From LRaff at uropartners.com Fri Apr 1 09:48:59 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 1 Apr 2016 14:48:59 +0000 Subject: [Histonet] Just an April 1, Friday Fun blog post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B7B4895@COLOEXCH01.uropartners.local> http://www.chicagonow.com/downsize-maybe/2016/04/an-icon-abandoned-and-its-no-april-fool-prank/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From nlinke at sbch.org Fri Apr 1 10:42:18 2016 From: nlinke at sbch.org (Noelle Linke) Date: Fri, 1 Apr 2016 15:42:18 +0000 Subject: [Histonet] tests on old cases Message-ID: Hi all, We have been getting a lot of requests for tests such as Lynch syndrome on old hospital cases. How do all of you handle the billing on these? Do you ask the clinician to send updated insurance information and then log in a new case? Thank you, No?lle No?lle Linke, MS, HTL(ASCP) QIHC Manager, Anatomic Pathology Pacific Diagnostic Laboratories nlinke at sbch.org Phone: (805) 324-9814 Fax: (805) 696-6433 ________________________________ CH Disclaimer: This electronic mail message is intended exclusively for the individual or entity to which it is addressed. This message, together with any attachment, may contain confidential and privileged information. Any views, opinions or conclusions expressed in this message are those of the individual sender and do not necessarily reflect the views of Cottage Health, its subsidiaries or affiliates. This document may also contain information covered under the Health Insurance Portability and Accountability Act (HIPAA, PL 104-191) and implementing regulations and must be protected in accordance with those provisions. Re-disclosure without patient consent or as otherwise permitted by law is prohibited. Any unauthorized review, retransmission, use, printing, copying, retention, disclosure, distribution or the taking of any action in reliance upon this information by persons or entities other than the intended recipient is strictly prohibited and may be unlawful. If you have received this message in error, please immediately advise the sender by reply email message to the sender and delete all copies of this message from your system without copying. ________________________________ From MMargiotta at bmhmc.org Fri Apr 1 11:41:21 2016 From: MMargiotta at bmhmc.org (Margiotta-Watz, Michele) Date: Fri, 1 Apr 2016 16:41:21 +0000 Subject: [Histonet] bx levels Message-ID: <230D0B9EC57D7A45A7A186C6AB4C7ABC6DA266F4@BMH-EXCHANGE-01.BMHMC.ORG> Hi All, We are wondering how many levels most hospitals are cutting on GI biopsies? We currently cut 3 levels, thinking that is the standard of care. Please let us know how many are done at other hospitals. Thanks for your help! Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Road Patchogue, NY 11772 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From Richard.Cartun at hhchealth.org Fri Apr 1 11:59:35 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 1 Apr 2016 16:59:35 +0000 Subject: [Histonet] tests on old cases In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B0BE831@HHCEXCHMB03.hhcsystem.org> If the request is received more than "30" days from the original date-of-service, we re-accession the specimen, and bill and report the test result(s) under the new accession number. The requesting MD now becomes the submitting MD. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Noelle Linke via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, April 01, 2016 11:42 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] tests on old cases Hi all, We have been getting a lot of requests for tests such as Lynch syndrome on old hospital cases. How do all of you handle the billing on these? Do you ask the clinician to send updated insurance information and then log in a new case? Thank you, No?lle No?lle Linke, MS, HTL(ASCP) QIHC Manager, Anatomic Pathology Pacific Diagnostic Laboratories nlinke at sbch.org Phone: (805) 324-9814 Fax: (805) 696-6433 ________________________________ CH Disclaimer: This electronic mail message is intended exclusively for the individual or entity to which it is addressed. This message, together with any attachment, may contain confidential and privileged information. Any views, opinions or conclusions expressed in this message are those of the individual sender and do not necessarily reflect the views of Cottage Health, its subsidiaries or affiliates. This document may also contain information covered under the Health Insurance Portability and Accountability Act (HIPAA, PL 104-191) and implementing regulations and must be protected in accordance with those provisions. Re-disclosure without patient consent or as otherwise permitted by law is prohibited. Any unauthorized review, retransmission, use, printing, copying, retention, disclosure, distribution or the taking of any action in reliance upon this information by persons or entities other than the intended recipient is strictly prohibited and may be unlawful. If you have received this message in error, please immediately advise the sender by reply email message to the sender and delete all copies of this message from your system without copying. ________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LNormington at uwhealth.org Fri Apr 1 12:00:42 2016 From: LNormington at uwhealth.org (Normington Lacy) Date: Fri, 1 Apr 2016 17:00:42 +0000 Subject: [Histonet] bx levels In-Reply-To: <230D0B9EC57D7A45A7A186C6AB4C7ABC6DA266F4@BMH-EXCHANGE-01.BMHMC.ORG> References: <230D0B9EC57D7A45A7A186C6AB4C7ABC6DA266F4@BMH-EXCHANGE-01.BMHMC.ORG> Message-ID: <7F7174244DDD1A49BFBEE8A845BC2F2B19ECCA@UWHC-MBX05.uwhis.hosp.wisc.edu> We take four levels with 20 microns between each level. All four sections are placed on one single slide. Lacy Normington, HTL(ASCP)CM Manager, Surgical Pathology Lab Services UW Health 600 Highland Avenue Madison, WI 53792-2472 Phone: 608-890-9373 -----Original Message----- From: Margiotta-Watz, Michele via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, April 01, 2016 11:41 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] bx levels Hi All, We are wondering how many levels most hospitals are cutting on GI biopsies? We currently cut 3 levels, thinking that is the standard of care. Please let us know how many are done at other hospitals. Thanks for your help! Michele Margiotta-Watz Histology Supervisor BMHMC 101 Hospital Road Patchogue, NY 11772 631-654-7192 DISCLAIMER: This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LNormington at uwhealth.org Fri Apr 1 12:08:02 2016 From: LNormington at uwhealth.org (Normington Lacy) Date: Fri, 1 Apr 2016 17:08:02 +0000 Subject: [Histonet] tests on old cases In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E6B0BE831@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E6B0BE831@HHCEXCHMB03.hhcsystem.org> Message-ID: <7F7174244DDD1A49BFBEE8A845BC2F2B19ECED@UWHC-MBX05.uwhis.hosp.wisc.edu> At our institution, if the request is received more than "30" days from the collect date, we create a new HOV for the patient. That way the addendum is still on the same case number. The collect date is changed by our LIS department to be the "request to pull from archive" based on Medicare requirements. We add the requesting physician to the existing case. Lacy Normington, HTL(ASCP)CM Manager, Surgical Pathology Lab Services UW Health 600 Highland Avenue Madison, WI 53792-2472 Phone: 608-890-9373 -----Original Message----- From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, April 01, 2016 12:00 PM To: Noelle Linke Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] tests on old cases If the request is received more than "30" days from the original date-of-service, we re-accession the specimen, and bill and report the test result(s) under the new accession number. The requesting MD now becomes the submitting MD. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Noelle Linke via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, April 01, 2016 11:42 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] tests on old cases Hi all, We have been getting a lot of requests for tests such as Lynch syndrome on old hospital cases. How do all of you handle the billing on these? Do you ask the clinician to send updated insurance information and then log in a new case? Thank you, No?lle No?lle Linke, MS, HTL(ASCP) QIHC Manager, Anatomic Pathology Pacific Diagnostic Laboratories nlinke at sbch.org Phone: (805) 324-9814 Fax: (805) 696-6433 ________________________________ CH Disclaimer: This electronic mail message is intended exclusively for the individual or entity to which it is addressed. This message, together with any attachment, may contain confidential and privileged information. Any views, opinions or conclusions expressed in this message are those of the individual sender and do not necessarily reflect the views of Cottage Health, its subsidiaries or affiliates. This document may also contain information covered under the Health Insurance Portability and Accountability Act (HIPAA, PL 104-191) and implementing regulations and must be protected in accordance with those provisions. Re-disclosure without patient consent or as otherwise permitted by law is prohibited. Any unauthorized review, retransmission, use, printing, copying, retention, disclosure, distribution or the taking of any action in reliance upon this information by persons or entities other than the intended recipient is strictly prohibited and may be unlawful. If you have received this message in error, please immediately advise the sender by reply email message to the sender and delete all copies of this message from your system without copying. ________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jvickroy at SpringfieldClinic.com Fri Apr 1 12:18:27 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Fri, 1 Apr 2016 17:18:27 +0000 Subject: [Histonet] New Peloris Message-ID: <9B1A1501A800064397369BD8072E6BCA0652A277@E2K10DB.springfieldclinic.com> Someone told me that you could not use eosin on the new Peloris II. Many of us use eosin to color small biopsies during the process run. We have it in one of the 95% ETOHs. Can someone explain why this can't be used in the Peoris? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From gagnone at KGH.KARI.NET Fri Apr 1 13:08:28 2016 From: gagnone at KGH.KARI.NET (Gagnon, Eric) Date: Fri, 1 Apr 2016 18:08:28 +0000 Subject: [Histonet] bx levels Message-ID: <5F06C3AD0B27264CA20CFA986C87882E019EEF4954@EXCHANGEPV1.KGH.ON.CA> We currently place 3 ribbons of 5-6 sections, at each of three levels, 'down' the slide. This may be too many sections for the pathologists' needs. A suggestion for us going forward would be as Lacy Normington suggests - one or two sections at three or four levels, also, placing these short ribbons 'across' the slide. Currently with long biopsies (over 5 mm) we are unable to fit three ribbons of such longer biopsies on one slide, necessitating the use (and cost, and storage) of a second slide. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From melsmith at udel.edu Fri Apr 1 13:41:26 2016 From: melsmith at udel.edu (Melanie Smith) Date: Fri, 1 Apr 2016 14:41:26 -0400 Subject: [Histonet] Automatic Slide Imager Message-ID: Hello all, I was wondering if anyone would be able to share their per slide costs for utilizing an automatic slide imager for budgeting purposes. We're trying to get an idea of what typical imaging costs are. Thanks, Melanie From rsrichmond at gmail.com Sat Apr 2 12:46:16 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 2 Apr 2016 13:46:16 -0400 Subject: [Histonet] Eosin tissue marker on Peloris II Message-ID: Jim Vickroy, Histology Manager, Springfield Clinic, Springfield, Illinois asks: >>Someone told me that you could not use eosin on the new Peloris II. Many of us use eosin to color small biopsies during the process run. We have it in one of the 95% ETOHs. Can someone explain why this can't be used in the Peoris?<< You shouldn't be coloring small biopsy specimens with eosin on any processor, because eosin's brilliant fluorescence interferes with FISH procedures (such as HER2 on breast cancers). If you want to use a red marking dye, use safranin O. The solution the microbiologists use in Gram stains works quite well, and is probably just across the hallway for you. Bob Richmond Samurai Pathologist Maryville TN From tony.henwood at health.nsw.gov.au Sun Apr 3 19:17:59 2016 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon, 4 Apr 2016 00:17:59 +0000 Subject: [Histonet] Reference for erythrosin-eosin counterstain for H&E Message-ID: <0237449DE79DBC45B686AB82CDCD16FF0148247B@SVDCMBX-MEX008.nswhealth.net> Hi all, I am doing an H&E workshop next month and one of the Hx counterstains I will be including is Erythrosin-eosin (see below). This counterstain is the preferred by some of our major Histopathology departments in Australia. The issue I have is that I cannot find a reference for this variant of the H&E. Anyone have any ideas? Eosin Y (CI 45380) 5g Erythrosin B (CI 45430) 5g Sodium Hydrogen Carbonate 1.25g Magnesium Sulphate 10g Distilled water 500ml Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principle Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From JMacDonald at mtsac.edu Sun Apr 3 21:57:41 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Sun, 3 Apr 2016 19:57:41 -0700 Subject: [Histonet] Reference for erythrosin-eosin counterstain for H&E In-Reply-To: <0237449DE79DBC45B686AB82CDCD16FF0148247B@SVDCMBX-MEX008.nswhealth.net> References: <0237449DE79DBC45B686AB82CDCD16FF0148247B@SVDCMBX-MEX008.nswhealth.net> Message-ID: There is a reference to erythrosin in Lynch's Medical Laboratory Technology, but it is brief. Culling is the author of the histology chapter, so perhaps he has something more in depth. From: "Tony Henwood (SCHN) via Histonet" To: "histonet at lists.utsouthwestern.edu" Date: 04/03/2016 05:20 PM Subject: [Histonet] Reference for erythrosin-eosin counterstain for H&E Hi all, I am doing an H&E workshop next month and one of the Hx counterstains I will be including is Erythrosin-eosin (see below). This counterstain is the preferred by some of our major Histopathology departments in Australia. The issue I have is that I cannot find a reference for this variant of the H&E. Anyone have any ideas? Eosin Y (CI 45380) 5g Erythrosin B (CI 45430) 5g Sodium Hydrogen Carbonate 1.25g Magnesium Sulphate 10g Distilled water 500ml Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principle Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Mon Apr 4 08:30:16 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 4 Apr 2016 13:30:16 +0000 Subject: [Histonet] GI biopsies Message-ID: <48E053DDF6CE074DB6A7414BA05403F806B359@HRHEX02-HOS.holyredeemer.local> We cut 3 levels, 40 microns apart, all mounted on the same slide Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 From Mari.Yang at Hoag.org Mon Apr 4 10:00:25 2016 From: Mari.Yang at Hoag.org (Yang, Mari) Date: Mon, 4 Apr 2016 08:00:25 -0700 Subject: [Histonet] Medical Laboratory Survey Invitation In-Reply-To: <02A9B6BBE70204458C12BF79FEEF018C110500D6@HHEXMB03.hoag.org> References: <02A9B6BBE70204458C12BF79FEEF018C11050033@HHEXMB03.hoag.org> <02A9B6BBE70204458C12BF79FEEF018C110500D6@HHEXMB03.hoag.org> Message-ID: <02A9B6BBE70204458C12BF79FEEF018C1105011A@HHEXMB03.hoag.org> All, Thank you to those who have already responded to the survey invitation. The survey has been extended for one more week. Please feel free to invite any colleagues working in a medical laboratory in the United States to participate. Thank you for taking the time to voice your opinion for our profession. Survey Link: https://www.surveymonkey.com/r/77X9X58 Best regards, Mari Yang, MHA, CT(ASCP)HTL From: Yang, Mari Sent: Thursday, March 24, 2016 5:11 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Medical Laboratory Survey Invitation Dear Histonet, My name is Mari Yang and I am a doctorate student pursuing a degree in Management and Organizational Leadership. I have the unique opportunity to facilitate a study exploring the extent to which spiritual leadership positively influences organizational commitment, productivity, life satisfaction, and negatively influences burnout in the medical laboratory. I invite all persons over the age of 18 who are currently working in a medical laboratory in the US to participate. I am passionate about this study because I believe the findings will add value to the medical laboratory profession. Thank you for taking the time to participate. The public announcement for this study is posted on the ASCP Lab Forum http://bit.ly/224IwVE or you may go directly to the survey https://www.surveymonkey.com/r/77X9X58 Thank you in advance for your contribution. Best regards, Mari Yang, MHA, CT(ASCP)HTL P Save a tree, please don't print this e-mail unless you really need to. Please note that the information contained in this message and any files transmitted with it are privileged and confidential and are protected from disclosure under the law, including the Health Insurance Portability and Accountability Act (HIPAA). If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited and may subject you to criminal or civil penalties. If you have received this communication in error, please notify the sender by replying to the message and delete the material from any computer. Thank you, Hoag Memorial Hospital Presbyterian and its Affiliates From Judith_Pardue at memorial.org Tue Apr 5 04:42:53 2016 From: Judith_Pardue at memorial.org (Pardue, Judith) Date: Tue, 5 Apr 2016 09:42:53 +0000 Subject: [Histonet] IHC Staining Message-ID: Is anyone having a problem with red chromogen verses brown staining on HMB-45. We are having inconsistent staining with the red on HMB-45. Judith Pardue Histology Supervisor CHI Memorial Health Care Chattanooga, Tn. Judith_pardue at memorial.org This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From aeck at dh.org Tue Apr 5 10:01:46 2016 From: aeck at dh.org (Eck, Allison) Date: Tue, 5 Apr 2016 15:01:46 +0000 Subject: [Histonet] expired antibodies Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A972353E1@DH-MAIL01.dhorg.org> Does anyone know of anyone who will accept expired antibodies for research or educational purposes? I appreciate any feedback Thanks Allison Eck, HTL(ASCP)cm, AHI(AMT) Lead Tech Histology Doylestown Hospital 595 W State St Doylestown, PA 18901 215-345-2264 aeck at dh.org From Kimberly at animalreferencepathology.com Tue Apr 5 12:18:52 2016 From: Kimberly at animalreferencepathology.com (Kimberly Marshall) Date: Tue, 5 Apr 2016 17:18:52 +0000 Subject: [Histonet] Used VIP 5 parts Message-ID: Hello fellow Histo folks. I am looking for some advise on purchasing used parts for a VIP 5. Looking for the bottles. Thanks in advance Kimberly Marshall H.T.(ASCP) Histology/Lab Supervisor Toll Free 1-800-426-2099 Fax 801-584-5104 PO Box 17580 Salt Lake City, Utah 84107 www.animalreferencepathology.com Advancing the art and science of veterinary medicine [cid:image001.jpg at 01CF8F87.A0BD4830] From greg.dobbin at gmail.com Tue Apr 5 12:21:09 2016 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Tue, 5 Apr 2016 14:21:09 -0300 Subject: [Histonet] IHC Staining (Pardue, Judith) Message-ID: Judith, The red chromagen is suseptible to "fading" if stained slides are left in either alcohol or xylene for too long. Check to make sure that everyone involved is following the same procedure for the dehydraytion, clearing and mounting of the stained slides. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 med*lab*professionals.ca *Everything in moderation...even moderation itself**!* From b-frederick at northwestern.edu Tue Apr 5 13:12:27 2016 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Tue, 5 Apr 2016 18:12:27 +0000 Subject: [Histonet] Mycoplasma Message-ID: Hello all, Will PAS stain mycoplasma? Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu From LNormington at uwhealth.org Tue Apr 5 13:15:14 2016 From: LNormington at uwhealth.org (Normington Lacy) Date: Tue, 5 Apr 2016 18:15:14 +0000 Subject: [Histonet] Mycoplasma In-Reply-To: References: Message-ID: <7F7174244DDD1A49BFBEE8A845BC2F2B19FB91@UWHC-MBX05.uwhis.hosp.wisc.edu> I do not believe it will stain with PAS. The bacteria lack a cell wall. -----Original Message----- From: Bernice Frederick via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, April 05, 2016 1:12 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Mycoplasma Hello all, Will PAS stain mycoplasma? Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From johnronan at custompathlabsolutions.com Tue Apr 5 13:21:37 2016 From: johnronan at custompathlabsolutions.com (John Ronan) Date: Tue, 5 Apr 2016 14:21:37 -0400 Subject: [Histonet] Used equipment In-Reply-To: References: Message-ID: Hi Histonetters, I worked in an in-office histology lab for 4 years and the lab recently closed. The equipment is in good working condition and is being offered for sale at very good prices. On behalf of Gary Casino, Practice Manager at Somerset Urological Associates in Somerville NJ, I want to let you know that the following equipment is for sale. Please let me know if you you are interested or need pictures or more info. John Ronan Johnronan at custompathlabsolutions.com GROSS LAB JUNIOR$1800 SHANDON WAX DISPENSER$300 SHANDON CONVECTION OVEN$300 22 GAL EAGLE FLAMMABLE STORAGE CABINET$575 in excellent condition 17 GAL JUSTRITE FLAMMABLE STORAGE CABINET$90.00 SAKURA DRS 2000 AUTO SLIDE stainer$5000 Tissuewave 2 microwave processor$5,000 From tejohnson at genoptix.com Tue Apr 5 13:49:37 2016 From: tejohnson at genoptix.com (Teri Johnson) Date: Tue, 5 Apr 2016 18:49:37 +0000 Subject: [Histonet] Validating IHC on decalcified specimens Message-ID: <5970db543ff740309a29e29bf5d8d648@PHUSCB-SP37MB03.genoptix.org> Hi Histonetters, Can someone tell me if they do anything specific to validate their IHC markers on decalcified specimens? If no, do you test them anyway? Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From Richard.Cartun at hhchealth.org Tue Apr 5 14:54:45 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 5 Apr 2016 19:54:45 +0000 Subject: [Histonet] Validating IHC on decalcified specimens In-Reply-To: <5970db543ff740309a29e29bf5d8d648@PHUSCB-SP37MB03.genoptix.org> References: <5970db543ff740309a29e29bf5d8d648@PHUSCB-SP37MB03.genoptix.org> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B0BF3EA@HHCEXCHMB03.hhcsystem.org> I have a validation file for ER, PR, and HER2 IHC testing on decalcified bone specimens with metastatic breast cancer. I compared the results with other specimens from the same patient (usually the primary breast cancer) that were not decalcified. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Teri Johnson via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, April 05, 2016 2:50 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Validating IHC on decalcified specimens Hi Histonetters, Can someone tell me if they do anything specific to validate their IHC markers on decalcified specimens? If no, do you test them anyway? Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From liz at premierlab.com Tue Apr 5 15:02:53 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Tue, 5 Apr 2016 14:02:53 -0600 Subject: [Histonet] Validating IHC on decalcified specimens In-Reply-To: <5970db543ff740309a29e29bf5d8d648@PHUSCB-SP37MB03.genoptix.org> References: <5970db543ff740309a29e29bf5d8d648@PHUSCB-SP37MB03.genoptix.org> Message-ID: <14E2C6176416974295479C64A11CB9AE02BEC2AD5CDB@SBS2K8.premierlab.local> Teri We have a spot on all of our IHC SOP's that will state if the antibody has been tested on decalcified samples. We do not routinely add decalcified tissue to our routine IHC protocol development runs, so for example if the target is a soft tissue target we will develop on a variety of soft tissues that express different staining intensities. If the target is expressed in bone and decalcified bone is the target tissue we will work with both decalcified and soft tissue during the development process. If we are taking a previously soft tissue antibody and testing it in bone or a sample that has been decalcified then that information is added to our internal tracking documents and then the SOP as a revision, we follow the same protocol if we are testing on another species that we have not previously stained for. I can give you an example of the last scenario. We are frequently requested to run GFAP IHC sometimes the samples are routinely fixed on other occasions the skull has been left intact and the sample decaled, we initially developed and verified on soft tissue but later added to the verification that the antibody was tested on decaled samples and we would list the decalcification method/s tested in the SOP. I'll send off examples of both our GLP and non-GLP IHC protocols in a different e-mail. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Teri Johnson via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, April 05, 2016 12:50 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Validating IHC on decalcified specimens Hi Histonetters, Can someone tell me if they do anything specific to validate their IHC markers on decalcified specimens? If no, do you test them anyway? Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mills at 3scan.com Tue Apr 5 16:21:04 2016 From: mills at 3scan.com (Caroline Miller) Date: Tue, 5 Apr 2016 14:21:04 -0700 Subject: [Histonet] Looking for an interesting conversation regarding RNA and Formalin fixation Message-ID: Hi there Histonet, I am looking to have a conversation regarding regular histology processing, mainly formalin fixation, on the RNA species of cell. I know there is a lot of problem in this space and would like to talk to someone who is embedded in this world and could offer advice / services to accurately analyse RNA in formalin fixed, paraffin embedded (and even maybe resin) tissues. Asking a lot - yes, I know! However there are a lot of bright folks out there that might just have an answer for me! Please PM me! yours, mills -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From classicdoc at gmail.com Tue Apr 5 19:36:41 2016 From: classicdoc at gmail.com (Douglas Gregg) Date: Tue, 5 Apr 2016 20:36:41 -0400 Subject: [Histonet] Histonet Digest, Vol 149, Issue 5 In-Reply-To: References: Message-ID: Expired antibodies. Hmm. I got this Himalayan sea salt that says it was created 250 million years ago. Label says the expiration date is 2017. Guess they dug it up just in time. Doug Gregg :-) On Tue, Apr 5, 2016 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. IHC Staining (Pardue, Judith) > 2. expired antibodies (Eck, Allison) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 5 Apr 2016 09:42:53 +0000 > From: "Pardue, Judith" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] IHC Staining > Message-ID: > < > F8593D93099A864A9730359FA6992A879279F038 at CHIEX016.CHI.catholichealth.net> > > Content-Type: text/plain; charset="us-ascii" > > Is anyone having a problem with red chromogen verses brown staining on > HMB-45. We are having inconsistent staining with the red on HMB-45. > > > Judith Pardue > Histology Supervisor > CHI Memorial Health Care > Chattanooga, Tn. > Judith_pardue at memorial.org > > This email and attachments contain information that may be confidential or > privileged. If you are not the intended recipient, notify the sender at > once and delete this message completely from your information system. > Further use, disclosure, or copying of information contained in this email > is not authorized, and any such action should not be construed as a waiver > of privilege or other confidentiality protections. > > > ------------------------------ > > Message: 2 > Date: Tue, 5 Apr 2016 15:01:46 +0000 > From: "Eck, Allison" > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: [Histonet] expired antibodies > Message-ID: > <4ED8C96A8F20FC4F883A92E2A0A0D64A972353E1 at DH-MAIL01.dhorg.org> > Content-Type: text/plain; charset="us-ascii" > > Does anyone know of anyone who will accept expired antibodies for research > or educational purposes? > I appreciate any feedback > Thanks > > Allison Eck, HTL(ASCP)cm, AHI(AMT) > Lead Tech Histology > Doylestown Hospital > 595 W State St > Doylestown, PA 18901 > 215-345-2264 > aeck at dh.org > > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 149, Issue 5 > **************************************** > From j.benavides at eae.csic.es Wed Apr 6 02:52:26 2016 From: j.benavides at eae.csic.es (Julio Benavides) Date: Wed, 6 Apr 2016 09:52:26 +0200 Subject: [Histonet] Mycoplasma In-Reply-To: References: Message-ID: <5704C03A.5070202@eae.csic.es> No, it won?t. You better think on IHC for labelling mycoplasma. cheers Julio El 05/04/2016 a las 20:12, Bernice Frederick via Histonet escribi?: > Hello all, > Will PAS stain mycoplasma? > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick at northwestern.edu > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed Apr 6 09:09:23 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 6 Apr 2016 10:09:23 -0400 Subject: [Histonet] Have you seen Facebook's Million Dollar Interview Question? Message-ID: <012201d1900d$ec2928b0$c47b7a10$@earthlink.net> Hi Histopeeps! I hope you are having a great week! Coming soon to an interview near you Have you seen Facebook?s Million Dollar interview question? Here it is ?On your very best day at work - the day you come home and think you have the best job in the world -what did you do that day?? Facebook uses this question to find out what their candidates are passionate about. I can send you the link to the full article. If you were asked this question how would you answer it? If your answer doesn?t match up with your current position we might need to talk! I can help you find the position that you are passionate about, the place where every day is your best day at work. I have many techs that I have worked with over the years that can answer that question with enthusiasm it deserves because I helped them find that job. Let me help you too. If you are looking for a job today, tomorrow, in 3 months, 6 months, a year, 5 years. I am here for you. My best day at work is every day since my passion is to find the right opportunity for you! Here are the areas where my current open positions are located. Norfolk, VA Louisville, KY Los Angeles, CA Kansas City, KS Fayetteville, AR Milwaukee, WI Great Falls, MT Flagstaff, AZ Austin, TX Tyler, TX Chattanooga, TN All of these opportunities are full time permanent positions with some of the leading employers nationwide. My clients offer excellent compensation, benefits and relocation/sign on bonuses. Please contact me at relia1 at earthlink.net or toll free at 866-607-3542 or call/text me on my cell at 407-353-5070 if you are interested in more information on any of these positions or if you would like for me to work on a custom job search for you. Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From LNormington at uwhealth.org Wed Apr 6 09:38:46 2016 From: LNormington at uwhealth.org (Normington Lacy) Date: Wed, 6 Apr 2016 14:38:46 +0000 Subject: [Histonet] IgG Subtyping on FFPE Message-ID: <7F7174244DDD1A49BFBEE8A845BC2F2B1A20EA@UWHC-MBX05.uwhis.hosp.wisc.edu> I am looking for information on where to purchase IgG-1, IgG-2, IgG-3 and IgG-4 antibodies to be used on formalin fixed paraffin embedded tissue. I would prefer that this be an IHC antibody instead of FITC. Does anyone have experience with IgG subtyping? If so, what do you do? Thanks Lacy Normington Lacy Normington, HTL(ASCP)CM Manager, Surgical Pathology Lab Services UWHospital and Clinics 600 Highland Avenue Madison, WI 53792-2472 Phone: 608-890-9373 From Linda.Margraf at cookchildrens.org Wed Apr 6 11:46:24 2016 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Wed, 6 Apr 2016 16:46:24 +0000 Subject: [Histonet] specimen submission pads Message-ID: <7898c70581034cd7b8f31a1827b78f73@MBX10.CCHCS.LDAP> Hi Histonetters, We are having trouble in the Gross room, removing small specimens from the pads/gauze that OR personnel put them on which they then immerse in formalin. We thought the gauze was challenging to remove tiny specimens from but have found that the Telfa pads the OR is now using come apart in the fixative and are even more challenging to work with. The GI lab uses sponges but they would be too small for the usual size containers the OR send us. Any suggestions? Thanks in advance, Linda M From wbenton at cua.md Wed Apr 6 12:00:22 2016 From: wbenton at cua.md (Walter Benton) Date: Wed, 6 Apr 2016 17:00:22 +0000 Subject: [Histonet] specimen submission pads In-Reply-To: <7898c70581034cd7b8f31a1827b78f73@MBX10.CCHCS.LDAP> References: <7898c70581034cd7b8f31a1827b78f73@MBX10.CCHCS.LDAP> Message-ID: <3896cd5e620f4c3584564251d205cf66@MAIL01.GCU-MD.local> Biopsy wraps or http://www.amazon.com/Graham-Jumbo-Papers-Perms-1000ct/dp/B001J9R93W Either of these items can be wrapped to form an envelope preventing the tissue from floating around. -----Original Message----- From: Linda Margraf via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 06, 2016 12:46 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] specimen submission pads Hi Histonetters, We are having trouble in the Gross room, removing small specimens from the pads/gauze that OR personnel put them on which they then immerse in formalin. We thought the gauze was challenging to remove tiny specimens from but have found that the Telfa pads the OR is now using come apart in the fixative and are even more challenging to work with. The GI lab uses sponges but they would be too small for the usual size containers the OR send us. Any suggestions? Thanks in advance, Linda M _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From aeck at dh.org Wed Apr 6 13:10:44 2016 From: aeck at dh.org (Eck, Allison) Date: Wed, 6 Apr 2016 18:10:44 +0000 Subject: [Histonet] expired antibodies In-Reply-To: <4ED8C96A8F20FC4F883A92E2A0A0D64A972376E5@DH-MAIL01.dhorg.org> References: <4ED8C96A8F20FC4F883A92E2A0A0D64A972353E1@DH-MAIL01.dhorg.org> <4ED8C96A8F20FC4F883A92E2A0A0D64A972376E5@DH-MAIL01.dhorg.org> Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A972376F0@DH-MAIL01.dhorg.org> Thank you to everyone who responded. I had no idea there are so many of you that can use them. -----Original Message----- From: Eck, Allison via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, April 05, 2016 11:02 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] expired antibodies Does anyone know of anyone who will accept expired antibodies for research or educational purposes? I appreciate any feedback Thanks Allison Eck, HTL(ASCP)cm, AHI(AMT) Lead Tech Histology Doylestown Hospital 595 W State St Doylestown, PA 18901 215-345-2264 aeck at dh.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis at bresnan.net Wed Apr 6 14:58:13 2016 From: gayle.callis at bresnan.net (Gayle Callis) Date: Wed, 6 Apr 2016 13:58:13 -0600 Subject: [Histonet] Journal of Histotechnology special issue call for manuscripts Message-ID: <001701d1903e$a79161f0$f6b425d0$@bresnan.net> Dear Histonetters, The Journal of Histotechnology is calling for manuscripts to be published in December, 2016 . The manuscript submission deadline is no later than July 1st to allow for a review process. Any late submissions would be published in a future issue. This is a special issue on troubleshooting routine hematoxylin and eosin and other special stains on paraffin embedded tissue sections. Modes of staining can be either automated or manual. With advanced staining technology, there is particular interest in problem solving for automated staining. Immunohistochemical troubleshooting is not included in this issue. The manuscript can be a Rapid Research Communication, a longer, more comprehensive document or a scholarly review. To submit a manuscript, go to: http://www.editorialmanager.com/his/ then click on button to "register now". There also a link to "Instructions for Authors" on that cover page. If help is needed with manuscript writing, the JOH Writing Partners Program is available to guide author(s) through the scientific writing process. If there are any questions, you can contact the journal via the NSH.org website. The email contact for JOH is found under publications. You do not have to be an NSH member to submit manuscripts to this journal. Thank you Gayle M. Callis HTL/HT/MT(ASCP) Acting Editor for Troubleshooting Staining Special Issue Journal of Histotechnology National Society for Histotechnology From careerstudio at bellsouth.net Wed Apr 6 15:42:59 2016 From: careerstudio at bellsouth.net (Barbara Siegel) Date: Wed, 6 Apr 2016 16:42:59 -0400 Subject: [Histonet] Histology Technologist Position / shift differential / south Georgia Message-ID: <00ae01d19044$e9389370$bba9ba50$@net> We have a fine pathology services client - full service histo lab using Ventana IHC seeking a Histology Technologist for Shift: 10 pm to 6 am or 11 p.m to 7 am. There is a shift differential. Relocation assistance is available to this desirable geography located 1 hour south of Savannah Georgia This position requires individuals who are able to handle a large volume of work, ability to take direction, & a commitment to consistently create quality solutions. Will performs routine & non-routine activities involved in the preparation of slides, for microscopic evaluation by pathologist(s) Accountabilities: ? Ensure proper accessioning & labeling of all tissue samples. ? Process paperwork associated with accessioning & reporting. ? Ensure proper tissue processing. ? Embed processed tissue in paraffin. ? Perform microtomy of embedded tissue ? Prepare slides for routine Hematoxylin & Eosin staining. ? Perform coverslipping of stained slides either manually or automated ? Prepare solutions & reagents for special stain procedures. ? Perform special stain procedures. ? Perform filing of finished blocks & slides. ? Perform routine maintenance & cleaning of equipment & troubleshoot minor equipment failures. ? Document remedial actions such as repairs & repeated tests. ? Adhere to laboratory?s quality control policies, & document all quality control activities. ? Ensure all corporate safety, quality control & quality assurance standards are met. ? Ensure compliance with all local, federal, CLIA & CAP regulations Requirements: Associates degree & 1+ years of full time exp OR H.S. education or equivalent & 5 years. HT (ASCP) or ASCP eligible OR five years of full time experience Our search has one of the finest biotechnology divisions, focused on the clinical laboratory sector. Please contact David King at biolabcareers at aol.com for more information. Career Studio national search 561-738-6363 ? Visit us on linkedin: http://www.linkedin.com/in/biotechnologyhires From yesyes at comcast.net Wed Apr 6 16:02:38 2016 From: yesyes at comcast.net (yesyes at comcast.net) Date: Wed, 6 Apr 2016 21:02:38 +0000 (UTC) Subject: [Histonet] Histology position florida Message-ID: <282863184.4696318.1459976558276.JavaMail.zimbra@comcast.net> From yesyes at comcast.net Wed Apr 6 16:06:46 2016 From: yesyes at comcast.net (yesyes at comcast.net) Date: Wed, 6 Apr 2016 21:06:46 +0000 (UTC) Subject: [Histonet] Histology position florida Message-ID: <686507719.4698934.1459976806526.JavaMail.zimbra@comcast.net> From yesyes at comcast.net Wed Apr 6 16:33:14 2016 From: yesyes at comcast.net (yesyes at comcast.net) Date: Wed, 6 Apr 2016 21:33:14 +0000 (UTC) Subject: [Histonet] Histology position Florida Message-ID: <620151033.4712495.1459978394113.JavaMail.zimbra@comcast.net> From yesyes at comcast.net Wed Apr 6 16:33:20 2016 From: yesyes at comcast.net (yesyes at comcast.net) Date: Wed, 6 Apr 2016 21:33:20 +0000 (UTC) Subject: [Histonet] Histology position Florida Message-ID: <6897021.4712520.1459978400710.JavaMail.zimbra@comcast.net> From criley at dpspa.com Thu Apr 7 04:43:45 2016 From: criley at dpspa.com (Charles Riley) Date: Thu, 7 Apr 2016 05:43:45 -0400 Subject: [Histonet] Reprocessing Message-ID: Hello all, We had an issue with our processor the other night. Someone accidentally put 100% alochol into the last xylene station. The tissue sections were difficult to cut and or were unreadable. What is the best way to reprocess them? They are mostly small GI biopsies and only two larger specimens -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From LRaff at uropartners.com Thu Apr 7 08:45:48 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 7 Apr 2016 13:45:48 +0000 Subject: [Histonet] Blog Post--not work related Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B7D252C@COLOEXCH01.uropartners.local> http://www.chicagonow.com/downsize-maybe/2016/04/its-a-puzzlement-putting-the-pieces-together-when-building-a-house/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From clmcmah at clemson.edu Thu Apr 7 10:01:34 2016 From: clmcmah at clemson.edu (Chad L McMahan) Date: Thu, 7 Apr 2016 15:01:34 +0000 Subject: [Histonet] Embedding Molds Message-ID: Hello all Histonetters: Our labs are in need of deep metal embedding molds (dimensions 32X25X12 mm). They are the metal Sakura Tissue Tek brand. If you have a stash setting around getting dusty then we will gladly put them to great use! Let me know if you have some that you would love to part with! Thanks, Chad M. Chad L. McMahan, MBA, HT (ASCP)cm Clemson Bioengineering Biomaterials/Histology Lab Manager BioE Research Safety Liaison clmcmah at clemson.edu From mwerdler at gmail.com Thu Apr 7 10:02:25 2016 From: mwerdler at gmail.com (Mca Werdler) Date: Thu, 7 Apr 2016 10:02:25 -0500 Subject: [Histonet] Reprocessing In-Reply-To: References: Message-ID: What i would do, melt the blocks you made. 1. Put them back in paraffin for one hour 2. Put them back in another paraffin or one hour 3. Put them in xylene for one hour 4. Put them in another xylene for one hour 5. Put them in another xylene for one hour 6. Put them back in paraffin for one hour 7. Put them back in paraffin for one hour. It takes some time. The reason why it was dificult to cut is because the alcohol and paraffin dont mix, there is a possibility that the parafin was not well enough impregnated in the tissue. With the above solution, you can restore that. Maarten UNAM neurobiologia, histolog?a Mexico 2016-04-07 4:43 GMT-05:00 Charles Riley via Histonet < histonet at lists.utsouthwestern.edu>: > Hello all, > > We had an issue with our processor the other night. Someone accidentally > put 100% alochol into the last xylene station. The tissue sections were > difficult to cut and or were unreadable. What is the best way to reprocess > them? They are mostly small GI biopsies and only two larger specimens > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ashnews at comcast.net Thu Apr 7 10:04:00 2016 From: ashnews at comcast.net (ashnews at comcast.net) Date: Thu, 7 Apr 2016 15:04:00 +0000 (UTC) Subject: [Histonet] Missouri/Arkansas Joint Spring Meeting in Branson MO May 19th-20th In-Reply-To: <1990039250.6012429.1460041138878.JavaMail.zimbra@comcast.net> Message-ID: <939160553.6018102.1460041440015.JavaMail.zimbra@comcast.net> Want something interesting to do May 19-21, 2016? Attend the Missouri/Arkansas Histotechnology Annual Symposium in Branson, MO It's a beautiful time of year to see Branson! * ?Earn 13 CEU's to apply to your ASCP CMP Program! * ?Browse the 16 exhibitors, network & see all the new products. * ?Attend the HT Readiness Workshop all day Saturday and be entered in a drawing for? a $500.00 Educational Scholarship sponsored by StatLab! * ?Visit the NSH Booth see what's new and be entered into a drawing for a free membership NSH! * Stop in and enjoy Ada Feldman's "The Joy of Cooking" lecture on Thursday evening and then on to the Vendor Reception! * Enjoy a complimentary luncheon/presentation on Friday presented by Myriad Genetics. We would love to see you! Deadline for Radisson Hotel Branson is? April 19, 2016. Make your reservations early! If you are coming but are awaiting funds just return your registration form so we know your coming! For more information or a program Contact Pam Marcum ashnews at comcast.net or Sharon Walsh userwalsh at sbcglobal.net From ashnews at comcast.net Thu Apr 7 10:05:42 2016 From: ashnews at comcast.net (ashnews at comcast.net) Date: Thu, 7 Apr 2016 15:05:42 +0000 (UTC) Subject: [Histonet] Missouri/Arkansas Spring Meeting In Branson MO - Join Us!! In-Reply-To: <2033010560.6018750.1460041484557.JavaMail.zimbra@comcast.net> Message-ID: <12268878.6019494.1460041542213.JavaMail.zimbra@comcast.net> Want something interesting to do May 19-21, 2016? Attend the Missouri/Arkansas Histotechnology Annual Symposium in Branson, MO It's a beautiful time of year to see Branson! * ?Earn 13 CEU's to apply to your ASCP CMP Program! * ?Browse the 16 exhibitors, network & see all the new products. * ?Attend the HT Readiness Workshop all day Saturday and be entered in a drawing for? a $500.00 Educational Scholarship sponsored by StatLab! * ?Visit the NSH Booth see what's new and be entered into a drawing for a free membership NSH! * Stop in and enjoy Ada Feldman's "The Joy of Cooking" lecture on Thursday evening and then on to the Vendor Reception! * Enjoy a complimentary luncheon/presentation on Friday presented by Myriad Genetics. We would love to see you! Deadline for Radisson Hotel Branson is? April 19, 2016. Make your reservations early! If you are coming but are awaiting funds just return your registration form so we know your coming! For more information or a program Contact Pam Marcum ashnews at comcast.net or Sharon Walsh userwalsh at sbcglobal.net From gayle.callis at bresnan.net Thu Apr 7 11:06:16 2016 From: gayle.callis at bresnan.net (Gayle Callis) Date: Thu, 7 Apr 2016 10:06:16 -0600 Subject: [Histonet] Reprocessing In-Reply-To: References: Message-ID: <000001d190e7$6ad3de10$407b9a30$@bresnan.net> A quick technique, published in Histologic Vol XXXVI, No. 1, May 2003 http://www.sakura-americas.com/Histologic/Histo-Tips/1008.htmlby Michael Johnson, A technic for correcting poorly processed paraffin blocks. Melt the paraffin, blot to remove excess melted paraffin, place in cassette. Put on processor with rest of tissues sitting in NBF for daily processing run. This way you don't have to go through the agony of trying going back and then forward through reagents and the tissues will be less damaged by more heating and exposure to drying reagents. Good luck Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: Mca Werdler via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, April 07, 2016 9:02 AM To: Charles Riley Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Reprocessing What i would do, melt the blocks you made. 1. Put them back in paraffin for one hour 2. Put them back in another paraffin or one hour 3. Put them in xylene for one hour 4. Put them in another xylene for one hour 5. Put them in another xylene for one hour 6. Put them back in paraffin for one hour 7. Put them back in paraffin for one hour. It takes some time. The reason why it was dificult to cut is because the alcohol and paraffin dont mix, there is a possibility that the parafin was not well enough impregnated in the tissue. With the above solution, you can restore that. Maarten UNAM neurobiologia, histolog?a Mexico 2016-04-07 4:43 GMT-05:00 Charles Riley via Histonet < histonet at lists.utsouthwestern.edu>: > Hello all, > > We had an issue with our processor the other night. Someone > accidentally put 100% alochol into the last xylene station. The tissue > sections were difficult to cut and or were unreadable. What is the > best way to reprocess them? They are mostly small GI biopsies and > only two larger specimens > > -- > > Charles Riley HT(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From careerstudio at bellsouth.net Thu Apr 7 13:47:38 2016 From: careerstudio at bellsouth.net (Barbara Siegel) Date: Thu, 7 Apr 2016 14:47:38 -0400 Subject: [Histonet] Day Shift Lab / Histology Supervisor opportunity in St Louis, MO Message-ID: <003f01d190fd$f69ee870$e3dcb950$@net> Our foremost clinical laboratory client is seeking a (Day Shift) Lab & Patient Services Management Supervisor to be responsible for the day-to-day operations of the Histology laboratory & supervision of the technical & support staff, based in St. Louis, Missouri. In conjunction with the Department Manager, this individual will ensure that all departmental policies & procedures meet the standards of current state & federal regulations as follows: * Coordinate Histotechnologists, Lab Aides, Grossing Personnel, & related staff, Schedule & maintain adequate coverage & effective utilization of personnel. * Monitor & control expenses in line with department budget; Assist in the preparation of the budget & recommend capital equipment needs. * Maintain appropriate levels of supplies & reagents commensurate with workload; Maintain equipment & instruments in good operating condition, recognize any malfunctions & troubleshoot. * Provide technical instructions & training of personnel in techniques, instrumentation, & organization of work; Maintain a current procedure manual, reviewing & revising as needed. * Interview & select potential employees in conjunction with the Manager; \Write & conduct employee performance evaluations; Ensure that all employees follow all company & department policies; initiate performance improvement with employees. * Maintain attendance records; approve overtime & vacation time; Ensure proper time record editing; Maintain appropriate Quality Assurance documentation. * Provide backup for bench Histotechnologists in sectioning & staining. Participate in company meetings & conduct departmental meetings, Maintain good communications with the Histopathology Technical Supervisor & Senior Medical Director/Laboratory Director * Ensure technical quality, so that the Pathologists can adequately evaluate prepared slides & that all outstanding cases are reconciled. * Ensure that all employees comply with all safety regulations, including personal protective equipment; Complete successfully all personnel departmental training & competency assessments; Facilitate & develop continuing education. * Ensure all corporate safety, quality control & quality assurance standards are met, as well as compliance with all local, federal, CLIA & CAP regulations * Interact with medical transcription staff; Engage in client contact, as required; Perform special assigned projects JOB REQUIREMENTS: * BS degree in Biological/Physical sciences or equivalent combination of education & experience. * 3-6 years exp as a Histotechnologist, with 1+ years in a successful leadership role * Meet all qualifications for Histology Laboratory Supervisor SOP. HT (ASCP) or HTL (ASCP) certified. This opportunity offers competitive starting salary, annual incentive plan bonus, attractive benefits package & relocation assistance. Please contact David King at biolabcareers at aol.com Career Studio, Biotech division Barbara Siegel Career Studio careerstudio at bellsouth.net 561-738-6363 www.linkedin.com/in/careerstudio/ From Maxim_71 at mail.ru Thu Apr 7 14:40:21 2016 From: Maxim_71 at mail.ru (Maxim Peshkov) Date: Thu, 7 Apr 2016 22:40:21 +0300 Subject: [Histonet] Reprocessing Message-ID: <1138977490.20160407224021@mail.ru> Here is very good free e-book: http://www.leicabiosystems.com/fileadmin/biosystems/PDF/95.9890_Rev_C_Difficult_Blocks_and_Reprocessing.pdf -- Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71 at mail.ru From SteveM at mcclainlab.com Thu Apr 7 16:05:39 2016 From: SteveM at mcclainlab.com (Steve McClain) Date: Thu, 7 Apr 2016 21:05:39 +0000 Subject: [Histonet] Histonet Digest, Vol 149, Issue 7 : Re: specimen submission pads ordinary lens paper In-Reply-To: References: Message-ID: <5B76E735-6B2F-43A7-98BD-4F8B5276C7E0@mcclainlab.com> There is much commendable about ordinary lens paper cut from 9x12 sheets to ~2x2 inch and folded 4 times like an origami to enclose small tissue fragments. Keeps floaters out. Translucent so you can count the fragments before and after opening. Unfolds reliably at embedding wo flinging pieces. Cleans your forceps after embedding. Doesn't seem to clog processors. 2 hour processing in regular cassettes. We wrap nearly every specimen. I can send a brief video demonstration if you email me directly. Steve A. McClain, MD > On Apr 7, 2016, at 13:26, "histonet-request at lists.utsouthwestern.edu" wrote: > > bject: Re: [Histonet] specimen submission pads From Kerry.Broom at phe.gov.uk Fri Apr 8 09:57:29 2016 From: Kerry.Broom at phe.gov.uk (Kerry Broom) Date: Fri, 8 Apr 2016 14:57:29 +0000 Subject: [Histonet] Bubbling effect under brain tissue sections Message-ID: <1F421268D349034894BA1C8BBE9DE640F8980657@MAILMBXCOL04.phe.gov.uk> I am having problems with our immunohistochemistry on the dako autostainer - please see attached image of a brain section using synaptophysin antibody. But, I don't think the problem is the immunohistochemistry, I think it is happening before we get to that stage. We are using 5% APES coated slides and the sections go through a 95deg heat pretreatment at high pH. We are getting this bubbling effect under the tissue. It looks to be worse on one side of the brain (the top of the slide) Does anyone have any idea what might be causing this? Any suggestions welcome. http://histosearch.com/imageupload/bubbling-effect-under-brain-tissue-sections/ ************************************************************************** The information contained in the EMail and any attachments is confidential and intended solely and for the attention and use of the named addressee(s). It may not be disclosed to any other person without the express authority of Public Health England, or the intended recipient, or both. If you are not the intended recipient, you must not disclose, copy, distribute or retain this message or any part of it. This footnote also confirms that this EMail has been swept for computer viruses by Symantec.Cloud, but please re-sweep any attachments before opening or saving. http://www.gov.uk/PHE ************************************************************************** From abadesuyi at nrh-ok.com Fri Apr 8 13:20:47 2016 From: abadesuyi at nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Fri, 8 Apr 2016 13:20:47 -0500 Subject: [Histonet] Gross Room Message-ID: <04EE4F75BB5FB246ADB68D69B7460443A5177B106C@MAIL.nrhnt.nrh-ok.com> Hi Guys, How are you guys doing? I hope you are all doing great. Please I have a question and my question has to do with the staffing of the Gross Room area. Do you guys put the Lab Assistants or Techs in the Gross Room? Thanks, Adesupo ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From PAMarcum at uams.edu Fri Apr 8 13:47:56 2016 From: PAMarcum at uams.edu (Marcum, Pamela A) Date: Fri, 8 Apr 2016 18:47:56 +0000 Subject: [Histonet] Question About A Sponge Type Message-ID: <5d5e30f47f594197ae7daa90389c458d@MAIL13M2N2.ad.uams.edu> When I was at NSH in Austin, I talked to a vendor with a very different type of Histology. It was very soft and far less porous than the normal blue sponges. Does anyone remember this or can you tell me where to get them? I believe the company was from the UK with offices or distributors in the US. I need to either buy or get some for a demo here with our pathologists. We cannot use the blue sponges for many of our cases as they just too rough and the surfaces are too rough. Thank You, Pam Marcum UAMS ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From craigak12 at gmail.com Fri Apr 8 13:56:53 2016 From: craigak12 at gmail.com (J B) Date: Fri, 08 Apr 2016 18:56:53 +0000 Subject: [Histonet] Gross Room In-Reply-To: <04EE4F75BB5FB246ADB68D69B7460443A5177B106C@MAIL.nrhnt.nrh-ok.com> References: <04EE4F75BB5FB246ADB68D69B7460443A5177B106C@MAIL.nrhnt.nrh-ok.com> Message-ID: We use lab Assistants, and a PA. It has been working great. On Fri, Apr 8, 2016, 11:34 AM Adesupo, Adesuyi (Banjo) via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Guys, > How are you guys doing? I hope you are all doing great. > Please I have a question and my question has to do with the staffing of the > Gross Room area. Do you guys put the Lab Assistants or Techs in the Gross > Room? > > Thanks, > Adesupo > > > ====================================== > CONFIDENTIALITY NOTICE: > > This e-mail communication and any attachments may > contain confidential and privileged information for the use > of the designated recipients named above. If you are not > the intended recipient, you are hereby notified that you > have received this communication in error and that any > review, disclosure, dissemination, distribution, or copying > of it or its contents is prohibited. If you have received > this communication in error, please notify the sender > immediately and destroy all copies of this communication > and any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bakevictoria at gmail.com Fri Apr 8 15:26:37 2016 From: bakevictoria at gmail.com (Victoria Baker) Date: Fri, 8 Apr 2016 16:26:37 -0400 Subject: [Histonet] Cell Blocks Message-ID: Hi - Happy Friday! Is anyone on makeing cellblocks with thromboplasting/plasma on cells fixed in sodium citrate? These would be pleural fluids and bronch brushings and washings. Thanks in advance. Vikki From bhartologist at gmail.com Sat Apr 9 12:41:31 2016 From: bhartologist at gmail.com (Bharti Parihar) Date: Sat, 9 Apr 2016 10:41:31 -0700 Subject: [Histonet] Paraplast In-Reply-To: References: Message-ID: Hello all! I'm looking for feedback for any labs that use paraplast paraffin in their processing and embedding. If any labs use it in the San Francisco Bay area I'd especially appreciate feedback from them to gain comparable data being that the lab I work for is in the bay area also. A specific question I have is: If eosin is being used at your lab in processing with paraplast, what is the volume of eosin being added? To give a sense of the volume of our processors, our block rack for processing holds about 132 blocks/rack, which maybe that's the standard for racks. We can load up to 3 racks in our Peloris processors and 2 racks in our VIP processors. Any feedback on this is greatly appreciated! From choose4health at yahoo.com Sat Apr 9 23:30:07 2016 From: choose4health at yahoo.com (Nan Gray) Date: Sat, 9 Apr 2016 21:30:07 -0700 Subject: [Histonet] Histonet Digest, Vol 149, Issue 9 In-Reply-To: References: Message-ID: <7E8B4EA2-B8DA-4553-AF15-57DA109D53B7@yahoo.com> Remove me from yiur list! Sent from my iPhone > On Apr 9, 2016, at 10:00 AM, histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Gross Room (Adesupo, Adesuyi (Banjo)) > 2. Question About A Sponge Type (Marcum, Pamela A) > 3. Re: Gross Room (J B) > 4. Cell Blocks (Victoria Baker) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 8 Apr 2016 13:20:47 -0500 > From: "Adesupo, Adesuyi (Banjo)" > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: [Histonet] Gross Room > Message-ID: > <04EE4F75BB5FB246ADB68D69B7460443A5177B106C at MAIL.nrhnt.nrh-ok.com> > Content-Type: text/plain; charset="us-ascii" > > Hi Guys, > How are you guys doing? I hope you are all doing great. Please I have a question and my question has to do with the staffing of the Gross Room area. Do you guys put the Lab Assistants or Techs in the Gross Room? > > Thanks, > Adesupo > > > ====================================== > CONFIDENTIALITY NOTICE: > > This e-mail communication and any attachments may > contain confidential and privileged information for the use > of the designated recipients named above. If you are not > the intended recipient, you are hereby notified that you > have received this communication in error and that any > review, disclosure, dissemination, distribution, or copying > of it or its contents is prohibited. If you have received > this communication in error, please notify the sender > immediately and destroy all copies of this communication > and any attachments. > > > ------------------------------ > > Message: 2 > Date: Fri, 8 Apr 2016 18:47:56 +0000 > From: "Marcum, Pamela A" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Question About A Sponge Type > Message-ID: <5d5e30f47f594197ae7daa90389c458d at MAIL13M2N2.ad.uams.edu> > Content-Type: text/plain; charset="us-ascii" > > When I was at NSH in Austin, I talked to a vendor with a very different type of Histology. It was very soft and far less porous than the normal blue sponges. Does anyone remember this or can you tell me where to get them? I believe the company was from the UK with offices or distributors in the US. I need to either buy or get some for a demo here with our pathologists. We cannot use the blue sponges for many of our cases as they just too rough and the surfaces are too rough. > > Thank You, > > Pam Marcum > UAMS > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > > ------------------------------ > > Message: 3 > Date: Fri, 08 Apr 2016 18:56:53 +0000 > From: J B > To: "Adesupo, Adesuyi (Banjo)" , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Gross Room > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > We use lab Assistants, and a PA. It has been working great. > > On Fri, Apr 8, 2016, 11:34 AM Adesupo, Adesuyi (Banjo) via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Hi Guys, >> How are you guys doing? I hope you are all doing great. >> Please I have a question and my question has to do with the staffing of the >> Gross Room area. Do you guys put the Lab Assistants or Techs in the Gross >> Room? >> >> Thanks, >> Adesupo >> >> >> ====================================== >> CONFIDENTIALITY NOTICE: >> >> This e-mail communication and any attachments may >> contain confidential and privileged information for the use >> of the designated recipients named above. If you are not >> the intended recipient, you are hereby notified that you >> have received this communication in error and that any >> review, disclosure, dissemination, distribution, or copying >> of it or its contents is prohibited. If you have received >> this communication in error, please notify the sender >> immediately and destroy all copies of this communication >> and any attachments. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 4 > Date: Fri, 8 Apr 2016 16:26:37 -0400 > From: Victoria Baker > To: Histo Net list server > Subject: [Histonet] Cell Blocks > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Hi - Happy Friday! > > Is anyone on makeing cellblocks with thromboplasting/plasma on cells fixed > in sodium citrate? These would be pleural fluids and bronch brushings and > washings. > > Thanks in advance. > > Vikki > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 149, Issue 9 > **************************************** From PKRichar at gundersenhealth.org Mon Apr 11 07:50:49 2016 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Mon, 11 Apr 2016 12:50:49 +0000 Subject: [Histonet] Microtome workstations Message-ID: <998284C32F61104CA0BEFFFFCF6F90FD6D0A244D@LXEXMB01.gundluth.org> Hi, we are looking for individual microtome workstations. What are you using for your microtome workstations? Are they adjustable? Cordially, Pam ~ +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org From LRaff at uropartners.com Mon Apr 11 08:07:58 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 11 Apr 2016 13:07:58 +0000 Subject: [Histonet] Microtome response and unrelated blog psot Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10CF559A@COLOEXCH01.uropartners.local> We are very satisfied with Leica microtomes and will probably be getting an additional one soon. Yes, they are adjustable. Unrelated blog post: http://www.chicagonow.com/downsize-maybe/2016/04/whats-in-your-wallet-theres-a-little-aluminum-foil-package-in-mine/ Please note, if you cut and paste, grab that last "/" Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From LaurenHegnerSweeney at uga.edu Mon Apr 11 09:42:27 2016 From: LaurenHegnerSweeney at uga.edu (Lauren Marie Hegner) Date: Mon, 11 Apr 2016 14:42:27 +0000 Subject: [Histonet] H&E automated stainers Message-ID: Hello all, Our lab is looking for a new automated H&E stainer and I was wondering if anyone out there has had any experience using Ventana's Symphony System, Hacker's HistoPro 3030, or Leica ST5010 Autostainer XL? OR If anyone has any suggestions as to the best one out there? Thanks, L From rjbuesa at yahoo.com Mon Apr 11 10:08:52 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 11 Apr 2016 15:08:52 +0000 (UTC) Subject: [Histonet] H&E automated stainers In-Reply-To: References: Message-ID: <238058776.642754.1460387332530.JavaMail.yahoo@mail.yahoo.com> Before deciding, ask for a "demo" from Sakura.Ren? On Monday, April 11, 2016 11:02 AM, Lauren Marie Hegner via Histonet wrote: Hello all, Our lab is looking for a new automated H&E stainer and I was wondering if anyone out there has had any experience using Ventana's Symphony System, Hacker's HistoPro 3030, or Leica ST5010 Autostainer XL? OR If anyone has any suggestions as to the best one out there? Thanks, L _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague at Pathologyarts.com Mon Apr 11 15:39:00 2016 From: c.tague at Pathologyarts.com (Curt) Date: Mon, 11 Apr 2016 20:39:00 +0000 Subject: [Histonet] hello histonetters, looking for a little control tissue help Message-ID: <9C8F910F72893643B3C3793C3D67132B6797CAB4@PATHOLOGYSERVER.pathologyarts.local> We are looking for some control tissue that we're having trouble finding. Would like to see if anyone is interested or able to barter some tissue... Now this is going to be the fun part... they're all the hard ones to find.. AML EBV Hairy Cell Leukemia Mantle Cell Lymphoma Mesothelioma Thymus It's never easy asking for help, inconveniencing other people, hate to ask but we just can't find any from our local sources. Best regards and thanks for any help, Curt From Linda.Margraf at cookchildrens.org Mon Apr 11 17:04:48 2016 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Mon, 11 Apr 2016 22:04:48 +0000 Subject: [Histonet] Stain problem Message-ID: <41de30502bc845a9b3cac4d371445ef2@MBX10.CCHCS.LDAP> (Here is a message I am posting for Natacha....please reply to the list so she sees it. Thanks Linda M, Histonet administrator) Hi histonetters, Lately we have had a special stain phenomenon occurring with our recent liver biopsies on our NexES special stainer. The nuclear staining is obsolete. Where the nucleus should be there are empty spaces. The liver control sections are staining fine with proper digestion and nuclear staining but the patient sections are show no nuclear staining. We have seen this in the past and describe it as a "nuclear bubbling" affect with the PAS/D on this stainer. However, the current nuclear staining is an extreme case. Any thoughts. Our protocol is Schiff's 4 min @ 60?C and diastase for 12 min. Natacha M. Houston, Tx From BZIMMERM at gru.edu Tue Apr 12 10:26:29 2016 From: BZIMMERM at gru.edu (Zimmerman, Billie) Date: Tue, 12 Apr 2016 15:26:29 +0000 Subject: [Histonet] HISTOPALOOZA FAST APPROACHING APRIL 22ND - APRIL 24TH Message-ID: If you're planning on joining us at Legacy Lodge Lake Lanier Georgia, please secure your room ASAP. Remember that admittance to the lodge is free with your room reservation. There will be valet as well as self-parking at the Lodge. Time is running out for all you procrastinators!! Hope to see all of you there. Address is 7000 Lanier Islands Parkway, Buford, GA 30518 (GPS works with this address, tested by the gal who always ends up either on a dead end or a cow pasture) Billie Zimmerman GSH Secretary From doolee at shands.ufl.edu Tue Apr 12 12:42:59 2016 From: doolee at shands.ufl.edu (Dooley, Elaine) Date: Tue, 12 Apr 2016 17:42:59 +0000 Subject: [Histonet] adenovirus control block Message-ID: <6193C53146742E4586DD4838F47F79AE0EA3F9CB@MSXMB02.Shands.local> Hi Histonetters, Would someone out there have an adenovirus paraffin control block that they could spare. I could trade some parvovirus controls or something else for an adenovirus control block. Elaine Dooley Shands labs at Rocky Point 4800 SW 35th Drive Gainesville FL 32608 352-265-0111 ext 72117 From ihcworkshop at gmail.com Tue Apr 12 13:18:25 2016 From: ihcworkshop at gmail.com (Ihc Workshop) Date: Tue, 12 Apr 2016 11:18:25 -0700 Subject: [Histonet] IHC Training Course 12 CEU, Message-ID: <52B51F78-0D5D-4612-96CE-CC3A4DCC9781@gmail.com> Hands-on IHC Training course in manual staining of human and animal tissues, includes troubleshooting session, June 23 & 24, 2016. San Francisco Bay Area, space is limited. For details contact Maria ihcworkshop at gmail.com From Victoria_Verno at bshsi.org Tue Apr 12 13:40:38 2016 From: Victoria_Verno at bshsi.org (Verno, Victoria R.) Date: Tue, 12 Apr 2016 18:40:38 +0000 Subject: [Histonet] PCP controll swap Message-ID: Hello everyone, I was wondering if anyone want to swap H. Pylori blocks for PCP blocks, (Pneumocystis Carinii)? I could trade other blocks if need too. Thank you :) Victoria R Verno, HT ASCP Hampton Roads Histology Supervisor Desk - 757-398-4763 Fax - 757-397-7475 ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From jvickroy at SpringfieldClinic.com Wed Apr 13 08:14:00 2016 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Wed, 13 Apr 2016 13:14:00 +0000 Subject: [Histonet] Grossing tech qualifications Message-ID: <9B1A1501A800064397369BD8072E6BCA0653E5DB@E2K10DB.springfieldclinic.com> a. We are experiencing trouble finding another tech that will meet CLIA high complexity testing standards as well as has histotechnician training. Also the qualifications for who can gross is a little vague so I wanted to see if anyone has any information that would help me. A summary of the grossing qualifications are listed below. In the past we have hired applicants with bachelor's degrees in biology and then trained them in histotechnology. We also went through the 90 day inhouse training for grossing. My questions have to do with a clarification regarding those with an associate's degree. I have an applicant that has an associate's degree in science with an emphasis on microbiology. My question has to do with the language "An earned associate degree in a laboratory science or medical laboratory technology" . How can I determine what that exactly means? Would a person with an associate's degree in science with an emphasis in microbiology qualify? I'm not sure she has the sixty hours total. Qualifications of a technician for performing gross descriptions and preparation for tissue processing under the direct or indirect supervision of a pathologist include: b. An earned associate degree in a laboratory science or medical laboratory technology, Obtained from an accredited institution, OR c. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, or 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. I would appreciate any understanding that others might have. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From LNormington at uwhealth.org Wed Apr 13 08:37:01 2016 From: LNormington at uwhealth.org (Normington Lacy) Date: Wed, 13 Apr 2016 13:37:01 +0000 Subject: [Histonet] Grossing tech qualifications In-Reply-To: <9B1A1501A800064397369BD8072E6BCA0653E5DB@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA0653E5DB@E2K10DB.springfieldclinic.com> Message-ID: <7F7174244DDD1A49BFBEE8A845BC2F2B1A99E3@UWHC-MBX05.uwhis.hosp.wisc.edu> James, We base all of our high complexity testing on the semester hours as listed in "c." If an individual has completed an associate degree in a laboratory science, they should meet the educational requirements as stated previously. I would recommend obtaining a transcript from the applicants. Our institution then will keep that in their personnel file. As part of our policy for non-PA grossing, I have a form that I complete stating I reviewed the employee's educational requirements. I have copied and pasted my document below. If you are interested in receiving the entire procedure and attachments, please let me know and I can email them to you directly. Employee Name:__________________________ A review of this employee?s educational records was completed on ___________(date). This employee has been ?approved ?not approved to perform high complexity testing based on CLIA requirements as listed in the Federal Register 493.1489 and 1491. 493.1489 states the following education requirements: Each individual performing high complexity testing must-- (b) Meet one of the following requirements: (1) Be a doctor of medicine, doctor of osteopathy, or doctor of podiatric medicine licensed to practice medicine, osteopathy, or podiatry in the State in which the laboratory is located or have earned a doctoral, master's or bachelor's degree in a chemical, physical, biological or clinical laboratory science, or medical technology from an accredited institution; OR (2)(i) Have earned an associate degree in a laboratory science, or medical laboratory technology from an accredited institution or? (ii) Have education and training equivalent to that specified in paragraph (b)(2)(i) of this section that includes-- (A) At least 60 semester hours, or equivalent, from an accredited institution that, at a minimum, include either- (1) 24 semester hours of medical laboratory technology courses; or (2) 24 semester hours of science courses that include-- (i) Six semester hours of chemistry; (ii) Six semester hours of biology; and (iii) Twelve semester hours of chemistry, biology, or medical laboratory technology in any combination Full text on Federal Register qualifications can be viewed at: http://wwwn.cdc.gov/clia/regs/toc.aspx Review completed by: ______________________ (printed name) ______________________ (signature) -----Original Message----- From: Vickroy, James via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 13, 2016 8:14 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Grossing tech qualifications a. We are experiencing trouble finding another tech that will meet CLIA high complexity testing standards as well as has histotechnician training. Also the qualifications for who can gross is a little vague so I wanted to see if anyone has any information that would help me. A summary of the grossing qualifications are listed below. In the past we have hired applicants with bachelor's degrees in biology and then trained them in histotechnology. We also went through the 90 day inhouse training for grossing. My questions have to do with a clarification regarding those with an associate's degree. I have an applicant that has an associate's degree in science with an emphasis on microbiology. My question has to do with the language "An earned associate degree in a laboratory science or medical laboratory technology" . How can I determine what that exactly means? Would a person with an associate's degree in science with an emphasis in microbiology qualify? I'm not sure she has the sixty hours total. Qualifications of a technician for performing gross descriptions and preparation for tissue processing under the direct or indirect supervision of a pathologist include: b. An earned associate degree in a laboratory science or medical laboratory technology, Obtained from an accredited institution, OR c. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, or 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. I would appreciate any understanding that others might have. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From supervisor at galahistolab.com Wed Apr 13 09:15:23 2016 From: supervisor at galahistolab.com (Rachel Pinch) Date: Wed, 13 Apr 2016 09:15:23 -0500 Subject: [Histonet] Grossing tech qualifications In-Reply-To: <7F7174244DDD1A49BFBEE8A845BC2F2B1A99E3@UWHC-MBX05.uwhis.hosp.wisc.edu> References: <9B1A1501A800064397369BD8072E6BCA0653E5DB@E2K10DB.springfieldclinic.com> <7F7174244DDD1A49BFBEE8A845BC2F2B1A99E3@UWHC-MBX05.uwhis.hosp.wisc.edu> Message-ID: <000001d1958e$ebccfeb0$c366fc10$@galahistolab.com> I've had trouble understanding that as well. Can an employee have 24 semester hours of biology and chemistry combined with an associate's degree in science, and it be sufficient for CLIA? Does the person have to have an associates in laboratory science? Rachel -----Original Message----- From: Normington Lacy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 13, 2016 8:37 AM To: 'Vickroy, James' ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Grossing tech qualifications James, We base all of our high complexity testing on the semester hours as listed in "c." If an individual has completed an associate degree in a laboratory science, they should meet the educational requirements as stated previously. I would recommend obtaining a transcript from the applicants. Our institution then will keep that in their personnel file. As part of our policy for non-PA grossing, I have a form that I complete stating I reviewed the employee's educational requirements. I have copied and pasted my document below. If you are interested in receiving the entire procedure and attachments, please let me know and I can email them to you directly. Employee Name:__________________________ A review of this employee?s educational records was completed on ___________(date). This employee has been ?approved ?not approved to perform high complexity testing based on CLIA requirements as listed in the Federal Register 493.1489 and 1491. 493.1489 states the following education requirements: Each individual performing high complexity testing must-- (b) Meet one of the following requirements: (1) Be a doctor of medicine, doctor of osteopathy, or doctor of podiatric medicine licensed to practice medicine, osteopathy, or podiatry in the State in which the laboratory is located or have earned a doctoral, master's or bachelor's degree in a chemical, physical, biological or clinical laboratory science, or medical technology from an accredited institution; OR (2)(i) Have earned an associate degree in a laboratory science, or medical laboratory technology from an accredited institution or? (ii) Have education and training equivalent to that specified in paragraph (b)(2)(i) of this section that includes-- (A) At least 60 semester hours, or equivalent, from an accredited institution that, at a minimum, include either- (1) 24 semester hours of medical laboratory technology courses; or (2) 24 semester hours of science courses that include-- (i) Six semester hours of chemistry; (ii) Six semester hours of biology; and (iii) Twelve semester hours of chemistry, biology, or medical laboratory technology in any combination Full text on Federal Register qualifications can be viewed at: http://wwwn.cdc.gov/clia/regs/toc.aspx Review completed by: ______________________ (printed name) ______________________ (signature) -----Original Message----- From: Vickroy, James via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 13, 2016 8:14 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Grossing tech qualifications a. We are experiencing trouble finding another tech that will meet CLIA high complexity testing standards as well as has histotechnician training. Also the qualifications for who can gross is a little vague so I wanted to see if anyone has any information that would help me. A summary of the grossing qualifications are listed below. In the past we have hired applicants with bachelor's degrees in biology and then trained them in histotechnology. We also went through the 90 day inhouse training for grossing. My questions have to do with a clarification regarding those with an associate's degree. I have an applicant that has an associate's degree in science with an emphasis on microbiology. My question has to do with the language "An earned associate degree in a laboratory science or medical laboratory technology" . How can I determine what that exactly means? Would a person with an associate's degree in science with an emphasis in microbiology qualify? I'm not sure she has the sixty hours total. Qualifications of a technician for performing gross descriptions and preparation for tissue processing under the direct or indirect supervision of a pathologist include: b. An earned associate degree in a laboratory science or medical laboratory technology, Obtained from an accredited institution, OR c. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, or 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. I would appreciate any understanding that others might have. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Melissa.Likens at deaconess.com Wed Apr 13 09:21:34 2016 From: Melissa.Likens at deaconess.com (Melissa Likens) Date: Wed, 13 Apr 2016 14:21:34 +0000 Subject: [Histonet] GLOMERULI ADEQUACY Message-ID: <5C1091A19F87074794AEBDF80EEBD3AA3B2A0584@Exch-MB2.deaconess.com> I have a question about how other institutions handle microscopic evaluation of glomeruli adequacy in renal specimens? Specifically, who at you looks at the cores to determine if glomeruli are present before submitting specimens for further testing? Do the pathologists look at them? Radiologists performing the cores? Other staff? Also, any links or recommendations for training for evaluating renal biopsies for glomeruli would be appreciated. Thanks, Melissa From LNormington at uwhealth.org Wed Apr 13 09:26:26 2016 From: LNormington at uwhealth.org (Normington Lacy) Date: Wed, 13 Apr 2016 14:26:26 +0000 Subject: [Histonet] Grossing tech qualifications In-Reply-To: <000001d1958e$ebccfeb0$c366fc10$@galahistolab.com> References: <9B1A1501A800064397369BD8072E6BCA0653E5DB@E2K10DB.springfieldclinic.com> <7F7174244DDD1A49BFBEE8A845BC2F2B1A99E3@UWHC-MBX05.uwhis.hosp.wisc.edu> <000001d1958e$ebccfeb0$c366fc10$@galahistolab.com> Message-ID: <7F7174244DDD1A49BFBEE8A845BC2F2B1A9A64@UWHC-MBX05.uwhis.hosp.wisc.edu> Essentially, an associates degree totals 60-80 semester hours depending on the program. As long as 24 of those hours are in science or medical laboratory technology, they would qualify. I think the confusion in "c." comes in when a student starts towards a bachelors degree, but then stops the program. As long as they have 60 semester hours with 24 being in science, they too would qualify even though they have no degree. Hope this helps. Lacy -----Original Message----- From: Rachel Pinch [mailto:supervisor at galahistolab.com] Sent: Wednesday, April 13, 2016 9:15 AM To: Normington Lacy; 'Vickroy, James'; histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] Grossing tech qualifications I've had trouble understanding that as well. Can an employee have 24 semester hours of biology and chemistry combined with an associate's degree in science, and it be sufficient for CLIA? Does the person have to have an associates in laboratory science? Rachel -----Original Message----- From: Normington Lacy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 13, 2016 8:37 AM To: 'Vickroy, James' ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Grossing tech qualifications James, We base all of our high complexity testing on the semester hours as listed in "c." If an individual has completed an associate degree in a laboratory science, they should meet the educational requirements as stated previously. I would recommend obtaining a transcript from the applicants. Our institution then will keep that in their personnel file. As part of our policy for non-PA grossing, I have a form that I complete stating I reviewed the employee's educational requirements. I have copied and pasted my document below. If you are interested in receiving the entire procedure and attachments, please let me know and I can email them to you directly. Employee Name:__________________________ A review of this employee?s educational records was completed on ___________(date). This employee has been ?approved ?not approved to perform high complexity testing based on CLIA requirements as listed in the Federal Register 493.1489 and 1491. 493.1489 states the following education requirements: Each individual performing high complexity testing must-- (b) Meet one of the following requirements: (1) Be a doctor of medicine, doctor of osteopathy, or doctor of podiatric medicine licensed to practice medicine, osteopathy, or podiatry in the State in which the laboratory is located or have earned a doctoral, master's or bachelor's degree in a chemical, physical, biological or clinical laboratory science, or medical technology from an accredited institution; OR (2)(i) Have earned an associate degree in a laboratory science, or medical laboratory technology from an accredited institution or? (ii) Have education and training equivalent to that specified in paragraph (b)(2)(i) of this section that includes-- (A) At least 60 semester hours, or equivalent, from an accredited institution that, at a minimum, include either- (1) 24 semester hours of medical laboratory technology courses; or (2) 24 semester hours of science courses that include-- (i) Six semester hours of chemistry; (ii) Six semester hours of biology; and (iii) Twelve semester hours of chemistry, biology, or medical laboratory technology in any combination Full text on Federal Register qualifications can be viewed at: http://wwwn.cdc.gov/clia/regs/toc.aspx Review completed by: ______________________ (printed name) ______________________ (signature) -----Original Message----- From: Vickroy, James via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 13, 2016 8:14 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Grossing tech qualifications a. We are experiencing trouble finding another tech that will meet CLIA high complexity testing standards as well as has histotechnician training. Also the qualifications for who can gross is a little vague so I wanted to see if anyone has any information that would help me. A summary of the grossing qualifications are listed below. In the past we have hired applicants with bachelor's degrees in biology and then trained them in histotechnology. We also went through the 90 day inhouse training for grossing. My questions have to do with a clarification regarding those with an associate's degree. I have an applicant that has an associate's degree in science with an emphasis on microbiology. My question has to do with the language "An earned associate degree in a laboratory science or medical laboratory technology" . How can I determine what that exactly means? Would a person with an associate's degree in science with an emphasis in microbiology qualify? I'm not sure she has the sixty hours total. Qualifications of a technician for performing gross descriptions and preparation for tissue processing under the direct or indirect supervision of a pathologist include: b. An earned associate degree in a laboratory science or medical laboratory technology, Obtained from an accredited institution, OR c. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, or 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. I would appreciate any understanding that others might have. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Wed Apr 13 10:08:14 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 13 Apr 2016 15:08:14 +0000 Subject: [Histonet] GLOMERULI ADEQUACY In-Reply-To: <5C1091A19F87074794AEBDF80EEBD3AA3B2A0584@Exch-MB2.deaconess.com> References: <5C1091A19F87074794AEBDF80EEBD3AA3B2A0584@Exch-MB2.deaconess.com> Message-ID: <761E2B5697F795489C8710BCC72141FF6FD3B1A7@ex07.net.ucsf.edu> Melissa, our PA's look at them when received in the grossing lab, and if they aren't sure they bring them down the hall to the EM lab and we confirm. Our clinicians seem to be pretty good at getting a good bx without examining them in radiology so this works for us. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Melissa Likens via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 13, 2016 7:22 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] GLOMERULI ADEQUACY I have a question about how other institutions handle microscopic evaluation of glomeruli adequacy in renal specimens? Specifically, who at you looks at the cores to determine if glomeruli are present before submitting specimens for further testing? Do the pathologists look at them? Radiologists performing the cores? Other staff? Also, any links or recommendations for training for evaluating renal biopsies for glomeruli would be appreciated. Thanks, Melissa _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LNormington at uwhealth.org Wed Apr 13 10:51:52 2016 From: LNormington at uwhealth.org (Normington Lacy) Date: Wed, 13 Apr 2016 15:51:52 +0000 Subject: [Histonet] GLOMERULI ADEQUACY In-Reply-To: <5C1091A19F87074794AEBDF80EEBD3AA3B2A0584@Exch-MB2.deaconess.com> References: <5C1091A19F87074794AEBDF80EEBD3AA3B2A0584@Exch-MB2.deaconess.com> Message-ID: <7F7174244DDD1A49BFBEE8A845BC2F2B1A9AF4@UWHC-MBX05.uwhis.hosp.wisc.edu> We have our histology technicians attend all native and transplant renal biopsy procedures. Our institution has stereo scopes (dissecting scope) located in ultrasound, transplant clinic and an additional one on a mobile cart for cases that might occur in Pediatric sedation, ICU, or bedside biopsies. The core biopsy is handed to the histology technician who then looks under the scope to look for glomeruli. Some of our clinicians like an approximate glomeruli count, although, most just want to know if they have cortical tissue. In each of our reports we place the following comment per CAP regulations regarding adequacy (see below). If a sample is difficult to determine adequacy, we always leave it up to the Nephrologist discretion. We have a lead technician in the renal laboratory. This individual trains all new employees on adequacy assessment. Modern Pathology has an excellent photo of the difference between cortex and medullary tissue. Search google images for dissecting scope renal biopsy. ____ At the time of sample collection, this sample visibly contains cortical tissue and is Satisfactory for Evaluation. ____ At the time of sample collection, cortical tissue is not identifiable and is Unsatisfactory for Evaluation. Lacy -----Original Message----- From: Melissa Likens via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 13, 2016 9:22 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] GLOMERULI ADEQUACY I have a question about how other institutions handle microscopic evaluation of glomeruli adequacy in renal specimens? Specifically, who at you looks at the cores to determine if glomeruli are present before submitting specimens for further testing? Do the pathologists look at them? Radiologists performing the cores? Other staff? Also, any links or recommendations for training for evaluating renal biopsies for glomeruli would be appreciated. Thanks, Melissa _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun at hhchealth.org Wed Apr 13 11:06:33 2016 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Wed, 13 Apr 2016 16:06:33 +0000 Subject: [Histonet] GLOMERULI ADEQUACY In-Reply-To: <5C1091A19F87074794AEBDF80EEBD3AA3B2A0584@Exch-MB2.deaconess.com> References: <5C1091A19F87074794AEBDF80EEBD3AA3B2A0584@Exch-MB2.deaconess.com> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B0C1A5B@HHCEXCHMB03.hhcsystem.org> We provide on-site assessment of the renal tissue obtained in Ultrasound, both at our main hospital and at our Children's hospital across the street. I handle probably 95% of the biopsies; I have two pathology colleagues (MDs) that handle the other 5% when I am not here. Occasionally, a resident or fellow (trained by me) will handle a biopsy after-hours. We place the core(s) on a glass slide with a few drops of saline or RPMI and then examine it under a microscope using 4x and 10x objectives in Ultrasound. We tell the interventional radiologist/PA or the nephrologist performing the procedure when the specimen is adequate. We usually obtain adequate tissue with 2, 18-gauge needle cores in more than 90% of the procedures; sometimes we need to ask for a 3rd core. On the other hand, I can make one core work when it's more than 2.0 cm long if there's cortex at both ends. However, I don't recommend obtaining cores this long. We have not had to repeat a biopsy over the last 25 years. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Melissa Likens via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 13, 2016 10:22 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] GLOMERULI ADEQUACY I have a question about how other institutions handle microscopic evaluation of glomeruli adequacy in renal specimens? Specifically, who at you looks at the cores to determine if glomeruli are present before submitting specimens for further testing? Do the pathologists look at them? Radiologists performing the cores? Other staff? Also, any links or recommendations for training for evaluating renal biopsies for glomeruli would be appreciated. Thanks, Melissa _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From JRobinson at pathology-associates.com Wed Apr 13 11:43:19 2016 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Wed, 13 Apr 2016 16:43:19 +0000 Subject: [Histonet] GLOMERULI ADEQUACY In-Reply-To: <5C1091A19F87074794AEBDF80EEBD3AA3B2A0584@Exch-MB2.deaconess.com> References: <5C1091A19F87074794AEBDF80EEBD3AA3B2A0584@Exch-MB2.deaconess.com> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C90A1522C@PAEXCH1.PathologyAssociates.local> Hi Melissa- when Tim Morken taught me to be an EM tech many moons ago we would take a small dissecting microscope to the CT room. We would have the radiologist place the first core on a sterile tongue depressor and then we would check it in some saline on a dental wax square under the scope. The viable gloms were quite easy to see. We could ask for an additional core or two right then if we felt it was needed. We would then place the cores in a saline vial and take them back to our lab to split up the cores into the appropriate fixatives. It really was quite valuable to have that interaction with the radiologists and to have the ability to ensure that we were obtaining adequate samples with the dual goals of giving ourselves enough tissue to perform the EM workup as well as avoiding any repeat biopsies due to inadequate sampling. Jeff Robinson, Senior Histotechnologist, EM Tech Emeritus, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Melissa Likens via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 13, 2016 7:22 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] GLOMERULI ADEQUACY I have a question about how other institutions handle microscopic evaluation of glomeruli adequacy in renal specimens? Specifically, who at you looks at the cores to determine if glomeruli are present before submitting specimens for further testing? Do the pathologists look at them? Radiologists performing the cores? Other staff? Also, any links or recommendations for training for evaluating renal biopsies for glomeruli would be appreciated. Thanks, Melissa _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From fatimaformadi at gmail.com Wed Apr 13 11:47:23 2016 From: fatimaformadi at gmail.com (Fatima Formadi) Date: Wed, 13 Apr 2016 10:47:23 -0600 Subject: [Histonet] Frozen tissues Message-ID: Hi Everyone, I am new to the net and this is my first post. I am a fairly new lab tech who will soon start working in the histology department of our hospital and I was wondering if you veterans can give me some heads up. I will be working mainly with frozen tissues. 1. Are the main stains for frozen tissues the following: rapid H&E, PAS, and methylene blue? 2. Does anyone have a protocol for collecting and disposing of reagents (stains, acids, alcohols etc.) that they might share with me? 3. After preparing the slides for the pathologist, is the remaining tissue always frozen in liquid nitrogen? Thanks for any input. Fatima From LRaff at uropartners.com Wed Apr 13 13:38:09 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 13 Apr 2016 18:38:09 +0000 Subject: [Histonet] Blog Post Not lab related Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10D02DB3@COLOEXCH01.uropartners.local> Just some philosophy towards the end of a long day. http://www.chicagonow.com/downsize-maybe/2016/04/kitten-power-can-get-things-done/ Just a reminder-I try to limit my blog invitations here. If you enjoy the blog, remember to subscribe (no charge, no spam) on the ChicagoNow blog site. Thanks for your readership. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From mills at 3scan.com Wed Apr 13 14:11:21 2016 From: mills at 3scan.com (Caroline Miller) Date: Wed, 13 Apr 2016 12:11:21 -0700 Subject: [Histonet] Blog Post Not lab related In-Reply-To: <6347C6D2B080534F9B5C2B08436DCFAF10D02DB3@COLOEXCH01.uropartners.local> References: <6347C6D2B080534F9B5C2B08436DCFAF10D02DB3@COLOEXCH01.uropartners.local> Message-ID: Lester, I do believe this is the second 'non lab related' blog post this week. As we have spoken about before I do not think histonet is an appropriate place for your blog posts. I, personally, was totally OK with you co-posting with other lab related topics. However you are now pushing it and I respectfully ask that you refrain from sending out your blog posts to the whole list, let people sign up if they are interested. The issue is that this is a histology related list, if everyone posted non-lab stuff here it would soon be chaos! Respectfully yours, mills On Wed, Apr 13, 2016 at 11:38 AM, Lester Raff MD via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Just some philosophy towards the end of a long day. > > > http://www.chicagonow.com/downsize-maybe/2016/04/kitten-power-can-get-things-done/ > > > > Just a reminder-I try to limit my blog invitations here. If you enjoy the > blog, remember to subscribe (no charge, no spam) on the ChicagoNow blog > site. > > Thanks for your readership. > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From rjbuesa at yahoo.com Thu Apr 14 08:01:28 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 14 Apr 2016 13:01:28 +0000 (UTC) Subject: [Histonet] Blog Post Not lab related In-Reply-To: References: Message-ID: <1949585274.349912.1460638888342.JavaMail.yahoo@mail.yahoo.com> Amen!!!But you have to concede that Lester is a very? persistent, almost obstinate individual, probably used to impose his will and this postings are?just an example of it: he likes his blog and tries to impose it to everyone. Evidently he has all the time in the world and just does not know what to do with it and enjoys sharing his "witty" side.?Ren? On Wednesday, April 13, 2016 3:24 PM, Caroline Miller via Histonet wrote: Lester, I do believe this is the second 'non lab related' blog post this week. As we have spoken about before I do not think histonet is an appropriate place for your blog posts. I, personally, was totally OK with you co-posting with other lab related topics. However you are now pushing it and I respectfully ask that you refrain from sending out your blog posts to the whole list, let people sign up if they are interested. The issue is that this is a histology related list, if everyone posted non-lab stuff here it would soon be chaos! Respectfully yours, mills On Wed, Apr 13, 2016 at 11:38 AM, Lester Raff MD via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Just some philosophy towards the end of a long day. > > > http://www.chicagonow.com/downsize-maybe/2016/04/kitten-power-can-get-things-done/ > > > > Just a reminder-I try to limit my blog invitations here. If you enjoy the > blog, remember to subscribe (no charge, no spam) on the ChicagoNow blog > site. > > Thanks for your readership. > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff at rwjms.rutgers.edu Thu Apr 14 09:18:45 2016 From: mcauliff at rwjms.rutgers.edu (Geoff) Date: Thu, 14 Apr 2016 10:18:45 -0400 Subject: [Histonet] Blog Post Not lab related In-Reply-To: <1949585274.349912.1460638888342.JavaMail.yahoo@mail.yahoo.com> References: <1949585274.349912.1460638888342.JavaMail.yahoo@mail.yahoo.com> Message-ID: <570FA6C5.3020404@umdnj.edu> I automatically delete all of his posts. Geoff On 4/14/2016 9:01 AM, Rene J Buesa via Histonet wrote: > Amen!!!But you have to concede that Lester is a very persistent, almost obstinate individual, probably used to impose his will and this postings are just an example of it: he likes his blog and tries to impose it to everyone. Evidently he has all the time in the world and just does not know what to do with it and enjoys sharing his "witty" side. Ren? > > On Wednesday, April 13, 2016 3:24 PM, Caroline Miller via Histonet wrote: > > > Lester, I do believe this is the second 'non lab related' blog post this > week. As we have spoken about before I do not think histonet is an > appropriate place for your blog posts. I, personally, was totally OK with > you co-posting with other lab related topics. However you are now pushing > it and I respectfully ask that you refrain from sending out your blog posts > to the whole list, let people sign up if they are interested. > > The issue is that this is a histology related list, if everyone posted > non-lab stuff here it would soon be chaos! > > Respectfully yours, > mills > > On Wed, Apr 13, 2016 at 11:38 AM, Lester Raff MD via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Just some philosophy towards the end of a long day. >> >> >> http://www.chicagonow.com/downsize-maybe/2016/04/kitten-power-can-get-things-done/ >> >> >> >> Just a reminder-I try to limit my blog invitations here. If you enjoy the >> blog, remember to subscribe (no charge, no spam) on the ChicagoNow blog >> site. >> >> Thanks for your readership. >> >> Lester J. Raff, MD MBA >> UroPartners >> Medical Director Of Laboratory >> 2225 Enterprise Dr. Suite 2511 >> Westchester, Il 60154 >> Tel: 708-486-0076 >> Fax: 708-492-0203 >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff at rwjms.rutgers.edu ********************************************** From JMacDonald at mtsac.edu Thu Apr 14 11:16:08 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Thu, 14 Apr 2016 09:16:08 -0700 Subject: [Histonet] Blog Post Not lab related In-Reply-To: <1949585274.349912.1460638888342.JavaMail.yahoo@mail.yahoo.com> References: <1949585274.349912.1460638888342.JavaMail.yahoo@mail.yahoo.com> Message-ID: In addition to the Histonet, I am on a listserve for clinical laboratory educators. Dr. Raff posts his blog link on that listserve as well. He also responds to some of the questions. There has never been one public complaint regarding his posts. It is very clear in the subject line and the ability to delete his posts are quite easy. Please don't give credence to the myth that the clinical lab has more patience and common sense than the histology world. From: Rene J Buesa via Histonet To: Caroline Miller , Lester Raff MD Cc: "histonet at lists.utsouthwestern.edu" Date: 04/14/2016 06:02 AM Subject: Re: [Histonet] Blog Post Not lab related Amen!!!But you have to concede that Lester is a very persistent, almost obstinate individual, probably used to impose his will and this postings are just an example of it: he likes his blog and tries to impose it to everyone. Evidently he has all the time in the world and just does not know what to do with it and enjoys sharing his "witty" side. Ren? On Wednesday, April 13, 2016 3:24 PM, Caroline Miller via Histonet wrote: Lester, I do believe this is the second 'non lab related' blog post this week. As we have spoken about before I do not think histonet is an appropriate place for your blog posts. I, personally, was totally OK with you co-posting with other lab related topics. However you are now pushing it and I respectfully ask that you refrain from sending out your blog posts to the whole list, let people sign up if they are interested. The issue is that this is a histology related list, if everyone posted non-lab stuff here it would soon be chaos! Respectfully yours, mills On Wed, Apr 13, 2016 at 11:38 AM, Lester Raff MD via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Just some philosophy towards the end of a long day. > > > http://www.chicagonow.com/downsize-maybe/2016/04/kitten-power-can-get-things-done/ > > > > Just a reminder-I try to limit my blog invitations here. If you enjoy the > blog, remember to subscribe (no charge, no spam) on the ChicagoNow blog > site. > > Thanks for your readership. > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward at wakehealth.edu Thu Apr 14 11:26:23 2016 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Thu, 14 Apr 2016 16:26:23 +0000 Subject: [Histonet] Blog Post Not lab related In-Reply-To: References: <1949585274.349912.1460638888342.JavaMail.yahoo@mail.yahoo.com> Message-ID: I agree....the delete button is very easy to use. -----Original Message----- From: Jennifer MacDonald via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, April 14, 2016 12:16 PM To: Rene J Buesa Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Blog Post Not lab related In addition to the Histonet, I am on a listserve for clinical laboratory educators. Dr. Raff posts his blog link on that listserve as well. He also responds to some of the questions. There has never been one public complaint regarding his posts. It is very clear in the subject line and the ability to delete his posts are quite easy. Please don't give credence to the myth that the clinical lab has more patience and common sense than the histology world. From: Rene J Buesa via Histonet To: Caroline Miller , Lester Raff MD Cc: "histonet at lists.utsouthwestern.edu" Date: 04/14/2016 06:02 AM Subject: Re: [Histonet] Blog Post Not lab related Amen!!!But you have to concede that Lester is a very persistent, almost obstinate individual, probably used to impose his will and this postings are just an example of it: he likes his blog and tries to impose it to everyone. Evidently he has all the time in the world and just does not know what to do with it and enjoys sharing his "witty" side. Ren? On Wednesday, April 13, 2016 3:24 PM, Caroline Miller via Histonet wrote: Lester, I do believe this is the second 'non lab related' blog post this week. As we have spoken about before I do not think histonet is an appropriate place for your blog posts. I, personally, was totally OK with you co-posting with other lab related topics. However you are now pushing it and I respectfully ask that you refrain from sending out your blog posts to the whole list, let people sign up if they are interested. The issue is that this is a histology related list, if everyone posted non-lab stuff here it would soon be chaos! Respectfully yours, mills On Wed, Apr 13, 2016 at 11:38 AM, Lester Raff MD via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Just some philosophy towards the end of a long day. > > > http://www.chicagonow.com/downsize-maybe/2016/04/kitten-power-can-get-things-done/ > > > > Just a reminder-I try to limit my blog invitations here. If you enjoy the > blog, remember to subscribe (no charge, no spam) on the ChicagoNow blog > site. > > Thanks for your readership. > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From steve8438 at gmail.com Thu Apr 14 11:34:44 2016 From: steve8438 at gmail.com (Steve Mello) Date: Thu, 14 Apr 2016 12:34:44 -0400 Subject: [Histonet] Blog Post Not lab related In-Reply-To: References: <1949585274.349912.1460638888342.JavaMail.yahoo@mail.yahoo.com> Message-ID: <20160414163444.4403283.12109.21610@gmail.com> If you do not like his post which he clearly denotes in the subject line hit "delete". Being rude to him is offensive to me as a professional which clearly some of you are not acting/writing as such. Would we call our pathologist employers "Amen!!!But you have to concede that Lester is a very persistent, almost obstinate individual, probably used to impose his will and this postings? are just an example of it: he likes his blog and tries to impose it to? everyone. Evidently he has all the time in the world and just does not? know what to do with it and enjoys sharing his "witty" side. Ren??" Even in some "histology" post I find many responses to our colleagues rude and unacceptable. ? I personally think this subject should have been sent directly to Dr. Raff not the entire listserver. But I guess people just need to Grandstand My thoughts? Steven Mello, HT(ASCP) Sent?from?my?BlackBerry?10?smartphone. ? Original Message ? From: Jennifer MacDonald via Histonet Sent: Thursday, April 14, 2016 12:22 PM To: Rene J Buesa Reply To: Jennifer MacDonald Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Blog Post Not lab related In addition to the Histonet, I am on a listserve for clinical laboratory educators. Dr. Raff posts his blog link on that listserve as well. He also responds to some of the questions. There has never been one public complaint regarding his posts. It is very clear in the subject line and the ability to delete his posts are quite easy. Please don't give credence to the myth that the clinical lab has more patience and common sense than the histology world. From: Rene J Buesa via Histonet To: Caroline Miller , Lester Raff MD Cc: "histonet at lists.utsouthwestern.edu" Date: 04/14/2016 06:02 AM Subject: Re: [Histonet] Blog Post Not lab related Amen!!!But you have to concede that Lester is a very persistent, almost ? obstinate individual, probably used to impose his will and this postings are just an example of it: he likes his blog and tries to impose it to everyone. Evidently he has all the time in the world and just does not know what to do with it and enjoys sharing his "witty" side. Ren? On Wednesday, April 13, 2016 3:24 PM, Caroline Miller via Histonet wrote: Lester, I do believe this is the second 'non lab related' blog post this week. As we have spoken about before I do not think histonet is an appropriate place for your blog posts. I, personally, was totally OK with you co-posting with other lab related topics. However you are now pushing it and I respectfully ask that you refrain from sending out your blog posts to the whole list, let people sign up if they are interested. The issue is that this is a histology related list, if everyone posted non-lab stuff here it would soon be chaos! Respectfully yours, mills On Wed, Apr 13, 2016 at 11:38 AM, Lester Raff MD via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Just some philosophy towards the end of a long day. > > > http://www.chicagonow.com/downsize-maybe/2016/04/kitten-power-can-get-things-done/ > > > > Just a reminder-I try to limit my blog invitations here. If you enjoy the > blog, remember to subscribe (no charge, no spam) on the ChicagoNow blog > site. > > Thanks for your readership. > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cdemarinis at SARATOGACARE.ORG Thu Apr 14 12:42:00 2016 From: cdemarinis at SARATOGACARE.ORG (Demarinis, Carolyn) Date: Thu, 14 Apr 2016 17:42:00 +0000 Subject: [Histonet] Pathologist special requests Message-ID: <1C75A843982A7B44BB368A3CC946ABCA012D5FD829@SHEXCHMBX01.SARAHOSP.ORG> Meditech Magic 5.66 users - Anatomic Pathology module: Is there an easy process for the pathologists to electronically send special request orders (special stains, recuts, send outs, etc.) to the histology department printer when they are reviewing the slides and patient's record in Anatomic Pathology module in Meditech? Our current process is the pathologist has a template and they type the surgical # and special request, print a copy to their printer, and walk to histology to deliver the request. -- This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy at saratogacare.org and destroy all copies of this communication and any attachments. From heather.brown.9 at us.af.mil Thu Apr 14 13:01:05 2016 From: heather.brown.9 at us.af.mil (BROWN, HEATHER L GS-07 USAF AETC 59 CRD/SGVUO) Date: Thu, 14 Apr 2016 18:01:05 +0000 Subject: [Histonet] Blog Post Not lab related Message-ID: <18001CBA5CE3B344A08EF7D50F6CC6555F2A85EC@52VEJX-D07-05C.area52.afnoapps.usaf.mil> I am new to the Histonet, not to histology though. There have been 2 great question and answer sessions since I've been on here. The way some of you guys talk to each other is so disrespectful. I went through AFIP as a civilian years ago and I thought the histo world was a small society of people who shared a common, if not morbid, curiosity of the human anatomy, how it works and how can we do the best we can do to help our patient. I will read all the submissions, because there may actually be a good answer to a question. I will have to really weigh my options with asking a question...cruelty and ridicule or the possibility of a good answer? If you don't like histo, then don't do histo. If you can't be nice, then say nothing at all. Life is a beat down everyday, I darn sure wouldn't need if from my peers too. Heather L. Brown, HT, ASCP JBSA-Lackland, Texas From Megan.Dishop at childrensmn.org Thu Apr 14 13:37:38 2016 From: Megan.Dishop at childrensmn.org (Megan Dishop) Date: Thu, 14 Apr 2016 13:37:38 -0500 Subject: [Histonet] Blog Post Not lab related In-Reply-To: <18001CBA5CE3B344A08EF7D50F6CC6555F2A85EC@52VEJX-D07-05C.area52.afnoapps.usaf.mil> References: <18001CBA5CE3B344A08EF7D50F6CC6555F2A85EC@52VEJX-D07-05C.area52.afnoapps.usaf.mil> Message-ID: <570F9DE3020000E30002204C@vcgwia1.childrenshc.org> Thank you for bringing attention to this, Heather. This has been my observation also, and I couldn't agree more. Let's keep it professional. Sent from my Sprint Samsung Galaxy? Note 4. -------- Original message -------- From: "BROWN, HEATHER L GS-07 USAF AETC 59 CRD/SGVUO via Histonet" Date: 4/14/16 1:02 PM (GMT-06:00) To: histonet at lists.utsouthwestern.edu, HEATHER L GS-07 USAF AETC 59 CRD/SGVUO BROWN Subject: Re: [Histonet] Blog Post Not lab related >>> "BROWN, HEATHER L GS-07 USAF AETC 59 CRD/SGVUO via Histonet" 2016-04-14T13:02:26.146402 >>> I am new to the Histonet, not to histology though. There have been 2 great question and answer sessions since I've been on here. The way some of you guys talk to each other is so disrespectful. I went through AFIP as a civilian years ago and I thought the histo world was a small society of people who shared a common, if not morbid, curiosity of the human anatomy, how it works and how can we do the best we can do to help our patient. I will read all the submissions, because there may actually be a good answer to a question. I will have to really weigh my options with asking a question...cruelty and ridicule or the possibility of a good answer? If you don't like histo, then don't do histo. If you can't be nice, then say nothing at all. Life is a beat down everyday, I darn sure wouldn't need if from my peers too. Heather L. Brown, HT, ASCP JBSA-Lackland, Texas _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. From wdesalvo.cac at outlook.com Thu Apr 14 15:20:42 2016 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Thu, 14 Apr 2016 15:20:42 -0500 Subject: [Histonet] Blog Post Not lab related Message-ID: IMO, this is a list serve not an advertising space. Keep blog posts and other advertising off the list. Keep the exchange about histotechnology. Plenty of interweb space for other communication. Dr. Raff and everyone is always welcome to be part of the discussion and help get answers to those that need. Sent from my Windows Phone ________________________________ From: Megan Dishop via Histonet Sent: ?4/?14/?2016 2:01 PM To: histonet at lists.utsouthwestern.edu; heather.brown.9 at us.af.mil Subject: Re: [Histonet] Blog Post Not lab related Thank you for bringing attention to this, Heather. This has been my observation also, and I couldn't agree more. Let's keep it professional. Sent from my Sprint Samsung Galaxy? Note 4. -------- Original message -------- From: "BROWN, HEATHER L GS-07 USAF AETC 59 CRD/SGVUO via Histonet" Date: 4/14/16 1:02 PM (GMT-06:00) To: histonet at lists.utsouthwestern.edu, HEATHER L GS-07 USAF AETC 59 CRD/SGVUO BROWN Subject: Re: [Histonet] Blog Post Not lab related >>> "BROWN, HEATHER L GS-07 USAF AETC 59 CRD/SGVUO via Histonet" 2016-04-14T13:02:26.146402 >>> I am new to the Histonet, not to histology though. There have been 2 great question and answer sessions since I've been on here. The way some of you guys talk to each other is so disrespectful. I went through AFIP as a civilian years ago and I thought the histo world was a small society of people who shared a common, if not morbid, curiosity of the human anatomy, how it works and how can we do the best we can do to help our patient. I will read all the submissions, because there may actually be a good answer to a question. I will have to really weigh my options with asking a question...cruelty and ridicule or the possibility of a good answer? If you don't like histo, then don't do histo. If you can't be nice, then say nothing at all. Life is a beat down everyday, I darn sure wouldn't need if from my peers too. Heather L. Brown, HT, ASCP JBSA-Lackland, Texas _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Statement: This email/fax, including attachments, may include confidential and/or proprietary information and may be used only by the person or entity to which it is addressed. If the reader of this email/fax is not the intended recipient or his or her agent, the reader is hereby notified that any dissemination, distribution or copying of this email/fax is prohibited. If you have received this email/fax in error, please notify the sender by replying to this message and deleting this email or destroying this facsimile immediately. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fatimaformadi at gmail.com Fri Apr 15 09:36:05 2016 From: fatimaformadi at gmail.com (Fatima Formadi) Date: Fri, 15 Apr 2016 08:36:05 -0600 Subject: [Histonet] Frozen tissues Message-ID: Hi Everyone, Sorry I was not clear in my last post. The frozen tissues are standard frozens for quick diagnosis. All inputs will be greatly appreciated. Thanks Fatima From hhawkins at UTMB.EDU Fri Apr 15 14:07:40 2016 From: hhawkins at UTMB.EDU (Hawkins, Hal K.) Date: Fri, 15 Apr 2016 19:07:40 +0000 Subject: [Histonet] specimen submission pads In-Reply-To: <7898c70581034cd7b8f31a1827b78f73@MBX10.CCHCS.LDAP> References: <7898c70581034cd7b8f31a1827b78f73@MBX10.CCHCS.LDAP> Message-ID: <22624908330375439D6382C9F95093FF9454CAD9@GRMBX1.utmb.edu> Way back when I was at Emory, the GI docs used to place their biopsies on freshly cut slices of cucumber. The cucumber slices were fixed and processed with the specimen, cut nicely, and support the tissue in a perfectly flat orientation. I discourage use of the standard blue gauze pads because of the distortion they cause in fresh tissue samples, the notorious triangular holes. ________________________________________ From: Linda Margraf via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 06, 2016 11:46 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] specimen submission pads Hi Histonetters, We are having trouble in the Gross room, removing small specimens from the pads/gauze that OR personnel put them on which they then immerse in formalin. We thought the gauze was challenging to remove tiny specimens from but have found that the Telfa pads the OR is now using come apart in the fixative and are even more challenging to work with. The GI lab uses sponges but they would be too small for the usual size containers the OR send us. Any suggestions? Thanks in advance, Linda M _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From eddessa at emory.edu Fri Apr 15 17:08:59 2016 From: eddessa at emory.edu (Dessasau III, Evan) Date: Fri, 15 Apr 2016 22:08:59 +0000 Subject: [Histonet] specimen submission pads In-Reply-To: <22624908330375439D6382C9F95093FF9454CAD9@GRMBX1.utmb.edu> References: <7898c70581034cd7b8f31a1827b78f73@MBX10.CCHCS.LDAP> <22624908330375439D6382C9F95093FF9454CAD9@GRMBX1.utmb.edu> Message-ID: We use a product from EMS called Cellsafe biopsy insert CAT.62327-10. I try to remember to give them to the Fellows on the transplant team when doing kidney needle biopsies. They do not fit in the EZ Load cassettes but I keep other cassettes which hold the insert just fine. E-van -----Original Message----- From: Hawkins, Hal K. via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, April 15, 2016 3:08 PM To: Linda Margraf; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] specimen submission pads Way back when I was at Emory, the GI docs used to place their biopsies on freshly cut slices of cucumber. The cucumber slices were fixed and processed with the specimen, cut nicely, and support the tissue in a perfectly flat orientation. I discourage use of the standard blue gauze pads because of the distortion they cause in fresh tissue samples, the notorious triangular holes. ________________________________________ From: Linda Margraf via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 06, 2016 11:46 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] specimen submission pads Hi Histonetters, We are having trouble in the Gross room, removing small specimens from the pads/gauze that OR personnel put them on which they then immerse in formalin. We thought the gauze was challenging to remove tiny specimens from but have found that the Telfa pads the OR is now using come apart in the fixative and are even more challenging to work with. The GI lab uses sponges but they would be too small for the usual size containers the OR send us. Any suggestions? Thanks in advance, Linda M _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Lisa.White3 at va.gov Mon Apr 18 08:11:41 2016 From: Lisa.White3 at va.gov (White, Lisa M.) Date: Mon, 18 Apr 2016 09:11:41 -0400 Subject: [Histonet] specimen submission pads Message-ID: <2B2ECF33934F5D4996D8BE03EFDF39760C478B62@VHAV09MSGA3.v09.med.va.gov> Have they tried not using any pads? Our biopsies (GI, Needle Core, Prostate etc.) are just in the formalin specimen bottle. We don't have any issues with the "free floating" specimens. I have worked at several labs and never seen specimens routinely submitted on a gauze or pad. Just a thought. Lisa From tbraud at holyredeemer.com Mon Apr 18 08:26:42 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 18 Apr 2016 13:26:42 +0000 Subject: [Histonet] Specimen submission pads Message-ID: <48E053DDF6CE074DB6A7414BA05403F806E84E@HRHEX02-HOS.holyredeemer.local> We, too, had the same problem. The GI rooms were cutting telfa pads so they would fit in the formalin containers, and the pads were falling apart. We finally asked that they just put the specimens directly into the formalin. That way we can decide in the gross room, the best way to submit the tissue. Mesh bags, lens paper, or foam sponges. We've found that well fixed GI biopsies can easily be processed using "foam sponges" and are easy to retrieve for embedding and show no "foam pad cut artifact". We use Mesh bags for multiple tiny particles, and lens paper for tiny individual fragments. I hope this helps. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Today's Topics: ________________________________________ From: Linda Margraf via Histonet [histonet at lists.utsouthwestern.edu] Hi Histonetters, We are having trouble in the Gross room, removing small specimens from the pads/gauze that OR personnel put them on which they then immerse in formalin. We thought the gauze was challenging to remove tiny specimens from but have found that the Telfa pads the OR is now using come apart in the fixative and are even more challenging to work with. The GI lab uses sponges but they would be too small for the usual size containers the OR send us. Any suggestions? Thanks in advance, Linda M *********** From LRaff at uropartners.com Mon Apr 18 08:37:24 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 18 Apr 2016 13:37:24 +0000 Subject: [Histonet] Specimen pads and medical blog post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10D13C0C@COLOEXCH01.uropartners.local> Hal Hawkins wrote: "Way back when I was at Emory, the GI docs used to place their biopsies on freshly cut slices of cucumber. The cucumber slices were fixed and processed with the specimen, cut nicely, and support the tissue in a perfectly flat orientation." We also did that for GI biopsies during my residency and it did do a nice job of maintaining orientation, though I assume it was a bit of a bother for the GI staff. I believe some dye was placed on the cucumber to make it more visible for the GI staff. Not sure if is still done that way at my old hospital. Medical Blog Post-pathology related http://www.chicagonow.com/downsize-maybe/2016/04/when-cancer-is-no-longer-cancer/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From criley at dpspa.com Mon Apr 18 08:42:59 2016 From: criley at dpspa.com (Charles Riley) Date: Mon, 18 Apr 2016 09:42:59 -0400 Subject: [Histonet] Positive control tissue Message-ID: Does anyone have a positive HMBE-1 tissue block they would be willing to send me? Our only block has been exhausted. -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From rsrichmond at gmail.com Mon Apr 18 12:42:23 2016 From: rsrichmond at gmail.com (Bob Richmond) Date: Mon, 18 Apr 2016 13:42:23 -0400 Subject: [Histonet] Specimen submission pads Message-ID: The blue pads shouldn't introduce artifacts if the tissue is properly fixed before placing the tissue on them. One service I worked on marked the specimens on the blue pad with a bit of safranin O solution (the microbiology Gram stain counterstain - don't use eosin, because it's fluorescent). Does anyone use a stereo microscope or other visual aid to orient small bowel biopsy specimens when embedding? I heard of the cucumber technique many years ago, but have never seen it used. As I recall, the cucumber slices weren't used fresh, but were stored in alcohol. Gynecologists used this technique to orient cervical biopsy specimens. Telfa with GI specimens is a dreadful idea - the stuff traps specimens - a major nuisance with 18 gauge needle prostate biopsy specimens. I want NOTHING in the jar with the specimen. Bob Richmond Samurai Pathologist Maryville TN From pdefazio802 at gmail.com Mon Apr 18 18:26:29 2016 From: pdefazio802 at gmail.com (Pam DeFazio) Date: Mon, 18 Apr 2016 19:26:29 -0400 Subject: [Histonet] Dako Artisan stainer Message-ID: Does anyone have experience with using the deparaffinization feature? Currently we are deparaffinizing by hand. Any problems with the stain quality or other issues? From mills at 3scan.com Tue Apr 19 09:41:43 2016 From: mills at 3scan.com (Caroline Miller) Date: Tue, 19 Apr 2016 07:41:43 -0700 Subject: [Histonet] Paper request please Message-ID: I can't find this anywhere: Anal Quant Cytol Histol. 2001 Aug;23(4):291-9. Quantification of histochemical staining by color deconvolution. Ruifrok AC 1, Johnston DA . Does anyone have a copy please? Thank you! mills -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From nlinke at sbch.org Tue Apr 19 18:03:49 2016 From: nlinke at sbch.org (Noelle Linke) Date: Tue, 19 Apr 2016 23:03:49 +0000 Subject: [Histonet] outsourcing transcription Message-ID: Hi all, Does anyone outsource their transcription work? If so, do you have a service that you recommend? Thank you, No?lle No?lle Linke, MS, HTL(ASCP) QIHC Manager, Anatomic Pathology Pacific Diagnostic Laboratories nlinke at sbch.org Phone: (805) 324-9814 Fax: (805) 696-6433 ________________________________ CH Disclaimer: This electronic mail message is intended exclusively for the individual or entity to which it is addressed. This message, together with any attachment, may contain confidential and privileged information. Any views, opinions or conclusions expressed in this message are those of the individual sender and do not necessarily reflect the views of Cottage Health, its subsidiaries or affiliates. This document may also contain information covered under the Health Insurance Portability and Accountability Act (HIPAA, PL 104-191) and implementing regulations and must be protected in accordance with those provisions. Re-disclosure without patient consent or as otherwise permitted by law is prohibited. Any unauthorized review, retransmission, use, printing, copying, retention, disclosure, distribution or the taking of any action in reliance upon this information by persons or entities other than the intended recipient is strictly prohibited and may be unlawful. If you have received this message in error, please immediately advise the sender by reply email message to the sender and delete all copies of this message from your system without copying. ________________________________ From tratroyer at gmail.com Wed Apr 20 08:18:04 2016 From: tratroyer at gmail.com (Travis Troyer) Date: Wed, 20 Apr 2016 08:18:04 -0500 Subject: [Histonet] Frozen Sections Message-ID: I have a question that I wanted some input on. We recently did a frozen section and the patient was confirmed hepatitis B. I was wondering if anyone had any protocols for the stains and the cleanliness of the slides after the frozen section was retrieved. This is this the first time this came up and we were wondering about viral contamination in the stains and the safety of employees beyond the cryostat. I have found information on cleaning the cryostat, but I was wondering if anyone did any cleaning of the staining table after the slides with contaminated tissue were stained. Thanks, Travis Troyer From relia1 at earthlink.net Wed Apr 20 10:21:51 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 20 Apr 2016 11:21:51 -0400 Subject: [Histonet] Next Week is National Laboratory Professionals Week April 24-30, 2016 Message-ID: <1c7e01d19b18$f34b1700$d9e14500$@earthlink.net> Hello Megan, Next week 4/24-4/30 is National Medical Laboratory Professionals Week. Many labs are celebrating with trivia contests, luncheons, themed days and general activities to promote their professions. As you might already know I publish a monthly newsletter to my subscriber list of over 1800 histology professionals listing all of the current openings I am working on. I will be publishing a special lab week edition next week and was wondering if you had any current or future histology openings I can assist you with and include in my newsletter. This is not a solicitation for advertising. I am a recruiter who specializes in the permanent placement of histology professionals. I only inform my subscribers about positions with clients I am working with. I can help you find the next member of your histology team. Let me save you time and money in your search. Put me and my resources to work for you. If you would like more information I can send you another e-mail containing our fee agreement and additional information about our services. And I would love to have the opportunity to assist you with and add any current or future histology openings to my Lab Week Newsletter. Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From relia1 at earthlink.net Wed Apr 20 10:34:32 2016 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 20 Apr 2016 11:34:32 -0400 Subject: [Histonet] Histonet posting Next Week is National Laboratory Professionals Week April 24-30, 2016 PLEASE DISREGARD. Email was sent in error Message-ID: <069201d19b1a$24c6ac30$6e540490$@earthlink.net> Hi Histonetters, Please disregard my last posting it was sent in error. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From sbaldwin at mhhcc.org Wed Apr 20 10:55:24 2016 From: sbaldwin at mhhcc.org (Baldwin, Kathy) Date: Wed, 20 Apr 2016 15:55:24 +0000 Subject: [Histonet] alcohol or formalin filtration system Message-ID: Hi Histonetters Is anyone currently using the alcohol or formalin filtration systems and if you are can I have your thoughts?? Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From liz at premierlab.com Wed Apr 20 11:29:15 2016 From: liz at premierlab.com (Elizabeth Chlipala) Date: Wed, 20 Apr 2016 10:29:15 -0600 Subject: [Histonet] alcohol or formalin filtration system In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02BEC2AD5DDA@SBS2K8.premierlab.local> We have used the filtration systems in the past - the one thing you need to keep in mind is that the percentage of alcohol you put in is the percentage you get out. You need to test with a hydrometer and account for temperature what you get out. So you only want to put in your absolute and possibly your 95% if you put in 70% or 80% at the same time you will decrease your concentration too much. We use a fractional distillation unit now and even with that we get out ranges from 90 to 95% for alcohol when we account for temperature when using a hydrometer. We are also able to recycle our xylene, and propar with the same unit so it has helped us out a lot with both the amount of waste we generate and especially the amount of xylene that we need to order. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Baldwin, Kathy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 20, 2016 9:55 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] alcohol or formalin filtration system Hi Histonetters Is anyone currently using the alcohol or formalin filtration systems and if you are can I have your thoughts?? Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang at gmx.at Wed Apr 20 11:41:37 2016 From: gu.lang at gmx.at (Gudrun Lang) Date: Wed, 20 Apr 2016 18:41:37 +0200 Subject: [Histonet] copper stain Message-ID: <000801d19b23$82bf49b0$883ddd10$@gmx.at> Hi all! Which stain would you prefer to demonstrate copper? Rhodanin or Victoria blue? Thanks in advance Gudrun From rjbuesa at yahoo.com Wed Apr 20 12:08:21 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 20 Apr 2016 17:08:21 +0000 (UTC) Subject: [Histonet] copper stain In-Reply-To: <000801d19b23$82bf49b0$883ddd10$@gmx.at> References: <000801d19b23$82bf49b0$883ddd10$@gmx.at> Message-ID: <1781168460.3447638.1461172101718.JavaMail.yahoo@mail.yahoo.com> Timm's silver stain always!Ren? On Wednesday, April 20, 2016 12:51 PM, Gudrun Lang via Histonet wrote: Hi all! Which stain would you prefer to demonstrate copper? Rhodanin or Victoria blue? Thanks in advance Gudrun _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Wed Apr 20 13:56:56 2016 From: patpxs at gmail.com (Paula Sicurello) Date: Wed, 20 Apr 2016 18:56:56 +0000 (UTC) Subject: [Histonet] VIP 6 owners manual References: <1809881544.3419423.1461178616018.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1809881544.3419423.1461178616018.JavaMail.yahoo@mail.yahoo.com> Hello Netters, Does anyone have an electronic copy of the owners manual for a Tissue Tek VIP6?? If you do can you send it to me? Sakura charges $129 for one!! Thanks, Paula UC San Diego Health From tratroyer at gmail.com Wed Apr 20 14:32:06 2016 From: tratroyer at gmail.com (Travis Troyer) Date: Wed, 20 Apr 2016 14:32:06 -0500 Subject: [Histonet] viral contamination during frozen sections Message-ID: I have a question that I wanted some input on. Our lab was thinking about the possibilities a patient with a viral infection having a frozen section (hepatitis, HIV, etc.) . I was wondering if anyone had any protocols for the stains and the cleanliness of the slides after the frozen section was retrieved. This is this the first time this thought came up and we were wondering about viral contamination in the stains and the safety of employees beyond the cryostat. I have found information on cleaning the cryostat, but I was wondering if anyone did any cleaning of the staining table after the slides with contaminated tissue were stained. Thanks, Travis Troyer From Timothy.Morken at ucsf.edu Wed Apr 20 16:39:11 2016 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 20 Apr 2016 21:39:11 +0000 Subject: [Histonet] viral contamination during frozen sections In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF6FD3CC22@ex07.net.ucsf.edu> Travis, check out the CDC website http://www.cdc.gov/hicpac/Disinfection_Sterilization/6_0disinfection.html Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Travis Troyer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 20, 2016 12:32 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] viral contamination during frozen sections I have a question that I wanted some input on. Our lab was thinking about the possibilities a patient with a viral infection having a frozen section (hepatitis, HIV, etc.) . I was wondering if anyone had any protocols for the stains and the cleanliness of the slides after the frozen section was retrieved. This is this the first time this thought came up and we were wondering about viral contamination in the stains and the safety of employees beyond the cryostat. I have found information on cleaning the cryostat, but I was wondering if anyone did any cleaning of the staining table after the slides with contaminated tissue were stained. Thanks, Travis Troyer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ahp0878 at yahoo.com Thu Apr 21 01:26:55 2016 From: ahp0878 at yahoo.com (andrea price) Date: Wed, 20 Apr 2016 23:26:55 -0700 Subject: [Histonet] Histology Technologist Position / shift differential / south Georgia Message-ID: <0AA897BD-146B-4523-9B41-985EF67727C0@yahoo.com> Hi my name is Andrea Price I would like to submit my resume for review of this position should I contact you or Matt Hawes? Sent from my iPhone From davidkemlerllc at msn.com Thu Apr 21 12:12:28 2016 From: davidkemlerllc at msn.com (David) Date: Thu, 21 Apr 2016 13:12:28 -0400 Subject: [Histonet] Frozen section practice tissue? Message-ID: What seems to work best, other than human tissue, to practice FS's and Mohs? Dave From gu.lang at gmx.at Thu Apr 21 11:57:32 2016 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 21 Apr 2016 18:57:32 +0200 Subject: [Histonet] WG: copper stain In-Reply-To: <000801d19b23$82bf49b0$883ddd10$@gmx.at> References: <000801d19b23$82bf49b0$883ddd10$@gmx.at> Message-ID: <001501d19bee$e7503490$b5f09db0$@gmx.at> Thank you all for your responses. Gudrun -----Urspr?ngliche Nachricht----- Von: Gudrun Lang via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 20. April 2016 18:42 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] copper stain Hi all! Which stain would you prefer to demonstrate copper? Rhodanin or Victoria blue? Thanks in advance Gudrun _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cdemarinis at SARATOGACARE.ORG Thu Apr 21 12:57:37 2016 From: cdemarinis at SARATOGACARE.ORG (Demarinis, Carolyn) Date: Thu, 21 Apr 2016 17:57:37 +0000 Subject: [Histonet] Markers to label tissue cassettes and slides Message-ID: <1C75A843982A7B44BB368A3CC946ABCA012D60403A@SHEXCHMBX01.SARAHOSP.ORG> We are having problems with the markers we use, tried several brands, but they either smudge or are difficult to read after processing. Any recommendations? Thank you. -- This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy at saratogacare.org and destroy all copies of this communication and any attachments. From alkhenaizik at gmail.com Thu Apr 21 13:38:05 2016 From: alkhenaizik at gmail.com (kalkhenaizi .) Date: Thu, 21 Apr 2016 21:38:05 +0300 Subject: [Histonet] SV40 control Message-ID: Dear Histonetters, I am looking for SV40 control tissue. I would appreciate if you can share or exchange with my control blocks. Regards, Kadhem Lab Manager ExpressMed Labs Bahrain From leonard at rrclinicallab.com Thu Apr 21 21:25:59 2016 From: leonard at rrclinicallab.com (leonard at rrclinicallab.com) Date: Thu, 21 Apr 2016 21:25:59 -0500 Subject: [Histonet] SV40 control Message-ID: <16c1b6136535a37f721e3ae6f9a9cbec@rrclinicallab.com> Hi, We get our from a small lab.If your interested call Len at 708.783.7441. Macneal Hospital 3235 Berwyn Il, Supervisor From boneslides at aol.com Fri Apr 22 00:05:28 2016 From: boneslides at aol.com (boneslides at aol.com) Date: Fri, 22 Apr 2016 01:05:28 -0400 Subject: [Histonet] hello friend Message-ID: <1543c5bc4ef-4885-e296@webprd-a56.mail.aol.com> http://besthomes4u.com/hope-043911.php From: boneslides Code: bgyvz5vv32bghcz3c3 Time: 4/22/2016 6:05:27 AM From ashnews at comcast.net Fri Apr 22 06:12:57 2016 From: ashnews at comcast.net (ashnews at comcast.net) Date: Fri, 22 Apr 2016 11:12:57 +0000 (UTC) Subject: [Histonet] Missouri/Arkansas Joint Spring Meeting in Branson MO In-Reply-To: <1984016294.5996056.1460039957619.JavaMail.zimbra@comcast.net> Message-ID: <855410017.16552268.1461323577156.JavaMail.zimbra@comcast.net> Want something interesting to do May 19-21, 2016? Attend the Missouri/Arkansas Histotechnology Annual Symposium in Branson, MO It's a beautiful time of year to see Branson! * ?Earn 13 CEU's to apply to your ASCP CMP Program! * ?Browse the 16 + exhibitors, network & see all the new products. * ?Attend the HT Readiness Workshop all day Saturday and be entered in a drawing for a $500.00 Educational Scholarship sponsored by StatLab! * ?Visit the NSH Booth see what's new and be entered into a drawing for a free membership NSH! * Stop in and enjoy Ada Feldman's "The Joy of Cooking" lecture on Thursday evening and then on to the Vendor Reception! * Enjoy a complimentary luncheon/presentation on Friday presented by Myriad Genetics. We would love to see you! ? ? If you are coming but are awaiting funds just return your registration form so we know your coming!???? ? Contact either of us for a program or further information and it is not limited to MO/AR - everyone id welcome for all of our surrounding states!! ? Sharon Walsh, HT(ASCP),QIHC, President Missouri Society for Histotechnology 20 Brethold Drive Fenton, MO 63026 314-941-2301 ? Pamela Marcum Arkansas Society for Histotechnology 2315 Raintree Dr Bryant AR 72022 501-553-2224 From tammy_weiss at fws.gov Fri Apr 22 08:58:10 2016 From: tammy_weiss at fws.gov (Weiss, Tammy) Date: Fri, 22 Apr 2016 07:58:10 -0600 Subject: [Histonet] Markers to label tissue cassettes and slides In-Reply-To: <1C75A843982A7B44BB368A3CC946ABCA012D60403A@SHEXCHMBX01.SARAHOSP.ORG> References: <1C75A843982A7B44BB368A3CC946ABCA012D60403A@SHEXCHMBX01.SARAHOSP.ORG> Message-ID: Hi Carolyn, Our facility currently uses *VWR Chemically Resistant Markers (Cat.# 95042-566)*. I have tried numerous markers over the last 15-20 years and have found these markers to be the most consistant, smudge-free and remain dark after the staining procedure. Oddly enough, certain markers appear to stay on better with different cassettes and different clearants. I have found that if I change to a different cassette, I often have to change markers. Our facility uses Propar Clearant from Anatech Ltd (we don't use Xylene as the clearant) and Simport Histosette 1 (#M490-3). Hope this helps! Tammy Tammy Weiss Fish Biologist-Histology Dept. Bozeman Fish Health Center USFWS- Dept. of Interior tammy_weiss at fws.go v On Thu, Apr 21, 2016 at 11:57 AM, Demarinis, Carolyn via Histonet < histonet at lists.utsouthwestern.edu> wrote: > We are having problems with the markers we use, tried several brands, but > they either smudge or are difficult to read after processing. Any > recommendations? Thank you. > > > -- > This e-mail communication and any attachments may contain > confidential and privileged information for the use of the > designated recipients named above. > If you are not the intended recipient, you are hereby notified > that you have received this communication in error and that > any review, disclosure, dissemination, distribution or copying > of it or its contents is prohibited. If you have received this > communication in error, please notify Saratoga Hospital > immediately by e-mail at privacy at saratogacare.org and > destroy all copies of this communication and any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ihcworkshop at gmail.com Fri Apr 22 10:00:37 2016 From: ihcworkshop at gmail.com (Ihc Workshop) Date: Fri, 22 Apr 2016 08:00:37 -0700 Subject: [Histonet] IHC Hands-On Wet Workshop San Francisco Bay Area, 12 CEU Message-ID: <3B3DB4FB-F4A8-4029-80F3-4178756184AF@gmail.com> A small group IHC wet workshop in hands-on training of human, animal and mouse tissue staining, each attendee receives slides to stain .......Course is taught in an equipped lab; Few spots left. For details contact Maria at Ihcworkshop at gmail.com From ktuttle at umm.edu Fri Apr 22 12:47:31 2016 From: ktuttle at umm.edu (Tuttle, Kimberly) Date: Fri, 22 Apr 2016 17:47:31 +0000 Subject: [Histonet] Travelling histotechs Message-ID: <2BA60EB4F7D1C846BBF2B7AFB517CA152E72C4F5@UMMSTOCEXC04.umms.umm.edu> I've been in Histology for 21 years and now that my kids are practically adults, I think I would enjoy being a travelling histotech. I would like to hear from some people who work in this type environment about the pros and cons. Kimberly This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure. State and federal law protect the confidentiality of this information. If the reader of this message is not the intended recipient; you are prohibited from using, disclosing, reproducing or distributing this information; you should immediately notify the sender by telephone or e-mail and delete this e-mail. From wbenton at cua.md Mon Apr 25 16:49:34 2016 From: wbenton at cua.md (Walter Benton) Date: Mon, 25 Apr 2016 21:49:34 +0000 Subject: [Histonet] Position Message-ID: https://careers-wmich.icims.com/jobs/1096/lead-histotechnologist/job ? CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From calhouan at ohsu.edu Mon Apr 25 17:22:32 2016 From: calhouan at ohsu.edu (Andrea Calhoun) Date: Mon, 25 Apr 2016 22:22:32 +0000 Subject: [Histonet] Using same DAB for multiple slide racks Message-ID: <5030A8B38F932048A3DECBBA52ECB05C86AABA5F@EXMB11.ohsu.edu> Hi Listers! Has anyone ever tried re-using DAB? Not like week or day old DAB, but using the same DAB solution for more than one rack of slides? Anyone know the length of time DAB is activated for before becoming inactivated, or quenched? Safe to do, if more H202 is added? After antibody validation, we typically know how long to stain with DAB so we can stain a huge batch (24slides/rack, normally 2-3 racks) each in their own 150mls of Dab solution. But the DAB tablets we get are expensive and we are seeing an increase in the amount of IHC runs we're doing- so the whole thing is becoming expensive. If it is viable to use the same 150mls of DAB for 2-3 racks of slides, that would greatly decrease the amount we're using and cost. If anyone has ever tried this, or does this routinely- please let me know! Andrea Calhoun From blayjorge at gmail.com Mon Apr 25 19:22:06 2016 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Mon, 25 Apr 2016 20:22:06 -0400 Subject: [Histonet] Corona radiata (ovum): how early in animal evolution is there a corona radiata? Message-ID: Dear Histonetters: How early in animal evolution is there a corona radiata (layer encircling the ovum)? Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From rjbuesa at yahoo.com Tue Apr 26 07:34:33 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 26 Apr 2016 12:34:33 +0000 (UTC) Subject: [Histonet] Using same DAB for multiple slide racks In-Reply-To: <5030A8B38F932048A3DECBBA52ECB05C86AABA5F@EXMB11.ohsu.edu> References: <5030A8B38F932048A3DECBBA52ECB05C86AABA5F@EXMB11.ohsu.edu> Message-ID: <2034935950.1938691.1461674073936.JavaMail.yahoo@mail.yahoo.com> This is not a good practice and can lead to inconsistent results.Always use freshly prepared DAB sol. to finally be able to see the end product of the costly and important IHC procedure, it is worth it.Ren? On Monday, April 25, 2016 6:27 PM, Andrea Calhoun via Histonet wrote: Hi Listers! Has anyone ever tried re-using DAB? Not like week or day old DAB, but using the same DAB solution for more than one rack of slides? Anyone know the length of time DAB is activated for before becoming inactivated, or quenched?? Safe to do, if more H202 is added? After antibody validation, we typically know how long to stain with DAB so we can stain a huge batch (24slides/rack, normally 2-3 racks) each in their own 150mls of Dab solution. But the DAB tablets we get are expensive and we are seeing an increase in the amount of IHC runs we're doing- so the whole thing is becoming expensive.? If it is viable to use the same 150mls of DAB for 2-3 racks of slides, that would greatly decrease the amount we're using and cost. If anyone has ever tried this, or does this routinely- please let me know! Andrea Calhoun _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LaurenHegnerSweeney at uga.edu Tue Apr 26 07:50:29 2016 From: LaurenHegnerSweeney at uga.edu (Lauren Sweeney) Date: Tue, 26 Apr 2016 12:50:29 +0000 Subject: [Histonet] Sakura's Histo-Tek SL Slide Stainer Message-ID: Hello all in Histo land, I was wondering if anyone out there has been using, or has used, Sakura's Histo-Tek SL Slide Stainer? It is for low- volume labs. We are thinking about getting one and getting some reviews on it would be great. Thanks, Lauren Lauren Sweeney Pathology Laboratory Manager University of Georgia | College of Veterinary Medicine Department of Population Health 953 College Station Rd | Athens, GA 30605 706-583-0636 | lmarie08 at uga.edu From patpxs at gmail.com Tue Apr 26 08:32:41 2016 From: patpxs at gmail.com (P Sicurello) Date: Tue, 26 Apr 2016 06:32:41 -0700 Subject: [Histonet] Thank you --VIP 6 Manual Message-ID: Thanks to all who sent me the VIP 6 manual. Now we have something we can thumb through! Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From patrick.lewis at seattlechildrens.org Tue Apr 26 11:30:28 2016 From: patrick.lewis at seattlechildrens.org (Lewis, Patrick) Date: Tue, 26 Apr 2016 16:30:28 +0000 Subject: [Histonet] I did it I am now a certified HTL Message-ID: <1b4bbbd01e194c1eb34a0329e9ea963f@PPWEXM01.childrens.sea.kids> Hi Everyone After years of putting it off, I finally took my ASCP HTL exam and passed it. Huzzah! Patrick. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tbraud at holyredeemer.com Tue Apr 26 12:41:21 2016 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 26 Apr 2016 17:41:21 +0000 Subject: [Histonet] Passing the HTL Message-ID: <48E053DDF6CE074DB6A7414BA05403F806FEA9@HRHEX02-HOS.holyredeemer.local> BIG Congratulations! To Patrick Lewis on passing your HTL Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 7. I did it I am now a certified HTL (Lewis, Patrick) Message: 7 Date: Tue, 26 Apr 2016 16:30:28 +0000 From: "Lewis, Patrick" Subject: [Histonet] I did it I am now a certified HTL Hi Everyone After years of putting it off, I finally took my ASCP HTL exam and passed it. Huzzah! Patrick. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 From wdesalvo.cac at outlook.com Tue Apr 26 13:39:18 2016 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Tue, 26 Apr 2016 11:39:18 -0700 Subject: [Histonet] Passing the HTL Message-ID: Congratulations! Well done and now the fun begins. Sent from my Windows Phone ________________________________ From: Terri Braud via Histonet Sent: ?4/?26/?2016 10:58 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Passing the HTL BIG Congratulations! To Patrick Lewis on passing your HTL Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 7. I did it I am now a certified HTL (Lewis, Patrick) Message: 7 Date: Tue, 26 Apr 2016 16:30:28 +0000 From: "Lewis, Patrick" Subject: [Histonet] I did it I am now a certified HTL Hi Everyone After years of putting it off, I finally took my ASCP HTL exam and passed it. Huzzah! Patrick. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doolee at shands.ufl.edu Tue Apr 26 15:15:54 2016 From: doolee at shands.ufl.edu (Dooley, Elaine) Date: Tue, 26 Apr 2016 20:15:54 +0000 Subject: [Histonet] MYC-Cish Message-ID: <6193C53146742E4586DD4838F47F79AE0EA4F7D0@MSXMB02.Shands.local> Dear Histonetters, I was just wondering what CPT codes do people use that are doing MYC-cish on the Ventana Ultra strainers? Elaine Dooley Shands Teaching Hospital Gainesville FL 32655 352-265-0111 ext 72117 From vcaldwell4232 at gmail.com Tue Apr 26 15:38:02 2016 From: vcaldwell4232 at gmail.com (Van Caldwell) Date: Tue, 26 Apr 2016 13:38:02 -0700 Subject: [Histonet] Position Available in Santa Clara California Message-ID: Date Posted: 25/Apr/16 Requisition: 2066078 Job Title: Immuno-Histo Chemistry Applications Specialist Description: Are you looking to take your lab experience in Immuno-Histo Chemistry to the next level? To utilize your expertise to support the needs of our diverse internal and external customers in an exciting, dynamic role. Whether you are still working the bench or have since moved to other responsibilities, this exciting opportunity with Agilent will afford you the opportunity to capitalize on your experience and share your knowledge! Be a part of our growing internal education team where your technical and scientific expertise can be leveraged. Agilent is a leader in life sciences, diagnostics and applied chemical markets. We provide laboratories worldwide with instruments, services, consumables, applications and expertise, enabling customers to gain the insights they seek. Agilent gives doctors a head start in the fight against cancer and other diseases. Our solutions help pathology laboratories deliver fast, accurate information and help medical professionals make more precise diagnoses so patients can receive the most effective therapies. As an Immuno-Histo Chemistry Applications Specialist, your responsibilities will include acting as a subject matter expert for our internal staff and external customers. Responsibilities would include the delivery of instruction, mentoring and support from both an educational and technical perspective; assisting in translating customer's educational requirements into single seminars, customized solutions or projects, which improve the effectiveness and intellectual capital for our customers. Will also work with customers and internal organizations to develop specifications, and design and conduct technical training courses for customers and/or employees. Other tasks/ responsibilities may include: ? Leading participants in classroom lectures and/or laboratory training sessions. ? Providing support through technical communication with internal staff, field based representatives, customers and end users. ? Assist in developing new and novel methods of teaching relevant industry topics. ? Utilizing internal systems to place orders for regents, tissue and other supplies necessary to perform tasks. Qualifications: ? 5+ years relevant experience including hands on laboratory experience and strong pathology, technical, and instrument background. ? Post Graduate, Masters Degree, Bachelors Degree and/or equivalent combination of education and experience. ? Lab based Cytotechnologist or Histotechnologist experience. ? ASCP certification preferred but not required. ? Out of box thinker who can provide alternative ways to communicate a process ? Ability to effectively interact with a diverse group of people including various skill levels, degrees of seniority, personality, appreciation for the topic, and ego. ? Prior training and/or experience in automation focused ImmunoHisto Chemistry and In Situ Hybridization markets; including special stains and hematoxylin and Eosin preferred. Agilent Technologies Inc. is an equal opportunity employer. Qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, disability or any other protected categories under all applicable laws. Company: Dako Business: Diagnostics and Genomics Group Job Category: Support / Service Job Sub-Category: Education Region: Americas Country or Area: United States State/Province: California Town/City: Santa Clara Shift: Day Job Job Type: Experienced Schedule: Full-time Travel Required: Occasional Interested candidates can send Cover letter and CV to the following address: sean.nasri at agilent.com From criley at dpspa.com Wed Apr 27 10:22:23 2016 From: criley at dpspa.com (Charles Riley) Date: Wed, 27 Apr 2016 11:22:23 -0400 Subject: [Histonet] Sea urchin Spines Message-ID: Does anyone have any tips on how to get a section of a sea urchin spine? I tried decal but that didn't soften it at all and I tried using nair. If anyone has any suggestions please let me know -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs From eddessa at emory.edu Wed Apr 27 14:13:44 2016 From: eddessa at emory.edu (Dessasau III, Evan) Date: Wed, 27 Apr 2016 19:13:44 +0000 Subject: [Histonet] Sea urchin Spines In-Reply-To: References: Message-ID: You need something that will soften crystal calcite. Interesting, I watch my sea urchin every morning, I keep a Pincushion Urchin in my reef tank -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 27, 2016 11:22 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Sea urchin Spines Does anyone have any tips on how to get a section of a sea urchin spine? I tried decal but that didn't soften it at all and I tried using nair. If anyone has any suggestions please let me know -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Jose.R.deGuzman at gunet.georgetown.edu Wed Apr 27 15:57:29 2016 From: Jose.R.deGuzman at gunet.georgetown.edu (deGuzman, Jose R) Date: Wed, 27 Apr 2016 16:57:29 -0400 Subject: [Histonet] Sea urchin Spines In-Reply-To: References: Message-ID: Have you tried urine or something similar? I know that urine dissolves the spine. Does anyone have any tips on how to get a section of a sea urchin spine? I tried decal but that didn't soften it at all and I tried using nair. If anyone has any suggestions please let me know -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs MedStar Health is a not-for-profit, integrated healthcare delivery system, the largest in Maryland and the Washington, D.C., region. Nationally recognized for clinical quality in heart, orthopaedics, cancer and GI. IMPORTANT: This e-mail (including any attachments) may contain information that is private, confidential, or protected by attorney-client or other privilege. If you received this e-mail in error, please delete it from your system without copying it and notify sender by reply e-mail, so that our records can be corrected. Thank you. Help conserve valuable resources - only print this email if necessary. From JMacDonald at mtsac.edu Wed Apr 27 16:20:26 2016 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Wed, 27 Apr 2016 14:20:26 -0700 Subject: [Histonet] Sea urchin Spines In-Reply-To: References: Message-ID: I'm told that it is the ammonia in urine that is the "active ingredient". Perhaps trying ammonia water? From: "deGuzman, Jose R via Histonet" To: "histonet at lists.utsouthwestern.edu" Date: 04/27/2016 01:58 PM Subject: Re: [Histonet] Sea urchin Spines Have you tried urine or something similar? I know that urine dissolves the spine. Does anyone have any tips on how to get a section of a sea urchin spine? I tried decal but that didn't soften it at all and I tried using nair. If anyone has any suggestions please let me know -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs MedStar Health is a not-for-profit, integrated healthcare delivery system, the largest in Maryland and the Washington, D.C., region. Nationally recognized for clinical quality in heart, orthopaedics, cancer and GI. IMPORTANT: This e-mail (including any attachments) may contain information that is private, confidential, or protected by attorney-client or other privilege. If you received this e-mail in error, please delete it from your system without copying it and notify sender by reply e-mail, so that our records can be corrected. Thank you. Help conserve valuable resources - only print this email if necessary. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ashnews at comcast.net Wed Apr 27 20:08:50 2016 From: ashnews at comcast.net (ashnews at comcast.net) Date: Thu, 28 Apr 2016 01:08:50 +0000 (UTC) Subject: [Histonet] MO/AR Joint Spring Meeting in Branson MO - May 19th to 21st In-Reply-To: <2129587405.20592246.1461805676115.JavaMail.zimbra@comcast.net> Message-ID: <734403690.20592618.1461805730289.JavaMail.zimbra@comcast.net> Want something interesting to do May 19-21, 2016? Attend the Missouri/Arkansas Histotechnology Annual Symposium in Branson, MO It's a beautiful time of year to see Branson! * ?Earn 13 CEU's to apply to your ASCP CMP Program! * ?Browse the 16 + exhibitors, network & see all the new products. * ?Attend the HT Readiness Workshop all day Saturday and be entered in a drawing for a $500.00 Educational Scholarship sponsored by StatLab! * ?Visit the NSH Booth see what's new and be entered into a drawing for a free membership NSH! * Stop in and enjoy Ada Feldman's "The Joy of Cooking" lecture on Thursday evening and then on to the Vendor Reception! * Enjoy a complimentary luncheon/presentation on Friday presented by Myriad Genetics. We would love to see you! ? ? If you are coming but are awaiting funds just return your registration form so we know your coming!???? ? Contact either of us for a program or further information and it is not limited to MO/AR - everyone id welcome for all of our surrounding states!! ? Sharon Walsh, HT(ASCP),QIHC, President Missouri Society for Histotechnology 20 Brethold Drive Fenton, MO 63026 314-941-2301 ? Pamela Marcum Arkansas Society for Histotechnology 2315 Raintree Dr Bryant AR 72022 501-553-2224 From LRaff at uropartners.com Thu Apr 28 08:39:16 2016 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 28 Apr 2016 13:39:16 +0000 Subject: [Histonet] Medical/health related post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF10D3C437@COLOEXCH01.uropartners.local> http://www.chicagonow.com/downsize-maybe/2016/04/running-mates-and-other-mates-fighting-trump-fighting-cancer/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From wdesalvo.cac at outlook.com Thu Apr 28 09:20:50 2016 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Thu, 28 Apr 2016 07:20:50 -0700 Subject: [Histonet] Medical/health related post Message-ID: Again, why the non-Histo post. Take this to another source. I do not understand why Dr. Raff has not been removed from this list serve. This is a valuable site for histotechnolgy related issues, please let us keep it that way. Sent from my Windows Phone ________________________________ From: Lester Raff MD via Histonet Sent: ?4/?28/?2016 6:59 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Medical/health related post http://www.chicagonow.com/downsize-maybe/2016/04/running-mates-and-other-mates-fighting-trump-fighting-cancer/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Thu Apr 28 10:32:27 2016 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 28 Apr 2016 11:32:27 -0400 Subject: [Histonet] Happy Lab Week from Pam Barker and RELIA Solutions. Message-ID: <00e601d1a163$2bc96c30$835c4490$@earthlink.net> Hi Histonetters! I hope you are doing well. I wanted to send a special bulletin wishing you a Happy National Medical Laboratory Professionals Week. I Hope Your Lab Has Been Having Some Fun, Informative & Special Events to Celebrate Because You Deserve It! I have heard of some really cool things that labs are doing to mark the occasion including luncheons, costume, trivia and photo contests and displays to educate others about what you do. If you get a chance check out the ASCP's website section for Lab week. Here is the link: http://www.ascp.org/labweek I would love to hear what you are doing in your lab to celebrate so if you have a second please shoot me back an e-mail and let me know. I also wanted to let you know about some exciting new opportunities I am working on: Histology Supervisor - Amarillo, TX Senior Histotechnologist - Virginia Beach, VA BS degree required. 15K Sign on Bonus!! Histology Tech - Louisville, KY Electron Microscopy Tech - Louisville, KY IHC Support Specialist - Los Angeles County, CA Dermpath Histotech - Kansas City, MO Histotech II - Flagstaff, AZ Histotechnician - Austin, TX Histology Tech - Tyler, TX Grossing Histotech - Chattanooga, TN Mohs Histotech - Bakersfield, CA All of my positions are full time permanent positions my clients offer excellent compensation, benefits and relocation assistance and or sign on bonuses. If you or anyone you know might be interested please contact me. I can be reached toll free at 866-607-3542 or at relia1 at earthlink.net Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Maxim_71 at mail.ru Thu Apr 28 12:59:01 2016 From: Maxim_71 at mail.ru (Maxim Peshkov) Date: Thu, 28 Apr 2016 20:59:01 +0300 Subject: [Histonet] Sea urchin Spines Message-ID: <107605493.20160428205901@mail.ru> Charles, sea urchin spicules are contain a calcit, one of many forms of CaCO3. Just use a soft decal agent, as 14% EDTA with pH 7.0. Check endpoint carefully with weight loss weight gain test. -- Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71 at mail.ru From emartinez2 at echd.org Thu Apr 28 13:21:34 2016 From: emartinez2 at echd.org (Estela Martinez) Date: Thu, 28 Apr 2016 18:21:34 +0000 Subject: [Histonet] Tissue Sample Quality Message-ID: Hello Everyone, Working on CAP, is anyone willing to share their Procedure for the New ANP.10038 Tissue Sample Quality with me? Do you guys log all of your reprocess and regross? Estela Martinez Histology Supervisor Medical Center Hospital Odessa, TX 79761 432-640-2348 emartinez2 at echd.org CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party without permission of original user and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From taynay143 at hotmail.com Thu Apr 28 14:06:42 2016 From: taynay143 at hotmail.com (Tyra Connor) Date: Thu, 28 Apr 2016 19:06:42 +0000 Subject: [Histonet] Medical/health related post In-Reply-To: References: Message-ID: I have been on this list for some time now, and I have seen Dr. Raff contribute very useful information to many posed questions. Recently this situation has shifted, with more posts concerning his blog than actual helpful responses. This is not the second or even third time this situation has been addressed in this forum. It's sad because I would not want to lose his voice in these discussions, but these days whenever I see any post from him at all, I immediately delete it. I feel at this point that his refusal to stop is a small form of harassment, like a telemarketer that refuses to stop calling even though you request that they do so. Be full of wonder, be wonderful!! > On Apr 28, 2016, at 10:48 AM, WILLIAM DESALVO via Histonet wrote: > > Again, why the non-Histo post. Take this to another source. I do not understand why Dr. Raff has not been removed from this list serve. This is a valuable site for histotechnolgy related issues, please let us keep it that way. > > Sent from my Windows Phone > ________________________________ > From: Lester Raff MD via Histonet > Sent: ?4/?28/?2016 6:59 AM > To: 'histonet at lists.utsouthwestern.edu' > Subject: [Histonet] Medical/health related post > > http://www.chicagonow.com/downsize-maybe/2016/04/running-mates-and-other-mates-fighting-trump-fighting-cancer/ > > Lester J. Raff, MD MBA > UroPartners > Medical Director Of Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, Il 60154 > Tel: 708-486-0076 > Fax: 708-492-0203 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmtaylor at thedoctorsclinic.com Thu Apr 28 14:38:11 2016 From: tmtaylor at thedoctorsclinic.com (Taylor, Toni) Date: Thu, 28 Apr 2016 19:38:11 +0000 Subject: [Histonet] sectioning sea urchin spine Message-ID: <7117b7fde08642ebab6c7315bd90feb5@TDCEXCH.tdc.com> uric acid decomposes the calcium structure. So if you use urine, make sure you test it for uric acid. From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 27, 2016 11:22 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Sea urchin Spines Does anyone have any tips on how to get a section of a sea urchin spine? I tried decal but that didn't soften it at all and I tried using nair. If anyone has any suggestions please let me know -- Charles Riley HT(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Thanks, Toni Taylor The Doctor's Clinic Histology Laboratory (360) 782-3432 tmtaylor at thedoctorsclinic.com ________________________________ NOTICE: This e-mail and the attachments hereto, if any, may contain privileged and/or confidential information. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, you are hereby notified that any examination, distribution or copying of this e-mail and the attachments hereto, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by email or telephone and permanently delete this e-mail and the attachments hereto, if any, and destroy any printout thereof. The Doctors Clinic, APC, Bremerton, WA 98310 (360) 782-3600 From llewllew at shaw.ca Thu Apr 28 14:51:00 2016 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Thu, 28 Apr 2016 12:51:00 -0700 Subject: [Histonet] Medical/health related post In-Reply-To: References: Message-ID: <572269A4.3090807@shaw.ca> I think it is rather unfair to accuse Dr. Raff of harassment or misusing this forum. I recall that when he first posted references to his blog he asked for the members' permission to do so, and he was given that permission. Rather than abusing the privilege of posting to Histonet, I think he showed distinct courtesy for asking before he did so, and remarkable restraint in not responding to the, sometimes rude, responses. If members have now changed their minds about giving permission to him to past references to his blog, then let that permission be withdrawn with the same courtesy as he showed when first asking for it. Incidentally, it takes me about 0.5 seconds to click delete on his posts. I do not read them since I am a Canadian and have no real interest in US health care experiences or problems. Bryan Llewellyn Tyra Connor via Histonet wrote: > I have been on this list for some time now, and I have seen Dr. Raff contribute very useful information to many posed questions. Recently this situation has shifted, with more posts concerning his blog than actual helpful responses. This is not the second or even third time this situation has been addressed in this forum. It's sad because I would not want to lose his voice in these discussions, but these days whenever I see any post from him at all, I immediately delete it. I feel at this point that his refusal to stop is a small form of harassment, like a telemarketer that refuses to stop calling even though you request that they do so. > > Be full of wonder, be wonderful!! > >> On Apr 28, 2016, at 10:48 AM, WILLIAM DESALVO via Histonet wrote: >> >> Again, why the non-Histo post. Take this to another source. I do not understand why Dr. Raff has not been removed from this list serve. This is a valuable site for histotechnolgy related issues, please let us keep it that way. >> >> Sent from my Windows Phone >> ________________________________ >> From: Lester Raff MD via Histonet >> Sent: ?4/?28/?2016 6:59 AM >> To: 'histonet at lists.utsouthwestern.edu' >> Subject: [Histonet] Medical/health related post >> >> http://www.chicagonow.com/downsize-maybe/2016/04/running-mates-and-other-mates-fighting-trump-fighting-cancer/ >> >> Lester J. Raff, MD MBA >> UroPartners >> Medical Director Of Laboratory >> 2225 Enterprise Dr. Suite 2511 >> Westchester, Il 60154 >> Tel: 708-486-0076 >> Fax: 708-492-0203 >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From KReif at stegh.on.ca Fri Apr 29 07:48:18 2016 From: KReif at stegh.on.ca (Kevin Reif) Date: Fri, 29 Apr 2016 12:48:18 +0000 Subject: [Histonet] Ventilation of formalin/xylol Message-ID: <62d8f35b68ad4fea9aaaf97b8d00aa4c@stegh.on.ca> Hi All: We are looking at designing a new Path Lab and the main issue I have is venting. We will have lots of square footage but we will be trying to vent formalin, xylol and alcohol fumes from the new location in the basement. In talking with engineers in in our previous reno the figure of 50 CFM of air flow is required to move formalin and my concern is that even with down draft venting we won't be able to move the formalin fumes up to the appropriate level ( 3 stories) to exhaust. If anyone has just gone through a reno or has information about what is working and what isn't it would be greatly appreciated. If you have pictures to share that would be a great bonus. Thanks in advance Kevin Reif- Senior Technologist Pathology- STEGH ________________________________ St. Thomas Elgin General Hospital 189 Elm St. St. Thomas Ontario N5R 5C4 (519) 631-2020 http://www.stegh.on.ca ________________________________ CONFIDENTIALITY NOTICE: This electronic communication and attached material is intended for the use of the individual or institution to which it is addressed and may not be distributed, copied or disclosed to any unauthorized persons. This communication may contain confidential or personal information that may be subject to the provisions of the Freedom of Information and Protection of Privacy Act or the Personal Health Information Protection Act. If you have received this communication in error, please return this communication to the sender and permanently delete the original and any copy of it from your computer system. Thank you for your co-operation and assistance. For more information on our Privacy Policy, please see our website at http://www.stegh.on.ca From rjbuesa at yahoo.com Fri Apr 29 10:17:03 2016 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 29 Apr 2016 15:17:03 +0000 (UTC) Subject: [Histonet] Medical/health related post In-Reply-To: <572269A4.3090807@shaw.ca> References: <572269A4.3090807@shaw.ca> Message-ID: <1531386490.3798997.1461943023823.JavaMail.yahoo@mail.yahoo.com> Bryan:1- who gave permission to Dr. Raff?2- how we know the permission was given?3- what percentage of HistoNet member gave the permission?4- why Dr. Raff is so stubborn to keep posting what ever he chooses in spite?of the rejection of probably more members than those who "gave him permission"?5- you write about some "rude" comments, how about?Dr. Raff's?arrogant disregard for the opinion of others?Ren? On Thursday, April 28, 2016 4:06 PM, Bryan Llewellyn via Histonet wrote: I think it is rather unfair to accuse Dr. Raff of harassment or misusing this forum. I recall that when he first posted references to his blog he asked for the members' permission to do so, and he was given that permission. Rather than abusing the privilege of posting to Histonet, I think he showed distinct courtesy for asking before he did so, and remarkable restraint in not responding to the, sometimes rude, responses. If members have now changed their minds about giving permission to him to past references to his blog, then let that permission be withdrawn with the same courtesy as he showed when first asking for it. Incidentally, it takes me about 0.5 seconds to click delete on his posts. I do not read them since I am a Canadian and have no real interest in US health care experiences or problems. Bryan Llewellyn Tyra Connor via Histonet wrote: > I have been on this list for some time now, and I have seen Dr. Raff contribute very useful information to many posed questions. Recently this situation has shifted, with more posts concerning his blog than actual helpful responses. This is not the second or even third time this situation has been addressed in this forum. It's sad because I would not want to lose his voice in these discussions, but these days whenever I see any post from him at all, I immediately delete it. I feel at this point that his refusal to stop is a small form of harassment, like a telemarketer that refuses to stop calling even though you request that they do so. > > Be full of wonder, be wonderful!! > >> On Apr 28, 2016, at 10:48 AM, WILLIAM DESALVO via Histonet wrote: >> >> Again, why the non-Histo post. Take this to another source. I do not understand why Dr. Raff has not been removed from this list serve. This is a valuable site for histotechnolgy related issues, please let us keep it that way. >> >> Sent from my Windows Phone >> ________________________________ >> From: Lester Raff MD via Histonet >> Sent: ?4/?28/?2016 6:59 AM >> To: 'histonet at lists.utsouthwestern.edu' >> Subject: [Histonet] Medical/health related post >> >> http://www.chicagonow.com/downsize-maybe/2016/04/running-mates-and-other-mates-fighting-trump-fighting-cancer/ >> >> Lester J. Raff, MD MBA >> UroPartners >> Medical Director Of Laboratory >> 2225 Enterprise Dr. Suite 2511 >> Westchester, Il 60154 >> Tel: 708-486-0076 >> Fax: 708-492-0203 >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hardin at oncology.wisc.edu Fri Apr 29 10:39:01 2016 From: hardin at oncology.wisc.edu (Joseph Hardin) Date: Fri, 29 Apr 2016 15:39:01 +0000 Subject: [Histonet] Medical/health related post In-Reply-To: <1531386490.3798997.1461943023823.JavaMail.yahoo@mail.yahoo.com> References: <572269A4.3090807@shaw.ca> <1531386490.3798997.1461943023823.JavaMail.yahoo@mail.yahoo.com> Message-ID: I agree, Rene. Dr. Raff has been warned numerous times to keep his posts professional and has continued to post spam. I give permission to the facilitator to remove him from the list. I find histonet to be a great resource and enjoy the professionalism of a majority of it's users. Joseph Hardin Senior Research Specialist UWCCC Experimental Pathology ________________________________________ From: Rene J Buesa via Histonet Sent: Friday, April 29, 2016 10:17:03 AM To: Bryan Llewellyn; Histonet Subject: Re: [Histonet] Medical/health related post Bryan:1- who gave permission to Dr. Raff?2- how we know the permission was given?3- what percentage of HistoNet member gave the permission?4- why Dr. Raff is so stubborn to keep posting what ever he chooses in spite of the rejection of probably more members than those who "gave him permission"?5- you write about some "rude" comments, how about Dr. Raff's arrogant disregard for the opinion of others?Ren? On Thursday, April 28, 2016 4:06 PM, Bryan Llewellyn via Histonet wrote: I think it is rather unfair to accuse Dr. Raff of harassment or misusing this forum. I recall that when he first posted references to his blog he asked for the members' permission to do so, and he was given that permission. Rather than abusing the privilege of posting to Histonet, I think he showed distinct courtesy for asking before he did so, and remarkable restraint in not responding to the, sometimes rude, responses. If members have now changed their minds about giving permission to him to past references to his blog, then let that permission be withdrawn with the same courtesy as he showed when first asking for it. Incidentally, it takes me about 0.5 seconds to click delete on his posts. I do not read them since I am a Canadian and have no real interest in US health care experiences or problems. Bryan Llewellyn Tyra Connor via Histonet wrote: > I have been on this list for some time now, and I have seen Dr. Raff contribute very useful information to many posed questions. Recently this situation has shifted, with more posts concerning his blog than actual helpful responses. This is not the second or even third time this situation has been addressed in this forum. It's sad because I would not want to lose his voice in these discussions, but these days whenever I see any post from him at all, I immediately delete it. I feel at this point that his refusal to stop is a small form of harassment, like a telemarketer that refuses to stop calling even though you request that they do so. > > Be full of wonder, be wonderful!! > >> On Apr 28, 2016, at 10:48 AM, WILLIAM DESALVO via Histonet wrote: >> >> Again, why the non-Histo post. Take this to another source. I do not understand why Dr. Raff has not been removed from this list serve. This is a valuable site for histotechnolgy related issues, please let us keep it that way. >> >> Sent from my Windows Phone >> ________________________________ >> From: Lester Raff MD via Histonet >> Sent: ?4/?28/?2016 6:59 AM >> To: 'histonet at lists.utsouthwestern.edu' >> Subject: [Histonet] Medical/health related post >> >> http://www.chicagonow.com/downsize-maybe/2016/04/running-mates-and-other-mates-fighting-trump-fighting-cancer/ >> >> Lester J. Raff, MD MBA >> UroPartners >> Medical Director Of Laboratory >> 2225 Enterprise Dr. Suite 2511 >> Westchester, Il 60154 >> Tel: 708-486-0076 >> Fax: 708-492-0203 >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From king.laurie at marshfieldclinic.org Fri Apr 29 10:41:48 2016 From: king.laurie at marshfieldclinic.org (King, Laurie J) Date: Fri, 29 Apr 2016 15:41:48 +0000 Subject: [Histonet] Medical/health related post In-Reply-To: References: <572269A4.3090807@shaw.ca> <1531386490.3798997.1461943023823.JavaMail.yahoo@mail.yahoo.com> Message-ID: <7578207839F50248A7A6CD33517295EA547F5161@MCL-EXMB03.mfldclin.org> Joe and Rene, you have singlehandedly removed me from the list. Don't take yourself so seriously, you will likely live longer. -----Original Message----- From: Joseph Hardin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, April 29, 2016 10:39 AM To: Rene J Buesa; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Medical/health related post I agree, Rene. Dr. Raff has been warned numerous times to keep his posts professional and has continued to post spam. I give permission to the facilitator to remove him from the list. I find histonet to be a great resource and enjoy the professionalism of a majority of it's users. Joseph Hardin Senior Research Specialist UWCCC Experimental Pathology ________________________________________ From: Rene J Buesa via Histonet Sent: Friday, April 29, 2016 10:17:03 AM To: Bryan Llewellyn; Histonet Subject: Re: [Histonet] Medical/health related post Bryan:1- who gave permission to Dr. Raff?2- how we know the permission was given?3- what percentage of HistoNet member gave the permission?4- why Dr. Raff is so stubborn to keep posting what ever he chooses in spite of the rejection of probably more members than those who "gave him permission"?5- you write about some "rude" comments, how about Dr. Raff's arrogant disregard for the opinion of others?Ren? On Thursday, April 28, 2016 4:06 PM, Bryan Llewellyn via Histonet wrote: I think it is rather unfair to accuse Dr. Raff of harassment or misusing this forum. I recall that when he first posted references to his blog he asked for the members' permission to do so, and he was given that permission. Rather than abusing the privilege of posting to Histonet, I think he showed distinct courtesy for asking before he did so, and remarkable restraint in not responding to the, sometimes rude, responses. If members have now changed their minds about giving permission to him to past references to his blog, then let that permission be withdrawn with the same courtesy as he showed when first asking for it. Incidentally, it takes me about 0.5 seconds to click delete on his posts. I do not read them since I am a Canadian and have no real interest in US health care experiences or problems. Bryan Llewellyn Tyra Connor via Histonet wrote: > I have been on this list for some time now, and I have seen Dr. Raff contribute very useful information to many posed questions. Recently this situation has shifted, with more posts concerning his blog than actual helpful responses. This is not the second or even third time this situation has been addressed in this forum. It's sad because I would not want to lose his voice in these discussions, but these days whenever I see any post from him at all, I immediately delete it. I feel at this point that his refusal to stop is a small form of harassment, like a telemarketer that refuses to stop calling even though you request that they do so. > > Be full of wonder, be wonderful!! > >> On Apr 28, 2016, at 10:48 AM, WILLIAM DESALVO via Histonet wrote: >> >> Again, why the non-Histo post. Take this to another source. I do not understand why Dr. Raff has not been removed from this list serve. This is a valuable site for histotechnolgy related issues, please let us keep it that way. >> >> Sent from my Windows Phone >> ________________________________ >> From: Lester Raff MD via >> Histonet >> Sent: ?4/?28/?2016 6:59 AM >> To: >> 'histonet at lists.utsouthwestern.edu'> ern.edu> >> Subject: [Histonet] Medical/health related post >> >> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.chicagonow.co >> m_downsize-2Dmaybe_2016_04_running-2Dmates-2Dand-2Dother-2Dmates-2Dfi >> ghting-2Dtrump-2Dfighting-2Dcancer_&d=BQIG_g&c=KNVzINr6WAqWApikNSnyDe >> Ou0ck0iFwcrMz92MxUhIs&r=p786LM3cJoAYt86AArdXtvmv4T85viNv8Kr7DQqueoo&m >> =zQYw7DTt6QO4ca6soddQsuQsgzTqsed0NR6LehFpEeQ&s=UZOGIyB_-zPePrA07j65R_ >> aeDWO5fV37QEY3B8ecFeM&e= >> >> Lester J. Raff, MD MBA >> UroPartners >> Medical Director Of Laboratory >> 2225 Enterprise Dr. Suite 2511 >> Westchester, Il 60154 >> Tel: 708-486-0076 >> Fax: 708-492-0203 >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwest >> ern.edu_mailman_listinfo_histonet&d=BQIG_g&c=KNVzINr6WAqWApikNSnyDeOu >> 0ck0iFwcrMz92MxUhIs&r=p786LM3cJoAYt86AArdXtvmv4T85viNv8Kr7DQqueoo&m=z >> QYw7DTt6QO4ca6soddQsuQsgzTqsed0NR6LehFpEeQ&s=vDpq67G9GhBuYLnGPGGwWzpd >> arPizFwyaVJJZdRsv5E&e= >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwest >> ern.edu_mailman_listinfo_histonet&d=BQIG_g&c=KNVzINr6WAqWApikNSnyDeOu >> 0ck0iFwcrMz92MxUhIs&r=p786LM3cJoAYt86AArdXtvmv4T85viNv8Kr7DQqueoo&m=z >> QYw7DTt6QO4ca6soddQsuQsgzTqsed0NR6LehFpEeQ&s=vDpq67G9GhBuYLnGPGGwWzpd >> arPizFwyaVJJZdRsv5E&e= > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=BQIG_g&c=KNVzINr6WAqWApikNSnyDeOu0c > k0iFwcrMz92MxUhIs&r=p786LM3cJoAYt86AArdXtvmv4T85viNv8Kr7DQqueoo&m=zQYw > 7DTt6QO4ca6soddQsuQsgzTqsed0NR6LehFpEeQ&s=vDpq67G9GhBuYLnGPGGwWzpdarPi > zFwyaVJJZdRsv5E&e= > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQIG_g&c=KNVzINr6WAqWApikNSnyDeOu0ck0iFwcrMz92MxUhIs&r=p786LM3cJoAYt86AArdXtvmv4T85viNv8Kr7DQqueoo&m=zQYw7DTt6QO4ca6soddQsuQsgzTqsed0NR6LehFpEeQ&s=vDpq67G9GhBuYLnGPGGwWzpdarPizFwyaVJJZdRsv5E&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQIG_g&c=KNVzINr6WAqWApikNSnyDeOu0ck0iFwcrMz92MxUhIs&r=p786LM3cJoAYt86AArdXtvmv4T85viNv8Kr7DQqueoo&m=zQYw7DTt6QO4ca6soddQsuQsgzTqsed0NR6LehFpEeQ&s=vDpq67G9GhBuYLnGPGGwWzpdarPizFwyaVJJZdRsv5E&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQIG_g&c=KNVzINr6WAqWApikNSnyDeOu0ck0iFwcrMz92MxUhIs&r=p786LM3cJoAYt86AArdXtvmv4T85viNv8Kr7DQqueoo&m=zQYw7DTt6QO4ca6soddQsuQsgzTqsed0NR6LehFpEeQ&s=vDpq67G9GhBuYLnGPGGwWzpdarPizFwyaVJJZdRsv5E&e= From rmhickey88 at gmail.com Fri Apr 29 11:29:37 2016 From: rmhickey88 at gmail.com (Ryan Michael Hickey) Date: Fri, 29 Apr 2016 12:29:37 -0400 Subject: [Histonet] In need of part-time/PRN grossing or histology work in Houston, TX area Message-ID: Hello Histonet, I am a current graduate of NAACLS-accredited ASCP certification programs in histology and pathologists' assistant. I maintain HTL licensure (current, 3 years), current qualification in immunohistochemistry, and am eligible to sit for the PA board exam (planned for October, 2016). Serving as a pathologists' assistant at The University of Texas Health Science Center at Houston, grossing a variety of routine and complex specimens and instructing pathology residents on proper grossing technique.I have a little more than four years' experience in the anatomic pathology and research settings and am looking for some additional work (part-time, PRN) where I can use my skills and experience to contribute positively to the efficiency of a Houston-based, quality anatomic pathology service. I appreciate your consideration and the opportunity to reach out. Kind Regards, Ryan Hickey, MS, HTL(ASCP)QIHC