From Victoria_Verno at bshsi.org Thu Dec 1 10:31:32 2016 From: Victoria_Verno at bshsi.org (Verno, Victoria R.) Date: Thu, 1 Dec 2016 16:31:32 +0000 Subject: [Histonet] HCC Control Tissue Message-ID: Hey anyone out there have any HCC control tissue? We are looking for positive liver control tissue to validate Glypican-3. Thank you for your assistance. Victoria Verno Maryview Histology Supervisor Maryview Medical Center 3636 High Street Portsmouth, VA 23707 757-398-4763 ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From sharon.celligent at gmail.com Thu Dec 1 10:58:15 2016 From: sharon.celligent at gmail.com (sharon Celligent) Date: Thu, 1 Dec 2016 11:58:15 -0500 Subject: [Histonet] Reticulin stain for poorly processed tissue Message-ID: I am working on a project and have received slides containing tissue that seem to be poorly processed. I am currently using a Chandler's Reticulin stain kit that was purchased through American MasterTech. My liver control on the test slide is working but the tissue of unknown processing is not staining. The following conditions may be the problem: poor processing, disease process, treatment of disease. Thank you, Sharon From angie at ka-recruiting.com Thu Dec 1 11:53:12 2016 From: angie at ka-recruiting.com (Angie Laparidis) Date: Thu, 1 Dec 2016 12:53:12 -0500 Subject: [Histonet] Histology Openings Nationwide Message-ID: Hi Histonet Members, I hope you are doing well. I am a Recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Histology Professionals in permanent positions across the country and I wanted to see if you or someone you know may be interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory openings across the country. Our clients typically assist with relocation expenses. Below is a list of some of the other opportunities we are currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential and free to candidates. Histology Job Opportunities (HT or HTL): AR-Little Rock-Histotech (1st shift 4:30/5 am start) FL-Tampa-HLA Supervisor IL-Chicago-Histotechnician ME-Southern/coastal-Histotech NC-Raleigh-IHC Tech NC-Raleigh-Histotech ND-Fargo-Pathologists Assistant NM-Southern-Assistant Histology Supervisor NY-Long Island-Histotech (NYS license required) NY-Long Island-Histology Supervisor MO-St Louis-Histotech (3rd shift) OH-Columbus-Histotech TN-Nashville-Histotech VA-Richmond-Histotechnologist (days) VA-Roanoke-Histotech WI-Green Bay-HT/ HTL (2nd & 3rd shifts) If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Lab positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com. Sincerely, *Angie Laparidis*Healthcare Recruiter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South, Boston, MA, 02109 W: 617.746-2744 (*please note this is a new number*) F: (617) 507-8009 Angie at ka-recruiting.com Our openings are updated daily at www.ka-recruiting.com From greg.dobbin at gmail.com Thu Dec 1 12:33:45 2016 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Thu, 1 Dec 2016 14:33:45 -0400 Subject: [Histonet] Uneven ER/PR Message-ID: Joanne, I do not think you should expect to get perfectly uniform IHC staining in breast core biopsies every time. At our hospital, we prefer to avoid doing hormone receptor stains on breast cores because they are not as "robust" as a lumpectomy or mastectomy specimen. The unevenness could be due to pre-analytic causes beyond your control (such as: are they placed in directly and immediately into formalin by the radiologist? Is your processing schedule optimized for small biopsies or is it a "one-size fits all" schedule for large and small specimens, etc). Occasionally, we will agree to an Oncology request to stain them but this is usually only done when they feel it is not going to be clinically beneficial to put the patient through a more invasive procedure (ie lumpectomy). Another disadvantage of looking at cores vs lump specimens is that you cannot see the "bigger picture". Perhaps there is heterogeneity in the intensity of the ER/PR expression throughout the tumor; something that is easier to discern when you can see the staining pattern across the entire cross-section of the tumor. I too use the Bond platform. If you are placing appropriate tissue controls on the same slide as the patient and these have the expected staining pattern and intensity, you may be fairly certain that the issue was (or is) pre-analytic in nature. If this is not your practice, then try re-staining new sections of the same case. If it is corrected, contact Leica Tech support to try to figure out what went wrong the first time. If it is not corrected, then you are back to suspecting a pre-analytic issue (assuming you are not seeing this uneven-ness in other IHC specimens). I strongly encourage use of tissue controls on every patient slide. Cheers, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From srishan at mail.holyname.org Thu Dec 1 12:43:16 2016 From: srishan at mail.holyname.org (srishan at mail.holyname.org) Date: Thu, 1 Dec 2016 13:43:16 -0500 Subject: [Histonet] (no subject) Message-ID: Since, Cell Marque H. Pylori is not available, can some one give a comparable antibody to order? Thanks in advance Nirmala Srishan Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From lblazek at digestivespecialists.com Thu Dec 1 13:08:50 2016 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Thu, 1 Dec 2016 14:08:50 -0500 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E39177AC23FD0@IBMB7Exchange.digestivespecialists.com> Try the BioCare antibody. It's excellent. -----Original Message----- From: Nirmala Srishan via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, December 01, 2016 1:43 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] (no subject) Since, Cell Marque H. Pylori is not available, can some one give a comparable antibody to order? Thanks in advance Nirmala Srishan Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From meyoshajackson at comcast.net Thu Dec 1 14:17:03 2016 From: meyoshajackson at comcast.net (WILLIAM JACKSON) Date: Thu, 1 Dec 2016 15:17:03 -0500 (EST) Subject: [Histonet] Histonet Digest, Vol 157, Issue 1 In-Reply-To: References: Message-ID: <1071181016.58663.1480623423065@connect.xfinity.com> Can someone please give me some tips in using an automatic microtome wheel, were the advance wheel keeps movivg eith forward of backward until you take your hand off of it, any little tip is useful. Meyosha Jackson HT(ASCP) > > On December 1, 2016 at 1:00 PM histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Re: Uneven ER/PR (Terri Braud) > 2. HCC Control Tissue (Verno, Victoria R.) > 3. Reticulin stain for poorly processed tissue (sharon Celligent) > 4. Histology Openings Nationwide (Angie Laparidis) > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > On November 30, 2016 at 1:38 PM Terri Braud wrote: > > My first 2 items to check whenever I have uneven IHC staining are (1) Inadequate deparaffinization, (2) bad lot of charged slides - yes this can cause terribly streaky or spotty staining, and since you are using the Bond, perhaps there was an issue with coverplate placements. > Just things to consider. Hope this helps, Terri > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > > Today's Topics: > > 1. ER/PR Uneven Staining (Joanne Clark) > Message: 2 > Date: Tue, 29 Nov 2016 21:09:17 +0000 > From: Joanne Clark > Subject: [Histonet] ER/PR Uneven Staining > I had a breast needle core today that when stained with ER and PR the staining was uneven throughout the core, even though the cancer cells were present in the entire core. The specimen had 10 hours fixation in 10% NBF. I could understand the uneven staining from inadequate fixation on large grossed in breast tissue, but 10 hours with needle core biopsies has always been more than sufficient. Does anyone have any ideas? We use Leica's ER and PR antibodies on the BOND. > Joanne Clark, BAAS, HT(ASCP)CM > Director of Histology > > On December 1, 2016 at 11:31 AM "Verno, Victoria R." wrote: > > Hey anyone out there have any HCC control tissue? We are looking for positive liver control tissue to validate Glypican-3. Thank you for your assistance. > > Victoria Verno > > Maryview Histology Supervisor > > Maryview Medical Center > > 3636 High Street > > Portsmouth, VA 23707 > > 757-398-4763 > > ________________________________________________________________________________________________________ > The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. > It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. > In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information > contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should > this communication be received in error, please notify the sender immediately either by response e-mail or by phone, > and permanently delete the original e-mail, attachment(s), and any copies. > > ________________________________________________________________________________________________________ > > On December 1, 2016 at 11:58 AM sharon Celligent wrote: > > I am working on a project and have received slides containing tissue that > seem to be poorly processed. I am currently using a Chandler's Reticulin > stain kit that was purchased through American MasterTech. My liver control > on the test slide is working but the tissue of unknown processing is not > staining. The following conditions may be the problem: poor processing, > disease process, treatment of disease. > Thank you, > Sharon > > On December 1, 2016 at 12:53 PM Angie Laparidis wrote: > > Hi Histonet Members, > > I hope you are doing well. I am a Recruiter at a highly successful and well > respected Healthcare recruiting firm. I help place Histology Professionals > in permanent positions across the country and I wanted to see if you or > someone you know may be interested in exploring other career opportunities? > We are completely free of charge to candidates and and we work on quite a > few laboratory openings across the country. Our clients typically assist > with relocation expenses. > > Below is a list of some of the other opportunities we are currently working > on. If you do not see an opening in a location in which you live or would > like to live, please send me an email me a copy of your resume and let me > know where you would be interested in a job. I will then tailor a search > for you that is completely confidential and free to candidates. > > Histology Job Opportunities (HT or HTL): > > AR-Little Rock-Histotech (1st shift 4:30/5 am start) > FL-Tampa-HLA Supervisor > IL-Chicago-Histotechnician > ME-Southern/coastal-Histotech > NC-Raleigh-IHC Tech > NC-Raleigh-Histotech > ND-Fargo-Pathologists Assistant > NM-Southern-Assistant Histology Supervisor > NY-Long Island-Histotech (NYS license required) > NY-Long Island-Histology Supervisor > MO-St Louis-Histotech (3rd shift) > OH-Columbus-Histotech > TN-Nashville-Histotech > VA-Richmond-Histotechnologist (days) > VA-Roanoke-Histotech > WI-Green Bay-HT/ HTL (2nd & 3rd shifts) > > If you're interested in learning more about these opportunities or > opportunities in a certain geographic location please reply with an updated > resume and let me know when a good time to reach you is. > > If this is not the right fit for you please let me know who you can > recommend and give me an idea of what types of positions you'd be > interested in hearing about in the future. I cover the entire US and have > am working on Lab positions at all levels. We offer a very generous > referral bonus for anyone you refer to us that we place into any position > across the country. > > To view some additional opportunities please visit our website at > www.ka-recruiting.com. > > Sincerely, > > *Angie Laparidis*Healthcare Recruiter > K.A. Recruiting, Inc. > 10 Post Office Square, 8th Floor South, Boston, MA, 02109 > W: 617.746-2744 (*please note this is a new number*) > F: (617) 507-8009 > Angie at ka-recruiting.com > > Our openings are updated daily at www.ka-recruiting.com > From MLashus at pathgroup.com Thu Dec 1 14:38:00 2016 From: MLashus at pathgroup.com (Mighnon Lashus) Date: Thu, 1 Dec 2016 20:38:00 +0000 Subject: [Histonet] Competency for Supervisors Message-ID: Did anyone have a form for the Histology Supervisor Competency? Thanks, Mighnon Lashus, HT (ASCP) Laboratory Manager PathGroup 4071 S. Access Rd, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus at pathgroup.com Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup immediately at 615-562-9255. Thank you From SteveM at mcclainlab.com Thu Dec 1 15:34:20 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Thu, 1 Dec 2016 21:34:20 +0000 Subject: [Histonet] Histonet Digest, Vol 157, Issue 1Glypican-3 Message-ID: Victoria Verno You might try normal Placenta or fetal liver from POC as non-neoplastic stain controls. That strategy may allow you enough repetitions to develop the stain until you find a case of HCC to test. Steve A. McClain, MD ********* From tony.henwood at health.nsw.gov.au Thu Dec 1 17:32:18 2016 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 1 Dec 2016 23:32:18 +0000 Subject: [Histonet] Competency for Supervisors In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF014D3D85@SVDCMBX-MEX008.nswhealth.net> Hi Mighnon, I find the so-called competency for all staff, including laboratory managers one of the stupidest items ever to be put in a standard (ie ISO 15189). All staff who perform a test need to be recorded as being competent in it, no problem with this but managers as well? How ridiculous! Why you ask? Well please forgive me in advance for the following rant: The Manager/Supervisor is responsible for instituting the test, training his/her staff in the test and importantly responsible for the quality of the test and yet His/her competency is called into question? So how does a Manager obtain a competency record for the test? 1. Ask a manager from another laboratory (?rival) to sign their competency. Could you imagine Donald Trump asking Ed McMahon to sign his competency? Since many of us sign confidentiality and privacy agreements, this will cause problems. 2. The manager could ask his staff to sign off his competency. A brilliant example of a BAD personal management decision. Why put your staff into the stressful position of deciding whether their supervisor is competent or not? If they say their supervisor is competent even though they believe they are not, then they are forced to lie possibly in order to keep their job. OR in the worst case scenario, they could hold this over the supervisor's head (and visa-versa) in order to keep their job, obtain favours or monetary gain. I often wonder whether the authors of this item in the standard were complete idiots. They probably never had to face the stresses of managing a laboratory and especially valuable scientific staff. Absolutely stupid!! We stepped up our competency assessment by evolving it into a Continuing Education exercise, including questions and scenarios for my staff to consider and hopefully learn from. I was then asked why I had not done the same tests. Well I wrote the tests so one would assume that I would know the answers - a little self-serving? Unfortunately I could not test myself when I was attempting my driving licence, I would have passed the first time!! Enough of a rant - please advise whether I am driving up the wrong tree! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Mighnon Lashus via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 2 December 2016 7:38 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Competency for Supervisors Did anyone have a form for the Histology Supervisor Competency? Thanks, Mighnon Lashus, HT (ASCP) Laboratory Manager PathGroup 4071 S. Access Rd, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus at pathgroup.com Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup immediately at 615-562-9255. Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From allanvv at gmail.com Sun Dec 4 01:23:58 2016 From: allanvv at gmail.com (Allan Wang) Date: Sun, 4 Dec 2016 02:23:58 -0500 Subject: [Histonet] Some questions about dehydration and clearing Message-ID: Hello, I've been doing some histology work but without formal training. Just some questions about protocol: 1) Is it okay to dehydrate directly into 100% alcohol or does that cause tissue shrinkage? Is starting at 95% good enough? I'm trying to reduce the number of stations needed. 2) I've once been told that after dehydration, I should wait for all the alcohol to dry off before clearing with xylene. Does that make sense, since I couldn't find this step in any protocols I've seen. Or would this mess up morphology? Sometimes I see some inconsistent appearance of cells after IHC staining, not sure if it's due to issues with this dehydration step/clearing or if it's the change in antibody dilution that's causing it. I have a comparison image here, where I think something went wrong with Slide 2. Both IHC was performed with an autostainer. http://i.imgur.com/y0q4hb7.jpg Thanks, Allan Wang From j.rowaihi at alborglaboratories.com Sun Dec 4 02:24:31 2016 From: j.rowaihi at alborglaboratories.com (Jamal Rwaihi) Date: Sun, 4 Dec 2016 11:24:31 +0300 Subject: [Histonet] unsatisfactory Thin prep Message-ID: <001601d24e07$d7c84690$8758d3b0$@alborglaboratories.com> Dear colleagues I appreciate if some can share me their statistics in unsatisfactory ThinPrep and what it's the best solution to reduce the amount of unsatisfactory results. Best Regards, Jamal M. Al Rowaihi Al Borg Medical Laboratories I Anatomic Pathology Supervisor I Headquarters, Jeddah, KSA I Phone: +966 12 670 0099 I Mobile +966 503629832 www.alborglaboratories.com From gagnone at KGH.KARI.NET Mon Dec 5 13:59:52 2016 From: gagnone at KGH.KARI.NET (Gagnon, Eric) Date: Mon, 5 Dec 2016 19:59:52 +0000 Subject: [Histonet] ER/PR Uneven Staining In-Reply-To: <57ae34a9ab5a465384e9fa4a582db780@E15MADAG-D06N04.sh11.lan> References: <57ae34a9ab5a465384e9fa4a582db780@E15MADAG-D06N04.sh11.lan> Message-ID: <5F06C3AD0B27264CA20CFA986C87882E01BB670048@EXCHANGEPV1.KGH.ON.CA> As Greg has already mentioned, placing control sections on each patient slide will assist in troubleshooting your staining issue. Joanne, I don't believe you mentioned it in your initial email, but did you attempt a repeat and if so, did the results replicate on the repeat? Occasionally there may be a mixing issue or other physical issue with the covertile that inhibits complete reaction across the slide. Eric Gagnon MLT Histology Laboratory Kingston General Hospital, Kingston, Ontario, Canada ________________________________________ From: Joanne Clark via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 29, 2016 4:09 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] ER/PR Uneven Staining I had a breast needle core today that when stained with ER and PR the staining was uneven throughout the core, even though the cancer cells were present in the entire core. The specimen had 10 hours fixation in 10% NBF. I could understand the uneven staining from inadequate fixation on large grossed in breast tissue, but 10 hours with needle core biopsies has always been more than sufficient. Does anyone have any ideas? We use Leica's ER and PR antibodies on the BOND. Joanne Clark, BAAS, HT(ASCP)CM Director of Histology P. (575) 622-5600 C. (575) 317-6403 F. (575) 622-3720 TF. (800) 753-7284 pcnm.com Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From allanvv at gmail.com Mon Dec 5 14:42:03 2016 From: allanvv at gmail.com (Allan Wang) Date: Mon, 5 Dec 2016 15:42:03 -0500 Subject: [Histonet] RTU antibodies with 1+ year expiration date Message-ID: Hello list, Thanks for your responses to my previous dehydration question. I pretty much got conflicting responses about going straight to 100% alcohol, but I think I will just start them out in 80% alcohol to be safe. Something that's been bugging me: Every source states that diluted antibodies shouldn't be kept for more than a few weeks. Yet Ventana dispensers of pre-diluted antibodies have expiration dates of 1 - 1.5 years, plus they sit out at room temperature for 5 hours at a time whenever they're used. Ventana provides user-fillable dispensers, but if we dilute antibodies ourselves and can't use them for more than a few weeks, it would be kind of pointless for low-volume needs. Their specs say: "The antibody is diluted in 0.08 M PBS with 3% carrier protein and 0.05% ProClin 300, a preservative." Isn't this pretty much the same stuff that antibody diluent is composed of, except with sodium azide instead of ProClin 300 (I think they're equivalent). I'm already planning on doing some long-term testing of my own over a year with HER-2 and Ki67, but hopefully someone can provide some insight. Thanks, Allan Wang From houv2 at uw.edu Mon Dec 5 14:58:22 2016 From: houv2 at uw.edu (Vivian Hou) Date: Mon, 5 Dec 2016 12:58:22 -0800 Subject: [Histonet] Weak Hematoxylin Staining? Message-ID: Dear all, We are doing some immunofluorescent stains that we want to pair with H&E slides. We are using fresh frozen sections for both (unfixed tissue, human diseased coronary arteries) but I am seeing very weak hematoxylin staining to the point where I have to dial back the eosin (3 min hematoxylin, 1 dip eosin). I am using Gills 2 (newly purchased), I know its not necessary to filter it but we have been just in case using Whatman no. 5 filters. No differentiation due to the weak staining right now. I would appreciate any thoughts on why the staining might be so weak, we do a great deal of processing and imaging on the arteries (about 12 hrs @ 25C) prior to histo and I wonder if this has cause the tissue to decay? Thank you for your time, V -- ----------------------------------------------------------------------------------------- V Hou Bioengineering | Human Photonics Laboratory e: houv2 at uw.edu | c: 206-999-3708 From blayjorge at gmail.com Mon Dec 5 15:22:35 2016 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Mon, 5 Dec 2016 16:22:35 -0500 Subject: [Histonet] What are science faculty members doing when evaluating anti-science papers in science classes? Message-ID: What are science faculty members doing when evaluating anti-science papers in science classes? Dear Colleagues: To encourage civilized face to face or digital discussions, in my science classes, I like to ask questions about topics some people find controversial (e.g. age of Earth, age of human lineage, global climate change, etc.). What are science faculty members doing when evaluating anti-science papers in science classes? I would be interested in knowing whether there are differences between what people do in different countries. If you have any constructive response, please feel free to send it directly to me at blayjorge at gmail.com Apologies for potential duplicate emails. Gratefully, Jorge blaypublishers.com From tony.henwood at health.nsw.gov.au Mon Dec 5 16:44:23 2016 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon, 5 Dec 2016 22:44:23 +0000 Subject: [Histonet] RTU antibodies with 1+ year expiration date In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF014D46EF@SVDCMBX-MEX008.nswhealth.net> Hi Alan, Not every source states that diluted antibodies only last for a few weeks, in fact my experience as well as those of the commercial suppliers (see their data sheets) indicates otherwise, for example, I just re-validated an antibody (CD45RO) that was prepared 8 years ago (but unfortunately rarely used) and it worked very well, in fact we had to re-titre it since it seems our detection system has improved its sensitivity (we have a Bond 3). Since it is not routinely used (last time was at least 4 years ago) we have retired it. The following is an extract from the handout of a workshop we gave on an Immunohistochemistry Validation workshop we gave this year: Expired Antibodies Usually when a new concentrated antibody is received it will have an expiry date of around 2 years from receipt, but most of us will find that we can continue to use antibodies well past this expiry date. So where are we at? Recent discussions on Histonet reveal: ? At least five labs admit to routinely discarding expired antibodies. ? Why don?t companies extend expiry dates? ? Why not freeze aliquots of antibody to extend the shelf life? ? If controls continue to stain appropriately, then why can?t expiry be extended? An expiration date labelled on a vial merely reflects the time span tested by the manufacturer during which performance of a reagent has remained stable. It does not imply per se, that the reagent will not function properly beyond the indicated date. In fact, because it is unrealistic for a manufacturer to test an antibody for aging during decades before putting it on the market, most expiration spans for primary antibodies are set arbitrarily within 6 to 24 months (Balaton et al 1999). Dr Hadi Yaziji explains: Vendors do stability testing on their antibodies, where they leave them at room temperature for an extended time (one month, six months, etc.), or they do what's referred to as 'accelerated' testing, where they put them in a microwave which is supposed to accelerate the degradation of the protein. That's usually how they decide to assign an expiration date. Dr Hadi Yaziji also suggests that if you aliquot the antibody before its expiration date and store it in the -20oC freezer, the clock is in effect "frozen" too. For example, if there are 6 months left before the antibody expires and you freeze the aliquots for 5 years, then if you thaw one of the frozen aliquots, the thawed aliquot will still be good for 6 months. He also recommends that you make sure you don't thaw and re-freeze the aliquot. Unfortunately, some antibody data sheets state ?do not freeze?. One Histotechnologist reported that their CAP inspector told them it was not acceptable, so had to allow him to watch them throw all the frozen aliquots away. Both Dr Terry Marshall (UK) and Dr Richard Cartun suggest that if the antibody continues to stain control sections appropriately, with no loss of sensitivity and no increase in non-specific staining then its use should be continued. If positive control samples are deemed unsatisfactory, even if the antibody is within the manufacturer?s printed expiration date, evaluation of the clinical specimen is aborted and the test deemed invalid. The quality of the primary antibody is therefore not based on an expiration date, but rather on its performance on a case-by-case basis with appropriate positive and negative control samples (Savage & DeYoung 2010). Savage & DeYoung (2010) had previously examined the staining patterns of 26 recently acquired primary antibodies and their expired counterparts. Two reviewers examined sequential sections of formalin-fixed, paraffin-embedded tissue samples for staining intensity and percentage of positivity. Appropriate positive and negative control studies were performed. Of the 26 antibodies, 20 exhibited no difference in percentage of positivity or staining intensity. Of the remaining 6, 3 showed better performance with the expired cohort and 3 with non-expired antibodies. However, no antibody staining characteristics varied by more than 1 step and in no case was positive staining lost after antibody expiration. Negligible differences exist in immunostaining between outdated and current antibodies. They concluded that exemption for primary antibodies from existing regulations would conserve resources without adversely impacting patient care. Balaton et al (1999) compared 55 antibodies that had expired 6 to 134 months previously and found there was no significant difference observed in the specificity, sensitivity, and pattern of staining between old versus new antibodies. Recently Maria Argentieri et al (2013) investigated whether the shelf-life of diagnostic antibodies was longer than the expiry date on the label. They had four independent laboratories test a small number of diagnostic antibodies kept at +4?C for 12?26 years, and found them to work perfectly on routine histology sections. Monoclonal antibodies originally supplied as culture supernatants or as ascites (neat or diluted), of all isotypes, as well as all of the polyclonal antibodies, produced satisfactory staining irrespective of their age. Notable exceptions were ammonium-precipitated, IgM or conjugated antibodies. Drachenberg et al (2001) suggest that instead of a mechanical adherence to the manufacturers? recommendations, a rational quality assurance system would be preferable. References: Argentieri, M. C., Pilla, D., Vanzati, A., Lonardi, S., Facchetti, F., Doglioni, C., ... & Cattoretti, G. (2013). Antibodies are forever: a study using 12?26?year?old expired antibodies. Histopathology, 63(6), 869-876. Balaton, A. J., Drachenberg, C. B., Rucker, C., Vaury, P., & Papadimitriou, J. C. (1999). Satisfactory performance of primary antibodies beyond manufacturers' recommended expiration dates. Applied Immunohistochemistry & Molecular Morphology, 7(3), 221. Drachenberg, C. B., Papadimitriou, J. C., Balaton, A. J., & Vaury, P. (2001). The total test approach to standardization of immunohistochemistry. Archives of pathology & laboratory medicine, 125(4), 471-471. Savage, E. C., & DeYoung, B. R. (2010). Antibody Expiration in the Context of Resource Limitation What Is the Evidence Basis?. American journal of clinical pathology, 134(1), 60-64. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Allan Wang via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, 6 December 2016 7:42 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] RTU antibodies with 1+ year expiration date Hello list, Thanks for your responses to my previous dehydration question. I pretty much got conflicting responses about going straight to 100% alcohol, but I think I will just start them out in 80% alcohol to be safe. Something that's been bugging me: Every source states that diluted antibodies shouldn't be kept for more than a few weeks. Yet Ventana dispensers of pre-diluted antibodies have expiration dates of 1 - 1.5 years, plus they sit out at room temperature for 5 hours at a time whenever they're used. Ventana provides user-fillable dispensers, but if we dilute antibodies ourselves and can't use them for more than a few weeks, it would be kind of pointless for low-volume needs. Their specs say: "The antibody is diluted in 0.08 M PBS with 3% carrier protein and 0.05% ProClin 300, a preservative." Isn't this pretty much the same stuff that antibody diluent is composed of, except with sodium azide instead of ProClin 300 (I think they're equivalent). I'm already planning on doing some long-term testing of my own over a year with HER-2 and Ki67, but hopefully someone can provide some insight. Thanks, Allan Wang _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From tony.henwood at health.nsw.gov.au Mon Dec 5 16:52:54 2016 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon, 5 Dec 2016 22:52:54 +0000 Subject: [Histonet] Weak Hematoxylin Staining? In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF014D4710@SVDCMBX-MEX008.nswhealth.net> Vivian, After cutting the frozen sections, how are you fixing them? If they are air-dried then they will tend to give a washed out nuclear stain. I would suggest immediate fixation of the sections in either methanol or to make even a brighter H&E: 1% acetic acid in ethanol (1ml glacial acetic acid + 99ml absolute ethanol), about 1 minute should suffice for both fixations. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Vivian Hou via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, 6 December 2016 7:58 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Weak Hematoxylin Staining? Dear all, We are doing some immunofluorescent stains that we want to pair with H&E slides. We are using fresh frozen sections for both (unfixed tissue, human diseased coronary arteries) but I am seeing very weak hematoxylin staining to the point where I have to dial back the eosin (3 min hematoxylin, 1 dip eosin). I am using Gills 2 (newly purchased), I know its not necessary to filter it but we have been just in case using Whatman no. 5 filters. No differentiation due to the weak staining right now. I would appreciate any thoughts on why the staining might be so weak, we do a great deal of processing and imaging on the arteries (about 12 hrs @ 25C) prior to histo and I wonder if this has cause the tissue to decay? Thank you for your time, V -- ----------------------------------------------------------------------------------------- V Hou Bioengineering | Human Photonics Laboratory e: houv2 at uw.edu | c: 206-999-3708 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From houv2 at uw.edu Mon Dec 5 17:06:04 2016 From: houv2 at uw.edu (Vivian Hou) Date: Mon, 5 Dec 2016 15:06:04 -0800 Subject: [Histonet] Weak Hematoxylin Staining? In-Reply-To: <0237449DE79DBC45B686AB82CDCD16FF014D4710@SVDCMBX-MEX008.nswhealth.net> References: <0237449DE79DBC45B686AB82CDCD16FF014D4710@SVDCMBX-MEX008.nswhealth.net> Message-ID: Hi Tony, I have not been fixing them but will try the 1% acetic in ethanol and the methanol solutions, thank you for the suggestion! Best, Vivian On Mon, Dec 5, 2016 at 2:52 PM, Tony Henwood (SCHN) < tony.henwood at health.nsw.gov.au> wrote: > Vivian, > > After cutting the frozen sections, how are you fixing them? > > If they are air-dried then they will tend to give a washed out nuclear > stain. > I would suggest immediate fixation of the sections in either methanol or > to make even a brighter H&E: 1% acetic acid in ethanol (1ml glacial acetic > acid + 99ml absolute ethanol), about 1 minute should suffice for both > fixations. > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: Vivian Hou via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, 6 December 2016 7:58 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Weak Hematoxylin Staining? > > Dear all, > > We are doing some immunofluorescent stains that we want to pair with H&E > slides. We are using fresh frozen sections for both (unfixed tissue, human > diseased coronary arteries) but I am seeing very weak hematoxylin staining > to the point where I have to dial back the eosin (3 min hematoxylin, 1 dip > eosin). > > > I am using Gills 2 (newly purchased), I know its not necessary to filter > it but we have been just in case using Whatman no. 5 filters. No > differentiation due to the weak staining right now. > > > I would appreciate any thoughts on why the staining might be so weak, we > do a great deal of processing and imaging on the arteries (about 12 hrs @ > 25C) prior to histo and I wonder if this has cause the tissue to decay? > > > Thank you for your time, > V > > -- > ------------------------------------------------------------ > ----------------------------- > V Hou > Bioengineering | Human Photonics Laboratory > e: houv2 at uw.edu | c: 206-999-3708 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain > confidential information. If you are not the intended recipient, please > delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and > are not necessarily the views of NSW Health or any of its entities. > > -- ----------------------------------------------------------------------------------------- V Hou Bioengineering | Human Photonics Laboratory e: houv2 at uw.edu | c: 206-999-3708 From Timothy.Morken at ucsf.edu Mon Dec 5 17:37:47 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 5 Dec 2016 23:37:47 +0000 Subject: [Histonet] RTU antibodies with 1+ year expiration date In-Reply-To: <0237449DE79DBC45B686AB82CDCD16FF014D46EF@SVDCMBX-MEX008.nswhealth.net> References: <0237449DE79DBC45B686AB82CDCD16FF014D46EF@SVDCMBX-MEX008.nswhealth.net> Message-ID: <761E2B5697F795489C8710BCC72141FF88E2CEF6@ex07.net.ucsf.edu> To the specific question of if lab-made predilutes will last longer than two weeks. Yes they can. But be sure you are using reasonably sterile technique so they don't become contaminated. Then validate how long they last. I know of one lab in the "old" days of manual staining that kept their antibodies in Coplin jars in the fridge and used them for many weeks. They just pulled out a particular antibody jar and added slides when that antibody was ordered. It made bulk manual staining much easier. I agree with all Tony writes below. One observation from experience working for a vendor of antibodies... While vendors test for reasonable shelf life, the fairly standard 2-year expiration date is implemented more to limit a vendors liability than indicate actual shelf life. We tested ours for up to 4 months in a 60 C oven and I rarely saw one fail. Indeed, some, as Tony noted, became better with age and heat! While those of us who have worked in IHC for many, many years know that most modern antibodies are very stable, and, if frozen, good for decades, the various inspection agencies are very focused on expiration dates. Part of that may be ease of enforcement (not having to look though hundreds of pages of revalidations) and part not trusting labs to do good revalidations. And many labs would much rather not revalidate since it takes work to do and if you have many antibodies, it could be a lot of work (we have over 250 on our menu). Much easier to get new ones. We don't revalidate, but we also make every effort to only have on hand that which will not expire before used. We have very few that have so few orders that the vial lasts 2 years. And we still dilute a fair number that are not available as predilutes from our instrument vendor. And thanks for all the references! Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Tony Henwood (SCHN) via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, December 05, 2016 2:44 PM To: Allan Wang Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] RTU antibodies with 1+ year expiration date Hi Alan, Not every source states that diluted antibodies only last for a few weeks, in fact my experience as well as those of the commercial suppliers (see their data sheets) indicates otherwise, for example, I just re-validated an antibody (CD45RO) that was prepared 8 years ago (but unfortunately rarely used) and it worked very well, in fact we had to re-titre it since it seems our detection system has improved its sensitivity (we have a Bond 3). Since it is not routinely used (last time was at least 4 years ago) we have retired it. The following is an extract from the handout of a workshop we gave on an Immunohistochemistry Validation workshop we gave this year: Expired Antibodies Usually when a new concentrated antibody is received it will have an expiry date of around 2 years from receipt, but most of us will find that we can continue to use antibodies well past this expiry date. So where are we at? Recent discussions on Histonet reveal: ? At least five labs admit to routinely discarding expired antibodies. ? Why don?t companies extend expiry dates? ? Why not freeze aliquots of antibody to extend the shelf life? ? If controls continue to stain appropriately, then why can?t expiry be extended? An expiration date labelled on a vial merely reflects the time span tested by the manufacturer during which performance of a reagent has remained stable. It does not imply per se, that the reagent will not function properly beyond the indicated date. In fact, because it is unrealistic for a manufacturer to test an antibody for aging during decades before putting it on the market, most expiration spans for primary antibodies are set arbitrarily within 6 to 24 months (Balaton et al 1999). Dr Hadi Yaziji explains: Vendors do stability testing on their antibodies, where they leave them at room temperature for an extended time (one month, six months, etc.), or they do what's referred to as 'accelerated' testing, where they put them in a microwave which is supposed to accelerate the degradation of the protein. That's usually how they decide to assign an expiration date. Dr Hadi Yaziji also suggests that if you aliquot the antibody before its expiration date and store it in the -20oC freezer, the clock is in effect "frozen" too. For example, if there are 6 months left before the antibody expires and you freeze the aliquots for 5 years, then if you thaw one of the frozen aliquots, the thawed aliquot will still be good for 6 months. He also recommends that you make sure you don't thaw and re-freeze the aliquot. Unfortunately, some antibody data sheets state ?do not freeze?. One Histotechnologist reported that their CAP inspector told them it was not acceptable, so had to allow him to watch them throw all the frozen aliquots away. Both Dr Terry Marshall (UK) and Dr Richard Cartun suggest that if the antibody continues to stain control sections appropriately, with no loss of sensitivity and no increase in non-specific staining then its use should be continued. If positive control samples are deemed unsatisfactory, even if the antibody is within the manufacturer?s printed expiration date, evaluation of the clinical specimen is aborted and the test deemed invalid. The quality of the primary antibody is therefore not based on an expiration date, but rather on its performance on a case-by-case basis with appropriate positive and negative control samples (Savage & DeYoung 2010). Savage & DeYoung (2010) had previously examined the staining patterns of 26 recently acquired primary antibodies and their expired counterparts. Two reviewers examined sequential sections of formalin-fixed, paraffin-embedded tissue samples for staining intensity and percentage of positivity. Appropriate positive and negative control studies were performed. Of the 26 antibodies, 20 exhibited no difference in percentage of positivity or staining intensity. Of the remaining 6, 3 showed better performance with the expired cohort and 3 with non-expired antibodies. However, no antibody staining characteristics varied by more than 1 step and in no case was positive staining lost after antibody expiration. Negligible differences exist in immunostaining between outdated and current antibodies. They concluded that exemption for primary antibodies from existing regulations would conserve resources without adversely impacting patient care. Balaton et al (1999) compared 55 antibodies that had expired 6 to 134 months previously and found there was no significant difference observed in the specificity, sensitivity, and pattern of staining between old versus new antibodies. Recently Maria Argentieri et al (2013) investigated whether the shelf-life of diagnostic antibodies was longer than the expiry date on the label. They had four independent laboratories test a small number of diagnostic antibodies kept at +4?C for 12?26 years, and found them to work perfectly on routine histology sections. Monoclonal antibodies originally supplied as culture supernatants or as ascites (neat or diluted), of all isotypes, as well as all of the polyclonal antibodies, produced satisfactory staining irrespective of their age. Notable exceptions were ammonium-precipitated, IgM or conjugated antibodies. Drachenberg et al (2001) suggest that instead of a mechanical adherence to the manufacturers? recommendations, a rational quality assurance system would be preferable. References: Argentieri, M. C., Pilla, D., Vanzati, A., Lonardi, S., Facchetti, F., Doglioni, C., ... & Cattoretti, G. (2013). Antibodies are forever: a study using 12?26?year?old expired antibodies. Histopathology, 63(6), 869-876. Balaton, A. J., Drachenberg, C. B., Rucker, C., Vaury, P., & Papadimitriou, J. C. (1999). Satisfactory performance of primary antibodies beyond manufacturers' recommended expiration dates. Applied Immunohistochemistry & Molecular Morphology, 7(3), 221. Drachenberg, C. B., Papadimitriou, J. C., Balaton, A. J., & Vaury, P. (2001). The total test approach to standardization of immunohistochemistry. Archives of pathology & laboratory medicine, 125(4), 471-471. Savage, E. C., & DeYoung, B. R. (2010). Antibody Expiration in the Context of Resource Limitation What Is the Evidence Basis?. American journal of clinical pathology, 134(1), 60-64. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Allan Wang via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, 6 December 2016 7:42 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] RTU antibodies with 1+ year expiration date Hello list, Thanks for your responses to my previous dehydration question. I pretty much got conflicting responses about going straight to 100% alcohol, but I think I will just start them out in 80% alcohol to be safe. Something that's been bugging me: Every source states that diluted antibodies shouldn't be kept for more than a few weeks. Yet Ventana dispensers of pre-diluted antibodies have expiration dates of 1 - 1.5 years, plus they sit out at room temperature for 5 hours at a time whenever they're used. Ventana provides user-fillable dispensers, but if we dilute antibodies ourselves and can't use them for more than a few weeks, it would be kind of pointless for low-volume needs. Their specs say: "The antibody is diluted in 0.08 M PBS with 3% carrier protein and 0.05% ProClin 300, a preservative." Isn't this pretty much the same stuff that antibody diluent is composed of, except with sodium azide instead of ProClin 300 (I think they're equivalent). I'm already planning on doing some long-term testing of my own over a year with HER-2 and Ki67, but hopefully someone can provide some insight. Thanks, Allan Wang _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pat.Patterson at propath.com Mon Dec 5 23:41:35 2016 From: Pat.Patterson at propath.com (Pat Patterson) Date: Tue, 6 Dec 2016 05:41:35 +0000 Subject: [Histonet] Dallas Texas Lab Positions Message-ID: <6DCB8B92D0138244B56CE8EACE0D458D3EB304D6@Mail.propathlab.com> ProPath, a progressive, CAP accredited, high volume, pathology practice, located in Dallas, Texas, is seeking candidates for the following positions: HISTOLOGY TECHNOLOGIST/TECHNICIAN Responsibilities include embedding tissue specimens, microtomy of paraffin-embedded tissue, operation of automated stainer and cover slipper, equipment maintenance, and record retention. HT & HTL (ASCP) registered or eligible. Experience preferred. Hours are 7:00 a.m. to 3:30 p.m. or 6:00 p.m. to 2:30 a.m. IHC HISTOLOGY TECHNOLOGIST/TECHNICIAN Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. A minimum of 4 years Histology experience with at least 1-2 years immunohistochemistry, immunofluorescence or in situ hybridization experience. Working knowledge of IHC theory required and hands on IHC performance desired. Hours for this position are 5:00 p.m. to 1:30 a.m. GROSSING HISTOTECHNICIAN Responsibilities include performing gross dictation and dissect low to moderate complexity specimens received in the laboratory for microscopic diagnosis. You will work in Histology and will be responsible for embedding tissue specimens, microtomy of paraffin-embedded tissue, special stains, frozen sections, operation of automated stainer and coverslipper, equipment maintenance and record retention. Bachelors' degree in a laboratory science or medical laboratory technology plus HT/ HTL (ASCP) certification required and 3 years' experience in grossing and histology. Hours for this position are 11:00 p.m. to 7:30 a.m. For more details and to apply, please visit www.propath.com ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! EEO/AA-M/F/disability/protected veteran status Pat Patterson, HTL(ASCP) Manager, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From tbraud at holyredeemer.com Tue Dec 6 12:50:35 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 6 Dec 2016 18:50:35 +0000 Subject: [Histonet] RTU antibodies with 1+ year expiration date Message-ID: <48E053DDF6CE074DB6A7414BA05403F8113CA2@HRHEX03-HOS.holyredeemer.local> Just a word to the wise: Although I certainly appreciate the well-referenced, sound science behind Tony Henwood's advice, as he also pointed out, if your lab is CAP or CLIA inspected, the regulations are quite specific that expired reagents cannot be used for patient work. I agree that it is a waste, and we all want to be responsible and resourceful, but better to throw away and reorder expired reagents than a phase II deficiency. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 2. RTU antibodies with 1+ year expiration date (Allan Wang) 5. Re: RTU antibodies with 1+ year expiration date (Tony Henwood (SCHN)) 8. Re: RTU antibodies with 1+ year expiration date (Morken, Timothy) Message: 2 Date: Mon, 5 Dec 2016 15:42:03 -0500 From: Allan Wang Subject: [Histonet] RTU antibodies with 1+ year expiration date Hello list, Thanks for your responses to my previous dehydration question. I pretty much got conflicting responses about going straight to 100% alcohol, but I think I will just start them out in 80% alcohol to be safe. Something that's been bugging me: Every source states that diluted antibodies shouldn't be kept for more than a few weeks. Yet Ventana dispensers of pre-diluted antibodies have expiration dates of 1 - 1.5 years, plus they sit out at room temperature for 5 hours at a time whenever they're used. Ventana provides user-fillable dispensers, but if we dilute antibodies ourselves and can't use them for more than a few weeks, it would be kind of pointless for low-volume needs. Their specs say: "The antibody is diluted in 0.08 M PBS with 3% carrier protein and 0.05% ProClin 300, a preservative." Isn't this pretty much the same stuff that antibody diluent is composed of, except with sodium azide instead of ProClin 300 (I think they're equivalent). I'm already planning on doing some long-term testing of my own over a year with HER-2 and Ki67, but hopefully someone can provide some insight. Thanks, Allan Wang -------------------------------------------------------- Message: 5 Date: Mon, 5 Dec 2016 22:44:23 +0000 From: "Tony Henwood (SCHN)" To: Allan Wang > Subject: Re: [Histonet] RTU antibodies with 1+ year expiration date Hi Alan, Not every source states that diluted antibodies only last for a few weeks, in fact my experience as well as those of the commercial suppliers (see their data sheets) indicates otherwise, for example, I just re-validated an antibody (CD45RO) that was prepared 8 years ago (but unfortunately rarely used) and it worked very well, in fact we had to re-titre it since it seems our detection system has improved its sensitivity (we have a Bond 3). Since it is not routinely used (last time was at least 4 years ago) we have retired it. The following is an extract from the handout of a workshop we gave on an Immunohistochemistry Validation workshop we gave this year: Expired Antibodies Usually when a new concentrated antibody is received it will have an expiry date of around 2 years from receipt, but most of us will find that we can continue to use antibodies well past this expiry date. So where are we at? Recent discussions on Histonet reveal: ? At least five labs admit to routinely discarding expired antibodies. ? Why don?t companies extend expiry dates? ? Why not freeze aliquots of antibody to extend the shelf life? ? If controls continue to stain appropriately, then why can?t expiry be extended? An expiration date labelled on a vial merely reflects the time span tested by the manufacturer during which performance of a reagent has remained stable. It does not imply per se, that the reagent will not function properly beyond the indicated date. In fact, because it is unrealistic for a manufacturer to test an antibody for aging during decades before putting it on the market, most expiration spans for primary antibodies are set arbitrarily within 6 to 24 months (Balaton et al 1999). Dr Hadi Yaziji explains: Vendors do stability testing on their antibodies, where they leave them at room temperature for an extended time (one month, six months, etc.), or they do what's referred to as 'accelerated' testing, where they put them in a microwave which is supposed to accelerate the degradation of the protein. That's usually how they decide to assign an expiration date. Dr Hadi Yaziji also suggests that if you aliquot the antibody before its expiration date and store it in the -20oC freezer, the clock is in effect "frozen" too. For example, if there are 6 months left before the antibody expires and you freeze the aliquots for 5 years, then if you thaw one of the frozen aliquots, the thawed aliquot will still be good for 6 months. He also recommends that you make sure you don't thaw and re-freeze the aliquot. Unfortunately, some antibody data sheets state ?do not freeze?. One Histotechnologist reported that their CAP inspector told them it was not acceptable, so had to allow him to watch them throw all the frozen aliquots away. Both Dr Terry Marshall (UK) and Dr Richard Cartun suggest that if the antibody continues to stain control sections appropriately, with no loss of sensitivity and no increase in non-specific staining then its use should be continued. If positive control samples are deemed unsatisfactory, even if the antibody is within the manufacturer?s printed expiration date, evaluation of the clinical specimen is aborted and the test deemed invalid. The quality of the primary antibody is therefore not based on an expiration date, but rather on its performance on a case-by-case basis with appropriate positive and negative control samples (Savage & DeYoung 2010). Savage & DeYoung (2010) had previously examined the staining patterns of 26 recently acquired primary antibodies and their expired counterparts. Two reviewers examined sequential sections of formalin-fixed, paraffin-embedded tissue samples for staining intensity and percentage of positivity. Appropriate positive and negative control studies were performed. Of the 26 antibodies, 20 exhibited no difference in percentage of positivity or staining intensity. Of the remaining 6, 3 showed better performance with the expired cohort and 3 with non-expired antibodies. However, no antibody staining characteristics varied by more than 1 step and in no case was positive staining lost after antibody expiration. Negligible differences exist in immunostaining between outdated and current antibodies. They concluded that exemption for primary antibodies from existing regulations would conserve resources without adversely impacting patient care. Balaton et al (1999) compared 55 antibodies that had expired 6 to 134 months previously and found there was no significant difference observed in the specificity, sensitivity, and pattern of staining between old versus new antibodies. Recently Maria Argentieri et al (2013) investigated whether the shelf-life of diagnostic antibodies was longer than the expiry date on the label. They had four independent laboratories test a small number of diagnostic antibodies kept at +4?C for 12?26 years, and found them to work perfectly on routine histology sections. Monoclonal antibodies originally supplied as culture supernatants or as ascites (neat or diluted), of all isotypes, as well as all of the polyclonal antibodies, produced satisfactory staining irrespective of their age. Notable exceptions were ammonium-precipitated, IgM or conjugated antibodies. Drachenberg et al (2001) suggest that instead of a mechanical adherence to the manufacturers? recommendations, a rational quality assurance system would be preferable. References: Argentieri, M. C., Pilla, D., Vanzati, A., Lonardi, S., Facchetti, F., Doglioni, C., ... & Cattoretti, G. (2013). Antibodies are forever: a study using 12?26?year?old expired antibodies. Histopathology, 63(6), 869-876. Balaton, A. J., Drachenberg, C. B., Rucker, C., Vaury, P., & Papadimitriou, J. C. (1999). Satisfactory performance of primary antibodies beyond manufacturers' recommended expiration dates. Applied Immunohistochemistry & Molecular Morphology, 7(3), 221. Drachenberg, C. B., Papadimitriou, J. C., Balaton, A. J., & Vaury, P. (2001). The total test approach to standardization of immunohistochemistry. Archives of pathology & laboratory medicine, 125(4), 471-471. Savage, E. C., & DeYoung, B. R. (2010). Antibody Expiration in the Context of Resource Limitation What Is the Evidence Basis?. American journal of clinical pathology, 134(1), 60-64. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA From relia1 at earthlink.net Wed Dec 7 07:51:44 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 7 Dec 2016 08:51:44 -0500 Subject: [Histonet] A favor for a friend... PRN Mohs tech needed in St. Petersburg, FL Message-ID: <005801d25091$0c623b50$2526b1f0$@earthlink.net> Hi Histonetters! Happy Hump Day!! I wanted to drop a quick post for a friend of mine. They are in need of a PRN Mohs tech in St. Petersburg, FL. If you would like more information shoot me an email and I will put you in touch with them. Have a great day!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Happy Holidays! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From relia1 at earthlink.net Wed Dec 7 10:20:54 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 7 Dec 2016 11:20:54 -0500 Subject: [Histonet] RELIA Histology Careers Bulletin 12-7-2016 Grab a cup of Cocoa and have a look at some exciting opportunities. Message-ID: <009801d250a5$e2752680$a75f7380$@earthlink.net> Hi Histonetters! Happy Holidays!! While a job change is the last thing on most people's minds this time of year; May I take a minute of your time to ask; Is a job change the next logical step in your career? This is a special bulletin for you. Some of my best clients are expanding and as a result some fantastic opportunities are open right now. I wanted to touch base and give you a heads up. Your next opportunity might be just around the corner and I might have it for you. If you are looking for a position right now please contact me right away!! In addition to these opportunities I have new positions coming in DAILY!! If you aren't looking right away but want to let me know what would make a perfect job for you; so that I could keep an eye out that would be great too. To do that, just shoot me an email at relia1 at earthlink.net or call me toll free at 866-607-3542 or call/text 407-353-5070. Here Is A List Of My Current Opportunities: Histology Supervisor- Santa Ana, CA Histology Supervisor - Seattle, WA Lead Histology Tech - Cheyenne, WY Dermpath Histotech - St. Louis, MO Grossing Histotech - Austin, TX IHC Specialist - Modesto, CA Histotechnician - Modesto, CA Histotech - Manitowoc, WI My clients offer competitive salaries, great benefits and in most cases relocation assistance and or sign on bonuses. **While all of these clients would LOVE to hire right away; they are also willing to start the process with a phone interview now and continue the process after the holidays if that would be better for you! Remember, I offer a 250.00 dollar referral fee if I place someone you refer to me. I really appreciate you taking time out of your busy day to read my e-mail Thanks Again! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From taylor at prometheushealthcare.com Wed Dec 7 10:25:06 2016 From: taylor at prometheushealthcare.com (Taylor Rinaldi) Date: Wed, 7 Dec 2016 11:25:06 -0500 Subject: [Histonet] **Histology Job Opportunities** Message-ID: <001301d250a6$78edcf90$6ac96eb0$@prometheushealthcare.com> Hi there! My name is Taylor Rinaldi, Recruiting Manager at Prometheus healthcare. My team and I specialize specifically in laboratory recruiting nationwide, working directly with lab managers from different hospitals and reference laboratories across the US . We are currently recruiting for multiple Histotechnologist and Histotechnician opportunities. All the opportunities are fulltime, and permanent. ASCP certification is preferred but not required for all. If you or any of your colleagues have been considering a new position in the Histology field, please don't hesitate to reach out to me directly for immediate referral and submittal to some of the top hospitals and labs nationwide. Current states: Nashville, Tennessee Sacramento, California New York, New York Atlanta, Georgia Dallas, Texas Thank you all in advance, Taylor Rinaldi Recruiting Manager Prometheus Healthcare Office 866-857-1434 Taylor at prometheushealthcare.com From tkngflght at yahoo.com Wed Dec 7 12:22:30 2016 From: tkngflght at yahoo.com (Cheryl) Date: Wed, 7 Dec 2016 18:22:30 +0000 (UTC) Subject: [Histonet] PRN or part time in Houston -- ASAP References: <104365841.1611422.1481134950907.ref@mail.yahoo.com> Message-ID: <104365841.1611422.1481134950907@mail.yahoo.com> Hi Histonetters- Happy holidays! Looking for PRN/Part Time for routine histology in Houston (good processing, new tomes --clean biopsy work) in Houston. ?The shift will be one or a combo of two shirst: ? nights starting around 12M and really early AM (4-5A). We can be flexible depending on your situation. If you're grossing qualified, so much the better!! This is a FAST RESPONSE need -- want to support our staff for time off going into the holidays so if you need a little extra for the family-- call or email (include resume if you're comfortable-- it stays private until you say go!) Holler!! Cell 281.883.7704?Cheryl Kerry, HT(ASCP) Full Staff Inc. ? admin at fullstaff.org?800.756.3309 Phone & Fax https://www.facebook.com/TheHistologyCompany/ From SteveM at mcclainlab.com Wed Dec 7 13:55:51 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Wed, 7 Dec 2016 19:55:51 +0000 Subject: [Histonet] Histonet Digest, Vol 157, Issue 5 outdated gold In-Reply-To: References: Message-ID: <456280EB-1755-46EF-8550-3C147D2720D0@mcclainlab.com> Cheers! We were dinged on the last inspection for having outdated reagents near the human testing. We have a gold standard in the lab, specifically a Queen Victoria 0.25 ounce gold coin minted in 1870. It appears to be past expiration, yet still attractive and untarnished. Any suggestions for proper disposal of outdated gold will be appreciated. Steve A. McClain, MD > On Dec 7, 2016, at 13:23, "histonet-request at lists.utsouthwestern.edu" wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: RTU antibodies with 1+ year expiration date (Terri Braud) > 2. A favor for a friend... PRN Mohs tech needed in St. > Petersburg, FL (Pam Barker) > 3. RELIA Histology Careers Bulletin 12-7-2016 Grab a cup of > Cocoa and have a look at some exciting opportunities. (Pam Barker) > 4. **Histology Job Opportunities** (Taylor Rinaldi) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 6 Dec 2016 18:50:35 +0000 > From: "Terri Braud" > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: Re: [Histonet] RTU antibodies with 1+ year expiration date > Message-ID: > <48E053DDF6CE074DB6A7414BA05403F8113CA2 at HRHEX03-HOS.holyredeemer.local> > > Content-Type: text/plain; charset="us-ascii" > > Just a word to the wise: Although I certainly appreciate the well-referenced, sound science behind Tony Henwood's advice, as he also pointed out, if your lab is CAP or CLIA inspected, the regulations are quite specific that expired reagents cannot be used for patient work. I agree that it is a waste, and we all want to be responsible and resourceful, but better to throw away and reorder expired reagents than a phase II deficiency. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > 2. RTU antibodies with 1+ year expiration date (Allan Wang) > 5. Re: RTU antibodies with 1+ year expiration date > (Tony Henwood (SCHN)) > 8. Re: RTU antibodies with 1+ year expiration date (Morken, Timothy) > > Message: 2 > Date: Mon, 5 Dec 2016 15:42:03 -0500 > From: Allan Wang > Subject: [Histonet] RTU antibodies with 1+ year expiration date > Hello list, > Thanks for your responses to my previous dehydration question. I pretty much got conflicting responses about going straight to 100% alcohol, but I think I will just start them out in 80% alcohol to be safe. > Something that's been bugging me: Every source states that diluted antibodies shouldn't be kept for more than a few weeks. Yet Ventana dispensers of pre-diluted antibodies have expiration dates of 1 - 1.5 years, plus they sit out at room temperature for 5 hours at a time whenever they're used. Ventana provides user-fillable dispensers, but if we dilute antibodies ourselves and can't use them for more than a few weeks, it would be kind of pointless for low-volume needs. > Their specs say: "The antibody is diluted in 0.08 M PBS with 3% carrier protein and 0.05% ProClin 300, a preservative." > Isn't this pretty much the same stuff that antibody diluent is composed of, except with sodium azide instead of ProClin 300 (I think they're equivalent). > I'm already planning on doing some long-term testing of my own over a year with HER-2 and Ki67, but hopefully someone can provide some insight. > Thanks, > Allan Wang > -------------------------------------------------------- > > Message: 5 > Date: Mon, 5 Dec 2016 22:44:23 +0000 > From: "Tony Henwood (SCHN)" > To: Allan Wang > > Subject: Re: [Histonet] RTU antibodies with 1+ year expiration date > Hi Alan, > Not every source states that diluted antibodies only last for a few weeks, in fact my experience as well as those of the commercial suppliers (see their data sheets) indicates otherwise, for example, I just re-validated an antibody (CD45RO) that was prepared 8 years ago (but unfortunately rarely used) and it worked very well, in fact we had to re-titre it since it seems our detection system has improved its sensitivity (we have a Bond 3). Since it is not routinely used (last time was at least 4 years ago) we have retired it. > The following is an extract from the handout of a workshop we gave on an Immunohistochemistry Validation workshop we gave this year: > Expired Antibodies > Usually when a new concentrated antibody is received it will have an expiry date of around 2 years from receipt, but most of us will find that we can continue to use antibodies well past this expiry date. So where are we at? > Recent discussions on Histonet reveal: > ? At least five labs admit to routinely discarding expired antibodies. > ? Why don?t companies extend expiry dates? > ? Why not freeze aliquots of antibody to extend the shelf life? > ? If controls continue to stain appropriately, then why can?t expiry be extended? > An expiration date labelled on a vial merely reflects the time span tested by the manufacturer during which performance of a reagent has remained stable. It does not imply per se, that the reagent will not function properly beyond the indicated date. In fact, because it is unrealistic for a manufacturer to test an antibody for aging during decades before putting it on the market, most expiration spans for primary antibodies are set arbitrarily within 6 to 24 months (Balaton et al 1999). > > Dr Hadi Yaziji explains: Vendors do stability testing on their antibodies, where they leave them at room temperature for an extended time (one month, six months, etc.), or they do what's referred to as 'accelerated' testing, where they put them in a microwave which is supposed to accelerate the degradation of the protein. That's usually how they decide to assign an expiration date. > > Dr Hadi Yaziji also suggests that if you aliquot the antibody before its expiration date and store it in the -20oC freezer, the clock is in effect "frozen" too. For example, if there are 6 months left before the antibody expires and you freeze the aliquots for 5 years, then if you thaw one of the frozen aliquots, the thawed aliquot will still be good for 6 months. He also recommends that you make sure you don't thaw and re-freeze the aliquot. Unfortunately, some antibody data sheets state ?do not freeze?. One Histotechnologist reported that their CAP inspector told them it was not acceptable, so had to allow him to watch them throw all the frozen aliquots away. > > Both Dr Terry Marshall (UK) and Dr Richard Cartun suggest that if the antibody continues to stain control sections appropriately, with no loss of sensitivity and no increase in non-specific staining then its use should be continued. If positive control samples are deemed unsatisfactory, even if the antibody is within the manufacturer?s printed expiration date, evaluation of the clinical specimen is aborted and the test deemed invalid. The quality of the primary antibody is therefore not based on an expiration date, but rather on its performance on a case-by-case basis with appropriate positive and negative control samples (Savage & DeYoung 2010). > > Savage & DeYoung (2010) had previously examined the staining patterns of 26 recently acquired primary antibodies and their expired counterparts. Two reviewers examined sequential sections of formalin-fixed, paraffin-embedded tissue samples for staining intensity and percentage of positivity. Appropriate positive and negative control studies were performed. Of the 26 antibodies, 20 exhibited no difference in percentage of positivity or staining intensity. Of the remaining 6, 3 showed better performance with the expired cohort and 3 with non-expired antibodies. However, no antibody staining characteristics varied by more than 1 step and in no case was positive staining lost after antibody expiration. Negligible differences exist in immunostaining between outdated and current antibodies. They concluded that exemption for primary antibodies from existing regulations would conserve resources without adversely impacting patient care. > > Balaton et al (1999) compared 55 antibodies that had expired 6 to 134 months previously and found there was no significant difference observed in the specificity, sensitivity, and pattern of staining between old versus new antibodies. > Recently Maria Argentieri et al (2013) investigated whether the shelf-life of diagnostic antibodies was longer than the expiry date on the label. They had four independent laboratories test a small number of diagnostic antibodies kept at +4?C for 12?26 years, and found them to work perfectly on routine histology sections. Monoclonal antibodies originally supplied as culture supernatants or as ascites (neat or diluted), of all isotypes, as well as all of the polyclonal antibodies, produced satisfactory staining irrespective of their age. Notable exceptions were ammonium-precipitated, IgM or conjugated antibodies. > > Drachenberg et al (2001) suggest that instead of a mechanical adherence to the manufacturers? recommendations, a rational quality assurance system would be preferable. > > References: > Argentieri, M. C., Pilla, D., Vanzati, A., Lonardi, S., Facchetti, F., Doglioni, C., ... & Cattoretti, G. (2013). Antibodies are forever: a study using 12?26?year?old expired antibodies. Histopathology, 63(6), 869-876. > Balaton, A. J., Drachenberg, C. B., Rucker, C., Vaury, P., & Papadimitriou, J. C. (1999). Satisfactory performance of primary antibodies beyond manufacturers' recommended expiration dates. Applied Immunohistochemistry & Molecular Morphology, 7(3), 221. > Drachenberg, C. B., Papadimitriou, J. C., Balaton, A. J., & Vaury, P. (2001). The total test approach to standardization of immunohistochemistry. Archives of pathology & laboratory medicine, 125(4), 471-471. > Savage, E. C., & DeYoung, B. R. (2010). Antibody Expiration in the Context of Resource Limitation What Is the Evidence Basis?. American journal of clinical pathology, 134(1), 60-64. > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children?s Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > > > ------------------------------ > > Message: 2 > Date: Wed, 7 Dec 2016 08:51:44 -0500 > From: "Pam Barker" > To: "Histonet" > Subject: [Histonet] A favor for a friend... PRN Mohs tech needed in > St. Petersburg, FL > Message-ID: <005801d25091$0c623b50$2526b1f0$@earthlink.net> > Content-Type: text/plain; charset="us-ascii" > > Hi Histonetters! > Happy Hump Day!! I wanted to drop a quick post for a friend of mine. They > are in need of a PRN Mohs tech in St. Petersburg, FL. > If you would like more information shoot me an email and I will put you in > touch with them. > Have a great day!! > > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Happy Holidays! > > Thank You! > Pam M. Barker > > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1 at earthlink.net > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > www.facebook.com /PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > > > > > > > > ------------------------------ > > Message: 3 > Date: Wed, 7 Dec 2016 11:20:54 -0500 > From: "Pam Barker" > To: "Histonet" > Subject: [Histonet] RELIA Histology Careers Bulletin 12-7-2016 Grab a > cup of Cocoa and have a look at some exciting opportunities. > Message-ID: <009801d250a5$e2752680$a75f7380$@earthlink.net> > Content-Type: text/plain; charset="us-ascii" > > Hi Histonetters! > Happy Holidays!! > While a job change is the last thing on most people's minds this time of > year; > May I take a minute of your time to ask; > Is a job change the next logical step in your career? > > This is a special bulletin for you. > Some of my best clients are expanding and as a result some fantastic > opportunities are open right now. > I wanted to touch base and give you a heads up. > Your next opportunity might be just around the corner > and > I might have it for you. > If you are looking for a position right now please contact me right away!! > In addition to these opportunities I have new positions coming in DAILY!! > If you aren't looking right away but want to let me know what would make a > perfect job for you; > so that I could keep an eye out that would be great too. > To do that, just shoot me an email at relia1 at earthlink.net or > call me toll free at 866-607-3542 or call/text 407-353-5070. > > Here Is A List Of My Current Opportunities: > Histology Supervisor- Santa Ana, CA > Histology Supervisor - Seattle, WA > Lead Histology Tech - Cheyenne, WY > Dermpath Histotech - St. Louis, MO > Grossing Histotech - Austin, TX > IHC Specialist - Modesto, CA > Histotechnician - Modesto, CA > Histotech - Manitowoc, WI > > My clients offer competitive salaries, great benefits and in most cases > relocation assistance and or sign on bonuses. > **While all of these clients would LOVE to hire right away; they are also > willing to start the process with a phone interview now and continue the > process after the holidays if that would be better for you! > > Remember, I offer a 250.00 dollar referral fee if I place someone you refer > to me. > > I really appreciate you taking time out of your busy day to read my e-mail > Thanks Again! > > > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > Pam M. Barker > > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1 at earthlink.net > www.facebook.com /PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > > > > ------------------------------ > > Message: 4 > Date: Wed, 7 Dec 2016 11:25:06 -0500 > From: "Taylor Rinaldi" > To: > Subject: [Histonet] **Histology Job Opportunities** > Message-ID: <001301d250a6$78edcf90$6ac96eb0$@prometheushealthcare.com> > Content-Type: text/plain; charset="us-ascii" > > Hi there! > > > > My name is Taylor Rinaldi, Recruiting Manager at Prometheus healthcare. My > team and I specialize specifically in laboratory recruiting nationwide, > working directly with lab managers from different hospitals and reference > laboratories across the US . We are currently recruiting for multiple > Histotechnologist and Histotechnician opportunities. All the opportunities > are fulltime, and permanent. ASCP certification is preferred but not > required for all. > > > > If you or any of your colleagues have been considering a new position in the > Histology field, please don't hesitate to reach out to me directly for > immediate referral and submittal to some of the top hospitals and labs > nationwide. > > > > Current states: > > Nashville, Tennessee > > Sacramento, California > > New York, New York > > Atlanta, Georgia > > Dallas, Texas > > > > Thank you all in advance, > > > > Taylor Rinaldi > > Recruiting Manager > > Prometheus Healthcare > > Office 866-857-1434 > > Taylor at prometheushealthcare.com > > > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 157, Issue 5 > **************************************** From allanvv at gmail.com Wed Dec 7 14:38:50 2016 From: allanvv at gmail.com (Allan Wang) Date: Wed, 7 Dec 2016 15:38:50 -0500 Subject: [Histonet] Histotech opening in Rockville, MD. Immediate Message-ID: Hello list, I'm looking for a histotech with 2-4 years of experience, starting immediately. Position is at a small business which is a biorepository for cancer researchers worldwide. Job responsibilities: 1. Section, mount and stain very valuable tissue blocks. No tissue processing for now. 2. Perform IHC on automated platform (Ventana Discovery XT) - training will be provided 3. Opportunity to train in other lab roles such as NGS library preparation and DNA sequencing Must have a few years of sectioning experience. Some IHC experience desirable. Certification optional. Please email me and I will contact qualified applicants immediately. Thanks, Allan Wang From akemiat3377 at gmail.com Wed Dec 7 19:42:16 2016 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Wed, 7 Dec 2016 17:42:16 -0800 Subject: [Histonet] New Hire for My Lab Message-ID: <8B34D477-2305-4CEF-995B-14725F23B128@gmail.com> Attention West Coast Histology Students and Graduates! If you will be graduating, and are eligible for certification, or are recently graduated and certified; our GI Endoscopy laboratory in Beautiful Monterey, CA is expanding, and we are going to hire another histologist. We have a formalin free environment, with new Microwave Hybrid Tissue processing Technology. This is a great opportunity for the right person. WILL TRAIN a knowledgeable, willing candidate. Duties to include (but not limited to): accessioning, data entry into AP Easy LIS System, grossing, embedding, microtomy, and general histology *must be self motivated, reliable and a team player. Preferred that you meet CLIA grossing regulations. Monday through Friday with no weekends. We offer full benefits and 401 K. Salary is negotiable. If you are interested, or if you know of someone, contact me and I will give you more details. akemiat3377 at gmail.comStudents! If you will be graduating and are eligible for certification, or are certified histologists; our GI Laboratory in Beautiful Monterey, CA is expanding, and we are going to hire another histologist. Preferred that you meet CLIA grossing regulations. This is a great opportunity for the right person. WILL TRAIN a knowledgeable, willing candidate. Duties to include (but not limited to): accessioning, data entry into AP Easy LIS System, grossing, embedding, microtomy, and general histology *must be self motivated, reliable and a team player. Monday through Friday with no weekends. We offer full benefits and 401 K. Salary is negotiable. If you are interested, or if you know of someone, contact me and I will give you more details. akemiat3377 at gmail.com Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com Tele: (831) 375-3577 X117 From akemiat3377 at gmail.com Thu Dec 8 18:27:08 2016 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Thu, 8 Dec 2016 16:27:08 -0800 Subject: [Histonet] histology opening Message-ID: <10BA8F87-B583-4116-A483-63950F1C9FC2@gmail.com> Hope this is acceptable. I am a Pathology Manager and am looking for a histologist to work for us. Here is my ad; A Great Opportunity for West Coast Histology Students and Graduates! If you will be graduating, and are eligible for certification, or are recently graduated and certified; our GI endoscopy pathology laboratory, located in a private practice Gastroenterology medical group in beautiful Monterey, CA is expanding to hire another histologist. Our lab has a formalin-free environment with new microwave hybrid tissue processing technology. We will train a knowledgeable and willing candidate. Duties to include (but not limited to): accessioning, data entry into AP Easy LIS System, grossing, embedding, microtomy, limited manual special stains, general histology and histology related clerical duties. The right candidate is reliable, self-motivated, articulate and well versed with anatomy identifications. It is preferred that you meet CLIA grossing regulations. Full Time Days, Monday through Friday with no weekends. We offer full benefits including medical, dental and vision insurance, paid holidays, vacation and sick accrual and a profit sharing 401K plan. Salary is negotiable. If you are interested, or if you know of someone, contact me and I will give you more details. Akemi Allison, BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 Work: (831) 375-3577 X117 W.Email: aallison at montereygi.com H. Email: akemiat3377 at gmail.com From relia1 at earthlink.net Fri Dec 9 09:17:20 2016 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 9 Dec 2016 10:17:20 -0500 Subject: [Histonet] Here's a Real GEM from a few years back. From Ashley Troutman - The Twelve Days of Christmas for Histotechs! Message-ID: <006201d2522f$5875a050$0960e0f0$@earthlink.net> Hi Histonetters! Here is a gem found on the histonet in 12/2013from Ashley Troutman the 12 days of Christmas for Histotechs Enjoy Histopeeps: Hello all, I hope the holidays are treating everyone well. (Especially in light of us having the most hazardous job in the country...) ... Here's some fun for the season: The Twelve Days of Histo TWELVE Medical Directors ELEVEN logs-a-printing TEN residents reading NINE frozen sections EIGHT administrators SEVEN stainers beeping SIX techs complaining FIVE I...H...Cs.... :) FOUR calling docs THREE recuts TWO special stains And a block with an H and EEEEE... Please feel free to sing along. Merry Christmas in Histoland! Ashley Troutman BS, MBA, HT(ASCP) QIHC Thanks-Pam Right Place, Right Time, Right Move with RELIA! Happy Holidays! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Richard.Cartun at hhchealth.org Fri Dec 9 10:33:15 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 9 Dec 2016 16:33:15 +0000 Subject: [Histonet] Hard copy pathology reports Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E953D3C99@HHCEXCHMB03.hhcsystem.org> Since we are now using EPIC we will no longer be providing (mailing) hard-copy pathology reports to our clients effective January 1, 2017. Our healthcare providers now have access to all lab results and pathology reports in EPIC. Physicians can also be set-up for auto-faxing or can call client services for a faxed copy of the report if needed. The issue we are having is with the providers that are "CC:'ed" on pathology requisitions. Many of these individuals are not in our system or don't have access to EPIC. Has anyone else experienced this issue and, if so, what have you done to make sure all care-givers get their pathology reports? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From victor_tobias at comcast.net Fri Dec 9 10:39:10 2016 From: victor_tobias at comcast.net (victor_tobias at comcast.net) Date: Fri, 9 Dec 2016 08:39:10 -0800 Subject: [Histonet] Hard copy pathology reports In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E953D3C99@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E953D3C99@HHCEXCHMB03.hhcsystem.org> Message-ID: Richard, All internal docs get their reports via EPIC or ORCA unless they specify otherwise. All external clients get a fax or print copy. We have not heard of any problems where there is more than one internal provider not getting a report. Victor Sent from Mail for Windows 10 From: Cartun, Richard via Histonet Sent: Friday, December 9, 2016 8:34 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Hard copy pathology reports Since we are now using EPIC we will no longer be providing (mailing) hard-copy pathology reports to our clients effective January 1, 2017. Our healthcare providers now have access to all lab results and pathology reports in EPIC. Physicians can also be set-up for auto-faxing or can call client services for a faxed copy of the report if needed. The issue we are having is with the providers that are "CC:'ed" on pathology requisitions. Many of these individuals are not in our system or don't have access to EPIC. Has anyone else experienced this issue and, if so, what have you done to make sure all care-givers get their pathology reports? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff at uropartners.com Fri Dec 9 10:54:47 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 9 Dec 2016 16:54:47 +0000 Subject: [Histonet] Hard copy pathology reports In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E953D3C99@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E953D3C99@HHCEXCHMB03.hhcsystem.org> Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF111213A4@COLOEXCH01.uropartners.local> We don't use epic, but run into the same issue with our system which is integrated into the groups EHR. We fax hard copies to outside providers. To do this we try to maintain an up-to-date list of fax numbers, but it is obviously difficult. Our ordering doctor's offices are supposed to inform us of fax number changes from there referrers, but that doesn't always happen. Lester Raff, MD UroPartners Laboratory Latest blog: http://www.chicagonow.com/downsize-maybe/2016/12/popped-but-not-profiled-a-lucky-day-police-experience/ -----Original Message----- From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, December 09, 2016 10:33 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Hard copy pathology reports Since we are now using EPIC we will no longer be providing (mailing) hard-copy pathology reports to our clients effective January 1, 2017. Our healthcare providers now have access to all lab results and pathology reports in EPIC. Physicians can also be set-up for auto-faxing or can call client services for a faxed copy of the report if needed. The issue we are having is with the providers that are "CC:'ed" on pathology requisitions. Many of these individuals are not in our system or don't have access to EPIC. Has anyone else experienced this issue and, if so, what have you done to make sure all care-givers get their pathology reports? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Fri Dec 9 10:59:55 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 9 Dec 2016 16:59:55 +0000 Subject: [Histonet] Outdated Gold Message-ID: <48E053DDF6CE074DB6A7414BA05403F81145BC@HRHEX03-HOS.holyredeemer.local> To clarify, the mint date on the Queen Victoria 0.25 ounce gold piece is the "Manufacture Date" not the expiration date. Also, since it clearly does not have an expiration date, regulations state that an expiration date must be assigned to the coin. Then, the effectiveness of the gold piece in chemical procedures must be validated through testing with known controls on a minimum of an annual basis. The results of that testing must be kept for the lifetime of the coin. If you chose instead, to have the gold piece disposed, you must know the content of the gold and any component parts. Is it 14k? 18k? A reputable hazardous waste facility will sample the coin for its component parts (at an additional charge, of course) then will charge for disposal of the gold piece and the other metals it may contain. The cost of the disposal is usually calculated by weight and type hazardous materials. After disposal, the company should send you a waste manifest that (at least in the US) must be kept on file for 50 years. (Secretly, I would just bury it in my backyard, a common technique in the U.S.) Terri L. Braud, HT(ASCP) ------------------------------ Message: 2 Date: Wed, 7 Dec 2016 19:55:51 +0000 From: Steve McClain Subject: Re: [Histonet] Histonet Digest, Vol 157, Issue 5 outdated gold Cheers! We were dinged on the last inspection for having outdated reagents near the human testing. We have a gold standard in the lab, specifically a Queen Victoria 0.25 ounce gold coin minted in 1870. It appears to be past expiration, yet still attractive and untarnished. Any suggestions for proper disposal of outdated gold will be appreciated. Steve A. McClain, MD From tkngflght at yahoo.com Fri Dec 9 11:30:35 2016 From: tkngflght at yahoo.com (Cheryl) Date: Fri, 9 Dec 2016 17:30:35 +0000 (UTC) Subject: [Histonet] short biopsy process for a Tissue Tek VIP - References: <1005303688.1255120.1481304635264.ref@mail.yahoo.com> Message-ID: <1005303688.1255120.1481304635264@mail.yahoo.com> Help?? Anyone have a good 3-hour process on a VIP they can share? ?We're using StatLab reagents and all small biopsy work-- VIP-5 smaller version.? Would love to start from a proven process that's been tweaked to perfection ?than start from scratch... thank you!!?Cheryl Kerry, HT(ASCP) ? tkngflght at yahoo.com From SteveM at mcclainlab.com Fri Dec 9 16:37:39 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Fri, 9 Dec 2016 22:37:39 +0000 Subject: [Histonet] Rapid processing With Conventional Processors and conventional (old school) reagents Message-ID: I posted this previously. We have done this more than 2400 times with a number of processors. It can be done if you pay close attention to processing only FIXED tissues, use fresh reagents and lay out the cassettes not more than two high. Steve 631 361 4000 Station 1 50% 10 min Station 2 70% 10 min Station 3 95% 5 min Station 4 95% 10 min Station 5 100% 5 min Station 6 100% 10 min Station 7 100% 20 min Station 8 Xylene 5 min Station 9 Xylene 10 min Station 10 Xylene 20 Min Station 11 Paraffin 5 min Station 12 Paraffin 10 min Station 13 Paraffin 20 Min (VIP5 Station 14 Paraffin 20min) Total Time to complete ~3hours I posted our 7 year experience on this topic previously on histonet. Remember, this machine (K3000, VIP5) was not designed specifically for this purpose, but it can be made to work admirably. IOW Short processing on VIP is unreliable UNLESS you are willing to accept or control certain conditions, 1) only process well-fixed tissue 2) take formalin off this processor, making 14 changes beginning in 50%. If you want to keep station 1 formalin and standardize fixation with time X in formalin at temp Y, that may work. I prefer to change the tissues to 50% alcohol at the grossing bench so that all tissues are not only fixed, but rinsed in alcohol and started processing at the growing bench. This simplifies processing to dehydration, clearing and paraffin infiltration. Simple is reliable and short processing cycles on this machine leave little room for error. 2) Program heat to 50C in all alcohols and xylene. Mixing pressure and vacuum yes yes yes 4) Keep all solutions fresh, rotate after 200- 400 blocks. 5) Lay blocks out not more than 2 cassettes back to back, max 100 for VIP5. 4 hour is reliable for tissues up to 3mm thickness such as punch biopsy. 2 hour is reliable for up to 1.5 mm tissue thickness such as core biopsies and shave biopsies of skin. Method of processor validation for short cycle in machine previously validated in your lab. To validate create your evaluation form and find a set of cases with sufficient tissue small tissue to divide into 2 equal blocks. This can (and will) be done on clinical cases if you are careful. Print duplicate sets of cassettes. Mark one half of case 1 yellow and one half blue (tissues will be processed separately, 1 regular and one short cycle and then recombined into 1 final block at embedding). We held the short processed tissue cassettes in formalin and timed the short cycle to end when the regular program ends. Then the two blocks are recombined or embedded together so that direct comparisons/measures can be made on one H&E and one on your most common IHC for that tissue. If the H&E and IHC both work, chances are everything else will also. Modify your regular validation form or Create a form for scoring shrinkage, measured thickness and length, staining reactions, shrinkage, holes and flaws, have your path score. photo graph yellow and blue tissues under H&E and IHC. IF you do observe shrinkage or other undesirable artifact, be certain that your tissues are completely fixed at grossing and repeat. If they are comparable in the oculars and to the camera, then show your work and voila, there is your validation. Steve A. McClain, MD > Message: 7 Date: Fri, 9 Dec 2016 17:30:35 +0000 (UTC) From: Cheryl To: Histonet Subject: [Histonet] short biopsy process for a Tissue Tek VIP - Message-ID: <1005303688.1255120.1481304635264 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Help?? Anyone have a good 3-hour process on a VIP they can share? ?We're using StatLab reagents and all small biopsy work-- VIP-5 smaller version.? Would love to start from a proven process that's been tweaked to perfection ?than start from scratch... thank you!!?Cheryl Kerry, HT(ASCP) ? tkngflght at yahoo.com From SteveM at mcclainlab.com Sat Dec 10 07:10:17 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Sat, 10 Dec 2016 13:10:17 +0000 Subject: [Histonet] Rapid processing With Conventional Processors and conventional (old school) reagents In-Reply-To: References: Message-ID: <5F301482-EBA2-406E-8E0D-D897484A6BB6@mcclainlab.com> This can be modified to run shorter as a 2:20hr program by subtracting time from the last alcohol Last xylene and Last paraffin Station 1 50% 10 min Station 2 70% 10 min Station 3 95% 5 min Station 4 95% 10 min Station 5 100% 5 min Station 6 100% 10 min Station 7 100% 10 min Station 8 Xylene 5 min Station 9 Xylene 10 min Station 10 Xylene 10 Min Station 11 Paraffin 5 min Station 12 Paraffin 10 min Station 13 Paraffin 10 Min (VIP5 Station 14 Paraffin 10min) Total Time to complete ~2:20hours Steve A. McClain, MD > On Dec 9, 2016, at 17:37, Steve McClain wrote: > > Station 1 50% 10 min > Station 2 70% 10 min > Station 3 95% 5 min > Station 4 95% 10 min > Station 5 100% 5 min > Station 6 100% 10 min > Station 7 100% 20 min > Station 8 Xylene 5 min > Station 9 Xylene 10 min > Station 10 Xylene 20 Min > Station 11 Paraffin 5 min > Station 12 Paraffin 10 min > Station 13 Paraffin 20 Min (VIP5 Station 14 Paraffin 20min) > Total Time to complete ~3hours > > > From lauriediane811 at yahoo.com Tue Dec 13 06:31:58 2016 From: lauriediane811 at yahoo.com (Laurie Best) Date: Tue, 13 Dec 2016 07:31:58 -0500 Subject: [Histonet] Employment Message-ID: <7A8943CD-FF5C-43F6-999B-92A1358143ED@yahoo.com> Two open Histology positions East Setauket, NY 6am-2:30pm 12am- 8:30am NY license required Please contact off line for details. lauriediane811 at yahoo.com From angie at ka-recruiting.com Tue Dec 13 14:07:55 2016 From: angie at ka-recruiting.com (Angie Laparidis) Date: Tue, 13 Dec 2016 15:07:55 -0500 Subject: [Histonet] Little Rock Hitotech Message-ID: Dear Histonet users, I wanted to reach out to share a brand new DAY SHIFT histotech position in the Little Rock, AK area! This client is looking for someone with a clinical background setting to step into their permanent, Full-time, histotechnologist opportunity. Located in a fantastic area, they are also a world-renown academic center that can further transcend your background! Please send over an updated resume to angie at ka-recruiting.com if you are interested in hearing more information. Thank you! Sincerely, *Angie Laparidis*Healthcare Recruiter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South, Boston, MA, 02109 W: 617.746-2744 (*please note this is a new number*) F: (617) 507-8009 Angie at ka-recruiting.com Our openings are updated daily at www.ka-recruiting.com From angie at ka-recruiting.com Tue Dec 13 14:54:43 2016 From: angie at ka-recruiting.com (Angie Laparidis) Date: Tue, 13 Dec 2016 15:54:43 -0500 Subject: [Histonet] AR NOT AK adjustment Message-ID: Dear Histonet users, I wanted to reach out to share a brand new DAY SHIFT histotech position in the Little Rock, AR area! This client is looking for someone with a clinical background setting to step into their permanent, Full-time, histotechnologist opportunity. Located in a fantastic area, they are also a world-renown academic center that can further transcend your background! Please send over an updated resume to angie at ka-recruiting.com if you are interested in hearing more information. Thank you! Sincerely, *Angie Laparidis*Healthcare Recruiter K.A. Recruiting, Inc. 10 Post Office Square, 8th Floor South, Boston, MA, 02109 W: 617.746-2744 (*please note this is a new number*) F: (617) 507-8009 Angie at ka-recruiting.com Our openings are updated daily at www.ka-recruiting.com From LBUSTAMANTE at cvm.tamu.edu Tue Dec 13 14:59:29 2016 From: LBUSTAMANTE at cvm.tamu.edu (Bustamante, Lin) Date: Tue, 13 Dec 2016 20:59:29 +0000 Subject: [Histonet] MEDITE Microtome Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC9019F689950@CVMMB02.cvm.tamu.edu> We are looking into purchasing new microtome for our laboratory. At NSH I saw this company and we are interesting to know the opinion of current users. We are looking into the fully automated one. Thank you very much. Lin. Lin S. Bustamante, BSc, HT(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone (979)845-3177 Fax (979)458-3499 From aaditza1 at gmail.com Tue Dec 13 15:47:00 2016 From: aaditza1 at gmail.com (Aditza) Date: Tue, 13 Dec 2016 15:47:00 -0600 Subject: [Histonet] Thermo Excelsior AS processor? Message-ID: Dear Histotechs!! Does any of you use the Thermo Excelsior AS processor? Do you like it? Is the tissue looking good? Reagents are not wasted? What don't you like about it? I'm planning to buy a refurbished one and need advice!! Thank you!! Sincerely, Adriana Rosca BS, HTL (ASCP) MS "...what we say and how we say it, is still the foundation of behavior change." From cls71877 at gmail.com Tue Dec 13 16:05:05 2016 From: cls71877 at gmail.com (Cristi Rigazio) Date: Tue, 13 Dec 2016 17:05:05 -0500 Subject: [Histonet] Peloris Processing Schedules Message-ID: Hi Histoland, I am currently establishing new processing schedules for the Peloris processor and wondered if anyone had a schedule for large tissue they would be willing to share? Also, maybe one for excisional/lumpectomy/mastectomy cases? I have been testing one for large tissue that is approximately 4 hours, but I think a little longer in formalin may be necessary. Anyone willing to share their experiences with the Peloris is greatly appreciated. Happy Holidays, Cristi From bcooper at chla.usc.edu Tue Dec 13 16:50:22 2016 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Tue, 13 Dec 2016 22:50:22 +0000 Subject: [Histonet] BRAF V600E Message-ID: Good Afternoon Histonet! It's been a few years since I posted this question initially; thought I'd give it a try again. Is anyone successfully utilizing Roche's BRAF V600e (VE1) (or Spring Bioscience's) antibody on a Leica Bond Max? If you are, I'd really appreciate hearing how you did it. This antibody is quite expensive, and we don't particularly want to burn through massive amounts of money trying to reinvent the wheel. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From Klaus.Kruttwig at leibniz-inm.de Wed Dec 14 08:44:57 2016 From: Klaus.Kruttwig at leibniz-inm.de (Kruttwig, Klaus) Date: Wed, 14 Dec 2016 14:44:57 +0000 Subject: [Histonet] histological processing of silicone Message-ID: <205F5EB48253894F8CBF08EBFA3C7A9199E5CF@inmex10.intern.inm-gmbh.de> Dear colleagues, has anyone experience with embedding and sectioning of tissue attached to silicone, e.g. PDMS? I tried cryosectioning as well as sectioning of paraffin containing thin sheets of silicone with-or without tissue. Both methods were not very encouraging. The silicone seems to be able to 'move' within the block and gets eventually disrupted after/while touching the blade. I guess, because of the missing stable connection between the silicone and the embedding medium. Another problem is swelling of the polymer in the organic solvent-but, I can deal with that. Any help will be greatly appreciated. Thanks, Klaus Klaus Kruttwig INM-Leibniz Institute for New Materials Saarbruecken, Germanz -------------------------------------------------------------------------------- INM - Leibniz-Institut f?r Neue Materialien gGmbH Campus D2 2, 66123 Saarbr?cken Homepage: www.leibniz-inm.de Sitz der Gesellschaft: Saarbr?cken Rechtsform: gGmbH Amtsgericht Saarbr?cken, HRB 8525 Gesch?ftsf?hrer: Prof. Dr. Eduard Arzt (Vorsitz), Prof. Dr. Ar?nzazu del Campo, G?nter Weber Kuratoriumsvorsitzende: Dr. Susanne Reichrath USt.-ID: DE 138167776 -------------------------------------------------------------------------------- From CDavis at che-east.org Wed Dec 14 12:32:17 2016 From: CDavis at che-east.org (Cassie P. Davis) Date: Wed, 14 Dec 2016 18:32:17 +0000 Subject: [Histonet] CLIA classifications Message-ID: <5C815EADE724D14AA0CC8F037C4185F079B36F3F@SB01MSTMBX13.sb.trinity-health.org> Hi Histofolks, We are having a friendly discussion as to the CLIA classification of job titles & I could use some help clarifying: Histology microtomy & H/E- I'm say it would be moderate complexity others are arguing otherwise... Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Timothy.Morken at ucsf.edu Wed Dec 14 13:41:06 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 14 Dec 2016 19:41:06 +0000 Subject: [Histonet] CLIA classifications In-Reply-To: <5C815EADE724D14AA0CC8F037C4185F079B36F3F@SB01MSTMBX13.sb.trinity-health.org> References: <5C815EADE724D14AA0CC8F037C4185F079B36F3F@SB01MSTMBX13.sb.trinity-health.org> Message-ID: <761E2B5697F795489C8710BCC72141FF88E3212C@ex07.net.ucsf.edu> Cassandra, for CLIA, CAP and Joint Commission, microtomy and H&E staining (as well as embedding and special stains) are classified as "specimen processing" and do not come under the complexity designation. The reason is that the histotechs are not doing any "testing" which is defined as interpreting a result for diagnosis and signing off on a diagnostic report. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cassie P. Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, December 14, 2016 10:32 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CLIA classifications Hi Histofolks, We are having a friendly discussion as to the CLIA classification of job titles & I could use some help clarifying: Histology microtomy & H/E- I'm say it would be moderate complexity others are arguing otherwise... Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper at chla.usc.edu Wed Dec 14 18:57:42 2016 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Thu, 15 Dec 2016 00:57:42 +0000 Subject: [Histonet] BRAF V600E In-Reply-To: References: Message-ID: Thank you to all who replied to my inquiry about BRAF on the Bond Max. As always, I'm truly grateful for the valuable resource that is Histonet. Happy Holidays everyone! Brian -----Original Message----- From: Cooper, Brian via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, December 13, 2016 2:50 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] BRAF V600E Good Afternoon Histonet! It's been a few years since I posted this question initially; thought I'd give it a try again. Is anyone successfully utilizing Roche's BRAF V600e (VE1) (or Spring Bioscience's) antibody on a Leica Bond Max? If you are, I'd really appreciate hearing how you did it. This antibody is quite expensive, and we don't particularly want to burn through massive amounts of money trying to reinvent the wheel. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From bchick2g at hotmail.com Wed Dec 14 19:19:18 2016 From: bchick2g at hotmail.com (Amanda Miele) Date: Thu, 15 Dec 2016 01:19:18 +0000 Subject: [Histonet] High/Low thermometed Message-ID: Hello, I am wondering what thermometer you all may have found that is really easy to use with little to no complications to record high/low temperatures and humidity rates in the lab. Thank you! Amanda Miele, HT (ASCP) From CDavis at che-east.org Thu Dec 15 07:07:42 2016 From: CDavis at che-east.org (Cassie P. Davis) Date: Thu, 15 Dec 2016 13:07:42 +0000 Subject: [Histonet] THANK YOU for responses CLIA and Histology Message-ID: <5C815EADE724D14AA0CC8F037C4185F079B36FB3@SB01MSTMBX13.sb.trinity-health.org> Thank you to all who messaged me about this! Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Timothy.Morken at ucsf.edu Thu Dec 15 10:04:31 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 15 Dec 2016 16:04:31 +0000 Subject: [Histonet] High/Low thermometed In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF88E323B6@ex07.net.ucsf.edu> Amanda, we use the Fisher Scientific traceable (NISH certificated) for general hi/lo recording. It just records Hi and Lo over whatever time period until it is reset. It does not tell you when the hi/lo occurred. You can get a model that has a 30 day memory and records the time of the hi and lo each day and overall for the period. They have many models so just look for one that fits your needs. One caution - when using be sure it is displaying the actual read out. We found a lab who had the display set to the Hi/Lo setting screen -so always shows the hi and lo parameters, not the actual temperature. They had been recording those same "temperatures" for weeks! Tim -----Original Message----- From: Amanda Miele via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, December 14, 2016 5:19 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] High/Low thermometed Hello, I am wondering what thermometer you all may have found that is really easy to use with little to no complications to record high/low temperatures and humidity rates in the lab. Thank you! Amanda Miele, HT (ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lmarie08 at uga.edu Thu Dec 15 10:23:12 2016 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Thu, 15 Dec 2016 16:23:12 +0000 Subject: [Histonet] holidays SOP Message-ID: Hello all in Histo Land, I was wondering if anyone out there works in a Histo Lab that shuts down for the holidays? We are closed the week of Christmas and I am preparing the laboratory for sitting unattended for a week. If anyone else is in the same boat, do you have any tips for what you do to shut down the lab? We are planning on emptying the strainers and keeping the coverslipper nozzle submerged in xylene as air-tight as possible. Not sure what or if we should bother the processors... Thanks and Happy Holidays! From jvickroy at SpringfieldClinic.com Thu Dec 15 12:32:10 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Thu, 15 Dec 2016 18:32:10 +0000 Subject: [Histonet] Logging expiration dates and receiving of chemicals Message-ID: <9B1A1501A800064397369BD8072E6BCA06602015@E2K10DB.springfieldclinic.com> One of my techs has asked about a practice that we have been doing for some time. Occasionally we find that we are doing something just because it has been done in the past and as we all know sometimes we get bogged down with things that are just not necessary. When we order chemicals such as alcohols, clarifier, bluing, hematoxylin, etc. each chemical with their lot number and expiration date is listed in a log book. Of course we also date the container when we receive each chemical and we also make sure that all of the chemicals are used within expiration dates. The question is whether it is really necessary to keep a log of information besides what is written on the container. In addition we are a new lab and have started to do some special stains inhouse in the lab. (PAS, PAS for fungus, AFB) We started with purchasing kits and have now simply ordered the chemicals in bulk. Do we need to test each lot of chemical that we use for the stains? We run a control with every run and I don't feel it is necessary to test the chemicals additionally. One of the companies we ordered the stain kit from sent us control test slides and my tech wondered if we should run one of the control test slides before we used the kit. I know that in IHC staining there are rules for using, testing, comparing with previous lots,etc. for kits however since we are going to use bulk chemicals and we run a control each time I don't want to do extra unnecessary work. It also seems to me that over the years the CAP questions governing routine special staining has been reduced more and more. At this point we don't run any IHC stains and therefore I am not worried about detections kits, new lots of antibodies, etc. Just need to know what most do with special stain chemicals. I would appreciate your thoughts before we continue our practice or scale down some of the documentation. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From Blanca.Lopez at UTSouthwestern.edu Thu Dec 15 13:09:04 2016 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Thu, 15 Dec 2016 19:09:04 +0000 Subject: [Histonet] storage big samples Message-ID: Hello! I was wondering if anybody has an idea how can to storage the whole prostate tissue in -80c. What kind of container we can buy or media or bags, or anything to keep it safe.. Also, I need a good advice in how to cut frozen and re-storage in -80 C. How can I removed the oct without expose the tissue too much. The first time I thaw the tissue in water to remove the oct because is to big that is really hard to place it on my mold. I was able to cut frozen slides but the procedure that they are doing did not work. Do I am, doing something wrong? I really need a good advice in handling cutting frozen, stain them and storage them back in -80c. Any suggestions that can help me? thanks Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From Blanca.Lopez at UTSouthwestern.edu Thu Dec 15 15:47:29 2016 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Thu, 15 Dec 2016 21:47:29 +0000 Subject: [Histonet] processing question Message-ID: If I have tissues being held in PBS after fixation. It is ok to load them in the processor starting with formalin or Do I need to skip this step and started in alcohol? Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: Blanca.Lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From LRaff at uropartners.com Fri Dec 16 07:53:17 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 16 Dec 2016 13:53:17 +0000 Subject: [Histonet] Lab inspired blog post for Friday Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF1114936E@COLOEXCH01.uropartners.local> Have a good weekend. If you are in part of this deep chill, stay warm. http://www.chicagonow.com/downsize-maybe/2016/12/cant-make-up-your-mind-it-will-be-better-in-the-morning-a-look-at-decision-fatigue/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From PKRichar at gundersenhealth.org Fri Dec 16 09:23:35 2016 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Fri, 16 Dec 2016 15:23:35 +0000 Subject: [Histonet] Grossing small biopsies Message-ID: <998284C32F61104CA0BEFFFFCF6F90FD6D1FD97C@LXEXMB01.gundluth.org> I am wondering how many techs gross small biopsies? If you do is this part of your role as a histology tech or do you have a different job description? Cordially, Pam ~ +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org From LRaff at uropartners.com Fri Dec 16 09:56:31 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 16 Dec 2016 15:56:31 +0000 Subject: [Histonet] Grossing small biopsies In-Reply-To: <998284C32F61104CA0BEFFFFCF6F90FD6D1FD97C@LXEXMB01.gundluth.org> References: <998284C32F61104CA0BEFFFFCF6F90FD6D1FD97C@LXEXMB01.gundluth.org> Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF1114C672@COLOEXCH01.uropartners.local> All our specimens are biopsies. Our minimum requirement for grossing biopsies is a BA in a science field and then our on-site training. We have two dedicated lab assistants trained in this manner. We use one of our histotechs who was trained in grossing elsewhere as a 3rd option, but it is not in the general histotech job description. -------------------------- http://www.chicagonow.com/downsize-maybe/2016/12/cant-make-up-your-mind-it-will-be-better-in-the-morning-a-look-at-decision-fatigue/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: Richardson, Pam K via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, December 16, 2016 9:24 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Grossing small biopsies I am wondering how many techs gross small biopsies? If you do is this part of your role as a histology tech or do you have a different job description? Cordially, Pam ~ +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun at hhchealth.org Fri Dec 16 11:55:51 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 16 Dec 2016 17:55:51 +0000 Subject: [Histonet] Aluminum Testing Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E953D8D83@HHCEXCHMB03.hhcsystem.org> Has anyone ever heard of testing a tissue or body fluid sample for "accrued aluminum"? We have a child who has had multiple immunizations and the parents wanted the specimen tested. Unfortunately, none of this was communicated to us prior to the procedure being performed. Thank you and "Happy Holidays" to everyone. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From mcginnessjamie at yahoo.com Sat Dec 17 09:20:01 2016 From: mcginnessjamie at yahoo.com (Jamie McGinness) Date: Sat, 17 Dec 2016 15:20:01 +0000 (UTC) Subject: [Histonet] Kawamoto's film method References: <1302624563.7012588.1481988001973.ref@mail.yahoo.com> Message-ID: <1302624563.7012588.1481988001973@mail.yahoo.com> I am looking into trying the Kawamoto's film method. ?I have the order sheet for the cryofilm sheets and other supplies. ?I did have a few questions. 1. ?I was wondering if anyone knows when you order a sheet how many slides can be produced from that sheet? ?2. ?Does anyone know what the transfer film is??3. ?What are the advantages to using their SCCM mounting medium ?and the difference between the four types offered G1, R1, R2, R3?4. ?What are the advantages of using heir SCEM embedding medium and the difference between the SCEM and SCEM L1? ?5. ?Does anyone know the differences of the cryofilm 2C(9), 2C(10), and 3C(16UF)? Thanks so much for any information you may have. Jamie?? From carl.hobbs at kcl.ac.uk Sat Dec 17 12:49:02 2016 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sat, 17 Dec 2016 18:49:02 +0000 Subject: [Histonet] Immunohelp website? Message-ID: Hi I am grateful for the opportunity to load IHC/IF etc images on this website. Thank you for creating it. Shame that more Immunopeople do not upload their images. Why do they not do this? Perhaps Histonet would be interested in expanding? Thus , any chance of you Main guys creating an Immunoportal -equivilent?? A great website, devised /run by the excellent Hogne! Hogne is long gone, sadly ( sure, only re his website: I am sure he is alive and well....I wish him well) Hogne had a Q and A , a methods and an image module. Histonet jest has a general Q forum? Which is invaluable but....too broad? To be sure...I could be missing the point Respectfully, carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From turkekul at gmail.com Sat Dec 17 13:05:54 2016 From: turkekul at gmail.com (Mesru T) Date: Sat, 17 Dec 2016 14:05:54 -0500 Subject: [Histonet] Histonet Digest, Vol 157, Issue 13 In-Reply-To: References: Message-ID: Hi Jamie, 1. The sheets have adhesive part and holding non-adhesive part. The sheet is as big as a regular A4 size. The length of the individual tapes is fixed but you can cut the sheets in different widths depending on the width of the sample. You can also order sheets with different fixed lengths. Probably 20-30 sectioning tapes can be generated from one sheet. 2. Nobody knows the chemistry of the transfer film except the creator. 3. You can use any media if you dehydrate you can use permanent or if not aqueous media. Their media is better optimized for the tape optics for imaging. 4. Regular OCT does not produce as good sections. SCEM works great. SCEM L1 is new and improved product. I have not tried. 5. The 2C cryofilms are the old versions. The new and improved version is 3C(16UF). The improved version produces better sections. Mesruh Turkekul > I am looking into trying the Kawamoto's film method. ?I have the order > sheet for the cryofilm sheets and other supplies. ?I did have a few > questions. > 1. ?I was wondering if anyone knows when you order a sheet how many slides > can be produced from that sheet? ?2. ?Does anyone know what the transfer > film is??3. ?What are the advantages to using their SCCM mounting medium > ?and the difference between the four types offered G1, R1, R2, R3?4. ?What > are the advantages of using heir SCEM embedding medium and the difference > between the SCEM and SCEM L1? ?5. ?Does anyone know the differences of the > cryofilm 2C(9), 2C(10), and 3C(16UF)? > Thanks so much for any information you may have. > Jamie?? > > > From abtdhu at gmail.com Sun Dec 18 11:00:01 2016 From: abtdhu at gmail.com (Dorothy Hu) Date: Sun, 18 Dec 2016 12:00:01 -0500 Subject: [Histonet] Histonet Digest, Vol 157, Issue 13 Kawamoto's film method (Jamie McGinness) In-Reply-To: References: Message-ID: <276CFD56-4B47-467C-B0D5-24EF7C22B74B@gmail.com> Please check this web for the answer of your question. http://section-lab.jp/English/Referece%20E.htm Dorothy Hu > > > I am looking into trying the Kawamoto's film method. ?I have the order sheet for the cryofilm sheets and other supplies. ?I did have a few questions. > 1. ?I was wondering if anyone knows when you order a sheet how many slides can be produced from that sheet? ?2. ?Does anyone know what the transfer film is??3. ?What are the advantages to using their SCCM mounting medium ?and the difference between the four types offered G1, R1, R2, R3?4. ?What are the advantages of using heir SCEM embedding medium and the difference between the SCEM and SCEM L1? ?5. ?Does anyone know the differences of the cryofilm 2C(9), 2C(10), and 3C(16UF)? > Thanks so much for any information you . From jriggleman at globusmedical.com Mon Dec 19 09:23:29 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Mon, 19 Dec 2016 15:23:29 +0000 Subject: [Histonet] High/Low thermometed In-Reply-To: References: Message-ID: Hi Amanda, We use T&D, they record temperature and humidity. http://www.tandd.com/product/tr7wfnw_series.html -Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. -----Original Message----- From: Amanda Miele [mailto:bchick2g at hotmail.com] Sent: Wednesday, December 14, 2016 8:19 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] High/Low thermometed Hello, I am wondering what thermometer you all may have found that is really easy to use with little to no complications to record high/low temperatures and humidity rates in the lab. Thank you! Amanda Miele, HT (ASCP) From gayle.callis at bresnan.net Mon Dec 19 11:13:16 2016 From: gayle.callis at bresnan.net (Gayle Callis) Date: Mon, 19 Dec 2016 10:13:16 -0700 Subject: [Histonet] Kawamoto protocol online from Rowe lab Message-ID: <001f01d25a1b$30c9bcf0$925d36d0$@bresnan.net> Go to this link: http://bonebase.org/bonebase/ Then click on Skeletal Phenotyping, then go to Histomorphometry to see how to use Kawamoto tape system with all the sample handling. Photos and an excellent video titled High-Throughput, mutliimage cryohistology of mineralized tissues is invaluable, with Protocol and Materials. You can also access a pdf of protocol. Video access is on home page of this website. You will see cryomicrotomy, snap freezing and other techniques to help you. Good Luck Gayle Callis HTL/HT/MT(ASCP) From cforster at umn.edu Mon Dec 19 11:18:19 2016 From: cforster at umn.edu (Colleen Forster) Date: Mon, 19 Dec 2016 11:18:19 -0600 Subject: [Histonet] Kawamoto protocol online from Rowe lab In-Reply-To: <001f01d25a1b$30c9bcf0$925d36d0$@bresnan.net> References: <001f01d25a1b$30c9bcf0$925d36d0$@bresnan.net> Message-ID: Thanks Gayle....this is the system the pathologist wanted to but....$3000.00 and he had no money.... I want to try and work with more reasonably priced ideas first.....not sure I will ever get this one down....I think Ill have better luck with bugs! I will watch this video and read the notes.... C On Mon, Dec 19, 2016 at 11:13 AM, Gayle Callis via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Go to this link: http://bonebase.org/bonebase/ > > > > Then click on Skeletal Phenotyping, then go to Histomorphometry to see how > to use Kawamoto tape system with all the sample handling. Photos and an > excellent video titled High-Throughput, mutliimage cryohistology of > mineralized tissues is invaluable, with Protocol and Materials. You can > also access a pdf of protocol. Video access is on home page of this > website. You will see cryomicrotomy, snap freezing and other techniques > to > help you. > > > > Good Luck > > > > Gayle Callis > > HTL/HT/MT(ASCP) > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From wbenton at cua.md Mon Dec 19 12:38:10 2016 From: wbenton at cua.md (Walter Benton) Date: Mon, 19 Dec 2016 18:38:10 +0000 Subject: [Histonet] Temp Assignment for Chesapeake Urology Message-ID: <1e399748a94440f88cf4aaa28fe192f3@MAIL01.GCU-MD.local> To all: Our lab is in search of temp help to cover an extended absence by a staff member. The ideal candidate will have Prostate Grossing experience and be eligible to gross based on CLIA 88 Guidelines. In addition, this person should be a strong prostate biopsy cutter and embedder. The assignment will be 4 weeks in length. This will be a 40hr/week assignment. The shift may start as early as 4 am or 6am depending on the individuals skills and abilities to meet the needs of the lab. If interested, please send your resume along with desired salary to: mhwang at cua.md and myself wbenton at cua.md From: Walter Benton Sent: Monday, December 19, 2016 12:07 PM To: histonet at lists.utsouthwestern.edu Cc: Michael Hwang Subject: Temp Assignment for Chesapeake Urology To all: Our lab is in search of temp help to cover an extended absence by a staff member. The ideal candidate will have Prostate Grossing experience and be eligible to gross based on CLIA 88 Guidelines. In addition, this person should be a strong prostate biopsy cutter and embedder. The assignment will be 4 weeks in length. This will be a 40hr/week assignment. The shift may start as early as 4 am or 6am depending on the individuals skills and abilities to meet the needs of the lab. If interested, please send your resume along with desired salary to: mhwang at cua.md and myself wbenton at cua.md Thanks Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From garethdavisyuma at gmail.com Mon Dec 19 13:07:53 2016 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Mon, 19 Dec 2016 12:07:53 -0700 Subject: [Histonet] Processing time for small biopsies Message-ID: Hi, Recently I saw a post from someone looking for a protocol for processing small biopsies. I looked on the histonet search, but found that to be too time consuming to locate the post. Anyway, I hoping to get some opinions on good process times for small biopsies. I recently changed my paraffin brand and now I have chatter on my sections. -- Ms. Gareth B. Davis, HT, QIHC (ASCPcm) Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From jamie.erickson at abbvie.com Mon Dec 19 13:15:44 2016 From: jamie.erickson at abbvie.com (Erickson, Jamie E) Date: Mon, 19 Dec 2016 19:15:44 +0000 Subject: [Histonet] Kawamoto protoco Message-ID: <4af12bb194a2472f97df7d42e89d5cdd@USAASECSM084.R0018.COLLABORATION.ECS.HP.COM> Hello histo-neters, I was able to purchase this system many years back from Dr. Kawamoto because we had many mouse bones we wanted to do IHC, ISH etc. on. After watching the video it was clear that this was not going to be fast and easy. Our impressions were that this was going to take a lot of effort, time and the investigators did not move forward with testing out the kit. We use the Kawamoto tape in some situations as it can give good sections but we did not make this a standard SOP. If I remember a few issues we had were that the staining was done completely on the sectioned tape (floating on flowing water) then the tape was mounted to a glass slide under a coverslip. As with everything the devil is in the details and there were a lot of details, from embedding samples with the mounting media exactly as stated in protocol to sectioning with D-profile blades or disposable but these had to be special (thicker) blades with a special blade holder angled if I'm remembering right. We modified things and made a method that worked good enough but nothing like the slides he sends with the kit , they are perfect. He is truly a master of this craft. His protocol may have changed from ~ 2009 but we thought it was going to take a lot of training and time which is in short supply in industry. I still like fix/ decal (formic acid or EDTA) and sucrose protect I think this give me good results and great sections when sectioned with tape transfer.. These are my thoughts I hope it helps, Jamie ericKson, MS Scientist II AbbVie Bioresearch Center, Inc. 100 Research Drive Worcester, MA 01605 OFFICE +1 508-688-3134 EMAIL jamie.erickson at abbvie.com This communication may contain information that is proprietary, confidential, or exempt from disclosure. If you are not the intended recipient, please note that any other dissemination, distribution, use or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately by telephone or by return e-mail and delete it from his or her computer. From joyce.weems at emoryhealthcare.org Mon Dec 19 13:19:07 2016 From: joyce.weems at emoryhealthcare.org (Weems, Joyce K.) Date: Mon, 19 Dec 2016 19:19:07 +0000 Subject: [Histonet] Peloris problem Message-ID: Hello Everyone! Has anyone ever had a problem with a Leica Peloris, in which an incorrect reagent was pulled or missed entirely by the instrument - no user error? Thanks and Merry Christmas/Happy Holidays! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax 770-380-8099 Cell joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From allanvv at gmail.com Mon Dec 19 13:57:50 2016 From: allanvv at gmail.com (Allan Wang) Date: Mon, 19 Dec 2016 14:57:50 -0500 Subject: [Histonet] Adding glycerol to concentrated antibodies Message-ID: Hello all, I noticed CST sells most of their antibodies with 50% glycerol and recommends -20C storage and no aliquoting. They remain in liquid form in the freezer. A little bit more difficult to pipette precisely due to the viscosity and temp difference, but it seems like a good trade-off for easy handling and storage. Some other manufacturers also supply with 40% or 50% glycerol, but only for a few antibodies and it isn't common. Has anyone experienced problems with their antibodies from adding glycerol 1:1? I know they shouldn't be more than 1% or so in the final working solution, but given that most IHC antibodies are diluted 1:100 or greater, this doesn't seem to be an issue. I tested on a few antibodies I had and saw no difference in staining, but I haven't done long-term testing yet. I'll probably add a little bit more sodium azide too. Thanks, Allan From abtdhu at gmail.com Mon Dec 19 13:59:18 2016 From: abtdhu at gmail.com (Dorothy Hu) Date: Mon, 19 Dec 2016 14:59:18 -0500 Subject: [Histonet] Histonet Digest, Vol 157, Issue 15 Kawamoto methods Message-ID: Mounting media, G1 for regular application. R2 is suitable for hard tissue. The difference between the SCEM and SCEM L1 embedding medium is for different temperature? The cryofilm 2C(9) can be immersed in acetone and xylene, 2C(10) is for polarized microscope, and 3C(16UF) is good for many conventional staining, TRAP, ALP and IHC posibble ISH. Just order a kit and mounting media R2, you should be able to make section. Dorothy Hu From nto at stowers.org Tue Dec 20 07:10:19 2016 From: nto at stowers.org (Thomas, Nancy) Date: Tue, 20 Dec 2016 13:10:19 +0000 Subject: [Histonet] Decal following ISH? Message-ID: Hello all, I received whole mount samples in 70% ethanol for paraffin processing. The samples are snail embryos and the researcher already did in-situ on them. Because they are of varying ages, some of the shells will section without decal, but some will need it. However, this step was not done before the ISH staining. Does anyone know if decalcification can follow an ISH procedure? I have searched a few protocols online, and each of them did the decal before hybridization. I have not yet found one where the decal followed the procedure. My plan is to start with one embryo and try it to see what happens. Maybe the signal will remain, or maybe not. Has anyone done it this way and know the answer before I try? Thank you, Nancy Thomas Senior Lab Manager, Histology Core Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 From gayle.callis at bresnan.net Tue Dec 20 09:32:29 2016 From: gayle.callis at bresnan.net (Gayle Callis) Date: Tue, 20 Dec 2016 08:32:29 -0700 Subject: [Histonet] Decal following ISH? In-Reply-To: References: Message-ID: <003a01d25ad6$46ed8c80$d4c8a580$@bresnan.net> Dear Nancy, Try using EDTA after ISH. Small embryos with shells will probably take very little time in 14% Tetra Sodium EDTA in PBS (we use Dulbeccos PBS) adjust the pH down to 7.4 - 7.6 with glacial acetic acid although some use hydrochloric acid. We adjusted the pH using constant stirring on a magnetic stirrer with single electrode immersed in solution allowing continuous readout on the pH meter. Basically, you are titrating the pH down with acid using a plastic Pasteur pipette, very easy. Tetra Sodium EDTA is very alkaline, hence adjusting pH down with one of these acids is necessary. 7.4 is normal pH for Dulbeccos PBS and 7.6 is a working pH for TRIS buffered Saline (TBS) or a use a pH compatible with ISH protocol. EDTA will not be bothered by samples immersed in alcohol and you may be able to preserve the ISH work done. It is worth a try. In the future, I suggest the researcher do fixation, then EDTA decalcification before ISH particularly if the signal is ruined on snails with shells. I bet it would make his results better too - no shell to deter penetration of reagents. Good luck and let us know if you have success. Happy Holidays!!! Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: Thomas, Nancy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, December 20, 2016 6:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Decal following ISH? Hello all, I received whole mount samples in 70% ethanol for paraffin processing. The samples are snail embryos and the researcher already did in-situ on them. Because they are of varying ages, some of the shells will section without decal, but some will need it. However, this step was not done before the ISH staining. Does anyone know if decalcification can follow an ISH procedure? I have searched a few protocols online, and each of them did the decal before hybridization. I have not yet found one where the decal followed the procedure. My plan is to start with one embryo and try it to see what happens. Maybe the signal will remain, or maybe not. Has anyone done it this way and know the answer before I try? Thank you, Nancy Thomas Senior Lab Manager, Histology Core Stowers Institute for Medical Research 1000 E. 50th Street Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt at pa-ucl.com Tue Dec 20 12:18:30 2016 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Tue, 20 Dec 2016 18:18:30 +0000 Subject: [Histonet] ConfirmMDx Message-ID: Hi All- Looking for feedback on experience with prostate specimens going to ConfirmMDx for testing: Reimbursement, physician satisfaction, blocks and slides or whatever else you have experienced. Thank you, Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From wweber at terpmail.umd.edu Tue Dec 20 12:41:01 2016 From: wweber at terpmail.umd.edu (William Weber) Date: Tue, 20 Dec 2016 13:41:01 -0500 Subject: [Histonet] I am having trouble with testis tissue slide preps. Message-ID: I am having trouble with testis tissue slide preps. I am fixing and embedding rodent testis in paraffin, however the tissue is not embedding completely in the wax. It falls out of the block and when it stays in the block the microtome will chunk it off. My Protocol 1. fixation in Bouin?s fixative 2. dehydration to 100% ethanol 3. 2 1hr paraffin baths 4. paraffin embedding - I use a little was in the mold before placing the tissue in, then I place the cassette and fill the mold the paraffin this whole time is molten. I immediately move it to a cold plate to solidify 5. Sectioning -I am able to get good ribbons, but there are holes in the ribbon where the tissue should be. It either falls from the ribbon of is pushed back into the block, often when it does cut a section it cuts a section much thicker than set for (e.g. 100 microns instead of 5). If anyone has any suggestions it would be greatly appreciated. -- W.David Weber, M.Sc. Pre-Doctoral Candidate University of Maryland Alt EMAIL; widawe at hotmail.com TEL; (301)405-5737 From jvickroy at SpringfieldClinic.com Tue Dec 20 14:39:32 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Tue, 20 Dec 2016 20:39:32 +0000 Subject: [Histonet] Formaldehyde filters in Grosslab Senior Message-ID: <9B1A1501A800064397369BD8072E6BCA06605B6F@E2K10DB.springfieldclinic.com> It seems to me that we are replacing filters much too often on our Grosslab senior grossing station. I do realize that there are so many variables but would be interested in: By the way we only gross small biopsies, gyn, gi, derm) a. How often others replace Grosslab filters? b. How others have maybe lengthened the time that the filters last? (We do use formalin absorbing and neutralizing pads.) Any other experiences or help would be appreciated. Also we do monitor the formalin levels with badges and our levels every six months are way below safety levels. Badges are worn by the techs doing the gross. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From jriggleman at globusmedical.com Wed Dec 21 15:22:04 2016 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Wed, 21 Dec 2016 21:22:04 +0000 Subject: [Histonet] Coverslipping Message-ID: Hello, Would anyone mind sharing their procedure after coverslipping a slide? Do you let the slide sit and dry? Do you use xylenes to wipe of the extra mounting medium immediately? I noticed after storing the slides in a slide box, a lot of dust collects. Is this because of the xylenes? Should I wipe the slide with alcohol or wrap them to prevent this? What do you use to wrap your slides? Please keep in mind they are large, 2x3 slides. Thank you for all of your input, Best, Jessica Riggleman _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From ajayiifeoluwa at gmail.com Wed Dec 21 23:08:40 2016 From: ajayiifeoluwa at gmail.com (Ifeoluwa Ajayi) Date: Thu, 22 Dec 2016 06:08:40 +0100 Subject: [Histonet] Coverslipping In-Reply-To: References: Message-ID: After cover slipping we just allow the slide to stay on the bench for it to dry. We then carefully remove the extra mountant with the aid of scalpel blade. Of course dust may cover it but cotton wool and xylene this can be removed. Ajayi Ifeoluwa BMLS, MSc University College Hospital UCH Ibadan, Nigeria. On Dec 21, 2016 10:31 PM, "Jessica Riggleman via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > Hello, > > Would anyone mind sharing their procedure after coverslipping a slide? > > Do you let the slide sit and dry? Do you use xylenes to wipe of the extra > mounting medium immediately? > > I noticed after storing the slides in a slide box, a lot of dust collects. > Is this because of the xylenes? Should I wipe the slide with alcohol or > wrap them to prevent this? What do you use to wrap your slides? > > Please keep in mind they are large, 2x3 slides. > > Thank you for all of your input, > > Best, > Jessica Riggleman > > > _____________________________________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > > Confidentiality Note: This email is confidential and intended solely for > the use of the individual to whom it is addressed. If you are not the > intended recipient, be advised that you have received this email in error > and that any use, dissemination, forwarding, printing, or copying of this > email is strictly prohibited. If you have received this email in error > please contact the sender. Any views or opinions presented are solely those > of the author and do not necessarily represent those of Globus Medical, > Inc. Although this email and any attachments are believed to be free of any > virus or other defects which might affect any computer or IT system into > which they are received, no responsibility is accepted by Globus Medical, > Inc. for any loss or damage arising in any way from the receipt or use > thereof. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Karen.Heckford at DignityHealth.org Thu Dec 22 06:22:00 2016 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Thu, 22 Dec 2016 05:22:00 -0700 Subject: [Histonet] Tissue Tek prisma stainer Message-ID: Good Morning and Happy Holidays, I was just wondering people that have the Prisma, does the monitor on the top right corner swivel and can be positioned in different positions? Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From rcharles at pa.gov Thu Dec 22 06:59:07 2016 From: rcharles at pa.gov (Charles, Roger) Date: Thu, 22 Dec 2016 12:59:07 +0000 Subject: [Histonet] slider printer Message-ID: Hello, We are looking into barcoding in our histology lab. Does anyone have recommendations on which slide printer and cassette printer works best? Pros and cons? Thanks Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us From lmarie08 at uga.edu Thu Dec 22 07:35:23 2016 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Thu, 22 Dec 2016 13:35:23 +0000 Subject: [Histonet] Wright's stain Message-ID: Hello all in histoland- Does anyone out there have a recipe for preparing Wright's stain from powder? Our pathologist wants us to do Sheehan's modification Giemsa and we don't have any commercially prepared Wrights left but we do have an old bottle of Wright's powder. Happy Holidays! L From victor_tobias at comcast.net Thu Dec 22 10:11:53 2016 From: victor_tobias at comcast.net (victor_tobias at comcast.net) Date: Thu, 22 Dec 2016 08:11:53 -0800 Subject: [Histonet] slider printer In-Reply-To: References: Message-ID: Charles, If you have your heart set on a slide printer, go with the Sakura one. A Zebra printer with the right labels and ribbon is an excellent alternative. Cassette printing General Data hands down the best. Victor Sent from Mail for Windows 10 From: Charles, Roger via Histonet Sent: Thursday, December 22, 2016 5:00 AM To: Histonet (histonet at lists.utsouthwestern.edu) Subject: [Histonet] slider printer Hello, We are looking into barcoding in our histology lab. Does anyone have recommendations on which slide printer and cassette printer works best? Pros and cons? Thanks Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun at hhchealth.org Thu Dec 22 13:36:39 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Thu, 22 Dec 2016 19:36:39 +0000 Subject: [Histonet] IHC for ALDH1 Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E953DD6A3@HHCEXCHMB03.hhcsystem.org> Is anyone doing IHC testing for Aldehyde Dehydrogenase 1 (ALDH1)? Evidently, it is a prognostic maker used for breast CA. Happy Holidays everyone! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From j.benavides at eae.csic.es Fri Dec 23 11:12:12 2016 From: j.benavides at eae.csic.es (Julio Benavides) Date: Fri, 23 Dec 2016 18:12:12 +0100 Subject: [Histonet] Processing of frozen material In-Reply-To: References: Message-ID: <20161223181212.Horde.ZIWxQUb6ytXy6pTSHkS6kQ9@webmail.csic.es> Hi there, I have frozen (-20?C) samples from skeletal muscle (sheep samples) that now requiere processing and cutting for HE and IHC. Any advice on how to proceed? better to put them in formalin while still frozen or first thaw them? A good way to thaw them preserving the most of the histological architecture? Thanks a lot for your help and have a very nice Chritsmas. All the best for 2017! Julio From aj.taylor at blueyonder.co.uk Mon Dec 26 16:15:02 2016 From: aj.taylor at blueyonder.co.uk (taylor alan) Date: Mon, 26 Dec 2016 22:15:02 +0000 (GMT) Subject: [Histonet] Wright's stain In-Reply-To: References: Message-ID: <1215428218.593897.1482790502013.JavaMail.open-xchange@oxbe7.tb.ukmail.iss.as9143.net> Hi Lauren With reference to your enquiry for the preparation of Wrights stain from Wrights stain powder I have found the original method of preparing Wrights stain in one of my favourite 'historical' pathology text books. Practical Bacteriology, Bloodwork and Parasitology. Eighth Edition. Edited by Rear Admiral Professor E R Stitt. USN. 1927. Who was the Director of the Naval Medical School in Washington and the Phillipines. Many older technicians and medical staff who served time in the Navy may remember this great text. Stitt details Wrights original method of preparing this stain, over a paragraph, cutting tothe chase the resulting dried precipitate is scraped from the filter paper for storage. The method for preparing a working solution is to weigh out 300mg of stain powder and add to this in a flask 100ml of pure (acetone free) methyl alcohol to dissolve.(All Romanowsky stains require this same alcohol). Attached to this paragraph is an additional recommendation by Wright to make a stock solution by weighing out 100mg of stain powder and dissolving this in 60ml of methyl alcohol, as above. For use filter off 20ml and add to the filtrate 5ml of pure methyl alcohol to make a working solution. I would be very happy to copy the whole paragraph for you, detailing the lengthy preparation of this stain if you wish. If you have a very aged bottle of this stain powder there is a very good chance that you will obtain better results than from a stain prepared in more recent years. We have dry powder stains that are more than 50 years old that give precise and beautiful results, these are used sparingly and are very precious to us here in our laboratory. Hoping you have success with your staining and the methodology detailed above is helpful to you and your colleagues. Best Regards Alan Taylor BSc(Hons), FRMS. Microtechnical Services Exeter. UK. > > On 22 December 2016 at 13:35 Lauren Sweeney via Histonet > wrote: > > > Hello all in histoland- > > Does anyone out there have a recipe for preparing Wright's stain from > powder? Our pathologist wants us to do Sheehan's modification Giemsa and we > don't have any commercially prepared Wrights left but we do have an old bottle > of Wright's powder. > > Happy Holidays! > > L > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From aj.taylor at blueyonder.co.uk Mon Dec 26 16:21:23 2016 From: aj.taylor at blueyonder.co.uk (taylor alan) Date: Mon, 26 Dec 2016 22:21:23 +0000 (GMT) Subject: [Histonet] Wright's stain In-Reply-To: <1215428218.593897.1482790502013.JavaMail.open-xchange@oxbe7.tb.ukmail.iss.as9143.net> References: <1215428218.593897.1482790502013.JavaMail.open-xchange@oxbe7.tb.ukmail.iss.as9143.net> Message-ID: <117206107.593945.1482790883314.JavaMail.open-xchange@oxbe7.tb.ukmail.iss.as9143.net> > On 26 December 2016 at 22:15 taylor alan wrote: > > > Hi Lauren > > With reference to your enquiry for the preparation of Wrights stain from > Wrights stain powder > > I have found the original method of preparing Wrights stain in one of my > favourite 'historical' pathology text books. Practical Bacteriology, Bloodwork > and Parasitology. Eighth Edition. Edited by Rear Admiral Professor E R Stitt. > USN. 1927. Who was the Director of the Naval Medical School in Washington and > the Phillipines. Many older technicians and medical staff who served time in > the Navy may remember this great text. > > Stitt details Wrights original method of preparing this stain, over a > paragraph, cutting tothe chase the resulting dried precipitate is scraped from > the filter paper for storage. The method for preparing a working solution is > to weigh out 300mg of stain powder and add to this in a flask 100ml of pure > (acetone free) methyl alcohol to dissolve.(All Romanowsky stains require this > same alcohol). > > Attached to this paragraph is an additional recommendation by Wright to > make a stock solution by weighing out 100mg of stain powder and dissolving > this in 60ml of methyl alcohol, as above. For use filter off 20ml and add to > the filtrate 5ml of pure methyl alcohol to make a working solution. > > I would be very happy to copy the whole paragraph for you, detailing the > lengthy preparation of this stain if you wish. If you have a very aged bottle > of this stain powder there is a very good chance that you will obtain better > results than from a stain prepared in more recent years. We have dry powder > stains that are more than 50 years old that give precise and beautiful > results, these are used sparingly and are very precious to us here in our > laboratory. > > Hoping you have success with your staining and the methodology detailed > above is helpful to you and your colleagues. > > Best Regards > > Alan Taylor BSc(Hons), FRMS. > > Microtechnical Services > > Exeter. UK. > > > > > > > On 22 December 2016 at 13:35 Lauren Sweeney via Histonet > > wrote: > > > > > > Hello all in histoland- > > > > Does anyone out there have a recipe for preparing Wright's stain > > from powder? Our pathologist wants us to do Sheehan's modification Giemsa > > and we don't have any commercially prepared Wrights left but we do have an > > old bottle of Wright's powder. > > > > Happy Holidays! > > > > L > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > From criley at dpspa.com Wed Dec 28 07:18:43 2016 From: criley at dpspa.com (Charles Riley) Date: Wed, 28 Dec 2016 08:18:43 -0500 Subject: [Histonet] Staining issues Message-ID: Our pathologists are complaining that the center of tissues are not staining properly in both our H&E's and IHC's. Can anyone provide some thoughts as to where this problem could be occurring? As a separate situation we are experiencing the tissues folding or falling off during the staining process. Both this issue and the staining issue are recent occurrences and we have not changed our process in any way from previous years. Does anyone think these problems are related? Why or why not? I have racked my brain with all the trouble shooting techniques I can think of so any help will greatly be appreciated -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From rjbuesa at yahoo.com Wed Dec 28 08:09:42 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 28 Dec 2016 14:09:42 +0000 (UTC) Subject: [Histonet] Staining issues In-Reply-To: References: Message-ID: <1098350279.3026417.1482934182938@mail.yahoo.com> Improper staining at the center and falling sections are typical consequences of poor fixation/infiltration.If you have changed nothing proceduraly, what about somebody "new" grossing and preparing thicker tissue slices?Ren? On Wednesday, December 28, 2016 8:34 AM, Charles Riley via Histonet wrote: Our pathologists are complaining that the center of tissues are not staining properly in both our H&E's and IHC's. Can anyone provide some thoughts as to where this problem could be occurring? As a separate situation we are experiencing the tissues folding or falling off during the staining process. Both this issue and the staining issue are recent occurrences and we have not changed our process in any way from previous years. Does anyone think these problems are related? Why or why not?? I have racked my brain with all the trouble shooting techniques I can think of so any help will greatly be appreciated -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed Dec 28 09:00:31 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 28 Dec 2016 10:00:31 -0500 Subject: [Histonet] RELIA Holiday Histology Job Alert!! Would you like to live closer to family and friends? Message-ID: <009201d2611b$22539540$66fabfc0$@earthlink.net> Hi Histonetters!! How are you today? I hope you are enjoying a wonderful Christmas week. I was thinking that I should drop you a quick line to let you know where some of my current opportunities are. Who Knows Maybe Something I Am Working On Today Might Get You Closer To The Family And Friends You Are Enjoying Time With OR Missing During The Holidays. If you or anyone you know is interested in more details I can be reached toll free at 866-607-3542 or relia1 at earthlink.net. If your dream location isn't listed below shoot me an email and let me know where you want to go! We currently have openings in these areas for histology managers, lead techs histotechs and mohs techs: California Washington State Texas Tennessee Wisconsin Missouri Wyoming Colorado Remember all of these positions are permanent and full time and have excellent benefits. Also in most cases the clients are assisting with relocation. And some are offering sign on bonuses!! All of them are eager to interview and hire today!! But are also willing to wait until after the holidays if you prefer. Thanks and have a great day!! Pam Right Place, Right Time, Right Move with RELIA! Happy Holidays! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From criley at dpspa.com Wed Dec 28 10:55:26 2016 From: criley at dpspa.com (Charles Riley) Date: Wed, 28 Dec 2016 11:55:26 -0500 Subject: [Histonet] CAP checklist help Message-ID: I need help with figuring out how to meet checklist # ANP.23420. We use the Leica Bond III and MAX to do our ISH slides. How do I test the temperature and how would you recommend recording the results? -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From edmartin26 at gmail.com Wed Dec 28 12:33:54 2016 From: edmartin26 at gmail.com (Eddie Martin) Date: Wed, 28 Dec 2016 13:33:54 -0500 Subject: [Histonet] CAP checklist help Message-ID: Hi Charles, The Leica BOND system displays the temperatures of the individual heating pads during the retrieval stage on your computer monitor. You can easily take a screenshot of your computer monitor and save it to a flash drive as a digital record. *A heating error would appear if the individual heating pad didn't reach 100 degrees. The Leica service engineer for the Bond can also provide documentation every time they service your IHC analyzer. You can also check your slide staining history and find cases that didn't stain well and individually check if the result was due to the temperature not reaching 100 degrees on that individual heating pad. Hope this helps. V/r, Eddie Martin, HTL, QIHC IHC Histotechnologist III Walter Reed National Military Medical Center > On Dec 28, 2016, at 11:55 AM, Charles Riley wrote: > > I need help with figuring out how to meet checklist # ANP.23420. We use > the Leica Bond III and MAX to do our ISH slides. How do I test the > temperature and how would you recommend recording the results? > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > From wbenton at cua.md Wed Dec 28 12:58:56 2016 From: wbenton at cua.md (Walter Benton) Date: Wed, 28 Dec 2016 18:58:56 +0000 Subject: [Histonet] CAP checklist help In-Reply-To: References: Message-ID: Leica sells a temperature verification kit that should meet your needs for CAP compliance and documentation. We use it for our Leica Thermobrites used in the processing of FISH. -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, December 28, 2016 11:55 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CAP checklist help I need help with figuring out how to meet checklist # ANP.23420. We use the Leica Bond III and MAX to do our ISH slides. How do I test the temperature and how would you recommend recording the results? -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From SteveM at mcclainlab.com Wed Dec 28 14:41:08 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Wed, 28 Dec 2016 20:41:08 +0000 Subject: [Histonet] Histonet Digest, Vol 157, Issue 21 the center In-Reply-To: References: Message-ID: <266A230E-979F-4143-9A78-A638478BA909@mcclainlab.com> What paraffin are you using? I heard a rumor one manufacturer changed formulations recently. Same brand name different formula. Center problems often manifest where insufficient fixation is a primary event or where rapid processing meets the limits of solution maintainance and tissue size. Rapid processing w inadequate alcohols is a silent offender. Sometimes you just replace every solution, just so you know where you a new starting point. If you suspect a processing problem as a cause, one can often achieve a better result by melting and re-embedding in new paraffin. By this I mean Allow to sit in the embedding center melted in a new mold, in fresh new paraffin for 60minutes. Then Change paraffin again and embed and cool. Not sure why it works, but cooking for a few more minutes does.improve sectioning in marginal tissues. Pathologists can also be the cause- Tissues which are crushed have aberrant staining patterns. XS handling prior to fixation can mess up staining. Raw tissues rushed through and on the processor. I am guilty of all of them in the past. Steve A. McClain, MD > On Dec 28, 2016, at 13:30, "histonet-request at lists.utsouthwestern.edu" wrote: > > Our pathologists are complaining that the center of tissues are not > staining properly in both our H&E's and IHC's. Can anyone provide some > thoughts as to where this problem could be occurring? > > > As a separate situation we are experiencing the tissues folding or falling > off during the staining process. Both this issue and the staining issue are > recent occurrences and we have not changed our process in any way from > previous years. From mwich at 7thwavelabs.com Wed Dec 28 16:44:16 2016 From: mwich at 7thwavelabs.com (Michele Wich) Date: Wed, 28 Dec 2016 22:44:16 +0000 Subject: [Histonet] FJC controls Message-ID: <4ADAF16E8518764ABF9DCEB73129AA752350345D@WAVE-EMAIL.7thwave.local> Does anyone have any good Fluoro Jade C controls that they would be willing to sell or possibly trade for some other control blocks? Thanks, Michele From tony.henwood at health.nsw.gov.au Wed Dec 28 16:56:49 2016 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 28 Dec 2016 22:56:49 +0000 Subject: [Histonet] Staining issues In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF014D8334@SVDCMBX-MEX008.nswhealth.net> Hi Charles, Many issues could be in play. Since the centre of the tissue is causing problems, then I would look at fixation; are the spaces around cells in the centre of the block wider than at the edges (conversely do the cells look like they are smaller than their counterparts at the edge)? If part of this tissue is lightly fixed, they seem to be more prone to heating effects. If the haematoxylin is paler (washed-out?) in the centre then it is possible that the slides have been heated above 70oC before H&E staining. This will also affect the IPX's. Maybe the oven is over-heating. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 29 December 2016 12:19 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Staining issues Our pathologists are complaining that the center of tissues are not staining properly in both our H&E's and IHC's. Can anyone provide some thoughts as to where this problem could be occurring? As a separate situation we are experiencing the tissues folding or falling off during the staining process. Both this issue and the staining issue are recent occurrences and we have not changed our process in any way from previous years. Does anyone think these problems are related? Why or why not? I have racked my brain with all the trouble shooting techniques I can think of so any help will greatly be appreciated -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From blayjorge at gmail.com Thu Dec 29 06:01:57 2016 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Thu, 29 Dec 2016 07:01:57 -0500 Subject: [Histonet] small *and* cheap circular microscope cover slips Message-ID: small *and* cheap circular microscope cover slips Dear Colleagues: I am looking for a reliable supplier of small (say, 12 mm or less) *and* cheap circular microscope cover slips. Note: I have already contacted suppliers available on the web but wish to have a larger choice. If you have recommendations, please email me directly, blayjorge at gmail.com Apologies for potential duplicate emails. Happy 2017, in gratefulness, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From taylor at prometheushealthcare.com Fri Dec 30 08:13:09 2016 From: taylor at prometheushealthcare.com (Taylor Rinaldi) Date: Fri, 30 Dec 2016 09:13:09 -0500 Subject: [Histonet] Happy New Year from Prometheus healthcare!! Message-ID: <08c101d262a6$d99a31a0$8cce94e0$@prometheushealthcare.com> Hi Histonetters! My name is Taylor Rinaldi, I'm the Recruiting Manager with Prometheus Healthcare. My team and I wanted to extend our warmest wishes to and your families as we enter 2017! If you are looking for a new Histology position in the new year, let Prometheus Healthcare be the first to know and we will inform you on all the opportunities available in your area! Need assistance staffing your the lab in 2017? We are top in our field at assisting hospitals and laboratories nationwide. We are able to fill all laboratory positions and have a team who specialize in histology bench and supervisory roles specifically. We have a database full of qualified, hardworking, and licensed Histotechs. All of whom are looking for the freshest and newest opportunities. Please don't hesitate to reach out if there is anything we may assist you with in this upcoming new year. Let's make the workplace in 2017 great together! Thank you all in advance, and HAPPY NEW YEAR! Taylor Rinaldi Recruiting Manager Prometheus Healthcare Office 866-857-1434 Taylor at prometheushealthcare.com From twheelock at mclean.harvard.edu Fri Dec 30 13:32:49 2016 From: twheelock at mclean.harvard.edu (Wheelock, Timothy R.) Date: Fri, 30 Dec 2016 19:32:49 +0000 Subject: [Histonet] Harvard Brain Bank Job Opportunity Message-ID: <69718C0B0B3C414D9F8E7214AD400CC99FCD4D9B@PHSX10MB11.partners.org> Hi Everyone: Well, at the age of 67 and after more than 30 years of service, I am retiring from the Harvard Brain Tissue Resource Center (the Brain Bank) at McLean Hospital in Belmont, Massachusetts. My last day is today, December 30th 2016. We are looking for someone to take over my position. If you are interested, please click on the following website. http://www.mcleanhospital.org/apply-work-mclean This website brings up the different positions currently open at McLean Hospital. The position is "Histologist Lab Manager - On Call" and the Job Number is 3029453 To find out more about the Brain Bank, you can also check out our website by searching for "Harvard Brain Bank". This will take you to our home page. I hope that everyone is having a wonderful holiday season. Tim Tim Wheelock Neuropathology Laboratory Harvard Brain Tissue Resource Center McLean Hospital, Belmont, MA 02478 Brain Bank general number: 1-800-272-4622 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From victor_tobias at comcast.net Sat Dec 31 08:57:54 2016 From: victor_tobias at comcast.net (victor_tobias at comcast.net) Date: Sat, 31 Dec 2016 06:57:54 -0800 Subject: [Histonet] Harvard Brain Bank Job Opportunity In-Reply-To: <69718C0B0B3C414D9F8E7214AD400CC99FCD4D9B@PHSX10MB11.partners.org> References: <69718C0B0B3C414D9F8E7214AD400CC99FCD4D9B@PHSX10MB11.partners.org> Message-ID: Tim, Congratulations, yesterday was my last day also. Victor Sent from Mail for Windows 10 From: Wheelock, Timothy R. via Histonet Sent: Friday, December 30, 2016 11:33 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Harvard Brain Bank Job Opportunity Hi Everyone: Well, at the age of 67 and after more than 30 years of service, I am retiring from the Harvard Brain Tissue Resource Center (the Brain Bank) at McLean Hospital in Belmont, Massachusetts. My last day is today, December 30th 2016. We are looking for someone to take over my position. If you are interested, please click on the following website. http://www.mcleanhospital.org/apply-work-mclean This website brings up the different positions currently open at McLean Hospital. The position is "Histologist Lab Manager - On Call" and the Job Number is 3029453 To find out more about the Brain Bank, you can also check out our website by searching for "Harvard Brain Bank". This will take you to our home page. I hope that everyone is having a wonderful holiday season. Tim Tim Wheelock Neuropathology Laboratory Harvard Brain Tissue Resource Center McLean Hospital, Belmont, MA 02478 Brain Bank general number: 1-800-272-4622 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet