From fpearsa at clemson.edu Thu Oct 1 07:46:52 2015 From: fpearsa at clemson.edu (Fran Pearsall) Date: Thu, 01 Oct 2015 08:46:52 -0400 Subject: [Histonet] comments/opinions on Shandon Gemini H & E stainer References: BAY119-W45852245F9D64A71B9839D8450@phx.gbl Message-ID: <560D2B3C.2070700@clemson.edu> Hi Larry, I am going through the archives regarding the Gemini AS stainer. I am wondering if you would share with me your thoughts on this machine? We are considering this one as a purchase for our histo lab? We have other Thermo equipment and I have benn generally pleased with them? Thank you, Fran Pearsall HT ASCP From jshelley at sbpdiscovery.org Thu Oct 1 12:27:08 2015 From: jshelley at sbpdiscovery.org (John Shelley) Date: Thu, 1 Oct 2015 17:27:08 +0000 Subject: [Histonet] 2016 FSH Annual Meeting Abstract Request Message-ID: Good Morning, I know that there are many people out there that have a passion to teach what they know and we would very much like to put a call out to those who would be interested in presenting a workshop or two at our 2016 Florida Society for Histotechnology Annual meeting in the beautiful gulf coast area of St. Petersburg FL on May19-22, 2016 at the St. Pete Bayfront Hilton. We will have two and half days' worth of lecture time, starting with our popular Administrator and Supervisor Forum, the whole day HT preparedness workshop for students or those who are planning on taking their HT/HTL certification through ASCP. Please consider this great venue and submit your abstracts. We are interested in topics on special stains, IHC, In Situ, Lean, billing, safety, digital pathology, ergonomics, cytology, CAP/CLIA inspection tips, leadership and any other topic that is relevant to today's histology laboratory. Please send abstract to John Shelley at fshgrouppresident at gmail.com. I look forward to your submissions. Vendor submissions welcomed! Our plan will be to notify you by late December to early January so please keep your calendars open until you hear back from us. Kind Regards! John J Shelley 2014 - 2016 FSH President From Karen.Heckford at DignityHealth.org Thu Oct 1 12:42:57 2015 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Thu, 1 Oct 2015 10:42:57 -0700 Subject: [Histonet] Temperature logging Message-ID: Good Morning, I have been asked to monitor the room temperatures in the Histology lab, and anywhere we store paraffin blocks, slides and reagents. Some of you I know already do this. 1st Question: What is your temperature range? 2nd Question: Are you physically monitoring it daily and writing it down on a temperature chart? The reason why Question 2 was I purchased these new thermometers that I can put in the Hi and Lo. It also has where I can download it on to a scan disk so I can down load and put on a flash drive or store in the computer. I am not sure why these will not work but I am getting grief from my Lab Director that is a Med Tech. I figure if it alarms on me I can down load it and figure out the time of the out of range since I am not here on the weekend. Cannot have anyone else do it because I am the only Histology person in this hospital. The lab people do not give much support when a alarm is going off they ignore it. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From HornHV at archildrens.org Thu Oct 1 12:52:24 2015 From: HornHV at archildrens.org (Horn, Hazel V) Date: Thu, 1 Oct 2015 12:52:24 -0500 Subject: [Histonet] billing consults Message-ID: <25A4DE08332B19499904459F00AAACB71A3BC790E9@EVS1.archildrens.org> Histonetters, 1. Please see the questions about billing for Pathology Consultations under varied circumstances as denoted below. For each circumstance, please specify whether you bill, a) professional and technical only, or b) professional, technical and consult. 1. If your response choice is b), please specify whether your payors typically pay for the consult charge. ============================================================================================================== 1. The pathologist to whom the case was assigned has some doubts as to the best diagnosis after all attempts to classify the lesion. 2. The department has some disagreement about the diagnosis and sends the case to another pathologist to help decide the matter. 3. The clinician and/or patient requests a second opinion from another pathologist. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org From liz at premierlab.com Thu Oct 1 13:16:04 2015 From: liz at premierlab.com (Elizabeth Chlipala) Date: Thu, 1 Oct 2015 12:16:04 -0600 Subject: [Histonet] Temperature logging In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02230F9D95A4@SBS2K8.premierlab.local> Karen There was a similar question on temp charts the other day on the histonet. We record our temps daily on a form. Our temperature range is determined by the equipment in the room, all operating manuals have ranges listed for temp, we list all major equipment in a room and what the recommended temperature range is listed in the operating manual and make a determination of a range based upon how critical the equipment is, etc. We have an SOP that governs this and how we manage the thermometers also. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, October 01, 2015 11:43 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Temperature logging Good Morning, I have been asked to monitor the room temperatures in the Histology lab, and anywhere we store paraffin blocks, slides and reagents. Some of you I know already do this. 1st Question: What is your temperature range? 2nd Question: Are you physically monitoring it daily and writing it down on a temperature chart? The reason why Question 2 was I purchased these new thermometers that I can put in the Hi and Lo. It also has where I can download it on to a scan disk so I can down load and put on a flash drive or store in the computer. I am not sure why these will not work but I am getting grief from my Lab Director that is a Med Tech. I figure if it alarms on me I can down load it and figure out the time of the out of range since I am not here on the weekend. Cannot have anyone else do it because I am the only Histology person in this hospital. The lab people do not give much support when a alarm is going off they ignore it. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sylvain.loubery at gmail.com Fri Oct 2 02:55:56 2015 From: sylvain.loubery at gmail.com (=?UTF-8?Q?Sylvain_Loub=C3=A9ry?=) Date: Fri, 2 Oct 2015 09:55:56 +0200 Subject: [Histonet] Opinions about the Intavis InsituPro system Message-ID: Dear All, my department is considering the purchase of an Intavis InsituPro VSi system (http://intavis.com/products/automated-ish-and-ihc/insituprovsi), and I'd be very interested in any feedback I could get on this instrument. Our interests are in plant biology, for both immunofluorescence and in situ hybridization stainings, and on whole-mount samples as well as microtome or vibratome sections. Possibly to perform screens, but probably mostly for routine work. If anyone has any opinions about this system, I'd be very grateful for any piece of information I could get! (warnings, possibilities and limitations, particular things to pay attention to...) Thanks! Sylvain Sylvain Loub?ry, Ph.D. Plant Imaging Unit Department of Botany and Plant Biology University of Geneva / Sciences III Quai Ernest-Ansermet 30 1211 Geneva 4 Switzerland Phone: (022.37.)96568 sylvain.loubery at unige.ch http://biveg.unige.ch/services/imagerie.html From e.kooijman at vumc.nl Fri Oct 2 04:19:47 2015 From: e.kooijman at vumc.nl (Kooijman, Esther) Date: Fri, 2 Oct 2015 09:19:47 +0000 Subject: [Histonet] autoradiography frozen human brain sections fall off, white matter issue Message-ID: <51AEA3C85E64ED4E851909E3CCAB574CC9A84F57@SP-MX-MBX2.vumc.nl> Dear Histonetters, Does anyone of you have experience in autoradiography and could help me with the problem falling of tissue ? Or suggestions ? We have problems with falling off sections of human brain tissue. We have done the same experiments and cutting in rodent tissue without any problems. Our protocol for autoradiography : Snap frozen tissue, cut in cryo 20um sections on Superfrost plus glass, drying ON in the fridge on silica. Stored in -20. Day of the experiment. Getting them on room temperature about 45 minutes, washing/dehydrate on room temperature in 5mM Tris (HCL PH7.4) buffer, 20 minutes. About 30 minutes - an hour drying at room temperature. Then incubation with the radio tracer for 30 minutes, room temperature, just dripping the 1 mL solution to completely cover the superfrost plus glass and ditto tissue. Turning the glass to get the solution of the glass, dipping in ice cold tris washing buffer 5mM (HCL PH7.4) 1.5 minutes and here the disaster starts with falling off extensive white matter falling off...... its washing steps of only 1.5 minute. What can I do to prevent this, any idea ? I am out of clues. I have tried to dry the sections longer after cutting sections, I have tried to pre wash in either room temp or cold buffer. Hope someone can help me. Would Superfrost plus gold glass be better ? Kind Regards, Esther Kooijman Research Technician VU University Medical Center Nuclear Medicine & PET Research Email: e.kooijman at vumc.nl Amsterdam The Netherlands From histo at pathlab.us Fri Oct 2 06:59:10 2015 From: histo at pathlab.us (Histology) Date: Fri, 2 Oct 2015 11:59:10 +0000 Subject: [Histonet] GMS and Gram stains Message-ID: <566E7795BC94204D9A21539DE282AED73098F0E5@DC01.dp.local> Hi All Netters, I need to get a better GMS and Gram stain. If anyone could share their protocols where they get the solutions that would be fantastic. Thanks, Mehndi Helgren Dominion Pathology Labs Norfolk, VA (AKA Hurricane Central) :) From brett_connolly at merck.com Fri Oct 2 09:07:45 2015 From: brett_connolly at merck.com (Connolly, Brett M) Date: Fri, 2 Oct 2015 10:07:45 -0400 Subject: [Histonet] autoradiography frozen human brain sections fall off, white matter issue In-Reply-To: <51AEA3C85E64ED4E851909E3CCAB574CC9A84F57@SP-MX-MBX2.vumc.nl> References: <51AEA3C85E64ED4E851909E3CCAB574CC9A84F57@SP-MX-MBX2.vumc.nl> Message-ID: Hi Esther, We do a lot of autoradiography on rodent, primate and human brains sections. Our protocol is similar to yours except we do not dry the slides after incubating in the buffer and before adding the radiotracer. The experiment is performed with the slides in a rack and totally immersed in the solutions in staining dishes. 1. incubate slides in assay buffer 15 min. at RT 2. add radiotracer to buffer, gently mix and incubate 90-120 min. at RT 3. wash GENTLY (no agitation) in ice-cold wash buffer 3 x 3 min. We set up 3 containers and transfer the slides. 4. wash in ice-cold DI water 5 seconds. 5. air dry The Superfrost gold slides are supposed to improve adherence and would be worth a try. It could be your other Superfrost slides are old or a bad batch...which I think has been experienced by some people on the list. We do store our sectioned slides at -70C until use and then bring them up to RT the day of the experiment. They are sectioned, dried at RT for 15-20 min, and then store at -70C... No drying in the fridge. Good luck, Brett Brett M. Connolly, Ph.D. Principle Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: Kooijman, Esther via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, October 02, 2015 5:20 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] autoradiography frozen human brain sections fall off, white matter issue Importance: High Dear Histonetters, Does anyone of you have experience in autoradiography and could help me with the problem falling of tissue ? Or suggestions ? We have problems with falling off sections of human brain tissue. We have done the same experiments and cutting in rodent tissue without any problems. Our protocol for autoradiography : Snap frozen tissue, cut in cryo 20um sections on Superfrost plus glass, drying ON in the fridge on silica. Stored in -20. Day of the experiment. Getting them on room temperature about 45 minutes, washing/dehydrate on room temperature in 5mM Tris (HCL PH7.4) buffer, 20 minutes. About 30 minutes - an hour drying at room temperature. Then incubation with the radio tracer for 30 minutes, room temperature, just dripping the 1 mL solution to completely cover the superfrost plus glass and ditto tissue. Turning the glass to get the solution of the glass, dipping in ice cold tris washing buffer 5mM (HCL PH7.4) 1.5 minutes and here the disaster starts with falling off extensive white matter falling off...... its washing steps of only 1.5 minute. What can I do to prevent this, any idea ? I am out of clues. I have tried to dry the sections longer after cutting sections, I have tried to pre wash in either room temp or cold buffer. Hope someone can help me. Would Superfrost plus gold glass be better ? Kind Regards, Esther Kooijman Research Technician VU University Medical Center Nuclear Medicine & PET Research Email: e.kooijman at vumc.nl Amsterdam The Netherlands _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From Sruby at 4path.com Fri Oct 2 09:09:50 2015 From: Sruby at 4path.com (Stephen G. Ruby) Date: Fri, 2 Oct 2015 09:09:50 -0500 Subject: [Histonet] Labeling formats Message-ID: <92B59D6A-774A-428C-AD5F-A3B458CEF7D8@4path.com> I am curious on what formats that labs use for labeling. We use prefix-year-accession number-part(alpha)-block number(numeric) Example. S15-12345-A1 If you use a different format can you share it here? Thanks. Dr Ruby Sent from my mobile device From jennc976 at gmail.com Fri Oct 2 09:47:38 2015 From: jennc976 at gmail.com (Jennifer Clark) Date: Fri, 2 Oct 2015 10:47:38 -0400 Subject: [Histonet] Missing specimen Message-ID: <2C4E8BAA-B34E-4D7E-868B-FB51CC9E14D0@gmail.com> I have a situation that has occurred and am unsure how to proceed. One MA called to get results for a young patient. We had no record of a specimen for this patient at all. The doctor was sure it was performed. With some research, we discovered that another patient, middle aged,(who was in the same room with the same MA after the young patient) had two tissue pieces in his specimen bottle. This was not mentioned in the original path report, and the tissue was sent for a second opinion to confirm a malignancy. Upon re-reviewing the slide, the two specimens in the middle aged patient's block look different, one malignant, one benign. Also one showed "young skin" and the other sun damaged, more mature skin, according to the dermatologist. They are now wanting these tissues split up and a path report issued on the young patient who's specimen is missing based on these findings, with his being the benign tissue. I feel, while suspicious, I should not do this with out DNA testing to confirm. That being said, to my knowledge no other specimens are missing from that day. Please Help! Thanks! Jennifer Clark, HT Sent from my iPhone From Valerie.Hannen at parrishmed.com Fri Oct 2 09:56:11 2015 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Fri, 2 Oct 2015 10:56:11 -0400 Subject: [Histonet] Labeling formats In-Reply-To: <92B59D6A-774A-428C-AD5F-A3B458CEF7D8@4path.com> References: <92B59D6A-774A-428C-AD5F-A3B458CEF7D8@4path.com> Message-ID: <450B7A81EDA0C54E97C53D60F00776C32347EC18E1@isexstore03> Ours is very similar...only difference being ours is: 15S-1234A-1 (year-prefix-accession number-part(alpha)-block number(numeric). Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Stephen G. Ruby via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, October 02, 2015 10:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Labeling formats I am curious on what formats that labs use for labeling. We use prefix-year-accession number-part(alpha)-block number(numeric) Example. S15-12345-A1 If you use a different format can you share it here? Thanks. Dr Ruby Sent from my mobile device _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From john.frazier at roche.com Fri Oct 2 12:33:11 2015 From: john.frazier at roche.com (Frazier, John) Date: Fri, 2 Oct 2015 13:33:11 -0400 Subject: [Histonet] Labeling formats In-Reply-To: <92B59D6A-774A-428C-AD5F-A3B458CEF7D8@4path.com> References: <92B59D6A-774A-428C-AD5F-A3B458CEF7D8@4path.com> Message-ID: <-8865741258025285922@unknownmsgid> This is a format that I would recommend. To expand on that, using an "alpha" character for the part and " numeric for the cassettes makes better sense because there are few cases that have more than 26 parts (causing AA, AB, AC labeling rare), but there are many parts that have more than 26 cassettes, common. With the cassettes you can number the cassettes S15-1234-Z54 part Z(26) and the 54th cassette in the Z part. This format avoids double alpha characters up to the 27th part in the case. [image: The Hoops News] Sent from my iPhone On Oct 2, 2015, at 10:09 AM, Stephen G. Ruby wrote: I am curious on what formats that labs use for labeling. We use prefix-year-accession number-part(alpha)-block number(numeric) Example. S15-12345-A1 If you use a different format can you share it here? Thanks. Dr Ruby Sent from my mobile device From badams at acadianagastro.com Fri Oct 2 12:41:39 2015 From: badams at acadianagastro.com (Brent Adams) Date: Fri, 2 Oct 2015 17:41:39 +0000 Subject: [Histonet] Tempature Logging In-Reply-To: References: Message-ID: Karen, We were hit with minor incident by CLIA for not having Temp and Humidity documentation as well. We also use the equipment manual to set the Temp and Humidity operation ranges. WE have a hi/ lo Temp and Humidity monitor on the door jam between both rooms. We hand document the time and the Tech signs off we * any day we have out of range and write down the corrective action which is usually notified maintenance. We also only document during hours of operation. Histology does not run 24/7/365 like the clinical Lab so we have to adapt some of the rules to our operation. Our block file room has a back up A/C unit so Brent Adams ? BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Friday, October 2, 2015 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 143, Issue 2 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. 2016 FSH Annual Meeting Abstract Request (John Shelley) 2. Temperature logging (Heckford, Karen - SMMC-SF) 3. billing consults (Horn, Hazel V) 4. Re: Temperature logging (Elizabeth Chlipala) 5. Opinions about the Intavis InsituPro system (Sylvain Loub?ry) 6. autoradiography frozen human brain sections fall off, white matter issue (Kooijman, Esther) 7. GMS and Gram stains (Histology) 8. Re: autoradiography frozen human brain sections fall off, white matter issue (Connolly, Brett M) 9. Labeling formats (Stephen G. Ruby) 10. Missing specimen (Jennifer Clark) 11. Re: Labeling formats (Hannen, Valerie) ---------------------------------------------------------------------- Message: 1 Date: Thu, 1 Oct 2015 17:27:08 +0000 From: John Shelley To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] 2016 FSH Annual Meeting Abstract Request Message-ID: Content-Type: text/plain; charset="us-ascii" Good Morning, I know that there are many people out there that have a passion to teach what they know and we would very much like to put a call out to those who would be interested in presenting a workshop or two at our 2016 Florida Society for Histotechnology Annual meeting in the beautiful gulf coast area of St. Petersburg FL on May19-22, 2016 at the St. Pete Bayfront Hilton. We will have two and half days' worth of lecture time, starting with our popular Administrator and Supervisor Forum, the whole day HT preparedness workshop for students or those who are planning on taking their HT/HTL certification through ASCP. Please consider this great venue and submit your abstracts. We are interested in topics on special stains, IHC, In Situ, Lean, billing, safety, digital pathology, ergonomics, cytology, CAP/CLIA inspection tips, leadership and any other topic that is relevant to today's histology laboratory. Please send abstract to John Shelley at fshgrouppresident at gmail.com. I look forward to your submissions. Vendor submissions welcomed! Our plan will be to notify you by late December to early January so please keep your calendars open until you hear back from us. Kind Regards! John J Shelley 2014 - 2016 FSH President ------------------------------ Message: 2 Date: Thu, 1 Oct 2015 10:42:57 -0700 From: "Heckford, Karen - SMMC-SF" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Temperature logging Message-ID: Content-Type: text/plain; charset="us-ascii" Good Morning, I have been asked to monitor the room temperatures in the Histology lab, and anywhere we store paraffin blocks, slides and reagents. Some of you I know already do this. 1st Question: What is your temperature range? 2nd Question: Are you physically monitoring it daily and writing it down on a temperature chart? The reason why Question 2 was I purchased these new thermometers that I can put in the Hi and Lo. It also has where I can download it on to a scan disk so I can down load and put on a flash drive or store in the computer. I am not sure why these will not work but I am getting grief from my Lab Director that is a Med Tech. I figure if it alarms on me I can down load it and figure out the time of the out of range since I am not here on the weekend. Cannot have anyone else do it because I am the only Histology person in this hospital. The lab people do not give much support when a alarm is going off they ignore it. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ------------------------------ Message: 3 Date: Thu, 1 Oct 2015 12:52:24 -0500 From: "Horn, Hazel V" To: "histonet (histonet at lists.utsouthwestern.edu)" Subject: [Histonet] billing consults Message-ID: <25A4DE08332B19499904459F00AAACB71A3BC790E9 at EVS1.archildrens.org> Content-Type: text/plain; charset="us-ascii" Histonetters, 1. Please see the questions about billing for Pathology Consultations under varied circumstances as denoted below. For each circumstance, please specify whether you bill, a) professional and technical only, or b) professional, technical and consult. 1. If your response choice is b), please specify whether your payors typically pay for the consult charge. ============================================================================================================== 1. The pathologist to whom the case was assigned has some doubts as to the best diagnosis after all attempts to classify the lesion. 2. The department has some disagreement about the diagnosis and sends the case to another pathologist to help decide the matter. 3. The clinician and/or patient requests a second opinion from another pathologist. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org ------------------------------ Message: 4 Date: Thu, 1 Oct 2015 12:16:04 -0600 From: Elizabeth Chlipala To: "Heckford, Karen - SMMC-SF" Cc: "'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu)" Subject: Re: [Histonet] Temperature logging Message-ID: <14E2C6176416974295479C64A11CB9AE02230F9D95A4 at SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Karen There was a similar question on temp charts the other day on the histonet. We record our temps daily on a form. Our temperature range is determined by the equipment in the room, all operating manuals have ranges listed for temp, we list all major equipment in a room and what the recommended temperature range is listed in the operating manual and make a determination of a range based upon how critical the equipment is, etc. We have an SOP that governs this and how we manage the thermometers also. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, October 01, 2015 11:43 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Temperature logging Good Morning, I have been asked to monitor the room temperatures in the Histology lab, and anywhere we store paraffin blocks, slides and reagents. Some of you I know already do this. 1st Question: What is your temperature range? 2nd Question: Are you physically monitoring it daily and writing it down on a temperature chart? The reason why Question 2 was I purchased these new thermometers that I can put in the Hi and Lo. It also has where I can download it on to a scan disk so I can down load and put on a flash drive or store in the computer. I am not sure why these will not work but I am getting grief from my Lab Director that is a Med Tech. I figure if it alarms on me I can down load it and figure out the time of the out of range since I am not here on the weekend. Cannot have anyone else do it because I am the only Histology person in this hospital. The lab people do not give much support when a alarm is going off they ignore it. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 2 Oct 2015 09:55:56 +0200 From: Sylvain Loub?ry To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Opinions about the Intavis InsituPro system Message-ID: Content-Type: text/plain; charset=UTF-8 Dear All, my department is considering the purchase of an Intavis InsituPro VSi system (http://intavis.com/products/automated-ish-and-ihc/insituprovsi), and I'd be very interested in any feedback I could get on this instrument. Our interests are in plant biology, for both immunofluorescence and in situ hybridization stainings, and on whole-mount samples as well as microtome or vibratome sections. Possibly to perform screens, but probably mostly for routine work. If anyone has any opinions about this system, I'd be very grateful for any piece of information I could get! (warnings, possibilities and limitations, particular things to pay attention to...) Thanks! Sylvain Sylvain Loub?ry, Ph.D. Plant Imaging Unit Department of Botany and Plant Biology University of Geneva / Sciences III Quai Ernest-Ansermet 30 1211 Geneva 4 Switzerland Phone: (022.37.)96568 sylvain.loubery at unige.ch http://biveg.unige.ch/services/imagerie.html ------------------------------ Message: 6 Date: Fri, 2 Oct 2015 09:19:47 +0000 From: "Kooijman, Esther" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] autoradiography frozen human brain sections fall off, white matter issue Message-ID: <51AEA3C85E64ED4E851909E3CCAB574CC9A84F57 at SP-MX-MBX2.vumc.nl> Content-Type: text/plain; charset="us-ascii" Dear Histonetters, Does anyone of you have experience in autoradiography and could help me with the problem falling of tissue ? Or suggestions ? We have problems with falling off sections of human brain tissue. We have done the same experiments and cutting in rodent tissue without any problems. Our protocol for autoradiography : Snap frozen tissue, cut in cryo 20um sections on Superfrost plus glass, drying ON in the fridge on silica. Stored in -20. Day of the experiment. Getting them on room temperature about 45 minutes, washing/dehydrate on room temperature in 5mM Tris (HCL PH7.4) buffer, 20 minutes. About 30 minutes - an hour drying at room temperature. Then incubation with the radio tracer for 30 minutes, room temperature, just dripping the 1 mL solution to completely cover the superfrost plus glass and ditto tissue. Turning the glass to get the solution of the glass, dipping in ice cold tris washing buffer 5mM (HCL PH7.4) 1.5 minutes and here the disaster starts with falling off extensive white matter falling off...... its washing steps of only 1.5 minute. What can I do to prevent this, any idea ? I am out of clues. I have tried to dry the sections longer after cutting sections, I have tried to pre wash in either room temp or cold buffer. Hope someone can help me. Would Superfrost plus gold glass be better ? Kind Regards, Esther Kooijman Research Technician VU University Medical Center Nuclear Medicine & PET Research Email: e.kooijman at vumc.nl Amsterdam The Netherlands ------------------------------ Message: 7 Date: Fri, 2 Oct 2015 11:59:10 +0000 From: Histology To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] GMS and Gram stains Message-ID: <566E7795BC94204D9A21539DE282AED73098F0E5 at DC01.dp.local> Content-Type: text/plain; charset="us-ascii" Hi All Netters, I need to get a better GMS and Gram stain. If anyone could share their protocols where they get the solutions that would be fantastic. Thanks, Mehndi Helgren Dominion Pathology Labs Norfolk, VA (AKA Hurricane Central) :) ------------------------------ Message: 8 Date: Fri, 2 Oct 2015 10:07:45 -0400 From: "Connolly, Brett M" To: "Kooijman, Esther" Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] autoradiography frozen human brain sections fall off, white matter issue Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Esther, We do a lot of autoradiography on rodent, primate and human brains sections. Our protocol is similar to yours except we do not dry the slides after incubating in the buffer and before adding the radiotracer. The experiment is performed with the slides in a rack and totally immersed in the solutions in staining dishes. 1. incubate slides in assay buffer 15 min. at RT 2. add radiotracer to buffer, gently mix and incubate 90-120 min. at RT 3. wash GENTLY (no agitation) in ice-cold wash buffer 3 x 3 min. We set up 3 containers and transfer the slides. 4. wash in ice-cold DI water 5 seconds. 5. air dry The Superfrost gold slides are supposed to improve adherence and would be worth a try. It could be your other Superfrost slides are old or a bad batch...which I think has been experienced by some people on the list. We do store our sectioned slides at -70C until use and then bring them up to RT the day of the experiment. They are sectioned, dried at RT for 15-20 min, and then store at -70C... No drying in the fridge. Good luck, Brett Brett M. Connolly, Ph.D. Principle Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: Kooijman, Esther via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, October 02, 2015 5:20 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] autoradiography frozen human brain sections fall off, white matter issue Importance: High Dear Histonetters, Does anyone of you have experience in autoradiography and could help me with the problem falling of tissue ? Or suggestions ? We have problems with falling off sections of human brain tissue. We have done the same experiments and cutting in rodent tissue without any problems. Our protocol for autoradiography : Snap frozen tissue, cut in cryo 20um sections on Superfrost plus glass, drying ON in the fridge on silica. Stored in -20. Day of the experiment. Getting them on room temperature about 45 minutes, washing/dehydrate on room temperature in 5mM Tris (HCL PH7.4) buffer, 20 minutes. About 30 minutes - an hour drying at room temperature. Then incubation with the radio tracer for 30 minutes, room temperature, just dripping the 1 mL solution to completely cover the superfrost plus glass and ditto tissue. Turning the glass to get the solution of the glass, dipping in ice cold tris washing buffer 5mM (HCL PH7.4) 1.5 minutes and here the disaster starts with falling off extensive white matter falling off...... its washing steps of only 1.5 minute. What can I do to prevent this, any idea ? I am out of clues. I have tried to dry the sections longer after cutting sections, I have tried to pre wash in either room temp or cold buffer. Hope someone can help me. Would Superfrost plus gold glass be better ? Kind Regards, Esther Kooijman Research Technician VU University Medical Center Nuclear Medicine & PET Research Email: e.kooijman at vumc.nl Amsterdam The Netherlands _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 9 Date: Fri, 2 Oct 2015 09:09:50 -0500 From: "Stephen G. Ruby" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Labeling formats Message-ID: <92B59D6A-774A-428C-AD5F-A3B458CEF7D8 at 4path.com> Content-Type: text/plain; charset="us-ascii" I am curious on what formats that labs use for labeling. We use prefix-year-accession number-part(alpha)-block number(numeric) Example. S15-12345-A1 If you use a different format can you share it here? Thanks. Dr Ruby Sent from my mobile device ------------------------------ Message: 10 Date: Fri, 2 Oct 2015 10:47:38 -0400 From: Jennifer Clark To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Missing specimen Message-ID: <2C4E8BAA-B34E-4D7E-868B-FB51CC9E14D0 at gmail.com> Content-Type: text/plain; charset=us-ascii I have a situation that has occurred and am unsure how to proceed. One MA called to get results for a young patient. We had no record of a specimen for this patient at all. The doctor was sure it was performed. With some research, we discovered that another patient, middle aged,(who was in the same room with the same MA after the young patient) had two tissue pieces in his specimen bottle. This was not mentioned in the original path report, and the tissue was sent for a second opinion to confirm a malignancy. Upon re-reviewing the slide, the two specimens in the middle aged patient's block look different, one malignant, one benign. Also one showed "young skin" and the other sun damaged, more mature skin, according to the dermatologist. They are now wanting these tissues split up and a path report issued on the young patient who's specimen is missing based on these findings, with his being the benign tissue. I feel, while suspicious, I should not do this with out DNA testing to confirm. That being said, to my knowledge no other specimens are missing from that day. Please Help! Thanks! Jennifer Clark, HT Sent from my iPhone ------------------------------ Message: 11 Date: Fri, 2 Oct 2015 10:56:11 -0400 From: "Hannen, Valerie" To: "'Stephen G. Ruby'" Cc: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Labeling formats Message-ID: <450B7A81EDA0C54E97C53D60F00776C32347EC18E1 at isexstore03> Content-Type: text/plain; charset="us-ascii" Ours is very similar...only difference being ours is: 15S-1234A-1 (year-prefix-accession number-part(alpha)-block number(numeric). Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Stephen G. Ruby via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, October 02, 2015 10:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Labeling formats I am curious on what formats that labs use for labeling. We use prefix-year-accession number-part(alpha)-block number(numeric) Example. S15-12345-A1 If you use a different format can you share it here? Thanks. Dr Ruby Sent from my mobile device _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 143, Issue 2 **************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. From john.frazier at roche.com Fri Oct 2 12:48:21 2015 From: john.frazier at roche.com (Frazier, John) Date: Fri, 2 Oct 2015 13:48:21 -0400 Subject: [Histonet] Labeling formats In-Reply-To: <92B59D6A-774A-428C-AD5F-A3B458CEF7D8@4path.com> References: <92B59D6A-774A-428C-AD5F-A3B458CEF7D8@4path.com> Message-ID: <-770841560089713187@unknownmsgid> Additionally, we are all moving to a digital world of accessioning and labeling. The days of leaving out the "A" or "1" to indicate that there is only one part or one cassette need to become a process of the past. LIS as sighed labeling can not accommodate this format. Sent from my iPhone > On Oct 2, 2015, at 10:09 AM, Stephen G. Ruby wrote: > > I am curious on what formats that labs use for labeling. > We use prefix-year-accession number-part(alpha)-block number(numeric) > Example. S15-12345-A1 > > If you use a different format can you share it here? > Thanks. > Dr Ruby > > Sent from my mobile device > > From john.frazier at roche.com Fri Oct 2 12:54:33 2015 From: john.frazier at roche.com (Frazier, John) Date: Fri, 2 Oct 2015 13:54:33 -0400 Subject: [Histonet] Missing specimen In-Reply-To: <2C4E8BAA-B34E-4D7E-868B-FB51CC9E14D0@gmail.com> References: <2C4E8BAA-B34E-4D7E-868B-FB51CC9E14D0@gmail.com> Message-ID: <6468967756908786061@unknownmsgid> You can not speculate. You have no chose but to wait on the DNA results. This could become a legal case at some point so there is good reason to not speculate but have indisputable proof Sent from my iPhone > On Oct 2, 2015, at 10:47 AM, Jennifer Clark wrote: > > > I have a situation that has occurred and am unsure how to proceed. One MA called to get results for a young patient. We had no record of a specimen for this patient at all. The doctor was sure it was performed. With some research, we discovered that another patient, middle aged,(who was in the same room with the same MA after the young patient) had two tissue pieces in his specimen bottle. This was not mentioned in the original path report, and the tissue was sent for a second opinion to confirm a malignancy. Upon re-reviewing the slide, the two specimens in the middle aged patient's block look different, one malignant, one benign. Also one showed "young skin" and the other sun damaged, more mature skin, according to the dermatologist. > They are now wanting these tissues split up and a path report issued on the young patient who's specimen is missing based on these findings, with his being the benign tissue. I feel, while suspicious, I should not do this with out DNA testing to confirm. That being said, to my knowledge no other specimens are missing from that day. Please Help! > > Thanks! > Jennifer Clark, HT > > Sent from my iPhone > From HernandezJW at uthscsa.edu Fri Oct 2 13:42:39 2015 From: HernandezJW at uthscsa.edu (Hernandez, Jesus Willibaldo) Date: Fri, 2 Oct 2015 18:42:39 +0000 Subject: [Histonet] Acid Phosphatase, Leukocyte (TRAP) Kit (387A SIGMA) Message-ID: I was having issues using this TRAP Staining Kit for osteoclast activity. I followed the protocol, but did not counterstain. I checked the slides and saw no reactivity. I did notice golden cluster of cells within the bone marrow, but nothing red/purple. I am thinking that the (EDTA) decal process might of ate away the enzyme in the samples. As of right now, I am placing the slides back into the TRAP solutions to have more time to react and potentially give us that red/purple color. Any guidance would be appreciated. -JH From kdonadio at ymail.com Fri Oct 2 15:00:32 2015 From: kdonadio at ymail.com (Kim Donadio) Date: Fri, 2 Oct 2015 13:00:32 -0700 Subject: [Histonet] Missing specimen In-Reply-To: <6468967756908786061@unknownmsgid> Message-ID: <1443816032.29134.YahooMailMobile@web121903.mail.ne1.yahoo.com> Stand your ground. You're right! Patients lives are not guessing games. I wish you the best and must forewarn you. Choosing integrity in this day and age is a lonely road and often doesn't pay well. But your conscious will be clear and it's the right thing to do. From kdonadio at ymail.com Fri Oct 2 15:09:22 2015 From: kdonadio at ymail.com (Kim Donadio) Date: Fri, 2 Oct 2015 13:09:22 -0700 Subject: [Histonet] Labeling formats In-Reply-To: <-770841560089713187@unknownmsgid> Message-ID: <1443816562.12144.YahooMailMobile@web121904.mail.ne1.yahoo.com> Dr Ruby, All places I've worked at use similar as well. Also you need a second patient identifier as blocks and slides are considered an aliquot from the original specimen. Such as last name, date of birth etc And it doesn't have to be S, it can be any prefix meaningful to your specific lab. As you may have multiple offices sending you specimens and might want to designate something specific to seperate them, such as NS- # for a north office and SS for a south as a example. Kim From a.tolentino82 at gmail.com Fri Oct 2 17:15:19 2015 From: a.tolentino82 at gmail.com (Aimee Tolentino) Date: Fri, 2 Oct 2015 15:15:19 -0700 Subject: [Histonet] Missing specimen In-Reply-To: <1443816032.29134.YahooMailMobile@web121903.mail.ne1.yahoo.com> References: <1443816032.29134.YahooMailMobile@web121903.mail.ne1.yahoo.com> Message-ID: I've had something like this occur before. Unfortunately things did not end well. The prostate needle biopsies were labeled for two separate patients were labeled the same accession number and patients name. After playing the guessing game and sent those results out without a DNA they had removed a perfectly healthy prostate from the patient who did not have prostate cancer while the other had not received treatment. We later found this out through DNA after the healthy patient had gone through surgery for them to realize he didn't have cancer. So you MUST do the DNA. Sent from my iPhone > On Oct 2, 2015, at 1:00 PM, Kim Donadio via Histonet wrote: > > Stand your ground. You're right! Patients lives are not guessing games. I wish you the best and must forewarn you. Choosing integrity in this day and age is a lonely road and often doesn't pay well. But your conscious will be clear and it's the right thing to do. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sruby at 4path.com Fri Oct 2 17:23:10 2015 From: Sruby at 4path.com (Stephen G. Ruby) Date: Fri, 2 Oct 2015 17:23:10 -0500 Subject: [Histonet] Labeling formats In-Reply-To: <1443816562.12144.YahooMailMobile@web121904.mail.ne1.yahoo.com> References: <1443816562.12144.YahooMailMobile@web121904.mail.ne1.yahoo.com> Message-ID: Thanks to all for your feedback. If anyone else has different formats, please let me know. Also?.is anyone out there printing patient NAMES on the blocks? Just curious? THanks Dr. Ruby On Oct 2, 2015, at 3:09 PM, Kim Donadio > wrote: Dr Ruby, All places I've worked at use similar as well. Also you need a second patient identifier as blocks and slides are considered an aliquot from the original specimen. Such as last name, date of birth etc And it doesn't have to be S, it can be any prefix meaningful to your specific lab. As you may have multiple offices sending you specimens and might want to designate something specific to seperate them, such as NS- # for a north office and SS for a south as a example. Kim ________________________________ From: Frazier, John via Histonet >; To: Stephen G. Ruby >; Cc: histonet at lists.utsouthwestern.edu >; Subject: Re: [Histonet] Labeling formats Sent: Fri, Oct 2, 2015 5:48:21 PM Additionally, we are all moving to a digital world of accessioning and labeling. The days of leaving out the "A" or "1" to indicate that there is only one part or one cassette need to become a process of the past. LIS as sighed labeling can not accommodate this format. Sent from my iPhone > On Oct 2, 2015, at 10:09 AM, Stephen G. Ruby > wrote: > > I am curious on what formats that labs use for labeling. > We use prefix-year-accession number-part(alpha)-block number(numeric) > Example. S15-12345-A1 > > If you use a different format can you share it here? > Thanks. > Dr Ruby > > Sent from my mobile device > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Mon Oct 5 08:55:27 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 5 Oct 2015 13:55:27 +0000 Subject: [Histonet] FW: GMS and Gram stains In-Reply-To: <566E7795BC94204D9A21539DE282AED73098F1DD@DC01.dp.local> References: <566E7795BC94204D9A21539DE282AED73098F0E5@DC01.dp.local> <761E2B5697F795489C8710BCC72141FF60319638@ex07.net.ucsf.edu>, <566E7795BC94204D9A21539DE282AED73098F1DD@DC01.dp.local> Message-ID: <761E2B5697F795489C8710BCC72141FF60319D8A@ex07.net.ucsf.edu> ________________________________________ From: Histology [histo at pathlab.us] Sent: Monday, October 05, 2015 5:18 AM To: Morken, Timothy Subject: RE: GMS and Gram stains 1. Gram: Sigma-Aldrich Accustain 2. Gram: Hard to see gram negative 1. GMS: Grocott's Methenmmine Silver modified 2. GMS: Switched from microwave to oven. Too long (2 hours) and can't seem to get the fungi dark enough. Thanks -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Friday, October 02, 2015 12:16 PM To: Histology Subject: RE: GMS and Gram stains Mehndi, These procedures are so common that without more information you are likely to get a lot of people sending you procedures that are identical to what you now use. The questions we will ask are: 1) what procedures are using now? and 2) what is it about your current procedures that is not acceptable? Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Histology via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, October 02, 2015 4:59 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] GMS and Gram stains Hi All Netters, I need to get a better GMS and Gram stain. If anyone could share their protocols where they get the solutions that would be fantastic. Thanks, Mehndi Helgren Dominion Pathology Labs Norfolk, VA (AKA Hurricane Central) :) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV at archildrens.org Mon Oct 5 10:24:22 2015 From: HornHV at archildrens.org (Horn, Hazel V) Date: Mon, 5 Oct 2015 10:24:22 -0500 Subject: [Histonet] FW: billing consults In-Reply-To: <25A4DE08332B19499904459F00AAACB71A3BC790E9@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB71A3BC790E9@EVS1.archildrens.org> Message-ID: <25A4DE08332B19499904459F00AAACB71A3BC790F9@EVS1.archildrens.org> _Please respond privately if you would rather. If you have billed for the professional component, do you bill the patient again for the consultation if you have sent it out to a pathologist for confirmation or diagnosis? __________________________________________________________________ From: Horn, Hazel V via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, October 01, 2015 12:52 PM To: histonet (histonet at lists.utsouthwestern.edu) Subject: [Histonet] billing consults Histonetters, 1. Please see the questions about billing for Pathology Consultations under varied circumstances as denoted below. For each circumstance, please specify whether you bill, a) professional and technical only, or b) professional, technical and consult. 1. If your response choice is b), please specify whether your payors typically pay for the consult charge. ============================================================================================================== 1. The pathologist to whom the case was assigned has some doubts as to the best diagnosis after all attempts to classify the lesion. 2. The department has some disagreement about the diagnosis and sends the case to another pathologist to help decide the matter. 3. The clinician and/or patient requests a second opinion from another pathologist. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Caroline.Pratt at uphs.upenn.edu Mon Oct 5 10:30:52 2015 From: Caroline.Pratt at uphs.upenn.edu (Pratt, Caroline) Date: Mon, 5 Oct 2015 15:30:52 +0000 Subject: [Histonet] Missing specimen In-Reply-To: References: <1443816032.29134.YahooMailMobile@web121903.mail.ne1.yahoo.com>, Message-ID: <14D6A469D20B4F4AACC47C3E973C3FA8DDF843@UPHMASPHI030.UPHS.PENNHEALTH.PRV> I agree with using caution, but these are not truly comparable as a Pathologist can tell the difference between a child's skin and the skin of a mature adult under the microscope. ________________________________________ From: Aimee Tolentino via Histonet [histonet at lists.utsouthwestern.edu] Sent: Friday, October 02, 2015 6:15 PM To: Kim Donadio Cc: Frazier, John; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Missing specimen I've had something like this occur before. Unfortunately things did not end well. The prostate needle biopsies were labeled for two separate patients were labeled the same accession number and patients name. After playing the guessing game and sent those results out without a DNA they had removed a perfectly healthy prostate from the patient who did not have prostate cancer while the other had not received treatment. We later found this out through DNA after the healthy patient had gone through surgery for them to realize he didn't have cancer. So you MUST do the DNA. Sent from my iPhone > On Oct 2, 2015, at 1:00 PM, Kim Donadio via Histonet wrote: > > Stand your ground. You're right! Patients lives are not guessing games. I wish you the best and must forewarn you. Choosing integrity in this day and age is a lonely road and often doesn't pay well. But your conscious will be clear and it's the right thing to do. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From certndx at gmail.com Mon Oct 5 14:04:43 2015 From: certndx at gmail.com (Andy B) Date: Mon, 5 Oct 2015 15:04:43 -0400 Subject: [Histonet] TC labs Message-ID: Does CLIA inspect labs that only perform Technical Components on tissue? I know that CAP does so as part of its due diligence, however years ago a CLIA inspector told me that CLIA doesnt inspect TC-only operations because there is no report (PC) being rendered. How does this jive with grossing and the new High Complexity Testing (HCT) paradigm? In other words, what is to prevent a pathologist (or non-pathologist) from owning and operating a TC lab service that is remote from the PC service location? How does CMS verify that TC labs are correctly and compliantly performing grossing (HCT) and other TC lab evolutions if they do not inspect such operations? I know of labs that are TC only and bill CMS for the Technical Components. Has anyone else ever wondered about this? How is CMS assuring that TC labs are compliantly staffed and managed if CMS only inspects labs that issue reports? Does anyone wonder how CMS can get away with hammering some labs and ignoring others? From Timothy.Morken at ucsf.edu Mon Oct 5 14:28:34 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 5 Oct 2015 19:28:34 +0000 Subject: [Histonet] TC labs In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF60319FA1@ex07.net.ucsf.edu> What I believe happens is that the referring lab must do an audit/inspection of the technical-only lab to be sure they are CLIA compliant. For instance, check validation procedures for stains, equipment, quality control. It's true CLIA itself does not inspect the Tech-only lab, but the referring lab becomes the "technical supervisor" and must have documentation to show they have inspected it and it is compliant. That is how it works for vendors using other labs to do work for them under FDA regulations. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Andy B via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, October 05, 2015 12:05 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] TC labs Does CLIA inspect labs that only perform Technical Components on tissue? I know that CAP does so as part of its due diligence, however years ago a CLIA inspector told me that CLIA doesnt inspect TC-only operations because there is no report (PC) being rendered. How does this jive with grossing and the new High Complexity Testing (HCT) paradigm? In other words, what is to prevent a pathologist (or non-pathologist) from owning and operating a TC lab service that is remote from the PC service location? How does CMS verify that TC labs are correctly and compliantly performing grossing (HCT) and other TC lab evolutions if they do not inspect such operations? I know of labs that are TC only and bill CMS for the Technical Components. Has anyone else ever wondered about this? How is CMS assuring that TC labs are compliantly staffed and managed if CMS only inspects labs that issue reports? Does anyone wonder how CMS can get away with hammering some labs and ignoring others? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From certndx at gmail.com Mon Oct 5 14:47:13 2015 From: certndx at gmail.com (Andy B) Date: Mon, 5 Oct 2015 15:47:13 -0400 Subject: [Histonet] TC labs In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF60319FA1@ex07.net.ucsf.edu> Message-ID: Thank you. That is exactly what I would have given as an answer, but I have yet to see any CLIA inspector or CLIA Operations manual that mentions any of that, other than the vague statements about the Lab Director ultimately being responsible. CLIA is not consistent with its rules and regulations and interpretations. I would love for someone to prove me wrong! When is the last time someone actually witnessed CLIA verifying a TC lab other than maybe being shown some QC logs, etc? I have yet to see any Testing Personnel verifications from CLIA for TC labs. It is hypocritical for CLIA not to inspect TC- only operations. If there is a standard form or regulation for this, please show me. I have never seen or heard of a PC only lab director being asked for verification of testing personnel qualifications from a TC lab. Thanks in advance for everyone's input. On Mon, Oct 5, 2015 at 3:42 PM, Andy B wrote: > Thank you. That is exactly what I would have given as an answer, but I > have yet to see any CLIA inspector or CLIA Operations manual that mentions > any of that, other than the vague statements about the Lab Director > ultimately being responsible. CLIA is not consistent with its rules and > regulations and interpretations. I would love for someone to prove me wrong! > > When is the last time someone actually witnessed CLIA verifying a TC lab > other than maybe being shown some QC logs, etc? I have yet to see any > Testing Personnel verifications from CLIA for TC labs. It is hypocritical > for CLIA not to inspect TC- only operations. If there is a standard form or > regulation for this, please show me. I have never seen or heard of a PC > only lab director being asked for verification of testing personnel > qualifications from a TC lab. > > Thanks in advance for everyone's input. > > > > On Mon, Oct 5, 2015 at 3:28 PM, Morken, Timothy > wrote: > >> What I believe happens is that the referring lab must do an >> audit/inspection of the technical-only lab to be sure they are CLIA >> compliant. For instance, check validation procedures for stains, equipment, >> quality control. It's true CLIA itself does not inspect the Tech-only lab, >> but the referring lab becomes the "technical supervisor" and must have >> documentation to show they have inspected it and it is compliant. That is >> how it works for vendors using other labs to do work for them under FDA >> regulations. >> >> >> Tim Morken >> Pathology Site Manager, Parnassus >> Supervisor, Electron Microscopy/Neuromuscular Special Studies >> Department of Pathology >> UC San Francisco Medical Center >> >> >> >> -----Original Message----- >> From: Andy B via Histonet [mailto:histonet at lists.utsouthwestern.edu] >> Sent: Monday, October 05, 2015 12:05 PM >> To: histonet at lists.utsouthwestern.edu >> Subject: [Histonet] TC labs >> >> Does CLIA inspect labs that only perform Technical Components on tissue? >> >> I know that CAP does so as part of its due diligence, however years ago a >> CLIA inspector told me that CLIA doesnt inspect TC-only operations because >> there is no report (PC) being rendered. How does this jive with grossing >> and the new High Complexity Testing (HCT) paradigm? >> >> In other words, what is to prevent a pathologist (or non-pathologist) >> from owning and operating a TC lab service that is remote from the PC >> service location? How does CMS verify that TC labs are correctly and >> compliantly performing grossing (HCT) and other TC lab evolutions if they >> do not inspect such operations? >> >> I know of labs that are TC only and bill CMS for the Technical Components. >> Has anyone else ever wondered about this? How is CMS assuring that TC >> labs are compliantly staffed and managed if CMS only inspects labs that >> issue reports? >> >> Does anyone wonder how CMS can get away with hammering some labs and >> ignoring others? >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > From DMerrill at Cheshire-Med.Com Mon Oct 5 15:06:02 2015 From: DMerrill at Cheshire-Med.Com (Merrill, Dawn S) Date: Mon, 5 Oct 2015 20:06:02 +0000 Subject: [Histonet] (no subject) Message-ID: Can you please unsubscribe me to this mailing list. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From lora at dpallp.net Mon Oct 5 15:08:04 2015 From: lora at dpallp.net (Lora Jauregui) Date: Mon, 5 Oct 2015 20:08:04 +0000 Subject: [Histonet] Unsubscribe Message-ID: Please unsubscribe me from this list. Thank you Lora Jauregui Lab Manager Diagnostic Pathology Associates 409-842-8222 lora at dpallp.net From DSiena at statlab.com Mon Oct 5 15:13:28 2015 From: DSiena at statlab.com (Debra Siena) Date: Mon, 5 Oct 2015 20:13:28 +0000 Subject: [Histonet] TC labs In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF60319FA1@ex07.net.ucsf.edu> , Message-ID: <478829E1-4373-45AF-9CBD-7B96B4C5E2CD@statlab.com> If the lab performs gross examination in any way, the lab must be considered high complexity which puts the lab under the umbrella of CLIA and must be inspected every two years. Sent from my iPhone > On Oct 5, 2015, at 3:54 PM, Andy B via Histonet wrote: > > Thank you. That is exactly what I would have given as an answer, but I have > yet to see any CLIA inspector or CLIA Operations manual that mentions any > of that, other than the vague statements about the Lab Director ultimately > being responsible. CLIA is not consistent with its rules and regulations > and interpretations. I would love for someone to prove me wrong! > > When is the last time someone actually witnessed CLIA verifying a TC lab > other than maybe being shown some QC logs, etc? I have yet to see any > Testing Personnel verifications from CLIA for TC labs. It is hypocritical > for CLIA not to inspect TC- only operations. If there is a standard form or > regulation for this, please show me. I have never seen or heard of a PC > only lab director being asked for verification of testing personnel > qualifications from a TC lab. > > Thanks in advance for everyone's input. > >> On Mon, Oct 5, 2015 at 3:42 PM, Andy B wrote: >> >> Thank you. That is exactly what I would have given as an answer, but I >> have yet to see any CLIA inspector or CLIA Operations manual that mentions >> any of that, other than the vague statements about the Lab Director >> ultimately being responsible. CLIA is not consistent with its rules and >> regulations and interpretations. I would love for someone to prove me wrong! >> >> When is the last time someone actually witnessed CLIA verifying a TC lab >> other than maybe being shown some QC logs, etc? I have yet to see any >> Testing Personnel verifications from CLIA for TC labs. It is hypocritical >> for CLIA not to inspect TC- only operations. If there is a standard form or >> regulation for this, please show me. I have never seen or heard of a PC >> only lab director being asked for verification of testing personnel >> qualifications from a TC lab. >> >> Thanks in advance for everyone's input. >> >> >> >> On Mon, Oct 5, 2015 at 3:28 PM, Morken, Timothy >> wrote: >> >>> What I believe happens is that the referring lab must do an >>> audit/inspection of the technical-only lab to be sure they are CLIA >>> compliant. For instance, check validation procedures for stains, equipment, >>> quality control. It's true CLIA itself does not inspect the Tech-only lab, >>> but the referring lab becomes the "technical supervisor" and must have >>> documentation to show they have inspected it and it is compliant. That is >>> how it works for vendors using other labs to do work for them under FDA >>> regulations. >>> >>> >>> Tim Morken >>> Pathology Site Manager, Parnassus >>> Supervisor, Electron Microscopy/Neuromuscular Special Studies >>> Department of Pathology >>> UC San Francisco Medical Center >>> >>> >>> >>> -----Original Message----- >>> From: Andy B via Histonet [mailto:histonet at lists.utsouthwestern.edu] >>> Sent: Monday, October 05, 2015 12:05 PM >>> To: histonet at lists.utsouthwestern.edu >>> Subject: [Histonet] TC labs >>> >>> Does CLIA inspect labs that only perform Technical Components on tissue? >>> >>> I know that CAP does so as part of its due diligence, however years ago a >>> CLIA inspector told me that CLIA doesnt inspect TC-only operations because >>> there is no report (PC) being rendered. How does this jive with grossing >>> and the new High Complexity Testing (HCT) paradigm? >>> >>> In other words, what is to prevent a pathologist (or non-pathologist) >>> from owning and operating a TC lab service that is remote from the PC >>> service location? How does CMS verify that TC labs are correctly and >>> compliantly performing grossing (HCT) and other TC lab evolutions if they >>> do not inspect such operations? >>> >>> I know of labs that are TC only and bill CMS for the Technical Components. >>> Has anyone else ever wondered about this? How is CMS assuring that TC >>> labs are compliantly staffed and managed if CMS only inspects labs that >>> issue reports? >>> >>> Does anyone wonder how CMS can get away with hammering some labs and >>> ignoring others? >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pickadel07 at gmail.com Mon Oct 5 15:20:14 2015 From: pickadel07 at gmail.com (Angela Davis) Date: Mon, 5 Oct 2015 16:20:14 -0400 Subject: [Histonet] (no subject) Message-ID: Pls unsubscribe me Thank you! From certndx at gmail.com Mon Oct 5 15:34:54 2015 From: certndx at gmail.com (Cert NDx) Date: Mon, 5 Oct 2015 16:34:54 -0400 Subject: [Histonet] TC labs In-Reply-To: <478829E1-4373-45AF-9CBD-7B96B4C5E2CD@statlab.com> References: <761E2B5697F795489C8710BCC72141FF60319FA1@ex07.net.ucsf.edu> <478829E1-4373-45AF-9CBD-7B96B4C5E2CD@statlab.com> Message-ID: I understand that to be the case as well. But in the case of TC only laboratories where no full report is being rendered I have yet to see any evidence that CLIA knocks on their doors. On Oct 5, 2015 4:13 PM, "Debra Siena" wrote: > If the lab performs gross examination in any way, the lab must be > considered high complexity which puts the lab under the umbrella of CLIA > and must be inspected every two years. > > Sent from my iPhone > > > On Oct 5, 2015, at 3:54 PM, Andy B via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > > Thank you. That is exactly what I would have given as an answer, but I > have > > yet to see any CLIA inspector or CLIA Operations manual that mentions any > > of that, other than the vague statements about the Lab Director > ultimately > > being responsible. CLIA is not consistent with its rules and regulations > > and interpretations. I would love for someone to prove me wrong! > > > > When is the last time someone actually witnessed CLIA verifying a TC lab > > other than maybe being shown some QC logs, etc? I have yet to see any > > Testing Personnel verifications from CLIA for TC labs. It is hypocritical > > for CLIA not to inspect TC- only operations. If there is a standard form > or > > regulation for this, please show me. I have never seen or heard of a PC > > only lab director being asked for verification of testing personnel > > qualifications from a TC lab. > > > > Thanks in advance for everyone's input. > > > >> On Mon, Oct 5, 2015 at 3:42 PM, Andy B wrote: > >> > >> Thank you. That is exactly what I would have given as an answer, but I > >> have yet to see any CLIA inspector or CLIA Operations manual that > mentions > >> any of that, other than the vague statements about the Lab Director > >> ultimately being responsible. CLIA is not consistent with its rules and > >> regulations and interpretations. I would love for someone to prove me > wrong! > >> > >> When is the last time someone actually witnessed CLIA verifying a TC lab > >> other than maybe being shown some QC logs, etc? I have yet to see any > >> Testing Personnel verifications from CLIA for TC labs. It is > hypocritical > >> for CLIA not to inspect TC- only operations. If there is a standard > form or > >> regulation for this, please show me. I have never seen or heard of a PC > >> only lab director being asked for verification of testing personnel > >> qualifications from a TC lab. > >> > >> Thanks in advance for everyone's input. > >> > >> > >> > >> On Mon, Oct 5, 2015 at 3:28 PM, Morken, Timothy < > Timothy.Morken at ucsf.edu> > >> wrote: > >> > >>> What I believe happens is that the referring lab must do an > >>> audit/inspection of the technical-only lab to be sure they are CLIA > >>> compliant. For instance, check validation procedures for stains, > equipment, > >>> quality control. It's true CLIA itself does not inspect the Tech-only > lab, > >>> but the referring lab becomes the "technical supervisor" and must have > >>> documentation to show they have inspected it and it is compliant. That > is > >>> how it works for vendors using other labs to do work for them under FDA > >>> regulations. > >>> > >>> > >>> Tim Morken > >>> Pathology Site Manager, Parnassus > >>> Supervisor, Electron Microscopy/Neuromuscular Special Studies > >>> Department of Pathology > >>> UC San Francisco Medical Center > >>> > >>> > >>> > >>> -----Original Message----- > >>> From: Andy B via Histonet [mailto:histonet at lists.utsouthwestern.edu] > >>> Sent: Monday, October 05, 2015 12:05 PM > >>> To: histonet at lists.utsouthwestern.edu > >>> Subject: [Histonet] TC labs > >>> > >>> Does CLIA inspect labs that only perform Technical Components on > tissue? > >>> > >>> I know that CAP does so as part of its due diligence, however years > ago a > >>> CLIA inspector told me that CLIA doesnt inspect TC-only operations > because > >>> there is no report (PC) being rendered. How does this jive with > grossing > >>> and the new High Complexity Testing (HCT) paradigm? > >>> > >>> In other words, what is to prevent a pathologist (or non-pathologist) > >>> from owning and operating a TC lab service that is remote from the PC > >>> service location? How does CMS verify that TC labs are correctly and > >>> compliantly performing grossing (HCT) and other TC lab evolutions if > they > >>> do not inspect such operations? > >>> > >>> I know of labs that are TC only and bill CMS for the Technical > Components. > >>> Has anyone else ever wondered about this? How is CMS assuring that TC > >>> labs are compliantly staffed and managed if CMS only inspects labs that > >>> issue reports? > >>> > >>> Does anyone wonder how CMS can get away with hammering some labs and > >>> ignoring others? > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet at lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From DSiena at statlab.com Mon Oct 5 15:37:21 2015 From: DSiena at statlab.com (Debra Siena) Date: Mon, 5 Oct 2015 20:37:21 +0000 Subject: [Histonet] TC labs In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF60319FA1@ex07.net.ucsf.edu> <478829E1-4373-45AF-9CBD-7B96B4C5E2CD@statlab.com>, Message-ID: They do on my lab which is TC only. Debbie Siena Sent from my iPhone On Oct 5, 2015, at 4:34 PM, Cert NDx > wrote: I understand that to be the case as well. But in the case of TC only laboratories where no full report is being rendered I have yet to see any evidence that CLIA knocks on their doors. On Oct 5, 2015 4:13 PM, "Debra Siena" > wrote: If the lab performs gross examination in any way, the lab must be considered high complexity which puts the lab under the umbrella of CLIA and must be inspected every two years. Sent from my iPhone > On Oct 5, 2015, at 3:54 PM, Andy B via Histonet > wrote: > > Thank you. That is exactly what I would have given as an answer, but I have > yet to see any CLIA inspector or CLIA Operations manual that mentions any > of that, other than the vague statements about the Lab Director ultimately > being responsible. CLIA is not consistent with its rules and regulations > and interpretations. I would love for someone to prove me wrong! > > When is the last time someone actually witnessed CLIA verifying a TC lab > other than maybe being shown some QC logs, etc? I have yet to see any > Testing Personnel verifications from CLIA for TC labs. It is hypocritical > for CLIA not to inspect TC- only operations. If there is a standard form or > regulation for this, please show me. I have never seen or heard of a PC > only lab director being asked for verification of testing personnel > qualifications from a TC lab. > > Thanks in advance for everyone's input. > >> On Mon, Oct 5, 2015 at 3:42 PM, Andy B > wrote: >> >> Thank you. That is exactly what I would have given as an answer, but I >> have yet to see any CLIA inspector or CLIA Operations manual that mentions >> any of that, other than the vague statements about the Lab Director >> ultimately being responsible. CLIA is not consistent with its rules and >> regulations and interpretations. I would love for someone to prove me wrong! >> >> When is the last time someone actually witnessed CLIA verifying a TC lab >> other than maybe being shown some QC logs, etc? I have yet to see any >> Testing Personnel verifications from CLIA for TC labs. It is hypocritical >> for CLIA not to inspect TC- only operations. If there is a standard form or >> regulation for this, please show me. I have never seen or heard of a PC >> only lab director being asked for verification of testing personnel >> qualifications from a TC lab. >> >> Thanks in advance for everyone's input. >> >> >> >> On Mon, Oct 5, 2015 at 3:28 PM, Morken, Timothy > >> wrote: >> >>> What I believe happens is that the referring lab must do an >>> audit/inspection of the technical-only lab to be sure they are CLIA >>> compliant. For instance, check validation procedures for stains, equipment, >>> quality control. It's true CLIA itself does not inspect the Tech-only lab, >>> but the referring lab becomes the "technical supervisor" and must have >>> documentation to show they have inspected it and it is compliant. That is >>> how it works for vendors using other labs to do work for them under FDA >>> regulations. >>> >>> >>> Tim Morken >>> Pathology Site Manager, Parnassus >>> Supervisor, Electron Microscopy/Neuromuscular Special Studies >>> Department of Pathology >>> UC San Francisco Medical Center >>> >>> >>> >>> -----Original Message----- >>> From: Andy B via Histonet [mailto:histonet at lists.utsouthwestern.edu] >>> Sent: Monday, October 05, 2015 12:05 PM >>> To: histonet at lists.utsouthwestern.edu >>> Subject: [Histonet] TC labs >>> >>> Does CLIA inspect labs that only perform Technical Components on tissue? >>> >>> I know that CAP does so as part of its due diligence, however years ago a >>> CLIA inspector told me that CLIA doesnt inspect TC-only operations because >>> there is no report (PC) being rendered. How does this jive with grossing >>> and the new High Complexity Testing (HCT) paradigm? >>> >>> In other words, what is to prevent a pathologist (or non-pathologist) >>> from owning and operating a TC lab service that is remote from the PC >>> service location? How does CMS verify that TC labs are correctly and >>> compliantly performing grossing (HCT) and other TC lab evolutions if they >>> do not inspect such operations? >>> >>> I know of labs that are TC only and bill CMS for the Technical Components. >>> Has anyone else ever wondered about this? How is CMS assuring that TC >>> labs are compliantly staffed and managed if CMS only inspects labs that >>> issue reports? >>> >>> Does anyone wonder how CMS can get away with hammering some labs and >>> ignoring others? >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kkienitz at orclinic.com Mon Oct 5 15:51:23 2015 From: kkienitz at orclinic.com (Kienitz, Kari) Date: Mon, 5 Oct 2015 13:51:23 -0700 Subject: [Histonet] TC labs In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF60319FA1@ex07.net.ucsf.edu> , Message-ID: <41400FFE517878449D89114DD2526090196602D283@tocmail1.tocad.orclinic.com> The State is responsible for inspecting/upholding a TC lab to CLIA regulations, every two years. Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz at orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: Andy B via Histonet [histonet at lists.utsouthwestern.edu] Sent: Monday, October 05, 2015 12:47 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] TC labs Thank you. That is exactly what I would have given as an answer, but I have yet to see any CLIA inspector or CLIA Operations manual that mentions any of that, other than the vague statements about the Lab Director ultimately being responsible. CLIA is not consistent with its rules and regulations and interpretations. I would love for someone to prove me wrong! When is the last time someone actually witnessed CLIA verifying a TC lab other than maybe being shown some QC logs, etc? I have yet to see any Testing Personnel verifications from CLIA for TC labs. It is hypocritical for CLIA not to inspect TC- only operations. If there is a standard form or regulation for this, please show me. I have never seen or heard of a PC only lab director being asked for verification of testing personnel qualifications from a TC lab. Thanks in advance for everyone's input. On Mon, Oct 5, 2015 at 3:42 PM, Andy B wrote: > Thank you. That is exactly what I would have given as an answer, but I > have yet to see any CLIA inspector or CLIA Operations manual that mentions > any of that, other than the vague statements about the Lab Director > ultimately being responsible. CLIA is not consistent with its rules and > regulations and interpretations. I would love for someone to prove me wrong! > > When is the last time someone actually witnessed CLIA verifying a TC lab > other than maybe being shown some QC logs, etc? I have yet to see any > Testing Personnel verifications from CLIA for TC labs. It is hypocritical > for CLIA not to inspect TC- only operations. If there is a standard form or > regulation for this, please show me. I have never seen or heard of a PC > only lab director being asked for verification of testing personnel > qualifications from a TC lab. > > Thanks in advance for everyone's input. > > > > On Mon, Oct 5, 2015 at 3:28 PM, Morken, Timothy > wrote: > >> What I believe happens is that the referring lab must do an >> audit/inspection of the technical-only lab to be sure they are CLIA >> compliant. For instance, check validation procedures for stains, equipment, >> quality control. It's true CLIA itself does not inspect the Tech-only lab, >> but the referring lab becomes the "technical supervisor" and must have >> documentation to show they have inspected it and it is compliant. That is >> how it works for vendors using other labs to do work for them under FDA >> regulations. >> >> >> Tim Morken >> Pathology Site Manager, Parnassus >> Supervisor, Electron Microscopy/Neuromuscular Special Studies >> Department of Pathology >> UC San Francisco Medical Center >> >> >> >> -----Original Message----- >> From: Andy B via Histonet [mailto:histonet at lists.utsouthwestern.edu] >> Sent: Monday, October 05, 2015 12:05 PM >> To: histonet at lists.utsouthwestern.edu >> Subject: [Histonet] TC labs >> >> Does CLIA inspect labs that only perform Technical Components on tissue? >> >> I know that CAP does so as part of its due diligence, however years ago a >> CLIA inspector told me that CLIA doesnt inspect TC-only operations because >> there is no report (PC) being rendered. How does this jive with grossing >> and the new High Complexity Testing (HCT) paradigm? >> >> In other words, what is to prevent a pathologist (or non-pathologist) >> from owning and operating a TC lab service that is remote from the PC >> service location? How does CMS verify that TC labs are correctly and >> compliantly performing grossing (HCT) and other TC lab evolutions if they >> do not inspect such operations? >> >> I know of labs that are TC only and bill CMS for the Technical Components. >> Has anyone else ever wondered about this? How is CMS assuring that TC >> labs are compliantly staffed and managed if CMS only inspects labs that >> issue reports? >> >> Does anyone wonder how CMS can get away with hammering some labs and >> ignoring others? >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From certndx at gmail.com Mon Oct 5 16:22:52 2015 From: certndx at gmail.com (Cert NDx) Date: Mon, 5 Oct 2015 17:22:52 -0400 Subject: [Histonet] TC labs In-Reply-To: <41400FFE517878449D89114DD2526090196602D283@tocmail1.tocad.orclinic.com> References: <761E2B5697F795489C8710BCC72141FF60319FA1@ex07.net.ucsf.edu> <41400FFE517878449D89114DD2526090196602D283@tocmail1.tocad.orclinic.com> Message-ID: In Oregon perhaps, but not in all states. On Oct 5, 2015 4:52 PM, "Kienitz, Kari" wrote: > The State is responsible for inspecting/upholding a TC lab to CLIA > regulations, every two years. > > > Kari Kienitz HT, (ASCP) > Histology Laboratory > Gastroenterology-EAST > The Oregon Clinic > 1111 NE 99th Ave > Portland, OR 97220 > 503.935.8311 > kkienitz at orclinic.com > > > > > CONFIDENTIALITY WARNING: This e-mail and any attachments are for the > exclusive and confidential use of the intended recipient. If you are not > the intended recipient, please do not read, distribute or take action in > reliance upon this missive. If you have received this in error, please > notify the sender immediately by reply e-mail and delete this message and > its attachments from your computer system. Thank you > ________________________________________ > From: Andy B via Histonet [histonet at lists.utsouthwestern.edu] > Sent: Monday, October 05, 2015 12:47 PM > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] TC labs > > Thank you. That is exactly what I would have given as an answer, but I have > yet to see any CLIA inspector or CLIA Operations manual that mentions any > of that, other than the vague statements about the Lab Director ultimately > being responsible. CLIA is not consistent with its rules and regulations > and interpretations. I would love for someone to prove me wrong! > > When is the last time someone actually witnessed CLIA verifying a TC lab > other than maybe being shown some QC logs, etc? I have yet to see any > Testing Personnel verifications from CLIA for TC labs. It is hypocritical > for CLIA not to inspect TC- only operations. If there is a standard form or > regulation for this, please show me. I have never seen or heard of a PC > only lab director being asked for verification of testing personnel > qualifications from a TC lab. > > Thanks in advance for everyone's input. > > On Mon, Oct 5, 2015 at 3:42 PM, Andy B wrote: > > > Thank you. That is exactly what I would have given as an answer, but I > > have yet to see any CLIA inspector or CLIA Operations manual that > mentions > > any of that, other than the vague statements about the Lab Director > > ultimately being responsible. CLIA is not consistent with its rules and > > regulations and interpretations. I would love for someone to prove me > wrong! > > > > When is the last time someone actually witnessed CLIA verifying a TC lab > > other than maybe being shown some QC logs, etc? I have yet to see any > > Testing Personnel verifications from CLIA for TC labs. It is hypocritical > > for CLIA not to inspect TC- only operations. If there is a standard form > or > > regulation for this, please show me. I have never seen or heard of a PC > > only lab director being asked for verification of testing personnel > > qualifications from a TC lab. > > > > Thanks in advance for everyone's input. > > > > > > > > On Mon, Oct 5, 2015 at 3:28 PM, Morken, Timothy > > > wrote: > > > >> What I believe happens is that the referring lab must do an > >> audit/inspection of the technical-only lab to be sure they are CLIA > >> compliant. For instance, check validation procedures for stains, > equipment, > >> quality control. It's true CLIA itself does not inspect the Tech-only > lab, > >> but the referring lab becomes the "technical supervisor" and must have > >> documentation to show they have inspected it and it is compliant. That > is > >> how it works for vendors using other labs to do work for them under FDA > >> regulations. > >> > >> > >> Tim Morken > >> Pathology Site Manager, Parnassus > >> Supervisor, Electron Microscopy/Neuromuscular Special Studies > >> Department of Pathology > >> UC San Francisco Medical Center > >> > >> > >> > >> -----Original Message----- > >> From: Andy B via Histonet [mailto:histonet at lists.utsouthwestern.edu] > >> Sent: Monday, October 05, 2015 12:05 PM > >> To: histonet at lists.utsouthwestern.edu > >> Subject: [Histonet] TC labs > >> > >> Does CLIA inspect labs that only perform Technical Components on tissue? > >> > >> I know that CAP does so as part of its due diligence, however years ago > a > >> CLIA inspector told me that CLIA doesnt inspect TC-only operations > because > >> there is no report (PC) being rendered. How does this jive with grossing > >> and the new High Complexity Testing (HCT) paradigm? > >> > >> In other words, what is to prevent a pathologist (or non-pathologist) > >> from owning and operating a TC lab service that is remote from the PC > >> service location? How does CMS verify that TC labs are correctly and > >> compliantly performing grossing (HCT) and other TC lab evolutions if > they > >> do not inspect such operations? > >> > >> I know of labs that are TC only and bill CMS for the Technical > Components. > >> Has anyone else ever wondered about this? How is CMS assuring that TC > >> labs are compliantly staffed and managed if CMS only inspects labs that > >> issue reports? > >> > >> Does anyone wonder how CMS can get away with hammering some labs and > >> ignoring others? > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From owensc500 at gmail.com Mon Oct 5 16:50:10 2015 From: owensc500 at gmail.com (Clarence Owens) Date: Mon, 5 Oct 2015 17:50:10 -0400 Subject: [Histonet] Small cell lung blocks Message-ID: <51DBA0A2-E28D-4E20-9F95-D83F52AFF176@gmail.com> Hello all, Does anyone have any small cell lung blocks they can spare for control tissue. I'm working on a new lung antibody and could use some control material. Thanks in advance for your help. Regards Clarence Sent from my iPhone From greg.dobbin at gmail.com Tue Oct 6 07:20:51 2015 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Tue, 6 Oct 2015 09:20:51 -0300 Subject: [Histonet] Looking for fine mesh cassette Message-ID: Hi Folks, I am having difficulty finding a fine mesh biopsy cassette that is compatible with my TBS cassette printer (i.e. *45 degree angle* print surface). The holes in the TBS cassette that I am currently using are too big (~1mm). Can anyone suggest a supplier with a good quality cassette that will work with my printer?? Thank you, Greg Dobbin Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 *Everything in moderation...even moderation itself**!* From leila.etemadi at med.lu.se Tue Oct 6 07:38:43 2015 From: leila.etemadi at med.lu.se (Leila Etemadi) Date: Tue, 6 Oct 2015 12:38:43 +0000 Subject: [Histonet] N/OFQ Message-ID: Hi Histoland, I am planing to use *Anti-Nociception antibody* in a normal IHC procedure on the cryo-sectioned frozen rat brain to detect N/OFQ ( no tricky business such as injecting advance into the brain, developing special C1 or C5 etc. ?) Unfortunately, as I am looking more after a reference, I am finding less? I start to think, if this antibody is exist in the market how is it that no one used it through regular IHC steps, as all other anti bodies? I was wondering if any of you dear specialists out there has any tips or advice for me?!?!, If any one else came cross such a question??!?!?! Your valuable advices is gratefully appreciated in advance! Have a lovely autumn day. Cheers, Leila :-) From Joyce.Weems at emoryhealthcare.org Tue Oct 6 11:19:38 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Tue, 6 Oct 2015 16:19:38 +0000 Subject: [Histonet] Histo control Message-ID: Would anyone happen to have oodles and oodles of Histoplasmosis control tissue you could share with me? I would be very grateful. Thanks!! j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From karla at dermatopathologynorthwest.com Tue Oct 6 16:15:28 2015 From: karla at dermatopathologynorthwest.com (Karla) Date: Tue, 6 Oct 2015 14:15:28 -0700 Subject: [Histonet] what to do with a dead processor Message-ID: <004e01d1007c$21278d80$6376a880$@com> Hello All, I have a very old Leica 1050 tissue processor that is no longer running (bad motherboard) and need the space that it is currently occupying. Any ideas who might want this or how to get rid of it? I have contacted IMEB.and they were not interested (which I fear may be the case for all used equipment vendors). Also of concern is the fact that it has solidified paraffin (contaminated with xylene) in the last 3 stations which I cannot seem to remove. Any suggestions as to how to remove the paraffin without any power to the processor oven? Karla Carlmas Dermatopathology NW PLLC 2330 130th ave NE ste 201 Bellevue, WA 98005 425-455-9945 From jaylundgren at gmail.com Tue Oct 6 20:30:51 2015 From: jaylundgren at gmail.com (Jay Lundgren) Date: Tue, 6 Oct 2015 20:30:51 -0500 Subject: [Histonet] what to do with a dead processor In-Reply-To: <004e01d1007c$21278d80$6376a880$@com> References: <004e01d1007c$21278d80$6376a880$@com> Message-ID: IIRC, don't those paraffin reservoirs slide out? Maybe you could warm them up with a propane torch to get them out? Then put them in an oven or just use brute force and patience to chip the paraffin out? As to who would want it, I don't know? Is a replacement motherboard available? Seems like someone in the 3rd world would be tickled to have it, but who wants to pay to fix it and ship it? :( Jay A. Lundgren, M.S., HTL (ASCP) On Tue, Oct 6, 2015 at 4:15 PM, Karla via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello All, > > > > I have a very old Leica 1050 tissue processor that is no longer running > (bad > motherboard) and need the space that it is currently occupying. Any ideas > who might want this or how to get rid of it? I have contacted IMEB.and they > were not interested (which I fear may be the case for all used equipment > vendors). Also of concern is the fact that it has solidified paraffin > (contaminated with xylene) in the last 3 stations which I cannot seem to > remove. Any suggestions as to how to remove the paraffin without any power > to the processor oven? > > > > Karla Carlmas > > Dermatopathology NW PLLC > > 2330 130th ave NE ste 201 > > Bellevue, WA 98005 > > 425-455-9945 > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From GoetzF at si.edu Wed Oct 7 07:58:04 2015 From: GoetzF at si.edu (Goetz, Freya E.) Date: Wed, 7 Oct 2015 12:58:04 +0000 Subject: [Histonet] Microtome suggestions Message-ID: <4D896FB6-148F-442A-8B0D-2DC4787E3310@si.edu> Good morning! I was wondering if anyone has microtome opinions they would like to share. We have an ultramicrotome but I am looking for a microtome that could cut larger pieces of tissue embedded in plastic. I am hesitant to go with Leica because service is so expensive but I?m looking for honest opinions. Thanks and have a nice day! Freya From Valerie.Hannen at parrishmed.com Wed Oct 7 09:39:25 2015 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Wed, 7 Oct 2015 10:39:25 -0400 Subject: [Histonet] Labeling formats In-Reply-To: References: <92B59D6A-774A-428C-AD5F-A3B458CEF7D8@4path.com> <450B7A81EDA0C54E97C53D60F00776C32347EC18E1@isexstore03> Message-ID: <450B7A81EDA0C54E97C53D60F00776C32347EC18E6@isexstore03> Thank you so much for the article Sue. After reading the article, I have determined that I will NOT be changing our labeling format (yr-prefix-accession number-part (alpha)-block number, as the article stated that there was inadequate evidence to support a specific labeling format. I however will be adding the 2nd identifier to our blocks and to the hand-written slides (prior to application of paper label, which has all the required information) beginning in 2016. If we have a specimen that has been prepared (ex. FNA slides) by our personnel away from the accessioning area, we already include the 2 identifiers on the slides. We also already add the special stain name to the slides and labels. Thanks again for the enlightenment. Valerie Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Breckenridge, Sue [mailto:sbreckenridge at caperegional.com] Sent: Tuesday, October 06, 2015 12:13 PM To: 'Stephen G. Ruby' Cc: Hannen, Valerie Subject: RE: [Histonet] Labeling formats See attached -----Original Message----- From: Stephen G. Ruby [mailto:Sruby at 4path.com] Sent: Tuesday, October 06, 2015 12:09 PM To: Breckenridge, Sue; 'Hannen, Valerie' Subject: RE: [Histonet] Labeling formats Does anyone have a pre-publication release of that? Or at least the recommendations ? Stephen G. Ruby, MD, MBA, FCAP President and Medical Director, 4path, Ltd. Value, Service and Commitment...Beyond the Diagnosis V: 1-877-884-7284 F: 630-780-4909 www.4path.com 8238 S. Madison St. Burr Ridge, IL 60527 THIS ELECTRONIC MAIL TRANSMISSION AND ANY ATTACHMENTS MAY CONTAIN PRIVILEGED, CONFIDENTIAL, OR PROPRIETARY INFORMATION INTENDED ONLY FOR THE PERSON(S) NAMED. IF THE READER OF THIS MESSAGE IS NOT THE INTENDED RECIPIENT OR THE AUTHORIZED REPRESENTATIVE OF THE INTENDED RECIPIENT, YOU ARE HEREBY NOTIFIED THAT ANY DISTRIBUTION, COPYING, OR DISCLOSURE OF THIS COMMUNICATION IS STRICTLY PROHIBITED. Pursuant to the above rights and privileges, immediately DELETE and DESTROY all copies of the email and its attachments, in whatever form, and immediately NOTIFY the sender of your receipt of this email. DO NOT review, copy, or rely on in any way the contents of this email and its attachments. All rights of the sender for violations of the confidentiality and privileges applicable to this email and any attachments are expressly reserved. This E-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. Sections 2510-2521, is confidential and may be legally privileged. -----Original Message----- From: Breckenridge, Sue [mailto:sbreckenridge at caperegional.com] Sent: Tuesday, October 06, 2015 10:12 AM To: 'Hannen, Valerie'; Stephen G. Ruby Subject: RE: [Histonet] Labeling formats We use same as Valerie's here, but will change January 1, 2016 to the S-16-1234A-1 format to be in line with early released article "Uniform Labeling of Blocks and Slides in Surgical Pathology", a joint guideline from the CAP, NSH and Laboratory Quality Center. (Archives of Pathology and Lab Medicine special article accepted for publication 3-12-15). Sue ________________________________________ Susan Breckenridge, BS, HT/HTL(ASCP) Histology Supervisor Cape Regional Medical Center 2 Stone Harbor Blvd Cape May Court House, NJ 08210 Phone: 609-463-2449 Fax: 609-463-2747 sbreckenridge at caperegional.com ________________________________________ -----Original Message----- From: Hannen, Valerie via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, October 02, 2015 10:56 AM To: 'Stephen G. Ruby' Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Labeling formats Ours is very similar...only difference being ours is: 15S-1234A-1 (year-prefix-accession number-part(alpha)-block number(numeric). Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com http://cp.mcafee.com/d/avndy0Qd3gsrhooohoopjhdTdFTuhv7fCzAsedFTuhv7enshdFTuhv7fCzAsqejqtTAnAmmmrCWhr85wkMJIOvbCS5JCjVsSzpzSkn7-LP3dXFKtuVtddW_ffccc6zDBHEShhlKO_OEuvkzaT0QSyrpdTWXb2aaqrXP1KVIFOSNzaxdnQendQ8h-NnUhAwhY5mRrU-hBm6DYKrs7nvdwIqid41Fr2YGjG3jiWq80HF3PVg8Cy1SIltDgSOyr8Q_MM49O -----Original Message----- From: Stephen G. Ruby via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, October 02, 2015 10:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Labeling formats I am curious on what formats that labs use for labeling. We use prefix-year-accession number-part(alpha)-block number(numeric) Example. S15-12345-A1 If you use a different format can you share it here? Thanks. Dr Ruby Sent from my mobile device _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://cp.mcafee.com/d/avndz8s91NJ5xxx5xxBd4TsSDtV5Ys-qehMUSDtV5YsVtN4SDtV5Ys-qehNEVdFTuhuhpppKrF5Iwm1j2SP9YKromSpfBPqdCfphsvW_ccTKCVRXBQQTHYYYMMMqeumKzp55mXb_axVZicHs3jqpJATvHII8EFFLLc6XCM0pYGjFPsWlrCjcvivNxTEdU9GX33VkDa3Js9OFeEddQ8h-NnUhAwhY5mRrU-hBm6DYKrs7nvdwIqid41Fr2YGjG3jiWq80HF3PVg8Cy1SIltDgSOyrqVqu-It4 ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://cp.mcafee.com/d/1jWVIp3xESyMMMyMMOCyrKrjKYy-evd78UsrjKYy-esKUyrjKYy-evd78UQsCQXL8L8IIITdQySgb0FxrpA-ndIbrcDOVJ6P7IEKfZvC6rTjsWZOWqrR-uuoood7fbnhIyyHtB_BgY-F6lK1FJASOrLRSm4kkQTTC3tPo0c-l9QVKtaJP9CfFfUMXQ6Y4RtxxYGjB1SK4VkDk6CW48_oHY8Og8-2HqJYv8OH3j-ndK3HLCMmd96y0QJxul9R1FFtd40lQxVYE4jh0XmaKPErphdSk8fu2wJ91_d Confidentiality Notice: This e-mail message, including any attachments, from Cape Regional Health System contains information which is CONFIDENTIAL AND/OR LEGALLY PRIVILEGED.The information is intended only for the use of the individual named above and may not be disseminated to any other party without Cape Regional Health System's written permission. If you are not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this e-mailed information is strictly prohibited. If you have received this e-mail in error, please notify us immediately by telephone at 609-463-2163 to arrange for the return of these documents to us without cost to you. Confidentiality Notice: This e-mail message, including any attachments, from Cape Regional Health System contains information which is CONFIDENTIAL AND/OR LEGALLY PRIVILEGED.The information is intended only for the use of the individual named above and may not be disseminated to any other party without Cape Regional Health System's written permission. If you are not the intended recipient, or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this e-mailed information is strictly prohibited. If you have received this e-mail in error, please notify us immediately by telephone at 609-463-2163 to arrange for the return of these documents to us without cost to you. ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From rjbuesa at yahoo.com Wed Oct 7 10:58:59 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 7 Oct 2015 15:58:59 +0000 (UTC) Subject: [Histonet] Microtome suggestions In-Reply-To: <4D896FB6-148F-442A-8B0D-2DC4787E3310@si.edu> References: <4D896FB6-148F-442A-8B0D-2DC4787E3310@si.edu> Message-ID: <1596232501.225804.1444233539457.JavaMail.yahoo@mail.yahoo.com> For large specimens you need a "horizontal" or sledge microtome. Leitz (Leica) manufactures the best, but you could try an OMS from Reichert although I am not sure they stll are manufactured because Leica swallowed Reichert some years ago.Another alternative, and probably even better than the Leitz (Leica), is a Spencer (American Optical) sliding microtome Model 860 For me this is the best all-around ever manufactured sliding microtome and not too long ago a saw one for sale in e-Bay. Why don't you try looking for it. It will be much cheaper than any modern horizontal microtome and far better than any new one.Ren? J. On Wednesday, October 7, 2015 9:08 AM, "Goetz, Freya E. via Histonet" wrote: Good morning! I was wondering if anyone has microtome opinions they would like to share. We have an ultramicrotome but I am looking for a microtome that could cut larger pieces of tissue embedded in plastic. I am hesitant to go with Leica because service is so expensive but I?m looking for honest opinions. Thanks and have a nice day! Freya _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abadesuyi at nrh-ok.com Wed Oct 7 11:03:48 2015 From: abadesuyi at nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Wed, 7 Oct 2015 11:03:48 -0500 Subject: [Histonet] Tissue Sample Quality - ANP.10038 Message-ID: <04EE4F75BB5FB246ADB68D69B7460443A405DD9FBC@MAIL.nrhnt.nrh-ok.com> Hi, I hope you guys are doing great. I am about to write Tissue Sample Quality procedure, the new CAP Checklist ANP.10038 and I was wondering if I could get an input from you guys. ANP.10038 Tissue Sample Quality There is a procedure that describes the process by which histotechnologists provide feedback to submitting pathologists and pathology assistants on the quality of the gross tissue sections received for tissue processing. Records of such feedback and corrective action taken when problems are identified may be incorporated into the laboratory's quality management program. Thanks, Banjo Adesuyi ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From GoetzF at si.edu Wed Oct 7 11:07:23 2015 From: GoetzF at si.edu (Goetz, Freya E.) Date: Wed, 7 Oct 2015 16:07:23 +0000 Subject: [Histonet] Microtome suggestions In-Reply-To: <1596232501.225804.1444233539457.JavaMail.yahoo@mail.yahoo.com> References: <4D896FB6-148F-442A-8B0D-2DC4787E3310@si.edu> <1596232501.225804.1444233539457.JavaMail.yahoo@mail.yahoo.com> Message-ID: <0274D928-6107-4F40-8406-645E95965BEF@si.edu> Hi Rene and histonet, Thank you for your suggestions. I must amend my earlier request. We have an ultra microtome from RMC and a Spencer 820 microtome. I would like to section epoxy embedded material that is larger than an ultramicrotome could handle but smaller than a sledge microtome. Sorry I wasn?t specific. Thank you again! Freya On Oct 7, 2015, at 12:01 PM, Rene J Buesa > wrote: For large specimens you need a "horizontal" or sledge microtome. Leitz (Leica) manufactures the best, but you could try an OMS from Reichert although I am not sure they stll are manufactured because Leica swallowed Reichert some years ago. Another alternative, and probably even better than the Leitz (Leica), is a Spencer (American Optical) sliding microtome Model 860 For me this is the best all-around ever manufactured sliding microtome and not too long ago a saw one for sale in e-Bay. Why don't you try looking for it. It will be much cheaper than any modern horizontal microtome and far better than any new one. Ren? J. On Wednesday, October 7, 2015 9:08 AM, "Goetz, Freya E. via Histonet" > wrote: Good morning! I was wondering if anyone has microtome opinions they would like to share. We have an ultramicrotome but I am looking for a microtome that could cut larger pieces of tissue embedded in plastic. I am hesitant to go with Leica because service is so expensive but I?m looking for honest opinions. Thanks and have a nice day! Freya _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann at aol.com Wed Oct 7 12:24:29 2015 From: thisisann at aol.com (thisisann at aol.com) Date: Wed, 7 Oct 2015 13:24:29 -0400 Subject: [Histonet] Expiration date for Special Stain Control slides Message-ID: <15043553694-19c7-2afa4@webprd-a94.mail.aol.com> Does anyone know if expiration dates from purchased control slides are necessary. Is there a regulation that requires expiration dates on purchased controls? We purchase from two different companies, one vendor has the expiration date on the box and one does not include it. Any help would be appreciated. Thanks, Ann From KSimeone at leavittmgt.com Wed Oct 7 12:35:31 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Wed, 7 Oct 2015 17:35:31 +0000 Subject: [Histonet] FULLTIME DAY SHIFT (first shift) AND FULL TIME NIGHT SHIFT (IHC) POSITION DELRAY BCH FL In-Reply-To: <43944B1DBAAC2846B7B9D626B5F1233C4D2DDDED@vm-email.leavittmgt.com> References: <43944B1DBAAC2846B7B9D626B5F1233C4D2DDB61@vm-email.leavittmgt.com>, <43944B1DBAAC2846B7B9D626B5F1233C4D2DDDED@vm-email.leavittmgt.com> Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C4D2DE034@vm-email.leavittmgt.com> Hi Histonetters! We are looking for TWO full time licensed histotechs here in our very busy Delray Beach, Florida dermatology laboratory. These are permanent full time, (40 hours) position with benefits (medical/401k/vacation). Annual salary in the $50k range (NIGHT SHIFT DIFF AVAILABLE). THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! DAYTIME POSTION: *full time position Mon-Fri 8a-5:30p (start time may vary) *MUST be licensed as a FLORIDA HISTOTECHNICIAN OR HISTOTECHNOLOGIST (NO pending licensure pls) *PREFER experience but WILL TRAIN a knowledgeable, willing candidate *duties to include (but not limited to): grossing, microtomy, accessioning, embedding and general histology *must be self motivated, reliable and a team player DAYTIME SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY: https://advancedderm.applicantpro.com/jobs/240090.html NIGHT POSITION: *Full time position Mon-Fri 10:30p-7:00a (IMMUNOHISTOCHEMISTRY/SPECIALS department) *MUST be licensed as a FLORIDA HISTOTECHNOLOGIST (this is NOT negotiable) *EXPERIENCE WITH IHC A MUST! Leica (BOND) and Roche/Ventana equipment experience preferred *Must be able to multi-task special stains *MUST have at LEAST 2 years experience. Please DO NOT respond if you do not possess EXPERIENCE in this area! *must be confidant, quick learner, self motivated, reliable and a team player NIGHT SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY: https://advancedderm.applicantpro.com/jobs/278020.html Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From hhawkins at UTMB.EDU Wed Oct 7 13:05:58 2015 From: hhawkins at UTMB.EDU (Hawkins, Hal K.) Date: Wed, 7 Oct 2015 18:05:58 +0000 Subject: [Histonet] Microtome suggestions In-Reply-To: <0274D928-6107-4F40-8406-645E95965BEF@si.edu> References: <4D896FB6-148F-442A-8B0D-2DC4787E3310@si.edu> <1596232501.225804.1444233539457.JavaMail.yahoo@mail.yahoo.com>, <0274D928-6107-4F40-8406-645E95965BEF@si.edu> Message-ID: <22624908330375439D6382C9F95093FF581E2509@GRMBX1.utmb.edu> The old Sorvall JB-4 microtome was designed for this purpose and works well. They are available at a reasonable price from several used equipment providers and on Ebay. ________________________________________ From: Goetz, Freya E. via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, October 07, 2015 11:07 AM To: Rene J Buesa Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome suggestions Hi Rene and histonet, Thank you for your suggestions. I must amend my earlier request. We have an ultra microtome from RMC and a Spencer 820 microtome. I would like to section epoxy embedded material that is larger than an ultramicrotome could handle but smaller than a sledge microtome. Sorry I wasn?t specific. Thank you again! Freya On Oct 7, 2015, at 12:01 PM, Rene J Buesa > wrote: For large specimens you need a "horizontal" or sledge microtome. Leitz (Leica) manufactures the best, but you could try an OMS from Reichert although I am not sure they stll are manufactured because Leica swallowed Reichert some years ago. Another alternative, and probably even better than the Leitz (Leica), is a Spencer (American Optical) sliding microtome Model 860 For me this is the best all-around ever manufactured sliding microtome and not too long ago a saw one for sale in e-Bay. Why don't you try looking for it. It will be much cheaper than any modern horizontal microtome and far better than any new one. Ren? J. On Wednesday, October 7, 2015 9:08 AM, "Goetz, Freya E. via Histonet" > wrote: Good morning! I was wondering if anyone has microtome opinions they would like to share. We have an ultramicrotome but I am looking for a microtome that could cut larger pieces of tissue embedded in plastic. I am hesitant to go with Leica because service is so expensive but I?m looking for honest opinions. Thanks and have a nice day! Freya _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Wed Oct 7 14:49:16 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 7 Oct 2015 19:49:16 +0000 Subject: [Histonet] Tissue Sample Quality - ANP.10038 In-Reply-To: <04EE4F75BB5FB246ADB68D69B7460443A405DD9FBC@MAIL.nrhnt.nrh-ok.com> References: <04EE4F75BB5FB246ADB68D69B7460443A405DD9FBC@MAIL.nrhnt.nrh-ok.com> Message-ID: <761E2B5697F795489C8710BCC72141FF6031B180@ex07.net.ucsf.edu> Banjo, We use a form to inform pathologists/residents of tissue problems. Our SOP is that anything that is noticed at embedding or sectioning that either compromises quality or prevents proceeding is noted on the form and sent along to the pathologist/resident. If we cannot proceed due to quality problems we notify the pathologist/resident immediately (tho if it is 3AM we send an email and then page them around 8AM). The form lists the common problems. It is printed on bright yellow paper to stand out from the other paperwork. It has a border consisting of pictures of a hexagonal STOP sign to stand out. The info is also entered in our LIS system as a QA flag for that specimen. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Adesupo, Adesuyi (Banjo) via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, October 07, 2015 9:04 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Tissue Sample Quality - ANP.10038 Hi, I hope you guys are doing great. I am about to write Tissue Sample Quality procedure, the new CAP Checklist ANP.10038 and I was wondering if I could get an input from you guys. ANP.10038 Tissue Sample Quality There is a procedure that describes the process by which histotechnologists provide feedback to submitting pathologists and pathology assistants on the quality of the gross tissue sections received for tissue processing. Records of such feedback and corrective action taken when problems are identified may be incorporated into the laboratory's quality management program. Thanks, Banjo Adesuyi ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From flor.m.olivares at gmail.com Wed Oct 7 17:07:23 2015 From: flor.m.olivares at gmail.com (Flor Olivares) Date: Wed, 7 Oct 2015 18:07:23 -0400 Subject: [Histonet] Histonet] Histology Internship in Gaithersburg Md available Message-ID: <84D469FE-E407-435B-B67F-0B89D11F1777@gmail.com> Good afternoon, I'm Flor Olivares, currently a Montgomery College student. I have plans to begin the histology technician online program at Harford Community College. Therefore, I need to have a site where I can do my laboratory practice. I just have an introductory knowledge of chemistry. I would love to be considered for this internship. I can be contacted at flor.m.olivares at gmail.com Have a good evening. Thank you, Flor Olivares http://www.histosearch.com/histonet/Mar08A/HistonetHistologyInternsh.html Sent from my iPhone From turkekul at gmail.com Wed Oct 7 17:36:25 2015 From: turkekul at gmail.com (Mesru T) Date: Wed, 7 Oct 2015 18:36:25 -0400 Subject: [Histonet] Histonet Digest, Vol 143, Issue 3 In-Reply-To: References: Message-ID: Hello Histonetters, I have read in the past e-mails that some of you have used CD8a Rat monoclonal from Angio-Proteomie cat#mAP-0036 for IHC on FFPE mouse tissue. I have tried the antibody on Lieca Bond RX and did not get any good result. Could you share your protocol? My protocol using Bond Polymer Refine kit was: Dewax ER2 20min Peroxidase block 5min Marker (CD*8 1:100) Rabbit anti-rat 8min Polymer 8min DAB 10min Hematoxylin 10min Any help is appreciated. Thanks, Mesru > > > > > > > > > > > > > > > > > > > > > > From cdemarinis at SARATOGACARE.ORG Thu Oct 8 06:52:44 2015 From: cdemarinis at SARATOGACARE.ORG (Demarinis, Carolyn) Date: Thu, 8 Oct 2015 11:52:44 +0000 Subject: [Histonet] Fatty breast tissue Message-ID: <1C75A843982A7B44BB368A3CC946ABCA012D591DD4@SHEXCHMBX01.SARAHOSP.ORG> What are the best choices for processing fatty breast tissue when the pathologist grosses the tissue the same day it is excised? -- This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy at saratogacare.org and destroy all copies of this communication and any attachments. From foreightl at gmail.com Thu Oct 8 09:04:22 2015 From: foreightl at gmail.com (Patrick Laurie) Date: Thu, 8 Oct 2015 10:04:22 -0400 Subject: [Histonet] Fatty breast tissue In-Reply-To: <1C75A843982A7B44BB368A3CC946ABCA012D591DD4@SHEXCHMBX01.SARAHOSP.ORG> References: <1C75A843982A7B44BB368A3CC946ABCA012D591DD4@SHEXCHMBX01.SARAHOSP.ORG> Message-ID: The key is to make sure that you have enough formalin fixation (greater than 6 hours for ASCO/CAP standards for biomarker standards). That is a bare minimum, and if the sections are thick, usually unacceptable cutting quality will result. It would be best if the pathologist could wait at least 12 or more hours, it will result in better morphology. If the pathologist insists on processing the same day, with minimum fixation, perhaps set up a protocol on your processor that includes a couple of hours of fixation (with pressure, can help) to add on. More time in the dehydration steps and clearing steps will also help. Remember, though, when you calculate the formalin fixation times, you need to add the time that it was on the processor too. Good luck Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Thu, Oct 8, 2015 at 7:52 AM, Demarinis, Carolyn via Histonet < histonet at lists.utsouthwestern.edu> wrote: > What are the best choices for processing fatty breast tissue when the > pathologist grosses the tissue the same day it is excised? > > > -- > > > This e-mail communication and any attachments may contain > confidential and privileged information for the use of the > designated recipients named above. > If you are not the intended recipient, you are hereby notified > that you have received this communication in error and that > any review, disclosure, dissemination, distribution or copying > of it or its contents is prohibited. If you have received this > communication in error, please notify Saratoga Hospital > immediately by e-mail at privacy at saratogacare.org and > destroy all copies of this communication and any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rsrichmond at gmail.com Thu Oct 8 15:26:02 2015 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 8 Oct 2015 16:26:02 -0400 Subject: [Histonet] Fatty breast tissue Message-ID: Carolyn Demarinis at Saratoga Springs NY asks: >>What are the best choices for processing fatty breast tissue when the pathologist grosses the tissue the same day it is excised?<< Breast tissue should always be grossed promptly, and placed in cassettes on the day it's excised - that's particularly important with open biopsy material such as wire localization specimens. Those cassettes should then sit in formalin overnight, and get processed the next day. You simply cannot process fatty breast tissue with insufficient fixation - you don't get good sections, and you compromise the ER/PR and HER2 immunostains. Even needle biopsy specimens that are obtained late in the day need overnight fixation. Overnight fixation is particularly important if the pathologist is given to cramming cassettes with fatty tissue, as so many of us do. Pathologists sometimes need to remind surgeons that, while you understand the patient's anxiety, that the patient needs a right diagnosis, not a quick diagnosis. Surgeons need to tell breast surgery patients before surgery that while the lab is fast, that inevitably some diagnoses will be delayed. I've been grossing breast tissue for 50 years, and I've made most of the grossing mistakes it's possible to make. Bob Richmond Samurai Pathologist Maryville TN From patpxs at gmail.com Thu Oct 8 18:25:00 2015 From: patpxs at gmail.com (P Sicurello) Date: Thu, 8 Oct 2015 16:25:00 -0700 Subject: [Histonet] Elastic Stain on Ventana BenchMark Special Stainer Message-ID: Hello Histoes, I've got a Ventana BenchMark Special Stainer and the best I am getting so far is purple. Does anyone have a protocol they have worked out that makes the elastic fibers black? If you have a protocol will you share it with me please? Thank you, Paula UCSanDiego Health System From e.kooijman at vumc.nl Fri Oct 9 07:46:24 2015 From: e.kooijman at vumc.nl (Kooijman, Esther) Date: Fri, 9 Oct 2015 12:46:24 +0000 Subject: [Histonet] autoradiography frozen human brain sections fall off, white matter issue, solved !!! Message-ID: <51AEA3C85E64ED4E851909E3CCAB574CC9A94ACB@SP-MX-MBX2.vumc.nl> Dear Histonetters, Thank you Barbara, Wendy and Brett and others that were so kind to give me hints and tips ! I managed to solve the problem, all tissue stays on. I have used Superfrost Plus Gold. And a absorbent pouch inside the storage case. After cutting keeping them melting on longer about an hour at room temp. Then again like we used to in silica in the fridge ON. When getting them out leaving them in the box for an hour to defrost. And........ all the tissue stays on during the complete procedure !!! @Barbara no need to try the fixing step ;-) Thank you all for your input ! Have a nice weekend. Esther -----Oorspronkelijk bericht----- Van: Connolly, Brett M [mailto:brett_connolly at merck.com] Verzonden: vrijdag 2 oktober 2015 16:08 Aan: Kooijman, Esther CC: histonet at lists.utsouthwestern.edu Onderwerp: RE: [Histonet] autoradiography frozen human brain sections fall off, white matter issue Hi Esther, We do a lot of autoradiography on rodent, primate and human brains sections. Our protocol is similar to yours except we do not dry the slides after incubating in the buffer and before adding the radiotracer. The experiment is performed with the slides in a rack and totally immersed in the solutions in staining dishes. 1. incubate slides in assay buffer 15 min. at RT 2. add radiotracer to buffer, gently mix and incubate 90-120 min. at RT 3. wash GENTLY (no agitation) in ice-cold wash buffer 3 x 3 min. We set up 3 containers and transfer the slides. 4. wash in ice-cold DI water 5 seconds. 5. air dry The Superfrost gold slides are supposed to improve adherence and would be worth a try. It could be your other Superfrost slides are old or a bad batch...which I think has been experienced by some people on the list. We do store our sectioned slides at -70C until use and then bring them up to RT the day of the experiment. They are sectioned, dried at RT for 15-20 min, and then store at -70C... No drying in the fridge. Good luck, Brett Brett M. Connolly, Ph.D. Principle Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: Kooijman, Esther via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, October 02, 2015 5:20 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] autoradiography frozen human brain sections fall off, white matter issue Importance: High Dear Histonetters, Does anyone of you have experience in autoradiography and could help me with the problem falling of tissue ? Or suggestions ? We have problems with falling off sections of human brain tissue. We have done the same experiments and cutting in rodent tissue without any problems. Our protocol for autoradiography : Snap frozen tissue, cut in cryo 20um sections on Superfrost plus glass, drying ON in the fridge on silica. Stored in -20. Day of the experiment. Getting them on room temperature about 45 minutes, washing/dehydrate on room temperature in 5mM Tris (HCL PH7.4) buffer, 20 minutes. About 30 minutes - an hour drying at room temperature. Then incubation with the radio tracer for 30 minutes, room temperature, just dripping the 1 mL solution to completely cover the superfrost plus glass and ditto tissue. Turning the glass to get the solution of the glass, dipping in ice cold tris washing buffer 5mM (HCL PH7.4) 1.5 minutes and here the disaster starts with falling off extensive white matter falling off...... its washing steps of only 1.5 minute. What can I do to prevent this, any idea ? I am out of clues. I have tried to dry the sections longer after cutting sections, I have tried to pre wash in either room temp or cold buffer. Hope someone can help me. Would Superfrost plus gold glass be better ? Kind Regards, Esther Kooijman Research Technician VU University Medical Center Nuclear Medicine & PET Research Email: e.kooijman at vumc.nl Amsterdam The Netherlands _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From TanyaAbbott at catholichealth.net Fri Oct 9 08:34:35 2015 From: TanyaAbbott at catholichealth.net (Abbott, Tanya) Date: Fri, 9 Oct 2015 13:34:35 +0000 Subject: [Histonet] Coverslip tape Message-ID: <852F7D2C14FB464D80E182B15DB138AF7B55EA55@CHIEX005.CHI.catholichealth.net> This is more a Cyto question, but, does anyone out there use the Sakura Coverslip tape to coverslip their Thin Preps? We seem to be having a problem down the sides of the slide where the tape is not being pushed down and access glue seems to accumulate there. Sakura tells us it's a drying issue but I really don't think it is, we let the slides dry long enough. It seems to me that the bar that pushes the tape down is not pushing down well enough on the sides. We have changed the springs recently and are on a new roll of tape. Our H&E slides don't seem to be affected. Any input is appreciated! Happy Friday! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology Penn State Health St. Joseph (phone) 610-378-2635 From blayjorge at gmail.com Fri Oct 9 08:57:09 2015 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Fri, 9 Oct 2015 09:57:09 -0400 Subject: [Histonet] Coverslip tape In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF7B55EA55@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF7B55EA55@CHIEX005.CHI.catholichealth.net> Message-ID: Hello: Is there a way to get a small free sample of that tape? Does it save money or have some other pluses? Gratefully, Jorge On Fri, Oct 9, 2015 at 9:34 AM, Abbott, Tanya via Histonet < histonet at lists.utsouthwestern.edu> wrote: > This is more a Cyto question, but, does anyone out there use the Sakura > Coverslip tape to coverslip their Thin Preps? We seem to be having a > problem down the sides of the slide where the tape is not being pushed down > and access glue seems to accumulate there. Sakura tells us it's a drying > issue but I really don't think it is, we let the slides dry long enough. It > seems to me that the bar that pushes the tape down is not pushing down well > enough on the sides. We have changed the springs recently and are on a new > roll of tape. Our H&E slides don't seem to be affected. > Any input is appreciated! Happy Friday! > Tanya > > Tanya G. Abbott > Manager Technologist > Histology/Cytology > Penn State Health St. Joseph > (phone) 610-378-2635 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From amosbrooks at gmail.com Mon Oct 12 06:21:56 2015 From: amosbrooks at gmail.com (Amos Brooks) Date: Mon, 12 Oct 2015 07:21:56 -0400 Subject: [Histonet] Purple elastic In-Reply-To: References: Message-ID: Hi, There is a reason that every manual with an elastic procedure says that the stain should be made up fresh. My guess is that the company is trying to prolong the shelf life by removing or reducing either the iodine or ferric chloride. Doing this will prevent the hematoxylin from turning black. If it don't work on the machine, you should do it by hand. The results will be MUCH better. And this stain is not that difficult. Cheers, Amos From Elise_ODea at URMC.Rochester.edu Mon Oct 12 12:12:38 2015 From: Elise_ODea at URMC.Rochester.edu (ODea, Elise) Date: Mon, 12 Oct 2015 17:12:38 +0000 Subject: [Histonet] Microtomy statisitcs Message-ID: <17c1054e95714ae2ac2a00d8579ab833@exmbxpdc06.urmc-sh.rochester.edu> I imagine there are several schools of thought on this topic, but here goes: How many blocks should a histotech cut for 1 H&E per hour? Other duties during this time are drying, loading and unloading a stainer and coverslipper. Thanks! Elise Elise T. O'Dea, MT, ASCP Histology Supervisor Highland Hospital 1000 South Ave. Rochester, New York 14620 office 585.341.6596 lab 585.341.8314 elise_odea at urmc.rochester.edu Confidentiality Notice This transmission contains confidential information protected by New York State law and HIPAA regulations. You are prohibited from making any further disclosure of this information without the specific written consent of the person to whom it pertains, or as otherwise permitted by law. A general authorization for the release of medical or other information is not sufficient authorization for further disclosure of information, which is protected by New York State Public Health Law Article 27-F or Title 42 of the Code of Federal Regulations. Any unauthorized further disclosure in violation of State law may result in a fine or jail sentence or both. If you have received this material in error, please notify the sender IMMEDIATELY to arrange for the return or destruction of the document(s). From rjbuesa at yahoo.com Mon Oct 12 14:15:02 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 12 Oct 2015 19:15:02 +0000 (UTC) Subject: [Histonet] Microtomy statisitcs In-Reply-To: <17c1054e95714ae2ac2a00d8579ab833@exmbxpdc06.urmc-sh.rochester.edu> References: <17c1054e95714ae2ac2a00d8579ab833@exmbxpdc06.urmc-sh.rochester.edu> Message-ID: <932792051.2671484.1444677302157.JavaMail.yahoo@mail.yahoo.com> 24Ren? J. On Monday, October 12, 2015 1:29 PM, "ODea, Elise via Histonet" wrote: I imagine there are several schools of thought on this topic, but here goes:? How many blocks should a histotech cut for 1 H&E per hour?? Other duties during this time are drying, loading and unloading a stainer and coverslipper. Thanks! Elise Elise T. O'Dea, MT, ASCP Histology Supervisor Highland Hospital 1000 South Ave. Rochester, New York 14620 office 585.341.6596 lab 585.341.8314 elise_odea at urmc.rochester.edu Confidentiality Notice This transmission contains confidential information protected by New York State law and HIPAA regulations. You are prohibited from making any further disclosure of this information without the specific written consent of the person to whom it pertains, or as otherwise permitted by law. A general authorization for the release of medical or other information is not sufficient authorization for further disclosure of information, which is protected by New York State Public Health Law Article 27-F or Title 42 of the Code of Federal Regulations. Any unauthorized further disclosure in violation of State law may result in a fine or jail sentence or both. If you have received this material in error, please notify the sender IMMEDIATELY to arrange for the return or destruction of the document(s). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Mon Oct 12 15:26:24 2015 From: patpxs at gmail.com (P Sicurello) Date: Mon, 12 Oct 2015 13:26:24 -0700 Subject: [Histonet] Microtomy statisitcs In-Reply-To: <932792051.2671484.1444677302157.JavaMail.yahoo@mail.yahoo.com> References: <17c1054e95714ae2ac2a00d8579ab833@exmbxpdc06.urmc-sh.rochester.edu> <932792051.2671484.1444677302157.JavaMail.yahoo@mail.yahoo.com> Message-ID: Rene' has a really good paper that discusses this topic. http://www.histosearch.com/ADP9ProductivityStandards.pdf The larger issues are how to track the number cut per hour. It's easy to get a daily rate, but breaking it down to blocks/hour when techs are doing multiple tasks makes it a bit more challenging. Are people willing to share how they track the number of blocks cut per hour per tech? Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UCSan Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Mon, Oct 12, 2015 at 12:15 PM, Rene J Buesa via Histonet < histonet at lists.utsouthwestern.edu> wrote: > 24Ren? J. > > > On Monday, October 12, 2015 1:29 PM, "ODea, Elise via Histonet" < > histonet at lists.utsouthwestern.edu> wrote: > > > I imagine there are several schools of thought on this topic, but here > goes: How many blocks should a histotech cut for 1 H&E per hour? Other > duties during this time are drying, loading and unloading a stainer and > coverslipper. > > Thanks! > Elise > Elise T. O'Dea, MT, ASCP > Histology Supervisor > Highland Hospital > 1000 South Ave. > Rochester, New York 14620 > office 585.341.6596 > lab 585.341.8314 > elise_odea at urmc.rochester.edu > > > Confidentiality Notice > This transmission contains confidential information protected by New York > State law and HIPAA regulations. You are prohibited from making any further > disclosure of this information without the specific written consent of the > person to whom it pertains, or as otherwise permitted by law. A general > authorization for the release of medical or other information is not > sufficient authorization for further disclosure of information, which is > protected by New York State Public Health Law Article 27-F or Title 42 of > the Code of Federal Regulations. Any unauthorized further disclosure in > violation of State law may result in a fine or jail sentence or both. If > you have received this material in error, please notify the sender > IMMEDIATELY to arrange for the return or destruction of the document(s). > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Lisa.White3 at va.gov Tue Oct 13 12:43:23 2015 From: Lisa.White3 at va.gov (White, Lisa M.) Date: Tue, 13 Oct 2015 13:43:23 -0400 Subject: [Histonet] Microtomy statisitcs Message-ID: <2B2ECF33934F5D4996D8BE03EFDF39760C478854@VHAV09MSGA3.v09.med.va.gov> We have a list target of 30 blocks per hour. However we don't track this. There are so many distractions incoming frozen sections, phone calls, drying, loading/unloading a stainer/coverslipper, loading/unloading the IHC stainer, loading/unloading the special stainer, pathologist with questions, a panic filled clinic clerk runs in and is out of pre-filled formalin cups and the doctor is doing a procedure, the laundry delivery, the shred paper guy doing a pick up......well you get the picture. This is why we feel it is unfair to utilize a number of slides/blocks per hour. If there are multiple HT as there is here then it sets up an atmosphere to where no one will be willing to leave the microtome to perform the other duties. I would rather not have tech's rushing to attain a quota instead of producing quality slides. Every task has to be completed in the end. I guess each lab just picks its battles. -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Tuesday, October 13, 2015 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 143, Issue 10 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Microtomy statisitcs (ODea, Elise) 2. Re: Microtomy statisitcs (Rene J Buesa) 3. Re: Microtomy statisitcs (P Sicurello) ---------------------------------------------------------------------- Message: 1 Date: Mon, 12 Oct 2015 17:12:38 +0000 From: "ODea, Elise" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Microtomy statisitcs Message-ID: <17c1054e95714ae2ac2a00d8579ab833 at exmbxpdc06.urmc-sh.rochester.edu> Content-Type: text/plain; charset="us-ascii" I imagine there are several schools of thought on this topic, but here goes: How many blocks should a histotech cut for 1 H&E per hour? Other duties during this time are drying, loading and unloading a stainer and coverslipper. Thanks! Elise Elise T. O'Dea, MT, ASCP Histology Supervisor Highland Hospital 1000 South Ave. Rochester, New York 14620 office 585.341.6596 lab 585.341.8314 elise_odea at urmc.rochester.edu Confidentiality Notice This transmission contains confidential information protected by New York State law and HIPAA regulations. You are prohibited from making any further disclosure of this information without the specific written consent of the person to whom it pertains, or as otherwise permitted by law. A general authorization for the release of medical or other information is not sufficient authorization for further disclosure of information, which is protected by New York State Public Health Law Article 27-F or Title 42 of the Code of Federal Regulations. Any unauthorized further disclosure in violation of State law may result in a fine or jail sentence or both. If you have received this material in error, please notify the sender IMMEDIATELY to arrange for the return or destruction of the document(s). ------------------------------ Message: 2 Date: Mon, 12 Oct 2015 19:15:02 +0000 (UTC) From: Rene J Buesa To: "ODea, Elise" , "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] Microtomy statisitcs Message-ID: <932792051.2671484.1444677302157.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 24Ren? J. On Monday, October 12, 2015 1:29 PM, "ODea, Elise via Histonet" wrote: I imagine there are several schools of thought on this topic, but here goes:? How many blocks should a histotech cut for 1 H&E per hour?? Other duties during this time are drying, loading and unloading a stainer and coverslipper. Thanks! Elise Elise T. O'Dea, MT, ASCP Histology Supervisor Highland Hospital 1000 South Ave. Rochester, New York 14620 office 585.341.6596 lab 585.341.8314 elise_odea at urmc.rochester.edu Confidentiality Notice This transmission contains confidential information protected by New York State law and HIPAA regulations. You are prohibited from making any further disclosure of this information without the specific written consent of the person to whom it pertains, or as otherwise permitted by law. A general authorization for the release of medical or other information is not sufficient authorization for further disclosure of information, which is protected by New York State Public Health Law Article 27-F or Title 42 of the Code of Federal Regulations. Any unauthorized further disclosure in violation of State law may result in a fine or jail sentence or both. If you have received this material in error, please notify the sender IMMEDIATELY to arrange for the return or destruction of the document(s). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 12 Oct 2015 13:26:24 -0700 From: P Sicurello To: Rene J Buesa Cc: "ODea, Elise" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Microtomy statisitcs Message-ID: Content-Type: text/plain; charset=UTF-8 Rene' has a really good paper that discusses this topic. http://www.histosearch.com/ADP9ProductivityStandards.pdf The larger issues are how to track the number cut per hour. It's easy to get a daily rate, but breaking it down to blocks/hour when techs are doing multiple tasks makes it a bit more challenging. Are people willing to share how they track the number of blocks cut per hour per tech? Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UCSan Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Mon, Oct 12, 2015 at 12:15 PM, Rene J Buesa via Histonet < histonet at lists.utsouthwestern.edu> wrote: > 24Ren? J. > > > On Monday, October 12, 2015 1:29 PM, "ODea, Elise via Histonet" < > histonet at lists.utsouthwestern.edu> wrote: > > > I imagine there are several schools of thought on this topic, but > here > goes: How many blocks should a histotech cut for 1 H&E per hour? > Other duties during this time are drying, loading and unloading a > stainer and coverslipper. > > Thanks! > Elise > Elise T. O'Dea, MT, ASCP > Histology Supervisor > Highland Hospital > 1000 South Ave. > Rochester, New York 14620 > office 585.341.6596 > lab 585.341.8314 > elise_odea at urmc.rochester.edu > > > Confidentiality Notice > This transmission contains confidential information protected by New > York State law and HIPAA regulations. You are prohibited from making > any further disclosure of this information without the specific > written consent of the person to whom it pertains, or as otherwise > permitted by law. A general authorization for the release of medical > or other information is not sufficient authorization for further > disclosure of information, which is protected by New York State Public > Health Law Article 27-F or Title 42 of the Code of Federal > Regulations. Any unauthorized further disclosure in violation of State > law may result in a fine or jail sentence or both. If you have > received this material in error, please notify the sender IMMEDIATELY to arrange for the return or destruction of the document(s). > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 143, Issue 10 ***************************************** From keith_kim2015 at yahoo.com Tue Oct 13 14:19:41 2015 From: keith_kim2015 at yahoo.com (Kim) Date: Tue, 13 Oct 2015 14:19:41 -0500 Subject: [Histonet] Histonet Digest, Vol 143, Issue 10 In-Reply-To: References: Message-ID: <99FBF096-78D2-48C9-95E7-DF912EA46682@yahoo.com> Unsubscribe Sent from the iPhone of Kim Mann ?? > On Oct 13, 2015, at 12:00 PM, histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Microtomy statisitcs (ODea, Elise) > 2. Re: Microtomy statisitcs (Rene J Buesa) > 3. Re: Microtomy statisitcs (P Sicurello) > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun at hhchealth.org Tue Oct 13 15:02:46 2015 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 13 Oct 2015 20:02:46 +0000 Subject: [Histonet] Shipping paraffin blocks overseas Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B057AB4@HHCEXCHMB03.hhcsystem.org> It's been a long time since I have sent a paraffin tissue block out of the country. Can someone bring me up-to-date on requirements for shipping paraffin tissue blocks overseas (specifically Germany). I believe FedEx will be used for the shipping. Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From JDASILVAMARTINS at mgh.harvard.edu Tue Oct 13 15:51:29 2015 From: JDASILVAMARTINS at mgh.harvard.edu (Da Silva Martins, Janaina) Date: Tue, 13 Oct 2015 20:51:29 +0000 Subject: [Histonet] Intravital confocal microscopy Message-ID: Hello Somebody knows who is doing histology in alive mouse in USA territory? I'm looking for somebody with experience in bone intravital microscopy in USA! Thanks Janaina The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From Richard.Cartun at hhchealth.org Wed Oct 14 11:29:49 2015 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Wed, 14 Oct 2015 16:29:49 +0000 Subject: [Histonet] Sunquest CoPath Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B057CB7@HHCEXCHMB03.hhcsystem.org> Dear Histology Colleagues: I need your help once again. Our healthcare system is in the process of switching over to Sunquest CoPath; we currently have Cerner CoPath now. I have been told that when using their bar code and tracking system one cannot generate unstained slides (for possible histochemical stains and/or IHC testing) upfront (at the time that the H&Es are cut). Evidently, this is because the unique identifier for that unstained slide cannot be modified for ancillary testing. Is this true? I cannot believe that a software program for pathology laboratories in this day and age would not allow for this. Is anyone using this system and have you found a solution to this problem? We have a lot of slide protocols that include the cutting of unstained slides upfront. I apologize if I have incorrectly stated the facts here; however, this is how it was explained to me in a conference call earlier this week. As always, thank you for sharing your knowledge and experiences. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From KSimeone at leavittmgt.com Wed Oct 14 12:24:28 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Wed, 14 Oct 2015 17:24:28 +0000 Subject: [Histonet] FULLTIME DAY SHIFT (first shift) AND FULL TIME NIGHT SHIFT (IHC) POSITION DELRAY BCH FL In-Reply-To: <43944B1DBAAC2846B7B9D626B5F1233C4D2DE034@vm-email.leavittmgt.com> References: <43944B1DBAAC2846B7B9D626B5F1233C4D2DDB61@vm-email.leavittmgt.com>, <43944B1DBAAC2846B7B9D626B5F1233C4D2DDDED@vm-email.leavittmgt.com>, <43944B1DBAAC2846B7B9D626B5F1233C4D2DE034@vm-email.leavittmgt.com> Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C4D2DE159@vm-email.leavittmgt.com> Hi Histonetters! We are looking for TWO full time licensed histotechs here in our very busy Delray Beach, Florida dermatology laboratory. These are permanent full time, (40 hours) position with benefits (medical/401k/vacation). Annual salary in the $50k range (NIGHT SHIFT DIFF AVAILABLE). THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! DAYTIME POSTION: *full time position Mon-Fri 8a-5:30p (start time may vary) *MUST be licensed as a FLORIDA HISTOTECHNICIAN OR HISTOTECHNOLOGIST (NO pending licensure pls) *PREFER experience but WILL TRAIN a knowledgeable, willing candidate *duties to include (but not limited to): grossing, microtomy, accessioning, embedding and general histology *must be self motivated, reliable and a team player DAYTIME SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY: https://advancedderm.applicantpro.com/jobs/240090.html NIGHT POSITION: *Full time position Mon-Fri 10:30p-7:00a (IMMUNOHISTOCHEMISTRY/SPECIALS department) *MUST be licensed as a FLORIDA HISTOTECHNOLOGIST (this is NOT negotiable) *EXPERIENCE WITH IHC A MUST! Leica (BOND) and Roche/Ventana equipment experience preferred *Must be able to multi-task special stains *MUST have at LEAST 2 years experience. Please DO NOT respond if you do not possess EXPERIENCE in this area! *must be confidant, quick learner, self motivated, reliable and a team player NIGHT SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY: https://advancedderm.applicantpro.com/jobs/278020.html Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From KSimeone at leavittmgt.com Wed Oct 14 12:42:58 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Wed, 14 Oct 2015 17:42:58 +0000 Subject: [Histonet] Microtomy statisitcs (White, Lisa M.) Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C4D2DE166@vm-email.leavittmgt.com> We timed about 5 techs cutting multiple blocks (shaves/excisions) and made an average from those times. Not everyone cuts at the same speed and technique varies. An 'average' range is what our techs are expected to fall into. Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor Alternate Laboratory Supervisor Delray Beach Technical Laboratory ADCS Clinics, LLC The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From Elise_ODea at URMC.Rochester.edu Wed Oct 14 13:09:40 2015 From: Elise_ODea at URMC.Rochester.edu (ODea, Elise) Date: Wed, 14 Oct 2015 18:09:40 +0000 Subject: [Histonet] (no subject) Message-ID: <6e07ec4e9815496a97217f6a0ffdc513@exmbxpdc06.urmc-sh.rochester.edu> Thanks for your input. I am thankful we do not have as many disruptions as your lab! Some sort of guidelines are necessary to evaluate in some labs for new histotechs to aspire to. Elise From greg.dobbin at gmail.com Wed Oct 14 13:36:01 2015 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Wed, 14 Oct 2015 15:36:01 -0300 Subject: [Histonet] Microtomy Statistics Message-ID: Are you hoping to measure competency or productivity? If it is competency that you hope to assess, allow your tech to cut blocks of a variety of specimen types for 1 hour *without* distractions. If they cut 30 or more blocks and produce good quality sections, then they are meeting your standard. If it is productivity that you want to assess, then you may want to look at an average of several daily totals per tech. If you need to establish what the desired minimal productivity benchmark is for your lab, do this for several or all techs and come up with a reasonable (and achievable) benchmark for all to strive for as their minimum. Cheers, Greg -- Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 *Everything in moderation...even moderation itself**!* From JefThompson at salud.unm.edu Wed Oct 14 13:42:07 2015 From: JefThompson at salud.unm.edu (Jeffrey Thompson) Date: Wed, 14 Oct 2015 18:42:07 +0000 Subject: [Histonet] ph electrode and PFA Message-ID: <1444848126973.22810@salud.unm.edu> Hello, I've read several protocols regarding preparation of 4% paraformaldehyde. I have a question regarding the pH determination. Some protocls say to use pH test strips because PFA will 'ruin the pH meter' (presumably the electrode), some just say to use strips and most say nothing about how to measure pH. I've come across some equivocal mentions of PFA damaging polymer barrel electrodes but not glass. My questions: does paraformaldehyde solution preparation damage pH electrodes and if so, how? Any replies would be most welcome Thanks From brendal.finlay at medicalcenterclinic.com Wed Oct 14 13:48:52 2015 From: brendal.finlay at medicalcenterclinic.com (Brendal Finlay) Date: Wed, 14 Oct 2015 13:48:52 -0500 Subject: [Histonet] FW: Histotechnologist - Histology - Apply Now Message-ID: <000601d106b0$f8d73840$ea85a8c0$@finlay@medicalcenterclinic.com> We have a full time Histotechnologist position available in NW Florida. Please see the link below for more information and to apply. Thank you! Apply to Dental Assistant / EFDA / DA / CDA / RDA, Medical Assistant - Various Departments, Service Technician, and more. CareerBuilder.com Email a Job brendal.finlay at medicalcenterclinic.com sent you this job from CareerBuilder.com: Histotechnologist - Histology Medical Center Clinic - US: Pensacola, FL Apply Now For your privacy and protection, when applying to a job online: Never give out your social security number, credit card or bank account information; or perform any sort of monetary transaction. Please do not reply to this email. This is an automated email. If you reply, it cannot be read. If you have questions or comments for CareerBuilder.com please contact Customer Service. 5550-A Peachtree Parkway, Suite 200 | Norcross GA 30092 From TanyaAbbott at catholichealth.net Wed Oct 14 15:35:23 2015 From: TanyaAbbott at catholichealth.net (Abbott, Tanya) Date: Wed, 14 Oct 2015 20:35:23 +0000 Subject: [Histonet] VIP5 Message-ID: <852F7D2C14FB464D80E182B15DB138AF7B564802@CHIEX005.CHI.catholichealth.net> Help!!! We changed our processor on Monday, all reagents. Run Monday into Tuesday a.m. was fine. Tuesday nights run had some blocks in it that weren't fully processed; chamber looked like it had droplets of residual water (or maybe alcohol?) in it. Dumped and refilled everything, ran a few test blocks, same scenario. Any suggestions?! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology Penn State Health St. Joseph (phone) 610-378-2635 From saby_joseph_a at yahoo.com Wed Oct 14 18:44:35 2015 From: saby_joseph_a at yahoo.com (Joseph Saby) Date: Wed, 14 Oct 2015 23:44:35 +0000 (UTC) Subject: [Histonet] VIP5 In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF7B564802@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF7B564802@CHIEX005.CHI.catholichealth.net> Message-ID: <1455273922.534699.1444866276010.JavaMail.yahoo@mail.yahoo.com> We had this happen when the individual changing the processor reversed order of the alcohols, placing the newest first and the oldest last.? Hope your answer is as simple, even if it is embarrassing. Joe Saby From: "Abbott, Tanya via Histonet" To: "histonet at lists.utsouthwestern.edu" Sent: Wednesday, October 14, 2015 4:35 PM Subject: [Histonet] VIP5 Help!!! We changed our processor on Monday, all reagents. Run Monday into Tuesday a.m. was fine. Tuesday nights run had some blocks in it that weren't fully processed; chamber looked like it had droplets of residual water (or maybe alcohol?) in it. Dumped and refilled everything, ran a few test blocks, same scenario. Any suggestions?! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology Penn State Health St. Joseph (phone) 610-378-2635 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mark.Pawlowski at jefferson.edu Thu Oct 15 08:30:36 2015 From: Mark.Pawlowski at jefferson.edu (Mark Pawlowski) Date: Thu, 15 Oct 2015 13:30:36 +0000 Subject: [Histonet] Mohs tech in Philly Message-ID: Hello, We at Jefferson Dermatology have an opening for a Mohs tech, with your time to be split betweeen our Philadelphia, PA and Wilmington, DE locations. Please reply to mark.pawlowski at jefferson.edu. The information contained in this transmission contains privileged and confidential information. It is intended only for the use of the person named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. CAUTION: Intended recipients should NOT use email communication for emergent or urgent health care matters. From ASelf at tidelandshealth.org Thu Oct 15 10:18:39 2015 From: ASelf at tidelandshealth.org (Amy Self) Date: Thu, 15 Oct 2015 11:18:39 -0400 Subject: [Histonet] Pathology labs using Premier for staffing Message-ID: Dear Histonetters, Are there any labs that utilize Premier for staffing productivity? For those that do use Premier is the histology section separated from your other lab sections or included with the clinical lab? And also is cytology considered separate or included with histology? Do you report volume and staffing? Or, only report one or the other? I am trying to find a comparison group for staffing productivity. Our histology and cytology sections are included as part of the entire lab for both volume and staffing. Thanks in advance for your help, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From bethcoxx at gmail.com Thu Oct 15 13:50:26 2015 From: bethcoxx at gmail.com (Beth Cox) Date: Thu, 15 Oct 2015 14:50:26 -0400 Subject: [Histonet] Pathology labs using Premier for staffing Message-ID: <561FF572.3070603@gmail.com> The way Premier can break down the productivity/staffing info for your lab is dependent on how your organization separates the lab into cost centers -- if your lab is all one cost center for clinical and anatomic labs together, that's the way it gets reported to Premier, and that's how the statistics will come back to you. These statistics are based on billable tests and worked hours for your cost center. If your organization has the anatomic lab as a different cost center from the clinical lab, the info will get reported to Premier separately and Premier can give you info back comparing you to other AP only databases. There are no labs I am aware of which have Histo and Cyto in separate cost centers, so Premier would likely be unable to give you comparison data that way. Beth Cox, HTL/SCT(ASCP)QIHC Pathology Solutions, Inc Message: 10 Date: Thu, 15 Oct 2015 11:18:39 -0400 From: Amy Self To:"histonet at lists.utsouthwestern.edu" Subject: [Histonet] Pathology labs using Premier for staffing Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Histonetters, Are there any labs that utilize Premier for staffing productivity? For those that do use Premier is the histology section separated from your other lab sections or included with the clinical lab? And also is cytology considered separate or included with histology? Do you report volume and staffing? Or, only report one or the other? I am trying to find a comparison group for staffing productivity. Our histology and cytology sections are included as part of the entire lab for both volume and staffing. Thanks in advance for your help, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org From md at personifysearch.com Thu Oct 15 14:04:05 2015 From: md at personifysearch.com (Mary Southgate Dickson) Date: Thu, 15 Oct 2015 15:04:05 -0400 Subject: [Histonet] Histology Specialist Opportunity- Boston Message-ID: <7A483360-E56E-4EBF-998F-DC0F49631882@personifysearch.com> Hi All! We have recently had a top client open a new field based histology specialist opportunity to support their customers in the Northeast. The ideal candidate will have a 4 year degree and a strong histology background. The position is field based and the ideal location would be in the Boston area. Please email me at md at personifysearch.com for more information. Thanks! Mary Southgate Mary Southgate Dickson Talent Management Executive Personify 401 Harrison Oaks Boulevard Suite 350 Cary, North Carolina 27513 www.personifysearch.com 800.875.6188 x 130 http://www.linkedin.com/in/marysouthgatedickson From lins0701 at gmail.com Thu Oct 15 14:14:24 2015 From: lins0701 at gmail.com (Sophia Lin) Date: Thu, 15 Oct 2015 12:14:24 -0700 Subject: [Histonet] Histotechnician FT Murrieta Message-ID: Small established GI lab in Murrieta seeking a part time/full time histotechnician or histotechnologist (certified only). Must have experience with VIP processors, microtomy, stains and hand coverslipping glass slides. Also must have technical skills and ability to troubleshoot stains and machines. Serious inquiries only to lins0701 at gmail.com Thanks! From rachel at gbi-inc.com Fri Oct 16 13:56:15 2015 From: rachel at gbi-inc.com (Rachel M Gonzalez) Date: Fri, 16 Oct 2015 14:56:15 -0400 Subject: [Histonet] IDO1 / IDO is indoleamine 2,3-dioxygenase antibody Message-ID: Does anyone know of a good IDO1 antibody for FFPE human tissue? IVD certified would be best but okay if not. thanks Rachel From blayjorge at gmail.com Fri Oct 16 15:34:39 2015 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Fri, 16 Oct 2015 16:34:39 -0400 Subject: [Histonet] Getting rid of bubbles in a gelatinous slide mounting medium Message-ID: Hello Histonetters: I have several hundred temporary slide preparations of insects body parts mounted on aqueous gelatin. As we prepare to photograph, we have been told to completely cover the specimen with the gelatin mounting and place a cove slip on top. As we melt new gelatin to comply, the process is invariably creating annoying bubbles, regardless of how gently we heat the gelatin. If we stick to gelatin, can someone suggest a method to minimize bubbles other than manually removing them? Of we switch mounting medium remember, all specimens are temp. mounted i n the gelatin), what aqueous medium compatible with the aqueous gelatin would you recommend that does not (ideally) create bubbles (ideally cheap). If you have any suggestions, please feel free to email me directly: blayjorge at gmail.com Gratefully, Jorge P.S. Apologies for potentially duplicate emails. Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From koellingr at comcast.net Fri Oct 16 16:37:31 2015 From: koellingr at comcast.net (koellingr at comcast.net) Date: Fri, 16 Oct 2015 21:37:31 +0000 (UTC) Subject: [Histonet] Getting rid of bubbles in a gelatinous slide mounting medium In-Reply-To: References: Message-ID: <1415974593.3013440.1445031451756.JavaMail.zimbra@comcast.net> Have never used the following method for gelatin microscope slides but maybe it would work????? When pouring acrylamide gels or setting up a resin matrix column I would always de-gas the liquid in a vacuum set-up of some kind.? Placing a liquid in the vacuum not only gets rid of oxygen which can interfere with products being separated but also gets rid of small gas bubbles that can interfere with constant and continuous flow of what should be a homogenous gel.? Degas the gelatin and use it quickly and maybe put the slides themselves under vacuum.? At Smithsonian they should have all kinds of vacuum set-ups.? Interested if it works; I have no idea. Ray (Lake Forest Park, WA) ----- Original Message ----- From: "Jorge A. Santiago-Blay via Histonet" To: Histonet at lists.utsouthwestern.edu Sent: Friday, October 16, 2015 1:34:39 PM Subject: [Histonet] Getting rid of bubbles in a gelatinous slide mounting????????medium Hello Histonetters: I have several hundred temporary slide preparations of insects body parts mounted on aqueous gelatin. As we prepare to photograph, we have been told to completely cover the specimen with the gelatin mounting and place a cove slip on top. As we melt new gelatin to comply, the process is invariably creating annoying bubbles, regardless of how gently we heat the gelatin. If we stick to gelatin, can someone suggest a method to minimize bubbles other than manually removing them? Of we switch mounting medium remember, all specimens are temp. mounted i n the gelatin), what aqueous medium compatible with the aqueous gelatin would you recommend that does not (ideally) create bubbles (ideally cheap). If you have any suggestions, please feel free to email me directly: blayjorge at gmail.com Gratefully, Jorge P.S. Apologies for potentially duplicate emails. Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght at yahoo.com Sat Oct 17 13:01:49 2015 From: tkngflght at yahoo.com (Cheryl) Date: Sat, 17 Oct 2015 18:01:49 +0000 (UTC) Subject: [Histonet] aqueous mounting media (gelatin) In-Reply-To: References: Message-ID: <605010122.1869920.1445104909346.JavaMail.yahoo@mail.yahoo.com> Hi Jorge- There are numerous commercially prepared aqueous-based mounting medium. ?Most clinical versions are used for immunoflorescent staining. ?They're ExPEnSiVe!! ? When we use them we dip the slide or add a drop or two of the most recent rinse solution. ?For our IF mounts (also temporary) we dip in Ventana's reagent buffer so there is quite a bit of liquid on the slide, then drip a few drops of mounting media right on top of the tissue (centered) and drop the coverslip on flat (not from the edge).? Press down in the center right over the tissue with a gloved finger.?This pressure from a finger drives the more mobile components (bubbles) to the edges away from the tissue we want to see. It's messy-- you get all sorts of goop out the side of the slides, but these are easily cleaned with a gauze pad soaked in buffer and then left laying flat to dry for a little bit. Additionally-- thin your gelatin a little so it doesn't get fully firm when cool - with a temp mount the additional liquid won't make any difference except to allow your bubbles to move more freely. ?If you need them to last longer without evaporating, you can seal the edges of the slides with fingernail polish or regular mounting media (resinous) left to fully dry.? Hope this helps!?Cheryl Kerry, HT(ASCP)? Full Staff Inc. ???? ? From elonergan at metrocast.net Sun Oct 18 17:15:38 2015 From: elonergan at metrocast.net (elonergan at metrocast.net) Date: Sun, 18 Oct 2015 15:15:38 -0700 Subject: [Histonet] Fw: new message Message-ID: <000005270af2$57c1a3b1$fccba814$@metrocast.net> Hello! New message, please read elonergan at metrocast.net From elonergan at metrocast.net Sun Oct 18 17:12:45 2015 From: elonergan at metrocast.net (Duraine Lita via Histonet) Date: Sun, 18 Oct 2015 15:12:45 -0700 Subject: [Histonet] Fw: new message Message-ID: <00006c05d023$2a66f455$ea5b406c$@metrocast.net> Hello! New message, please read Duraine Lita via Histonet From elonergan at metrocast.net Sun Oct 18 17:12:45 2015 From: elonergan at metrocast.net (Duraine Lita via Histonet) Date: Sun, 18 Oct 2015 15:12:45 -0700 Subject: [Histonet] Fw: new message Message-ID: <00006c05d023$2a66f455$ea5b406c$@metrocast.net> Hello! New message, please read Duraine Lita via Histonet From elonergan at metrocast.net Sun Oct 18 17:12:37 2015 From: elonergan at metrocast.net (Duraine Lita via Histonet) Date: Sun, 18 Oct 2015 15:12:37 -0700 Subject: [Histonet] Fw: new message Message-ID: <00001f37827c$8ab6b1b5$cf81f546$@metrocast.net> Hello! New message, please read Duraine Lita via Histonet From elonergan at metrocast.net Sun Oct 18 17:12:45 2015 From: elonergan at metrocast.net (Duraine Lita via Histonet) Date: Sun, 18 Oct 2015 15:12:45 -0700 Subject: [Histonet] Fw: new message Message-ID: <00006c05d023$2a66f455$ea5b406c$@metrocast.net> Hello! New message, please read Duraine Lita via Histonet From elonergan at metrocast.net Sun Oct 18 17:12:50 2015 From: elonergan at metrocast.net (Duraine Lita via Histonet) Date: Sun, 18 Oct 2015 15:12:50 -0700 Subject: [Histonet] Fw: new message Message-ID: <0000bde65696$6331a182$550dd42b$@metrocast.net> Hello! New message, please read Duraine Lita via Histonet From elonergan at metrocast.net Sun Oct 18 17:12:50 2015 From: elonergan at metrocast.net (Duraine Lita via Histonet) Date: Sun, 18 Oct 2015 15:12:50 -0700 Subject: [Histonet] Fw: new message Message-ID: <0000bde65696$6331a182$550dd42b$@metrocast.net> Hello! New message, please read Duraine Lita via Histonet From elonergan at metrocast.net Sun Oct 18 17:12:37 2015 From: elonergan at metrocast.net (Duraine Lita via Histonet) Date: Sun, 18 Oct 2015 15:12:37 -0700 Subject: [Histonet] Fw: new message Message-ID: <00001f37827c$8ab6b1b5$cf81f546$@metrocast.net> Hello! New message, please read Duraine Lita via Histonet From elonergan at metrocast.net Sun Oct 18 17:12:37 2015 From: elonergan at metrocast.net (Duraine Lita via Histonet) Date: Sun, 18 Oct 2015 15:12:37 -0700 Subject: [Histonet] Fw: new message Message-ID: <00001f37827c$8ab6b1b5$cf81f546$@metrocast.net> Hello! New message, please read Duraine Lita via Histonet From elonergan at metrocast.net Sun Oct 18 17:12:50 2015 From: elonergan at metrocast.net (Duraine Lita via Histonet) Date: Sun, 18 Oct 2015 15:12:50 -0700 Subject: [Histonet] Fw: new message Message-ID: <0000bde65696$6331a182$550dd42b$@metrocast.net> Hello! New message, please read Duraine Lita via Histonet From elonergan at metrocast.net Sun Oct 18 17:13:40 2015 From: elonergan at metrocast.net (Duraine Lita via Histonet) Date: Sun, 18 Oct 2015 15:13:40 -0700 Subject: [Histonet] Fw: new message Message-ID: <00007d3c4811$b5c070fb$9597d69d$@metrocast.net> Hello! New message, please read Duraine Lita via Histonet From elonergan at metrocast.net Sun Oct 18 17:13:36 2015 From: elonergan at metrocast.net (Duraine Lita via Histonet) Date: Sun, 18 Oct 2015 15:13:36 -0700 Subject: [Histonet] Fw: new message Message-ID: <00008c0ba4a3$a7a6f57c$7f608351$@metrocast.net> Hello! New message, please read Duraine Lita via Histonet From elonergan at metrocast.net Sun Oct 18 17:13:31 2015 From: elonergan at metrocast.net (Duraine Lita via Histonet) Date: Sun, 18 Oct 2015 15:13:31 -0700 Subject: [Histonet] Fw: new message Message-ID: <000031031572$41f4e8ce$f3f59f50$@metrocast.net> Hello! New message, please read Duraine Lita via Histonet From elonergan at metrocast.net Sun Oct 18 17:15:35 2015 From: elonergan at metrocast.net (elonergan at metrocast.net) Date: Sun, 18 Oct 2015 15:15:35 -0700 Subject: [Histonet] Fw: new message Message-ID: <0000dc360e39$ec353e67$d5b8f7ea$@metrocast.net> Hello! New message, please read elonergan at metrocast.net From elonergan at metrocast.net Sun Oct 18 17:15:47 2015 From: elonergan at metrocast.net (elonergan at metrocast.net) Date: Sun, 18 Oct 2015 15:15:47 -0700 Subject: [Histonet] Fw: new message Message-ID: <00003916e26a$926f33c5$d1a33ac3$@metrocast.net> Hello! New message, please read elonergan at metrocast.net From elonergan at metrocast.net Sun Oct 18 17:15:43 2015 From: elonergan at metrocast.net (elonergan at metrocast.net) Date: Sun, 18 Oct 2015 15:15:43 -0700 Subject: [Histonet] Fw: new message Message-ID: <00000d487afd$3505039d$c813f12d$@metrocast.net> Hello! New message, please read elonergan at metrocast.net From elonergan at metrocast.net Sun Oct 18 17:15:51 2015 From: elonergan at metrocast.net (elonergan at metrocast.net) Date: Sun, 18 Oct 2015 15:15:51 -0700 Subject: [Histonet] Fw: new message Message-ID: <0000148db17b$ec6ca1cd$769f4e1b$@metrocast.net> Hello! New message, please read elonergan at metrocast.net From elonergan at metrocast.net Sun Oct 18 17:12:30 2015 From: elonergan at metrocast.net (Michael Ann Jones via Histonet) Date: Sun, 18 Oct 2015 15:12:30 -0700 Subject: [Histonet] Fw: new message Message-ID: <00004545d316$c1db2449$deb220c0$@metrocast.net> Hello! New message, please read Michael Ann Jones via Histonet From elonergan at metrocast.net Sun Oct 18 17:12:44 2015 From: elonergan at metrocast.net (Michael Ann Jones via Histonet) Date: Sun, 18 Oct 2015 15:12:44 -0700 Subject: [Histonet] Fw: new message Message-ID: <00007358252b$31d6851f$36e9e6a8$@metrocast.net> Hello! New message, please read Michael Ann Jones via Histonet From elonergan at metrocast.net Sun Oct 18 17:12:15 2015 From: elonergan at metrocast.net (Michael Ann Jones via Histonet) Date: Sun, 18 Oct 2015 15:12:15 -0700 Subject: [Histonet] Fw: new message Message-ID: <0000f0640872$f3d0e4da$89af2661$@metrocast.net> Hello! New message, please read Michael Ann Jones via Histonet From elonergan at metrocast.net Sun Oct 18 17:12:25 2015 From: elonergan at metrocast.net (Michael Ann Jones via Histonet) Date: Sun, 18 Oct 2015 15:12:25 -0700 Subject: [Histonet] Fw: new message Message-ID: <0000a4cf2bac$f0651d31$90064e1f$@metrocast.net> Hello! New message, please read Michael Ann Jones via Histonet From ASelf at tidelandshealth.org Mon Oct 19 13:42:53 2015 From: ASelf at tidelandshealth.org (Amy Self) Date: Mon, 19 Oct 2015 14:42:53 -0400 Subject: [Histonet] Meditech Client Server Message-ID: Happy Monday Histonetters, Question: Does anyone out there in Histoland use Meditech Client Server for pathology? If so how do you like it and do you find that it provides histology and/or cytology with everything needed to operate the department to full capacity especially QA/QC tracking? Thanks, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From jrmelectric at att.net Tue Oct 20 07:18:50 2015 From: jrmelectric at att.net (james mcmurry) Date: Tue, 20 Oct 2015 05:18:50 -0700 Subject: [Histonet] Help with "Quotes" for presentation Message-ID: <1445343530.19914.YahooMailBasic@web126203.mail.ne1.yahoo.com> Hello Fellow Histo techs, This is my first time posting to Histonet. I am looking for experienced individuals to provide me with a quote (very short 1-2 sentences) on what you feel has changed most in histology over the years. I am presenting at the MSH fall symposium and as part of my presentation I would like to include several slides of quotes from Histotechs. My presentation is called "A walk down memory lane". I am a brand new tech just getting her feet wet and wanted to talk to other about the changes in histology. Until I started talking to some techs with 20-30+ years experience I never knew how things were done years ago. I found it very interesting to say the least. Here is a very general example on what I am looking for but please use this similair format: For me the greatest change is histology has been the increase in personalized care. Next-Gen Sequencing is really changing how we treat a patients exact cancer. Lisa M. McMurry HT (Henry Ford Health System, Detroit-MI) It could be on microtomes, embedding, special stains, tech duties, molecular or just anything histology related. I am hoping to get 4-6 good quotes. I hope you find a minute to shoot me back a email. Thank you histo buddies for all your help!! Lisa McMurry BS, LVT, HT jrmelectric at att.net From ian.bernard at comcast.net Tue Oct 20 12:35:45 2015 From: ian.bernard at comcast.net (ian bernard) Date: Tue, 20 Oct 2015 11:35:45 -0600 Subject: [Histonet] Inspection (External or Self) Standards Message-ID: Are there any laboratory standards (CLIA,CAP, other etc.) that I can reference concerning inspecting or self-inspecting a certain number of reagents for labeling/expiration dates that will be a sufficient representation of the whole (% or number). Also standards for how many monthly QC temp/function checks are acceptable for compliance (i.e. 100% or what acceptable margin of error). Six Sigma which is a quality concept allows for 3.4 defects per million opportunities. Your thoughts or references? V/r Ian R. Bernard, MSHA, HT (ASCP) HTL (Pend-2015) USAF, MSgt (Retired) Anatomic Pathology Technical Supervisor 10th Medical Group 210-687-7540 Cell ian.bernard at comcast.net ian.bernard.3 at us.af.mil University of Alabama Birmingham- Alumni. From mbireir at yahoo.com Tue Oct 20 13:08:13 2015 From: mbireir at yahoo.com (Manahil) Date: Tue, 20 Oct 2015 22:08:13 +0400 Subject: [Histonet] Grossing big specimens Message-ID: <4E81DFE9-8C17-458B-8910-9E600590E821@yahoo.com> Hi histonet, I have a query about who is grossing the placenta and stomach sleeve? Is it tech. or pathologist? And what is CAP recommendation. Thanks in advance for feed back. Manahil Sent from my iPhone From jvickroy at SpringfieldClinic.com Tue Oct 20 14:27:18 2015 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Tue, 20 Oct 2015 19:27:18 +0000 Subject: [Histonet] Small grossing station Message-ID: <9B1A1501A800064397369BD8072E6BCA064A46A0@E2K10DB.springfieldclinic.com> We have outgrown our two small specimen grossing stations and our looking into finding a small system for a counter that will have a formaldehyde filter. Trying to make sure it is safe but not wanting to spend a lot of money. Station will be for small gi biopsies. Just need something to keep the formalin fumes within safe limits. Any suggestions? Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From karla at dermatopathologynorthwest.com Tue Oct 20 15:24:53 2015 From: karla at dermatopathologynorthwest.com (Karla) Date: Tue, 20 Oct 2015 13:24:53 -0700 Subject: [Histonet] lifespan of equipment Message-ID: <000001d10b75$61798c90$246ca5b0$@com> Hi Histonetters, In an attempt to be forward thinking and budget for what we need, I am looking for information on the average lifespan (or how often it should be replaced) of individual pieces of histology equipment such as processors, microtomes , stainers, embedding centers and the like. Has anyone compiled info that they would be willing to share? I realize that vendors have specific info for their instruments but, I am looking for generalized information. By the way, I am still using VIP K tissue processor that is more than 30 years old!!! Thanks in advance, Karla Carlmas Dermatopathology Northwest 2330 130th Ave NE Ste. 201 Bellevue, WA 98005 karla at dermatopathologynorthwest.com From ASelf at tidelandshealth.org Wed Oct 21 06:46:49 2015 From: ASelf at tidelandshealth.org (Amy Self) Date: Wed, 21 Oct 2015 07:46:49 -0400 Subject: [Histonet] Meditech Client Server Message-ID: Dear Histonetters, Question: Does anyone out there in Histoland use Meditech Client Server for pathology? If so how do you like it and do you find that it provides histology and/or cytology with everything needed to operate the department to full capacity especially QA/QC tracking? Thanks in advance for your help, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From akbitting at geisinger.edu Wed Oct 21 08:17:10 2015 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Wed, 21 Oct 2015 13:17:10 +0000 Subject: [Histonet] Phosphorylase stain for muscle Message-ID: <37990cbf57d94cde8c497df08e21dd44@LOFEXMBX108W12V.geisinger.edu> Can anyone tell me what kind of glycogen to use for this? Sigma's website has multiple choices. Thanks. Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From relia1 at earthlink.net Wed Oct 21 09:49:36 2015 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 21 Oct 2015 10:49:36 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin 10-21-2015 Exciting opportunities Coast to Coast!! Message-ID: <00a501d10c0f$b50ff660$1f2fe320$@earthlink.net> Hi Histonetters! Coast to Coast the histology field is blossoming again!! If you were looking for a new opportunity but the timing wasn't right or the location wasn't right or the employers were moving waaaay toooo SLOW. Well things are different now!! I am very excited about the positions I am working on because my clients offer excellent compensation, really nice benefits and great working environments. These are confirmed immediate full time permanent positions!! If you are ASCP HT/HTL certified with 2-5 years of experience, my clients want to talk to you ASAP!!! If you are interested please contact me. Send me an email at relia1 at earthlink.net , or call toll free at the office at 866-607-3542 or on my cell at (407)353-5070. Here are the positions: Histology Manager - Stockton, CA Assistant Supervisor - Roswell, NM Histotechnologist - Los Angeles, CA *New Position! Tech Support Specialist - Southern CA *New Position! Histotech - Modesto, CA Histotechnician - Ann Arbor, MI *New Position! Histotech Nashville Senior Histotech - Atlanta Dermpath histotech - Longview, TX Dermpath Histotech - Fayetteville AR If you know someone who might be interested please feel free to pass the information along to them as well. I remember I offer a 250.00 referral fee if I place someone that you refer to me. Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From fourfonners at yahoo.com Wed Oct 21 10:05:40 2015 From: fourfonners at yahoo.com (Sheila Fonner) Date: Wed, 21 Oct 2015 15:05:40 +0000 (UTC) Subject: [Histonet] HPV ISH References: <1895096927.773493.1445439940413.JavaMail.yahoo@mail.yahoo.com> Message-ID: <1895096927.773493.1445439940413.JavaMail.yahoo@mail.yahoo.com> ? Good morning histonetters, Is there anyone out there who is currently doing ISH HPV probes on the Leica Bond?? If so, where are you getting your tissue for positive controls?? Any help would be greatly appreciated. Thanks SheilaKnoxville, TN From KSimeone at leavittmgt.com Wed Oct 21 12:16:57 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Wed, 21 Oct 2015 17:16:57 +0000 Subject: [Histonet] FULLTIME DAY SHIFT (first shift) AND FULL TIME NIGHT SHIFT (IHC) POSITION DELRAY BCH FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C4D2DE619@vm-email.leavittmgt.com> Hi Histonetters! We are looking for TWO full time licensed histotechs here in our very busy Delray Beach, Florida dermatology laboratory. These are permanent full time, (40 hours) position with benefits (medical/401k/vacation). Annual salary in the $50k range (NIGHT SHIFT DIFF AVAILABLE). THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! DAYTIME POSTION: *full time position Mon-Fri 8a-5:30p (start time may vary) *MUST be licensed as a FLORIDA HISTOTECHNICIAN OR HISTOTECHNOLOGIST (NO pending licensure pls) *PREFER experience but WILL TRAIN a knowledgeable, willing candidate *duties to include (but not limited to): grossing, microtomy, accessioning, embedding and general histology *must be self motivated, reliable and a team player DAYTIME SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY: https://advancedderm.applicantpro.com/jobs/240090.html NIGHT POSITION: *Full time position Mon-Fri 10:30p-7:00a (IMMUNOHISTOCHEMISTRY/SPECIALS department) *MUST be licensed as a FLORIDA HISTOTECHNOLOGIST (this is NOT negotiable) *EXPERIENCE WITH IHC A MUST! Leica (BOND) and Roche/Ventana equipment experience preferred *Must be able to multi-task special stains *MUST have at LEAST 2 years experience. Please DO NOT respond if you do not possess EXPERIENCE in this area! *must be confidant, quick learner, self motivated, reliable and a team player NIGHT SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY: https://advancedderm.applicantpro.com/jobs/278020.html Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From Lori.Amos at vdh.virginia.gov Wed Oct 21 13:53:44 2015 From: Lori.Amos at vdh.virginia.gov (Amos, Lori (VDH)) Date: Wed, 21 Oct 2015 18:53:44 +0000 Subject: [Histonet] (no subject) Message-ID: <34AAD3B59AC6474EA3406AEC60C1F89B26A332E4@COVMSGCES-MBX12> I have a beyond repair tissue processor that needs to be dispose of. It has not been in use for a year. All the solutions have been emptied but the paraffin has solidified in the chambers. We found a recycling company that is willing to remove it for us but they are requiring us to first prove that it is not biohazardous. How does one dispose of their tissue processor? Lori From Ronald.Houston at nationwidechildrens.org Wed Oct 21 15:43:11 2015 From: Ronald.Houston at nationwidechildrens.org (Houston, Ronald) Date: Wed, 21 Oct 2015 20:43:11 +0000 Subject: [Histonet] MAP2K7 IHC Message-ID: Does anyone do MAP2K7 immunohistochemistry testing with interpretation on clinical specimens? We have a neuroblastoma that our oncologists want tested. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston at nationwidechildrens.org www.NationwideChildrens.org "Without continual growth and progress, such words as improvement, achievement, and success have no meaning." ~ Ben Franklin From bhartologist at gmail.com Wed Oct 21 17:36:31 2015 From: bhartologist at gmail.com (Bharti Parihar) Date: Wed, 21 Oct 2015 15:36:31 -0700 Subject: [Histonet] Xylene and Formalin substitutes Message-ID: Hello everyone. I'm interested in starting a conversation at my workplace regarding substitutes for xylene, and if there are any substitutes for formalin. If anyone out there is using any can they please give me feedback regarding the following questions, 1. Name of substitutes? 2. Pros and Cons? 3. Any quality issues when performing H&E, IHC or Special Stains? 4. Is it cost effective? 5. Does it require different dehydrants other than alcohol? 6. If formalin is still used as fixative, what xylene substitute works best with formalin? I appreciate any feedback! Thanks! From wdesalvo.cac at outlook.com Wed Oct 21 18:10:49 2015 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Wed, 21 Oct 2015 16:10:49 -0700 Subject: [Histonet] Xylene and Formalin substitutes Message-ID: Contact Ada Feldman, Anatech Ltd. Best resource. Sent from my Windows Phone ________________________________ From: Bharti Parihar via Histonet Sent: ?10/?21/?2015 3:49 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Xylene and Formalin substitutes Hello everyone. I'm interested in starting a conversation at my workplace regarding substitutes for xylene, and if there are any substitutes for formalin. If anyone out there is using any can they please give me feedback regarding the following questions, 1. Name of substitutes? 2. Pros and Cons? 3. Any quality issues when performing H&E, IHC or Special Stains? 4. Is it cost effective? 5. Does it require different dehydrants other than alcohol? 6. If formalin is still used as fixative, what xylene substitute works best with formalin? I appreciate any feedback! Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From areichard at lmhealth.org Thu Oct 22 06:12:19 2015 From: areichard at lmhealth.org (Amanda Reichard) Date: Thu, 22 Oct 2015 07:12:19 -0400 Subject: [Histonet] Xylene and Formalin substitutes In-Reply-To: References: Message-ID: Good Morning! Our lab actually just went through some research regarding this, so I thought I would share. We have used the xylene substitute Americlear from Cardinal Health for a long time. It works well with our reagent alcohols and formalin, and we haven't had any processing or staining issues with it. I think the price isn't that bad either. Another benefit is how safe it is compared to xylene and some other substitutes. We were looking into getting a new piece of cytology equipment, but we would have had to change our xylene substitute. The approved substitutes (Pro-Par, Thermo Shandon Xylene Substitute, and Histo-clear) all had really bad MSDS's. Some of them seemed worse than xylene! I can't remember which one, but it said it caused birth defects which was a big NO since a few of us in the lab still want more babies. Because of the safety issue, we didn't switch. I would be interested in any answers you get for formalin substitutes if you don't mind! I know our lab tried some before, but it didn't fix lymph nodes very well. Have a great Thursday everyone! Amanda Reichard, HTL (ASCP)cm Histology Supervisor Laboratory Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (740) 348-4163 ________________________________________ From: Bharti Parihar via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, October 21, 2015 6:36 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Xylene and Formalin substitutes Hello everyone. I'm interested in starting a conversation at my workplace regarding substitutes for xylene, and if there are any substitutes for formalin. If anyone out there is using any can they please give me feedback regarding the following questions, 1. Name of substitutes? 2. Pros and Cons? 3. Any quality issues when performing H&E, IHC or Special Stains? 4. Is it cost effective? 5. Does it require different dehydrants other than alcohol? 6. If formalin is still used as fixative, what xylene substitute works best with formalin? I appreciate any feedback! Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From rjbuesa at yahoo.com Thu Oct 22 08:02:47 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 22 Oct 2015 13:02:47 +0000 (UTC) Subject: [Histonet] Xylene and Formalin substitutes In-Reply-To: References: Message-ID: <8627805.1646665.1445518967750.JavaMail.yahoo@mail.yahoo.com> The most straight forward solution to your problem is the following: 1- change from ethanol dehydration to 2-propanol (isopropyl-alcohol)2- after 100% propanol, go directly a mixture 1:1 of propanol with mineral oil light molecular weight3- go to your regular paraffin embedding steps. These changes will eliminate xylene and is your best (and cheapest) option because there is NO xylene substitute available that will outperform what I have just recommended and they are ALL very expensive. As regarding formalin, there is NO substitute that will provide a comparable fixation, NO one, no matter what you have "heard" about it.The best option is to reduce the amount of formalin and the exposure.Contrary to "common knowledge" that you need a proportion of 10:1 ? 40:1 of formalin volume to specimen volume, that is an "urban legend" and totally wrong rooted in years of repeating the same thing endlessly.You only need a 2:1 and at the most 5:1 volume of formalin to specimen volume.If you want to have additional information on these two issues and read about it, please visit:Histosearch: The Histology Search Engine | ? | | ? | ? | ? | ? | ? | | Histosearch: The Histology Search EngineCopyright ? 2007. All rights reserved.? Privacy Policy - Disclaimer | | | | View on www.histosearch.com | Preview by Yahoo | | | | ? | Ren? On Wednesday, October 21, 2015 6:55 PM, Bharti Parihar via Histonet wrote: Hello everyone. I'm interested in starting a conversation at my workplace regarding substitutes for xylene,? and if there are any substitutes for formalin. If anyone out there is using any can they please give me feedback regarding the following questions, 1. Name of substitutes? 2. Pros and Cons? 3. Any quality issues when performing H&E, IHC or Special Stains? 4. Is it cost effective? 5. Does it require different dehydrants other than alcohol? 6. If formalin is still used as fixative,? what xylene substitute works best with formalin? I appreciate any feedback! Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas at biopath.org Thu Oct 22 12:26:28 2015 From: plucas at biopath.org (Paula) Date: Thu, 22 Oct 2015 10:26:28 -0700 Subject: [Histonet] Job Opportunities/Orange County, California Message-ID: <000f01d10cee$ca5d7fd0$5f187f70$@biopath.org> Hello, If you or if you know someone who might be interested in the following jobs, please inquire to Russell Blayney at 714-433-1330: 1. Transcriptionist- Full- time 2. Medical Biller- Full-time 3. Laboratory Courier- Part-time morning hours Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA 92708 714-433-1330 From SJMcCabe at phhealthcare.org Thu Oct 22 12:59:50 2015 From: SJMcCabe at phhealthcare.org (McCabe, Sara) Date: Thu, 22 Oct 2015 13:59:50 -0400 Subject: [Histonet] CMV controls Message-ID: <02AE2390303AAB43A823930EAD6B63AE03A4BA3B9F@ex07> Hello all, I am looking for positive CMV controls for use for IHC. If anyone has any that they would be willing to share or trade for other tissue control material please message me! Also, if not, where are you buying your CMV control slides/material from? Thank you in advance! Sara J. McCabe, HT(ASCP)CM Senior Histotechnologist Penn Highlands DuBois 100 Hospital Avenue DuBois, PA 15801 814-375-3264 Telephone 814-375-3784 Fax sjmccabe at phhealthcare.org www.phhealthcare.org [https://exfront.drmc.org/owa/attachment.ashx?id=RgAAAADdnNAtKTbeQZREeHpdjjNJBwBvOqSeu94TQqAsex%2bLq8hQAAARmxV2AAACriOQMDqrQ6gjkw6ta2OuAAECi368AAAJ&attcnt=1&attid0=EABfEFZIze0hTp%2bt8x4s9h42] This email and any attached files are sensitive in nature and intended solely for the intended recipient(s). If you are not the named recipient you should not read, distribute, copy or alter this email. Any views or opinions expressed in this email are those of the author and do not represent those of Penn Highlands Healthcare or its affiliates.. Warning: Although precautions have been taken to make sure no viruses are present in this email, the company cannot accept responsibility for any loss or damage that arise from the use of this email or attachments. From adhall at ael.com Thu Oct 22 13:57:35 2015 From: adhall at ael.com (Angela Hall) Date: Thu, 22 Oct 2015 13:57:35 -0500 Subject: [Histonet] Xylene and Formalin substitutes In-Reply-To: References: Message-ID: Hey, The previous supervisor switched the Histo lab to CBG's Formula 83 with no problems as our xylene substitute. In fact, we even distil it and use the distilled in both our staining and processor. The only con is that it is not as effective in removing coverslips from slides. Unfortunately, I'm not sure about cost effective but our lab tries to balance cost-effective with people-friendly. The cyto lab uses Shandon's due to special requirements on their equipment with no problems. "https://www.cbgtechnologies.com/hap-free-solvents.aspx" Angela D. Hall, BA, HT(ASCP)CM Histology Department American Esoteric Laboratories www.ael-east.com ________________________________ Tel +423 586 3240 ext 1019 or 1041 Fax +423 714 2001 ? This message and any files transmitted with it may contain privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message, you must not disseminate, copy or take any action in reliance on it. If you have received this message in error, please notify?the?sender ?immediately. -----Original Message----- From: Bharti Parihar [mailto:bhartologist at gmail.com] Sent: Wednesday, October 21, 2015 6:37 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Xylene and Formalin substitutes Hello everyone. I'm interested in starting a conversation at my workplace regarding substitutes for xylene, and if there are any substitutes for formalin. If anyone out there is using any can they please give me feedback regarding the following questions, 1. Name of substitutes? 2. Pros and Cons? 3. Any quality issues when performing H&E, IHC or Special Stains? 4. Is it cost effective? 5. Does it require different dehydrants other than alcohol? 6. If formalin is still used as fixative, what xylene substitute works best with formalin? I appreciate any feedback! Thanks! From jaylundgren at gmail.com Thu Oct 22 16:01:13 2015 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 22 Oct 2015 16:01:13 -0500 Subject: [Histonet] Running a histo lab without a histotech? Message-ID: Hello histonetters! I am on assignment in a small hospital lab in the Heartland and they are staffed by only two people. One is a registered (ASCP) HT with 10 years of experience, and the other is a former phlebotomist being trained on the job from scratch , who has yet to take her accreditation exam. As a matter of fact, she has yet to take all the college courses (math, science) to qualify to take her exam, and is still being trained in the hospital's protocols, only allowed to cut larger specimens (no bxs), etc. She can accession, answer the phone, embed, load the stainer, coverslipper, etc., so she can turn out most of the work. I am here working for three weeks because the trainee is on medical leave. My question is, when the registered HT goes on vacation or sick leave, is it legal for the lab to operate with only the trainee? Or does a histo lab have to have at least one registered tech to operate? Thanks, Jay A. Lundgren, M.S., HTL (ASCP) From Natacha.Mitchell at harrishealth.org Thu Oct 22 16:22:03 2015 From: Natacha.Mitchell at harrishealth.org (Mitchell, Natacha A) Date: Thu, 22 Oct 2015 21:22:03 +0000 Subject: [Histonet] Protocols with unstained slides Message-ID: <6441A38AD60C3543B5861E7E678D0ABFBC2B5A@hhmsg02.hchd.local> Hello Fellow Histonetters, We are currently in the process of revising our protocols to reduce the amount of unstained slides cut. Currently, we cut unstains for prostate bx's, (2 H/E, 1 UNS), liver bx's (2 H/E's, 6 UNS), bone marrows (2 H/E's, 6 UNS) and cytology blocks (2 H/E, 8 UNS). I do realize this may be moderate in comparison to other labs where 30 slides are cut for a kidney block. YIKES!! However, in an effort to decrease the amount of unstained slides that are cut and discarded (we do not file unstained slides due to storage constraints) and archive more tissue in paraffin blocks, I am deferring to you all for input. I was curious as to other lab's protocols for these specimen. What are protocols for types of cytology blocks (FNA vs cell block; FNA of thyroid vs FNA of pancreas)? I do understand if specimen is almost nonexistent , it is best to pick up as much as you can because upon recutting you may lose what is left and that upon recutting you run the risk, sometimes, of lesion or positive cells being cut away. It's definitely not an exact science. Your feedback is greatly appreciated. Have a wonderful Friday eve!! Natacha Mitchell, BS, HTL (ASCP) Histology Lab Supervisor Harris Health System Ben Taub General Hospital 1504 Taub Loop Houston, Tx, 77030 Office 713-873-0750 Lab 713-873-3258 Fax 713-873-3214 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From samjr2 at u.washington.edu Thu Oct 22 17:49:53 2015 From: samjr2 at u.washington.edu (Sammie) Date: Thu, 22 Oct 2015 15:49:53 -0700 (PDT) Subject: [Histonet] DR1 and DAT Message-ID: Would someone be willing to share their protocol for DR1 and DAT for human FFPE tissue? Anti-D-1 Dopamine Receptor rat monoclonal from Sigma Anti-Dopamine Transporter rat monoclonal from Millipore and Abcam I have tried a variety of dilutions and retrieval times via our BOND MAX (im trying to avoid doing it by hand) with no luck. Any advice would be appreciated Thanks Sammie University of Washington NeuroHistology - Pathology From rjbuesa at yahoo.com Fri Oct 23 07:35:34 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 23 Oct 2015 12:35:34 +0000 (UTC) Subject: [Histonet] Running a histo lab without a histotech? In-Reply-To: References: Message-ID: <95505590.2321551.1445603734580.JavaMail.yahoo@mail.yahoo.com> About the "legality" I do not think it is of your direct concern, unless you want to "challenge" the situation with some sort of legal action, which is really not advisable.As a functioning laboratory in a hospital it has to be under the supervision of either CLIA or? ASAP and the pathologist is the Director of such laboratory so, it is the pathologist's responsibility of assuming about the "legality" of that situation and is the pathology who you should ask this question.Ren? On Thursday, October 22, 2015 5:02 PM, Jay Lundgren via Histonet wrote: Hello histonetters!? I am on assignment in a small hospital lab in the Heartland and they are staffed by only two people.? One is a registered (ASCP) HT with 10 years of experience, and the other is a former phlebotomist being trained on the job from scratch , who has yet to take her accreditation exam. As a matter of fact, she has yet to take all the college courses (math, science) to qualify to take her exam, and is still being? trained in the hospital's protocols, only allowed to cut larger specimens (no bxs), etc. She can accession, answer the phone, embed, load the stainer, coverslipper, etc.,? so she can turn out most of the work.? I am here working for three weeks because the trainee is on medical leave. My question is, when the registered HT goes on vacation or sick leave, is it legal for the lab to operate with only the trainee? Or does a histo lab have to have at least one registered tech to operate? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Thanks, ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree at uwhealth.org Fri Oct 23 09:16:58 2015 From: LSebree at uwhealth.org (Sebree Linda A) Date: Fri, 23 Oct 2015 14:16:58 +0000 Subject: [Histonet] Reference lab offering ARIDIA on FFPE tissue? Message-ID: <77DD817201982748BC67D7960F2F76AF186336@UWHC-MBX12.uwhis.hosp.wisc.edu> Hello Histonetters, Anyone know of a reference lab offering this antibody? Thanks, Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From LSebree at uwhealth.org Fri Oct 23 11:06:00 2015 From: LSebree at uwhealth.org (Sebree Linda A) Date: Fri, 23 Oct 2015 16:06:00 +0000 Subject: [Histonet] Reference lab offering ARID-1a on FFPE tissue? Message-ID: <77DD817201982748BC67D7960F2F76AF187398@UWHC-MBX12.uwhis.hosp.wisc.edu> Sorry, I meant ARID-1a. From: Sebree Linda A Sent: Friday, October 23, 2015 9:17 AM To: Histonet (Histonet at lists.utsouthwestern.edu) Cc: Weisman Paul Subject: Reference lab offering ARIDIA on FFPE tissue? Hello Histonetters, Anyone know of a reference lab offering this antibody? Thanks, Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From CIngles at uwhealth.org Fri Oct 23 12:09:09 2015 From: CIngles at uwhealth.org (Ingles Claire) Date: Fri, 23 Oct 2015 17:09:09 +0000 Subject: [Histonet] Running a histo lab without a histotech? In-Reply-To: <95505590.2321551.1445603734580.JavaMail.yahoo@mail.yahoo.com> References: , <95505590.2321551.1445603734580.JavaMail.yahoo@mail.yahoo.com> Message-ID: Not to my knowledge. We have 3 derm/Mohs labs here in our system. There are only two of us who have our certifications. Heaven knows the world (or the lab for that matter) doesn't stop revolving when WE go on vacation. The third lab is a one person show and she isn't certified. Just so all the CLIA/JCHO/CAP stuff is being adhered to. The only thing I can think of regarding training/college must haves are for grossing as it is considered high complexity. I agree with Rene. Not your problem. Claire ________________________________________ From: Rene J Buesa via Histonet [histonet at lists.utsouthwestern.edu] Sent: Friday, October 23, 2015 7:35 AM To: Jay Lundgren; histonet Subject: Re: [Histonet] Running a histo lab without a histotech? About the "legality" I do not think it is of your direct concern, unless you want to "challenge" the situation with some sort of legal action, which is really not advisable.As a functioning laboratory in a hospital it has to be under the supervision of either CLIA or ASAP and the pathologist is the Director of such laboratory so, it is the pathologist's responsibility of assuming about the "legality" of that situation and is the pathology who you should ask this question.Ren? On Thursday, October 22, 2015 5:02 PM, Jay Lundgren via Histonet wrote: Hello histonetters! I am on assignment in a small hospital lab in the Heartland and they are staffed by only two people. One is a registered (ASCP) HT with 10 years of experience, and the other is a former phlebotomist being trained on the job from scratch , who has yet to take her accreditation exam. As a matter of fact, she has yet to take all the college courses (math, science) to qualify to take her exam, and is still being trained in the hospital's protocols, only allowed to cut larger specimens (no bxs), etc. She can accession, answer the phone, embed, load the stainer, coverslipper, etc., so she can turn out most of the work. I am here working for three weeks because the trainee is on medical leave. My question is, when the registered HT goes on vacation or sick leave, is it legal for the lab to operate with only the trainee? Or does a histo lab have to have at least one registered tech to operate? Thanks, Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree at uwhealth.org Fri Oct 23 14:25:53 2015 From: LSebree at uwhealth.org (Sebree Linda A) Date: Fri, 23 Oct 2015 19:25:53 +0000 Subject: [Histonet] Reference lab offering BRAF by IHC on FFPE specimens Message-ID: <77DD817201982748BC67D7960F2F76AF1873F7@UWHC-MBX12.uwhis.hosp.wisc.edu> We're looking for a reference lab that performs the above immunohistochemical stain. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From garreyf at gmail.com Sat Oct 24 07:32:34 2015 From: garreyf at gmail.com (Garreyf) Date: Sat, 24 Oct 2015 08:32:34 -0400 Subject: [Histonet] Running a histo lab without a histotech? In-Reply-To: References: <95505590.2321551.1445603734580.JavaMail.yahoo@mail.yahoo.com> Message-ID: What I find really weird and hard to understand is: That person who is unable (CLIA-wise) to gross the small biopsy because of inadequate science training etc, is able to embed, cut and stain that same biopsy. The histology part is so much harder and requires much more training and expertise. It just doesn't make any sense to me. I'm not arguing that all histotechs need this science training, I'd argue that the clia rule for grossing smalls is too stringent. Thanks my 2 cents. Garrey Sent from my iPhone > On Oct 23, 2015, at 1:09 PM, Ingles Claire via Histonet wrote: > > Not to my knowledge. We have 3 derm/Mohs labs here in our system. There are only two of us who have our certifications. Heaven knows the world (or the lab for that matter) doesn't stop revolving when WE go on vacation. The third lab is a one person show and she isn't certified. Just so all the CLIA/JCHO/CAP stuff is being adhered to. The only thing I can think of regarding training/college must haves are for grossing as it is considered high complexity. I agree with Rene. Not your problem. > Claire > > ________________________________________ > From: Rene J Buesa via Histonet [histonet at lists.utsouthwestern.edu] > Sent: Friday, October 23, 2015 7:35 AM > To: Jay Lundgren; histonet > Subject: Re: [Histonet] Running a histo lab without a histotech? > > About the "legality" I do not think it is of your direct concern, unless you want to "challenge" the situation with some sort of legal action, which is really not advisable.As a functioning laboratory in a hospital it has to be under the supervision of either CLIA or ASAP and the pathologist is the Director of such laboratory so, it is the pathologist's responsibility of assuming about the "legality" of that situation and is the pathology who you should ask this question.Ren? > > > On Thursday, October 22, 2015 5:02 PM, Jay Lundgren via Histonet wrote: > > > Hello histonetters! I am on assignment in a small hospital lab in the > Heartland and they are staffed by only two people. One is a registered > (ASCP) HT with 10 years of experience, and the other is a former > phlebotomist being trained on the job from scratch , who has yet to take > her accreditation exam. > > As a matter of fact, she has yet to take all the college courses (math, > science) to qualify to take her exam, and is still being trained in the > hospital's protocols, only allowed to cut larger specimens (no bxs), etc. > She can accession, answer the phone, embed, load the stainer, coverslipper, > etc., so she can turn out most of the work. I am here working for three > weeks because the trainee is on medical leave. > > My question is, when the registered HT goes on vacation or sick leave, is > it legal for the lab to operate with only the trainee? > > Or does a histo lab have to have at least one registered tech to operate? > > > Thanks, > > Jay A. Lundgren, M.S., HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From taylor at prometheushealthcare.com Mon Oct 26 09:04:15 2015 From: taylor at prometheushealthcare.com (Taylor Rinaldi) Date: Mon, 26 Oct 2015 10:04:15 -0400 Subject: [Histonet] **CALIFORNIA** Histotechnician and Supervisor Opportunities Message-ID: <075e01d10ff7$3368a5d0$9a39f170$@prometheushealthcare.com> Hello all! My name is Taylor Rinaldi, Recruiting Manager at Prometheus healthcare. We specialize specifically in laboratory recruiting all over the United States for many hospitals and reference laboratories. We just received a new order for a great opportunity with a well-known laboratory located in California. We are recruiting for a strong Histotechnician and a Histology Supervisor for the lab. These positions are fulltime, permanent opportunities. ASCP certification preferred. Relocation assistance offered. If you may be interested in this opportunity or would like to hear about our nationwide histotech openings, please reach out to me for immediate consideration. Thank you in advance! Taylor Rinaldi Recruiting Manager Prometheus Healthcare Office (301) 693-9057 Taylor at prometheushealthcare.com From relia1 at earthlink.net Mon Oct 26 09:26:17 2015 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 26 Oct 2015 10:26:17 -0400 Subject: [Histonet] RELIA HOT JOB Alert!! Exciting opportunities in CA. Los Angeles Area and San Francisco Bay area. Histotechs and Supervisor needed! Message-ID: <013f01d10ffa$47ce0620$d76a1260$@earthlink.net> Hi Histonetters!! I hope this is the start of a GREAT week for you. My phone is ALREADY ringing off the hook with exciting new opportunities. I wanted to post these positions right away because my clients are offering excellent pay, benefits, relocation assistance and sign on bonuses. I know I am not the only recruiter out there contacting you but I am the ONLY recruiter specializing exclusively in the permanent placement of histology professionals. I have been doing so for over 10 years. I know my clients and I take the time to talk to you about what you want. Here is the information on the California positions: Los Angeles Area: Histotech with strong IHC days full time permanent San Francisco Bay Area: Histology Manager Histology Tech If you are interested in more information on any of these opportunities please contact me right away. I can be reached toll free at 866-607-3542, via email at relia1 at earthlink.net or on my cell call/text to 407-353-5070. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From robrankin at rankinbiomed.com Mon Oct 26 11:56:06 2015 From: robrankin at rankinbiomed.com (Rob Rankin) Date: Mon, 26 Oct 2015 12:56:06 -0400 Subject: [Histonet] Please post Message-ID: Hi Histonetters! We are in need of used Vibratomes for research facilities. Models we will buy are the 1000 Plus and 1500, working or broken units. Contact Amy at amy at rankinbiomed.com or 248 625-4104. Respectfully, *Rob Rankin, MSM, SM(ASCP)* Founder & CEO 248.625.4104 *RANKIN* 14515 Mackey Road Holly, MI 48442 www.rankinbiomed.com facebook.com/rankinbiomed twitter.com/rankinbiomed.com From histology81176 at att.net Tue Oct 27 08:17:32 2015 From: histology81176 at att.net (Histology Technician) Date: Tue, 27 Oct 2015 13:17:32 +0000 (UTC) Subject: [Histonet] Please post In-Reply-To: References: Message-ID: <684735418.197972.1445951852710.JavaMail.yahoo@mail.yahoo.com> Co-path users...how do you assign cases to your Pathologists?? I have 3 Paths and the senior Path comes in early and divides the cases up himself, but starting in Jan we are going to start assigning cases to each path when we accession a case.? Any thoughts on how to do this smoothly? Thanks! On Monday, October 26, 2015 11:57 AM, Rob Rankin via Histonet wrote: Hi Histonetters! We are in need of used Vibratomes for research facilities. Models we will buy are the 1000 Plus and 1500, working or broken units. Contact Amy at amy at rankinbiomed.com or 248 625-4104. Respectfully, *Rob Rankin, MSM, SM(ASCP)* Founder & CEO 248.625.4104 *RANKIN* 14515 Mackey Road Holly, MI 48442 www.rankinbiomed.com facebook.com/rankinbiomed twitter.com/rankinbiomed.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From oneilb at wvuhealthcare.com Tue Oct 27 09:29:48 2015 From: oneilb at wvuhealthcare.com (O'neil, Beth) Date: Tue, 27 Oct 2015 14:29:48 +0000 Subject: [Histonet] Anti-nitrotyrosine Message-ID: <3CEB8EBCF9C7A648B9694B5696462A7172680809@NT-EX1.wvuhs.com> Would anyone have automated IHC staining protocols for Anti-nitrotyrosine? The antibody is from Santa Cruz Biotechnology #55256. Any help would be appreciated. Thanks Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC oneilb at wvuhealthcare.com Histology Supervisor, Technical Specialist Lab: 304 - 293 - 6014 Office: 304 - 293 - 7629 ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From relia1 at earthlink.net Tue Oct 27 10:55:09 2015 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 27 Oct 2015 11:55:09 -0400 Subject: [Histonet] Happy Halloween 2015 Here's a fun Halloween Hack and a Trick or Treat message from RELIA! Message-ID: <001c01d110cf$dbb25ff0$93171fd0$@earthlink.net> Halloween is just around the corner. Here's a fun Halloween Hack. Make your beverages glow by adding a crushed B2 vitamin. Here is the link with directions: http://ladyfordsguidetoeverything.blogspot.co.uk/2013/10/amazing.html HAPPY HALLOWEEN!!! TRICK or TREAT!!!!! The TRICK is finding the right job opportunity for you. The TREAT is with my help it can be relatively painless. . I will help you with your resume, . Coach you through the interview and offer process . And refer you to positions based on the criteria you give me. . I will never give your resume to an employer without your permission. I know the "OTHER" recruiters can't make that promise! All of the positions I work with are fulltime permanent positions with some of the best facilities Nationwide. My clients offer excellent compensation including competitive salaries, great benefits, relocation assistance/sign on bonuses. Here is a list of my current openings: My NEWEST AND MOST EXCITING OPPORTUNITIES: Histology Manager - Modesto, CA Exciting oppty with a great team Histology Tech - Modesto, CA Great place to use your IHC expertise! Senior Histotech - Norfolk, VA 15 Sign on BS required. Histotech - Ann Arbor, MI evenings generous shift diff! Dermpath Histotech - Denver, CO Run your own lab!! IHC Specialist - Los Angeles,CA NO BENCH WORK! Histotech - Charlotte, NC Nights, Dermpath Histotech - Longview, TX Histotech - Atlanta, GA Dermpath Histotech - Fayetteville, AR If you or any of your friends would like more information on any of these positions or if you would like to discuss opportunities in other areas or future job searches please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1 at earthlink.net Or on my cell call/text 407-353-5070. Remember I offer over 20 years of recruiting and employment counseling experience, knowledgeable, confidential and responsive service to you and your friends and a permanent placement practice dedicated to the histology profession. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember. It never hurts to keep an eye open even if you are happy in your present job. Happy Halloween!!!!!!!!!!!! Pam - 866-607-3542 (866-60RELIA) p.s. What are you going to be for Halloween? Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From vavalos at allergydermatology.com Tue Oct 27 11:07:29 2015 From: vavalos at allergydermatology.com (Vanessa Avalos) Date: Tue, 27 Oct 2015 16:07:29 +0000 Subject: [Histonet] MOHS TECH NEEDED - ARIZONA Message-ID: MOHS TECH - IMMEDIATE OPENING - ARIZONA Derm office in immediate need of an experienced F/T MOHS TECH. Will work with one Mohs Surgeon and must have dependable transportation to alternate offices daily. Offices are in Surprise and Glendale, approximately 11 miles apart. If you or anyone you know is interested please fax resume to # below or respond to this listing. ***** This is not a job agency. I have been employed with the practice for over 15 years. ***** Thank You! V.Avalos ADS, INC Fax:602-277-2134 From PKRichar at gundersenhealth.org Tue Oct 27 11:31:45 2015 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Tue, 27 Oct 2015 16:31:45 +0000 Subject: [Histonet] Workstations Message-ID: <998284C32F61104CA0BEFFFFCF6F90FD6CFE30CC@LXEXMB01.gundluth.org> Hi, We currently have one large table that six histology techs work around. We are looking into getting individual workstations to be more ergonomic. Can you share what type of workstations you have and the size? Cordially, Pam ~ +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org From a_schade at conradweiser.org Tue Oct 27 11:48:53 2015 From: a_schade at conradweiser.org (Schade, Adelle) Date: Tue, 27 Oct 2015 16:48:53 +0000 Subject: [Histonet] olympus dp70 microscope camera Message-ID: Hello, I developed a histology lab in my high school and wanted to say THANK YOU to all of the individuals on this list that supported, donated and offered consultation through the process. The students love the hands on approach to cell and tissue study. The lab is full of students through the school day...and they keep asking for more time after school and weekends as well. The "histology" community has truly been an amazing support system- very special people! I received an Olympus DP70 digital microscope camera as a donation, which will be a great addition to the lab for imaging. We do not have the budget to buy new cameras. I have the camera and the cable, however, I am searching for the PCI board and the software. I contacted my Olympus rep and some local contacts to put the word out. If anyone on this listserv has information on the PCI board and software for this, I would appreciate the information. Please contact me. a_schade at conradweiser.org Thanks again for your help! Have a great day, Adelle Ms. Adelle L. Schade, B.S., M.Ed., M.S. Biomedical Science Conrad Weiser High School 44 Big Spring Rd. Robesonia, PA 19551 610-693-8599 x6783 a_schade at conradweiser.org From histology81176 at att.net Tue Oct 27 13:19:09 2015 From: histology81176 at att.net (Histology Technician) Date: Tue, 27 Oct 2015 18:19:09 +0000 (UTC) Subject: [Histonet] Gross Room QC References: <1520136711.409614.1445969949376.JavaMail.yahoo@mail.yahoo.com> Message-ID: <1520136711.409614.1445969949376.JavaMail.yahoo@mail.yahoo.com> I just made up a QC sheet for my Gross Room and I'm having a hard time finding a spot to keep it :)? Do I tape it on the wall by the Grossing Station or put a magnet on it attaching to the grossing table... Any ideas? Thanks! From Donna.Willis at baylorhealth.edu Tue Oct 27 13:58:48 2015 From: Donna.Willis at baylorhealth.edu (Willis, Donna G.) Date: Tue, 27 Oct 2015 18:58:48 +0000 Subject: [Histonet] Baylor University Medical Center Histology Supervisor Message-ID: <2572B4D63B62E64A8078D8BBE34D40788DBF5B1C@BHDASVEXML2.bhcs.pvt> Good afternoon everyone. I have a new position that I would like to mention. Hope to get some good applicants. Supervisor, Histology - Dallas, Texas - Baylor Scott and White Health Requisition Number: 193596_1 The Histology Lab Supervisor oversees/supervises the routine activities of the laboratory or laboratory section to ensure that staffing is adequate and that tests are performed, recorded, and reported in a timely manner and in accordance with quality standards. The Histology lab Supervisor assists the manager as defined by the laboratory or laboratory section policy, with staff hiring, training, performance evaluations, payroll, etc. Requirements: HT or HTL (License/Certification) Required 2 Years of Experience Associated with Progressive Responsibility College Degree- Bachelor of Science If you are qualified and interested in applying to this position, please go to: http://jobs.baylorscottandwhite.com/ and enter the job requisition number 193596_1 in the keyword search. Thanks to all, Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From bpaniccia.5 at gmail.com Tue Oct 27 15:55:41 2015 From: bpaniccia.5 at gmail.com (Bernard Paniccia) Date: Tue, 27 Oct 2015 16:55:41 -0400 Subject: [Histonet] Hematoxylin streaking Message-ID: Hello histonet! Long time, first time. My lab recently transitioned from hand staining for our routine H+Es to using the Leica autostainer XL. The machine seems to work fine and the protocol is nearly identical to our hand staining one. The problem we've run into is that there is a streak of residual hematoxylin remaining on each slide. Plus slides are worse than non-plus slides. I've tried it with Richard-Allen Gill Hematoxylin and Surgipath Selectech Hematoxylin 560 MX and the streaks are still there. Note the streaking doesn't occur when I hand rinse the racks directly under sink water rather than in the wash bins of the autostainer. Also we use a regressive stain. Has anyone seen something like this before? Any trouble shooting tips? Is it possible the water pressure of the in-machine rinse is too low? We want the slides to come out crisp and clear without the streaking purple halo down the middle. Thanks so much! -Bernard From pdefazio802 at gmail.com Tue Oct 27 17:07:25 2015 From: pdefazio802 at gmail.com (Pam DeFazio) Date: Tue, 27 Oct 2015 18:07:25 -0400 Subject: [Histonet] Histonet Digest, Vol 143, Issue 23 In-Reply-To: References: Message-ID: Re: Assigning cases to pathologist We use an "A B C D" system. "A" pathologist does all the grossing for that day. "B" reads all slides, except endoscopy and outpatient gyn bx's. So whoever is "A" on say Monday will be "B" on Tues. in other works the grossing "A" pathologist reads the majority of what he/she grossed the day before. "C" & "D" read GI's and OP gyn bx's On Tuesday, October 27, 2015, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Please post (Histology Technician) > 2. Anti-nitrotyrosine (O'neil, Beth) > 3. Happy Halloween 2015 Here's a fun Halloween Hack and a Trick > or Treat message from RELIA! (Pam Barker) > 4. MOHS TECH NEEDED - ARIZONA (Vanessa Avalos) > 5. Workstations (Richardson, Pam K) > 6. olympus dp70 microscope camera (Schade, Adelle) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 27 Oct 2015 13:17:32 +0000 (UTC) > From: Histology Technician > > To: "histonet at lists.utsouthwestern.edu " > >, > "histonet-owner at lists.utsouthwestern.edu " > > > Subject: Re: [Histonet] Please post > Message-ID: > <684735418.197972.1445951852710.JavaMail.yahoo at mail.yahoo.com > > > Content-Type: text/plain; charset=UTF-8 > > Co-path users...how do you assign cases to your Pathologists?? I have 3 > Paths and the senior Path comes in early and divides the cases up himself, > but starting in Jan we are going to start assigning cases to each path when > we accession a case.? Any thoughts on how to do this smoothly? > Thanks! > > > On Monday, October 26, 2015 11:57 AM, Rob Rankin via Histonet < > histonet at lists.utsouthwestern.edu > wrote: > > > Hi Histonetters! We are in need of used Vibratomes for research > facilities. > Models we will buy are the 1000 Plus and 1500, working or broken units. > Contact Amy at amy at rankinbiomed.com or 248 625-4104. > > Respectfully, > *Rob Rankin, MSM, SM(ASCP)* > Founder & CEO > 248.625.4104 > > *RANKIN* > 14515 Mackey Road > Holly, MI 48442 > www.rankinbiomed.com > facebook.com/rankinbiomed > twitter.com/rankinbiomed.com > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 2 > Date: Tue, 27 Oct 2015 14:29:48 +0000 > From: "O'neil, Beth" > > To: "histonet at lists.utsouthwestern.edu " > > > Cc: "Robinson, Lisa" > > Subject: [Histonet] Anti-nitrotyrosine > Message-ID: > <3CEB8EBCF9C7A648B9694B5696462A7172680809 at NT-EX1.wvuhs.com > > > Content-Type: text/plain; charset="us-ascii" > > Would anyone have automated IHC staining protocols for > Anti-nitrotyrosine? The antibody is from Santa Cruz Biotechnology #55256. > Any help would be appreciated. Thanks > > Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC > oneilb at wvuhealthcare.com > > Histology Supervisor, Technical Specialist > Lab: 304 - 293 - 6014 > Office: 304 - 293 - 7629 > > > > ----------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > > > ------------------------------ > > Message: 3 > Date: Tue, 27 Oct 2015 11:55:09 -0400 > From: "Pam Barker" > > To: "Histonet" > > Subject: [Histonet] Happy Halloween 2015 Here's a fun Halloween Hack > and a Trick or Treat message from RELIA! > Message-ID: <001c01d110cf$dbb25ff0$93171fd0$@earthlink.net> > Content-Type: text/plain; charset="us-ascii" > > > Halloween is just around the corner. > Here's a fun Halloween Hack. > Make your beverages glow by adding a crushed B2 vitamin. > Here is the link with directions: > http://ladyfordsguidetoeverything.blogspot.co.uk/2013/10/amazing.html > > HAPPY HALLOWEEN!!! > > TRICK or TREAT!!!!! > The TRICK is finding the right job opportunity for you. > The TREAT is with my help it can be relatively painless. > . I will help you with your resume, > . Coach you through the interview and offer process > . And refer you to positions based on the criteria you give me. > . I will never give your resume to an employer without your > permission. > I know the "OTHER" recruiters can't make that promise! > All of the positions I work with are fulltime permanent positions with > some > of the best facilities Nationwide. My clients offer excellent > compensation > including competitive salaries, great benefits, relocation assistance/sign > on bonuses. > Here is a list of my current openings: > My NEWEST AND MOST EXCITING OPPORTUNITIES: > Histology Manager - Modesto, CA Exciting oppty with a great team > Histology Tech - Modesto, CA Great place to use your IHC expertise! > Senior Histotech - Norfolk, VA 15 Sign on BS required. > Histotech - Ann Arbor, MI evenings generous shift diff! > Dermpath Histotech - Denver, CO Run your own lab!! > IHC Specialist - Los Angeles,CA NO BENCH WORK! > Histotech - Charlotte, NC Nights, > Dermpath Histotech - Longview, TX > Histotech - Atlanta, GA > Dermpath Histotech - Fayetteville, AR > If you or any of your friends would like more information on any of these > positions or if you would like to discuss opportunities in other areas or > future job searches please contact me. I would be more than happy to > assist > you. You can reach me at 866-607-3542 or relia1 at earthlink.net > > Or on my cell call/text 407-353-5070. > > Remember I offer over 20 years of recruiting and employment counseling > experience, knowledgeable, confidential and responsive service to you and > your friends and a permanent placement practice dedicated to the histology > profession. > > I work with facilities nationwide and I will keep your resume confidential. > I will only represent you to jobs you tell me you are interested in looking > into. > > Remember. It never hurts to keep an eye open even if you are happy in your > present job. > > Happy Halloween!!!!!!!!!!!! > Pam - 866-607-3542 (866-60RELIA) > > p.s. What are you going to be for Halloween? > > > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > Pam M. Barker > > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1 at earthlink.net > www.facebook.com /PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > > > > > > > > ------------------------------ > > Message: 4 > Date: Tue, 27 Oct 2015 16:07:29 +0000 > From: Vanessa Avalos > > To: "histonet at lists.utsouthwestern.edu " > > > Subject: [Histonet] MOHS TECH NEEDED - ARIZONA > Message-ID: > < > BN1PR07MB871F8576D7E9161DDD61855D4220 at BN1PR07MB871.namprd07.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > MOHS TECH - IMMEDIATE OPENING - ARIZONA > > Derm office in immediate need of an experienced F/T MOHS TECH. Will > work with one Mohs Surgeon and must have dependable transportation to > alternate offices daily. Offices are in Surprise and Glendale, > approximately 11 miles apart. > > If you or anyone you know is interested please fax resume to # below or > respond to this listing. > > ***** This is not a job agency. I have been employed with the practice > for over 15 years. ***** > > Thank You! > > V.Avalos > ADS, INC > Fax:602-277-2134 > > > > ------------------------------ > > Message: 5 > Date: Tue, 27 Oct 2015 16:31:45 +0000 > From: "Richardson, Pam K" > > To: "'histonet at lists.utsouthwestern.edu '" > > > Subject: [Histonet] Workstations > Message-ID: > <998284C32F61104CA0BEFFFFCF6F90FD6CFE30CC at LXEXMB01.gundluth.org > > > Content-Type: text/plain; charset="us-ascii" > > Hi, We currently have one large table that six histology techs work > around. We are looking into getting individual workstations to be more > ergonomic. Can you share what type of workstations you have and the size? > > Cordially, > > Pam ~ > +++++++++++++++++++++++++++ > Pam Richardson > Clinical Manager > Gundersen Health System Laboratory Services > Email: pkrichar at gundersenhealth.org > Phone: 608 775-4133 > Fax: 608 775-6136 > Interdepartmental Mail Stop: H04-007 > E-visit us at: http://www.gundersenhealth.org > > > > ------------------------------ > > Message: 6 > Date: Tue, 27 Oct 2015 16:48:53 +0000 > From: "Schade, Adelle" > > To: "'histonet at lists.utsouthwestern.edu '" > > > Subject: [Histonet] olympus dp70 microscope camera > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Hello, > I developed a histology lab in my high school and wanted to say THANK YOU > to all of the individuals on this list that supported, donated and offered > consultation through the process. The students love the hands on approach > to cell and tissue study. The lab is full of students through the school > day...and they keep asking for more time after school and weekends as > well. The "histology" community has truly been an amazing support system- > very special people! > > I received an Olympus DP70 digital microscope camera as a donation, which > will be a great addition to the lab for imaging. We do not have the budget > to buy new cameras. I have the camera and the cable, however, I am > searching for the PCI board and the software. I contacted my Olympus rep > and some local contacts to put the word out. If anyone on this listserv > has information on the PCI board and software for this, I would appreciate > the information. Please contact me. a_schade at conradweiser.org > > > > Thanks again for your help! > Have a great day, > Adelle > > Ms. Adelle L. Schade, B.S., M.Ed., M.S. > Biomedical Science > Conrad Weiser High School > 44 Big Spring Rd. > Robesonia, PA 19551 > 610-693-8599 x6783 > a_schade at conradweiser.org > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 143, Issue 23 > ***************************************** > From SPINHEIRO at lumc.edu Wed Oct 28 11:33:20 2015 From: SPINHEIRO at lumc.edu (STEVEN PINHEIRO) Date: Wed, 28 Oct 2015 16:33:20 +0000 Subject: [Histonet] Pathology amended reports Message-ID: I realize this is a broad topic but I am trying to get a flavor for best practices. When you issue an amended report, is the original 'removed'? Unlike the practice on the clinical lab side of leaving the first errant test result in place and issuing a corrected result, pathology (at least here) has felt there are more down side issues with leaving the wrong information in the patient chart. Your specific thoughts on the actual protocol used for amended reports issued in your practice is appreciated. Thanks Steven Pinheiro, MBA, MLS(ASCP)DLMCM Manager Anatomic Pathology and Cytology Loyola University Medical Center 2160 S First Ave, Bldg 110 Rm 2214 Maywood, IL 60153 708-327-2642 (O) 708-327-2620 (F) spinheiro at lumc.edu "You must do the thing you think you cannot do" E. Roosevelt Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From tbraud at holyredeemer.com Wed Oct 28 12:46:52 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 28 Oct 2015 17:46:52 +0000 Subject: [Histonet] Gross Room QC Message-ID: <48E053DDF6CE074DB6A7414BA05403F8044A8F@HRHEX02-HOS.holyredeemer.local> All of our QC sheets around the department are on obnoxiously colored fluorescent orange clipboards. I use 3M Command hooks to hang the clipboards near or on the instrument or area. I use a single clear plastic page protector clipped on top of the paper to protect them from splashes, etc. Looks nice, and they are always easy to find and use. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 1. Gross Room QC (Histology Technician) ---------------------------------------------------------------------- Message: 1 Date: Tue, 27 Oct 2015 18:19:09 +0000 (UTC) From: Histology Technician > Subject: [Histonet] Gross Room QC I just made up a QC sheet for my Gross Room and I'm having a hard time finding a spot to keep it :)? Do I tape it on the wall by the Grossing Station or put a magnet on it attaching to the grossing table... Any ideas? Thanks! From victor_tobias at comcast.net Wed Oct 28 12:47:59 2015 From: victor_tobias at comcast.net (Victor) Date: Wed, 28 Oct 2015 10:47:59 -0700 Subject: [Histonet] Pathology amended reports In-Reply-To: References: Message-ID: Steven, Our database keeps track of every variation of the report, but the EMR/clinician only sees the latest copy. We try to limit the use of amendments to demographic changes, date of service, but there are circumstances where an amendment is more appropriate than an addendum. Let me know if you have any questions. Victor University of Washington Medical Center Seattle, WA Sent from Mail for Windows 10 From: STEVEN PINHEIRO via Histonet Sent: Wednesday, October 28, 2015 9:35 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pathology amended reports I realize this is a broad topic but I am trying to get a flavor for best practices. When you issue an amended report, is the original 'removed'? Unlike the practice on the clinical lab side of leaving the first errant test result in place and issuing a corrected result, pathology (at least here) has felt there are more down side issues with leaving the wrong information in the patient chart. Your specific thoughts on the actual protocol used for amended reports issued in your practice is appreciated. Thanks Steven Pinheiro, MBA, MLS(ASCP)DLMCM Manager Anatomic Pathology and Cytology Loyola University Medical Center 2160 S First Ave, Bldg 110 Rm 2214 Maywood, IL 60153 708-327-2642 (O) 708-327-2620 (F) spinheiro at lumc.edu "You must do the thing you think you cannot do" E. Roosevelt Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sforeman at labpath.com Wed Oct 28 13:14:09 2015 From: sforeman at labpath.com (Susan Foreman) Date: Wed, 28 Oct 2015 14:14:09 -0400 Subject: [Histonet] FW: Reference lab offering BRAF by IHC on FFPE specimens In-Reply-To: <77DD817201982748BC67D7960F2F76AF1873F7@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <77DD817201982748BC67D7960F2F76AF1873F7@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: <060f01d111ac$7115db80$53419280$@labpath.com> We run this stain nearly every day with great success. BRAF VE1 V600E. Please contact our laboratory for more information. Susan Foreman, HT (ASCP) KDL Pathology 315 Erin Drive Knoxville, TN 37919 (865)584-1933 -----Original Message----- From: Sebree Linda A via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, October 23, 2015 3:26 PM To: Histonet (Histonet at lists.utsouthwestern.edu) Cc: Yang David T Subject: [Histonet] Reference lab offering BRAF by IHC on FFPE specimens We're looking for a reference lab that performs the above immunohistochemical stain. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sruby at 4path.com Thu Oct 29 10:09:11 2015 From: Sruby at 4path.com (Stephen G. Ruby) Date: Thu, 29 Oct 2015 10:09:11 -0500 Subject: [Histonet] Histonet Digest, Vol 143, Issue 24 In-Reply-To: References: Message-ID: Hey all....I am working on a paper about problems in the histology labs. Can I get you to answer this quick 7 question survey. Nothing of personal nature. Just trying to get a handle on the biggest problems in histology today so I make sure I have addressed as much as possible. Your help is VERY MUCH APPRECIATED. Thanks! Use this link to take the survey: https://docs.google.com/forms/d/12uRzqIn4gAFbI6tf72WCM9CsVg_duKb5PT_Jivi2Wqs/viewform?usp=send_form Stephen G. Ruby, MD, MBA, FCAP President and Medical Director, 4path, Ltd. Value, Service and Commitment...Beyond the Diagnosis V: 1-877-884-7284 F: 630-780-4909 www.4path.com 8238 S. Madison St. Burr Ridge, IL 60527 THIS ELECTRONIC MAIL TRANSMISSION AND ANY ATTACHMENTS MAY CONTAIN PRIVILEGED, CONFIDENTIAL, OR PROPRIETARY INFORMATION INTENDED ONLY FOR THE PERSON(S) NAMED. 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From ASelf at tidelandshealth.org Thu Oct 29 11:29:50 2015 From: ASelf at tidelandshealth.org (Amy Self) Date: Thu, 29 Oct 2015 12:29:50 -0400 Subject: [Histonet] CAP Checklist Question Message-ID: Histonetters, I am going through the CAP checklist and was wondering how the following question below is being handled by everyone that gets inspected by CAP. ANP.12360 Report Completeness Surgical pathology reports for specimens with malignant diagnosis from definitive cancer resections are outlined in the CAP Cancer Protocols are audited annually to ensure that all required elements are included. Thanks in advance for your help, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From emartinez2 at echd.org Thu Oct 29 11:42:48 2015 From: emartinez2 at echd.org (Estela Martinez) Date: Thu, 29 Oct 2015 16:42:48 +0000 Subject: [Histonet] Cost Per Test Message-ID: Hello My Histology Friends, I have been asked to get a cost per test in histology for Level I, II, III, IV, V and VI. Do you guys have a format or can give me some idea how to start this in an easy way. This seems so complicated to me. Thank you so much for your help. Estela Martinez Histology Supervisor Medical Center Health System Odessa, TX 432-640-2348 emartinez2 at echd.org CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party without permission of original user and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From LRaff at uropartners.com Thu Oct 29 12:06:40 2015 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 29 Oct 2015 17:06:40 +0000 Subject: [Histonet] Blog Site Invitation Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B4A99A0@COLOEXCH01.uropartners.local> Hello 'netters As a regular reader and occasional contributor to the list, I wanted to spread the word about a general interest/home building/little bit of pathology blog I have been writing for a few months. It has now joined the Chicago Tribune owned media group ChicagoNow, and all are welcome to take a look. The focus is ever so slightly Chicago, but readers are national and international. Thanks for giving it a spin. Read/comment/subscribe! http://www.chicagonow.com/downsize-maybe/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From tbraud at holyredeemer.com Thu Oct 29 13:32:35 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 29 Oct 2015 18:32:35 +0000 Subject: [Histonet] test cost Message-ID: <48E053DDF6CE074DB6A7414BA05403F8045E60@HRHEX02-HOS.holyredeemer.local> Do these steps in order: Take the total cost of your supplies + total hours salary average, for set period (usually use 6 months), and then divide by the number of blocks for a cost per block (ie. 0.80/block) Do the same for slides to arrive at a cost per slide. (ie. 1.25/stained slide) Calculate the average costs of a block and slide by taking the average (cost of block + cost of slide, then divide by 2 = average cost per block/slide Calculate the range of blocks per surg level during that same time period (ie. Lev V ranges from lowest 1blk, to highest 15blks) Calculate the range of test cost per surg level by (lowest block # X average cost per block/slide) = lowest cost, bottom of range Calculate the range of test cost per surg level by (highest block # X average cost per block/slide) = highest cost, top of range Show the math. Give them the range and average for each level. Add a paragraph explaining added costs of reference lab work, special stains, and other charges (CPT codes) that could be incurred in order to report out a case. Sometimes it is very difficult for administrators to understand how one CPT code may cost $5.80 or $25.00 depending on varying factors such as source of specimen or pathologist, and what is average for you, may not be average for another lab with different pathologists or cutting protocols. Keep it short and sweet! Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 From brannon at alliedsearchpartners.com Thu Oct 29 13:43:39 2015 From: brannon at alliedsearchpartners.com (Brannon Owens) Date: Thu, 29 Oct 2015 18:43:39 +0000 Subject: [Histonet] Mohs Assignment in Cincinnati, OH / 4 months, part-time - flexible pay Message-ID: Hello Histonet, We are currently seeking an experienced Mohs tech for an extended (December 2015 - March 2016), part time position in Cincinnati, OH. This is a 3 day per week temporary Mohs assignment. Flexible pay being offered. If interested please email an updated resume back to brannon at alliedsearchpartners.com along with your salary requirements for the job. Companies enjoy greater employee retention by offering quality benefits. As the legal W-2 employer of record for contractors, our back-office pays the contract employees on a weekly basis and also offers the following ACA compliant benefits. * ? Health Insurance, Dental Insurance, Vision Insurance, Life Insurance * ? Accidental death and dismemberment insurance * ? 401K savings plan Thank you, Check out our article in I4Biz June's Addition "Allied Search Partners connecting Labs to Employees" http://www.i4biz.com/sales-marketing/allied-search-partners-connects-labs-to-employees/ To view a complete list of Allied Search Partners current openings go to: http://www.jobs.net/jobs/alliedsearchpartners/en-us/all-jobs/United-States/ Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 Direct Line: 407.413.9421 F: 888.388.7572 From ASelf at tidelandshealth.org Thu Oct 29 13:43:49 2015 From: ASelf at tidelandshealth.org (Amy Self) Date: Thu, 29 Oct 2015 14:43:49 -0400 Subject: [Histonet] FW: CAP Checklist Question Message-ID: Histonetters, I am going through the CAP checklist and was wondering how the following question below is being handled by everyone that gets inspected by CAP. ANP.12360 Report Completeness Surgical pathology reports for specimens with malignant diagnosis from definitive cancer resections are outlined in the CAP Cancer Protocols are audited annually to ensure that all required elements are included. Thanks in advance for your help, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From LRaff at uropartners.com Fri Oct 30 09:21:51 2015 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 30 Oct 2015 14:21:51 +0000 Subject: [Histonet] A Halloween Blog for all of you Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B4AC0E4@COLOEXCH01.uropartners.local> You may want to share this one with your phlebotomists..and your pathologists! Happy Halloween. http://www.chicagonow.com/downsize-maybe/2015/10/sucking-a-little-less-blood-is-it-trick-or-treat/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From wyffels.jen at gmail.com Fri Oct 30 10:49:46 2015 From: wyffels.jen at gmail.com (Jennifer Wyffels) Date: Fri, 30 Oct 2015 11:49:46 -0400 Subject: [Histonet] sample BRIGHT yellow color change immediately upon fixation Message-ID: <2D47A3B9-27A3-4C46-99AB-D3854D92B9B5@gmail.com> I am fixing a sample that has a lot of glycoprotein in a gel-like matrix using a freshly prepared EM grade glut/para mixture in 0.1M Sorensons buffer. I have fixed many samples previously but have one species where the sample turns from mostly clear to bright yellow but still transparent within a minute of adding fixative. I?m searching for an explanation but was unable to find a similar description for other samples. Note the fixative is fresh and not yellow at the onset. Would appreciate any information. Thanks, Jen From jennjennhisto at gmail.com Fri Oct 30 11:06:18 2015 From: jennjennhisto at gmail.com (Jennifer Robinson) Date: Fri, 30 Oct 2015 09:06:18 -0700 Subject: [Histonet] Mildew on paraffin block Message-ID: I believe I may know the answer, but I would like peer support in my theory or an alternative answer. I'm looking for ideas regarding mildew causation on tissue in paraffin blocks. Details: outside blocks cut last week, placed wet (a no, no) back into a plastic bag, and growth found on tissue in blocks yesterday. Blocks cut before months ago with no problems, blocks show proper processing, and previous slides show proper fixation. Causation? And can blocks be salvaged with confidence in tissue integrity? Your feedback will be greatly appreciated. From rsrichmond at gmail.com Sat Oct 31 17:16:09 2015 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 31 Oct 2015 18:16:09 -0400 Subject: [Histonet] CAP Checklist Question Message-ID: Amy Self, Histology Lab Senior Tech, Tidelands Georgetown Memorial Hospital in Georgetown SC asks: >>I am going through the CAP checklist and was wondering how the following question below is being handled by everyone that gets inspected by CAP. ANP.12360 Report Completeness: Surgical pathology reports for specimens with malignant diagnosis from definitive cancer resections are outlined in the CAP Cancer Protocols are audited annually to ensure that all required elements are included.<< Your hospital should have some sort of cancer registry that's doing this, and your pathologist probably gets occasional requests to amplify reports. Ask your pathologists about this. Do you have an anatomic pathology application that generates CAP templates automatically? There are a number of these, such as mTuitive, which I've used successfully. Bob Richmond Samurai Pathologist Maryville TN