[Histonet] Microtome suggestions

[Hawkins, Hal K.]

The old Sorvall JB-4 microtome was designed for this purpose and works well.  They are available at a reasonable price from several used equipment providers and on Ebay. 


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From: Goetz, Freya E. via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Wednesday, October 07, 2015 11:07 AM
To: Rene J Buesa
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome suggestions

Hi Rene and histonet,

Thank you for your suggestions. I must amend my earlier request. We have an ultra microtome from RMC and a Spencer 820 microtome. I would like to section epoxy embedded material that is larger than an ultramicrotome could handle but smaller than a sledge microtome. Sorry I wasn’t specific.

Thank you again!

Freya


On Oct 7, 2015, at 12:01 PM, Rene J Buesa <rjbuesa at yahoo.com<mailto:rjbuesa at yahoo.com>> wrote:

For large specimens you need a "horizontal" or sledge microtome. Leitz (Leica) manufactures the best, but you could try an OMS from Reichert although I am not sure they stll are manufactured because Leica swallowed Reichert some years ago.
Another alternative, and probably even better than the Leitz (Leica), is a Spencer (American Optical) sliding microtome Model 860
For me this is the best all-around ever manufactured sliding microtome and not too long ago a saw one for sale in e-Bay.
Why don't you try looking for it. It will be much cheaper than any modern horizontal microtome and far better than any new one.
René J.



On Wednesday, October 7, 2015 9:08 AM, "Goetz, Freya E. via Histonet" <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>> wrote:


Good morning!
I was wondering if anyone has microtome opinions they would like to share. We have an ultramicrotome but I am looking for a microtome that could cut larger pieces of tissue embedded in plastic. I am hesitant to go with Leica because service is so expensive but I’m looking for honest opinions.

Thanks and have a nice day!

Freya
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