From mjones at metropath.com Tue Sep 1 08:11:57 2015 From: mjones at metropath.com (Michael Ann Jones) Date: Tue, 1 Sep 2015 13:11:57 +0000 Subject: [Histonet] Histology Workload Message-ID: We log all ?unbillable? work. It is by hand and I must tally that by hand each month, but it helps log what is really being done in our lab. I tally things like, Histogels, QC control work (slides, blocks, validations), QIP studies, PT work, send-outs (those do take time), and of course all of the deepers, stains, IHC, etc. We charge some clients a ?handling? fee when sending blocks out for further testing if our pathologists are not involved. You do need help! Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com On 8/31/15, 3:26 PM, "Cartun, Richard via Histonet" wrote: >Dear Amy: > >Some of the companies doing molecular testing will pay you a fee for >"specimen procurement/handling" since they realize the burden that these >requests put on us. I suggest calling the company(s) that you deal with >and see if you can work something out with them. And, you're absolutely >correct; we are being asked to do more work on specimens today, yet >reimbursements are going down. Not a good combination. Based on your >description of the work that you and your colleagues are doing, you have >a good case for additional staffing. It looks like you would benefit >from a lab assistant that could help with accessioning, billing, and >send-outs. > >Richard > >Richard W. Cartun, MS, PhD >Director, Histology & The Martin M. Berman, MD Immunopathology & >Morphologic Proteomics Laboratory >Director, Biospecimen Collection Programs >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 972-1596 >(860) 545-2204 Fax > >-----Original Message----- >From: Amy Self via Histonet [mailto:histonet at lists.utsouthwestern.edu] >Sent: Monday, August 31, 2015 4:41 PM >To: histonet at lists.utsouthwestern.edu >Subject: [Histonet] Histology Workload > >Happy Monday to Everyone, > >I have been trying[ to figure out how to justify additional help in >histology - and it's been hard. My facility staffs according to >billables. This by no means feels fair to anyone in this department. >You could have a specimen that will provide one billable CPT code but can >produce as many as 20 paraffin blocks. And now it seems like we are >getting many request for molecular test that require us to mail the >patients material out but we have no way of showing that we did this work >- we call this free work. We get no credit for time spent preparing and >packaging pathology material to send out to reference labs. Am I missing >any CPT codes that can be used to show that we in fact did something in >addition to routine pathology to this case. > >We have 1 histotech - 1 histotech/histology supervisor and one histology >assistant. >Our block load averages from 125 to 170 daily. >We also prep non-gyn cytologies. >Accession all specimens that come in that lab. >All mail-outs >The histotech/histology supervisor is responsible for all of the billing >as well as keeping up with new and old policies/ requirements for CAP/ >auditing billing and the list could go on. > >We are drowning in our own workload but don't know how to prove that the >help is needed. Any help - advice - suggestions anything will be >appreciated. How can I prove to upper management that we need more help >although the billables/productivity numbers say different? > >Thanks in advance, >Amy Self >Histology Lab Senior Tech >Lab >Tidelands Georgetown Memorial Hospital >606 Black River Road >Georgetown, SC 29440 >843-520-8711 >ASelf at tidelandshealth.org > >NOTE: > The information contained in this message may be privileged, >confidential and protected from disclosure. If the reader of this message >is not the intended recipient, or an employee or agent responsible for >delivering this message to the intended recipient, you are hereby >notified that any dissemination, distribution or copying of this >communication is strictly prohibited. If you have received this >communication in error, please notify us immediately by replying to this >message and deleting it from your computer. >Thank you. >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >This e-mail message, including any attachments, is for the sole use of >the intended recipient(s) and may contain confidential and privileged >information. Any unauthorized review, use, disclosure, or distribution is >prohibited. If you are not the intended recipient, or an employee or >agent responsible for delivering the message to the intended recipient, >please contact the sender by reply e-mail and destroy all copies of the >original message, including any attachments. > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Tue Sep 1 12:59:04 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 1 Sep 2015 17:59:04 +0000 Subject: [Histonet] Histology Workload In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F802C39A@HRHEX02-HOS.holyredeemer.local> Dear Amy - With 30+ years of experience of running an extremely tight ship, I can see how you would be drowning in work from the loads you are describing. I will email you separately, 2 articles concerning calculation and documentation of workload. One is from CAP in conjunction with NSH, and the other is authored by Rene Buesa. These should help. Additional, take the time to call and document the workload and staffing from similar sized institutions with similar billed tests. Look for tasks that you do that other institutions do not assign within the Histology Department, such as billing and send outs. Every little bit helps. Then, write up your case, and present it with a specific request for "X" number of additional hours at a (Histo-tech, ass't, or office) specified level. You need to be specific with your proof and your request for additional help. It's the only way to be heard. I hope this helps, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 2. Histology Workload (Amy Self) 3. Re: Histology Workload (Cartun, Richard) 4. Re: Histology Workload (Michael Ann Jones) Message: 2 Date: Mon, 31 Aug 2015 16:41:10 -0400 From: Amy Self Subject: [Histonet] Histology Workload Happy Monday to Everyone, I have been trying[ to figure out how to justify additional help in histology - and it's been hard. My facility staffs according to billables. This by no means feels fair to anyone in this department. You could have a specimen that will provide one billable CPT code but can produce as many as 20 paraffin blocks. And now it seems like we are getting many request for molecular test that require us to mail the patients material out but we have no way of showing that we did this work - we call this free work. We get no credit for time spent preparing and packaging pathology material to send out to reference labs. Am I missing any CPT codes that can be used to show that we in fact did something in addition to routine pathology to this case. We have 1 histotech - 1 histotech/histology supervisor and one histology assistant. Our block load averages from 125 to 170 daily. We also prep non-gyn cytologies. Accession all specimens that come in that lab. All mail-outs The histotech/histology supervisor is responsible for all of the billing as well as keeping up with new and old policies/ requirements for CAP/ auditing billing and the list could go on. We are drowning in our own workload but don't know how to prove that the help is needed. Any help - advice - suggestions anything will be appreciated. How can I prove to upper management that we need more help although the billables/productivity numbers say different? Thanks in advance, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org From akelley at path.wustl.edu Tue Sep 1 21:46:46 2015 From: akelley at path.wustl.edu (Kelley, Amanda) Date: Wed, 2 Sep 2015 02:46:46 +0000 Subject: [Histonet] Missouri Society for Histotechnology Lunch and Learn Message-ID: Sent from my iPhone The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. From akelley at path.wustl.edu Tue Sep 1 21:49:44 2015 From: akelley at path.wustl.edu (Kelley, Amanda) Date: Wed, 2 Sep 2015 02:49:44 +0000 Subject: [Histonet] Missouri Society for Histotechnology Lunch and Learn In-Reply-To: References: Message-ID: [X]?Missouri Society for Histotechnology **Present?s a Fall Lunch n? Learn** The Optimization of Fixation & Processing One of the most fundamentally critical elements of diagnostic histopathology is first the ability to suspend all cellular activity in tissue and prevent degradation, and secondly to process that specimen in a manner that facilitates subsequent steps such as microtomy and staining. The successful result of this is a microscopic image of cellular detail that most closely emulates the morphologic structure of disease as well as normal tissue. The concept of ?fluid transfer? will be emphasized as a key factor attributing to the success of fixation and processing. Innovative design in instrumentation will be reviewed to better understand how the industry has responded to the demand for more efficient methods. Benefits and selective use of rapid fixation/processing will be discussed along with methods of troubleshooting. (2 CEU?s) The Circle of Service The challenges of health care, particularly in the field of Histology, oftentimes makes it difficult to deliver quality service. This session focuses on the imperative that the Histologist must always remember; that we are an integral and critical part of the diagnostic process. This session will detail the value and wealth that they to the patient, and therefore that they must maintain the standard of 'Quality Care'. (2 CEU?s) Thank you Sakura Finetek, U.S.A. for your support. Speaker: H. Skip Brown, M. Div., HT (ASCP) When:?October 3rd, 2015 Hosted by: Mercy Hospital 100 Mercy Way Joplin, MO 64804 Registration: 8:30 Program Time: 9:00 am to 3:00 pm Lunch Provided Mercy Conference Room 1. Enter from hospital entrance on east side. Contact Sharon Walsh for details at userwalsh at sbcglobal.net [X] The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. From jpalmer at svi.edu.au Tue Sep 1 21:55:14 2015 From: jpalmer at svi.edu.au (Jason Palmer) Date: Wed, 2 Sep 2015 12:55:14 +1000 (EST) Subject: [Histonet] axolotl lymphatics and Okada article In-Reply-To: <7340cf00689d.55b76235@uwo.ca> Message-ID: <266804299.2712.1441162514241.JavaMail.root@zstore.medstv.unimelb.edu.au> Hi all, A few weeks back I posted a question about identification of lymphatics in the axolotl (see below). John Kiernan suggested that enzyme histochem on paraffin sections might be possible, as per an article by Okada et al in 1994. I have been trying to hunt down this article but so far to no avail. Does anyone have a copy they may be able to scan and send me? It is in a procedings supplement, Lymphology journal: Okada, E. (1994). An improved enzyme-histochemical method for identification of lymphatic capillaries on paraffin sections. Lymphology 27 , Suppl:732-735. Book title is: Progress in Lymphology XIV?Proceedings of the XIVth Congress in Washington DC, 1993, MH Witte, CL Witte (Eds.): Lymphology 27(Suppl): 1-893, 1994. Many thanks , Jason -- Jason Palmer Histology Laboratory Coordinator O'Brien Institute / St Vincent's Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4045 fax +61 3 9416 0926 email: jpalmer at svi.edu.au ----- Original Message ----- From: "John Kiernan" To: "Jason Palmer" , histonet at lists.utsouthwestern.edu Sent: Wednesday, 29 July, 2015 2:06:29 AM Subject: Re: [Histonet] axolotl lymphatics Instead of an antibody, you might consider enzyme activity histochemistry, which is much less expensive. Demonstration of 5-nucleotidase activity in the presence of levamisole detects lymphatic endothelium. Sections can also be stained for alkaline phosphatase activity in the endothelium of blood vessels. Here are a few references. Kato, S., Yasunaga, A. and Uchida, U. (1991). Enzyme-histochemical method for identification of lymphatic capillaries. Lymphology 24 :125-129. Ohkuma, M. (1994). Simultaneous double staining for the blood and lymphatic capillary. Lymphology 27 , Suppl:106-107. Okada, E. (1994). An improved enzyme-histochemical method for identification of lymphatic capillaries on paraffin sections. Lymphology 27 , Suppl:732-735. Ji, R.C. and Kato, S. (2003). Lymphatic network and lymphangiogenesis in the gastric wall. Journal of Histochemistry and Cytochemistry 51 :331-338. Needless to say, none of these relate to amphibian tissues! John Kiernan Anatomy, UWO, London, Canada = = = On 26/07/15, Jason Palmer via Histonet wrote: Hi all, I need to find an antibody that will label lymphatic endothelial cells in the axolotl. Does anybody have any experience or ideas? I have tried a couple of our anti-mouse and anti-human Abs for podoplanin and LYVE-1 but no cross-reactivity so far. I have no experience with staining of non-mammalian tissues - maybe an anti-frog Ab would cross react? Does anyone have experience with other amphibians? Thanks, Jason _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ahughes8 at ITS.JNJ.com Wed Sep 2 08:57:19 2015 From: ahughes8 at ITS.JNJ.com (Hughes, Anna [JRDUS]) Date: Wed, 2 Sep 2015 13:57:19 +0000 Subject: [Histonet] NFkB Message-ID: <1062C7BE2E82104EBB0231BDCBE87E2FA2C608@ITSUSRAGMDGE05.jnj.com> Hi HistoPeeps! Anyone out there have any IHC experience with NFkB on mouse tissue? Any antibody suggestions, good literature references, or protocols would be welcome. Thanks in advance for your help! Anna Hughes, HTL, QIHC Ahughes8 at its.jnj.com From blayjorge at gmail.com Wed Sep 2 09:29:41 2015 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Wed, 2 Sep 2015 10:29:41 -0400 Subject: [Histonet] Mindfulness in STEM Education? Message-ID: Hello Histonetters: As a favor to a colleague, I would like to know if you know of faculty members, departments, groups of departments, universities, etc. incorporating mindfulness [*"Mindfulness* is the intentional, accepting and non-judgmental focus of one's attention on the emotions, thoughts and sensations occurring in the present moment, which can be trained by meditational practices that are described in detail in the Buddhist tradition." ( https://en.wikipedia.org/wiki/*Mindfulness)]* practices in STEM (science, technology, engineering, and mathematics) education. If you have any constructive feedback, please kindly direct it to me: blayjorge at gmail.com Apologies for potential duplicate email. Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From Lacie.Algeo at providence.org Wed Sep 2 14:32:04 2015 From: Lacie.Algeo at providence.org (Algeo, Lacie A) Date: Wed, 2 Sep 2015 19:32:04 +0000 Subject: [Histonet] bone marrow billing Message-ID: <24C4B3C167E5694887AB594C7602CE3A03C01C06@WN35104.or.providence.org> Hi All, Are you allowed to bill an 88305 for both the aspirate and the biopsy on bone marrow cases? Thank you, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo at providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From fbozkurt at gmail.com Thu Sep 3 01:36:16 2015 From: fbozkurt at gmail.com (Mehmet Fatih BOZKURT) Date: Thu, 3 Sep 2015 09:36:16 +0300 Subject: [Histonet] NFkB In-Reply-To: <1062C7BE2E82104EBB0231BDCBE87E2FA2C608@ITSUSRAGMDGE05.jnj.com> References: <1062C7BE2E82104EBB0231BDCBE87E2FA2C608@ITSUSRAGMDGE05.jnj.com> Message-ID: Hello, Anti-NFkB p65, SC-109, Santa Cruz (1/50 dilution and HieR pH 6.0) is good working on rat tissues. On Wed, Sep 2, 2015 at 4:57 PM, Hughes, Anna [JRDUS] via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi HistoPeeps! > Anyone out there have any IHC experience with NFkB on mouse tissue? Any > antibody suggestions, good literature references, or protocols would be > welcome. > > Thanks in advance for your help! > Anna Hughes, HTL, QIHC > Ahughes8 at its.jnj.com > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03100, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 From tmcampbe at fmh.org Thu Sep 3 06:53:12 2015 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Thu, 3 Sep 2015 11:53:12 +0000 Subject: [Histonet] Temporary Histotech Needed Message-ID: Hello everyone, I was wondering if there is anyone out there that does temporary work in histo labs in the Frederick MD area. I run a small GI lab and I will be going on maternity leave in October and we would like someone to fill in. I know that there are temp agencies I can go through, but I thought I would try leaving the middle man out first because we are a small lab and are trying to save money and they can have more costs. I would need someone for 6 to 8 wks. The tech would have to accession, gross the biopsies and of course process, embed, cut and stain. I also do warthin starry and ABPAS by hand. There are on average 30 blocks a day and 10 specials a day. You do your own thing. Go at your own pace. Flexible time. No one bothers you. Just as long as the work gets done. If there is anyone in the area that is willing to do this, please email or call me! Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) From taylor at prometheushealthcare.com Thu Sep 3 11:02:09 2015 From: taylor at prometheushealthcare.com (Taylor Rinaldi) Date: Thu, 3 Sep 2015 12:02:09 -0400 Subject: [Histonet] Job opportunity in Colorado- Message-ID: <008b01d0e661$e3c1ced0$ab456c70$@prometheushealthcare.com> Hello all! My name is Taylor Rinaldi, Recruiting Manager at Prometheus healthcare. We specialize specifically in laboratory recruiting all over the United States for many hospitals and reference laboratories. We just received a new order for a great opportunity with a well-known hospital located in Colorado. We are recruiting for a strong Histotechnician/Grosser for their lab. This position is a dayshift, fulltime, permanent opportunity. ASCP certification preferred. If you may be interested in this or any of our other opportunities, please reach out to me for immediate consideration. Thank you in advance! Taylor Rinaldi Recruiting Manager Prometheus Healthcare Office (301) 693-9057 Taylor at prometheushealthcare.com From Lisa.Freeman at fda.hhs.gov Thu Sep 3 11:18:14 2015 From: Lisa.Freeman at fda.hhs.gov (Freeman, Lisa*) Date: Thu, 3 Sep 2015 16:18:14 +0000 Subject: [Histonet] Job Arkansas Message-ID: <0F928833A4CE744793039B781368BED023C2F4DD@FDSWP3313.fda.gov> TOXICOLOGIC PATHOLOGY ASSOCIATES, INC. (TPA) National Center for Toxicological Research Jefferson, AR 72079 CAREER OPPORTUNITIES Posting closes September 1, 2015 Toxicologic Pathology Associates, Inc (TPA) is seeking candidates for several open histotechnician positions. TPA provides pathology and pathology-related services to the Food and Drug Administration's National Center for Toxicological Research (NCTR) located in Jefferson, Arkansas. Current positions range from entry level laboratory technician who can execute basic laboratory procedures to more advanced histotechnician positions for individuals who have an HT or HTL (ASCP) certification and have highly developed cognitive skill sets and may be responsible for complete project performance for both large and small projects and are able to supervise the work of, and train lower level technicians. TPA provides a comprehensive benefits package to its employees and a competitive starting salary commensurate with experience, expertise and skill set. Employment of successful candidates will contingent upon receipt of a satisfactory drug screening report, obtaining the necessary government security clearance and successfully passing the required physical examination. Drug screening is provided by an off-site third party and is paid for by TPA. The annual physical examination is provided by an NCTR Occupational Medical Program and is at no cost to the employee. Interested candidates should submit their resume and contact information to: Dr. Kelly Davis TPA Laboratory Director National Center for Toxicological Research 3900 NCTR Rd. Jefferson, AR 72079 Office (870)543-7600 Kelly.davis at fda.hhs.gov From lblazek at digestivespecialists.com Thu Sep 3 11:26:45 2015 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Thu, 3 Sep 2015 12:26:45 -0400 Subject: [Histonet] Job opportunity in Colorado- In-Reply-To: <008b01d0e661$e3c1ced0$ab456c70$@prometheushealthcare.com> References: <008b01d0e661$e3c1ced0$ab456c70$@prometheushealthcare.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E39174A0DB385@IBMB7Exchange.digestivespecialists.com> I'm sorry, I can't resist. I guess it's just too close to vacation time... How strong do you have to be? -----Original Message----- From: Taylor Rinaldi via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 03, 2015 12:02 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Job opportunity in Colorado- Hello all! My name is Taylor Rinaldi, Recruiting Manager at Prometheus healthcare. We specialize specifically in laboratory recruiting all over the United States for many hospitals and reference laboratories. We just received a new order for a great opportunity with a well-known hospital located in Colorado. We are recruiting for a strong Histotechnician/Grosser for their lab. This position is a dayshift, fulltime, permanent opportunity. ASCP certification preferred. If you may be interested in this or any of our other opportunities, please reach out to me for immediate consideration. Thank you in advance! Taylor Rinaldi Recruiting Manager Prometheus Healthcare Office (301) 693-9057 Taylor at prometheushealthcare.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech at imagesbyhopper.com Thu Sep 3 11:55:11 2015 From: histotech at imagesbyhopper.com (histotech at imagesbyhopper.com) Date: Thu, 03 Sep 2015 12:55:11 -0400 Subject: [Histonet] CLIA regs question Message-ID: <7AA54CE5-6F8E-4256-B6BD-7DBB800F7369@imagesbyhopper.com> Hi Histonetters! Question: on the application for AHCA/CLIA there is a line about who is the "clinical consultant ". This is a non-waived lab. The director is a state licensed, board certified pathologist. The testing is high-complexity. Can the pathologist serve as the clinical consultant? Thanks! Michelle From tmcampbe at fmh.org Thu Sep 3 12:42:12 2015 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Thu, 3 Sep 2015 17:42:12 +0000 Subject: [Histonet] water on slides Message-ID: I've been having this problem for a month or so. It is very humid in my lab right now because of the weather here so I am very cautious about changing and rotating everything everyday. But I am having issues with water being in the first slide of the first run everyday! Its so weird! That is when the reagents are the most fresh. And its only the first slide. It it was a problem of water being in the reagents, wouldn't I see water in most of the slides? Its very bizarre. Anyone else have this problem??? Thanks! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) From melissa at alliedsearchpartners.com Thu Sep 3 14:28:09 2015 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Thu, 3 Sep 2015 19:28:09 +0000 Subject: [Histonet] Histotech Job in Southern California Message-ID: Hello, Almost the holiday weekend! I have an opening for a full time/direct hire histotech Tuesday-Saturday Noon-8:30pm in Santa Ana, CA Area. Looking for QA/QC skills, clinical histology experience and ASCP certification. Please contact me for more details. Thank you! Melissa Allied Search Partners 888-388-7571 x. 102 From epeters2 at gmu.edu Thu Sep 3 20:35:06 2015 From: epeters2 at gmu.edu (Esther C Peters) Date: Fri, 4 Sep 2015 01:35:06 +0000 Subject: [Histonet] For sale: Lab storage - Tissue-Tek flat tray cases Message-ID: Tissue-Tek slide storage tray case. Capacity is 800 slides (50 trays of 16 slides each). These are hard to find in the US - model #4023. Slides store flat. Have 12 brand new still in boxes. $180 each. (703)342-7804 or katcandu at ymail.com From nicole at dlcjax.com Fri Sep 4 08:37:31 2015 From: nicole at dlcjax.com (Nicole Tatum) Date: Fri, 4 Sep 2015 13:37:31 +0000 Subject: [Histonet] Collection station Message-ID: I need some help. What exactly constitutes a "collection station". We are opening a satellite office and we are a Derm POL with our main lab at a different location. We plan on performing surgeries and bx and bringing the specimens back to our main lab. Does this constitute a collection station since we will be bringing the specimens back to the main office? Nicole Tatum BSH, HT From PAMarcum at uams.edu Tue Sep 8 13:09:51 2015 From: PAMarcum at uams.edu (Marcum, Pamela A) Date: Tue, 8 Sep 2015 18:09:51 +0000 Subject: [Histonet] HT/HTL Positions in Little Rock Message-ID: Good Afternoon, We have two openings in our Histology Laboratory at the University of Arkansas for Medical Sciences in Little Rock. We have competitive pay and a sign on bonus for the right candidates. The positions are for bench Histologist with experience however; we would consider someone who has their registry and may be new to the field. The work is general Histology with special stains and no IHC. Little Rock is located in Central Arkansas and is a beautiful place for all outdoor sports or hobbies as well as a well-rounded small city. The cost of living is very affordable. Please to the UAMS website at uams.edu and look for the employment or jobs area. The positions are under Technical. If you have questions please e-mail me at this address: pamarcum at uams.edu Thank You!! Pamela Marcum Histology Supervisor UAMS AP Histology Dept. ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jerrysedgewick at gmail.com Tue Sep 8 17:03:55 2015 From: jerrysedgewick at gmail.com (J. Sedgewick) Date: Tue, 8 Sep 2015 17:03:55 -0500 Subject: [Histonet] =?utf-8?q?TOMORROW=3A_=E2=80=9C5_Common_Mistakes_when_?= =?utf-8?q?using_Photoshop_for_Science=3A_Tips=2C_Tricks_and_a_Case?= =?utf-8?b?IFN0dWR54oCd?= In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39174A0DB385@IBMB7Exchange.digestivespecialists.com> References: <008b01d0e661$e3c1ced0$ab456c70$@prometheushealthcare.com> <5A2BD13465E061429D6455C8D6B40E39174A0DB385@IBMB7Exchange.digestivespecialists.com> Message-ID: <5A9B0F04E5B74D5FA0A04562A1624E42@sedge> Images are a vital component of scientific research for evaluation and data analysis. Speakers at this complimentary webinar will share their wisdom and insight to help you avoid these 5 costly mistakes in image processing. Sign up now: http://bit.ly/1LgIMs1 Images are a vital component of scientific research for evaluation, data analysis, visualizing the impact of experimental parameters, and in medicine for assessing disease conditions. Regardless of the equipment price tag or level of automation, microscope systems generally fall short in providing images with consistent quality optimized for color, contrast and brightness. Adobe? Photoshop? or other image editing software is commonly used to correct for image insufficiencies, but consequences exist. Join our speakers, Jerry Sedgewick and Dr. Dawn Dawson, MD, as they review the common mistakes encountered when using Photoshop for science, offer tips to achieve consistency and high quality in your imaging process, and share their personal experience on how to avoid these common mistakes altogether. The last notice! Jerry From mjdessoye at commonwealthhealth.net Wed Sep 9 07:23:00 2015 From: mjdessoye at commonwealthhealth.net (Dessoye, Michael) Date: Wed, 9 Sep 2015 12:23:00 +0000 Subject: [Histonet] Cryostat vacuum Message-ID: Hello Histonet, Does anyone know where I can find a cryostat vacuum? I've used them in the past but can't seem to find a manufacturer. Thanks! Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From KSimeone at leavittmgt.com Wed Sep 9 12:31:05 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Wed, 9 Sep 2015 17:31:05 +0000 Subject: [Histonet] FULLTIME DAY SHIFT (first shift) POSITION DELRAY BCH FL In-Reply-To: <43944B1DBAAC2846B7B9D626B5F1233C4D2DCE8A@vm-email.leavittmgt.com> References: <43944B1DBAAC2846B7B9D626B5F1233C4D2DCE8A@vm-email.leavittmgt.com> Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C4D2DD341@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Beach, Florida dermatology laboratory. This is a permanent full time, DAYTIME SHIFT (40 hours) position with benefits (medical/401k/vacation). Annual salary in the $50k range. THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! PLEASE VISIT THIS LINK TO APPLY: https://advancedderm.applicantpro.com/jobs/240090.html *full time position Mon-Fri 8a-5:30p (start time may vary) *MUST be licensed as a FLORIDA HISTOTECHNICIAN OR HISTOTECHNOLOGIST (NO pending licensure pls) *PREFER experience but WILL TRAIN a knowledgeable, willing candidate *duties to include (but not limited to): grossing, microtomy, accessioning, embedding and general histology *must be self motivated, reliable and a team player Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From jpiche at wtbyhosp.org Wed Sep 9 13:25:10 2015 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Wed, 9 Sep 2015 18:25:10 +0000 Subject: [Histonet] Automatic coverslippers Message-ID: <631955447A364B45B9458D2905635110D9679FDF@WIN08-MBX-02.wtbyhosp.org> Hey Everyone, I've been tasked to collect information on automatic coverslippers........I was wondering what everyone else is using out there? Do you like them? Do they make your lives easier or more difficult? How do the slides hold up over time? We have a Leica Autostainer XL. Can it be retrofitted for an Autostainer? Thanks in advance>:) Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From wbenton at cua.md Wed Sep 9 14:05:08 2015 From: wbenton at cua.md (Walter Benton) Date: Wed, 9 Sep 2015 19:05:08 +0000 Subject: [Histonet] Automatic coverslippers In-Reply-To: <631955447A364B45B9458D2905635110D9679FDF@WIN08-MBX-02.wtbyhosp.org> References: <631955447A364B45B9458D2905635110D9679FDF@WIN08-MBX-02.wtbyhosp.org> Message-ID: <31e8fd479d5340b6a33b22df86f1504d@MAIL01.GCU-MD.local> Jessica, I have the same Leica stainer with the attached coverslipper. CV 5030. It uses glass coverslips in varying sizes (you choose) based on what works for your lab. If you have a large volume of work to coverslip each day this will definitely save you time. More importantly, the coverslipping will not vary, as it can from tech to tech. The slides can be dry in 24-48 hours completely, but that is determined more by the mountant type and volume being applied. Archival is fine with no problems since the glass system is not as particular as the tape from other vendors. ***Tape is faster though.*** As for common issues that you may experience if the unit is not cleaned and maintained properly. Broken coverglass can cause jams or even worse break slides. The mountant needle can bend if the brush is allowed to dry and becomes hard, which can then cause your slides to receive little to no mountant on them. The arm that picks up slides can get out alignment, but this can easily be fixed with a restart (re-initialization) of the coverslipper. As for removing the left facing side of the unit, I think that can be done to retrofit the instrument with the link system, but check with your Leica rep to confirm. Linking the two systems removes the need for constant interaction by techs loading and unloading racks throughout the day. The take home message to all of this is, keep it clean and it generally works great every day without issue. If you don't it will eventually cause you havoc. We are very happy with our unit. If you have any other questions, please don't hesitate to reach out to me. Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: Piche, Jessica via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, September 09, 2015 2:25 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Automatic coverslippers Hey Everyone, I've been tasked to collect information on automatic coverslippers........I was wondering what everyone else is using out there? Do you like them? Do they make your lives easier or more difficult? How do the slides hold up over time? We have a Leica Autostainer XL. Can it be retrofitted for an Autostainer? Thanks in advance>:) Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From thisisann at aol.com Thu Sep 10 11:25:05 2015 From: thisisann at aol.com (Ann Specian) Date: Thu, 10 Sep 2015 12:25:05 -0400 Subject: [Histonet] Cassette Printers Message-ID: <14fb813222d-c2e-2c0e2@webprd-m86.mail.aol.com> I am looking to purchase a cassette printer. Does anyone have any suggestions as to which are the best and which should be avoided? Thanks, Ann From Lesley.Bechtold at jax.org Thu Sep 10 12:37:34 2015 From: Lesley.Bechtold at jax.org (Lesley Bechtold) Date: Thu, 10 Sep 2015 17:37:34 +0000 Subject: [Histonet] Histology Benchmarking Survey Message-ID: Dear Histonetters, We are conducting our biennial bench marking survey and we hope that you will participate. We have a created a short set of survey questions in the link below. Everyone who participates will receive a copy of the final data (de-identified). The data provides a useful snapshot of what other histological laboratories are doing, how you compare and it can be used as a budgeting tool for new equipment, new staff and so forth. Thank you in advance for completing our survey. The survey will close on September 30th and you will receive the final results in October. Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor ME 04609 207-288-6322 https://www.surveymonkey.com/r/HistologyBenchmarkingSurvey2015 The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From Pat.Patterson at propath.com Fri Sep 11 09:07:52 2015 From: Pat.Patterson at propath.com (Pat Patterson) Date: Fri, 11 Sep 2015 14:07:52 +0000 Subject: [Histonet] Dallas HT Position in IHC Lab Message-ID: <6DCB8B92D0138244B56CE8EACE0D458D2E77833B@Mail.propathlab.com> HISTOTECHNICIAN (IMMUNOHISTOCHEMISTRY DEPARTMENT) ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Histology Technician for its' Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. The ideal candidate will have a minimum of 4 years Histology experience with paraffin microtomy with a variety of different tissue types. Working knowledge of IHC theory required, hands on IHC performance is desired. We will train the ideal candidate to perform our manual IHC system. HT (ASCP) strongly desired. The hours for the position are 4:00 p.m. to 12:30 a.m. Monday through Friday. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EEO/AA-M/F/disability/protected veteran status Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to accessibility at propath.com. Pat Patterson, HTL(ASCP) Manager, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From srishan at mail.holyname.org Fri Sep 11 09:37:21 2015 From: srishan at mail.holyname.org (srishan at mail.holyname.org) Date: Fri, 11 Sep 2015 10:37:21 -0400 Subject: [Histonet] DISPOSABLE STERILE FORCEPS Message-ID: Hi, Does anyone know of a vendor who supplies sterile, individually wrapped , plastic forceps. Thanks for the input. Nirmala Srishan Histology Supervisor Holy Name Medical Center 718 Teaneck Road Teaneck NJ 07666 Lab: 201 833 3023 Office: 201 541 6328 Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From anna.coffey at nih.gov Fri Sep 11 11:54:24 2015 From: anna.coffey at nih.gov (Coffey, Anna (NIH/NCI) [C]) Date: Fri, 11 Sep 2015 16:54:24 +0000 Subject: [Histonet] Studying for ASCP qIHC Message-ID: <5C3E10119A1B824FBE92B08279F74A91025B2ACC@msgb10.nih.gov> Hi everyone, I've been studying for my qIHC exam at the end of this month and I bought the Michigan Society's study workbook which has been a very helpful study guide. It's my understanding that there is no accompanying study guide for this...is that right? If that's not true, I'd love to know where I can pick one up! If it is true, is there anyone (or a group) out there with an interest in developing one? I'd love to work on this with some folks if anyone is interested! Best, Anna From anna.coffey at nih.gov Fri Sep 11 12:54:46 2015 From: anna.coffey at nih.gov (Coffey, Anna (NIH/NCI) [C]) Date: Fri, 11 Sep 2015 17:54:46 +0000 Subject: [Histonet] Studying for ASCP qIHC Message-ID: <5C3E10119A1B824FBE92B08279F74A91025B2B0D@msgb10.nih.gov> Oops! I meant answer key instead of study guide. Sorry! Message: 4 Date: Fri, 11 Sep 2015 16:54:24 +0000 From: "Coffey, Anna (NIH/NCI) [C]" > To: "histonet at lists.utsouthwestern.edu" > Subject: [Histonet] Studying for ASCP qIHC Message-ID: <5C3E10119A1B824FBE92B08279F74A91025B2ACC at msgb10.nih.gov> Content-Type: text/plain; charset="us-ascii" Hi everyone, I've been studying for my qIHC exam at the end of this month and I bought the Michigan Society's study workbook which has been a very helpful study guide. It's my understanding that there is no accompanying study guide for this...is that right? If that's not true, I'd love to know where I can pick one up! If it is true, is there anyone (or a group) out there with an interest in developing one? I'd love to work on this with some folks if anyone is interested! Best, Anna From Nancy_Schmitt at pa-ucl.com Fri Sep 11 13:12:29 2015 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Fri, 11 Sep 2015 18:12:29 +0000 Subject: [Histonet] PAP stains done by hand Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C360115A45033@PEITHA.wad.pa-ucl.com> Happy Friday- Could you please share how you are handling the potential for cross contamination in non-gyn pap specimens? Are you filtering/changing out solutions between each case? I appreciate your input- Nancy Histology Coordinator Dubuque, IA 52001 Check us out at www.uclaccess.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Timothy.Morken at ucsf.edu Fri Sep 11 14:26:54 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 11 Sep 2015 19:26:54 +0000 Subject: [Histonet] Cryostat vacuum Message-ID: <761E2B5697F795489C8710BCC72141FF60312E36@ex07.net.ucsf.edu> I just bought one from IMEB : http://www.imebinc.com/ Hello Histonet, Does anyone know where I can find a cryostat vacuum? I've used them in the past but can't seem to find a manufacturer. Thanks! Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net> | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From patrick.lewis at seattlechildrens.org Fri Sep 11 15:03:40 2015 From: patrick.lewis at seattlechildrens.org (Lewis, Patrick) Date: Fri, 11 Sep 2015 20:03:40 +0000 Subject: [Histonet] Endogenous Peroxide staining help Message-ID: <3903BE18914F4440834F0E620415FFCA3CBA0CEE@PPWEXD01d.childrens.sea.kids> Hi everyone, I seem to have a lot of endogenous Peroxide background staining in my FFPE IHC. Human tonsil tissues, with some attached muscle. I do a H202 block at 0.3% H202 in TBS pH 8.0 for 30 minutes. Then I wash x3 with TBST 0.05% Tween20, pH 8.0 Then I serum block with 2% NGS in TBST for 30 minutes. Then I wash x3 TBST Then I add my primary antibody in 2% NGS in TBST overnight. Then I wash x3 TBST Then I add my HRP-labeled 2ndary antibody for 30 minutes. Then I wash x3 TBST Then I have add my AEC Substrate. Should I increase the concentration of H202? Should I increase the time in H202? Is there a different step in the IHC protocol that is better for blocking endogenous H202 activity? Is it possible to lose/reverse the blocking for some reason? I am concerned that my HRP labeled secondary may be to blame. Thoughts? Patrick. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From patrick.lewis at seattlechildrens.org Fri Sep 11 15:33:45 2015 From: patrick.lewis at seattlechildrens.org (Lewis, Patrick) Date: Fri, 11 Sep 2015 20:33:45 +0000 Subject: [Histonet] More on H202 issues Message-ID: <3903BE18914F4440834F0E620415FFCA3CBA0D27@PPWEXD01d.childrens.sea.kids> Hi Everyone Thanks for your responses. I am looking at cell surface markers, Sorry I should have said. Based on what I found out below: Methanol is out, even though I agree that Methanol does enhance the effect of H202 blocking. (I suppose I could try it to see how much/if any epitope loss there is in relation to H202 quenching, At least It would help identifying false positives that are actually H202 artifacts.) Also it looks like increasing the concentration of H202 is out. As to when though, It looks like with cell surface markers I should block after the primary, or even after the 2ndary? Can I do that successfully with a HRP labeled 2ndary? thoughts? Patrick. What solutions or reagents should I use to dilute hydrogen peroxide Methanol, PBS, distilled water or saline can be used to dilute hydrogen peroxide. Morphology of blood smears and peroxidase-rich tissues could be damaged by the aqueous hydrogen peroxide solution. Therefore, methanol is a better choice in this case. Some cell surface markers are very sensitive to methanol/hydrogen peroxide quenching, reducing the staining of antigenic site, particularly on frozen sections. So using hydrogen peroxide in PBS is recommended for cell surface or membrane markers. What concentration of hydrogen peroxide is commonly used 3% hydrogen peroxide is commonly used to block endogenous peroxidase activity. However, certain tissues/cells/antigen (i.e. cell surface markers such as CD4) can be destroyed by high concentration of hydrogen peroxide. So a lower concentration (0.3%) should be used. Where should I do hydrogen peroxide blocking during IHC procedure The blocking can be done (1) after rehydration to water and before antigen retrieval, (2) after antigen retrieval and before primary antibody incubation, (3) after primary antibody incubation, or (4) after biotinylated secondary antibody incubation. For certain antigens such as CD4 and CD8, hydrogen peroxide blocking has detrimental effect on the epitopes, thus reduce intensity of antibody staining. Therefore, blocking after primary antibody or secondary antibody incubation is recommended. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tbraud at holyredeemer.com Sat Sep 12 12:47:09 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Sat, 12 Sep 2015 17:47:09 +0000 Subject: [Histonet] Non-Gyn contamination In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F8037D9A@HRHEX02-HOS.holyredeemer.local> Hi Nancy - Here is what our lab does. All preps (slides)are fixed in individual containers before being batch stained. We stain smears separately from FNAs, and smears and FNAs, separately by case. All fixed thin preps are stained together. With smears or FNAs, we insert a clean slide (labeled as the case number and control) and stain it along with the Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 batch. That slide is examined and if found to have any contaminent from the case, then that triggers a filtration of all reagents and stains, with the container's washed. It is then noted on the stain QC. So far, this has worked very well for us, and our CAP inspectors liked the process and documentation. Regards, Terri 2. PAP stains done by hand (Nancy Schmitt) Message: 2 Date: Fri, 11 Sep 2015 18:12:29 +0000 From: Nancy Schmitt Happy Friday- Could you please share how you are handling the potential for cross contamination in non-gyn pap specimens? Are you filtering/changing out solutions between each case? I appreciate your input- Nancy Histology Coordinator Dubuque, IA 52001 Check us out at www.uclaccess.com ******************************* From amosbrooks at gmail.com Sat Sep 12 17:17:58 2015 From: amosbrooks at gmail.com (Amos Brooks) Date: Sat, 12 Sep 2015 18:17:58 -0400 Subject: [Histonet] More on H202 issues Message-ID: Hi, Peroxidase can really be a pain. If you look in the archives though (or ask her really nice) Gayle Callis submitted a recipe for a glucose oxidase for peroxidase quenching that does not include hydrogen peroxide. If you aren't really a fan of making these things up I would bet dimes to doughnuts that the recipe is *really* similar to the product from Biocare Medical called PeroxAbolish. Here's a link... http://biocare.net/product/peroxabolish/ I have used it and it did work, but I don't really use it regularly so can't really compare it well. Incidentally if I am wrong in my assumptions about the similarity of this to the glucose oxidase, I trust someone here (even from the company itself) will gently correct me. Cheers, Amos On Sat, Sep 12, 2015 at 1:00 PM, wrote: > Message: 5 > Date: Fri, 11 Sep 2015 20:33:45 +0000 > From: "Lewis, Patrick" > To: " (Histonet at lists.utsouthwestern.edu)" > > Subject: [Histonet] More on H202 issues > Message-ID: > > <3903BE18914F4440834F0E620415FFCA3CBA0D27 at PPWEXD01d.childrens.sea.kids> > > Content-Type: text/plain; charset="us-ascii" > > > Hi Everyone > > Thanks for your responses. > > I am looking at cell surface markers, > Sorry I should have said. > > Based on what I found out below: > > Methanol is out, even though I agree that Methanol does enhance the effect > of H202 blocking. > (I suppose I could try it to see how much/if any epitope loss there is in > relation to H202 quenching, At least It would help identifying false > positives that are actually H202 artifacts.) > > Also it looks like increasing the concentration of H202 is out. > > As to when though, > > It looks like with cell surface markers I should block after the primary, > or even after the 2ndary? > Can I do that successfully with a HRP labeled 2ndary? > > thoughts? > > Patrick. > From gayle.callis at bresnan.net Sun Sep 13 12:39:38 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Sun, 13 Sep 2015 11:39:38 -0600 Subject: [Histonet] Long reply on problems with hydrogen peroxide blocking Message-ID: <000301d0ee4b$2a5bc6c0$7f135440$@bresnan.net> Hey Amos, I will be nice! It is true peroxidase blocking can be a pain. However, after looking at the PeroxAbolish SDS from Biocare, I did not SEE any of the chemicals involved in this block so I am skeptical this is the Glucose oxidase method. Big Ho Hum! PeroxAbolish could be similar to a KPL peroxidase block from years past but not sure if KPL still sells it. KPL block was not an enzyme mixture and had to be used carefully on frozen sections. Also, Biocare said nothing about blocking pseudoperoxidases but neither did Vector in their method although the latter did provide the reference which I encourage people to read. The glucose oxidase method was developed for delicate, minimally (acetone) fixed frozen sections and really does work without H2O2 chewing a delicate FS off the slide or damaging morphology. The bonus: glucose oxidase method works on FFPE tissues particularly when an in house H2O2 method fails to remove persistent background caused by peroxidase or pseudoperoxidses. It could be that Patrick is actually getting rid of peroxidase but NOT pseudoperoxidases with his H2O2 method? Glucose oxidase is a good old fashion enzyme/substrate chemical reaction: glucose oxidase + ? D (+) glucose = production of slow steady rate of hydrogen peroxide to remove peroxidase/pseudoperoxidases in tissues and cells. It gave us the cleanest background ever for murine CDmarker/HRP- IHC/solvent fixed frozen sections using a DAB enhancer. We never could get rid of endogenous peroxidase background with a commercial low concentration H2O2 peroxidase block. This is another long story. There is no mystery about the protocol and Vector has this on their website (see below) CAVEAT: the ? D (+) Glucose, 97% pure can be hard to find but here is the source. MP Biomedicals , beta-D-Glucose Catalog Number: 100953. Sigma discontinued this glucose some years ago (heavy sigh!). I will be happy to provide the original publication for this method under separate email. Glucose Oxidase Peroxidase Block (GLUOX), *Jasani et al: Working Solution ? D (+) Glucose, 97% pure (MP Biomedicals Cat. No. 100953) 0.180 g (180 mg) Glucose oxidase (Sigma G 6641) 0.005 g (5 mg) Sodium azide 0.0065g (6.5 mg) Dulbeccos PBS 50 ml Do not preheat working solution. Protocol: 1. Immerse sections in working glucose oxidase solution 30 min - 1 hr (per cited reference). Use a 37?C water bath for even heating. 2. After incubation, rinse sections 3X in DPBS, 5 min per change then proceed to IHC. Results: Clean background without endogenous peroxidase or pseudoperoxidases in minimally fixed (acetone) FS or FFPE. NOTES: A. Glucose oxidase can be weighed into screw top micro-centrifuge tube and stored in a freezer. Pay attention to the expiration date and storage conditions. This way, one can make up a ready to use stock buffer in large quantity i.e. 2 liters or more. We called this "GLUOX Buffer" due to sodium azide content. Weighing out chemicals each time was painful to say the least. A stock buffer permitted quick preparation for a desired working solution volume e.g. 50 ml or more. To prepare desired quantity of working soluton: pipette 500 ?l GLUOX buffer into micro-centrifuge tube containing pre-weighed glucose oxidase, vortex mix to dissolve, add mixture remaining volume of buffer, stir, pour into staining container/coplin jar. B. Use a 37?C water bath for even heating, NOT AN INCUBATOR with air currents causing uneven heating. Reference: *Andrew SM, Jasani B. An improved method for the inhibition of endogenous peroxidase non-deleterious to lymphocyte surface markers. Application to immunoperoxidase studies on eosinophil-rich tissue preparations. Histochem J 19:426-430, 1987 FYI: Vector has this method in a free downloadable pdf brochure; http://vectorlabs.com/brochures/ Multiple Antigen Labeling Guide p.20/Appendix 2/Method 3 I took the liberty of copying this from Vector who used mg terminology. Method 3. 0.180 g b-D(+) glucose, 5 mg glucose oxidase, 6.5 mg sodium azide in 50 ml PBS. Incubate sections for 1 hour at 37 ?C. Rinse in PBS 3 x 5 minutes. This reaction slowly and steadily produces very low concentrations of H2O2 by enzymatic reaction. This method consistently and completely inhibits peroxidase activity. (Andrew S.M., Jasani, B.; Histochem J. 19, 426-430,1987.) Final comment. If Patrick still has background staining after using Glucose oxidase method, then I suspect background is coming from another source which can be determined with an immunostaining reagent background test. I will be happy to provide the simple background test too. Take care Gayle Callis HTL/HT/MT(ASCP) Amos and Patrick Wrote: Hi, Peroxidase can really be a pain. If you look in the archives though (or ask her really nice) Gayle Callis submitted a recipe for a glucose oxidase for peroxidase quenching that does not include hydrogen peroxide. If you aren't really a fan of making these things up I would bet dimes to doughnuts that the recipe is *really* similar to the product from Biocare Medical called PeroxAbolish. Here's a link... http://biocare.net/product/peroxabolish/ I have used it and it did work, but I don't really use it regularly so can't really compare it well. Incidentally if I am wrong in my assumptions about the similarity of this to the glucose oxidase, I trust someone here (even from the company itself) will gently correct me. Cheers, Amos On Sat, Sep 12, 2015 at 1:00 PM, > wrote: > Message: 5 > Date: Fri, 11 Sep 2015 20:33:45 +0000 > From: "Lewis, Patrick" > > > Hi Everyone > > Thanks for your responses. > > I am looking at cell surface markers, > Sorry I should have said. > > Based on what I found out below: > > Methanol is out, even though I agree that Methanol does enhance the effect > of H202 blocking. > (I suppose I could try it to see how much/if any epitope loss there is in > relation to H202 quenching, At least It would help identifying false > positives that are actually H202 artifacts.) > > Also it looks like increasing the concentration of H202 is out. > > As to when though, > > It looks like with cell surface markers I should block after the primary, > or even after the 2ndary? > Can I do that successfully with a HRP labeled 2ndary? > > thoughts? > > Patrick. From TanyaAbbott at catholichealth.net Mon Sep 14 07:03:46 2015 From: TanyaAbbott at catholichealth.net (Abbott, Tanya) Date: Mon, 14 Sep 2015 12:03:46 +0000 Subject: [Histonet] Grossing Histotechs Message-ID: <852F7D2C14FB464D80E182B15DB138AF70271410@CHIEX005.CHI.catholichealth.net> So, are there a lot of Histotechs out there who are still grossing? Or is this job being done by PAs? Thanks! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology Penn State Health St. Joseph (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From kdonadio at ymail.com Mon Sep 14 08:40:40 2015 From: kdonadio at ymail.com (Kim Donadio) Date: Mon, 14 Sep 2015 13:40:40 +0000 (UTC) Subject: [Histonet] Grossing Histotechs In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF70271410@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF70271410@CHIEX005.CHI.catholichealth.net> Message-ID: <88518372.2281773.1442238041196.JavaMail.yahoo@mail.yahoo.com> I've seen mostly histotechs who meet CLIA high complexity guidelines doing gross dissection. In my limited 20 years/5 hospitals I've only seen certified PAs at one place,?Mayo.?Kim D ? From: "Abbott, Tanya via Histonet" To: "histonet at lists.utsouthwestern.edu" Sent: Monday, September 14, 2015 7:03 AM Subject: [Histonet] Grossing Histotechs So, are there a lot of Histotechs out there who are still grossing? Or is this job being done by PAs? Thanks! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology Penn State Health St. Joseph (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From blayjorge at gmail.com Mon Sep 14 09:12:23 2015 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Mon, 14 Sep 2015 10:12:23 -0400 Subject: [Histonet] Looking for Human A and P Listservers Message-ID: Dear Histonetetrs: I have been looking for Human A and P Listservers and, thus far, I have not been successful. Will appreciate recommendations of such listservers send directly to my email: blayjorge at gmail.com With gratefulness, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From PKRichar at gundersenhealth.org Mon Sep 14 11:43:13 2015 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Mon, 14 Sep 2015 16:43:13 +0000 Subject: [Histonet] Histology Roles Message-ID: <998284C32F61104CA0BEFFFFCF6F90FD6CFABB44@LXEXMB01.gundluth.org> Hi, I am wondering how many roles within histology your lab has? We currently have two PAs, eight Histology Technicians, and five Lab Prep Techs. Anyone want to share how many roles you have and how the work is divided? Thanks in advance. Cordially, Pam ~ +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org From j.benavides at eae.csic.es Mon Sep 14 11:52:02 2015 From: j.benavides at eae.csic.es (Julio Benavides) Date: Mon, 14 Sep 2015 18:52:02 +0200 Subject: [Histonet] Eosinophil labelling In-Reply-To: <3903BE18914F4440834F0E620415FFCA3CBA0CEE@PPWEXD01d.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA3CBA0CEE@PPWEXD01d.childrens.sea.kids> Message-ID: <55F6FB32.4030903@eae.csic.es> Hi everyone, Is there anti antibody , working on FFPE, labelling eosinophils? if it works in sheep then that?s perfect! I have seen several histochemical methods (Modified Congo Red, Luna Protocol, Astra Blue/Vital New Red Protocol (AB/VNR, Sirius Red Stai ). Does anyone has any experience with them? Any help/comments in this issues would be, as always, greatly appreciated!!! Thank you Thanks a lot! Julio From gu.lang at gmx.at Mon Sep 14 12:28:27 2015 From: gu.lang at gmx.at (Gudrun Lang) Date: Mon, 14 Sep 2015 19:28:27 +0200 Subject: [Histonet] Eosinophil labelling In-Reply-To: <55F6FB32.4030903@eae.csic.es> References: <3903BE18914F4440834F0E620415FFCA3CBA0CEE@PPWEXD01d.childrens.sea.kids> <55F6FB32.4030903@eae.csic.es> Message-ID: <000f01d0ef12$c6c2cf50$54486df0$@gmx.at> What about simple H&E? Gudrun -----Urspr?ngliche Nachricht----- Von: Julio Benavides via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Montag, 14. September 2015 18:52 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Eosinophil labelling Hi everyone, Is there anti antibody , working on FFPE, labelling eosinophils? if it works in sheep then that?s perfect! I have seen several histochemical methods (Modified Congo Red, Luna Protocol, Astra Blue/Vital New Red Protocol (AB/VNR, Sirius Red Stai ). Does anyone has any experience with them? Any help/comments in this issues would be, as always, greatly appreciated!!! Thank you Thanks a lot! Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks at gmail.com Mon Sep 14 15:00:06 2015 From: amosbrooks at gmail.com (Amos Brooks) Date: Mon, 14 Sep 2015 16:00:06 -0400 Subject: [Histonet] H2O2 blocking Message-ID: Hi Gayle, Thank you again for the insight. You are a wealth of knowledge. I am also not particularly surprised that I was incorrect in my assumption about Peroxabolish. I really like to know what is in the products I am using. That's why I prefer to make up my own reagents whenever possible, and why I really like your method. Amos From mdmurphy at alaska.edu Mon Sep 14 16:50:09 2015 From: mdmurphy at alaska.edu (Molly Murphy) Date: Mon, 14 Sep 2015 13:50:09 -0800 Subject: [Histonet] stains for corpus luteum Message-ID: Hello all, What is your favorite histo stain for corpus luteum? Looking for something to really make them stand out. Thanks! -- Molly Murphy DVM, Ph.D Assistant Professor of Veterinary Pathology College of Natural Sciences & Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 From akemiat3377 at gmail.com Tue Sep 15 08:35:02 2015 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Tue, 15 Sep 2015 06:35:02 -0700 Subject: [Histonet] Hiring a histologist Message-ID: <54A3BD47-845E-4C4D-9F55-B77C7577CE3F@gmail.com> Attention recently graduated certified or certified eligible histologists! Our GI practice in Beautiful Monterey, CA is expanding, and we are going to hire another histologist to hone their skills under my tutelage. Must meet CLIA grossing regulations. We offer full benefits and 401 K. If you are interested, or know of someone who would be interested, contact me and I will give you more details. akemiat3377 at gmail.com From Richard.Cartun at hhchealth.org Tue Sep 15 09:18:45 2015 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 15 Sep 2015 14:18:45 +0000 Subject: [Histonet] Patient identifiers Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B042B55@HHCEXCHMB03.hhcsystem.org> What are people accepting for the "two" identifiers on the small formalin bottles that are used for prostate biopsy specimens? Can you accept the patient's initials in place of the full name? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From wbenton at cua.md Tue Sep 15 09:42:44 2015 From: wbenton at cua.md (Walter Benton) Date: Tue, 15 Sep 2015 14:42:44 +0000 Subject: [Histonet] Patient identifiers In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E6B042B55@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E6B042B55@HHCEXCHMB03.hhcsystem.org> Message-ID: <481ebc2a40e04943a5526a9ea3f9a0d0@MAIL01.GCU-MD.local> Full name and date of birth. -----Original Message----- From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 15, 2015 10:19 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Patient identifiers What are people accepting for the "two" identifiers on the small formalin bottles that are used for prostate biopsy specimens? Can you accept the patient's initials in place of the full name? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From Richard.Cartun at hhchealth.org Tue Sep 15 12:10:00 2015 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 15 Sep 2015 17:10:00 +0000 Subject: [Histonet] Patient identifiers Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B042C8E@HHCEXCHMB03.hhcsystem.org> Thanks to all who responded to my question on patient identifiers for prostate biopsies. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From KSimeone at leavittmgt.com Wed Sep 16 09:09:01 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Wed, 16 Sep 2015 14:09:01 +0000 Subject: [Histonet] FULLTIME DAY SHIFT (first shift) POSITION DELRAY BCH FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C4D2DD71D@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Beach, Florida dermatology laboratory. This is a permanent full time, DAYTIME SHIFT (40 hours) position with benefits (medical/401k/vacation). Annual salary in the $50k range. THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! PLEASE VISIT THIS LINK TO APPLY: https://advancedderm.applicantpro.com/jobs/240090.html *full time position Mon-Fri 8a-5:30p (start time may vary) *MUST be licensed as a FLORIDA HISTOTECHNICIAN OR HISTOTECHNOLOGIST (NO pending licensure pls) *PREFER experience but WILL TRAIN a knowledgeable, willing candidate *duties to include (but not limited to): grossing, microtomy, accessioning, embedding and general histology *must be self motivated, reliable and a team player Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From HornHV at archildrens.org Thu Sep 17 08:49:14 2015 From: HornHV at archildrens.org (Horn, Hazel V) Date: Thu, 17 Sep 2015 08:49:14 -0500 Subject: [Histonet] dictation systems Message-ID: <25A4DE08332B19499904459F00AAACB71A39C4FC8F@EVS1.archildrens.org> All, We are looking for a new dictation system for grossing. Will you please share what you use? We still have a Lanier microcassette system and it's outdated and not made anymore. Thanks. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org From kim-lake at uiowa.edu Thu Sep 17 09:01:08 2015 From: kim-lake at uiowa.edu (Lake, Kim S) Date: Thu, 17 Sep 2015 14:01:08 +0000 Subject: [Histonet] Looking for a Olympus BH2 microscope bag -- please sell me one of yours! Message-ID: One of our residents is going to take her boards in a few months, and she would like to take along her (ancient!) Olympus BH2 microscope. Does anyone have a BH2 microscope travel bag/case that they would be willing to sell to us? Alternatively, does anyone have any favorite retailers of vintage microscope bags that they can recommend? Thanks! Kim Lake MT(ASCP) Laboratory Manager University of Iowa Oral Pathology Laboratory S387 Dental Science Building Iowa City, IA 52242 Phone: 319 384 4433 Fax: 319 353 5569 Email: kim-lake at uiowa.edu From dlschneider at gmail.com Thu Sep 17 09:32:54 2015 From: dlschneider at gmail.com (Daniel Schneider) Date: Thu, 17 Sep 2015 09:32:54 -0500 Subject: [Histonet] Looking for a Olympus BH2 microscope bag -- please sell me one of yours! In-Reply-To: References: Message-ID: She shouldn't sweat it. When I took the boards I don't recall anyone bringing their own scope. I'm sure people do it, but it's overkill. Someone should gently encourage her to use the microscope provided on-site. It works the same as her BH2. I can't remember what make it was but the board can tell her. I had no trouble with it -- there were no surprises with respect to the scope. And then she won't have to worry about lugging her scope across the country. On Thu, Sep 17, 2015 at 9:01 AM, Lake, Kim S via Histonet < histonet at lists.utsouthwestern.edu> wrote: > One of our residents is going to take her boards in a few months, and she > would like to take along her (ancient!) Olympus BH2 microscope. Does anyone > have a BH2 microscope travel bag/case that they would be willing to sell to > us? > > Alternatively, does anyone have any favorite retailers of vintage > microscope bags that they can recommend? > > Thanks! > > > Kim Lake MT(ASCP) > Laboratory Manager > University of Iowa Oral Pathology Laboratory > S387 Dental Science Building > Iowa City, IA 52242 > > Phone: 319 384 4433 > Fax: 319 353 5569 > Email: kim-lake at uiowa.edu > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 at earthlink.net Thu Sep 17 10:22:21 2015 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 17 Sep 2015 11:22:21 -0400 Subject: [Histonet] Relia Hot Histology Job Alert - 9-17-2015 A quick note with some new opportunities to pass along. Message-ID: <001a01d0f15c$a5d2b840$f17828c0$@earthlink.net> Hi Histopeeps! How are you today? I hope your week is going well! I know mine is. I wanted to drop you a quick line about a couple of the jobs I am working on. If you or anyone you know is interested in more details I can be reached toll free at 866-607-3542 or relia1 at earthlink.net. If you are starting to save up your Christmas money remember I pay a referral fee to you if I place someone that you refer to me. Here are the current opportunities: Management: IHC Supervisor - Long Island, NY AP Manager - Hammond, IN Histology Supervisor - Flagstaff, AZ Histotechnician/Histotechnologist: Austin, TX Lafayette, LA Atlanta, GA IHC Tech - Norfolk, VA (15K sign on bonus!) East of Tyler, TX (Dermpath learn Mohs!) Montgomery, AL Remember all of these positions are permanent and full time and have excellent benefits. Also in most cases the clients are assisting with relocation. And some are offering sign on bonuses!! All of them are eager to interview and hire today!! Thanks and have a great day!! Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Toll Free:(866)607-3542 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia From b-frederick at northwestern.edu Thu Sep 17 10:29:04 2015 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Thu, 17 Sep 2015 15:29:04 +0000 Subject: [Histonet] Oil Red O Message-ID: <3aff67d194ff421a8f5628efe3f4bb2b@evcspmbx03.ads.northwestern.edu> Hello all, Will Oil Red O stain phospholipids? We will be helping another lab with some work and the project out line states that Phopslipids are externalized on the cell surface during apoptosis. Can always try it I suppose. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu From jill.cox at cox.net Thu Sep 17 12:30:51 2015 From: jill.cox at cox.net (Jill Cox) Date: Thu, 17 Sep 2015 10:30:51 -0700 Subject: [Histonet] UPS for VIP 5 Message-ID: Hello Netters! Looking to purchase a UPS for our VIP 5 and was hoping you could help! I received a quote and got sticker shock so hoping there are other options out there or at least less expensive. Thank you!! Jill Cox Homesmart Real Estate 602-481-1424 From wdesalvo.cac at outlook.com Thu Sep 17 12:32:36 2015 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Thu, 17 Sep 2015 12:32:36 -0500 Subject: [Histonet] dictation systems Message-ID: At our lab, we have used a digital server system called Fusion. I suggest you go digital and then consider if you can also move to voice recognition and activation for efficiency. There are so many more options now as compared to the tape systems. Sent from my Windows Phone ________________________________ From: Horn, Hazel V via Histonet Sent: ?9/?17/?2015 9:00 AM To: histonet (histonet at lists.utsouthwestern.edu) Subject: [Histonet] dictation systems All, We are looking for a new dictation system for grossing. Will you please share what you use? We still have a Lanier microcassette system and it's outdated and not made anymore. Thanks. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kcastillo at swskin.net Thu Sep 17 13:49:44 2015 From: kcastillo at swskin.net (Kristy Castillo) Date: Thu, 17 Sep 2015 11:49:44 -0700 Subject: [Histonet] Slide printer Message-ID: I have an old Tissue Tek Auto Write slide printer. Would like to know if anyone is interested in it for the parts. We are relocating our Pathology lab and really do not want to take it with us. Thank you! Kristy Castillo Pathology Lab Supervisor ________________________________ This transmission may contain confidential information, some or all of which may be protected health information as defined by the federal Health Insurance Portability & Accountability Act (HIPAA) Privacy Rule. This transmission is intended for the exclusive use of the individual or entity to whom it is addressed and may contain information that is proprietary, privileged, confidential and/or exempt from disclosure under applicable law. If you are not the intended recipient (or an employee or agent responsible for delivering this transmission to the intended recipient), you are hereby notified that any disclosure, dissemination, distribution or copying of this information is strictly prohibited and may be subject to legal restriction or sanction. Please notify the sender by telephone to arrange the return or destruction of the information and all copies. From Joyce.Weems at emoryhealthcare.org Thu Sep 17 14:08:20 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Thu, 17 Sep 2015 19:08:20 +0000 Subject: [Histonet] Case distribution Message-ID: Hello Folks, Would you all please share your fair and just distribution of cases so that your pathologists are doing an equal share of work? I'd appreciate it! Thanks, j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From litepath2000 at yahoo.com Thu Sep 17 15:04:14 2015 From: litepath2000 at yahoo.com (NYSHisto) Date: Thu, 17 Sep 2015 20:04:14 +0000 (UTC) Subject: [Histonet] veterinary IHC Message-ID: <1964723234.1183702.1442520254317.JavaMail.yahoo@mail.yahoo.com> Looking for academic facility/lab that runs IHC on canines samples.? Please contact me of list.Thanks in advance From b-frederick at northwestern.edu Fri Sep 18 08:21:40 2015 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Fri, 18 Sep 2015 13:21:40 +0000 Subject: [Histonet] CD34 Message-ID: <536309ecab7c4539b03f1e9cc1c54a9a@evcspmbx03.ads.northwestern.edu> Been looking around a bit and seem to have it a roadblock. Does anyone know of a vendor for CD34 that will cross react with rabbit? On frozen sections, mind you. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu From Nancy_Schmitt at pa-ucl.com Fri Sep 18 10:09:19 2015 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Fri, 18 Sep 2015 15:09:19 +0000 Subject: [Histonet] H. Pylori Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C360115A48744@PEITHA.wad.pa-ucl.com> Good Morning- I am looking for H. Pylori control - does anyone have any extra lying about? What are you currently using? Purchasing? I appreciate any and all feedback! Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA 52001 Check us out at www.uclaccess.com NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From PKRichar at gundersenhealth.org Fri Sep 18 10:40:54 2015 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Fri, 18 Sep 2015 15:40:54 +0000 Subject: [Histonet] IHC specialist Message-ID: <998284C32F61104CA0BEFFFFCF6F90FD6CFB0C34@LXEXMB01.gundluth.org> Would anyone be able to provide a job description for an IHC specialists? Cordially, Pam ~ +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org From kaarrington at anthc.org Fri Sep 18 12:24:15 2015 From: kaarrington at anthc.org (Arrington, Karla A) Date: Fri, 18 Sep 2015 17:24:15 +0000 Subject: [Histonet] Source Codes Message-ID: To All: Our hospital is going over their specimen source codes and updating them. I am asking for suggestions in finding out if there is an already list out there that includes all the specimen sources and CPT codes. Also, if one gets a multiple organs specimen received in one container, can one code the organs individually, or is just simply one code because it came in one container? Thanks, Karla Arrington, HT(ASCP) HIT(AHIMA) Pathology Supervisor ANMC 4315 Diplomacy Drive Anchorage, AK 99508 Phone: 907-729-1810 Fax: 907-729-1226 kaarrington at anthc.org From Elise_ODea at URMC.Rochester.edu Fri Sep 18 12:28:45 2015 From: Elise_ODea at URMC.Rochester.edu (ODea, Elise) Date: Fri, 18 Sep 2015 17:28:45 +0000 Subject: [Histonet] Tissue Processors Reviews Message-ID: <38faebae6f914e93be13e25a379172d0@exmbxpdc06.urmc-sh.rochester.edu> I am looking for experiences and opinions on Sakura VIP6 and Excelsior processors? Thank you, Elise Elise T. O'Dea, MT, ASCP Histology Supervisor Highland Hospital 1000 South Ave. Rochester, New York 14620 office 585.341.6596 lab 585.341.8314 elise_odea at urmc.rochester.edu Confidentiality Notice This transmission contains confidential information protected by New York State law and HIPAA regulations. You are prohibited from making any further disclosure of this information without the specific written consent of the person to whom it pertains, or as otherwise permitted by law. A general authorization for the release of medical or other information is not sufficient authorization for further disclosure of information, which is protected by New York State Public Health Law Article 27-F or Title 42 of the Code of Federal Regulations. Any unauthorized further disclosure in violation of State law may result in a fine or jail sentence or both. If you have received this material in error, please notify the sender IMMEDIATELY to arrange for the return or destruction of the document(s). From JRobinson at pathology-associates.com Fri Sep 18 13:01:00 2015 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Fri, 18 Sep 2015 18:01:00 +0000 Subject: [Histonet] Retics on Ventana Benchmark Special Stainer Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16DA00E5@PAEXCH1.PathologyAssociates.local> Good Morning to all Histotechs- There have been many questions over the past several months regarding poor retic staining on the Ventana Benchmark Special Stainer. With the help of my histotech friends, I have now achieved successful staining on both liver and bone marrow specimens utilizing this instrument. My primary source of protocol information has been Heather Nowacki at the Mayo Clinic. Heather says that they use B-5 fixative and RDO Gold decalcifier on their bone marrow cores. We use B Plus fixative and Immunocal decalcifier so the choice of an appropriate fixative and decalcifier does not seem to be a concern in the ability of this instrument to achieve the desired retic staining intensity. Here is Heather's protocol for retic: Heat: 45 degrees C. Oxidizer: 4 minutes Decolorizer: 4 minutes Sensitizer: 8 minutes Silver: 12 minutes Nuclear Fast Red Counterstain: 4 minutes I did not need to modify any of Heather's protocol options. I had previously been unable to achieve any satisfactory staining on bone marrow retic staining on the Benchmark Special Stainer. I showed my bone marrow retic test slides to one of my pathologists and he rated them comparable to the retic slides we are currently staining on the old Ventana Nexes stainer. Notes: It seems that the primary issue affecting proper retic staining on the Benchmark Special Stainer has been the lack of sufficient heat applied to the slides during the staining run. Ventana has released a software upgrade that allows for a much wider range of heating temperature options during retic staining. You will need to have the new software upgrade installed by Ventana before you will be able to use this protocol. Ventana also discussed an enhanced decontamination protocol for the Benchmark Special Stainer that requires some additional reagents but the Ventana technical rep who installed my upgrade determined that the enhanced decontamination protocol was not necessary in our lab. She felt that this enhanced protocol would only be beneficial in labs that have had major contamination issues (including Special Stains Wash pH changes) in the past. We cut all of our retic slides at 5 (even for the Nexes) and I have been quite happy with the staining intensity on slides cut at that thickness. Slides cut at 3 or 4 stain too light for my taste. Acknowledgements: I would like to thank Tim Morken for spotting the Mayo Clinic poster on Ventana Benchmark Special Stainer Retic staining at NSH last month. I would also like to thank the team at the Mayo Clinic who worked on this project allowing the rest of us in the field to benefit from their hard work. The Mayo team includes: Heather Nowacki, HTL; Mark Keith, HTL; Scott Antilla, HTL; and Joaquin Garcia, M.D. I hope this information is of use to my fellow histotechs who have the Ventana Benchmark Special Stainer in their labs. Jeff Robinson, HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From Richard.Cartun at hhchealth.org Fri Sep 18 13:20:16 2015 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 18 Sep 2015 18:20:16 +0000 Subject: [Histonet] H. pylori control tissue Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B04B9B6@HHCEXCHMB03.hhcsystem.org> Nancy Schmitt from Dubuque, IA asked about control tissue for H. pylori. I have tried e-mailing her, but my e-mail was returned as "Undeliverable". Nancy, please contact me. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From josephnerk at hotmail.com Fri Sep 18 17:37:31 2015 From: josephnerk at hotmail.com (Erskine Husbands) Date: Fri, 18 Sep 2015 17:37:31 -0500 Subject: [Histonet] Validation Message-ID: Does anyone in Histoland have any information on validation of H.pylori immunohistochemical staining, From rachel at gbi-inc.com Fri Sep 18 18:19:55 2015 From: rachel at gbi-inc.com (Rachel M Gonzalez) Date: Fri, 18 Sep 2015 19:19:55 -0400 Subject: [Histonet] Tissue request.... Message-ID: Hi Looking for control tissue for reviewing antibody specificity this is for R&D project. I do not know if that will make a difference in the tissue availability but I thought I put that out there. If you know a good tissue source can you let me know. I need three of each type normal bone marrow (hematopoietic cell and lymphocytes, and etc.), normal brain/cerebrum, normal cerebellum, normal parathyroid glands, normal hypophysis/Pituitary Gland normal thymus. Thank you in advance for help. Rachel Gonzalez PhD Senior Scientist . From sadrew at wisc.edu Fri Sep 18 18:47:18 2015 From: sadrew at wisc.edu (SALLY A DREW) Date: Fri, 18 Sep 2015 23:47:18 +0000 Subject: [Histonet] Tissue request.... In-Reply-To: References: Message-ID: Just a suggestion...Is this need for materials for review for formalin-fixed, paraffin-embedded tissues? Is this for human tissue? These are just two questions that need to be answered in order before any willing source can speak up. Sent from my U.S. Cellular? Smartphone -------- Original message -------- From: Rachel M Gonzalez via Histonet Date: 09/18/2015 6:20 PM (GMT-06:00) To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Tissue request.... Hi Looking for control tissue for reviewing antibody specificity this is for R&D project. I do not know if that will make a difference in the tissue availability but I thought I put that out there. If you know a good tissue source can you let me know. I need three of each type normal bone marrow (hematopoietic cell and lymphocytes, and etc.), normal brain/cerebrum, normal cerebellum, normal parathyroid glands, normal hypophysis/Pituitary Gland normal thymus. Thank you in advance for help. Rachel Gonzalez PhD Senior Scientist . _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rachel at gbi-inc.com Sat Sep 19 10:31:39 2015 From: rachel at gbi-inc.com (Rachel M Gonzalez) Date: Sat, 19 Sep 2015 12:31:39 -0300 Subject: [Histonet] Tissue request.... In-Reply-To: References: Message-ID: Hi I need human tissue and FFPE blocks. Thanks Rachel On Fri, Sep 18, 2015 at 8:47 PM, SALLY A DREW wrote: > Just a suggestion...Is this need for materials for review for > formalin-fixed, paraffin-embedded tissues? Is this for human tissue? These > are just two questions that need to be answered in order before any willing > source can speak up. > > > > Sent from my U.S. Cellular? Smartphone > > > -------- Original message -------- > From: Rachel M Gonzalez via Histonet > Date: 09/18/2015 6:20 PM (GMT-06:00) > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Tissue request.... > > Hi > > Looking for control tissue for reviewing antibody specificity this is for > R&D project. I do not know if that will make a difference in the tissue > availability but I thought I put that out there. If you know a good tissue > source can you let me know. > > I need three of each type > > normal bone marrow (hematopoietic cell and lymphocytes, and etc.), > > normal brain/cerebrum, > > normal cerebellum, > > normal parathyroid glands, > > normal hypophysis/Pituitary Gland > > normal thymus. > > > > Thank you in advance for help. > > Rachel Gonzalez PhD > > Senior Scientist > . > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From brett_connolly at merck.com Sat Sep 19 14:31:03 2015 From: brett_connolly at merck.com (Connolly, Brett M) Date: Sat, 19 Sep 2015 15:31:03 -0400 Subject: [Histonet] Tissue request.... Message-ID: Rachel, If you have funding to purchase you can check out these biorepositories... Asterand Bioscience http://www.asterandbio.com/ National Disease Research Interchange http://ndriresource.org/ Cooperative Human Tissue Network http://www.chtn.nci.nih.gov/ Analytical Biological Services http://www.absbio.com/ Cureline Laboratories http://www.cureline.com/ Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Translational Biomarkers Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 ________________________________________ From: Rachel M Gonzalez via Histonet [histonet at lists.utsouthwestern.edu] Sent: Friday, September 18, 2015 7:19 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Tissue request.... Hi Looking for control tissue for reviewing antibody specificity this is for R&D project. I do not know if that will make a difference in the tissue availability but I thought I put that out there. If you know a good tissue source can you let me know. I need three of each type normal bone marrow (hematopoietic cell and lymphocytes, and etc.), normal brain/cerebrum, normal cerebellum, normal parathyroid glands, normal hypophysis/Pituitary Gland normal thymus. Thank you in advance for help. Rachel Gonzalez PhD Senior Scientist . _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From rob at foliobio.com Sat Sep 19 15:25:31 2015 From: rob at foliobio.com (Rob Day) Date: Sat, 19 Sep 2015 16:25:31 -0400 Subject: [Histonet] Tissue request.... In-Reply-To: References: Message-ID: <17E3A98D-1017-4BE6-9B4C-57C1658B57D7@foliobio.com> Or this one: http://lab-ally.com/products/ffpe-biospecimens/ or any of the many listed here: http://specimencentral.com/biobank-directory/ > On Sep 19, 2015, at 3:31 PM, Connolly, Brett M via Histonet wrote: > > Rachel, > > If you have funding to purchase you can check out these biorepositories... > > Asterand Bioscience > http://www.asterandbio.com/ > > National Disease Research Interchange > http://ndriresource.org/ > > Cooperative Human Tissue Network > http://www.chtn.nci.nih.gov/ > > Analytical Biological Services > http://www.absbio.com/ > > Cureline Laboratories > http://www.cureline.com/ > > > Brett M. Connolly, Ph.D. > Principle Scientist, Imaging Dept. > Translational Biomarkers > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly at merck.com > T- 215-652-2501 > F- 215-993-6803 > > > > > ________________________________________ > From: Rachel M Gonzalez via Histonet [histonet at lists.utsouthwestern.edu] > Sent: Friday, September 18, 2015 7:19 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Tissue request.... > > Hi > > Looking for control tissue for reviewing antibody specificity this is for > R&D project. I do not know if that will make a difference in the tissue > availability but I thought I put that out there. If you know a good tissue > source can you let me know. > > I need three of each type > > normal bone marrow (hematopoietic cell and lymphocytes, and etc.), > > normal brain/cerebrum, > > normal cerebellum, > > normal parathyroid glands, > > normal hypophysis/Pituitary Gland > > normal thymus. > > > > Thank you in advance for help. > > Rachel Gonzalez PhD > > Senior Scientist > . > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, > New Jersey, USA 07033), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Rob Day Business Development Folio Biosciences 1476 Manning Pkwy, Powell, Ohio 43065 Direct Line: 614-407-4547 Fax: 877-633-6442 skype: invasifspecies http://foliobio.com www.linkedin.com/in/robdaybiotech This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. Visit my blogs here. From adhall at ael.com Mon Sep 21 06:46:26 2015 From: adhall at ael.com (Angela Hall) Date: Mon, 21 Sep 2015 06:46:26 -0500 Subject: [Histonet] Tissue Processors Reviews In-Reply-To: <38faebae6f914e93be13e25a379172d0@exmbxpdc06.urmc-sh.rochester.edu> References: <38faebae6f914e93be13e25a379172d0@exmbxpdc06.urmc-sh.rochester.edu> Message-ID: Elise, We have used a Sakura VIP 6 since 2010 and seem to have very little trouble with it. Specimens process very well. It's very user friendly, in my opinion. As far as problems, the yearly PM was last week and necessitated in one day of downtime due to a problem with the pump. Our PM tech had the part overnighted and the processor was fixed the next day. OH, funny story, the first year that we had it, someone sprayed the screen with Windex causing an issue with the screen. Angela D. Hall, BA, HT(ASCP)CM Lead Histotechnician American Esoteric Laboratories www.ael-east.com ________________________________ Tel +423 586 3240 ext 1019 or 1041 Fax +423 714 2001 ? This message and any files transmitted with it may contain privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message, you must not disseminate, copy or take any action in reliance on it. If you have received this message in error, please notify?the?sender ?immediately. -----Original Message----- From: ODea, Elise [mailto:Elise_ODea at URMC.Rochester.edu] Sent: Friday, September 18, 2015 1:29 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Tissue Processors Reviews I am looking for experiences and opinions on Sakura VIP6 and Excelsior processors? Thank you, Elise Elise T. O'Dea, MT, ASCP Histology Supervisor Highland Hospital 1000 South Ave. Rochester, New York 14620 office 585.341.6596 lab 585.341.8314 elise_odea at urmc.rochester.edu Confidentiality Notice This transmission contains confidential information protected by New York State law and HIPAA regulations. You are prohibited from making any further disclosure of this information without the specific written consent of the person to whom it pertains, or as otherwise permitted by law. A general authorization for the release of medical or other information is not sufficient authorization for further disclosure of information, which is protected by New York State Public Health Law Article 27-F or Title 42 of the Code of Federal Regulations. Any unauthorized further disclosure in violation of State law may result in a fine or jail sentence or both. If you have received this material in error, please notify the sender IMMEDIATELY to arrange for the return or destruction of the document(s). From Elise_ODea at URMC.Rochester.edu Mon Sep 21 07:12:53 2015 From: Elise_ODea at URMC.Rochester.edu (ODea, Elise) Date: Mon, 21 Sep 2015 12:12:53 +0000 Subject: [Histonet] Tissue Processor Reviews Message-ID: Thank you all for your opinions and experiences of Excelsior and Sakura! Elise From PKRichar at gundersenhealth.org Mon Sep 21 09:21:42 2015 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Mon, 21 Sep 2015 14:21:42 +0000 Subject: [Histonet] IHC specialist Message-ID: <998284C32F61104CA0BEFFFFCF6F90FD6CFB294D@LXEXMB01.gundluth.org> I asked last week and didn't get a response so I thought I would try one more time. Is there any IHC specialists out there that could share your job description? Thanks in advance. Cordially, Pam ~ +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org From patpxs at gmail.com Mon Sep 21 10:10:28 2015 From: patpxs at gmail.com (P Sicurello) Date: Mon, 21 Sep 2015 08:10:28 -0700 Subject: [Histonet] Elastic on the Ventana BenchMark Special Stainer Message-ID: Good Morning Netters, Has anyone had any luck running the Elastic program and getting the elastic fibers to be black? All I'm getting is purple no matter how I modify the program. Even one of the application specialists told me that he can't get the fibers to turn black either. If you have a program modification to turn those puppies black, please send me the protocol. Thanks oodles, Paula :-) UCSD Health System From cheastys at svm.vetmed.wisc.edu Mon Sep 21 15:14:39 2015 From: cheastys at svm.vetmed.wisc.edu (Sandra Cheasty) Date: Mon, 21 Sep 2015 15:14:39 -0500 Subject: [Histonet] Hematoxylin Precipitate Message-ID: <4cda87133587e64c965ce6c356d18f59@svm.vetmed.wisc.edu> Hello all, Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water bath or our water supply on the stainer. I used sterile gloves, opened a new case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, air-dried and coverslipped them, and the blue dots were there. The only way we got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive. Thanks for your input, and if you can recommend a different, reasonably priced hematoxylin, that would be awesome. Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From claycal44 at yahoo.com Mon Sep 21 16:17:41 2015 From: claycal44 at yahoo.com (nancy lowen) Date: Mon, 21 Sep 2015 21:17:41 +0000 (UTC) Subject: [Histonet] Hematoxylin Precipitate In-Reply-To: <4cda87133587e64c965ce6c356d18f59@svm.vetmed.wisc.edu> References: <4cda87133587e64c965ce6c356d18f59@svm.vetmed.wisc.edu> Message-ID: <1130699889.1214692.1442870261501.JavaMail.yahoo@mail.yahoo.com> Yes, I have had the same experience.Filtering ?it seems to help. On Monday, September 21, 2015 1:22 PM, Sandra Cheasty via Histonet wrote: Hello all, ? ? ? ? ? ? ? ? Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water bath or our water supply on the stainer. I used sterile gloves, opened a new case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, air-dried and coverslipped them, and the blue dots were there. The only way we got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive. ? ? ? ? ? ? ? ? Thanks for your input, and if you can recommend a different, reasonably priced hematoxylin, that would be awesome. Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones at metropath.com Tue Sep 22 07:59:26 2015 From: mjones at metropath.com (Michael Ann Jones) Date: Tue, 22 Sep 2015 12:59:26 +0000 Subject: [Histonet] Hematoxylin Precipitate In-Reply-To: <1130699889.1214692.1442870261501.JavaMail.yahoo@mail.yahoo.com> References: <4cda87133587e64c965ce6c356d18f59@svm.vetmed.wisc.edu> <1130699889.1214692.1442870261501.JavaMail.yahoo@mail.yahoo.com> Message-ID: Yes, me too. Turquois blue bacteria looking guys. Trying to filter and use within a couple of days. Has anyone mentioned this to their vendor? Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com On 9/21/15, 3:17 PM, "nancy lowen via Histonet" wrote: >Yes, I have had the same experience.Filtering it seems to help. > > > On Monday, September 21, 2015 1:22 PM, Sandra Cheasty via Histonet > wrote: > > > Hello all, > > Has anyone using Richard Allen Hematoxylin-2 noticed an >odd artifact on the slides after using the Hematoxylin for more than a >few days on their stainer? We are seeing small spore or pollen-like blue >dots here and there on the slides. It is not coming from the water bath >or our water supply on the stainer. I used sterile gloves, opened a new >case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on >the stainer, then in DI again, air-dried and coverslipped them, and the >blue dots were there. The only way we got rid of the blue artifact was to >use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > > Thanks for your input, and if you can recommend a >different, reasonably priced hematoxylin, that would be awesome. > >Cheers, > >Sandy > > > >Sandra J. Cheasty, HT (ASCP) > >Histology & Necropsy Supervisor > >UW-Madison, School of Veterinary Medicine > > > > > > > > > > > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alida.bailleul at gmail.com Tue Sep 22 10:27:41 2015 From: alida.bailleul at gmail.com (Alida Bailleul) Date: Tue, 22 Sep 2015 10:27:41 -0500 Subject: [Histonet] Hematoxylin Precipitate In-Reply-To: <4cda87133587e64c965ce6c356d18f59@svm.vetmed.wisc.edu> References: <4cda87133587e64c965ce6c356d18f59@svm.vetmed.wisc.edu> Message-ID: Dear Histonetters, There is some *Denatured Ethanol* bottles in the lab I just started working in (CDA, Fisher brand). Can this denatured ethanol be used for histological processing? I have never used this product, only absolute pure ethanol. Please advise. Thank you very much All the best Alida Bailleul From philip_manfre at merck.com Tue Sep 22 10:49:14 2015 From: philip_manfre at merck.com (Manfre, Philip) Date: Tue, 22 Sep 2015 11:49:14 -0400 Subject: [Histonet] Hematoxylin Precipitate In-Reply-To: References: <4cda87133587e64c965ce6c356d18f59@svm.vetmed.wisc.edu> Message-ID: <558A4571351D0C42BD923F403F4198C40102EF9B2369@USCTMXP51014.merck.com> Denatured alcohol is used all the time. It is generally denatured so people don't drink it. This stems back to a time when the histology lab also made for a great bar and grill. :-) Phil. -----Original Message----- From: Alida Bailleul via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 11:28 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin Precipitate Dear Histonetters, There is some *Denatured Ethanol* bottles in the lab I just started working in (CDA, Fisher brand). Can this denatured ethanol be used for histological processing? I have never used this product, only absolute pure ethanol. Please advise. Thank you very much All the best Alida Bailleul _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From blayjorge at gmail.com Tue Sep 22 11:23:59 2015 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Tue, 22 Sep 2015 12:23:59 -0400 Subject: [Histonet] How deep in skin do hair dyes and shampoos penetrate? Message-ID: Dear Histonetters: The other day, someone ask me in class, how deep in skin do hair dyes and shampoos penetrate? I know tattoos are generally placed in the dermis but I was not sure re. hair dyes and shampoos (incl. those that cause a tingling sensation). If you know, please feel free to email me off the list. Gratefully, Jorge blayjorge at gmail.com Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From thigginsht at msn.com Tue Sep 22 12:25:22 2015 From: thigginsht at msn.com (Tim H) Date: Tue, 22 Sep 2015 12:25:22 -0500 Subject: [Histonet] Hematoxylin Precipitate In-Reply-To: References: Message-ID: You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says. We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been microwave processed. So much tissue washes off in the solutions they should be changed or filtered fairly regularly to try and prevent cross contamination on the slides. You can also try increasing your rinse times and see if that doesn't help as well. Thanks, Tim > > Message: 1 > Date: Mon, 21 Sep 2015 15:14:39 -0500 > From: "Sandra Cheasty" > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > Subject: [Histonet] Hematoxylin Precipitate > Message-ID: <4cda87133587e64c965ce6c356d18f59 at svm.vetmed.wisc.edu> > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water bath or our water supply on the stainer. I used sterile gloves, opened a new case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, air-dried and coverslipped them, and the blue dots were there. The only way we got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > > Thanks for your input, and if you can recommend a different, reasonably priced hematoxylin, that would be awesome. > > Cheers, > > Sandy > > > > Sandra J. Cheasty, HT (ASCP) > > Histology & Necropsy Supervisor > > UW-Madison, School of Veterinary Medicine From liz at premierlab.com Tue Sep 22 12:55:05 2015 From: liz at premierlab.com (Elizabeth Chlipala) Date: Tue, 22 Sep 2015 11:55:05 -0600 Subject: [Histonet] Hematoxylin Precipitate In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02230F9D9510@SBS2K8.premierlab.local> I think you may a have a serious problem if "so much tissue washes off" if that is happening you have problems with tissue adherence. A properly processed and cut section should not wash off the slide, or even a portion of it should not wash off. That would mean that you did not provide to the pathologist what is represented in the block. If that is the case then you would need to filter all solutions on a stainer daily not just the hematoxylin. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 11:25 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin Precipitate You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says. We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been microwave processed. So much tissue washes off in the solutions they should be changed or filtered fairly regularly to try and prevent cross contamination on the slides. You can also try increasing your rinse times and see if that doesn't help as well. Thanks, Tim > > Message: 1 > Date: Mon, 21 Sep 2015 15:14:39 -0500 > From: "Sandra Cheasty" > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > Subject: [Histonet] Hematoxylin Precipitate > Message-ID: <4cda87133587e64c965ce6c356d18f59 at svm.vetmed.wisc.edu> > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water bath or our water supply on the stainer. I used sterile gloves, opened a new case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, air-dried and coverslipped them, and the blue dots were there. The only way we got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > > Thanks for your input, and if you can recommend a different, reasonably priced hematoxylin, that would be awesome. > > Cheers, > > Sandy > > > > Sandra J. Cheasty, HT (ASCP) > > Histology & Necropsy Supervisor > > UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From philip_manfre at merck.com Tue Sep 22 13:26:04 2015 From: philip_manfre at merck.com (Manfre, Philip) Date: Tue, 22 Sep 2015 14:26:04 -0400 Subject: [Histonet] Hematoxylin Precipitate In-Reply-To: <14E2C6176416974295479C64A11CB9AE02230F9D9510@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE02230F9D9510@SBS2K8.premierlab.local> Message-ID: <558A4571351D0C42BD923F403F4198C40102EF9B24B4@USCTMXP51014.merck.com> Wow, I agree with Liz. There should not routinely be "so much tissue washing off". There is a fundamental problem, if this is the case. With regards to hematoxylin, have you tried Gill's Hematoxylin, 1, 2, or 3? These do not need filtering and do not produce a precipitate. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre at merck.com -----Original Message----- From: Elizabeth Chlipala via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 1:55 PM To: Tim H; 'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu) Subject: Re: [Histonet] Hematoxylin Precipitate I think you may a have a serious problem if "so much tissue washes off" if that is happening you have problems with tissue adherence. A properly processed and cut section should not wash off the slide, or even a portion of it should not wash off. That would mean that you did not provide to the pathologist what is represented in the block. If that is the case then you would need to filter all solutions on a stainer daily not just the hematoxylin. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 11:25 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin Precipitate You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says. We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been microwave processed. So much tissue washes off in the solutions they should be changed or filtered fairly regularly to try and prevent cross contamination on the slides. You can also try increasing your rinse times and see if that doesn't help as well. Thanks, Tim > > Message: 1 > Date: Mon, 21 Sep 2015 15:14:39 -0500 > From: "Sandra Cheasty" > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > Subject: [Histonet] Hematoxylin Precipitate > Message-ID: <4cda87133587e64c965ce6c356d18f59 at svm.vetmed.wisc.edu> > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water bath or our water supply on the stainer. I used sterile gloves, opened a new case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, air-dried and coverslipped them, and the blue dots were there. The only way we got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > > Thanks for your input, and if you can recommend a different, reasonably priced hematoxylin, that would be awesome. > > Cheers, > > Sandy > > > > Sandra J. Cheasty, HT (ASCP) > > Histology & Necropsy Supervisor > > UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From gayle.callis at bresnan.net Tue Sep 22 13:27:21 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Tue, 22 Sep 2015 12:27:21 -0600 Subject: [Histonet] Hematoxylin Precipitate In-Reply-To: References: Message-ID: <000601d0f564$523ae2f0$f6b0a8d0$@bresnan.net> Sandy, After years of using Richard Allan's hematoxylin 2 with great success, if we didn't filter daily before use, we had stain precipitate on sections. Some of this comes from the hematoxylin continuing to oxidize in open air, bacteria and other "crud". Tim is absolutely correct ignoring manufacturers no filtering instructions. Being old school, we were taught to faithfully filter any hematoxylin, regardless of progressive or regressive types. If we topped off hematoxylin 2 or used new stock, the stain was filtered into a CLEAN staining container/dish. Keep an extra container around if possible. We used a medium fast filter paper, Whatman 54. I realize this takes time but junk on a slide is NOT good thing, especially after IHC staining and have a photo to show this - the result of being lazy and not filtering the hematoxylin on that particular day. We used a distilled water rinse before hematoxylin2, but DI H2O will be contaminated with cellular debris and last hydrating alcohol carryover. Change DI water frequently if you have many runs in a day. We used 1 minute running tap water rinses after hematoxylin, clearant and bluing. If you are using running water rinses, take a look at the blue ppt in the post hematoxylin container as you don't want that sticking to sections. Non running water rinses should be changed after each H&E run in my opinion. Adequate clean water rinses are important to not have carry over of clearant into bluing reagents or bluing reagent into eosin in order to maintain correct pH for staining. Good luck Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 11:25 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin Precipitate You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says. We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been microwave processed. So much tissue washes off in the solutions they should be changed or filtered fairly regularly to try and prevent cross contamination on the slides. You can also try increasing your rinse times and see if that doesn't help as well. Thanks, Tim > > Message: 1 > Date: Mon, 21 Sep 2015 15:14:39 -0500 > From: "Sandra Cheasty" > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > Subject: [Histonet] Hematoxylin Precipitate > Message-ID: <4cda87133587e64c965ce6c356d18f59 at svm.vetmed.wisc.edu> > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water bath or our water supply on the stainer. I used sterile gloves, opened a new case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, air-dried and coverslipped them, and the blue dots were there. The only way we got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > > Thanks for your input, and if you can recommend a different, reasonably priced hematoxylin, that would be awesome. > > Cheers, > > Sandy > > > > Sandra J. Cheasty, HT (ASCP) > > Histology & Necropsy Supervisor > > UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From elaineahoffman55 at yahoo.com Tue Sep 22 13:44:33 2015 From: elaineahoffman55 at yahoo.com (Elaine allison Hoffman) Date: Tue, 22 Sep 2015 18:44:33 +0000 (UTC) Subject: [Histonet] Webinar presentation hand-outs Message-ID: <1367610594.1779042.1442947473127.JavaMail.yahoo@mail.yahoo.com> Hi all, Just recently joined in with an Advance webinar last week, 9/15/15, Tuesday with speaker, Tim Morken on "Basic Principles of Validation in Histology".? The webinar was very informative and thorough.? The power-point presentation looked to be about 40 pages long.? I'm interested in getting a copy of the the presentation shown during the webinar but don't know how.? There was definitely too much to remember and our lab could really use it ASAP. Wasn't able to write it down fast enough.? I left emails with Advance but no answer back yet.? Any suggestions??? Appreciate any help, thanks.... Elaine Hoffman, HT(ASCP) The Gastroenterology Clinic & Endoscopy Center, Inc.GI Pathology Associates From Timothy.Morken at ucsf.edu Tue Sep 22 14:17:43 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 22 Sep 2015 19:17:43 +0000 Subject: [Histonet] Webinar presentation hand-outs In-Reply-To: <1367610594.1779042.1442947473127.JavaMail.yahoo@mail.yahoo.com> References: <1367610594.1779042.1442947473127.JavaMail.yahoo@mail.yahoo.com> Message-ID: <761E2B5697F795489C8710BCC72141FF603161D9@ex07.net.ucsf.edu> Elaine you can download the powerpoint and all the handouts from the Advance for Laboratories website under Webinars, QA/QC, " Basic Principles of Validation in Histology" Link is below: http://laboratory-manager.advanceweb.com/Webinar/Editorial-Webinars/Basic-Principles-of-Validation-in-Histology.aspx I hope you find it helpful. Contact me directly with any questions or comments. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Elaine allison Hoffman via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 11:45 AM To: Histonet List Subject: [Histonet] Webinar presentation hand-outs Hi all, Just recently joined in with an Advance webinar last week, 9/15/15, Tuesday with speaker, Tim Morken on "Basic Principles of Validation in Histology".? The webinar was very informative and thorough.? The power-point presentation looked to be about 40 pages long.? I'm interested in getting a copy of the the presentation shown during the webinar but don't know how.? There was definitely too much to remember and our lab could really use it ASAP. Wasn't able to write it down fast enough.? I left emails with Advance but no answer back yet.? Any suggestions??? Appreciate any help, thanks.... Elaine Hoffman, HT(ASCP) The Gastroenterology Clinic & Endoscopy Center, Inc.GI Pathology Associates _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thigginsht at msn.com Tue Sep 22 16:04:18 2015 From: thigginsht at msn.com (Tim H) Date: Tue, 22 Sep 2015 16:04:18 -0500 Subject: [Histonet] Hematoxylin Precipitate In-Reply-To: <000601d0f564$523ae2f0$f6b0a8d0$@bresnan.net> References: , <000601d0f564$523ae2f0$f6b0a8d0$@bresnan.net> Message-ID: Liz, Phillip and all that are interested, I take it you have guys never looked at or had someone else examine what is at the bottom of the Hematoxylin filter after you put through a day?s work. There will be tissue particles of tissue along with other contaminates, I am not saying you are going to see a complete LEEP sitting at the bottom but you will have contamination. Liz, maybe the tissue super adheres up there in wacky tabacky country and Phillip sitting in a research facility (are you even involved with processing and staining of slides or just publishing articles?) but in the rest of the United States, I can guarantee you there will be some tissue in the Hematoxylin if you use a traditional dip and dunk system. Liz, you do bring up a good point about having tissue in every container. To a degree you will have tissue in every reagent container. I was assuming and maybe unjustly that most labs are using Good Laboratory Practice and discard their reagents after they have used them for a staining session. This topic was about Hematoxylin Precipitate and "small spore or pollen-like blue dots" which I did not say was tissue, I was passing along some of my 23 years of knowledge. Listen; don't take my word for it. You can have Ventana come out to your facility and filter all your reagents and stains and have them tell you what is in your solutions. Next time just pass along good information and criticize those trying to help! To many people like to make a negative on this site of people trying to truly help. This is why I rarely post on Histonet but instead directly email the person. By no means am I promoting Ventana/Roche products. I am just passing along information that might be helpful. Tim Disclaimer: This information is by no means is meant for any weak stomach individuals or those preparing for the zombie apocalypse. > From: gayle.callis at bresnan.net > To: thigginsht at msn.com; histonet at lists.utsouthwestern.edu > Subject: RE: [Histonet] Hematoxylin Precipitate > Date: Tue, 22 Sep 2015 12:27:21 -0600 > > Sandy, > > After years of using Richard Allan's hematoxylin 2 with great success, if > we didn't filter daily before use, we had stain precipitate on sections. > Some of this comes from the hematoxylin continuing to oxidize in open air, > bacteria and other "crud". Tim is absolutely correct ignoring > manufacturers no filtering instructions. Being old school, we were taught > to faithfully filter any hematoxylin, regardless of progressive or > regressive types. If we topped off hematoxylin 2 or used new stock, the > stain was filtered into a CLEAN staining container/dish. Keep an extra > container around if possible. We used a medium fast filter paper, Whatman > 54. I realize this takes time but junk on a slide is NOT good thing, > especially after IHC staining and have a photo to show this - the result of > being lazy and not filtering the hematoxylin on that particular day. > > We used a distilled water rinse before hematoxylin2, but DI H2O will be > contaminated with cellular debris and last hydrating alcohol carryover. > Change DI water frequently if you have many runs in a day. We used 1 > minute running tap water rinses after hematoxylin, clearant and bluing. If > you are using running water rinses, take a look at the blue ppt in the post > hematoxylin container as you don't want that sticking to sections. Non > running water rinses should be changed after each H&E run in my opinion. > Adequate clean water rinses are important to not have carry over of clearant > into bluing reagents or bluing reagent into eosin in order to maintain > correct pH for staining. > > Good luck > > Gayle M. Callis > HTL/HT/MT(ASCP) > > -----Original Message----- > From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, September 22, 2015 11:25 AM > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Hematoxylin Precipitate > > You should be filtering your Hematoxylin on a daily basis regardless of what > the manufactures says. We use to filter twice a day since we did a > traditional overnight run and then again in the afternoon for specimens that > had been microwave processed. So much tissue washes off in the solutions > they should be changed or filtered fairly regularly to try and prevent cross > contamination on the slides. > > You can also try increasing your rinse times and see if that doesn't help as > well. > > Thanks, > > Tim > > > > Message: 1 > > Date: Mon, 21 Sep 2015 15:14:39 -0500 > > From: "Sandra Cheasty" > > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > > > Subject: [Histonet] Hematoxylin Precipitate > > Message-ID: <4cda87133587e64c965ce6c356d18f59 at svm.vetmed.wisc.edu> > > Content-Type: text/plain; charset="us-ascii" > > > > Hello all, > > > > Has anyone using Richard Allen Hematoxylin-2 noticed an > odd artifact on the slides after using the Hematoxylin for more than a few > days on their stainer? We are seeing small spore or pollen-like blue dots > here and there on the slides. It is not coming from the water bath or our > water supply on the stainer. I used sterile gloves, opened a new case of > slides, dipped them in DI water, then in the RA Hematoxylin 2 on the > stainer, then in DI again, air-dried and coverslipped them, and the blue > dots were there. The only way we got rid of the blue artifact was to use new > RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > > > > Thanks for your input, and if you can recommend a > different, reasonably priced hematoxylin, that would be awesome. > > > > Cheers, > > > > Sandy > > > > > > > > Sandra J. Cheasty, HT (ASCP) > > > > Histology & Necropsy Supervisor > > > > UW-Madison, School of Veterinary Medicine > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From patpxs at gmail.com Tue Sep 22 16:46:11 2015 From: patpxs at gmail.com (P Sicurello) Date: Tue, 22 Sep 2015 14:46:11 -0700 Subject: [Histonet] Elastic Stain on the Benchmark Special Stainer (BMSS)? Message-ID: Hi Netters, Has anyone out in Histoland achieved blackness with the BMSS Elastic stain? All I'm getting are purple elastic fibers. If you have crossed over to the darker side of staining and have a protocol you'd be willing to share, by all means let me know. I want to join the darkside too! At least with the Elastic stain. Thanks in advance, Paula :-) UCSD Health System From liz at premierlab.com Tue Sep 22 17:21:17 2015 From: liz at premierlab.com (Elizabeth Chlipala) Date: Tue, 22 Sep 2015 16:21:17 -0600 Subject: [Histonet] Hematoxylin Precipitate In-Reply-To: References: , <000601d0f564$523ae2f0$f6b0a8d0$@bresnan.net> Message-ID: <14E2C6176416974295479C64A11CB9AE02230F9D951C@SBS2K8.premierlab.local> Tim First of all my comment was not meant to criticize your post and even if you may not think so I was trying to help. I stand by what I said, my comment was to address what I thought was the amount of tissue loss your lab experiences and if I took your comment too literally I apologize but I firmly believe that properly processed and sectioned tissue samples should remain on the slide. I do understand that we will on occasion have loss of tissue from the slides that we cut and stain. We will see a lens floating in one of the alcohols when we stain mouse eyes or a portion of a dermal construct will come off the slides if we have not dried it properly. Most of the tissue loss we experience is due to improperly processed and sectioned samples. We are a small lab and we are GLP compliant. We do not change our H&E staining reagents daily our volume varies depending upon the projects we are working on, but I can tell you that we have looked at H&E staining over time and we have made sure that our reagents are changed appropriately. We do not filter our hematoxylin daily and have not experienced many floaters or carry over or other things on our slides. Liz from wacky tabacky country Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 3:04 PM To: gayle.callis at bresnan.net; Histonet Subject: Re: [Histonet] Hematoxylin Precipitate Liz, Phillip and all that are interested, I take it you have guys never looked at or had someone else examine what is at the bottom of the Hematoxylin filter after you put through a day's work. There will be tissue particles of tissue along with other contaminates, I am not saying you are going to see a complete LEEP sitting at the bottom but you will have contamination. Liz, maybe the tissue super adheres up there in wacky tabacky country and Phillip sitting in a research facility (are you even involved with processing and staining of slides or just publishing articles?) but in the rest of the United States, I can guarantee you there will be some tissue in the Hematoxylin if you use a traditional dip and dunk system. Liz, you do bring up a good point about having tissue in every container. To a degree you will have tissue in every reagent container. I was assuming and maybe unjustly that most labs are using Good Laboratory Practice and discard their reagents after they have used them for a staining session. This topic was about Hematoxylin Precipitate and "small spore or pollen-like blue dots" which I did not say was tissue, I was passing along some of my 23 years of knowledge. Listen; don't take my word for it. You can have Ventana come out to your facility and filter all your reagents and stains and have them tell you what is in your solutions. Next time just pass along good information and criticize those trying to help! To many people like to make a negative on this site of people trying to truly help. This is why I rarely post on Histonet but instead directly email the person. By no means am I promoting Ventana/Roche products. I am just passing along information that might be helpful. Tim Disclaimer: This information is by no means is meant for any weak stomach individuals or those preparing for the zombie apocalypse. > From: gayle.callis at bresnan.net > To: thigginsht at msn.com; histonet at lists.utsouthwestern.edu > Subject: RE: [Histonet] Hematoxylin Precipitate > Date: Tue, 22 Sep 2015 12:27:21 -0600 > > Sandy, > > After years of using Richard Allan's hematoxylin 2 with great success, if > we didn't filter daily before use, we had stain precipitate on sections. > Some of this comes from the hematoxylin continuing to oxidize in open air, > bacteria and other "crud". Tim is absolutely correct ignoring > manufacturers no filtering instructions. Being old school, we were taught > to faithfully filter any hematoxylin, regardless of progressive or > regressive types. If we topped off hematoxylin 2 or used new stock, the > stain was filtered into a CLEAN staining container/dish. Keep an extra > container around if possible. We used a medium fast filter paper, Whatman > 54. I realize this takes time but junk on a slide is NOT good thing, > especially after IHC staining and have a photo to show this - the result of > being lazy and not filtering the hematoxylin on that particular day. > > We used a distilled water rinse before hematoxylin2, but DI H2O will > be contaminated with cellular debris and last hydrating alcohol carryover. > Change DI water frequently if you have many runs in a day. We used 1 > minute running tap water rinses after hematoxylin, clearant and bluing. If > you are using running water rinses, take a look at the blue ppt in the post > hematoxylin container as you don't want that sticking to sections. Non > running water rinses should be changed after each H&E run in my opinion. > Adequate clean water rinses are important to not have carry over of > clearant into bluing reagents or bluing reagent into eosin in order to maintain > correct pH for staining. > > Good luck > > Gayle M. Callis > HTL/HT/MT(ASCP) > > -----Original Message----- > From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, September 22, 2015 11:25 AM > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Hematoxylin Precipitate > > You should be filtering your Hematoxylin on a daily basis regardless > of what the manufactures says. We use to filter twice a day since we > did a traditional overnight run and then again in the afternoon for > specimens that had been microwave processed. So much tissue washes > off in the solutions they should be changed or filtered fairly > regularly to try and prevent cross contamination on the slides. > > You can also try increasing your rinse times and see if that doesn't > help as well. > > Thanks, > > Tim > > > > Message: 1 > > Date: Mon, 21 Sep 2015 15:14:39 -0500 > > From: "Sandra Cheasty" > > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > > > Subject: [Histonet] Hematoxylin Precipitate > > Message-ID: <4cda87133587e64c965ce6c356d18f59 at svm.vetmed.wisc.edu> > > Content-Type: text/plain; charset="us-ascii" > > > > Hello all, > > > > Has anyone using Richard Allen Hematoxylin-2 noticed > > an > odd artifact on the slides after using the Hematoxylin for more than a > few days on their stainer? We are seeing small spore or pollen-like > blue dots here and there on the slides. It is not coming from the > water bath or our water supply on the stainer. I used sterile gloves, > opened a new case of slides, dipped them in DI water, then in the RA > Hematoxylin 2 on the stainer, then in DI again, air-dried and > coverslipped them, and the blue dots were there. The only way we got > rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > > > > Thanks for your input, and if you can recommend a > different, reasonably priced hematoxylin, that would be awesome. > > > > Cheers, > > > > Sandy > > > > > > > > Sandra J. Cheasty, HT (ASCP) > > > > Histology & Necropsy Supervisor > > > > UW-Madison, School of Veterinary Medicine > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patrick.lewis at seattlechildrens.org Tue Sep 22 17:36:09 2015 From: patrick.lewis at seattlechildrens.org (Lewis, Patrick) Date: Tue, 22 Sep 2015 22:36:09 +0000 Subject: [Histonet] Quick H202 quenching question. Message-ID: <3903BE18914F4440834F0E620415FFCA3CBA2228@PPWEXD01d.childrens.sea.kids> If I have an IHC where I am staining 2 slides from the same block, one with one primary antibody and the other with a different primary antibody. And one antibody's slides have high nonspecific background, but the other antibody's slides have no background, Can I deduce that the background staining is not caused by insufficient H202 quenching/blocking? All the steps were the same, except for the primary antibody/secondary antibody. Also, they did have different epitope retrievals. But the wash buffers/blocking buffers/and substrate were the same. I am fairly confident that my background problems are related to this particular primary antibody, and not the actual quenching/blocknig from my IHC protocol, but I thought I'd check with you guys. Patrick. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From gayle.callis at bresnan.net Tue Sep 22 17:59:55 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Tue, 22 Sep 2015 16:59:55 -0600 Subject: [Histonet] Hematoxylin Precipitate and filtering Gill formulations Message-ID: <000301d0f58a$663f49a0$32bddce0$@bresnan.net> Yes, I have used Gill 1, 2 and 3 even in the early days of buying these formulations from a vendor, and always filtered them before using. Old school habits never changed.............. Gayle Callis -----Original Message----- From: Manfre, Philip via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 12:26 PM To: Elizabeth Chlipala ; Tim H Cc: 'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu) Subject: Re: [Histonet] Hematoxylin Precipitate Wow, I agree with Liz. There should not routinely be "so much tissue washing off". There is a fundamental problem, if this is the case. With regards to hematoxylin, have you tried Gill's Hematoxylin, 1, 2, or 3? These do not need filtering and do not produce a precipitate. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre at merck.com From philip_manfre at merck.com Wed Sep 23 06:55:59 2015 From: philip_manfre at merck.com (Manfre, Philip) Date: Wed, 23 Sep 2015 07:55:59 -0400 Subject: [Histonet] Hematoxylin Precipitate In-Reply-To: References: , <000601d0f564$523ae2f0$f6b0a8d0$@bresnan.net> Message-ID: <558A4571351D0C42BD923F403F4198C40102EF9B26BF@USCTMXP51014.merck.com> Well Tim, In the 31 years I have been in histology, I obviously have done all lab work with my eyes closed, never looking at the equipment or solutions I have been using. I guess my stint in a high throughput contract lab taught me nothing nor did my 25 + years in big Pharma, notorious for hiring every hack who knows what a microscope slide is. You suggested that I sit around getting published... I am published, BTW, but that accounts for 0.01% of my experiences in the lab, yes, in the lab processing, embedding, sectioning (quite good at that), cryosectioning, performing and troubleshooting IHC and histochemical stains. Something I may have learned, and maybe you will when you catch up, is not assume you know what someone else's work experiences may have been or are. Histology occurs in many environments with differing equipment, reagents, processing styles, etc. Some are better than others. I guess our inadequate processing methods fall short, leaving far too much tissue on the slide where it belongs, and thus we don't have big tissue globs clogging everything, nor do we see precipitate with the hematoxylin used. Traditional formulations of hematoxylin, sure, absolutely filter. For anyone who benefits from filtering, why not continue. No harm will come. I was merely trying to advise someone who seemed to have an issue I thought I could help with. Now I realize all issues should be relegated to Tim, the Charles Churukian of our times. Look him up, if you're stumped by the reference. Maybe I'll head for Wacky Tabacky land for a vacation. It is quite beautiful there... Phil. -----Original Message----- From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 5:04 PM To: gayle.callis at bresnan.net; Histonet Subject: Re: [Histonet] Hematoxylin Precipitate Liz, Phillip and all that are interested, I take it you have guys never looked at or had someone else examine what is at the bottom of the Hematoxylin filter after you put through a day's work. There will be tissue particles of tissue along with other contaminates, I am not saying you are going to see a complete LEEP sitting at the bottom but you will have contamination. Liz, maybe the tissue super adheres up there in wacky tabacky country and Phillip sitting in a research facility (are you even involved with processing and staining of slides or just publishing articles?) but in the rest of the United States, I can guarantee you there will be some tissue in the Hematoxylin if you use a traditional dip and dunk system. Liz, you do bring up a good point about having tissue in every container. To a degree you will have tissue in every reagent container. I was assuming and maybe unjustly that most labs are using Good Laboratory Practice and discard their reagents after they have used them for a staining session. This topic was about Hematoxylin Precipitate and "small spore or pollen-like blue dots" which I did not say was tissue, I was passing along some of my 23 years of knowledge. Listen; don't take my word for it. You can have Ventana come out to your facility and filter all your reagents and stains and have them tell you what is in your solutions. Next time just pass along good information and criticize those trying to help! To many people like to make a negative on this site of people trying to truly help. This is why I rarely post on Histonet but instead directly email the person. By no means am I promoting Ventana/Roche products. I am just passing along information that might be helpful. Tim Disclaimer: This information is by no means is meant for any weak stomach individuals or those preparing for the zombie apocalypse. > From: gayle.callis at bresnan.net > To: thigginsht at msn.com; histonet at lists.utsouthwestern.edu > Subject: RE: [Histonet] Hematoxylin Precipitate > Date: Tue, 22 Sep 2015 12:27:21 -0600 > > Sandy, > > After years of using Richard Allan's hematoxylin 2 with great success, if > we didn't filter daily before use, we had stain precipitate on sections. > Some of this comes from the hematoxylin continuing to oxidize in open air, > bacteria and other "crud". Tim is absolutely correct ignoring > manufacturers no filtering instructions. Being old school, we were taught > to faithfully filter any hematoxylin, regardless of progressive or > regressive types. If we topped off hematoxylin 2 or used new stock, the > stain was filtered into a CLEAN staining container/dish. Keep an extra > container around if possible. We used a medium fast filter paper, Whatman > 54. I realize this takes time but junk on a slide is NOT good thing, > especially after IHC staining and have a photo to show this - the result of > being lazy and not filtering the hematoxylin on that particular day. > > We used a distilled water rinse before hematoxylin2, but DI H2O will be > contaminated with cellular debris and last hydrating alcohol carryover. > Change DI water frequently if you have many runs in a day. We used 1 > minute running tap water rinses after hematoxylin, clearant and bluing. If > you are using running water rinses, take a look at the blue ppt in the post > hematoxylin container as you don't want that sticking to sections. Non > running water rinses should be changed after each H&E run in my opinion. > Adequate clean water rinses are important to not have carry over of clearant > into bluing reagents or bluing reagent into eosin in order to maintain > correct pH for staining. > > Good luck > > Gayle M. Callis > HTL/HT/MT(ASCP) > > -----Original Message----- > From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, September 22, 2015 11:25 AM > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Hematoxylin Precipitate > > You should be filtering your Hematoxylin on a daily basis regardless of what > the manufactures says. We use to filter twice a day since we did a > traditional overnight run and then again in the afternoon for specimens that > had been microwave processed. So much tissue washes off in the solutions > they should be changed or filtered fairly regularly to try and prevent cross > contamination on the slides. > > You can also try increasing your rinse times and see if that doesn't help as > well. > > Thanks, > > Tim > > > > Message: 1 > > Date: Mon, 21 Sep 2015 15:14:39 -0500 > > From: "Sandra Cheasty" > > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > > > Subject: [Histonet] Hematoxylin Precipitate > > Message-ID: <4cda87133587e64c965ce6c356d18f59 at svm.vetmed.wisc.edu> > > Content-Type: text/plain; charset="us-ascii" > > > > Hello all, > > > > Has anyone using Richard Allen Hematoxylin-2 noticed an > odd artifact on the slides after using the Hematoxylin for more than a few > days on their stainer? We are seeing small spore or pollen-like blue dots > here and there on the slides. It is not coming from the water bath or our > water supply on the stainer. I used sterile gloves, opened a new case of > slides, dipped them in DI water, then in the RA Hematoxylin 2 on the > stainer, then in DI again, air-dried and coverslipped them, and the blue > dots were there. The only way we got rid of the blue artifact was to use new > RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > > > > Thanks for your input, and if you can recommend a > different, reasonably priced hematoxylin, that would be awesome. > > > > Cheers, > > > > Sandy > > > > > > > > Sandra J. Cheasty, HT (ASCP) > > > > Histology & Necropsy Supervisor > > > > UW-Madison, School of Veterinary Medicine > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Nancy_Schmitt at pa-ucl.com Wed Sep 23 10:33:30 2015 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Wed, 23 Sep 2015 15:33:30 +0000 Subject: [Histonet] Cryostate decon ANP.23410 Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C360115A4B354@PEITHA.wad.pa-ucl.com> Good Morning- Defrost and decontaminate with TB disinfectant weekly if used daily. How are you best managing this and what are you using to decontaminate for TB? Thank you Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From mjones at metropath.com Wed Sep 23 11:04:46 2015 From: mjones at metropath.com (Michael Ann Jones) Date: Wed, 23 Sep 2015 16:04:46 +0000 Subject: [Histonet] Cryostate decon ANP.23410 Message-ID: We do maybe 11 frozens/month and so decon/defrost quarterly. Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com On 9/23/15, 9:33 AM, "Nancy Schmitt via Histonet" wrote: >Good Morning- >Defrost and decontaminate with TB disinfectant weekly if used daily. How >are you best managing this and what are you using to decontaminate for TB? >Thank you > >Nancy Schmitt MLT, HT(ASCP) >United Clinical Laboratories >Dubuque, IA 52001 > > > > > >NOTICE: This email may contain legally privileged information. The >information >is for the use of only the intended recipient(s) even if addressed >incorrectly. If you are not the intended recipient, please notify the >sender >that you have received it in error and then delete it along with any >attachments. Thank you. > > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Wed Sep 23 12:16:55 2015 From: rsrichmond at gmail.com (Bob Richmond) Date: Wed, 23 Sep 2015 13:16:55 -0400 Subject: [Histonet] Hematoxylin Precipitate Message-ID: The alum hematoxylins I've worked with required daily filtration. An iridescent scum on top of the hematoxylin means you're overdue to filter. When you brew a fresh batch of alum hematoxylin, it's a light yellow color. As it oxidizes (slowly if you depend on atmospheric oxygen, instantly if you add an oxidant such sodium iodate or mercuric oxide) it turns into deep purple hematein (say hee-muh-TEE-in, not the same as hematin) which is what you stain with. Hematein oxidizes further to filterable crud. Whatman No.1 filter paper is fast and easy to get, and is usually preferred. Sometimes Good Management Practice requires substituting a paper towel, which works reasonably well. You young whippersnappers shouldn't need to be told this by a 76 year old pathologist! Bob Richmond Samurai Pathologist Maryville TN From Fai.Chung at cshs.org Wed Sep 23 12:53:48 2015 From: Fai.Chung at cshs.org (Chung, Fai) Date: Wed, 23 Sep 2015 17:53:48 +0000 Subject: [Histonet] Ventana Discovery Message-ID: Ventana is selling their Discovery instrument for research use only. I am wondering if anyone know if a complete full validation is done on the instrument, can we use it on clinical cases as a "laboratory developed test". For antibody classified as RUO, we are able to use it on patient slide as a "laboratory developed" test as long as we do a complete full validation. Thanks. Fai -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Wednesday, September 23, 2015 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 142, Issue 19 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Hematoxylin Precipitate (Tim H) 2. Re: Hematoxylin Precipitate (Elizabeth Chlipala) 3. Re: Hematoxylin Precipitate (Manfre, Philip) 4. Re: Hematoxylin Precipitate (Gayle Callis) 5. Webinar presentation hand-outs (Elaine allison Hoffman) 6. Re: Webinar presentation hand-outs (Morken, Timothy) 7. Re: Hematoxylin Precipitate (Tim H) 8. Elastic Stain on the Benchmark Special Stainer (BMSS)? (P Sicurello) 9. Re: Hematoxylin Precipitate (Elizabeth Chlipala) 10. Quick H202 quenching question. (Lewis, Patrick) 11. Re: Hematoxylin Precipitate and filtering Gill formulations (Gayle Callis) 12. Re: Hematoxylin Precipitate (Manfre, Philip) 13. Cryostate decon ANP.23410 (Nancy Schmitt) 14. Re: Cryostate decon ANP.23410 (Michael Ann Jones) ---------------------------------------------------------------------- Message: 1 Date: Tue, 22 Sep 2015 12:25:22 -0500 From: Tim H To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Hematoxylin Precipitate Message-ID: Content-Type: text/plain; charset="iso-8859-1" You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says. We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been microwave processed. So much tissue washes off in the solutions they should be changed or filtered fairly regularly to try and prevent cross contamination on the slides. You can also try increasing your rinse times and see if that doesn't help as well. Thanks, Tim > > Message: 1 > Date: Mon, 21 Sep 2015 15:14:39 -0500 > From: "Sandra Cheasty" > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > Subject: [Histonet] Hematoxylin Precipitate > Message-ID: <4cda87133587e64c965ce6c356d18f59 at svm.vetmed.wisc.edu> > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water bath or our water supply on the stainer. I used sterile gloves, opened a new case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, air-dried and coverslipped them, and the blue dots were there. The only way we got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > > Thanks for your input, and if you can recommend a different, reasonably priced hematoxylin, that would be awesome. > > Cheers, > > Sandy > > > > Sandra J. Cheasty, HT (ASCP) > > Histology & Necropsy Supervisor > > UW-Madison, School of Veterinary Medicine ------------------------------ Message: 2 Date: Tue, 22 Sep 2015 11:55:05 -0600 From: Elizabeth Chlipala To: Tim H , "'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)" Subject: Re: [Histonet] Hematoxylin Precipitate Message-ID: <14E2C6176416974295479C64A11CB9AE02230F9D9510 at SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" I think you may a have a serious problem if "so much tissue washes off" if that is happening you have problems with tissue adherence. A properly processed and cut section should not wash off the slide, or even a portion of it should not wash off. That would mean that you did not provide to the pathologist what is represented in the block. If that is the case then you would need to filter all solutions on a stainer daily not just the hematoxylin. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 11:25 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin Precipitate You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says. We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been microwave processed. So much tissue washes off in the solutions they should be changed or filtered fairly regularly to try and prevent cross contamination on the slides. You can also try increasing your rinse times and see if that doesn't help as well. Thanks, Tim > > Message: 1 > Date: Mon, 21 Sep 2015 15:14:39 -0500 > From: "Sandra Cheasty" > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > Subject: [Histonet] Hematoxylin Precipitate > Message-ID: <4cda87133587e64c965ce6c356d18f59 at svm.vetmed.wisc.edu> > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water bath or our water supply on the stainer. I used sterile gloves, opened a new case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, air-dried and coverslipped them, and the blue dots were there. The only way we got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > > Thanks for your input, and if you can recommend a different, reasonably priced hematoxylin, that would be awesome. > > Cheers, > > Sandy > > > > Sandra J. Cheasty, HT (ASCP) > > Histology & Necropsy Supervisor > > UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 22 Sep 2015 14:26:04 -0400 From: "Manfre, Philip" To: Elizabeth Chlipala , Tim H Cc: "'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)" Subject: Re: [Histonet] Hematoxylin Precipitate Message-ID: <558A4571351D0C42BD923F403F4198C40102EF9B24B4 at USCTMXP51014.merck.com> Content-Type: text/plain; charset="us-ascii" Wow, I agree with Liz. There should not routinely be "so much tissue washing off". There is a fundamental problem, if this is the case. With regards to hematoxylin, have you tried Gill's Hematoxylin, 1, 2, or 3? These do not need filtering and do not produce a precipitate. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre at merck.com -----Original Message----- From: Elizabeth Chlipala via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 1:55 PM To: Tim H; 'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu) Subject: Re: [Histonet] Hematoxylin Precipitate I think you may a have a serious problem if "so much tissue washes off" if that is happening you have problems with tissue adherence. A properly processed and cut section should not wash off the slide, or even a portion of it should not wash off. That would mean that you did not provide to the pathologist what is represented in the block. If that is the case then you would need to filter all solutions on a stainer daily not just the hematoxylin. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 11:25 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin Precipitate You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says. We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been microwave processed. So much tissue washes off in the solutions they should be changed or filtered fairly regularly to try and prevent cross contamination on the slides. You can also try increasing your rinse times and see if that doesn't help as well. Thanks, Tim > > Message: 1 > Date: Mon, 21 Sep 2015 15:14:39 -0500 > From: "Sandra Cheasty" > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > Subject: [Histonet] Hematoxylin Precipitate > Message-ID: <4cda87133587e64c965ce6c356d18f59 at svm.vetmed.wisc.edu> > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water bath or our water supply on the stainer. I used sterile gloves, opened a new case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, air-dried and coverslipped them, and the blue dots were there. The only way we got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > > Thanks for your input, and if you can recommend a different, reasonably priced hematoxylin, that would be awesome. > > Cheers, > > Sandy > > > > Sandra J. Cheasty, HT (ASCP) > > Histology & Necropsy Supervisor > > UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 4 Date: Tue, 22 Sep 2015 12:27:21 -0600 From: "Gayle Callis" To: "'Tim H'" , "Histonet" Subject: Re: [Histonet] Hematoxylin Precipitate Message-ID: <000601d0f564$523ae2f0$f6b0a8d0$@bresnan.net> Content-Type: text/plain; charset="us-ascii" Sandy, After years of using Richard Allan's hematoxylin 2 with great success, if we didn't filter daily before use, we had stain precipitate on sections. Some of this comes from the hematoxylin continuing to oxidize in open air, bacteria and other "crud". Tim is absolutely correct ignoring manufacturers no filtering instructions. Being old school, we were taught to faithfully filter any hematoxylin, regardless of progressive or regressive types. If we topped off hematoxylin 2 or used new stock, the stain was filtered into a CLEAN staining container/dish. Keep an extra container around if possible. We used a medium fast filter paper, Whatman 54. I realize this takes time but junk on a slide is NOT good thing, especially after IHC staining and have a photo to show this - the result of being lazy and not filtering the hematoxylin on that particular day. We used a distilled water rinse before hematoxylin2, but DI H2O will be contaminated with cellular debris and last hydrating alcohol carryover. Change DI water frequently if you have many runs in a day. We used 1 minute running tap water rinses after hematoxylin, clearant and bluing. If you are using running water rinses, take a look at the blue ppt in the post hematoxylin container as you don't want that sticking to sections. Non running water rinses should be changed after each H&E run in my opinion. Adequate clean water rinses are important to not have carry over of clearant into bluing reagents or bluing reagent into eosin in order to maintain correct pH for staining. Good luck Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 11:25 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin Precipitate You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says. We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been microwave processed. So much tissue washes off in the solutions they should be changed or filtered fairly regularly to try and prevent cross contamination on the slides. You can also try increasing your rinse times and see if that doesn't help as well. Thanks, Tim > > Message: 1 > Date: Mon, 21 Sep 2015 15:14:39 -0500 > From: "Sandra Cheasty" > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > Subject: [Histonet] Hematoxylin Precipitate > Message-ID: <4cda87133587e64c965ce6c356d18f59 at svm.vetmed.wisc.edu> > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water bath or our water supply on the stainer. I used sterile gloves, opened a new case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, air-dried and coverslipped them, and the blue dots were there. The only way we got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > > Thanks for your input, and if you can recommend a different, reasonably priced hematoxylin, that would be awesome. > > Cheers, > > Sandy > > > > Sandra J. Cheasty, HT (ASCP) > > Histology & Necropsy Supervisor > > UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 22 Sep 2015 18:44:33 +0000 (UTC) From: Elaine allison Hoffman To: Histonet List Subject: [Histonet] Webinar presentation hand-outs Message-ID: <1367610594.1779042.1442947473127.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Hi all, Just recently joined in with an Advance webinar last week, 9/15/15, Tuesday with speaker, Tim Morken on "Basic Principles of Validation in Histology".? The webinar was very informative and thorough.? The power-point presentation looked to be about 40 pages long.? I'm interested in getting a copy of the the presentation shown during the webinar but don't know how.? There was definitely too much to remember and our lab could really use it ASAP. Wasn't able to write it down fast enough.? I left emails with Advance but no answer back yet.? Any suggestions??? Appreciate any help, thanks.... Elaine Hoffman, HT(ASCP) The Gastroenterology Clinic & Endoscopy Center, Inc.GI Pathology Associates ------------------------------ Message: 6 Date: Tue, 22 Sep 2015 19:17:43 +0000 From: "Morken, Timothy" To: Elaine allison Hoffman Cc: Histonet Subject: Re: [Histonet] Webinar presentation hand-outs Message-ID: <761E2B5697F795489C8710BCC72141FF603161D9 at ex07.net.ucsf.edu> Content-Type: text/plain; charset="utf-8" Elaine you can download the powerpoint and all the handouts from the Advance for Laboratories website under Webinars, QA/QC, " Basic Principles of Validation in Histology" Link is below: http://laboratory-manager.advanceweb.com/Webinar/Editorial-Webinars/Basic-Principles-of-Validation-in-Histology.aspx I hope you find it helpful. Contact me directly with any questions or comments. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Elaine allison Hoffman via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 11:45 AM To: Histonet List Subject: [Histonet] Webinar presentation hand-outs Hi all, Just recently joined in with an Advance webinar last week, 9/15/15, Tuesday with speaker, Tim Morken on "Basic Principles of Validation in Histology".? The webinar was very informative and thorough.? The power-point presentation looked to be about 40 pages long.? I'm interested in getting a copy of the the presentation shown during the webinar but don't know how.? There was definitely too much to remember and our lab could really use it ASAP. Wasn't able to write it down fast enough.? I left emails with Advance but no answer back yet.? Any suggestions??? Appreciate any help, thanks.... Elaine Hoffman, HT(ASCP) The Gastroenterology Clinic & Endoscopy Center, Inc.GI Pathology Associates _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 22 Sep 2015 16:04:18 -0500 From: Tim H To: "gayle.callis at bresnan.net" , Histonet Subject: Re: [Histonet] Hematoxylin Precipitate Message-ID: Content-Type: text/plain; charset="Windows-1252" Liz, Phillip and all that are interested, I take it you have guys never looked at or had someone else examine what is at the bottom of the Hematoxylin filter after you put through a day?s work. There will be tissue particles of tissue along with other contaminates, I am not saying you are going to see a complete LEEP sitting at the bottom but you will have contamination. Liz, maybe the tissue super adheres up there in wacky tabacky country and Phillip sitting in a research facility (are you even involved with processing and staining of slides or just publishing articles?) but in the rest of the United States, I can guarantee you there will be some tissue in the Hematoxylin if you use a traditional dip and dunk system. Liz, you do bring up a good point about having tissue in every container. To a degree you will have tissue in every reagent container. I was assuming and maybe unjustly that most labs are using Good Laboratory Practice and discard their reagents after they have used them for a staining session. This topic was about Hematoxylin Precipitate and "small spore or pollen-like blue dots" which I did not say was tissue, I was passing along some of my 23 years of knowledge. Listen; don't take my word for it. You can have Ventana come out to your facility and filter all your reagents and stains and have them tell you what is in your solutions. Next time just pass along good information and criticize those trying to help! To many people like to make a negative on this site of people trying to truly help. This is why I rarely post on Histonet but instead directly email the person. By no means am I promoting Ventana/Roche products. I am just passing along information that might be helpful. Tim Disclaimer: This information is by no means is meant for any weak stomach individuals or those preparing for the zombie apocalypse. > From: gayle.callis at bresnan.net > To: thigginsht at msn.com; histonet at lists.utsouthwestern.edu > Subject: RE: [Histonet] Hematoxylin Precipitate > Date: Tue, 22 Sep 2015 12:27:21 -0600 > > Sandy, > > After years of using Richard Allan's hematoxylin 2 with great success, if > we didn't filter daily before use, we had stain precipitate on sections. > Some of this comes from the hematoxylin continuing to oxidize in open air, > bacteria and other "crud". Tim is absolutely correct ignoring > manufacturers no filtering instructions. Being old school, we were taught > to faithfully filter any hematoxylin, regardless of progressive or > regressive types. If we topped off hematoxylin 2 or used new stock, the > stain was filtered into a CLEAN staining container/dish. Keep an extra > container around if possible. We used a medium fast filter paper, Whatman > 54. I realize this takes time but junk on a slide is NOT good thing, > especially after IHC staining and have a photo to show this - the result of > being lazy and not filtering the hematoxylin on that particular day. > > We used a distilled water rinse before hematoxylin2, but DI H2O will be > contaminated with cellular debris and last hydrating alcohol carryover. > Change DI water frequently if you have many runs in a day. We used 1 > minute running tap water rinses after hematoxylin, clearant and bluing. If > you are using running water rinses, take a look at the blue ppt in the post > hematoxylin container as you don't want that sticking to sections. Non > running water rinses should be changed after each H&E run in my opinion. > Adequate clean water rinses are important to not have carry over of clearant > into bluing reagents or bluing reagent into eosin in order to maintain > correct pH for staining. > > Good luck > > Gayle M. Callis > HTL/HT/MT(ASCP) > > -----Original Message----- > From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, September 22, 2015 11:25 AM > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Hematoxylin Precipitate > > You should be filtering your Hematoxylin on a daily basis regardless of what > the manufactures says. We use to filter twice a day since we did a > traditional overnight run and then again in the afternoon for specimens that > had been microwave processed. So much tissue washes off in the solutions > they should be changed or filtered fairly regularly to try and prevent cross > contamination on the slides. > > You can also try increasing your rinse times and see if that doesn't help as > well. > > Thanks, > > Tim > > > > Message: 1 > > Date: Mon, 21 Sep 2015 15:14:39 -0500 > > From: "Sandra Cheasty" > > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > > > Subject: [Histonet] Hematoxylin Precipitate > > Message-ID: <4cda87133587e64c965ce6c356d18f59 at svm.vetmed.wisc.edu> > > Content-Type: text/plain; charset="us-ascii" > > > > Hello all, > > > > Has anyone using Richard Allen Hematoxylin-2 noticed an > odd artifact on the slides after using the Hematoxylin for more than a few > days on their stainer? We are seeing small spore or pollen-like blue dots > here and there on the slides. It is not coming from the water bath or our > water supply on the stainer. I used sterile gloves, opened a new case of > slides, dipped them in DI water, then in the RA Hematoxylin 2 on the > stainer, then in DI again, air-dried and coverslipped them, and the blue > dots were there. The only way we got rid of the blue artifact was to use new > RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > > > > Thanks for your input, and if you can recommend a > different, reasonably priced hematoxylin, that would be awesome. > > > > Cheers, > > > > Sandy > > > > > > > > Sandra J. Cheasty, HT (ASCP) > > > > Histology & Necropsy Supervisor > > > > UW-Madison, School of Veterinary Medicine > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Tue, 22 Sep 2015 14:46:11 -0700 From: P Sicurello To: HistoNet Subject: [Histonet] Elastic Stain on the Benchmark Special Stainer (BMSS)? Message-ID: Content-Type: text/plain; charset=UTF-8 Hi Netters, Has anyone out in Histoland achieved blackness with the BMSS Elastic stain? All I'm getting are purple elastic fibers. If you have crossed over to the darker side of staining and have a protocol you'd be willing to share, by all means let me know. I want to join the darkside too! At least with the Elastic stain. Thanks in advance, Paula :-) UCSD Health System ------------------------------ Message: 9 Date: Tue, 22 Sep 2015 16:21:17 -0600 From: Elizabeth Chlipala To: Tim H Cc: "'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)" Subject: Re: [Histonet] Hematoxylin Precipitate Message-ID: <14E2C6176416974295479C64A11CB9AE02230F9D951C at SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Tim First of all my comment was not meant to criticize your post and even if you may not think so I was trying to help. I stand by what I said, my comment was to address what I thought was the amount of tissue loss your lab experiences and if I took your comment too literally I apologize but I firmly believe that properly processed and sectioned tissue samples should remain on the slide. I do understand that we will on occasion have loss of tissue from the slides that we cut and stain. We will see a lens floating in one of the alcohols when we stain mouse eyes or a portion of a dermal construct will come off the slides if we have not dried it properly. Most of the tissue loss we experience is due to improperly processed and sectioned samples. We are a small lab and we are GLP compliant. We do not change our H&E staining reagents daily our volume varies depending upon the projects we are working on, but I can tell you that we have looked at H&E staining over time and we have made sure that our reagents are changed appropriately. We do not filter our hematoxylin daily and have not experienced many floaters or carry over or other things on our slides. Liz from wacky tabacky country Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 3:04 PM To: gayle.callis at bresnan.net; Histonet Subject: Re: [Histonet] Hematoxylin Precipitate Liz, Phillip and all that are interested, I take it you have guys never looked at or had someone else examine what is at the bottom of the Hematoxylin filter after you put through a day's work. There will be tissue particles of tissue along with other contaminates, I am not saying you are going to see a complete LEEP sitting at the bottom but you will have contamination. Liz, maybe the tissue super adheres up there in wacky tabacky country and Phillip sitting in a research facility (are you even involved with processing and staining of slides or just publishing articles?) but in the rest of the United States, I can guarantee you there will be some tissue in the Hematoxylin if you use a traditional dip and dunk system. Liz, you do bring up a good point about having tissue in every container. To a degree you will have tissue in every reagent container. I was assuming and maybe unjustly that most labs are using Good Laboratory Practice and discard their reagents after they have used them for a staining session. This topic was about Hematoxylin Precipitate and "small spore or pollen-like blue dots" which I did not say was tissue, I was passing along some of my 23 years of knowledge. Listen; don't take my word for it. You can have Ventana come out to your facility and filter all your reagents and stains and have them tell you what is in your solutions. Next time just pass along good information and criticize those trying to help! To many people like to make a negative on this site of people trying to truly help. This is why I rarely post on Histonet but instead directly email the person. By no means am I promoting Ventana/Roche products. I am just passing along information that might be helpful. Tim Disclaimer: This information is by no means is meant for any weak stomach individuals or those preparing for the zombie apocalypse. > From: gayle.callis at bresnan.net > To: thigginsht at msn.com; histonet at lists.utsouthwestern.edu > Subject: RE: [Histonet] Hematoxylin Precipitate > Date: Tue, 22 Sep 2015 12:27:21 -0600 > > Sandy, > > After years of using Richard Allan's hematoxylin 2 with great success, if > we didn't filter daily before use, we had stain precipitate on sections. > Some of this comes from the hematoxylin continuing to oxidize in open air, > bacteria and other "crud". Tim is absolutely correct ignoring > manufacturers no filtering instructions. Being old school, we were taught > to faithfully filter any hematoxylin, regardless of progressive or > regressive types. If we topped off hematoxylin 2 or used new stock, the > stain was filtered into a CLEAN staining container/dish. Keep an extra > container around if possible. We used a medium fast filter paper, Whatman > 54. I realize this takes time but junk on a slide is NOT good thing, > especially after IHC staining and have a photo to show this - the result of > being lazy and not filtering the hematoxylin on that particular day. > > We used a distilled water rinse before hematoxylin2, but DI H2O will > be contaminated with cellular debris and last hydrating alcohol carryover. > Change DI water frequently if you have many runs in a day. We used 1 > minute running tap water rinses after hematoxylin, clearant and bluing. If > you are using running water rinses, take a look at the blue ppt in the post > hematoxylin container as you don't want that sticking to sections. Non > running water rinses should be changed after each H&E run in my opinion. > Adequate clean water rinses are important to not have carry over of > clearant into bluing reagents or bluing reagent into eosin in order to maintain > correct pH for staining. > > Good luck > > Gayle M. Callis > HTL/HT/MT(ASCP) > > -----Original Message----- > From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, September 22, 2015 11:25 AM > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Hematoxylin Precipitate > > You should be filtering your Hematoxylin on a daily basis regardless > of what the manufactures says. We use to filter twice a day since we > did a traditional overnight run and then again in the afternoon for > specimens that had been microwave processed. So much tissue washes > off in the solutions they should be changed or filtered fairly > regularly to try and prevent cross contamination on the slides. > > You can also try increasing your rinse times and see if that doesn't > help as well. > > Thanks, > > Tim > > > > Message: 1 > > Date: Mon, 21 Sep 2015 15:14:39 -0500 > > From: "Sandra Cheasty" > > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > > > Subject: [Histonet] Hematoxylin Precipitate > > Message-ID: <4cda87133587e64c965ce6c356d18f59 at svm.vetmed.wisc.edu> > > Content-Type: text/plain; charset="us-ascii" > > > > Hello all, > > > > Has anyone using Richard Allen Hematoxylin-2 noticed > > an > odd artifact on the slides after using the Hematoxylin for more than a > few days on their stainer? We are seeing small spore or pollen-like > blue dots here and there on the slides. It is not coming from the > water bath or our water supply on the stainer. I used sterile gloves, > opened a new case of slides, dipped them in DI water, then in the RA > Hematoxylin 2 on the stainer, then in DI again, air-dried and > coverslipped them, and the blue dots were there. The only way we got > rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > > > > Thanks for your input, and if you can recommend a > different, reasonably priced hematoxylin, that would be awesome. > > > > Cheers, > > > > Sandy > > > > > > > > Sandra J. Cheasty, HT (ASCP) > > > > Histology & Necropsy Supervisor > > > > UW-Madison, School of Veterinary Medicine > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 22 Sep 2015 22:36:09 +0000 From: "Lewis, Patrick" To: " (Histonet at lists.utsouthwestern.edu)" Subject: [Histonet] Quick H202 quenching question. Message-ID: <3903BE18914F4440834F0E620415FFCA3CBA2228 at PPWEXD01d.childrens.sea.kids> Content-Type: text/plain; charset="us-ascii" If I have an IHC where I am staining 2 slides from the same block, one with one primary antibody and the other with a different primary antibody. And one antibody's slides have high nonspecific background, but the other antibody's slides have no background, Can I deduce that the background staining is not caused by insufficient H202 quenching/blocking? All the steps were the same, except for the primary antibody/secondary antibody. Also, they did have different epitope retrievals. But the wash buffers/blocking buffers/and substrate were the same. I am fairly confident that my background problems are related to this particular primary antibody, and not the actual quenching/blocknig from my IHC protocol, but I thought I'd check with you guys. Patrick. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 11 Date: Tue, 22 Sep 2015 16:59:55 -0600 From: "Gayle Callis" To: "'Manfre, Philip'" Cc: "Histonet" Subject: Re: [Histonet] Hematoxylin Precipitate and filtering Gill formulations Message-ID: <000301d0f58a$663f49a0$32bddce0$@bresnan.net> Content-Type: text/plain; charset="us-ascii" Yes, I have used Gill 1, 2 and 3 even in the early days of buying these formulations from a vendor, and always filtered them before using. Old school habits never changed.............. Gayle Callis -----Original Message----- From: Manfre, Philip via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 12:26 PM To: Elizabeth Chlipala ; Tim H Cc: 'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu) Subject: Re: [Histonet] Hematoxylin Precipitate Wow, I agree with Liz. There should not routinely be "so much tissue washing off". There is a fundamental problem, if this is the case. With regards to hematoxylin, have you tried Gill's Hematoxylin, 1, 2, or 3? These do not need filtering and do not produce a precipitate. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_manfre at merck.com ------------------------------ Message: 12 Date: Wed, 23 Sep 2015 07:55:59 -0400 From: "Manfre, Philip" To: Tim H , "gayle.callis at bresnan.net" , "'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)" Subject: Re: [Histonet] Hematoxylin Precipitate Message-ID: <558A4571351D0C42BD923F403F4198C40102EF9B26BF at USCTMXP51014.merck.com> Content-Type: text/plain; charset="us-ascii" Well Tim, In the 31 years I have been in histology, I obviously have done all lab work with my eyes closed, never looking at the equipment or solutions I have been using. I guess my stint in a high throughput contract lab taught me nothing nor did my 25 + years in big Pharma, notorious for hiring every hack who knows what a microscope slide is. You suggested that I sit around getting published... I am published, BTW, but that accounts for 0.01% of my experiences in the lab, yes, in the lab processing, embedding, sectioning (quite good at that), cryosectioning, performing and troubleshooting IHC and histochemical stains. Something I may have learned, and maybe you will when you catch up, is not assume you know what someone else's work experiences may have been or are. Histology occurs in many environments with differing equipment, reagents, processing styles, etc. Some are better than others. I guess our inadequate processing methods fall short, leaving far too much tissue on the slide where it belongs, and thus we don't have big tissue globs clogging everything, nor do we see precipitate with the hematoxylin used. Traditional formulations of hematoxylin, sure, absolutely filter. For anyone who benefits from filtering, why not continue. No harm will come. I was merely trying to advise someone who seemed to have an issue I thought I could help with. Now I realize all issues should be relegated to Tim, the Charles Churukian of our times. Look him up, if you're stumped by the reference. Maybe I'll head for Wacky Tabacky land for a vacation. It is quite beautiful there... Phil. -----Original Message----- From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 5:04 PM To: gayle.callis at bresnan.net; Histonet Subject: Re: [Histonet] Hematoxylin Precipitate Liz, Phillip and all that are interested, I take it you have guys never looked at or had someone else examine what is at the bottom of the Hematoxylin filter after you put through a day's work. There will be tissue particles of tissue along with other contaminates, I am not saying you are going to see a complete LEEP sitting at the bottom but you will have contamination. Liz, maybe the tissue super adheres up there in wacky tabacky country and Phillip sitting in a research facility (are you even involved with processing and staining of slides or just publishing articles?) but in the rest of the United States, I can guarantee you there will be some tissue in the Hematoxylin if you use a traditional dip and dunk system. Liz, you do bring up a good point about having tissue in every container. To a degree you will have tissue in every reagent container. I was assuming and maybe unjustly that most labs are using Good Laboratory Practice and discard their reagents after they have used them for a staining session. This topic was about Hematoxylin Precipitate and "small spore or pollen-like blue dots" which I did not say was tissue, I was passing along some of my 23 years of knowledge. Listen; don't take my word for it. You can have Ventana come out to your facility and filter all your reagents and stains and have them tell you what is in your solutions. Next time just pass along good information and criticize those trying to help! To many people like to make a negative on this site of people trying to truly help. This is why I rarely post on Histonet but instead directly email the person. By no means am I promoting Ventana/Roche products. I am just passing along information that might be helpful. Tim Disclaimer: This information is by no means is meant for any weak stomach individuals or those preparing for the zombie apocalypse. > From: gayle.callis at bresnan.net > To: thigginsht at msn.com; histonet at lists.utsouthwestern.edu > Subject: RE: [Histonet] Hematoxylin Precipitate > Date: Tue, 22 Sep 2015 12:27:21 -0600 > > Sandy, > > After years of using Richard Allan's hematoxylin 2 with great success, if > we didn't filter daily before use, we had stain precipitate on sections. > Some of this comes from the hematoxylin continuing to oxidize in open air, > bacteria and other "crud". Tim is absolutely correct ignoring > manufacturers no filtering instructions. Being old school, we were taught > to faithfully filter any hematoxylin, regardless of progressive or > regressive types. If we topped off hematoxylin 2 or used new stock, the > stain was filtered into a CLEAN staining container/dish. Keep an extra > container around if possible. We used a medium fast filter paper, Whatman > 54. I realize this takes time but junk on a slide is NOT good thing, > especially after IHC staining and have a photo to show this - the result of > being lazy and not filtering the hematoxylin on that particular day. > > We used a distilled water rinse before hematoxylin2, but DI H2O will be > contaminated with cellular debris and last hydrating alcohol carryover. > Change DI water frequently if you have many runs in a day. We used 1 > minute running tap water rinses after hematoxylin, clearant and bluing. If > you are using running water rinses, take a look at the blue ppt in the post > hematoxylin container as you don't want that sticking to sections. Non > running water rinses should be changed after each H&E run in my opinion. > Adequate clean water rinses are important to not have carry over of clearant > into bluing reagents or bluing reagent into eosin in order to maintain > correct pH for staining. > > Good luck > > Gayle M. Callis > HTL/HT/MT(ASCP) > > -----Original Message----- > From: Tim H via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, September 22, 2015 11:25 AM > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Hematoxylin Precipitate > > You should be filtering your Hematoxylin on a daily basis regardless of what > the manufactures says. We use to filter twice a day since we did a > traditional overnight run and then again in the afternoon for specimens that > had been microwave processed. So much tissue washes off in the solutions > they should be changed or filtered fairly regularly to try and prevent cross > contamination on the slides. > > You can also try increasing your rinse times and see if that doesn't help as > well. > > Thanks, > > Tim > > > > Message: 1 > > Date: Mon, 21 Sep 2015 15:14:39 -0500 > > From: "Sandra Cheasty" > > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > > > Subject: [Histonet] Hematoxylin Precipitate > > Message-ID: <4cda87133587e64c965ce6c356d18f59 at svm.vetmed.wisc.edu> > > Content-Type: text/plain; charset="us-ascii" > > > > Hello all, > > > > Has anyone using Richard Allen Hematoxylin-2 noticed an > odd artifact on the slides after using the Hematoxylin for more than a few > days on their stainer? We are seeing small spore or pollen-like blue dots > here and there on the slides. It is not coming from the water bath or our > water supply on the stainer. I used sterile gloves, opened a new case of > slides, dipped them in DI water, then in the RA Hematoxylin 2 on the > stainer, then in DI again, air-dried and coverslipped them, and the blue > dots were there. The only way we got rid of the blue artifact was to use new > RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > > > > Thanks for your input, and if you can recommend a > different, reasonably priced hematoxylin, that would be awesome. > > > > Cheers, > > > > Sandy > > > > > > > > Sandra J. Cheasty, HT (ASCP) > > > > Histology & Necropsy Supervisor > > > > UW-Madison, School of Veterinary Medicine > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 13 Date: Wed, 23 Sep 2015 15:33:30 +0000 From: Nancy Schmitt To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Cryostate decon ANP.23410 Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C360115A4B354 at PEITHA.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" Good Morning- Defrost and decontaminate with TB disinfectant weekly if used daily. How are you best managing this and what are you using to decontaminate for TB? Thank you Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 14 Date: Wed, 23 Sep 2015 16:04:46 +0000 From: Michael Ann Jones To: Nancy Schmitt , "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] Cryostate decon ANP.23410 Message-ID: Content-Type: text/plain; charset="us-ascii" We do maybe 11 frozens/month and so decon/defrost quarterly. Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com On 9/23/15, 9:33 AM, "Nancy Schmitt via Histonet" wrote: >Good Morning- >Defrost and decontaminate with TB disinfectant weekly if used daily. How >are you best managing this and what are you using to decontaminate for TB? >Thank you > >Nancy Schmitt MLT, HT(ASCP) >United Clinical Laboratories >Dubuque, IA 52001 > > > > > >NOTICE: This email may contain legally privileged information. The >information >is for the use of only the intended recipient(s) even if addressed >incorrectly. If you are not the intended recipient, please notify the >sender >that you have received it in error and then delete it along with any >attachments. Thank you. > > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 142, Issue 19 ***************************************** IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is strictly prohibited. Thank you for your cooperation. From bburnett at CapeCodHealth.org Wed Sep 23 13:34:02 2015 From: bburnett at CapeCodHealth.org (Burnett, Brandy) Date: Wed, 23 Sep 2015 18:34:02 +0000 Subject: [Histonet] Kappa/Lambda bone marrow Message-ID: Does anyone out there have a Kappa and Lambda protocol for ISH on the Ventana BenchMark Ultra? Was also wondering what type of decal should be used for ISH? Thanks! Brandy Burnett BS,HTL(ASCP) QIHC bburnett at capecodhealth.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk at CapeCodHealth.org From kiran_g at sbcglobal.net Wed Sep 23 13:43:58 2015 From: kiran_g at sbcglobal.net (Kiran) Date: Wed, 23 Sep 2015 11:43:58 -0700 Subject: [Histonet] RUO expiration In-Reply-To: References: Message-ID: <4FE25E0E-5FCB-4B78-A611-4EF05418213A@sbcglobal.net> Dear Experts, How do you guys determine expiration dates for RUO Abs? Thx Kiran > On Sep 23, 2015, at 10:53 AM, Chung, Fai via Histonet wrote: > > Ventana is selling their Discovery instrument for research use only. I am wondering if anyone know if a complete full validation is done on the instrument, can we use it on clinical cases as a "laboratory developed test". For antibody classified as RUO, we are able to use it on patient slide as a "laboratory developed" test as long as we do a complete full validation. > > Thanks. > Fai > > -----Original Message----- > From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] > Sent: Wednesday, September 23, 2015 10:00 AM > To: histonet at lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 142, Issue 19 > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Hematoxylin Precipitate (Tim H) > 2. Re: Hematoxylin Precipitate (Elizabeth Chlipala) > 3. Re: Hematoxylin Precipitate (Manfre, Philip) > 4. Re: Hematoxylin Precipitate (Gayle Callis) > 5. Webinar presentation hand-outs (Elaine allison Hoffman) > 6. Re: Webinar presentation hand-outs (Morken, Timothy) > 7. Re: Hematoxylin Precipitate (Tim H) > 8. Elastic Stain on the Benchmark Special Stainer (BMSS)? > (P Sicurello) > 9. Re: Hematoxylin Precipitate (Elizabeth Chlipala) > 10. Quick H202 quenching question. (Lewis, Patrick) > 11. Re: Hematoxylin Precipitate and filtering Gill formulations From Allison.Scott at harrishealth.org Wed Sep 23 17:30:11 2015 From: Allison.Scott at harrishealth.org (Scott, Allison D) Date: Wed, 23 Sep 2015 22:30:11 +0000 Subject: [Histonet] Fungus contamination on AFB slides Message-ID: Hello to all in histoland. We are currently having a problem with candida on our AFB slides. We are doing the stain by hand due to the unavailability of the AFB kits from ventana. We are using bottled sterile water for rinses and the slides are not being put in coplin jars for staining. All solutions are being pipetted on to the slides. All alcohols are fresh and the kit which is from American MasterTech is new, just opened. This by the way is a cytology case(cystospin). Containers to run down to water have been bleached. Any suggetions where to go next. Allison Scott LBJ Hospital Houston, TExas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From tony.henwood at health.nsw.gov.au Wed Sep 23 22:13:17 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 24 Sep 2015 03:13:17 +0000 Subject: [Histonet] Fungus contamination on AFB slides In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A715701976540C3@xmdb04.nch.kids> Hi Allison, You probably need to track down the source of the Candida. I would suggest you cytocentrifuge each solution, air-dry the cytospin slides and stain with a giemsa. Most fungi (esp candida) will show up. See: Henwood A (2013) "Fungal Contamination of Hanks Solution" Diagnostic Cytopathology 41(5):453-455 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Scott, Allison D via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 24 September 2015 8:30 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fungus contamination on AFB slides Hello to all in histoland. We are currently having a problem with candida on our AFB slides. We are doing the stain by hand due to the unavailability of the AFB kits from ventana. We are using bottled sterile water for rinses and the slides are not being put in coplin jars for staining. All solutions are being pipetted on to the slides. All alcohols are fresh and the kit which is from American MasterTech is new, just opened. This by the way is a cytology case(cystospin). Containers to run down to water have been bleached. Any suggetions where to go next. Allison Scott LBJ Hospital Houston, TExas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From LRaff at uropartners.com Thu Sep 24 10:23:52 2015 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 24 Sep 2015 15:23:52 +0000 Subject: [Histonet] CAP All Common Checklist for Anatomic Pathology Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B40FC40@COLOEXCH01.uropartners.local> Hello Histo-netters: Do any of you have procedures written that cover the section on Test Method Validation/Verification? We do verifications/validations of new antibodies but don't really have a specific written policy/procedure. If any of you have a written procedure and would like to share, our lab would appreciate it! Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 https://downsizemaybe.wordpress.com From litepath2000 at yahoo.com Thu Sep 24 11:14:24 2015 From: litepath2000 at yahoo.com (NYSHisto) Date: Thu, 24 Sep 2015 16:14:24 +0000 (UTC) Subject: [Histonet] veterinary IHC In-Reply-To: <1964723234.1183702.1442520254317.JavaMail.yahoo@mail.yahoo.com> References: <1964723234.1183702.1442520254317.JavaMail.yahoo@mail.yahoo.com> Message-ID: <1871188349.388416.1443111264721.JavaMail.yahoo@mail.yahoo.com> Thank you to all who responded From: NYSHisto via Histonet To: Histonet List Sent: Thursday, September 17, 2015 4:04 PM Subject: [Histonet] veterinary IHC Looking for academic facility/lab that runs IHC on canines samples.? Please contact me of list.Thanks in advance _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree at uwhealth.org Thu Sep 24 12:27:49 2015 From: LSebree at uwhealth.org (Sebree Linda A) Date: Thu, 24 Sep 2015 17:27:49 +0000 Subject: [Histonet] NY-ESO-1 immunohistochemical stain Message-ID: <77DD817201982748BC67D7960F2F76AF173957@UWHC-MBX13.uwhis.hosp.wisc.edu> Good afternoon, Wondering if there are any reference labs offering this stain to perform on FFPE human tissue. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From KSimeone at leavittmgt.com Thu Sep 24 12:43:08 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Thu, 24 Sep 2015 17:43:08 +0000 Subject: [Histonet] FULLTIME DAY SHIFT (first shift) AND FULL TIME NIGHT SHIFT (IHC) POSITION DELRAY BCH FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C4D2DDB61@vm-email.leavittmgt.com> Hi Histonetters! We are looking for TWO full time licensed histotechs here in our very busy Delray Beach, Florida dermatology laboratory. These are permanent full time, (40 hours) position with benefits (medical/401k/vacation). Annual salary in the $50k range (NIGHT SHIFT DIFF AVAILABLE). THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! DAYTIME POSTION: *full time position Mon-Fri 8a-5:30p (start time may vary) *MUST be licensed as a FLORIDA HISTOTECHNICIAN OR HISTOTECHNOLOGIST (NO pending licensure pls) *PREFER experience but WILL TRAIN a knowledgeable, willing candidate *duties to include (but not limited to): grossing, microtomy, accessioning, embedding and general histology *must be self motivated, reliable and a team player DAYTIME SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY: https://advancedderm.applicantpro.com/jobs/240090.html NIGHT POSITION: *Full time position Mon-Fri 10:30p-7:00a (IMMUNOHISTOCHEMISTRY/SPECIALS department) *MUST be licensed as a FLORIDA HISTOTECHNOLOGIST (this is NOT negotiable) *EXPERIENCE WITH IHC A MUST! Leica (BOND) and Roche/Ventana equipment experience preferred *Must be able to multi-task special stains *MUST have at LEAST 2 years experience. Please DO NOT respond if you do not possess EXPERIENCE in this area! *must be confidant, quick learner, self motivated, reliable and a team player NIGHT SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY: https://advancedderm.applicantpro.com/jobs/278020.html Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From tbraud at holyredeemer.com Thu Sep 24 13:18:16 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 24 Sep 2015 18:18:16 +0000 Subject: [Histonet] BMSS Elastic Message-ID: <48E053DDF6CE074DB6A7414BA05403F803DFEE@HRHEX02-HOS.holyredeemer.local> Just a note: The results of the stain are supposed to be elastic fibers: Blue-black to black. If you stain something with a bluish tint, then counterstain over it with Van Gieson's (red)... Well, red and blue make purple. Even the picture that accompanies the kit in the catalogue shows the fibers in purple. Since all Ventana instruments work on the principle of 4 minute intervals, you can increase the level of staining in the Elastic Stain, 4 minutes at a time up to 32 minutes. I think the decolorization step and Van Gieson's are set to the 4 minute minumum, but check to see. The trick being to increase the Elastic Stain and decrease the differentiation (destaining) step, or the counterstain (Van Gieson's step) bearing in mind that the Van Gieson's will slightly continue to decolorize the elastic. Best of luck. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 ************************************* From HornHV at archildrens.org Thu Sep 24 13:19:22 2015 From: HornHV at archildrens.org (Horn, Hazel V) Date: Thu, 24 Sep 2015 13:19:22 -0500 Subject: [Histonet] FW: CAP All Common Checklist for Anatomic Pathology In-Reply-To: <6347C6D2B080534F9B5C2B08436DCFAF0B40FC40@COLOEXCH01.uropartners.local> References: <6347C6D2B080534F9B5C2B08436DCFAF0B40FC40@COLOEXCH01.uropartners.local> Message-ID: <25A4DE08332B19499904459F00AAACB71A39C4FCC8@EVS1.archildrens.org> Dr. Raff, If the links do not work, please let me know. This is our procedure and our validation form. http://ppm1.archildrens.org/dotNet/documents/?docid=13171&mode=view http://ppm1.archildrens.org/dotNet/documents/?docid=14275&mode=view Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org -----Original Message----- From: Lester Raff MD via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 24, 2015 10:24 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CAP All Common Checklist for Anatomic Pathology Hello Histo-netters: Do any of you have procedures written that cover the section on Test Method Validation/Verification? We do verifications/validations of new antibodies but don't really have a specific written policy/procedure. If any of you have a written procedure and would like to share, our lab would appreciate it! Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 https://downsizemaybe.wordpress.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madary at verizon.net Thu Sep 24 15:39:47 2015 From: madary at verizon.net (madary at verizon.net) Date: Thu, 24 Sep 2015 15:39:47 -0500 (CDT) Subject: [Histonet] tissue microarray system suggestions Message-ID: <28901570.435827.1443127187207.JavaMail.root@vms170015.mailsrvcs.net> From mburns at atlanticurologyclinics.com Fri Sep 25 10:23:12 2015 From: mburns at atlanticurologyclinics.com (Melissa Burns) Date: Fri, 25 Sep 2015 15:23:12 +0000 Subject: [Histonet] Dye ? Message-ID: <8F26C28A6B397C47BFC334F7B2D6FD9880ACC790@AUC-Exchange1.gsuro.com> Hello All- I'm having an ongoing issue that I'm hoping all of you smart people can help me with! We are talking about prostate needle biopsy specimens. We have one company that does a genetic test that is insisting that there is a dye present in the tissue we are sending. None of the other genetics companies we send to have had an issue....just this one....and not on every case we send them! Imagine my confuse :) The specimens are sent in 10% NBF. There is no dye used in grossing or processing. Am I missing something? Somewhere that dye may be sneaking in? We are to the point now that they want to come to the lab and the collecting surgery centers and see if they can figure it out..... Baffled.... Melissa From igor.deyneko at gmail.com Fri Sep 25 10:45:35 2015 From: igor.deyneko at gmail.com (Igor Deyneko) Date: Fri, 25 Sep 2015 11:45:35 -0400 Subject: [Histonet] Contamination & Floaters in sections Message-ID: Dear Histonetters, I wanted to get your input in reconciling and reporting contamination and floaters (C &F) in your slides. Do you even track such instances, of you do, do you differentiate between C&F, what are the metrics that you use and what do you do with that data? I have implemented a very basic tracking system, I get the slides and enter the information on who embedded and sectioned the slide and also the clinician who has performed the procedure per Quality Control department?s orders. Any input regarding the usage of data and what to look for to reduce such instances would be appreciated. Thank you in advance, *Igor Deyneko* Quality Control & Compliance Manager NYU Langone Medical Center Department of Anatomic Pathology 560 First Avenue, New York, NY 10016 From mjones at metropath.com Fri Sep 25 10:53:15 2015 From: mjones at metropath.com (Michael Ann Jones) Date: Fri, 25 Sep 2015 15:53:15 +0000 Subject: [Histonet] Dye ? Message-ID: Is it possible that the clinician is using some sort of dye during collection? Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com On 9/25/15, 9:23 AM, "Melissa Burns via Histonet" wrote: >Hello All- > >I'm having an ongoing issue that I'm hoping all of you smart people can >help me with! We are talking about prostate needle biopsy specimens. > >We have one company that does a genetic test that is insisting that there >is a dye present in the tissue we are sending. None of the other genetics >companies we send to have had an issue....just this one....and not on >every case we send them! Imagine my confuse :) > >The specimens are sent in 10% NBF. There is no dye used in grossing or >processing. > >Am I missing something? Somewhere that dye may be sneaking in? > >We are to the point now that they want to come to the lab and the >collecting surgery centers and see if they can figure it out..... > >Baffled.... > >Melissa > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From foreightl at gmail.com Fri Sep 25 11:02:31 2015 From: foreightl at gmail.com (Patrick Laurie) Date: Fri, 25 Sep 2015 12:02:31 -0400 Subject: [Histonet] Dye ? In-Reply-To: <8F26C28A6B397C47BFC334F7B2D6FD9880ACC790@AUC-Exchange1.gsuro.com> References: <8F26C28A6B397C47BFC334F7B2D6FD9880ACC790@AUC-Exchange1.gsuro.com> Message-ID: Do you perhaps use eosin on your processors to help visualize the biopsies for embedding? It can fluoresce. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Fri, Sep 25, 2015 at 11:23 AM, Melissa Burns via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello All- > > I'm having an ongoing issue that I'm hoping all of you smart people can > help me with! We are talking about prostate needle biopsy specimens. > > We have one company that does a genetic test that is insisting that there > is a dye present in the tissue we are sending. None of the other genetics > companies we send to have had an issue....just this one....and not on every > case we send them! Imagine my confuse :) > > The specimens are sent in 10% NBF. There is no dye used in grossing or > processing. > > Am I missing something? Somewhere that dye may be sneaking in? > > We are to the point now that they want to come to the lab and the > collecting surgery centers and see if they can figure it out..... > > Baffled.... > > Melissa > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From elaineahoffman55 at yahoo.com Fri Sep 25 12:18:32 2015 From: elaineahoffman55 at yahoo.com (Elaine allison Hoffman) Date: Fri, 25 Sep 2015 17:18:32 +0000 (UTC) Subject: [Histonet] Microscope Maintenance Message-ID: <2066071169.1033834.1443201512343.JavaMail.yahoo@mail.yahoo.com> Hello Histonetters... I'm putting together a microscope maintenance procedure and need some help.? Does anyone have a procedure that they would be willing to share?? Nothing major, just the basic routine maintenance that would be done to the microscope on a daily, weekly, and monthly basis.? Most procedures listed on the internet, they are like major endeavors of taking the microscope apart and putting it back together again.? We like to leave that business to the professionals who do our PM's once a year.? Any suggestions or help would be greatly appreciated!? Thanks! Elaine Hoffman The Gastroenterology Clinic & Endoscopy Center, IncGI Pathology From tbraud at holyredeemer.com Fri Sep 25 12:37:39 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 25 Sep 2015 17:37:39 +0000 Subject: [Histonet] mystery dye Message-ID: <48E053DDF6CE074DB6A7414BA05403F803E313@HRHEX02-HOS.holyredeemer.local> Dear Baffled - Could there possible contamination from other specimens that are being processed at the same time? For example, skins or other tissues with inked margins? Just thinking out loud. Pondering, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 6. Dye ? (Melissa Burns) ------------------------------ Message: 6 Date: Fri, 25 Sep 2015 15:23:12 +0000 From: Melissa Burns Subject: [Histonet] Dye ? Hello All- I'm having an ongoing issue that I'm hoping all of you smart people can help me with! We are talking about prostate needle biopsy specimens. We have one company that does a genetic test that is insisting that there is a dye present in the tissue we are sending. None of the other genetics companies we send to have had an issue....just this one....and not on every case we send them! Imagine my confuse :) The specimens are sent in 10% NBF. There is no dye used in grossing or processing. Am I missing something? Somewhere that dye may be sneaking in? We are to the point now that they want to come to the lab and the collecting surgery centers and see if they can figure it out..... Baffled.... Melissa ************************************** From avistarop at ffyb.uba.ar Mon Sep 28 12:41:26 2015 From: avistarop at ffyb.uba.ar (avistarop at ffyb.uba.ar) Date: Mon, 28 Sep 2015 14:41:26 -0300 (ART) Subject: [Histonet] IHC: Cytokeratin 7 and CD68 antibodies Message-ID: <354123dec67e5bf51acd490290004d91.squirrel@huemul.ffyb.uba.ar> Hello to everyone! Has anyone used -anti-Cytokeratin 7 (SP52) Rabbit Monoclonal Primary Antibody (VENTANA) - anti-CD68 (KP-1) Primary Antibody (VENTANA) both on FFPE tissue? Could I please get a copy of your protocols? Thank you so much. Aldana Vistarop Laboratorio Biolog?a Molecular, Servicio de Anatom?a Patol?gica, Hospital de Ni?os Ricardo Guti?rrez. Gallo 1330 C1425EFD Ciudad de Buenos Aires Argentina TE: (5411) 4962-9138. FAX: (5411)4962-9138. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu From kenneth.metzger at aruplab.com Tue Sep 29 11:10:48 2015 From: kenneth.metzger at aruplab.com (Metzger, Kenneth) Date: Tue, 29 Sep 2015 16:10:48 +0000 Subject: [Histonet] Staples and Sutures Message-ID: <3855827CD3E36249A30D57F6F896F8F10229ADC336@EXMBX2.aruplab.net> Hello All, Recently we have had a marked increase in staples and sutures in our tissue that arrives for processing. I have tried to relay to our pathologists and PA's the difficulty in cutting sections and the tissue destruction in getting staples and sutures out once the tissue is processed to paraffin. I was told by some of them that in their experience histology should dealing with this issue. Though my own opinion is this is position is not valid, I've been asked to collect opinions from other histology departments if there are protocols that the grossing individual should be removing the foreign object. Please give me any feedback...Thanks Ken Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metzger at aruplab.com ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From jmcgough at clinlab.com Tue Sep 29 11:36:42 2015 From: jmcgough at clinlab.com (=?utf-8?Q?Jason_McGough?=) Date: Tue, 29 Sep 2015 10:36:42 -0600 Subject: [Histonet] Cassette Printers In-Reply-To: <3855827CD3E36249A30D57F6F896F8F10229ADC336@EXMBX2.aruplab.net> References: <3855827CD3E36249A30D57F6F896F8F10229ADC336@EXMBX2.aruplab.net> Message-ID: We have been using a Leica IP-C cassette printer for the past 8 years and have experienced some issues this past year with it. Anybody know what the life expectancy is of this cassette printer? What other cassette printers work well? Thank you in advance for your response. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgough at clinlab.com www.clinlab.com From relia1 at earthlink.net Tue Sep 29 11:48:36 2015 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 29 Sep 2015 12:48:36 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin 9-29-2015 Are you enjoying the Fall season? Message-ID: <018001d0fad6$b1dcb040$159610c0$@earthlink.net> Hi Histopeeps! How are you? Are you enjoying the sights, sounds and flavors of Fall? I know I am enjoying college football after all I am a southern gal and you know how we southerners are about our Football Saturdays in the South. I am still patiently waiting for "The Real Fall stuff". You know like crisp cool air, beautiful fall colors, crunching leaves and steaming bowls of soup. Because it is still 90 degrees here in Orlando!!!! What are you enjoying most of all about this season? I also wanted to tell you about some new job opportunities. These are some of my best clients and they are ready to interview and hire right away. HOT HISTOLOGY OPENINGS: Histotech - Fayetteville, AR Histotech - Nashville, TN Histotech - Reno, NV CLIA qual to gross and NV lic req. Histotechnician - Atlanta, GA Histotechnician - Longview, TX (Learn MOHS!!) Lead Histotech - Atlanta, GA Histotech - Austin, TX Histotech - Atlanta, GA Histotech - East of Chicago - Hammond, IN If you or anyone you know might be interested in any of these opportunities or would like help with a job search in another area of the USA please contact me. I can be reached at relia1 at earthlink.net or toll free at 866-607-3542. Remember if I place someone you refer to me you will earn a referral bonus!! Have a great week!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Michael.Baker at cchmc.org Tue Sep 29 12:12:38 2015 From: Michael.Baker at cchmc.org (Baker, Michael) Date: Tue, 29 Sep 2015 17:12:38 +0000 Subject: [Histonet] Staples and Sutures In-Reply-To: References: Message-ID: Ken, the individual grossing should remove all the staples and sutures they can. If nothing else, it?s just good manners. Michael. > Hello All, > > Recently we have had a marked increase in staples and sutures in our tissue that arrives for processing. I have tried to relay to our pathologists and PA's the difficulty in cutting sections and the tissue destruction in getting staples and sutures out once the tissue is processed to paraffin. I was told by some of them that in their experience histology should dealing with this issue. Though my own opinion is this is position is not valid, I've been asked to collect opinions from other histology departments if there are protocols that the grossing individual should be removing the foreign object. Please give me any feedback...Thanks > > Ken ---- Michael Baker, M.D. CCHMC Pathology T (513) 636-4261 F (513) 636-3924 From adhall at ael.com Tue Sep 29 12:17:03 2015 From: adhall at ael.com (Angela Hall) Date: Tue, 29 Sep 2015 12:17:03 -0500 Subject: [Histonet] Staples and Sutures In-Reply-To: <3855827CD3E36249A30D57F6F896F8F10229ADC336@EXMBX2.aruplab.net> References: <3855827CD3E36249A30D57F6F896F8F10229ADC336@EXMBX2.aruplab.net> Message-ID: Ken, I'm, currently, checking our SOPs with updates from CAP. The one that stuck out to me under "Quality Management", see below: **NEW** 07/28/2015 ANP.10038 Anatomic Pathology Checklist 07.28.2015 Tissue Sample Quality Phase II There is a procedure that describes the process by which histotechnologists provide feedback to submitting pathologists and pathology assistants on the quality of the gross tissue sections received for tissue processing. NOTE: Inadequate fixation, overly thick tissue sections, non- decalcified bone, the presence of staples, etc., can lead to poor quality histologic sections and/or poor quality special stains/special studies. This requirement applies to both laboratories that gross tissue and perform all processing onsite, as well as laboratories that gross tissue and send it to another laboratory for processing, embedding, and sectioning (regardless of the outside laboratory's accrediting organization). Records of such feedback and corrective action taken when problems are identified may be incorporated into the laboratory's quality management program. Angela D. Hall, BA, HT(ASCP)CM Histology Department American Esoteric Laboratories www.ael-east.com ________________________________ Tel +423 586 3240 ext 1019 or 1041 Fax +423 714 2001 ? This message and any files transmitted with it may contain privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message, you must not disseminate, copy or take any action in reliance on it. If you have received this message in error, please notify?the?sender ?immediately. -----Original Message----- From: Metzger, Kenneth [mailto:kenneth.metzger at aruplab.com] Sent: Tuesday, September 29, 2015 12:11 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Staples and Sutures Hello All, Recently we have had a marked increase in staples and sutures in our tissue that arrives for processing. I have tried to relay to our pathologists and PA's the difficulty in cutting sections and the tissue destruction in getting staples and sutures out once the tissue is processed to paraffin. I was told by some of them that in their experience histology should dealing with this issue. Though my own opinion is this is position is not valid, I've been asked to collect opinions from other histology departments if there are protocols that the grossing individual should be removing the foreign object. Please give me any feedback...Thanks Ken Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metzger at aruplab.com ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From CDavis at che-east.org Tue Sep 29 12:38:57 2015 From: CDavis at che-east.org (Davis, Cassie) Date: Tue, 29 Sep 2015 13:38:57 -0400 Subject: [Histonet] increase in staples and sutures In-Reply-To: References: Message-ID: RE: increase in staples and sutures Ken, this really depends on the amount of support you get from your Medical Director. Any staple or sutures are removed at grossing by the PA or embedding or microtomy by the histology technician still affects the tissue. Either way the pathologist hasn't typically seen the tissue or slide yet. This is a fight that seems to go on everywhere that does larger specimens. We record it at microtomy as a quality issue of grossing if there is a staple or suture in the block just for documentation purposes. The only way there can be can justification for the tech removing it is the PA time and blade that get ruined cost more than the HT that is embedding or doing microtomy. I'm not sure why it is not taken into consideration the PA's time and blade is already on the specimen in order to gross it so why should both budgets suffer. I wish you well on this fight, please share if you make good progress. Cassie Davis Message: 2 Date: Tue, 29 Sep 2015 16:10:48 +0000 From: "Metzger, Kenneth" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Staples and Sutures Message-ID: <3855827CD3E36249A30D57F6F896F8F10229ADC336 at EXMBX2.aruplab.net> Content-Type: text/plain; charset="us-ascii" Hello All, Recently we have had a marked increase in staples and sutures in our tissue that arrives for processing. I have tried to relay to our pathologists and PA's the difficulty in cutting sections and the tissue destruction in getting staples and sutures out once the tissue is processed to paraffin. I was told by some of them that in their experience histology should dealing with this issue. Though my own opinion is this is position is not valid, I've been asked to collect opinions from other histology departments if there are protocols that the grossing individual should be removing the foreign object. Please give me any feedback...Thanks Ken Kenneth G Metzger HTL(ASCP) Histology Supervisor ARUP Labs Salt Lake City, Utah Phone: (801)583-2787 ext. 3101 Fax: (801) 584-5244 Email: kenneth.metzger at aruplab.com Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From patrick.lewis at seattlechildrens.org Tue Sep 29 13:29:54 2015 From: patrick.lewis at seattlechildrens.org (Lewis, Patrick) Date: Tue, 29 Sep 2015 18:29:54 +0000 Subject: [Histonet] Problem with ccmount drying out and reexposing tissue sections Message-ID: <3903BE18914F4440834F0E620415FFCA3CBAA191@PPWEXD01d.childrens.sea.kids> Hi everyone, Has anyone experienced a problem with sigmas cc/mount solution. I liberally cover my section with it to form a protective seal., But with my current bottle it seems that when it dries it re-exposes the tissue and does not form a protective layer. I end up having to reapply it. The 2nd apply seems to get me the protective layer that I want, but I am concerned that drying/exposure from the first application will damage the staining that I have. I don't want my post staining slides to be exposed to air for a length of time between hematoxylin and cover slipping. This is a fairly recent problem and I am wondering if I just have a bad batch of cc/mount. CAT# C9368-30 ML Lot# MKBR27838V I called Sigma and they are sending me a new bottle from a different lot. But they haven't experienced this problem before with cc/mount. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mills at 3scan.com Tue Sep 29 13:30:03 2015 From: mills at 3scan.com (Caroline Miller) Date: Tue, 29 Sep 2015 11:30:03 -0700 Subject: [Histonet] Tissue processor advice for a small start-up Message-ID: Hi All, Our company has reached the size where we can justify buying our own processor! Yippee!! However that also leads me to the issue of which one to buy :) We will have very small batches of blocks to process, approx 10 per process maybe twice per week, but we need the machine to be very responsive to different processes - we have a microscope that sections and images at the same time, therefore we do the staining prior to processing, and have to tweak the protocols to keep enough contrast int the tissue by the end. In short: - low volume - easy to use and change protocols - good support for servicing and fixing should things go wrong. - No formalin will touch the machine, and we will want to change out solvents from the standard alcohols and xylenes (therefore I need a machine who's warranty would not be affected by this. - I would love to get this all in one package and buy the service contract at the same time as the machine, preferably with the same company. - If it helps with the cost, then I also want to buy a paraffin microtome :) We are in San Francisco. Commercial replies are totally acceptable here, but please do not reply all. Thanks, as ever, histonet! yours, mills -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From dunatrsd at sbcglobal.net Tue Sep 29 13:38:27 2015 From: dunatrsd at sbcglobal.net (dusko trajkovic) Date: Tue, 29 Sep 2015 18:38:27 +0000 (UTC) Subject: [Histonet] Job Opportunity in So California Message-ID: <382613621.2897833.1443551907172.JavaMail.yahoo@mail.yahoo.com> Hallo Histonetters, We have a great job opportunity at Pfizer La Jolla site, inthe Investigative Pathology Lab. Here is a short list of qualifications desired. Emphasis onIF/IHC multiplexing. ?If you can fulfilthese, please contact me via email (dunatrsd at sbcglobal.net) and I will provide you with information on how to apply through the properchannels. This Pfizer site is nestled in one of the most beautifulplaces in Southern California, close to Torrey Pines and UCSD. Development and conduct of advancedImmunohistochemistry/Immunofluorescence procedures on preclinical study samplesin support of the rapidly expanding field of immuno-oncology.? Role toserve as a technical expert for the discipline and for projects supported. ? Responsibilities: ? Technical support of current Immunohistochemistry/Immunofluorescence workloadwith primary focus on immuno-oncology projects. ? Identification of Immunohistochemistry/Immunofluorescence assay/analysisgaps, development of work solutions ? Single plex and multi-plex ?Immunohistochemistry/Immunofluorescencemethod development/optimization/validation ? Technical expert for discipline and appropriate project teams/research unitworking groups ? Immunohistochemistry/Immunofluorescence process (simple/complex) mentor forjunior colleagues ? Development Expectation: ? Image Analysis (chromogenic or fluorescence) ? including data interpretation,integration and presentation ? More in depth project team involvement ? Drug Safety Team Lead ?Dusko Trajkovic Scientist-Investigative path Lab Pfizer Inc, La Jolla From Fawn.Bomar at HalifaxRegional.com Tue Sep 29 13:48:36 2015 From: Fawn.Bomar at HalifaxRegional.com (Fawn Bomar) Date: Tue, 29 Sep 2015 18:48:36 +0000 Subject: [Histonet] Morgue Refridgerator Temperature Message-ID: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA0A1619@EXCH-2K10.hrhs.com> Does anyone have a procedure for recording their morgue refridgerator temperatures that they would be willing to share? We do not perform autopsies at our facility any more but we have the refridgerator that the bodies are temporarily stored until they are picked up. We are monitoring the temperature but do not have an actual procedure written. I was wondering about a few key points such as, 1. What is the temperature range 2. Do you have a specific time allocated to check the temperature 3. If a body is placed in the fridge, do you give it a certain amount of time to get the temperature back into range 4. Do you have the other departments fill out a log of some sort to know if and when they place bodies in the fridge to account for any temperature changes Thank you Fawn Bomar ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From Timothy.Morken at ucsf.edu Tue Sep 29 15:07:26 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 29 Sep 2015 20:07:26 +0000 Subject: [Histonet] Dako computer? Message-ID: <761E2B5697F795489C8710BCC72141FF60317C2D@ex07.net.ucsf.edu> Does anyone have a left-over computer for the Dako Autostainer Plus you want to sell? Ours died and the service vendor is having a hard time finding a replacement. Any help is appreciated! Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From fpearsa at clemson.edu Tue Sep 29 16:10:19 2015 From: fpearsa at clemson.edu (Fran Pearsall) Date: Tue, 29 Sep 2015 17:10:19 -0400 Subject: [Histonet] Processors and Coverslippers Message-ID: <560AFE3B.8000203@clemson.edu> Hello every one, We are looking to purchase new equipment for our Histo lab. We are interested in the Thermo Gemini AS heated stainer and also the Thermo ClearVue Coverslipper. Can any one share their thoughts and opinions on these pieces of equipment? How well do the 2 work together or go with a stand alone coverslipper? Thank you for any suggestions. Fran Pearsall From esulkosky at gmail.com Tue Sep 29 19:04:37 2015 From: esulkosky at gmail.com (Eric Sulkosky) Date: Tue, 29 Sep 2015 20:04:37 -0400 Subject: [Histonet] Histotechnician opening in Bourbonnais, IL Message-ID: New Histology lab opening soon in Bourbonnais, Illinois. They are recruiting for a fulltime histotechnician to accession, gross, process, embed, cut and stain GI biopsies. ESSENTIAL JOB FUNCTIONS: Knowledge: Demonstrate proficiency with accessioning, grossing, microtomes, tissue processors, stainers, and equipment used to produce a stained and labeled slide Perform microscopic evaluation of own work and uses the information gained to reduce error and improve performance. Can recognize positive results. Can differentiate incorrectly performed work and correct it. Reviews all procedure manuals, safety manuals, equipment operator's manuals and current policy Demonstrate complete understanding of all test procedures. Possess leadership and interpersonal skills commensurate with assigned responsibilities. Learns specialty areas of test performance. Physical and Cognitive Requirements: Ability to master delicate mechanical movements carefully and safely perform tasks requiring incremental adjustments. Ability to physically, audibly, and visually (including color discrimination) perform all functions; with or without reasonable accommodation. Education: A.S. Degree in Histology required, B.S. Degree desired. Experience: H.T. and/or H.T.L. (ASCP), or equivalent experience and training required. Previous experience as a technician or technologist is preferred. Two years of experience at an acute care hospital of more than 200 beds, or a reference lab is desired. Interested candidates should forward resumes to: toconnorddc at gmail.com Please NO phone calls. From bcooper at chla.usc.edu Tue Sep 29 19:07:22 2015 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 30 Sep 2015 00:07:22 +0000 Subject: [Histonet] PFIC Case Material Message-ID: Good evening Histonet, Would any of you have access to, and be willing to share either blocks, or unstained slides from patients known to have progressive familial intrahepatic cholestasis (PFIC2 or PFIC3)? We're interested in working up a few antibodies, yet a sufficient number of positive cases is proving to be elusive. I'm willing to trade for control blocks that may have in our library. Please contact me if you can help. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper at chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From rsrichmond at gmail.com Tue Sep 29 19:47:45 2015 From: rsrichmond at gmail.com (Bob Richmond) Date: Tue, 29 Sep 2015 20:47:45 -0400 Subject: [Histonet] Staples and Sutures Message-ID: Kenneth G Metzger HTL(ASCP), Histology Supervisor, ARUP Labs, Salt Lake City, Utah asks: >>Recently we have had a marked increase in staples and sutures in our tissue that arrives for processing. I have tried to relay to our pathologists and PA's the difficulty in cutting sections and the tissue destruction in getting staples and sutures out once the tissue is processed to paraffin. I was told by some of them that in their experience histology should dealing with this issue. Though my own opinion is this is position is not valid, I've been asked to collect opinions from other histology departments if there are protocols that the grossing individual should be removing the foreign object. Please give me any feedback...Thanks<< What la-la land are your pathologists and PAs off in? I've caught plenty of hell in my life in pathology since 1964 - particularly back before the days of disposable blades - for not getting staples out of tissue before submitting it. This is definitely the responsibility of whoever's doing the grossing. Bob Richmond Samurai Pathologist Maryville TN From DKBoyd at chs.net Wed Sep 30 06:34:47 2015 From: DKBoyd at chs.net (Boyd, Debbie M) Date: Wed, 30 Sep 2015 11:34:47 +0000 Subject: [Histonet] [EXTERNAL] Re: Staples and Sutures In-Reply-To: References: Message-ID: <7EAFE982E328304DA6CE2B677BB76246AB496B21@TN001WEXMBX014.US.chs.net> Definitely the paths/PA's responsibility. Putting it in the histotech's court is like saying the car is responsible for hitting the pedestrian (not the driver). Crazy. We sometimes can see them when embedding but by then the tissue is not as pliable for easy removal without disturbing the tissue architecture. Margins are destroyed by the time we get the suture out. Ninety-nine percent of the time you can not find the knot to cut for removal. Staples can be embedded so deeply that they don't "shine" in reflective light. Only the blade finds them. If there is a whole line of them, the tissue is practically destroyed by pulling them out. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Bob Richmond via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 29, 2015 8:47 PM To: Histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Staples and Sutures Kenneth G Metzger HTL(ASCP), Histology Supervisor, ARUP Labs, Salt Lake City, Utah asks: >>Recently we have had a marked increase in staples and sutures in our tissue that arrives for processing. I have tried to relay to our pathologists and PA's the difficulty in cutting sections and the tissue destruction in getting staples and sutures out once the tissue is processed to paraffin. I was told by some of them that in their experience histology should dealing with this issue. Though my own opinion is this is position is not valid, I've been asked to collect opinions from other histology departments if there are protocols that the grossing individual should be removing the foreign object. Please give me any feedback...Thanks<< What la-la land are your pathologists and PAs off in? I've caught plenty of hell in my life in pathology since 1964 - particularly back before the days of disposable blades - for not getting staples out of tissue before submitting it. This is definitely the responsibility of whoever's doing the grossing. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From sbaldwin at mhhcc.org Wed Sep 30 08:25:45 2015 From: sbaldwin at mhhcc.org (Baldwin, Kathy) Date: Wed, 30 Sep 2015 13:25:45 +0000 Subject: [Histonet] TEMP POSITION FOR HISTOLOGIST Message-ID: <6764911d028f4c7288e62fd96542705c@exch02.mhhcc.org> Hi Histonetters Are there any of you traveling histologists. We need a position filled quickly (had one accepted then she changed her mind) starting October 5th for 3 possibly to 6 weeks. Have your company give me a call. Thanks S. Kathy Baldwin Histology/Cytology Supervisor Memorial Hospital and Health Care Center 800 West 9th St. Jasper, Indiana 47546 Office 812-996-0210 Fax 812-996-0232 Cell 812-887-3357 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From brendal.finlay at medicalcenterclinic.com Wed Sep 30 09:38:25 2015 From: brendal.finlay at medicalcenterclinic.com (Brendal Finlay) Date: Wed, 30 Sep 2015 09:38:25 -0500 Subject: [Histonet] Histology Position Open Message-ID: <001501d0fb8d$a9e56cd0$fdb04670$@finlay@medicalcenterclinic.com> We have a full time histology technologist position open in NW Florida. More information on the job and applying can be found at the link below. http://www.careerbuilder.com/jobseeker/jobs/jobdetails.aspx?Job_DID=JHN3926M 50NHDMLGM3H&siteid=cb_emailjob_us&showNewJDP=yes&IPATH=JEECXP&emailversion=c b_ej_a&utm_source=email-a-job&utm_medium=email&utm_campaign=regular-email-a- job&utm_term=2015-09-30&utm_content=job_detail_link Brendal Finlay, HT (ASCP) From brendal.finlay at medicalcenterclinic.com Wed Sep 30 10:46:59 2015 From: brendal.finlay at medicalcenterclinic.com (Brendal Finlay) Date: Wed, 30 Sep 2015 10:46:59 -0500 Subject: [Histonet] Histology Position Open In-Reply-To: <001501d0fb8d$a9e56cd0$fdb04670$@finlay@medicalcenterclinic.com> References: <001501d0fb8d$a9e56cd0$fdb04670$@finlay@medicalcenterclinic.com> Message-ID: <001a01d0fb97$3e4d2120$bae76360$@finlay@medicalcenterclinic.com> Sorry, I should have tiny url'd this link! Hopefully, this one works. http://tinyurl.com/oz266xh -----Original Message----- From: Brendal Finlay via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, September 30, 2015 9:38 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Histology Position Open We have a full time histology technologist position open in NW Florida. More information on the job and applying can be found at the link below. http://www.careerbuilder.com/jobseeker/jobs/jobdetails.aspx?Job_DID=JHN3926M 50NHDMLGM3H&siteid=cb_emailjob_us&showNewJDP=yes&IPATH=JEECXP&emailversion=c b_ej_a&utm_source=email-a-job&utm_medium=email&utm_campaign=regular-email-a- job&utm_term=2015-09-30&utm_content=job_detail_link Brendal Finlay, HT (ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brendal.finlay at medicalcenterclinic.com Wed Sep 30 11:10:39 2015 From: brendal.finlay at medicalcenterclinic.com (Brendal Finlay) Date: Wed, 30 Sep 2015 11:10:39 -0500 Subject: [Histonet] Histology Position in NW FL Message-ID: <000001d0fb9a$8c8842e0$a598c8a0$@finlay@medicalcenterclinic.com> Ok, apparently job links on Career Builder expire. If interested, please visit http://www.medicalcenterclinic.com/careers.asp and select employment opportunities to the right. You'll be taken to the Career Builder website page that lists the jobs for the Medical Center Clinic. The job listing for a Histology Technologist is posted with other opportunities that are available. I apologize for the constant emails. Thank you! Brendal From yesyes at comcast.net Wed Sep 30 11:13:48 2015 From: yesyes at comcast.net (yesyes at comcast.net) Date: Wed, 30 Sep 2015 16:13:48 +0000 (UTC) Subject: [Histonet] Crystal Analysis Message-ID: <1749801868.17281075.1443629628343.JavaMail.zimbra@comcast.net> From tawnia at ampianstaffing.com Wed Sep 30 12:06:26 2015 From: tawnia at ampianstaffing.com (Tawnia Lindsay) Date: Wed, 30 Sep 2015 17:06:26 +0000 Subject: [Histonet] job opportunities Message-ID: Hi Histoneters!!! I have a couple of fast start travel opportunities available. Both are in amazing locations with labs I have worked with in the past. One is looking for someone to start on Monday for 13 weeks in the Southwest and the other is on the 8th for 3 weeks in the Pacific Northwest. Please call me if you are interested!!! Tawnia Lindsay Senior Recruiter Ampian Staffing, Inc. 126 W Sego Lily Suite 110 Sandy UT 84070 O:877-229-6996 ext 2009 F:801-253-6127 email: tawnia at ampianstaffing.com Website: ampainstaffing.com From KSimeone at leavittmgt.com Wed Sep 30 12:24:44 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Wed, 30 Sep 2015 17:24:44 +0000 Subject: [Histonet] FULLTIME DAY SHIFT (first shift) AND FULL TIME NIGHT SHIFT (IHC) POSITION DELRAY BCH FL In-Reply-To: <43944B1DBAAC2846B7B9D626B5F1233C4D2DDB61@vm-email.leavittmgt.com> References: <43944B1DBAAC2846B7B9D626B5F1233C4D2DDB61@vm-email.leavittmgt.com> Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C4D2DDDED@vm-email.leavittmgt.com> Hi Histonetters! We are looking for TWO full time licensed histotechs here in our very busy Delray Beach, Florida dermatology laboratory. These are permanent full time, (40 hours) position with benefits (medical/401k/vacation). Annual salary in the $50k range (NIGHT SHIFT DIFF AVAILABLE). THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! DAYTIME POSTION: *full time position Mon-Fri 8a-5:30p (start time may vary) *MUST be licensed as a FLORIDA HISTOTECHNICIAN OR HISTOTECHNOLOGIST (NO pending licensure pls) *PREFER experience but WILL TRAIN a knowledgeable, willing candidate *duties to include (but not limited to): grossing, microtomy, accessioning, embedding and general histology *must be self motivated, reliable and a team player DAYTIME SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY: https://advancedderm.applicantpro.com/jobs/240090.html NIGHT POSITION: *Full time position Mon-Fri 10:30p-7:00a (IMMUNOHISTOCHEMISTRY/SPECIALS department) *MUST be licensed as a FLORIDA HISTOTECHNOLOGIST (this is NOT negotiable) *EXPERIENCE WITH IHC A MUST! Leica (BOND) and Roche/Ventana equipment experience preferred *Must be able to multi-task special stains *MUST have at LEAST 2 years experience. Please DO NOT respond if you do not possess EXPERIENCE in this area! *must be confidant, quick learner, self motivated, reliable and a team player NIGHT SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY: https://advancedderm.applicantpro.com/jobs/278020.html Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From rsrichmond at gmail.com Wed Sep 30 20:08:40 2015 From: rsrichmond at gmail.com (Bob Richmond) Date: Wed, 30 Sep 2015 21:08:40 -0400 Subject: [Histonet] Staples and Sutures Message-ID: When I was a resident at Johns Hopkins in the 1960s, we had a Faxitron cabinet X-ray unit, used mostly for breast specimens, but very useful for searching for metal densities in surgical specimens. It used Polaroid negative film. A digital radiographic unit is available nowadays - see http://www.faxitron.com/sites/default/files/pdf/1006-3-46%20Top-10-Cancer-Centers.pdf A pathology department wouldn't be able to convince management to spring for one of these, but it might be possible to share it with a surgical suite. Bob Richmond Samurai Pathologist Maryville TN