[Histonet] Eosin

[Cameron, Elizabeth]

Hi,
I have been staining fish tissues fixed in Davidsons with H&E, and the researcher would like the eosin to be more intense.  Our standard protocol works well for our own tissue, but the fish look much more washed out.  I am using alcoholic eosin Y, have tried both water and alcohol before and I have varied the alcohol differentiation steps after the Eosin.  I also extended the time in Eosin and increased the wash after bluing to make sure the sections are not basic.  Any suggestions would be appreciated.
Thank you.

Elizabeth M. Cameron, HT(ASCP), QIHCCM
Lead Histologist
Mid Coast Hospital
123 Medical Center Drive
Brunswick, ME 04011
(207) 373-6573