[Histonet] Duraedge (Black) help

[Rene J Buesa]

Extremely rare this problem because almost always thick/thin sections has nothing to do with the blades but with the microtome that is unable to hold the blade firmly.Are the blades in this microtome "thinner" than the others used in it previously?If they are, you could add a small piece of aluminum foil surrounding the thinner blade to make it thicker and perhaps enabling the blade holder in this microtome to hold them tighter. Just a thought.René 


    On Monday, November 30, 2015 11:54 AM, Hans B Snyder via Histonet <histonet at lists.utsouthwestern.edu> wrote:
 

 Hello All,

I recently switched from Duraedge (red) to Duraedge (black) blades.  This
has caused cutting problems on 1 microtome, our Leica RM 2235 microtome but
no others.  The sections are now thick and thin, compressed or lengthwise
ribbon splits.

I tried changing the blade holder angle, 6 forward and 6 back positions
with no luck.  After trying 12 different knife angles with no luck, I had
someone PM it and it's still not working. The original red blades still
work in the microtome and I don't understand how only 1 microtome does not
work with these blades.  Instead of buying a whole new case of red blades
just for this 1 microtome, I thought you might have some insight that could
help.

What should I do?

Thank you

Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
hans at histologistics.com <haqns at histologistics.com>

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