From tabbott at pennstatehealth.psu.edu Mon Aug 1 08:15:56 2016 From: tabbott at pennstatehealth.psu.edu (Abbott, Tanya) Date: Mon, 1 Aug 2016 13:15:56 +0000 Subject: [Histonet] Medicare 14 day rule Message-ID: <56f1f3a664a044c3a4ebbe8e31ce9995@EXCHSRVR12.mshmc.local> I am just curious on how everyone is handling the Medicare 14 day rule when sending blocks out to reference labs for testing? Are you just holding on to those blocks for 14 days post discharge for inpatients then sending out? Reregistering these with the Date of Service as when you are sending these blocks out? Any input is appreciated! Best, Tanya Tanya G. Abbott Pathology Manager PennState Health St. Joseph Reading Pennsylvania From TNMayer at mdanderson.org Mon Aug 1 09:47:13 2016 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Mon, 1 Aug 2016 14:47:13 +0000 Subject: [Histonet] Block Scrapers Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC882822CD69@D1PWPEXMBX05.mdanderson.edu> Try DR Instruments for a decently priced knife, less than $10 each. They have the older thick, metal handled scalpel that we get for our students. They dull pretty quickly and are easy to keep up with. The thin ones can hurt your hands. They sell to the public, and I just googled them. Other than that try Buffalo Dental knives. It looks like a paring knife, but the blade is shorter (less than 1.5 in) and has a nice wooden handle. They are a more expensive, but last. Actually they are what dentists use to work on dentures. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 The information contained in the e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. Message: 1 Date: Mon, 25 Jul 2016 00:40:24 +0000 From: "Hardy, Cate" To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] block Scrapers Message-ID: Content-Type: text/plain; charset="utf-8" We just use a blunt butcher knife. It works great. Low tech, low cost! Cate Hardy Senior Technical Officer (Mon-Tue) | Veterinary Enterprises, Faculty of Science Charles Sturt University Boorooma Street Locked Bag 588 Wagga Wagga, NSW 2678 Australia Tel: 69334000 Email: vdl at csu.edu.au Email: chardy at csu.edu.au www.csu.edu.au The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From KSimeone at leavittmgt.com Mon Aug 1 12:34:34 2016 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Mon, 1 Aug 2016 17:34:34 +0000 Subject: [Histonet] FT OVERNIGHT HT POSTITION Delray Beach, FL In-Reply-To: <43944B1DBAAC2846B7B9D626B5F1233CC9194C6C@vm-email.leavittmgt.com> References: <43944B1DBAAC2846B7B9D626B5F1233CC9194C6C@vm-email.leavittmgt.com> Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CC91950B2@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed HISTOTECHNOLOGIST here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Please fill out employment application HERE http://www.indeed.com/m/viewjob?jk=266fccd1fa688bb6&from=serp ^^you MUST follow this application link to apply! No exceptions. *full time position Mon-Fri OR Sun-Thurs 10p-6:30AM *MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo most of your shift) *MUST have at LEAST FIVE (5) years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE! *VERY proficient in embedding and microtomy *WILL MOSTLY BE EMBEDDING EXCISION BLOCKS, please know DERMS *must be self motivated, reliable and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) *some IHC experience preferred but not necessary Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor Delray Beach Technical Laboratory ADCS Clinics, LLC Advanced Dermatology and Cosmetic Surgery Ameriderm www.advancedderm.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From SteveM at mcclainlab.com Mon Aug 1 12:56:49 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Mon, 1 Aug 2016 17:56:49 +0000 Subject: [Histonet] Histonet Digest, Vol 153, Issue 1 In-Reply-To: References: Message-ID: <83BE98DD-46A0-470F-9F99-6868B77068D8@mcclainlab.com> I agree w Dr. Richmond. > > Message: 2 > Date: Sun, 31 Jul 2016 17:43:20 -0400 > From: Bob Richmond > To: "Histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] B-5 Fixative > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Jillian A. Russell, HT (ASCP)CM, QIHCCM, Supervisor, CDx Histology > Operations, R&D at Dako in Carpinteria CA asks: > >>> I am wondering how many labs are using B-5 fixatives and how many are > using B-5 alternatives due to the mercury issue? - For those who have > switched to an alternative, have you noticed many differences from the > traditional B-5 fixative?<< > > This is a very controversial topic. B-5 fixative contains a large quantity > of mercuric chloride, along with formaldehyde, and you really can't use it > any more. I gave up B-5 and Zenker's fixatives very reluctantly. > > My personal opinion is that B-5 substitutes are no improvement on neutral > buffered formalin, and may compromise immunostains (which often specify NBF > fixation). > > Bob Richmond > From mfb.encarnacion at gmail.com Mon Aug 1 14:09:42 2016 From: mfb.encarnacion at gmail.com (Mary Faith Encarnacion) Date: Mon, 1 Aug 2016 12:09:42 -0700 Subject: [Histonet] On the hunt for new microtomes! Message-ID: Hey HistoNet, Thanks to everyone who helped me out by providing their opinions on embedding centers. This time, I need everyone's thoughts on microtomes. My lab is debating between the Thermo/Microm HM355S and the Leica RM2255. Your thoughts and advice are very much appreciated! If there are any more I should try, let me know! Thanks again, Mary Faith Histology Supervisor VA Palo Alto Health Care System From rjbuesa at yahoo.com Mon Aug 1 14:39:44 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 1 Aug 2016 19:39:44 +0000 (UTC) Subject: [Histonet] On the hunt for new microtomes! In-Reply-To: References: Message-ID: <1838985782.7452880.1470080384674.JavaMail.yahoo@mail.yahoo.com> I don't know now, but some years ago Thermo instruments were less that reliable. Try Leica or even better Sakura.Ren? On Monday, August 1, 2016 3:20 PM, Mary Faith Encarnacion via Histonet wrote: Hey HistoNet, Thanks to everyone who helped me out by providing their opinions on embedding centers.? This time, I need everyone's thoughts on microtomes. My lab is debating between the Thermo/Microm HM355S and the Leica RM2255. Your thoughts and advice are very much appreciated!? If there are any more I should try, let me know! Thanks again, Mary Faith Histology Supervisor VA Palo Alto Health Care System _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald at mtsac.edu Mon Aug 1 14:47:14 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Mon, 1 Aug 2016 12:47:14 -0700 Subject: [Histonet] On the hunt for new microtomes! In-Reply-To: <1838985782.7452880.1470080384674.JavaMail.yahoo@mail.yahoo.com> References: <1838985782.7452880.1470080384674.JavaMail.yahoo@mail.yahoo.com> Message-ID: Lecia makes the Sakura microtome. We have both Sakura and Microm microtomes in our student lab. They have both proved to be reliable. There is a difference between a Shandon microtome and a Microm microtome. From: Rene J Buesa via Histonet To: Mary Faith Encarnacion , "histonet at lists.utsouthwestern.edu" Date: 08/01/2016 12:42 PM Subject: Re: [Histonet] On the hunt for new microtomes! I don't know now, but some years ago Thermo instruments were less that reliable. Try Leica or even better Sakura.Ren? On Monday, August 1, 2016 3:20 PM, Mary Faith Encarnacion via Histonet wrote: Hey HistoNet, Thanks to everyone who helped me out by providing their opinions on embedding centers. This time, I need everyone's thoughts on microtomes. My lab is debating between the Thermo/Microm HM355S and the Leica RM2255. Your thoughts and advice are very much appreciated! If there are any more I should try, let me know! Thanks again, Mary Faith Histology Supervisor VA Palo Alto Health Care System _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper at chla.usc.edu Mon Aug 1 14:53:43 2016 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Mon, 1 Aug 2016 19:53:43 +0000 Subject: [Histonet] On the hunt for new microtomes! In-Reply-To: References: <1838985782.7452880.1470080384674.JavaMail.yahoo@mail.yahoo.com>, Message-ID: We've used Microm microtomes for years and they have always been 100% trouble free. -----Original Message----- From: Jennifer MacDonald via Histonet [histonet at lists.utsouthwestern.edu] Received: Monday, 01 Aug 2016, 12:45PM To: Rene J Buesa [rjbuesa at yahoo.com] CC: Mary Faith Encarnacion [mfb.encarnacion at gmail.com]; histonet at lists.utsouthwestern.edu [histonet at lists.utsouthwestern.edu] Subject: Re: [Histonet] On the hunt for new microtomes! Lecia makes the Sakura microtome. We have both Sakura and Microm microtomes in our student lab. They have both proved to be reliable. There is a difference between a Shandon microtome and a Microm microtome. From: Rene J Buesa via Histonet To: Mary Faith Encarnacion , "histonet at lists.utsouthwestern.edu" Date: 08/01/2016 12:42 PM Subject: Re: [Histonet] On the hunt for new microtomes! I don't know now, but some years ago Thermo instruments were less that reliable. Try Leica or even better Sakura.Ren? On Monday, August 1, 2016 3:20 PM, Mary Faith Encarnacion via Histonet wrote: Hey HistoNet, Thanks to everyone who helped me out by providing their opinions on embedding centers. This time, I need everyone's thoughts on microtomes. My lab is debating between the Thermo/Microm HM355S and the Leica RM2255. Your thoughts and advice are very much appreciated! If there are any more I should try, let me know! Thanks again, Mary Faith Histology Supervisor VA Palo Alto Health Care System _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From c.tague at Pathologyarts.com Mon Aug 1 16:14:59 2016 From: c.tague at Pathologyarts.com (Curt) Date: Mon, 1 Aug 2016 21:14:59 +0000 Subject: [Histonet] unsubscribe Message-ID: <9C8F910F72893643B3C3793C3D67132B67D23904@PATHOLOGYSERVER.pathologyarts.local> Thanks, CURT TAGUE | President/CEO PATHOLOGY ARTS | 1159 Pomona Road, Suite E, Corona, CA 92882 | 951.270.0605 Office 949.616.9911 Cell |http://www.pathologyarts.com/I c.tague at pathologyarts.com | From jaylundgren at gmail.com Mon Aug 1 17:13:39 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Mon, 1 Aug 2016 17:13:39 -0500 Subject: [Histonet] On the hunt for new microtomes! In-Reply-To: References: <1838985782.7452880.1470080384674.JavaMail.yahoo@mail.yahoo.com> Message-ID: Leica On Mon, Aug 1, 2016 at 2:53 PM, Cooper, Brian via Histonet < histonet at lists.utsouthwestern.edu> wrote: > We've used Microm microtomes for years and they have always been 100% > trouble free. > > -----Original Message----- > From: Jennifer MacDonald via Histonet [histonet at lists.utsouthwestern.edu] > Received: Monday, 01 Aug 2016, 12:45PM > To: Rene J Buesa [rjbuesa at yahoo.com] > CC: Mary Faith Encarnacion [mfb.encarnacion at gmail.com]; > histonet at lists.utsouthwestern.edu [histonet at lists.utsouthwestern.edu] > Subject: Re: [Histonet] On the hunt for new microtomes! > > Lecia makes the Sakura microtome. We have both Sakura and Microm > microtomes in our student lab. They have both proved to be reliable. > There is a difference between a Shandon microtome and a Microm microtome. > > > > From: Rene J Buesa via Histonet > To: Mary Faith Encarnacion , > "histonet at lists.utsouthwestern.edu" > Date: 08/01/2016 12:42 PM > Subject: Re: [Histonet] On the hunt for new microtomes! > > > > I don't know now, but some years ago Thermo instruments were less that > reliable. Try Leica or even better Sakura.Ren? > > On Monday, August 1, 2016 3:20 PM, Mary Faith Encarnacion via Histonet > wrote: > > > Hey HistoNet, > > Thanks to everyone who helped me out by providing their opinions on > embedding centers. This time, I need everyone's thoughts on microtomes. > My lab is debating between the Thermo/Microm HM355S and the Leica RM2255. > Your thoughts and advice are very much appreciated! If there are any more > I should try, let me know! > > Thanks again, > Mary Faith > Histology Supervisor > VA Palo Alto Health Care System > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, > please > contact the sender by reply e-mail and destroy all copies of this original > message. > > --------------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa at yahoo.com Tue Aug 2 09:43:16 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 2 Aug 2016 14:43:16 +0000 (UTC) Subject: [Histonet] On the hunt for new microtomes! In-Reply-To: <1838985782.7452880.1470080384674.JavaMail.yahoo@mail.yahoo.com> References: <1838985782.7452880.1470080384674.JavaMail.yahoo@mail.yahoo.com> Message-ID: <776097630.8251281.1470148996297.JavaMail.yahoo@mail.yahoo.com> As far as I know Leica Microsystems has bought/absorbed along the years many optical/instruments makers, such as Baush&Lomb, American Optical, Cambridge Instruments, Wild-Heerburg (Switzerland, although this one was a "reverse" acquisition), Australian "Bond" manufacturers, NOVOCASTRA laboratories?BUT I have never heard that it has bought Sakura instruments. It would be nice if somebody has reliable?information about this alleged acquisition.Ren?? On Monday, August 1, 2016 4:02 PM, Rene J Buesa via Histonet wrote: I don't know now, but some years ago Thermo instruments were less that reliable. Try Leica or even better Sakura.Ren? ? ? On Monday, August 1, 2016 3:20 PM, Mary Faith Encarnacion via Histonet wrote: Hey HistoNet, Thanks to everyone who helped me out by providing their opinions on embedding centers.? This time, I need everyone's thoughts on microtomes. My lab is debating between the Thermo/Microm HM355S and the Leica RM2255. Your thoughts and advice are very much appreciated!? If there are any more I should try, let me know! Thanks again, Mary Faith Histology Supervisor VA Palo Alto Health Care System _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald at mtsac.edu Tue Aug 2 10:07:21 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Tue, 2 Aug 2016 08:07:21 -0700 Subject: [Histonet] On the hunt for new microtomes! In-Reply-To: <776097630.8251281.1470148996297.JavaMail.yahoo@mail.yahoo.com> Message-ID: Your ignorant sarcasm isn't appreciated. Leica makes the microtome and private labels it for Sakura. Sent from my iPhone > On Aug 2, 2016, at 7:46 AM, Rene J Buesa via Histonet wrote: > > As far as I know Leica Microsystems has bought/absorbed along the years many optical/instruments makers, such as Baush&Lomb, American Optical, Cambridge Instruments, Wild-Heerburg (Switzerland, although this one was a "reverse" acquisition), Australian "Bond" manufacturers, NOVOCASTRA laboratories BUT I have never heard that it has bought Sakura instruments. It would be nice if somebody has reliable information about this alleged acquisition.Ren? > > On Monday, August 1, 2016 4:02 PM, Rene J Buesa via Histonet wrote: > > > I don't know now, but some years ago Thermo instruments were less that reliable. Try Leica or even better Sakura.Ren? > > On Monday, August 1, 2016 3:20 PM, Mary Faith Encarnacion via Histonet wrote: > > > Hey HistoNet, > > Thanks to everyone who helped me out by providing their opinions on > embedding centers. This time, I need everyone's thoughts on microtomes. > My lab is debating between the Thermo/Microm HM355S and the Leica RM2255. > Your thoughts and advice are very much appreciated! If there are any more > I should try, let me know! > > Thanks again, > Mary Faith > Histology Supervisor > VA Palo Alto Health Care System > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From naje1972 at yahoo.com Tue Aug 2 13:49:17 2016 From: naje1972 at yahoo.com (cynthia haynes) Date: Tue, 2 Aug 2016 18:49:17 +0000 (UTC) Subject: [Histonet] Wax clogs in VIP 6 processor References: <1833276652.9872747.1470163757288.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1833276652.9872747.1470163757288.JavaMail.yahoo@mail.yahoo.com> Hello Histo NetI am having a problem with wax clogs on my VIP 6 processor. This unit vents to the outside and I was just wondering if that may?have something to do with my problem. It started 2 weeks ago. I've had the unit serviced?2 x's. It has been very humid in our city. I am?changing sol'n regularly as per company supplied maintenance program. HELP!. I am at my wits end. This relatively a new unit. 3 year old. Has anyone experience this problem before.Thanks in advance.Cynthia Haynes-James H.T. From SteveM at mcclainlab.com Tue Aug 2 13:56:51 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Tue, 2 Aug 2016 18:56:51 +0000 Subject: [Histonet] Histonet Digest, Vol 153, Issue 2 microtomes In-Reply-To: References: Message-ID: Depends on how long you plan to be in business and whether you are using other people's money. But buy all the same model and make if you can. I've owned the same Leica 2125 and 2135 microtomes for 12 years. We usually cut mainly small biopsies but have pushed those to occasionally cut large research blocks up to 45mm by 60mm. I cannot speak to other models. Since I've owned Leica, I haven't had any other experience. Like all mechanical equipment, maintenance is critical or none will cut properly. Steve A. McClain, MD > On Aug 2, 2016, at 13:19, "histonet-request at lists.utsouthwestern.edu" wrote: > > Thanks to everyone who helped me out by providing their opinions on > embedding centers. This time, I need everyone's thoughts on microtomes. > My lab is debating between the Thermo/Microm HM355S and the Leica RM2255. > Your thoughts and advice are very much appreciated! If there are any more > I should try, let me know! > > Thanks again, > Mary Faith From AJohnson at aipathology.com Wed Aug 3 09:06:09 2016 From: AJohnson at aipathology.com (Amy Johnson) Date: Wed, 3 Aug 2016 14:06:09 +0000 Subject: [Histonet] slides on the Benchmark Ultra Message-ID: Hello Histonetters, WE currently run the Benchmark Ultra for our IHC staining. We have been using SuperFrost Plus slides on the recommendation by Ventana. What are others using if you aren't using SuperFrost Plus slides. Our vendor that we get SuperFrost Plus from no longer carries them and has replaced them with another type......Just wondering what others think Thanks Amylin Johnson, B.S. HTL(ASCP) Associates in Pathology Wausau Wi 54401 715-847-2130 From SteveM at mcclainlab.com Wed Aug 3 12:56:11 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Wed, 3 Aug 2016 17:56:11 +0000 Subject: [Histonet] Histonet Digest, Vol 153, Issue 3 In-Reply-To: References: Message-ID: <7B140852-8E85-4596-ACF6-08D7A9B0466F@mcclainlab.com> Never had one of fancy new ones! But I have one V5 and 4 K Sakuras. Those back to the future Delorean stainless steel models run circles around the already rusting needs to be painted 8 year old model down more in its short life than all 4 flux capacitor Ks put together. Start by analyzing your history. What tissue tissues are you processing? How long is your cycle? What clearant-xylene? How long is your cleaning cycle? What tissue wrapping are you using? Steve A. McClain, MD > On Aug 3, 2016, at 13:15, "histonet-request at lists.utsouthwestern.edu" wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Wax clogs in VIP 6 processor (cynthia haynes) > 2. Re: Histonet Digest, Vol 153, Issue 2 microtomes (Steve McClain) > 3. slides on the Benchmark Ultra (Amy Johnson) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 2 Aug 2016 18:49:17 +0000 (UTC) > From: cynthia haynes > To: Histonet > Subject: [Histonet] Wax clogs in VIP 6 processor > Message-ID: > <1833276652.9872747.1470163757288.JavaMail.yahoo at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > Hello Histo NetI am having a problem with wax clogs on my VIP 6 processor. This unit vents to the outside and I was just wondering if that may?have something to do with my problem. It started 2 weeks ago. I've had the unit serviced?2 x's. It has been very humid in our city. I am?changing sol'n regularly as per company supplied maintenance program. HELP!. I am at my wits end. This relatively a new unit. 3 year old. Has anyone experience this problem before.Thanks in advance.Cynthia Haynes-James H.T. > > ------------------------------ > > Message: 2 > Date: Tue, 2 Aug 2016 18:56:51 +0000 > From: Steve McClain > To: "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Histonet Digest, Vol 153, Issue 2 microtomes > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Depends on how long you plan to be in business and whether you are using other people's money. But buy all the same model and make if you can. > > I've owned the same Leica 2125 and 2135 microtomes for 12 years. > We usually cut mainly small biopsies but have pushed those to occasionally cut large research blocks up to 45mm by 60mm. > I cannot speak to other models. Since I've owned Leica, I haven't had any other experience. > > Like all mechanical equipment, maintenance is critical or none will cut properly. > Steve A. McClain, MD > >> On Aug 2, 2016, at 13:19, "histonet-request at lists.utsouthwestern.edu" wrote: >> >> Thanks to everyone who helped me out by providing their opinions on >> embedding centers. This time, I need everyone's thoughts on microtomes. >> My lab is debating between the Thermo/Microm HM355S and the Leica RM2255. >> Your thoughts and advice are very much appreciated! If there are any more >> I should try, let me know! >> >> Thanks again, >> Mary Faith > > > > ------------------------------ > > Message: 3 > Date: Wed, 3 Aug 2016 14:06:09 +0000 > From: Amy Johnson > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] slides on the Benchmark Ultra > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hello Histonetters, > > WE currently run the Benchmark Ultra for our IHC staining. We have been using SuperFrost Plus slides on the recommendation by Ventana. What are others using if you aren't using SuperFrost Plus slides. > Our vendor that we get SuperFrost Plus from no longer carries them and has replaced them with another type......Just wondering what others think > > > Thanks > Amylin Johnson, B.S. HTL(ASCP) > Associates in Pathology > Wausau Wi 54401 > 715-847-2130 > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 153, Issue 3 > **************************************** From ADuddey at firsthealth.org Wed Aug 3 13:55:51 2016 From: ADuddey at firsthealth.org (Duddey, Aimee) Date: Wed, 3 Aug 2016 18:55:51 +0000 Subject: [Histonet] slides on the Benchmark Ultra In-Reply-To: References: Message-ID: <09FBA01CA9B6374A83C5C76E09E46188823E7843@EXMAIL1-FHC.firsthealth.org> We use super frost plus and have tried many different slides over the last 15 years. We always go back to SuperFrost plus. Cardinal Healthcare carries them under their brand but they are made by Erie Scientific. It is my understanding that there are very few companies that make slides, they are just branded differently. Check the manufacturer and if its Erie Scientific and they are SuperFrost then they should work as well. Aimee M. Duddey, MLT(ASCP) Assistant Director of Laboratory - Pathology FH Moore Regional Hospital 910-715-5286 office 910715-1944 fax -----Original Message----- From: Amy Johnson via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, August 03, 2016 10:06 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] slides on the Benchmark Ultra Hello Histonetters, WE currently run the Benchmark Ultra for our IHC staining. We have been using SuperFrost Plus slides on the recommendation by Ventana. What are others using if you aren't using SuperFrost Plus slides. Our vendor that we get SuperFrost Plus from no longer carries them and has replaced them with another type......Just wondering what others think Thanks Amylin Johnson, B.S. HTL(ASCP) Associates in Pathology Wausau Wi 54401 715-847-2130 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From edmartin26 at gmail.com Wed Aug 3 14:07:15 2016 From: edmartin26 at gmail.com (Eddie Martin) Date: Wed, 3 Aug 2016 14:07:15 -0500 Subject: [Histonet] slides on the Benchmark Ultra In-Reply-To: References: Message-ID: Hi Amy, While I worked at the University of Alabama in Birmingham, they used the super frost plus slides provided through Cardinal Health. They were the same Fisher slides to be used on the Ventana IHC analyzers. I hope this helps. Best, Eddie Martin, HT(ASCP),QIHC,HTL Sent from my iPhone > On Aug 3, 2016, at 9:06 AM, Amy Johnson wrote: > > Hello Histonetters, > > WE currently run the Benchmark Ultra for our IHC staining. We have been using SuperFrost Plus slides on the recommendation by Ventana. What are others using if you aren't using SuperFrost Plus slides. > Our vendor that we get SuperFrost Plus from no longer carries them and has replaced them with another type......Just wondering what others think > > > Thanks > Amylin Johnson, B.S. HTL(ASCP) > Associates in Pathology > Wausau Wi 54401 > 715-847-2130 > > From patpxs at gmail.com Wed Aug 3 14:53:41 2016 From: patpxs at gmail.com (P Sicurello) Date: Wed, 3 Aug 2016 12:53:41 -0700 Subject: [Histonet] CPT code for indirect IF Message-ID: Good Afternoon Netters, Is there a CPT code to use for indirect IF? I see the one for direct, but I'm not sure if the CPT code book labels indirect IF as multiplex testing. How do you all code for the indirect IF? Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From AJohnson at aipathology.com Thu Aug 4 06:17:16 2016 From: AJohnson at aipathology.com (Amy Johnson) Date: Thu, 4 Aug 2016 11:17:16 +0000 Subject: [Histonet] another question about superfrost plus slides Message-ID: Hello again, If you used a certain kind of slide (superfrost plus) and then switch to a different kind of slide (millennia 2.0) is it necessary to revalidate all of your IHC antibodies? Thanks again Amylin Johnson, B.S. HTL(ASCP) Associates in Pathology Wausau Wi 54401 715-847-2130 From mcginnessjamie at yahoo.com Thu Aug 4 17:50:06 2016 From: mcginnessjamie at yahoo.com (Jamie McGinness) Date: Thu, 4 Aug 2016 17:50:06 -0500 Subject: [Histonet] Cryojane solution A Message-ID: I am making my own cryojane slides. I am wondering if anyone knows the importance and the reason for slide pretreatment with solution A prior to solution B. From craigak12 at gmail.com Thu Aug 4 18:44:13 2016 From: craigak12 at gmail.com (J B) Date: Thu, 04 Aug 2016 23:44:13 +0000 Subject: [Histonet] QAQC SOP: Message-ID: Is anyone willing to share a procedure for QAQC? I have a procedure that is extremely detailed and our physicans would like a shorter version. I would love to see what others have in practice if you are willing to share it is greatly appreciated. Sincerely, JB From gentras at auburn.edu Fri Aug 5 10:48:42 2016 From: gentras at auburn.edu (Atoska Gentry) Date: Fri, 5 Aug 2016 15:48:42 +0000 Subject: [Histonet] Automated Slide Stainers Message-ID: Hello, one of my department's researchers is in need of an automated slide stainer which accommodates 3"x2" microscope slides. If you have experience with such will you please contact me at your earliest convenience? Regards, Atoska Gentry From liz at premierlab.com Fri Aug 5 11:18:37 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Fri, 5 Aug 2016 10:18:37 -0600 Subject: [Histonet] Automated Slide Stainers In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02BED535B589@SBS2K8.premierlab.local> Atoska I'm pretty sure that the Sakura Tissue Tek Prisma has the capability to hold 2 x 3 slides with some adaptors to the slide baskets, I have a fax from 2009 that list the parts necessary. The regular 20 slide holders can be changed to hold 5 - 2 x 3 slides. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Atoska Gentry via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, August 05, 2016 9:49 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Automated Slide Stainers Hello, one of my department's researchers is in need of an automated slide stainer which accommodates 3"x2" microscope slides. If you have experience with such will you please contact me at your earliest convenience? Regards, Atoska Gentry _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lmarie08 at uga.edu Fri Aug 5 13:02:51 2016 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Fri, 5 Aug 2016 18:02:51 +0000 Subject: [Histonet] chicken brain Message-ID: Hello Histonet world, I have been charged with the task of opening a week and a half old chicken head to retrieve its brain for Histo. Anyone out there done this before and if so, what instruments did you use? Thanks! L From jaylundgren at gmail.com Fri Aug 5 17:46:32 2016 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 5 Aug 2016 17:46:32 -0500 Subject: [Histonet] Genomic Health? Message-ID: Anyone from Genomic Health in Redwood City, Ca on here? Thanks, Jay A. Lundgren, M.S., HTL (ASCP) From akbitting at geisinger.edu Mon Aug 8 09:00:47 2016 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Mon, 8 Aug 2016 14:00:47 +0000 Subject: [Histonet] [External] Re: Automated Slide Stainers In-Reply-To: References: Message-ID: <2dbefd16c27340189d065ba23d986437@LOFEXMBX108W12V.geisinger.edu> There are slide basket inserts to accommodate that sized slide for Sakura stainers. The DRS2000 for example. Check out this web page. http://www.tedpella.com/histo_html/slides-large.htm -----Original Message----- From: Atoska Gentry via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, August 05, 2016 11:49 AM To: histonet at lists.utsouthwestern.edu Subject: [External] Re: [Histonet] Automated Slide Stainers Hello, one of my department's researchers is in need of an automated slide stainer which accommodates 3"x2" microscope slides. If you have experience with such will you please contact me at your earliest convenience? Regards, Atoska Gentry _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3a%2f%2flists.utsouthwestern.edu%2fmailman%2flistinfo%2fhistonet&data=01%7c01%7cakbitting%40geisinger.edu%7c885f9438ed16490e655308d3bd4817ee%7c37d46c567c664402a16055c2313b910d%7c0&sdata=OOfSjJcD5xJbEjjKsWItiOmbHVv9p8V8nkt3B64PAgk%3d IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From jvickroy at SpringfieldClinic.com Mon Aug 8 15:23:21 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Mon, 8 Aug 2016 20:23:21 +0000 Subject: [Histonet] Intraoperative tissue requisition or form Message-ID: <9B1A1501A800064397369BD8072E6BCA06594B45@E2K10DB.springfieldclinic.com> We are a physician owned clinic and the number of frozen sections we do is small. Occasionally we have a few skin biopsies that are done in the ASC surgery department. I got a call from them today and they have a problem that they encounter every time they do a frozen section. When the surgeon takes the biopsy a nurse has to hold the specimen in surgery until the tissue order (requisition) is put into the computer. With staffing in ASC this may take fifteen minutes after the biopsy is removed and given to the nurse, especially if the surgeon is asking the nurse to do other things in the meantime. Of course when the specimen then is received by Histology it may take another 15-20 minutes before the surgeon gets a frozen section diagnosis. This morning the surgeon said this needs to get fixed. Of course it's easy for me to say to the ASC department that someone needs to put in the information immediately and not take fifteen minutes. I even asked why can't the order be put into the computer before the frozen is done. My other response to ASC was that we can do the frozen section but we can't assign a number not print barcoded slides and blocks until the order has been entered into the computer. I do recall that organizations with less robust IT systems than CoPath sometimes use a written Frozen section order form and report. Can anyone share with me a form they use currently that I could modify for our purposes? I know the basics but don't want to have to reinvent the wheel if someone already has a form that they use for this purpose. Of course once the nurses have had time to put the order into the computer histology would have to enter the case into the pathology system, assigning a surgical number. The pathologist would have to in turn take the diagnosis etc. off of the written sheet and enter the diagnosis and gross into the computer system. The written form would in turn have to be kept for documentation which can include scanning into the electronic health record and would include things like time surgeon is called, site, patient sticker with patient identifiers, clinical history, etc. I realize that this might seem like a step backward however given staffing issues I understand where the ASC nurse manager is coming from and if it helps the patient and surgeon I think we can live with the inconvenience. Your thoughts and examples of forms or frozen section requisitions? Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From paula at excaliburpathology.com Mon Aug 8 17:52:43 2016 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Mon, 8 Aug 2016 22:52:43 +0000 (UTC) Subject: [Histonet] Pan Cytokeratin References: <433712304.1847321.1470696763351.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <433712304.1847321.1470696763351.JavaMail.yahoo@mail.yahoo.com> Hello, would anyone be willing to donate a recently expired, about to be tossed, vial of pan cytokeratin for a research project? I will pay shipping. Thank you,?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From Fawn.Bomar at HalifaxRegional.com Tue Aug 9 05:06:13 2016 From: Fawn.Bomar at HalifaxRegional.com (Fawn Bomar) Date: Tue, 9 Aug 2016 10:06:13 +0000 Subject: [Histonet] Decon Processor Message-ID: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA1FEF79@EXCH-2K10.hrhs.com> Hi everyone, Our lab is getting ready to install a new processor. My question is, how do we go about decontaminating our old processor to dispose of it. The old processor is a Ventana Rennaissance. Thank you Fawn Bomar ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From Stephen.Clark1 at hcahealthcare.com Tue Aug 9 06:19:34 2016 From: Stephen.Clark1 at hcahealthcare.com (Stephen.Clark1 at hcahealthcare.com) Date: Tue, 9 Aug 2016 11:19:34 +0000 Subject: [Histonet] Pathology Ladder System Message-ID: Good morning everyone. I had a meeting recently with hospital administration where I was asked what leadership could do to retain and keep quality staff. Besides the obvious, pay increase's, I suggested a ladder type progression system for all lab staff, similar to what some hospitals offer nursing staff. My question to this group is, if any of you are using such a system would you mind sharing it with me? Thanks, Stephen Clark HT, BS, DESSO Pathology Dept. Supervisor Laboratory Safety Officer Grand Strand Medical Center 809 82nd Parkway Myrtle Beach, SC 29579 Desk: 843-692-1459 Lab: 843-692-1486 Fax: 843-449-6213 From ADuddey at firsthealth.org Tue Aug 9 07:21:14 2016 From: ADuddey at firsthealth.org (Duddey, Aimee) Date: Tue, 9 Aug 2016 12:21:14 +0000 Subject: [Histonet] Pathology Ladder System In-Reply-To: References: Message-ID: <09FBA01CA9B6374A83C5C76E09E46188823EB11D@EXMAIL1-FHC.firsthealth.org> I have had the same discussion with lab administration recently. I would be interested in the same information. Aimee -----Original Message----- From: Steve via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 09, 2016 7:20 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pathology Ladder System Good morning everyone. I had a meeting recently with hospital administration where I was asked what leadership could do to retain and keep quality staff. Besides the obvious, pay increase's, I suggested a ladder type progression system for all lab staff, similar to what some hospitals offer nursing staff. My question to this group is, if any of you are using such a system would you mind sharing it with me? Thanks, Stephen Clark HT, BS, DESSO Pathology Dept. Supervisor Laboratory Safety Officer Grand Strand Medical Center 809 82nd Parkway Myrtle Beach, SC 29579 Desk: 843-692-1459 Lab: 843-692-1486 Fax: 843-449-6213 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From taylor at prometheushealthcare.com Tue Aug 9 07:39:52 2016 From: taylor at prometheushealthcare.com (Taylor Rinaldi) Date: Tue, 9 Aug 2016 08:39:52 -0400 Subject: [Histonet] **Histology Job Opportunities** Message-ID: <18bd01d1f23b$212ffb80$638ff280$@prometheushealthcare.com> Hi there! My name is Taylor Rinaldi, Recruiting Manager at Prometheus healthcare. My team and I specialize specifically in laboratory recruiting nationwide, working directly with lab managers from different hospitals and reference laboratories across the US . We are currently recruiting for multiple Histotechnologist and Histotechnician opportunities. All the opportunities are fulltime, and permanent. ASCP certification is preferred but not required for all. If you or any of your colleagues have been considering a new position in the Histology field, please don't hesitate to reach out to me directly for immediate referral and submittal to some of the top hospitals and labs nationwide. Current states: Chattanooga, Tennessee Sacramento, California New York, New York Atlanta, Georgia Dallas, Texas Thank you all in advance, Taylor Rinaldi Recruiting Manager Prometheus Healthcare Office 866-857-1434 Taylor at prometheushealthcare.com From brett_connolly at merck.com Tue Aug 9 08:57:41 2016 From: brett_connolly at merck.com (Connolly, Brett M) Date: Tue, 9 Aug 2016 09:57:41 -0400 Subject: [Histonet] JOH journal collection Message-ID: HI all, Is anyone interested in old issues of JOH? My collection spans 1997 - 2016. I am missing a few (~ 7). Let me know and I'll send you a list of what I have. Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From amurvosh at advancederm.net Tue Aug 9 08:56:31 2016 From: amurvosh at advancederm.net (Anne Murvosh) Date: Tue, 9 Aug 2016 13:56:31 +0000 Subject: [Histonet] Pathology Ladder System In-Reply-To: References: Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7B34236@Exchange.Advancederm.net> If you don't already have it you may want to offer a shift differential for very early or late shifts and weekends. This makes it a little easier to hire for and keep people on the harder shifts. Anne -----Original Message----- From: Steve via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 09, 2016 4:20 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pathology Ladder System Good morning everyone. I had a meeting recently with hospital administration where I was asked what leadership could do to retain and keep quality staff. Besides the obvious, pay increase's, I suggested a ladder type progression system for all lab staff, similar to what some hospitals offer nursing staff. My question to this group is, if any of you are using such a system would you mind sharing it with me? Thanks, Stephen Clark HT, BS, DESSO Pathology Dept. Supervisor Laboratory Safety Officer Grand Strand Medical Center 809 82nd Parkway Myrtle Beach, SC 29579 Desk: 843-692-1459 Lab: 843-692-1486 Fax: 843-449-6213 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Tue Aug 9 11:10:27 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 9 Aug 2016 16:10:27 +0000 Subject: [Histonet] Pathology Ladder System In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF6FD7B06C@ex07.net.ucsf.edu> Our career ladder at UCSF HLT 1 thru 4 (Hospital Laboratory Technician) for non-histology positions. HLT-1 is entry level, could be out of high school. To move up requires more education. HLT-2 AA to BA/BA, HLT-3, -4, BA/BS used as accessioners, basic lab techs in grossing and cytology, running stainers, changing tissue processors in histology, etc. HLT-1,-2 are bench techs. HLT-3 is a Senior tech, HLT-4 is a Lead tech. HLT-3 and -4 can be trained for grossing. They can actually work their way thru to minor grossing with the right qualifications. If trained for histotechnology or more advanced grossing ie, PA-level) we promote them to the Histotechnologist series. HT 1 thru 4 (Histotechnologist). Traditional histology lab techs as well as on-the-job trained Pathologist's Assistants (HT-3,-4 only). HT-1,2 are the basic bench techs, HT-3, is Senior tech, HT-4 is Lead tech. Only HT-4 and Supervisor requires ASCP certification for histology work (not grossing work), but is "preferred" at the other levels. So far all our histotechs are ASCP certified HT or HTL. None of the OJT PA's are certified (though they could work for it if they desired). We do have an advantage of a large research population to draw techs from so all come to us with good educational background and have learned some histology in a research lab. These are all union positions and each classification (i.e HT-1 or HT-2) has 12 annual steps (controlled by the union, not administration) before maxing out in pay. At that point they reach a ceiling and can only move up by going to the next up classification. We usually require 5 years in one step before reclassifying unless the person is exceptional. As a manager I can only reclassify; I cannot move someone up a step as a reward for good work. This works out pretty well for advancement but we have a fairly large organization - 12 histotechs and 2 HLT in the histo lab; 4 HLT in cyto lab; 4 certifed PA's; 4 OJT PA's plus 5 HLT accessioners - moving up or to other positions within the department is possible. Several of our accessioners have moved up to Histotech positions, and one OJT PA went to PA school and came back to work for us. Another HLT accessioner is applying to PA school. We also now hire only certified PA's, and try to hire program-trained PA's. the competition for that is tough and for recent positions we have essentially opened it to any certified PA. Cyto uses the HLT series for lab work and has another Cytotechnologist series for cytotechs, same idea of level 1-4. At the top of each lab is the supervisor in each discipline, one for cytology, histology, grossing and administration. The usual problem in Histo labs is that people stay a long time and so block others from moving up, so even if a career ladder is in place advancement is difficult. Unless the department expands the opportunities to advance are limited in many labs. I histology we have had quite a lot of turnover the last 5 years because we had a 4 histotechs retire within a couple years so some advanced and some new techs came in. We also opened a couple new jobs. So we now have a fairly young lab with a lot of very good techs. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Steve via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 09, 2016 4:20 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pathology Ladder System Good morning everyone. I had a meeting recently with hospital administration where I was asked what leadership could do to retain and keep quality staff. Besides the obvious, pay increase's, I suggested a ladder type progression system for all lab staff, similar to what some hospitals offer nursing staff. My question to this group is, if any of you are using such a system would you mind sharing it with me? Thanks, Stephen Clark HT, BS, DESSO Pathology Dept. Supervisor Laboratory Safety Officer Grand Strand Medical Center 809 82nd Parkway Myrtle Beach, SC 29579 Desk: 843-692-1459 Lab: 843-692-1486 Fax: 843-449-6213 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From craigak12 at gmail.com Tue Aug 9 12:33:48 2016 From: craigak12 at gmail.com (J B) Date: Tue, 09 Aug 2016 17:33:48 +0000 Subject: [Histonet] QAQC Procedure: Message-ID: Is anyone willing to share a procedure for QAQC? I have a procedure that is extremely detailed and our physicans would like a shorter version. I would love to see what others have in practice if you are willing to share it is greatly appreciated. Sincerely, JB From kkienitz at orclinic.com Tue Aug 9 12:41:31 2016 From: kkienitz at orclinic.com (Kienitz, Kari) Date: Tue, 9 Aug 2016 17:41:31 +0000 Subject: [Histonet] productivity standards/slide Message-ID: Hi everyone, What constitutes a slide? For volume purposes/tech productivity..... 1 section on a slide= 1 slide 3 sections (levels) on a slide=1 slide or does it equal 3 slides? Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz at orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you From Timothy.Morken at ucsf.edu Tue Aug 9 13:03:16 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 9 Aug 2016 18:03:16 +0000 Subject: [Histonet] productivity standards/slide In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF6FD7B179@ex07.net.ucsf.edu> That's a good question, but tough to answer because every lab has different ways of mounting sections. I think you will have to define that for your own workflow. I've seen labs that require 30 serial sections on a slide for certain specimens, so what is that?! You could start with the one section/one slide as the base line, then determine how much extra time it takes to mount levels (cutting between sections taken), or other types of mounting. Then you can apply a multiplier to those types of slides and come up with a reasonable productivity guideline. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Kienitz, Kari via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 09, 2016 10:42 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] productivity standards/slide Hi everyone, What constitutes a slide? For volume purposes/tech productivity..... 1 section on a slide= 1 slide 3 sections (levels) on a slide=1 slide or does it equal 3 slides? Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz at orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jo-ann.bader at mcgill.ca Tue Aug 9 14:02:48 2016 From: jo-ann.bader at mcgill.ca (Jo-Ann Bader, Ms.) Date: Tue, 9 Aug 2016 19:02:48 +0000 Subject: [Histonet] Cryostat sectioning of frozen leaves Message-ID: I am trying to cut frozen leaf sections at 6 and 8 microns. All I am getting is bunched up leaf. What is the trick/ I am cutting at -20 should be cutting at a lower temp and cutting thicker? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 From Johanna.Haddock at propath.com Tue Aug 9 14:27:08 2016 From: Johanna.Haddock at propath.com (Johanna Haddock) Date: Tue, 9 Aug 2016 19:27:08 +0000 Subject: [Histonet] FW: Microm HM 505 E Cryostat Message-ID: <5FA89F1EC1BB7C4A9B4AD8678D3E0CA83E879AED@Mail.propathlab.com> Hi, Does anyone has the Manufacturer Instruction Manual for the Microm HM 505 E Cryostat that you can share with me? Thanks, Johanna Haddock, BS, MT(ASCP) QA Coordinator ProPath - The Leader in Pathology Services 1355 River Bend Dr. Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPath.com " This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From Johanna.Haddock at propath.com Tue Aug 9 14:48:14 2016 From: Johanna.Haddock at propath.com (Johanna Haddock) Date: Tue, 9 Aug 2016 19:48:14 +0000 Subject: [Histonet] Microm HM 505 E Cryostat Message-ID: <5FA89F1EC1BB7C4A9B4AD8678D3E0CA83E879B57@Mail.propathlab.com> Thank you very much. I received a copy of the document. Johanna Haddock, BS, MT(ASCP) QA Coordinator ProPath - The Leader in Pathology Services 1355 River Bend Dr. Dallas, TX 75247 214-237-1623 214-237-1731 Fax To learn more about ProPath, please visit http://www.ProPath.com " This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message". From: Johanna Haddock Sent: Tuesday, August 09, 2016 2:27 PM To: histonet at lists.utsouthwestern.edu Subject: FW: Microm HM 505 E Cryostat Hi, Does anyone has the Manufacturer Instruction Manual for the Microm HM 505 E Cryostat that you can share with me? Thanks, Johanna Haddock, BS, MT(ASCP) QA Coordinator ProPath - The Leader in Pathology Services 1355 River Bend Dr. Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPath.com " This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From BDUE at PARTNERS.ORG Tue Aug 9 15:32:36 2016 From: BDUE at PARTNERS.ORG (Due, Brice) Date: Tue, 9 Aug 2016 20:32:36 +0000 Subject: [Histonet] PFA Fixed tissue not sticking to slides? Message-ID: Hello Jennifer, yes over-fixation will cause adhesion problems. This applies to formalin fixed frozens as well as PFA fixed frozens. I have had good luck using chromated gelatin to coat slides. I have successfully cut and stained lung specimens left in NBF for several months. Commercially, chromated gelatin is sold as STA-ON. I believe Leica now owns it, but you can make your own quite easily. I tend to use STA-ON full strength, or minimally diluted. Spread some on a slide with a transfer pipette, taking care not to allow it to run onto the back of the slide. Shake off the excess and set vertical to dry. The coating MUST be dry before you use the slide to pick up sections. Freshly made slides work best. After pick up, it is important to air-dry the section completely before immersing it in any solution. The moisture content of the frozen section must evaporate or the chromated gelatin will not hold the tissue. Don't worry, fixed sections do not suffer from artifacts when air-dried because the morphology is already locked by the fixation. Best luck! -brice The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From histo at pathlab.us Wed Aug 10 09:09:17 2016 From: histo at pathlab.us (Histology) Date: Wed, 10 Aug 2016 14:09:17 +0000 Subject: [Histonet] PGP 9.5 at 50 microns Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD824A3E51@vdc01.domain.local> Hi All, I need help. My doctors want to start doing some diabetic nerve testing. They need a PGP 9.5 IHC done on a frozen section cut at 50 microns. Does anybody have any experience on something like this? If so, I would love some help. Thanks, Mehndi Helgren Dominion Pathology Labs From TNMayer at mdanderson.org Wed Aug 10 11:17:26 2016 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Wed, 10 Aug 2016 16:17:26 +0000 Subject: [Histonet] Pathology Ladder System Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC8828235F6E@D1PWPEXMBX05.mdanderson.edu> Stephen, This question is being asked all over the country. Your suggestion of having a clear cut career ladder is good. Add to that the opportunity to cross train in other areas such as POCT testing, molecular, and digital imaging. Today's techs want opportunities to advance and most of them don't mind learning new things, they just want to be compensated for that. New techs don't mind moving around some to get that dream job, but they need a definite track to do so. Mentoring in other areas is good as well. Allow them the chance to participate in other administrative areas so that they can see what is out there. Rotate these spots so that everyone can get a chance if they want. I see you are the safety person, send someone else to your meeting next time. NSH is also good for investigating and networking. We actually just discussed this with our department earlier this summer, histotechs want a career ladder to see what is out there. They don't want to wait 10 years to get a good position, so they job hop. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 The information contained in the e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. -----Original Message----- Message: 4 Date: Tue, 9 Aug 2016 11:19:34 +0000 From: To: Subject: [Histonet] Pathology Ladder System Message-ID: Content-Type: text/plain; charset="us-ascii" Good morning everyone. I had a meeting recently with hospital administration where I was asked what leadership could do to retain and keep quality staff. Besides the obvious, pay increase's, I suggested a ladder type progression system for all lab staff, similar to what some hospitals offer nursing staff. My question to this group is, if any of you are using such a system would you mind sharing it with me? Thanks, Stephen Clark HT, BS, DESSO Pathology Dept. Supervisor Laboratory Safety Officer Grand Strand Medical Center 809 82nd Parkway Myrtle Beach, SC 29579 Desk: 843-692-1459 Lab: 843-692-1486 Fax: 843-449-6213 ------------------------------ Message: 9 Date: Tue, 9 Aug 2016 16:10:27 +0000 From: "Morken, Timothy" To: Histonet Subject: Re: [Histonet] Pathology Ladder System Message-ID: <761E2B5697F795489C8710BCC72141FF6FD7B06C at ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Our career ladder at UCSF HLT 1 thru 4 (Hospital Laboratory Technician) for non-histology positions. HLT-1 is entry level, could be out of high school. To move up requires more education. HLT-2 AA to BA/BA, HLT-3, -4, BA/BS used as accessioners, basic lab techs in grossing and cytology, running stainers, changing tissue processors in histology, etc. HLT-1,-2 are bench techs. HLT-3 is a Senior tech, HLT-4 is a Lead tech. HLT-3 and -4 can be trained for grossing. They can actually work their way thru to minor grossing with the right qualifications. If trained for histotechnology or more advanced grossing ie, PA-level) we promote them to the Histotechnologist series. HT 1 thru 4 (Histotechnologist). Traditional histology lab techs as well as on-the-job trained Pathologist's Assistants (HT-3,-4 only). HT-1,2 are the basic bench techs, HT-3, is Senior tech, HT-4 is Lead tech. Only HT-4 and Supervisor requires ASCP certification for histology work (not grossing work), but is "preferred" at the other levels. So far all our histotechs are ASCP certified HT or HTL. None of the OJT PA's are certified (though they could work for it if they desired). We do have an advantage of a large research population to draw techs from so all come to us with good educational background and have learned some histology in a research lab. These are all union positions and each classification (i.e HT-1 or HT-2) has 12 annual steps (controlled by the union, not administration) before maxing out in pay. At that point they reach a ceiling and can only move up by going to the next up classification. We usually require 5 years in one step before reclassifying unless the person is exceptional. As a manager I can only reclassify; I cannot move someone up a step as a reward for good work. This works out pretty well for advancement but we have a fairly large organization - 12 histotechs and 2 HLT in the histo lab; 4 HLT in cyto lab; 4 certifed PA's; 4 OJT PA's plus 5 HLT accessioners - moving up or to other positions within the department is possible. Several of our accessioners have moved up to Histotech positions, and one OJT PA went to PA school and came back to work for us. Another HLT accessioner is applying to PA school. We also now hire only certified PA's, and try to hire program-trained PA's. the competition for that is tough and for recent positions we have essentially opened it to any certified PA. Cyto uses the HLT series for lab work and has another Cytotechnologist series for cytotechs, same idea of level 1-4. At the top of each lab is the supervisor in each discipline, one for cytology, histology, grossing and administration. The usual problem in Histo labs is that people stay a long time and so block others from moving up, so even if a career ladder is in place advancement is difficult. Unless the department expands the opportunities to advance are limited in many labs. I histology we have had quite a lot of turnover the last 5 years because we had a 4 histotechs retire within a couple years so some advanced and some new techs came in. We also opened a couple new jobs. So we now have a fairly young lab with a lot of very good techs. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Steve via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 09, 2016 4:20 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pathology Ladder System Good morning everyone. I had a meeting recently with hospital administration where I was asked what leadership could do to retain and keep quality staff. Besides the obvious, pay increase's, I suggested a ladder type progression system for all lab staff, similar to what some hospitals offer nursing staff. My question to this group is, if any of you are using such a system would you mind sharing it with me? Thanks, Stephen Clark HT, BS, DESSO Pathology Dept. Supervisor Laboratory Safety Officer Grand Strand Medical Center 809 82nd Parkway Myrtle Beach, SC 29579 Desk: 843-692-1459 Lab: 843-692-1486 Fax: 843-449-6213 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 153, Issue 8 **************************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From Natalia_Zinchenko at hotmail.com Wed Aug 10 11:56:55 2016 From: Natalia_Zinchenko at hotmail.com (Natalia Zinchenko) Date: Wed, 10 Aug 2016 16:56:55 +0000 Subject: [Histonet] Histonet Digest, Vol 153, Issue 8 In-Reply-To: References: Message-ID: Hi, My name is Natalia. I have a question about tissue processor. If hypothetically (just hypothetically) I will not use charcoal filters and will change paraffin just sometimes, what is going to happen? I work in the research center and we don't have very many specimens. Thank you, Natalia ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, August 9, 2016 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 153, Issue 8 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Intraoperative tissue requisition or form (Vickroy, James) 2. Pan Cytokeratin (Paula Keene Pierce) 3. Decon Processor (Fawn Bomar) 4. Pathology Ladder System (Stephen.Clark1 at hcahealthcare.com) 5. Re: Pathology Ladder System (Duddey, Aimee) 6. **Histology Job Opportunities** (Taylor Rinaldi) 7. JOH journal collection (Connolly, Brett M) 8. Re: Pathology Ladder System (Anne Murvosh) 9. Re: Pathology Ladder System (Morken, Timothy) ---------------------------------------------------------------------- Message: 1 Date: Mon, 8 Aug 2016 20:23:21 +0000 From: "Vickroy, James" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Intraoperative tissue requisition or form Message-ID: <9B1A1501A800064397369BD8072E6BCA06594B45 at E2K10DB.springfieldclinic.com> Content-Type: text/plain; charset="us-ascii" We are a physician owned clinic and the number of frozen sections we do is small. Occasionally we have a few skin biopsies that are done in the ASC surgery department. I got a call from them today and they have a problem that they encounter every time they do a frozen section. When the surgeon takes the biopsy a nurse has to hold the specimen in surgery until the tissue order (requisition) is put into the computer. With staffing in ASC this may take fifteen minutes after the biopsy is removed and given to the nurse, especially if the surgeon is asking the nurse to do other things in the meantime. Of course when the specimen then is received by Histology it may take another 15-20 minutes before the surgeon gets a frozen section diagnosis. This morning the surgeon said this needs to get fixed. Of course it's easy for me to say to the ASC department that someone needs to put in the information immediately and not take fifteen minutes. I even asked why can't the order be put into the computer before the frozen is done. My other response to ASC was that we can do the frozen section but we can't assign a number not print barcoded slides and blocks until the order has been entered into the computer. I do recall that organizations with less robust IT systems than CoPath sometimes use a written Frozen section order form and report. Can anyone share with me a form they use currently that I could modify for our purposes? I know the basics but don't want to have to reinvent the wheel if someone already has a form that they use for this purpose. Of course once the nurses have had time to put the order into the computer histology would have to enter the case into the pathology system, assigning a surgical number. The pathologist would have to in turn take the diagnosis etc. off of the written sheet and enter the diagnosis and gross into the computer system. The written form would in turn have to be kept for documentation which can include scanning into the electronic health record and would include things like time surgeon is called, site, patient sticker with patient identifiers, clinical history, etc. I realize that this might seem like a step backward however given staffing issues I understand where the ASC nurse manager is coming from and if it helps the patient and surgeon I think we can live with the inconvenience. Your thoughts and examples of forms or frozen section requisitions? Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ------------------------------ Message: 2 Date: Mon, 8 Aug 2016 22:52:43 +0000 (UTC) From: Paula Keene Pierce To: Histonet Subject: [Histonet] Pan Cytokeratin Message-ID: <433712304.1847321.1470696763351.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Hello, would anyone be willing to donate a recently expired, about to be tossed, vial of pan cytokeratin for a research project? I will pay shipping. Thank you,?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com ------------------------------ Message: 3 Date: Tue, 9 Aug 2016 10:06:13 +0000 From: Fawn Bomar To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Decon Processor Message-ID: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA1FEF79 at EXCH-2K10.hrhs.com> Content-Type: text/plain; charset="iso-8859-1" Hi everyone, Our lab is getting ready to install a new processor. My question is, how do we go about decontaminating our old processor to dispose of it. The old processor is a Ventana Rennaissance. Thank you Fawn Bomar ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ------------------------------ Message: 4 Date: Tue, 9 Aug 2016 11:19:34 +0000 From: To: Subject: [Histonet] Pathology Ladder System Message-ID: Content-Type: text/plain; charset="us-ascii" Good morning everyone. I had a meeting recently with hospital administration where I was asked what leadership could do to retain and keep quality staff. Besides the obvious, pay increase's, I suggested a ladder type progression system for all lab staff, similar to what some hospitals offer nursing staff. My question to this group is, if any of you are using such a system would you mind sharing it with me? Thanks, Stephen Clark HT, BS, DESSO Pathology Dept. Supervisor Laboratory Safety Officer Grand Strand Medical Center 809 82nd Parkway Myrtle Beach, SC 29579 Desk: 843-692-1459 Lab: 843-692-1486 Fax: 843-449-6213 ------------------------------ Message: 5 Date: Tue, 9 Aug 2016 12:21:14 +0000 From: "Duddey, Aimee" To: "'Stephen.Clark1 at hcahealthcare.com'" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Pathology Ladder System Message-ID: <09FBA01CA9B6374A83C5C76E09E46188823EB11D at EXMAIL1-FHC.firsthealth.org> Content-Type: text/plain; charset="us-ascii" I have had the same discussion with lab administration recently. I would be interested in the same information. Aimee -----Original Message----- From: Steve via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 09, 2016 7:20 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pathology Ladder System Good morning everyone. I had a meeting recently with hospital administration where I was asked what leadership could do to retain and keep quality staff. Besides the obvious, pay increase's, I suggested a ladder type progression system for all lab staff, similar to what some hospitals offer nursing staff. My question to this group is, if any of you are using such a system would you mind sharing it with me? Thanks, Stephen Clark HT, BS, DESSO Pathology Dept. Supervisor Laboratory Safety Officer Grand Strand Medical Center 809 82nd Parkway Myrtle Beach, SC 29579 Desk: 843-692-1459 Lab: 843-692-1486 Fax: 843-449-6213 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 9 Aug 2016 08:39:52 -0400 From: "Taylor Rinaldi" To: Subject: [Histonet] **Histology Job Opportunities** Message-ID: <18bd01d1f23b$212ffb80$638ff280$@prometheushealthcare.com> Content-Type: text/plain; charset="us-ascii" Hi there! My name is Taylor Rinaldi, Recruiting Manager at Prometheus healthcare. My team and I specialize specifically in laboratory recruiting nationwide, working directly with lab managers from different hospitals and reference laboratories across the US . We are currently recruiting for multiple Histotechnologist and Histotechnician opportunities. All the opportunities are fulltime, and permanent. ASCP certification is preferred but not required for all. If you or any of your colleagues have been considering a new position in the Histology field, please don't hesitate to reach out to me directly for immediate referral and submittal to some of the top hospitals and labs nationwide. Current states: Chattanooga, Tennessee Sacramento, California New York, New York Atlanta, Georgia Dallas, Texas Thank you all in advance, Taylor Rinaldi Recruiting Manager Prometheus Healthcare Office 866-857-1434 Taylor at prometheushealthcare.com ------------------------------ Message: 7 Date: Tue, 9 Aug 2016 09:57:41 -0400 From: "Connolly, Brett M" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] JOH journal collection Message-ID: Content-Type: text/plain; charset="us-ascii" HI all, Is anyone interested in old issues of JOH? My collection spans 1997 - 2016. I am missing a few (~ 7). Let me know and I'll send you a list of what I have. Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 8 Date: Tue, 9 Aug 2016 13:56:31 +0000 From: Anne Murvosh To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Pathology Ladder System Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7B34236 at Exchange.Advancederm.net> Content-Type: text/plain; charset="us-ascii" If you don't already have it you may want to offer a shift differential for very early or late shifts and weekends. This makes it a little easier to hire for and keep people on the harder shifts. Anne -----Original Message----- From: Steve via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 09, 2016 4:20 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pathology Ladder System Good morning everyone. I had a meeting recently with hospital administration where I was asked what leadership could do to retain and keep quality staff. Besides the obvious, pay increase's, I suggested a ladder type progression system for all lab staff, similar to what some hospitals offer nursing staff. My question to this group is, if any of you are using such a system would you mind sharing it with me? Thanks, Stephen Clark HT, BS, DESSO Pathology Dept. Supervisor Laboratory Safety Officer Grand Strand Medical Center 809 82nd Parkway Myrtle Beach, SC 29579 Desk: 843-692-1459 Lab: 843-692-1486 Fax: 843-449-6213 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 9 Aug 2016 16:10:27 +0000 From: "Morken, Timothy" To: Histonet Subject: Re: [Histonet] Pathology Ladder System Message-ID: <761E2B5697F795489C8710BCC72141FF6FD7B06C at ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Our career ladder at UCSF HLT 1 thru 4 (Hospital Laboratory Technician) for non-histology positions. HLT-1 is entry level, could be out of high school. To move up requires more education. HLT-2 AA to BA/BA, HLT-3, -4, BA/BS used as accessioners, basic lab techs in grossing and cytology, running stainers, changing tissue processors in histology, etc. HLT-1,-2 are bench techs. HLT-3 is a Senior tech, HLT-4 is a Lead tech. HLT-3 and -4 can be trained for grossing. They can actually work their way thru to minor grossing with the right qualifications. If trained for histotechnology or more advanced grossing ie, PA-level) we promote them to the Histotechnologist series. HT 1 thru 4 (Histotechnologist). Traditional histology lab techs as well as on-the-job trained Pathologist's Assistants (HT-3,-4 only). HT-1,2 are the basic bench techs, HT-3, is Senior tech, HT-4 is Lead tech. Only HT-4 and Supervisor requires ASCP certification for histology work (not grossing work), but is "preferred" at the other levels. So far all our histotechs are ASCP certified HT or HTL. None of the OJT PA's are certified (though they could work for it if they desired). We do have an advantage of a large research population to draw techs from so all come to us with good educational background and have learned some histology in a research lab. These are all union positions and each classification (i.e HT-1 or HT-2) has 12 annual steps (controlled by the union, not administration) before maxing out in pay. At that point they reach a ceiling and can only move up by going to the next up classification. We usually require 5 years in one step before reclassifying unless the person is exceptional. As a manager I can only reclassify; I cannot move someone up a step as a reward for good work. This works out pretty well for advancement but we have a fairly large organization - 12 histotechs and 2 HLT in the histo lab; 4 HLT in cyto lab; 4 certifed PA's; 4 OJT PA's plus 5 HLT accessioners - moving up or to other positions within the department is possible. Several of our accessioners have moved up to Histotech positions, and one OJT PA went to PA school and came back to work for us. Another HLT accessioner is applying to PA school. We also now hire only certified PA's, and try to hire program-trained PA's. the competition for that is tough and for recent positions we have essentially opened it to any certified PA. Cyto uses the HLT series for lab work and has another Cytotechnologist series for cytotechs, same idea of level 1-4. At the top of each lab is the supervisor in each discipline, one for cytology, histology, grossing and administration. The usual problem in Histo labs is that people stay a long time and so block others from moving up, so even if a career ladder is in place advancement is difficult. Unless the department expands the opportunities to advance are limited in many labs. I histology we have had quite a lot of turnover the last 5 years because we had a 4 histotechs retire within a couple years so some advanced and some new techs came in. We also opened a couple new jobs. So we now have a fairly young lab with a lot of very good techs. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Steve via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 09, 2016 4:20 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pathology Ladder System Good morning everyone. I had a meeting recently with hospital administration where I was asked what leadership could do to retain and keep quality staff. Besides the obvious, pay increase's, I suggested a ladder type progression system for all lab staff, similar to what some hospitals offer nursing staff. My question to this group is, if any of you are using such a system would you mind sharing it with me? Thanks, Stephen Clark HT, BS, DESSO Pathology Dept. Supervisor Laboratory Safety Officer Grand Strand Medical Center 809 82nd Parkway Myrtle Beach, SC 29579 Desk: 843-692-1459 Lab: 843-692-1486 Fax: 843-449-6213 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 153, Issue 8 **************************************** From brett_connolly at merck.com Wed Aug 10 11:59:40 2016 From: brett_connolly at merck.com (Connolly, Brett M) Date: Wed, 10 Aug 2016 12:59:40 -0400 Subject: [Histonet] JOH journal collection --it's claimed Message-ID: All gone. Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Fawn.Bomar at HalifaxRegional.com Wed Aug 10 12:53:15 2016 From: Fawn.Bomar at HalifaxRegional.com (Fawn Bomar) Date: Wed, 10 Aug 2016 17:53:15 +0000 Subject: [Histonet] Disposal of Processor Message-ID: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA1FF09F@EXCH-2K10.hrhs.com> Does anyone know if you need to decontaminate a tissue processor in any special way to dispose of it? If the tissue has all been fixed before being in the processor and there were no CJD cases processed on it, is it considered biohazardous? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From barryrittman at gmail.com Wed Aug 10 12:57:22 2016 From: barryrittman at gmail.com (Barry Rittman) Date: Wed, 10 Aug 2016 12:57:22 -0500 Subject: [Histonet] Pathology Ladder System Message-ID: A well defined career ladder has often been a missing factor in salaries for histotechs. I believe that part of this is due to the change in the Med Lab Tech training System. Originally this system used to incoporate histology in the training. I believe in the early 70s the histology section was removed from the training and histopatholgy was on its own. This, in effect resulted in Med lab techs getting a degree and often being placed in charge of labs including histology/pathology labs without realy having much if any experience in these areas. I trained in England where histology was one of the six areas in med lab tech training in the entire country. Following this training it was possible to specialise in one of the fields such as histopathology. This provided a direct career path as everyone working in a lab all had the basic training and knew what to do to advance in their career. During my career, while I worked in histology (and this is still my first love), I was able to draw on my training in the other fields to help with my histopathology. I think that in effect the system of often having med lab techs in charge of histopatholgy have artifically kept salaries for histopathogists artificially low. I personally would prefer the med lab tech training (including histology) with a direct career path for all but am not holding my breath that this will occur Barry. From gentras at auburn.edu Thu Aug 11 10:49:22 2016 From: gentras at auburn.edu (Atoska Gentry) Date: Thu, 11 Aug 2016 15:49:22 +0000 Subject: [Histonet] specimen storage cabinets Message-ID: <4753f7d864004bf5a7a03483a50060ab@ex1.auburn.edu> Hello, I work for a research facility and we currently have an archive of several formalin fixed samples stored in 500ml glass specimen jars; one of our PI's recently asked me to conduct a search for storage cabinets specifically designed to accommodate these samples. Is anyone aware of the availability of such cabinets? The only thing my search has turned up thus far are the flammable metal cabinets. But due to limited storage space we have to stack these jars and the generic flammable cabinets are not stacked glass specimen jar suitable. All ideas & input are welcome. Thank you kindly, Atoska From renee at justmohs.com Thu Aug 11 11:05:21 2016 From: renee at justmohs.com (Renee Lightfeldt) Date: Thu, 11 Aug 2016 12:05:21 -0400 Subject: [Histonet] cm1100 leica Message-ID: <1437910906.1275.1470931521756.JavaMail.vpopmail@atl4oxapp03pod4> From mwerdler at gmail.com Thu Aug 11 11:26:52 2016 From: mwerdler at gmail.com (Mca Werdler) Date: Thu, 11 Aug 2016 09:26:52 -0700 Subject: [Histonet] Chicken eyes Message-ID: Hello everyone, I was wondering if you could help me out. I have some chicken eyes for research and i need a good fixation. My problem is that if i fixated the eyes in formalin for 48 hours, and i cut open the eyes, the eyes shrink a lot. Do i need a different fixative? or do i need longer fixation time? What are your experiences? I need a fixative, which is good foor IHC. THank you all Maarten From rjbuesa at yahoo.com Thu Aug 11 12:19:53 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 11 Aug 2016 17:19:53 +0000 (UTC) Subject: [Histonet] specimen storage cabinets In-Reply-To: <4753f7d864004bf5a7a03483a50060ab@ex1.auburn.edu> References: <4753f7d864004bf5a7a03483a50060ab@ex1.auburn.edu> Message-ID: <254487429.13110849.1470935993910.JavaMail.yahoo@mail.yahoo.com> Get regular metal cabinets used to store garage items.They are sold at any general store (such as HomeDepot or Walmart).Ren? On Thursday, August 11, 2016 12:05 PM, Atoska Gentry via Histonet wrote: Hello, I work for a research facility and we currently have an archive of several formalin fixed samples stored in 500ml glass specimen jars; one of our PI's recently asked me to conduct a search for storage cabinets specifically designed to accommodate these samples. Is anyone aware of the availability of? such cabinets? The only thing my search has turned up thus far are the flammable metal cabinets. But due to limited storage space we have to stack these jars and the generic flammable cabinets are not stacked glass specimen jar suitable. All ideas & input are welcome. Thank you kindly, Atoska _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jford at cytomx.com Thu Aug 11 12:22:43 2016 From: jford at cytomx.com (Judi Ford) Date: Thu, 11 Aug 2016 17:22:43 +0000 Subject: [Histonet] Freezing biopsies Message-ID: Hi everyone, Does anyone have experience working with human frozen biopsies? I have a question concerning freezing the biopsies. I am just doing general background research on this subject at the moment and have not worked with frozen human biopsies. If a biopsy was taken using a 14 gauge needle, in general, what is the length and diameter of a biopsy and what would the preferred method of freezing be (flash freezing in dry ice or freezing in liquid nitrogen)? What is the method for flash freezing in dry ice? What gauge of needles are most commonly used? Are most biopsies taken using a 16-18 gauge needle? Thanks for your help with this. I really appreciate it. Best regards, Judi Ford STATEMENT OF CONFIDENTIALITY: The information contained in this electronic message and any attachments to this message are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not an intended recipient, or a person responsible for delivering the e-mail to an intended recipient, please be advised that you have received this message in error and that any use, dissemination, forwarding, printing, or copying is strictly prohibited. Please notify the sender at CytomX Therapeutics, Inc., immediately and destroy all copies of this message and any attachments. CytomX Therapeutics, Inc. From mwerdler at gmail.com Thu Aug 11 12:40:31 2016 From: mwerdler at gmail.com (Mca Werdler) Date: Thu, 11 Aug 2016 10:40:31 -0700 Subject: [Histonet] Chicken eyes Message-ID: THank you all for the quick and wonderfull responses! I have some things to work out now and i will let you all know the result Thank you all so much Maarten From Timothy.Morken at ucsf.edu Thu Aug 11 13:00:01 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 11 Aug 2016 18:00:01 +0000 Subject: [Histonet] Freezing biopsies In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF6FD7BA63@ex07.net.ucsf.edu> Judi, what is the purpose of freezing it? For sectioning, biochemistry, histochemistry, or shipping it? It makes a difference. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Judi Ford via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, August 11, 2016 10:23 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Freezing biopsies Hi everyone, Does anyone have experience working with human frozen biopsies? I have a question concerning freezing the biopsies. I am just doing general background research on this subject at the moment and have not worked with frozen human biopsies. If a biopsy was taken using a 14 gauge needle, in general, what is the length and diameter of a biopsy and what would the preferred method of freezing be (flash freezing in dry ice or freezing in liquid nitrogen)? What is the method for flash freezing in dry ice? What gauge of needles are most commonly used? Are most biopsies taken using a 16-18 gauge needle? Thanks for your help with this. I really appreciate it. Best regards, Judi Ford STATEMENT OF CONFIDENTIALITY: The information contained in this electronic message and any attachments to this message are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not an intended recipient, or a person responsible for delivering the e-mail to an intended recipient, please be advised that you have received this message in error and that any use, dissemination, forwarding, printing, or copying is strictly prohibited. Please notify the sender at CytomX Therapeutics, Inc., immediately and destroy all copies of this message and any attachments. CytomX Therapeutics, Inc. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmilks at psquaredlabs.com Thu Aug 11 13:49:42 2016 From: cmilks at psquaredlabs.com (Clay Milks) Date: Thu, 11 Aug 2016 18:49:42 +0000 Subject: [Histonet] Pathology Partners, LLC HT/HTL Job opening Message-ID: We are growing! Pathology Partners, LLC in Little Rock, AR is adding an additional full time HT/HTL. Great pay and a great work environment. You must be a self-motivated, industrious worker. Health, Dental, Vision and 401k available. Please call Clay Milks at 501-687-9220 or email your resume to cmilks at psquaredlabs.com From SteveM at mcclainlab.com Thu Aug 11 16:42:06 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Thu, 11 Aug 2016 21:42:06 +0000 Subject: [Histonet] Histonet Digest, Vol 153, Issue 10 In-Reply-To: References: Message-ID: For once I almost refuse to answer because there is No good answer. This is absolutely ludicrous. Heck no is my first response but that probably doesn't pass muster. My state inspectors are probably wanting to have me fill out more firms forms for monthly bleaching. Or adding a bleach step to corrode the delicate inner bits. Perhaps the most cost effective method of disposal i am presently consider it may be least expensive to require all lab medical directors to put it in their will my wish is to be entombed with all 4 of my Kseries VIPs. They've been working longer than I have and refuse to die. I promise to retire those processors with me buried upright. Let me stand guard the rest of eternity to prevent the currently rampant spread of all diseases spread by contaminated. Shucks. I may have to be buried in Jamaica. I shipped a couple of those hazardous contaminated VIPs to Kingston. Heck I really do not know why we are pondering a non significant disease. Let someone identify a problem first before making a regulation or procedure. In my search for more data on the rampant waterski is on fire v71.0xA ICD10 problem, the processor contamination problem just snuck up on me!! I guess we need a decontamination room just to contain the outpouring of biohazardous waste every time we rotate solution or annual maintenance. Yep, let them cite me if my burial protocol fails the stink test. Steve A. McClain, MD From Timothy.Morken at ucsf.edu Thu Aug 11 17:07:45 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 11 Aug 2016 22:07:45 +0000 Subject: [Histonet] Disposal of Processor In-Reply-To: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA1FF09F@EXCH-2K10.hrhs.com> References: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA1FF09F@EXCH-2K10.hrhs.com> Message-ID: <761E2B5697F795489C8710BCC72141FF6FD7BBA9@ex07.net.ucsf.edu> Fawn, equipment we dispose of (ie, surplus for sale) we just have to clean it. For a tissue processor we would run a cleaning cycle, clean up the outside of crud and it is good to go. There is no infectious contamination issue. We signoff on a form that it has been decontaminated. It is similar to a form we sign if a piece of equipment has to be shipped to a vendor factory for repair (like an embedding center or cryostat). Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Fawn Bomar via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, August 10, 2016 10:53 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Disposal of Processor Does anyone know if you need to decontaminate a tissue processor in any special way to dispose of it? If the tissue has all been fixed before being in the processor and there were no CJD cases processed on it, is it considered biohazardous? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff at uropartners.com Fri Aug 12 08:23:29 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 12 Aug 2016 13:23:29 +0000 Subject: [Histonet] A Very Lab Blog to start your weekend. Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10F1E7D9@COLOEXCH01.uropartners.local> My appreciation to the lab world. http://www.chicagonow.com/downsize-maybe/2016/08/going-for-gold-in-a-different-competition/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From relia1 at earthlink.net Fri Aug 12 09:42:25 2016 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 12 Aug 2016 10:42:25 -0400 Subject: [Histonet] RELIA Hot Histology Job Alert 8-12-2016 TGIF!! Message-ID: <2e7b01d1f4a7$be24bed0$3a6e3c70$@earthlink.net> Hello Histonetters!! How are you doing today? Is it me or is this Summer FLYING by? I am sending this special alert out because I am ending the summer just like I started it with some of the best job opportunities out there. I am especially excited about these openings because they are with several of my best clients. Why do I refer to them as best clients? Well for a variety of reasons: Location, The rest of the team, The fantastic manager, Excellent benefits, Generous relocation And most importantly I like a client that has openings due to growth. Here is a list of the positions that I am most excited about: Histology Management/Supervisory Opportunities: Nashville, TN Histology Lab Manager Roswell, NM Assistant Supervisor Birmingham, AL Lead Histology Tech Fayetteville, AR Lead Histology Tech Histotechnicians/Histotechnologists Flagstaff, AZ Histo tech II - IHC/ISH Chattanooga, TN Grossing Histotech Birmingham, AL Lead Histotech Modesto, CA IHC Tech Fayetteville, AR Lead Histology Tech Louisville, KY Electron Microscopy Tech Ann Arbor, MI Histo tech (part time) If you or anyone you know is interested in more details, I can be reached toll free at 866-607-3542 or relia1 at earthlink.net. If you happen to know anyone who might be interested I am also recruiting for the following opportunities: MT/MLT - Various Locations in Virginia. - Sign on Bonus new grads welcome If you are starting to save up your Christmas money remember, $$$$ I pay a referral fee to you if I place someone that you refer to me. Thanks and have a great day!! Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From jpiche at wtbyhosp.org Fri Aug 12 10:04:19 2016 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Fri, 12 Aug 2016 15:04:19 +0000 Subject: [Histonet] Her2neu SP3 clone Message-ID: <631955447A364B45B9458D2905635110010778A251@WIN08-MBX-01.wtbyhosp.org> Hi Everyone! Happy Friday! I was wondering if anyone would mind providing the protocol for your SP3 IHC stain on the Dako instruments you have? We are setting up an Omnis and would like a starting point for the assay... Thanks, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From Nancy_Schmitt at pa-ucl.com Fri Aug 12 10:29:14 2016 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Fri, 12 Aug 2016 15:29:14 +0000 Subject: [Histonet] formalin/xylene monitor and grossing competency Message-ID: <27d1122025a94c93a6e484a42f56fb94@mercury.wad.pa-ucl.com> Good Morning - We are moving to a new facility and preparing to monitor - the question arose regarding whether it is acceptable to place monitors in the space instead of on each individual person. Ever hear of this? My other question is regarding proficiency/competency with HT's grossing. To prove competency do you cycle:25 derms, 25 GI, 15 endo, 15 cx, etc? follow up on small numbers yearly with competency packet? Thanks for your input- Nancy Schmitt MLT, HT(ASCP) Dubuque IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Nancy_Schmitt at pa-ucl.com Mon Aug 15 08:57:31 2016 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Mon, 15 Aug 2016 13:57:31 +0000 Subject: [Histonet] cryostat decontamination Message-ID: ANP.23410 states that cryostat is defrosted and wiped down at regular intervals. What if you have UV decontam? Is there a ruling on that? Thanks Nancy Schmitt MLT, HT(ASCP) Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From ADuddey at firsthealth.org Mon Aug 15 09:06:12 2016 From: ADuddey at firsthealth.org (Duddey, Aimee) Date: Mon, 15 Aug 2016 14:06:12 +0000 Subject: [Histonet] cryostat decontamination In-Reply-To: References: Message-ID: <09FBA01CA9B6374A83C5C76E09E46188823EF54D@EXMAIL1-FHC.firsthealth.org> Our last discussion with TJC to clarify their definition of "regular interval" was to follow the manufacturers guidelines. I recommend determining what manufacturer suggests with the use of the UV decon and write that in your policy that addresses this standard. If you are following their guidelines, you should be in compliance. Aimee -----Original Message----- From: Nancy Schmitt via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, August 15, 2016 9:58 AM To: ' Subject: [Histonet] cryostat decontamination ANP.23410 states that cryostat is defrosted and wiped down at regular intervals. What if you have UV decontam? Is there a ruling on that? Thanks Nancy Schmitt MLT, HT(ASCP) Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster at umn.edu Mon Aug 15 11:31:46 2016 From: cforster at umn.edu (Colleen Forster) Date: Mon, 15 Aug 2016 11:31:46 -0500 Subject: [Histonet] JOH journal collection In-Reply-To: References: Message-ID: Brett, Are you interested in sending all mof them as a complete (-7 issues) set? Colleen Forster U of MN On Tue, Aug 9, 2016 at 8:57 AM, Connolly, Brett M via Histonet < histonet at lists.utsouthwestern.edu> wrote: > HI all, > > Is anyone interested in old issues of JOH? My collection spans 1997 - > 2016. I am missing a few (~ 7). > > Let me know and I'll send you a list of what I have. > > Brett > > Brett M. Connolly, Ph.D. > Prin. Scientist, > Translational Biomarkers - Imaging > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly at merck.com > T- 215-652-2501 > F- 215-993-6803 > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, > New Jersey, USA 07033), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From c.tague at Pathologyarts.com Mon Aug 15 11:49:50 2016 From: c.tague at Pathologyarts.com (Curt) Date: Mon, 15 Aug 2016 16:49:50 +0000 Subject: [Histonet] automated special stains and control tissue Message-ID: <9C8F910F72893643B3C3793C3D67132B67D2C8D5@PATHOLOGYSERVER.pathologyarts.local> Re: automated special stainers, are you all running the patient tissue with a control on it, like IHC? we're wrestling with the idea of a control on each slide or a batch control... the pathologist wants a batch control, thinks the control may contaminate the patient tissue (PASF for example). My argument is that this process is different in that each slide is stained independently and should be treated as a separate stain, not like the old days running a bunch of slides in a Coplin jar... if there's a mechanical error and some reagent is dropped on a slide but the error didn't occur on the control slide then the control would still stain properly but the patient tissue would be negative... we will never know if there isn't a control. I think they should be treated like IHC... Anyone else have this issue? Thoughts? Curt From LAdams at CVPath.org Mon Aug 15 12:43:25 2016 From: LAdams at CVPath.org (Lila Adams) Date: Mon, 15 Aug 2016 17:43:25 +0000 Subject: [Histonet] CV Path Institute IHC-HT Job opportunity Message-ID: <7156d9c5aaae41bea120da0b77080601@Exchange01.CVPath.org> Good afternoon everyone, I thought I would post this job opportunity. CV Path Institute Job position: Histopathology/Immunohistochemistry technicians prepare pathology specimens for analysis by researchers and pathologists. Qualifications: Associates Degree; HT (ASCP) Histotechnician certification preferred and IHC qualification preferred. Familiarity with computers, Ventana IHC automation and common software would be a plus. General laboratory, Surgical and Autopsy Histopathology is a must. CVPath Institute Inc. is a not-for-profit-private pathology research laboratory based in Gaithersburg, MD focused on analysis of cardiac devices. Present staff includes about 44 employees, including three physicians, 2 scientists, multiple research fellows, five pathology assistants, 8 research associates and 5 research assistants. For further details apply to www. indeed.com Thank you, Lila R. Adams IHC Research Lab CVPath Institute, Inc 19 Firstfield Road Gaithersburg, MD 20878 301-208-3570 ext 138 This message is a PRIVATE communication. This message and all attachments are a private communication and may be confidential and/or legally privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the information contained in or attached to this message is strictly prohibited. Please notify the sender of the delivery error by replying to this message, and then delete it from your system. Thank you. From LRaff at uropartners.com Mon Aug 15 12:48:32 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 15 Aug 2016 17:48:32 +0000 Subject: [Histonet] Blog link broken in Dawns Post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10F2EEC1@COLOEXCH01.uropartners.local> For those of you trying to read the blog Dawn was referring to (and a lot of people seem to be), the URL was broken in her post. It should be: http://www.chicagonow.com/downsize-maybe/2016/08/going-for-gold-in-a-different-competition/ Dawn-how do labs responds to the problems you cite in your response? Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From cforster at umn.edu Mon Aug 15 12:52:49 2016 From: cforster at umn.edu (Colleen Forster) Date: Mon, 15 Aug 2016 12:52:49 -0500 Subject: [Histonet] CV Path Institute IHC-HT Job opportunity In-Reply-To: <7156d9c5aaae41bea120da0b77080601@Exchange01.CVPath.org> References: <7156d9c5aaae41bea120da0b77080601@Exchange01.CVPath.org> Message-ID: This sounds like a wonderful opportunity.....I wish I were in a position to go for it! C On Mon, Aug 15, 2016 at 12:43 PM, Lila Adams via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good afternoon everyone, > I thought I would post this job opportunity. > CV Path Institute Job position: Histopathology/Immunohistochemistry > technicians prepare pathology specimens for analysis by researchers and > pathologists. > Qualifications: Associates Degree; HT (ASCP) Histotechnician certification > preferred and IHC qualification preferred. Familiarity with computers, > Ventana IHC automation and common software would be a plus. General > laboratory, Surgical and Autopsy Histopathology is a must. > CVPath Institute Inc. is a not-for-profit-private pathology research > laboratory based in Gaithersburg, MD focused on analysis of cardiac > devices. Present staff includes about 44 employees, including three > physicians, 2 scientists, multiple research fellows, five pathology > assistants, 8 research associates and 5 research assistants. > For further details apply to www. indeed.com > Thank you, > Lila R. Adams > IHC Research Lab > CVPath Institute, Inc > 19 Firstfield Road > Gaithersburg, MD 20878 > 301-208-3570 ext 138 > > > > > This message is a PRIVATE communication. This message and all attachments > are a private communication and may be confidential and/or legally > privileged. If you are not the intended recipient, you are hereby notified > that any disclosure, copying, distribution or use of the information > contained in or attached to this message is strictly prohibited. Please > notify the sender of the delivery error by replying to this message, and > then delete it from your system. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From monica.aguilera at irbbarcelona.org Tue Aug 16 03:31:18 2016 From: monica.aguilera at irbbarcelona.org (Monica Aguilera) Date: Tue, 16 Aug 2016 10:31:18 +0200 Subject: [Histonet] oil red in WAT frozen samples Message-ID: Dears, I was wondering if some of you might have experience in the following: We have had a lot of problems cutting WAT tissue either directly embedded in OCT or pre-fixed with PFA or formalin (either cold or and them embedded with OCT. We solved this problem following Ryan Berry et al, 2014 paper protocol with gelatin. We get really nice cuts with that, but when performing the staining with oil red, we can not remove the gelatin from the cut and the oil red stays on the top of the tissue cut, it looks like there an oil-aqueous phase the does not let the oil red enter into the tissue.... Any suggest for removing the gelatin and for letting the oil red entering into the tissue will be more than welcome! Many thanks and kind regards, M?nica -- M?nica Aguilera Pujabet, DVM, PhD Histopathology Facility Institute for Research in Biomedicine - IRB Barcelona Baldiri Reixac, 10 E-08028 Barcelona - Spain Tel: +34 934033776 <%2B34%20934020546> monica.aguilera at irbbarcelona.org From j.benavides at eae.csic.es Tue Aug 16 05:08:01 2016 From: j.benavides at eae.csic.es (Julio Benavides) Date: Tue, 16 Aug 2016 12:08:01 +0200 Subject: [Histonet] Antibodies storage tips In-Reply-To: References: <1838985782.7452880.1470080384674.JavaMail.yahoo@mail.yahoo.com> Message-ID: <98140388-f747-452e-de28-438f0e2ec285@eae.csic.es> Hi all, In our lab we carry out quite a number of IHCs for research purposes. The number of mAbs and pAb stored in the frezeer is getting to the point that, when you need to do and IHC, you actually lose more time diving through the frezeer draws for the right vial than doing the IHC! is not a question than we are running our of freezer room, it is more to the point of having a proper and standarized way to keep the antibodies and than produce a proper registry of what we have and where to find it. I was wondering, in an attempt to avoid reinventing the wheel, if you could be as kind as to share your tips for storing and organizing the antibodies (aliquots, stock solutions, intermediate dilutions....) so chaos does not take over the lab! As always, thank you very much for your time and advice! Cheers Julio From mpence at grhs.net Tue Aug 16 08:08:59 2016 From: mpence at grhs.net (Mike Pence) Date: Tue, 16 Aug 2016 13:08:59 +0000 Subject: [Histonet] Placing formalin on specimens Message-ID: I know this might sound a bit crazy, but does anyone have a written policy and procedure for dispensing 10% formalin onto a surgical specimen? If you do, would you be willing to share? Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 mpence at grhs.net | www.greatrivermedical.org. www.Facebook.com/GreatRiverHealthSystems | www.Twitter/GreatRiverMed Information in this communication, including attachments, is confidential and intended only for the addressee(s). This communication may contain privileged, confidential, proprietary or trade secret information entitled to protection or exemption from disclosure under law. If you are not an intended recipient, please know that any use, distribution or copying of this communication, or any action taken based on the information in this communication, is unauthorized and may be unlawful. If you received this communication in error, please notify the sender and delete this communication from your device. From rjbuesa at yahoo.com Tue Aug 16 10:26:39 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 16 Aug 2016 15:26:39 +0000 (UTC) Subject: [Histonet] Placing formalin on specimens In-Reply-To: References: Message-ID: <229683134.14849875.1471361199583.JavaMail.yahoo@mail.yahoo.com> Usually you do not pour 10%NBF onto a specimen; you place the specimen onto a container/vial with the 10%NBF.Ren? On Tuesday, August 16, 2016 9:29 AM, Mike Pence via Histonet wrote: I know this might sound a bit crazy, but does anyone have a written policy and procedure for dispensing 10% formalin onto a surgical specimen? If you do, would you be willing to share? Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 mpence at grhs.net | www.greatrivermedical.org. www.Facebook.com/GreatRiverHealthSystems | www.Twitter/GreatRiverMed Information in this communication, including attachments, is confidential and intended only for the addressee(s). This communication may contain privileged, confidential, proprietary or trade secret information entitled to protection or exemption from disclosure under law. If you are not an intended recipient, please know that any use, distribution or copying of this communication, or any action taken based on the information in this communication, is unauthorized and may be unlawful. If you received this communication in error, please notify the sender and delete this communication from your device. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Tue Aug 16 10:30:36 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 16 Aug 2016 15:30:36 +0000 (UTC) Subject: [Histonet] Antibodies storage tips In-Reply-To: <98140388-f747-452e-de28-438f0e2ec285@eae.csic.es> References: <1838985782.7452880.1470080384674.JavaMail.yahoo@mail.yahoo.com> <98140388-f747-452e-de28-438f0e2ec285@eae.csic.es> Message-ID: <615054271.15045073.1471361436538.JavaMail.yahoo@mail.yahoo.com> I used to have several compartmented plastic alphabetized boxes with enough empty spaces to accommodate "new arrivals". If the spaces were used-up I just added a new box. Since they occupied several shelves, each shelf had the lettering identifying the boxes in each one.Ren? On Tuesday, August 16, 2016 6:29 AM, Julio Benavides via Histonet wrote: Hi all, In our lab we carry out quite a number of IHCs for research purposes. The number of mAbs and pAb stored in the frezeer is getting to the point that, when you need to do and IHC, you actually lose more time diving through the frezeer draws for the right vial than doing the IHC! is not a question than we are running our of freezer room, it is more to the point of having a proper and standarized way to keep the antibodies and than produce a proper registry of what we have and where to find it. I was wondering, in an attempt to avoid reinventing the wheel, if you could be as kind as to share your tips for storing and organizing the antibodies (aliquots, stock solutions, intermediate dilutions....) so chaos does not take over the lab! As always, thank you very much for your time and advice! Cheers Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Tue Aug 16 10:32:26 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 16 Aug 2016 15:32:26 +0000 (UTC) Subject: [Histonet] oil red in WAT frozen samples In-Reply-To: References: Message-ID: <796821013.14802964.1471361546259.JavaMail.yahoo@mail.yahoo.com> Apply gently heated water on the sections in a way that the gelatin is washed out.Ren? On Tuesday, August 16, 2016 4:48 AM, Monica Aguilera via Histonet wrote: Dears, I was wondering if some of you might have experience in the following: We have had a lot of problems cutting WAT tissue either directly embedded in OCT or pre-fixed with PFA or formalin (either cold or and them embedded with OCT. We solved this problem following Ryan Berry et al, 2014 paper protocol with gelatin. We get really nice cuts with that, but when performing the staining with oil red, we can not remove the gelatin from the cut and the oil red stays on the top of the tissue cut, it looks like there an oil-aqueous phase the does not let the oil red enter into the tissue.... Any suggest for removing the gelatin and for letting the oil red entering into the tissue will be more than welcome! Many thanks and kind regards, M?nica -- M?nica Aguilera Pujabet, DVM, PhD Histopathology Facility Institute for Research in Biomedicine - IRB Barcelona Baldiri Reixac, 10 E-08028 Barcelona - Spain Tel:? +34 934033776 <%2B34%20934020546> monica.aguilera at irbbarcelona.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston at nationwidechildrens.org Tue Aug 16 10:44:43 2016 From: Ronald.Houston at nationwidechildrens.org (Houston, Ronald) Date: Tue, 16 Aug 2016 15:44:43 +0000 Subject: [Histonet] Caspase 3 and Tunel Assay Message-ID: Is anyone working on this just now? And more specifically on the pig species? Any pointers would be appreciated Thanks Ronnie Houston, MS HT(ASCP)QIHC FIBMS Anatomic Pathology Manager Laboratory Services 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston at nationwidechildrens.org www.NationwideChildrens.org "Without continual growth and progress, such words as improvement, achievement, and success have no meaning." ~ Ben Franklin From liz at premierlab.com Tue Aug 16 11:01:30 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Tue, 16 Aug 2016 10:01:30 -0600 Subject: [Histonet] Caspase 3 and Tunel Assay In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02BED535B63B@SBS2K8.premierlab.local> Ronnie We have these protocols in place for porcine tissue. We use the roche kit for tunel 11684809910 and for CC3 we use the cell signaling antibody 9664. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Houston, Ronald via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 16, 2016 9:45 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Caspase 3 and Tunel Assay Is anyone working on this just now? And more specifically on the pig species? Any pointers would be appreciated Thanks Ronnie Houston, MS HT(ASCP)QIHC FIBMS Anatomic Pathology Manager Laboratory Services 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston at nationwidechildrens.org www.NationwideChildrens.org "Without continual growth and progress, such words as improvement, achievement, and success have no meaning." ~ Ben Franklin _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz at premierlab.com Tue Aug 16 11:05:51 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Tue, 16 Aug 2016 10:05:51 -0600 Subject: [Histonet] Antibodies storage tips In-Reply-To: <615054271.15045073.1471361436538.JavaMail.yahoo@mail.yahoo.com> References: <1838985782.7452880.1470080384674.JavaMail.yahoo@mail.yahoo.com> <98140388-f747-452e-de28-438f0e2ec285@eae.csic.es> <615054271.15045073.1471361436538.JavaMail.yahoo@mail.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE02BED535B63C@SBS2K8.premierlab.local> We use freezer boxes and then record what is in the freezer boxes on a worksheet that is kept in an excel file. The excel file has all of the boxes in it. Here is an example of one of the pages BOX 6 - Full name vendor catalog # lot # date alqt ul/aliquot notes AGE Biorbyt orb10064 A1268 5/7/14 25ul CSPG (CS-56) Sigma C8035 032M4824 5/19/14 20ul retest 2/2017 CSPG (CS-56) Sigma C8035 026M4834V need aliq. (2x200ul); retest 10/2020 Alk Phos-ALPL abcam ab17973 GR157978-1 8/15/14 10ul Osterix/Sp7 abcam ab22552 GR138927-1 5/30/14 10ul retest 5/28/19 Osterix/Sp7 abcam ab22552 GR176974-2 3/5/15 10ul Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 16, 2016 9:31 AM To: Julio Benavides; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Antibodies storage tips I used to have several compartmented plastic alphabetized boxes with enough empty spaces to accommodate "new arrivals". If the spaces were used-up I just added a new box. Since they occupied several shelves, each shelf had the lettering identifying the boxes in each one.Ren? On Tuesday, August 16, 2016 6:29 AM, Julio Benavides via Histonet > wrote: Hi all, In our lab we carry out quite a number of IHCs for research purposes. The number of mAbs and pAb stored in the frezeer is getting to the point that, when you need to do and IHC, you actually lose more time diving through the frezeer draws for the right vial than doing the IHC! is not a question than we are running our of freezer room, it is more to the point of having a proper and standarized way to keep the antibodies and than produce a proper registry of what we have and where to find it. I was wondering, in an attempt to avoid reinventing the wheel, if you could be as kind as to share your tips for storing and organizing the antibodies (aliquots, stock solutions, intermediate dilutions....) so chaos does not take over the lab! As always, thank you very much for your time and advice! Cheers Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KSimeone at leavittmgt.com Tue Aug 16 12:28:46 2016 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Tue, 16 Aug 2016 17:28:46 +0000 Subject: [Histonet] FT OVERNIGHT HT POSTITION Delray Beach, FL In-Reply-To: <43944B1DBAAC2846B7B9D626B5F1233CC91950B2@vm-email.leavittmgt.com> References: <43944B1DBAAC2846B7B9D626B5F1233CC9194C6C@vm-email.leavittmgt.com>, <43944B1DBAAC2846B7B9D626B5F1233CC91950B2@vm-email.leavittmgt.com> Message-ID: <43944B1DBAAC2846B7B9D626B5F1233CC91959E5@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed HISTOTECHNOLOGIST here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Please fill out employment application HERE http://www.indeed.com/m/viewjob?jk=266fccd1fa688bb6&from=serp ^^you MUST follow this application link to apply! No exceptions. *full time position Mon-Fri OR Sun-Thurs 10p-6:30AM *MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo most of your shift) *MUST have at LEAST FIVE (5) years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE! *VERY proficient in embedding and microtomy *WILL MOSTLY BE EMBEDDING EXCISION BLOCKS, please know DERMS *must be self motivated, reliable and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) *some IHC experience preferred but not necessary Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor Delray Beach Technical Laboratory ADCS Clinics, LLC Advanced Dermatology and Cosmetic Surgery Ameriderm www.advancedderm.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From Richard.Cartun at hhchealth.org Tue Aug 16 12:48:06 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 16 Aug 2016 17:48:06 +0000 Subject: [Histonet] ATM IHC Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E95379E2A@HHCEXCHMB03.hhcsystem.org> Anyone doing IHC testing for "Ataxia-telangiectasia-mutated protein" expression on FFPE tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From mills at 3scan.com Tue Aug 16 13:05:55 2016 From: mills at 3scan.com (Caroline Miller) Date: Tue, 16 Aug 2016 11:05:55 -0700 Subject: [Histonet] ATM IHC In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E95379E2A@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E95379E2A@HHCEXCHMB03.hhcsystem.org> Message-ID: I have done this in the research world, but I don't have any experience with clinical analysis of this protein. I will tell you that cell signalling have the best antibodies in this space, IMHO: https://www.cellsignal.com/browse/?Ntk=Products&N=4294956287&Ntt=atm+ A company that you can actually follow their protocols and it works! (no commercial interest) yours, mills On Tue, Aug 16, 2016 at 10:48 AM, Cartun, Richard via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Anyone doing IHC testing for "Ataxia-telangiectasia-mutated protein" > expression on FFPE tissue? Thank you. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & > Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From eca9 at georgetown.edu Wed Aug 17 08:14:42 2016 From: eca9 at georgetown.edu (Eva Permaul) Date: Wed, 17 Aug 2016 09:14:42 -0400 Subject: [Histonet] Gallocyanine Message-ID: Good morning out there, We just had a request for Gallocyanine staining. Does anyone do this? Can you share your protocol? Where do you buy your reagents? Thanks in advance for any help in the right direction, Eva Permaul Georgetown University From corey at rankinbiomed.com Wed Aug 17 13:15:29 2016 From: corey at rankinbiomed.com (Corey Hubbard) Date: Wed, 17 Aug 2016 14:15:29 -0400 Subject: [Histonet] update Message-ID: Hi Eva, I wanted to see how things are going for you. Please let me know if you come across any equipment needs. We specialize in histology and pathology equipment and service. We are also always looking to purchase equipment - broken, used, collecting dust. etc. Do you have a vendor you use currently for equipment? *Corey Hubbard * Sales Account Manager / Marketing Admin 248.215.5390 ext 0022 *RANKIN* 14515 Mackey Road Holly, MI 48442 www.rankinbiomed.com facebook.com/rankinbiomed twitter.com/rankinbiomed.com *John 8:12* Jesus spoke to them, saying "I am the light of the world; he who follows me will not walk in darkness, but will have the light of life. From rsrichmond at gmail.com Wed Aug 17 18:58:38 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Wed, 17 Aug 2016 19:58:38 -0400 Subject: [Histonet] Gallocyanine Message-ID: Eva Permaul at Georgetown University asks: >>We just had a request for Gallocyanine staining. Does anyone do this? Can you share your protocol? Where do you buy your reagents?<< Gallocyanin (correct spelling - C.I. 51030) is a blue oxazine dye somewhat similar to celestin blue B. It's been used as a stain for Nissl substance. Somebody at the AFIP was touting it as a hematoxylin substitute during the Great Hematoxylin Shortage of around 1972. I think it was used as a chrome alum lake for a nuclear stain. I've never seen it. You can get the dry dye from Sigma-Aldrich. John Kiernan's book and Bancroft & Stevens 4th ed both have formulas. But what does your client want to do with it? Bob Richmond Samurai Pathologist Maryville TN From liz at premierlab.com Thu Aug 18 09:03:45 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Thu, 18 Aug 2016 08:03:45 -0600 Subject: [Histonet] Gallocyanine In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02BED535B69A@SBS2K8.premierlab.local> We have run this stain before, purchased the reagent from Sigma, it's pretty easy to do there is a procedure in Bancroft and Gamble. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, August 17, 2016 5:59 PM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Gallocyanine Eva Permaul at Georgetown University asks: >>We just had a request for Gallocyanine staining. Does anyone do this? >>Can you share your protocol? Where do you buy your reagents?<< Gallocyanin (correct spelling - C.I. 51030) is a blue oxazine dye somewhat similar to celestin blue B. It's been used as a stain for Nissl substance. Somebody at the AFIP was touting it as a hematoxylin substitute during the Great Hematoxylin Shortage of around 1972. I think it was used as a chrome alum lake for a nuclear stain. I've never seen it. You can get the dry dye from Sigma-Aldrich. John Kiernan's book and Bancroft & Stevens 4th ed both have formulas. But what does your client want to do with it? Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From janzaldi at medequipsource.com Thu Aug 18 12:23:34 2016 From: janzaldi at medequipsource.com (Jim Anzaldi) Date: Thu, 18 Aug 2016 13:23:34 -0400 Subject: [Histonet] Histonet Digest, Vol 153, Issue 15 In-Reply-To: References: Message-ID: <2C7597ABF5467247BE159D763E27F839016714B99787@PROMETHEUS.medequip.local> Vendor is soliciting sales item #1 -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, August 18, 2016 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 153, Issue 15 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. update (Corey Hubbard) 2. Re: Gallocyanine (Bob Richmond) 3. Re: Gallocyanine (Elizabeth Chlipala) ---------------------------------------------------------------------- Message: 1 Date: Wed, 17 Aug 2016 14:15:29 -0400 From: Corey Hubbard To: histonet at lists.utsouthwestern.edu Subject: [Histonet] update Message-ID: Content-Type: text/plain; charset=UTF-8 Hi Eva, I wanted to see how things are going for you. Please let me know if you come across any equipment needs. We specialize in histology and pathology equipment and service. We are also always looking to purchase equipment - broken, used, collecting dust. etc. Do you have a vendor you use currently for equipment? *Corey Hubbard * Sales Account Manager / Marketing Admin 248.215.5390 ext 0022 *RANKIN* 14515 Mackey Road Holly, MI 48442 www.rankinbiomed.com facebook.com/rankinbiomed twitter.com/rankinbiomed.com *John 8:12* Jesus spoke to them, saying "I am the light of the world; he who follows me will not walk in darkness, but will have the light of life. ------------------------------ Message: 2 Date: Wed, 17 Aug 2016 19:58:38 -0400 From: Bob Richmond To: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Gallocyanine Message-ID: Content-Type: text/plain; charset=UTF-8 Eva Permaul at Georgetown University asks: >>We just had a request for Gallocyanine staining. Does anyone do this? >>Can you share your protocol? Where do you buy your reagents?<< Gallocyanin (correct spelling - C.I. 51030) is a blue oxazine dye somewhat similar to celestin blue B. It's been used as a stain for Nissl substance. Somebody at the AFIP was touting it as a hematoxylin substitute during the Great Hematoxylin Shortage of around 1972. I think it was used as a chrome alum lake for a nuclear stain. I've never seen it. You can get the dry dye from Sigma-Aldrich. John Kiernan's book and Bancroft & Stevens 4th ed both have formulas. But what does your client want to do with it? Bob Richmond Samurai Pathologist Maryville TN ------------------------------ Message: 3 Date: Thu, 18 Aug 2016 08:03:45 -0600 From: Elizabeth Chlipala To: Bob Richmond , "'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)" Subject: Re: [Histonet] Gallocyanine Message-ID: <14E2C6176416974295479C64A11CB9AE02BED535B69A at SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" We have run this stain before, purchased the reagent from Sigma, it's pretty easy to do there is a procedure in Bancroft and Gamble. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, August 17, 2016 5:59 PM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Gallocyanine Eva Permaul at Georgetown University asks: >>We just had a request for Gallocyanine staining. Does anyone do this? >>Can you share your protocol? Where do you buy your reagents?<< Gallocyanin (correct spelling - C.I. 51030) is a blue oxazine dye somewhat similar to celestin blue B. It's been used as a stain for Nissl substance. Somebody at the AFIP was touting it as a hematoxylin substitute during the Great Hematoxylin Shortage of around 1972. I think it was used as a chrome alum lake for a nuclear stain. I've never seen it. You can get the dry dye from Sigma-Aldrich. John Kiernan's book and Bancroft & Stevens 4th ed both have formulas. But what does your client want to do with it? Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 153, Issue 15 ***************************************** From rsrichmond at gmail.com Thu Aug 18 19:01:51 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 18 Aug 2016 20:01:51 -0400 Subject: [Histonet] Gallocyanin Message-ID: Liz Chlipala at Premier Laboratory in Boulder CO writes: >>We have run this stain [gallocyanin] before, purchased the reagent from Sigma, it's pretty easy to do there is a procedure in Bancroft and Gamble.<< What stain? What mordant (chrome, aluminum, none)? To stain what? I think the person originally posting needs to extract this information from the person who wants the stain done. Bob Richmond Samurai Pathologist Maryville TN From liz at premierlab.com Fri Aug 19 09:24:06 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Fri, 19 Aug 2016 08:24:06 -0600 Subject: [Histonet] Gallocyanin - protocol Message-ID: <14E2C6176416974295479C64A11CB9AE02BED535B6BD@SBS2K8.premierlab.local> We used the stain to stain nissl substance in mouse lumbar spine. Purchased the dye from sigma - below is from the SDS Sheet Product name : Gallocyanine Product Number : 124508 Brand : Sigma-Aldrich CAS-No. : 1562-85-2 The procedure in Bancroft-Gamble uses Chrom Alum - which is chromium potassium sulfate as the mordant Chrom Alum - 5 gm dH20 - 100 ml Gallocyanine - 50 mg The chrome alum is dissolved in the distilled water, the gallocyanine is added and the solution slowly heated until it boils. Boil for 5 minutes. Cool to room temp adjust the volume back to 100 ml. Filter before use The stain is easy - place in staining solution for 18-48 hours, rinse in water, dehydrate, clear and mount. B&G states results are blue but If I remember correctly it turned out to be a bluish grey in color. I tried to see if I had any images but I could not find any, we did this back in 2006 so it was some time ago. If you search the histonet archives there is an older post of mine looking for some help on this stain, there is a nice response back from Dr. Kiernan explaining a bit more about the dye and staining. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, August 18, 2016 6:02 PM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Gallocyanin Liz Chlipala at Premier Laboratory in Boulder CO writes: >>We have run this stain [gallocyanin] before, purchased the reagent >>from Sigma, it's pretty easy to do there is a procedure in Bancroft and Gamble.<< What stain? What mordant (chrome, aluminum, none)? To stain what? I think the person originally posting needs to extract this information from the person who wants the stain done. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Fri Aug 19 09:38:59 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 19 Aug 2016 10:38:59 -0400 Subject: [Histonet] Gallocyanine Message-ID: Here's John Kiernan's extremely informative review of gallocyanine (that's how he says it's to be spelled) from the archives - I can't find a date on it. Really tells you everything you need to know to do Nissl staining with it. http://www.histosearch.com/histonet/May06/Re.Histonetgallocyaninest.html Remember to check on hazardous waste issues for chromium. Bob Richmond Samurai Pathologist Maryville TN From corey at rankinbiomed.com Fri Aug 19 12:13:09 2016 From: corey at rankinbiomed.com (Corey Hubbard) Date: Fri, 19 Aug 2016 13:13:09 -0400 Subject: [Histonet] Histonet Digest, Vol 153, Issue 16 In-Reply-To: References: Message-ID: Hi Jim/Histonet world, This was a mistake and was not supposed to be posted. Histonet has already been emailed. Thank you! :) Message: 1 Date: Thu, 18 Aug 2016 13:23:34 -0400 From: Jim Anzaldi To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Histonet Digest, Vol 153, Issue 15 Message-ID: <2C7597ABF5467247BE159D763E27F839016714B99787 at PROMETHEUS. medequip.local> Content-Type: text/plain; charset="us-ascii" Vendor is soliciting sales item #1 *Corey Hubbard * Sales Account Manager / Marketing Admin 248.215.5390 ext 0022 *RANKIN* 14515 Mackey Road Holly, MI 48442 www.rankinbiomed.com facebook.com/rankinbiomed twitter.com/rankinbiomed.com *John 8:12* Jesus spoke to them, saying "I am the light of the world; he who follows me will not walk in darkness, but will have the light of life. On Fri, Aug 19, 2016 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Histonet Digest, Vol 153, Issue 15 (Jim Anzaldi) > 2. Re: Gallocyanin (Bob Richmond) > 3. Re: Gallocyanin - protocol (Elizabeth Chlipala) > 4. Re: Gallocyanine (Bob Richmond) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 18 Aug 2016 13:23:34 -0400 > From: Jim Anzaldi > To: "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Histonet Digest, Vol 153, Issue 15 > Message-ID: > <2C7597ABF5467247BE159D763E27F839016714B99787 at PROMETHEUS. > medequip.local> > > Content-Type: text/plain; charset="us-ascii" > > Vendor is soliciting sales item #1 > > -----Original Message----- > From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request@ > lists.utsouthwestern.edu] > Sent: Thursday, August 18, 2016 1:00 PM > To: histonet at lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 153, Issue 15 > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific than > "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. update (Corey Hubbard) > 2. Re: Gallocyanine (Bob Richmond) > 3. Re: Gallocyanine (Elizabeth Chlipala) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 17 Aug 2016 14:15:29 -0400 > From: Corey Hubbard > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] update > Message-ID: > gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Hi Eva, > > I wanted to see how things are going for you. Please let me know if you > come across any equipment needs. We specialize in histology and pathology > equipment and service. > > We are also always looking to purchase equipment - broken, used, > collecting dust. etc. > > Do you have a vendor you use currently for equipment? > > > > *Corey Hubbard * > Sales Account Manager / Marketing Admin > 248.215.5390 ext 0022 > > *RANKIN* > 14515 Mackey Road > Holly, MI 48442 > www.rankinbiomed.com > facebook.com/rankinbiomed > twitter.com/rankinbiomed.com > > *John 8:12* Jesus spoke to them, saying "I am the light of the world; he > who follows me will not walk in darkness, but will have the light of life. > > > ------------------------------ > > Message: 2 > Date: Wed, 17 Aug 2016 19:58:38 -0400 > From: Bob Richmond > To: "Histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Gallocyanine > Message-ID: > gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Eva Permaul at Georgetown University asks: > > >>We just had a request for Gallocyanine staining. Does anyone do this? > >>Can > you share your protocol? Where do you buy your reagents?<< > > Gallocyanin (correct spelling - C.I. 51030) is a blue oxazine dye somewhat > similar to celestin blue B. It's been used as a stain for Nissl substance. > Somebody at the AFIP was touting it as a hematoxylin substitute during the > Great Hematoxylin Shortage of around 1972. I think it was used as a chrome > alum lake for a nuclear stain. I've never seen it. You can get the dry dye > from Sigma-Aldrich. John Kiernan's book and Bancroft & Stevens 4th ed both > have formulas. But what does your client want to do with it? > > Bob Richmond > Samurai Pathologist > Maryville TN > > > ------------------------------ > > Message: 3 > Date: Thu, 18 Aug 2016 08:03:45 -0600 > From: Elizabeth Chlipala > To: Bob Richmond , > "'histonet at lists.utsouthwestern.edu' > (histonet at lists.utsouthwestern.edu)" > > Subject: Re: [Histonet] Gallocyanine > Message-ID: > <14E2C6176416974295479C64A11CB9AE02BED535B69A at SBS2K8. > premierlab.local> > Content-Type: text/plain; charset="us-ascii" > > We have run this stain before, purchased the reagent from Sigma, it's > pretty easy to do there is a procedure in Bancroft and Gamble. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box > 18592 Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz at premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, August 17, 2016 5:59 PM > To: Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Gallocyanine > > Eva Permaul at Georgetown University asks: > > >>We just had a request for Gallocyanine staining. Does anyone do this? > >>Can > you share your protocol? Where do you buy your reagents?<< > > Gallocyanin (correct spelling - C.I. 51030) is a blue oxazine dye somewhat > similar to celestin blue B. It's been used as a stain for Nissl substance. > Somebody at the AFIP was touting it as a hematoxylin substitute during the > Great Hematoxylin Shortage of around 1972. I think it was used as a chrome > alum lake for a nuclear stain. I've never seen it. You can get the dry dye > from Sigma-Aldrich. John Kiernan's book and Bancroft & Stevens 4th ed both > have formulas. But what does your client want to do with it? > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 153, Issue 15 > ***************************************** > > > > > ------------------------------ > > Message: 2 > Date: Thu, 18 Aug 2016 20:01:51 -0400 > From: Bob Richmond > To: "Histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Gallocyanin > Message-ID: > gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Liz Chlipala at Premier Laboratory in Boulder CO writes: > > >>We have run this stain [gallocyanin] before, purchased the reagent from > Sigma, it's pretty easy to do there is a procedure in Bancroft and > Gamble.<< > > What stain? What mordant (chrome, aluminum, none)? To stain what? > > I think the person originally posting needs to extract this information > from the person who wants the stain done. > > Bob Richmond > Samurai Pathologist > Maryville TN > > > ------------------------------ > > Message: 3 > Date: Fri, 19 Aug 2016 08:24:06 -0600 > From: Elizabeth Chlipala > To: Bob Richmond , > "'histonet at lists.utsouthwestern.edu' > (histonet at lists.utsouthwestern.edu)" > , "eca9 at georgetown.edu" > > Subject: Re: [Histonet] Gallocyanin - protocol > Message-ID: > <14E2C6176416974295479C64A11CB9AE02BED535B6BD at SBS2K8. > premierlab.local> > Content-Type: text/plain; charset="us-ascii" > > We used the stain to stain nissl substance in mouse lumbar spine. > > Purchased the dye from sigma - below is from the SDS Sheet > > Product name : Gallocyanine > Product Number : 124508 > Brand : Sigma-Aldrich > CAS-No. : 1562-85-2 > > The procedure in Bancroft-Gamble uses Chrom Alum - which is chromium > potassium sulfate as the mordant > > Chrom Alum - 5 gm > dH20 - 100 ml > Gallocyanine - 50 mg > > The chrome alum is dissolved in the distilled water, the gallocyanine is > added and the solution slowly heated until it boils. Boil for 5 minutes. > Cool to room temp adjust the volume back to 100 ml. Filter before use > > The stain is easy - place in staining solution for 18-48 hours, rinse in > water, dehydrate, clear and mount. B&G states results are blue but If I > remember correctly it turned out to be a bluish grey in color. I tried to > see if I had any images but I could not find any, we did this back in 2006 > so it was some time ago. > > If you search the histonet archives there is an older post of mine looking > for some help on this stain, there is a nice response back from Dr. Kiernan > explaining a bit more about the dye and staining. > > Liz > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz at premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > -----Original Message----- > From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, August 18, 2016 6:02 PM > To: Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Gallocyanin > > Liz Chlipala at Premier Laboratory in Boulder CO writes: > > >>We have run this stain [gallocyanin] before, purchased the reagent > >>from > Sigma, it's pretty easy to do there is a procedure in Bancroft and > Gamble.<< > > What stain? What mordant (chrome, aluminum, none)? To stain what? > > I think the person originally posting needs to extract this information > from the person who wants the stain done. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Fri, 19 Aug 2016 10:38:59 -0400 > From: Bob Richmond > To: Elizabeth Chlipala > Cc: "'histonet at lists.utsouthwestern.edu' > (histonet at lists.utsouthwestern.edu)" > , "eca9 at georgetown.edu" > > Subject: Re: [Histonet] Gallocyanine > Message-ID: > gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Here's John Kiernan's extremely informative review of gallocyanine (that's > how he says it's to be spelled) from the archives - I can't find a date on > it. Really tells you everything you need to know to do Nissl staining with > it. > > http://www.histosearch.com/histonet/May06/Re.Histonetgallocyaninest.html > > Remember to check on hazardous waste issues for chromium. > > Bob Richmond > Samurai Pathologist > Maryville TN > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 153, Issue 16 > ***************************************** > From patpxs at gmail.com Fri Aug 19 12:32:58 2016 From: patpxs at gmail.com (P Sicurello) Date: Fri, 19 Aug 2016 10:32:58 -0700 Subject: [Histonet] UCSD Hiring a Histology Supervisor Message-ID: Good Morning Netters,UC San Diego Health is opening a new hospital and is actively looking for a supervisor for the Histology, IHC and EM labs. You will be supervising a staff of 12 (soon to be 13). This is the job code: #JMC83529. This is the job title: Histotechnologist Supervisor Go to jobs.ucsd.edu to see the details and apply if interested. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From Linda.Margraf at cookchildrens.org Fri Aug 19 13:59:39 2016 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Fri, 19 Aug 2016 18:59:39 +0000 Subject: [Histonet] Corey's apology Message-ID: <95fb20a0a5974af0a85324aff958da1b@MBX10.CCHCS.LDAP> Hi Histonetters, Corey Hubbard at Rankin sent me a message earlier today to apologize for accidently sending a message he intended to go to one person, out to the entire list. He did not intend to "advertise" and wanted to see if I could retract the message. Unfortunately, I cannot retract messages that go to the Histonet server and it has already gone out to the list. Anyway, the rules still apply about vendors. They are always welcome and encouraged to post to the list regarding subscribers questions and concerns but we cannot allow the forum to be used for advertising as it is run by the University of Texas and must remain an unbiased platform. Thanks everyone for their continued participation on Histonet, by the way. We still have>3300 subscribers which is highly unusual for a listserv that has been in operation for nearly 20 years! Linda M Histonet administrator From blayjorge at gmail.com Fri Aug 19 14:23:41 2016 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Fri, 19 Aug 2016 15:23:41 -0400 Subject: [Histonet] Query on authorship Message-ID: Query on authorship Dear Colleagues: I am writing a small paper resulting from research done with two undergraduates many years ago (and, later on, involving several other colleagues using cutting-edge technology). As the results became obvious, both of the students agreed (orally, in person) with me that we should get the research published. As far as I remember, there was no email or letter documenting that and, there was no manuscript, only the data and the methods we were using. The problem: I have located one of the former students (now a researcher at a major research institution), who is excited about getting the research published, but not the second student. Question: How to handle the contribution (including authorship) of the other person? Here are some options I see. a. *Omit the name of the person that has not been located* and indicate that another person was involved in the data collection but we were hot able to locate him/her to get his/her approval to use his/her name as an author. Under these circumstances, would it be OK to name the person in the Acknowledgments? Lately, I am asking permission to do that because sometimes some people prefer to remain anonymous. b. *Include the name of the person I cannot locate as an author*, an act of fairness and good faith on my part. If the person does not like the idea (and the paper is published) retract the name of the person in an erratum, later on, and assume responsibility for my error. A kind colleague did that to me once and, subsequently, it has resulted a long standing collaboration (and co-authorship in many papers, with my knowledge) :) c. *Nor use the data garnered by the person I cannot locate*. Although I am pretty sure I am authorized by the institution to use the data, as a general personal; preference, I like to ask permission. If you have something constructive to comment, kindly direct your comments to me, blayjorge at gmail.com , Apologies for potential cross-posting. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From rjbuesa at yahoo.com Sat Aug 20 12:18:01 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Sat, 20 Aug 2016 17:18:01 +0000 (UTC) Subject: [Histonet] Query on authorship In-Reply-To: References: Message-ID: <585799190.314651.1471713481783@mail.yahoo.com> Jorge:The first thing is to be absolutely sure the data is worth publishing and that the results have scientific relevance.If this is the case and both you and the other contributor agree I think the data should be published.In no way you should eliminate the data obtained by the other individual you have been unable to locate because that will weaken the results. If what the two students did was obtaining data it is fair to add the name of the one accepting to be considered as co-author, but ONLY if s/he takes part of data processing and the conclusions.As to the other student you have been unable to locate I do NOT think you should make him/her as co-author because just obtaining raw data does not amount to that, nor it will be fair to the one you will include as co-author if co-authorship includes processing/analyzing the data.The fairest thing to do is to mention the other student in the acknowledgements very clearly pointing out his/her actual participation.This is how I would solve this issue. On the other hand I have never consider as "co-author" of any of my papers any person whose only task was gathering information or following a field protocol to obtain data or make experiments.Ren? On Friday, August 19, 2016 3:50 PM, Jorge A. Santiago-Blay via Histonet wrote: Query on authorship Dear Colleagues: I am writing a small paper resulting from research done with two undergraduates many years ago (and, later on, involving several other colleagues using cutting-edge technology). As the results became obvious, both of the students agreed (orally, in person) with me that we should get the research published. As far as I remember, there was no email or letter documenting that and, there was no manuscript, only the data and the methods we were using. The problem: I have located one of the former students (now a researcher at a major research institution), who is excited about getting the research published, but not the second student. Question: How to handle the contribution (including authorship) of the other person? Here are some options I see. a. *Omit the name of the person that has not been located* and indicate that another person was involved in the data collection but we were hot able to locate him/her to get his/her approval to use his/her name as an author.? Under these circumstances, would it be OK to name the person in the Acknowledgments? Lately, I am asking permission to do that because sometimes some people prefer to remain anonymous. b. *Include the name of the person I cannot locate as an author*, an act of fairness and good faith on my part. If the person does not like the idea (and the paper is published) retract the name of the person in an erratum, later on, and assume responsibility for my error. A kind colleague did that to me once and, subsequently, it has resulted a long standing collaboration (and co-authorship in many papers, with my knowledge) :) c. *Nor use the data garnered by the person I cannot locate*. Although I am pretty sure I am authorized by the institution to use the data, as a general personal; preference, I like to ask permission. If you have something constructive to comment, kindly direct your comments to me, blayjorge at gmail.com , Apologies for potential cross-posting. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jclark at pcnm.com Mon Aug 22 15:05:00 2016 From: jclark at pcnm.com (Joanne Clark) Date: Mon, 22 Aug 2016 20:05:00 +0000 Subject: [Histonet] CAP ANP.22970 Query Message-ID: <7A7BDD92B984E847A7E71BC9C00A66D31277C620@S11MAILD034N2.sh11.lan> Hi Histonetters, we are wondering what everyone else out there is doing to be compliant with the following requirement? We do ER and PR by IHC but dont know what published benchmarks are out there to compare ourselves to. Also, how do you record interobserver variability amongst the pathologists? Any insights into this would be appreciated. ANP.22970 Annual Result Comparison Phase II For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. NOTE: Individuals interpreting the assay must also have their concordance compared with each other and this concordance should also be at least 95%. With specific reference to estrogen and progesterone receptor studies: in general, the overall proportion of ER-negative breast cancers (invasive and DCIS) should not exceed 30%. The proportion is somewhat lower in postmenopausal than premenopausal women (approximately 20% vs. 35%). The proportion is considerably lower in well-differentiated carcinomas (<10%) and certain special types of invasive carcinomas (<10% in lobular, tubular, and mucinous types). The proportion of PgR-negative cases is 10-15% higher than for ER-negative in each of these settings. Investigation is warranted if the proportion of negative cases is significantly lower in any of these settings. Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Roswell, New Mexico Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From tbraud at holyredeemer.com Tue Aug 23 14:06:50 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 23 Aug 2016 19:06:50 +0000 Subject: [Histonet] ER/PR benchmarks Message-ID: <48E053DDF6CE074DB6A7414BA05403F80865C8@HRHEX02-HOS.holyredeemer.local> The ER/PR benchmarks are those published in the notes section of the checklist question. We use the lowest numbers of the ranges. We track , patient age, cancer type, tumor grade, and positive or negative results, then just run the numbers. We also add in some extras, such as cases positive for one antibody, and negative for another, just for tracking purposes only. For Interobserver variability, at the advice of CAP, we simply allow each pathologist to independently read the CAP ER/PR (PMB) survey and record their answers. Those are compared with the correct answers and with each other. They must achieve <10% variability, or be enrolled in performance improvement until they can. I hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Tuesday, August 23, 2016 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 153, Issue 19 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. CAP ANP.22970 Query (Joanne Clark) ---------------------------------------------------------------------- Message: 1 Date: Mon, 22 Aug 2016 20:05:00 +0000 From: Joanne Clark To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] CAP ANP.22970 Query Message-ID: <7A7BDD92B984E847A7E71BC9C00A66D31277C620 at S11MAILD034N2.sh11.lan> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters, we are wondering what everyone else out there is doing to be compliant with the following requirement? We do ER and PR by IHC but dont know what published benchmarks are out there to compare ourselves to. Also, how do you record interobserver variability amongst the pathologists? Any insights into this would be appreciated. ANP.22970 Annual Result Comparison Phase II For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. NOTE: Individuals interpreting the assay must also have their concordance compared with each other and this concordance should also be at least 95%. With specific reference to estrogen and progesterone receptor studies: in general, the overall proportion of ER-negative breast cancers (invasive and DCIS) should not exceed 30%. The proportion is somewhat lower in postmenopausal than premenopausal women (approximately 20% vs. 35%). The proportion is considerably lower in well-differentiated carcinomas (<10%) and certain special types of invasive carcinomas (<10% in lobular, tubular, and mucinous types). The proportion of PgR-negative cases is 10-15% higher than for ER-negative in each of these settings. Investigation is warranted if the proportion of negative cases is significantly lower in any of these settings. Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Roswell, New Mexico Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 153, Issue 19 ***************************************** From Richard.Cartun at hhchealth.org Tue Aug 23 14:36:40 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 23 Aug 2016 19:36:40 +0000 Subject: [Histonet] CAP ANP.22970 Query In-Reply-To: <7A7BDD92B984E847A7E71BC9C00A66D31277C620@S11MAILD034N2.sh11.lan> References: <7A7BDD92B984E847A7E71BC9C00A66D31277C620@S11MAILD034N2.sh11.lan> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E9537D41B@HHCEXCHMB03.hhcsystem.org> Do you participate in the CAP's PT program for ER/PR and HER2 IHC testing? If so, you can have all your pathologists who interpret ER/PR/HER2 IHC testing score the PT TMAs, complete the worksheets, and then you can establish their interobserver variability. If not, pull 20 cases where ER/PR/HER2 was performed, prepare a score-sheet and have all your pathologists interpret these 20 cases. Run the same comparison. You will see ER positivity in the range of 75-90%, PR positivity in the range of 60-75%, and HER2 should be between 10-20%. Obviously, these ranges will depend on your patient demographics, and the antibody clones and IHC detection used. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Joanne Clark via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, August 22, 2016 4:05 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CAP ANP.22970 Query Hi Histonetters, we are wondering what everyone else out there is doing to be compliant with the following requirement? We do ER and PR by IHC but dont know what published benchmarks are out there to compare ourselves to. Also, how do you record interobserver variability amongst the pathologists? Any insights into this would be appreciated. ANP.22970 Annual Result Comparison Phase II For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory. NOTE: Individuals interpreting the assay must also have their concordance compared with each other and this concordance should also be at least 95%. With specific reference to estrogen and progesterone receptor studies: in general, the overall proportion of ER-negative breast cancers (invasive and DCIS) should not exceed 30%. The proportion is somewhat lower in postmenopausal than premenopausal women (approximately 20% vs. 35%). The proportion is considerably lower in well-differentiated carcinomas (<10%) and certain special types of invasive carcinomas (<10% in lobular, tubular, and mucinous types). The proportion of PgR-negative cases is 10-15% higher than for ER-negative in each of these settings. Investigation is warranted if the proportion of negative cases is significantly lower in any of these settings. Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Roswell, New Mexico Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Donna.Willis at BSWHealth.org Tue Aug 23 14:38:47 2016 From: Donna.Willis at BSWHealth.org (Willis, Donna G.) Date: Tue, 23 Aug 2016 19:38:47 +0000 Subject: [Histonet] Baylor Dallas Recuritment Event Message-ID: Baylor Scott&White North Texas will be hosting a Recruitment Event on August 27, 2016 from 10AM to 1PM. The Link to the Event flyer is below. http://bit.ly/BSWHLab_Rad_August2016 Thanks, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** The information contained in this e-mail may be privileged and/or confidential, and protected from disclosure, and no waiver of any attorney-client, work product, or other privilege is intended. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. The sender and Baylor Scott & White Health, and its affiliated entities, hereby expressly reserve all privileges and confidentiality that might otherwise be waived as a result of an erroneous or misdirected e-mail transmission. No employee or agent is authorized to conclude any binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, by e-mail without express written confirmation by the CEO, the Senior Vice President of Supply Chain Services or other duly authorized representative of Baylor Scott & White Health. From nowakc at comcast.net Tue Aug 23 18:53:10 2016 From: nowakc at comcast.net (nowakc) Date: Wed, 24 Aug 2016 02:53:10 +0300 Subject: [Histonet] I guess I've found it at last Message-ID: <00000cce342f$61b56200$f909e686$@comcast.net> Hey friend, As you know I've been looking for some suff for a long time, and I think I've found it at last, just take a look Faithfully, nowakc From lmarie08 at uga.edu Wed Aug 24 08:35:19 2016 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Wed, 24 Aug 2016 13:35:19 +0000 Subject: [Histonet] "Chore chart" Message-ID: Hello all in histoworld, I have been tasked with creating a "chore chart" of sorts to set up rotating division of duties in the lab. I was wondering if anyone else does this, and if they do could you take a picture of your chart and paste it in an email so I could see an example of one? Thanks! Lauren From LRaff at uropartners.com Wed Aug 24 08:45:21 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 24 Aug 2016 13:45:21 +0000 Subject: [Histonet] Landmark blog post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10F49C00@COLOEXCH01.uropartners.local> Hope you all don't mind. Some links to lab blog posts, some other things as well. 100th blog post Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From johnronan at custompathlabsolutions.com Wed Aug 24 09:17:02 2016 From: johnronan at custompathlabsolutions.com (John Ronan) Date: Wed, 24 Aug 2016 10:17:02 -0400 Subject: [Histonet] Battery for DRS-601 stainer In-Reply-To: References: Message-ID: Hi Histonetters, I have an old Sakura DRS-601 stainer and am getting a low voltage warning. I need to replace the battery that is used to save the staining programs. Does anyone know where the battery is located and where to get a replacement battery? John Ronan Custom Pathlab Solutions From scrochiere at nedlc.com Wed Aug 24 09:27:14 2016 From: scrochiere at nedlc.com (Steven Crochiere) Date: Wed, 24 Aug 2016 10:27:14 -0400 Subject: [Histonet] Quantifiable evaluation criteria Message-ID: <000001d1fe13$9c4a8c20$d4dfa460$@nedlc.com> How would one develop an evaluation standard for a "Lead Histo" that is more quantifiable rather than subjective criteria? I cannot see it as far as "leadership" skills. Any suggestions? Thanx, steve From Gail.Macke at cchmc.org Wed Aug 24 09:45:15 2016 From: Gail.Macke at cchmc.org (Macke, Gail) Date: Wed, 24 Aug 2016 14:45:15 +0000 Subject: [Histonet] I guess I've found it at last In-Reply-To: <00000cce342f$61b56200$f909e686$@comcast.net> References: <00000cce342f$61b56200$f909e686$@comcast.net> Message-ID: <5B3FA9A3-5C18-4AC7-BB9C-3C7D1476E73F@cchmc.org> Histonet, Received this today. What is this? Can you look into this? Has anyone else received this? Took off ?nowakc? from email address and Marcie DuVernay. Gail Macke,HTL On Aug 23, 2016, at 7:53 PM, nowakc via Histonet > wrote: Hey friend, As you know I've been looking for some suff for a long time, and I think I've found it at last, just take a look Faithfully, nowakc _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=CwICAg&c=P0c35rBvlN7D8BNx7kSJTg&r=EHoZsUPFU92qg6yiYwM5gy-CtQoI_1E8mAl9MzLu1wM&m=s0fBF1Btd-szh9j_wmmrKQU4gX_sfJpHFO2e0B9SJWw&s=bxGSTCFSkNOWyC0m8yWv2uI7goBINqTQ-e2IJvv60bw&e= From rjbuesa at yahoo.com Wed Aug 24 09:56:28 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 24 Aug 2016 14:56:28 +0000 (UTC) Subject: [Histonet] Quantifiable evaluation criteria In-Reply-To: <000001d1fe13$9c4a8c20$d4dfa460$@nedlc.com> References: <000001d1fe13$9c4a8c20$d4dfa460$@nedlc.com> Message-ID: <833886368.1596429.1472050588072@mail.yahoo.com> 1- Make a list of ALL the tasks you delegate on this "Lead Histo"2- Quantify each tasks, i.t. give a "numeric weight" to each? of a maximum 100 points.3- Keep track of how the "Lead Histo" performs in each and DISCUSS your evaluation with the "Histo Lead" quarterly. This will allow the "Lead Histo" know how you appreciate his work and which tasks need improvement.4- Come the annual evaluation just add-up all the points against a maximum allowable of 100% and you will get an unbiased/discussed evaluation of your "Lead Histo".That is how I used to do it.Ren? On Wednesday, August 24, 2016 10:36 AM, Steven Crochiere via Histonet wrote: How would one develop an evaluation standard for a "Lead Histo" that is more quantifiable rather than subjective criteria? I cannot see it as far as "leadership" skills. Any suggestions? Thanx, steve _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Wed Aug 24 10:00:40 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 24 Aug 2016 15:00:40 +0000 (UTC) Subject: [Histonet] I guess I've found it at last In-Reply-To: <5B3FA9A3-5C18-4AC7-BB9C-3C7D1476E73F@cchmc.org> References: <00000cce342f$61b56200$f909e686$@comcast.net> <5B3FA9A3-5C18-4AC7-BB9C-3C7D1476E73F@cchmc.org> Message-ID: <84295437.1460150.1472050840517@mail.yahoo.com> Yes, I received it.Most probably it is a disguised?"junk/spam" advertisement.Just in case do NOT open it.Ren? On Wednesday, August 24, 2016 10:58 AM, "Macke, Gail via Histonet" wrote: Histonet, Received this today. What is this? Can you look into this? Has anyone else received this? Took off ?nowakc? from email address and Marcie DuVernay. Gail Macke,HTL On Aug 23, 2016, at 7:53 PM, nowakc via Histonet > wrote: Hey friend, As you know I've? been looking for some? suff for a long time, and I think I've? found it at? last, just take? a look Faithfully, nowakc _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=CwICAg&c=P0c35rBvlN7D8BNx7kSJTg&r=EHoZsUPFU92qg6yiYwM5gy-CtQoI_1E8mAl9MzLu1wM&m=s0fBF1Btd-szh9j_wmmrKQU4gX_sfJpHFO2e0B9SJWw&s=bxGSTCFSkNOWyC0m8yWv2uI7goBINqTQ-e2IJvv60bw&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Linda.Margraf at cookchildrens.org Wed Aug 24 14:37:48 2016 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Wed, 24 Aug 2016 19:37:48 +0000 Subject: [Histonet] I guess I've found it at last (nowakc) Message-ID: <480957e573b54773a495ccd598760d53@MBX10.CCHCS.LDAP> Histonetters, I think this message below is SPAM of some sort and I recommend not opening the link. Several people have commented on it now. I have removed the person who sent it from the list. If that person was erroneously removed, they should let me know. I know people's email addresses get hijacked sometimes. Anyway, whenever an odd message like this with a link pops up, please don't open it. Thanks Linda M Histonet administrator Date: Wed, 24 Aug 2016 02:53:10 +0300 From: nowakc To: "Norton Renewals" , "histonet" , "histonet-request" , "Marcie DuVernay" Subject: [Histonet] I guess I've found it at last Message-ID: <00000cce342f$61b56200$f909e686$@comcast.net> Content-Type: text/plain; charset="us-ascii" Hey friend, As you know I've been looking for some suff for a long time, and I think I've found it at last, just take a look Faithfully, nowakc ************************************ From kim.p.kowsz at pfizer.com Wed Aug 24 19:06:53 2016 From: kim.p.kowsz at pfizer.com (Kowsz, Kim P) Date: Thu, 25 Aug 2016 00:06:53 +0000 Subject: [Histonet] can you list this on the histonet forum? Message-ID: <33396311FD13C145AC7F059E86E8E5E6592573CE@NDHAMREXDE06.amer.pfizer.com> I haven't listed in a long time (only reviewed posts). Would you kindly post the attached Job Posting? If I should approach the post in a different manner, I would appreciate direction. Thanks so much. Kim P Kowsz, BS, HT (ASCP), LAT (AALAS) Kim.p.kowsz at pfizer.com Pathology Laboratory Manager Pfizer, Inc., Drug Safety R&D MS 8274-1415 Eastern Point Rd Groton, CT 06340 860-715-5230 From kim.p.kowsz at pfizer.com Wed Aug 24 19:22:37 2016 From: kim.p.kowsz at pfizer.com (Kowsz, Kim P) Date: Thu, 25 Aug 2016 00:22:37 +0000 Subject: [Histonet] Exciting Career Opportunity Message-ID: <33396311FD13C145AC7F059E86E8E5E6592573F7@NDHAMREXDE06.amer.pfizer.com> Job Description Job Title : DSRD Pathology Technician (T2) - ASCP (HT) Job Id : 1032966 Locations : United States-Connecticut-Groton Please apply on-line at our website: www.pfizercareers.com. About Pfizer A career at Pfizer offers opportunity, ownership and impact. All over the world, Pfizer colleagues work together to positively impact health for everyone, everywhere. Our colleagues have the opportunity to grow and develop a career that offers both individual and company success; be part of an ownership culture that values diversity and where all colleagues are energized and engaged; and the ability to impact the health and lives of millions of people. Pfizer, a global leader in the biopharmaceutical industry, is continuously seeking top talent who are inspired by our purpose to innovate to bring therapies to patients that significantly improve their lives. Role Description The Pathology Technician is responsible for performing accurate, high quality necropsy/histology study work for rodent and non-rodent safety studies in compliance with the study protocol, Standard Operating Procedures (SOPs), Good Laboratory Practice (GLP) regulations, Environmental Health and Safety (EHS) standards, animal welfare regulations, and departmental policies/procedures. The pathology data collected and processed on these studies by the Pathology Technician enable candidate selection, first-in-human studies, 13-week studies and early research and target safety and investigative work. Responsibilities * Perform all regulatory responsibilities in compliance with applicable regulatory standards including animal welfare regulations. * Responsible for performing all aspects of the necropsy/histology phase of rodent and nonrodent safety studies including study set up, animal handling and anesthesia/euthanasia, sample collection (e.g. Blood, urine, CSF), necropsy, tissue trimming, processing, slide cutting, staining, data collection and documentation, data quality control review, and preparation of necropsy/histology data for archiving. * Read, understand and follow the study protocol and understand connection between study protocol and PDS protocol which is based on the study protocol. * Serve as Study Coordinator for studies depending on level and experience. * Begins to manage multiple assignments/projects simultaneously while maintaining data quality. Meet timelines under limited supervision. * Ensure proper supplies and equipment are available and suitable for study conduct functions; generate forms, labels, and other materials needed for study conduct as required. * Responsible for use of equipment, e.g. balances, tissue processors. * Communicate with Management, Study Director, Comparative Medicine and support personnel to ensure compliance with all protocol driven activities, high quality animal welfare practices are followed, and to enable proactive adjustment of the protocol by the Study Director in the event of unexpected events or findings. * Completes self review of necropsy/histology data for accuracy and completeness against the protocol; appropriately documents and corrects data errors, notifies appropriate study personnel as required. * May completes peer review of necropsy/histology data collected by others depending on experience and level. * May assist in preparing data for QA audits and addressing and drafting responses depending on level and experience. * Adheres to all applicable company and unit policies and procedures * Ensures work areas are kept clean and orderly. * Meets established timelines for deliverables. * May reviews and recommend updates for departmental SOPs, may draft updates to SOPs depending on experience and level. * Participates in a culture of continuous improvement within assigned work group. * Other activities as delegated by Test Facility Management. * Overtime, weekend and holiday work will be required. Qualifications * Strong functional/technical skills * Performs effectively as a team member; accurately records data; effectively follows verbal and written instructions; maintains a positive work atmosphere; communicates effectively; and interacts in a professional manner with management, colleagues, and customer and partner groups. * Strong organizational and time management skills, ability to handle multiple projects simultaneously while maintaining data quality and meeting timelines. * Ability to work effectively in a collaborative, team oriented environment and to meet study timelines. * Strong interpersonal and communication skills. * Demonstrates consistent knowledge of regulatory requirements such as GLP and animal welfare (eg. USDA) regulations. * Has technical knowledge of in vivo general toxicology safety studies. * Is multi-skilled and has a working knowledge of operations across the business to support collaborations with team groups and partner lines (e.g. Comparative Medicine, PDM, Clinical Pathology, In Vivo Toxicology, Formulations) etc. * Ability to conduct the necropsy/histology sections of moderately complex in vivo rodent and non-rodent studies. * Ability to handle multiple projects simultaneously, maintaining a high quality of data and timely delivery of results * Proficiency in laboratory equipment and data collection tools. * Ability to recognize, document and resolve data errors. * Preferred - HT Certification - Training in histology/necropsy and/or performing other laboratory techniques for in vivo studies in multiple species (rat, mouse, dog, NHP) in a GLP environment. - Use of electronic data capture systems, spreadsheet applications or other data management systems * High School with >5 years in animal facility; or Associates Degree (US) or equivalent in biology, veterinary technology, or related field with 2-5 years related experience; or * BS in biology or related field with 0-2 years related From mnesta.ebs at gmail.com Thu Aug 25 13:16:21 2016 From: mnesta.ebs at gmail.com (Michael Nesta) Date: Thu, 25 Aug 2016 14:16:21 -0400 Subject: [Histonet] Part-Time Histotech Opening in Richmond, VA Message-ID: GI Endoscopy Center requires HT(ASCP) Certified Technican to operate a new, low volume GI Lab. Flexible hours; friendly, low pressure work environment; bright, clean, new Lab and excellent compensation. A great 2nd job opportunity at 8 - 12 hours/week. If interested, please forward Resume and Cover Letter to LNesta at ebsciences.com Thank you, Michael Nesta President Energy Beam Sciences, Inc. mnesta at ebsciences.com From JMaslanka at stpetes.org Thu Aug 25 13:24:35 2016 From: JMaslanka at stpetes.org (Joseph Maslanka) Date: Thu, 25 Aug 2016 12:24:35 -0600 Subject: [Histonet] CPT? Message-ID: Greetings from Big Sky Country, in the near future a genetic laboratory will begin requesting tissue samples from us ( cutting 20 micron sections placed in a micro-tubes ) for their testing. My question is, is there any other CPT codes besides 88363 to get reimbursed for our time and effort providing these samples? Thanks Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. Communication of electronic protected health information (ePHI) is protected under the Health Insurance Portability and Accountability Act (HIPAA) Act of 1996. Electronic mail (e-mail) communication is not encrypted or secure. The HIPAA Security Rule allows for patients to initiate communication of personal health information over this medium and for providers to respond accordingly with the understanding that privacy of communication is not guaranteed. From LRaff at uropartners.com Thu Aug 25 13:42:48 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 25 Aug 2016 18:42:48 +0000 Subject: [Histonet] CPT? In-Reply-To: References: Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10F4C855@COLOEXCH01.uropartners.local> Is the genetics company billing the patient/insurance for clinical testing of the samples? If so, your lab can establish a contractual arrangement by which the genetics lab can pay your lab fair market value for the time and labor to provide the specimen. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: Joseph Maslanka via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, August 25, 2016 1:25 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CPT? Greetings from Big Sky Country, in the near future a genetic laboratory will begin requesting tissue samples from us ( cutting 20 micron sections placed in a micro-tubes ) for their testing. My question is, is there any other CPT codes besides 88363 to get reimbursed for our time and effort providing these samples? Thanks Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things..... "Kindness is the language the blind can see & the deaf can hear- Mark Twain This electronic mail message contains information which is confidential. If you are not the intended recipient, please be aware that any disclosure, photocopying, distribution or use of the contents of the received information is prohibited. If you have received this e-mail in error, please reply to the sender immediately and permanently delete this message and all copies of it. Thank you. Communication of electronic protected health information (ePHI) is protected under the Health Insurance Portability and Accountability Act (HIPAA) Act of 1996. Electronic mail (e-mail) communication is not encrypted or secure. The HIPAA Security Rule allows for patients to initiate communication of personal health information over this medium and for providers to respond accordingly with the understanding that privacy of communication is not guaranteed. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Albert.Santiago at uphs.upenn.edu Thu Aug 25 14:23:58 2016 From: Albert.Santiago at uphs.upenn.edu (Santiago, Albert) Date: Thu, 25 Aug 2016 19:23:58 +0000 Subject: [Histonet] Gamma/Delta IHC staining using paraffin tissue Message-ID: Hello fellow histonetters, I wanted to know if anyone out there is using a Gamma/Delta antibody for IHC staining using paraffin embedded tissue. I would like to work one up and I would like some guidance as far as the antibody is concerned and procedure. Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market ST. Ste 201 Philadelphia, PA 19104 215-662-6539 - Lab 215-662-6759-office albert.santiago at uphs.upenn.edu From gagnone at KGH.KARI.NET Thu Aug 25 14:44:39 2016 From: gagnone at KGH.KARI.NET (Gagnon, Eric) Date: Thu, 25 Aug 2016 19:44:39 +0000 Subject: [Histonet] automated special stains and control tissue Message-ID: <5F06C3AD0B27264CA20CFA986C87882E01BB65ED3A@EXCHANGEPV1.KGH.ON.CA> Agree with your opinion re: including special stain control tissue and patient tissue on same slide, Curt. Assuming you're referring to FFPE tissue, there is little if any chance of the control tissue contaminating the patient tissue on the same slide, thus causing a 'false positive' in the patient tissue. (Even if it did, a good pathologist could tell it was not native tissue to that section and was sitting 'on top' of the patient section :) Including control and patient on the same slide for automated special staining IS a process change and may take some time, commitment and training to implement, but for the same reasons that it makes sense in IHC, it makes sense in automated special staining. A side benefit to confidence in the correct dispensing of reagent on each slide is the saving in slides, reagents, and room on your stainer that fewer independent control slides would represent. Perhaps try some test runs to instill confidence in this process change before implementing. Eric Gagnon, MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From mnesta.ebs at gmail.com Thu Aug 25 15:18:51 2016 From: mnesta.ebs at gmail.com (Michael Nesta) Date: Thu, 25 Aug 2016 16:18:51 -0400 Subject: [Histonet] Follow-Up to Part Time Histotech Opening Message-ID: The location of the Part-Time Tech opening I posted earlier is Richmond, VA, which is noted in the subject line of the post. Michael Nesta Energy Beam Sciences, Inc. From rennie1108 at yahoo.com Fri Aug 26 10:10:57 2016 From: rennie1108 at yahoo.com (Adrienne Anderson) Date: Fri, 26 Aug 2016 15:10:57 +0000 (UTC) Subject: [Histonet] Multiplex IF staining References: <1354934068.231551.1472224257270.ref@mail.yahoo.com> Message-ID: <1354934068.231551.1472224257270@mail.yahoo.com> Hello Hist-world, Does anyone have experience doing FISH/double IF staining? My newest project is to combine a FISH probe (texas red) with anti-Staufen IF (green secondary) and anti-MBNL1 IF (secondary TBD). So I've got a red, green, and blue (DAPI counterstain) already. If I used a yellow secondary for the MBNL1, would you be able to see the difference between that and the green? I'm running out of colors here! Any help would be greatly appreciated. Thanks so much,Adrienne From tkngflght at yahoo.com Fri Aug 26 20:51:44 2016 From: tkngflght at yahoo.com (Cheryl) Date: Fri, 26 Aug 2016 18:51:44 -0700 Subject: [Histonet] How and genetics - Message-ID: Hi Joe-- are these research protocols or fda approved tests? If you're not charging the patient's insurance you're not restricted to CPT codes for billing. You can CLIENT bill them a reasonable 'per curl' charge or you could bill the handling charge and release the block so they can cut their own. Consider how clean you have to keep everything for this-- a couple of transferred cells and ... We require patient signature or signed clinician orders from the requesting entity and release the block with really really detailed tracking including scans of the release document saved with the primary case. Hope this helps! Cheryl From Ronald.Houston at nationwidechildrens.org Tue Aug 30 06:42:10 2016 From: Ronald.Houston at nationwidechildrens.org (Houston, Ronald) Date: Tue, 30 Aug 2016 11:42:10 +0000 Subject: [Histonet] pediatric facilities only Message-ID: If you do not work in a pediatric facility please disregard I need some help getting statistics for benchmarking for staffing evaluation What is your annual volume of cases/blocks? Continual flow processing? # of IHC/Special stains EM? Shifts? Days only? Weekend coverage? How many HTs do you have? How many PAs? Lab Assistants? How many pathologists? Do you perform any research? What do you do regarding transcription? # of transcriptionists, outsource, voice recognition? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC FIBMS Anatomic Pathology Manager Laboratory Services 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston at nationwidechildrens.org www.NationwideChildrens.org "Without continual growth and progress, such words as improvement, achievement, and success have no meaning." ~ Ben Franklin From ecameron1 at midcoasthealth.com Tue Aug 30 09:25:24 2016 From: ecameron1 at midcoasthealth.com (Cameron, Elizabeth) Date: Tue, 30 Aug 2016 14:25:24 +0000 Subject: [Histonet] Eosin Message-ID: Hi, I have been staining fish tissues fixed in Davidsons with H&E, and the researcher would like the eosin to be more intense. Our standard protocol works well for our own tissue, but the fish look much more washed out. I am using alcoholic eosin Y, have tried both water and alcohol before and I have varied the alcohol differentiation steps after the Eosin. I also extended the time in Eosin and increased the wash after bluing to make sure the sections are not basic. Any suggestions would be appreciated. Thank you. Elizabeth M. Cameron, HT(ASCP), QIHCCM Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 From SteveM at mcclainlab.com Tue Aug 30 13:14:24 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Tue, 30 Aug 2016 18:14:24 +0000 Subject: [Histonet] Histonet Digest, Vol 153, Issue 24 davidsons fixative fish In-Reply-To: References: Message-ID: Something sounds fishy Dr. Richmond can solve it. http://www.ihcworld.com/_protocols/histology/davidson_fixative.htm Steve A. McClain, MD On Aug 30, 2016, at 13:22, "histonet-request at lists.utsouthwestern.edu" > wrote: Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. pediatric facilities only (Houston, Ronald) 2. Eosin (Cameron, Elizabeth) ---------------------------------------------------------------------- Message: 1 Date: Tue, 30 Aug 2016 11:42:10 +0000 From: "Houston, Ronald" > To: "histonet at lists.utsouthwestern.edu" > Subject: [Histonet] pediatric facilities only Message-ID: > Content-Type: text/plain; charset="us-ascii" If you do not work in a pediatric facility please disregard I need some help getting statistics for benchmarking for staffing evaluation What is your annual volume of cases/blocks? Continual flow processing? # of IHC/Special stains EM? Shifts? Days only? Weekend coverage? How many HTs do you have? How many PAs? Lab Assistants? How many pathologists? Do you perform any research? What do you do regarding transcription? # of transcriptionists, outsource, voice recognition? Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC FIBMS Anatomic Pathology Manager Laboratory Services 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston at nationwidechildrens.org www.NationwideChildrens.org "Without continual growth and progress, such words as improvement, achievement, and success have no meaning." ~ Ben Franklin ------------------------------ Message: 2 Date: Tue, 30 Aug 2016 14:25:24 +0000 From: "Cameron, Elizabeth" > To: "histonet at lists.utsouthwestern.edu" > Subject: [Histonet] Eosin Message-ID: > Content-Type: text/plain; charset="us-ascii" Hi, I have been staining fish tissues fixed in Davidsons with H&E, and the researcher would like the eosin to be more intense. Our standard protocol works well for our own tissue, but the fish look much more washed out. I am using alcoholic eosin Y, have tried both water and alcohol before and I have varied the alcohol differentiation steps after the Eosin. I also extended the time in Eosin and increased the wash after bluing to make sure the sections are not basic. Any suggestions would be appreciated. Thank you. Elizabeth M. Cameron, HT(ASCP), QIHCCM Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 153, Issue 24 ***************************************** From JMacDonald at mtsac.edu Tue Aug 30 13:09:00 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Tue, 30 Aug 2016 11:09:00 -0700 Subject: [Histonet] Eosin In-Reply-To: References: Message-ID: Elizabeth, You can try to lower the pH of the eosin a bit. Add some acetic acid. Use absolute alcohol after the eosin for differentiating/dehydrating. Jennifer From: "Cameron, Elizabeth via Histonet" To: "histonet at lists.utsouthwestern.edu" Date: 08/30/2016 07:29 AM Subject: [Histonet] Eosin Hi, I have been staining fish tissues fixed in Davidsons with H&E, and the researcher would like the eosin to be more intense. Our standard protocol works well for our own tissue, but the fish look much more washed out. I am using alcoholic eosin Y, have tried both water and alcohol before and I have varied the alcohol differentiation steps after the Eosin. I also extended the time in Eosin and increased the wash after bluing to make sure the sections are not basic. Any suggestions would be appreciated. Thank you. Elizabeth M. Cameron, HT(ASCP), QIHCCM Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ewj at pigs.ag Tue Aug 30 18:42:40 2016 From: ewj at pigs.ag (=?UTF-8?B?RS4gV2F5bmUgSm9obnNvbiDmnLHnqLPmo67ljZrlo6s=?=) Date: Wed, 31 Aug 2016 07:42:40 +0800 Subject: [Histonet] Eosin In-Reply-To: References: Message-ID: You might try some combination of eosin and biebrich scarlet and/or phloxine. Adding a little acetic acid might help. http://www.stainsfile.info/StainsFile/downloads/eosin.pdf I am quite fond of Biebrich Scarlet and like way it stains brain and heart particularly. Biebrich is much more red than eosin. Phloxine is an aggressive pink. * A bit punny about the fish looking washed out. * "There was something fishy about the butler. Probably a Pisces working for scale." - Phil Proctor On 08/30/2016 10:25 PM, Cameron, Elizabeth wrote: > Hi, > I have been staining fish tissues fixed in Davidsons with H&E, and the researcher would like the eosin to be more intense. Our standard protocol works well for our own tissue, but the fish look much more washed out. I am using alcoholic eosin Y, have tried both water and alcohol before and I have varied the alcohol differentiation steps after the Eosin. I also extended the time in Eosin and increased the wash after bluing to make sure the sections are not basic. Any suggestions would be appreciated. > Thank you. > > Elizabeth M. Cameron, HT(ASCP), QIHCCM > Lead Histologist > Mid Coast Hospital > 123 Medical Center Drive > Brunswick, ME 04011 > (207) 373-6573 > > -- E. Wayne Johnson ????? Enable AgTech Consulting ????????????? Beijing 188 1088 3205 From d.a.faichney at stir.ac.uk Wed Aug 31 05:53:38 2016 From: d.a.faichney at stir.ac.uk (Debbie Faichney) Date: Wed, 31 Aug 2016 10:53:38 +0000 Subject: [Histonet] Eosin In-Reply-To: References: Message-ID: <5bcade08b0e7486795f86711c1a8a58e@havra.ad.stir.ac.uk> Hi Elizabeth, We are an aquaculture histology laboratory and routinely use four parts 1% Eosin Y (aq) to one part Putts eosin for the same reason that you describe. 1% aqueous or alcoholic alone just doesn't do the job. Debbie Faichney, BSc Senior Technician Histology/Bacteriology Laboratories Institute of Aquaculture University of Stirling Stirling, FK9 4LA Scotland UK -----Original Message----- From: Cameron, Elizabeth via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: 30 August 2016 15:25 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Eosin Hi, I have been staining fish tissues fixed in Davidsons with H&E, and the researcher would like the eosin to be more intense. Our standard protocol works well for our own tissue, but the fish look much more washed out. I am using alcoholic eosin Y, have tried both water and alcohol before and I have varied the alcohol differentiation steps after the Eosin. I also extended the time in Eosin and increased the wash after bluing to make sure the sections are not basic. Any suggestions would be appreciated. Thank you. Elizabeth M. Cameron, HT(ASCP), QIHCCM Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The University achieved an overall 5 stars in the QS World University Rankings 2015 The University of Stirling is a charity registered in Scotland, number SC 011159. From SRagnaut at northwell.edu Wed Aug 31 10:20:47 2016 From: SRagnaut at northwell.edu (Ragnauth, Shrutikant) Date: Wed, 31 Aug 2016 11:20:47 -0400 Subject: [Histonet] PLA2R BY IHC Message-ID: Does anyone know of a lab that performs PLA2R by IHC testing? The information contained in this electronic e-mail transmission and any attachments are intended only for the use of the individual or entity to whom or to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this communication is not the intended recipient, or the employee or agent responsible for delivering this communication to the intended recipient, you are hereby notified that any dissemination, distribution, copying or disclosure of this communication and any attachment is strictly prohibited. If you have received this transmission in error, please notify the sender immediately by telephone and electronic mail, and delete the original communication and any attachment from any computer, server or other electronic recording or storage device or medium. Receipt by anyone other than the intended recipient is not a waiver of any attorney-client, physician-patient or other privilege. From Timothy.Morken at ucsf.edu Wed Aug 31 14:35:07 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 31 Aug 2016 19:35:07 +0000 Subject: [Histonet] Kaiser Sunnyside, Clackamas OR contact? Message-ID: <761E2B5697F795489C8710BCC72141FF6FD83746@ex07.net.ucsf.edu> All knowing histonet. Does anyone have a lab contact at Kaiser Sunnyside, Clackamas, OR? Their bureaucracy is impenetrable!! Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From tkngflght at yahoo.com Wed Aug 31 14:42:55 2016 From: tkngflght at yahoo.com (Cheryl) Date: Wed, 31 Aug 2016 19:42:55 +0000 (UTC) Subject: [Histonet] Two week temp -- full travel References: <305414220.2983208.1472672575792.ref@mail.yahoo.com> Message-ID: <305414220.2983208.1472672575792@mail.yahoo.com> Hi Guys- Fully funded travel position-- it's short but starts next week. ?Two weeks in duration. ?All expenses paid. ?Early AM private lab. ?Embed cut stain. If you're interested-- shoot me a copy of your most recent resume with a phone number and I'll call you with the 'skinny'. ?Need at least two years recent experience and high preference for small biopsy work.? Love this lab-- one of my favorite places to work!! Cheryl?Cheryl Kerry, HT(ASCP) Full Staff Inc. ? admin at fullstaff.org?800.756.3309 Phone & Fax https://www.facebook.com/TheHistologyCompany/ From deb.vaneyck at phci.org Wed Aug 31 14:47:13 2016 From: deb.vaneyck at phci.org (Van Eyck, Deb) Date: Wed, 31 Aug 2016 19:47:13 +0000 Subject: [Histonet] Claudin 4 immunostain Message-ID: Hi, Just looking for some information. Are any of you using a Claudin-4 immunostain along with or in place of ber-ep4, and or moc 31 on cytology fluid specimens? It is supposed to be more superior that the older 2 stains in differentiating mesothelial cells from adenocarcinomas in Cytology fluids. Thanks Deb ______________________________________________________________________ This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. ______________________________________________________________________ From rsrichmond at gmail.com Wed Aug 31 15:16:13 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Wed, 31 Aug 2016 16:16:13 -0400 Subject: [Histonet] Eosin Message-ID: Elizabeth M. Cameron, HT(ASCP), QIHCCM. Lead Histologist at Mid Coast Hospital in Brunswick, Maine asks: >>I have been staining fish tissues fixed in Davidson's fixative with H&E, and the researcher would like the eosin to be more intense. Our standard protocol works well for our own tissue, but the fish look much more washed out. I am using alcoholic eosin Y, have tried both water and alcohol before and I have varied the alcohol differentiation steps after the Eosin. I also extended the time in Eosin and increased the wash after bluing to make sure the sections are not basic. Any suggestions would be appreciated.<< Users vary a lot in how much eosin they want. Sometimes blends with other red dyes are preferable. When I was a resident at Johns Hopkins around 1970 our notoriously lurid eosin was compounded as follows: Eosin Y (C.I. 45380) 3.6 g Phloxine B (C.I. 45410) 1.5 g Biebrich scarlet (C.I. 26905 ) 0.3 g absolute alcohol 150 mL distilled water 120 mL Shake, or stir with a magnetic stirrer to dissolve, then add 450 mL more of distilled water. We used a lot of Davidson's fixative at JHH. As far as I know this formula was never published, though I've posted it online more than once, probably on Histonet. One of many formulas I made off with before I finished residency. Bob Richmond Samurai Pathologist Maryville TN From lmdee1 at yahoo.com Wed Aug 31 15:20:26 2016 From: lmdee1 at yahoo.com (Linda) Date: Wed, 31 Aug 2016 20:20:26 +0000 (UTC) Subject: [Histonet] Two week temp -- full travel In-Reply-To: <305414220.2983208.1472672575792@mail.yahoo.com> References: <305414220.2983208.1472672575792.ref@mail.yahoo.com> <305414220.2983208.1472672575792@mail.yahoo.com> Message-ID: <178312615.2821504.1472674826984@mail.yahoo.com> Hi Cheryl, I am attaching a copy of my resume for the information on the assignment.? I look forward to discussing the assignment. Regards, Linda Dee, BGS, HT(ASCP)847-431-3376 From: Cheryl via Histonet To: Histonet Sent: Wednesday, August 31, 2016 2:42 PM Subject: [Histonet] Two week temp -- full travel Hi Guys- Fully funded travel position-- it's short but starts next week. ?Two weeks in duration. ?All expenses paid. ?Early AM private lab. ?Embed cut stain. If you're interested-- shoot me a copy of your most recent resume with a phone number and I'll call you with the 'skinny'. ?Need at least two years recent experience and high preference for small biopsy work.? Love this lab-- one of my favorite places to work!! Cheryl?Cheryl Kerry, HT(ASCP) Full Staff Inc. ? admin at fullstaff.org?800.756.3309 Phone & Fax https://www.facebook.com/TheHistologyCompany/ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lmdee1 at yahoo.com Wed Aug 31 15:44:49 2016 From: lmdee1 at yahoo.com (Linda) Date: Wed, 31 Aug 2016 20:44:49 +0000 (UTC) Subject: [Histonet] Two week temp -- full travel-mistake In-Reply-To: <178312615.2821504.1472674826984@mail.yahoo.com> References: <305414220.2983208.1472672575792.ref@mail.yahoo.com> <305414220.2983208.1472672575792@mail.yahoo.com> <178312615.2821504.1472674826984@mail.yahoo.com> Message-ID: <1684778161.2877268.1472676289321@mail.yahoo.com> Please disregard my post.? I apologize.? I didn't realize I was answering the community. Thank you. From: Linda via Histonet To: Cheryl ; Histonet Sent: Wednesday, August 31, 2016 3:20 PM Subject: Re: [Histonet] Two week temp -- full travel Hi Cheryl, I am attaching a copy of my resume for the information on the assignment.? I look forward to discussing the assignment. Regards, Linda Dee, BGS, HT(ASCP)847-431-3376 ? ? ? From: Cheryl via Histonet To: Histonet Sent: Wednesday, August 31, 2016 2:42 PM Subject: [Histonet] Two week temp -- full travel ? Hi Guys- Fully funded travel position-- it's short but starts next week. ?Two weeks in duration. ?All expenses paid. ?Early AM private lab. ?Embed cut stain. If you're interested-- shoot me a copy of your most recent resume with a phone number and I'll call you with the 'skinny'. ?Need at least two years recent experience and high preference for small biopsy work.? Love this lab-- one of my favorite places to work!! Cheryl?Cheryl Kerry, HT(ASCP) Full Staff Inc. ? admin at fullstaff.org?800.756.3309 Phone & Fax https://www.facebook.com/TheHistologyCompany/ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alamberth at lji.org Wed Aug 31 20:24:15 2016 From: alamberth at lji.org (Angela Lamberth) Date: Wed, 31 Aug 2016 18:24:15 -0700 Subject: [Histonet] Modified Movat's Pentachrome stain Message-ID: Hi Histonetters! I?m gearing up to perform a pentachrome stain. I will be making this in house and not using a kit. Through searching histonet, I?ve found a protocol used by the Children?s Hospital of Philadelphia Pathology Core. http://pathcore.research.chop.edu/docs/MovatPentachromeStain.pdf Two things: Iodine crystals are out of the question. Can I replace that with Lugol?s iodine solution or should I just substitute Weigert?s for the hematoxylin in this protocol? Also, saffron vs tartrazine vs Orange G stain. There is a real difference in price. Does anybody have a personal preference in terms of quality or aesthetics? Is paying the extra money for saffron really worth it? Thanks! Angela -- Angela Lamberth Histology Technician II Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 From jobposting2600 at gmail.com Wed Aug 31 20:41:16 2016 From: jobposting2600 at gmail.com (Practice Manager) Date: Wed, 31 Aug 2016 18:41:16 -0700 Subject: [Histonet] Histotechnician Position - Chandler, AZ Message-ID: <10E67FBB-259C-4FAF-AA32-308CF7A7D945@gmail.com> HISTOTECHNICIAN Chandler Pathology Services - Chandler, AZ Job Type: Full-time Reports to: Laboratory Manager Schedule: 40 Hours per Week Salary: DOE FLSA Status: Hourly; Non-Exempt Essential functions and responsibilities: ? Ensures compliance with all local, federal, CLIA, regulations. ? Performs routine and non-routine activities involved in the preparation of slides for microscopic evaluation by pathologist(s), according to policies and procedures. ? Responsible for embedding of surgical pathology specimens. ? Responsible for the sectioning and microtomy of surgical pathology specimens. ? Responsible for the routine staining, histochemical staining, and immunohistochemical staining of surgical pathology specimens for microscope evaluation and analysis. ? Performs routine maintenance and cleaning of equipment and troubleshoots minor equipment failures. Documents remedial actions such as repairs or repeated tests. ? Adheres to laboratory?s quality control policies and documents all quality control activities. ? Maintains diagnostic viability of all specimens and ensures correct patient labeling. ? Unpacks orders received; dates and stocks orders, in accordance with established policies and procedures. ? Maintain a clean and safe prep work area in accordance with all laboratory and safety SOP?s. ? Prepares and labels necessary stains and reagents in accordance with departmental procedures, policies and standards. ? Reviews and performs QC on slides before taking the slides to the pathologist. ? Maintenance of accurate work records and logs (i.e., Discrepancy Logs, Maintenance Logs, Quality Control Logs etc.) ? Demonstrates a commitment to service, organizational values, and professionalism through appropriate conduct and demeanor at all times. ? Works collaboratively and supports efforts of team members. ? Protects patient information by adhering to professional standards including the Health Information Portability and Accountability Act (HIPAA). Knowledge and Skill: ? Must have 2+ years laboratory experience. ? Must be HT Certified. ? Must be precise when performing technical tasks. ? Effective interpersonal skills, both in person and on the telephone. ? High accuracy in work and attention to detail. Physical demands: ? Must have manual dexterity and motor coordination. ? May involve sitting, standing, walking for long periods of time and/ or sitting at the microtome for long periods of time. ? Exposure to chemicals and fumes. Benefit Package: ? Health, Dental and Vision Insurance, 401K, Paid time off and Holiday Pay. Required education: ? Associate?s Degree or higher Required experience and certification: ? laboratory: 2 years ? HT Certification ASCP Chandler Pathology Services is an Equal Opportunity Employer. This company does not and will not discriminate in employment and personnel practices on the basis of race, sex, age, handicap, religion, national origin or any other basis prohibited by applicable law. Hiring, transferring and promotion practices are performed without regard to the above listed items. To apply, please fax resume to (480) 786-6996, ATTN: Office Manager or email: jobposting2600 at gmail.com