From wtaylor8660 at gmail.com Mon Feb 1 06:23:06 2016 From: wtaylor8660 at gmail.com (T Williams) Date: Mon, 1 Feb 2016 07:23:06 -0500 Subject: [Histonet] Storage of Unstained Slides Message-ID: Does anyone have any suggestions as to how unstained slides with patient tissue should be stored? Or could anyone recommend a product for storage? Also - is it preferable that these slides not be placed in the dryer until they are intended to be stained? If so, what is the problem with baking them as soon as they're cut and dried at room temperature? I apologize for all the questions but I do appreciate the guidance. T. Williams From emartinez2 at echd.org Mon Feb 1 09:18:59 2016 From: emartinez2 at echd.org (Estela Martinez) Date: Mon, 1 Feb 2016 15:18:59 +0000 Subject: [Histonet] Destaining IHC's Message-ID: Good Morning ALL, Do anybody have a protocol on destaing IHC's? Is it possible to do that? Need some help! Estela Martinez Histology Supervisor Medical Center Hospital Odessa, TX 79761 432-640-2348 emartinez2 at echd.org CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party without permission of original user and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From Valerie.Hannen at parrishmed.com Mon Feb 1 09:43:29 2016 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Mon, 1 Feb 2016 10:43:29 -0500 Subject: [Histonet] FW: Storage of Unstained Slides In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C323546750B0@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C323546750B0@isexstore03> Message-ID: <450B7A81EDA0C54E97C53D60F00776C323546750B2@isexstore03> -----Original Message----- From: Hannen, Valerie Sent: Monday, February 01, 2016 10:41 AM To: 'T Williams' Subject: RE: [Histonet] Storage of Unstained Slides We store our unstained slides in the drawer with the H&E Slides. We do bake them at @ 64 degrees Celsius for @ 20 minutes before storing them . Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: T Williams via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, February 01, 2016 7:23 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Storage of Unstained Slides Does anyone have any suggestions as to how unstained slides with patient tissue should be stored? Or could anyone recommend a product for storage? Also - is it preferable that these slides not be placed in the dryer until they are intended to be stained? If so, what is the problem with baking them as soon as they're cut and dried at room temperature? I apologize for all the questions but I do appreciate the guidance. T. Williams _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From wdesalvo.cac at outlook.com Mon Feb 1 09:55:47 2016 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Mon, 1 Feb 2016 08:55:47 -0700 Subject: [Histonet] Storage of Unstained Slides Message-ID: I would not heat dry unstained as heat drying removes the paraffin and cells/tissue in no longer protected by the paraffin. Drying slides is a process to remove the sate trapped between the section and glass. Heat drying can speed that process and is also helpful in "baking" tissue to slide to assist in tissue adherence. Air dry until staining is requested, is my advice. Sent from my Windows Phone ________________________________ From: T Williams via Histonet Sent: ?2/?1/?2016 5:31 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Storage of Unstained Slides Does anyone have any suggestions as to how unstained slides with patient tissue should be stored? Or could anyone recommend a product for storage? Also - is it preferable that these slides not be placed in the dryer until they are intended to be stained? If so, what is the problem with baking them as soon as they're cut and dried at room temperature? I apologize for all the questions but I do appreciate the guidance. T. Williams _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ryan.Roy at va.gov Mon Feb 1 10:42:06 2016 From: Ryan.Roy at va.gov (Roy, Ryan) Date: Mon, 1 Feb 2016 11:42:06 -0500 Subject: [Histonet] Salutations from former GC histo sci student Message-ID: <15F883394EAB744E99E1C7E1B9873049018C3256BFE2@R04BYNMSGB1.r04.med.va.gov> Hello Kelli, You may remember me as one of your online graduates from the GC histological science program. How are you? For some reason I thought your involvement with the GC histo program had changed, but seeing your recent post to the histonet it appears your still the director of that program? I hope things are well. You were instrumental in helping me complete that program successfully and were also a good instructor. Ive been at the VA Manchester NH for 2 years now. I am the lone histotech here since the other technician left. Things are going great though we have a critical need for another qualified technician. Please let me know if any of your students especially those already holding a BS would be interested in employment at the VA Hospital in Manchester NH. I could certainly guide them through the application process. Thanks a ton, Ryan Roy HTL (ASCP) Histology Lab Manchester VA 718 Symth Rd Manchester NH 03104 (603) 624-4366 ex 6640 Disclosure: The content of this email does not reflect the policies, views or opinions of the VA. From daniel.floda at mssm.edu Mon Feb 1 15:18:43 2016 From: daniel.floda at mssm.edu (Floda, Daniel) Date: Mon, 1 Feb 2016 21:18:43 +0000 Subject: [Histonet] Technovit 9100 destabilization Message-ID: Hello everyone, With regards to the Technovit 9100 MMA kit, what are the required dimensions and capacity for the chromatography column used in the destabilization step? Also, any general tips or advice regarding the kit would be greatly appreciated. Thank you, Daniel Floda From rennie1108 at yahoo.com Mon Feb 1 15:26:17 2016 From: rennie1108 at yahoo.com (Adrienne Anderson) Date: Mon, 1 Feb 2016 16:26:17 -0500 Subject: [Histonet] GI Pathologist Consulting Message-ID: Hello all, I was wondering if anyone knows of a GI pathologist interested in doing a quick side project as a consultant for the company I work for? If so, please message me privately and I can give you the details. Thanks so much, Adrienne Anderson From sedwards at cellnetix.com Mon Feb 1 15:54:08 2016 From: sedwards at cellnetix.com (Samantha Sackmann) Date: Mon, 1 Feb 2016 21:54:08 +0000 Subject: [Histonet] Glassware Cleaning Message-ID: <19E0EB2535D61B47B1E0C27EBE6927DC30301410@CEL-EXC-004.cellnetix.local> I have tried this procedure multiple times and everytime the solution turns purple in the glassware filled with deionized water, even after rinsing in running water for 2+ minutes. We do not have a dishwasher, so all the glassware is hand-washed. Could there be a problem with our DI water? Has anyone else had an issue with this? Thanks, Samantha Sackmann, CG(ASCP)CMHTLCM, Histotechnologist II CellNetix Pathology & Laboratories 800 West 5th Avenue Spokane, WA 99204 509-473-2311 sedwards at cellnetix.com What is everyone using to test their glassware for detergent residue? Is there a ready to use solution or not? CAP Checklist... Glassware Cleaning Phase II There are appropriate documented procedures for handling and cleaning glassware, including methods for testing for detergent removal. NOTE: Special instructions for micropipettes, cuvets, acid washing, etc. must be included. A simple procedure to check for detergent residue uses bromcresol purple (0.1 g bromcresol purple in 50 mL ethyl alcohol). Pipette approximately 5 cm (2 inches) distilled water into a representative, washed, glassware item. Add two to three drops bromcresol solution. A purple color reveals residual detergent. A yellow color indicates satisfactory rinsing. Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mroark <@t> sfmc.net http://www.sfmc.net Samantha Sackmann, CG(ASCP)CMHTLCM, Histotechnologist II CellNetix Pathology & Laboratories 800 West 5th Avenue Spokane, WA 99204 509-473-2311 sedwards at cellnetix.com DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From patpxs at gmail.com Mon Feb 1 21:46:04 2016 From: patpxs at gmail.com (P Sicurello) Date: Mon, 1 Feb 2016 19:46:04 -0800 Subject: [Histonet] Glassware Cleaning In-Reply-To: <19E0EB2535D61B47B1E0C27EBE6927DC30301410@CEL-EXC-004.cellnetix.local> References: <19E0EB2535D61B47B1E0C27EBE6927DC30301410@CEL-EXC-004.cellnetix.local> Message-ID: We use the bromocresol and it does stay yellow if the pH is correct. We also use a narrow range pH strip. You might want to check the pH of your DI water. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Mon, Feb 1, 2016 at 1:54 PM, Samantha Sackmann via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I have tried this procedure multiple times and everytime the solution > turns purple in the glassware filled with deionized water, even after > rinsing in running water for 2+ minutes. We do not have a dishwasher, so > all the glassware is hand-washed. Could there be a problem with our DI > water? Has anyone else had an issue with this? > > Thanks, > Samantha Sackmann, CG(ASCP)CMHTLCM, Histotechnologist II > CellNetix Pathology & Laboratories > 800 West 5th Avenue > Spokane, WA 99204 > 509-473-2311 > sedwards at cellnetix.com > > > > > > > > > > What is everyone using to test their glassware for detergent residue? Is > there a ready to use solution or not? > > > > > > CAP Checklist... > > > > Glassware Cleaning Phase II > > > > There are appropriate documented procedures for handling and cleaning > glassware, including > > methods for testing for detergent removal. > > > > NOTE: Special instructions for micropipettes, cuvets, acid washing, etc. > must be included. > > > > A simple procedure to check for detergent residue uses bromcresol purple > (0.1 g bromcresol purple > > in 50 mL ethyl alcohol). Pipette approximately 5 cm (2 inches) distilled > water into a representative, > > washed, glassware item. Add two to three drops bromcresol solution. A > purple color reveals residual > > detergent. A yellow color indicates satisfactory rinsing. > > > > Thanks! > > > > > > Matthew Roark- HT/HTL(ASCP)CM > > Histology Specialist > > Saint Francis Medical Center > > 211 Saint Francis Drive > > Cape Girardeau, MO 63703 > > 573-331-3982 > > mroark <@t> sfmc.net< > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > http://www.sfmc.net > > > > > Samantha Sackmann, CG(ASCP)CMHTLCM, Histotechnologist II > CellNetix Pathology & Laboratories > 800 West 5th Avenue > Spokane, WA 99204 > 509-473-2311 > sedwards at cellnetix.com > > DISCLAIMER: > > This message is intended for the sole use of the addressee, and may > contain information that is privileged, confidential and exempt from > disclosure under applicable law. If you are not the addressee you are > hereby notified that you may not use, copy, disclose, or distribute to > anyone the message or any information contained in the message. If you have > received this message in error, please immediately advise the sender by > reply email and delete this message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From laka808 at yahoo.com Tue Feb 2 09:01:28 2016 From: laka808 at yahoo.com (Lisa) Date: Tue, 2 Feb 2016 07:01:28 -0800 Subject: [Histonet] Histonet Digest, Vol 147, Issue 1 In-Reply-To: References: Message-ID: Hi I have a question, I need to find out the pay scale for a certified & non certified histologist in Southern California. Thanks Aloha Lisa Sent from my iPhone > On Feb 1, 2016, at 10:00 AM, histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Storage of Unstained Slides (T Williams) > 2. Destaining IHC's (Estela Martinez) > 3. FW: Storage of Unstained Slides (Hannen, Valerie) > 4. Re: Storage of Unstained Slides (WILLIAM DESALVO) > 5. Salutations from former GC histo sci student (Roy, Ryan) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 1 Feb 2016 07:23:06 -0500 > From: T Williams > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Storage of Unstained Slides > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Does anyone have any suggestions as to how unstained slides with patient > tissue should be stored? Or could anyone recommend a product for > storage? Also - is it preferable that these slides not be placed in the > dryer until they are intended to be stained? If so, what is the problem > with baking them as soon as they're cut and dried at room temperature? > > I apologize for all the questions but I do appreciate the guidance. > > T. Williams > > > ------------------------------ > > Message: 2 > Date: Mon, 1 Feb 2016 15:18:59 +0000 > From: Estela Martinez > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Destaining IHC's > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Good Morning ALL, > > Do anybody have a protocol on destaing IHC's? Is it possible to do that? Need some help! > > > Estela Martinez > Histology Supervisor > Medical Center Hospital > Odessa, TX 79761 > 432-640-2348 > emartinez2 at echd.org > CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party without permission of original user and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. > > > > ------------------------------ > > Message: 3 > Date: Mon, 1 Feb 2016 10:43:29 -0500 > From: "Hannen, Valerie" > To: "Histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] FW: Storage of Unstained Slides > Message-ID: <450B7A81EDA0C54E97C53D60F00776C323546750B2 at isexstore03> > Content-Type: text/plain; charset="us-ascii" > > > > -----Original Message----- > From: Hannen, Valerie > Sent: Monday, February 01, 2016 10:41 AM > To: 'T Williams' > Subject: RE: [Histonet] Storage of Unstained Slides > > We store our unstained slides in the drawer with the H&E Slides. We do bake them at @ 64 degrees Celsius for @ 20 minutes before storing them . > > > > Valerie Hannen,MLT(ASCP),HTL,SU (FL) > Section Chief, Histology > Parrish Medical Center > 951 N. Washington Ave. > Titusville,Florida 32796 > T: (321)268-6333 ext. 7506 > F: (321) 268-6149 > valerie.hannen at parrishmed.com > www.parrishmed.com > > > > -----Original Message----- > From: T Williams via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Monday, February 01, 2016 7:23 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Storage of Unstained Slides > > Does anyone have any suggestions as to how unstained slides with patient tissue should be stored? Or could anyone recommend a product for > storage? Also - is it preferable that these slides not be placed in the > dryer until they are intended to be stained? If so, what is the problem with baking them as soon as they're cut and dried at room temperature? > > I apologize for all the questions but I do appreciate the guidance. > > T. Williams > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ====================================== > "This email is intended solely for the use of the individual to > whom it is addressed and may contain information that is > privileged, confidential or otherwise exempt from disclosure > under applicable law. If the reader of this email is not the > intended recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you > have received this communication in error, please immediately > delete this message. Thank you" > ====================================== > > > > > ------------------------------ > > Message: 4 > Date: Mon, 1 Feb 2016 08:55:47 -0700 > From: WILLIAM DESALVO > To: T Williams , > > Subject: Re: [Histonet] Storage of Unstained Slides > Message-ID: > Content-Type: text/plain; charset="utf-8" > > I would not heat dry unstained as heat drying removes the paraffin and cells/tissue in no longer protected by the paraffin. Drying slides is a process to remove the sate trapped between the section and glass. Heat drying can speed that process and is also helpful in "baking" tissue to slide to assist in tissue adherence. Air dry until staining is requested, is my advice. > > Sent from my Windows Phone > ________________________________ > From: T Williams via Histonet > Sent: ?2/?1/?2016 5:31 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Storage of Unstained Slides > > Does anyone have any suggestions as to how unstained slides with patient > tissue should be stored? Or could anyone recommend a product for > storage? Also - is it preferable that these slides not be placed in the > dryer until they are intended to be stained? If so, what is the problem > with baking them as soon as they're cut and dried at room temperature? > > I apologize for all the questions but I do appreciate the guidance. > > T. Williams > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 5 > Date: Mon, 1 Feb 2016 11:42:06 -0500 > From: "Roy, Ryan" > To: 'Kelli Goodkowsky via Histonet' > > Subject: [Histonet] Salutations from former GC histo sci student > Message-ID: > <15F883394EAB744E99E1C7E1B9873049018C3256BFE2 at R04BYNMSGB1.r04.med.va.gov> > > Content-Type: text/plain; charset="us-ascii" > > Hello Kelli, > > You may remember me as one of your online graduates from the GC histological science program. How are you? For some reason I thought your involvement with the GC histo program had changed, but seeing your recent post to the histonet it appears your still the director of that program? > > I hope things are well. You were instrumental in helping me complete that program successfully and were also a good instructor. Ive been at the VA Manchester NH for 2 years now. I am the lone histotech here since the other technician left. Things are going great though we have a critical need for another qualified technician. Please let me know if any of your students especially those already holding a BS would be interested in employment at the VA Hospital in Manchester NH. I could certainly guide them through the application process. > > Thanks a ton, > > > Ryan Roy HTL (ASCP) > Histology Lab > Manchester VA > 718 Symth Rd > Manchester NH 03104 > (603) 624-4366 ex 6640 > > > Disclosure: The content of this email does not reflect the policies, views or opinions of the VA. > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 147, Issue 1 > **************************************** From cjbulmer2526 at aol.com Tue Feb 2 10:07:16 2016 From: cjbulmer2526 at aol.com (Cindy Bulmer) Date: Tue, 2 Feb 2016 11:07:16 -0500 Subject: [Histonet] Ab vendors Message-ID: <152a2bce8c2-6ede-aa76@webprd-m24.mail.aol.com> Hello Histonet, Has anyone found vendors for Myc and LEF-1 Abs that are not RUOs? Thanks, Cindy Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX From jqb7 at cdc.gov Tue Feb 2 10:16:29 2016 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue, 2 Feb 2016 16:16:29 +0000 Subject: [Histonet] tissue flotation bath Message-ID: <3B2CD438E1628A41BD687E98B963B7814F573090@EMBX-CLFT4.cdc.gov> Hello everyone, I have a Surgipath flotation bath that I love. It is square, black inside, lighted and no glass liner. The things heats up in 5 minutes! I know Surgipath is no longer, but I thought I found a similar product online a year or so ago but now cannot find anything like it. (Except for 1 but has no light) Can anyone direct me? And vendors are more than welcome to reply. Thank you, Jeanine H. Sanders, BS, HT (ASCP), QIHC Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS/G-32 Atlanta, GA 30329 jqb7 at cdc.gov 404-639-3590 From mroark at sfmc.net Tue Feb 2 10:19:49 2016 From: mroark at sfmc.net (Matthew D. Roark) Date: Tue, 2 Feb 2016 16:19:49 +0000 Subject: [Histonet] Missouri Job Opening Message-ID: We are looking for a full time Histo Tech in Cape Girardeau Missouri??. Click to view job posting details. Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-3982 mroark at sfmc.net http://www.sfmc.net From ashnews at comcast.net Tue Feb 2 10:30:27 2016 From: ashnews at comcast.net (=?utf-8?B?YXNobmV3cw==?=) Date: Tue, 2 Feb 2016 16:30:27 GMT Subject: [Histonet] tissue flotation bath Message-ID: <000f4256.42216d4f3105ce5f@comcast.net> Look at Leica. ?We have 6 in our lab.? Sent from my Verizon 4G LTE Smartphone ------ Original message------From: Sanders, Jeanine (CDC/OID/NCEZ...Date: Tue, Feb 2, 2016 10:18 AMTo: histonet at lists.utsouthwestern.edu;Subject:[Histonet] tissue flotation bath Hello everyone,I have a Surgipath flotation bath that I love. It is square, black inside, lighted and no glass liner. The things heats up in 5 minutes!I know Surgipath is no longer, but I thought I found a similar product online a year or so ago but now cannot find anything like it. (Except for 1 but has no light)Can anyone direct me? And vendors are more than welcome to reply.Thank you,Jeanine H. Sanders, BS, HT (ASCP), QIHCCenters for Disease Control and Prevention1600 Clifton Rd., NE MS/G-32Atlanta, GA 30329jqb7 at cdc.gov404-639-3590_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From CBird at amli-denton.com Tue Feb 2 10:33:00 2016 From: CBird at amli-denton.com (Cindy Bird) Date: Tue, 2 Feb 2016 10:33:00 -0600 Subject: [Histonet] (no subject) Message-ID: Hello All, Is there anyone in Histoland that still uses an anti-roll plate for their cryostats. We are issuing "hanging up " Cindy From wbenton at cua.md Tue Feb 2 10:45:27 2016 From: wbenton at cua.md (Walter Benton) Date: Tue, 2 Feb 2016 16:45:27 +0000 Subject: [Histonet] tissue flotation bath In-Reply-To: <3B2CD438E1628A41BD687E98B963B7814F573090@EMBX-CLFT4.cdc.gov> References: <3B2CD438E1628A41BD687E98B963B7814F573090@EMBX-CLFT4.cdc.gov> Message-ID: <489b9c95f18a47f8809f5fe7c14c64cc@MAIL01.GCU-MD.local> http://www.sakura.eu/Our-products/item/8/Microtomy/31/Sakura-Water-Bath-Hot-Plate/Downloads It appears to have a light, but can't tell for sure. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: Sanders, Jeanine (CDC/OID/NCEZID) via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, February 02, 2016 11:16 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] tissue flotation bath Hello everyone, I have a Surgipath flotation bath that I love. It is square, black inside, lighted and no glass liner. The things heats up in 5 minutes! I know Surgipath is no longer, but I thought I found a similar product online a year or so ago but now cannot find anything like it. (Except for 1 but has no light) Can anyone direct me? And vendors are more than welcome to reply. Thank you, Jeanine H. Sanders, BS, HT (ASCP), QIHC Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS/G-32 Atlanta, GA 30329 jqb7 at cdc.gov 404-639-3590 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From plucas at biopath.org Tue Feb 2 11:01:27 2016 From: plucas at biopath.org (Paula) Date: Tue, 2 Feb 2016 09:01:27 -0800 Subject: [Histonet] Transcriptiionist needed Orange County, California Message-ID: <002301d15ddb$5ec5d720$1c518560$@biopath.org> Hello, A very experienced laboratory who has been in the pathology business for over 30 years is looking for a transcriptionist to join our qualified staff. If interested, please contact: Russell Blayney 714-433-1330 phone 714-755-2984 fax rblayney at biopath.org Thank you, Paula Lucas, Lab Manager Biopath Medical Group Fountain Valley, California From Jose.R.deGuzman at gunet.georgetown.edu Tue Feb 2 12:38:23 2016 From: Jose.R.deGuzman at gunet.georgetown.edu (deGuzman, Jose R) Date: Tue, 2 Feb 2016 13:38:23 -0500 Subject: [Histonet] Tissue Flotation bath In-Reply-To: References: Message-ID: Cardinal Health Cat#: M7654-1A MedStar Health is a not-for-profit, integrated healthcare delivery system, the largest in Maryland and the Washington, D.C., region. Nationally recognized for clinical quality in heart, orthopaedics, cancer and GI. IMPORTANT: This e-mail (including any attachments) may contain information that is private, confidential, or protected by attorney-client or other privilege. If you received this e-mail in error, please delete it from your system without copying it and notify sender by reply e-mail, so that our records can be corrected. Thank you. Help conserve valuable resources - only print this email if necessary. From Lesley.Bechtold at jax.org Wed Feb 3 06:38:52 2016 From: Lesley.Bechtold at jax.org (Lesley Bechtold) Date: Wed, 3 Feb 2016 12:38:52 +0000 Subject: [Histonet] BSL2 work? Message-ID: <68767cc708d6457b992027adcc55b3d9@bhexms02.jax.org> Good Morning, Are there any labs out there capable of handling BSL2 level work? In the future, we might have some mouse tissue samples that have been infiltrated with human brain or spinal cord cells. The issue is prions and Creutzfeldt-Jakob disease. We're not set up to handle these samples. Could we outsource them to someone? Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor ME 04609 207-288-6322 The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From Fawn.Bomar at HalifaxRegional.com Wed Feb 3 10:23:25 2016 From: Fawn.Bomar at HalifaxRegional.com (Fawn Bomar) Date: Wed, 3 Feb 2016 16:23:25 +0000 Subject: [Histonet] Temperatures Message-ID: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA1A08B6@EXCH-2K10.hrhs.com> Hi All, I was wondering how everyone tracked their cryostat and paraffin temperatures. Do you all just use the machine temperature, or do you all use the machine and a second thermometer as verification? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From wbenton at cua.md Wed Feb 3 10:57:06 2016 From: wbenton at cua.md (Walter Benton) Date: Wed, 3 Feb 2016 16:57:06 +0000 Subject: [Histonet] Temperatures In-Reply-To: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA1A08B6@EXCH-2K10.hrhs.com> References: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA1A08B6@EXCH-2K10.hrhs.com> Message-ID: Secondary thermometer in paraffin reservoir of embedding center. -----Original Message----- From: Fawn Bomar via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, February 03, 2016 11:23 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Temperatures Hi All, I was wondering how everyone tracked their cryostat and paraffin temperatures. Do you all just use the machine temperature, or do you all use the machine and a second thermometer as verification? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From Toni.Rathborne at rwjuh.edu Wed Feb 3 11:23:30 2016 From: Toni.Rathborne at rwjuh.edu (Rathborne, Toni) Date: Wed, 3 Feb 2016 17:23:30 +0000 Subject: [Histonet] Temperatures In-Reply-To: References: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA1A08B6@EXCH-2K10.hrhs.com> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24332ABD02@vap1014.win.rwjuh.edu> Use a NIST certified thermometer as you would with other lab thermometers for comparison. Toni -----Original Message----- From: Walter Benton via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, February 03, 2016 11:57 AM To: Fawn Bomar; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Temperatures Secondary thermometer in paraffin reservoir of embedding center. -----Original Message----- From: Fawn Bomar via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, February 03, 2016 11:23 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Temperatures Hi All, I was wondering how everyone tracked their cryostat and paraffin temperatures. Do you all just use the machine temperature, or do you all use the machine and a second thermometer as verification? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.Seeley at tenethealth.com Wed Feb 3 12:30:50 2016 From: Heather.Seeley at tenethealth.com (Seeley, Heather) Date: Wed, 3 Feb 2016 18:30:50 +0000 Subject: [Histonet] PPE/ Hair Regulations Message-ID: <9DFE334E776E734D9D231EF60CCE93C57E2578F4@TENHDCTHMB10-31.tenethealth.net> Hello All! Wondering what your lab requires for histology as far as lab coat length and hair policy? We have a bit of a controversy going on and was wanting to get some feedback from fellow histotechs! We are in a hospital and the histology department is located within the lab. Thanks, HEATHER SEELEY, HT(ASCP) Histotechnologist 803-985-4676 OFFICE 803-327-7598 FAX From KBRYAN1 at PARTNERS.ORG Wed Feb 3 13:29:22 2016 From: KBRYAN1 at PARTNERS.ORG (Bryan, Karen) Date: Wed, 3 Feb 2016 19:29:22 +0000 Subject: [Histonet] temperature charts Message-ID: <32AA727CD9EE4F4E82781EB3C238B3085EBEDD60@PHSX10MB16.partners.org> Hi! We have a temperature chart form that we created and use across all instruments and areas. It is one chart for the whole year and has an area for review date and signature at the end of each month. We have daily thermometers. These may be regular thermometers or built in to the instrument. Twice a year we check those daily thermometers against our "gold standard" calibration thermometer. The one we use is a Fisher "Ertco" Standard. Min/Max daily thermometers are also cross checked even if they come with their own calibration report. If the daily is off by +/- 1 deg. C then we replace the daily and monitor it to be sure it is not the instrument that is faulty. We collect all temperature QC Charts and Calibration reports at the end of each year for JCAHO inspection. Hope that helps! Thank you, Karen Karen P. Bryan, HT (ASCP)cm Sr. Neurohistology Specialist Department of Pathology/Amory 3 Brigham & Women's Hospital 75 Francis Street/Boston 617-732-7534 (Internal extension 2-7534) Karen The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From criley at dpspa.com Thu Feb 4 08:50:18 2016 From: criley at dpspa.com (Charles Riley) Date: Thu, 4 Feb 2016 09:50:18 -0500 Subject: [Histonet] PAP stain quality Message-ID: Not sure if anyone out the would know the answer to this. We are having an issue with our PAP stained slides appearing too orange and look aged. If you have any idea for causes I appreciate any help -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE From j.rowaihi at alborglaboratories.com Thu Feb 4 09:22:05 2016 From: j.rowaihi at alborglaboratories.com (Jamal Rowaihi) Date: Thu, 04 Feb 2016 18:22:05 +0300 Subject: [Histonet] PAP stain quality Message-ID: You need to review the slides fixation and what chemicals you are using Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories?Sent from my cell phone-------- Original message --------From: Charles Riley via Histonet Date: 2/4/2016 5:50 PM (GMT+03:00) To: histonet at lists.utsouthwestern.edu Subject: [Histonet] PAP stain quality Not sure if anyone out the would know the answer to this. We are having an issue with our PAP stained slides appearing too orange and look aged. If you have any idea for causes I appreciate any help -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 at cdc.gov Thu Feb 4 09:54:36 2016 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Thu, 4 Feb 2016 15:54:36 +0000 Subject: [Histonet] Slide printer question Message-ID: <3B2CD438E1628A41BD687E98B963B7814F57410C@EMBX-CLFT4.cdc.gov> All, What is the fastest slide printer out there that can print large numbers of slides on any one block and allow you to continuously add the new cases back to back without delay. Thanks, Jeanine H. Sanders, BS, HT (ASCP), QIHC Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS/G-32 Atlanta, GA 30329 jqb7 at cdc.gov 404-639-3590 From histology81176 at att.net Thu Feb 4 12:09:03 2016 From: histology81176 at att.net (Histology Technician) Date: Thu, 4 Feb 2016 18:09:03 +0000 (UTC) Subject: [Histonet] Medica DI water system with Symphony References: <2081901822.1486449.1454609343684.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <2081901822.1486449.1454609343684.JavaMail.yahoo@mail.yahoo.com> Just curious to see if any of you have a Medica DI water system with your symphony?? I was wondering what QA/QC I need to do to it and an SOP.? I'm the only lab in my building, so if I have to test the water, I'd have to take it to a nearby hospital.? Thanks!? This is the first DI water system I've had in the lab and I'm not sure where to start with the maintenance.? From elaineahoffman55 at yahoo.com Thu Feb 4 12:22:38 2016 From: elaineahoffman55 at yahoo.com (Elaine allison Hoffman) Date: Thu, 4 Feb 2016 18:22:38 +0000 (UTC) Subject: [Histonet] Pay Scale References: <1113895804.1454410.1454610158817.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1113895804.1454410.1454610158817.JavaMail.yahoo@mail.yahoo.com> Greetings all, I need to get an update on the most recent pay scale for a certified histotech vs a non-certified histotech in Northern Ohio region.? Also other various descriptions as well, like starting pay, experienced histotech pay, manager/supervisor pay, HT vs HTL, etc.Any input would be appreciated. Thanks! Elaine Hoffman From rsrichmond at gmail.com Thu Feb 4 12:25:39 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 4 Feb 2016 13:25:39 -0500 Subject: [Histonet] Pap stain quality Message-ID: Charles Riley HT(ASCP)CM, Histopathology Coordinator/ Mohs, Doctors Pathology Services, Dover DE asks: >>Not sure if anyone out there would know the answer to this. We are having an issue with our PAP stained slides appearing too orange and looking aged. If you have any idea for causes I appreciate any help.<< The Pap stain (short for Papanicolaou, not an acronym, not capitalized) requires frequent adjustments - it's somewhat finicky at best. A cytotechnologist should know how to trouble-shoot the Pap stain - if you're using the stain, I suppose you have a cytotechnologist available. Bob Richmond Samurai Pathologist Maryville TN From Lesley.Bechtold at jax.org Thu Feb 4 12:31:05 2016 From: Lesley.Bechtold at jax.org (Lesley Bechtold) Date: Thu, 4 Feb 2016 18:31:05 +0000 Subject: [Histonet] Organizing IHC Message-ID: Good Afternoon, I have a question and I'm hoping for some creative answers. How do other labs track their IHC? Right now, we have 3 large binders with the paper work-up sheets and package information filed alphabetically. We have nothing electronic other than an Excel spreadsheet with optimized antibodies listed on it. I'm wondering if there is something out there that other people use rather than paper sheets correlated to an Excel file. Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor ME 04609 207-288-6322 The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From k84as at yahoo.com Thu Feb 4 12:52:21 2016 From: k84as at yahoo.com (mohamed abd el razik) Date: Thu, 4 Feb 2016 18:52:21 +0000 (UTC) Subject: [Histonet] clearing time References: <244553170.1788402.1454611941756.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <244553170.1788402.1454611941756.JavaMail.yahoo@mail.yahoo.com> Dear all what is your optimal clearing time in xelene for prostate and tests of dog samples (about 0.5 cm) ??is there a need for additional clearing in methyl benzoat ? thanks Mohamed From liz at premierlab.com Thu Feb 4 12:56:31 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Thu, 4 Feb 2016 11:56:31 -0700 Subject: [Histonet] Organizing IHC In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02854EF541BB@SBS2K8.premierlab.local> Lesley We have a folder for each antibody, this folder contains all of the information on that antibody. It includes all of our protocol development information, the slides from protocol development in the flat plastic holders, spec sheets, internet searches, publications and references. All of the documentation and a copy of the current SOP. If you do not want to have a physical folder this can all be done digitally for the paperwork and then if you have a scanner the slides from protocol development and validation are scanned, and then everything is digital. We scan all of our protocol development and validation slides for IHC since sometimes we may have to return those to the client. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Lesley Bechtold via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 04, 2016 11:31 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Organizing IHC Good Afternoon, I have a question and I'm hoping for some creative answers. How do other labs track their IHC? Right now, we have 3 large binders with the paper work-up sheets and package information filed alphabetically. We have nothing electronic other than an Excel spreadsheet with optimized antibodies listed on it. I'm wondering if there is something out there that other people use rather than paper sheets correlated to an Excel file. Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor ME 04609 207-288-6322 The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz at premierlab.com Thu Feb 4 13:21:31 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Thu, 4 Feb 2016 12:21:31 -0700 Subject: [Histonet] clearing time In-Reply-To: <244553170.1788402.1454611941756.JavaMail.yahoo@mail.yahoo.com> References: <244553170.1788402.1454611941756.JavaMail.yahoo.ref@mail.yahoo.com> <244553170.1788402.1454611941756.JavaMail.yahoo@mail.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE02854EF541BE@SBS2K8.premierlab.local> Mohamed For larger samples we have ranges from 1.5 hours to 6 hours a station. Anytime we have larger samples we always utilize three absolute alcohols and three xylenes, for your samples I would try 1.5 to 2 hours per station to start. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: mohamed abd el razik via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 04, 2016 11:52 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] clearing time Dear all what is your optimal clearing time in xelene for prostate and tests of dog samples (about 0.5 cm) ??is there a need for additional clearing in methyl benzoat ? thanks Mohamed _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks at gmail.com Thu Feb 4 16:07:06 2016 From: amosbrooks at gmail.com (Amos Brooks) Date: Thu, 4 Feb 2016 17:07:06 -0500 Subject: [Histonet] PPE/ Hair Regulations Message-ID: If it is actually getting caught in things or falling in waterbaths or embedding centers and such, then it needs to be tied back. The same is true of food service. You can't risk contamination. If it is under control though, leave it alone. Don't try to use safety to enforce your personal bias of hair style. https://www.youtube.com/watch?v=aVh_XhwVPNg It's just a youtube song, not a virus you can click it "Ain't cuttin' my hair 'till the good lord comes" Amos On Thu, Feb 4, 2016 at 1:00 PM, wrote: > > Message: 1 > Date: Wed, 3 Feb 2016 18:30:50 +0000 > From: "Seeley, Heather" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] PPE/ Hair Regulations > Message-ID: > < > 9DFE334E776E734D9D231EF60CCE93C57E2578F4 at TENHDCTHMB10-31.tenethealth.net> > > Content-Type: text/plain; charset="iso-8859-1" > > Hello All! > > > > Wondering what your lab requires for histology as far as lab coat length > and hair policy? We have a bit of a controversy going on and was wanting to > get some feedback from fellow histotechs! We are in a hospital and the > histology department is located within the lab. > > > > Thanks, > > > HEATHER SEELEY, HT(ASCP) > Histotechnologist From bethcoxx at gmail.com Thu Feb 4 17:06:53 2016 From: bethcoxx at gmail.com (Beth Cox) Date: Thu, 4 Feb 2016 18:06:53 -0500 Subject: [Histonet] PAP staining quality Message-ID: <56B3D98D.1000607@gmail.com> Hi Charles, A couple things to check on: 1. The first concern I would have is your EA stain. Poor EA staining will give too much orange staining and pale other counterstains (making them look aged). What brand are you using? Have you changed brands? Is your EA close to the expiration date? Is the bulk stored with light exposure? I think fixing your EA will fix all the other problems. 2. The other question I have regards your alcohol. Have you changed types/brands? Pap staining is very delicate and the different alcohols used can make a big difference. Beth Cox, HTL/SCT(ASCP)QIHC ------------------------------ Message: 3 Date: Thu, 4 Feb 2016 09:50:18 -0500 From: Charles Riley To: histonet at lists.utsouthwestern.edu Subject: [Histonet] PAP stain quality Message-ID: Content-Type: text/plain; charset=UTF-8 Not sure if anyone out the would know the answer to this. We are having an issue with our PAP stained slides appearing too orange and look aged. If you have any idea for causes I appreciate any help -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE From hans at histologistics.com Fri Feb 5 12:25:47 2016 From: hans at histologistics.com (Hans B Snyder) Date: Fri, 5 Feb 2016 13:25:47 -0500 Subject: [Histonet] Hypercenter XP pdf manual Message-ID: Hello All, I have been contacted by a colleague in need of a shandon hypercenter XP pdf manual. Does anyone have one they could email to me? I would be very appreciative. Best wishes Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans at histologistics.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From tbraud at holyredeemer.com Fri Feb 5 12:56:09 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 5 Feb 2016 18:56:09 +0000 Subject: [Histonet] PAP stain troubleshooting Message-ID: <48E053DDF6CE074DB6A7414BA05403F805E548@HRHEX02-HOS.holyredeemer.local> Hi Charles - Not to worry. Many of us Histo folks don't have a Cytologist to help. Beth is spot on in her advice. I just wanted to add Orange G should appear yellow to orange; 15 sec to 1 minute is the usual range of staining times. EA is often problematic because of fundamental limitations in its chemical composition. Ideally, one should see clearcut hues of green and red in separate cells. Staining times less than about 3 minutes usually favor the uptake of eosin, with eosin and light green often occupying different areas of the same cells. Most EA formulations perform optimally in the 6-8 minute range. Note particularly that the OG and EA staining times are interdependent: relatively too much time in OG will overload cells with orange G and block the subsequent uptake of eosin. Make sure you record your lots of stain as they are changed out, and try using a self-made buccal smear to check new lots of of the stain components before they are put into use. Then, if you see a test problem, you can repeat the buccal smear and compare to the original. It may help you to pinpoint the problem. Best of luck - Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 9. PAP staining quality (Beth Cox) Message: 9 Date: Thu, 4 Feb 2016 18:06:53 -0500 From: Beth Cox Subject: [Histonet] PAP staining quality Hi Charles, A couple things to check on: 1. The first concern I would have is your EA stain. Poor EA staining will give too much orange staining and pale other counterstains (making them look aged). What brand are you using? Have you changed brands? Is your EA close to the expiration date? Is the bulk stored with light exposure? I think fixing your EA will fix all the other problems. 2. The other question I have regards your alcohol. Have you changed types/brands? Pap staining is very delicate and the different alcohols used can make a big difference. Beth Cox, HTL/SCT(ASCP)QIHC ------------------------------ Message: 3 Date: Thu, 4 Feb 2016 09:50:18 -0500 From: Charles Riley Subject: [Histonet] PAP stain quality Not sure if anyone out the would know the answer to this. We are having an issue with our PAP stained slides appearing too orange and look aged. If you have any idea for causes I appreciate any help -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE ------------------------------ From djemge11 at gmail.com Fri Feb 5 13:36:16 2016 From: djemge11 at gmail.com (Donna Emge) Date: Fri, 5 Feb 2016 14:36:16 -0500 Subject: [Histonet] New Tissue Processor Validation Protocol / SOP Message-ID: Would someone be willing to share their approved SOP method for validation of a new tissue processor before using it with patient tissue? Also, the approved SOP for validation of changes to a tissue processing program. I need this for CAP. How do I find recommendations for this on the CAP website for future reference? Thank you, Donna -- *Donna J. Emge, HT(ASCP)* Histology Manager South Bend Medical Foundation (574) 234-4176 ext. 1345 DEmge at sbmf.org From rsrichmond at gmail.com Sat Feb 6 13:32:16 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 6 Feb 2016 14:32:16 -0500 Subject: [Histonet] Pap stain troubleshooting Message-ID: Thanks for these excellent comments on troubleshooting the EA and OG-6 components I've copied them for my files. The important thing is to have frequent feedback from the cytotechnologist to whoever is doing the staining. In one job I had the staining was done by a clerical person who took great pride in the fact that she'd never looked down a microscope in her life. (She never took a day's vacation - always an eosinophlic clupeid, as any accountant will tell you - got caught with her hand in the till - I hope her jump suit's dyed with orange G.) The cytotechnologists were moonlighters in a distant place - we saw them once a year at the Christmas party. I got burned doing the 10% QC - we had no way to feed back if we found an error - we all missed a cervical adenocarcinoma - only time I've been sued in >50 years in practice. In another crazy practice the cytotechnologist only worked at night, and was forbidden - for reasons I never understood - to ever be in the hospital in the daytime - she'd never even met the histotechnologist who did her staining, and who never looked at a slide. Fortunately we didn't get sued, but we certainly could have. Bob Richmond Samurai Pathologist Maryville TN From kristyn.ferber at gmail.com Mon Feb 8 07:15:21 2016 From: kristyn.ferber at gmail.com (Kristyn Ferber) Date: Mon, 8 Feb 2016 08:15:21 -0500 Subject: [Histonet] C3 IF staining of FFPE tissue Message-ID: Hello List, I suspect it may be a lost cause (since processing likely kills the antigen site) but has anyone had any luck with IF staining for C3 on FFPE tissue? I know frozen is ideal but occasionally, we need to revert to the FFPE sample. Best, Kristyn From dayuang at rci.rutgers.edu Mon Feb 8 10:52:28 2016 From: dayuang at rci.rutgers.edu (dayuang) Date: Mon, 8 Feb 2016 11:52:28 -0500 Subject: [Histonet] Issue with Leica/Jung Histoembedder Message-ID: <93D4868D-8741-4586-9F9A-0CC155D3AC3C@rci.rutgers.edu> Dear all, We have a Leica/Jung Histoembedder, and it stopped working twice recently during embedding. The symptoms are: illumination light went off, hot and cold plates stopped working, the wax dispenser didn?t flow anymore, wax reservoir stopped heating. There was no error message displayed. Switching the power off and on corrected the problem immediately at the first time. However for the 2nd onset, we had to wait for hours until it came back. Please kindly share your experience if anybody has undergone and resolved the problem. I am more than grateful to your kind input. Best regards, Dayuan ******************************************* Dayuan Gao, Ph.D. Assistant Research Professor Department of Pharmaceutics Ernest Mario School of Pharmacy Rutgers, The State University of New Jersey 160 Frelinghuysen Road Piscataway, New Jersey 08854 Phone: (848)445-6837 Fax: (732)445-3134 ******************************************* From relia1 at earthlink.net Mon Feb 8 11:44:26 2016 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 8 Feb 2016 12:44:26 -0500 Subject: [Histonet] RELIA Histology Careers Bulletin Special Edition for Managers and Supervisors - 2-8-2016 Message-ID: <05ce01d16298$5ac424d0$104c6e70$@earthlink.net> Hi Histopeeps! What would make the perfect management position? ? Is it the location? ? Perhaps the type of lab? ? How about the size of the staff/lab? ? Maybe it?s the Hours? The Money and/or benefits? ? Could it be that a particular situation is the next logical step in your career? More than likely the answer is all of the above in different degrees. That is why I am contacting you with this special bulletin. I am starting to get a lot of management opportunities and I wanted to touch base and let you know. Your next opportunity might be just around the corner and I might have it for you. If you are looking for a position right now please contact me right away. We can talk about my current positions OR about a customized search on your behalf. If you aren?t looking right away but want to let me know what would make a perfect job for you so that I could keep an eye out, that would be great too. To do that just shoot me an email at relia1 at earthlink.net or call me toll free at 866-607-3542. Here is a list of my current managerial opportunities: Lab Manager ? Williamsburg, VA Pathology Supervisor ? Manchester, NH Histology Supervisor ? Amarillo, TX Lead Tech/Supervisor ? Fayetteville, AR Sr. Clinical Lab Specialist/Pathology ? Norfolk, VA IHC Tech Support ? Los Angeles, CA All of these clients offer autonomy in your position and a staff eager to welcome their new manager. I really appreciate you taking time out of your busy day to read my e-mail Thanks Again!? Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From jamie.erickson at abbvie.com Mon Feb 8 12:57:23 2016 From: jamie.erickson at abbvie.com (Erickson, Jamie E) Date: Mon, 8 Feb 2016 18:57:23 +0000 Subject: [Histonet] C3 IF staining of FFPE tissue (Kristyn Ferber) Message-ID: <349cd9ddf3c1432ba31db142e110535b@USAASECSM025.R0018.COLLABORATION.ECS.HP.COM> Hello, Kristyn, I don't know what species you work with but I work in mouse (mouse lupus kidneys) and had good luck with Dakos C3c antibody F0201 using Dakos Proteinase K 10 minutes at room temperature. I used a unlabeled version that is not available anymore but F0201 (FITC) one is said to be of the same clone. I had aliquot lots of the old antibody that just ran out so I have not tried this FITC one yet. If you want to stain using DAB I've used a Rabbit anti-FITC (Invitrogen) and then come back with Anti-rabbit HRP polymer then DAB. Its works nice when you have a FITC primary but don't want to visualize with Fluorescence due to background etc.. We have tested a lot of Ab's on paraffin for various epitopes and have a good success rate you just may have to test some antibodies and a few pretreatment conditions (EDTA, Citrate, PTK, Protease 1) we use a Leica Bond autostainer. Best of luck. Jamie Erickson Scientist Abbvie bioresearch Center Worcester, Ma 01605 -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Monday, February 08, 2016 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 147, Issue 8 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. C3 IF staining of FFPE tissue (Kristyn Ferber) 2. Issue with Leica/Jung Histoembedder (dayuang) 3. RELIA Histology Careers Bulletin Special Edition for Managers and Supervisors - 2-8-2016 (Pam Barker) ---------------------------------------------------------------------- Message: 1 Date: Mon, 8 Feb 2016 08:15:21 -0500 From: Kristyn Ferber To: histonet at lists.utsouthwestern.edu Subject: [Histonet] C3 IF staining of FFPE tissue Message-ID: Content-Type: text/plain; charset=UTF-8 Hello List, I suspect it may be a lost cause (since processing likely kills the antigen site) but has anyone had any luck with IF staining for C3 on FFPE tissue? I know frozen is ideal but occasionally, we need to revert to the FFPE sample. Best, Kristyn ------------------------------ Message: 2 Date: Mon, 8 Feb 2016 11:52:28 -0500 From: dayuang To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Issue with Leica/Jung Histoembedder Message-ID: <93D4868D-8741-4586-9F9A-0CC155D3AC3C at rci.rutgers.edu> Content-Type: text/plain; charset=utf-8 Dear all, We have a Leica/Jung Histoembedder, and it stopped working twice recently during embedding. The symptoms are: illumination light went off, hot and cold plates stopped working, the wax dispenser didn?t flow anymore, wax reservoir stopped heating. There was no error message displayed. Switching the power off and on corrected the problem immediately at the first time. However for the 2nd onset, we had to wait for hours until it came back. Please kindly share your experience if anybody has undergone and resolved the problem. I am more than grateful to your kind input. Best regards, Dayuan ******************************************* Dayuan Gao, Ph.D. Assistant Research Professor Department of Pharmaceutics Ernest Mario School of Pharmacy Rutgers, The State University of New Jersey 160 Frelinghuysen Road Piscataway, New Jersey 08854 Phone: (848)445-6837 Fax: (732)445-3134 ******************************************* ------------------------------ Message: 3 Date: Mon, 8 Feb 2016 12:44:26 -0500 From: "Pam Barker" To: Subject: [Histonet] RELIA Histology Careers Bulletin Special Edition for Managers and Supervisors - 2-8-2016 Message-ID: <05ce01d16298$5ac424d0$104c6e70$@earthlink.net> Content-Type: text/plain; charset="iso-8859-1" Hi Histopeeps! What would make the perfect management position? ? Is it the location? ? Perhaps the type of lab? ? How about the size of the staff/lab? ? Maybe it?s the Hours? The Money and/or benefits? ? Could it be that a particular situation is the next logical step in your career? More than likely the answer is all of the above in different degrees. That is why I am contacting you with this special bulletin. I am starting to get a lot of management opportunities and I wanted to touch base and let you know. Your next opportunity might be just around the corner and I might have it for you. If you are looking for a position right now please contact me right away. We can talk about my current positions OR about a customized search on your behalf. If you aren?t looking right away but want to let me know what would make a perfect job for you so that I could keep an eye out, that would be great too. To do that just shoot me an email at relia1 at earthlink.net or call me toll free at 866-607-3542. Here is a list of my current managerial opportunities: Lab Manager ? Williamsburg, VA Pathology Supervisor ? Manchester, NH Histology Supervisor ? Amarillo, TX Lead Tech/Supervisor ? Fayetteville, AR Sr. Clinical Lab Specialist/Pathology ? Norfolk, VA IHC Tech Support ? Los Angeles, CA All of these clients offer autonomy in your position and a staff eager to welcome their new manager. I really appreciate you taking time out of your busy day to read my e-mail Thanks Again!? Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 147, Issue 8 **************************************** From elaineahoffman55 at yahoo.com Mon Feb 8 13:45:43 2016 From: elaineahoffman55 at yahoo.com (Elaine allison Hoffman) Date: Mon, 8 Feb 2016 19:45:43 +0000 (UTC) Subject: [Histonet] Fw: Validation In-Reply-To: <1703119699.849675.1454960250557.JavaMail.yahoo@mail.yahoo.com> References: <1703119699.849675.1454960250557.JavaMail.yahoo.ref@mail.yahoo.com> <1703119699.849675.1454960250557.JavaMail.yahoo@mail.yahoo.com> Message-ID: <51950655.846479.1454960743443.JavaMail.yahoo@mail.yahoo.com> On Monday, February 8, 2016 2:37 PM, Elaine allison Hoffman wrote: Hello everyone, Would anyone be willing to share their validation protocols with me?? We are a small GI lab and we will be moving our lab to another location, into a new building.? So I believe that I must do previous validations before the move and then another after the move, and then there should be a comparison, but I'm not completely sure.? Does anybody know the process of validation before, and after a move? Or does anybody know the correct procedure to comply with regulations on this kind of situation?Any suggestions are greatly appreciated. Thanks, Elaine Hoffman From jillanne80 at msn.com Mon Feb 8 14:00:27 2016 From: jillanne80 at msn.com (Jillian Russell) Date: Mon, 8 Feb 2016 20:00:27 +0000 Subject: [Histonet] California Job Openings Message-ID: Hello All, I currently have two job postings open, one for a Histologist and the other for a Laboratory Assistant for the research laboratory at Dako North America. Please click on the links below if interested for job details and to apply. Histologist- https://goo.gl/todRZb Lab Assistant- https://goo.gl/fIHSu4 Thank you, Jillian A. Russell Jillian A. Russell, HT (ASCP)CM, QIHC Histology Supervisor, Companion Diagnostics, R&D Diagnostics & Genomics Agilent Technologies Dako North America, Inc., an Agilent Technologies Company 6392 Via Real | Carpinteria | CA 93013 | USA +1 805 566-6655 Reception www.dako.com www.agilent.com From jillanne80 at msn.com Mon Feb 8 14:46:47 2016 From: jillanne80 at msn.com (Jillian Russell) Date: Mon, 8 Feb 2016 20:46:47 +0000 Subject: [Histonet] California Job Openings- UPDATE Message-ID: Hello, I don't know what happened to the Laboratory Assistant link? This is the correct one. Lab Assistant- https://goo.gl/fIHSu4 Thank you, Jillian A. Russell Jillian A. Russell, HT (ASCP)CM, QIHC Histology Supervisor, Companion Diagnostics, R&D Diagnostics & Genomics Agilent Technologies Dako North America, Inc., an Agilent Technologies Company 6392 Via Real | Carpinteria | CA 93013 | USA +1 805 566-6655 Reception www.dako.com www.agilent.com From dayuang at rci.rutgers.edu Tue Feb 9 14:32:02 2016 From: dayuang at rci.rutgers.edu (dayuang) Date: Tue, 9 Feb 2016 15:32:02 -0500 Subject: [Histonet] Solved issue with Leica/Jung Histoembedder, topic #2=Re: Histonet Digest, Vol 147, Issue 8 In-Reply-To: References: Message-ID: <7AD00B1D-BCFD-47C3-97B0-769D292D3401@rci.rutgers.edu> Dear all, Thank you very much for the helpful comments. I finally solved the problem by reset the start/end work timer. I would like to thank in particular Paula K. Pierce at Excalibur Pathology Inc, and Jc Dyson at JMD Histology for their kindness and expertise. Best regards, Dayuan ******************************************* Dayuan Gao, Ph.D. Assistant Research Professor Department of Pharmaceutics Ernest Mario School of Pharmacy Rutgers, The State University of New Jersey 160 Frelinghuysen Road Piscataway, New Jersey 08854 Phone: (848)445-6837 Fax: (732)445-3134 ******************************************* > On Feb 8, 2016, at 1:00 PM, histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. C3 IF staining of FFPE tissue (Kristyn Ferber) > 2. Issue with Leica/Jung Histoembedder (dayuang) > 3. RELIA Histology Careers Bulletin Special Edition for Managers > and Supervisors - 2-8-2016 (Pam Barker) > > From: Kristyn Ferber > Subject: [Histonet] C3 IF staining of FFPE tissue > Date: February 8, 2016 at 8:15:21 AM EST > To: histonet at lists.utsouthwestern.edu > > > Hello List, > > I suspect it may be a lost cause (since processing likely kills the antigen > site) but has anyone had any luck with IF staining for C3 on FFPE tissue? I > know frozen is ideal but occasionally, we need to revert to the FFPE > sample. > > Best, > > Kristyn > > > > > From: dayuang > Subject: [Histonet] Issue with Leica/Jung Histoembedder > Date: February 8, 2016 at 11:52:28 AM EST > To: histonet at lists.utsouthwestern.edu > > > Dear all, > > We have a Leica/Jung Histoembedder, and it stopped working twice recently during embedding. The symptoms are: illumination light went off, hot and cold plates stopped working, the wax dispenser didn?t flow anymore, wax reservoir stopped heating. There was no error message displayed. Switching the power off and on corrected the problem immediately at the first time. However for the 2nd onset, we had to wait for hours until it came back. > > Please kindly share your experience if anybody has undergone and resolved the problem. I am more than grateful to your kind input. > > Best regards, > Dayuan > > > > ******************************************* > Dayuan Gao, Ph.D. > Assistant Research Professor > Department of Pharmaceutics > Ernest Mario School of Pharmacy > Rutgers, The State University of New Jersey > 160 Frelinghuysen Road > Piscataway, New Jersey 08854 > Phone: (848)445-6837 > Fax: (732)445-3134 > ******************************************* > > > > > > > > > > > From: "Pam Barker" > Subject: [Histonet] RELIA Histology Careers Bulletin Special Edition for Managers and Supervisors - 2-8-2016 > Date: February 8, 2016 at 12:44:26 PM EST > To: > > > Hi Histopeeps! > What would make the perfect management position? > ? Is it the location? > ? Perhaps the type of lab? > ? How about the size of the staff/lab? > ? Maybe it?s the Hours? The Money and/or benefits? > ? Could it be that a particular situation is the next logical step in your > career? > > More than likely the answer is all of the above in different degrees. > That is why I am contacting you with this special bulletin. I am starting > to get a lot of management opportunities and I wanted to touch base and let > you know. Your next opportunity might be just around the corner and I might > have it for you. If you are looking for a position right now please contact > me right away. We can talk about my current positions OR about a customized > search on your behalf. If you aren?t looking right away but want to let me > know what would make a perfect job for you so that I could keep an eye out, > that would be great too. To do that just shoot me an email at > relia1 at earthlink.net or call me toll free at 866-607-3542. > > Here is a list of my current managerial opportunities: > Lab Manager ? Williamsburg, VA > Pathology Supervisor ? Manchester, NH > Histology Supervisor ? Amarillo, TX > Lead Tech/Supervisor ? Fayetteville, AR > Sr. Clinical Lab Specialist/Pathology ? Norfolk, VA > IHC Tech Support ? Los Angeles, CA > > All of these clients offer autonomy in your position and a staff eager to > welcome their new manager. > > I really appreciate you taking time out of your busy day to read my e-mail > Thanks Again! > > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > Pam M. Barker > > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1 at earthlink.net > www.facebook.com/PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abadesuyi at nrh-ok.com Tue Feb 9 15:47:23 2016 From: abadesuyi at nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Tue, 9 Feb 2016 15:47:23 -0600 Subject: [Histonet] Slide and Cassette Labelers Message-ID: <04EE4F75BB5FB246ADB68D69B7460443A405DDA1B4@MAIL.nrhnt.nrh-ok.com> Hi Guys, I hope you guys are doing great. Our lab is in the process of buying Slide and Cassette Labelers and I will really appreciate inputs from you guys. Best regards, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 abadesuyi at nrh-ok.com ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From Fawn.Bomar at HalifaxRegional.com Wed Feb 10 04:29:52 2016 From: Fawn.Bomar at HalifaxRegional.com (Fawn Bomar) Date: Wed, 10 Feb 2016 10:29:52 +0000 Subject: [Histonet] Competency Message-ID: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA1A0ABB@EXCH-2K10.hrhs.com> Hi to everyone yet once again! I am sorry to bother you all with yet another question, but I was wondering if anyone had a competency/orientation checklist specifically for team leaders/ Supervisors. I received many responses and great competency checklists from the group for the histotech position itself and managed to make one that fit our lab using tips from all of them. I can't thank you all enough for all your help and for sharing all your knowledge with everyone! Thank you again, Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From LBUSTAMANTE at cvm.tamu.edu Wed Feb 10 11:09:21 2016 From: LBUSTAMANTE at cvm.tamu.edu (Bustamante, Lin) Date: Wed, 10 Feb 2016 17:09:21 +0000 Subject: [Histonet] NSH Continue education award Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC90158130101@CVMMB02.cvm.tamu.edu> I would like to nominate a student I have. Can someone guide me what to do next? Thank you very much. Lin. Lin S. Bustamante B.Sc. H.T.(ASCP) Research Associate Texas A&M University College of Veterinay Medicine VIBS Histology Laboratory Supervisor Room 107 VMA College Station, Texas 77843-4458 (979)845-3177 (979)458-3499 Fax From wbenton at cua.md Wed Feb 10 11:28:56 2016 From: wbenton at cua.md (Walter Benton) Date: Wed, 10 Feb 2016 17:28:56 +0000 Subject: [Histonet] NSH Continue education award In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC90158130101@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC90158130101@CVMMB02.cvm.tamu.edu> Message-ID: <557321c8a8e34d98ad7388a91e21004a@MAIL01.GCU-MD.local> http://www.nsh.org/content/scholarships -----Original Message----- From: Bustamante, Lin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, February 10, 2016 12:09 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] NSH Continue education award I would like to nominate a student I have. Can someone guide me what to do next? Thank you very much. Lin. Lin S. Bustamante B.Sc. H.T.(ASCP) Research Associate Texas A&M University College of Veterinay Medicine VIBS Histology Laboratory Supervisor Room 107 VMA College Station, Texas 77843-4458 (979)845-3177 (979)458-3499 Fax _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From liz at premierlab.com Wed Feb 10 12:00:22 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Wed, 10 Feb 2016 11:00:22 -0700 Subject: [Histonet] NSH Continue education award In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC90158130101@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC90158130101@CVMMB02.cvm.tamu.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE02854EF54219@SBS2K8.premierlab.local> Lin The link to nominate one of your students for one of the NSH Student Scholarship is below. Deadline for nomination is March 1, 2016. We have tried to make the process easier this year for the program directors/education coordinators the application process consists of two fillable forms that can be downloaded from the NSH website. There are a few requirements for these awards the individual must be a student member of NSH and they need to be enrolled in a formal program. http://www.nsh.org/content/student-scholarships-1 If you have an individual who is not enrolled in a formal program then my suggestion would be to nominate them for one of the professional scholarships that NSH has to offer. In order to be eligible for these scholarships they need to be an NSH member. The deadline for nomination for these scholarships is June 1, 2016. The link is below. http://www.nsh.org/content/professional-scholarships So while I have everyone's attention I will be providing a shameless plug for the rest of the NSH Awards and Scholarships. I'm sure you have heard me say over and over again how great it is that NSH can provide so many awards and scholarships to its members. It's not really that hard to nominate yourself or one of you colleagues for an award, the NSH office and the Awards Committee has worked on trying to simplify the process. Please take the time to nominate an individual or a lab that you think has raised the bar for one of the many awards and scholarships. If you need some help please e-mail me and I would be more than willing to work with you through the process. Thanks Liz - NSH Awards Chair Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Bustamante, Lin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, February 10, 2016 10:09 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] NSH Continue education award I would like to nominate a student I have. Can someone guide me what to do next? Thank you very much. Lin. Lin S. Bustamante B.Sc. H.T.(ASCP) Research Associate Texas A&M University College of Veterinay Medicine VIBS Histology Laboratory Supervisor Room 107 VMA College Station, Texas 77843-4458 (979)845-3177 (979)458-3499 Fax _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren at gmail.com Wed Feb 10 12:29:56 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 10 Feb 2016 12:29:56 -0600 Subject: [Histonet] Job in Chicago? Message-ID: Anyone know whose lab is looking for a temp in Chicago? I've been contacted by a couple of temp agencies about an assignment in Chicago, but I've been enjoying working as an independent contractor for a while now. So if anyone is in Chicago, and your lab is the one looking for a temp, please feel free to respond or share my email with your Lab Manager. Thanks, Jay A. Lundgren, M.S., HTL (ASCP) From fdonrussello at gmail.com Wed Feb 10 12:37:15 2016 From: fdonrussello at gmail.com (Felicia DonRussello) Date: Wed, 10 Feb 2016 10:37:15 -0800 Subject: [Histonet] Cornea Message-ID: Good morning. I have a researcher that wants photographic sections of cornea (either dog or cat) and we have sectioned the cornea many times for him and they still have wrinkles. Does anyone have a trick, technique or magic spell that they have found to work? Thank you! From mundayscott at gmavt.net Wed Feb 10 12:40:52 2016 From: mundayscott at gmavt.net (scott munday) Date: Wed, 10 Feb 2016 13:40:52 -0500 (EST) Subject: [Histonet] Broken Microscope? Message-ID: <687002861.117950816.1455129652835.JavaMail.zimbra@gmavt.net> Is your lab in need of a new microscope? We have several Olympus BX40 and BX41 compound microscopes in stock! and offer a 1 year warranty on all scopes. All microscopes are fully refurbished and are priced at 40% off list. The scopes are serviced by an Authorized Olympus Service Tech before sold. Please Email or call with questions. Chad Potts 919-775-5596 From tbraud at holyredeemer.com Wed Feb 10 13:17:17 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 10 Feb 2016 19:17:17 +0000 Subject: [Histonet] slide, cassette printers Message-ID: <48E053DDF6CE074DB6A7414BA05403F806001A@HRHEX02-HOS.holyredeemer.local> We LOVE our Leica printers. IPS and IPC. We've been running them for over 8 years with few problems and little down time. We recently interfaced them to CoPath and it went very quickly and smooth. Now we are loving them more than ever. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 2. Slide and Cassette Labelers (Adesupo, Adesuyi (Banjo)) Message: 2 Date: Tue, 9 Feb 2016 15:47:23 -0600 From: "Adesupo, Adesuyi (Banjo)" Subject: [Histonet] Slide and Cassette Labelers Hi Guys, I hope you guys are doing great. Our lab is in the process of buying Slide and Cassette Labelers and I will really appreciate inputs from you guys Best regards, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 abadesuyi at nrh-ok.com ******************************** From liz at premierlab.com Wed Feb 10 14:09:48 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Wed, 10 Feb 2016 13:09:48 -0700 Subject: [Histonet] Letters of Recommendation for NSH Awards Message-ID: <14E2C6176416974295479C64A11CB9AE02854EF5421E@SBS2K8.premierlab.local> Hello Everyone I have decided to post this since I have received some e-mails back regarding the awards process. I have listed below my thoughts on Letters of Recommendation. Tips to Writing an Excellent Letter of Recommendation The Awards Committee has to sift through multiple nominations in order to choose the appropriate person for a particular award or scholarship. We receive the persons CV (if they choose to submit, it's not required for some of the awards/scholarships, but I would suggest that if you have one you should submit it with the application) some info on the nominee, but what really makes one individual stand out from the rest? It's their letters of recommendations, so here are a few tips: 1. Get focused - understand what award or scholarship you are nominating them for and focus in on the description and criteria listed on the NSH website. 2. Get personal - what has that person accomplished? Don't generalize, we don't just want to know that they are a great histotech, we know that, that's why they have been nominated we want to hear WHAT makes them a great histotech, tell us what they have done, give us some examples, what makes this person stand out amongst others, the more personal the better. 3. Get multiple people to write letters - everyone has different experiences and different viewpoints. Bottom line the more information the better. 4. And last of all - Get busy and nominate!!! The deadline for nominations is June 1st. http://www.nsh.org/content/nsh-awards-and-scholarships Thanks Liz - NSH Awards Chair Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From naje1972 at yahoo.com Wed Feb 10 14:52:23 2016 From: naje1972 at yahoo.com (cynthia haynes) Date: Wed, 10 Feb 2016 20:52:23 +0000 (UTC) Subject: [Histonet] Labelers for slides and paraffin blocks References: <683055412.2282656.1455137543349.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <683055412.2282656.1455137543349.JavaMail.yahoo@mail.yahoo.com> Vendors are welcome to contact me at naje1972 at yahoo.com. I need pricing information as well .?Cynthia Haynes-James H.T? Sent from Yahoo Mail on Android From Jeffrey.Browning at uhsystem.com Thu Feb 11 15:14:06 2016 From: Jeffrey.Browning at uhsystem.com (Browning, Jeffrey) Date: Thu, 11 Feb 2016 21:14:06 +0000 Subject: [Histonet] EM Tech position in Shreveport, LA Message-ID: <552bbf36b37f423598308ea1c08f7771@UHS-EXCHMB1.UHSYSTEM.ORG> University Health Shreveport in Shreveport, LA is currently recruiting for an Electron Microscopy Technician. Experience preferred but not required. Apply at https://careers.uhsystem.com/job_app_shreveport/application.aspx or inquire at jeffrey.browning at uhsystem.com. Thanks! Confidentiality Notice: This e-mail message from University Health System, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From relia1 at earthlink.net Fri Feb 12 07:04:30 2016 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 12 Feb 2016 08:04:30 -0500 Subject: [Histonet] RELIA Histology Bulletin 2-10-2016 An updated list of Spring Histology Meetings and some SWEET opportunities Message-ID: <004201d16595$ea3af580$beb0e080$@earthlink.net> Hi Histopeeps! Valentine?s Day is right around the corner and I thought I would drop you a line about some really SWEET opportunities. And I also wanted to pass along the info on some more of the state and regional meetings. The annual symposium, the state society and regional meetings are fun and educational. They give you the opportunity to network with your peers and hang out and have fun with other histotechs. Please scroll down for my updated list of state and regional meetings For further information or information on other areas check the NSH website: www.nsh.org I also have some exciting job opportunities. Please check them out! *All of these positions are full time and permanent.* *Our clients offer excellent compensation and benefits.* *Relocation and sign on bonuses are also available with many of our clients.* Here Is A List Of Our Most Exciting Job Opportunities. Lab Manager ? Williamsburg, VA Histology Supervisor ? Amarillo, TX Lead Dermpath Histotech ? Fayetteville, AR Histotech ? Milwaukee, WI- DAY shift Histotechnician All Shifts 15K sign on bonus Norfolk, VA Histology Techs ? Little Rock, AR ?DAY shift Dermpath Histotech Kansas City, KS ? DAY shift IHC Tech Support ? Los Angeles, CA ? DAY shift Histotech Louisville, KY 1st and 3rd shift available For more information on any of these opportunities please contact me at relia1 at earthlink.net or toll free at 866-607-3542 or on my cell at 407-353-5070. If you are looking for a new opportunity in another area shoot me an email I can be on the lookout for you!! ? relia1 at earthlink.net Here is the updated list of Spring Meetings: ? North Carolina: The North Carolina Society of Histotechnology meeting will be held Thursday, April 7th through Saturday, April 9th at the: DoubleTree by Hilton Raleigh-Durham Airport 4810 Page Creek Lane Research Triangle Park, North Carolina 27709 RESERVATIONS 919-941-6000, 1-800-445-8667, Fax 919-941-4845 ? Massachusetts: Saturday April 2, 2015 Annual MASH Symposium will be held at the John F. Kennedy Library and Museum in Boston, MA ? Ohio: Histology Society of Ohio Symposium/Convention April 15-16, 2016 Holiday Inn Columbus-Worthington Worthington, OH www.ohiohistology.org For additional information contact: Amy Aulthouse, Program Chair for HSO email: a-aulthouse at onu.edu ? New York: New York State Histotechnological Society?s Annual Spring Meeting ?SPECTRUM OF KNOWLEDGE? Friday April 22nd and Saturday April 23rd, 2016 Holiday Inn Express and Suites in Latham, New York Check our website for updates: www.nyhisto.com ? Indiana: Indiana Society for Histotechnology Spring Symposium EMERGING TECHNOLOGIES The Future of Histotechnology March 4th & 5th, 2016 The Wellington Fishers Banquet and Conference Center (Formerly known as the Fishers conference center) 9775 North by Northeast Blvd. Fishers, IN 46037 http://thewellingtonfishers.com/ ? Kentucky: Kentucky Society for Histotechnology 40thAnnual Symposium February 26 and 27, 2016 The Galt House 140 North 4th Street, Louisville, KY 40202 (502) 589-5200 ? Region 1: The Region 1 meeting is being held April 15-16 2016 at the Mystic Hilton in Mystic CT. For more information contact Clare Thornton at cthornton at dahlchase.com. ? Tri-State (MN, IA, WI): 13th Tri-State Symposium Scheduled for April 27-29, 2016 At the DoubleTree by Hilton, Cedar Rapids, IA. For more information: IA?Judi Stasko judith.stasko at ars.usda.gov/ MN?Lois Rowe Rowe.Lois at mayo.edu/ WI & Exhibitors?Dawn Schneider dawn.schneider at ministryhealth.org ? Arkansas/Missouri: First Annual Arkansas Missouri Joint Meeting Spring Meeting ? May 19-21st in Branson, MO. The meeting is being held at the Radisson Hotel in Branson, MO and is open to anyone in Histology and related or interested fields. For more information: ashnews at comcast.net ? Florida: The Florida Society for Histotechnology meeting will be May 19-22, 2016 at the St. Pete Bayfront Hilton. For more information: http://www.fshgroup.org/meeting/ ? Colorado: The 2016 CSH meeting will be held on May 13th & 14th at the Beaver Run Resort in Breckenridge, CO. For more information: www.coloradohisto.org ? Illinois: Illinois Society for Histotechnologists Education, the Pathway to Success May 19 & 20, 2016 Four Points Sheraton 319 Fountains Parkway Fairview Heights, IL 62208 (near St. Louis, MO) (618)622-9500 for more information: www.illinoishistologysociety.org ? New York: NYSHS Annual Meeting "SPECTRUM OF KNOWLEDGE" April 22-23 2016 Holiday Inn Express Suites at Latham New York http://www.nyhisto.com ? Georgia: HISTOPALOOZA! 2016 is being held at Lake Lanier Legacy Lodge, Lanier Islands, Buford, GA April 22-24, 2016. For more information: www.histosearch.com/gsh// ? Texas: The Texas Society for Histotechnology will be holding their 38th annual S/C April 29-May 1, 2016. The site for our 2016 Symposium/Convention will be The JW Marriott Hotel Houston located in Houston, Texas. For more information please visit their website at www.txsh.org ? National: The annual NSH S/C will be held in Long Beach, CA from Sept 16-21 2016. For more information: www.histoconvention.org Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From criley at dpspa.com Fri Feb 12 09:18:32 2016 From: criley at dpspa.com (Charles Riley) Date: Fri, 12 Feb 2016 10:18:32 -0500 Subject: [Histonet] Fwd: Natalia's Credentials In-Reply-To: References: Message-ID: My lab is looking to hire a tech who has been trained in another country. Management wants to know if their credentials qualify them to work in a CAP accredited lab without direct supervision or if they will need to sit for the ASCP board exam. If they need to sit for the exam do her credentials qualify her to take the exam now? Her credentials are listed below Ministry of Public Health & Social Care Diploma Graduated the full course of education at Institute of Health Care Employees with Secondary Professional Education Bulgarian Medical Academy. Qualification of Clinical Laboratory Assistant Transcript includes: Laboratory Equipment and Laboratory Appliances Clinical Laboratory Histology with General Pathology & Histological Technique Biochemistry 120 hours of Histological Laboratory -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE From Timothy.Morken at ucsf.edu Fri Feb 12 09:49:09 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 12 Feb 2016 15:49:09 +0000 Subject: [Histonet] Fwd: Natalia's Credentials In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF6FD168DD@ex07.net.ucsf.edu> Charles, Foreign course work is useless for CLIA until it is vetted by a transcript service that can evaluate the validity of the school and how that course work matches with the required typical US courses. ASCP requires such vetting for foreign applications for ASCP certification (I've been involved with a few people taking that route). It can take weeks to months to get such verification depending on the bureaucracy involved in the foreign country. And the applicant has to pay for it. Experience counts, but you don't know what the quality of that is either until you see her in action. If this person is already in the US and local to you, and you still want to pursue it, I suggest hiring her as a lab assistant with the idea you would evaluate her skills on site, if you are willing to do that. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, February 12, 2016 7:19 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fwd: Natalia's Credentials My lab is looking to hire a tech who has been trained in another country. Management wants to know if their credentials qualify them to work in a CAP accredited lab without direct supervision or if they will need to sit for the ASCP board exam. If they need to sit for the exam do her credentials qualify her to take the exam now? Her credentials are listed below Ministry of Public Health & Social Care Diploma Graduated the full course of education at Institute of Health Care Employees with Secondary Professional Education Bulgarian Medical Academy. Qualification of Clinical Laboratory Assistant Transcript includes: Laboratory Equipment and Laboratory Appliances Clinical Laboratory Histology with General Pathology & Histological Technique Biochemistry 120 hours of Histological Laboratory -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rgonzalez at centraltexasgi.com Fri Feb 12 11:05:14 2016 From: rgonzalez at centraltexasgi.com (Rayen Gonzalez) Date: Fri, 12 Feb 2016 17:05:14 +0000 Subject: [Histonet] Need Slide Cabinets Message-ID: <0a9da3075e1940f081b052fcd22bd1d6@EXCHANGE.CTGI.local> Hello all! Our lab is in need for STACKABLE METAL SLIDE CABINETS. I would like to know which vendors supply this product and if you can send me a quote? Or if your lab is content with the slide cabinets you use, if you can give me your vendors information? Thank you, Rayen Gonzalez B.S., HT (ASCP) From wbenton at cua.md Fri Feb 12 11:39:03 2016 From: wbenton at cua.md (Walter Benton) Date: Fri, 12 Feb 2016 17:39:03 +0000 Subject: [Histonet] Need Slide Cabinets In-Reply-To: <0a9da3075e1940f081b052fcd22bd1d6@EXCHANGE.CTGI.local> References: <0a9da3075e1940f081b052fcd22bd1d6@EXCHANGE.CTGI.local> Message-ID: <8d0846c9b5bc465d9a7ca917d9732238@MAIL01.GCU-MD.local> http://www.phoenixmetalproducts.com/ -----Original Message----- From: Rayen Gonzalez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, February 12, 2016 12:05 PM To: histonet at lists.utsouthwestern.edu Cc: Lin Bustamante Subject: [Histonet] Need Slide Cabinets Hello all! Our lab is in need for STACKABLE METAL SLIDE CABINETS. I would like to know which vendors supply this product and if you can send me a quote? Or if your lab is content with the slide cabinets you use, if you can give me your vendors information? Thank you, Rayen Gonzalez B.S., HT (ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From LRaff at uropartners.com Fri Feb 12 12:15:30 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 12 Feb 2016 18:15:30 +0000 Subject: [Histonet] Need Slide Cabinets--Response and post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B6AADEB@COLOEXCH01.uropartners.local> We buy our slide cabinets directly from the manufacturer Phoenix Metal Products. You can contact them at 516-546-4200. Buying direct is cheaper than going through a distributor. Unrelated blog post : http://www.chicagonow.com/downsize-maybe/2016/02/timeshare-hat-trick-how-we-wound-up-with-a-house-a-hole-and-a-week/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From MAUGER at email.chop.edu Fri Feb 12 12:31:35 2016 From: MAUGER at email.chop.edu (Mauger, Joanne) Date: Fri, 12 Feb 2016 18:31:35 +0000 Subject: [Histonet] HCV antibody Message-ID: <569ede8b64b04110aec817797db81f98@EXCMBXPW17.chop.edu> Hello All, Which HCV antibody is everyone using for FFPE, human tissue?? Thanks, Jo Mauger Children's Hospital of Philadelphia _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock at mclean.harvard.edu Fri Feb 12 12:56:47 2016 From: twheelock at mclean.harvard.edu (Wheelock, Timothy R.) Date: Fri, 12 Feb 2016 18:56:47 +0000 Subject: [Histonet] Microscopic slide files Message-ID: <69718C0B0B3C414D9F8E7214AD400CC99FB4B6E1@PHSX10MB11.partners.org> Hi Rayen: We buy our stackable slide files and slide file base from Fisher Scientific: Here are the items and catalogue numbers, which are for tan-colored files. Other colors are available. Microscopic slide file drawers 07-212-100 Microscopic slide file drawer base 07-212-104 Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From tbraud at holyredeemer.com Fri Feb 12 13:49:57 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 12 Feb 2016 19:49:57 +0000 Subject: [Histonet] Credentials Message-ID: <48E053DDF6CE074DB6A7414BA05403F80606FF@HRHEX02-HOS.holyredeemer.local> There is no CAP requirement that a person has to be certified or qualified in any way to perform Histology or Histology Assistant duties. As long as you can document their proven competency, they are good to perform any procedure. The only exception in the Histology lab, is if that tech is required to perform gross. Then they must be documented to be able to perform CLIAA high complexity testing. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 From jclark at pcnm.com Fri Feb 12 14:40:32 2016 From: jclark at pcnm.com (Joanne Clark) Date: Fri, 12 Feb 2016 20:40:32 +0000 Subject: [Histonet] Foreign Trained Tech In-Reply-To: References: Message-ID: <7A7BDD92B984E847A7E71BC9C00A66D3126DEF56@S11MAILD034N2.sh11.lan> Tim is absolutely right. I am a Canadian trained tech and before I could work in the US I had my credentials evaluated by WES (you can google them to get contact info) to see which ASCP exam I qualified for. Joanne Clark, BAAS, HT Director of Histology Pathology Consultants of New Mexico ------------------------------ Message: 3 Date: Fri, 12 Feb 2016 10:18:32 -0500 From: Charles Riley To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fwd: Natalia's Credentials Message-ID: Content-Type: text/plain; charset=UTF-8 My lab is looking to hire a tech who has been trained in another country. Management wants to know if their credentials qualify them to work in a CAP accredited lab without direct supervision or if they will need to sit for the ASCP board exam. If they need to sit for the exam do her credentials qualify her to take the exam now? Her credentials are listed below Ministry of Public Health & Social Care Diploma Graduated the full course of education at Institute of Health Care Employees with Secondary Professional Education Bulgarian Medical Academy. Qualification of Clinical Laboratory Assistant Transcript includes: Laboratory Equipment and Laboratory Appliances Clinical Laboratory Histology with General Pathology & Histological Technique Biochemistry 120 hours of Histological Laboratory -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE ------------------------------ Message: 4 Date: Fri, 12 Feb 2016 15:49:09 +0000 From: "Morken, Timothy" To: Charles Riley Cc: Histonet Subject: Re: [Histonet] Fwd: Natalia's Credentials Message-ID: <761E2B5697F795489C8710BCC72141FF6FD168DD at ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Charles, Foreign course work is useless for CLIA until it is vetted by a transcript service that can evaluate the validity of the school and how that course work matches with the required typical US courses. ASCP requires such vetting for foreign applications for ASCP certification (I've been involved with a few people taking that route). It can take weeks to months to get such verification depending on the bureaucracy involved in the foreign country. And the applicant has to pay for it. Experience counts, but you don't know what the quality of that is either until you see her in action. If this person is already in the US and local to you, and you still want to pursue it, I suggest hiring her as a lab assistant with the idea you would evaluate her skills on site, if you are willing to do that. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, February 12, 2016 7:19 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fwd: Natalia's Credentials My lab is looking to hire a tech who has been trained in another country. Management wants to know if their credentials qualify them to work in a CAP accredited lab without direct supervision or if they will need to sit for the ASCP board exam. If they need to sit for the exam do her credentials qualify her to take the exam now? Her credentials are listed below Ministry of Public Health & Social Care Diploma Graduated the full course of education at Institute of Health Care Employees with Secondary Professional Education Bulgarian Medical Academy. Qualification of Clinical Laboratory Assistant Transcript includes: Laboratory Equipment and Laboratory Appliances Clinical Laboratory Histology with General Pathology & Histological Technique Biochemistry 120 hours of Histological Laboratory -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://cp.mcafee.com/d/FZsS938OrhovvpKyqemkPtPqdQnCnNPUVYSzt5VBYsUCyrhKyYO-ev7e6QrLIe8IcTd79IhIvIiEamJ1nBPqREaYKrIC-DV1N_HYUMCyeovWZOWqdNT8IIICzBxZPG8FHnjlLtPBgY-F6lK1FJ4srjKrLOqbaab0VZx4TsS03fBiterDiHsOpzWj-ceZ1L1dnoovaAVgtHxel9R1FIDaAWwQKgGT2TQ1hYGjFPsWlrCjcvivNxTEdCQhRPhOCr2YGjG3jh07i8_io1Cy0cRfd42b0U0Ph06QETVEwhjdII6WlYMYvib5NlI ------------------------------ Message: 5 Date: Fri, 12 Feb 2016 17:05:14 +0000 From: Rayen Gonzalez To: "histonet at lists.utsouthwestern.edu" Cc: Lin Bustamante Subject: [Histonet] Need Slide Cabinets Message-ID: <0a9da3075e1940f081b052fcd22bd1d6 at EXCHANGE.CTGI.local> Content-Type: text/plain; charset="us-ascii" Hello all! Our lab is in need for STACKABLE METAL SLIDE CABINETS. I would like to know which vendors supply this product and if you can send me a quote? Or if your lab is content with the slide cabinets you use, if you can give me your vendors information? Thank you, Rayen Gonzalez B.S., HT (ASCP) ------------------------------ Message: 6 Date: Fri, 12 Feb 2016 17:39:03 +0000 From: Walter Benton To: Rayen Gonzalez , "histonet at lists.utsouthwestern.edu" Cc: Lin Bustamante Subject: Re: [Histonet] Need Slide Cabinets Message-ID: <8d0846c9b5bc465d9a7ca917d9732238 at MAIL01.GCU-MD.local> Content-Type: text/plain; charset="us-ascii" http://cp.mcafee.com/d/5fHCNEg3zqb3XXdQjhOOCrKrhKyYO-ev7fCQrELcLzD4QjqdQnCnNPUVMSztZxN5xCVEVdydzZyl1iREaYKrmJ1nBPtATQ_8efZvD64QhP3_nKnjhKeV5BBAQsIfKth5dqWqJXKsG7DR8OJMddEL6QXCXYCyOyyMevohdTdw0Bytvx8fl-uDHhGwTqtenMSjBitgqn8lrxrW0E-l9QVKtaJP9CfFfUMXQ6Pq8WVEVjdxul9R1FEw3F4vFc0Ph06qDCy15ws0pEw3qkrYQg8FCSm3onSW -----Original Message----- From: Rayen Gonzalez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, February 12, 2016 12:05 PM To: histonet at lists.utsouthwestern.edu Cc: Lin Bustamante Subject: [Histonet] Need Slide Cabinets Hello all! Our lab is in need for STACKABLE METAL SLIDE CABINETS. I would like to know which vendors supply this product and if you can send me a quote? Or if your lab is content with the slide cabinets you use, if you can give me your vendors information? Thank you, Rayen Gonzalez B.S., HT (ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://cp.mcafee.com/d/FZsScygAd1NJ5xZZCW9EVpjdTdEThupv7fzDPqdQnCnNPyq9J6WbPbUVYsUrhK-MUyMPsQsCN6N-NawFqQ5undHmwHOVKOrWvA77-LPz2q8Vx_HTbFET7syOOOqem7TeEyCJtdmZTel3PWApmU6CQjhOrjKrLOqbaab0VZx4TsS03fBiterDiHsOpzWj-ceZ1L1dnoovaAVgtHxel9R1FIDaAWwQKgGT2TQ1hYGjFPsWlrCjcvivNxTEdCQhRPhOCr2YGjG3jh07i8_io1Cy0cRfd42b0U0Ph06QETVEwhjdII6OmA2 CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://cp.mcafee.com/d/2DRPoQrhovvpKyqemkPtPqdQnCnNPUVYSzt5VBYsUCyrhKyYO-ev7e6QrLIe8IcTd79IhIvIiEamJ1nBPqREaYKrIC-DV1N_HYUMCyeovWZOWqdNT8IIICzBxZPG8FHnjlLtPBgY-F6lK1FJeVJeVK_9EIEEI3DS4jtPo0c-l9QVKtaJP9CfFfUMXQ6Y4RtxxYGjB1SK4VkDk6COsGjG3iV2Hsbvg57OFeDdPFlKpcNZ9_67uwSrh7nd7apIbOFeEdd40t8zZ9w6q80PkYQg8I3w3d40rizvCy15cSOMryrTK ------------------------------ End of Histonet Digest, Vol 147, Issue 12 ***************************************** Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From lilainouye at msn.com Fri Feb 12 15:14:31 2016 From: lilainouye at msn.com (lila inouye) Date: Fri, 12 Feb 2016 15:14:31 -0600 Subject: [Histonet] FW: GI Pathologist Consulting In-Reply-To: <1811510467.142570.1454380259536.JavaMail.yahoo@mail.yahoo.com> References: , <1811510467.142570.1454380259536.JavaMail.yahoo@mail.yahoo.com> Message-ID: Hello Histonet, I am new to Histonet so please excuse any faux pas I make in trying to reply to Adrienne Anderson's e-mail. I could not find a way to reply to Adrienne directly. Hi Adrienne, I am a pathologist in Dallas who has considerable experience in GI pathology. I would be interested in doing the project with the company you work for. I would like to learn more about the position. If you prefer to contact me directly my personal e-mail is LilaInouye at msn.com and my cell phone is (414) 305-3908. I look forward to talking with you, Lila Inouye, M.D. Date: Tue, 2 Feb 2016 02:30:59 +0000 From: quinerva at yahoo.com To: lilainouye at msn.com Subject: Fw: [Histonet] GI Pathologist Consulting Hi Lila! Here's a proposal for some pathology work, viewed on my HistoNet postings. FYI, in case you are interested.Jo ----- Forwarded Message ----- From: Adrienne Anderson via Histonet To: histonet at lists.utsouthwestern.edu Sent: Monday, February 1, 2016 1:26 PM Subject: [Histonet] GI Pathologist Consulting Hello all, I was wondering if anyone knows of a GI pathologist interested in doing a quick side project as a consultant for the company I work for? If so, please message me privately and I can give you the details. Thanks so much, Adrienne Anderson _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Fri Feb 12 15:36:22 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 12 Feb 2016 21:36:22 +0000 (UTC) Subject: [Histonet] Credentials In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F80606FF@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F80606FF@HRHEX02-HOS.holyredeemer.local> Message-ID: <909405323.2768197.1455312982629.JavaMail.yahoo@mail.yahoo.com> Yes, BUT, in states where a certification/license is required to work in Histology (such as Florida) state requirements supersede CAP's and the inspectors have to oblige.Ren?? On Friday, February 12, 2016 3:19 PM, Terri Braud via Histonet wrote: There is no CAP requirement that a person has to be certified or qualified in any way to perform Histology or Histology Assistant duties. As long as you can document their proven competency, they are good to perform any procedure.? The only exception in the Histology lab,? is if that tech is required to perform gross.? Then they must be documented to be able to perform CLIAA high complexity testing. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From yesyes at comcast.net Fri Feb 12 20:24:53 2016 From: yesyes at comcast.net (yesyes at comcast.net) Date: Sat, 13 Feb 2016 02:24:53 +0000 (UTC) Subject: [Histonet] Credentials Message-ID: <921226170.2767105.1455330293801.JavaMail.zimbra@comcast.net> From KSimeone at leavittmgt.com Mon Feb 15 10:16:55 2016 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Mon, 15 Feb 2016 16:16:55 +0000 Subject: [Histonet] TCR alpha/beta gamma/delta Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CB902C8E5@vm-email.leavittmgt.com> Is anyone out in Histoland performing TCR for alpha/beta & gamma/delta via IHC on the Leica Bond? If so, mind sharing your protocols and vendor information with me? Pretty please? Looking to bring this on. I appreciate it in advance. Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor Delray Beach Technical Laboratory ADCS Clinics, LLC ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From DKnutson at primecare.org Mon Feb 15 12:14:06 2016 From: DKnutson at primecare.org (Knutson, Deanne) Date: Mon, 15 Feb 2016 12:14:06 -0600 Subject: [Histonet] (CLRW) - Clinical Laboratory Reagent Water Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A4D5970FFC@EXCHANGE2K7.staprimecare.org> Fellow Histonetters, Our medical center will no longer be providing us with water for our laboratory procedures. I was wondering where other labs who need to purchase Clinical Laboratory Reagent Water are doing so? Thank you for your help! Deanne Knutson Supervisor Anatomic Pathology ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From jvickroy at SpringfieldClinic.com Mon Feb 15 13:12:06 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Mon, 15 Feb 2016 19:12:06 +0000 Subject: [Histonet] Tissue cassette baskets for VIP 6 Message-ID: <9B1A1501A800064397369BD8072E6BCA06502BD8@E2K10DB.springfieldclinic.com> Anybody have an idea where we can get a used cassette basket that will fit in the VIP 6? The cost of a new one is pretty high? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From Valerie.Hannen at parrishmed.com Tue Feb 16 09:28:59 2016 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Tue, 16 Feb 2016 10:28:59 -0500 Subject: [Histonet] MICROTOME KNIFE SHARPENING Message-ID: <450B7A81EDA0C54E97C53D60F00776C323546750BC@isexstore03> Hi all... Hoping you might be answer a couple of questions that I have. 1) Does anyone use a microtome knife sharpening kit made by Pathco? If so, 2) How well does it sharpen the blades? 3) Is there any issue on attaching the abrasive sheets to the honing plate? 4) Do the abrasive sheets tend to "move" during the sharpening process, that would cause the blade not be on them but on the plate instead? I currently am using coarse and fine abrasive liquids along with the honing plate... but the coarse, fine and honing liquids are becoming quite expensive and hard to come by. Any and all replies are welcomed. Thank you in advance, Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From Timothy.Morken at ucsf.edu Tue Feb 16 10:05:12 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 16 Feb 2016 16:05:12 +0000 Subject: [Histonet] (CLRW) - Clinical Laboratory Reagent Water In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F76833A4D5970FFC@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F76833A4D5970FFC@EXCHANGE2K7.staprimecare.org> Message-ID: <761E2B5697F795489C8710BCC72141FF6FD17480@ex07.net.ucsf.edu> Deanne, If you just need it for a few critical reagents You can get type 1 water by the pint or the gallon from Fisher (NERL Type 1 water). It's probably overkill for most histology procedures, but convenient if you need it. Note that the expiry clock of 30 days starts when you open the bottle. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Knutson, Deanne via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, February 15, 2016 10:14 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] (CLRW) - Clinical Laboratory Reagent Water Fellow Histonetters, Our medical center will no longer be providing us with water for our laboratory procedures. I was wondering where other labs who need to purchase Clinical Laboratory Reagent Water are doing so? Thank you for your help! Deanne Knutson Supervisor Anatomic Pathology ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jvickroy at SpringfieldClinic.com Tue Feb 16 11:10:40 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Tue, 16 Feb 2016 17:10:40 +0000 Subject: [Histonet] Nuclear Bubbling Message-ID: <9B1A1501A800064397369BD8072E6BCA06503B35@E2K10DB.springfieldclinic.com> Struggling to find an answer. We do a lot of GI biopsies in our lab. Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense. Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times. I do not find that the problem is fixation. In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend). There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining. I really thought that this might be the issue however I'm not sure at this point. Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without. I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue. Here are my questions: 1. Could it be something that is happening with the tissue before it gets to the lab? Usually a delay if fixation causes other artifacts but not bubbling. Could it be heat from the GI procedure? 2. We do use blue sponges for our biopsies. I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3. What about the heat stage in our Prisma stainer? I am really getting frustrated. Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling". Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue. I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From jerrysedgewick at gmail.com Tue Feb 16 11:31:27 2016 From: jerrysedgewick at gmail.com (J. Sedgewick) Date: Tue, 16 Feb 2016 11:31:27 -0600 Subject: [Histonet] Complementary webinar on post-processing scientific images, Feb 24 @ 1PM EST In-Reply-To: References: Message-ID: <1557B579CB2A478B884FD20B9E5064D6@sedge> Hello All, Do you post-process your scientific images? Typical tools for common adjustments include Photoshop and ImageJ, but they aren?t the best answer. You are invited to attend a complementary webinar on Feb 24 at 1:00PM EST ? ?Best Practices for Post-Processing of Scientific Images? Reserve your webinar seat now at https://attendee.gotowebinar.com/register/1379379490730827010 I'll be giving this webinar. Also, a similar presentation will be given for the histology WOW 2016 with Dr. Michael Linden at the University of Minnesota on March 25. Best, Jerry Sedgewick From JJennings at thedermlab.com Tue Feb 16 12:00:59 2016 From: JJennings at thedermlab.com (Jonathan Jennings) Date: Tue, 16 Feb 2016 18:00:59 +0000 Subject: [Histonet] Sakura Pre-Processing Fixative (7117) for X120 Processors Message-ID: <248395418C8C074A83C7D06081C4F01634741ADB@DERMLAB-SBS.dermlab.local> Hello All, I manage a histology Dermpath lab (We only process skins) and we use Sakura X120 Processors. We have been doing excellent with the use of our x120 processors that allowed use to have slides on the Pathologist desk within 5 hours from the time we received the specimens. That said, there is always room for improvement! As anyone who uses the Sakura x120 Xpress processors know, all of the solutions and reagents required are "proprietary" so I don't know exactly what is in them. To top that off, we bought the processors from a 3rd party vendor, so Sakura isn't too quick to come out and help me with this. All of That noted, the reason I am sending this email is because I want to incorporate the pre-processing fixative because the specimens we receive in the afternoon haven't been in formalin as long (3-5 hours) as the specimens that we receive by mail in the morning (in formalin for 18+ hours). Our preprocessing solution is working ok, but I read in the user manual about preprocessing fixative that should be used instead, if the specimens were not completely fixed before processing. Here are a few details of our current processing workflow with only using preprocessing solution. * When grossing we have 3 small stirring hot plates set at 38 Celsius. Each stir plate described below o 1 for the 0.2 or < Biopsies filled with 10% NBF prior to preprocessing solution for 15 minutes then processed on standard (1 hour program) o 1 for the 0.3 or > Biopsies Filled with Statfix (statlab) Must be in statfix. Cassettes must be in for at least 30 minutes prior to preprocessing solution (30 minutes) Then Placed on the processor (Extended - 2 hour program) o 1 for the excisions, Cyst and all other fatty skin specimens, filled with statfix Cassettes must be in for at least 1 hour prior to preprocessing solution (30 minutes) The placed on processor (Extended - 2 hour program) I tried the preprocessing fixative (no heat, just stirring agitation) on test excisions and it definitely made the tissue more firm, (almost too hard) for 30 minutes. We had to use preprocessing solution for 1 hour on excisions, so that is a 30 minute improvement. It also turned the tissue yellow, which was weird. Before I try to test more tissue, I wanted to see if anyone else has went through the same preprocessing fixative testing with skins. Questions Finally! ;) * Does anyone use the preprocessing fixative for skin specimens and would you mind giving me some insight/advice on how to incorporate? * Is it ok to incorporate any heat to the preprocessing solution or preprocessing fixative to speed up processing time? * Does the preprocessing fixative affect IHCs any differently than the preprocessing solution? * Does anyone have any comments/ideas about my inquiry? I apologize for the lengthy post. Thank you for taking the time to read it. I hope everyone has a blessed day! This e-mail transmission, and any documents, files or previous e-mail messages attached to it, may contain confidential information. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of any of the information contained in or attached to this message is strictly prohibited. If you have received this transmission in error, please immediately notify Jonathan Jennings by reply email or by telephone 1-855-705-1776, and destroy the original transmission and its attachments without reading them or saving them to any media storage device. From rjbuesa at yahoo.com Tue Feb 16 12:12:27 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 16 Feb 2016 18:12:27 +0000 (UTC) Subject: [Histonet] Nuclear Bubbling In-Reply-To: <9B1A1501A800064397369BD8072E6BCA06503B35@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA06503B35@E2K10DB.springfieldclinic.com> Message-ID: <656599459.4038172.1455646347478.JavaMail.yahoo@mail.yahoo.com> If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain ? "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.ren? On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" wrote: Struggling to find an answer.? We do a lot of GI biopsies in our lab.? Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense.? Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times.? I do not find that the problem is fixation.? In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend).? There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining.? I really thought that this might be the issue however I'm not sure at this point.? Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without.? I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue.? Here are my questions: 1.? ? ? ? Could it be something that is happening with the tissue before it gets to the lab?? Usually a delay if fixation? causes other artifacts but not bubbling.? Could it be heat from the GI procedure? 2.? ? ? We do use blue sponges for our biopsies.? I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3.? ? ? What about the heat stage in our Prisma stainer? I am really getting frustrated.? Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling".? Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue.? I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois? 62703 Office:? 217-528-7541, Ext. 15121 Email:? jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From badams at acadianagastro.com Tue Feb 16 12:34:46 2016 From: badams at acadianagastro.com (Brent Adams) Date: Tue, 16 Feb 2016 18:34:46 +0000 Subject: [Histonet] (CLRW) Clinical Laboratory Reagent Water In-Reply-To: References: Message-ID: Deanne, I purchase 5 liter cubes from Mercedes Medical. They can provide test results on each batch of water and fax to you. I did have a CLIA inspector advise to document daily on the water as is written on the cube for clarity of water, no sediments or signs of contamination. Think 5 Liters is about $12.50 and I use a little less than two a month. Brent Adams ? BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, February 16, 2016 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 147, Issue 15 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. (CLRW) - Clinical Laboratory Reagent Water (Knutson, Deanne) 2. Tissue cassette baskets for VIP 6 (Vickroy, James) 3. MICROTOME KNIFE SHARPENING (Hannen, Valerie) 4. Re: (CLRW) - Clinical Laboratory Reagent Water (Morken, Timothy) 5. Nuclear Bubbling (Vickroy, James) 6. Complementary webinar on post-processing scientific images, Feb 24 @ 1PM EST (J. Sedgewick) ---------------------------------------------------------------------- Message: 1 Date: Mon, 15 Feb 2016 12:14:06 -0600 From: "Knutson, Deanne" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] (CLRW) - Clinical Laboratory Reagent Water Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A4D5970FFC at EXCHANGE2K7.staprimecare.org> Content-Type: text/plain; charset="us-ascii" Fellow Histonetters, Our medical center will no longer be providing us with water for our laboratory procedures. I was wondering where other labs who need to purchase Clinical Laboratory Reagent Water are doing so? Thank you for your help! Deanne Knutson Supervisor Anatomic Pathology ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ------------------------------ Message: 2 Date: Mon, 15 Feb 2016 19:12:06 +0000 From: "Vickroy, James" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Tissue cassette baskets for VIP 6 Message-ID: <9B1A1501A800064397369BD8072E6BCA06502BD8 at E2K10DB.springfieldclinic.com> Content-Type: text/plain; charset="us-ascii" Anybody have an idea where we can get a used cassette basket that will fit in the VIP 6? The cost of a new one is pretty high? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ------------------------------ Message: 3 Date: Tue, 16 Feb 2016 10:28:59 -0500 From: "Hannen, Valerie" To: "Histonet at lists.utsouthwestern.edu" Subject: [Histonet] MICROTOME KNIFE SHARPENING Message-ID: <450B7A81EDA0C54E97C53D60F00776C323546750BC at isexstore03> Content-Type: text/plain; charset="us-ascii" Hi all... Hoping you might be answer a couple of questions that I have. 1) Does anyone use a microtome knife sharpening kit made by Pathco? If so, 2) How well does it sharpen the blades? 3) Is there any issue on attaching the abrasive sheets to the honing plate? 4) Do the abrasive sheets tend to "move" during the sharpening process, that would cause the blade not be on them but on the plate instead? I currently am using coarse and fine abrasive liquids along with the honing plate... but the coarse, fine and honing liquids are becoming quite expensive and hard to come by. Any and all replies are welcomed. Thank you in advance, Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== ------------------------------ Message: 4 Date: Tue, 16 Feb 2016 16:05:12 +0000 From: "Morken, Timothy" To: "Knutson, Deanne" Cc: Histonet Subject: Re: [Histonet] (CLRW) - Clinical Laboratory Reagent Water Message-ID: <761E2B5697F795489C8710BCC72141FF6FD17480 at ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Deanne, If you just need it for a few critical reagents You can get type 1 water by the pint or the gallon from Fisher (NERL Type 1 water). It's probably overkill for most histology procedures, but convenient if you need it. Note that the expiry clock of 30 days starts when you open the bottle. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Knutson, Deanne via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, February 15, 2016 10:14 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] (CLRW) - Clinical Laboratory Reagent Water Fellow Histonetters, Our medical center will no longer be providing us with water for our laboratory procedures. I was wondering where other labs who need to purchase Clinical Laboratory Reagent Water are doing so? Thank you for your help! Deanne Knutson Supervisor Anatomic Pathology ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 16 Feb 2016 17:10:40 +0000 From: "Vickroy, James" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Nuclear Bubbling Message-ID: <9B1A1501A800064397369BD8072E6BCA06503B35 at E2K10DB.springfieldclinic.com> Content-Type: text/plain; charset="us-ascii" Struggling to find an answer. We do a lot of GI biopsies in our lab. Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense. Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times. I do not find that the problem is fixation. In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend). There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining. I really thought that this might be the issue however I'm not sure at this point. Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without. I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue. Here are my questions: 1. Could it be something that is happening with the tissue before it gets to the lab? Usually a delay if fixation causes other artifacts but not bubbling. Could it be heat from the GI procedure? 2. We do use blue sponges for our biopsies. I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3. What about the heat stage in our Prisma stainer? I am really getting frustrated. Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling". Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue. I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ------------------------------ Message: 6 Date: Tue, 16 Feb 2016 11:31:27 -0600 From: "J. Sedgewick" To: Subject: [Histonet] Complementary webinar on post-processing scientific images, Feb 24 @ 1PM EST Message-ID: <1557B579CB2A478B884FD20B9E5064D6 at sedge> Content-Type: text/plain; format=flowed; charset="UTF-8"; reply-type=original Hello All, Do you post-process your scientific images? Typical tools for common adjustments include Photoshop and ImageJ, but they aren?t the best answer. You are invited to attend a complementary webinar on Feb 24 at 1:00PM EST ? ?Best Practices for Post-Processing of Scientific Images? Reserve your webinar seat now at https://attendee.gotowebinar.com/register/1379379490730827010 I'll be giving this webinar. Also, a similar presentation will be given for the histology WOW 2016 with Dr. Michael Linden at the University of Minnesota on March 25. Best, Jerry Sedgewick ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 147, Issue 15 ***************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. From JMacDonald at mtsac.edu Tue Feb 16 13:33:47 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Tue, 16 Feb 2016 11:33:47 -0800 Subject: [Histonet] Nuclear Bubbling In-Reply-To: <656599459.4038172.1455646347478.JavaMail.yahoo@mail.yahoo.com> References: <9B1A1501A800064397369BD8072E6BCA06503B35@E2K10DB.springfieldclinic.com> <656599459.4038172.1455646347478.JavaMail.yahoo@mail.yahoo.com> Message-ID: There is no need to be rude. He has tried the drying option and is still having nuclear bubbling. He is exploring other possible issues. You would see this if you read the email in its entirety. From: Rene J Buesa via Histonet To: "Vickroy, James" , "histonet at lists.utsouthwestern.edu" Date: 02/16/2016 10:13 AM Subject: Re: [Histonet] Nuclear Bubbling If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain ? "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.ren? On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" wrote: Struggling to find an answer. We do a lot of GI biopsies in our lab. Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense. Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times. I do not find that the problem is fixation. In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend). There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining. I really thought that this might be the issue however I'm not sure at this point. Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without. I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue. Here are my questions: 1. Could it be something that is happening with the tissue before it gets to the lab? Usually a delay if fixation causes other artifacts but not bubbling. Could it be heat from the GI procedure? 2. We do use blue sponges for our biopsies. I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3. What about the heat stage in our Prisma stainer? I am really getting frustrated. Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling". Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue. I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com< mailto:jvickroy at SpringfieldClinic.com> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From philip_manfre at merck.com Tue Feb 16 13:44:52 2016 From: philip_manfre at merck.com (Manfre, Philip) Date: Tue, 16 Feb 2016 14:44:52 -0500 Subject: [Histonet] Nuclear Bubbling In-Reply-To: <656599459.4038172.1455646347478.JavaMail.yahoo@mail.yahoo.com> References: <9B1A1501A800064397369BD8072E6BCA06503B35@E2K10DB.springfieldclinic.com> <656599459.4038172.1455646347478.JavaMail.yahoo@mail.yahoo.com> Message-ID: <558A4571351D0C42BD923F403F4198C40109FE02D4DF@USCTMXP51014.merck.com> Sort of a rude response to someone looking for help. -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, February 16, 2016 1:12 PM To: Vickroy, James; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain ? "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.ren? On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" wrote: Struggling to find an answer.? We do a lot of GI biopsies in our lab.? Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense.? Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times.? I do not find that the problem is fixation.? In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend).? There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining.? I really thought that this might be the issue however I'm not sure at this point.? Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without.? I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue.? Here are my questions: 1.? ? ? ? Could it be something that is happening with the tissue before it gets to the lab?? Usually a delay if fixation? causes other artifacts but not bubbling.? Could it be heat from the GI procedure? 2.? ? ? We do use blue sponges for our biopsies.? I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3.? ? ? What about the heat stage in our Prisma stainer? I am really getting frustrated.? Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling".? Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue.? I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois? 62703 Office:? 217-528-7541, Ext. 15121 Email:? jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From j.rowaihi at alborglaboratories.com Tue Feb 16 13:56:28 2016 From: j.rowaihi at alborglaboratories.com (Jamal Rowaihi) Date: Tue, 16 Feb 2016 22:56:28 +0300 Subject: [Histonet] Nuclear Bubbling Message-ID: Great, I agree? Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories?Sent from my cell phone-------- Original message --------From: "Manfre, Philip via Histonet" Date: 2/16/2016 10:44 PM (GMT+03:00) To: Rene J Buesa , "Vickroy, James" Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling Sort of a rude response to someone looking for help. -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, February 16, 2016 1:12 PM To: Vickroy, James; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain ? "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.ren? ??? On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" wrote: Struggling to find an answer.? We do a lot of GI biopsies in our lab.? Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense.? Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times.? I do not find that the problem is fixation.? In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend).? There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining.? I really thought that this might be the issue however I'm not sure at this point.? Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without.? I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue.? Here are my questions: 1.? ? ? ? Could it be something that is happening with the tissue before it gets to the lab?? Usually a delay if fixation? causes other artifacts but not bubbling.? Could it be heat from the GI procedure? 2.? ? ? We do use blue sponges for our biopsies.? I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3.? ? ? What about the heat stage in our Prisma stainer? I am really getting frustrated.? Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling".? Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue.? I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois? 62703 Office:? 217-528-7541, Ext. 15121 Email:? jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice:? This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jvickroy at SpringfieldClinic.com Tue Feb 16 15:53:00 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Tue, 16 Feb 2016 21:53:00 +0000 Subject: [Histonet] Nuclear Bubbling In-Reply-To: References: Message-ID: <9B1A1501A800064397369BD8072E6BCA06504834@E2K10DB.springfieldclinic.com> For the record please note that I have over thirty-six years experience working in a Histology lab. I have been a supervisor or manager of a hospital and clinic histology department for at least 25 years. Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com From: Jamal Rowaihi [mailto:j.rowaihi at alborglaboratories.com] Sent: Tuesday, February 16, 2016 1:56 PM To: Manfre, Philip; Rene J Buesa; Vickroy, James Cc: ???? ???????; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling Great, I agree Regards Jamal Rowaihi Anatomic Pathology Supervisor Al Borg Medical Laboratories Sent from my cell phone -------- Original message -------- From: "Manfre, Philip via Histonet" > Date: 2/16/2016 10:44 PM (GMT+03:00) To: Rene J Buesa >, "Vickroy, James" > Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling Sort of a rude response to someone looking for help. -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, February 16, 2016 1:12 PM To: Vickroy, James; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain ? "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.ren? On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" > wrote: Struggling to find an answer. We do a lot of GI biopsies in our lab. Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense. Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times. I do not find that the problem is fixation. In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend). There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining. I really thought that this might be the issue however I'm not sure at this point. Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without. I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue. Here are my questions: 1. Could it be something that is happening with the tissue before it gets to the lab? Usually a delay if fixation causes other artifacts but not bubbling. Could it be heat from the GI procedure? 2. We do use blue sponges for our biopsies. I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3. What about the heat stage in our Prisma stainer? I am really getting frustrated. Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling". Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue. I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From latecor at adinet.com.uy Tue Feb 16 15:56:49 2016 From: latecor at adinet.com.uy (Carlos Defeo) Date: Tue, 16 Feb 2016 21:56:49 +0000 Subject: [Histonet] nuclear bubbling Message-ID: Dear James: Two chained events take place on your issue: 1-sections not entirely drained,water remains even minimally under sections; 2- you put these sections in the oven, the heat literally "explodes" the nuclear bubbles and creates a hole with no cromatin to stain. My kind regards, Carlos Defeo Histotechnologist From derm.katiesands at gmail.com Tue Feb 16 16:18:03 2016 From: derm.katiesands at gmail.com (Katie Sands) Date: Tue, 16 Feb 2016 16:18:03 -0600 Subject: [Histonet] Nuclear Bubbling In-Reply-To: <9B1A1501A800064397369BD8072E6BCA06504834@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA06504834@E2K10DB.springfieldclinic.com> Message-ID: I don't have your answer Jim, yet I can vouch for your experience as a supervisor since you were my boss for about two years. Hopefully you can get it resolved because it can be very frustrating as the tech. On Tuesday, February 16, 2016, Vickroy, James via Histonet < histonet at lists.utsouthwestern.edu> wrote: > For the record please note that I have over thirty-six years experience > working in a Histology lab. I have been a supervisor or manager of a > hospital and clinic histology department for at least 25 years. > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvickroy at SpringfieldClinic.com jvickroy at SpringfieldClinic.com > > > > From: Jamal Rowaihi [mailto:j.rowaihi at alborglaboratories.com > ] > Sent: Tuesday, February 16, 2016 1:56 PM > To: Manfre, Philip; Rene J Buesa; Vickroy, James > Cc: ???? ???????; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Nuclear Bubbling > > Great, I agree > > > > Regards > > Jamal Rowaihi > Anatomic Pathology Supervisor > Al Borg Medical Laboratories > Sent from my cell phone > -------- Original message -------- > From: "Manfre, Philip via Histonet" >> > Date: 2/16/2016 10:44 PM (GMT+03:00) > To: Rene J Buesa rjbuesa at yahoo.com >>, "Vickroy, James" > >> > Cc: histonet at lists.utsouthwestern.edu histonet at lists.utsouthwestern.edu > > Subject: Re: [Histonet] Nuclear Bubbling > > Sort of a rude response to someone looking for help. > > -----Original Message----- > From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu > ] > Sent: Tuesday, February 16, 2016 1:12 PM > To: Vickroy, James; histonet at lists.utsouthwestern.edu > > > Subject: Re: [Histonet] Nuclear Bubbling > > If I remember correctly, this issue has been discussed previously.The > general consensus as to the cause of nuclear "bubbling" (in reality a lack > of staining in the nuclear area) has been attributed to an incomplete > section drying.After the section has be "fished" from the water bath, if > the slide is not set to drain the underneath water before drying, the > nuclear components are dissolved hence when the section is stained, there > is nothing to stain ? "nuclear bubbling".I think this has been previously > stated so I really do not understand posting this same question again.I do > not think that posting again the question a different answer is going to be > received.ren? > > On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" < > histonet at lists.utsouthwestern.edu histonet at lists.utsouthwestern.edu >> wrote: > > > > Struggling to find an answer. We do a lot of GI biopsies in our lab. > Sometimes they look wonderful without any nuclear bubbling, other times the > bubbling is pretty intense. Since nuclear bubbling is often attributed to > incomplete fixation we of course have investigated the fixation times. I > do not find that the problem is fixation. In fact some of the biopsies end > up fixing for 48 hrs before processing. (weekend). There was a suggestion > last week or so that there might be water trapped under the slides after > cutting and before staining. I really thought that this might be the issue > however I'm not sure at this point. Extra drying seems to help but > sometimes slides side by side are so variable, one with bubbles and one > without. I also don't believe the problem is in the processing schedule > since the problem has shown up on both a rapid and a normal schedule. > (therefore longer dehydration, clearing, etc.) > > I am wondering if anyone else has worked with this issue. Here are my > questions: > > > 1. Could it be something that is happening with the tissue before > it gets to the lab? Usually a delay if fixation causes other artifacts > but not bubbling. Could it be heat from the GI procedure? > > 2. We do use blue sponges for our biopsies. I know some say get rid > of the sponges but has anyone seen this problem caused by usage of sponges? > > 3. What about the heat stage in our Prisma stainer? > > > I am really getting frustrated. Pathologists never complain however I > would rather all of the tissue did not have the "nuclear bubbling". Again > we only do biopsies so I really don't think the standard old " not enough > time in formalin" is the issue. I have even wondered about variables such > as we use recycled formalin, recycled Clearite III. > > Any suggestions? > > Jim > > > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvickroy at SpringfieldClinic.com jvickroy at SpringfieldClinic.com jvickroy at SpringfieldClinic.com > %3cmailto:jvickroy at SpringfieldClinic.com>> > > > > This electronic message contains information from Springfield Clinic, LLP > that may be confidential, privileged, and/or sensitive. This information is > intended for the use of the individual(s) or entity(ies) named above. If > you are not the intended recipient, be aware that disclosure, copying, > distribution, or action taken on the contents of this information is > strictly prohibited. If you have received this electronic message in error, > please notify the sender immediately, by electronic mail, so that > arrangements may be made for the retrieval of this electronic message. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, > New Jersey, USA 07033), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Katie L. Sands Histology Technician/Lab Manager Advanced Dermatology of Southeast Missouri, PC 2116 Megan Drive Suite 102 Cape Girardeau, MO 63701 derm.katiesands at gmail.com Work: (573) 335-7546 Cell: (309) 840-3799 www.dermsemo.com From cgenty at criticalxsolutions.com Wed Feb 17 10:31:51 2016 From: cgenty at criticalxsolutions.com (Carlos Genty) Date: Wed, 17 Feb 2016 16:31:51 +0000 Subject: [Histonet] Sequenza Immunostaining Racks Message-ID: Good Day Everyone, I am looking to obtain used Thermo Sequenza Immunostaining Racks (about 10 or so). Does anyone have any that they would be willing to part with? Please feel free to contact me directly. Thanks in advance! Carlos Carlos Genty Critical X Solutions, LLC cgenty at criticalxsolutions.com (310) 357-1993 From craigak12 at gmail.com Wed Feb 17 11:03:45 2016 From: craigak12 at gmail.com (Jb) Date: Wed, 17 Feb 2016 10:03:45 -0700 Subject: [Histonet] Error Prevention: Message-ID: <9ED89D91-CB8E-4014-B24A-6C91314414B8@gmail.com> Does anyone have a policy that they are willing to share regarding tech errors, error prevention, etc? How do you document, retrain, write up, and terminate employees for errors? What is considered severe problems for termination, etc. I know it is up to the manager, I want to know what other lab standards are to help create a procedure. Thank you, Sent from my iPhone From relia1 at earthlink.net Wed Feb 17 11:12:03 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 17 Feb 2016 12:12:03 -0500 Subject: [Histonet] RELIA HOT Histology Jobs Alert! 2-17-2016 Happy Hump Day! Message-ID: <003001d169a6$524fbca0$f6ef35e0$@earthlink.net> Hello Histopeeps! Happy Hump Day! We are halfway to the weekend! We GOT This!! Since it's mid-week and my phone is ringing off the hook with new opportunities I wanted to do a quick post to tell you about the positions that I am working on and am MOST excited about. Why am I excited about these positions? Because these clients offer excellent compensation, benefits, relocation assistance leading edge training! But most importantly they are ready to interview and hire ASAP. All of these positions are full time and permanent. These are the histology positions that i am most excited about! LEADERSHIP/SUPERVISORY: Lab Manager - Williamsburg, VA Histology Supervisor - Texas Lead Clinical Specialist - Pathology - Virginia (15K sign on) Lead Histotechnologist - Run your own lab - Fayetteville, AR Lead Histotechnologist - Run your own lab - Great Falls, MT TECHNICIANS/TECHNOLOGISTS: Norfolk, VA - 15K sign on bonus!! Kansas City, KS - days dermpath learn Mohs Los Angeles, CA - IHC Specialist Louisville, KY - Learn Electron Microscopy Fayetteville, AR - Run your own dermpath lab Great Falls, MT - Run your own path lab If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I place someone you refer, You will earn a referral fee. I can be reached toll free at the office at 866-607-3542 or relia1 at earthlink.net or you can always catch me on cell call or text me at 407-353-5070. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Jose.R.deGuzman at gunet.georgetown.edu Wed Feb 17 11:55:41 2016 From: Jose.R.deGuzman at gunet.georgetown.edu (deGuzman, Jose R) Date: Wed, 17 Feb 2016 12:55:41 -0500 Subject: [Histonet] Nuclear Bubbling In-Reply-To: References: Message-ID: Jim, Are you drying the slides in an oven before the heat stage on the Prisma? If you use an oven, what is the temperature setting and for how long are slides kept in? Carson and Hladik recommends 60 degrees for less than 1 hour. You mentioned, recycled formalin, what is the pH of the formalin? I apologize for all the questions and no answers. Jose MedStar Health is a not-for-profit, integrated healthcare delivery system, the largest in Maryland and the Washington, D.C., region. Nationally recognized for clinical quality in heart, orthopaedics, cancer and GI. IMPORTANT: This e-mail (including any attachments) may contain information that is private, confidential, or protected by attorney-client or other privilege. If you received this e-mail in error, please delete it from your system without copying it and notify sender by reply e-mail, so that our records can be corrected. Thank you. Help conserve valuable resources - only print this email if necessary. From Aubrey at nsh.org Wed Feb 17 12:00:27 2016 From: Aubrey at nsh.org (Aubrey Wanner) Date: Wed, 17 Feb 2016 18:00:27 +0000 Subject: [Histonet] Nuclear Bubbling Message-ID: NSH has an archived webinar: Nuclear Bubbling: It's About Time" created by Peggy Wenk. The webinar talks about several causes of nuclear bubbling, changes that can be made to reduce this problem, and, for really bad cases, Peggy talks about how to dry the slide differently, reprocess tissue or use a HIER technique to improve the quality of the nuclear detail. It's available to members through our online community: The Block: theblock.nsh.org Aubrey M.J. Wanner National Society for Histotechnology -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Wednesday, February 17, 2016 12:04 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 147, Issue 16 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Sakura Pre-Processing Fixative (7117) for X120 Processors (Jonathan Jennings) 2. Re: Nuclear Bubbling (Rene J Buesa) 3. (CLRW) Clinical Laboratory Reagent Water (Brent Adams) 4. Re: Nuclear Bubbling (Jennifer MacDonald) 5. Re: Nuclear Bubbling (Manfre, Philip) 6. Re: Nuclear Bubbling (Jamal Rowaihi) 7. Re: Nuclear Bubbling (Vickroy, James) 8. nuclear bubbling (Carlos Defeo) 9. Re: Nuclear Bubbling (Katie Sands) 10. Sequenza Immunostaining Racks (Carlos Genty) 11. Error Prevention: (Jb) ---------------------------------------------------------------------- Message: 1 Date: Tue, 16 Feb 2016 18:00:59 +0000 From: Jonathan Jennings To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Sakura Pre-Processing Fixative (7117) for X120 Processors Message-ID: <248395418C8C074A83C7D06081C4F01634741ADB at DERMLAB-SBS.dermlab.local> Content-Type: text/plain; charset="us-ascii" Hello All, I manage a histology Dermpath lab (We only process skins) and we use Sakura X120 Processors. We have been doing excellent with the use of our x120 processors that allowed use to have slides on the Pathologist desk within 5 hours from the time we received the specimens. That said, there is always room for improvement! As anyone who uses the Sakura x120 Xpress processors know, all of the solutions and reagents required are "proprietary" so I don't know exactly what is in them. To top that off, we bought the processors from a 3rd party vendor, so Sakura isn't too quick to come out and help me with this. All of That noted, the reason I am sending this email is because I want to incorporate the pre-processing fixative because the specimens we receive in the afternoon haven't been in formalin as long (3-5 hours) as the specimens that we receive by mail in the morning (in formalin for 18+ hours). Our preprocessing solution is working ok, but I read in the user manual about preprocessing fixative that should be used instead, if the specimens were not completely fixed before processing. Here are a few details of our current processing workflow with only using preprocessing solution. * When grossing we have 3 small stirring hot plates set at 38 Celsius. Each stir plate described below o 1 for the 0.2 or < Biopsies filled with 10% NBF prior to preprocessing solution for 15 minutes then processed on standard (1 hour program) o 1 for the 0.3 or > Biopsies Filled with Statfix (statlab) Must be in statfix. Cassettes must be in for at least 30 minutes prior to preprocessing solution (30 minutes) Then Placed on the processor (Extended - 2 hour program) o 1 for the excisions, Cyst and all other fatty skin specimens, filled with statfix Cassettes must be in for at least 1 hour prior to preprocessing solution (30 minutes) The placed on processor (Extended - 2 hour program) I tried the preprocessing fixative (no heat, just stirring agitation) on test excisions and it definitely made the tissue more firm, (almost too hard) for 30 minutes. We had to use preprocessing solution for 1 hour on excisions, so that is a 30 minute improvement. It also turned the tissue yellow, which was weird. Before I try to test more tissue, I wanted to see if anyone else has went through the same preprocessing fixative testing with skins. Questions Finally! ;) * Does anyone use the preprocessing fixative for skin specimens and would you mind giving me some insight/advice on how to incorporate? * Is it ok to incorporate any heat to the preprocessing solution or preprocessing fixative to speed up processing time? * Does the preprocessing fixative affect IHCs any differently than the preprocessing solution? * Does anyone have any comments/ideas about my inquiry? I apologize for the lengthy post. Thank you for taking the time to read it. I hope everyone has a blessed day! This e-mail transmission, and any documents, files or previous e-mail messages attached to it, may contain confidential information. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of any of the information contained in or attached to this message is strictly prohibited. If you have received this transmission in error, please immediately notify Jonathan Jennings by reply email or by telephone 1-855-705-1776, and destroy the original transmission and its attachments without reading them or saving them to any media storage device. ------------------------------ Message: 2 Date: Tue, 16 Feb 2016 18:12:27 +0000 (UTC) From: Rene J Buesa To: "Vickroy, James" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Nuclear Bubbling Message-ID: <656599459.4038172.1455646347478.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain ? "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.ren? On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" wrote: Struggling to find an answer.? We do a lot of GI biopsies in our lab.? Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense.? Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times.? I do not find that the problem is fixation.? In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend).? There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining.? I really thought that this might be the issue however I'm not sure at this point.? Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without.? I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue.? Here are my questions: 1.? ? ? ? Could it be something that is happening with the tissue before it gets to the lab?? Usually a delay if fixation? causes other artifacts but not bubbling.? Could it be heat from the GI procedure? 2.? ? ? We do use blue sponges for our biopsies.? I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3.? ? ? What about the heat stage in our Prisma stainer? I am really getting frustrated.? Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling".? Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue.? I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois? 62703 Office:? 217-528-7541, Ext. 15121 Email:? jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 16 Feb 2016 18:34:46 +0000 From: Brent Adams To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] (CLRW) Clinical Laboratory Reagent Water Message-ID: Content-Type: text/plain; charset="Windows-1252" Deanne, I purchase 5 liter cubes from Mercedes Medical. They can provide test results on each batch of water and fax to you. I did have a CLIA inspector advise to document daily on the water as is written on the cube for clarity of water, no sediments or signs of contamination. Think 5 Liters is about $12.50 and I use a little less than two a month. Brent Adams ? BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, February 16, 2016 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 147, Issue 15 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. (CLRW) - Clinical Laboratory Reagent Water (Knutson, Deanne) 2. Tissue cassette baskets for VIP 6 (Vickroy, James) 3. MICROTOME KNIFE SHARPENING (Hannen, Valerie) 4. Re: (CLRW) - Clinical Laboratory Reagent Water (Morken, Timothy) 5. Nuclear Bubbling (Vickroy, James) 6. Complementary webinar on post-processing scientific images, Feb 24 @ 1PM EST (J. Sedgewick) ---------------------------------------------------------------------- Message: 1 Date: Mon, 15 Feb 2016 12:14:06 -0600 From: "Knutson, Deanne" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] (CLRW) - Clinical Laboratory Reagent Water Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A4D5970FFC at EXCHANGE2K7.staprimecare.org> Content-Type: text/plain; charset="us-ascii" Fellow Histonetters, Our medical center will no longer be providing us with water for our laboratory procedures. I was wondering where other labs who need to purchase Clinical Laboratory Reagent Water are doing so? Thank you for your help! Deanne Knutson Supervisor Anatomic Pathology ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ------------------------------ Message: 2 Date: Mon, 15 Feb 2016 19:12:06 +0000 From: "Vickroy, James" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Tissue cassette baskets for VIP 6 Message-ID: <9B1A1501A800064397369BD8072E6BCA06502BD8 at E2K10DB.springfieldclinic.com> Content-Type: text/plain; charset="us-ascii" Anybody have an idea where we can get a used cassette basket that will fit in the VIP 6? The cost of a new one is pretty high? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ------------------------------ Message: 3 Date: Tue, 16 Feb 2016 10:28:59 -0500 From: "Hannen, Valerie" To: "Histonet at lists.utsouthwestern.edu" Subject: [Histonet] MICROTOME KNIFE SHARPENING Message-ID: <450B7A81EDA0C54E97C53D60F00776C323546750BC at isexstore03> Content-Type: text/plain; charset="us-ascii" Hi all... Hoping you might be answer a couple of questions that I have. 1) Does anyone use a microtome knife sharpening kit made by Pathco? If so, 2) How well does it sharpen the blades? 3) Is there any issue on attaching the abrasive sheets to the honing plate? 4) Do the abrasive sheets tend to "move" during the sharpening process, that would cause the blade not be on them but on the plate instead? I currently am using coarse and fine abrasive liquids along with the honing plate... but the coarse, fine and honing liquids are becoming quite expensive and hard to come by. Any and all replies are welcomed. Thank you in advance, Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== ------------------------------ Message: 4 Date: Tue, 16 Feb 2016 16:05:12 +0000 From: "Morken, Timothy" To: "Knutson, Deanne" Cc: Histonet Subject: Re: [Histonet] (CLRW) - Clinical Laboratory Reagent Water Message-ID: <761E2B5697F795489C8710BCC72141FF6FD17480 at ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Deanne, If you just need it for a few critical reagents You can get type 1 water by the pint or the gallon from Fisher (NERL Type 1 water). It's probably overkill for most histology procedures, but convenient if you need it. Note that the expiry clock of 30 days starts when you open the bottle. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Knutson, Deanne via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, February 15, 2016 10:14 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] (CLRW) - Clinical Laboratory Reagent Water Fellow Histonetters, Our medical center will no longer be providing us with water for our laboratory procedures. I was wondering where other labs who need to purchase Clinical Laboratory Reagent Water are doing so? Thank you for your help! Deanne Knutson Supervisor Anatomic Pathology ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 16 Feb 2016 17:10:40 +0000 From: "Vickroy, James" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Nuclear Bubbling Message-ID: <9B1A1501A800064397369BD8072E6BCA06503B35 at E2K10DB.springfieldclinic.com> Content-Type: text/plain; charset="us-ascii" Struggling to find an answer. We do a lot of GI biopsies in our lab. Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense. Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times. I do not find that the problem is fixation. In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend). There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining. I really thought that this might be the issue however I'm not sure at this point. Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without. I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue. Here are my questions: 1. Could it be something that is happening with the tissue before it gets to the lab? Usually a delay if fixation causes other artifacts but not bubbling. Could it be heat from the GI procedure? 2. We do use blue sponges for our biopsies. I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3. What about the heat stage in our Prisma stainer? I am really getting frustrated. Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling". Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue. I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ------------------------------ Message: 6 Date: Tue, 16 Feb 2016 11:31:27 -0600 From: "J. Sedgewick" To: Subject: [Histonet] Complementary webinar on post-processing scientific images, Feb 24 @ 1PM EST Message-ID: <1557B579CB2A478B884FD20B9E5064D6 at sedge> Content-Type: text/plain; format=flowed; charset="UTF-8"; reply-type=original Hello All, Do you post-process your scientific images? Typical tools for common adjustments include Photoshop and ImageJ, but they aren?t the best answer. You are invited to attend a complementary webinar on Feb 24 at 1:00PM EST ? ?Best Practices for Post-Processing of Scientific Images? Reserve your webinar seat now at https://attendee.gotowebinar.com/register/1379379490730827010 I'll be giving this webinar. Also, a similar presentation will be given for the histology WOW 2016 with Dr. Michael Linden at the University of Minnesota on March 25. Best, Jerry Sedgewick ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 147, Issue 15 ***************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. ------------------------------ Message: 4 Date: Tue, 16 Feb 2016 11:33:47 -0800 From: Jennifer MacDonald To: Rene J Buesa Cc: "histonet at lists.utsouthwestern.edu" , "Vickroy, James" Subject: Re: [Histonet] Nuclear Bubbling Message-ID: Content-Type: text/plain; charset="GB2312" There is no need to be rude. He has tried the drying option and is still having nuclear bubbling. He is exploring other possible issues. You would see this if you read the email in its entirety. From: Rene J Buesa via Histonet To: "Vickroy, James" , "histonet at lists.utsouthwestern.edu" Date: 02/16/2016 10:13 AM Subject: Re: [Histonet] Nuclear Bubbling If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain ? "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.ren? On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" wrote: Struggling to find an answer. We do a lot of GI biopsies in our lab. Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense. Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times. I do not find that the problem is fixation. In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend). There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining. I really thought that this might be the issue however I'm not sure at this point. Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without. I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue. Here are my questions: 1. Could it be something that is happening with the tissue before it gets to the lab? Usually a delay if fixation causes other artifacts but not bubbling. Could it be heat from the GI procedure? 2. We do use blue sponges for our biopsies. I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3. What about the heat stage in our Prisma stainer? I am really getting frustrated. Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling". Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue. I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com< mailto:jvickroy at SpringfieldClinic.com> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 16 Feb 2016 14:44:52 -0500 From: "Manfre, Philip" To: Rene J Buesa , "Vickroy, James" Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Nuclear Bubbling Message-ID: <558A4571351D0C42BD923F403F4198C40109FE02D4DF at USCTMXP51014.merck.com> Content-Type: text/plain; charset="utf-8" Sort of a rude response to someone looking for help. -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, February 16, 2016 1:12 PM To: Vickroy, James; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain ? "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.ren? On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" wrote: Struggling to find an answer.? We do a lot of GI biopsies in our lab.? Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense.? Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times.? I do not find that the problem is fixation.? In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend).? There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining.? I really thought that this might be the issue however I'm not sure at this point.? Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without.? I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue.? Here are my questions: 1.? ? ? ? Could it be something that is happening with the tissue before it gets to the lab?? Usually a delay if fixation? causes other artifacts but not bubbling.? Could it be heat from the GI procedure? 2.? ? ? We do use blue sponges for our biopsies.? I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3.? ? ? What about the heat stage in our Prisma stainer? I am really getting frustrated.? Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling".? Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue.? I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois? 62703 Office:? 217-528-7541, Ext. 15121 Email:? jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 6 Date: Tue, 16 Feb 2016 22:56:28 +0300 From: Jamal Rowaihi To: "Manfre, Philip" , Rene J Buesa , "Vickroy, James" Cc: ???? ??????? , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Nuclear Bubbling Message-ID: Content-Type: text/plain; charset=utf-8 Great, I agree? Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories?Sent from my cell phone-------- Original message --------From: "Manfre, Philip via Histonet" Date: 2/16/2016 10:44 PM (GMT+03:00) To: Rene J Buesa , "Vickroy, James" Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling Sort of a rude response to someone looking for help. -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, February 16, 2016 1:12 PM To: Vickroy, James; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain ? "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.ren? ??? On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" wrote: Struggling to find an answer.? We do a lot of GI biopsies in our lab.? Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense.? Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times.? I do not find that the problem is fixation.? In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend).? There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining.? I really thought that this might be the issue however I'm not sure at this point.? Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without.? I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue.? Here are my questions: 1.? ? ? ? Could it be something that is happening with the tissue before it gets to the lab?? Usually a delay if fixation? causes other artifacts but not bubbling.? Could it be heat from the GI procedure? 2.? ? ? We do use blue sponges for our biopsies.? I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3.? ? ? What about the heat stage in our Prisma stainer? I am really getting frustrated.? Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling".? Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue.? I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois? 62703 Office:? 217-528-7541, Ext. 15121 Email:? jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice:? This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 16 Feb 2016 21:53:00 +0000 From: "Vickroy, James" To: 'Jamal Rowaihi' , "Manfre, Philip" , Rene J Buesa Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Nuclear Bubbling Message-ID: <9B1A1501A800064397369BD8072E6BCA06504834 at E2K10DB.springfieldclinic.com> Content-Type: text/plain; charset="utf-8" For the record please note that I have over thirty-six years experience working in a Histology lab. I have been a supervisor or manager of a hospital and clinic histology department for at least 25 years. Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com From: Jamal Rowaihi [mailto:j.rowaihi at alborglaboratories.com] Sent: Tuesday, February 16, 2016 1:56 PM To: Manfre, Philip; Rene J Buesa; Vickroy, James Cc: ???? ???????; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling Great, I agree Regards Jamal Rowaihi Anatomic Pathology Supervisor Al Borg Medical Laboratories Sent from my cell phone -------- Original message -------- From: "Manfre, Philip via Histonet" > Date: 2/16/2016 10:44 PM (GMT+03:00) To: Rene J Buesa >, "Vickroy, James" > Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling Sort of a rude response to someone looking for help. -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, February 16, 2016 1:12 PM To: Vickroy, James; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling If I remember correctly, this issue has been discussed previously.The general consensus as to the cause of nuclear "bubbling" (in reality a lack of staining in the nuclear area) has been attributed to an incomplete section drying.After the section has be "fished" from the water bath, if the slide is not set to drain the underneath water before drying, the nuclear components are dissolved hence when the section is stained, there is nothing to stain ? "nuclear bubbling".I think this has been previously stated so I really do not understand posting this same question again.I do not think that posting again the question a different answer is going to be received.ren? On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" > wrote: Struggling to find an answer. We do a lot of GI biopsies in our lab. Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense. Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times. I do not find that the problem is fixation. In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend). There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining. I really thought that this might be the issue however I'm not sure at this point. Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without. I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue. Here are my questions: 1. Could it be something that is happening with the tissue before it gets to the lab? Usually a delay if fixation causes other artifacts but not bubbling. Could it be heat from the GI procedure? 2. We do use blue sponges for our biopsies. I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3. What about the heat stage in our Prisma stainer? I am really getting frustrated. Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling". Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue. I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 16 Feb 2016 21:56:49 +0000 From: "Carlos Defeo" To: "histonet at lists.utsouthwestern.edu" Cc: histonet at lists.utsouthwestern.edu Subject: [Histonet] nuclear bubbling Message-ID: Content-Type: text/plain; format=flowed; charset=utf-8 Dear James: Two chained events take place on your issue: 1-sections not entirely drained,water remains even minimally under sections; 2- you put these sections in the oven, the heat literally "explodes" the nuclear bubbles and creates a hole with no cromatin to stain. My kind regards, Carlos Defeo Histotechnologist ------------------------------ Message: 9 Date: Tue, 16 Feb 2016 16:18:03 -0600 From: Katie Sands To: "Vickroy, James" Cc: Jamal Rowaihi , "Manfre, Philip" , Rene J Buesa , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Nuclear Bubbling Message-ID: Content-Type: text/plain; charset=UTF-8 I don't have your answer Jim, yet I can vouch for your experience as a supervisor since you were my boss for about two years. Hopefully you can get it resolved because it can be very frustrating as the tech. On Tuesday, February 16, 2016, Vickroy, James via Histonet < histonet at lists.utsouthwestern.edu> wrote: > For the record please note that I have over thirty-six years experience > working in a Histology lab. I have been a supervisor or manager of a > hospital and clinic histology department for at least 25 years. > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvickroy at SpringfieldClinic.com jvickroy at SpringfieldClinic.com > > > > From: Jamal Rowaihi [mailto:j.rowaihi at alborglaboratories.com > ] > Sent: Tuesday, February 16, 2016 1:56 PM > To: Manfre, Philip; Rene J Buesa; Vickroy, James > Cc: ???? ???????; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Nuclear Bubbling > > Great, I agree > > > > Regards > > Jamal Rowaihi > Anatomic Pathology Supervisor > Al Borg Medical Laboratories > Sent from my cell phone > -------- Original message -------- > From: "Manfre, Philip via Histonet" >> > Date: 2/16/2016 10:44 PM (GMT+03:00) > To: Rene J Buesa rjbuesa at yahoo.com >>, "Vickroy, James" > >> > Cc: histonet at lists.utsouthwestern.edu histonet at lists.utsouthwestern.edu > > Subject: Re: [Histonet] Nuclear Bubbling > > Sort of a rude response to someone looking for help. > > -----Original Message----- > From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu > ] > Sent: Tuesday, February 16, 2016 1:12 PM > To: Vickroy, James; histonet at lists.utsouthwestern.edu > > > Subject: Re: [Histonet] Nuclear Bubbling > > If I remember correctly, this issue has been discussed previously.The > general consensus as to the cause of nuclear "bubbling" (in reality a lack > of staining in the nuclear area) has been attributed to an incomplete > section drying.After the section has be "fished" from the water bath, if > the slide is not set to drain the underneath water before drying, the > nuclear components are dissolved hence when the section is stained, there > is nothing to stain ? "nuclear bubbling".I think this has been previously > stated so I really do not understand posting this same question again.I do > not think that posting again the question a different answer is going to be > received.ren? > > On Tuesday, February 16, 2016 12:32 PM, "Vickroy, James via Histonet" < > histonet at lists.utsouthwestern.edu histonet at lists.utsouthwestern.edu >> wrote: > > > > Struggling to find an answer. We do a lot of GI biopsies in our lab. > Sometimes they look wonderful without any nuclear bubbling, other times the > bubbling is pretty intense. Since nuclear bubbling is often attributed to > incomplete fixation we of course have investigated the fixation times. I > do not find that the problem is fixation. In fact some of the biopsies end > up fixing for 48 hrs before processing. (weekend). There was a suggestion > last week or so that there might be water trapped under the slides after > cutting and before staining. I really thought that this might be the issue > however I'm not sure at this point. Extra drying seems to help but > sometimes slides side by side are so variable, one with bubbles and one > without. I also don't believe the problem is in the processing schedule > since the problem has shown up on both a rapid and a normal schedule. > (therefore longer dehydration, clearing, etc.) > > I am wondering if anyone else has worked with this issue. Here are my > questions: > > > 1. Could it be something that is happening with the tissue before > it gets to the lab? Usually a delay if fixation causes other artifacts > but not bubbling. Could it be heat from the GI procedure? > > 2. We do use blue sponges for our biopsies. I know some say get rid > of the sponges but has anyone seen this problem caused by usage of sponges? > > 3. What about the heat stage in our Prisma stainer? > > > I am really getting frustrated. Pathologists never complain however I > would rather all of the tissue did not have the "nuclear bubbling". Again > we only do biopsies so I really don't think the standard old " not enough > time in formalin" is the issue. I have even wondered about variables such > as we use recycled formalin, recycled Clearite III. > > Any suggestions? > > Jim > > > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvickroy at SpringfieldClinic.com jvickroy at SpringfieldClinic.com jvickroy at SpringfieldClinic.com > %3cmailto:jvickroy at SpringfieldClinic.com>> > > > > This electronic message contains information from Springfield Clinic, LLP > that may be confidential, privileged, and/or sensitive. This information is > intended for the use of the individual(s) or entity(ies) named above. If > you are not the intended recipient, be aware that disclosure, copying, > distribution, or action taken on the contents of this information is > strictly prohibited. If you have received this electronic message in error, > please notify the sender immediately, by electronic mail, so that > arrangements may be made for the retrieval of this electronic message. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, > New Jersey, USA 07033), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Katie L. Sands Histology Technician/Lab Manager Advanced Dermatology of Southeast Missouri, PC 2116 Megan Drive Suite 102 Cape Girardeau, MO 63701 derm.katiesands at gmail.com Work: (573) 335-7546 Cell: (309) 840-3799 www.dermsemo.com ------------------------------ Message: 10 Date: Wed, 17 Feb 2016 16:31:51 +0000 From: Carlos Genty To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Sequenza Immunostaining Racks Message-ID: Content-Type: text/plain; charset="iso-8859-1" Good Day Everyone, I am looking to obtain used Thermo Sequenza Immunostaining Racks (about 10 or so). Does anyone have any that they would be willing to part with? Please feel free to contact me directly. Thanks in advance! Carlos Carlos Genty Critical X Solutions, LLC cgenty at criticalxsolutions.com (310) 357-1993 ------------------------------ Message: 11 Date: Wed, 17 Feb 2016 10:03:45 -0700 From: Jb To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Error Prevention: Message-ID: <9ED89D91-CB8E-4014-B24A-6C91314414B8 at gmail.com> Content-Type: text/plain; charset=us-ascii Does anyone have a policy that they are willing to share regarding tech errors, error prevention, etc? How do you document, retrain, write up, and terminate employees for errors? What is considered severe problems for termination, etc. I know it is up to the manager, I want to know what other lab standards are to help create a procedure. Thank you, Sent from my iPhone ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 147, Issue 16 ***************************************** From tabbott at pennstatehealth.psu.edu Wed Feb 17 12:30:45 2016 From: tabbott at pennstatehealth.psu.edu (Abbott, Tanya) Date: Wed, 17 Feb 2016 18:30:45 +0000 Subject: [Histonet] Bar Code and Tracking Message-ID: Hello, I manage a small Pathology lab where we generate approx. 80-140 blocks for Histo and 5-6 Cytology fluids a day. I have 2 Histotechs that swap doing grossing monthly. I have 1 full time and 1 part time Cytotech and a Cyto assistant. I am curious if there are any smaller labs out there that have gone to a Bar Code and Tracking system and can offer any input. I came from a much larger lab that used CoPath from accessioning to sign-out, so that is the kind of system I am used to. It is certainly a system I wish to integrate in to my lab on a much smaller level, we currently have a very manual process, other than some entry/labeling in our LIS. Any input is appreciated! Tanya Tanya G. Abbott Pathology Manager Penn State Health St. Joseph Pathology Department (o) 610-378-2635 (f) 670-898-5871 tabbott at pennstatehealth.psu.edu Penn State Health St. Joseph | The Future of Healthcare From elaineahoffman55 at yahoo.com Wed Feb 17 14:17:43 2016 From: elaineahoffman55 at yahoo.com (Elaine allison Hoffman) Date: Wed, 17 Feb 2016 20:17:43 +0000 (UTC) Subject: [Histonet] New employees References: <2110457214.4608911.1455740263652.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <2110457214.4608911.1455740263652.JavaMail.yahoo@mail.yahoo.com> Greetings all, Does anyone have a new employee check-off worksheet that they would be willing to share for histotech's?Thanks in advance! Elaine Hoffman From Kristopher.Kalleberg at unilever.com Wed Feb 17 14:36:34 2016 From: Kristopher.Kalleberg at unilever.com (Kalleberg, Kristopher) Date: Wed, 17 Feb 2016 20:36:34 +0000 Subject: [Histonet] keratohyalin granules Message-ID: Hello All, Does anyone have experience staining the keratohyalin granules in skin with different types of hematoxylins? I am currently looking at keratohyalin granules and staining with Harris hematoxylin and there seems to be little staining and not as prominent as I would have expected. Wonder if anyone has noticed that different hematoxlins stain keratohyalin granules better than others. The skin is normal skin photoprotected and photoexposed. Thank you in advance for any helpful insights. Kris From W.E.J.Hoekert at olvg.nl Thu Feb 18 01:54:19 2016 From: W.E.J.Hoekert at olvg.nl (Hoekert, Willem) Date: Thu, 18 Feb 2016 07:54:19 +0000 Subject: [Histonet] Nuclear Bubbling In-Reply-To: <9B1A1501A800064397369BD8072E6BCA06503B35@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA06503B35@E2K10DB.springfieldclinic.com> Message-ID: Could it be due to incomplete deparaffinization? Willem ________________________________________ Van: Vickroy, James via Histonet [histonet at lists.utsouthwestern.edu] Verzonden: dinsdag 16 februari 2016 18:10 Aan: histonet at lists.utsouthwestern.edu Onderwerp: [Histonet] Nuclear Bubbling Struggling to find an answer. We do a lot of GI biopsies in our lab. Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense. Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times. I do not find that the problem is fixation. In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend). There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining. I really thought that this might be the issue however I'm not sure at this point. Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without. I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue. Here are my questions: 1. Could it be something that is happening with the tissue before it gets to the lab? Usually a delay if fixation causes other artifacts but not bubbling. Could it be heat from the GI procedure? 2. We do use blue sponges for our biopsies. I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3. What about the heat stage in our Prisma stainer? I am really getting frustrated. Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling". Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue. I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met elektronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. De algemene Inkoopvoorwaarden van de Vereniging Santeon respectievelijk de leden van de Vereniging Santeon zijn van toepassing op en maken integraal onderdeel uit van alle rechtsbetrekkingen, daaronder mede verstaan alle aanvragen, aanbiedingen en overeenkomsten waarbij OLVG optreedt als koper c.q. verwerver van goederen of diensten. Deze voorwaarden zijn op 13 september 2010 gedeponeerd bij de Kamer van Koophandel Midden Nederland en te raadplegen via www.olvg.nl/algemene_inkoopvoorwaarden From kkienitz at orclinic.com Thu Feb 18 06:55:28 2016 From: kkienitz at orclinic.com (Kienitz, Kari) Date: Thu, 18 Feb 2016 04:55:28 -0800 Subject: [Histonet] Nuclear Bubbling In-Reply-To: References: <9B1A1501A800064397369BD8072E6BCA06503B35@E2K10DB.springfieldclinic.com>, Message-ID: <41400FFE517878449D89114DD25260901C65946EAF@tocmail1.tocad.orclinic.com> incomplete deparafinization has my vote....increase times in xylene and I bet it goes away. This happened to me when the paraffin that I had bought for years was slightly changed without notifying anyone. If I remember right the polymer was increase resulting in xylene being less effective. Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz at orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: Hoekert, Willem via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, February 17, 2016 11:54 PM To: Vickroy, James; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Nuclear Bubbling Could it be due to incomplete deparaffinization? Willem ________________________________________ Van: Vickroy, James via Histonet [histonet at lists.utsouthwestern.edu] Verzonden: dinsdag 16 februari 2016 18:10 Aan: histonet at lists.utsouthwestern.edu Onderwerp: [Histonet] Nuclear Bubbling Struggling to find an answer. We do a lot of GI biopsies in our lab. Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense. Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times. I do not find that the problem is fixation. In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend). There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining. I really thought that this might be the issue however I'm not sure at this point. Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without. I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue. Here are my questions: 1. Could it be something that is happening with the tissue before it gets to the lab? Usually a delay if fixation causes other artifacts but not bubbling. Could it be heat from the GI procedure? 2. We do use blue sponges for our biopsies. I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3. What about the heat stage in our Prisma stainer? I am really getting frustrated. Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling". Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue. I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met elektronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. De algemene Inkoopvoorwaarden van de Vereniging Santeon respectievelijk de leden van de Vereniging Santeon zijn van toepassing op en maken integraal onderdeel uit van alle rechtsbetrekkingen, daaronder mede verstaan alle aanvragen, aanbiedingen en overeenkomsten waarbij OLVG optreedt als koper c.q. verwerver van goederen of diensten. Deze voorwaarden zijn op 13 september 2010 gedeponeerd bij de Kamer van Koophandel Midden Nederland en te raadplegen via www.olvg.nl/algemene_inkoopvoorwaarden _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From areichard at lmhealth.org Thu Feb 18 08:42:45 2016 From: areichard at lmhealth.org (Amanda Reichard) Date: Thu, 18 Feb 2016 09:42:45 -0500 Subject: [Histonet] Question for General Data users Message-ID: Good Morning! Does anyone else use General Data for their barcode labeling system? If you do, is your operating system the HTS system or Lab Cycle? Thanks in advance! Amanda Reichard, HTL (ASCP)cm Histology Supervisor Laboratory Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (740) 348-4163 ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From rjbuesa at yahoo.com Thu Feb 18 09:31:16 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 18 Feb 2016 15:31:16 +0000 (UTC) Subject: [Histonet] Nuclear Bubbling In-Reply-To: References: Message-ID: <217450182.4996450.1455809476672.JavaMail.yahoo@mail.yahoo.com> Willem: Essentially incomplete deparaffination shows a different pattern and is not limited to nuclei only.Again, this is what the consensus is:1- there is ALWAYS?water left underneath the section2- that water HAS to be eliminated before the section is dried in the oven3- the best sectioning practices call for placing the slides (with the just fished sections) in a vertical position resting on their?shortest end (1 inch) for a few minutes after the section is fished from the water bath. At my lab the techs were required to do that and their used to rest the sections around the water bath.4- AFTER the sections have been completely drained then they were either to the oven (60?C for at least 7 min. or to the autostainer, we used a Sakura autostainer) and the sections were stained. Which is the consensus as to what happens: when there is?water underneath the whole section?and it goes into the oven without properly drainage the HOT water will dissolve the nuclear contents something that it will NOT happen with the other tissue components (such as the connective tissue or nerves) because their chemical components are insoluble). The nuclei contain soluble chemical components and, as such, they will be dissolved in the hot water and if they are dissolved, they cannot be stained and will determine an image similar to an empty bubble, hence the "nuclear bubbling" artifact. I have?proven this mechanism?by staining sections with different degrees of drainage (measured in minutes after the sections were fished) and the results were consistent with the explanation I offered above. The "ideal" drainage time is 7 minutes. Just try it yourself to dispel any of your doubts. Ren? On Thursday, February 18, 2016 3:00 AM, "Hoekert, Willem via Histonet" wrote: Could it be due to incomplete deparaffinization? Willem ________________________________________ Van: Vickroy, James via Histonet [histonet at lists.utsouthwestern.edu] Verzonden: dinsdag 16 februari 2016 18:10 Aan: histonet at lists.utsouthwestern.edu Onderwerp: [Histonet] Nuclear Bubbling Struggling to find an answer.? We do a lot of GI biopsies in our lab.? Sometimes they look wonderful without any nuclear bubbling, other times the bubbling is pretty intense.? Since nuclear bubbling is often attributed to incomplete fixation we of course have investigated the fixation times.? I do not find that the problem is fixation.? In fact some of the biopsies end up fixing for 48 hrs before processing. (weekend).? There was a suggestion last week or so that there might be water trapped under the slides after cutting and before staining.? I really thought that this might be the issue however I'm not sure at this point.? Extra drying seems to help but sometimes slides side by side are so variable, one with bubbles and one without.? I also don't believe the problem is in the processing schedule since the problem has shown up on both a rapid and a normal schedule. (therefore longer dehydration, clearing, etc.) I am wondering if anyone else has worked with this issue.? Here are my questions: 1.? ? ? ? Could it be something that is happening with the tissue before it gets to the lab?? Usually a delay if fixation? causes other artifacts but not bubbling.? Could it be heat from the GI procedure? 2.? ? ? We do use blue sponges for our biopsies.? I know some say get rid of the sponges but has anyone seen this problem caused by usage of sponges? 3.? ? ? What about the heat stage in our Prisma stainer? I am really getting frustrated.? Pathologists never complain however I would rather all of the tissue did not have the "nuclear bubbling".? Again we only do biopsies so I really don't think the standard old " not enough time in formalin" is the issue.? I have even wondered about variables such as we use recycled formalin, recycled Clearite III. Any suggestions? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois? 62703 Office:? 217-528-7541, Ext. 15121 Email:? jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met elektronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. De algemene Inkoopvoorwaarden van de Vereniging Santeon respectievelijk de leden van de Vereniging Santeon zijn van toepassing op en maken integraal onderdeel uit van alle rechtsbetrekkingen, daaronder mede verstaan alle aanvragen, aanbiedingen en overeenkomsten waarbij OLVG optreedt als koper c.q. verwerver van goederen of diensten. Deze voorwaarden zijn op 13 september 2010 gedeponeerd bij de Kamer van Koophandel Midden Nederland en te raadplegen via www.olvg.nl/algemene_inkoopvoorwaarden _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang at gmx.at Thu Feb 18 09:50:24 2016 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 18 Feb 2016 16:50:24 +0100 Subject: [Histonet] keratohyalin granules In-Reply-To: References: Message-ID: <000b01d16a64$15d01f90$41705eb0$@gmx.at> Do you stain with hematoxylin only? or also with eosin. I think eosin is the dye, that would highlight the basic proteins in the granula. Gudrun -----Urspr?ngliche Nachricht----- Von: Kalleberg, Kristopher via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 17. Februar 2016 21:37 An: histonet at lists.utsouthwestern.edu; histonet-request at lists.utsouthwestern.edu Betreff: [Histonet] keratohyalin granules Hello All, Does anyone have experience staining the keratohyalin granules in skin with different types of hematoxylins? I am currently looking at keratohyalin granules and staining with Harris hematoxylin and there seems to be little staining and not as prominent as I would have expected. Wonder if anyone has noticed that different hematoxlins stain keratohyalin granules better than others. The skin is normal skin photoprotected and photoexposed. Thank you in advance for any helpful insights. Kris _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.Seeley at tenethealth.com Thu Feb 18 10:16:04 2016 From: Heather.Seeley at tenethealth.com (Seeley, Heather) Date: Thu, 18 Feb 2016 16:16:04 +0000 Subject: [Histonet] Lab Coats and OCT Message-ID: <9DFE334E776E734D9D231EF60CCE93C57E257FC0@TENHDCTHMB10-31.tenethealth.net> Hello All, Now that the hospital is enforcing us to wear lab coats all the time, wondering if anyone has suggestions for some that aren't too hot, the ones we have now are making us sweat! Also, we have been having issues with our OCT, we currently use the Sakura OCT and have use it for years. Within the last 6 months or so it has become like glue on the chucks even if we let it soak in hot water and scrub with a brush, it just doesn't want to come out! If anyone has any suggestions on brands to try we would appreciate it! Thanks! HEATHER SEELEY, HT(ASCP) Histotechnologist 803-985-4676 OFFICE 803-327-7598 FAX From Toni.Rathborne at rwjuh.edu Thu Feb 18 10:30:34 2016 From: Toni.Rathborne at rwjuh.edu (Rathborne, Toni) Date: Thu, 18 Feb 2016 16:30:34 +0000 Subject: [Histonet] Lab Coats and OCT In-Reply-To: <9DFE334E776E734D9D231EF60CCE93C57E257FC0@TENHDCTHMB10-31.tenethealth.net> References: <9DFE334E776E734D9D231EF60CCE93C57E257FC0@TENHDCTHMB10-31.tenethealth.net> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24332B00A9@vap1014.win.rwjuh.edu> Heather, We are currently using a lab coat made by White Knight, which is fluid resistant. I don't know if it's any cooler, but they do protect what you're wearing under it. Regarding the OCT. We also get our from Sakura, and have not noticed any change to the consistency. Is this happening with all lot numbers, or only one? I would check with Sakura. There may be a recall that you're not aware of. It might also have something to do with temperature/storage conditions. Toni -----Original Message----- From: Seeley, Heather via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 18, 2016 11:16 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Lab Coats and OCT Hello All, Now that the hospital is enforcing us to wear lab coats all the time, wondering if anyone has suggestions for some that aren't too hot, the ones we have now are making us sweat! Also, we have been having issues with our OCT, we currently use the Sakura OCT and have use it for years. Within the last 6 months or so it has become like glue on the chucks even if we let it soak in hot water and scrub with a brush, it just doesn't want to come out! If anyone has any suggestions on brands to try we would appreciate it! Thanks! HEATHER SEELEY, HT(ASCP) Histotechnologist 803-985-4676 OFFICE 803-327-7598 FAX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katherine-walters at uiowa.edu Thu Feb 18 10:39:57 2016 From: katherine-walters at uiowa.edu (Walters, Katherine S) Date: Thu, 18 Feb 2016 16:39:57 +0000 Subject: [Histonet] Lab Coats and OCT In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24332B00A9@vap1014.win.rwjuh.edu> References: <9DFE334E776E734D9D231EF60CCE93C57E257FC0@TENHDCTHMB10-31.tenethealth.net> <59E09A4EFBD3F349BD75FDAE8AFB0F24332B00A9@vap1014.win.rwjuh.edu> Message-ID: <8F5174AF8E55114E8D36E4024D8B02C41AAA4B35@hc-mailboxc1-n3.healthcare.uiowa.edu> We get our freezing medium from TBS. it is called TFM (tissue freezing medium). Lately it has become much less viscous, one of the reasons I liked it was that the viscosity allowed me to orient my tissue a little easier. The packaging also changed claiming "same TBS Product, Great New Packaging". Has anyone else noticed the change, or did I just get a bad lot? Thanks for any information, Kathy Katherine S Walters Histology Director Central Microscopy Research Facility University of Iowa 76 Eckstein Medical Research Building 431 Newton Road Iowa City, Iowa 52242 319-335-8142 Facility Website: http://cmrf.research.uiowa.edu/ -----Original Message----- From: Rathborne, Toni via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 18, 2016 10:31 AM To: 'Seeley, Heather' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Lab Coats and OCT Heather, We are currently using a lab coat made by White Knight, which is fluid resistant. I don't know if it's any cooler, but they do protect what you're wearing under it. Regarding the OCT. We also get our from Sakura, and have not noticed any change to the consistency. Is this happening with all lot numbers, or only one? I would check with Sakura. There may be a recall that you're not aware of. It might also have something to do with temperature/storage conditions. Toni -----Original Message----- From: Seeley, Heather via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 18, 2016 11:16 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Lab Coats and OCT Hello All, Now that the hospital is enforcing us to wear lab coats all the time, wondering if anyone has suggestions for some that aren't too hot, the ones we have now are making us sweat! Also, we have been having issues with our OCT, we currently use the Sakura OCT and have use it for years. Within the last 6 months or so it has become like glue on the chucks even if we let it soak in hot water and scrub with a brush, it just doesn't want to come out! If anyone has any suggestions on brands to try we would appreciate it! Thanks! HEATHER SEELEY, HT(ASCP) Histotechnologist 803-985-4676 OFFICE 803-327-7598 FAX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ From LRaff at uropartners.com Thu Feb 18 10:44:29 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 18 Feb 2016 16:44:29 +0000 Subject: [Histonet] Lab Coats (and post) Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B6D0397@COLOEXCH01.uropartners.local> Heather, We get our disposable lab coats from Choyce: Item 6250-B $94/case http://www.choyce-products.com/our-products/personal-protective-equipment/protective-clothing/lab-coats.html ------------------------------------ Latest blog post: http://www.chicagonow.com/downsize-maybe/2016/02/would-we-still-want-to-be-on-family-feud/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From brett_connolly at merck.com Thu Feb 18 11:24:43 2016 From: brett_connolly at merck.com (Connolly, Brett M) Date: Thu, 18 Feb 2016 12:24:43 -0500 Subject: [Histonet] Lab Coats and OCT In-Reply-To: <9DFE334E776E734D9D231EF60CCE93C57E257FC0@TENHDCTHMB10-31.tenethealth.net> References: <9DFE334E776E734D9D231EF60CCE93C57E257FC0@TENHDCTHMB10-31.tenethealth.net> Message-ID: I am a fan of Cryo-Gel - highly viscous which facilitates easier tissue orientation. We get it from Electron Microscopy Sciences (#62806-01). Try it, you'll like it. Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: Seeley, Heather via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 18, 2016 11:16 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Lab Coats and OCT Hello All, Now that the hospital is enforcing us to wear lab coats all the time, wondering if anyone has suggestions for some that aren't too hot, the ones we have now are making us sweat! Also, we have been having issues with our OCT, we currently use the Sakura OCT and have use it for years. Within the last 6 months or so it has become like glue on the chucks even if we let it soak in hot water and scrub with a brush, it just doesn't want to come out! If anyone has any suggestions on brands to try we would appreciate it! Thanks! HEATHER SEELEY, HT(ASCP) Histotechnologist 803-985-4676 OFFICE 803-327-7598 FAX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From histo at pathlab.us Thu Feb 18 12:46:47 2016 From: histo at pathlab.us (Histology) Date: Thu, 18 Feb 2016 18:46:47 +0000 Subject: [Histonet] Herpes Zoster (VZV) Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD82315AFF@vdc01.domain.local> Hi everyone- Will anyone share where they get their Herpes Zoster antibody from for FFPE? I currently get it from abcam and run them on a Ventana Ultra. It seems once a year or so, the antibody just stops working and I have to call abcam and they send me a new one and it usually works. This is the only antibody that I have this problem with and I'm not sure what's happening. Any help would be great. Thanks, Mehndi Helgren "DPL" From esarricks at gmail.com Thu Feb 18 13:09:57 2016 From: esarricks at gmail.com (Erin Sarricks) Date: Thu, 18 Feb 2016 14:09:57 -0500 Subject: [Histonet] Microm Block Holder Message-ID: Hi Histonet- I am searching for a block holder for our Microm microtome and am wondering if anyone has one they are willing to part with or sell. Thanks in advance for your help! Erin From wdesalvo.cac at outlook.com Thu Feb 18 16:40:14 2016 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Thu, 18 Feb 2016 15:40:14 -0700 Subject: [Histonet] Bar Code and Tracking Message-ID: I believe it will be difficult to afford the cost of Co-Path in your small lab area, unless you can attach to a bigger hospital information system contract. I suggest you look to companies that have developed products for the small to medium sized histology labs. Sent from my Windows Phone ________________________________ From: Abbott, Tanya via Histonet Sent: ?2/?17/?2016 11:48 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Bar Code and Tracking Hello, I manage a small Pathology lab where we generate approx. 80-140 blocks for Histo and 5-6 Cytology fluids a day. I have 2 Histotechs that swap doing grossing monthly. I have 1 full time and 1 part time Cytotech and a Cyto assistant. I am curious if there are any smaller labs out there that have gone to a Bar Code and Tracking system and can offer any input. I came from a much larger lab that used CoPath from accessioning to sign-out, so that is the kind of system I am used to. It is certainly a system I wish to integrate in to my lab on a much smaller level, we currently have a very manual process, other than some entry/labeling in our LIS. Any input is appreciated! Tanya Tanya G. Abbott Pathology Manager Penn State Health St. Joseph Pathology Department (o) 610-378-2635 (f) 670-898-5871 tabbott at pennstatehealth.psu.edu Penn State Health St. Joseph | The Future of Healthcare _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vapatpxs at yahoo.com Thu Feb 18 18:46:57 2016 From: vapatpxs at yahoo.com (Va Paula Sicurello) Date: Fri, 19 Feb 2016 00:46:57 +0000 (UTC) Subject: [Histonet] 10% NBF Question References: <1600416797.6133274.1455842817515.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1600416797.6133274.1455842817515.JavaMail.yahoo@mail.yahoo.com> Hello Listers, Has anyone had any problems with fixation using Richard-Allan Scientific 10% NBF? Some of our gastric biopsies are looking a little off. The rest of the biopsies fixed in the same NBF and processed on the same processor look fine. This issue would have come up in the last couple of weeks. I know that Richard-Allan recalled their NBF a couple of years ago because the % was 0-3 not 10%. Please let me know if any of you are experiencing something similar. Sincerely, Paula Paula Sicurello Histotechnology Specialist UC San Diego Health From Fawn.Bomar at HalifaxRegional.com Fri Feb 19 08:36:41 2016 From: Fawn.Bomar at HalifaxRegional.com (Fawn Bomar) Date: Fri, 19 Feb 2016 14:36:41 +0000 Subject: [Histonet] Lead Tech/ Supervisor Competency Message-ID: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA1A206C@EXCH-2K10.hrhs.com> Hi all, I didn't receive any responses in regards to my last post so I thought I would try one more time. Does anyone have a competency or orientation checklist that they use for lead techs or supervisors that they would be willing to share? On my last post, everyone that messaged asked for copies of my assessment and the assessments that were sent to me, which I did send to everyone that requested them. If anyone else would like me to send my assessment to the them to look at, please let me know and I will be happy to share, plus I would be appreciative of any feedback- positive or negative- and any suggestions for improvement. I tried to send an attachment of my assessment to the histonet but it kept saying it was waiting for moderator approval and it never was sent. Thank you all for your help and guidance. Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From j.rowaihi at alborglaboratories.com Fri Feb 19 09:22:38 2016 From: j.rowaihi at alborglaboratories.com (Jamal Rowaihi) Date: Fri, 19 Feb 2016 18:22:38 +0300 Subject: [Histonet] Lead Tech/ Supervisor Competency Message-ID: Thanks FawanI think some are not completely satisfied with their papers that's why they didn't respond to us, but I think we suppose to share our experience to improve the quality, soon I will send you my own and I hope you send me yours... Good luck.? Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories?Sent from my cell phone-------- Original message --------From: Fawn Bomar via Histonet Date: 2/19/2016 5:36 PM (GMT+03:00) To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Lead Tech/ Supervisor Competency Hi all, I didn't receive any responses in regards to my last post so I thought I would try one more time.? Does anyone have a competency or orientation checklist that they use for lead techs or supervisors that they would be willing to share?? On my last post, everyone that messaged asked for copies of my assessment and the assessments that were sent to me, which I did send to everyone that requested them.? If anyone else would like me to send my assessment to the them to look at, please let me know and I will be happy to share, plus I would be appreciative of any feedback- positive or negative- and any suggestions for improvement.? I tried to send an attachment of my assessment to the histonet but it kept saying it was waiting for moderator approval and it never was sent. Thank you all for your help and guidance. Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged.? It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aarmstrong at statlab.com Fri Feb 19 10:06:43 2016 From: aarmstrong at statlab.com (Amy Armstrong) Date: Fri, 19 Feb 2016 16:06:43 +0000 Subject: [Histonet] Need Slide Cabinets Message-ID: Hi Rayen, We have the metal slide cabinets. Please let me know if you are still interested. Thank you, Amy Amy Armstrong StatLab Medical Products Account Manager 2090 Commerce Drive | McKinney, TX 75069 t: 214.868.7164 | f: 972.436.1369 aarmstrong at statlab.com | www.statlab.com From MWhite at mhs.net Fri Feb 19 12:06:41 2016 From: MWhite at mhs.net (White, Marcia) Date: Fri, 19 Feb 2016 18:06:41 +0000 Subject: [Histonet] Bar Code and Tracking In-Reply-To: References: Message-ID: <8B4B5BF3FCB6D24CBEAA63ACA5FDAD3B55F17952@MHSEXMB07.mhs.net> Try Computer Trust Corporation, they are much smaller than the larger companies and customize everything to meet your needs Marcia M White Pathology Manager Memorial Regional Hospital 954-265-5371-office 954-967-7627-fax MWhite at mhs.net -----Original Message----- From: WILLIAM DESALVO [mailto:wdesalvo.cac at outlook.com] Sent: Thursday, February 18, 2016 5:40 PM To: Abbott, Tanya; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Bar Code and Tracking I believe it will be difficult to afford the cost of Co-Path in your small lab area, unless you can attach to a bigger hospital information system contract. I suggest you look to companies that have developed products for the small to medium sized histology labs. Sent from my Windows Phone ________________________________ From: Abbott, Tanya via Histonet Sent: ?2/?17/?2016 11:48 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Bar Code and Tracking Hello, I manage a small Pathology lab where we generate approx. 80-140 blocks for Histo and 5-6 Cytology fluids a day. I have 2 Histotechs that swap doing grossing monthly. I have 1 full time and 1 part time Cytotech and a Cyto assistant. I am curious if there are any smaller labs out there that have gone to a Bar Code and Tracking system and can offer any input. I came from a much larger lab that used CoPath from accessioning to sign-out, so that is the kind of system I am used to. It is certainly a system I wish to integrate in to my lab on a much smaller level, we currently have a very manual process, other than some entry/labeling in our LIS. Any input is appreciated! Tanya Tanya G. Abbott Pathology Manager Penn State Health St. Joseph Pathology Department (o) 610-378-2635 (f) 670-898-5871 tabbott at pennstatehealth.psu.edu Penn State Health St. Joseph | The Future of Healthcare _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. From ASelf at tidelandshealth.org Fri Feb 19 13:45:31 2016 From: ASelf at tidelandshealth.org (Amy Self) Date: Fri, 19 Feb 2016 14:45:31 -0500 Subject: [Histonet] CPT Code 88399 Message-ID: Happy Friday and I hope everyone has a great weekend, Can anyone tell me what the CPT code 88399 can be used for in pathology? We have been using this code to track mail-outs for productivity purposes but was told today that because there is not a dollar amount attached to this code that we could no longer use it. Is there a cpt code that can be used for when sending pathology material out to other facilities when the patient is being seen by a physician for continued healthcare? Thanks in advance for you help, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From plucas at biopath.org Fri Feb 19 14:31:32 2016 From: plucas at biopath.org (Paula) Date: Fri, 19 Feb 2016 12:31:32 -0800 Subject: [Histonet] BD Surepath VS Holgic Thinprep for Non-gyn speciemens Message-ID: <000001d16b54$8609a920$921cfb60$@biopath.org> Hello, We're looking for a lab in the Orange County, California area that is preparing non-gyn samples using the Surepath system. We would like to submit a sample to evaluate the BD Prep stain slide processor for non-gyn specimens. We would like to compare this to the Hologic's Thinprep system, which we have in place. If you are willing to test a sample for us to compare, that would be great. Please call or reply to this email. Thank you, Paula Lucas-HT Lab Manager BioPath Medical Group Fountain Valley, CA 92708 714-433-1330 From jford at cytomx.com Fri Feb 19 18:53:37 2016 From: jford at cytomx.com (Judi Ford) Date: Sat, 20 Feb 2016 00:53:37 +0000 Subject: [Histonet] Double stain IHC question Message-ID: Hi everyone, I have a question in chromogenic double staining. Here is the situation. Tissue = human, frozen Antibody = same protein (A) 1. Commercial antibody of A 2. Homegrown antibody of A, human, biotinylated Question: can you stain both versions of this antibody on the same tissue, same slide? Goal is to see where each stains in the tissue and if they co-localize. If they do co-localize then how do you distinguish between that and where they stain individually? Would you use different chromogens and hope that where they come together it turns a different color? I am really interested if this can work. Thanks in advance for any replies. Judi South San Francisco, CA From gu.lang at gmx.at Sat Feb 20 05:47:05 2016 From: gu.lang at gmx.at (Gudrun Lang) Date: Sat, 20 Feb 2016 12:47:05 +0100 Subject: [Histonet] Double stain IHC question In-Reply-To: References: Message-ID: <000001d16bd4$6e7ed660$4b7c8320$@gmx.at> In my opinion, this would only be possible, if the commercial and the homegrown antibody are from different species. For example one from mouse and one from rabbit. Then you can proceed with different secondaries (goat anti mouse conjugated with peroxidase, goat anti rabbit conjugated with alkaline phosphatase). Then chromogens that work with each of the enzymes. If the antibodies are from the same species I see no way to distinguish both. Only if one is conjugated with biotin and the other with digoxigenin, then you could proceed with secondaries against biotin and digoxigenin. etc.. Gudrun -----Urspr?ngliche Nachricht----- Von: Judi Ford via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Samstag, 20. Februar 2016 01:54 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Double stain IHC question Hi everyone, I have a question in chromogenic double staining. Here is the situation. Tissue = human, frozen Antibody = same protein (A) 1. Commercial antibody of A 2. Homegrown antibody of A, human, biotinylated Question: can you stain both versions of this antibody on the same tissue, same slide? Goal is to see where each stains in the tissue and if they co-localize. If they do co-localize then how do you distinguish between that and where they stain individually? Would you use different chromogens and hope that where they come together it turns a different color? I am really interested if this can work. Thanks in advance for any replies. Judi South San Francisco, CA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mills at 3scan.com Sat Feb 20 10:35:18 2016 From: mills at 3scan.com (Caroline Miller) Date: Sat, 20 Feb 2016 08:35:18 -0800 Subject: [Histonet] Double stain IHC question In-Reply-To: <000001d16bd4$6e7ed660$4b7c8320$@gmx.at> References: <000001d16bd4$6e7ed660$4b7c8320$@gmx.at> Message-ID: + to Gudrun's comments. My addition is that fluorescence labels may work for this application. Again, depending on the species of the antibodies. The other advantage of the fluorescence is that you would be able to truly see the colocalization (yellow for instance if you used red and green fluorescent antibodies). Rather than the muddiness I have often got when trying two antibodies in bright field chromagenic stains. Remember though autofluorescence is greatly increased with paraffin tissues, that may put another issue on your plate. Good luck! yours, mills On Sat, Feb 20, 2016 at 3:47 AM, Gudrun Lang via Histonet < histonet at lists.utsouthwestern.edu> wrote: > In my opinion, this would only be possible, if the commercial and the > homegrown antibody are from different species. For example one from mouse > and one from rabbit. > Then you can proceed with different secondaries (goat anti mouse conjugated > with peroxidase, goat anti rabbit conjugated with alkaline phosphatase). > Then chromogens that work with each of the enzymes. > > If the antibodies are from the same species I see no way to distinguish > both. Only if one is conjugated with biotin and the other with digoxigenin, > then you could proceed with secondaries against biotin and digoxigenin. > etc.. > > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: Judi Ford via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Gesendet: Samstag, 20. Februar 2016 01:54 > An: histonet at lists.utsouthwestern.edu > Betreff: [Histonet] Double stain IHC question > > Hi everyone, > I have a question in chromogenic double staining. Here is the situation. > Tissue = human, frozen > Antibody = same protein (A) > > 1. Commercial antibody of A > > 2. Homegrown antibody of A, human, biotinylated > > Question: can you stain both versions of this antibody on the same tissue, > same slide? Goal is to see where each stains in the tissue and if they > co-localize. If they do co-localize then how do you distinguish between > that > and where they stain individually? Would you use different chromogens and > hope that where they come together it turns a different color? > I am really interested if this can work. Thanks in advance for any replies. > Judi > South San Francisco, CA > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From dblackburn2000 at yahoo.com Sat Feb 20 13:44:32 2016 From: dblackburn2000 at yahoo.com (daniel blackburn) Date: Sat, 20 Feb 2016 19:44:32 +0000 (UTC) Subject: [Histonet] question - Allergy to histological solvents? References: <145296661.7458743.1455997472713.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <145296661.7458743.1455997472713.JavaMail.yahoo@mail.yahoo.com> Hello After decades of using standard organic solvents for paraffin- section histology, I find that I've become highly allergic to the fumes. These include commercial preps like Citrosolv and Hemo-De, as well as xylene and toluene, which cause (in me) dizziness and a pounding headache. This problem has made staining and coverslipping very difficult, even with hood. Can anyone recommend alternative solutions for (a) dewaxing prior to staining and (b) coverslipping? thanks very much! Daniel Blackburn Biology, Trinity College (Hartford CT) From kdwyatt at vet.k-state.edu Sat Feb 20 16:54:48 2016 From: kdwyatt at vet.k-state.edu (Kristina Wyatt) Date: Sat, 20 Feb 2016 22:54:48 +0000 Subject: [Histonet] Positive Streptococcus pneumoniae IHC controls Message-ID: Does anyone know of a commercial positive control tissue for S. pneumoniae? We have a pig study and need an antibody along with a known positive control to run optimization tests. So far, I have a reference for the antibody used in mouse models, but can't track down positive tissue for a control. Thanks, Kristina Wyatt, M.A. Supervisor: Histopathology/Immunohistochemistry L216 Mosier Hall Kansas State Veterinary Diagnostic Laboratory 785-532-4464 kdwyatt at vet.k-state.edu From abtdhu at gmail.com Sat Feb 20 17:02:45 2016 From: abtdhu at gmail.com (abtdhu at gmail.com) Date: Sat, 20 Feb 2016 18:02:45 -0500 Subject: [Histonet] Histonet Digest, Vol 147, Issue 21"............Double stain IHC question In-Reply-To: References: Message-ID: I wonder if streptavidin Alexa (495 or 488) can be use to bind to biotinlyted primary. So no worry about species issue? May need to do biotin blocking first to get ride of endogenic biotin I guess. Dorothy Hu Sent from my iPad > On Feb 20, 2016, at 1:00 PM, histonet-request at lists.utsouthwestern.edu wrote: > > Double stain IHC question From koellingr at comcast.net Sat Feb 20 17:39:27 2016 From: koellingr at comcast.net (koellingr at comcast.net) Date: Sat, 20 Feb 2016 23:39:27 +0000 (UTC) Subject: [Histonet] Double stain IHC question In-Reply-To: References: <000001d16bd4$6e7ed660$4b7c8320$@gmx.at> Message-ID: <821436208.2712920.1456011567745.JavaMail.zimbra@comcast.net> All, I guess I'm reading (or misreading) the situation differently than some.? That this is frozen, and not FFPE, is given originally. ? But I'm reading this as two different antibodies marking the very same protein. [Antibody=same protein A].? Is the original question can you mark same protein with two different antibodies to it?? I have done this with two antibodies to DIFFERENT epitopes of the same protein from home grown antibodies and we knew which antibody was directed to which epitope.? If two different antibodies are directed to the SAME epitope on the same protein they will just compete and favor that one with greater affinity/avidity/more conducive physical characteristics for binding.? Then colocalizing with fluorochromes is certainly possible as is done routinely. ? Ray (now in Spokane WA) ----- Original Message ----- From: "Caroline Miller via Histonet" To: "gu lang" Cc: "Histonet at Lists. Edu" Sent: Saturday, February 20, 2016 8:35:18 AM Subject: Re: [Histonet] Double stain IHC question + to Gudrun's comments. My addition is that fluorescence labels may work for this application. Again, depending on the species of the antibodies. The other advantage of the fluorescence is that you would be able to truly see the colocalization (yellow for instance if you used red and green fluorescent antibodies). Rather than the muddiness I have often got when trying two antibodies in bright field chromagenic stains. Remember though autofluorescence is greatly increased with paraffin tissues, that may put another issue on your plate. Good luck! yours, mills On Sat, Feb 20, 2016 at 3:47 AM, Gudrun Lang via Histonet < histonet at lists.utsouthwestern.edu> wrote: > In my opinion, this would only be possible, if the commercial and the > homegrown antibody are from different species. For example one from mouse > and one from rabbit. > Then you can proceed with different secondaries (goat anti mouse conjugated > with peroxidase, goat anti rabbit conjugated with alkaline phosphatase). > Then chromogens that work with each of the enzymes. > > If the antibodies are from the same species I see no way to distinguish > both. Only if one is conjugated with biotin and the other with digoxigenin, > then you could proceed with secondaries against biotin and digoxigenin. > etc.. > > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: Judi Ford via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Gesendet: Samstag, 20. Februar 2016 01:54 > An: histonet at lists.utsouthwestern.edu > Betreff: [Histonet] Double stain IHC question > > Hi everyone, > I have a question in chromogenic double staining. Here is the situation. > ? ? ? ? ? ? ? ? Tissue = human, frozen > ? ? ? ? ? ? ? ? Antibody = same protein (A) > > 1. ? ? ? Commercial antibody of A > > 2. ? ? ? Homegrown antibody of A, human, biotinylated > > Question: can you stain both versions of this antibody on the same tissue, > same slide? Goal is to see where each stains in the tissue and if they > co-localize. If they do co-localize then how do you distinguish between > that > and where they stain individually? Would you use different chromogens and > hope that where they come together it turns a different color? > I am really interested if this can work. Thanks in advance for any replies. > Judi > South San Francisco, CA > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From susanhay59 at gmail.com Sat Feb 20 22:12:45 2016 From: susanhay59 at gmail.com (S hay) Date: Sat, 20 Feb 2016 22:12:45 -0600 Subject: [Histonet] CPT Code 88399 In-Reply-To: References: Message-ID: Please post answers to the group. We would like to see the responses as well. On Feb 19, 2016 2:04 PM, "Amy Self via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > Happy Friday and I hope everyone has a great weekend, > > Can anyone tell me what the CPT code 88399 can be used for in pathology? > > We have been using this code to track mail-outs for productivity purposes > but was told today that because there is not a dollar amount attached to > this code that we could no longer use it. > > Is there a cpt code that can be used for when sending pathology material > out to other facilities when the patient is being seen by a physician for > continued healthcare? > > Thanks in advance for you help, > Amy Self > Histology Lab Senior Tech > Lab > Tidelands Georgetown Memorial Hospital > 606 Black River Road > Georgetown, SC 29440 > 843-520-8711 > ASelf at tidelandshealth.org > > NOTE: > The information contained in this message may be privileged, confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to this message and deleting it from your > computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From steven.weston at utas.edu.au Sun Feb 21 01:35:57 2016 From: steven.weston at utas.edu.au (Steven Weston) Date: Sun, 21 Feb 2016 07:35:57 +0000 Subject: [Histonet] Double stain IHC question Message-ID: <1456040156260.22786@utas.edu.au> This is probably most easily done using immunofluorescence since it works well on frozen tissue. You could use a streptavidin linked FITC tag for the human biotinylated Ab, and then use a red tagged anti IgG directed against the species of the other antibody. (hoescht reagent will stain the nuclei) If however you want to use immunohistochemistry you can use a strepatvidin linked alkaline phosphatase with BCIP/NBT (blue chomogen) for the biotinylated Ab and a polymer based peroxidase secondary for the other antibody and visualise using DAB. For nuclear staining you can use nuclear fast red. I have used this method recently to stain cytospots successfully for similar macrophage markers (inos and cd68). Hope this helps. regards steve weston lab manager Breathe-Well CRE UTAS-SOM University of Tasmania Electronic Communications Policy (December, 2014). This email is confidential, and is for the intended recipient only. Access, disclosure, copying, distribution, or reliance on any of it by anyone outside the intended recipient organisation is prohibited and may be a criminal offence. Please delete if obtained in error and email confirmation to the sender. The views expressed in this email are not necessarily the views of the University of Tasmania, unless clearly intended otherwise. From rjbuesa at yahoo.com Sun Feb 21 09:29:09 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Sun, 21 Feb 2016 15:29:09 +0000 (UTC) Subject: [Histonet] question - Allergy to histological solvents? In-Reply-To: <145296661.7458743.1455997472713.JavaMail.yahoo@mail.yahoo.com> References: <145296661.7458743.1455997472713.JavaMail.yahoo@mail.yahoo.com> Message-ID: <365183885.459796.1456068549978.JavaMail.yahoo@mail.yahoo.com> You can dewax absolutely safely using a 2% dishawasher soap solution at 90?C (twice) as washing in water.You can "dehydrate" stained stains by placing the slides in an oven at 60?C, also absolutely safely for the stained section.Under separate cover I am sending? articles on this subject.Ren? On Saturday, February 20, 2016 3:05 PM, daniel blackburn via Histonet wrote: Hello After decades of using standard organic solvents for paraffin- section histology, I find that I've become highly allergic to the fumes.? These include commercial preps like Citrosolv and Hemo-De, as well as xylene and toluene, which cause (in me) dizziness and a pounding headache.? This problem has made staining and coverslipping very difficult, even with? hood.? Can anyone recommend alternative solutions for (a) dewaxing prior to staining and (b) coverslipping?? thanks very much! Daniel Blackburn Biology, Trinity College (Hartford CT) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM at gru.edu Mon Feb 22 09:24:05 2016 From: BZIMMERM at gru.edu (Zimmerman, Billie) Date: Mon, 22 Feb 2016 15:24:05 +0000 Subject: [Histonet] Georgia Society for Histology Annual Histopalooza Message-ID: Spring is just around the corner and GSH cordially invites you to our annual meeting at the Lanier Islands resort. The Lakefront Lodge is 12 miles from the Mall of Georgia and offers many amenities, such as free wifi and heated outdoor pool. There's no better way to earn your CE credits. It's reasonably priced, beautiful location, and convenient for the shopaholics as well. Check it out: http://www.lanierislands.com/accommodations/lodge?rt=google|cpc|LAL01-Lanier-Islands-Brand-Drive-MKT|lake%20lanier%20lodge&gclid=CLrx0aPUi8sCFdgegQod9IIHlQ Billie Zimmerman MT(ASCP)QIHC GSH Secretary From gu.lang at gmx.at Mon Feb 22 09:59:21 2016 From: gu.lang at gmx.at (Gudrun Lang) Date: Mon, 22 Feb 2016 16:59:21 +0100 Subject: [Histonet] formalin and shrinkage Message-ID: <000601d16d89$ffa29fd0$fee7df70$@gmx.at> Hi! Today someone asked me about shrinkage caused by the fixation with formaldehyde specially on skin-biopsies. She spoke about shrinkage of 30% percent. In my opinion shrinkage is mainly caused by the processing with dehydration and defatting. Formaldehyde renders the tissue harder but not strictly smaller. What is the opinion of the community? Gudrun From jford at cytomx.com Mon Feb 22 11:45:20 2016 From: jford at cytomx.com (Judi Ford) Date: Mon, 22 Feb 2016 17:45:20 +0000 Subject: [Histonet] Davidson's fixative and IHC question Message-ID: Hi Everyone. Hope you all had a really good weekend. Thanks for the replies to my double ihc question. I do have another question; this one is from a friend of mine. Her client wants to do ihc on rabbit eye tissue. The tissue will be fixed in Davidson's fixative for 24 hours then in 10% NBF. Will this have affect future ihc projects? Thanks, Judi From jaylundgren at gmail.com Mon Feb 22 13:30:46 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Mon, 22 Feb 2016 13:30:46 -0600 Subject: [Histonet] formalin and shrinkage In-Reply-To: <000601d16d89$ffa29fd0$fee7df70$@gmx.at> References: <000601d16d89$ffa29fd0$fee7df70$@gmx.at> Message-ID: I was taught at AFIP to expect shrinkage of 10%, in each dimension. So I guess that's 30% shrinkage overall? Shrinkage is partially caused by formalin crosslinking the proteins in fixation, and partially by dehydration. Maybe a little shrinkage in xylene too? From removal of fat in adipose tissues? http://link.springer.com/article/10.1007/BF00695061#page-1 Is your Pathologist really concerned about shrinkage, or about curling and distortion of small shave bxs? Because a certain degree of shrinkage is an unavoidable artifact of tissue processing. If it's the latter, I like to use 2 blue sponges. I find they really help to keep things flat and oriented. Some people don't like them because of carryover. I just say change your processor reagents more often. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Feb 22, 2016 at 9:59 AM, Gudrun Lang via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi! > > Today someone asked me about shrinkage caused by the fixation with > formaldehyde specially on skin-biopsies. She spoke about shrinkage of 30% > percent. In my opinion shrinkage is mainly caused by the processing with > dehydration and defatting. Formaldehyde renders the tissue harder but not > strictly smaller. > > > > What is the opinion of the community? > > > > Gudrun > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mjones at metropath.com Mon Feb 22 13:48:20 2016 From: mjones at metropath.com (Michael Ann Jones) Date: Mon, 22 Feb 2016 19:48:20 +0000 Subject: [Histonet] formalin and shrinkage In-Reply-To: References: <000601d16d89$ffa29fd0$fee7df70$@gmx.at> Message-ID: Agree with you Jay Lundgren. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com On 2/22/16, 12:30 PM, "Jay Lundgren via Histonet" wrote: >I was taught at AFIP to expect shrinkage of 10%, in each dimension. So I >guess that's 30% shrinkage overall? Shrinkage is partially caused by >formalin crosslinking the proteins in fixation, and partially by >dehydration. Maybe a little shrinkage in xylene too? From removal of fat >in adipose tissues? >http://link.springer.com/article/10.1007/BF00695061#page-1 > >Is your Pathologist really concerned about shrinkage, or about curling and >distortion of small shave bxs? Because a certain degree of shrinkage is >an >unavoidable artifact of tissue processing. > >If it's the latter, I like to use 2 blue sponges. I find they really help >to keep things flat and oriented. Some people don't like them because of >carryover. I just say change your processor reagents more often. > >Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > > >On Mon, Feb 22, 2016 at 9:59 AM, Gudrun Lang via Histonet < >histonet at lists.utsouthwestern.edu> wrote: > >> Hi! >> >> Today someone asked me about shrinkage caused by the fixation with >> formaldehyde specially on skin-biopsies. She spoke about shrinkage of >>30% >> percent. In my opinion shrinkage is mainly caused by the processing with >> dehydration and defatting. Formaldehyde renders the tissue harder but >>not >> strictly smaller. >> >> >> >> What is the opinion of the community? >> >> >> >> Gudrun >> >> >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren at gmail.com Mon Feb 22 13:53:09 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Mon, 22 Feb 2016 13:53:09 -0600 Subject: [Histonet] Davidson's fixative and IHC question In-Reply-To: References: Message-ID: Today I learned what Davidson's fixative is! Thanks! Anyway, so it's a strong alcoholic formalin. The answer to your question is; yes. You need to be careful of overfixation, thus masking the epitope in question. >From Dako: " Of the many pre-analytical variables which affect IHC and ISH results, fixation is probably the most significant, impacting many other variables such as antigen retrieval and epitope binding. Unfortunately, to date, no single fixative has proven to be ideal for all targets and detection methods. However, it is generally more deleterious for tissue to be ?underfixed?, rather than ?overfixed?." Make sure not to leave your specimen in Davidson's for more than 24 hrs, and then in formalin for *less* than 72 hrs and you should be OK. If staining is unsatisfactory you might have to tinker with your antigen retrieval protocol (longer) or primary Ab incubation time (longer). Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Feb 22, 2016 at 11:45 AM, Judi Ford via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Everyone. Hope you all had a really good weekend. Thanks for the > replies to my double ihc question. > > I do have another question; this one is from a friend of mine. Her client > wants to do ihc on rabbit eye tissue. The tissue will be fixed in > Davidson's fixative for 24 hours then in 10% NBF. Will this have affect > future ihc projects? > > Thanks, > Judi > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang at gmx.at Mon Feb 22 14:16:40 2016 From: gu.lang at gmx.at (Gudrun Lang) Date: Mon, 22 Feb 2016 21:16:40 +0100 Subject: [Histonet] formalin and shrinkage In-Reply-To: References: <000601d16d89$ffa29fd0$fee7df70$@gmx.at> Message-ID: <000901d16dad$f1357fc0$d3a07f40$@gmx.at> My question refers to the difference in dimensions of the native tissue and the fixed tissue. ? so without any influence of ethanol and xylol. I have no access to the whole publication you mentioned. If I understand the summary correctly, 2,5% shrinkage was found after formalinfixation. There is no actual problem to solve, just academic question. Gudrun Von: Jay Lundgren [mailto:jaylundgren at gmail.com] Gesendet: Montag, 22. Februar 2016 20:31 An: gu.lang at gmx.at Cc: histonet Betreff: Re: [Histonet] formalin and shrinkage I was taught at AFIP to expect shrinkage of 10%, in each dimension. So I guess that's 30% shrinkage overall? Shrinkage is partially caused by formalin crosslinking the proteins in fixation, and partially by dehydration. Maybe a little shrinkage in xylene too? From removal of fat in adipose tissues? http://link.springer.com/article/10.1007/BF00695061#page-1 Is your Pathologist really concerned about shrinkage, or about curling and distortion of small shave bxs? Because a certain degree of shrinkage is an unavoidable artifact of tissue processing. If it's the latter, I like to use 2 blue sponges. I find they really help to keep things flat and oriented. Some people don't like them because of carryover. I just say change your processor reagents more often. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Feb 22, 2016 at 9:59 AM, Gudrun Lang via Histonet wrote: Hi! Today someone asked me about shrinkage caused by the fixation with formaldehyde specially on skin-biopsies. She spoke about shrinkage of 30% percent. In my opinion shrinkage is mainly caused by the processing with dehydration and defatting. Formaldehyde renders the tissue harder but not strictly smaller. What is the opinion of the community? Gudrun _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang at gmx.at Mon Feb 22 14:21:23 2016 From: gu.lang at gmx.at (Gudrun Lang) Date: Mon, 22 Feb 2016 21:21:23 +0100 Subject: [Histonet] Davidson's fixative and IHC question In-Reply-To: References: Message-ID: <000e01d16dae$9a6b10a0$cf4131e0$@gmx.at> I think fixing with Davidson is ethanol-fixation in the first line. There are some antigens, that are susceptible to ethanol. Gudrun -----Urspr?ngliche Nachricht----- Von: Judi Ford via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Montag, 22. Februar 2016 18:45 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Davidson's fixative and IHC question Hi Everyone. Hope you all had a really good weekend. Thanks for the replies to my double ihc question. I do have another question; this one is from a friend of mine. Her client wants to do ihc on rabbit eye tissue. The tissue will be fixed in Davidson's fixative for 24 hours then in 10% NBF. Will this have affect future ihc projects? Thanks, Judi _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Mon Feb 22 15:10:17 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 22 Feb 2016 21:10:17 +0000 Subject: [Histonet] Shrinkage Message-ID: <48E053DDF6CE074DB6A7414BA05403F8061B73@HRHEX02-HOS.holyredeemer.local> LOL...Shrinkage...heh, heh. But seriously, there should be little to no gross shrinkage from formalin fixation and if the specimen is properly fixed, then there should be very little gross shrinkage as it is dehydrated. That is supposed to be the point! If someone is getting 30% shrinkage, there is something seriously wrong with their processing schedule. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Today's Topics: 2. formalin and shrinkage (Gudrun Lang) Message: 2 Date: Mon, 22 Feb 2016 16:59:21 +0100 From: "Gudrun Lang" Subject: [Histonet] formalin and shrinkage Hi! Today someone asked me about shrinkage caused by the fixation with formaldehyde specially on skin-biopsies. She spoke about shrinkage of 30% percent. In my opinion shrinkage is mainly caused by the processing with dehydration and defatting. Formaldehyde renders the tissue harder but not strictly smaller. What is the opinion of the community? Gudrun From akbitting at geisinger.edu Mon Feb 22 15:39:14 2016 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Mon, 22 Feb 2016 21:39:14 +0000 Subject: [Histonet] BCL-10 for IHC-P Message-ID: <7156b3cb49c64e83b87b9168cf6ac777@LOFEXMBX110W12V.geisinger.edu> Has anyone been able to optimize an antibody to BCL-10 using Ventana's Ultraview detection? Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From mills at 3scan.com Mon Feb 22 15:41:05 2016 From: mills at 3scan.com (Caroline Miller) Date: Mon, 22 Feb 2016 13:41:05 -0800 Subject: [Histonet] Shrinkage In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8061B73@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8061B73@HRHEX02-HOS.holyredeemer.local> Message-ID: hi All, I have done some experiments in this area for mouse brains, and I find that there is actually an expansion of tissue after formalin fixation (around 10%), but then certainly a shrinkage to 100% dehydration agent of about 20% from the original size. We found similar results with alcohol, acetone and THF. This is manual fluid changes or a day per solution, with no vacuum, temp or pressure. Happy to share the data if anyone is interested yours, mills On Mon, Feb 22, 2016 at 1:10 PM, Terri Braud via Histonet < histonet at lists.utsouthwestern.edu> wrote: > LOL...Shrinkage...heh, heh. > But seriously, there should be little to no gross shrinkage from formalin > fixation and if the specimen is properly fixed, then there should be very > little gross shrinkage as it is dehydrated. That is supposed to be the > point! If someone is getting 30% shrinkage, there is something seriously > wrong with their processing schedule. > Sincerely, Terri > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > > Today's Topics: > 2. formalin and shrinkage (Gudrun Lang) > > > Message: 2 > Date: Mon, 22 Feb 2016 16:59:21 +0100 > From: "Gudrun Lang" > Subject: [Histonet] formalin and shrinkage > Hi! > Today someone asked me about shrinkage caused by the fixation with > formaldehyde specially on skin-biopsies. She spoke about shrinkage of 30% > percent. In my opinion shrinkage is mainly caused by the processing with > dehydration and defatting. Formaldehyde renders the tissue harder but not > strictly smaller. > What is the opinion of the community? > Gudrun > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From cforster at umn.edu Mon Feb 22 15:54:40 2016 From: cforster at umn.edu (Colleen Forster) Date: Mon, 22 Feb 2016 15:54:40 -0600 Subject: [Histonet] Shrinkage In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F8061B73@HRHEX02-HOS.holyredeemer.local> Message-ID: I find the same as Caroline Miller, about 20-25% with formalin fixation and routine processing. C On Mon, Feb 22, 2016 at 3:41 PM, Caroline Miller via Histonet < histonet at lists.utsouthwestern.edu> wrote: > hi All, I have done some experiments in this area for mouse brains, and I > find that there is actually an expansion of tissue after formalin fixation > (around 10%), but then certainly a shrinkage to 100% dehydration agent of > about 20% from the original size. We found similar results with alcohol, > acetone and THF. > > This is manual fluid changes or a day per solution, with no vacuum, temp or > pressure. > > Happy to share the data if anyone is interested > > yours, > mills > > On Mon, Feb 22, 2016 at 1:10 PM, Terri Braud via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > LOL...Shrinkage...heh, heh. > > But seriously, there should be little to no gross shrinkage from formalin > > fixation and if the specimen is properly fixed, then there should be very > > little gross shrinkage as it is dehydrated. That is supposed to be the > > point! If someone is getting 30% shrinkage, there is something seriously > > wrong with their processing schedule. > > Sincerely, Terri > > Terri L. Braud, HT(ASCP) > > Anatomic Pathology Supervisor > > > > Today's Topics: > > 2. formalin and shrinkage (Gudrun Lang) > > > > > > Message: 2 > > Date: Mon, 22 Feb 2016 16:59:21 +0100 > > From: "Gudrun Lang" > > Subject: [Histonet] formalin and shrinkage > > Hi! > > Today someone asked me about shrinkage caused by the fixation with > > formaldehyde specially on skin-biopsies. She spoke about shrinkage of > 30% > > percent. In my opinion shrinkage is mainly caused by the processing with > > dehydration and defatting. Formaldehyde renders the tissue harder but not > > strictly smaller. > > What is the opinion of the community? > > Gudrun > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Caroline Miller (mills) > Director of Histology > 3Scan.com > 415 2187297 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Stacy_McLaughlin at cooley-dickinson.org Tue Feb 23 08:42:25 2016 From: Stacy_McLaughlin at cooley-dickinson.org (Stacy McLaughlin) Date: Tue, 23 Feb 2016 14:42:25 +0000 Subject: [Histonet] H.Pylori Message-ID: Hello in Histoland! We use Cell Marque's polyclonal H.Pylori on our Ventana Benchmark Ultra with Ultraview DAB detection. We're having issues with a precipitate appearing on our slides. Has anyone else experienced this and has anyone had success in resolving the issue? Thank you for your answers. Stacy Stacy McLaughlin, HT (ASCP) Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton, MA 01060 From criley at dpspa.com Tue Feb 23 10:20:41 2016 From: criley at dpspa.com (Charles Riley) Date: Tue, 23 Feb 2016 11:20:41 -0500 Subject: [Histonet] Giemsa Tissue control options Message-ID: I need to run a giemsa test and haven't done it in a long time. Is there something I can use as a control tissue other than a blood smear or spleen? -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE From wbenton at cua.md Tue Feb 23 10:40:30 2016 From: wbenton at cua.md (Walter Benton) Date: Tue, 23 Feb 2016 16:40:30 +0000 Subject: [Histonet] Giemsa Tissue control options In-Reply-To: References: Message-ID: http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/MGIEMSA.PDF -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, February 23, 2016 11:21 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Giemsa Tissue control options I need to run a giemsa test and haven't done it in a long time. Is there something I can use as a control tissue other than a blood smear or spleen? -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From doolee at shands.ufl.edu Tue Feb 23 11:36:43 2016 From: doolee at shands.ufl.edu (Dooley, Elaine) Date: Tue, 23 Feb 2016 17:36:43 +0000 Subject: [Histonet] h. pylori Message-ID: <6193C53146742E4586DD4838F47F79AE0EA29B99@MSXMB02.Shands.local> Hi, We filter our H.Pylori antibody from Cell Marque before using it on our Ventana Ultra and XTs and this has made it a cleaner stain. Elaine Dooley Shands Teaching Hospital Gainesville FL 352-265-0111 ext 72117 From doolee at shands.ufl.edu Tue Feb 23 11:45:42 2016 From: doolee at shands.ufl.edu (Dooley, Elaine) Date: Tue, 23 Feb 2016 17:45:42 +0000 Subject: [Histonet] CD24 Message-ID: <6193C53146742E4586DD4838F47F79AE0EA29BB3@MSXMB02.Shands.local> Hi Histonetters, I have been trying to get CD24(SN3b)( cat# MA5-11833) from Thermo Fisher to stain paraffin sections with no success. I tried antigen retrieval with citra and cc1. I have tried ovarian tumor tissue, tonsil, colon tumor tissue and did not get any staining. So I called Thermo Fisher and they said to try the antibody without antigen retrieval and I did that and still did not get any staining. So I tried protease 2 digestion for 8 min. and still did not get any staining. I have been trying the antibody at a 1:50 dilution for 1 to 2 hours. Does anyone have any suggestions on how to make this antibody work? Elaine Dooley U of FL 352-265-0111 ext 72117 From CDavis at che-east.org Tue Feb 23 12:11:51 2016 From: CDavis at che-east.org (Cassie P. Davis) Date: Tue, 23 Feb 2016 18:11:51 +0000 Subject: [Histonet] H & E Troubleshooting Message-ID: <5C815EADE724D14AA0CC8F037C4185F033359B3C@SB01MSTMBX13.sb.trinity-health.org> Hi Histo folks, We are having a fun time troubleshooting our H&E because the problem in not consistent... Yesterday we poured fresh xylene, Hematoxylin , Eosin & bumped our alcohol since our ordered had not arrived yet. One of our doctor said our hematoxylin & eosin was readable but too light(he got 6 out of the 36 cases), the other (who got the 30 cases)said it was good. We had half the number of slides going through staining than we normally do. We don't let the last alcohol before xylene get pink from eosin. Had it been a Thursday-Friday I would be concerned the xylene needed to be changed. The Define and Bluing were made fresh. My theory is the tap water could be off or the xylene needs to be changed. Another theory floating in the lab is because we bleach the stain buckets over the weekend maybe the bleach is saturating the bucket and slowly leaching out diluting the hematoxylin later in the day. (I would think this would be the case for all the slides not just the last few) Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From mjones at metropath.com Tue Feb 23 12:46:01 2016 From: mjones at metropath.com (Michael Ann Jones) Date: Tue, 23 Feb 2016 18:46:01 +0000 Subject: [Histonet] H & E Troubleshooting Message-ID: Watch your soap residue in the staining dishes. That will mess you up. Bleach does leave a residue, that?s why we then wash in soap. But if you wash in soap you must use a soap residue measurement to prove the soap is rinsed clean. It will change the pH of your Heme. Spent a long 6 months figuring that out!! Such a subtle influence. Good luck! Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com On 2/23/16, 11:11 AM, "Cassie P. Davis via Histonet" wrote: >Hi Histo folks, > > We are having a fun time troubleshooting our H&E because the >problem in not consistent... > > > >Yesterday we poured fresh xylene, Hematoxylin , Eosin & bumped our >alcohol since our ordered had not arrived yet. One of our doctor said our >hematoxylin & eosin was readable but too light(he got 6 out of the 36 >cases), the other (who got the 30 cases)said it was good. > > > >We had half the number of slides going through staining than we normally >do. > >We don't let the last alcohol before xylene get pink from eosin. > >Had it been a Thursday-Friday I would be concerned the xylene needed to >be changed. > >The Define and Bluing were made fresh. > > > >My theory is the tap water could be off or the xylene needs to be changed. > >Another theory floating in the lab is because we bleach the stain buckets >over the weekend maybe the bleach is saturating the bucket and slowly >leaching out diluting the hematoxylin later in the day. (I would think >this would be the case for all the slides not just the last few) > > >Cassandra Davis >Histology Technician >Anatomical Pathology Laboratory >Saint Francis Healthcare >701 N. Clayton Street >Wilmington,DE 19805 >Office: 302-575-8095 >Email: CDavis at che-east.org >www.saintfrancishealthcare.org > > >Confidentiality Notice: >This e-mail, including any attachments is the property of Trinity Health >and is intended for the sole use of the intended recipient(s). It may >contain information that is privileged and confidential. Any >unauthorized review, use, disclosure, or distribution is prohibited. If >you are not the intended recipient, please delete this message, and reply >to the sender regarding the error in a separate email. >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lauren.Richey at tufts.edu Tue Feb 23 12:57:46 2016 From: Lauren.Richey at tufts.edu (Richey, Lauren) Date: Tue, 23 Feb 2016 18:57:46 +0000 Subject: [Histonet] formalin and shrinkage (Gudrun Lang) Message-ID: <7D36FC459047524E90E5EC68BE2A42083E9E8950@SSVMEXDAG01MB03.tufts.ad.tufts.edu> Gudrun, I found 3 papers that investigated tissue size after formalin fixation. Apparently, fatty cutaneous tissue may shrink, while other tissue, such as skeletal muscle, may expand. Also, some tissues (skin) may shrink after excision even prior to formalin fixation. http://www.ncbi.nlm.nih.gov/pubmed/20357519 Oncology. 2010;78(2):115-24. doi: 10.1159/000306140. Epub 2010 Mar 31. Formalin fixation could interfere with the clinical assessment of the tumor-free margin in tumor surgery: magnetic resonance imaging-based study. Docquier PL1, Paul L, Cartiaux O, Lecouvet F, Dufrane D, Delloye C, Galant C. http://www.dermpathmd.com/skin_tags/Shrinkage%20of%20Cutaneous%20Specimens%20.pdf J Cutan Pathol 2008: 35: 1093-1096 Shrinkage of cutaneous specimens: formalin or other factors involved? https://www.dovepress.com/breast-specimen-shrinkage-following-formalin-fixation-peer-reviewed-fulltext-article-PLMI Pathology and Laboratory Medicine International 2014; 6: 11-14 Breast specimen shrinkage following formalin fixation This was interesting to me because I have (apparently incorrectly) always thought tissues expanded somewhat in formalin since cut surfaces tend to bulge and it is sometimes harder to get large samples out of narrow-mouth containers after fixation than to put them in. Lauren Lauren Richey, DVM, PhD, Diplomate ACVP Veterinary Pathologist Director, Tufts Comparative Pathology Services Assistant Director, DLAM Tufts University Boston MA 02111 617-636-6488 http://sites.tufts.edu/histopath Today's Topics: 1. Re: formalin and shrinkage (Jay Lundgren) 2. Re: formalin and shrinkage (Michael Ann Jones) 3. Re: Davidson's fixative and IHC question (Jay Lundgren) 4. Re: formalin and shrinkage (Gudrun Lang) 5. Re: Davidson's fixative and IHC question (Gudrun Lang) 6. Re: Shrinkage (Terri Braud) 7. BCL-10 for IHC-P (Bitting, Angela K.) 8. Re: Shrinkage (Caroline Miller) 9. Re: Shrinkage (Colleen Forster) 10. H.Pylori (Stacy McLaughlin) 11. Giemsa Tissue control options (Charles Riley) 12. Re: Giemsa Tissue control options (Walter Benton) 13. h. pylori (Dooley, Elaine) 14. CD24 (Dooley, Elaine) ---------------------------------------------------------------------- Message: 1 Date: Mon, 22 Feb 2016 13:30:46 -0600 From: Jay Lundgren To: gu.lang at gmx.at Cc: histonet Subject: Re: [Histonet] formalin and shrinkage Message-ID: Content-Type: text/plain; charset=UTF-8 I was taught at AFIP to expect shrinkage of 10%, in each dimension. So I guess that's 30% shrinkage overall? Shrinkage is partially caused by formalin crosslinking the proteins in fixation, and partially by dehydration. Maybe a little shrinkage in xylene too? From removal of fat in adipose tissues? http://link.springer.com/article/10.1007/BF00695061#page-1 Is your Pathologist really concerned about shrinkage, or about curling and distortion of small shave bxs? Because a certain degree of shrinkage is an unavoidable artifact of tissue processing. If it's the latter, I like to use 2 blue sponges. I find they really help to keep things flat and oriented. Some people don't like them because of carryover. I just say change your processor reagents more often. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Feb 22, 2016 at 9:59 AM, Gudrun Lang via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi! > > Today someone asked me about shrinkage caused by the fixation with > formaldehyde specially on skin-biopsies. She spoke about shrinkage of > 30% percent. In my opinion shrinkage is mainly caused by the > processing with dehydration and defatting. Formaldehyde renders the > tissue harder but not strictly smaller. > > > > What is the opinion of the community? > > > > Gudrun > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Lacie.Algeo at providence.org Tue Feb 23 13:25:28 2016 From: Lacie.Algeo at providence.org (Algeo, Lacie A) Date: Tue, 23 Feb 2016 19:25:28 +0000 Subject: [Histonet] staffing Message-ID: <24C4B3C167E5694887AB594C7602CE3A03C777BF@WN35104.or.providence.org> Hi All, I am trying to collect a little more data to justify an increase in technical staffing in my facility. Would anyone be willing/able to provide? Average blocks per day Total technical FTE Average IHC slides per day Average FS per day Thank you so much! Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo at providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From LRaff at uropartners.com Tue Feb 23 13:46:11 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 23 Feb 2016 19:46:11 +0000 Subject: [Histonet] Staffing Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B6EC33E@COLOEXCH01.uropartners.local> Average per day: 150 blocks with 6 levels on 2 slides for every block About 20-25 IHC slices About 22 technical hours NO FS, but histo techs are also involved in specimen accessioning. ______________________________ Music trivia(l) question: http://www.chicagonow.com/downsize-maybe/2016/02/music-trivia-tuesday-what-do-neil-young-and-neil-diamond-have-in-common/ Other recent post: http://www.chicagonow.com/downsize-maybe/2016/02/would-we-still-want-to-be-on-family-feud/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From kristyn.ferber at gmail.com Tue Feb 23 15:09:03 2016 From: kristyn.ferber at gmail.com (Kristyn Ferber) Date: Tue, 23 Feb 2016 16:09:03 -0500 Subject: [Histonet] Giemsa Tissue control options Message-ID: Charles, If you are looking for microorganisms you can try colon. Kristyn From rsrichmond at gmail.com Tue Feb 23 15:22:50 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Tue, 23 Feb 2016 16:22:50 -0500 Subject: [Histonet] Davidson's fixative and IHC question Message-ID: Several queries about the possible effect of Davidson's fixative on immunohistochemistry. I have a lot of experience with Davidson's fixative, but none with its effect on IHC. Davidson's fixative is easily prepared: 3 parts tap water 3 parts ethanol or reagent alcohol 2 parts 37% formalin (not neutral buffered formalin) 1 part glacial acetic acid It is quite destructive to H & E staining if fixation is at all prolonged. Tissue should be taken out of it in not more much more than 24 hours. The history of Davidson's fixative is somewhat obscure - I have a good bit of information on it. John Kiernan has said he'll work on ascertaining more about its history if he ever has the time. Bob Richmond Samurai Pathologist Maryville TN From jsjurczak at comcast.net Tue Feb 23 15:45:30 2016 From: jsjurczak at comcast.net (jsjurczak at comcast.net) Date: Tue, 23 Feb 2016 21:45:30 +0000 (UTC) Subject: [Histonet] Davidson's fixative and IHC question In-Reply-To: References: Message-ID: <2022364858.4635139.1456263930872.JavaMail.zimbra@comcast.net> Is this a good fixative for fetal brain? Anybody have any suggestions as to protocols? Do any of the readers here do fetal (human) brain on a regular basis? ----- Original Message ----- From: "Bob Richmond via Histonet" To: "Histonet at lists.utsouthwestern.edu" Sent: Tuesday, February 23, 2016 3:22:50 PM Subject: Re: [Histonet] Davidson's fixative and IHC question Several queries about the possible effect of Davidson's fixative on immunohistochemistry. I have a lot of experience with Davidson's fixative, but none with its effect on IHC. Davidson's fixative is easily prepared: 3 parts tap water 3 parts ethanol or reagent alcohol 2 parts 37% formalin (not neutral buffered formalin) 1 part glacial acetic acid It is quite destructive to H & E staining if fixation is at all prolonged. Tissue should be taken out of it in not more much more than 24 hours. The history of Davidson's fixative is somewhat obscure - I have a good bit of information on it. John Kiernan has said he'll work on ascertaining more about its history if he ever has the time. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DEBORAH_ELLENBURG at bshsi.org Tue Feb 23 16:03:32 2016 From: DEBORAH_ELLENBURG at bshsi.org (Ellenburg, Deborah) Date: Tue, 23 Feb 2016 22:03:32 +0000 Subject: [Histonet] unscribe Message-ID: Unscribe Deborah Ellenburg, HT (ASCP) Clinical Lead St. Francis Health System One St. Francis Drive Greenville, SC 29601 Office Phone: 864-255-1582 Cell Phone: 864-444-8291 FAX (864) 679-8963 "This communication may contain CONFIDENTIAL and PRIVILEGED information for the sole use of the intended recipient(s). If you are not the intended recipient (or authorized to receive for the recipient), please contact the sender by reply e-mail or telephone (864-255-1670) and delete all copies of this message." ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From hhawkins at UTMB.EDU Tue Feb 23 17:11:32 2016 From: hhawkins at UTMB.EDU (Hawkins, Hal K.) Date: Tue, 23 Feb 2016 23:11:32 +0000 Subject: [Histonet] Davidson's fixative and IHC question In-Reply-To: <2022364858.4635139.1456263930872.JavaMail.zimbra@comcast.net> References: , <2022364858.4635139.1456263930872.JavaMail.zimbra@comcast.net> Message-ID: <22624908330375439D6382C9F95093FF9453A4F1@GRMBX1.utmb.edu> We briefly experimented with using a fixative for stillborn fetal brains that was a mixture of equal volumes of 20% formalin and 95% ethanol. The gross preservation and firmness of these very soft brains was improved, and the histology results were fine, but as one might predict, some antigens were not preserved for immunohistochemistry. ________________________________________ From: Jeff Jurczak via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, February 23, 2016 3:45 PM To: Bob Richmond Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Davidson's fixative and IHC question Is this a good fixative for fetal brain? Anybody have any suggestions as to protocols? Do any of the readers here do fetal (human) brain on a regular basis? ----- Original Message ----- From: "Bob Richmond via Histonet" To: "Histonet at lists.utsouthwestern.edu" Sent: Tuesday, February 23, 2016 3:22:50 PM Subject: Re: [Histonet] Davidson's fixative and IHC question Several queries about the possible effect of Davidson's fixative on immunohistochemistry. I have a lot of experience with Davidson's fixative, but none with its effect on IHC. Davidson's fixative is easily prepared: 3 parts tap water 3 parts ethanol or reagent alcohol 2 parts 37% formalin (not neutral buffered formalin) 1 part glacial acetic acid It is quite destructive to H & E staining if fixation is at all prolonged. Tissue should be taken out of it in not more much more than 24 hours. The history of Davidson's fixative is somewhat obscure - I have a good bit of information on it. John Kiernan has said he'll work on ascertaining more about its history if he ever has the time. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robert.h.byrd at gmail.com Tue Feb 23 19:30:17 2016 From: robert.h.byrd at gmail.com (Robert Byrd) Date: Tue, 23 Feb 2016 19:30:17 -0600 Subject: [Histonet] Davidson's fixative and IHC question In-Reply-To: <22624908330375439D6382C9F95093FF9453A4F1@GRMBX1.utmb.edu> References: <2022364858.4635139.1456263930872.JavaMail.zimbra@comcast.net> <22624908330375439D6382C9F95093FF9453A4F1@GRMBX1.utmb.edu> Message-ID: Bouin's solution is an excellent fixative for fetal brains, we were still using it routinely when I trained 10 years ago but has (and already had in most places) largely fallen out of use due to the explosive risk associated with dried picric acid residue in drainpipes etc. It does work remarkably well on soft and macerated fetal brains though. I use 10% NBF now out of necessity but might be able to track down a Bouin's protocol if you are interested. On Tue, Feb 23, 2016 at 5:11 PM, Hawkins, Hal K. via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > We briefly experimented with using a fixative for stillborn fetal brains > that was a mixture of equal volumes of 20% formalin and 95% ethanol. The > gross preservation and firmness of these very soft brains was improved, and > the histology results were fine, but as one might predict, some antigens > were not preserved for immunohistochemistry. > > ________________________________________ > From: Jeff Jurczak via Histonet [histonet at lists.utsouthwestern.edu] > Sent: Tuesday, February 23, 2016 3:45 PM > To: Bob Richmond > Cc: Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Davidson's fixative and IHC question > > Is this a good fixative for fetal brain? Anybody have any suggestions as > to protocols? Do any of the readers here do fetal (human) brain on a > regular basis? > > ----- Original Message ----- > > From: "Bob Richmond via Histonet" > To: "Histonet at lists.utsouthwestern.edu" > > Sent: Tuesday, February 23, 2016 3:22:50 PM > Subject: Re: [Histonet] Davidson's fixative and IHC question > > Several queries about the possible effect of Davidson's fixative on > immunohistochemistry. > > I have a lot of experience with Davidson's fixative, but none with its > effect on IHC. > > Davidson's fixative is easily prepared: > > 3 parts tap water > 3 parts ethanol or reagent alcohol > 2 parts 37% formalin (not neutral buffered formalin) > 1 part glacial acetic acid > > It is quite destructive to H & E staining if fixation is at all prolonged. > Tissue should be taken out of it in not more much more than 24 hours. > > The history of Davidson's fixative is somewhat obscure - I have a good bit > of information on it. John Kiernan has said he'll work on ascertaining more > about its history if he ever has the time. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ccrowder25 at gmail.com Tue Feb 23 21:14:07 2016 From: ccrowder25 at gmail.com (Cheryl Crowder) Date: Tue, 23 Feb 2016 21:14:07 -0600 Subject: [Histonet] Giemsa control Message-ID: Charles - The best control for a non-blood Giemsa stain is a mast cell tumor from a canine. You can probably get a block from the Univ of Penn Vet School. It is really close to you. The mast cells will stain purple or magenta. Cheryl From classicdoc at gmail.com Tue Feb 23 21:59:59 2016 From: classicdoc at gmail.com (Douglas Gregg) Date: Tue, 23 Feb 2016 22:59:59 -0500 Subject: [Histonet] Science fair student help Message-ID: Hello, I have not been on this forum for some time, but still follow it. I am a retired veterinary pathologist. Last year I posted an idea for a freshman in high school who wants to do a serious science fair project. He is already beyond high school science level. His father found me because I have been a science fair judge for many years at a nearby school district. At first, I declined, citing that I was retired and did not have a good project for him. Later I thought about a recent study I read about honey bees being infected with an Iridovirus. That caught my attention because I did my dissertation and worked for about 10 years on African swine fever (ASF) caused by an Iridovirus (now renamed but only moved to a branch classification by itself). It is the only mammalian Iridovirus disease. Through mass spectrograph studies, and subtraction analysis of normal bee data from collapsed colony bees, the US Army found the fingerprint of a likely Iridovirus infection in colony collapse syndrome. No one has yet confirmed this by other methods that are more conventional and it has not been fully accepted due to the new technology used to find it. So I suggested to Joe, that he could attempt to identify the virus in bee larva tissues using histology to find inclusions and immunostaining using Vector staining system. I used immunostaining through most of my career and was one of the first to identify a virus infection (ASF) with Vector staining systems back in the early 80s. I have a microtome and a few paraffin mold trays and a few plastic paraffin tissue holders left over from a consulting project 6 years ago. What he will need is some paraplast, more plastic molds, a few metal trays, Harris hematoxylin, eosin, slides, coverslips, permount and later PBS, Tris and a Vector ABC AP kit which I am very hopeful can be donated by Vector when we get that far. I know histo labs often have lots of old unused supplies around that don't necessarily fit into current routines or machines. If any of you have such supplies that could be used for very manual processing of tissues, they would be greatly appreciated. A warm water bath is needed too but we can improvise, if necessary. Having reviewed the literature on honeybee colony collapse syndrome and comparing the pathogenesis with African swine fever, an Iridovirus infection of bees is a very good fit. I think there is a very high likelihood that this could be a big breakthrough in the honey bee collapse problem that thus far has not been answered with the many hypotheses suggested. This is a worldwide problem that threatens the world food supply and must be understood and controlled soon. As a onetime bee keeper, it is close to my heart. I hope some of you can help Joe get into histology. He is very eager and this could possibly lead to a scholarship or at least a workstudy position during college in a histolab someday. Due to the recession, his family can't support this project. He has gotten some small monetary support from a local bee keeper as well as help acquiring bee larva from colonies. I am giving him space to set up a lab in my basement and the necessary solvents, etc and lots of training. This is a very ambitious project but I think it can be accomplished. He has 2 or 3 years to get it finished, and hopefully published. Any help would be appreciated. Any ideas for crowdsource funding would also be appreciated. Douglas Gregg DVM, PhD Retired - Plum Island Animal Disease Center Southold, NY 11971 classicdoc at gmail.com From j.benavides at eae.csic.es Wed Feb 24 03:37:53 2016 From: j.benavides at eae.csic.es (Julio Benavides) Date: Wed, 24 Feb 2016 10:37:53 +0100 Subject: [Histonet] Davidson's fixative and IHC question In-Reply-To: References: <2022364858.4635139.1456263930872.JavaMail.zimbra@comcast.net> <22624908330375439D6382C9F95093FF9453A4F1@GRMBX1.utmb.edu> Message-ID: <20160224103753.Horde.-CjKqX3fxxbkFAIh1wjcQA2@webmail.csic.es> Hi, in my experience, Bouin?s fixative can sometimes give you problems with IHC. Both because it seriously interfere with some antigens (in my case, I was doing IHC for lentivirus and herpesvirus and more than 24h of fixation would drastically reduce IHC labelling) but also because it gives you non-specific labelling (in may case, ovine neutrophils came up positive with that lentivurs Ac). Julio Robert Byrd via Histonet escribi?: > Bouin's solution is an excellent fixative for fetal brains, we were still > using it routinely when I trained 10 years ago but has (and already had in > most places) largely fallen out of use due to the explosive risk associated > with dried picric acid residue in drainpipes etc. It does work remarkably > well on soft and macerated fetal brains though. I use 10% NBF now out of > necessity but might be able to track down a Bouin's protocol if you are > interested. > > On Tue, Feb 23, 2016 at 5:11 PM, Hawkins, Hal K. via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> >> We briefly experimented with using a fixative for stillborn fetal brains >> that was a mixture of equal volumes of 20% formalin and 95% ethanol. The >> gross preservation and firmness of these very soft brains was improved, and >> the histology results were fine, but as one might predict, some antigens >> were not preserved for immunohistochemistry. >> >> ________________________________________ >> From: Jeff Jurczak via Histonet [histonet at lists.utsouthwestern.edu] >> Sent: Tuesday, February 23, 2016 3:45 PM >> To: Bob Richmond >> Cc: Histonet at lists.utsouthwestern.edu >> Subject: Re: [Histonet] Davidson's fixative and IHC question >> >> Is this a good fixative for fetal brain? Anybody have any suggestions as >> to protocols? Do any of the readers here do fetal (human) brain on a >> regular basis? >> >> ----- Original Message ----- >> >> From: "Bob Richmond via Histonet" >> To: "Histonet at lists.utsouthwestern.edu" > > >> Sent: Tuesday, February 23, 2016 3:22:50 PM >> Subject: Re: [Histonet] Davidson's fixative and IHC question >> >> Several queries about the possible effect of Davidson's fixative on >> immunohistochemistry. >> >> I have a lot of experience with Davidson's fixative, but none with its >> effect on IHC. >> >> Davidson's fixative is easily prepared: >> >> 3 parts tap water >> 3 parts ethanol or reagent alcohol >> 2 parts 37% formalin (not neutral buffered formalin) >> 1 part glacial acetic acid >> >> It is quite destructive to H & E staining if fixation is at all prolonged. >> Tissue should be taken out of it in not more much more than 24 hours. >> >> The history of Davidson's fixative is somewhat obscure - I have a good bit >> of information on it. John Kiernan has said he'll work on ascertaining more >> about its history if he ever has the time. >> >> Bob Richmond >> Samurai Pathologist >> Maryville TN >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.benavides at eae.csic.es Wed Feb 24 03:55:33 2016 From: j.benavides at eae.csic.es (Julio Benavides) Date: Wed, 24 Feb 2016 10:55:33 +0100 Subject: [Histonet] Collodian bag for cell blocks In-Reply-To: References: Message-ID: <20160224105533.Horde.3_fS9G0sgwy9jAjPQiVjjQ1@webmail.csic.es> Hi, I have a question regarding the use of UCSF Collodion Bag Cell blocks. once you do the bag, you pour in the cells in, let?s say 10ml of formalin. Then, how do you proceed? Do you spin down the cells with the bag inside a plastic tube? do you take then put the formalin and cut the top of the bag into a cassette? do you close the bag with a thread with the cells and formalin?.... Any help with this would be greatly appreciated, Thanks a lot julio WILLIAM DESALVO via Histonet escribi?: > What method are you using? Making your own coated tubes, UCSF or MD > Anderson. Are you purchasing coated tubes? Once the tube is coated, > dried (using hood) and filled w dH2O, there should be no odor. > Always work w/ collodion under the hood. Ethyl ether is main > component. Once in cassette and processed, there should not be odor. > > Sent from my Windows Phone > ________________________________ > From: Baldwin, Kathy via Histonet > Sent: ?1/?4/?2016 2:07 PM > To: > 'histonet at lists.utsouthwestern.edu' > Subject: [Histonet] Collodian bag for cell blocks > > Hi All > Was wondering if anyone is using the Collodian bag for cell blocks. > We just got it and have been using it 'works great' but all the > techs are complaining of the smell.. Our ventilation has been looked > at and has passed however the smell still lingers in the room. Any > suggestions?? > > > Thanks > S. Kathy Baldwin > Histology/Cytology Supervisor > Memorial Hospital and Health Care Center > 800 West 9th St. > Jasper, Indiana 47546 > Office 812-996-0210 > Fax 812-996-0232 > Cell 812-887-3357 > > > > > > CONFIDENTIALITY NOTICE: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and > may contain confidential and privileged information or otherwise > protected by law. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, > please contact the sender by reply e-mail and destroy all copies of > the original message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From catherinesimonson at gmail.com Wed Feb 24 05:10:15 2016 From: catherinesimonson at gmail.com (Catherine Simonson) Date: Wed, 24 Feb 2016 06:10:15 -0500 Subject: [Histonet] Issues with tissues while doing IHC/IF Message-ID: Greetings and Salutations oh wise and wonderful histology people. I am trying to work up several antibodies for IF on 10 micron thick mouse brains. These tissues were perfused and fixed in 4% PFA prior to my receiving them. I treated them with sucrose prior to freezing. The issue I am having is that the tissue is falling off the slides during staining. Using charged slides and performing (attempting to, anyhow) on the Ventana Discovery. Any suggestions would be most helpful. Thanks in advance. From jqb7 at cdc.gov Wed Feb 24 07:35:45 2016 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Wed, 24 Feb 2016 13:35:45 +0000 Subject: [Histonet] Question re: accessory piece for tissue flotation bath Message-ID: <3B2CD438E1628A41BD687E98B963B7815406B3B0@EMBX-CLFT4.cdc.gov> Morning! Can anyone of you share the functionality of: Flotation Work Station w/ 8" x 8" x 2 1/4" (Deep Dish), HistoOrientator and Dryer Curious if the HistoOrientator actually removes wrinkles as the description states. Thanks! Jeanine H. Sanders, BS, HT (ASCP), QIHC Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS/G-32 Atlanta, GA 30329 jqb7 at cdc.gov 404-639-3590 From bakevictoria at gmail.com Wed Feb 24 08:18:05 2016 From: bakevictoria at gmail.com (Victoria Baker) Date: Wed, 24 Feb 2016 09:18:05 -0500 Subject: [Histonet] Thromboplastin for cell blocks Message-ID: Hi I am looking for Thromboplastin to make cell blocks. I can't remember who we got it from years ago. Any information is much appreciated. Vikki From CDavis at che-east.org Wed Feb 24 08:21:12 2016 From: CDavis at che-east.org (Cassie P. Davis) Date: Wed, 24 Feb 2016 14:21:12 +0000 Subject: [Histonet] H & E Troubleshooting In-Reply-To: <38667E7FB77ECD4E91BFAEB8D9863863388F32A587@LRGHEXVS1.practice.lrgh.org> References: <5C815EADE724D14AA0CC8F037C4185F033359B3C@SB01MSTMBX13.sb.trinity-health.org>, <38667E7FB77ECD4E91BFAEB8D9863863388F32A587@LRGHEXVS1.practice.lrgh.org> Message-ID: <5C815EADE724D14AA0CC8F037C4185F033359B98@SB01MSTMBX13.sb.trinity-health.org> "Another theory floating in the lab is because we bleach the stain buckets over the weekend maybe the bleach is saturating the bucket and slowly leaching out diluting the hematoxylin later in the day. (I would think this would be the case for all the slides not just the last few)" I guess I need to give more details...the first few runs look "fine" per my supervisor. If we didn't rinse the buckets I would think the first few runs would look pale too...Leaching out bleach later in the day seems illogical to me. This is why I am leaning toward the xylene being saturated (but we didn't have half the cases that we normally do and it was fresh Monday morning when this occurred) Or the tap water which is hooked directly to our stainer and can vary from clean to yellow to tan within a day. I had this issue with the tap water when I was at another facility a few block from this one in the same city. I just need another voice of reason to show my supervisor. Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org ________________________________________ From: Podawiltz, Thomas [tpodawiltz at lrgh.org] Sent: Tuesday, February 23, 2016 1:28 PM To: Cassie P. Davis Subject: RE: H & E Troubleshooting Hi Cassandra, Does the Eosin looked washed out. If so it may be your bluing. I had this issue a year or so ago and it turned out that the bluing was to strong and it was effecting the ph of the Eosin and giving it a washed out look. Since I had a gallon of the stuff, I just adjusted the time in bluing and my water rinse and the issue went away. I will say I have also had the issue with bleached containers, but when that happened everything look light. Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare 603-524-3211 ext: 3220 -----Original Message----- From: Cassie P. Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, February 23, 2016 1:12 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] H & E Troubleshooting Hi Histo folks, We are having a fun time troubleshooting our H&E because the problem in not consistent... Yesterday we poured fresh xylene, Hematoxylin , Eosin & bumped our alcohol since our ordered had not arrived yet. One of our doctor said our hematoxylin & eosin was readable but too light(he got 6 out of the 36 cases), the other (who got the 30 cases)said it was good. We had half the number of slides going through staining than we normally do. We don't let the last alcohol before xylene get pink from eosin. Had it been a Thursday-Friday I would be concerned the xylene needed to be changed. The Define and Bluing were made fresh. My theory is the tap water could be off or the xylene needs to be changed. Another theory floating in the lab is because we bleach the stain buckets over the weekend maybe the bleach is saturating the bucket and slowly leaching out diluting the hematoxylin later in the day. (I would think this would be the case for all the slides not just the last few) Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From philip_manfre at merck.com Wed Feb 24 08:50:59 2016 From: philip_manfre at merck.com (Manfre, Philip) Date: Wed, 24 Feb 2016 09:50:59 -0500 Subject: [Histonet] Question re: accessory piece for tissue flotation bath In-Reply-To: <3B2CD438E1628A41BD687E98B963B7815406B3B0@EMBX-CLFT4.cdc.gov> References: <3B2CD438E1628A41BD687E98B963B7815406B3B0@EMBX-CLFT4.cdc.gov> Message-ID: <558A4571351D0C42BD923F403F4198C40109FE338AC7@USCTMXP51014.merck.com> With regards to the HistoOrientator, it does removes wrinkles from the sections fairly effectively. That being said, I would not use it on any sections intended for immunohistochemistry. The hot plate on this device gets very hot and would no doubt damage or affect the antigenicity of such tissues. Also, some practice is required to effectively use it. If you do not drain the slides somewhat first, the tissues will quickly move all over the slide as they heat. They will move quite a bit, sometimes butting up against each other. If you drain them too long, then the tissues start to dry onto the slide, wrinkles and all. So, as I said, spend some time practicing with this device with practice tissue until you get the hang of it. It is a good tool that is tricky to use at first. Good luck! Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre at merck.com -----Original Message----- From: Sanders, Jeanine (CDC/OID/NCEZID) via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, February 24, 2016 8:36 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Question re: accessory piece for tissue flotation bath Morning! Can anyone of you share the functionality of: Flotation Work Station w/ 8" x 8" x 2 1/4" (Deep Dish), HistoOrientator and Dryer Curious if the HistoOrientator actually removes wrinkles as the description states. Thanks! Jeanine H. Sanders, BS, HT (ASCP), QIHC Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS/G-32 Atlanta, GA 30329 jqb7 at cdc.gov 404-639-3590 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From tgenade at gmail.com Wed Feb 24 08:51:21 2016 From: tgenade at gmail.com (Tyrone Genade) Date: Wed, 24 Feb 2016 08:51:21 -0600 Subject: [Histonet] Davidson's fixative and IHC question Message-ID: Hello, I am using Davidson's fixative to fix whole fish and I am very happy with the results. I had tried 4% PFA but had issues with collapsed eyes and detached retinas... No such issues with Davidson's or Bouin's. In my experience I get sharper nuclear staining by H&E and better declacification with Davidson's. The integrity of the brain tissues is good with less shrinkage than with Bouin's. I fix whole fish over night and then move them to 70% ethanol. I had issues with autofluoresence with Davidson's (no surprise there) but it was easily remedied using Gayle Callis' glycine protocol (available somewhere on histonet). Same story for Bouin's. Some antigens are lost and seem irretrievable. The anti-GFAP antibody, GA5*, worked fine on PFA fixed fish but the epitope couldn't be retrieved in Bouin's fixed fish. I'm not sure about Davidson's but using heat induced citric acid antigen retrieval (pH 5, 80 min at 60 oC) has worked for several epitopes. * If memory serves, this is a phosphate-epitope so that could be something to look out for. I would advise testing several different fixatives and seeing which works the best for your application. If you are working with soft tissue, such as brain, a zinc fixative might be better for the preservation of delicate epitopes: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1919503/ . Bye Tyrone From LBUSTAMANTE at cvm.tamu.edu Wed Feb 24 09:58:36 2016 From: LBUSTAMANTE at cvm.tamu.edu (Bustamante, Lin) Date: Wed, 24 Feb 2016 15:58:36 +0000 Subject: [Histonet] Glyco Kit Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC9015813BB6B@CVMMB02.cvm.tamu.edu> If you do Plastic embedding from a Kit, could you please share your experience? Please mention Kit name in your response. Thank you very much. Lin. Lin S. Bustamante B.Sc. H.T.(ASCP) Research Associate Texas A&M University College of Veterinay Medicine VIBS Histology Laboratory Supervisor Room 107 VMA College Station, Texas 77843-4458 (979)845-3177 (979)458-3499 Fax From Lisa.White3 at va.gov Wed Feb 24 10:06:09 2016 From: Lisa.White3 at va.gov (White, Lisa M.) Date: Wed, 24 Feb 2016 11:06:09 -0500 Subject: [Histonet] Davidson's fixative and IHC question Message-ID: <2B2ECF33934F5D4996D8BE03EFDF39760C478A76@VHAV09MSGA3.v09.med.va.gov> We have had failure of IHC on CD-20 when fixed in Davidson's. So it is possible. The only thing to do is validate all stains you will use with the Davidson's. Lisa White From relia1 at earthlink.net Wed Feb 24 10:21:01 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 24 Feb 2016 11:21:01 -0500 Subject: [Histonet] Histotechs and Supervisors needed in Texas and Arkansas. Can you help? Message-ID: <003b01d16f1f$5c45c280$14d14780$@earthlink.net> Hi Histonetters! How are you? I hope you are having a great week! I was hoping you might be able to help me. I am presently working with a client in Dallas in need of a histotech and a client in Amarillo in need of histology supervisor. I am also working with a client in Little Rock, AR in need of histotechs and a client in Fayetteville, AR in need of a lead tech. The help I need from you is do you know anyone that might be interested in hearing about either of these opportunities? If so could you please forward my e-mail to them? These are full time day shift permanent positions. If you are interested in either of these positions please contact me ASAP toll free at 866-607-3542, cell/text 407-353-5070 or via email at relia1 at earthlink.net If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won?t release it to anyone without your permission. Thanks-Pam cell: 407-353-5070 email: relia1 at earthlink.net Right Place, Right Time, Right Move with RELIA! Thanks-Pam Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From LBUSTAMANTE at cvm.tamu.edu Wed Feb 24 11:14:53 2016 From: LBUSTAMANTE at cvm.tamu.edu (Bustamante, Lin) Date: Wed, 24 Feb 2016 17:14:53 +0000 Subject: [Histonet] FW: Advice regards NaDH and SDH stains? In-Reply-To: <56AFE8EDBBA35E4C98D45C06550227E40D02709B@CVMMB01.cvm.tamu.edu> References: <56AFE8EDBBA35E4C98D45C06550227E40D02709B@CVMMB01.cvm.tamu.edu> Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC9015813BC04@CVMMB02.cvm.tamu.edu> Is anyone out there able to help this student? We don't perform, less analyze these stains. Thank you very much. Lin. +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ NaDH and SDH stains We are planning to perform magnetic resonance spectroscopy on the cranial sartorius and vastus lateralis muscles of control dogs and dogs with Golden Retriever Muscular Dystrophy to evaluate for differences in the concentration of various metabolites and then compare this with NaDH and SDH stains of biopsy samples from the same sites. However, I am having some difficulty determining how to compare them because I do not know how these stains are normally analyzed; do you employ a grading scale based on the severity of change? I am trying to confirm which statistical analyses to apply to compare the spectroscopic findings with the histological staining characteristics and thus really need to figure out exactly how the stains will be interpreted! I have been reading the literature to see how NaDH / SDH staining characteristics correlate with muscular dystrophy but it is proving a struggle. I was hoping you might be able to recommend a reference text? Thank you so much in advance. If there is someone else you would prefer I contact about this then please do let me know. Thanks again, Sarah From liz at premierlab.com Wed Feb 24 13:10:43 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Wed, 24 Feb 2016 12:10:43 -0700 Subject: [Histonet] FW: Scholarship Offered by Digital Pathology Association In-Reply-To: <39d05c4973-liz=premierlab.com@mail.vresp.com> References: <39d05c4973-liz=premierlab.com@mail.vresp.com> Message-ID: <14E2C6176416974295479C64A11CB9AE02854EF5430B@SBS2K8.premierlab.local> FYI ? See below and while I have your attention there are lots of other Awards and Scholarships that NSH offers its members. So nominate yourself or one of your colleagues. It easy to do, all the information is at the link below. http://www.nsh.org/content/nsh-awards-and-scholarships Liz NSH Awards Chair Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Digital Pathology Association [mailto:Digital_Pathology_Association at mail.vresp.com] Sent: Wednesday, February 24, 2016 12:03 PM To: Elizabeth Chlipala Subject: Scholarship Offered by Digital Pathology Association [http://img-ak.verticalresponse.com/social_sharing/social_sharing.placeholder.facebook.png] [http://img-ak.verticalresponse.com/social_sharing/social_sharing.placeholder.twitter.png] [http://img-ak.verticalresponse.com/social_sharing/social_sharing.placeholder.linkedin.png] [http://img-ak.verticalresponse.com/media/7/8/b/78b7da6225/39d05c4973/87ffbd58a0/library/website-header%204.jpg] Scholarship Offered by Digital Pathology Association The DPA, offers a scholarship intended to support the advancement of knowledge and continuing education in digital pathology. It is presented to a National Society for Histotechnology (NSH) member certified in histology, and currently utilizing Whole Slide Imaging (WSI) in the clinical or research laboratory. The amount of the scholarship is $1,500 and is to be used for the purpose of attending Pathology Visions, the annual meeting of the DPA. Pathology Visions provides attendees the opportunity to learn about real-world, practical applications in the ever-evolving field of digital pathology through workshops and presentations which are separated into three tracks ? Education/Research, Clinical, and Image Analysis. Attendees at Pathology Visions get direct access to industry leaders and the chance to see the latest product solutions. Pathology Visions 2016 is taking place October 23 - 25 in San Diego, CA. All applications must be completed and submitted to NSH by June 1, 2016. To learn more about the scholarship and apply, click here. The recipient of the scholarship will be selected by NSH. About the Digital Pathology Association The Digital Pathology Association, located in Indianapolis, IN, was founded in 2009. Its mission is to facilitate education and awareness of digital pathology applications in healthcare and life sciences. Members are encouraged to share best practices and promote the use of technology among colleagues in order to demonstrate efficiencies, awareness, and its ultimate benefits to patient care. To learn more about the DPA and its members and membership opportunities, please visit https://digitalpathologyassociation.org. 10293 N. Meridian Street | Suite 175 | Indianapolis, IN 46290 | P 317.816.1630 | F 317.816.1633 | E info at digitalpathologyassociation.org ________________________________ Click to view this email in a browser If you no longer wish to receive these emails, please reply to this message with "Unsubscribe" in the subject line or simply click on the following link: Unsubscribe ________________________________ Digital Pathology Association 10293 N. Meridian St. Suite 175 Indianapolis, Indiana 46290 US Read the VerticalResponse marketing policy. [http://img-ak.verticalresponse.com/pwrby_vr_logo_120.gif] [http://cts.vresp.com/o.gif?39d05c4973/005a3a31bd/mlpftw] From tejohnson at genoptix.com Wed Feb 24 13:18:22 2016 From: tejohnson at genoptix.com (Teri Johnson) Date: Wed, 24 Feb 2016 19:18:22 +0000 Subject: [Histonet] H&E Troubleshooting Message-ID: <55683c6bf89c428299b3325683353278@PHUSCB-SP37MB03.genoptix.org> Hi Cassie, I would strongly suspect your tap water. If you have a tap water rinse before hematoxylin, the pH or treatment additives carrying over could be weakening your stain. We fixed this problem previously by adding a bucket of DI water between the tap water wash and the hematoxylin, and that fixed it for us. Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From agleiberman at buffalobiolabs.com Wed Feb 24 15:51:13 2016 From: agleiberman at buffalobiolabs.com (Anatoli Gleiberman) Date: Wed, 24 Feb 2016 21:51:13 +0000 Subject: [Histonet] Davidson's fixative and IHC question In-Reply-To: References: Message-ID: My experience with small tissue samples (such as half of mouse brain or slice of mouse liver) fixed in Davidson or other alcoholic formalin fixatives is to fix it no more than overnight and transfer into 70% ethanol (almost forever). The majority of antigens will be preserved much better than in regular NBF. Anatoli Gleiberman, PhD Director of Histopathology Buffalo Biolabs LLC, 73 High Street Buffalo, NY, 14203 Phone:716-849-6810x354 e-mail:agleiberman at buffalobiolab.com -----Original Message----- From: Jay Lundgren via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, February 22, 2016 2:53 PM To: Judi Ford Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Davidson's fixative and IHC question Today I learned what Davidson's fixative is! Thanks! Anyway, so it's a strong alcoholic formalin. The answer to your question is; yes. You need to be careful of overfixation, thus masking the epitope in question. From Dako: " Of the many pre-analytical variables which affect IHC and ISH results, fixation is probably the most significant, impacting many other variables such as antigen retrieval and epitope binding. Unfortunately, to date, no single fixative has proven to be ideal for all targets and detection methods. However, it is generally more deleterious for tissue to be ?underfixed?, rather than ?overfixed?." Make sure not to leave your specimen in Davidson's for more than 24 hrs, and then in formalin for *less* than 72 hrs and you should be OK. If staining is unsatisfactory you might have to tinker with your antigen retrieval protocol (longer) or primary Ab incubation time (longer). Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Feb 22, 2016 at 11:45 AM, Judi Ford via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Everyone. Hope you all had a really good weekend. Thanks for the > replies to my double ihc question. > > I do have another question; this one is from a friend of mine. Her > client wants to do ihc on rabbit eye tissue. The tissue will be fixed > in Davidson's fixative for 24 hours then in 10% NBF. Will this have > affect future ihc projects? > > Thanks, > Judi > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tgenade at gmail.com Wed Feb 24 16:08:00 2016 From: tgenade at gmail.com (Tyrone Genade) Date: Wed, 24 Feb 2016 16:08:00 -0600 Subject: [Histonet] metal enhanced DAB Message-ID: Hello, I'm using the Co/Ni enhanced DAB from Histological & Histochemical Methods (2nd Ed). Does the solution normally turn a blue-gray after adding the H2O2? I am getting a lot of back ground and precipitate on the sections. The pH is 7.3. I had used a similar method for Western Blotting that took 5 minutes for the solution to change color. This is a near instantaneous color change. Thanks Tyrone From eca9 at georgetown.edu Thu Feb 25 08:06:35 2016 From: eca9 at georgetown.edu (Eva Permaul) Date: Thu, 25 Feb 2016 09:06:35 -0500 Subject: [Histonet] Human nuclear antibody MAB1281 Message-ID: Good morning, We recently bought MAB1281 with the hope of being able to determine if the cells in a mouse model was human or mouse. We want to use it to stain FFPE tissues. I have tried it with Citrate per the companies recommendation. I ran it on a human tonsil but saw no staining at all. Is there anyone who has been able to get this antibody to work? And if so would you please share your protocol? Thank you, Eva From joost.bruijntjes at tno.triskelion.nl Thu Feb 25 08:11:33 2016 From: joost.bruijntjes at tno.triskelion.nl (Bruijntjes, J.P. (Joost)) Date: Thu, 25 Feb 2016 14:11:33 +0000 Subject: [Histonet] paraformaldehyde Message-ID: Hi all Can anyone tell me what the expiration time is for home-made paraformaldehyde? Joost From madeathridge at pastnashville.com Thu Feb 25 08:17:18 2016 From: madeathridge at pastnashville.com (Maryann Deathridge) Date: Thu, 25 Feb 2016 07:17:18 -0700 Subject: [Histonet] cassette printer Message-ID: <05749b767736432c80a0aae0408b4b3e@pastnashville.com> Hi Histoland- Looking for a refurbished cassette printer. Currently using the Shurmark cassette printer. Prefer several hoppers for color cassettes, ability to print on Activ-Flo cassettes and Simport cassettes with no issues. At this point if it can just print. Need something to get through till we make a decision on the complete "system" (specimen tracking) for our lab. Thanks Maryann Deathridge, BS HT (ASCP) Pathology Assoc. of St. Thomas 4220 Harding Pike, Bldg. SE, Suite 504 Nashville, TN 37205 (615) 298-4100 madeathridge at pastnashville.com From relia1 at earthlink.net Thu Feb 25 09:07:01 2016 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 25 Feb 2016 10:07:01 -0500 Subject: [Histonet] RELIA HOT JOB ALERT New Opportunities in AZ, NY, VA and TX Message-ID: <00bd01d16fde$2eacbb70$8c063250$@earthlink.net> Hi Histonetters! I hope your week is going well and you are getting ready for a fantastic weekend. My phone continues to ring OFF the hook and here are the latest exciting opportunities: Histotech - Flagstaff, AZ Immunohistochemistry Specialist - Long Island, NY Histology Manager - Amarillo, TX Lead Histotech - Norfolk, VA I also have exciting opportunities in CA, WI, KY, AR and MT All of these positions are full time and permanent and my clients offer relocation assistance and/or sign on bonuses. For more information please contact Pam Barker toll free at 866-607-3542 or cell/text 407-353-5070 or email at relia1 at earthlink.net Thanks and have a great day! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From mills at 3scan.com Thu Feb 25 09:14:51 2016 From: mills at 3scan.com (Caroline Miller) Date: Thu, 25 Feb 2016 07:14:51 -0800 Subject: [Histonet] paraformaldehyde In-Reply-To: References: Message-ID: I don't know if there is a hard and fast rule, but I keep 20% in the fridge for around 6 months or until the little bits start to form on the bottom. 4% I would never keep that long, maybe a week at most. Best if you want fresh, small volumes, all the time is to get those little 37% ampules and dilute as necessary I am very interested to hear other folks opinions too, as I am sure they will vary! yours, mills On Thu, Feb 25, 2016 at 6:11 AM, Bruijntjes, J.P. (Joost) via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all > > Can anyone tell me what the expiration time is for home-made > paraformaldehyde? > > Joost > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From srishan at mail.holyname.org Thu Feb 25 09:32:34 2016 From: srishan at mail.holyname.org (srishan at mail.holyname.org) Date: Thu, 25 Feb 2016 10:32:34 -0500 Subject: [Histonet] (no subject) Message-ID: Hi All. If anyone is doing the ER/PGR Immnunohistochemistry TMA with CAP, what have you been doing when when they grade some cores as code 27 ? If any one has any input regarding this, I would greatly appreciate it. CAP provide the slides, but one of our slides were missing some cores. Thanks Nirmala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From liz at premierlab.com Thu Feb 25 09:39:19 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Thu, 25 Feb 2016 08:39:19 -0700 Subject: [Histonet] Human nuclear antibody MAB1281 In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02854EF5431F@SBS2K8.premierlab.local> Eva We have worked with various antibodies in order to detect human cells in a rodent background. I know that this antibody has been referenced in the literature and I also have known of other individuals who have had success with it, but in our hands we have never been able to make it work on FFPE tissues but I believe it works in frozen sections. We have opted to try some different antibodies. NuMA -we researched this protein and determined that it may work -here is a snippet of what we found from a quick look into the Human Protein Atlas entry for NUMA1. Though it is functionally involved in the structural rearrangement of DNA during mitosis and apoptosis, it is a constitutively expressed structural protein. From the images on HPA (http://www.proteinatlas.org/ENSG00000137497-NUMA1/tissue/primary+data) it looks like it is almost universally expressed, with the one exception of hepatocytes. The vast majority of neurons, which are overwhelmingly post-mitotic, show fairly strong expression, so that's encouraging. We initially looked at the cell signaling antibody (our target was human cells in a rat background) but found that it does cross react with rat, its fine with mouse but this antibody is our hands was found to be sensitive to time in fixation and retrieval time and temp. We opted to try another source for this antibody - abcam. They had several different antibodies for that target. We work with their antibodies a lot and they normally do not list the sequence of the antigen so we could not check the sequence homology to other species ourselves but you can call them and they will provide that information. Here is what they provided to us - "We have not tested ab86129 for cross reactivity with rat and we have not received any researcher feedback for using the antibody with this species. The immunogen sequence has 56% identity with the rat protein, so it is unlikely that it will cross react. However we have not confirmed this experimentally and we do not know if it would show non-specific staining with rat. I do think ab86129 would be the best antibody to try since it has the lowest homology with the rat protein. The other antibodies that have not been tested with rat have 69% (ab55767) or 88% (ab84680) homology." Ab86129 worked very nicely in our hands. HLA-1 this will also work nicely but the protein is expressed in the cytoplasm and expression levels can vary but it does work nicely in some instances. So for us its dependent upon the project as to which antibody we will use but the NuMA is expressed in the nuclei so it will work quite nicely for dual staining of another protein that is expressed in the cytoplasm or cell membrane. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Eva Permaul via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 25, 2016 7:07 AM To: histonet Subject: [Histonet] Human nuclear antibody MAB1281 Good morning, We recently bought MAB1281 with the hope of being able to determine if the cells in a mouse model was human or mouse. We want to use it to stain FFPE tissues. I have tried it with Citrate per the companies recommendation. I ran it on a human tonsil but saw no staining at all. Is there anyone who has been able to get this antibody to work? And if so would you please share your protocol? Thank you, Eva _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From eca9 at georgetown.edu Thu Feb 25 10:24:43 2016 From: eca9 at georgetown.edu (Eva Permaul) Date: Thu, 25 Feb 2016 11:24:43 -0500 Subject: [Histonet] Human nuclear antibody MAB1281 In-Reply-To: <14E2C6176416974295479C64A11CB9AE02854EF5431F@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE02854EF5431F@SBS2K8.premierlab.local> Message-ID: Liz, Any chance you could find out what protocol was used by the ones that have used it? or help me find some of the literature references? Everywhere I look I just see people who have not gotten it to work asking for help. Thanks, Eva On Thu, Feb 25, 2016 at 10:39 AM, Elizabeth Chlipala wrote: > Eva > > We have worked with various antibodies in order to detect human cells in a > rodent background. I know that this antibody has been referenced in the > literature and I also have known of other individuals who have had success > with it, but in our hands we have never been able to make it work on FFPE > tissues but I believe it works in frozen sections. We have opted to try > some different antibodies. > > NuMA -we researched this protein and determined that it may work -here is > a snippet of what we found from a quick look into the Human Protein Atlas > entry for NUMA1. Though it is functionally involved in the structural > rearrangement of DNA during mitosis and apoptosis, it is a constitutively > expressed structural protein. From the images on HPA ( > http://www.proteinatlas.org/ENSG00000137497-NUMA1/tissue/primary+data) > it looks like it is almost universally expressed, with the one exception of > hepatocytes. The vast majority of neurons, which are overwhelmingly > post-mitotic, show fairly strong expression, so that's encouraging. We > initially looked at the cell signaling antibody (our target was human cells > in a rat background) but found that it does cross react with rat, its fine > with mouse but this antibody is our hands was found to be sensitive to time > in fixation and retrieval time and temp. We opted to try another source > for this antibody - abcam. They had several different antibodies for that > target. We work with their antibodies a lot and they normally do not list > the sequence of the antigen so we could not check the sequence homology to > other species ourselves but you can call them and they will provide that > information. Here is what they provided to us - "We have not tested > ab86129 for cross reactivity with rat and we have not received any > researcher feedback for using the antibody with this species. The immunogen > sequence has 56% identity with the rat protein, so it is unlikely that it > will cross react. However we have not confirmed this experimentally and we > do not know if it would show non-specific staining with rat. I do think > ab86129 would be the best antibody to try since it has the lowest homology > with the rat protein. The other antibodies that have not been tested with > rat have 69% (ab55767) or 88% (ab84680) homology." Ab86129 worked very > nicely in our hands. > > HLA-1 this will also work nicely but the protein is expressed in the > cytoplasm and expression levels can vary but it does work nicely in some > instances. > > So for us its dependent upon the project as to which antibody we will use > but the NuMA is expressed in the nuclei so it will work quite nicely for > dual staining of another protein that is expressed in the cytoplasm or cell > membrane. > > Good Luck > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz at premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: Eva Permaul via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, February 25, 2016 7:07 AM > To: histonet > Subject: [Histonet] Human nuclear antibody MAB1281 > > Good morning, > We recently bought MAB1281 with the hope of being able to determine if the > cells in a mouse model was human or mouse. > We want to use it to stain FFPE tissues. I have tried it with Citrate per > the companies recommendation. I ran it on a human tonsil but saw no > staining at all. > Is there anyone who has been able to get this antibody to work? And if so > would you please share your protocol? > Thank you, > Eva > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From k84as at yahoo.com Thu Feb 25 11:00:57 2016 From: k84as at yahoo.com (mohamed abd el razik) Date: Thu, 25 Feb 2016 17:00:57 +0000 (UTC) Subject: [Histonet] Rabbit and chicken eye processing References: <1135097406.9513120.1456419657973.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1135097406.9513120.1456419657973.JavaMail.yahoo@mail.yahoo.com> Dear all What is your optimal protocol for processing rabbit and chicken eyes ? and do you prefer to cut it 2 halfs or inject it with NBF 10% or whole processing is the best ?? Thanks in advance Mohamed From Fawn.Bomar at HalifaxRegional.com Thu Feb 25 12:39:37 2016 From: Fawn.Bomar at HalifaxRegional.com (Fawn Bomar) Date: Thu, 25 Feb 2016 18:39:37 +0000 Subject: [Histonet] Professiional competency Message-ID: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA1A500D@EXCH-2K10.hrhs.com> Does anyone have a policy for assessing professional competency and a log for recording the assessment for pathologists who provide interpretive services to the lab that they would be willing to share? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From making at ufl.edu Thu Feb 25 12:41:37 2016 From: making at ufl.edu (King,Michael A) Date: Thu, 25 Feb 2016 18:41:37 +0000 Subject: [Histonet] metal enhanced DAB Message-ID: Tyrone, I've had better luck using acetate buffer with metal-enhanced DAB. There's less of a precipitation problem. You can also dilute the reaction solution and the reaction product will develop more slowly, or just decrease the peroxide concentration. You also have some control over the color by adjusting the pH; Co can range from blue to black and Ni from brown to black. Good luck, Mike King ----------------- Wed, 24 Feb 2016 16:08:00 -0600 Tyron Genade wrote: Subject: [Histonet] metal enhanced DAB Message-ID: Hello, I'm using the Co/Ni enhanced DAB from Histological & Histochemical Methods (2nd Ed). Does the solution normally turn a blue-gray after adding the H2O2? I am getting a lot of back ground and precipitate on the sections. The pH is 7.3. I had used a similar method for Western Blotting that took 5 minutes for the solution to change color. This is a near instantaneous color change. Thanks Tyrone From Megan.Dishop at childrensmn.org Thu Feb 25 13:12:25 2016 From: Megan.Dishop at childrensmn.org (Megan Dishop) Date: Thu, 25 Feb 2016 13:12:25 -0600 Subject: [Histonet] Professiional competency In-Reply-To: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA1A500D@EXCH-2K10.hrhs.com> References: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA1A500D@EXCH-2K10.hrhs.com> Message-ID: <56CEFDB9.D202.00E3.1@childrensmn.org> Fawn, At Children's Minnesota, this is managed and tracked at the hospital level via ongoing and focused professional practice evaluation (OPPE, FPPE). We have chosen specific elements of our anatomic pathology quality program to forward to the hospital and then these are incorporated into a semi-annual assessment form, and reviewed by a hospital committee. Our internal laboratory policy reflects this practice and lists some of the specific quality metrics we have chosen, but your lab leadership and pathology group should select criteria that seem most appropriate for your practice setting. See our attached policy as an example. Megan K. Dishop MD Medical Director, Pediatric Anatomic Pathology Children's Hospitals and Clinics of Minnesota Laboratories 2525 Chicago Ave S. MS32-B600, Minneapolis, MN 55404 USA Phone: 612-813-6521 Fax: 612-813-7721 Email: megan.dishop at childrensMN.org Adjoint Professor of Pediatrics, University of Colorado School of Medicine >>> Fawn Bomar via Histonet 2/25/2016 12:39 PM >>> Does anyone have a policy for assessing professional competency and a log for recording the assessment for pathologists who provide interpretive services to the lab that they would be willing to share? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. From jford at cytomx.com Thu Feb 25 18:36:09 2016 From: jford at cytomx.com (Judi Ford) Date: Fri, 26 Feb 2016 00:36:09 +0000 Subject: [Histonet] Image analysis software Message-ID: Hi everyone, We are in the middle of looking at image analysis software and I was wondering what software programs people are using. Why did you pick the one you are using? Are you using it for brightfield whole slide analysis or for fluorescent slide analysis? What file types can be used with your software? I would love to hear your experiences. Thanks, Judi From hhellinger at gmail.com Thu Feb 25 19:04:51 2016 From: hhellinger at gmail.com (Hilllel Hellinger) Date: Thu, 25 Feb 2016 20:04:51 -0500 Subject: [Histonet] Reagent bottles VIP 5 Message-ID: I need used reagent bottles for VIP 5 Does anyone have? -- Hillel Hellinger North Miami Beach, Fl 33162 Tel# 305-992-1348 From hhellinger at gmail.com Thu Feb 25 19:06:53 2016 From: hhellinger at gmail.com (Hilllel Hellinger) Date: Thu, 25 Feb 2016 20:06:53 -0500 Subject: [Histonet] VIP reagent bottles Message-ID: I need used reagent bottles for VIP 5 Does anyone have? Hillel Hellinger 305-992-1348 hhellinger at gmail.com From cforster at umn.edu Thu Feb 25 19:12:44 2016 From: cforster at umn.edu (Colleen Forster) Date: Thu, 25 Feb 2016 19:12:44 -0600 Subject: [Histonet] VIP reagent bottles In-Reply-To: References: Message-ID: I need these too...one for sure....am following your replies... C On Thu, Feb 25, 2016 at 7:06 PM, Hilllel Hellinger via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I need used reagent bottles for VIP 5 > Does anyone have? > > Hillel Hellinger > 305-992-1348 > hhellinger at gmail.com > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From carl.hobbs at kcl.ac.uk Fri Feb 26 01:43:53 2016 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 26 Feb 2016 07:43:53 +0000 Subject: [Histonet] Human nuclear antibody MAB1281 Message-ID: I had/have the same problem with that ab on FFPWS +/- AR Also negative, under same conditions: Serotec's 6970-1257, Abcam's ab5675 I use 2 abs on hu teratomas in mouse host: Takara clontech STEM121 antibody Abcam anti MTCO2 ab110258 Images to be seen on Histonet Images site Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From j.benavides at eae.csic.es Fri Feb 26 03:29:28 2016 From: j.benavides at eae.csic.es (Julio Benavides) Date: Fri, 26 Feb 2016 10:29:28 +0100 Subject: [Histonet] Image analysis software In-Reply-To: References: Message-ID: <20160226102928.Horde.kZWVOn5sH6rUHwjzEp8Egw1@webmail.csic.es> Hi Jodi, I was using imageAnalysis from Olympus some years ago, bus since I discovered ImageJ there is no way I will go back to commercial stuff. Pros of ImageJ, apart from the obvious one that it is free, is that a lot of people is using it, so plenty of tutorials, tricks, how-to in internet. Also, there are plenty of add-on to this software so there will be always one application for your needs. You don't need a big computer to work with it. Only downside, if you can consider it, is that the looks of the software are not as fancy as the commercial ones. I use it for image analysis in IHC and IHF. Hope this helps. i also would be happy to know what other people thinks regarding this issue. Cheers Julio Judi Ford via Histonet escribi?: > Hi everyone, > We are in the middle of looking at image analysis software and I was > wondering what software programs people are using. Why did you pick > the one you are using? Are you using it for brightfield whole slide > analysis or for fluorescent slide analysis? What file types can be > used with your software? > > I would love to hear your experiences. > > Thanks, > Judi > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joost.bruijntjes at tno.triskelion.nl Fri Feb 26 03:59:38 2016 From: joost.bruijntjes at tno.triskelion.nl (Bruijntjes, J.P. (Joost)) Date: Fri, 26 Feb 2016 09:59:38 +0000 Subject: [Histonet] auto-fluorescence Message-ID: Hi histonetters I hope one of you can help me. I don't have any experience with fluorescence. We are searching for some components and we will use an alexa488 conjugated secondary. Will there be an auto-fluorescence on different organs (brain/spleen/liver)? Greetings Joost From melissa at alliedsearchpartners.com Fri Feb 26 05:53:11 2016 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Fri, 26 Feb 2016 11:53:11 +0000 Subject: [Histonet] Direct Hire for Histotech Job in Houston, TX Message-ID: Happy Friday Everyone! I have a position in Houston, TX for Full Time/Permanent. The shift is Evening Shift. Please contact me for job description. Have a great day! Melissa Owens President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 F: 888.388.7572 From annamhuntley at gmail.com Fri Feb 26 07:50:39 2016 From: annamhuntley at gmail.com (Anna Huntley Coffey) Date: Fri, 26 Feb 2016 08:50:39 -0500 Subject: [Histonet] Suggestions for staining bacteria in FFPE decaled growth plate Message-ID: Happy Friday, Histonetters! I have a puzzle that I could really use your expert knowledge on this morning. One of our pathologists has a decalcified FFPE mouse bone embedded longitudinally and has found some bacteria located in the growth plate. The bacteria has been confirmed with fluorescence, but she really wants to be able to show the morphology of the tissue with the bacteria. So far, H&E, gram, Brown & Hopps stain the growth plate so dark that you can't make out the bacteria. My suggestions were to try a thinner section and adjust the timing of the H&E (haven't had a chance to do that yet), but I wanted to throw the question out to you all to see if anyone has tried other stains or techniques for this type of issue. I'd really appreciate the help! Thanks, Anna From b-frederick at northwestern.edu Fri Feb 26 07:57:17 2016 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Fri, 26 Feb 2016 13:57:17 +0000 Subject: [Histonet] CAP Message-ID: <51f5f4e767224e6889dfd4ba7b2a777e@evcspmbx03.ads.northwestern.edu> ANP21382. What kind of policy or SOP do you all have for this question? T asks how reagents are given an expiration. This includes but is not restricted to reagents where manufacturer does not specify a date. We date made up reagents as a 6 month expiration unless we know it doesn't last that long. Came up during interim self inspection. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu From blayjorge at gmail.com Fri Feb 26 08:15:57 2016 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Fri, 26 Feb 2016 09:15:57 -0500 Subject: [Histonet] Suggestions for making glutaraldehyde penetrate in between little setae of insects for microscopy Message-ID: Hello Histonetetrs: I am trying to fix insect wings for TEM. Less than ideal fixation appears to be caused by abundant setae on the surface of the wings. Those setae, trap air, thus making penetration of the fixative more difficult difficult. Interestingly, also the wings tend to curl upon themselves. Options re. fixation: 1. Just submerging the insects mechanically does not seem to solve the problem. 2. We are contemplating submerging and evacuating (intermittently) a few times for 2 minutes (vacuuming) 3. Are there chemicals that can break the bubbles formed in between the setae? 4. Any other comments or suggestions? Options to minimize (ideally eliminate) curling of the wings? If you have any constructive suggestions, please kindly email it to me directly. blayjorge at gmail.com Of course, we intend to begin with new freshly collected insects. Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From gayle.callis at bresnan.net Fri Feb 26 10:09:14 2016 From: gayle.callis at bresnan.net (Gayle Callis) Date: Fri, 26 Feb 2016 09:09:14 -0700 Subject: [Histonet] auto-fluorescence In-Reply-To: References: Message-ID: <000601d170b0$09e32090$1da961b0$@bresnan.net> Dear Joost, Yes and to read up on this, go to this website for an excellent, well referenced free pdf i.e. Autofluorescence, causes and cures. https://www.google.com/?gws_rd=ssl#q=autofluorescence+causes+and+cures+wrigh t+cell+imaging+facility They also posted a pdf on mounting medias for fluorescence microscopy work. The pdf will tell you what tissue and cellular components autofluoresce. The authors also provided various methods to reduce or remove autofluorescence of these components but also how to remove aldehyde induced autofluorescence if you are fixing tissues with NBF or PFA. Remember there is no autofluroescence in the Near Infra Red region so one can use an NIR fluorophore, i.e. Alexa 750 (red) or another fluorophore for the NIR region, and even use tissue autofluorescence as a "counterstain fluorescence" However, you cannot see these with the human eye but these photograph beautifully. OR if you have a spectral imaging or confocal capabilities, you can rule out autofluorescence without having to treat the tissue section. If you have difficulty access this pdf, I will send via private email. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT USA -----Original Message----- From: Bruijntjes, J.P. (Joost) via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, February 26, 2016 3:00 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] auto-fluorescence Hi histonetters I hope one of you can help me. I don't have any experience with fluorescence. We are searching for some components and we will use an alexa488 conjugated secondary. Will there be an auto-fluorescence on different organs (brain/spleen/liver)? Greetings Joost _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbruno3 at buffalo.edu Fri Feb 26 10:11:11 2016 From: mbruno3 at buffalo.edu (Michael Bruno) Date: Fri, 26 Feb 2016 11:11:11 -0500 Subject: [Histonet] Suggestions for alternative fluorescent nuclei stains (that are not DAPI) ? Message-ID: <56D0791F.1040206@buffalo.edu> Hello all, I perform immunohistochemistry (IHC) and immunofluorescence (IF) on fixed free-floating rat brain sections (~50um thickness). Currently I use Prolong Gold with DAPI as a mounting media as well as a stain for the nuclei. I am seeking an alternative (fluorescent) nuclei stain for free floating rat brain sections. The issue I am having is that one of my fluorescent markers (FluoroGold) has native fluorescence in the UV channel. Since it is the same channel that DAPI is in, this will not do. I do not have an antibody or fluorescent marker in the far-red channel so I would really like to find one in that area. I have looked at To-Pro-3 and NucRed Dead 647 as alternatives because their fluorescence is in the far-red channel. However I am unclear if these stains can be used on brain sections and not just in vitro cells. To summarize, I am looking to find an alternative nuclei stain to DAPI, and one that is preferably in the far-red channel. If anyone here has a protocol for a nuclei stain that they would not mind sharing, I would be very appreciative as well. Thank you so much for your help, Mike Bruno State University of New York at Buffalo From BZIMMERM at gru.edu Fri Feb 26 10:17:31 2016 From: BZIMMERM at gru.edu (Zimmerman, Billie) Date: Fri, 26 Feb 2016 16:17:31 +0000 Subject: [Histonet] HISTOPALOOZA III Georgia Society for Histology Message-ID: Histopalooza! III welcomes Ely Klar, MS,HTL(ASCP) back as a presenter. Ely is an instructor at Columbus State University in Columbus, Georgia, home of the Cougars. (Cougars being the school mascot, not where all single women over 40 live.) Her workshop on microscopic anatomy is always well attended. It's a great workshop for someone sitting for the HT/HTL exams or for anyone that needs a refresher. Ely has a warm personality and people truly enjoy her presentation. Warm regards, Billie Zimmerman (too old to be a cougar) GSH secretary From gayle.callis at bresnan.net Fri Feb 26 10:52:38 2016 From: gayle.callis at bresnan.net (Gayle Callis) Date: Fri, 26 Feb 2016 09:52:38 -0700 Subject: [Histonet] glycine method to remove aldehyde induced autofluorescence Message-ID: <000001d170b6$19c95a00$4d5c0e00$@bresnan.net> Dear All, Some person kindly mentioned my name as a source for the glycine method to remove aldehyde induced autofluorescence. We liked the simplicity of this method, plus gentle to tissue sections. This was the original information but we modified it. I have seen concentrations of glycine range from 100 mM to 700 mM . Original method: 1. Rehydrated tissue sections: A Tris-glycine mixture (adjust 0.1M glycine to pH 7.2-7.4 with 1M Tris base will saturate free aldehyde groups. (15-30 minutes at room temp in Tris-glycine for FFPE sections. Wash well in PBS. If the tissue is fragile though, only use the Tris-glycine method. 2.. For tissues coming out of formalin, soak the tissues for 30 min to 1 hour and rinse well. Callis Modified Method: We did not use TRIS-glycine, preferring the same buffer used in IF staining. Make fresh for a day's use. 500 mM glycine in pH 7.2 - 7.4 Dulbeccos PBS (Sigma). We increased concentration to 500 mM glycine for 15 - 30 minutes at RT after we found 100 mM reduced autofluorescence while the higher concentration did a better more complete removal. I don't think it makes much difference if you use TBS or DPBS so you can use whatever your lab prefers for IHC/IF staining should work equally well. We found two changes of glycine solution worked well since you are refreshing the solution to sop up those free aldhydes. Do 15 minutes incubation for each change, don't rinse the sections between changes, just tip, drain slides, blotted edges of sections, add solution on sections with slides laying flat on a manual stainer. Some people might prefer glycine solution in a coplin jar if they are going to an automated staining system. If you fear drying, one method was 700mM glycine, 0.15% BSA (use pure IgG and protease free), and 0.1% sodium azide in PBS with 15 to 30 min RT incubation. Sodium azide can be left out since it is there to prevent bacterial growth, and deemed unnecessary since our glycine solution was made fresh before a one day/one time use. Glycine is cheap and goes into solution readily. FYI, lysine has also been used to get rid of free aldehydes (Elias J. Immunohistopathology book) Good luck Gayle Callis HTL/HT/MT(ASCP) Bozeman MT From liz at premierlab.com Fri Feb 26 11:00:38 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Fri, 26 Feb 2016 10:00:38 -0700 Subject: [Histonet] CAP In-Reply-To: <51f5f4e767224e6889dfd4ba7b2a777e@evcspmbx03.ads.northwestern.edu> References: <51f5f4e767224e6889dfd4ba7b2a777e@evcspmbx03.ads.northwestern.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE02854EF5433F@SBS2K8.premierlab.local> Bernice We are not a clinical lab, we are a GLP compliant lab and we have a procedure that addresses this and everything else about reagent preparation. I will put the procedure and forms that we use on the NSH BLOCK for anyone who is interested. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Bernice Frederick via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, February 26, 2016 6:57 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CAP ANP21382. What kind of policy or SOP do you all have for this question? T asks how reagents are given an expiration. This includes but is not restricted to reagents where manufacturer does not specify a date. We date made up reagents as a 6 month expiration unless we know it doesn't last that long. Came up during interim self inspection. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff at uropartners.com Fri Feb 26 11:40:41 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 26 Feb 2016 17:40:41 +0000 Subject: [Histonet] Friday Fun (non-work related blog post, easy to ignore) Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B6F75E3@COLOEXCH01.uropartners.local> Anyone moving to Chicago area? http://www.chicagonow.com/downsize-maybe/2016/02/to-the-family-who-will-buy-our-house-whoever-you-are/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From jclark at pcnm.com Fri Feb 26 11:44:37 2016 From: jclark at pcnm.com (Joanne Clark) Date: Fri, 26 Feb 2016 17:44:37 +0000 Subject: [Histonet] H. pylori by IHC Message-ID: <7A7BDD92B984E847A7E71BC9C00A66D3126F08C3@S11MAILD034N2.sh11.lan> Recently someone placed a query regarding the precipitate with Cell Marques H. pylori marker. The response was to filter it before use to clean it up. May I ask how it is being filtered? We have the same problem and it makes the slide difficult to interpret. Joanne Clark, HT BAAS Director of Histology Pathology Consultants of New Mexico Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From liz at premierlab.com Fri Feb 26 13:03:38 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Fri, 26 Feb 2016 12:03:38 -0700 Subject: [Histonet] Image analysis software In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02854EF54345@SBS2K8.premierlab.local> Judi Through the years we have used a wide variety of software applications, we have been performing image analysis since 2006, we started whole slide scanning in 2007. We have used various modules from Zeiss, Image Pro, Aperio's Toolbox, Definiens and free shareware such at Image J. There are also new vendors on the market such as Indica Labs and Visopharm. I think you need to make an educated decision based upon what your needs are and how complex your analysis is and the price of the product, some of the commercially available software packages can be expensive. I saw the earlier post that discussed Image J and that might be a good option to start with if you have the time and ability to learn how to work with the software, you would be surprised to learn that Image J can do quite a bit. The only thing that it may not be capable of is pattern recognition, others that use the software can comment on that aspect. I do know that it is easier to trace in Image J than it is in some of the viewer software that are linked to a particular scanner. The other software solutions may be a bit easier to understand and work with initially but there will always be a learning curve associated with any software package that you choose to use. Most software packages will support different file types, such as tiff or modified tiff, you will need to make sure the image format your scanner generates is compatible with the software you choose. My suggestion would be to look at all of them and determine what is best for your use cases. I would also be a bit forward thinking, you might think that you only need the software to do certain things but as you use the technology more you will see that you will want to use if for other use cases. The other thing I want to comment on is that the success of your image analysis is not just related to the software that you choose to use but also the quality, consistency and reproducibility of your histology preparations. If you want more information on the topic I would suggest going to the DPA - Digital Pathology Association website, it has a list of most of the vendors in the space and if you become of member, its $50.00 for technologists, you will have access to all of the past presentations from the Pathology Visions conferences and webinars. Here is the link to the website. The other option is to attend the Pathology Visions conference, it a conference geared towards digital pathology. If you are a NSH member there is a scholarship available to attend the conference, you could apply for that. The links to the NSH scholarship and the Pathology Visions conference are below. The list of vendors is under the resources tab. https://digitalpathologyassociation.org/ https://digitalpathologyassociation.org/pathology-visions-2016 http://nsh.org/content/digital-pathology-association-scholarship-pathology-vision Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Judi Ford via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 25, 2016 5:36 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Image analysis software Hi everyone, We are in the middle of looking at image analysis software and I was wondering what software programs people are using. Why did you pick the one you are using? Are you using it for brightfield whole slide analysis or for fluorescent slide analysis? What file types can be used with your software? I would love to hear your experiences. Thanks, Judi _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doolee at shands.ufl.edu Fri Feb 26 13:10:38 2016 From: doolee at shands.ufl.edu (Dooley, Elaine) Date: Fri, 26 Feb 2016 19:10:38 +0000 Subject: [Histonet] h. pylori Message-ID: <6193C53146742E4586DD4838F47F79AE0EA2C2BA@MSXMB02.Shands.local> Hi histonetters, We filter the H.pylori antibody with the filters that come with the Ventana Prep kits. Elaine Dooley Shands Teaching Hospital 352-265-0111 ext 72117 From jaylundgren at gmail.com Fri Feb 26 13:40:25 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 26 Feb 2016 13:40:25 -0600 Subject: [Histonet] Suggestions for making glutaraldehyde penetrate in between little setae of insects for microscopy In-Reply-To: References: Message-ID: A little alcohol will lower the surface tension of aqueous solutions. Then there's always DMSO. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Feb 26, 2016 at 8:15 AM, Jorge A. Santiago-Blay via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello Histonetetrs: > > I am trying to fix insect wings for TEM. Less than ideal fixation appears > to be caused by abundant setae on the surface of the wings. Those setae, > trap air, thus making penetration of the fixative more difficult difficult. > Interestingly, also the wings tend to curl upon themselves. > > Options re. fixation: > 1. Just submerging the insects mechanically does not seem to solve the > problem. > > 2. We are contemplating submerging and evacuating (intermittently) a few > times for 2 minutes (vacuuming) > > 3. Are there chemicals that can break the bubbles formed in between the > setae? > > 4. Any other comments or suggestions? > > Options to minimize (ideally eliminate) curling of the wings? > > If you have any constructive suggestions, please kindly email it to me > directly. > > blayjorge at gmail.com > > Of course, we intend to begin with new freshly collected insects. > > Gratefully, > > Jorge > > Jorge A. Santiago-Blay, PhD > blaypublishers.com > > 1. Positive experiences for authors of papers published in *LEB* > http://blaypublishers.com/testimonials/ > > 2. Free examples of papers published in *LEB*: > http://blaypublishers.com/category/previous-issues/. > > 3. *Guidelines for Authors* and page charges of *LEB*: > http://blaypublishers.com/archives/ *.* > > 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ > > > http://blayjorge.wordpress.com/ > http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From simmca at UPMC.EDU Fri Feb 26 13:42:41 2016 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Fri, 26 Feb 2016 19:42:41 +0000 Subject: [Histonet] Suggestions for making glutaraldehyde penetrate in between little setae of insects for microscopy In-Reply-To: References: , Message-ID: <8C8949D6-0BA3-4A65-9331-F771ADF64127@UPMC.EDU> Double PPE if you use DMSO Sent from my iPhone > On Feb 26, 2016, at 2:41 PM, Jay Lundgren via Histonet wrote: > > A little alcohol will lower the surface tension of aqueous solutions. Then > there's always DMSO. > > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > > On Fri, Feb 26, 2016 at 8:15 AM, Jorge A. Santiago-Blay via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Hello Histonetetrs: >> >> I am trying to fix insect wings for TEM. Less than ideal fixation appears >> to be caused by abundant setae on the surface of the wings. Those setae, >> trap air, thus making penetration of the fixative more difficult difficult. >> Interestingly, also the wings tend to curl upon themselves. >> >> Options re. fixation: >> 1. Just submerging the insects mechanically does not seem to solve the >> problem. >> >> 2. We are contemplating submerging and evacuating (intermittently) a few >> times for 2 minutes (vacuuming) >> >> 3. Are there chemicals that can break the bubbles formed in between the >> setae? >> >> 4. Any other comments or suggestions? >> >> Options to minimize (ideally eliminate) curling of the wings? >> >> If you have any constructive suggestions, please kindly email it to me >> directly. >> >> blayjorge at gmail.com >> >> Of course, we intend to begin with new freshly collected insects. >> >> Gratefully, >> >> Jorge >> >> Jorge A. Santiago-Blay, PhD >> blaypublishers.com >> >> 1. Positive experiences for authors of papers published in *LEB* >> http://blaypublishers.com/testimonials/ >> >> 2. Free examples of papers published in *LEB*: >> http://blaypublishers.com/category/previous-issues/. >> >> 3. *Guidelines for Authors* and page charges of *LEB*: >> http://blaypublishers.com/archives/ *.* >> >> 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ >> >> >> http://blayjorge.wordpress.com/ >> http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From probbins at pekinhospital.com Fri Feb 26 14:19:59 2016 From: probbins at pekinhospital.com (Pauletta A. Robbins) Date: Fri, 26 Feb 2016 20:19:59 +0000 Subject: [Histonet] unsubscribe Message-ID: Please unsubscribe my email address Thank you! Pauletta A. Robbins From smallwood.john at gmail.com Sat Feb 27 14:23:22 2016 From: smallwood.john at gmail.com (John Smallwood) Date: Sat, 27 Feb 2016 15:23:22 -0500 Subject: [Histonet] Routine lab Formalin Fixation Message-ID: What are people using for minimum fixation times in NBF 10% for tissues in a routine mixed sample load Histology Lab, on a routine VIP 5000 processor I have concerns1.) for Bio-safety,2.) Immunohistochemistry, 3.) Routine samples nuclear staining (no alcohol fixation ),4.) Sample firmness prior to grossing. We handle approximately 6000mixed samples a year. The gamut ranges from Breast,Bowel to ECC's. The technologists have been given the responsibility within the last year for grossing or sampling smaller samples. - Bx's, skins and Hernia sacs. The Management has been more concerned about turn around times for Surgical samples, than any of the other Scientific concerns noted above. This journal article is purported to recommend minimum 24 hours fixation; Goldstein NS, Hewitt SM, Taylor CR, Yaziji H, Hicks DG. Recom- mendation for improved standardization of immunohistochemist- ry. Appl Immunohistochemistry Mol Morphol 2007; 15:124-33. From smallwood.john at gmail.com Sun Feb 28 07:54:20 2016 From: smallwood.john at gmail.com (John Smallwood) Date: Sun, 28 Feb 2016 08:54:20 -0500 Subject: [Histonet] Politics in the Lab ; Turn around times vs Scientific sanity Message-ID: What are people using for minimum fixation times in NBF 10% for tissues in a routine mixed sample load Histology Lab, on a routine VIP 5000 processor I have concerns1.) for Bio-safety,2.) Immunohistochemistry, 3.) Routine samples nuclear staining (no alcohol fixation ),4.) Sample firmness prior to grossing. We handle approximately 6000mixed samples a year. The gamut ranges from Breast,Bowel to ECC's. The technologists have been given the responsibility within the last year for grossing or sampling smaller samples. - Bx's, skins and Hernia sacs. The Management has been more concerned about turn around times for Surgical samples, than any of the other Scientific concerns noted above. This journal article is purported to recommend a minimum of 24 hours fixation; Goldstein NS, Hewitt SM, Taylor CR, Yaziji H, Hicks DG. Recom- mendation for improved standardization of immunohistochemist- ry. Appl Immunohistochemistry Mol Morphol 2007; 15:124-33. John Smallwood, MLT. STEGH Gen. Hosp. St.Thomas, On. Canada From criley at dpspa.com Mon Feb 29 05:15:54 2016 From: criley at dpspa.com (Charles Riley) Date: Mon, 29 Feb 2016 06:15:54 -0500 Subject: [Histonet] H&E staining Message-ID: Hello all, We've been running into an issue lately with our H&E staining. The hematoxylin and eosin are light on and off but the chromatin is consistently staining light. I have changed the time in hematoxylin to longer, used a fresh lot of both hematoxylin and our clarifier solution and the stain is not improving. Does anyone have any suggestions? Here is our routine stain line procedure. 65 degree Celsius oven for 20 minutes 2x xylene for 5 mins 2x 100% alcohol for 1 minute 95% alcohol for 1 minute DI water rinse for 1 minute Richard Allan scientific hematoxylin for 4 minutes DI water rinse for 2 minutes Richard Allan Clarifier 1 for 2.5 minutes DI water for 2 minutes Bluing Reagent for 1 minute DI water rinse for 2 minutes 95% alcohol for 1 minute Eosin Y for 2 minutes 95% alcohol for 30 seconds 2x 100% alcohol for 1 minute each 2x xylene for 1 minute each Mount and coverslip. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE From LRaff at uropartners.com Mon Feb 29 07:12:19 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 29 Feb 2016 13:12:19 +0000 Subject: [Histonet] Job openning in Chicago Suburbs Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B705FC0@COLOEXCH01.uropartners.local> Our lab, a large private uropathology lab in the west Chicago suburbs, is seeking a full time day shift histologist. Please contact me for details. Please spread the word! Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From latecor at adinet.com.uy Mon Feb 29 07:31:35 2016 From: latecor at adinet.com.uy (Carlos Defeo) Date: Mon, 29 Feb 2016 13:31:35 +0000 Subject: [Histonet] H&E staining Message-ID: Charles,extend time on the deparaffinization station,and even better,renew solvents at this point also. My kind regards, Carlos Defeo Histotechnologist From rjbuesa at yahoo.com Mon Feb 29 08:27:25 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 29 Feb 2016 14:27:25 +0000 (UTC) Subject: [Histonet] H&E staining In-Reply-To: References: Message-ID: <1567788252.777382.1456756045073.JavaMail.yahoo@mail.yahoo.com> Usually inconsistency in H&E staining has two different sources: either the staining process itself, or the previous tissue processing sequence.Your staining protocol has a "standard" sequence but I would add an additional xylene and would increase the time in hemtoxylin to 7 minutes for you will differentiate ant way with the clarifier. Are you staining manually? If so the time in the clarifier should not be standard, the stained sections should remain in it until no more color leaches from the sections and should be as short as possible. Most likely your staining problems resides in the "clarifier" step and when the sections are of the "right" tissue type and the "right" thickness the staining is OK, but when they are either too thin or of tissues with less amounts of nuclei, the staining is faint. In any even, I strongly suggest you to change your whole protocol and eliminate xylene and alcohols in the dewaxing step, and switch to a 2% aq. sol. of dishwashing soap at 90?C (2 stations x 2 min. each) ? hot distilled water (90?C x 1 ? 45?C x 1 min) ? dist. water at room temp. which will allow you not only eliminating xylene but also ?finish the whole procedure sooner and much cheaper.Try it! Ren? On Monday, February 29, 2016 6:33 AM, Charles Riley via Histonet wrote: Hello all, We've been running into an issue lately with our H&E staining. The hematoxylin and eosin are light on and off but the chromatin is consistently staining light. I have changed the time in hematoxylin to longer, used a fresh lot of both hematoxylin and our clarifier solution and the stain is not improving.? Does anyone have any suggestions? Here is our routine stain line procedure. 65 degree Celsius oven for 20 minutes 2x xylene for 5 mins 2x 100% alcohol for? 1 minute 95% alcohol for 1 minute DI water rinse for 1 minute Richard Allan scientific hematoxylin for 4 minutes DI water rinse for 2 minutes Richard Allan Clarifier 1 for 2.5 minutes DI water for 2 minutes Bluing Reagent for 1 minute DI water rinse for 2 minutes 95% alcohol for 1 minute Eosin Y for 2 minutes 95% alcohol for 30 seconds 2x 100% alcohol for 1 minute each 2x xylene for 1 minute each Mount and coverslip. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MICHELE.FRENCH at bms.com Mon Feb 29 11:12:37 2016 From: MICHELE.FRENCH at bms.com (FRENCH, MICHELE) Date: Mon, 29 Feb 2016 17:12:37 +0000 Subject: [Histonet] NJ Histology Meeting in June Message-ID: <0cf99f07da5f48cfa117b4dfe4ab8b85@DM2PR26MB0030.067d.mgd.msft.net> Dear Histonet Members, I wanted to let you know that The New Jersey Society for Histotechnology is planning to host a two-day Summer Meeting June 10-11 at the Wyndham Hotel in Mount Laurel, NJ. More information will be available at the end of March, and meeting registration will start on April 1st. SPEAKERS NEEDED ASAP: We need to fill out last three time slots: (one 1.5hr session, one 3hr session and one 45minute session). VENDORS NEEDED: We only have 14 tables left in the exhibit area. Reservations are on a first-come, first-served basis. JOB LISTINGS WANTED: If you have a job opening that you would like posted on our Employment Opportunities board during the event, just let us know! Please contact Michele French by Email Michele.French at bms.com or Phone 609-818-3873. ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From histo at pathlab.us Mon Feb 29 11:29:34 2016 From: histo at pathlab.us (Histology) Date: Mon, 29 Feb 2016 17:29:34 +0000 Subject: [Histonet] Freeze Spray Ban Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD823433DD@vdc01.domain.local> Hi All, Just got an alert stating that the EPA is banning HFCs found in Freeze Spray effective July 20th. Does anybody use a spray that does not contain this banned chemical? If so, please let me know where you get it. Thanks, Mehndi Helgren Dominion Pathology Labs "DPL" From criley at dpspa.com Mon Feb 29 13:00:14 2016 From: criley at dpspa.com (Charles Riley) Date: Mon, 29 Feb 2016 14:00:14 -0500 Subject: [Histonet] Freeze Spray Ban In-Reply-To: <09CFA3F99D5B2B42B88CDFB2FC4CFD823433DD@vdc01.domain.local> References: <09CFA3F99D5B2B42B88CDFB2FC4CFD823433DD@vdc01.domain.local> Message-ID: We use Stoner Multipurpose freeze spray. 100% pure virgin tetrafluoroethane On Mon, Feb 29, 2016 at 12:29 PM, Histology via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi All, > > Just got an alert stating that the EPA is banning HFCs found in Freeze > Spray effective July 20th. Does anybody use a spray that does not contain > this banned chemical? If so, please let me know where you get it. > > Thanks, > > Mehndi Helgren > Dominion Pathology Labs "DPL" > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE From brett_connolly at merck.com Mon Feb 29 13:21:20 2016 From: brett_connolly at merck.com (Connolly, Brett M) Date: Mon, 29 Feb 2016 14:21:20 -0500 Subject: [Histonet] Masson's Trichrome or Sirius Red on frozen tissue Message-ID: Has anyone preformed these stains of frozen sections??? Looking for any tips. I'll have fresh frozen tissue that can be fixed after sectioning and mounting onto the slide. Thanks, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From sbonner at pathregional.com Mon Feb 29 14:09:32 2016 From: sbonner at pathregional.com (Silvia Bonner) Date: Mon, 29 Feb 2016 20:09:32 +0000 Subject: [Histonet] automatic stainers and tissue processors Message-ID: Hello, We are looking into purchasing new automatic H&E strainers and new tissue processors. Any helpful advice? I know this may have been discussed before but I an new to histonet. Thanks for your help! Silvia Bonner, HT(ASCP) CM Histology Supervisor sbonner at pathregional.com Pathologists' Regional Laboratory 1225 Highland Ave Clarkston, WA 99403 This email may contain physician, patient and/or attorney material that is confidential or privileged for the sole use of the intended recipient. YOU ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED. If you are not the intended recipient, please notify us immediately by telephone at (208) 746-0516 or (509) 758-5576 and delete all copies. From j.rowaihi at alborglaboratories.com Mon Feb 29 15:24:08 2016 From: j.rowaihi at alborglaboratories.com (Jamal Rowaihi) Date: Tue, 01 Mar 2016 00:24:08 +0300 Subject: [Histonet] automatic stainers and tissue processors Message-ID: Go for Sakura products with no hesitate.? Regards Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories?Sent from my cell phone-------- Original message --------From: Silvia Bonner via Histonet Date: 2/29/2016 11:09 PM (GMT+03:00) To: histonet at lists.utsouthwestern.edu Subject: [Histonet] automatic stainers and tissue processors Hello, We are looking into purchasing new automatic H&E strainers and new tissue processors.? Any helpful advice?? I know this may have been discussed before but I an new to histonet. Thanks for your help! Silvia Bonner, HT(ASCP) CM Histology Supervisor sbonner at pathregional.com Pathologists' Regional Laboratory 1225 Highland Ave Clarkston, WA 99403 This email may contain physician, patient and/or attorney material that is confidential or privileged for the sole use of the intended recipient. YOU ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED.? If you are not the intended recipient, please notify us immediately by telephone at (208) 746-0516 or (509) 758-5576 and delete all copies. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tejohnson at genoptix.com Mon Feb 29 15:34:31 2016 From: tejohnson at genoptix.com (Teri Johnson) Date: Mon, 29 Feb 2016 21:34:31 +0000 Subject: [Histonet] H&E staining Message-ID: Hi Charles, If my memory serves, Clarifier 1 and Clarifier 2 are different mostly in that one is an alcoholic solution and the other an aqueous one. So they both behave differently in their mechanism of action. The alcohol based reagent should remove more hematoxylin from the cells than the aqueous based reagent. Also if you do a google search for Richard Allan Clarifier 1, you should find a pdf document that outlines what their stain series are, and how to use (time) them. It's a great document that has a couple charts on how to increase or decrease stain contrast. It appears that they recommend 30 seconds-1 minute for either solution for the Hematoxylin 7211 and 7212 stain, and 20 seconds-1 minute if using the Hematoxylin 1 and 2. At 2.5 minutes, you might be overdoing your "clarifying"! Best wishes and good luck! Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From k.leigh.adams.865 at gmail.com Mon Feb 29 16:14:01 2016 From: k.leigh.adams.865 at gmail.com (k.leigh.adams.865) Date: Mon, 29 Feb 2016 17:14:01 -0500 Subject: [Histonet] Histonet Digest, Vol 147, Issue 31 Message-ID: <2qaug89bsn3ur5vd65k4vsiv.1456784041523@email.android.com> Ted Pella, pelco freeze spray. Sent via the Samsung Galaxy Tab? 4, an AT&T 4G LTE tablet -------- Original message -------- From: histonet-request at lists.utsouthwestern.edu Date: 2/29/2016 1:00 PM (GMT-05:00) To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 147, Issue 31 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ?? 1. H&E staining (Charles Riley) ?? 2. Job openning in Chicago Suburbs (Lester Raff MD) ?? 3. H&E staining (Carlos Defeo) ?? 4. Re: H&E staining (Rene J Buesa) ?? 5. NJ Histology Meeting in June (FRENCH, MICHELE) ?? 6. Freeze Spray Ban (Histology) ---------------------------------------------------------------------- Message: 1 Date: Mon, 29 Feb 2016 06:15:54 -0500 From: Charles Riley To: histonet at lists.utsouthwestern.edu Subject: [Histonet] H&E staining Message-ID: Content-Type: text/plain; charset=UTF-8 Hello all, We've been running into an issue lately with our H&E staining. The hematoxylin and eosin are light on and off but the chromatin is consistently staining light. I have changed the time in hematoxylin to longer, used a fresh lot of both hematoxylin and our clarifier solution and the stain is not improving.? Does anyone have any suggestions? Here is our routine stain line procedure. 65 degree Celsius oven for 20 minutes 2x xylene for 5 mins 2x 100% alcohol for? 1 minute 95% alcohol for 1 minute DI water rinse for 1 minute Richard Allan scientific hematoxylin for 4 minutes DI water rinse for 2 minutes Richard Allan Clarifier 1 for 2.5 minutes DI water for 2 minutes Bluing Reagent for 1 minute DI water rinse for 2 minutes 95% alcohol for 1 minute Eosin Y for 2 minutes 95% alcohol for 30 seconds 2x 100% alcohol for 1 minute each 2x xylene for 1 minute each Mount and coverslip. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE ------------------------------ Message: 2 Date: Mon, 29 Feb 2016 13:12:19 +0000 From: Lester Raff MD To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Job openning in Chicago Suburbs Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B705FC0 at COLOEXCH01.uropartners.local> Content-Type: text/plain; charset="us-ascii" Our lab, a large private uropathology lab in the west Chicago suburbs, is seeking a full time day shift histologist.? Please contact me for details. Please spread the word! Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ------------------------------ Message: 3 Date: Mon, 29 Feb 2016 13:31:35 +0000 From: "Carlos Defeo" To: "Charles Riley via Histonet" Cc: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] H&E staining Message-ID: Content-Type: text/plain; format=flowed; charset=utf-8 Charles,extend time on the deparaffinization station,and even better,renew solvents at this point also. My kind regards, Carlos Defeo Histotechnologist ------------------------------ Message: 4 Date: Mon, 29 Feb 2016 14:27:25 +0000 (UTC) From: Rene J Buesa To: Charles Riley , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] H&E staining Message-ID: <1567788252.777382.1456756045073.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Usually inconsistency in H&E staining has two different sources: either the staining process itself, or the previous tissue processing sequence.Your staining protocol has a "standard" sequence but I would add an additional xylene and would increase the time in hemtoxylin to 7 minutes for you will differentiate ant way with the clarifier. Are you staining manually? If so the time in the clarifier should not be standard, the stained sections should remain in it until no more color leaches from the sections and should be as short as possible. Most likely your staining problems resides in the "clarifier" step and when the sections are of the "right" tissue type and the "right" thickness the staining is OK, but when they are either too thin or of tissues with less amounts of nuclei, the staining is faint. In any even, I strongly suggest you to change your whole protocol and eliminate xylene and alcohols in the dewaxing step, and switch to a 2% aq. sol. of dishwashing soap at 90?C (2 stations x 2 min. each) ? hot distilled water (90?C x 1 ? 45?C x 1 min) ? dist. water at room temp. which will allow you not only eliminating xylene but also ?finish the whole procedure sooner and much cheaper.Try it! Ren? ??? On Monday, February 29, 2016 6:33 AM, Charles Riley via Histonet wrote: Hello all, We've been running into an issue lately with our H&E staining. The hematoxylin and eosin are light on and off but the chromatin is consistently staining light. I have changed the time in hematoxylin to longer, used a fresh lot of both hematoxylin and our clarifier solution and the stain is not improving.? Does anyone have any suggestions? Here is our routine stain line procedure. 65 degree Celsius oven for 20 minutes 2x xylene for 5 mins 2x 100% alcohol for? 1 minute 95% alcohol for 1 minute DI water rinse for 1 minute Richard Allan scientific hematoxylin for 4 minutes DI water rinse for 2 minutes Richard Allan Clarifier 1 for 2.5 minutes DI water for 2 minutes Bluing Reagent for 1 minute DI water rinse for 2 minutes 95% alcohol for 1 minute Eosin Y for 2 minutes 95% alcohol for 30 seconds 2x 100% alcohol for 1 minute each 2x xylene for 1 minute each Mount and coverslip. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ------------------------------ Message: 5 Date: Mon, 29 Feb 2016 17:12:37 +0000 From: "FRENCH, MICHELE" To: "Histonet (histonet at lists.utsouthwestern.edu)" Subject: [Histonet] NJ Histology Meeting in June Message-ID: <0cf99f07da5f48cfa117b4dfe4ab8b85 at DM2PR26MB0030.067d.mgd.msft.net> Content-Type: text/plain; charset="us-ascii" Dear Histonet Members, I wanted to let you know that The New Jersey Society for Histotechnology is planning to host a two-day Summer Meeting June 10-11 at the Wyndham Hotel in Mount Laurel, NJ. More information will be available at the end of March, and meeting registration will start on April 1st. SPEAKERS NEEDED ASAP: We need to fill out last three time slots: (one 1.5hr session, one 3hr session and one 45minute session). VENDORS NEEDED: We only have 14 tables left in the exhibit area. Reservations are on a first-come, first-served basis. JOB LISTINGS WANTED: If you have a job opening that you would like posted on our Employment Opportunities board during the event, just let us know! Please contact Michele French by Email Michele.French at bms.com or Phone 609-818-3873. ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ------------------------------ Message: 6 Date: Mon, 29 Feb 2016 17:29:34 +0000 From: Histology To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Freeze Spray Ban Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD823433DD at vdc01.domain.local> Content-Type: text/plain; charset="us-ascii" Hi All, Just got an alert stating that the EPA is banning HFCs found in Freeze Spray effective July 20th.? Does anybody use a spray that does not contain this banned chemical?? If so, please let me know where you get it. Thanks, Mehndi Helgren Dominion Pathology Labs? "DPL" ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 147, Issue 31 ***************************************** From tony.henwood at health.nsw.gov.au Mon Feb 29 16:20:29 2016 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon, 29 Feb 2016 22:20:29 +0000 Subject: [Histonet] H&E staining In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF0147B845@SVDCMBX-MEX008.nswhealth.net> I would look at the dewaxing stage. Have you changed waxes? Try longer in xylene &/or longer in the oven. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, 29 February 2016 10:16 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] H&E staining Hello all, We've been running into an issue lately with our H&E staining. The hematoxylin and eosin are light on and off but the chromatin is consistently staining light. I have changed the time in hematoxylin to longer, used a fresh lot of both hematoxylin and our clarifier solution and the stain is not improving. Does anyone have any suggestions? Here is our routine stain line procedure. 65 degree Celsius oven for 20 minutes 2x xylene for 5 mins 2x 100% alcohol for 1 minute 95% alcohol for 1 minute DI water rinse for 1 minute Richard Allan scientific hematoxylin for 4 minutes DI water rinse for 2 minutes Richard Allan Clarifier 1 for 2.5 minutes DI water for 2 minutes Bluing Reagent for 1 minute DI water rinse for 2 minutes 95% alcohol for 1 minute Eosin Y for 2 minutes 95% alcohol for 30 seconds 2x 100% alcohol for 1 minute each 2x xylene for 1 minute each Mount and coverslip. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs Doctors Pathology Services, Dover DE _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From marktarango at gmail.com Mon Feb 29 17:39:40 2016 From: marktarango at gmail.com (Mark Tarango) Date: Mon, 29 Feb 2016 15:39:40 -0800 Subject: [Histonet] MET amplification - need help Message-ID: Hello everyone, I am working a validation for MET amplification by FISH and am having a hard time finding positive cases. Would anyone have any to share or trade? I have access to all kinds of cases from both IHC and molecular for exchange. thanks *Mark Tarango* Lead Molecular Technologist - FISH CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 PH: 206-576-6526 Fax: 206-215-5946. From wdesalvo.cac at outlook.com Mon Feb 29 17:42:32 2016 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Mon, 29 Feb 2016 16:42:32 -0700 Subject: [Histonet] automatic stainers and tissue processors Message-ID: I suggest you take the opportunity to find the instruments that fit your needs. Are going to rapid process, what types of tissue samples do you have, what do you want to improve? The histology lab is changing and you should look 5+ years down the road. I would not want you to be pigeon holed into a set of instruments. Drive quality, patient experience and flexibility. I am a wrong proponent of rapid processing, fix appropriately and do not use xylene or over process. For staining, Prisms and film are my choices. You need to find the instrumentation that works best for you. Good luck in your search, there are many options and great products to evaluate. Sent from my Windows Phone -----Original Message----- From: "Silvia Bonner via Histonet" Sent: ?2/?29/?2016 1:19 PM To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] automatic stainers and tissue processors Hello, We are looking into purchasing new automatic H&E strainers and new tissue processors. Any helpful advice? I know this may have been discussed before but I an new to histonet. Thanks for your help! Silvia Bonner, HT(ASCP) CM Histology Supervisor sbonner at pathregional.com Pathologists' Regional Laboratory 1225 Highland Ave Clarkston, WA 99403 This email may contain physician, patient and/or attorney material that is confidential or privileged for the sole use of the intended recipient. YOU ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED. If you are not the intended recipient, please notify us immediately by telephone at (208) 746-0516 or (509) 758-5576 and delete all copies. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet