[Histonet] Aqueous Mounting Media

[Mehmet Fatih BOZKURT]

Hello, I’ve been used many different AMM's (from many companies). In
my experience
lab-made AMM is the best, there is *modified method *in ihcworld.com. I’m
using this method; slides are good even one year past. And no bubbles.

On Wed, Nov 4, 2015 at 4:08 PM, Keyser Gerald T via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Currently my lab is using Aqua-mount (Lerner Laboratories). I'm looking at
> replacing it with a different Aqueous mounting media. The only stain we use
> it for is Oil Red O.
>
> I've tried many different variations on its use and the results have been
> the same.
>
> 1)      The slide looks lovely the day that the coverslip is mounted and
> then dozens of bubbles form after. Sealing does not make a difference.
> Following company recommended procedure doesn't make a difference. Getting
> creative doesn't make a difference. Nothing seems to make a difference.
>
> 2)      We use a non-aqueous media surrounding the aqueous and
> post-coverslip the slide. This option is messy and cannot be
> re-coverslipped (for obvious reasons) if there is an air bubble. I'm sure
> that this wouldn't work at all with a more delicate stain.
>
> Ok. I give up. Can anyone make a recommendation for a different aqueous
> mounting media?
>
> Gerry
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Asst. Prof. Dr. Mehmet Fatih BOZKURT
Department of Pathology
Faculty of Veterinary Medicine
Afyon Kocatepe University
03100, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-16173/16237