line-Pricemarker/product_69516 Lots of types available at Staples, Grainger, etc. It will save you a lot of writing. Michael J. Dessoye, M.S.?|?Histology/Toxicology/RIA Supervisor?|?Wilkes-Barre General Hospital?|?An Affiliate of Commonwealth Health |?mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 -----Original Message----- From: Histology Technician [mailto:histology81176 at att.net] Sent: Friday, January 08, 2016 9:43 AM To: Histonet List; histonet-request at lists.utsouthwestern.edu Subject: [Histonet] Price gun ? I was thinking of using a price gun to date my supplies when they come in, but I can't find one to buy.? Any ideas where to search for one?What do you guys use to date your supplies?? I've always used a pen to write the rcd date and my initials but thought a price gun would be quicker and neater :) Thanks! -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From badams at acadianagastro.com Fri Jan 8 12:57:54 2016 From: badams at acadianagastro.com (Brent Adams) Date: Fri, 8 Jan 2016 18:57:54 +0000 Subject: [Histonet] Price Gun In-Reply-To: References: Message-ID: I ordered mine on ULINE.com and got it for $128.00 they also supply the sticker rolls. This helped me as I get standing orders once a month. I apply the received sticker next to the containers exp: date. When I open the container and only use a portion. I circle the EXP: Date, and Received date sticker in Red and hand write the date it was opened. This has expedited being able to put supplies away and looks a lot cleaner during inspections. good luck Brent Adams ? BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Friday, January 8, 2016 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 146, Issue 6 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. NSH Financial and Tax Statements (WILLIAM DESALVO) 2. H and E Staining in IHC (Steven Weston) 3. Re: NSH Financial and Tax Statements (Walter Benton) 4. Re: IHC with H & E staining (Amos Brooks) 5. Re: NSH Financial and Tax Statements (Chad L McMahan) 6. Re: Histonet Digest, Vol 146, Issue 4 validation suggestions for short cycle processing with conventional equipment (Steve McClain) 7. lab humidifiers (Sanders, Jeanine (CDC/OID/NCEZID)) 8. Thanks for assistance (Lester Raff MD) 9. Price gun (Histology Technician) 10. Re: lab humidifiers (Michael Ann Jones) 11. HPV 16/18 background (Edison Narvaez) 12. Happy New Year! Here's to a Fantastic 2016! (Pam Barker) 13. Re: lab humidifiers (Sanders, Jeanine (CDC/OID/NCEZID)) ---------------------------------------------------------------------- Message: 1 Date: Thu, 7 Jan 2016 14:31:44 -0700 From: WILLIAM DESALVO To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] NSH Financial and Tax Statements Message-ID: Content-Type: text/plain; charset="Windows-1252" As a due paying member of NSH, I ask all members and anyone interested in Histotechnology request from NSH office to see the Financial and Tax Statements for 2013 / 2014. This is public information. We should all know where our dues money, money from educational activities and vendor money from the convention is being spent. Sent from my Windows Phone ------------------------------ Message: 2 Date: Thu, 7 Jan 2016 22:46:59 +0000 From: Steven Weston To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] H and E Staining in IHC Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Marcus, As stated previously by others there should be no problem putting the Eosin into your sections but I would suggest that you do not leave out the endogenous quenching so you can show your red blood cells. The reason being that there are many other cells that may then show up as positive. Instead I suggest using an Eosin/Phloxine mixture which nicely highlights the red cells differently to the rest of the general matrix material. regards Steve Weston Breathe-Well CRE Lab Manager 0408990859 University of Tasmania Electronic Communications Policy (December, 2014). This email is confidential, and is for the intended recipient only. Access, disclosure, copying, distribution, or reliance on any of it by anyone outside the intended recipient organisation is prohibited and may be a criminal offence. Please delete if obtained in error and email confirmation to the sender. The views expressed in this email are not necessarily the views of the University of Tasmania, unless clearly intended otherwise. ------------------------------ Message: 3 Date: Thu, 7 Jan 2016 22:59:00 +0000 From: Walter Benton To: WILLIAM DESALVO , Histonet Subject: Re: [Histonet] NSH Financial and Tax Statements Message-ID: <1452207540684.14062 at cua.md> Content-Type: text/plain; charset="iso-8859-1" The website has some of the information you are speaking about, but I don't think tax statements are usually provided. They have the financials there for your review and I have included the link as well. http://www.nsh.org/sites/default/files/2014%20Annual%20Report%20Final.pdf Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. ________________________________________ From: WILLIAM DESALVO via Histonet Sent: Thursday, January 7, 2016 4:31 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] NSH Financial and Tax Statements As a due paying member of NSH, I ask all members and anyone interested in Histotechnology request from NSH office to see the Financial and Tax Statements for 2013 / 2014. This is public information. We should all know where our dues money, money from educational activities and vendor money from the convention is being spent. Sent from my Windows Phone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ------------------------------ Message: 4 Date: Thu, 7 Jan 2016 18:03:41 -0500 From: Amos Brooks To: "histonet at lists.utsouthwestern.edu" , marcus.green at oncology.ox.ac.uk Subject: Re: [Histonet] IHC with H & E staining Message-ID: Content-Type: text/plain; charset=UTF-8 Hi, I usually try to avoid eosin as a counterstain for a DAB labeled slide because the red/pink of the eosin can obscure the rusty brown of DAB. If you really want to use it though I would suggest a *really* light eosin, perhaps even just a few milliliters in the 95% ETOH as you are dehydrating it. The RBCs and eosinophils will pick it up quickly and the stain should not overwhelm the DAB. You could also darken the DAB with Copper sulfate. Cheers, Amos On Wed, Jan 6, 2016 at 1:00 PM, wrote: > Message: 6 > Date: Wed, 6 Jan 2016 11:26:35 +0000 > From: Marcus Green > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] IHC with H & E staining > Message-ID: > <669331A24821FE4491665E8DFD47CF22C254A4 at MBX06.ad.oak.ox.ac.uk> > Content-Type: text/plain; charset="iso-8859-1" > > Dear Users, > > > > Happy New year - I hope this finds everybody well! > > > > I was asked a very simple question yesterday - why don't you do H&E > counterstaining on DAB stained samples. The question came about as we're > looking at CD31 staining for blood-vessels and some look ruptured (we're > keen to see red blood cells). > > > > Instead of doing a consecutive cuts, a CD31 stain (Haem counterstain) on > one slide and H&E on another, the question was asked why not do the Eosin > stain as part of the counterstain.... > > > > I've never seen it done in the literature or in the clinic, and I've never > asked why it's not done. Any assistance or advice would be greatly welcome > - and my sincerest apologies if this is a very basic question? > > > > Thanks in advance for your time, > > kind regards, > > > > Marcus, > ------------------------------ Message: 5 Date: Fri, 8 Jan 2016 01:18:48 +0000 From: Chad L McMahan To: Walter Benton Cc: WILLIAM DESALVO , Histonet Subject: Re: [Histonet] NSH Financial and Tax Statements Message-ID: <48214F1F-6EB8-46A9-94CD-B2E7E0810C0D at clemson.edu> Content-Type: text/plain; charset="us-ascii" Chad McMahan, NSH member, Region III, SC President, I agree with William DeSalvo. We need to see valid tax records and not the readily formatted version of the Presidents report. I would hate for the consensus of membership to be easily swayed to believe that tax records to the IRS and accounting does not show the same validity as the 2014 report. I agree with another colleague that vendors or anyone associated with a for profit organization should not hold BOD positions according to the original bylaws. We should change that this year and not look back! What about Region III candidates to fill current position. We as members want to do what is right for the whole organization. We would love for our voices to be heard and not challenged. I would like to see complete organization from the top down. If it means walking away from political status and saying publicly and apology then do it. I have seen over the past year some very influential walk away from NSH. I have seen people stay that need to go! Let's consider taking it back to the basics without legalese jargon and worrying if your colleague is going to sue you or not. When I look back at the 25th Anniversary Calendar of NSH I done see what we have now. It's a true celebrated event over 25 years ago. What happened! I look at the leadership current! What would Lee Luna think of what we have and insure that the legacy continued? We would love to see the validity of the expenses please as supporting members. We have the authority to seek and have answers met. Best regards, Chad M Sent from my iPhone > On Jan 7, 2016, at 6:00 PM, Walter Benton via Histonet wrote: > > The website has some of the information you are speaking about, but I don't think tax statements are usually provided. They have the financials there for your review and I have included the link as well. > > http://www.nsh.org/sites/default/files/2014%20Annual%20Report%20Final.pdf > > > Walter Benton HT(ASCP)QIHC > Lab Operations Manager > Chesapeake Urology Associates > 806 Landmark Drive, Suite 127 > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > Chesapeakeurology.com > > Voted a Best Place to Work by > Baltimore and Modern Healthcare > Magazines. > ________________________________________ > From: WILLIAM DESALVO via Histonet > Sent: Thursday, January 7, 2016 4:31 PM > To: 'histonet at lists.utsouthwestern.edu' > Subject: [Histonet] NSH Financial and Tax Statements > > As a due paying member of NSH, I ask all members and anyone interested in Histotechnology request from NSH office to see the Financial and Tax Statements for 2013 / 2014. This is public information. We should all know where our dues money, money from educational activities and vendor money from the convention is being spent. > > Sent from my Windows Phone > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 8 Jan 2016 11:56:55 +0000 From: Steve McClain To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Histonet Digest, Vol 146, Issue 4 validation suggestions for short cycle processing with conventional equipment Message-ID: <5D4461F7-661B-4B33-9F3E-27D7679BF033 at mcclainlab.com> Content-Type: text/plain; charset="us-ascii" I posted our 7 year experience on this topic previously on histonet. Remember, this machine (K3000, VIP5) was not designed specifically for this purpose, but it can be made to work admirably. IOW Short processing on VIP is unreliable UNLESS you are willing to accept or control certain conditions, 1) only process well-fixed tissue 2) take formalin off this processor, making 14 changes beginning in 50%. If you want to keep station 1 formalin and standardize fixation with time X in formalin at temp Y, that may work. I prefer to change the tissues to 50% alcohol at the grossing bench so that all tissues are not only fixed, but rinsed in alcohol and started processing at the growing bench. This simplifies processing to dehydration, clearing and paraffin infiltration. Simple is reliable and short processing cycles on this machine leave little room for error. 2) Program heat to 50C in all alcohols and xylene. Mixing pressure and vacuum yes yes yes 4) Keep all solutions fresh, rotate after 200- 400 blocks. 5) Lay blocks out not more than 2 cassettes back to back, max 100 for VIP5. 4 hour is reliable for tissues up to 3mm thickness such as punch biopsy. 2 hour is reliable for up to 1.5 mm tissue thickness such as core biopsies and shave biopsies of skin. Method of processor validation for short cycle in machine previously validated in your lab. To validate create your evaluation form and find a set of cases with sufficient tissue small tissue to divide into 2 equal blocks. This can (and will) be done on clinical cases if you are careful. Print duplicate sets of cassettes. Mark one half of case 1 yellow and one half blue (tissues will be processed separately, 1 regular and one short cycle and then recombined into 1 final block at embedding). We held the short processed tissue cassettes in formalin and timed the short cycle to end when the regular program ends. Then the two blocks are recombined or embedded together so that direct comparisons/measures can be made on one H&E and one on your most common IHC for that tissue. If the H&E and IHC both work, chances are everything else will also. Modify your regular validation form or Create a form for scoring shrinkage, measured thickness and length, staining reactions, shrinkage, holes and flaws, have your path score. photo graph yellow and blue tissues under H&E and IHC. IF you do observe shrinkage or other undesirable artifact, be certain that your tissues are completely fixed at grossing and repeat. If they are comparable in the oculars and to the camera, then show your work and voila, there is your validation. Steve A. McClain, MD > ------------------------------ Message: 7 Date: Fri, 8 Jan 2016 13:26:53 +0000 From: "Sanders, Jeanine (CDC/OID/NCEZID)" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] lab humidifiers Message-ID: <3B2CD438E1628A41BD687E98B963B7813A5A9DF6 at EMBX-CLFT4.cdc.gov> Content-Type: text/plain; charset="us-ascii" Morning everyone! What is the standard in humidity levels that most histology labs strive for? And to achieve this goal, do any of you use a commercial-grade humidifier? Thanks for your help and have a nice weekend! Jeanine H. Sanders Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS-G32 Atlanta, GA 30329 ------------------------------ Message: 8 Date: Fri, 8 Jan 2016 13:49:18 +0000 From: Lester Raff MD To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Thanks for assistance Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B5F2347 at COLOEXCH01.uropartners.local> Content-Type: text/plain; charset="us-ascii" Thanks for spreading the word about block disposal. I have been inundated with requests, will have to sort through them all! For anyone interested Friday blog post http://www.chicagonow.com/downsize-maybe/2016/01/what-is-the-last-sound-you-will-hear/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ------------------------------ Message: 9 Date: Fri, 8 Jan 2016 14:42:33 +0000 (UTC) From: Histology Technician To: Histonet List , "histonet-request at lists.utsouthwestern.edu" Subject: [Histonet] Price gun Message-ID: <447350760.1678249.1452264153035.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 ? I was thinking of using a price gun to date my supplies when they come in, but I can't find one to buy.? Any ideas where to search for one?What do you guys use to date your supplies?? I've always used a pen to write the rcd date and my initials but thought a price gun would be quicker and neater :) Thanks! ------------------------------ Message: 10 Date: Fri, 8 Jan 2016 15:01:08 +0000 From: Michael Ann Jones To: "Sanders, Jeanine (CDC/OID/NCEZID)" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] lab humidifiers Message-ID: Content-Type: text/plain; charset="iso-8859-1" We are struggling with our humidity constantly. Colorado is dry in the winter. We use at least two humidifiers to try to keep humidity at minimum 30% due to equipment manufacturer?s specs. We have log sheets and thermometer/humidifier measurers to keep track of humidity. Standard for equipment seems to be 30 - 80% humidity per manufacturer?s specifications of processors, etc. Good luck Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com Providing collaborative diagnostic services, saving lives today and tomorrow. On 1/8/16, 6:26 AM, "Sanders, Jeanine (CDC/OID/NCEZID) via Histonet" wrote: >Morning everyone! > >What is the standard in humidity levels that most histology labs strive >for? And to achieve this goal, do any of you use a commercial-grade >humidifier? > >Thanks for your help and have a nice weekend! > > >Jeanine H. Sanders >Centers for Disease Control and Prevention >1600 Clifton Rd., NE MS-G32 >Atlanta, GA 30329 > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 8 Jan 2016 15:35:17 +0000 From: Edison Narvaez To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] HPV 16/18 background Message-ID: <10d75e4b1c4f450ca1a4686118969218 at RAMEXCHANGE.HPS.LOCAL> Content-Type: text/plain; charset="us-ascii" Hello, I'm running HPV probes from Enzo on my Leica instruments, and we are noticing background episomal pattern on 16/18 staining of cases that are strongly positive for HPV 6/11, but not on cases that are 6/11 or 16/18 negative. Is anybody experiencing this same issue and are there any suggestions. I'm using Enzo probes, Enzo Stringency wash, Enzo mouse-antibiotin, and a polymer detection kit. Thank you ________________________________ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ------------------------------ Message: 12 Date: Fri, 8 Jan 2016 10:31:34 -0500 From: "Pam Barker" To: "Histonet" Subject: [Histonet] Happy New Year! Here's to a Fantastic 2016! Message-ID: <00b601d14a29$a87eeca0$f97cc5e0$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters! Happy New Year!!!! Here's to a terrific 2016!!!!!!! How did you ring in the New Year? I hope you had fun ushering out 2015 and welcoming in 2016. I know I am looking forward to an exciting new year. I wanted to drop you this quick line to let you know that I have been chatting with clients and have some exciting new opportunities. Here is a list of my current open positions: Leadership: Histology Lab Manager - Modesto, CA Histology Supervisor (Dermpath) - Fayetteville, AR Senior Histotech - Norfolk, VA B.S. required Histotechnicians/Histotechnologists: Histology Tech - Norfolk, VA 15K Sign on Bonus Histology Tech - Modesto, CA Mohs Tech - Naples, FL HT/HTL - Milwaukee, WI Dermpath Histotech - Kansas City, KS Histotech - Louisville, KY (learn electron microscopy) Dermpath Histotech - Tyler, TX Histotech - San Diego, CA Tech Support - Los Angeles, CA All of these are full time positions and my clients offer excellent compensation, benefits and in most cases relocation assistance and or a sign on bonus. For more information please contact me by e-mail: relia1 at earthlink.net or toll free: 866-607-3542 or you can reach me on my cell/text: 407-353-5070. If none of these positions interest you and you are on the hunt for a new position NOW please drop me an email and let me know what you are looking for. My phone is ringing off the hook and your next opportunity could be just a phone call away. When I get the call I want to know to call YOU! If you are set in your current position Congratulations! and thank you for taking the time to read my email. I have new positions coming in all of the time and you never know when something might interest you or a friend or coworker. The best part is if you refer someone and I place them you will earn a referral fee! So please stay tuned for my next email! Happy New Year to You & Yours!! I wish you a year of love, laughter, good health & prosperity. Thanks-Pam Happy New Year! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 13 Date: Fri, 8 Jan 2016 15:32:04 +0000 From: "Sanders, Jeanine (CDC/OID/NCEZID)" To: Michael Ann Jones , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] lab humidifiers Message-ID: <3B2CD438E1628A41BD687E98B963B7813A5A9F3A at EMBX-CLFT4.cdc.gov> Content-Type: text/plain; charset="iso-8859-1" Thanks. We dog log the temp. I have had several service techs come in and comment on how much static we have. It has been worse than usual lately so am looking into options. Thanks much for your input! ________________________________________ From: Michael Ann Jones [mjones at metropath.com] Sent: Friday, January 08, 2016 10:01 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] lab humidifiers We are struggling with our humidity constantly. Colorado is dry in the winter. We use at least two humidifiers to try to keep humidity at minimum 30% due to equipment manufacturer?s specs. We have log sheets and thermometer/humidifier measurers to keep track of humidity. Standard for equipment seems to be 30 - 80% humidity per manufacturer?s specifications of processors, etc. Good luck Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com Providing collaborative diagnostic services, saving lives today and tomorrow. On 1/8/16, 6:26 AM, "Sanders, Jeanine (CDC/OID/NCEZID) via Histonet" wrote: >Morning everyone! > >What is the standard in humidity levels that most histology labs strive >for? And to achieve this goal, do any of you use a commercial-grade >humidifier? > >Thanks for your help and have a nice weekend! > > >Jeanine H. Sanders >Centers for Disease Control and Prevention >1600 Clifton Rd., NE MS-G32 >Atlanta, GA 30329 > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 146, Issue 6 **************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. From terry.dunn at roche.com Fri Jan 8 13:07:20 2016 From: terry.dunn at roche.com (Dunn, Terry) Date: Fri, 8 Jan 2016 14:07:20 -0500 Subject: [Histonet] Pricing Gun Message-ID: Staples and Office Depot carry them. They seem to run $100-200. Terry Dunn Sr. Manager, Workflow Consulting Roche Diagnostics Direct (520) 730-9596 terry.dunn at roche.com From tbraud at holyredeemer.com Fri Jan 8 14:25:09 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 8 Jan 2016 20:25:09 +0000 Subject: [Histonet] better than a price gun Message-ID: <48E053DDF6CE074DB6A7414BA05403F8056F06@HRHEX02-HOS.holyredeemer.local> UAL (United Ad Label) has a label guns, similar to the old paper labeler which puts wonderful labels, your choice of rec'vd date, date open, date exp...or any combination of the 3. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 9. Price gun (Histology Technician) Message: 9 Date: Fri, 8 Jan 2016 14:42:33 +0000 (UTC) ? I was thinking of using a price gun to date my supplies when they come in, but I can't find one to buy.? Any ideas where to search for one?What do you guys use to date your supplies?? I've always used a pen to write the rcd date and my initials but thought a price gun would be quicker and neater :) Thanks! ------------------------------ Message: 10 Date: Fri, 8 Jan 2016 15:01:08 +0000 From: Michael Ann Jones To: "Sanders, Jeanine (CDC/OID/NCEZID)" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] lab humidifiers Message-ID: Content-Type: text/plain; charset="iso-8859-1" We are struggling with our humidity constantly. Colorado is dry in the winter. We use at least two humidifiers to try to keep humidity at minimum 30% due to equipment manufacturer?s specs. We have log sheets and thermometer/humidifier measurers to keep track of humidity. Standard for equipment seems to be 30 - 80% humidity per manufacturer?s specifications of processors, etc. Good luck Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com Providing collaborative diagnostic services, saving lives today and tomorrow. On 1/8/16, 6:26 AM, "Sanders, Jeanine (CDC/OID/NCEZID) via Histonet" wrote: >Morning everyone! > >What is the standard in humidity levels that most histology labs strive >for? And to achieve this goal, do any of you use a commercial-grade >humidifier? > >Thanks for your help and have a nice weekend! > > >Jeanine H. Sanders >Centers for Disease Control and Prevention >1600 Clifton Rd., NE MS-G32 >Atlanta, GA 30329 > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 8 Jan 2016 15:35:17 +0000 From: Edison Narvaez To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] HPV 16/18 background Message-ID: <10d75e4b1c4f450ca1a4686118969218 at RAMEXCHANGE.HPS.LOCAL> Content-Type: text/plain; charset="us-ascii" Hello, I'm running HPV probes from Enzo on my Leica instruments, and we are noticing background episomal pattern on 16/18 staining of cases that are strongly positive for HPV 6/11, but not on cases that are 6/11 or 16/18 negative. Is anybody experiencing this same issue and are there any suggestions. I'm using Enzo probes, Enzo Stringency wash, Enzo mouse-antibiotin, and a polymer detection kit. Thank you ________________________________ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ------------------------------ Message: 12 Date: Fri, 8 Jan 2016 10:31:34 -0500 From: "Pam Barker" To: "Histonet" Subject: [Histonet] Happy New Year! Here's to a Fantastic 2016! Message-ID: <00b601d14a29$a87eeca0$f97cc5e0$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters! Happy New Year!!!! Here's to a terrific 2016!!!!!!! How did you ring in the New Year? I hope you had fun ushering out 2015 and welcoming in 2016. I know I am looking forward to an exciting new year. I wanted to drop you this quick line to let you know that I have been chatting with clients and have some exciting new opportunities. Here is a list of my current open positions: Leadership: Histology Lab Manager - Modesto, CA Histology Supervisor (Dermpath) - Fayetteville, AR Senior Histotech - Norfolk, VA B.S. required Histotechnicians/Histotechnologists: Histology Tech - Norfolk, VA 15K Sign on Bonus Histology Tech - Modesto, CA Mohs Tech - Naples, FL HT/HTL - Milwaukee, WI Dermpath Histotech - Kansas City, KS Histotech - Louisville, KY (learn electron microscopy) Dermpath Histotech - Tyler, TX Histotech - San Diego, CA Tech Support - Los Angeles, CA All of these are full time positions and my clients offer excellent compensation, benefits and in most cases relocation assistance and or a sign on bonus. For more information please contact me by e-mail: relia1 at earthlink.net or toll free: 866-607-3542 or you can reach me on my cell/text: 407-353-5070. If none of these positions interest you and you are on the hunt for a new position NOW please drop me an email and let me know what you are looking for. My phone is ringing off the hook and your next opportunity could be just a phone call away. When I get the call I want to know to call YOU! If you are set in your current position Congratulations! and thank you for taking the time to read my email. I have new positions coming in all of the time and you never know when something might interest you or a friend or coworker. The best part is if you refer someone and I place them you will earn a referral fee! So please stay tuned for my next email! Happy New Year to You & Yours!! I wish you a year of love, laughter, good health & prosperity. Thanks-Pam Happy New Year! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 13 Date: Fri, 8 Jan 2016 15:32:04 +0000 From: "Sanders, Jeanine (CDC/OID/NCEZID)" To: Michael Ann Jones , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] lab humidifiers Message-ID: <3B2CD438E1628A41BD687E98B963B7813A5A9F3A at EMBX-CLFT4.cdc.gov> Content-Type: text/plain; charset="iso-8859-1" Thanks. We dog log the temp. I have had several service techs come in and comment on how much static we have. It has been worse than usual lately so am looking into options. Thanks much for your input! ________________________________________ From: Michael Ann Jones [mjones at metropath.com] Sent: Friday, January 08, 2016 10:01 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] lab humidifiers We are struggling with our humidity constantly. Colorado is dry in the winter. We use at least two humidifiers to try to keep humidity at minimum 30% due to equipment manufacturer?s specs. We have log sheets and thermometer/humidifier measurers to keep track of humidity. Standard for equipment seems to be 30 - 80% humidity per manufacturer?s specifications of processors, etc. Good luck Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com Providing collaborative diagnostic services, saving lives today and tomorrow. On 1/8/16, 6:26 AM, "Sanders, Jeanine (CDC/OID/NCEZID) via Histonet" wrote: >Morning everyone! > >What is the standard in humidity levels that most histology labs strive >for? And to achieve this goal, do any of you use a commercial-grade >humidifier? > >Thanks for your help and have a nice weekend! > > >Jeanine H. Sanders >Centers for Disease Control and Prevention >1600 Clifton Rd., NE MS-G32 >Atlanta, GA 30329 > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 146, Issue 6 **************************************** From Steven.Swartwood at cshs.org Fri Jan 8 15:30:17 2016 From: Steven.Swartwood at cshs.org (Swartwood, Steven J) Date: Fri, 8 Jan 2016 21:30:17 +0000 Subject: [Histonet] Androgen receptor antibody raised against mouse Message-ID: <959202AC61AEF942968646EC66E2BE3EAD85C7FD@ESPWMSGMBX08.CSMC.EDU> Hello all, Does anyone have a recommendation for an anti-mouse Androgen Receptor antibody for Immunohistochemistry on formalin fixed paraffin embedded mouse tissues? We have some mouse prostate tumor models that we're working with. Again we are trying to stain for Androgen Receptor in a mouse, and not the human AR. I have reviewed a few papers and have some decent options, but I'd like to see if the histonet community has any expertise with this. Thank you for all your time and efforts as you always help me out, Steven Swartwood HT (ASCP) Cedars-Sinai Medical Center IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is strictly prohibited. Thank you for your cooperation. From tkngflght at yahoo.com Sat Jan 9 13:06:03 2016 From: tkngflght at yahoo.com (Cheryl) Date: Sat, 9 Jan 2016 11:06:03 -0800 Subject: [Histonet] received dates no longer required Message-ID: <03D9FBAE-53EB-4367-B5E5-6A5FD2107997@yahoo.com> Hi guys- I'm probably coming in a little late to this conversation. Received dates are no longer required by any of the governing bodies. Haven't been for a while. Opened dates aren't required either, as long as the reagent's performance isn't altered by the mechanical act of opening the container (this does apply to some clinical reagent's and almost no histology supplies when stored correctly) The only required date is expiry. If on the rare instance the manufacturer doesn't supply one, your institution would need to decide what works (1 year up to as many as 5 years) and include this statement in a policy. Then and only then would you need to mark the incoming supply with the received and internally determined expiration date. To complete the variables that may come up- we can no longer extend and expiration by freezing. This used to be a common practice for concentrated antibodies to extend expiration, but no more. Take a minute and look it up for CAP, JCACO and all the others-- it'll save you SO much time -- don't forget to update your policies so you're not breaking your own internal guidelines. The only reason I can think of to date everything is if folks don't reliably rotate stock-- and even if they are-- if your supplier isn't doing it well, your applied dates might not line up with first expired-- Thoughts? Cheryl From litepath2000 at yahoo.com Mon Jan 11 07:45:36 2016 From: litepath2000 at yahoo.com (NYSHisto) Date: Mon, 11 Jan 2016 13:45:36 +0000 (UTC) Subject: [Histonet] Obtaining Controls References: <906067665.3369750.1452519936353.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <906067665.3369750.1452519936353.JavaMail.yahoo@mail.yahoo.com> Does anyone know of any references or specific guidelines (CAP or CLIA) regarding the use and collection of control tissue from patient samples? TIA From carrie.schray at gmail.com Mon Jan 11 08:09:10 2016 From: carrie.schray at gmail.com (Carrie Schray) Date: Mon, 11 Jan 2016 09:09:10 -0500 Subject: [Histonet] Histology Position at Univ. of Michigan Message-ID: Applications being accepted for a full time research histotechnologist at the Univ. of Michigan in Ann Arbor. Please review posting #120103 at www.umjobs.org. From histo at pathlab.us Mon Jan 11 08:23:59 2016 From: histo at pathlab.us (Histology) Date: Mon, 11 Jan 2016 14:23:59 +0000 Subject: [Histonet] GI biopsies processing Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD822BBDA2@vdc01.domain.local> Hi All, Can anyone share their processing set up for GI biopsies? No matter what I do, our tissue seems dry. Any input would be great. Thanks in advance, Mehndi Helgren Dominion Pathology Labs. From j.rowaihi at alborglaboratories.com Mon Jan 11 08:51:01 2016 From: j.rowaihi at alborglaboratories.com (Jamal) Date: Mon, 11 Jan 2016 17:51:01 +0300 Subject: [Histonet] Diapath Histology Machines Message-ID: <006001d14c7f$7e41e3e0$7ac5aba0$@rowaihi@alborglaboratories.com> Hi Histonet Anyone have experience about the Diapath Histology Machines . Automatic Tissue Processor For Histology. Specimen., DONATELLO, open system 52PRO03 . Semi-automatic microtome Galileo SEMI - Diapath . CANOVA EMBEDDING MACHINE. MODULE (110-230V) 01pc. . CANOVA COLD PLATES MODULE (230 V) 01 pc. . Flotation bath DPH 35. Best Regards, Jamal Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories From LRaff at uropartners.com Mon Jan 11 08:51:08 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 11 Jan 2016 14:51:08 +0000 Subject: [Histonet] Antigen retrieval question. and a bit more Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B605EFA@COLOEXCH01.uropartners.local> Hello 'netters, hope you all had a good weekend. We are having some difficulties with the pressure cooker chambers we use for antigen retrieval. What different methodologies would be recommended? If we went to a different methodology for retrieval, would each antigen have to be revalidated? How many slides/runs would you recommend? Thanks. For any interested, new blog post http://www.chicagonow.com/downsize-maybe/2016/01/why-did-my-cat-dump-me/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From Royl1 at LabCorp.com Mon Jan 11 09:23:32 2016 From: Royl1 at LabCorp.com (Roy, Lisa) Date: Mon, 11 Jan 2016 15:23:32 +0000 Subject: [Histonet] Pancytokeratin (AE1/AE3) Protocol Message-ID: <2bcc8e7916194d3cb32fb55445c95f41@rtwems04.lca.labcorp.com> All of a sudden our AE1 is no longer good for the pathologists. Anyone willing to share their protocol for Ventana Benchmark XT or Ultra? I have "tweaked" this protocol so many times. It amazes me how often stains are great during optimization and validation, then months later, everyone hates it!!! UGH. Thanks Lisa Roy, HT(ASCP)cm -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. From Toni.McDaniel at ky.gov Mon Jan 11 10:02:06 2016 From: Toni.McDaniel at ky.gov (McDaniel, Toni (Justice)) Date: Mon, 11 Jan 2016 16:02:06 +0000 Subject: [Histonet] dry biopsie questions Message-ID: <5D09742D8BBF254588268A6C35B92CE2634C89BD@AGEXM01.eas.ds.ky.gov> Start with retrieval from biopsy what are they doing with the tissue immediately after removal from body Second, processing times are very important to. Our institution uses a short program with watching the time in formalin to be not less than 6 hours. (This is For tissue that is 3 mm or less aslo) Our program is only 2 hours total processing time. If you want the program email me at tlmcda02 at louisville.edu And I will email it directly to you \ From tejohnson at genoptix.com Mon Jan 11 10:32:36 2016 From: tejohnson at genoptix.com (Teri Johnson) Date: Mon, 11 Jan 2016 16:32:36 +0000 Subject: [Histonet] received dates no longer required Message-ID: <10d9fe4b41254f54800e3c1d5d34f065@PHUSCB-SP37MB03.genoptix.org> As of January 2016, the updated NY State Dept of Health standards still require received date, and date the material is placed in service or opened: Reagents Sustaining Standard of Practice 4 (REAG S4): Inventory Control There shall be an inventory control system for supplies. This system should include the recording of lot numbers and expiration dates of all relevant reagents, control materials, and calibrators; the date of receipt in the laboratory; the date of performance verification and the date the material is placed in service. All of these quality records shall be available for laboratory management and Department review. -AND- Reagents Sustaining Standard of Practice 5 (REAG S5): Labeling Reagents, solutions, culture media, control materials, calibration materials, and other supplies, as appropriate, must be labeled to indicate the following: a) identity; b) titer, strength or concentration as applicable; c) storage conditions; d) preparation date or date opened and the identity of the preparer; e) unopened and opened expiration date if pertinent to the performance of the reagent; and, f) other relevant information. So if you have NY certification, you will still need to do this practice. An inspector could argue that f) other relevant information could include received date. Best wishes, Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com Message: 1 Date: Sat, 9 Jan 2016 11:06:03 -0800 From: Cheryl To: histonet at lists.utsouthwestern.edu Subject: [Histonet] received dates no longer required Message-ID: <03D9FBAE-53EB-4367-B5E5-6A5FD2107997 at yahoo.com> Content-Type: text/plain;charset=us-ascii Hi guys- I'm probably coming in a little late to this conversation. Received dates are no longer required by any of the governing bodies. Haven't been for a while. Opened dates aren't required either, as long as the reagent's performance isn't altered by the mechanical act of opening the container (this does apply to some clinical reagent's and almost no histology supplies when stored correctly) The only required date is expiry. If on the rare instance the manufacturer doesn't supply one, your institution would need to decide what works (1 year up to as many as 5 years) and include this statement in a policy. Then and only then would you need to mark the incoming supply with the received and internally determined expiration date. To complete the variables that may come up- we can no longer extend and expiration by freezing. This used to be a common practice for concentrated antibodies to extend expiration, but no more. Take a minute and look it up for CAP, JCACO and all the others-- it'll save you SO much time -- don't forget to update your policies so you're not breaking your own internal guidelines. The only reason I can think of to date everything is if folks don't reliably rotate stock-- and even if they are-- if your supplier isn't doing it well, your applied dates might not line up with first expired-- Thoughts? Cheryl ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 146, Issue 8 **************************************** ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From Herrick.James at mayo.edu Mon Jan 11 11:39:51 2016 From: Herrick.James at mayo.edu (Herrick, James L. (Jim), MSA) Date: Mon, 11 Jan 2016 17:39:51 +0000 Subject: [Histonet] mouse femur decal and paraffin embedding protocol Message-ID: <7b6d90$274dcf@ironport10.mayo.edu> Dear Histonet Colleagues, I hope the holiday season has been good to all. We are interested in a routinely used protocol to decal, process and paraffin embed mouse femur and tibia. Some of our projects may require a gentle decal, whereas others may not be so delicate. Also, we do have access to a microwave for decalcifying, if recommended to be a better method. It would certainly save a lot of time. It would be greatly appreciated if anyone would be willing to share a protocol for post-harvest sample storage (i.e. PBS, 70% ETOH, saline, etc.), decalcification, processing and embedding. Thank you in advance for your help!! Jim Herrick, M.S.A. Supervisor - Sr. Res.Tech. Department of Orthopedics Biomaterials and Histomorphometry Core Lab Biomaterials and Tissue Engineering Lab Office Phone: (507) 538-4300 Lab Phone: (507) 255-5946 Pager: 127 (13239) Fax: (507) 266-9451 Email: herrick.james at mayo.edu ______________________ Mayo Clinic 200 First Street SW Rochester, MN 55905 www.mayoclinic.org From liz at premierlab.com Mon Jan 11 12:07:44 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Mon, 11 Jan 2016 11:07:44 -0700 Subject: [Histonet] mouse femur decal and paraffin embedding protocol In-Reply-To: <7b6d90$274dcf@ironport10.mayo.edu> References: <7b6d90$274dcf@ironport10.mayo.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE028529166B57@SBS2K8.premierlab.local> James We use 10% Formic Acid to decal mouse femur and tibia - The rear legs are removed at the hip with a pair of rongeurs the skin is removed to the ankle, we allow the knee joints to fix for 48 hours, they need to be in a normal degree of flexion, meaning you want them in a container that does not bend the knee. We trim off the muscle and place in decal for overnight - process the next day on an hour processing cycle. You don't need microwave for mouse knees they decal quickly. Most of the work we do is for cartilage degeneration, rheumatoid or osteoarthritis, if you are looking at bone marrow I would modify slightly. I can send a work instruction document that has more information in another e-mail if you are interested. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Herrick, James L. (Jim), MSA via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, January 11, 2016 10:40 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] mouse femur decal and paraffin embedding protocol Dear Histonet Colleagues, I hope the holiday season has been good to all. We are interested in a routinely used protocol to decal, process and paraffin embed mouse femur and tibia. Some of our projects may require a gentle decal, whereas others may not be so delicate. Also, we do have access to a microwave for decalcifying, if recommended to be a better method. It would certainly save a lot of time. It would be greatly appreciated if anyone would be willing to share a protocol for post-harvest sample storage (i.e. PBS, 70% ETOH, saline, etc.), decalcification, processing and embedding. Thank you in advance for your help!! Jim Herrick, M.S.A. Supervisor - Sr. Res.Tech. Department of Orthopedics Biomaterials and Histomorphometry Core Lab Biomaterials and Tissue Engineering Lab Office Phone: (507) 538-4300 Lab Phone: (507) 255-5946 Pager: 127 (13239) Fax: (507) 266-9451 Email: herrick.james at mayo.edu ______________________ Mayo Clinic 200 First Street SW Rochester, MN 55905 www.mayoclinic.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From srishan at mail.holyname.org Mon Jan 11 13:28:01 2016 From: srishan at mail.holyname.org (srishan at mail.holyname.org) Date: Mon, 11 Jan 2016 14:28:01 -0500 Subject: [Histonet] changing tissue processors Message-ID: Hi Histonetter, Does any one have guidelines, which indicates how often to change the tissue processors - Cassette Volume run, Make of processors etc Thank you Nirmala Srishan Histology Supervisor Holy Name Medical Center 718 Teaneck Road Teaneck NJ 07666 Lab: 201 833 3023 Office: 201 541 6328 Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From madeathridge at pastnashville.com Mon Jan 11 13:38:27 2016 From: madeathridge at pastnashville.com (Maryann Deathridge) Date: Mon, 11 Jan 2016 12:38:27 -0700 Subject: [Histonet] PR antibody Message-ID: <79fa4616cb5f40c3872a3e07aabcbe1f@pastnashville.com> Hello Histonetters- Does anyone use PR clone 1E2 for the ER/PR receptors? Name of a vendor? Thanks in advance. madeathridge at pastnashville.com From Richard.Cartun at hhchealth.org Mon Jan 11 13:39:28 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 11 Jan 2016 19:39:28 +0000 Subject: [Histonet] IgG4 Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B097D1D@HHCEXCHMB03.hhcsystem.org> I am curious to hear which clone people are using to detect IgG4-positive plasma cells in FFPE tissue. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From CObregon at mhs.net Mon Jan 11 13:42:47 2016 From: CObregon at mhs.net (Obregon, Cecilia) Date: Mon, 11 Jan 2016 19:42:47 +0000 Subject: [Histonet] IgG4 In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E6B097D1D@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E6B097D1D@HHCEXCHMB03.hhcsystem.org> Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F6696947B@MHSEXMB03.mhs.net> We are using the MRQ-44 from Cell Marque. ________________________________________ From: Cartun, Richard via Histonet [histonet at lists.utsouthwestern.edu] Sent: Monday, January 11, 2016 2:39 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IgG4 I am curious to hear which clone people are using to detect IgG4-positive plasma cells in FFPE tissue. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. From mjones at metropath.com Mon Jan 11 14:20:51 2016 From: mjones at metropath.com (Michael Ann Jones) Date: Mon, 11 Jan 2016 20:20:51 +0000 Subject: [Histonet] changing tissue processors In-Reply-To: References: Message-ID: Nirmala, We use the number of blocks to guide on how often to change the processors. We experimented with how many blocks we could get away with and then backed the number up by 50 blocks to be sure. VIP5/6 for us is currently 700 blocks. Beyond that, tissues are not thoroughly processed. We used to do the change weekly, but we felt this was more accurate, wouldn?t waste reagents but ensure processed specimens. Good luck Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com Providing collaborative diagnostic services, saving lives today and tomorrow. On 1/11/16, 12:28 PM, "Nirmala Srishan via Histonet" wrote: >Hi Histonetter, > > >Does any one have guidelines, which indicates how often to change the >tissue processors - Cassette Volume run, Make of processors etc > >Thank you > > >Nirmala Srishan >Histology Supervisor >Holy Name Medical Center >718 Teaneck Road >Teaneck NJ 07666 >Lab: 201 833 3023 >Office: 201 541 6328 > > > > > > > >Holy Name Medical Center is ranked among the top hospitals in the nation >for patient care, clinical performance and workplace excellence. >Click here to learn more. > >**** Warning: The information contained in this message is privileged and >CONFIDENTIAL and is intended only for the use of the addressee above. If >you are not the intended recipient, you are hereby notified that any >disclosure, copying, distribution, or taking of any action in reliance on >the content of this message is strictly prohibited. If you have received >this communication in error, please notify the sender by replying to this >message, and then delete it from your system. > > > > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac at outlook.com Mon Jan 11 14:45:21 2016 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Mon, 11 Jan 2016 13:45:21 -0700 Subject: [Histonet] changing tissue processors Message-ID: I would suggest by cassette numbers. It will take some data collection, but once you settle on the number of cassettes, then you will have the process in control. The actual number will depend on the tissue types placed in the cassettes and the amount of blood, lipids and other elements extracted. Start with a minimum of 600 cassettes and see see what additional cassettes can be processed without losing quality. Sent from my Windows Phone ________________________________ From: Nirmala Srishan via Histonet Sent: ?1/?11/?2016 12:33 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] changing tissue processors Hi Histonetter, Does any one have guidelines, which indicates how often to change the tissue processors - Cassette Volume run, Make of processors etc Thank you Nirmala Srishan Histology Supervisor Holy Name Medical Center 718 Teaneck Road Teaneck NJ 07666 Lab: 201 833 3023 Office: 201 541 6328 Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght at yahoo.com Mon Jan 11 21:31:30 2016 From: tkngflght at yahoo.com (Cheryl) Date: Mon, 11 Jan 2016 19:31:30 -0800 Subject: [Histonet] Received date. Message-ID: <085D3164-28E2-4302-9643-6C83F77223D2@yahoo.com> Hi Terri - That list is specific to New York State, no other state is so restrictive, and it doesn't cite 'received date' as a requirement. If you've got all that other stuff-- which is generally included on reagent manufacture labels with minor exception-- I can't think of what value a received date would add. I'd not go to all that effort without a clarification from the state. Most inspection agencies aren't trying to create work for no gain. The takeaway? With all the licensure restrictions, state-specific regulations and remarkable amounts of snow- y'all have it ROUGH! Cheryl Please excuse typos-sent from a phone. From criley at dpspa.com Tue Jan 12 07:28:54 2016 From: criley at dpspa.com (Charles Riley) Date: Tue, 12 Jan 2016 08:28:54 -0500 Subject: [Histonet] Disposal of blocks and slides Message-ID: Hello all, What are the guidelines for disposal of blocks and slides? This was never discussed in my program and I am now in charge of the department. No one who currently works here has been through the process. Any help will greatly be appreciated. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE From NMargaryan at luriechildrens.org Tue Jan 12 11:08:37 2016 From: NMargaryan at luriechildrens.org (Margaryan, Naira) Date: Tue, 12 Jan 2016 17:08:37 +0000 Subject: [Histonet] rat vomeronasal organ (VNO) fix, decal, proc. and paraffin embedding protocol Message-ID: <34B2EDA118548A4EB35D6E650345BA645E7EE8A6@SV-EX08.childrensmemorial.org> Dear Histonet Colleagues, I hope you had very nice and warm holiday season . We are interested in a routinely used protocol to decal (if needed), process and paraffin embed the rat vomeronasal organ (VNO). It would be greatly appreciated if anyone would be willing to share a protocol for post-harvest sample storage (i.e. PBS, 70% ETOH, saline, etc.), fixation, decalcification, processing before embedding. Thanks in advance for your help!! Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) From bertjekb at gmail.com Tue Jan 12 11:58:38 2016 From: bertjekb at gmail.com (B kB) Date: Tue, 12 Jan 2016 18:58:38 +0100 Subject: [Histonet] Disposal of blocks and slides In-Reply-To: References: Message-ID: Hello, in the Netherlands (Europe), the Dutch Society of Pathologists, has made a document called "code for good use of body materials". This advice is established after an juridical consultation in connection with the excisting legal provisions. The standard for now (since 2011) is: blocks 115 years frozen material 30 years slides (glass or digital) 30 years reports 30 years Bert klein Brink Head of Department Histopathology Gelre Hospital Apeldoorn (Netherlands) Op 12 jan. 2016 14:34 schreef "Charles Riley via Histonet" < histonet at lists.utsouthwestern.edu>: > Hello all, > > What are the guidelines for disposal of blocks and slides? This was never > discussed in my program and I am now in charge of the department. No one > who currently works here has been through the process. Any help will > greatly be appreciated. > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > > Doctors Pathology Services, Dover DE > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ahughes8 at ITS.JNJ.com Tue Jan 12 12:15:38 2016 From: ahughes8 at ITS.JNJ.com (Hughes, Anna [JRDUS]) Date: Tue, 12 Jan 2016 18:15:38 +0000 Subject: [Histonet] anti p53 Message-ID: <1062C7BE2E82104EBB0231BDCBE87E2F09CDE20A@ITSUSRAGMDGE05.jnj.com> Hi Histo Peeps! Anyone have a recommendation for an anti human p53 antibody? Thanks in advance for your help! Anna Anna Hughes ahughes8 at its.jnj.com ************************************************** Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Johnson & Johnson can arrange for proper delivery, and then please delete the message from your inbox. Thank you. From wbenton at cua.md Tue Jan 12 12:34:52 2016 From: wbenton at cua.md (Walter Benton) Date: Tue, 12 Jan 2016 18:34:52 +0000 Subject: [Histonet] Disposal of blocks and slides In-Reply-To: References: Message-ID: <3f62164a78904c8bb752d3e1554b340c@MAIL01.GCU-MD.local> Charles, Not sure if you are CAP accredited or will be, but this is from our checklist for the last inspection period. ANP.12500 Record Retention Phase II Surgical pathology records and materials are retained for an appropriate period. NOTE 1: Minimum requirements for surgical pathology, providing these are not less stringent than local, state and national regulations, are: 1. Accession log records - 2 years 2. Wet tissue (stock bottle) - 2 weeks after final report 3. Paraffin blocks - 10 years (subject to Notes 2 and 3, below) 4. Glass slides (including control slides) and reports - 10 years (slides must remain readable for this period) 5. Surgical pathology reports - 10 years (see Notes 4 and 5, below) 6. Fluorochrome-stained slides - at the discretion of the laboratory director 7. Fine needle aspiration slides - 10 years 8. Images of FISH studies - 10 years (see Note 6, below) There must be a documented policy for protecting and preserving the integrity and retrieval of surgical pathology materials and records. The retention period should be extended, when appropriate, to provide documentation for adequate quality control and medical care. NOTE 2: Regarding extra-institutional release of blocks for research purposes: Federal regulations require that a laboratory retain paraffin blocks for two years unless the tissue is blocked specifically for research and not used for patient diagnostic purposes.* The CAP Commission on Laboratory Accreditation (CLA) requires, however, that paraffin blocks used for patient diagnostic purposes must be kept for at least 10 years. Nevertheless, such blocks may be released for research purposes after the two-year regulatory requirement if all of the following criteria are met: 1. For laboratories subject to US regulations, formal written authorization is obtained in accordance with the requirements of HIPAA if identifiable patient information is released. 2. The laboratory retains sufficient blocks to support the diagnosis for the full 10-year period. 3. Provision is made for retrieval by the laboratory of any blocks or material that remain after use in research, if the blocks or material are needed for diagnostic, legal, or other legitimate purposes. 4. The laboratory meets other relevant requirements including but not limited to the requirements of the institution, the directives of any applicable institutional review board (IRB) or similar entity; and state and local laws and regulations. NOTE 3: Given that patient survival rates are increasing and the continued emergence of treatment based on biomarker testing, which at times may be required on the original tissue, it is recommended that, whenever feasible, tissue block retention from patients with diagnosed malignancies be retained beyond the 10 year requirement. NOTE 4: Pathology reports may be retained in either paper or electronic format. If retained in electronic format alone, however, the electronic reports must include a secure pathologist electronic signature. Images of paper reports--such as microfiche or PDF files--are acceptable. NOTE 5: Reports of outside consultations performed on cases from the laboratory (whether or not such consultation was requested by the laboratory) must be retained for 10 years after the date on which the original report was issued. NOTE 6: There is no retention requirement for images when the source slides remain readable for the required 10-year retention period. The 10-year retention requirement applies to images of slide preparations that are not readable for the 10-year period (e.g. FISH studies). *The restriction on release of blocks does not prohibit release of blocks for purposes of treatment, diagnosis, prognosis, etc., for patients on research protocols as long as release is consistent with patient privacy regulations (e.g. HIPAA) and applicable state and local Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, January 12, 2016 8:29 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Disposal of blocks and slides Hello all, What are the guidelines for disposal of blocks and slides? This was never discussed in my program and I am now in charge of the department. No one who currently works here has been through the process. Any help will greatly be appreciated. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From agleiberman at buffalobiolabs.com Tue Jan 12 12:40:39 2016 From: agleiberman at buffalobiolabs.com (Anatoli Gleiberman) Date: Tue, 12 Jan 2016 18:40:39 +0000 Subject: [Histonet] anti p53 In-Reply-To: <1062C7BE2E82104EBB0231BDCBE87E2F09CDE20A@ITSUSRAGMDGE05.jnj.com> References: <1062C7BE2E82104EBB0231BDCBE87E2F09CDE20A@ITSUSRAGMDGE05.jnj.com> Message-ID: DO-1 mouse monoclonal anti-human p53 either from Santa Cruz or from Abcam are the best. Anatoli Gleiberman, PhD Director of Histopathology Buffalo Biolabs LLC, 73 High Street Buffalo, NY, 14203 Phone:716-849-6810x354 e-mail:agleiberman at buffalobiolab.com -----Original Message----- From: Hughes, Anna [JRDUS] via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, January 12, 2016 1:16 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] anti p53 Hi Histo Peeps! Anyone have a recommendation for an anti human p53 antibody? Thanks in advance for your help! Anna Anna Hughes ahughes8 at its.jnj.com ************************************************** Confidentiality Notice: This e-mail transmission may contain confidential or legally privileged information that is intended only for the individual or entity named in the e-mail address. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or reliance upon the contents of this e-mail is strictly prohibited. If you have received this e-mail transmission in error, please reply to the sender, so that Johnson & Johnson can arrange for proper delivery, and then please delete the message from your inbox. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hist10 at aol.com Tue Jan 12 12:57:42 2016 From: hist10 at aol.com (Hist10) Date: Tue, 12 Jan 2016 13:57:42 -0500 Subject: [Histonet] Disposal of blocks and slides In-Reply-To: References: Message-ID: <15237336992-3daf-4863@webprd-m31.mail.aol.com> The New York state Law it is 20 for blocks and 20 year for slide also includes frozen sections slide Sent from AOL Mobile Mail Get the new AOL app: mail.mobile.aol.com On Tuesday, January 12, 2016, B kB via Histonet wrote: Hello, in the Netherlands (Europe), the Dutch Society of Pathologists, has made a document called "code for good use of body materials". This advice is established after an juridical consultation in connection with the excisting legal provisions. The standard for now (since 2011) is: blocks 115 years frozen material 30 years slides (glass or digital) 30 years reports 30 years Bert klein Brink Head of Department Histopathology Gelre Hospital Apeldoorn (Netherlands) Op 12 jan. 2016 14:34 schreef "Charles Riley via Histonet" < histonet at lists.utsouthwestern.edu>: > Hello all, > > What are the guidelines for disposal of blocks and slides? This was never > discussed in my program and I am now in charge of the department. No one > who currently works here has been through the process. Any help will > greatly be appreciated. > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > > Doctors Pathology Services, Dover DE > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dfayad at bidmc.harvard.edu Tue Jan 12 13:19:42 2016 From: dfayad at bidmc.harvard.edu (Fayad,Donna A. (BIDMC - Pathology)) Date: Tue, 12 Jan 2016 19:19:42 +0000 Subject: [Histonet] Radiation Message-ID: <46b35e8f8e5a4d0b92967f7d4563a83d@SR81BREEDS.its.caregroup.org> Does anyone have a policy for a histotech who handles blocks of patients exposed to radiation prior to surgery? Donna Fayad Anatomical Pathology Manager Beth Israel Deaconess Medical Center 330 Brookline Avenue Boston, Massachusetts 02215 P 617-667-7831 F 617-17-975-5620 PAGER 93341 ________________________________ This message is intended for the use of the person(s) to whom it may be addressed. It may contain information that is privileged, confidential, or otherwise protected from disclosure under applicable law. If you are not the intended recipient, any dissemination, distribution, copying, or use of this information is prohibited. If you have received this message in error, please permanently delete it and immediately notify the sender. Thank you. From brett_connolly at merck.com Tue Jan 12 14:57:40 2016 From: brett_connolly at merck.com (Connolly, Brett M) Date: Tue, 12 Jan 2016 15:57:40 -0500 Subject: [Histonet] PDGFR beta antibody for IHC Message-ID: Anyone have a favorite for rodent FFPE tissue? Thanks, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From victor_tobias at comcast.net Tue Jan 12 16:56:50 2016 From: victor_tobias at comcast.net (victor_tobias at comcast.net) Date: Tue, 12 Jan 2016 22:56:50 +0000 (UTC) Subject: [Histonet] Path Report Signout Location In-Reply-To: <2010785864.446348.1452639280735.JavaMail.zimbra@comcast.net> Message-ID: <246232883.447125.1452639410110.JavaMail.zimbra@comcast.net> HELP!! ? Looking for the CLIA or CAP wording on what is needed on the Path report. We are in the process of redesigning our reports, but I haven't been able to find the specifics? ? Victor From SteveM at mcclainlab.com Tue Jan 12 18:06:18 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Wed, 13 Jan 2016 00:06:18 +0000 Subject: [Histonet] Histonet Digest, Vol 146, Issue 10 tissue processors In-Reply-To: References: Message-ID: Sakura VIP you can run for decades if maintained. Extremely reliable. The parts are available. I have 3 K series and a VIP5. The newest is 17 years old, the oldest maybe 35 years. All acquired used and refurbished. Our newest processor, 7 years, a demo acquired almost new, failed miserably twice this year after 2000 processing runs. Began leaking from multiple sources and reagents, spent hours. Troubleshooting, 2 service calls, etc. I won't name the brand, but every single reagent bottle failed. New isn't always more reliable than old VIP. Steve A. McClain, MD PS extremely From carl.hobbs at kcl.ac.uk Wed Jan 13 04:09:28 2016 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Wed, 13 Jan 2016 10:09:28 +0000 Subject: [Histonet] IgG4 Message-ID: Images of IgG4-positive cells in tonsil FFPWS on Histonet Images website Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From mpence at grhs.net Wed Jan 13 09:41:27 2016 From: mpence at grhs.net (Mike Pence) Date: Wed, 13 Jan 2016 15:41:27 +0000 Subject: [Histonet] IQCP Message-ID: Okay here is my question... I know that AP at this time does not have to do IQCP, but they say that they are going to look at AP down the road. Has anyone started doing any work on this yet? or maybe you already have an IQCP for AP you could share. Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 mpence at grhs.net | www.greatrivermedical.org. www.Facebook.com/GreatRiverHealthSystems | www.Twitter/GreatRiverMed Information in this communication, including attachments, is confidential and intended only for the addressee(s). This communication may contain privileged, confidential, proprietary or trade secret information entitled to protection or exemption from disclosure under law. If you are not an intended recipient, please know that any use, distribution or copying of this communication, or any action taken based on the information in this communication, is unauthorized and may be unlawful. If you received this communication in error, please notify the sender and delete this communication from your device. From Pat.Patterson at propath.com Wed Jan 13 10:19:13 2016 From: Pat.Patterson at propath.com (Pat Patterson) Date: Wed, 13 Jan 2016 16:19:13 +0000 Subject: [Histonet] IHC Tech - Dallas Texas Message-ID: <6DCB8B92D0138244B56CE8EACE0D458D3D47F315@Mail.propathlab.com> HISTOTECHNICIAN IMMUNOHISTOCHEMISTRY DEPARTMENT Join our IHC Lab Team and create quality Immunohistochemistry slides for the diagnosis and treatment of disease. ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas with IHC specimens coming from around the country. We're looking for an experienced Histology Technician or Technologist for our evening shift 4:00pm to 12:30am Monday through Friday The ideal candidate will have at least 4 years of Histology experience with paraffin microtomy of a variety of different tissue types. Hands on IHC experience is strongly desired, but if you are a great Microtomist with only a little IHC experience or knowledge - give us a call - we can train you! HT (ASCP) strongly desired. Responsibilities include slide preparation (paraffin and frozen sections), IHC and In Situ hybridization staining, antibody titer preparation, pre-analytic paperwork and computer entry, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. The hours for the position are 4:00 p.m. to 12:30 a.m. Monday through Friday. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EEO/AA-M/F/disability/protected veteran status Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to accessibility at propath.com. Feel free to contact me directly I if you have any questions. Pat Patterson, HTL(ASCP) Manager, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From LRaff at uropartners.com Wed Jan 13 13:00:53 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 13 Jan 2016 19:00:53 +0000 Subject: [Histonet] Antigen retrieval Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B60CC77@COLOEXCH01.uropartners.local> Thanks for the input on ouor antigen retrieval problem. Biocare did a little troubleshooting for us, and things look great, at least for today. Is your lab playing the lottery? Our is: http://www.chicagonow.com/downsize-maybe/2016/01/sorry-gang-we-might-not-win-the-lottery-tonight/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From Fawn.Bomar at HalifaxRegional.com Thu Jan 14 08:48:11 2016 From: Fawn.Bomar at HalifaxRegional.com (Fawn Bomar) Date: Thu, 14 Jan 2016 14:48:11 +0000 Subject: [Histonet] Help on Competency Assessments Message-ID: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA19FF62@EXCH-2K10.hrhs.com> Hey everyone, I know that this question has been asked before and I have tried to find out information through the internet, but I am having a difficult time trying to find any type of sample forms. Would anyone be willing to share their competency assessment forms/checklists? I am trying to make one up for our lab and different areas and need some guidance to ensure that I cover everything that needs to be assessed. I know that I need to have the collection and transporting of specimens from the OR/Endo areas, receiving/accessioning and labeling specimens/blocks/slides. Preparing/sectioning/staining Frozens. Embedding/sectioning/staining routine stains/special stains/IHC stains. Documenting/performing of equipment maintenance/function checks/decons/temperature checks. Preparing and labeling cytology specimens received in lab/ prepared FNA slides/specimens from FNA's/ Prepared slides/specimens from CT guided procedures. I am having a hard time trying to figure out exactly how to document and which of the six (if not all) competency elements are needed to be checked for each of the areas. If anyone is willing to share their templates (and how they actually document, i.e., initial, checkmark) or perhaps point me to a website, book, or person to reference, I would be very grateful!!! Thank you, Fawn Bomar ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From gu.lang at gmx.at Thu Jan 14 09:02:55 2016 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 14 Jan 2016 16:02:55 +0100 Subject: [Histonet] additional barcode on ventana-labels Message-ID: <000001d14edc$a7617a10$f6246e30$@gmx.at> Hi! Did anyone manage to bring Ventana to open their label-design? We need an additional 2D-Barcode with the slide-data on the label. Regards Gudrun Lang From nlinke at sbch.org Thu Jan 14 11:59:28 2016 From: nlinke at sbch.org (Noelle Linke) Date: Thu, 14 Jan 2016 17:59:28 +0000 Subject: [Histonet] consult requests Message-ID: Hi all, Question for anyone who may handle admin staff: If a patient or an outside hospital/doctor requests a consult and you need to fedex slides/reports to another institution do you charge the patient to send these items or does your facility eat those charges? Thank you, No?lle No?lle Linke, MS, HTL(ASCP) QIHC Manager, Anatomic Pathology Pacific Diagnostic Laboratories Cottage Health System nlinke at sbch.org Phone: (805) 324-9814 Fax: (805) 696-6433 ________________________________ CH Disclaimer: This electronic mail message is intended exclusively for the individual or entity to which it is addressed. This message, together with any attachment, may contain confidential and privileged information. Any views, opinions or conclusions expressed in this message are those of the individual sender and do not necessarily reflect the views of Cottage Health, its subsidiaries or affiliates. This document may also contain information covered under the Health Insurance Portability and Accountability Act (HIPAA, PL 104-191) and implementing regulations and must be protected in accordance with those provisions. Re-disclosure without patient consent or as otherwise permitted by law is prohibited. Any unauthorized review, retransmission, use, printing, copying, retention, disclosure, distribution or the taking of any action in reliance upon this information by persons or entities other than the intended recipient is strictly prohibited and may be unlawful. If you have received this message in error, please immediately advise the sender by reply email message to the sender and delete all copies of this message from your system without copying. ________________________________ From rjbuesa at yahoo.com Thu Jan 14 12:20:17 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 14 Jan 2016 18:20:17 +0000 (UTC) Subject: [Histonet] consult requests In-Reply-To: References: Message-ID: <1218771994.4205925.1452795617091.JavaMail.yahoo@mail.yahoo.com> We used to charge the person (patient, hospital or pathologist) who requested the consult.Ren? On Thursday, January 14, 2016 1:01 PM, Noelle Linke via Histonet wrote: Hi all, Question for anyone who may handle admin staff:? If a patient or an outside hospital/doctor requests a consult and you need to fedex slides/reports to another institution do you charge the patient to send these items or does your facility eat those charges? Thank you, No?lle No?lle Linke, MS, HTL(ASCP) QIHC Manager, Anatomic Pathology Pacific Diagnostic Laboratories Cottage Health System nlinke at sbch.org Phone: (805) 324-9814 Fax: (805) 696-6433 ________________________________ CH Disclaimer: This electronic mail message is intended exclusively for the individual or entity to which it is addressed. This message, together with any attachment, may contain confidential and privileged information. Any views, opinions or conclusions expressed in this message are those of the individual sender and do not necessarily reflect the views of Cottage Health, its subsidiaries or affiliates. This document may also contain information covered under the Health Insurance Portability and Accountability Act (HIPAA, PL 104-191) and implementing regulations and must be protected in accordance with those provisions. Re-disclosure without patient consent or as otherwise permitted by law is prohibited. Any unauthorized review, retransmission, use, printing, copying, retention, disclosure, distribution or the taking of any action in reliance upon this information by persons or entities other than the intended recipient is strictly prohibited and may be unlawful. If you have received this message in error, please immediately advise the sender by reply email message to the sender and delete all copies of this message from your system without copying. ________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Thu Jan 14 13:11:08 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 14 Jan 2016 19:11:08 +0000 Subject: [Histonet] Consult Requests Message-ID: <48E053DDF6CE074DB6A7414BA05403F8057F7A@HRHEX02-HOS.holyredeemer.local> If a patient or an outside hospital/doctor requests a consult, we encourage patients to pick up their slides at the same time as they pick up radiology discs, medical records, etc. If we need to fedex slides/reports to another institution we ask the receiving facility for their Fed-Ex number. If they don't have one, then we eat the charge in favor of the patient getting the care they need. However, if we receive a request for a research project, or for duplication for legal reasons, then we charge a duplication fee for the cost of materials. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 4. consult requests (Noelle Linke) ------------------------------ Message: 4 Date: Thu, 14 Jan 2016 17:59:28 +0000 From: Noelle Linke Hi all, Question for anyone who may handle admin staff: If a patient or an outside hospital/doctor requests a consult and you need to fedex slides/reports to another institution do you charge the patient to send these items or does your facility eat those charges? Thank you, No?lle No?lle Linke, MS, HTL(ASCP) QIHC Manager, Anatomic Pathology Pacific Diagnostic Laboratories Cottage Health System nlinke at sbch.org Phone: (805) 324-9814 Fax: (805) 696-6433 From mills at 3scan.com Thu Jan 14 16:59:53 2016 From: mills at 3scan.com (Caroline Miller) Date: Thu, 14 Jan 2016 14:59:53 -0800 Subject: [Histonet] Paper request Message-ID: Hi All, Does anyone have access to this paper: Toluidine Blue-Alizarin Red S Staining of Cartilage and Bone in Whole-Mount Skeletons in Vitro Stain Technology Volume 40 , Issue 2 , 1965 Thanks in advance, I hope you are all doing well! yours, mills -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From mills at 3scan.com Thu Jan 14 18:35:19 2016 From: mills at 3scan.com (Caroline Miller) Date: Thu, 14 Jan 2016 16:35:19 -0800 Subject: [Histonet] Paper request In-Reply-To: References: Message-ID: Got it! Thanks Histonet!! :) mills On Thu, Jan 14, 2016 at 2:59 PM, Caroline Miller wrote: > Hi All, Does anyone have access to this paper: > Toluidine Blue-Alizarin Red S Staining of Cartilage and Bone in > Whole-Mount Skeletons in Vitro > > > Stain Technology Volume 40 > , Issue 2 > , 1965 > > > Thanks in advance, I hope you are all doing well! > > > yours, > > mills > > -- > Caroline Miller (mills) > Director of Histology > 3Scan.com > 415 2187297 > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From mcginnessjamie at yahoo.com Thu Jan 14 21:59:13 2016 From: mcginnessjamie at yahoo.com (Jamie McGinness) Date: Thu, 14 Jan 2016 21:59:13 -0600 Subject: [Histonet] Cryojane troubleshooting Message-ID: <810FF45C-1F2C-4E27-B7BA-FA2984D6F4E7@yahoo.com> I am using the cryojane and some of my tissue stays on the adhesive window when peeling it off the slide. I have tried 4x slides and activating the UV light longer. Wondering if I am removing the tape window the wrong way or does the cryostat temp need to be in a certain range for this to work. Any other thoughts appreciated. Sent from my iPhone From ENarvaez at Pathlinelabs.com Fri Jan 15 10:09:30 2016 From: ENarvaez at Pathlinelabs.com (Edison Narvaez) Date: Fri, 15 Jan 2016 16:09:30 +0000 Subject: [Histonet] PD-L1 protocol Message-ID: <85411025454247288d28b987ba8ceb8f@RAMEXCHANGE.HPS.LOCAL> Good Friday to all, I was wondering if anybody is running PD-L1/CD274 (SP142) from Spring Bioscience on Leica's Bond III. Any suggestions as per control tissue and protocol would be great. Thank you ________________________________ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. From dr at personifysearch.com Fri Jan 15 12:55:45 2016 From: dr at personifysearch.com (Danielle Robinson) Date: Fri, 15 Jan 2016 13:55:45 -0500 Subject: [Histonet] R&D Histotech Opportunity - Northern Chicago Suburbs Message-ID: Hello Histonet, Our exclusively retained client has opened a new histology opportunity and I wanted to reach out regarding a few specifics and see if anyone would be interested in learning more. Our client is a global leader in histology and IHC and they are looking for an R&D Histotech to work out of their facility North of Chicago. The R&D Histotech will be responsible for assisting in leading scientific research, development, and support for nominated products. The ideal candidate would have a strong background in embedding, processing, sectioning, and staining of tissue. This position is a full-time, direct-hire role with competitive salary and full benefits. If you or anyone you know may be interested in learning more about this position, please contact me directly at dr at personifysearch.com Thank you! Danielle Robinson Team Lead - Talent Acquisition 401 Harrison Oaks Boulevard, Suite 350 Cary, NC 27513 US Toll Free: +1 (800) 875-6188 ext. 140 International Access Phone: +1.919.473.3762 http:/ /w w w.personifysearch.com/ From mcginnessjamie at yahoo.com Fri Jan 15 13:44:03 2016 From: mcginnessjamie at yahoo.com (Jamie McGinness) Date: Fri, 15 Jan 2016 13:44:03 -0600 Subject: [Histonet] Cryojane and H&E staining Message-ID: <0A73C030-CDDF-4185-9131-720CE100204D@yahoo.com> I'm having issues with H&E staining from frozen fresh identical tissue cut using standard cryostat brush technique and cryojane technique. I have noticed the following. I do snap freeze tissue at -80 1. My slides from cryojane prepared tissue appear lighter than the same tissue cut with standard brush method in the cryostat when stained with H&E. 2. Tissue with cryojane method appears dehydrated (vacuoles, collagen splaying, etc) compared to the standard brush technique. Again all stained with H&E and side by side comparison of same tissue. Thanks for any thoughts or ideas. Sent from my iPhone From BZIMMERM at gru.edu Fri Jan 15 13:47:25 2016 From: BZIMMERM at gru.edu (Zimmerman, Billie) Date: Fri, 15 Jan 2016 19:47:25 +0000 Subject: [Histonet] GEORGIA SOCIETY FOR HISTOTECHNOLOGY HISTOPALOOZA III SAVE THE DATE!! Message-ID: This is a call for abstracts for our annual meeting being held at Lake Lanier Legacy Lodge, April 22-24, 2016. Also, please renew your membership. It's offered at the very lost cost of $0.00 It will be a great place to get CEUs and enjoy a nice peaceful weekend in a beautiful location. Billie Zimmerman GSH Secretary From naje1972 at yahoo.com Sat Jan 16 00:48:52 2016 From: naje1972 at yahoo.com (naje1972) Date: Sat, 16 Jan 2016 00:48:52 -0600 Subject: [Histonet] I would like get a processing schedule for GI's Message-ID: Sent from my Verizon Wireless 4G LTE Tablet From mcginnessjamie at yahoo.com Sat Jan 16 20:25:27 2016 From: mcginnessjamie at yahoo.com (Jamie McGinness) Date: Sat, 16 Jan 2016 20:25:27 -0600 Subject: [Histonet] Cryojane aqueous buffer salt mix Message-ID: <7E81CD23-241F-4984-A147-17A6E3F72613@yahoo.com> I'm having a lot of artifacts when using the cryojane system (collagen splaying, vacuoles, etc). I do not see this if I do standard brush technique on this frozen tissue. I am not using the Cryojane aqueous buffer salt mix. Is this something that helps with this. If not what is this Cryojane aqueous buffer salt mix for? Sent from my iPhone From carl.hobbs at kcl.ac.uk Sun Jan 17 14:18:42 2016 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sun, 17 Jan 2016 20:18:42 +0000 Subject: [Histonet] What has happened to Histonet Images site? Message-ID: Curiously, Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From LRaff at uropartners.com Mon Jan 18 07:35:07 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 18 Jan 2016 13:35:07 +0000 Subject: [Histonet] Floor Choice Question, and blog post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B62622C@COLOEXCH01.uropartners.local> Good Morning from Frigid Chicago: Our lab has the opportunity to do some badly needed painting, and also to make changes in the floor surfaces. We currently have vinyl in the clinical areas and carpeting in the offices. The carpeting will stay. Our decision is whether to swap out the vinyl or not. It is slippery, particularly for visitors coming from outside in the snow and sleet. We are working with the building management firm, but as of yet have not found a surface that is less slippery, but also is smooth enough to allow the maintenance people to scrape paraffin of the floor. Does anyone have any good flooring suggestions? Also, what is the longest anyone has closed a lab for maintenance work? In the vein of remodeling, a new blog post: http://www.chicagonow.com/downsize-maybe/2016/01/isnt-this-blog-supposed-to-be-about-building-a-house/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From DKBoyd at chs.net Mon Jan 18 08:20:03 2016 From: DKBoyd at chs.net (Boyd, Debbie M) Date: Mon, 18 Jan 2016 14:20:03 +0000 Subject: [Histonet] Floor Choice Question, and blog post In-Reply-To: <6347C6D2B080534F9B5C2B08436DCFAF0B62622C@COLOEXCH01.uropartners.local> References: <6347C6D2B080534F9B5C2B08436DCFAF0B62622C@COLOEXCH01.uropartners.local> Message-ID: <7EAFE982E328304DA6CE2B677BB76246AB4ACCA3@TN001WEXMBX014.US.chs.net> There is a non-slip epoxy paint that is very durable. There is also a textured flooring that is used in both histology labs and OR suites. Neither is cheap but a lot less than legal repercussions. We have the textured flooring in our lab. Works great. A little harder to scrub, EVS uses a buffer and of course no wax. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Lester Raff MD via Histonet [histonet at lists.utsouthwestern.edu] Sent: Monday, January 18, 2016 8:35 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [EXTERNAL] [Histonet] Floor Choice Question, and blog post Good Morning from Frigid Chicago: Our lab has the opportunity to do some badly needed painting, and also to make changes in the floor surfaces. We currently have vinyl in the clinical areas and carpeting in the offices. The carpeting will stay. Our decision is whether to swap out the vinyl or not. It is slippery, particularly for visitors coming from outside in the snow and sleet. We are working with the building management firm, but as of yet have not found a surface that is less slippery, but also is smooth enough to allow the maintenance people to scrape paraffin of the floor. Does anyone have any good flooring suggestions? Also, what is the longest anyone has closed a lab for maintenance work? In the vein of remodeling, a new blog post: http://www.chicagonow.com/downsize-maybe/2016/01/isnt-this-blog-supposed-to-be-about-building-a-house/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From relia1 at earthlink.net Mon Jan 18 09:48:35 2016 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 18 Jan 2016 10:48:35 -0500 Subject: [Histonet] RELIA Histology Careers Bulletin 1/18/2016 What does 2016 Have in store for you? Message-ID: <013a01d15207$b0e213f0$12a63bd0$@earthlink.net> Hi Histonetters! What does 2016 have in store for YOU? What Are You Going To Do With This Brand Spanking Shiny New Year? Is there something to check off your bucket list? Someone you need to get back in touch with? A Book you have been meaning to read? A class you have been meaning to take? Could the time be right for you to explore a job change or new Career Opportunities? What kind of change would interest you? A move to a new city? A different type of lab environment? A different schedule? More Money? Better Benefits? A Promotion? I have a nationwide network of contacts with the premier employers of histology professionals I only work with full time permanent positions at companies that offer excellent compensation, benefits programs and in most cases relocation assistance and or sign on bonuses. Here is a list of my current openings: Histology Management: Histology Lab Supervisor - Modesto, CA Pathology Manager - Manchester, NH Dermpath Histology Supervisor - Fayetteville, AR Histology Technician/Technologist: Histology Tech - Norfolk, VA 2nd 3rd and rotating shifts 15K sign on bonus!!! Clinical Lab Specialist - Norfolk, VA 1st shift 15K sign on bonus BS req. Histotechnician - Modesto, CA Lead Dermpath Histotech - Fayetteville, AR Dermpath Histotech Kansas City, KS Histotech - Little Rock, AR Mohs Tech - Sun City, FL Histotech DAYS - Milwaukee, WI Dermpath Histotech - Tyler, TX So if any of these positions look interesting or you or anyone you know is interested in making a job change this year please let me know. We can get started right away or we can map out a strategy for when the timing is right. Shoot me an e-mail at relia1 at earthlink.net or call me toll free at 866-607-3542. There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 12 years I have dedicated my practice solely to placing histology professionals like you. Happy New Year and I hope to hear from you soon. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thanks-Pam Happy New Year! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From melissa at alliedsearchpartners.com Mon Jan 18 10:36:03 2016 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Mon, 18 Jan 2016 16:36:03 +0000 Subject: [Histonet] Dermatology Histotech Job in Aurora, CO Message-ID: In search of a Histotech with experience working at a Dermatologists office. The position is Monday-Friday Day Shift. Please contact me for more details. Thank you! To view a complete list of Allied Search Partners current openings go to: http://www.jobs.net/jobs/alliedsearchpartners/en-us/all-jobs/United-States/ Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 From garreyf at gmail.com Mon Jan 18 10:53:37 2016 From: garreyf at gmail.com (Garrey Faller) Date: Mon, 18 Jan 2016 11:53:37 -0500 Subject: [Histonet] policy for return of specimens to patients (hardware, gallstones, placentas, etc) Message-ID: <58ED509B-A104-4B0B-998F-5CA6D640669B@gmail.com> HI everyone, Are any of you willing to share your policy? It is not uncommon for a patient to request his/her gallbladder, stone, placentas, hardware. Patients also rarely request their placentas to eat. I believe patients to have rights to their tissues. Thanks in advance. Garrey From Cwagner at samaritanhospital.org Mon Jan 18 13:10:44 2016 From: Cwagner at samaritanhospital.org (Candace J. Wagner) Date: Mon, 18 Jan 2016 19:10:44 +0000 Subject: [Histonet] Placenta Policies Message-ID: <1005CFAD0A252C4894B839AECE87A3F2012C89E488@SAM341.samaritanhospital.org> I too would be interested in anyone willing to share their policy on placenta exams or stillbirths? I'm in the process of updating ours and want to make sure I have all the current indications for doing a placental exam. Thank you. Candy ---------------------------------------------------------------------------------------------------------------- E-MAIL CONFIDENTIALITY NOTICE: The contents of this e-mail message and any attachments are intended solely for the addressee(s) and may contain confidential and/or legally privileged information. If you are not the intended recipient of this message or if this message has been addressed to you in error, please immediately alert the sender by reply e-mail and then delete this message and any attachments. If you are not the intended recipient, you are notified that any use, dissemination, distribution, copying, or storage of this message or any attachment is strictly prohibited. From liz at premierlab.com Mon Jan 18 14:16:22 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Mon, 18 Jan 2016 13:16:22 -0700 Subject: [Histonet] NSH Awards and Scholarships Message-ID: <14E2C6176416974295479C64A11CB9AE02854EF540D2@SBS2K8.premierlab.local> Hello Histonetters It's that time of the year again. Nominations for NSH Awards and Scholarships is open. Histotechs do great work every day. What better way to honor those leaders in the field than by nominating them for an NSH award? Check out all of the awards offered by NSH and nominate that deserving individual. Liz NSH Awards Chairperson Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From lindamargraf at gmail.com Mon Jan 18 16:33:37 2016 From: lindamargraf at gmail.com (Linda Margraf) Date: Mon, 18 Jan 2016 16:33:37 -0600 Subject: [Histonet] Fwd: Acetone and IHC References: <52B7E4673B4A6045988BC7065CA3B84815AF46CD@EXCH-2.tmmc.com> Message-ID: Sent from my iPhone Begin forwarded message: > From: "Dyer, Terry" > Date: January 18, 2016 at 11:59:14 AM CST > To: "'histonet-owner at lists.utsouthwestern.edu'" > Subject: Acetone and IHC > > I would appreciate input in reference to cytology button preparations and IHC staining. > Our laboratory commonly uses acetone and alcohol to make cytology buttons from body fluids. We often perform IHC on these > Cell blocks. Does anyone use acetone for cytology preparation? If so does this cause staining issues or hinder staining of the > Cell block? > Thank you, > Terry Dyer HT (ASCP) > Torrance Memorial Medical Center > 3330 Lomita Blvd. > Torrance, CA. 90505 > 310-325-9110 X1740 > From Timothy.Morken at ucsf.edu Tue Jan 19 10:27:16 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 19 Jan 2016 16:27:16 +0000 Subject: [Histonet] Al Floyd, a great loss to NSH Message-ID: <761E2B5697F795489C8710BCC72141FF6FD0A339@ex07.net.ucsf.edu> For those that knew him or took courses from him, Al was a consummate professional. I was fortunate enough to work with him on some projects and found his knowledge and insight invaluable. He was also a good friend. He will be missed, especially as a fixture at NSH meetings. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center [http://ak-cache.legacy.net/legacy/images/cobrands/southbendtribune/photos/0020265377-01-1_20151101.jpg?v=0x00000000330702d6] Alton David Floyd July 17, 1941 - Oct. 29, 2015 EDWARDSBURG - Alton D. Floyd, a true renaissance man, passed away Thursday, October 29, 2015. He was born to the late Frank and Queen Tina (Melton) in Henderson, Kentucky, on July 17, 1941. He was a Kentucky farm boy who excelled in 4-H and played trumpet in high school and the University of Kentucky marching band. Upon graduation from the University of Kentucky, Alton earned a Ph.D. from the University of Louisville, followed by six years on the faculty at the University of Michigan Medical School and thirteen years at Indiana University Medical School. He married the love of his life, Barbara Wilson, with whom he celebrated 53 years of marriage this past July. Surviving are his wife Barbara of Edwardsburg, Michigan, and their two daughters, Fara McCune and her husband, Michael, and their children, Liam, Finnian and Fiona; and Heather Floyd and her husband, Dan Willems; as well as his sister, Brenda Herron. The last 30 years of Alton's career were spent as a consultant in the pharmaceutical and medical diagnostic industries. He derived great satisfaction from teaching many years at the annual National Society for Histotechnology. He also served on the Board of Directors for several organizations. Alton also loved music, especially Jazz. He valued his membership in the Michiana Amateur Radio Club where he was known by the call sign W8ADF, and attending their Tuesday afternoon luncheons. Retirement gave him the opportunity to enjoy many artistic pursuits: designing and creating jewelry, participating in classes at the Krasl Art Museum, and woodworking - from intricate carvings to making furniture, painting and stained glass. He especially enjoyed lake activities each summer with his grandchildren. Alton passed the same way he lived, with extreme grace and dignity. There will be no services at this time, but a celebration of his life is planned for next summer. Elkhart Cremation Service is assisting with the arrangements. - See more at: http://m.legacy.com/obituaries/southbendtribune/obituary.aspx?n=alton-floyd&pid=176297459&referrer=0&preview=false#sthash.IbF8q928.dpuf From tgenade at gmail.com Tue Jan 19 15:31:30 2016 From: tgenade at gmail.com (Tyrone Genade) Date: Tue, 19 Jan 2016 15:31:30 -0600 Subject: [Histonet] microtome issues: thick and no sections Message-ID: Hello, I am having a problem with sectioning. I'm cutting 5 um sections but with the turn of the microtome I will miss the block, or take a small piece of the block/tissue and then the next section ends up thick. This missing can take several turns of the wheel so I can get really big sections. I have checked the angle of the knife to the block and the tension along the blade but these are fine. I worry that the issue might be temperature. The microtome is on a bench against an outside wall. The temperature is about 15 oC. Could this be the issue? I am not cooling the block on ice. Previous experience with block-cooling was that I would get thick and thin sections that way... this is a very different problem. Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From latecor at adinet.com.uy Tue Jan 19 19:53:14 2016 From: latecor at adinet.com.uy (Carlos Defeo) Date: Wed, 20 Jan 2016 01:53:14 +0000 Subject: [Histonet] Thick and thin sections Message-ID: Tyrone: definitely no temperature issue in this case. Look if your using cassete that is correctly clamped, and then if the answer is no, check the other parts of the blade holder unit. If still no part seems to be loosen, you have to service the micrometric advance, what seems to be the most reasonable cause of the problem. Good luck and my kind regards, Carlos.- From j.benavides at eae.csic.es Wed Jan 20 07:15:30 2016 From: j.benavides at eae.csic.es (Julio Benavides) Date: Wed, 20 Jan 2016 14:15:30 +0100 Subject: [Histonet] Pinkerton's adaptation of machiavello's stain for chlamydophila on tissue sections In-Reply-To: References: Message-ID: <20160120141530.Horde._JPYgTK_UijYIziAB1JSyg1@webmail.csic.es> Hi there, I was wondering if any of you had used the Pinkerton's adaptation of machiavello's stain for staining chlamydophila on parafin tissue sections and whether it works or not. I would also be very grateful if anybody can advise in any other technique working on tissue sections. We are working with an ovine abortion here. Best regards, Julio benavides From badams at acadianagastro.com Wed Jan 20 12:21:00 2016 From: badams at acadianagastro.com (Brent Adams) Date: Wed, 20 Jan 2016 18:21:00 +0000 Subject: [Histonet] Histonet Digest, Vol 146, Issue 18 ( Microtome Thick and Thin issues) In-Reply-To: References: Message-ID: Tyrone I am in agreement with Carlos except low operating temperature can effect the lubrication of the slide mechanism by gelling up and causing the slide mechanism to skip and jump. Lubrication of the internal mechanics of the Microtome are very necessary on a regular basis. If volumes use is high then lubricants dry out causing skipping and jumping of the slide. Either way my recommendation is to have it Serviced right away and do not use any more until you have done so. Brent Adams ? BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________________ PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. From tgenade at gmail.com Wed Jan 20 17:10:59 2016 From: tgenade at gmail.com (Tyrone Genade) Date: Wed, 20 Jan 2016 17:10:59 -0600 Subject: [Histonet] microtome issues: thick and no sections (Tyrone Genade) Message-ID: Hello, Thanks to all who responded to my question. The solution, it seems was very simple. Several people reported similar experiences and issues with the specimen clamp not fitting tightly and that I should clean the clamp or block. My blocks are clean and I thought the clamp was too... but when I ran a set of forceps along the inner, upper edge of the clamp a tiny blob of wax was found. A test block cut normally. I'm amazed a tiny piece of wax could cause so much trouble! Thanks to everyone who offered help. Bye From ian.bernard at comcast.net Wed Jan 20 20:05:00 2016 From: ian.bernard at comcast.net (ian bernard) Date: Wed, 20 Jan 2016 19:05:00 -0700 Subject: [Histonet] Validation Process or Procedure for the Shandon Cytospin 4 and the Sorvall ST 8 Centrifuge Message-ID: <018001d153f0$228764d0$67962e70$@comcast.net> Does anyone have a validation protocol to share? Mr B From kiel.quartett at gmail.com Thu Jan 21 06:39:01 2016 From: kiel.quartett at gmail.com (Oliver Kiel) Date: Thu, 21 Jan 2016 13:39:01 +0100 Subject: [Histonet] Chromogen remover Message-ID: Dear Histonetties, our lab manager likes to use a chromogen remover for paraffin-embedded sections. I can?t find this product online. Maybe you know how we can prepare a chromogen remover? Thanks in advance for your advices. Oliver From pablo.sanchez at usc.es Thu Jan 21 06:52:04 2016 From: pablo.sanchez at usc.es (pablo.sanchez at usc.es) Date: Thu, 21 Jan 2016 13:52:04 +0100 Subject: [Histonet] Drivers for AxioCam MRc 5 from Zeiss Message-ID: <20160121135204.20563gs9getwi36s@correoweb.usc.es> Dear histonetters, I am looking for drivers for the AxioCam MRc 5 from Zeiss. Unfortunately Zeiss webpage is not very helpful. Thanks in advance Pablo From melissa at alliedsearchpartners.com Thu Jan 21 12:47:45 2016 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Thu, 21 Jan 2016 18:47:45 +0000 Subject: [Histonet] Cytotechnologist Job Opening- Message-ID: Hello, If there are any Cytotechs out there in histoland looking for a new opportunity please contact me if interested in the below. * Position: Cytotechnologist (Full Time Permanent) * Location: Michigan * Relocation Assistance, Interview Travel Reimbursement, Great Pay and Benefits! Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 From LRaff at uropartners.com Thu Jan 21 14:03:56 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 21 Jan 2016 20:03:56 +0000 Subject: [Histonet] Thanks...also blog post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B6396B2@COLOEXCH01.uropartners.local> http://www.chicagonow.com/downsize-maybe/2016/01/can-someone-create-this-app/ Thanks to the suggestions for flooring. We are finding the concept of moving all our block and slide cabinets to allow us to lay new flooring overwhelming. We may opt to have the housekeeping crew put on a new coating that supposedly is more slip resistant. WE are also still researching our disposal of 10 year old blocks. Thanks for the suggestions. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From bcooper at chla.usc.edu Thu Jan 21 14:35:37 2016 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Thu, 21 Jan 2016 20:35:37 +0000 Subject: [Histonet] Best Glass Coverslipper? Message-ID: Hey Histonet! What's the best automatic glass coverslipper out there? I only have experience with the Sakura tape slipper, which is AWESOME, but alas, we can't get one here. Space is definitely an issue; would love to hear your experiences! Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper at chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From mills at 3scan.com Thu Jan 21 18:56:33 2016 From: mills at 3scan.com (Caroline Miller) Date: Thu, 21 Jan 2016 16:56:33 -0800 Subject: [Histonet] Thanks...also blog post In-Reply-To: <6347C6D2B080534F9B5C2B08436DCFAF0B6396B2@COLOEXCH01.uropartners.local> References: <6347C6D2B080534F9B5C2B08436DCFAF0B6396B2@COLOEXCH01.uropartners.local> Message-ID: Thank you Lester, this is a great middle ground! As someone who was a little put out by your totally off-topic emails, I am totally OK with you promoting your blog post inside a legitimate histonet post. Good luck with your issues. yours, mills On Thu, Jan 21, 2016 at 12:03 PM, Lester Raff MD via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > http://www.chicagonow.com/downsize-maybe/2016/01/can-someone-create-this-app/ > > > Thanks to the suggestions for flooring. We are finding the concept of > moving all our block and slide cabinets to allow us to lay new flooring > overwhelming. We may opt to have the housekeeping crew put on a new coating > that supposedly is more slip resistant. WE are also still researching our > disposal of 10 year old blocks. Thanks for the suggestions. > > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From vapatpxs at yahoo.com Thu Jan 21 22:28:28 2016 From: vapatpxs at yahoo.com (Va Paula Sicurello) Date: Fri, 22 Jan 2016 04:28:28 +0000 (UTC) Subject: [Histonet] Results of My Histology Survey-Finally! References: <1798943113.8612194.1453436908438.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1798943113.8612194.1453436908438.JavaMail.yahoo@mail.yahoo.com> Well Listers, I finally get around to posting my survey. ?Only 35 people took it but it still provided useful information and I got an A in my class. Thank You to the 35 who participated All the data:? ? ? ? ? ? ?23 HT's, 8 HTL's, 4 with other certification (2 with HT/HTL) took my survey ? ? ? ? ? ? ?24 years of experience average. ? ? ? ? ? ? ? ????????????Range 5 to 45 years.? ? ? ? ? ? ?49 blocks per hour, average cutting. ? ? ? ? ????????????Range 19 to 120? ? ? ? ? ? ?69 slides per hour, average. ? ? ? ? ? ? ? ? ? ? ?????????????Range ?35 to 160/hour? ? ? ? ? ? ?7 average number of staff. ? ? ? ? ? ? ? ? ? ? ? ? ????????????Range 1 to 22 ? ? ? ? ? ? ?278 blocks per day per lab, average. ? ? ? ? ????????????Range 10 to 1000 ? ? ? ? ? ? ?467 H&E slides per day, per lab, average. ?????????????Range 10 to 1500 ? ? ? ? ? ? ? 92 IHC slides per day, per lab, average ? ? ?????????????Range 2 to 300 ? ? ? ? ? ? ?19 special stain slides per day, per lab, average ? ? Range 1 to 100 ?My take away from this was that we are getting older, we are working faster with fewer staff and we use automation to our advantage. I think none of this is surprising to you. ? ? Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health From rjbuesa at yahoo.com Fri Jan 22 08:03:46 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 22 Jan 2016 14:03:46 +0000 (UTC) Subject: [Histonet] Thanks...also blog post In-Reply-To: References: Message-ID: <590168759.7336450.1453471426866.JavaMail.yahoo@mail.yahoo.com> Long live the subterfuge! Ren? On Thursday, January 21, 2016 8:04 PM, Caroline Miller via Histonet wrote: Thank you Lester, this is a great middle ground! As someone who was a little put out by your totally off-topic emails, I am totally OK with you promoting your blog post inside a legitimate histonet post. Good luck with your issues. yours, mills On Thu, Jan 21, 2016 at 12:03 PM, Lester Raff MD via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > http://www.chicagonow.com/downsize-maybe/2016/01/can-someone-create-this-app/ > > > Thanks to the suggestions for flooring. We are finding the concept of > moving all our block and slide cabinets to allow us to lay new flooring > overwhelming. We may opt to have the housekeeping crew put on a new coating > that supposedly is more slip resistant. WE are also still researching our > disposal of 10 year old blocks. Thanks for the suggestions. > > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From emcca2 at gmail.com Fri Jan 22 09:00:17 2016 From: emcca2 at gmail.com (Erin McCarthy) Date: Fri, 22 Jan 2016 09:00:17 -0600 Subject: [Histonet] HPV DNA Probe question Message-ID: I am curious what people are using for HPV probes. We recently stumbled upon the fact that our current probe choice is RUO - to avoid a sticky validation and LDT process I would like to replace that with an ASR or IVD ideally. I cannot seem to find one, however. Additionally, our Pathologists prefer HR and LR be run on separate slides instead of as a cocktail. Anyone have any thoughts? *Erin McCarthy HT, ASCP* Third Shift Lead Histotechnician OSF Saint Francis Medical Center 530 NE Glen Oak Ave. Peoria, IL Phone (309)624-9043 From melissa at alliedsearchpartners.com Fri Jan 22 10:11:10 2016 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Fri, 22 Jan 2016 16:11:10 +0000 Subject: [Histonet] Histotech Job Openings in Atlanta, GA- Message-ID: Hello Histoland, I have 2 openings for Full Time Histotechs in Atlanta, GA. The positions are Monday-Friday 11am-7pm. Please contact me if you are interested in learning more. Happy Friday! Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 From tbraud at holyredeemer.com Fri Jan 22 12:26:22 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 22 Jan 2016 18:26:22 +0000 Subject: [Histonet] best glass coverslipper Message-ID: <48E053DDF6CE074DB6A7414BA05403F805A92E@HRHEX02-HOS.holyredeemer.local> I've had experience with several glass coverslippers over the years. While I've not tried the newest/latest/greatest of other brands, I've been running the Sakura Glas coverslipper for 8 years without a hitch. It has been very dependable, with little downtime, and extremely easy maintenance. We have averaged less than 1 service call per year since purchase, outside of the annual pm. At the time we purchased it, it had the shortest set up and routine maintenance of the 4 big players that we tried. It is definitely a more sensitive system than the tape. All glass systems are less forgiving of technical error than tape, but the results are fabulous. We coverslip using only 24 X 50, so avoid the hassles of changing sizes. I hope this will help. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 3. Best Glass Coverslipper? (Cooper, Brian) Message: 3 Date: Thu, 21 Jan 2016 20:35:37 +0000 From: "Cooper, Brian" Hey Histonet! What's the best automatic glass coverslipper out there? I only have experience with the Sakura tape slipper, which is AWESOME, but alas, we can't get one here. Space is definitely an issue; would love to hear your experiences! Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper at chla.usc.edu From liz at premierlab.com Fri Jan 22 13:25:59 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Fri, 22 Jan 2016 12:25:59 -0700 Subject: [Histonet] best glass coverslipper In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F805A92E@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F805A92E@HRHEX02-HOS.holyredeemer.local> Message-ID: <14E2C6176416974295479C64A11CB9AE02854EF5410B@SBS2K8.premierlab.local> I would second Terri's comments on the Sakura Glas coverslipper. I do have to stress that yearly PM's are very important and that there is daily maintenance that needs to be done in order for the instrument to function properly, we also only use 24x50's. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Terri Braud via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, January 22, 2016 11:26 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] best glass coverslipper I've had experience with several glass coverslippers over the years. While I've not tried the newest/latest/greatest of other brands, I've been running the Sakura Glas coverslipper for 8 years without a hitch. It has been very dependable, with little downtime, and extremely easy maintenance. We have averaged less than 1 service call per year since purchase, outside of the annual pm. At the time we purchased it, it had the shortest set up and routine maintenance of the 4 big players that we tried. It is definitely a more sensitive system than the tape. All glass systems are less forgiving of technical error than tape, but the results are fabulous. We coverslip using only 24 X 50, so avoid the hassles of changing sizes. I hope this will help. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 3. Best Glass Coverslipper? (Cooper, Brian) Message: 3 Date: Thu, 21 Jan 2016 20:35:37 +0000 From: "Cooper, Brian" Hey Histonet! What's the best automatic glass coverslipper out there? I only have experience with the Sakura tape slipper, which is AWESOME, but alas, we can't get one here. Space is definitely an issue; would love to hear your experiences! Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper at chla.usc.edu _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From steven.weston at utas.edu.au Sat Jan 23 19:17:52 2016 From: steven.weston at utas.edu.au (Steven Weston) Date: Sun, 24 Jan 2016 01:17:52 +0000 Subject: [Histonet] re best glass coverslipper Message-ID: <1453598271721.42634@utas.edu.au> I've been using the Dako coverslipper for over three years now. Once i had got used to ensuring the sliding mechanism was always kept mountant free and regularly changed the xylene in the u tube we have had very few problems with this machine. Its footprint is without question the smallest coverslipper i have seen and generally with good maintenance (yearly PM) it functions very well. Occasionally we get small bubbles under the coverslips but they are easily removed. Highly recommend looking at one, also the price is very good. regards steve weston lab manager Breathe-Well CRE UTAS-SOM University of Tasmania Electronic Communications Policy (December, 2014). This email is confidential, and is for the intended recipient only. Access, disclosure, copying, distribution, or reliance on any of it by anyone outside the intended recipient organisation is prohibited and may be a criminal offence. Please delete if obtained in error and email confirmation to the sender. The views expressed in this email are not necessarily the views of the University of Tasmania, unless clearly intended otherwise. From emartinez2 at echd.org Mon Jan 25 11:55:56 2016 From: emartinez2 at echd.org (Estela Martinez) Date: Mon, 25 Jan 2016 17:55:56 +0000 Subject: [Histonet] PAX8 ANTIBODY Message-ID: Hello All, Can some recommend me a good clone on PAX8 and who you get it from. Thank You in advance!! Estela Martinez Histology Supervisor Medical Center Hospital Odessa, TX 79761 432-640-2348 emartinez2 at echd.org CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party without permission of original user and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From bcooper at chla.usc.edu Mon Jan 25 13:36:52 2016 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Mon, 25 Jan 2016 19:36:52 +0000 Subject: [Histonet] Best Glass Coverslipper? In-Reply-To: References: Message-ID: Thank you to all who responded to my inquiry about the best glass coverslipper! Everyone loves their Leica CV5030's and Sakura Glas G2's! Thanks, Brian -----Original Message----- From: Cooper, Brian via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, January 21, 2016 2:36 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Best Glass Coverslipper? Hey Histonet! What's the best automatic glass coverslipper out there? I only have experience with the Sakura tape slipper, which is AWESOME, but alas, we can't get one here. Space is definitely an issue; would love to hear your experiences! Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper at chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From vincentroy105 at live.fr Mon Jan 25 17:59:48 2016 From: vincentroy105 at live.fr (Vincent Roy) Date: Mon, 25 Jan 2016 18:59:48 -0500 Subject: [Histonet] histonet@lists.utsouthwestern.edu Message-ID: Hello everyone from Histonet! I am a student in Master?s Degree at the Universit? du Qu?bec ? Rimouski, Qu?bec, Canada. I am currently trying to get striated muscle information from Onchorhynchus mykiss, a fish from our place, with histology means. I do that every year to help other students through a synthesis project. Last year, the histology was wonderful and results were interesting. This year, we try again with the same specimens, with the same manipulations but something odd occurs (see pictures ?Bad sections of O Mykiss?). The cells are damaged and the look like crags in a desert. The nuclei aren?t visible, and I can?t get information from striated muscles based on those. Those pictures are from specimens from last year, which gave great slides last year (pictured available named ?Bad sections of O Mykiss? So here?s my whole walkthrough this year : * The specimens are conserved in 90% Ethanol. Those were cut so that we only work on the parts that interests us. * The specimens were processed with Thermo Shandon Citadel 2000 machinery with the standard protocol (Formalin 2h, Formalin 2h, Ethanol 70%, Ethanol 90%, Ethanol 100% x3, Xylene x3 and Paraffin x2 for a total of 20h) o Right after that step, the specimens were tinted pink. We figured out that would be the problem for the bad slices, but it?s not since I tried coloration on older ?already embedded? specimens that worked last year, conserved in refrigerator. Anyway, the pink tint is probably from the old recycled xylene that probably went in contact with eosin, so we changed it. Currently testing. * The specimens were embedded using Thermo Shandon Histocentre. After that, they were stored in refrigerator for later use. * The specimens were cut using Thermo Shandon Finesse Microtome. The slices are 8 micrometers thick and represent a transversal cut of the fish. * The specimens were ?cooked? on a slide warmer from Fisher Scientific at 50-60 degrees for 10-20 minutes, depending on the paraffin melting level. The goal was to mechanically fix the whole specimen on the slide so it doesn?t fall during coloration, which worked. o I do not use warm bath since we don?t have one equipment for that, but it would be nice. * The specimens were colored using this protocol : o 2 min in Xylene o 2 min in Xylene o 2 min in Xylene o 2 min in 100% OH o 2 min in 100% OH o 1 min in 95% OH o 1 min in 70% OH o 2 min in distilled water o 5 min in hematoxylin (recycled/used or not, makes the same results) o 3 min in circulating water o 1 min in a differentiation solution o 30 secs in distilled water o 1 min in bluing solution, consisting of 0,2g of sodium bicarbonate in 100ml of distilled water o 3 min in distilled water o 25 secs in Eosin (recycled/used or not, makes the same results) o 15 secs in 95% OH o 30 secs in 100% OH o 1 min in 100% OH o 2 min in 100% OH o 2 min in Xylene o 2 min in Xylene o 2 min in Xylene * ***The coloration testing this year was made manually with little cups and chronometer, because we can?t afford to use quantities of product in the ?Varistain? machinery if they are not even good. * The specimens were then observed in microscopy, and here we are. I do not have any clues why the cells look so damaged. I was hoping for you guys to have a good answer. I am not a histologist, nor I am the most familiar with all the techniques. My protocol is mostly based on Humason?s Animal Tissue Techniques ! Thank you for the help! Vincent Roy Candidat ? la ma?trise en gestion de la faune et de ses habitats Laboratoire de Pal?ontologie et de Biologie ?volutive Universit? du Qu?bec ? Rimouski 300 All?e des Ursulines Rimouski, QC G5L 3A1 From LRaff at uropartners.com Tue Jan 26 07:44:14 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 26 Jan 2016 13:44:14 +0000 Subject: [Histonet] Tissue FISH and post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B65AA6E@COLOEXCH01.uropartners.local> Good Morning: In labs that do tissue FISH, does the histology lab do the entire FISH process, or cut the slides and then pass off to the molecular department? Thanks, New post: http://www.chicagonow.com/downsize-maybe/2016/01/i-have-a-trophy-wife-and-other-things-i-learned-playing-tennis-twice-a-week/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From DShank at chpnet.org Tue Jan 26 08:14:08 2016 From: DShank at chpnet.org (Deborah Shank) Date: Tue, 26 Jan 2016 09:14:08 -0500 Subject: [Histonet] Tissue FISH and post In-Reply-To: <6347C6D2B080534F9B5C2B08436DCFAF0B65AA6E@COLOEXCH01.uropartners.local> References: <6347C6D2B080534F9B5C2B08436DCFAF0B65AA6E@COLOEXCH01.uropartners.local> Message-ID: <603B84B39988864E9E4E9F24812B3086D5A71CC66D@CHPMAILVS7.AD.CHPNET.ORG> At MSBIMC the Histology lab cuts the tissue, but FISH is performed by the Immunpathology lab. We also do all the IHC, Flow Cytometry, and Clinical Immunology testing. ________________________________________ From: Lester Raff MD via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, January 26, 2016 8:44 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Tissue FISH and post Good Morning: In labs that do tissue FISH, does the histology lab do the entire FISH process, or cut the slides and then pass off to the molecular department? Thanks, New post: http://www.chicagonow.com/downsize-maybe/2016/01/i-have-a-trophy-wife-and-other-things-i-learned-playing-tennis-twice-a-week/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and any attachments are confidential and intended solely for the use of the individual or entity to which they are addressed. If you are not the intended recipient, you are prohibited from printing, copying, forwarding, saving, or otherwise using or relying upon them in any manner. Please notify the sender immediately if you have received this message by mistake and delete it from your system. From k84as at yahoo.com Tue Jan 26 13:32:37 2016 From: k84as at yahoo.com (mohamed abd el razik) Date: Tue, 26 Jan 2016 19:32:37 +0000 (UTC) Subject: [Histonet] unsolved proplem References: <1161621322.506108.1453836757971.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1161621322.506108.1453836757971.JavaMail.yahoo@mail.yahoo.com> Dear allI have submitted a proplem about reprocessing tissue blocks that have patches of stained areas and unstained pale yellow areas (H&E stain)!!!, unfortently the proplem didn't solved yet and i have tried all possible solutions, increasing processing time and ordered new chemicals from other soruces and changed the paraplast company but still have the proplem !!!! i'm using 70% alc 2hr - 80% alc 1hr - 90% alc 45mins- absolut 1 and 2 each for 30 mins then Benzen 1 and 2 each for 25 mins then clearing by methyl benzoat 2hs at least then paraffin1+2 (Maccormick melting degree 56-58)each 90 mins for mice kidney, liver and tests the previous protocol was very satisfactory to us and we have tried to stain older sections processed befor the emerging proplem and have a nice stain by current H&E stain to ensure that the proplem is out our staining step. any suggestions please !! Mohamed From JMacDonald at mtsac.edu Tue Jan 26 13:36:33 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Tue, 26 Jan 2016 11:36:33 -0800 Subject: [Histonet] unsolved proplem In-Reply-To: <1161621322.506108.1453836757971.JavaMail.yahoo@mail.yahoo.com> References: <1161621322.506108.1453836757971.JavaMail.yahoo.ref@mail.yahoo.com> <1161621322.506108.1453836757971.JavaMail.yahoo@mail.yahoo.com> Message-ID: There are a couple of things that it might be. 1. Uneven deparaffinization before staining. 2. Water in your last reagents/paraffin on the processor. From: mohamed abd el razik via Histonet To: "Histonet at lists.utsouthwestern.edu" Date: 01/26/2016 11:33 AM Subject: [Histonet] unsolved proplem Dear allI have submitted a proplem about reprocessing tissue blocks that have patches of stained areas and unstained pale yellow areas (H&E stain)!!!, unfortently the proplem didn't solved yet and i have tried all possible solutions, increasing processing time and ordered new chemicals from other soruces and changed the paraplast company but still have the proplem !!!! i'm using 70% alc 2hr - 80% alc 1hr - 90% alc 45mins- absolut 1 and 2 each for 30 mins then Benzen 1 and 2 each for 25 mins then clearing by methyl benzoat 2hs at least then paraffin1+2 (Maccormick melting degree 56-58)each 90 mins for mice kidney, liver and tests the previous protocol was very satisfactory to us and we have tried to stain older sections processed befor the emerging proplem and have a nice stain by current H&E stain to ensure that the proplem is out our staining step. any suggestions please !! Mohamed _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting at geisinger.edu Tue Jan 26 14:43:46 2016 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Tue, 26 Jan 2016 20:43:46 +0000 Subject: [Histonet] NHPD Message-ID: Is it me or does anyone else think $20 is a lot of money for a T-shirt? The Histotechnology Professionals Day T-shirts on NSH'S website seem over-priced. Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From cforster at umn.edu Tue Jan 26 14:53:37 2016 From: cforster at umn.edu (Colleen Forster) Date: Tue, 26 Jan 2016 14:53:37 -0600 Subject: [Histonet] NHPD In-Reply-To: References: Message-ID: I think that is about average for good Tshirts these days. C On Tue, Jan 26, 2016 at 2:43 PM, Bitting, Angela K. via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Is it me or does anyone else think $20 is a lot of money for a T-shirt? > The Histotechnology Professionals Day T-shirts on NSH'S website seem > over-priced. > > Angie > > > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. It > is intended solely for the addressee. Access to this message by anyone else > is unauthorized. If you are not the intended recipient, any disclosure, > copying, distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you have received this > message in error, please delete all electronic copies of this message (and > the documents attached to it, if any), destroy any hard copies you may have > created and notify me immediately by replying to this email. Thank you. > > Geisinger Health System utilizes an encryption process to safeguard > Protected Health Information and other confidential data contained in > external e-mail messages. If email is encrypted, the recipient will receive > an e-mail instructing them to sign on to the Geisinger Health System Secure > E-mail Message Center to retrieve the encrypted e-mail. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jshelley at sbpdiscovery.org Wed Jan 27 08:15:05 2016 From: jshelley at sbpdiscovery.org (John Shelley) Date: Wed, 27 Jan 2016 14:15:05 +0000 Subject: [Histonet] Tissue Arrays Message-ID: Good Morning, I have purchased some arrays from a company in the past and had some very varying results. I prefer not to mention the company and so my request is if those of you who do not have the luxury of having tissue at your disposal to make your own tissue arrays from whom are you buying them from. Once purchased have you used them on IHC platforms like Ventana Benchmark/Ultra or Leica Bond3? Were your results consistent across the slide? I would like to add that I am looking for neuroblastoma tumor arrays. Any help will be greatly appreciated. Thanks in advance! Kind Regards! John J Shelley From jvalentine-williams at neogenomics.com Wed Jan 27 12:37:52 2016 From: jvalentine-williams at neogenomics.com (Jennifer Valentine-Williams) Date: Wed, 27 Jan 2016 18:37:52 +0000 Subject: [Histonet] EWSR1 FISH positive tissue request Message-ID: <8af18fada93b464dba1ef109b34b52e3@tp1pvm-mbx1.neogen.local> Hello Histoland, I'm reaching out in hopes that someone may have some unstained slides of Ewing Sarcoma FFPE they can share with me. I'm working on a validation and it had proved to be a difficult task in finding a positive case for FISH. If you have anything that you could share, I can provide you with our FedEx account for shipping. Thanks in advance! Jennifer Valentine-Williams, HT(ASCP) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secured or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. NeoGenomics Laboratories, Suite 5, 12701 Commonwealth Dr, Fort Myers, FL 33913, http://www.neogenomics.com (2013) From dtp110020 at utdallas.edu Wed Jan 27 12:41:21 2016 From: dtp110020 at utdallas.edu (Pruitt, David) Date: Wed, 27 Jan 2016 18:41:21 +0000 Subject: [Histonet] Issue with cresyl violet staining of rat brain sections Message-ID: Hey all, We have been having some issues with sectioning and staining rat brain tissue. We are doing doing a simple nissl + myelin stain. Here are all the details: (1) Rats are perfused with 4% paraformaldehyde. After perfusion, brains are post-fixed in PFA for at least 24 hours, but some of the brains have been sitting in PFA for up to 1 year. Brains are then transferred to a 30% sucrose solution, and allowed to sit in sucrose for at least 72 hours (although some brains have been sitting in sucrose for up to 1 year). (2) Brains are removed from the sucrose solution, and the first 10 mm of brain tissue is "blocked". The whole brain is embedded in TissueTEK embedding medium (we also have Shandon M-1 available, but have typically used TissueTEK). The brain is sectioned in a cryostat set to -21 C. Tissue sections are 40 um thick. We save every tissue section from the 10 mm block of tissue. (3) For mounting tissue, we have tried 2 different techniques. The first method is to take each tissue section and store it in a small well of 0.01 M PBS until we are ready to mount the tissue on slides (typically less than a week, often only 1 or 2 days). The other technique is to "direct mount" tissue in the cryostat by lowering a slide down to the stage and "catching" the tissue on to the slide. Often times, when we use this second techique, we wet the slide with a little bit of PBS because I have found it allows the tissue to "catch" easier, and as far as I can tell it helps eliminate bubbles in the tissue. I will refer to these two techniques as "wet mounting" and "direct mounting". "Wet mounted" slides are mounted using a dish of PBS and a paintbrush. (4) Tissue sections are mounted on SuperFrost Plus slides. No gelatin needed. (5) After mounting, slides are typically allowed to air-dry at room temperature for a day or two before staining. Occasionally staining happens on the same day as mounting. Also, occasionally, staining happens up to a week after mounting. (6) The staining protocol is thus: 2 min dH2O - hydrate/rinse tissue 2 min 70% EtOH - dehydrate tissue to prepare for clearing 2 min 95% EtOH - same as above 2 min 100% EtOH - same as above 5 min Citrisolv - clear the tissue (we use Citrisolv instead of Xylene) 2 min 100% EtOH - rehydrate tissue to prepare for staining 2 min 95% EtOH - same as above 2 min 70% EtOH - same as above 2 min dH2O - same as above 10 min Myelin stain (we use a Cyanine R-based myelin stain, pH is 2.0) 1.5 min differentiation step (in an ammonium hydroxide solution) 2 min dH2O 20 min Cresyl Violet stain (0.1% cresyl violet acetate solution, pH is 4.5) 2 min dH2O 5 seconds 70% EtOH (dehydrate tissue for clearing) 5 seconds 95% EtOH (same as above) 5 seconds 100% EtOH (same as above) 3 min Citrisolv (clear tissue in preparation for coverslipping) (7) Coverslipping - We coverslip using DPX as our coverslipping medium. We do not allow the slides to dry before coverslipping. We remove them directly from the Citrisolv and coverslip immediately. So, now that I have described our methods thoroughly, here is the problem: We are getting a lot of horizontal cracking (primarily during the sectioning process), and a lot of curling and sections falling off of slides (during the staining process). We have tried quite a bit to alleviate the problem, but not much seems to work. It seems like the tissue sections falling off during the staining process may have been due to the direct mounting technique - or so we think. The explanation we have come up with is that the embedding medium was getting inbetween the slide and the tissue, and then causing the tissue to fall off during the staining process. Is this logical? We have stopped direct mounting, and that problem seems to have stopped - hopefully. We still have a lot of curling of tissue during staining, and cracking of tissue during sectioning, however. Cracking usually happens near the caudal/rear portion of the 10 mm block of brain (when we approach the hippocampus), but rarely towards the anterior portion of the brain (motor cortex). Lately we have attempted sectioning at warmer temperatures (-16 C) as well as tried embedding only the base of the brain instead of the whole brain. Results are mixed so far. Also, nothing seems to have helped the curling issue during staining. I have uploaded 6 sample brain sections at this Google Photos link: https://goo.gl/photos/ATQQDwq6Whs8JX8J8 I am also going to try uploading them to the Histonet Images server. This is my first post to this listserv, so hopefully it works! Thanks for any advice and comments. We are really stumped as to how to solve the problem. - David From KSimeone at leavittmgt.com Wed Jan 27 12:41:58 2016 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Wed, 27 Jan 2016 18:41:58 +0000 Subject: [Histonet] Laboratory clerical job position *Delray Beach, FL* Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CB902C3B2@vm-email.leavittmgt.com> Hi Histonetters. I thought maybe I'd share a position we have available in our dermatology laboratory that is NOT TECHNICAL. Maybe you can share with someone who might be looking for a job in our area of Delray Beach, Florida. It is M-F 9-5p, FULL TIME clerical work. 401k, benefits and vacation. 25k annually. Filing, phones, doctor interaction/assisting and data entry. Must take drug and personality tests along with a background check. No experience necessary, WILL TRAIN. Please click on link to apply. https://advancedderm.applicantpro.com/jobs/242606.html Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor Delray Beach Technical Laboratory ADCS Clinics, LLC www.advancedderm.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From badams at acadianagastro.com Wed Jan 27 13:29:29 2016 From: badams at acadianagastro.com (Brent Adams) Date: Wed, 27 Jan 2016 19:29:29 +0000 Subject: [Histonet] Alliance Rubber Bands Message-ID: Hey Histonetters, I don't endorse products but this one is really good. I don't get a kick back and no relatives work for the company, I just wanted to give you all a good product to use if you are looking. I looked up the company and found they are the last U.S. Made Rubber Band company and based out of Arkansas. I think I have had 2 eye injuries and multiple painful mishaps from rubber bands that break far to easily so from that perspective I feel I can give this product a thumbs up. For the last 4 years I have been using Alliance Rubber Company non- latex rubber bands. The size 64 (3.5 x 1/4) work best on Slide folders I have found. The Orange color rubber bands are easy to see and hold the file folders together great and they rarely break. I know it sound silly to talk about rubber bands this way but I was really getting tired of being snapped by faulty rubber bands when trying to wrap slide folders or paperwork. I just had my first rubber band by Alliance snap a couple months ago which caught me off guard cause it had been so long since that happened. It was then I look to see what brand we were using and it turned out that we had been using the same NON-latex rubber bands from the Alliance Rubber Company since I opened the Laboratory in 2012. Before that at other labs breaking rubber bands were just part of the daily Job. I think purchasing has gotten their best price on Amazon but they also use staples and Office depot for supplies. I informed them that I only want these rubber bands and they can get their best pricing where ever. My fingers hurt a lot less after distributing slide folders and I don't have to swear as much. Ha Ha. Funny how little things can really bother us. FYI Brent Adams - BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. From melissa at alliedsearchpartners.com Wed Jan 27 13:35:19 2016 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Wed, 27 Jan 2016 19:35:19 +0000 Subject: [Histonet] Part Time Histotech Job in West Chicago-Permanent Placement Message-ID: Hello Histoland, Just an quick note to say that I am currently looking for a qualified Histotech to work in a part time position for the evening shift on a permanent basis. This position is in West Chicago, IL. Please contact me for more details. Have a great day! Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 From SHEILA.HERRINGTON at interiorhealth.ca Wed Jan 27 13:52:15 2016 From: SHEILA.HERRINGTON at interiorhealth.ca (HERRINGTON, SHEILA) Date: Wed, 27 Jan 2016 19:52:15 +0000 Subject: [Histonet] Microtomes Message-ID: <1FD5984EB6A52C4E857C54D9A9D38F3A20F2642D@IHSERV0018.interiorhealth.ca> We are looking to purchase two new microtomes and were wondering on thoughts and recommendations from the experience of the people that actually use them. Pros and cons would be extremely valuable on ergonomics, reliability and overall performance. Sheila Herrington, RT Technical Lead, Immunohistochemistry and Histopathology Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 x 7510 Sheila.herrington at interiorhealth.ca From bcooper at chla.usc.edu Wed Jan 27 15:32:39 2016 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 27 Jan 2016 21:32:39 +0000 Subject: [Histonet] Alliance Rubber Bands In-Reply-To: References: Message-ID: Awesome post Brent! I thought I was the only one who had a strong opinion about rubber bands! I never used the orange color bands you mentioned, but the Alliance Pale Crepe Gold #64 were pretty awesome too. Agree totally--cheap rubber bands HURT!!!! Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper at chla.usc.edu -----Original Message----- From: Brent Adams via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, January 27, 2016 11:29 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Alliance Rubber Bands Hey Histonetters, I don't endorse products but this one is really good. I don't get a kick back and no relatives work for the company, I just wanted to give you all a good product to use if you are looking. I looked up the company and found they are the last U.S. Made Rubber Band company and based out of Arkansas. I think I have had 2 eye injuries and multiple painful mishaps from rubber bands that break far to easily so from that perspective I feel I can give this product a thumbs up. For the last 4 years I have been using Alliance Rubber Company non- latex rubber bands. The size 64 (3.5 x 1/4) work best on Slide folders I have found. The Orange color rubber bands are easy to see and hold the file folders together great and they rarely break. I know it sound silly to talk about rubber bands this way but I was really getting tired of being snapped by faulty rubber bands when trying to wrap slide folders or paperwork. I just had my first rubber band by Alliance snap a couple months ago which caught me off guard cause it had been so long since that happened. It was then I look to see what brand we were using and it turned out that we had been using the same NON-latex rubber bands from the Alliance Rubber Company since I opened the Laboratory in 2012. Before that at other labs breaking rubber bands were just part of the daily Job. I think purchasing has gotten their best price on Amazon but they also use staples and Office depot for supplies. I informed them that I only want these rubber bands and they can get their best pricing where ever. My fingers hurt a lot less after distributing slide folders and I don't have to swear as much. Ha Ha. Funny how little things can really bother us. FYI Brent Adams - BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From bcooper at chla.usc.edu Wed Jan 27 15:55:47 2016 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 27 Jan 2016 21:55:47 +0000 Subject: [Histonet] Microtomes In-Reply-To: <1FD5984EB6A52C4E857C54D9A9D38F3A20F2642D@IHSERV0018.interiorhealth.ca> References: <1FD5984EB6A52C4E857C54D9A9D38F3A20F2642D@IHSERV0018.interiorhealth.ca> Message-ID: This has been a Histonet post in the past; techs are fiercely loyal to their favorite brand and I'm sure you'll see people disagree with me as soon as I hit send! I prefer Microm. In our experience, block alignment is easier with the Microm's because the X and Y axis orientation knobs are positioned in such a manner that you can adjust block orientation with your left hand while your right hand is on the flywheel. This allows you to keep a constant sight line of the entire depth of the block face in relation to the knife. With the Leica microtomes, the positions of these screws are moved to the right, and the orientation of the Y axis requires you to either use two hands at once, or for you to move your left arm across your sight line to orient the Y axis. While certainly not insurmountable; this just makes block orientation take a little longer. Since we frequently have to recut blocks from different institutions, this is a big deal here. I'm sure the peeps from the other camp are going to say that it's all in the technique. To that I say, YEAH, YEAH, YEAH!!!! :) Both of these machines are well designed and will provide years and years of excellent service as long as they are well maintained. The best thing you can do is ask to demo both machines and see what you like best. Good luck! Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357???? Pager: 213-209-0184 bcooper at chla.usc.edu -----Original Message----- From: HERRINGTON, SHEILA via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, January 27, 2016 11:52 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Microtomes We are looking to purchase two new microtomes and were wondering on thoughts and recommendations from the experience of the people that actually use them. Pros and cons would be extremely valuable on ergonomics, reliability and overall performance. Sheila Herrington, RT Technical Lead, Immunohistochemistry and Histopathology Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 x 7510 Sheila.herrington at interiorhealth.ca _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From mwich at 7thwavelabs.com Wed Jan 27 16:24:11 2016 From: mwich at 7thwavelabs.com (Michele Wich) Date: Wed, 27 Jan 2016 22:24:11 +0000 Subject: [Histonet] ORO staining in pre- vs. post-fixed tissue Message-ID: <4ADAF16E8518764ABF9DCEB73129AA75233DE6EB@WAVE-EMAIL.7thwave.local> Does anyone know whether there is a difference, quantitatively, in Oil red O fat staining of tissue fixed in NBF prior to embedding in OCT vs. fresh frozen tissue embedded in OCT? I know that sections from fresh frozen tissues must be post-fixed in formalin before staining with Oil Red O. But is there any benefit to using this preparation over tissues that are fixed in formalin prior to OCT embedding (aside from sectioning/slide adhesion issues previously described)? Is the staining similar, or does one preparation give better staining results? Much gratitude for any advice you can give, Michele From amosbrooks at gmail.com Wed Jan 27 20:37:56 2016 From: amosbrooks at gmail.com (Amos Brooks) Date: Wed, 27 Jan 2016 21:37:56 -0500 Subject: [Histonet] Tissue Arrays Message-ID: Hi, My experience with microarrays is that they are sometimes a bit complicated to work with depending on the way they are constructed and the platform they are used on. Often these slides are dipped in paraffin to help preserve them. When this happens they need considerably longer deparaffinization. They also sometimes are not floated on a waterbath to transfer them to the slide but by a tape transfer process. The tape often interferes in IHC staining and especially on Ventana platforms that make use of the liquid coverslips. If you can try to get microarrays that are floated like regular sections, make sure you give them a good long soak in xylene, and perhaps increase the volume per slide of the antibodies. Best of luck, Amos On Wed, Jan 27, 2016 at 1:00 PM, wrote: > Message: 5 > Date: Wed, 27 Jan 2016 14:15:05 +0000 > From: John Shelley > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Tissue Arrays > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > > I have purchased some arrays from a company in the past and had some very > varying results. I prefer not to mention the company and so my request is > if those of you who do not have the luxury of having tissue at your > disposal to make your own tissue arrays from whom are you buying them from. > Once purchased have you used them on IHC platforms like Ventana > Benchmark/Ultra or Leica Bond3? Were your results consistent across the > slide? > > I would like to add that I am looking for neuroblastoma tumor arrays. Any > help will be greatly appreciated. Thanks in advance! > > Kind Regards! > > John J Shelley > From histologymarket at gmail.com Thu Jan 28 05:42:21 2016 From: histologymarket at gmail.com (Histology Market) Date: Thu, 28 Jan 2016 13:42:21 +0200 Subject: [Histonet] Klinipath Tape or Sakura Tape In-Reply-To: References: Message-ID: Does anyone use Klinipath Coverslipping Tape and Sakura Coverslipping Tape? Is there any issues comparing the Sakura Coverslipping Film? Best Regards From ENarvaez at Pathlinelabs.com Thu Jan 28 08:12:51 2016 From: ENarvaez at Pathlinelabs.com (Edison Narvaez) Date: Thu, 28 Jan 2016 14:12:51 +0000 Subject: [Histonet] SOX 10 Cellmarque Message-ID: <214af96e0c3c48c2973ae78186d2145c@RAMEXCHANGE.HPS.LOCAL> Hello, Anybody running Cellmarque's RTU SOX 10 antibody on the bond III, any protocol suggestions, I would like to run this antibody with Leica's red kit. Thank you ________________________________ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. From rjbuesa at yahoo.com Thu Jan 28 09:22:55 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 28 Jan 2016 15:22:55 +0000 (UTC) Subject: [Histonet] ORO staining in pre- vs. post-fixed tissue In-Reply-To: <4ADAF16E8518764ABF9DCEB73129AA75233DE6EB@WAVE-EMAIL.7thwave.local> References: <4ADAF16E8518764ABF9DCEB73129AA75233DE6EB@WAVE-EMAIL.7thwave.local> Message-ID: <523935558.1336460.1453994575970.JavaMail.yahoo@mail.yahoo.com> Michele:Methodologically it is much more difficult to freeze/section formalin fixed tissue so it is unnecessary adding an additional layer of difficulty to the procedure pre-fixing in formalin.Although I have not tried it, there is no effect of NBF on the amount of fat so there should not be a difference in the reaction of ORO.I think that you have presented this question probably because you have old already fixed tissue samples and now want to use ORO on them and if this is the case, they will be very difficult to make frozen sections on them.The best ORO procedure is to "embed" the unfixed tissue in OCT ? make FS ? fix on NBF vapors ? stain with Harris hematoxylin ? stain with OROPlease not that in my procedure nuclear staining precedes ORO stain to assure better nuclear detail, and there is no interference at all with the fat stain.Fixation should be done with NBF vapors, not liquid. To do that I always placed the water washed and dried FS (that usually are near one end of the slide) inside a Coplin jar with a small amount of NBF at the bottom in a way that the section does not touch the NBF. The capped Coplin jar is placed in an oven at 60?C?during 15 minutes, so the section is fixed by the vapors. Then the section is washed and the staining is performed.Ren??? On Wednesday, January 27, 2016 5:28 PM, Michele Wich via Histonet wrote: Does anyone know whether there is a difference, quantitatively, in Oil red O fat staining of tissue fixed in NBF prior to embedding in OCT vs. fresh frozen tissue embedded in OCT? I know that sections from fresh frozen tissues must be post-fixed in formalin before staining with Oil Red O. But is there any benefit to using this preparation over tissues that are fixed in formalin prior to OCT embedding (aside from sectioning/slide adhesion issues previously described)? Is the staining similar, or does one preparation give better staining results? Much gratitude for any advice you can give, Michele _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JRobinson at pathology-associates.com Thu Jan 28 10:51:40 2016 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Thu, 28 Jan 2016 16:51:40 +0000 Subject: [Histonet] SOX 10 Cellmarque In-Reply-To: <214af96e0c3c48c2973ae78186d2145c@RAMEXCHANGE.HPS.LOCAL> References: <214af96e0c3c48c2973ae78186d2145c@RAMEXCHANGE.HPS.LOCAL> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C90A1209F@PAEXCH1.PathologyAssociates.local> Hi Edison- I have had some headaches getting the SOX-10 to run in the past but I now have all of the kinks ironed out and it works quite well. I had started with the CellMarque mouse monoclonal of their RTU SOX-10. I had it working well and then Leica recalled their red detection kits. I could never get the mouse monoclonal to work again after that with the new red detection kits. BioCare came out with a RTU rabbit monoclonal so I tried that and Bingo!- great staining once again. CellMarque has since come out with their own RTU rabbit monoclonal. They sent me a sample to try and that also stained well but I had already validated the BioCare antibody so I have stayed with that. I run it with ER2 for 30 minutes with the red kit. A word of caution: do not delay when taking those slides off the Bond. Take them off as soon as they are finished, rinse in DI water for a short period of time, run down through the alcohols (I use one 95% and 2 100% ETOH), into the xylene and coverslip immediately. The red stain will "wash" out if you delay running the slides down. This cannot be repaired to the best of my knowledge- you will have to rerun new slides. I use this same protocol for Melan A and it also works great. I use the Leica Melan A (RTU). Good Luck! Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Edison Narvaez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, January 28, 2016 6:13 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] SOX 10 Cellmarque Hello, Anybody running Cellmarque's RTU SOX 10 antibody on the bond III, any protocol suggestions, I would like to run this antibody with Leica's red kit. Thank you ________________________________ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From jaylundgren at gmail.com Thu Jan 28 12:46:06 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 28 Jan 2016 12:46:06 -0600 Subject: [Histonet] unsolved proplem In-Reply-To: References: <1161621322.506108.1453836757971.JavaMail.yahoo.ref@mail.yahoo.com> <1161621322.506108.1453836757971.JavaMail.yahoo@mail.yahoo.com> Message-ID: What kind of processor are you using? On Tue, Jan 26, 2016 at 1:36 PM, Jennifer MacDonald via Histonet < histonet at lists.utsouthwestern.edu> wrote: > There are a couple of things that it might be. > 1. Uneven deparaffinization before staining. > 2. Water in your last reagents/paraffin on the processor. > > > > From: mohamed abd el razik via Histonet > > To: "Histonet at lists.utsouthwestern.edu" > > Date: 01/26/2016 11:33 AM > Subject: [Histonet] unsolved proplem > > > > Dear allI have submitted a proplem about reprocessing tissue blocks that > have patches of stained areas and unstained pale yellow areas (H&E > stain)!!!, unfortently the proplem didn't solved yet and i have tried all > possible solutions, increasing processing time and ordered new chemicals > from other soruces and changed the paraplast company but still have the > proplem !!!! > i'm using 70% alc 2hr - 80% alc 1hr - 90% alc 45mins- absolut 1 and 2 each > for 30 mins then Benzen 1 and 2 each for 25 mins then clearing by methyl > benzoat 2hs at least then paraffin1+2 (Maccormick melting degree > 56-58)each 90 mins for mice kidney, liver and tests > the previous protocol was very satisfactory to us and we have tried to > stain older sections processed befor the emerging proplem and have a nice > stain by current H&E stain to ensure that the proplem is out our staining > step. > any suggestions please !! > Mohamed > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From KSimeone at leavittmgt.com Thu Jan 28 12:56:09 2016 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Thu, 28 Jan 2016 18:56:09 +0000 Subject: [Histonet] SOX 10 Cellmarque (Edison Narvaez) Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CB902C481@vm-email.leavittmgt.com> Hi Edison. I use the CM SOX-10 polyclonal RTU on my Leica Bond-Max(s). I also run it with Leica's red kit. I have had zero issues. I do use CM's SOX-10 controls though, they seem to stain the best for me. My protocol is ER2 for 30 minutes at 97 degrees. I run them down from 100%-SUB-X and have no running or bleeding. Good luck, this protocol has been issue free for me for years. Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor Delray Beach Technical Laboratory ADCS Clinics, LLC Advanced Dermatology and Cosmetic Surgery The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From lmdee1 at yahoo.com Thu Jan 28 13:02:46 2016 From: lmdee1 at yahoo.com (Linda) Date: Thu, 28 Jan 2016 19:02:46 +0000 (UTC) Subject: [Histonet] unsolved proplem In-Reply-To: References: Message-ID: <213152040.1401492.1454007766747.JavaMail.yahoo@mail.yahoo.com> Hello, Did you call the?vendor/manufacturer of your chemicals?? Sometimes, if they have to?dispense the chemicals from a different place it can cause issues.? I had that happen with reagent alcohols. Good luck, Linda Dee, BGS, HT(ASCP) From: Jay Lundgren via Histonet To: Jennifer MacDonald Cc: "Histonet at lists.utsouthwestern.edu" Sent: Thursday, January 28, 2016 12:46 PM Subject: Re: [Histonet] unsolved proplem What kind of processor are you using? On Tue, Jan 26, 2016 at 1:36 PM, Jennifer MacDonald via Histonet < histonet at lists.utsouthwestern.edu> wrote: > There are a couple of things that it might be. > 1.? Uneven deparaffinization before staining. > 2.? Water in your last reagents/paraffin on the processor. > > > > From:? mohamed abd el razik via Histonet > > To:? ? "Histonet at lists.utsouthwestern.edu" > > Date:? 01/26/2016 11:33 AM > Subject:? ? ? ? [Histonet] unsolved proplem > > > > Dear allI have submitted a proplem about reprocessing tissue blocks that > have patches of stained areas and unstained pale yellow areas (H&E > stain)!!!, unfortently the proplem didn't solved yet and i have tried all > possible solutions, increasing processing time and ordered new chemicals > from other soruces and changed the paraplast company but still have the > proplem !!!! > i'm using 70% alc 2hr - 80% alc 1hr - 90% alc 45mins- absolut 1 and 2 each > for 30 mins then Benzen 1 and 2 each for 25 mins then clearing by methyl > benzoat 2hs at least then paraffin1+2 (Maccormick melting degree > 56-58)each 90 mins for mice kidney, liver and tests > the previous protocol was very satisfactory to us and we have tried to > stain older sections processed befor the emerging proplem and have a nice > stain by current H&E stain to ensure that the proplem is out our staining > step. > any suggestions please !! > Mohamed > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ashnews at comcast.net Thu Jan 28 14:50:51 2016 From: ashnews at comcast.net (ashnews at comcast.net) Date: Thu, 28 Jan 2016 20:50:51 +0000 (UTC) Subject: [Histonet] Missouri/Arkansas Joint Meeting May 19th to 21st, 2016 Branson MO In-Reply-To: <778715974.12711397.1454014187720.JavaMail.zimbra@comcast.net> Message-ID: <703656277.12712436.1454014251141.JavaMail.zimbra@comcast.net> Good Afternoon, We would like to invite all of you to Branson Missouri for the first Joint Meeting of Missouri/Arkansas Histology Societies. We will be at the Radisson Hotel right in the middle of everything with the program below. If you have any questions please contact either Sharon Walsh or myself as listed below. Please join us and remember we are also offering a full day for Preparing for the HT Exam with a Tissue ID Class also suggested on Friday afternoon by Shane Jones. May 19, 2026 Thursday 5:30p-7:00p The Joy of Cooking, Ada Feldman, MS, HT/HTL (ASCP) CM, Anatech Ltd, Battle Creek MI Vendor Reception 7:00-9:00 May 20, 2016 Friday AM (8:00-9:30) Session A- Safe Handling of CJD and Prions in the Histology Laboratory, Konnie Zeitner, HT (ASCP) HTL, SLS Session B- Troubleshooting Hematoxylin and Eosin Stain Processing Schedules Revisited- Part 1, Ada Feldman, MS, HT/HTL (ASCP) CM Friday AM (10:30-12:00) Session C- Basic IHC -Title TBA, Misty Lackey BS Biology, Senior Technical Consultant, Cell Marque Corp. Session D- Troubleshooting Hematoxylin and Eosin Stain Processing Schedules Revisited- Part 2, Ada Feldman, MS, HT/HTL (ASCP) CM, Friday PM (1:00-2:30) Session E- Tissue ID- Part 1*, - Shane Jones BS, HT (ASCP), Program Director, Baptist Health School of Histotechnology, AR Preparing for the HT/HTL Exam Part 1 & Part 2 on Saturday. Session F-10 Tips -on how to get the most out of your skin biopsy - Garth Fraga, MD, Associate Professor of Pathology, University of Kansas Health Systems Friday PM (3:30-5:00) Session G- Tissue ID- Part 2, -Shane Jones, BS, HT (ASCP) Session H- Modernizing Anatomic Pathology Dr. Shree Sharma May 21, 2016 Saturday AM (8:00-9:30) Session I- Advanced IHC, Title TBA, Misty Lackey BS Biology, Senior Technical Consultant, Cell Marque Corp., Session J- TBA, Judi Stasko, CLT, Manager, National Animal Disease Center, Region V Director Saturday AM (10:30-12:00) Session K- Ada Feldman, MS, HT/HTL(ASCP)CM Session L- Process Improvement, Christine ?Charlie? Dorner, HT (ASCP), Lean Six Sigma Certified, Agilent Saturday PM (1:00-2:30) Session M- History of Molecular Pathology, Terra Wineman, HTL (ASCP) CM, Research Biologist, Novus International Session N- Use HistoQIP to Improve Quality in your Laboratory, Konnie Zeitner, HT (ASCP) HTL, SLS Saturday PM (2:45-4:15) Session O- Autopsy in America, Dr. Jennifer Forsyth, ME Little Rock, AR May 20, 2016 - Saturday (All Day) 8:00-4:30 Preparing for the HT Exam, Shane Jones, BS, HT From cforster at umn.edu Thu Jan 28 14:58:01 2016 From: cforster at umn.edu (Colleen Forster) Date: Thu, 28 Jan 2016 14:58:01 -0600 Subject: [Histonet] Missouri/Arkansas Joint Meeting May 19th to 21st, 2016 Branson MO In-Reply-To: <703656277.12712436.1454014251141.JavaMail.zimbra@comcast.net> References: <778715974.12711397.1454014187720.JavaMail.zimbra@comcast.net> <703656277.12712436.1454014251141.JavaMail.zimbra@comcast.net> Message-ID: This looks wonderful! Be sure to keep me on your list Pam so I can consider this meeting! C On Thu, Jan 28, 2016 at 2:50 PM, Pamela Marcum via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > > Good Afternoon, > > > > > We would like to invite all of you to Branson Missouri for the first Joint > Meeting of Missouri/Arkansas Histology Societies. We will be at the > Radisson Hotel right in the middle of everything with the program below. If > you have any questions please contact either Sharon Walsh or myself as > listed below. Please join us and remember we are also offering a full day > for Preparing for the HT Exam with a Tissue ID Class also suggested on > Friday afternoon by Shane Jones. > > > > > May 19, 2026 > > Thursday 5:30p-7:00p > > The Joy of Cooking, Ada Feldman, MS, HT/HTL (ASCP) CM, Anatech Ltd, Battle > Creek MI > > Vendor Reception 7:00-9:00 > > > > > May 20, 2016 > > > > > Friday AM (8:00-9:30) > > Session A- Safe Handling of CJD and Prions in the Histology Laboratory, > Konnie Zeitner, HT (ASCP) HTL, SLS > > Session B- Troubleshooting Hematoxylin and Eosin Stain Processing > Schedules Revisited- Part 1, Ada Feldman, MS, HT/HTL (ASCP) CM > > Friday AM (10:30-12:00) > > Session C- Basic IHC -Title TBA, Misty Lackey BS Biology, Senior Technical > Consultant, Cell Marque Corp. > > Session D- Troubleshooting Hematoxylin and Eosin Stain Processing > Schedules Revisited- Part 2, Ada Feldman, MS, HT/HTL (ASCP) CM, > > > > > Friday PM (1:00-2:30) > > Session E- Tissue ID- Part 1*, - Shane Jones BS, HT (ASCP), Program > Director, Baptist Health School of Histotechnology, AR > > Preparing for the HT/HTL Exam Part 1 & Part 2 on Saturday. > > Session F-10 Tips -on how to get the most out of your skin biopsy - Garth > Fraga, MD, Associate Professor of Pathology, > > University of Kansas Health Systems > > Friday PM (3:30-5:00) > > Session G- Tissue ID- Part 2, -Shane Jones, BS, HT (ASCP) > > Session H- Modernizing Anatomic Pathology Dr. Shree Sharma > > > > > May 21, 2016 > > > > > Saturday AM (8:00-9:30) > > Session I- Advanced IHC, Title TBA, Misty Lackey BS Biology, Senior > Technical Consultant, Cell Marque Corp., > > Session J- TBA, Judi Stasko, CLT, Manager, National Animal Disease Center, > Region V Director > > Saturday AM (10:30-12:00) > > Session K- Ada Feldman, MS, HT/HTL(ASCP)CM > > Session L- Process Improvement, Christine ?Charlie? Dorner, HT (ASCP), > Lean Six Sigma Certified, Agilent > > > > > Saturday PM (1:00-2:30) > > Session M- History of Molecular Pathology, Terra Wineman, HTL (ASCP) CM, > Research Biologist, Novus International > > Session N- Use HistoQIP to Improve Quality in your Laboratory, Konnie > Zeitner, HT (ASCP) HTL, SLS > > Saturday PM (2:45-4:15) > > Session O- Autopsy in America, Dr. Jennifer Forsyth, ME Little Rock, AR > > > > > May 20, 2016 - Saturday (All Day) > > 8:00-4:30 Preparing for the HT Exam, Shane Jones, BS, HT > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shinwari_1 at yahoo.com Thu Jan 28 20:42:55 2016 From: shinwari_1 at yahoo.com (Nasir Abbas) Date: Fri, 29 Jan 2016 02:42:55 +0000 (UTC) Subject: [Histonet] Request: Protocol for Cry Sectioning and subsequent Immunoflourescence References: <900764200.2035392.1454035375130.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <900764200.2035392.1454035375130.JavaMail.yahoo@mail.yahoo.com> Dear All,I am dealing with paraffin embedded liver sections (IHC) to identify a desired marker. Unfortunately i am stuck here and donot get any signals despite using positive controls with different concentration of antibodies. So I am turning to Frozen sections which i never try before and i am blank in this field. So could you please help me provide a detailed protocol for? 1) Tissue Preparation and cryosectioning2) Immunoflourescence of cryosections More over, we have all facilities for cryosectioning and immunoflourescence, what i am lacking with is an expert of the field in my lab.? Sincerely?Nasir Abbas ---PhD FellowGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan Avenue?Science Park?Guangzhou, 510530,?Guangdong?P.R.ChinaCell: +86-1312 8296 614 From shinwari_1 at yahoo.com Thu Jan 28 21:04:33 2016 From: shinwari_1 at yahoo.com (Nasir Abbas) Date: Fri, 29 Jan 2016 03:04:33 +0000 (UTC) Subject: [Histonet] Markers for Kupffer cell: Secondary Conjugate Systems References: <1806547832.2095766.1454036673103.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1806547832.2095766.1454036673103.JavaMail.yahoo@mail.yahoo.com> Dear All, I need your guidance in the following issues, Issue No:1I am going to identify two markers on a single cell (macrophage) as well as on two different cells (CD4 and CD8) in a single tissue (costaining). What could be the best secondary conjugate systems for detection in this case (I only have some experience with HRP conjugate). A protocol will be appreciated. Issue No.2what are the specific markers of Kupffer cells? I am working with CD11b and F4/80 for said cells but it seems (found in articles) that these two markers are also expressed by other leukocytes as well, but my job require a very specific marker for kupffer cells so if any one knows please most welcome. Thanks in anticipation. ? Nasir Abbas ---PhD FellowGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan Avenue?Science Park?Guangzhou, 510530,?Guangdong?P.R.ChinaCell: +86-1312 8296 614 From jvickroy at SpringfieldClinic.com Fri Jan 29 09:24:19 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Fri, 29 Jan 2016 15:24:19 +0000 Subject: [Histonet] Nuclear bubbling Message-ID: <9B1A1501A800064397369BD8072E6BCA064F44EB@E2K10DB.springfieldclinic.com> Traditionally we regard nuclear bubbling as incomplete fixation however I'm not so sure that nuclear bubbling can't be caused by additional processing problems. This morning I have some GI biopsies that fixed for nearly 18 hrs that have a large amount of nuclear bubbling. We run the biopsies on a "short run". I am wondering if possibly we are not getting rid of all of the water and therefore our dehydration steps are not long enough? Anybody have an idea besides incomplete fixation? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From melissa at alliedsearchpartners.com Fri Jan 29 10:10:01 2016 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Fri, 29 Jan 2016 16:10:01 +0000 Subject: [Histonet] Histology Supervisor Job in North Carolina Message-ID: Happy Friday Histoland, I am looking for a qualified Histotech to fill a 2nd Shift Histology Supervisor role in North Carolina. Please contact me for more details. Looking for a Tech who is experienced in grossing and has some supervisory/lead/senior experience. Please reach out to me for more details. Thank you and have a great day! Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 From JMaslanka at stpetes.org Fri Jan 29 11:02:32 2016 From: JMaslanka at stpetes.org (Joseph Maslanka) Date: Fri, 29 Jan 2016 10:02:32 -0700 Subject: [Histonet] Training ??? Message-ID: Can anyone share with me any worksheets you use to evaluate new trainees. Embedding, cutting, special staining things like that. How you set target goals? Thanks in advance, Happy Friday. Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. Communication of electronic protected health information (ePHI) is protected under the Health Insurance Portability and Accountability Act (HIPAA) Act of 1996. Electronic mail (e-mail) communication is not encrypted or secure. The HIPAA Security Rule allows for patients to initiate communication of personal health information over this medium and for providers to respond accordingly with the understanding that privacy of communication is not guaranteed. From rjbuesa at yahoo.com Fri Jan 29 11:20:09 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 29 Jan 2016 17:20:09 +0000 (UTC) Subject: [Histonet] Nuclear bubbling In-Reply-To: <9B1A1501A800064397369BD8072E6BCA064F44EB@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA064F44EB@E2K10DB.springfieldclinic.com> Message-ID: <5550896.1871568.1454088010005.JavaMail.yahoo@mail.yahoo.com> "Nuclear bubbling", manifested as round unstained areas in the nucleus, is caused by incomplete dehydration of the section before staining. There is a review on the subject that I cannot find at this moment.Ren? On Friday, January 29, 2016 10:42 AM, "Vickroy, James via Histonet" wrote: Traditionally we regard nuclear bubbling as incomplete fixation however I'm not so sure that nuclear bubbling can't be caused by additional processing problems.? This morning I have some GI biopsies that fixed for nearly 18 hrs that have a large amount of nuclear bubbling.? We run the biopsies on a "short run".? I am wondering if possibly we are not getting rid of all of the water and therefore our dehydration steps are not long enough? Anybody have an idea besides incomplete fixation? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois? 62703 Office:? 217-528-7541, Ext. 15121 Email:? jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at dpspa.com Fri Jan 29 11:42:48 2016 From: criley at dpspa.com (Charles Riley) Date: Fri, 29 Jan 2016 12:42:48 -0500 Subject: [Histonet] Tissue processing question Message-ID: Hello all, I was wondering what everyone uses to secure biopsy and scant tissues through processing. Also what would you recommend placing breast cores in for processing. Having an argument with grossing staff and pathologist about whether to use sponges, tissue paper, or something else. Looking for the best option that will allow for reagents to penetrate tissue and not leave any artifact -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE From wbenton at cua.md Fri Jan 29 12:06:59 2016 From: wbenton at cua.md (Walter Benton) Date: Fri, 29 Jan 2016 18:06:59 +0000 Subject: [Histonet] Tissue processing question In-Reply-To: References: Message-ID: <073ae8e9d1ea4aacbac504f788911a40@MAIL01.GCU-MD.local> We use hair wrapping paper used for perms. It is the same paper called "biopsy wraps," but at a significant price reduction. You can buy a variety of sizes and the wraps do not cause artifacts and are porous enough for ample solution penetration. Biopsy paper comes in blue and other colors, but the hair wraps only come in white. Our overall experience with them has been great. Let me know if you need any other information. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, January 29, 2016 12:43 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Tissue processing question Hello all, I was wondering what everyone uses to secure biopsy and scant tissues through processing. Also what would you recommend placing breast cores in for processing. Having an argument with grossing staff and pathologist about whether to use sponges, tissue paper, or something else. Looking for the best option that will allow for reagents to penetrate tissue and not leave any artifact -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From wbenton at cua.md Fri Jan 29 12:07:05 2016 From: wbenton at cua.md (Walter Benton) Date: Fri, 29 Jan 2016 18:07:05 +0000 Subject: [Histonet] Tissue processing question In-Reply-To: References: Message-ID: <4073ad2b03bc4a579980fc8202c6d964@MAIL01.GCU-MD.local> We use hair wrapping paper used for perms. It is the same paper called "biopsy wraps," but at a significant price reduction. You can buy a variety of sizes and the wraps do not cause artifacts and are porous enough for ample solution penetration. Biopsy paper comes in blue and other colors, but the hair wraps only come in white. Our overall experience with them has been great. Let me know if you need any other information. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, January 29, 2016 12:43 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Tissue processing question Hello all, I was wondering what everyone uses to secure biopsy and scant tissues through processing. Also what would you recommend placing breast cores in for processing. Having an argument with grossing staff and pathologist about whether to use sponges, tissue paper, or something else. Looking for the best option that will allow for reagents to penetrate tissue and not leave any artifact -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From rjbuesa at yahoo.com Fri Jan 29 12:24:50 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 29 Jan 2016 18:24:50 +0000 (UTC) Subject: [Histonet] Tissue processing question In-Reply-To: References: Message-ID: <148032412.1873872.1454091890815.JavaMail.yahoo@mail.yahoo.com> Sponges can cause a compression artifact leaving some sort of "imprint" on the surface of the biopsy, especially kidney and prostate Bx.I my experience tissue paper is the best option. If you are having difficulties with the wrapping, you can use "tea bags".Ren?? On Friday, January 29, 2016 12:54 PM, Charles Riley via Histonet wrote: Hello all, I was wondering what everyone uses to secure biopsy and scant tissues through processing. Also what would you recommend placing breast cores in for processing. Having an argument with grossing staff and pathologist about whether to use sponges, tissue paper, or something else. Looking for the best option that will allow for reagents to penetrate tissue and not leave any artifact -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mills at 3scan.com Fri Jan 29 12:35:52 2016 From: mills at 3scan.com (Caroline Miller) Date: Fri, 29 Jan 2016 10:35:52 -0800 Subject: [Histonet] Tissue processing question In-Reply-To: <073ae8e9d1ea4aacbac504f788911a40@MAIL01.GCU-MD.local> References: <073ae8e9d1ea4aacbac504f788911a40@MAIL01.GCU-MD.local> Message-ID: I really like this type: https://www.fishersci.com/shop/products/starplex-scientific-histoplex-tissue-cassettes-micromesh-chamber-8/p-2782584 (although I buy them from mastertech, but they seem to have dissapeared from their website) They are great for both large tissues, and also biopsies. A long time ago when I worked in a clinical lab we used the tissue paper and I found that if everything was not heated just right the biopsies would stick and things like currettes were hard to scrape up from there, I always thought I was doing the tissue damage yours mills On Fri, Jan 29, 2016 at 10:06 AM, Walter Benton via Histonet < histonet at lists.utsouthwestern.edu> wrote: > We use hair wrapping paper used for perms. It is the same paper called > "biopsy wraps," but at a significant price reduction. You can buy a variety > of sizes and the wraps do not cause artifacts and are porous enough for > ample solution penetration. Biopsy paper comes in blue and other colors, > but the hair wraps only come in white. Our overall experience with them has > been great. > > Let me know if you need any other information. > > > Walter Benton HT(ASCP)QIHC > Lab Operations Manager > Chesapeake Urology Associates > 806 Landmark Drive, Suite 127 > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > Chesapeakeurology.com > > Voted a Best Place to Work by > Baltimore and Modern Healthcare > Magazines. > > > > -----Original Message----- > From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu > ] > Sent: Friday, January 29, 2016 12:43 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Tissue processing question > > Hello all, > > I was wondering what everyone uses to secure biopsy and scant tissues > through processing. Also what would you recommend placing breast cores in > for processing. Having an argument with grossing staff and pathologist > about whether to use sponges, tissue paper, or something else. Looking for > the best option that will allow for reagents to penetrate tissue and not > leave any artifact > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > > Doctors Pathology Services, Dover DE > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > CONFIDENTIALITY NOTICE: The information contained in this electronic > message is intended solely for the personal and confidential use of the > designated recipient(s) named above and may contain information that is > protected from disclosure under applicable law. If you are not the intended > recipient, or the employee or agent responsible for delivering it to the > intended recipient, you are hereby notified that any dissemination, > distribution or copying of this transmission is strictly prohibited. If you > have received this transmission in error, please notify the transmitting > person/department immediately by email or telephone (410) 581-5881 and > delete the message without making a copy. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From wbenton at cua.md Fri Jan 29 12:41:48 2016 From: wbenton at cua.md (Walter Benton) Date: Fri, 29 Jan 2016 18:41:48 +0000 Subject: [Histonet] Tissue processing question In-Reply-To: References: <073ae8e9d1ea4aacbac504f788911a40@MAIL01.GCU-MD.local> Message-ID: <4af9fb71e88e42d4a8ef947665223cce@MAIL01.GCU-MD.local> Histoscreen cassettes will work as well. Generally the cassette options are expensive and may not work in all cassette printers, if you are using one. http://www.thermoscientific.com/content/tfs/en/product/tissue-loc-histoscreen-cassettes.html Ultimately, get samples of whatever you like to use. From: Caroline Miller [mailto:mills at 3scan.com] Sent: Friday, January 29, 2016 1:36 PM To: Walter Benton Cc: Charles Riley ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue processing question I really like this type: https://www.fishersci.com/shop/products/starplex-scientific-histoplex-tissue-cassettes-micromesh-chamber-8/p-2782584 (although I buy them from mastertech, but they seem to have dissapeared from their website) They are great for both large tissues, and also biopsies. A long time ago when I worked in a clinical lab we used the tissue paper and I found that if everything was not heated just right the biopsies would stick and things like currettes were hard to scrape up from there, I always thought I was doing the tissue damage yours mills On Fri, Jan 29, 2016 at 10:06 AM, Walter Benton via Histonet > wrote: We use hair wrapping paper used for perms. It is the same paper called "biopsy wraps," but at a significant price reduction. You can buy a variety of sizes and the wraps do not cause artifacts and are porous enough for ample solution penetration. Biopsy paper comes in blue and other colors, but the hair wraps only come in white. Our overall experience with them has been great. Let me know if you need any other information. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, January 29, 2016 12:43 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Tissue processing question Hello all, I was wondering what everyone uses to secure biopsy and scant tissues through processing. Also what would you recommend placing breast cores in for processing. Having an argument with grossing staff and pathologist about whether to use sponges, tissue paper, or something else. Looking for the best option that will allow for reagents to penetrate tissue and not leave any artifact -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From tejohnson at genoptix.com Fri Jan 29 12:49:07 2016 From: tejohnson at genoptix.com (Teri Johnson) Date: Fri, 29 Jan 2016 18:49:07 +0000 Subject: [Histonet] Nuclear bubbling artifact Message-ID: <1589188c1eb84dc9bd104f66c01aa39f@PHUSCB-SP37MB03.genoptix.org> Hi James, Nuclear bubbling artifact is most commonly seen in formalin fixed epithelial cells, and GI biopsies are among those samples that are particularly susceptible to it. It has been linked to inadequate fixation and also to heating of slides prior to staining without complete air-drying of the tissues. I would recommend cutting the block again, air drying the slides for a time before using heat to melt the wax prior to H&E stain and see if the artifact persists. http://www.cap.org/apps/docs/proficiency_testing/nuclear_bubbling.pdf Best wishes, Teri Johnson ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From mjones at metropath.com Fri Jan 29 12:56:48 2016 From: mjones at metropath.com (Michael Ann Jones) Date: Fri, 29 Jan 2016 18:56:48 +0000 Subject: [Histonet] Tissue processing question In-Reply-To: <4af9fb71e88e42d4a8ef947665223cce@MAIL01.GCU-MD.local> References: <073ae8e9d1ea4aacbac504f788911a40@MAIL01.GCU-MD.local> <4af9fb71e88e42d4a8ef947665223cce@MAIL01.GCU-MD.local> Message-ID: LOL, we used cigarette paper worked fine. Now we use screen cassettes or HistoGel processing if super tiny. Michael Ann Providing collaborative diagnostic services, saving lives today and tomorrow. On 1/29/16, 11:41 AM, "Walter Benton via Histonet" wrote: >Histoscreen cassettes will work as well. Generally the cassette options >are expensive and may not work in all cassette printers, if you are using >one. > >http://www.thermoscientific.com/content/tfs/en/product/tissue-loc-histoscr >een-cassettes.html > >Ultimately, get samples of whatever you like to use. > >From: Caroline Miller [mailto:mills at 3scan.com] >Sent: Friday, January 29, 2016 1:36 PM >To: Walter Benton >Cc: Charles Riley ; histonet at lists.utsouthwestern.edu >Subject: Re: [Histonet] Tissue processing question > >I really like this type: >https://www.fishersci.com/shop/products/starplex-scientific-histoplex-tiss >ue-cassettes-micromesh-chamber-8/p-2782584 >(although I buy them from mastertech, but they seem to have dissapeared >from their website) >They are great for both large tissues, and also biopsies. A long time ago >when I worked in a clinical lab we used the tissue paper and I found that >if everything was not heated just right the biopsies would stick and >things like currettes were hard to scrape up from there, I always thought >I was doing the tissue damage >yours >mills > >On Fri, Jan 29, 2016 at 10:06 AM, Walter Benton via Histonet >u>> wrote: >We use hair wrapping paper used for perms. It is the same paper called >"biopsy wraps," but at a significant price reduction. You can buy a >variety of sizes and the wraps do not cause artifacts and are porous >enough for ample solution penetration. Biopsy paper comes in blue and >other colors, but the hair wraps only come in white. Our overall >experience with them has been great. > >Let me know if you need any other information. > > >Walter Benton HT(ASCP)QIHC >Lab Operations Manager >Chesapeake Urology Associates >806 Landmark Drive, Suite 127 >Glen Burnie, MD 21061 >443-471-5850 (Direct) >410-768-5961 (Lab) >410-768-5965 (Fax) >Chesapeakeurology.com > >Voted a Best Place to Work by >Baltimore and Modern Healthcare >Magazines. > > > >-----Original Message----- >From: Charles Riley via Histonet >[mailto:histonet at lists.utsouthwestern.edutern.edu>] >Sent: Friday, January 29, 2016 12:43 PM >To: >histonet at lists.utsouthwestern.edu> >Subject: [Histonet] Tissue processing question > >Hello all, > > I was wondering what everyone uses to secure biopsy and scant tissues >through processing. Also what would you recommend placing breast cores in >for processing. Having an argument with grossing staff and pathologist >about whether to use sponges, tissue paper, or something else. Looking >for the best option that will allow for reagents to penetrate tissue and >not leave any artifact > >-- > >Charles Riley HT(ASCP)CM > >Histopathology Coordinator/ Mohs > >Doctors Pathology Services, Dover DE >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >CONFIDENTIALITY NOTICE: The information contained in this electronic >message is intended solely for the personal and confidential use of the >designated recipient(s) named above and may contain information that is >protected from disclosure under applicable law. If you are not the >intended recipient, or the employee or agent responsible for delivering >it to the intended recipient, you are hereby notified that any >dissemination, distribution or copying of this transmission is strictly >prohibited. If you have received this transmission in error, please >notify the transmitting person/department immediately by email or >telephone (410) 581-5881 and delete the message >without making a copy. > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >-- >Caroline Miller (mills) >Director of Histology >3Scan.com >415 2187297 >CONFIDENTIALITY NOTICE: The information contained in this electronic >message is intended solely for the personal and confidential use of the >designated recipient(s) named above and may contain information that is >protected from disclosure under applicable law. If you are not the >intended recipient, or the employee or agent responsible for delivering >it to the intended recipient, you are hereby notified that any >dissemination, distribution or copying of this transmission is strictly >prohibited. If you have received this transmission in error, please >notify the transmitting person/department immediately by email or >telephone (410) 581-5881 and delete the message without making a copy. >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA at mercer.edu Fri Jan 29 13:00:05 2016 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Fri, 29 Jan 2016 19:00:05 +0000 Subject: [Histonet] Tissue processing question In-Reply-To: References: <073ae8e9d1ea4aacbac504f788911a40@MAIL01.GCU-MD.local> Message-ID: <912611ce47934923a81bedf7096203b7@spiderman.MercerU.local> Not advertising but I do a lot of research on tiny pieces of tissue and have found the perfect cassette from Cancer Diagnostics. It is the Vortex corner-less ones seen here. http://cancerdiagnostics.com/index.php/cassettes-accessories/microbiopsy-cassettes-and-specialty-cassettes/vortex-biopsy-cassette.html They have two sizes in these. I have to process specimens the size of a gnat's eye and they do not get lost. No corners to deal with. Shirley -----Original Message----- From: Caroline Miller via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, January 29, 2016 1:36 PM To: Walter Benton Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue processing question I really like this type: https://www.fishersci.com/shop/products/starplex-scientific-histoplex-tissue-cassettes-micromesh-chamber-8/p-2782584 (although I buy them from mastertech, but they seem to have dissapeared from their website) They are great for both large tissues, and also biopsies. A long time ago when I worked in a clinical lab we used the tissue paper and I found that if everything was not heated just right the biopsies would stick and things like currettes were hard to scrape up from there, I always thought I was doing the tissue damage yours mills On Fri, Jan 29, 2016 at 10:06 AM, Walter Benton via Histonet < histonet at lists.utsouthwestern.edu> wrote: > We use hair wrapping paper used for perms. It is the same paper called > "biopsy wraps," but at a significant price reduction. You can buy a > variety of sizes and the wraps do not cause artifacts and are porous > enough for ample solution penetration. Biopsy paper comes in blue and > other colors, but the hair wraps only come in white. Our overall > experience with them has been great. > > Let me know if you need any other information. > > > Walter Benton HT(ASCP)QIHC > Lab Operations Manager > Chesapeake Urology Associates > 806 Landmark Drive, Suite 127 > Glen Burnie, MD 21061 > 443-471-5850 (Direct) > 410-768-5961 (Lab) > 410-768-5965 (Fax) > Chesapeakeurology.com > > Voted a Best Place to Work by > Baltimore and Modern Healthcare > Magazines. > > > > -----Original Message----- > From: Charles Riley via Histonet > [mailto:histonet at lists.utsouthwestern.edu > ] > Sent: Friday, January 29, 2016 12:43 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Tissue processing question > > Hello all, > > I was wondering what everyone uses to secure biopsy and scant tissues > through processing. Also what would you recommend placing breast cores > in for processing. Having an argument with grossing staff and > pathologist about whether to use sponges, tissue paper, or something > else. Looking for the best option that will allow for reagents to > penetrate tissue and not leave any artifact > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > > Doctors Pathology Services, Dover DE > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > CONFIDENTIALITY NOTICE: The information contained in this electronic > message is intended solely for the personal and confidential use of > the designated recipient(s) named above and may contain information > that is protected from disclosure under applicable law. If you are not > the intended recipient, or the employee or agent responsible for > delivering it to the intended recipient, you are hereby notified that > any dissemination, distribution or copying of this transmission is > strictly prohibited. If you have received this transmission in error, > please notify the transmitting person/department immediately by email > or telephone (410) 581-5881 and delete the message without making a copy. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff at uropartners.com Fri Jan 29 13:05:01 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 29 Jan 2016 19:05:01 +0000 Subject: [Histonet] Sponges for processing biopsies Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B668F5A@COLOEXCH01.uropartners.local> WE use the biopsy sponges, THOROUGHLY soaked in formalin. They are easy to use and not time consuming either for the grosser or the histologist. About 98% of our volume is prostate biopsies, and we do not see the compression artifact Rene references. Unrelated blog http://tinyurl.com/down0129 A good weekend to all. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From KGoodkowsky at goodwin.edu Fri Jan 29 14:15:46 2016 From: KGoodkowsky at goodwin.edu (Kelli Goodkowsky) Date: Fri, 29 Jan 2016 20:15:46 +0000 Subject: [Histonet] Clearance Angle on Microtome Blade Message-ID: <54068C18-AC6F-42C4-B088-968EE9CC04BF@goodwin.edu> Good afternoon, Can someone touch base with me regarding the clearance angle of the microtome blade? How would you describe a blade clearance angle that is too great or too slight, and what microtomy problems are you likely to see with each? There seems to be conflicting information out there (or at least varying perspectives). Thank you! Kelli Kelli Goodkowsky, M.Ed., HT (ASCP) Director Clinical Education Histologic Science Goodwin College (860) 727-6917 kgoodkowsky at goodwin.edu http://www.goodwin.edu From gu.lang at gmx.at Fri Jan 29 15:23:33 2016 From: gu.lang at gmx.at (Gudrun Lang) Date: Fri, 29 Jan 2016 22:23:33 +0100 Subject: [Histonet] Sponges for processing biopsies In-Reply-To: <6347C6D2B080534F9B5C2B08436DCFAF0B668F5A@COLOEXCH01.uropartners.local> References: <6347C6D2B080534F9B5C2B08436DCFAF0B668F5A@COLOEXCH01.uropartners.local> Message-ID: <001e01d15adb$4f924a20$eeb6de60$@gmx.at> Sponges are available in different qualities. We use very soft ones, that don't hurt the tissue. I know, there are also very hard materials on the market, that may render holes in the underfixed biopsies. Gudrun -----Urspr?ngliche Nachricht----- Von: Lester Raff MD via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Freitag, 29. J?nner 2016 20:05 An: 'histonet at lists.utsouthwestern.edu' Betreff: [Histonet] Sponges for processing biopsies WE use the biopsy sponges, THOROUGHLY soaked in formalin. They are easy to use and not time consuming either for the grosser or the histologist. About 98% of our volume is prostate biopsies, and we do not see the compression artifact Rene references. Unrelated blog http://tinyurl.com/down0129 A good weekend to all. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang at gmx.at Fri Jan 29 15:31:41 2016 From: gu.lang at gmx.at (Gudrun Lang) Date: Fri, 29 Jan 2016 22:31:41 +0100 Subject: [Histonet] Nuclear bubbling artifact In-Reply-To: <1589188c1eb84dc9bd104f66c01aa39f@PHUSCB-SP37MB03.genoptix.org> References: <1589188c1eb84dc9bd104f66c01aa39f@PHUSCB-SP37MB03.genoptix.org> Message-ID: <001f01d15adc$71eb0e30$55c12a90$@gmx.at> I've read about a group, that observed living cells during the fixation-process. They saw bubbling in the first period of contact and penetration of formaldehyde. After a certain time the bubbles disappeared again. Along this observation for me bubbles are a sign of too short fixation. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: Teri Johnson via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Freitag, 29. J?nner 2016 19:49 An: 'histonet at lists.utsouthwestern.edu' Betreff: Re: [Histonet] Nuclear bubbling artifact Hi James, Nuclear bubbling artifact is most commonly seen in formalin fixed epithelial cells, and GI biopsies are among those samples that are particularly susceptible to it. It has been linked to inadequate fixation and also to heating of slides prior to staining without complete air-drying of the tissues. I would recommend cutting the block again, air drying the slides for a time before using heat to melt the wax prior to H&E stain and see if the artifact persists. http://www.cap.org/apps/docs/proficiency_testing/nuclear_bubbling.pdf Best wishes, Teri Johnson ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Sat Jan 30 07:37:15 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Sat, 30 Jan 2016 13:37:15 +0000 (UTC) Subject: [Histonet] Clearance Angle on Microtome Blade In-Reply-To: <54068C18-AC6F-42C4-B088-968EE9CC04BF@goodwin.edu> References: <54068C18-AC6F-42C4-B088-968EE9CC04BF@goodwin.edu> Message-ID: <448245350.2094037.1454161035959.JavaMail.yahoo@mail.yahoo.com> The type of tissue, the speed of sectioning, the knife bevel?and the type of paraffin (melting point) influence the clearance angle.Anywhere from 5 to 10? (preferable 5-6?) are the most used.Ren? On Friday, January 29, 2016 3:17 PM, Kelli Goodkowsky via Histonet wrote: Good afternoon, Can someone touch base with me regarding the clearance angle of the microtome blade?? How would you describe a blade clearance angle that is too great or too slight, and what microtomy problems are you likely to see with each?? There seems to be conflicting information out there (or at least varying perspectives). Thank you! Kelli Kelli Goodkowsky, M.Ed., HT (ASCP) Director Clinical Education Histologic Science Goodwin College (860) 727-6917 kgoodkowsky at goodwin.edu http://www.goodwin.edu/ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barryrittman at gmail.com Sat Jan 30 12:41:41 2016 From: barryrittman at gmail.com (Barry Rittman) Date: Sat, 30 Jan 2016 12:41:41 -0600 Subject: [Histonet] Clearance Angle on Microtome Blade In-Reply-To: <448245350.2094037.1454161035959.JavaMail.yahoo@mail.yahoo.com> References: <54068C18-AC6F-42C4-B088-968EE9CC04BF@goodwin.edu> <448245350.2094037.1454161035959.JavaMail.yahoo@mail.yahoo.com> Message-ID: Rene thank you. There is an excellent book on the section cutting that will give you any theory you need. Section Cutting in Microscopy. by H.F. Steedman. 1960. Blackwell Scientific Publications. Oxford. Also an American Optical Booklet on the Effective Use and Proper Care of the Microtome. Barry On Sat, Jan 30, 2016 at 7:37 AM, Rene J Buesa via Histonet < histonet at lists.utsouthwestern.edu> wrote: > The type of tissue, the speed of sectioning, the knife bevel and the type > of paraffin (melting point) influence the clearance angle.Anywhere from 5 > to 10? (preferable 5-6?) are the most used.Ren? > > On Friday, January 29, 2016 3:17 PM, Kelli Goodkowsky via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Good afternoon, > Can someone touch base with me regarding the clearance angle of the > microtome blade? How would you describe a blade clearance angle that is > too great or too slight, and what microtomy problems are you likely to see > with each? There seems to be conflicting information out there (or at > least varying perspectives). > > Thank you! > Kelli > > Kelli Goodkowsky, M.Ed., HT (ASCP) > Director Clinical Education > Histologic Science > Goodwin College > (860) 727-6917 > kgoodkowsky at goodwin.edu > http://www.goodwin.edu/ > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From KGoodkowsky at goodwin.edu Sat Jan 30 12:52:19 2016 From: KGoodkowsky at goodwin.edu (Kelli Goodkowsky) Date: Sat, 30 Jan 2016 18:52:19 +0000 Subject: [Histonet] Clearance Angle Message-ID: <65D5AC96-5237-4E17-AFB1-F5C6F4581F09@goodwin.edu> I would like to thank you all for your feedback. It was very helpful! Best regards, Kelli Goodkowsky, M.Ed., HT (ASCP) Director Clinical Education Histologic Science Goodwin College (860) 727-6917 kgoodkowsky at goodwin.edu http://www.goodwin.edu From j.rowaihi at alborglaboratories.com Sun Jan 31 01:51:26 2016 From: j.rowaihi at alborglaboratories.com (Jamal Rwaihi) Date: Sun, 31 Jan 2016 10:51:26 +0300 Subject: [Histonet] COMPETENCY ASSESSMENT for HISTOTECHNOLOGIST & CYTOTECHNOLOGIST Message-ID: <002101d15bfc$349d5f30$9dd81d90$@alborglaboratories.com> Dear Colleagues I will be happy if someone share me the current CAP accepted form for: COMPETENCY ASSESSMENT OF HISTOTECHNOLOGIST & CYTOTECHNOLOGIST Best Regards, Jamal M. Al Rowaihi Al Borg Medical Laboratories I Anatomic Pathology Supervisor I Headquarters, Jeddah, KSA I Phone: +966 12 670 0099 I Mobile +966 503629832 www.alborglaboratories.com