From tkngflght at yahoo.com Sun Nov 1 00:38:52 2015 From: tkngflght at yahoo.com (Cheryl) Date: Sat, 31 Oct 2015 22:38:52 -0700 Subject: [Histonet] Hey guys- Histo Job in NC Message-ID: <4954A92F-0631-4B93-AEA4-0DF1C2336DC6@yahoo.com> Full time with benefits. More info available. Leave a message and I'll call you back - shoot me a resume as it makes the conversation easier!! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.883.7704 cell. Please excuse typos-sent from a phone. From garreyf at gmail.com Sun Nov 1 07:41:03 2015 From: garreyf at gmail.com (Garrey Faller) Date: Sun, 1 Nov 2015 08:41:03 -0500 Subject: [Histonet] Secondary specimen container labelling: New CAP checklist requirement: COM.06200 Message-ID: Hi everyone, I just noticed this new CAP requirement. Its in the All common checklist. I always thought slides and blocks must have two separate patient identifiers. Now, I guess slides and blocks are considered "secondary containers" and need only one. Am I reading this correctly? Our lab is 100% manual and we handwrite slides and blocks. I will continue to use two identifiers. Just thought Id bring this to your attention and see how you feel. Garrey *COM.06200* *Secondary Specimen Container Labeling* *Adequate specimen identification is provided on specimen containers throughout all phases of testing, including, but not limited to aliquots, dilution, tubes, slides, blocks, culture plates, reaction units, nucleic acids and other extracts, data extract files, images, and other secondary specimens created during the processing or testing of a specimen.* *NOTE: A single, unique identifier may be used to label materials derived from the primary specimen for use in subsequent phases of testing. The specimen identification system used must provide reliable identification of the secondary specimen and be linked to the full particulars of patient identification, collection date, specimen type, etc. The specimen identifier(s) must* *be indelible, legible, and able to withstand all stages of processing and conditions of storage. Identification may be text-based, numeric, bar-coded, etc. The form of this system is entirely at the discretion of each laboratory and must be defined in laboratory procedure.* *Slides prepared from specimens in the laboratory are considered secondary specimen containers. Slides prepared in the patient setting and brought to the laboratory (e.g. fine needle aspiration, bone marrow preparations) are considered primary specimen containers and must follow the labeling requirements for primary specimen containers.* *For histology specimens, each block of tissue must be identified by a unique identifier traceable to the primary specimen (e.g. accession number) assigned to the case and by any descriptive letter(s)/number(s) added by the prosector during the dissection. If additional blocks are prepared later, all lists and logs must reflect these additions. Identification number and letter(s)/number(s) must be affixed to all blocks in a manner that remains legible. Each slide must be identified by the unique identifier traceable to the primary specimen and descriptive letters unique to the block from which it is cut. Other appropriate identifiers should be included as applicable (e.g. levels of sectioning). Automated prelabeling systems are acceptable.* From LRaff at uropartners.com Mon Nov 2 08:03:26 2015 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 2 Nov 2015 14:03:26 +0000 Subject: [Histonet] Weekend post-reheated. Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B4BC378@COLOEXCH01.uropartners.local> Hope everyone had a good Halloween and welcome to November! Read/comment/subscribe, and please pass on to anyone you know who has had a prostate biopsy. http://www.chicagonow.com/downsize-maybe/2015/11/solar-panels-thumbs-up-or-thumbs-down/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From s.murdoch at dermpathnw.com Mon Nov 2 10:49:50 2015 From: s.murdoch at dermpathnw.com (Stacey Murdoch) Date: Mon, 2 Nov 2015 08:49:50 -0800 Subject: [Histonet] Job Opportunity Message-ID: <563794b3.416d440a.13f29.fffffcd8@mx.google.com> We are a small, busy dermatopathology lab in Bellevue, WA (Seattle Metro) looking for full time histologist. We provide a competitive salary and excellent benefits including medical and dental insurance, paid holidays, PTO, profit sharing, and 401K options. REQUIREMENTS . ASCP certification (HT/HTL) . 2 years laboratory experience required . IHC experience preferred . Ability to cut and embed small skin biopsy specimens efficiently . Good interpersonal skills . Self direction in a fast paced environment . Ability to work Tuesday-Saturday 4am - 12 pm with occasional overtime Interested parties may contact me directly for additional information at s.murdoch at dermpathnw.com Thanks, Stacey From kcai at prosci-inc.com Mon Nov 2 11:43:33 2015 From: kcai at prosci-inc.com (Karen Cai) Date: Mon, 2 Nov 2015 09:43:33 -0800 Subject: [Histonet] Help: IHC service Message-ID: <006f01d11596$00ee9b10$02cbd130$@com> HELP: Hi, Is there anybody can provide me the price list/structure of the custom IHC services? For example, to test 200 tissue slides and get 200 images, how much does it cost? Thank you very much in advance, Have a nice weekend, Best Regards, Karen kcai at prosci-inc.com From Colleen_Herring at bshsi.org Mon Nov 2 12:15:52 2015 From: Colleen_Herring at bshsi.org (Herring, Colleen) Date: Mon, 2 Nov 2015 18:15:52 +0000 Subject: [Histonet] H.Pylori Message-ID: <3AE5857C7EC73F4E983F39C97DF811C02C6B792B@EDC-EXMB4-01.ads.bshsi.com> Can anyone please help me with thiis problem. We have been doing H.Pyloris on the ultra for some time now and just recently we have been getting complaints from the Pathologists that there is a parcipitate on the tissue and slide. Has anyone else run into this problem and is there a solution. thank You in advance ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From craigak12 at gmail.com Mon Nov 2 12:57:01 2015 From: craigak12 at gmail.com (Jb) Date: Mon, 2 Nov 2015 11:57:01 -0700 Subject: [Histonet] Cassette Printer: Message-ID: <49C06453-15A5-4A73-A243-CC2C6AF0D6FA@gmail.com> What type of cassette printer is preferred by histotechs? I am looking for a quick, durable, cassette printer. We have a current volume of 150-200 + blocks a day. Any suggestions? Thank you, Craig Sent from my iPhone From cforster at umn.edu Mon Nov 2 14:24:29 2015 From: cforster at umn.edu (Colleen Forster) Date: Mon, 2 Nov 2015 14:24:29 -0600 Subject: [Histonet] Help: IHC service In-Reply-To: <006f01d11596$00ee9b10$02cbd130$@com> References: <006f01d11596$00ee9b10$02cbd130$@com> Message-ID: I'd be very interested in this as well...could you shaere Karen. Thanks. C On Mon, Nov 2, 2015 at 11:43 AM, Karen Cai via Histonet < histonet at lists.utsouthwestern.edu> wrote: > HELP: > > > > Hi, > > Is there anybody can provide me the price list/structure of the custom IHC > services? > > > > For example, to test 200 tissue slides and get 200 images, how much does it > cost? > > > > Thank you very much in advance, > > > > Have a nice weekend, > > > > Best Regards, > > Karen > > kcai at prosci-inc.com > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From twheelock at mclean.harvard.edu Mon Nov 2 15:05:32 2015 From: twheelock at mclean.harvard.edu (Wheelock, Timothy R.) Date: Mon, 2 Nov 2015 21:05:32 +0000 Subject: [Histonet] Solvent recycling Message-ID: <69718C0B0B3C414D9F8E7214AD400CC997DCA6EC@PHSX10MB11.partners.org> Hi Everyone: My hospital recently has been looking into recycling solvents. Our department alone does not have the volume to justify such an expense. However, there are many research labs on the campus that produce varying (probably small) quantities of solvent. They have asked me to look into the issue. When I was working in a large surgical histology lab many years ago, we never had any solvent recycling, so please forgive my basic questions. When I contemplate this issue, many issues arise: How big would the apparatus be (I am assuming they come in different sizes)? How much do they cost? Where would it have to be located? Do different solvents require different recycling machines? Who would be responsible for the machine? If it were a shared instrument, it may increase the likelihood of malfunctions. How much preventative maintenance does it require? I am assuming that training would be needed for all users. How comfortable would we and researchers be in using recycled solvents, as opposed to just buying new reagents each time? Anyway, I would appreciate any advice on this issue. Thanks you so much Tim Wheelock Harvard Brain Tissue Resource Center McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From brett_connolly at merck.com Tue Nov 3 07:32:58 2015 From: brett_connolly at merck.com (Connolly, Brett M) Date: Tue, 3 Nov 2015 08:32:58 -0500 Subject: [Histonet] CD8 IHC on FFPE mouse tissue -what your experience? Message-ID: Hi all, I have a project that will be looking at CD8 expression in some FFPE murine tissue. Past discussions here have mentioned the 14-0808 CD8a antibody from eBiosicence. For those of you have had the opportunity to use this antibody I would like to hear your experiences/ recommendations about the staining results. I'll need to do some quantitation of the staining so I am hoping this antibody is strong and specific. Thanks much, Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From histology81176 at att.net Tue Nov 3 09:03:53 2015 From: histology81176 at att.net (Histology Technician) Date: Tue, 3 Nov 2015 15:03:53 +0000 (UTC) Subject: [Histonet] Symphony users References: <1112149269.666168.1446563033498.JavaMail.yahoo@mail.yahoo.com> Message-ID: <1112149269.666168.1446563033498.JavaMail.yahoo@mail.yahoo.com> Symphony users, can you message me?? I want to talk about prices, what you like/dislike about it?off line.? Thanks! From LRaff at uropartners.com Tue Nov 3 09:14:31 2015 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 3 Nov 2015 15:14:31 +0000 Subject: [Histonet] A blogpost for parents, or the children of parents. Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B4C7156@COLOEXCH01.uropartners.local> Hello Netters--- Something to bring a smile to your "leisure" time. Click here for blog Read this one, because we have all been there! Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From aeck at dh.org Tue Nov 3 09:57:26 2015 From: aeck at dh.org (Eck, Allison) Date: Tue, 3 Nov 2015 15:57:26 +0000 Subject: [Histonet] (no subject) Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A971A5AB6@DH-MAIL01.dhorg.org> How many of you have a cytology dept where your cyto techs do all their own prep and reports? If you don't, do you have a cyto prep aide or does a histo tech help? Also, how many of you have histo and cyto as the same department with an AP manager? Does the AP manager oversee transcription as well Thank you in advance From LRaff at uropartners.com Tue Nov 3 10:02:25 2015 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 3 Nov 2015 16:02:25 +0000 Subject: [Histonet] FW: A blogpost for parents, or the children of parents.--problem with link?? Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B4C722D@COLOEXCH01.uropartners.local> If the previous link did not work in your browser, try the full address http://www.chicagonow.com/downsize-maybe/2015/11/if-you-are-a-parent-or-have-ever-had-one-the-forever-plan-is-for-you/ Read, comment, subscribe Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: Lester Raff MD via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, November 03, 2015 9:15 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] A blogpost for parents, or the children of parents. Hello Netters--- Something to bring a smile to your "leisure" time. Click here for blog Read this one, because we have all been there! Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster at umn.edu Tue Nov 3 10:30:16 2015 From: cforster at umn.edu (Colleen Forster) Date: Tue, 3 Nov 2015 10:30:16 -0600 Subject: [Histonet] CD8 IHC on FFPE mouse tissue -what your experience? In-Reply-To: References: Message-ID: I am interested to see what you hear Brett....I need to do these same markers and am not having good luck in FFPE....frozens , great...FFPE NOT! C On Tue, Nov 3, 2015 at 7:32 AM, Connolly, Brett M via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all, > I have a project that will be looking at CD8 expression in some FFPE > murine tissue. Past discussions here have mentioned the 14-0808 CD8a > antibody from eBiosicence. > > For those of you have had the opportunity to use this antibody I would > like to hear your experiences/ recommendations about the staining results. > > I'll need to do some quantitation of the staining so I am hoping this > antibody is strong and specific. > > Thanks much, > Brett > > Brett M. Connolly, Ph.D. > Prin. Scientist, > Translational Biomarkers - Imaging > Merck & Co., Inc. > PO Box 4, WP-44K > West Point, PA 19486 > brett_connolly at merck.com > T- 215-652-2501 > F- 215-993-6803 > > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, > New Jersey, USA 07033), and/or its affiliates Direct contact information > for affiliates is available at > http://www.merck.com/contact/contacts.html) that may be confidential, > proprietary copyrighted and/or legally privileged. It is intended solely > for the use of the individual or entity named on this message. If you are > not the intended recipient, and have received this message in error, > please notify us immediately by reply e-mail and then delete it from > your system. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From raestask at grics.net Tue Nov 3 10:31:50 2015 From: raestask at grics.net (raestask at grics.net) Date: Tue, 3 Nov 2015 11:31:50 -0500 (EST) Subject: [Histonet] Acid hematin Message-ID: <1756686661.39205007.1446568310450.JavaMail.root@grics.net> I saw a Histonet post that Peggy Wenk had made concerning the recipe for alcoholic formalin for processing.?She noted that you use the solution unbuffered.? How do you control acid hematin pigment in such a solution? We use a commercial alcoholic formalin ?concentrate? that is diluted with alcohol and water prior to use.? It is supposedly buffered.? We have great problems with acid hematin.? Do you have any suggestions?? For preprocessing fixation, we make our own buffered formalin from scratch or from a concentrate and get biopsy specimens from veterinarians fixed in who-knows-what. Thank you very much, DW ? Card ? From kiran_g at sbcglobal.net Tue Nov 3 13:20:55 2015 From: kiran_g at sbcglobal.net (Kiranjit Grewal) Date: Tue, 3 Nov 2015 19:20:55 +0000 (UTC) Subject: [Histonet] LFABP and PHH3 Abs References: <1473107417.1490045.1446578455633.JavaMail.yahoo@mail.yahoo.com> Message-ID: <1473107417.1490045.1446578455633.JavaMail.yahoo@mail.yahoo.com> Dear Experts, We are trying to optimize LFABP( Liver marker) and PHH3( Mitosis marker). Anyone willing to share their protocol or suggestions that can help expedite the process is much appericiated. We have both Leica & Ventana instruments. thank you in advance! Kiran From loree.lager at seattlechildrens.org Tue Nov 3 14:37:04 2015 From: loree.lager at seattlechildrens.org (Lager, Loree) Date: Tue, 3 Nov 2015 20:37:04 +0000 Subject: [Histonet] Pas Digestion Message-ID: <1B0FE3638CD0BE45A92BBA2E314B068250A21C1E@PPWEXD01d.childrens.sea.kids> Hello, This is my first time using the histonet, as our veteran user retired. We've been having an intermittent problem with incomplete glycogen digestion on PASD. Our digestion solution is 0.5% ?-Amylase, Type VI-B from porcine pancreas (Sigma), which we freeze in small aliquots and thaw for 45 minutes at room temperature before use. We digest for 20 minutes @ room temperature, dropping amylase on slides. In troubleshooting the issue, we discovered amylase was several years old, so new amylase ordered. The new lot (while the same product number) produced even worse results. After a closer look we saw that the new lot contained ? of the units/mg solid, 26 to 13, not sure why? Anyone willing to share their procedure or suggestions to help us resolve this issue? Thank you, Loree Lager Seattle Children's Hospital Histology CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From patpxs at gmail.com Tue Nov 3 15:02:36 2015 From: patpxs at gmail.com (P Sicurello) Date: Tue, 3 Nov 2015 13:02:36 -0800 Subject: [Histonet] Paraffin sectioning for DNA analysis Message-ID: Hello Listers, Which techniques are you all using to avoid cross contamination when cutting multiple sections from multiple blocks for DNA analysis? Way back I used to use DEP-C treated water, wore gloves, changed the blade between blocks and wiped down the surface of the knife holder plate with the DEP-C water and ETOH. What is the currently accepted procedure? Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From JMacDonald at mtsac.edu Tue Nov 3 16:54:19 2015 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Tue, 3 Nov 2015 14:54:19 -0800 Subject: [Histonet] Pas Digestion In-Reply-To: <1B0FE3638CD0BE45A92BBA2E314B068250A21C1E@PPWEXD01d.childrens.sea.kids> References: <1B0FE3638CD0BE45A92BBA2E314B068250A21C1E@PPWEXD01d.childrens.sea.kids> Message-ID: The optimal temperature for most enzymes in 37 degrees Celsius. From: "Lager, Loree via Histonet" To: "'histonet at lists.utsouthwestern.edu'" Date: 11/03/2015 12:38 PM Subject: [Histonet] Pas Digestion Hello, This is my first time using the histonet, as our veteran user retired. We've been having an intermittent problem with incomplete glycogen digestion on PASD. Our digestion solution is 0.5% ?-Amylase, Type VI-B from porcine pancreas (Sigma), which we freeze in small aliquots and thaw for 45 minutes at room temperature before use. We digest for 20 minutes @ room temperature, dropping amylase on slides. In troubleshooting the issue, we discovered amylase was several years old, so new amylase ordered. The new lot (while the same product number) produced even worse results. After a closer look we saw that the new lot contained ? of the units/mg solid, 26 to 13, not sure why? Anyone willing to share their procedure or suggestions to help us resolve this issue? Thank you, Loree Lager Seattle Children's Hospital Histology CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr at comcast.net Tue Nov 3 21:31:00 2015 From: koellingr at comcast.net (koellingr at comcast.net) Date: Wed, 4 Nov 2015 03:31:00 +0000 (UTC) Subject: [Histonet] Pas Digestion In-Reply-To: <1B0FE3638CD0BE45A92BBA2E314B068250A21C1E@PPWEXD01d.childrens.sea.kids> References: <1B0FE3638CD0BE45A92BBA2E314B068250A21C1E@PPWEXD01d.childrens.sea.kids> Message-ID: <1613471281.1729231.1446607860923.JavaMail.zimbra@comcast.net> As Jennifer mentioned, enzymes have optimal temperatures at which to work.? But also optimal pH.? Is your 0.5% amylase in water or a correct buffer? Ray, Seattle, WA ----- Original Message ----- From: "Loree via Histonet Lager" To: "histonet at lists.utsouthwestern.edu" Sent: Tuesday, November 3, 2015 12:37:04 PM Subject: [Histonet] Pas Digestion Hello, This is my first time using the histonet, as our veteran user retired. We've been having an intermittent problem with incomplete glycogen digestion on PASD. ?Our digestion solution is 0.5% ??-Amylase, Type VI-B from porcine pancreas (Sigma), which we freeze in small aliquots and thaw for 45 minutes at room temperature before use. We digest for 20 minutes @ room temperature, dropping amylase on slides. In troubleshooting the issue, we discovered amylase was several years old, so new amylase ordered. ? The new lot (while the same product number) produced even worse results. ?After a closer look we saw that the new lot contained ? of the units/mg solid, 26 to 13, not sure why? Anyone willing to share their procedure or suggestions to help us resolve this issue? Thank you, Loree Lager Seattle Children's Hospital Histology CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GKeyser at uwhealth.org Wed Nov 4 08:08:41 2015 From: GKeyser at uwhealth.org (Keyser Gerald T) Date: Wed, 4 Nov 2015 14:08:41 +0000 Subject: [Histonet] Aqueous Mounting Media Message-ID: <5226C88C65EBFF4BAD552D68DC6E8FFE076052@UWHC-MBX12.uwhis.hosp.wisc.edu> Currently my lab is using Aqua-mount (Lerner Laboratories). I'm looking at replacing it with a different Aqueous mounting media. The only stain we use it for is Oil Red O. I've tried many different variations on its use and the results have been the same. 1) The slide looks lovely the day that the coverslip is mounted and then dozens of bubbles form after. Sealing does not make a difference. Following company recommended procedure doesn't make a difference. Getting creative doesn't make a difference. Nothing seems to make a difference. 2) We use a non-aqueous media surrounding the aqueous and post-coverslip the slide. This option is messy and cannot be re-coverslipped (for obvious reasons) if there is an air bubble. I'm sure that this wouldn't work at all with a more delicate stain. Ok. I give up. Can anyone make a recommendation for a different aqueous mounting media? Gerry From mills at 3scan.com Wed Nov 4 09:21:21 2015 From: mills at 3scan.com (Caroline Miller) Date: Wed, 4 Nov 2015 07:21:21 -0800 Subject: [Histonet] Pas Digestion In-Reply-To: References: <1B0FE3638CD0BE45A92BBA2E314B068250A21C1E@PPWEXD01d.childrens.sea.kids> Message-ID: Yes, 37oC is optimal, however an enzyme is often active at 25oC but to a lower level of efficiency....you just leave it longer. I don't know if you ever did this in the US, but when I first learned the dPAS technique we used to spit on the slide :) Much nicer is to buy some diastase such as this: http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=AHDIA50 and I have attached what we used to do in London (the spitting was only a good trick to show the trainees) Maybe it is the freezing? Enzymes don't really like that unless there is glycerol and /or a high concentration of enzyme. I would just make it up from powder fresh every time, it is not that hard or time consuming yours, mills On Tue, Nov 3, 2015 at 2:54 PM, Jennifer MacDonald via Histonet < histonet at lists.utsouthwestern.edu> wrote: > The optimal temperature for most enzymes in 37 degrees Celsius. > > > > > From: "Lager, Loree via Histonet" > To: "'histonet at lists.utsouthwestern.edu'" > > Date: 11/03/2015 12:38 PM > Subject: [Histonet] Pas Digestion > > > > Hello, > > This is my first time using the histonet, as our veteran user retired. > > We've been having an intermittent problem with incomplete glycogen > digestion on PASD. Our digestion solution is 0.5% ?-Amylase, Type VI-B > from porcine pancreas (Sigma), which we freeze in small aliquots and thaw > for 45 minutes at room temperature before use. We digest for 20 minutes @ > room temperature, dropping amylase on slides. > > In troubleshooting the issue, we discovered amylase was several years old, > so new amylase ordered. The new lot (while the same product number) > produced even worse results. After a closer look we saw that the new lot > contained ? of the units/mg solid, 26 to 13, not sure why? > > Anyone willing to share their procedure or suggestions to help us resolve > this issue? > > Thank you, > Loree Lager > Seattle Children's Hospital > Histology > > > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information protected by law. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender by reply e-mail and destroy all > copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From samjr2 at u.washington.edu Wed Nov 4 12:05:56 2015 From: samjr2 at u.washington.edu (Sammie) Date: Wed, 4 Nov 2015 10:05:56 -0800 (PST) Subject: [Histonet] DR1 and DAT Message-ID: Hello, Trying again...I am trying to optimize DR1 - Dopamine receptor and DAT - Dopamine transporter. Anyone willing to share their protocol or suggestions that can help expedite the process is much appreciated. I use a Bond Max - Leica Thanks Sammie From jamie.erickson at abbvie.com Wed Nov 4 13:00:24 2015 From: jamie.erickson at abbvie.com (Erickson, Jamie E) Date: Wed, 4 Nov 2015 19:00:24 +0000 Subject: [Histonet] CD8 IHC on FFPE mouse tissue -what your experience? Message-ID: <69cb3ecc7e4b4d5fb053f023a52d930a@USAASECSM025.R0018.COLLABORATION.ECS.HP.COM> Hi Brett, We love this CD8 and the CD4 on FFPE samples we have tested them on mouse spleen, thymus, and small intestine. The CD8a stains nicely at 0.075ug/ml. That being said we use a Leica BOND RX to stain our samples which has a Rabbit IgG polymer detection which amplifies our signal. We do a heat step called H2 20 which is ( EDTA) for 20 minutes at ~ 95C. Primary antibody concentration 0.075ug/ml 30 min incubation Secondary antibody Rabbit anti-Rat Vector labs cat # AI-4001 concentration 10ug/ml incubation 20 minutes. I uploaded an image at: http://histosearch.com/imageupload/ We have not done any quantitation with this yet but I'm sure it would work nicely. Jamie Erickson Scientist Foundational Immunology Abbvie Laboratories Worcester, MA 01605 Jamie.erickson at abbvie.com T-508-688-3134 From FMonson at wcupa.edu Wed Nov 4 13:15:26 2015 From: FMonson at wcupa.edu (Monson, Frederick) Date: Wed, 4 Nov 2015 19:15:26 +0000 Subject: [Histonet] Certification - Part I Message-ID: <515d8e05cd3644d3b0185150f4b0f405@WCUXCHP08.PASSHE.LCL> Halloween story of a 'tech' who lacked a certificate. T'was a Sunday, and he, a student at the age of 24, was "ON" for the weekend covering blood bank, chemistry, and hematology. A call came in for a unit of blood for a Friday surgical - just to bring the patient up to speed. He, for reasons never given, decided to practice 'crossing' by doing up 10 units - all of which passed. One he sent to the patient. Two hours later he received a call for another unit, because, as he was informed, his hematocrit was unchanged. What did he do? Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pennsylvania Schmucker Science South - Room SSS-024 MailDrop: Geology-Astronomy 750 South Church Street West Chester, PA, 19383 610-738-0437 fmonson at wcupa.edu From FMonson at wcupa.edu Wed Nov 4 13:22:52 2015 From: FMonson at wcupa.edu (Monson, Frederick) Date: Wed, 4 Nov 2015 19:22:52 +0000 Subject: [Histonet] Certification - II Message-ID: <17d1a31d08a34c05bc9af83d507ae8de@WCUXCHP08.PASSHE.LCL> He refused to send a unit without an order for a new cross match, which he got with some argument. Only 4 of the 9 previously good units passed as he re-crossed all ten, and the one he sent did not pass the second crossing. He called the pathologist on duty, who told him to give up going to medical school if he screwed up. After the pathologist repeated the two experiments, he said a prayer and offered a compliment. When asked how he had known, his response was, "I remembered the immunology I learned when I took microbiology 5 years earlier. He could not get that job now, I suspect, though the conversation of certification remind me of what I learned about the trades and 'certification' in ancient Egypt. Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pennsylvania Schmucker Science South - Room SSS-024 MailDrop: Geology-Astronomy 750 South Church Street West Chester, PA, 19383 610-738-0437 fmonson at wcupa.edu From carol.wilson at ricerca.com Wed Nov 4 13:24:21 2015 From: carol.wilson at ricerca.com (Wilson, Carol) Date: Wed, 4 Nov 2015 14:24:21 -0500 Subject: [Histonet] Bone Density in Rodent Femurs/Archimedes Principle Message-ID: <19848F1C0886A5409C729CC2136EDAFF631714C90C@IAD2MBX09.mex02.mlsrvr.com> Hi All, I am not having much luck finding exact information to perform bone density on rat femurs/tibias using Archimedes principle. Can anyone provide an exact procedure, or point me in the right direction? Including exact type of equipment used, etc. I have found many references with outlined instructions, but not exact enough for what I need. Thanks, Carol Carol Wilson, HT(ASCP) Associate Scientist III Team Leader/Histopathology Ricerca Biosciences, LLC From Karoleigh.Armstrong at ARMC.net Wed Nov 4 14:47:22 2015 From: Karoleigh.Armstrong at ARMC.net (Armstrong, Karoleigh T) Date: Wed, 4 Nov 2015 20:47:22 +0000 Subject: [Histonet] Job opening Message-ID: There is a job opening at Abilene Regional Medical Center, in Abilene TX, for a Histotechnician/Histologist. Abilene is located in Central Texas, a Medical hub for the region and has 3 local university, 2 Jr. colleges, a lively arts and culture. Duties include, but not limited to, embedding, cutting, routine H&E staining, special staining, cytology prep and staining. Some clerical duties and QA/QC. Please contact, www.abileneregional.com Thanks, KK Armstrong H.T. (ASCP) -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From fbozkurt at gmail.com Thu Nov 5 05:15:10 2015 From: fbozkurt at gmail.com (Mehmet Fatih BOZKURT) Date: Thu, 5 Nov 2015 13:15:10 +0200 Subject: [Histonet] Aqueous Mounting Media In-Reply-To: <5226C88C65EBFF4BAD552D68DC6E8FFE076052@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <5226C88C65EBFF4BAD552D68DC6E8FFE076052@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: Hello, I?ve been used many different AMM's (from many companies). In my experience lab-made AMM is the best, there is *modified method *in ihcworld.com. I?m using this method; slides are good even one year past. And no bubbles. On Wed, Nov 4, 2015 at 4:08 PM, Keyser Gerald T via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Currently my lab is using Aqua-mount (Lerner Laboratories). I'm looking at > replacing it with a different Aqueous mounting media. The only stain we use > it for is Oil Red O. > > I've tried many different variations on its use and the results have been > the same. > > 1) The slide looks lovely the day that the coverslip is mounted and > then dozens of bubbles form after. Sealing does not make a difference. > Following company recommended procedure doesn't make a difference. Getting > creative doesn't make a difference. Nothing seems to make a difference. > > 2) We use a non-aqueous media surrounding the aqueous and > post-coverslip the slide. This option is messy and cannot be > re-coverslipped (for obvious reasons) if there is an air bubble. I'm sure > that this wouldn't work at all with a more delicate stain. > > Ok. I give up. Can anyone make a recommendation for a different aqueous > mounting media? > > Gerry > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Asst. Prof. Dr. Mehmet Fatih BOZKURT Department of Pathology Faculty of Veterinary Medicine Afyon Kocatepe University 03100, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 From LRaff at uropartners.com Thu Nov 5 07:38:16 2015 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 5 Nov 2015 13:38:16 +0000 Subject: [Histonet] Todays suburban news! Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B4CBFC8@COLOEXCH01.uropartners.local> Morning, netters! Read on for nothing but the truth! http://www.chicagonow.com/downsize-maybe/2015/11/this-blog-is-based-on-true-events-really/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From GKeyser at uwhealth.org Thu Nov 5 07:49:23 2015 From: GKeyser at uwhealth.org (Keyser Gerald T) Date: Thu, 5 Nov 2015 13:49:23 +0000 Subject: [Histonet] Aqueous Mounting Media In-Reply-To: References: <5226C88C65EBFF4BAD552D68DC6E8FFE076052@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: <5226C88C65EBFF4BAD552D68DC6E8FFE076113@UWHC-MBX12.uwhis.hosp.wisc.edu> The slides are an important part of the medical record. Sure? I could assure that slide that are covered with media only are well treated while in my custody. But, those slides might see all sorts of abuse once they are outside of my care. They have to survive whatever pathologists, surgeons, residents, or fellows can throw at them. I feel better having a sturdy piece of glass over those sections. Gerry From: Caroline Miller [mailto:mills at 3scan.com] Sent: Wednesday, November 04, 2015 7:05 PM To: Keyser Gerald T Subject: Re: [Histonet] Aqueous Mounting Media Is there a reason you have to coverslip? I am presuming for storage. But I have found that the aqueous media alone is fine for imaging / viewing. If you are careful storing them they should be fine too. I use this: http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=MMC0619 and I take a piece of parafilm, make sure the slide is wet, but not dripping (I take it out and give it a quick flick) put a drop of the aquaslip on the slide and use capillary action to pull it across the section, you have to be careful not to scratch the sample (make sure there are no nicks in the parafilm). It gives a nice flat surface, but it sounds like you are also getting some off-gassing from the product. Oh Science! Good luck! mills On Wed, Nov 4, 2015 at 6:08 AM, Keyser Gerald T via Histonet > wrote: Currently my lab is using Aqua-mount (Lerner Laboratories). I'm looking at replacing it with a different Aqueous mounting media. The only stain we use it for is Oil Red O. I've tried many different variations on its use and the results have been the same. 1) The slide looks lovely the day that the coverslip is mounted and then dozens of bubbles form after. Sealing does not make a difference. Following company recommended procedure doesn't make a difference. Getting creative doesn't make a difference. Nothing seems to make a difference. 2) We use a non-aqueous media surrounding the aqueous and post-coverslip the slide. This option is messy and cannot be re-coverslipped (for obvious reasons) if there is an air bubble. I'm sure that this wouldn't work at all with a more delicate stain. Ok. I give up. Can anyone make a recommendation for a different aqueous mounting media? Gerry _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From histology81176 at att.net Thu Nov 5 08:27:56 2015 From: histology81176 at att.net (Histology Technician) Date: Thu, 5 Nov 2015 14:27:56 +0000 (UTC) Subject: [Histonet] Vantage users References: <1895645826.214656.1446733676134.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1895645826.214656.1446733676134.JavaMail.yahoo@mail.yahoo.com> Vantage users, what do you find most useful with using vantage?? Do you use it for just labeling purposes or do you find the reports helpful and what do you have on your home page that you find useful? Thanks! From blayjorge at gmail.com Thu Nov 5 08:38:49 2015 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Thu, 5 Nov 2015 09:38:49 -0500 Subject: [Histonet] new issue alert, free Message-ID: NEW ISSUE ALERT: Life The Excitement of Biology 3(3) http://blaypublishers.com/2015/10/31/leb-33-2015/ Apologies for potential duplicate emails. Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From elaineahoffman55 at yahoo.com Thu Nov 5 10:05:38 2015 From: elaineahoffman55 at yahoo.com (Elaine allison Hoffman) Date: Thu, 5 Nov 2015 16:05:38 +0000 (UTC) Subject: [Histonet] IHC hand staining References: <1174134951.280885.1446739538242.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1174134951.280885.1446739538242.JavaMail.yahoo@mail.yahoo.com> Greetings Histonetters, I need some information on "hand" immuno-staining.? We are a small GI lab doing Giemsa stains on H. Pylori right now, but the Lab Director wants me to look into IHC hand staining for H.Pylori.? Anybody out there doing hand staining and willing to give me a protocol to try? Or maybe point me in the right direction to who could help me with that.? Any reliable IHC staining kits recommended?? Thanks in advance for the help.... Elaine Hoffman The Gastroenterology Clinic & Endoscopy Center, IncGI Pathology Laboratory1622 E Market StreetWarren, Ohio?? 44483 From Albert.Santiago at uphs.upenn.edu Thu Nov 5 12:44:15 2015 From: Albert.Santiago at uphs.upenn.edu (Santiago, Albert) Date: Thu, 5 Nov 2015 18:44:15 +0000 Subject: [Histonet] Indirect protocol Message-ID: Hello my fellow histonetters, We are having difficulties with our Indirect immunofluorescent protocol, does anyone have a protocol they can share with me? Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market ST. Ste 201 Philadelphia, PA 19104 215-662-6539 - Lab 215-662-6759-office albert.santiago at uphs.upenn.edu From tbraud at holyredeemer.com Thu Nov 5 12:51:08 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 5 Nov 2015 18:51:08 +0000 Subject: [Histonet] Aquous Mounting Media Message-ID: <48E053DDF6CE074DB6A7414BA05403F8047A19@HRHEX02-HOS.holyredeemer.local> Have you tried Sigma's ImmunoHistoMount? It was what replaced Crystal Mount which used to be my favorite. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 ****** From supervisor at galahistolab.com Thu Nov 5 14:36:26 2015 From: supervisor at galahistolab.com (supervisor at galahistolab.com) Date: Thu, 05 Nov 2015 13:36:26 -0700 Subject: [Histonet] Leica Bond H. Pylori Message-ID: <20151105133626.19af9b47bafcece1f9503794462dcaf9.684526b146.wbe@email22.secureserver.net> From JMacDonald at mtsac.edu Thu Nov 5 17:20:14 2015 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Thu, 5 Nov 2015 15:20:14 -0800 Subject: [Histonet] IHC hand staining In-Reply-To: <1174134951.280885.1446739538242.JavaMail.yahoo@mail.yahoo.com> References: <1174134951.280885.1446739538242.JavaMail.yahoo.ref@mail.yahoo.com> <1174134951.280885.1446739538242.JavaMail.yahoo@mail.yahoo.com> Message-ID: In one of our classes the students do IHC staining by hand. They perform multiple procedures over the semester. We currently use Biocare reagents. The polymer based detection systems are simple to use. The data sheets that accompany the antibodies and detection reagents are really helpful with protocols. From: Elaine allison Hoffman via Histonet To: Histonet List Date: 11/05/2015 08:08 AM Subject: [Histonet] IHC hand staining Greetings Histonetters, I need some information on "hand" immuno-staining. We are a small GI lab doing Giemsa stains on H. Pylori right now, but the Lab Director wants me to look into IHC hand staining for H.Pylori. Anybody out there doing hand staining and willing to give me a protocol to try? Or maybe point me in the right direction to who could help me with that. Any reliable IHC staining kits recommended? Thanks in advance for the help.... Elaine Hoffman The Gastroenterology Clinic & Endoscopy Center, IncGI Pathology Laboratory1622 E Market StreetWarren, Ohio 44483 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From latecor at adinet.com.uy Thu Nov 5 17:38:40 2015 From: latecor at adinet.com.uy (Carlos Defeo) Date: Thu, 05 Nov 2015 23:38:40 +0000 Subject: [Histonet] IHC hand staining In-Reply-To: <1174134951.280885.1446739538242.JavaMail.yahoo@mail.yahoo.com> Message-ID: IHC staining for H.Pylori could be done using monoclonals from CellMarque or Biocare, with a simple protocol reccommended by the vendor, My regards, Carlos Histotochnologist ------ Mensaje original ------ De: "Elaine allison Hoffman via Histonet" Para: "Histonet List" Enviado: 05/11/2015 13:05:38 Asunto: [Histonet] IHC hand staining >Greetings Histonetters, >I need some information on "hand" immuno-staining. We are a small GI >lab doing Giemsa stains on H. Pylori right now, but the Lab Director >wants me to look into IHC hand staining for H.Pylori. Anybody out >there doing hand staining and willing to give me a protocol to try? Or >maybe point me in the right direction to who could help me with that. >Any reliable IHC staining kits recommended? Thanks in advance for the >help.... >Elaine Hoffman >The Gastroenterology Clinic & Endoscopy Center, IncGI Pathology >Laboratory1622 E Market StreetWarren, Ohio 44483 > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lldewe at gmail.com Thu Nov 5 17:43:07 2015 From: lldewe at gmail.com (Loralei Dewe) Date: Thu, 5 Nov 2015 15:43:07 -0800 Subject: [Histonet] IHC hand staining In-Reply-To: References: <1174134951.280885.1446739538242.JavaMail.yahoo@mail.yahoo.com> Message-ID: I learned how to do all my IHC by hand from processing and embedding the tissue to doing the cutting (manual microtome) and subsequent staining. It has really helped me in evaluating assays and in troubleshooting customers problems! Loralei Dewe Chrysalis Innovations On Nov 5, 2015 3:39 PM, "Carlos Defeo via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > IHC staining for H.Pylori could be done using monoclonals from CellMarque > or Biocare, with a simple protocol reccommended by the vendor, > My regards, > Carlos > Histotochnologist > > ------ Mensaje original ------ > De: "Elaine allison Hoffman via Histonet" < > histonet at lists.utsouthwestern.edu> > Para: "Histonet List" > Enviado: 05/11/2015 13:05:38 > Asunto: [Histonet] IHC hand staining > > Greetings Histonetters, >> I need some information on "hand" immuno-staining. We are a small GI lab >> doing Giemsa stains on H. Pylori right now, but the Lab Director wants me >> to look into IHC hand staining for H.Pylori. Anybody out there doing hand >> staining and willing to give me a protocol to try? Or maybe point me in the >> right direction to who could help me with that. Any reliable IHC staining >> kits recommended? Thanks in advance for the help.... >> Elaine Hoffman >> The Gastroenterology Clinic & Endoscopy Center, IncGI Pathology >> Laboratory1622 E Market StreetWarren, Ohio 44483 >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ahughes8 at ITS.JNJ.com Fri Nov 6 12:50:33 2015 From: ahughes8 at ITS.JNJ.com (Hughes, Anna [JRDUS]) Date: Fri, 6 Nov 2015 18:50:33 +0000 Subject: [Histonet] IHC hand staining In-Reply-To: References: <1174134951.280885.1446739538242.JavaMail.yahoo.ref@mail.yahoo.com> <1174134951.280885.1446739538242.JavaMail.yahoo@mail.yahoo.com> Message-ID: <1062C7BE2E82104EBB0231BDCBE87E2F02C04F63@ITSUSRAGMDGE05.jnj.com> Hi! I have done a lot of manual staining in the past....a simple protocol is below: Any of the Dako kits are wonderful...as are the Biocare 1. Depar slides as usual 2.Place slides in DPBS (no mag or calcium) 2x 5 minutes 3. Antigen retrieval if necessary - will have to look at the insert for your primary antibody. 4. Block Peroxidases for 10 minutes with 3% H2O2 5. Wash using DPBS 2x 5minutes 6. Protein block - 30 minutes using 5% normal serum of the host of your secondary antibody 7.Wash using DPBS 2x 5 minutes 8. Place slides in your primary antibody (I usually use 200-300ul/slide depending on tissue size and cover with a small piece of parafilm). Incubate in a humidity box at RT on the bench for 1 hour 9. Wash using DPBS 2x5 minutes 10. Place slides in a biotinylated secondary antibody (incubate same as primary above)- for 20 minutes 11. Wash using DPBS 2x5 mintues 12. Use a DAB detection kit such as the DAKO Envision kit - follow their directions in the kit. (These come as mouse,rabbit, rat...... so if you buy the kit as a whole, it comes with the biotinylated secondary (goat host) - already diluted and ready to use) 13. Wash 2x dH2O 14. Counterstain with Hematoxylin for 5 minutes 15. Wash with dH2O 2x 5 minutes 16. Blue slides -this will depend on your hematoxylin 17. Wash with dH2O 2x5minutes 18. Dehydrate through alcohols to xylene and coverslip. Hope this is helpful! Anna Hughes -----Original Message----- From: Jennifer MacDonald [mailto:JMacDonald at mtsac.edu] Sent: Thursday, November 05, 2015 6:20 PM To: Elaine allison Hoffman Cc: Histonet List Subject: Re: [Histonet] IHC hand staining In one of our classes the students do IHC staining by hand. They perform multiple procedures over the semester. We currently use Biocare reagents. The polymer based detection systems are simple to use. The data sheets that accompany the antibodies and detection reagents are really helpful with protocols. From: Elaine allison Hoffman via Histonet To: Histonet List Date: 11/05/2015 08:08 AM Subject: [Histonet] IHC hand staining Greetings Histonetters, I need some information on "hand" immuno-staining. We are a small GI lab doing Giemsa stains on H. Pylori right now, but the Lab Director wants me to look into IHC hand staining for H.Pylori. Anybody out there doing hand staining and willing to give me a protocol to try? Or maybe point me in the right direction to who could help me with that. Any reliable IHC staining kits recommended? Thanks in advance for the help.... Elaine Hoffman The Gastroenterology Clinic & Endoscopy Center, IncGI Pathology Laboratory1622 E Market StreetWarren, Ohio 44483 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sccrshlly at yahoo.com Fri Nov 6 17:15:52 2015 From: sccrshlly at yahoo.com (Shelly Coker) Date: Fri, 6 Nov 2015 23:15:52 +0000 (UTC) Subject: [Histonet] New IHC Lab set up References: <1735370736.1090464.1446851752297.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1735370736.1090464.1446851752297.JavaMail.yahoo@mail.yahoo.com> Hello everyone! I have been recruited to set up a new IHC lab for a small GI practice.? We are talking roughly 3,300 slides per year.? I am currently looking at the Dako Link 48 and the Leica Bond Max.? Price prevents the use of? Ventana system as we need to keep the total cost per slide below $15.? I like the open system of the Dako, but the Bond Max seems to be more user friendly.? Any thoughts from me fellow techs out there? Happy Friday!Shelly From LRaff at uropartners.com Sat Nov 7 07:58:46 2015 From: LRaff at uropartners.com (Lester Raff MD) Date: Sat, 7 Nov 2015 13:58:46 +0000 Subject: [Histonet] Blogoshpere Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B4D7BC2@COLOEXCH01.uropartners.local> Hello netters, Who is working on Saturday. If you aren't this should arrive for you whenever your work week starts. http://www.chicagonow.com/downsize-maybe/2015/11/five-things-we-get-from-our-parents/ Les Raff From elaineahoffman55 at yahoo.com Sat Nov 7 13:16:58 2015 From: elaineahoffman55 at yahoo.com (Elaine allison Hoffman) Date: Sat, 7 Nov 2015 19:16:58 +0000 (UTC) Subject: [Histonet] Competency Assessments References: <363990482.1097141.1446923818140.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <363990482.1097141.1446923818140.JavaMail.yahoo@mail.yahoo.com> Greeting everyone in Histo-land, I'm still working on a competency assessment procedure but I have a question that needs clarification.? According to CAP, "A laboratory must evaluate and document the competency of all testing personnel for each test system.? A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results.? A test system may be manual, automated, multi-channel or single use and can include reagents, components, equipment or instruments required to produce results".? My question is, what histology tasks or steps are considered pre-analytic, analytic, and post-analytic?? In all the resources I've found, there seems to be a difference of opinion as to what is considered pre-analytic, analytic, and post-analytic specific to histology tasks which is not really considered "resulting" in histology. Also, there should be a different TEST SYSTEM for what pathologists do (resulting slides) and what PA's do (gross description of tissue), so what tasks are considered pre-analytic, analytic, and post-analytic specific for their jobs? A person could go crazy with all this and to ask an inspector, they aren't really sure either.? And different inspectors will give you different answers.Any takers out there? Elaine Hoffman ? ? From pwnoyce at gmail.com Sat Nov 7 17:13:29 2015 From: pwnoyce at gmail.com (Peter Noyce) Date: Sun, 8 Nov 2015 10:13:29 +1100 Subject: [Histonet] plant tissue stain Message-ID: <000001d119b1$f3cfe950$db6fbbf0$@gmail.com> This may seem a bit odd for this forum but what is the best stain for plant tissue histology slides-to show nuclei, cytoplasm, cell wall. PW Noyce. From jamie at prometheushealthcare.com Sat Nov 7 17:36:25 2015 From: jamie at prometheushealthcare.com (Jamie Buxton) Date: Sat, 7 Nov 2015 18:36:25 -0500 Subject: [Histonet] Spencer from Promeheus Healthcare - Recruiter Message-ID: <004a01d119b5$1f3fe0b0$5dbfa210$@prometheushealthcare.com> Hi there, My name is Spencer Williams, Recruiter with Prometheus healthcare. We specialize in the recruitment and placement of lab professionals. We are currently recruiting for a well known laboratory in both the Chicago and Northern California area. We are assisting them in finding top Histotechnicians as well as Medical Technologist candidates for their laboratory. The position is a full time, permanent opportunity. If you may be interested in this opportunity, I'd love to discuss it further with you. Please feel free to reach out to me at any of my contacts below. Thank you in advance! Spencer Williams Recruiter Prometheus Healthcare Direct Line (407) 961-0186 Office (866) 857-1434 Fax (301) 368-2478 Spencer at prometheushealthcare.com www.prometheushealthcare.com From rjbuesa at yahoo.com Sun Nov 8 09:54:07 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Sun, 8 Nov 2015 15:54:07 +0000 (UTC) Subject: [Histonet] plant tissue stain In-Reply-To: <000001d119b1$f3cfe950$db6fbbf0$@gmail.com> References: <000001d119b1$f3cfe950$db6fbbf0$@gmail.com> Message-ID: <25590792.1240647.1446998047113.JavaMail.yahoo@mail.yahoo.com> I always used a green (Fast green) for cell walls and cytoplasm + a red (fuchsine) for nuclei.If you get Peter Gray's?book you will find numerous plant procedures. In Bolles-Lee (Microtomist's Vade-Mecum) there are also many methods for plant tissues.Ren? On Saturday, November 7, 2015 6:13 PM, Peter Noyce via Histonet wrote: This may seem a bit odd for this forum but what is the best stain for plant tissue histology slides-to show nuclei, cytoplasm, cell wall. PW Noyce. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Sun Nov 8 10:03:54 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Sun, 8 Nov 2015 16:03:54 +0000 (UTC) Subject: [Histonet] Competency Assessments In-Reply-To: <363990482.1097141.1446923818140.JavaMail.yahoo@mail.yahoo.com> References: <363990482.1097141.1446923818140.JavaMail.yahoo@mail.yahoo.com> Message-ID: <2135432179.1286090.1446998634673.JavaMail.yahoo@mail.yahoo.com> Elaine:As you wrote there are differences of opinion, so here is mine:Start with "analysis" which is the process of determining the qualities of something. As I see it, in histology the pathologist is the one who analyzes = determines the qualities of the tissue sections and gets to a diagnosis.Consequently, pre-analytical are all those steps leading to the preparation of the slide, namely, grossing, processing, sectioning and staining.The PT analyzes the finished tissue section and asks for special procedures; and those are post analytical but they can be also considered as part of a final diagnoses and, as such, are also "pre-analytical". If you take this last definition, then the post analytical will be limited to everything is done in the lab after the diagnosis is reached and will include billing, contacting the patient/referring physician, archiving and other tasks.So you have first to get?CAP's definition or elaborate your own one in accordance with your lab director.Ren? On Saturday, November 7, 2015 2:36 PM, Elaine allison Hoffman via Histonet wrote: Greeting everyone in Histo-land, I'm still working on a competency assessment procedure but I have a question that needs clarification.? According to CAP, "A laboratory must evaluate and document the competency of all testing personnel for each test system.? A TEST SYSTEM is the process that includes pre-analytic, analytic, and post-analytic steps used to produce a test result or set of results.? A test system may be manual, automated, multi-channel or single use and can include reagents, components, equipment or instruments required to produce results".? My question is, what histology tasks or steps are considered pre-analytic, analytic, and post-analytic?? In all the resources I've found, there seems to be a difference of opinion as to what is considered pre-analytic, analytic, and post-analytic specific to histology tasks which is not really considered "resulting" in histology. Also, there should be a different TEST SYSTEM for what pathologists do (resulting slides) and what PA's do (gross description of tissue), so what tasks are considered pre-analytic, analytic, and post-analytic specific for their jobs? A person could go crazy with all this and to ask an inspector, they aren't really sure either.? And different inspectors will give you different answers.Any takers out there? Elaine Hoffman ? ? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KSimeone at leavittmgt.com Mon Nov 9 08:45:40 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Mon, 9 Nov 2015 14:45:40 +0000 Subject: [Histonet] FULLTIME DAY/EVENING SHIFT (second shift) AND FULL TIME NIGHT SHIFT (IHC) POSITION DELRAY BCH FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C4D2DEA87@vm-email.leavittmgt.com> Hi Histonetters! We are looking for TWO full time licensed histotechs here in our very busy Delray Beach, Florida dermatology laboratory. These are permanent full time, (40 hours) position with benefits (medical/401k/vacation). Annual salary in the $50k range (NIGHT SHIFT DIFF AVAILABLE). THIS IS A DRUG FREE WORKPLACE. Background check, personality and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! DAY/EVENING (2nd SHIFT) POSTION: *full time position Mon-Fri 11a-7:30p (start time may vary by an hour) *MUST be licensed as a FLORIDA HISTOTECHNICIAN OR HISTOTECHNOLOGIST (NO pending licensure pls) *PREFER experience but WILL TRAIN a knowledgeable, willing candidate *duties to include (but not limited to): grossing, microtomy, accessioning, embedding and general histology *must be self motivated, reliable and a team player DAY/EVENING (2nd shift) SHIFT APPLICANT, PLEASE VISIT THIS LINK TO APPLY: https://advancedderm.applicantpro.com/jobs/240090.html NIGHT POSITION: *Full time position Mon-Fri 10:30p-7:00a (IMMUNOHISTOCHEMISTRY/SPECIALS department) *MUST be licensed as a FLORIDA HISTOTECHNOLOGIST (this is NOT negotiable) *EXPERIENCE WITH IHC A MUST! Leica (BOND) and Roche/Ventana equipment experience preferred *Must be able to multi-task special stains *MUST have at LEAST 2 years experience. Please DO NOT respond if you do not possess EXPERIENCE in this area! *must be confidant, quick learner, self motivated, reliable and a team player NIGHT SHIFT APPLICANT (IHC), PLEASE VISIT THIS LINK TO APPLY: https://advancedderm.applicantpro.com/jobs/278020.html Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From spencer at prometheushealthcare.com Mon Nov 9 14:16:19 2015 From: spencer at prometheushealthcare.com (Spencer Williams) Date: Mon, 9 Nov 2015 15:16:19 -0500 Subject: [Histonet] Spencer From Prometheus Healthcare - Recruiter Message-ID: <00ad01d11b2b$7f66c400$7e344c00$@prometheushealthcare.com> Hi there, My name is Spencer Williams, Recruiter with Prometheus healthcare. We specialize in the recruitment and placement of lab professionals. We are currently recruiting for a well known laboratory in both the Chicago and Northern California area. We are assisting them in finding top Histotechnician candidates for their laboratory. The position is a full time, permanent opportunity. If you may be interested in this opportunity, I'd love to discuss it further with you. Please feel free to reach out to me at any of my contacts below. Thank you in advance! Spencer Williams Recruiter Prometheus Healthcare Direct Line (407) 961-0186 Office (866) 857-1434 Fax (301) 368-2478 Spencer at prometheushealthcare.com www.prometheushealthcare.com From jennjennhisto at gmail.com Mon Nov 9 14:23:07 2015 From: jennjennhisto at gmail.com (Jennifer Robinson) Date: Mon, 9 Nov 2015 12:23:07 -0800 Subject: [Histonet] Shelf life? Message-ID: Can anyone tell me what the average shelf life is for a special stain kit (in general) associated with the Ventana Nexus? Thank you for your time. From jvickroy at SpringfieldClinic.com Mon Nov 9 14:46:54 2015 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Mon, 9 Nov 2015 20:46:54 +0000 Subject: [Histonet] On-the-job-training of histotechnicians Message-ID: <9B1A1501A800064397369BD8072E6BCA064B3AE8@E2K10DB.springfieldclinic.com> Currently we are training new staff members that each have a Bachelor of Science in Biology. According to the ASCP they can enroll to take the HT certification examination after a one-year period of on-the-job training. Like in the past we have purchased Freida Carson's text, Histotechnology: A Self-Instructional Text, and the workbook. We are also using Carson's BOC Study Guide - Histotechnology Certification Examinations, and the ASCP Histotech flash cards. I know that staff can successfully study this material on their own and can pass the HT certification because three of my past staff have already done this. However I am looking for additional help, plans, a syllabus, curriculum guide, etc. to help facilitate the training of these new staff members. They have quickly caught on to the practical skills of microtomy, embedding, and grossing however I am worried that we are not helping them to get the academic information that they need for certification. As I previously said I know that a person can get the certification by studying on their own but I also know that we are giving these people less time and instruction in the workplace. They are busy helping with the daily workload and therefore any studying of the academic material is having to be done on their own time. Any suggestions how to help these people without paying or having them enroll in an online Histotechnology program? Jim Vickroy BS, HT(ASCP) Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From Natacha.Mitchell at harrishealth.org Mon Nov 9 14:50:34 2015 From: Natacha.Mitchell at harrishealth.org (Mitchell, Natacha A) Date: Mon, 9 Nov 2015 20:50:34 +0000 Subject: [Histonet] IHC Staining Platforms Pros/Cons Message-ID: <6441A38AD60C3543B5861E7E678D0ABFBDE43A@hhmsg02.hchd.local> Hello fellow Histonetters, I was wondering if anyone would be willing to share their input regarding the differences, pros/cons and experiences with the Leica Bond III, Ultra Benchmark and Dako Omnis IHC staining platforms. Currently, we have two Ultra Benchmark strainers and use all prediluted Ab's. We do not stain for any type of research and we can have up to three runs per machine per day. We perform both IHC and ISH. Thank you all so much for your time and insight!!!! Natacha Mitchell, BS, HTL (ASCP) Histology Lab Supervisor Harris Health System Ben Taub General Hospital 1504 Taub Loop Houston, Tx, 77030 Office 713-873-0750 Lab 713-873-3258 Fax 713-873-3214 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From LRaff at uropartners.com Mon Nov 9 14:51:57 2015 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 9 Nov 2015 20:51:57 +0000 Subject: [Histonet] Opinion please Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B4E0F57@COLOEXCH01.uropartners.local> 'netters- As some of you have noted, I have been including links to a lifestyle blog with occasional lab elements that I have been writing in ChicagoNow. I haveobtained quite a few readers from here, but today received the first objection to these posts. This listserve has a history of "Friday Fun" and other light hearted elements, but if a significant number of you feel my inclusions are inappropriate, please let me know and I will discontinue them. Sincerely, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From LRaff at uropartners.com Tue Nov 10 10:44:03 2015 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 10 Nov 2015 16:44:03 +0000 Subject: [Histonet] Non-work related blog post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B4E2A66@COLOEXCH01.uropartners.local> The results are in from the decidedly unscientific poll taken over the last day and virtually all responders said to keep the blog links going. One request, a sensible one, was to indicate in the email subject line that the post is non-work related and thus easy to ignore or delete. I will do that from now on, thanks for the advice. Also, just in case these email notifications would have to be discontinued, I recommend that if you enjoy the blog and want to keep reading that you follow the instructions on the site and subscribe, or send me an email and I will add you to a mailing list notification system separate from the net list. Here is the latest post. It is a change of pace, but gotta keep things fresh. http://www.chicagonow.com/downsize-maybe/2015/11/first-doctors-without-borders-now-pathologists-without-houses/ Thanks for reading. Feedback always welcome. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From wyffels.jen at gmail.com Tue Nov 10 12:40:18 2015 From: wyffels.jen at gmail.com (Jennifer Wyffels) Date: Tue, 10 Nov 2015 13:40:18 -0500 Subject: [Histonet] Microm 355 series glass knife holder needed Message-ID: I?m looking for a glass knife holder for a Microm HM355E to assist with sectioning immunobed for a project. Price for a new knife holder is several thousand dollars and I don?t have grant monies available for purchasing. If anyone has a holder that currently is in retirement or underutilized that they would be willing to loan please let me know. Thanks in advance, Jen From gayle.callis at bresnan.net Tue Nov 10 13:57:31 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Tue, 10 Nov 2015 12:57:31 -0700 Subject: [Histonet] Glass knife holder Message-ID: <000101d11bf2$097e1af0$1c7a50d0$@bresnan.net> Hi Jen, If you can't find a glass knife holder for your microtome, try using a disposable blade, low profile. I know Linda Jenkins sectioned GMA embedded tissue (Immunobed is a formulation of GMA) with a disposable blade, but the microtome has to be powerful enough to do the work. If I remember correctly, she used Sakura Finetek's AccuEdge low profile and very sharp, but not the same microtome as yours. It would certainly be worth a try with whatever disposable blade you have in your lab before investing in the expensive holder. You will be picking each section off the blade one at a time with a fine forceps, and then float it on RT distilled water. A glass staining dish has always worked for this. Good luck and hope you have some success. Gayle Callis HTL/HT/MT(ASCP) From choose4health at yahoo.com Tue Nov 10 20:34:55 2015 From: choose4health at yahoo.com (Nan Gray) Date: Tue, 10 Nov 2015 18:34:55 -0800 Subject: [Histonet] Please unsubscribe me Message-ID: Sent from my iPhone From jerrysedgewick at gmail.com Wed Nov 11 08:20:15 2015 From: jerrysedgewick at gmail.com (J. Sedgewick) Date: Wed, 11 Nov 2015 08:20:15 -0600 Subject: [Histonet] cells on medical devices Message-ID: <6BA0BEF4B02245FC80AF303B894D7BCA@sedge> I?m wondering how long cells stay intact on a medical device when packaged with dry ice. Any experience with that? Jerry Sedgewick Imaging and Analysis, LLC From llang at aipathology.com Wed Nov 11 10:53:59 2015 From: llang at aipathology.com (LeAnn Lang) Date: Wed, 11 Nov 2015 16:53:59 +0000 Subject: [Histonet] Open Histology Position Message-ID: Associates in Pathology in Wausau, Wisconsin currently has an full time open position for a histotechnician or histotechnologist. Interested candidates should send their resumes to llang at aipathology.com Thank you, LeAnn <>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<>*<> LeAnn Lang Associates in Pathology Practice Administrator Phone: 715-847-0075 (ext 50259) llang at aipathology.com From Toni.Rathborne at rwjuh.edu Wed Nov 11 11:00:36 2015 From: Toni.Rathborne at rwjuh.edu (Rathborne, Toni) Date: Wed, 11 Nov 2015 17:00:36 +0000 Subject: [Histonet] OR specimens in formalin Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F243329B0DB@vap1014.win.rwjuh.edu> Good afternoon. This question is mainly for those who attended NSH this year, but others may know what I'm looking for too. In the Exhibition Hall, there was a product which was to be used in the OR and in which the specimen was placed in a bag, which itself was in an instrument and was then filled with formalin depending on the size of the specimen. Although I didn't think that we had a need for it at the time, there has been some interest expressed by the OR now. If anyone remembers the name of this instrument or the company that sells it, I'd be grateful if you could respond. Toni From melissa at alliedsearchpartners.com Wed Nov 11 11:22:29 2015 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Wed, 11 Nov 2015 17:22:29 +0000 Subject: [Histonet] Pathology Assistant/Grossing Job Opening in New York Message-ID: Hello All, I have a job opening in the Port Chester, NY Area. Looking for a Pathology Assistant/Grossing Tech for a Full Time Tuesday-Saturday 1am-9:30am position. Must have at least a Bachelor's Degree and Grossing experience. Please contact me for more details. Thanks! Melissa Owens President, Laboratory Staffing Allied Search Partners www.linkedin.com/in/melissaphelan/ http://www.alliedsearchpartners.com T: 888.388.7571 ext. 102 F: 888.388.7572 From pedro.louro at rutgers.edu Wed Nov 11 11:31:37 2015 From: pedro.louro at rutgers.edu (Pedro Louro) Date: Wed, 11 Nov 2015 12:31:37 -0500 (EST) Subject: [Histonet] GMS silver stain on frozen tissue In-Reply-To: <252264706.50486975.1447262790606.JavaMail.zimbra@vpr.rutgers.edu> Message-ID: <835649836.50492829.1447263097369.JavaMail.zimbra@vpr.rutgers.edu> Hi everyone, Has anyone experimented with performing a Grocott Methenamine Silver stain on frozen tissues? I have a PI that is requesting this but I cannot find any literature on frozen tissue. Any help or ideas is appreciated. Thanks in advance, Pedro From Toni.Rathborne at rwjuh.edu Wed Nov 11 14:09:15 2015 From: Toni.Rathborne at rwjuh.edu (Rathborne, Toni) Date: Wed, 11 Nov 2015 20:09:15 +0000 Subject: [Histonet] OR specimens in formalin In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F243329B0DB@vap1014.win.rwjuh.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F243329B0DB@vap1014.win.rwjuh.edu> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F243329C1EF@vap1014.win.rwjuh.edu> Thanks everyone for your quick responses! The product I was looking for was SealSAFE by Milestone. Toni _____________________________________________ From: Rathborne, Toni Sent: Wednesday, November 11, 2015 12:01 PM To: histonet at lists.utsouthwestern.edu Subject: OR specimens in formalin Good afternoon. This question is mainly for those who attended NSH this year, but others may know what I'm looking for too. In the Exhibition Hall, there was a product which was to be used in the OR and in which the specimen was placed in a bag, which itself was in an instrument and was then filled with formalin depending on the size of the specimen. Although I didn't think that we had a need for it at the time, there has been some interest expressed by the OR now. If anyone remembers the name of this instrument or the company that sells it, I'd be grateful if you could respond. Toni From AJohnson at aipathology.com Thu Nov 12 06:54:31 2015 From: AJohnson at aipathology.com (Amy Johnson) Date: Thu, 12 Nov 2015 12:54:31 +0000 Subject: [Histonet] Hazard Communication Standard Labels Message-ID: Most chemical should be coming from the manufacturers with all the new requirements, i.e. Pictograms, signal words, hazard and precautionary statements, product identifiers and supplier identification. What is everyone doing to get this same information onto secondary bottles? Thanks for your help Amylin Johnson, HTL(ASCP) Associates in Pathology Wausau WI 715-847-2130 From LRaff at uropartners.com Thu Nov 12 08:04:46 2015 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 12 Nov 2015 14:04:46 +0000 Subject: [Histonet] Blog Post-non work related Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B4E5366@COLOEXCH01.uropartners.local> Love and great food,,,come to Chicago and find both. http://www.chicagonow.com/downsize-maybe/2015/11/food-for-thought-37-years-of-a-great-wife-and-great-chicago-restaurants/ or tinyurl.com/down1112 Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From Nancy_Schmitt at pa-ucl.com Thu Nov 12 09:26:54 2015 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Thu, 12 Nov 2015 15:26:54 +0000 Subject: [Histonet] toluidine blue Message-ID: <12b3e3b9d94846f2b16d529a0d072079@mercury.wad.pa-ucl.com> Hi All- Is anyone using Toluidine Blue on air dried touch prep slides? Specifically for endobronchial ultra sound specimens (ebus). I am wondering if you can tell me what you are using for a toluidine blue recipe. Thank you for your help- Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager United Clinical Laboratories Dubuque, IA 52001 Ph. 563-690-4142 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From hhawkins at UTMB.EDU Thu Nov 12 09:49:39 2015 From: hhawkins at UTMB.EDU (Hawkins, Hal K.) Date: Thu, 12 Nov 2015 15:49:39 +0000 Subject: [Histonet] GMS silver stain on frozen tissue In-Reply-To: <835649836.50492829.1447263097369.JavaMail.zimbra@vpr.rutgers.edu> References: <252264706.50486975.1447262790606.JavaMail.zimbra@vpr.rutgers.edu>, <835649836.50492829.1447263097369.JavaMail.zimbra@vpr.rutgers.edu> Message-ID: <22624908330375439D6382C9F95093FF581EA971@GRMBX1.utmb.edu> We do this routinely at the Shriners Burns Hospital in Galveston when asked to look for fungi in infected burn wounds and provide a "rapid section" report. It works very well when the timing is carefully controlled by frequent visual inspection. We use a paraffin section as a control. We also run a tissue Gram stain on these samples and report the results in about two hours. ________________________________________ From: Pedro Louro via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, November 11, 2015 11:31 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] GMS silver stain on frozen tissue Hi everyone, Has anyone experimented with performing a Grocott Methenamine Silver stain on frozen tissues? I have a PI that is requesting this but I cannot find any literature on frozen tissue. Any help or ideas is appreciated. Thanks in advance, Pedro _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence at grhs.net Thu Nov 12 09:49:51 2015 From: mpence at grhs.net (Mike Pence) Date: Thu, 12 Nov 2015 15:49:51 +0000 Subject: [Histonet] Slide and cassette printers Message-ID: Okay, I am reaching out to the people that I know that has an answer to everything. What system are you using to print slides and cassettes from in your lab? Is it intergraded with your LIS and if so what LIS are you using? I am not looking for a system that prints to labels, I what it to print directly to the slide or cassette. Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 mpence at grhs.net | www.greatrivermedical.org. www.Facebook.com/GreatRiverHealthSystems | www.Twitter/GreatRiverMed Information in this communication, including attachments, is confidential and intended only for the addressee(s). This communication may contain privileged, confidential, proprietary or trade secret information entitled to protection or exemption from disclosure under law. If you are not an intended recipient, please know that any use, distribution or copying of this communication, or any action taken based on the information in this communication, is unauthorized and may be unlawful. If you received this communication in error, please notify the sender and delete this communication from your device. From dunatrsd at sbcglobal.net Thu Nov 12 10:26:37 2015 From: dunatrsd at sbcglobal.net (dusko trajkovic) Date: Thu, 12 Nov 2015 16:26:37 +0000 (UTC) Subject: [Histonet] CD16/FCGR4 Antibody-Mouse specific References: <642560077.3127794.1447345597872.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <642560077.3127794.1447345597872.JavaMail.yahoo@mail.yahoo.com> Hello Histonetters,Does anyone have any experience with this antibody? Any information, pos or neg, would be greatly appreciated.Have a good day.thanksDusko Trajkovic From vapatpxs at yahoo.com Thu Nov 12 10:46:07 2015 From: vapatpxs at yahoo.com (Va Paula Sicurello) Date: Thu, 12 Nov 2015 16:46:07 +0000 (UTC) Subject: [Histonet] Test email References: <1750652452.3484753.1447346767121.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1750652452.3484753.1447346767121.JavaMail.yahoo@mail.yahoo.com> Good Morning Netters, This is a test to see if I can get an email through using this email account. My posts haven't been making it through and I know you miss me. Sincerely, Paula Paula Sicurello Histotechnology Specialist UC San Diego Health From histology81176 at att.net Thu Nov 12 11:16:58 2015 From: histology81176 at att.net (Histology Technician) Date: Thu, 12 Nov 2015 17:16:58 +0000 (UTC) Subject: [Histonet] Do you check humidity and room temp for your daily QC in the lab? References: <754894842.3181996.1447348618531.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <754894842.3181996.1447348618531.JavaMail.yahoo@mail.yahoo.com> Does anyone check humidity and room temp as your QC?? If so, can you share you procedure and QC form?? Do you use a company or do you monitor it yourself? Thanks! From liz at premierlab.com Thu Nov 12 11:40:34 2015 From: liz at premierlab.com (Elizabeth Chlipala) Date: Thu, 12 Nov 2015 10:40:34 -0700 Subject: [Histonet] Do you check humidity and room temp for your daily QC in the lab? In-Reply-To: <754894842.3181996.1447348618531.JavaMail.yahoo@mail.yahoo.com> References: <754894842.3181996.1447348618531.JavaMail.yahoo.ref@mail.yahoo.com> <754894842.3181996.1447348618531.JavaMail.yahoo@mail.yahoo.com> Message-ID: <14E2C6176416974295479C64A11CB9AE02851F9CF02A@SBS2K8.premierlab.local> We do both, we only have criteria for the temp but we do record both in all rooms in the laboratory. We use traceable wall thermometers/humidity units and replace upon expiration. We have a few SOP's that govern this, we are a GLP compliant lab. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Histology Technician via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, November 12, 2015 10:17 AM To: Histonet List Subject: [Histonet] Do you check humidity and room temp for your daily QC in the lab? Does anyone check humidity and room temp as your QC?? If so, can you share you procedure and QC form?? Do you use a company or do you monitor it yourself? Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock at mclean.harvard.edu Thu Nov 12 11:52:04 2015 From: twheelock at mclean.harvard.edu (Wheelock, Timothy R.) Date: Thu, 12 Nov 2015 17:52:04 +0000 Subject: [Histonet] Temperature and Humidity check Message-ID: <69718C0B0B3C414D9F8E7214AD400CC997DCB161@PHSX10MB11.partners.org> Hi: Yes, I check both temperature and humidity as part of a morning " lab systems check". This also includes checking the fume hoods, distilled water, emergency eye wash, refrigerator and oven temperatures, the tissue processor clock, and embedding center and water bath temperatures. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont Center The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From vapatpxs at yahoo.com Thu Nov 12 18:23:26 2015 From: vapatpxs at yahoo.com (Va Paula Sicurello) Date: Fri, 13 Nov 2015 00:23:26 +0000 (UTC) Subject: [Histonet] Histology Related Questions? References: <1866036549.3744596.1447374206500.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1866036549.3744596.1447374206500.JavaMail.yahoo@mail.yahoo.com> Hello Netters, I am taking a research techniques class for my MBA this term and need to have a project that I can ask quantifiable questions. ?I was thinking about productivity and benchmarks in the Histology and IHC labs. If I send an email with my questions, will you be kind and answer them in a reply email? I hope to keep it between 15-20 questions. I even promise to share my results once I compile all the information. Suggested questions are also gratefully accepted. Toodles!?Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health From vapatpxs at yahoo.com Thu Nov 12 18:26:11 2015 From: vapatpxs at yahoo.com (Va Paula Sicurello) Date: Fri, 13 Nov 2015 00:26:11 +0000 (UTC) Subject: [Histonet] Histology Related Questions? References: <1777262438.3706705.1447374371520.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <1777262438.3706705.1447374371520.JavaMail.yahoo@mail.yahoo.com> Hello Netters, I am taking a research techniques class for my MBA this term and need to have a project that I can ask quantifiable questions. ?I was thinking about productivity and benchmarks in the Histology and IHC labs. If I send an email with my questions, will you be kind and answer them in a reply email? I hope to keep it between 15-20 questions. I even promise to share my results once I compile all the information. Suggested questions are also gratefully accepted. Toodles!?Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health From rjbuesa at yahoo.com Fri Nov 13 08:06:54 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 13 Nov 2015 14:06:54 +0000 (UTC) Subject: [Histonet] Histology Related Questions? In-Reply-To: <1777262438.3706705.1447374371520.JavaMail.yahoo@mail.yahoo.com> References: <1777262438.3706705.1447374371520.JavaMail.yahoo@mail.yahoo.com> Message-ID: <2032210108.3288257.1447423614231.JavaMail.yahoo@mail.yahoo.com> That is OK with me.Ren? On Thursday, November 12, 2015 7:48 PM, Va Paula Sicurello via Histonet wrote: Hello Netters, I am taking a research techniques class for my MBA this term and need to have a project that I can ask quantifiable questions. ?I was thinking about productivity and benchmarks in the Histology and IHC labs. If I send an email with my questions, will you be kind and answer them in a reply email? I hope to keep it between 15-20 questions. I even promise to share my results once I compile all the information. Suggested questions are also gratefully accepted. Toodles!?Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kstoll at mcw.edu Fri Nov 13 08:29:24 2015 From: kstoll at mcw.edu (Stoll, Kathryn) Date: Fri, 13 Nov 2015 14:29:24 +0000 Subject: [Histonet] IHC on old slides Message-ID: Hi Everyone, I have been doing some IHC staining on slides that are 20 years old or more. Depending on the antibody the staining is pale in comparison to my control slide. Does anyone have experience with staining older slides? Do you have any tips to share? The slide storage conditions are not something I can control. I am under the assumption that they were stored at room temperature. Thank you in advance. Kathryn Stoll From rjbuesa at yahoo.com Fri Nov 13 10:06:25 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 13 Nov 2015 16:06:25 +0000 (UTC) Subject: [Histonet] IHC on old slides In-Reply-To: References: Message-ID: <1796325472.3609488.1447430785170.JavaMail.yahoo@mail.yahoo.com> The only thing you can do is to prolong the Ab staining time but, in order to avoid excessive background, dilute it. You will have to find an adequate balance between a more diluted Ab with a weaker spitope signal and a? prolonged incubation time and there is no "magic formula" for obtaining it. You will have to make tests.Antigen in stored slides oxidizes?producing a weaker signal.Ren?? On Friday, November 13, 2015 10:51 AM, "Stoll, Kathryn via Histonet" wrote: Hi Everyone, I have been doing some IHC staining on slides that are 20 years old or more.? Depending on the antibody the staining is pale in comparison to my control slide.? Does anyone have experience with staining older slides?? Do you have any tips to share?? The slide storage conditions are not something I can control.? I am under the assumption that they were stored at room temperature. Thank you in advance. Kathryn Stoll _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From angelapatriciay at gmail.com Fri Nov 13 12:23:56 2015 From: angelapatriciay at gmail.com (Angela Yepes) Date: Fri, 13 Nov 2015 13:23:56 -0500 Subject: [Histonet] Mohs sections References: <1974EFE1-CAC7-4203-B2CE-54C6E78AE12A@gmail.com> Message-ID: <9AB1BDCC-37FF-497D-A479-B394A8D5FAE8@gmail.com> > From: Angela Yepes > Date: November 12, 2015 at 6:05:57 PM EST > To: histonet at lists.utsouthwestern.edu > Subject: Mohs sections > > >> From: Angela Yepes >> Date: November 12, 2015 at 4:15:50 PM EST >> To: histonet-request at lists.utsouthwestern.edu >> Subject: Mohs sections >> Hello everyone: >> While cutting Sections for Mohs surgery I noticed the tissue detaching from the O.C.T. Embedding medium and subsequent rolling of the tissue. >> To correct the problem the chuck was rotated to allow cutting from the opposite side (epidermis side). This maneuver did not correct the problem. I tried to unroll the edges with stiff brush which seemed to correct the problem, but it did not. I only found out the sections were still rolling up after the staining. >> The cryostat temperature was -23C and the sections thickness was -14 microns >> Please see pictures. >> Please help >> >> >> >> >> Sent from my iPhone >> From carl.hobbs at kcl.ac.uk Fri Nov 13 13:25:04 2015 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 13 Nov 2015 19:25:04 +0000 Subject: [Histonet] IHC on old slides Message-ID: Amplification is the only way. Possibly, tyramide amplification will help. It will cost you to buy a kit...sure, make your own but....you have to weigh up pros/cons Sure....you will have to play with variables. From DEBORAH_ELLENBURG at bshsi.org Fri Nov 13 13:42:29 2015 From: DEBORAH_ELLENBURG at bshsi.org (Ellenburg, Deborah) Date: Fri, 13 Nov 2015 19:42:29 +0000 Subject: [Histonet] #ExtMail# Slide and cassette printers In-Reply-To: References: Message-ID: <66C85B18-BCE2-4F2D-9FF0-7136B605CE72@bshsi.org> We have the ShurMark slide and cassette engraver that interfaces with CoPath. Sent from my iPhone > On Nov 12, 2015, at 10:50 AM, Mike Pence via Histonet wrote: > > Okay, I am reaching out to the people that I know that has an answer to everything. What system are you using to print slides and cassettes from in your lab? Is it intergraded with your LIS and if so what LIS are you using? I am not looking for a system that prints to labels, I what it to print directly to the slide or cassette. > > Michael S. Pence | Supervisor of Laboratory Services > Great River Health Systems > 1221 S. Gear Ave. | West Burlington, IA 52655 > Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 > mpence at grhs.net | www.greatrivermedical.org. > www.Facebook.com/GreatRiverHealthSystems | www.Twitter/GreatRiverMed > > > Information in this communication, including attachments, is confidential and intended only for the addressee(s). This communication may contain privileged, confidential, proprietary or trade secret information entitled to protection or exemption from disclosure under law. If you are not an intended recipient, please know that any use, distribution or copying of this communication, or any action taken based on the information in this communication, is unauthorized and may be unlawful. If you received this communication in error, please notify the sender and delete this communication from your device. > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ From vapatpxs at yahoo.com Sat Nov 14 10:39:04 2015 From: vapatpxs at yahoo.com (Va Paula Sicurello) Date: Sat, 14 Nov 2015 16:39:04 +0000 (UTC) Subject: [Histonet] Histology Lab Survey Questions References: <970743067.4496622.1447519144079.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <970743067.4496622.1447519144079.JavaMail.yahoo@mail.yahoo.com> Hello Netters, Thank you for taking my survey. ?I will use your replies for my research project and I will also let everyone on the list know the results as well. >From what I've found, the surveys performed in the past asked similar questions but more at the institution level. ?I would like to get information at the individual level. ?There are 16 questions. Please reply to this email offline with your answers. Thank you for helping me. ?Let's hope I get an A! :-) Here we go:Questions aboutyou: 1.?????Education (highest achieved): a.??????High school b.?????Associates degree c.??????Bachelor?s degree d.?????Master?s degree e.??????PhD or MD 2.?????Certification: a.??????None b.?????HT c.??????HTL d.?????Other, please describe 3.?????How did you learn histology? a.??????On the job b.?????Histotechnology school 4.?????How many years of experience in histology? 5.?????How many blocks can you embed in an hour? 6.?????How many of the following can you produce in an 8 hourday??????????????????????????? ????????????????????(You can calculate from howmany of each task you can perform in one hour:?20 blocks with one slide each per hour = 160 blocks and 160 slides) a.??????Blocks b.?????Slides Questions aboutyour place of work: 7.?????How many blocks does your lab embed a day? 8.?????How many staff does your lab have? a.??????Technical (HT, HTL, etc.) b.?????Non-technical (lab assistants, etc.) 9.?????How is IHC handled in your lab?? a. Separate staff (if so how many?)? b. All staff perform 10.? Howmany IHC slides does your lab stain per day? 11.? Howmany of the following can your lab run? a.??????Antibodies (total number available for testing) b.?????Special Stains (total number available for testing) 12.? Doesyour lab use automation?? Yes or No Ifyes, please describe: 13.? Whoprepares the slides prior to release to the pathologist? 14.? Whofiles the blocks and slides? 15.? Whattype of institution do you work at? a.??????Hospital based Histology lab b.?????Clinic based Histology lab c.??????Independent lab (such as a referral lab) 16.? BonusQuestion:? Has your family stopped askingwhy you come home with blue or pink-orange fingers? ????????????????OPTIONAL: Please add your comments, suggestions,or thoughts. ?Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health From ian.bernard at comcast.net Sun Nov 15 20:44:14 2015 From: ian.bernard at comcast.net (ian bernard) Date: Sun, 15 Nov 2015 19:44:14 -0700 Subject: [Histonet] Pathology amended reports In-Reply-To: References: Message-ID: <004c01d12018$aeefcc70$0ccf6550$@comcast.net> For us an amended report is just that; it is an amended report for a particular reason, namely for typographical or other minor errors and/or diagnostic changes and/or disagreements. Additional and new information (further testing/consults) is treated with an addendum. Once the error or information is corrected in the report, we disclaim in the report why the report was amended and indicate that the provider or clinician was notified of the amendment on a particular date and time if the amendment was significant. No need to confused the final product report with initial errors. This can lead to other errors. IB -----Original Message----- From: STEVEN PINHEIRO via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, October 28, 2015 10:33 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pathology amended reports I realize this is a broad topic but I am trying to get a flavor for best practices. When you issue an amended report, is the original 'removed'? Unlike the practice on the clinical lab side of leaving the first errant test result in place and issuing a corrected result, pathology (at least here) has felt there are more down side issues with leaving the wrong information in the patient chart. Your specific thoughts on the actual protocol used for amended reports issued in your practice is appreciated. Thanks Steven Pinheiro, MBA, MLS(ASCP)DLMCM Manager Anatomic Pathology and Cytology Loyola University Medical Center 2160 S First Ave, Bldg 110 Rm 2214 Maywood, IL 60153 708-327-2642 (O) 708-327-2620 (F) spinheiro at lumc.edu "You must do the thing you think you cannot do" E. Roosevelt Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech at imagesbyhopper.com Mon Nov 16 04:46:19 2015 From: histotech at imagesbyhopper.com (histotech at imagesbyhopper.com) Date: Mon, 16 Nov 2015 05:46:19 -0500 Subject: [Histonet] Pathology amended reports In-Reply-To: <004c01d12018$aeefcc70$0ccf6550$@comcast.net> References: <004c01d12018$aeefcc70$0ccf6550$@comcast.net> Message-ID: We layer the amended/addended report information at the top of the printed/faxed report. We do not allow for changing the original report, as that is a legal document. Further, if an amendment or addendum is needed and the report is faxed (with the new information on top), there is no confusion on the part of the receiving personnel. We do require that these reports explain the reason for the change or addition. The EMR will show both the original and the amended/addended report contents on a separate tab. Best, Michelle On Nov 15, 2015, at 9:44 PM, ian bernard via Histonet wrote: For us an amended report is just that; it is an amended report for a particular reason, namely for typographical or other minor errors and/or diagnostic changes and/or disagreements. Additional and new information (further testing/consults) is treated with an addendum. Once the error or information is corrected in the report, we disclaim in the report why the report was amended and indicate that the provider or clinician was notified of the amendment on a particular date and time if the amendment was significant. No need to confused the final product report with initial errors. This can lead to other errors. IB -----Original Message----- From: STEVEN PINHEIRO via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, October 28, 2015 10:33 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pathology amended reports I realize this is a broad topic but I am trying to get a flavor for best practices. When you issue an amended report, is the original 'removed'? Unlike the practice on the clinical lab side of leaving the first errant test result in place and issuing a corrected result, pathology (at least here) has felt there are more down side issues with leaving the wrong information in the patient chart. Your specific thoughts on the actual protocol used for amended reports issued in your practice is appreciated. Thanks Steven Pinheiro, MBA, MLS(ASCP)DLMCM Manager Anatomic Pathology and Cytology Loyola University Medical Center 2160 S First Ave, Bldg 110 Rm 2214 Maywood, IL 60153 708-327-2642 (O) 708-327-2620 (F) spinheiro at lumc.edu "You must do the thing you think you cannot do" E. Roosevelt Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From boneimage8 at gmail.com Mon Nov 16 08:16:36 2015 From: boneimage8 at gmail.com (Marc DeCarlo) Date: Mon, 16 Nov 2015 08:16:36 -0600 Subject: [Histonet] North Central Instruments Message-ID: Hope everyone is having a great start to the week. Does anyone have a direct number or email address for someone at North Central Instruments? The main number and generic email from their website has not gotten our messages returned. Thank you in advance, Marc DeCarlo From nexgenpath at gmail.com Mon Nov 16 09:48:13 2015 From: nexgenpath at gmail.com (Nexgen Pathology) Date: Mon, 16 Nov 2015 11:48:13 -0400 Subject: [Histonet] Histonet Digest, Vol 144, Issue 14 In-Reply-To: References: Message-ID: Hi, im a budding histotechnician at Nexgen Pathology Limited in Trinidad and Tobago. What are your protocols with respect to taking levels for biopsies? How many levels do you usually take per biopsy? Email me personally if you can @Nicholas009 at gmail.com Thanks Alot Nicholas Seepersad *Nexgen Pathology* 6 Eastern Main Rd. San Juan Trinidad & Tobago Tel./Fax. (868) 674-7284 nexgenpathology.com On Sat, Nov 14, 2015 at 2:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Mohs sections (Angela Yepes) > 2. Re: IHC on old slides (Hobbs, Carl) > 3. Re: #ExtMail# Slide and cassette printers (Ellenburg, Deborah) > 4. Histology Lab Survey Questions (Va Paula Sicurello) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 13 Nov 2015 13:23:56 -0500 > From: Angela Yepes > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Mohs sections > Message-ID: <9AB1BDCC-37FF-497D-A479-B394A8D5FAE8 at gmail.com> > Content-Type: text/plain; charset=us-ascii > > > > > From: Angela Yepes > > Date: November 12, 2015 at 6:05:57 PM EST > > To: histonet at lists.utsouthwestern.edu > > Subject: Mohs sections > > > > > >> From: Angela Yepes > >> Date: November 12, 2015 at 4:15:50 PM EST > >> To: histonet-request at lists.utsouthwestern.edu > >> Subject: Mohs sections > >> Hello everyone: > >> While cutting Sections for Mohs surgery I noticed the tissue detaching > from the O.C.T. Embedding medium and subsequent rolling of the tissue. > >> To correct the problem the chuck was rotated to allow cutting from the > opposite side (epidermis side). This maneuver did not correct the problem. > I tried to unroll the edges with stiff brush which seemed to correct the > problem, but it did not. I only found out the sections were still rolling > up after the staining. > >> The cryostat temperature was -23C and the sections thickness was -14 > microns > >> Please see pictures. > >> Please help > >> > >> > >> > >> > >> Sent from my iPhone > >> > > > ------------------------------ > > Message: 2 > Date: Fri, 13 Nov 2015 19:25:04 +0000 > From: "Hobbs, Carl" > To: histonet > Subject: Re: [Histonet] IHC on old slides > Message-ID: > < > VI1PR03MB1407C1C22A150F0A81DC6F04C4110 at VI1PR03MB1407.eurprd03.prod.outlook.com > > > > Content-Type: text/plain; charset="iso-8859-1" > > Amplification is the only way. > > Possibly, tyramide amplification will help. > > It will cost you to buy a kit...sure, make your own but....you have to > weigh up pros/cons > > > Sure....you will have to play with variables. > > > > ------------------------------ > > Message: 3 > Date: Fri, 13 Nov 2015 19:42:29 +0000 > From: "Ellenburg, Deborah" > To: Mike Pence > Cc: "histonet at lists.utsouthwestern.edu" > , > "histonet-bounces at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] #ExtMail# Slide and cassette printers > Message-ID: <66C85B18-BCE2-4F2D-9FF0-7136B605CE72 at bshsi.org> > Content-Type: text/plain; charset="us-ascii" > > We have the ShurMark slide and cassette engraver that interfaces with > CoPath. > > Sent from my iPhone > > > On Nov 12, 2015, at 10:50 AM, Mike Pence via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > > Okay, I am reaching out to the people that I know that has an answer to > everything. What system are you using to print slides and cassettes from in > your lab? Is it intergraded with your LIS and if so what LIS are you using? > I am not looking for a system that prints to labels, I what it to print > directly to the slide or cassette. > > > > Michael S. Pence | Supervisor of Laboratory Services > > Great River Health Systems > > 1221 S. Gear Ave. | West Burlington, IA 52655 > > Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 > > mpence at grhs.net | www.greatrivermedical.org< > http://www.greatrivermedical.org>. > > www.Facebook.com/GreatRiverHealthSystems< > http://www.Facebook.com/GreatRiverHealthSystems> | > www.Twitter/GreatRiverMed > > > > > > Information in this communication, including attachments, is > confidential and intended only for the addressee(s). This communication may > contain privileged, confidential, proprietary or trade secret information > entitled to protection or exemption from disclosure under law. If you are > not an intended recipient, please know that any use, distribution or > copying of this communication, or any action taken based on the information > in this communication, is unauthorized and may be unlawful. If you received > this communication in error, please notify the sender and delete this > communication from your device. > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ________________________________________________________________________________________________________ > The information in this communication is intended to be confidential to > the Individual(s) and/or Entity to whom it is addressed. > It may contain information of a Privileged and/or Confidential nature, > which is subject to Federal and/or State privacy regulations. > In the event that you are not the intended recipient or the agent of the > intended recipient, do not copy or use the information > contained within this communication, or allow it to be read, copied or > utilized in any manner, by any other person(s). Should > this communication be received in error, please notify the sender > immediately either by response e-mail or by phone, > and permanently delete the original e-mail, attachment(s), and any copies. > > ________________________________________________________________________________________________________ > > > > ------------------------------ > > Message: 4 > Date: Sat, 14 Nov 2015 16:39:04 +0000 (UTC) > From: Va Paula Sicurello > To: "histonet at lists.utsouthwestern.edu Histonet" > > Subject: [Histonet] Histology Lab Survey Questions > Message-ID: > <970743067.4496622.1447519144079.JavaMail.yahoo at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > Hello Netters, > Thank you for taking my survey. ?I will use your replies for my research > project and I will also let everyone on the list know the results as well. > >From what I've found, the surveys performed in the past asked similar > questions but more at the institution level. ?I would like to get > information at the individual level. ?There are 16 questions. > Please reply to this email offline with your answers. > Thank you for helping me. ?Let's hope I get an A! :-) > Here we go:Questions aboutyou: > > 1.?????Education (highest achieved): > > a.??????High school > > b.?????Associates degree > > c.??????Bachelor?s degree > > d.?????Master?s degree > > e.??????PhD or MD > > 2.?????Certification: > > a.??????None > > b.?????HT > > c.??????HTL > > d.?????Other, please describe > > 3.?????How did you learn histology? > > a.??????On the job > > b.?????Histotechnology school > > 4.?????How many years of experience in histology? > > 5.?????How many blocks can you embed in an hour? > > 6.?????How many of the following can you produce in an 8 > hourday??????????????????????????? ????????????????????(You can calculate > from howmany of each task you can perform in one hour:?20 blocks with one > slide each per hour = 160 blocks and 160 slides) > > a.??????Blocks > > b.?????Slides > > > Questions aboutyour place of work: > > 7.?????How many blocks does your lab embed a day? > > 8.?????How many staff does your lab have? > > a.??????Technical (HT, HTL, etc.) > > b.?????Non-technical (lab assistants, etc.) > > 9.?????How is IHC handled in your lab?? > > a. Separate staff (if so how many?)? > > b. All staff perform > > 10.? Howmany IHC slides does your lab stain per day? > > 11.? Howmany of the following can your lab run? > > a.??????Antibodies (total number available for testing) > > b.?????Special Stains (total number available for testing) > > 12.? Doesyour lab use automation?? Yes or No > > Ifyes, please describe: > > 13.? Whoprepares the slides prior to release to the pathologist? > > 14.? Whofiles the blocks and slides? > > 15.? Whattype of institution do you work at? > > a.??????Hospital based Histology lab > > b.?????Clinic based Histology lab > > c.??????Independent lab (such as a referral lab) > > 16.? BonusQuestion:? Has your family stopped askingwhy you come home with > blue or pink-orange fingers? > > > ????????????????OPTIONAL: Please add your comments, suggestions,or > thoughts. > ?Sincerely, > Paula > Paula SicurelloHistotechnology SpecialistUC San Diego Health > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 144, Issue 14 > ***************************************** > From rjbuesa at yahoo.com Mon Nov 16 11:19:51 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 16 Nov 2015 17:19:51 +0000 (UTC) Subject: [Histonet] Histonet Digest, Vol 144, Issue 14 In-Reply-To: References: Message-ID: <1836969495.1241485.1447694391250.JavaMail.yahoo@mail.yahoo.com> That is a question completely dependent on your pathologist. S/he is the one taking those decisions.Ren? On Monday, November 16, 2015 11:04 AM, Nexgen Pathology via Histonet wrote: Hi, im a budding histotechnician at Nexgen Pathology Limited in Trinidad and Tobago. What are your protocols with respect to taking levels for biopsies? How many levels do you usually take per biopsy? Email me personally if you can @Nicholas009 at gmail.com Thanks Alot Nicholas Seepersad *Nexgen Pathology* 6 Eastern Main Rd. San Juan Trinidad & Tobago Tel./Fax. (868) 674-7284 nexgenpathology.com On Sat, Nov 14, 2015 at 2:00 PM, wrote: > Send Histonet mailing list submissions to >? ? ? ? histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit >? ? ? ? http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to >? ? ? ? histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at >? ? ? ? histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > >? ? 1. Mohs sections (Angela Yepes) >? ? 2. Re: IHC on old slides (Hobbs, Carl) >? ? 3. Re: #ExtMail#? Slide and cassette printers (Ellenburg, Deborah) >? ? 4. Histology Lab Survey Questions (Va Paula Sicurello) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 13 Nov 2015 13:23:56 -0500 > From: Angela Yepes > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Mohs sections > Message-ID: <9AB1BDCC-37FF-497D-A479-B394A8D5FAE8 at gmail.com> > Content-Type: text/plain;? ? ? charset=us-ascii > > > > > From: Angela Yepes > > Date: November 12, 2015 at 6:05:57 PM EST > > To: histonet at lists.utsouthwestern.edu > > Subject: Mohs sections > > > > > >> From: Angela Yepes > >> Date: November 12, 2015 at 4:15:50 PM EST > >> To: histonet-request at lists.utsouthwestern.edu > >> Subject: Mohs sections > >> Hello everyone: > >> While cutting Sections for Mohs surgery I noticed the tissue detaching > from the O.C.T. Embedding medium? and subsequent rolling of the tissue. > >> To correct the problem the chuck was rotated to allow cutting from the > opposite side (epidermis side). This maneuver did not correct the problem. > I tried to unroll the edges with stiff brush which seemed to correct the > problem, but it did not. I only? found out the sections were still rolling > up after the staining. > >> The cryostat temperature was -23C and the sections thickness was -14 > microns > >> Please see pictures. > >> Please help > >> > >> > >> > >> > >> Sent from my iPhone > >> > > > ------------------------------ > > Message: 2 > Date: Fri, 13 Nov 2015 19:25:04 +0000 > From: "Hobbs, Carl" > To: histonet > Subject: Re: [Histonet] IHC on old slides > Message-ID: >? ? ? ? < > VI1PR03MB1407C1C22A150F0A81DC6F04C4110 at VI1PR03MB1407.eurprd03.prod.outlook.com > > > > Content-Type: text/plain; charset="iso-8859-1" > > Amplification is the only way. > > Possibly, tyramide amplification will help. > > It will cost you to buy a kit...sure, make your own but....you have to > weigh up pros/cons > > > Sure....you will have to play with variables. > > > > ------------------------------ > > Message: 3 > Date: Fri, 13 Nov 2015 19:42:29 +0000 > From: "Ellenburg, Deborah" > To: Mike Pence > Cc: "histonet at lists.utsouthwestern.edu" >? ? ? ? , >? ? ? ? "histonet-bounces at lists.utsouthwestern.edu" >? ? ? ? > Subject: Re: [Histonet] #ExtMail#? Slide and cassette printers > Message-ID: <66C85B18-BCE2-4F2D-9FF0-7136B605CE72 at bshsi.org> > Content-Type: text/plain; charset="us-ascii" > > We have the ShurMark slide and cassette engraver that interfaces with > CoPath. > > Sent from my iPhone > > > On Nov 12, 2015, at 10:50 AM, Mike Pence via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > > Okay, I am reaching out to the people that I know that has an answer to > everything. What system are you using to print slides and cassettes from in > your lab? Is it intergraded with your LIS and if so what LIS are you using? > I am not looking for a system that prints to labels, I what it to print > directly to the slide or cassette. > > > > Michael S. Pence | Supervisor of Laboratory Services > > Great River Health Systems > > 1221 S. Gear Ave. | West Burlington, IA 52655 > > Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 > > mpence at grhs.net | www.greatrivermedical.org< > http://www.greatrivermedical.org>. > > www.Facebook.com/GreatRiverHealthSystems< > http://www.Facebook.com/GreatRiverHealthSystems> | > www.Twitter/GreatRiverMed > > > > > > Information in this communication, including attachments, is > confidential and intended only for the addressee(s). This communication may > contain privileged, confidential, proprietary or trade secret information > entitled to protection or exemption from disclosure under law. If you are > not an intended recipient, please know that any use, distribution or > copying of this communication, or any action taken based on the information > in this communication, is unauthorized and may be unlawful. If you received > this communication in error, please notify the sender and delete this > communication from your device. > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ________________________________________________________________________________________________________ > The information in this communication is intended to be confidential to > the Individual(s) and/or Entity to whom it is addressed. > It may contain information of a Privileged and/or Confidential nature, > which is subject to Federal and/or State privacy regulations. > In the event that you are not the intended recipient or the agent of the > intended recipient, do not copy or use the information > contained within this communication, or allow it to be read, copied or > utilized in any manner, by any other person(s). Should > this communication be received in error, please notify the sender > immediately either by response e-mail or by phone, > and permanently delete the original e-mail, attachment(s), and any copies. > > ________________________________________________________________________________________________________ > > > > ------------------------------ > > Message: 4 > Date: Sat, 14 Nov 2015 16:39:04 +0000 (UTC) > From: Va Paula Sicurello > To: "histonet at lists.utsouthwestern.edu Histonet" >? ? ? ? > Subject: [Histonet] Histology Lab Survey Questions > Message-ID: >? ? ? ? <970743067.4496622.1447519144079.JavaMail.yahoo at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > Hello Netters, > Thank you for taking my survey. ?I will use your replies for my research > project and I will also let everyone on the list know the results as well. > >From what I've found, the surveys performed in the past asked similar > questions but more at the institution level. ?I would like to get > information at the individual level. ?There are 16 questions. > Please reply to this email offline with your answers. > Thank you for helping me. ?Let's hope I get an A! :-) > Here we go:Questions aboutyou: > > 1.?????Education (highest achieved): > > a.??????High school > > b.?????Associates degree > > c.??????Bachelor?s degree > > d.?????Master?s degree > > e.??????PhD or MD > > 2.?????Certification: > > a.??????None > > b.?????HT > > c.??????HTL > > d.?????Other, please describe > > 3.?????How did you learn histology? > > a.??????On the job > > b.?????Histotechnology school > > 4.?????How many years of experience in histology? > > 5.?????How many blocks can you embed in an hour? > > 6.?????How many of the following can you produce in an 8 > hourday??????????????????????????? ????????????????????(You can calculate > from howmany of each task you can perform in one hour:?20 blocks with one > slide each per hour = 160 blocks and 160 slides) > > a.??????Blocks > > b.?????Slides > > > Questions aboutyour place of work: > > 7.?????How many blocks does your lab embed a day? > > 8.?????How many staff does your lab have? > > a.??????Technical (HT, HTL, etc.) > > b.?????Non-technical (lab assistants, etc.) > > 9.?????How is IHC handled in your lab?? > > a. Separate staff (if so how many?)? > > b. All staff perform > > 10.? Howmany IHC slides does your lab stain per day? > > 11.? Howmany of the following can your lab run? > > a.??????Antibodies (total number available for testing) > > b.?????Special Stains (total number available for testing) > > 12.? Doesyour lab use automation?? Yes or No > > Ifyes, please describe: > > 13.? Whoprepares the slides prior to release to the pathologist? > > 14.? Whofiles the blocks and slides? > > 15.? Whattype of institution do you work at? > > a.??????Hospital based Histology lab > > b.?????Clinic based Histology lab > > c.??????Independent lab (such as a referral lab) > > 16.? BonusQuestion:? Has your family stopped askingwhy you come home with > blue or pink-orange fingers? > > > ????????????????OPTIONAL: Please add your comments, suggestions,or > thoughts. > ?Sincerely, > Paula > Paula SicurelloHistotechnology SpecialistUC San Diego Health > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 144, Issue 14 > ***************************************** > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff at uropartners.com Mon Nov 16 11:49:56 2015 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 16 Nov 2015 17:49:56 +0000 Subject: [Histonet] Hoe everyone had a good weekend,,,non-work related Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B4FC515@COLOEXCH01.uropartners.local> An update on home building http://www.chicagonow.com/downsize-maybe/2015/11/why-a-good-wife-is-better-than-a-pack-of-razor-blades/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From srishan at mail.holyname.org Mon Nov 16 12:31:14 2015 From: srishan at mail.holyname.org (srishan at mail.holyname.org) Date: Mon, 16 Nov 2015 13:31:14 -0500 Subject: [Histonet] slide printer back up Message-ID: Need opinions on this matter. What is the back up plan in your institution, for slide printers and cassette printers? Are you going back to handwriting slides and putting a label or using xylene resistant labels directly on to your slides. Thanks in advance. Nirmala Srishan Histology Supervisor Holy Name Medical Center 718 Teaneck Road Teaneck NJ 07666 Lab: 201 833 3023 Office: 201 541 6328 Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From supervisor at galahistolab.com Mon Nov 16 21:56:36 2015 From: supervisor at galahistolab.com (Rachel) Date: Mon, 16 Nov 2015 21:56:36 -0600 Subject: [Histonet] Seeking Part-time PA Message-ID: Small GI lab is seeking a part time ASCP Certified Pathologist Assistant in Houston, Texas. If any one knows a PA needing in Houston area needing a part time job have them please contact me. Thanks Rachel Pinch 713-783-4252 Sent from my iPhone From robert.h.byrd at gmail.com Tue Nov 17 09:00:30 2015 From: robert.h.byrd at gmail.com (Robert Byrd) Date: Tue, 17 Nov 2015 09:00:30 -0600 Subject: [Histonet] Miles Tissue Tek history Message-ID: Can anyone tell me what years Miles Tissue Tek 4553 cryostats were in manufacture? SN is in the 5000's. General range, maybe half-decade would be good enough for me I've had no luck through Sakura or Bayer Thanks Robert From tbraud at holyredeemer.com Tue Nov 17 13:02:00 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 17 Nov 2015 19:02:00 +0000 Subject: [Histonet] Slide Printer Back-up Message-ID: <48E053DDF6CE074DB6A7414BA05403F804B598@HRHEX02-HOS.holyredeemer.local> Our policy calls for handwriting slides, then applying printed labels at the completion of the stain/coverslip. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Today's Topics: 1. slide printer back up (srishan at mail.holyname.org) Message: 1 Date: Mon, 16 Nov 2015 13:31:14 -0500 From: srishan at mail.holyname.org To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] slide printer back up Need opinions on this matter. What is the back up plan in your institution, for slide printers and cassette printers? Are you going back to handwriting slides and putting a label or using xylene resistant labels directly on to your slides. Thanks in advance. Nirmala Srishan Histology Supervisor Holy Name Medical Center 718 Teaneck Road Teaneck NJ 07666 Lab: 201 833 3023 Office: 201 541 6328 From aeck at dh.org Tue Nov 17 13:14:44 2015 From: aeck at dh.org (Eck, Allison) Date: Tue, 17 Nov 2015 19:14:44 +0000 Subject: [Histonet] processing cycle Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A971A6BBF@DH-MAIL01.dhorg.org> Does anyone have a processing cycle that is good for fatty tissues like breast that they would be willing to share? This will be used on a VIP5. Thank you in advance Allison From mmsamaan at gmail.com Tue Nov 17 22:00:10 2015 From: mmsamaan at gmail.com (Maria Samaan) Date: Tue, 17 Nov 2015 20:00:10 -0800 Subject: [Histonet] Histology technician job, Murrieta, CA Message-ID: We are looking for a qualified applicant, we will have a job open in a few months. Histology Technician The purpose of the Histology Technician is to provide the Pathologist with diagnostic slides to enable them to make a diagnosis. Duties: ? The main duties are: embedding with correct orientation, cutting and staining of histology specimens ? Correctly prepares special stains and solutions ? Performs immunohistochemical stains ? Daily maintenance of laboratory equipment ? Early morning shift, Monday ? Friday at this time, possible Saturday shift assignment or rotation Qualifications: ? 1 year previous experience as a Histology Technician ? High school diploma or equivalent ? ASCP certification is a plus Contact: Susan Holburt Medical Laboratory Services Murrieta, CA 951-834-9020 medlab88 at hotmail.com From AJohnson at aipathology.com Wed Nov 18 06:55:59 2015 From: AJohnson at aipathology.com (Amy Johnson) Date: Wed, 18 Nov 2015 12:55:59 +0000 Subject: [Histonet] Safety question Message-ID: Hello histonetters, I asked this same question about a week ago and got one response hoping to get a little more this time: Chemicals from the manufacturer should be labelled with Pictograms, Signal word, Hazard and Precautionary statements, Product identifier and supplier identification. If you transfer these chemical into another bottle how are you getting all this new info onto the secondary bottle?? Are you creating your own label? Does the manufacturer send extra labels for this purpose? Thanks again for your help, Amy Johnson HTL, ASCP Associates in Pathology Wausau WI, 54401 715-847-2130 From LRaff at uropartners.com Wed Nov 18 10:47:41 2015 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 18 Nov 2015 16:47:41 +0000 Subject: [Histonet] Rain in Chicago--might as well read a blog NOT WORK RELATED Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B50270A@COLOEXCH01.uropartners.local> Hope everyone is having a good week. Today's installment: http://www.chicagonow.com/downsize-maybe/2015/11/what-looked-like-a-step-forward-becomes-a-step-back/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From greg.dobbin at gmail.com Wed Nov 18 12:08:42 2015 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Wed, 18 Nov 2015 14:08:42 -0400 Subject: [Histonet] Safety question Message-ID: Buy yourself a roll of WHMIS labels. Get ones that are sufficiently large to be easily read while still fitting on the side of the secondary container. All necessary info will be captured there once you check the right boxes, etc. Cheers, Greg -- Greg Dobbin, R.T. Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 *Everything in moderation...even moderation itself**!* From tejohnson at genoptix.com Wed Nov 18 13:47:13 2015 From: tejohnson at genoptix.com (Teri Johnson) Date: Wed, 18 Nov 2015 19:47:13 +0000 Subject: [Histonet] Job opening - Genoptix, Carlsbad, CA Message-ID: <06ea31d5d7fe4e6f91a3ff03f515279c@PHUSCB-SP37MB03.genoptix.org> Dear colleagues, We have an opening in our Clinical Trials Testing group, looking for an IHC Specialist/Scientist. Here is the text: Are you looking for a new opportunity with a reputable, fast-growing organization? Do you want to find a place to work that has an exciting global mission, innovative business strategies and makes a positive impact on society? Keep reading. Genoptix Medical Laboratory, a Novartis company, is focused on developing and commercializing evidence-driven diagnostic tests to improve physicians' ability to optimize patient outcomes. We're currently seeking qualified candidates to join our team in the position of: Scientist Position Summary: This position supports performance of novel biomarkers and tissue based platforms for patient testing associated with human clinical trials of cancer drugs. The candidate should be able to manage and execute all aspects of automated IHC assays for multiple antibody targets. Support the organization of the daily workflow, evaluate and review test data, implement quality requirements and prepare associated documentation. The ideal candidate will hold an ASCP HT or ideally an HTL with solid experience in routine surgical pathology. HPC Protocol knowledge is highly desirable. This position may be performing sectioning, paraffin processing, embedding and tissue scrapes for assay development and Clinical Trial Testing (CTT) as a backup to our Histopathology Core Lab as needed. Ensures adherence to company policy on patient and client confidentiality in all circumstances. Essential Duties and Responsibilities: Responsible for writing and reviewing technical documents. Lead internal and external presentations and writing of publications. Manage & execute laboratory research and testing of patient specimens Navigate and troubleshoot complex problems that may adversely affect test performance/results and direct others to resolve problems. Streamline/simplify lab workflow while ensuring compliance. Analyze complex data for trends making recommendations to more senior scientific staff Demonstrated understanding of, and ability to apply principles, concepts, practices and standards associated with analytical assay development/validation and/or sample testing in a clinical laboratory setting. Additional duties as assigned. Education and Qualifications: Degree in Biological Sciences or related scientific discipline with related laboratory experience, including design and execution of related experiments, as noted below: Minimum Education and Qualifications Requires and ASCP certification with either an HT or HTL designation. -5 years' experience working with tissue specimens in a clinical lab setting preferred. Why you should work here? Genoptix offers competitive compensation and benefits programs that include vacation and sick pay, extensive medical insurance coverage, flexible spending accounts, 401 (k) with company match, childcare savings account, commuter benefits and much more! Career development opportunities and educational tuition reimbursement programs. We offer various fitness programs, discounted gym memberships and encourage eco-friendly initiatives like the "Bike to Work Day". Fluid and consistent communications on a quarterly basis from the executive team. Fun and exciting events including "Lab Week" and the annual employee appreciation events. If you or someone you know is looking for a career opportunity with a great company, apply online at www.genoptix.com Genoptix is more than a laboratory - we deliver actionable results to improve quality of life. Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From Richard.Cartun at hhchealth.org Wed Nov 18 15:00:14 2015 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Wed, 18 Nov 2015 21:00:14 +0000 Subject: [Histonet] IgG4 Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B08A8F1@HHCEXCHMB03.hhcsystem.org> Hi everyone. Which antibody (clone) are you using for the immunohistochemical detection of IgG4 in FFPE tissue? I just found out that the clone that we were using is no longer available. Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From JRobinson at pathology-associates.com Wed Nov 18 16:43:08 2015 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Wed, 18 Nov 2015 22:43:08 +0000 Subject: [Histonet] IgG4 In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E6B08A8F1@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E6B08A8F1@HHCEXCHMB03.hhcsystem.org> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16DA1B67@PAEXCH1.PathologyAssociates.local> Hi Richard- I have been using the CellMarque IgG4 (MRQ-44 clone), mouse monoclonal on the Leica Bond with success. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, November 18, 2015 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IgG4 Hi everyone. Which antibody (clone) are you using for the immunohistochemical detection of IgG4 in FFPE tissue? I just found out that the clone that we were using is no longer available. Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From badams at acadianagastro.com Wed Nov 18 17:01:06 2015 From: badams at acadianagastro.com (Brent Adams) Date: Wed, 18 Nov 2015 23:01:06 +0000 Subject: [Histonet] Histonet Digest, Vol 144, Issue 17 topic 1 slide printer back up In-Reply-To: References: Message-ID: Our facility policy also calls for handwritten before staining and printed labels after staining. Brent Adams ? BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Wednesday, November 18, 2015 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 144, Issue 17 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Slide Printer Back-up (Terri Braud) 2. processing cycle (Eck, Allison) 3. Histology technician job, Murrieta, CA (Maria Samaan) 4. Safety question (Amy Johnson) 5. Rain in Chicago--might as well read a blog NOT WORK RELATED (Lester Raff MD) ---------------------------------------------------------------------- Message: 1 Date: Tue, 17 Nov 2015 19:02:00 +0000 From: "Terri Braud" To: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] Slide Printer Back-up Message-ID: <48E053DDF6CE074DB6A7414BA05403F804B598 at HRHEX02-HOS.holyredeemer.local> Content-Type: text/plain; charset="us-ascii" Our policy calls for handwriting slides, then applying printed labels at the completion of the stain/coverslip. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Today's Topics: 1. slide printer back up (srishan at mail.holyname.org) Message: 1 Date: Mon, 16 Nov 2015 13:31:14 -0500 From: srishan at mail.holyname.org To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] slide printer back up Need opinions on this matter. What is the back up plan in your institution, for slide printers and cassette printers? Are you going back to handwriting slides and putting a label or using xylene resistant labels directly on to your slides. Thanks in advance. Nirmala Srishan Histology Supervisor Holy Name Medical Center 718 Teaneck Road Teaneck NJ 07666 Lab: 201 833 3023 Office: 201 541 6328 ------------------------------ Message: 2 Date: Tue, 17 Nov 2015 19:14:44 +0000 From: "Eck, Allison" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] processing cycle Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A971A6BBF at DH-MAIL01.dhorg.org> Content-Type: text/plain; charset="us-ascii" Does anyone have a processing cycle that is good for fatty tissues like breast that they would be willing to share? This will be used on a VIP5. Thank you in advance Allison ------------------------------ Message: 3 Date: Tue, 17 Nov 2015 20:00:10 -0800 From: Maria Samaan To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Histology technician job, Murrieta, CA Message-ID: Content-Type: text/plain; charset=UTF-8 We are looking for a qualified applicant, we will have a job open in a few months. Histology Technician The purpose of the Histology Technician is to provide the Pathologist with diagnostic slides to enable them to make a diagnosis. Duties: ? The main duties are: embedding with correct orientation, cutting and staining of histology specimens ? Correctly prepares special stains and solutions ? Performs immunohistochemical stains ? Daily maintenance of laboratory equipment ? Early morning shift, Monday ? Friday at this time, possible Saturday shift assignment or rotation Qualifications: ? 1 year previous experience as a Histology Technician ? High school diploma or equivalent ? ASCP certification is a plus Contact: Susan Holburt Medical Laboratory Services Murrieta, CA 951-834-9020 medlab88 at hotmail.com ------------------------------ Message: 4 Date: Wed, 18 Nov 2015 12:55:59 +0000 From: Amy Johnson To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Safety question Message-ID: Content-Type: text/plain; charset="us-ascii" Hello histonetters, I asked this same question about a week ago and got one response hoping to get a little more this time: Chemicals from the manufacturer should be labelled with Pictograms, Signal word, Hazard and Precautionary statements, Product identifier and supplier identification. If you transfer these chemical into another bottle how are you getting all this new info onto the secondary bottle?? Are you creating your own label? Does the manufacturer send extra labels for this purpose? Thanks again for your help, Amy Johnson HTL, ASCP Associates in Pathology Wausau WI, 54401 715-847-2130 ------------------------------ Message: 5 Date: Wed, 18 Nov 2015 16:47:41 +0000 From: Lester Raff MD To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Rain in Chicago--might as well read a blog NOT WORK RELATED Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B50270A at COLOEXCH01.uropartners.local> Content-Type: text/plain; charset="us-ascii" Hope everyone is having a good week. Today's installment: http://www.chicagonow.com/downsize-maybe/2015/11/what-looked-like-a-step-forward-becomes-a-step-back/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 144, Issue 17 ***************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. From badams at acadianagastro.com Wed Nov 18 17:17:20 2015 From: badams at acadianagastro.com (Brent Adams) Date: Wed, 18 Nov 2015 23:17:20 +0000 Subject: [Histonet] Histonet Digest, Vol 144, Issue 17 Topic 4 In-Reply-To: References: Message-ID: Amy, >From what I have gathered From the OSHA site, The Global Harmonized System (GHS) only requires Manufactures and Importers to use the Pictograms. The labels NFP and HMIS do not appear to need these according to the web site. This change is mostly for the US Chemical manufactures and Importers to more Universal with the rest of the world. We should use make sure that we have the current SDS for each Reagent you use. sorry I cant be more help, we are also struggling with the changes. I am certain that in a couple years the inspectors will make a note of mentioning how they want us to meet the guidelines. Brent Adams ? BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Wednesday, November 18, 2015 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 144, Issue 17 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Slide Printer Back-up (Terri Braud) 2. processing cycle (Eck, Allison) 3. Histology technician job, Murrieta, CA (Maria Samaan) 4. Safety question (Amy Johnson) 5. Rain in Chicago--might as well read a blog NOT WORK RELATED (Lester Raff MD) ---------------------------------------------------------------------- Message: 1 Date: Tue, 17 Nov 2015 19:02:00 +0000 From: "Terri Braud" To: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] Slide Printer Back-up Message-ID: <48E053DDF6CE074DB6A7414BA05403F804B598 at HRHEX02-HOS.holyredeemer.local> Content-Type: text/plain; charset="us-ascii" Our policy calls for handwriting slides, then applying printed labels at the completion of the stain/coverslip. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Today's Topics: 1. slide printer back up (srishan at mail.holyname.org) Message: 1 Date: Mon, 16 Nov 2015 13:31:14 -0500 From: srishan at mail.holyname.org To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] slide printer back up Need opinions on this matter. What is the back up plan in your institution, for slide printers and cassette printers? Are you going back to handwriting slides and putting a label or using xylene resistant labels directly on to your slides. Thanks in advance. Nirmala Srishan Histology Supervisor Holy Name Medical Center 718 Teaneck Road Teaneck NJ 07666 Lab: 201 833 3023 Office: 201 541 6328 ------------------------------ Message: 2 Date: Tue, 17 Nov 2015 19:14:44 +0000 From: "Eck, Allison" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] processing cycle Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A971A6BBF at DH-MAIL01.dhorg.org> Content-Type: text/plain; charset="us-ascii" Does anyone have a processing cycle that is good for fatty tissues like breast that they would be willing to share? This will be used on a VIP5. Thank you in advance Allison ------------------------------ Message: 3 Date: Tue, 17 Nov 2015 20:00:10 -0800 From: Maria Samaan To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Histology technician job, Murrieta, CA Message-ID: Content-Type: text/plain; charset=UTF-8 We are looking for a qualified applicant, we will have a job open in a few months. Histology Technician The purpose of the Histology Technician is to provide the Pathologist with diagnostic slides to enable them to make a diagnosis. Duties: ? The main duties are: embedding with correct orientation, cutting and staining of histology specimens ? Correctly prepares special stains and solutions ? Performs immunohistochemical stains ? Daily maintenance of laboratory equipment ? Early morning shift, Monday ? Friday at this time, possible Saturday shift assignment or rotation Qualifications: ? 1 year previous experience as a Histology Technician ? High school diploma or equivalent ? ASCP certification is a plus Contact: Susan Holburt Medical Laboratory Services Murrieta, CA 951-834-9020 medlab88 at hotmail.com ------------------------------ Message: 4 Date: Wed, 18 Nov 2015 12:55:59 +0000 From: Amy Johnson To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Safety question Message-ID: Content-Type: text/plain; charset="us-ascii" Hello histonetters, I asked this same question about a week ago and got one response hoping to get a little more this time: Chemicals from the manufacturer should be labelled with Pictograms, Signal word, Hazard and Precautionary statements, Product identifier and supplier identification. If you transfer these chemical into another bottle how are you getting all this new info onto the secondary bottle?? Are you creating your own label? Does the manufacturer send extra labels for this purpose? Thanks again for your help, Amy Johnson HTL, ASCP Associates in Pathology Wausau WI, 54401 715-847-2130 ------------------------------ Message: 5 Date: Wed, 18 Nov 2015 16:47:41 +0000 From: Lester Raff MD To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Rain in Chicago--might as well read a blog NOT WORK RELATED Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B50270A at COLOEXCH01.uropartners.local> Content-Type: text/plain; charset="us-ascii" Hope everyone is having a good week. Today's installment: http://www.chicagonow.com/downsize-maybe/2015/11/what-looked-like-a-step-forward-becomes-a-step-back/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 144, Issue 17 ***************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. From a.prior at tissueregenix.com Thu Nov 19 02:30:10 2015 From: a.prior at tissueregenix.com (Andrew Prior) Date: Thu, 19 Nov 2015 08:30:10 +0000 Subject: [Histonet] Safety Question Message-ID: Hi Amy, We try to keep everything in its original bottles, but create our own labels if we do decant into a new bottle. We include Reagent name and conc., expiry date and lot no., and hazards. We also use hazard pictogram labels - available on rolls from most major suppliers. Alongside the labels we have the Safety Data Sheets in the lab and there are COSHH assessments for all chemicals which users have to read and sign before starting work. If you use secondary bottles frequently, then it's probably worth spending 5 min setting up label templates so that everyone in your lab knows what info is required. Hope that helps Andrew Andrew Prior Histologist Tissue Regenix Ltd ------------------------------ Message: 4 Date: Wed, 18 Nov 2015 12:55:59 +0000 From: Amy Johnson To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Safety question Message-ID: Content-Type: text/plain; charset="us-ascii" Hello histonetters, I asked this same question about a week ago and got one response hoping to get a little more this time: Chemicals from the manufacturer should be labelled with Pictograms, Signal word, Hazard and Precautionary statements, Product identifier and supplier identification. If you transfer these chemical into another bottle how are you getting all this new info onto the secondary bottle?? Are you creating your own label? Does the manufacturer send extra labels for this purpose? Thanks again for your help, Amy Johnson HTL, ASCP Associates in Pathology Wausau WI, 54401 715-847-2130 ******* From eroy at illinois.edu Thu Nov 19 09:22:04 2015 From: eroy at illinois.edu (Roy, Edward J) Date: Thu, 19 Nov 2015 15:22:04 +0000 Subject: [Histonet] Bodipy stains Message-ID: Does anyone have experience with Bodipy 493/503 or Bodipy 581/591 lipid stains with histological sections? I?ve seen one paper using muscle sections with Bodipy 493/503, but wondered what processing steps would mess it up. Specifically, will it still work with formaldehyde fixed sections, processed through 30% sucrose and cryosectioned? Or just snap frozen and cryosectioned? I presume if sections go through any ethanol the lipids would get extracted. Thanks for any advice. Ed Roy Edward J. Roy, PhD Professor Emeritus, Department of Pathology University of Illinois at Urbana-Champaign Urbana, IL 61801 217 333-3375 From HornHV at archildrens.org Thu Nov 19 14:39:30 2015 From: HornHV at archildrens.org (Horn, Hazel V) Date: Thu, 19 Nov 2015 20:39:30 +0000 Subject: [Histonet] cpt codes Message-ID: Histonetters, The cpt code for a colostomy stoma is 88304. Would you consider and ileostomy the same cpt code? Thanks. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org From vapatpxs at yahoo.com Thu Nov 19 22:33:24 2015 From: vapatpxs at yahoo.com (Va Paula Sicurello) Date: Fri, 20 Nov 2015 04:33:24 +0000 (UTC) Subject: [Histonet] Histology Questions part 2 References: <221177247.7366245.1447994004220.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <221177247.7366245.1447994004220.JavaMail.yahoo@mail.yahoo.com> Good Evening Netters, I've created a short survey using Survey Monkey. ?Please take my survey and help me gather information I can use in my class and to help update the productivity standards and staffing benchmarks for our Histology labs. https://www.surveymonkey.com/r/8HFSY3V Thank you and thanks to those who took my first survey. ?The results look interesting. Sincerely, Paula Paula SicurelloHistotechnology SpecialistUC San Diego Health From gu.lang at gmx.at Fri Nov 20 01:59:14 2015 From: gu.lang at gmx.at (Gudrun Lang) Date: Fri, 20 Nov 2015 08:59:14 +0100 Subject: [Histonet] processing cycle In-Reply-To: <4ED8C96A8F20FC4F883A92E2A0A0D64A971A6BBF@DH-MAIL01.dhorg.org> References: <4ED8C96A8F20FC4F883A92E2A0A0D64A971A6BBF@DH-MAIL01.dhorg.org> Message-ID: <005001d12369$5a8c56e0$0fa504a0$@gmx.at> Hi Allison, we doubled the times of the regular processing protocol beginning with longer absolute ethanol, intermedium and paraffin. Our regular protocol takes 13 hours and the fatty-protocol takes 17 hours. We start it at about 2 pm with endtime at 8 am. So breast tissue is mostly embedded at the end of the bulk of cassettes. This improved cutting really in a great manner. The problem occurs, that fixation time is rather short, when grossing of breast is done just shortly before processing. So we want our pathologists to gross the breast from the day before rather early in the morning. Gudrun -----Urspr?ngliche Nachricht----- Von: Eck, Allison via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Dienstag, 17. November 2015 20:15 An: 'histonet at lists.utsouthwestern.edu' Betreff: [Histonet] processing cycle Does anyone have a processing cycle that is good for fatty tissues like breast that they would be willing to share? This will be used on a VIP5. Thank you in advance Allison _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From blayjorge at gmail.com Fri Nov 20 07:02:49 2015 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Fri, 20 Nov 2015 08:02:49 -0500 Subject: [Histonet] looking for (ideally) recent and comprehensive papers containing cross sections (light microscopy and/or transmission electron microscopy) of insect wings Message-ID: Hello Histonet-Listers: I am looking for (ideally) recent and comprehensive papers containing cross sections (light microscopy and/or transmission electron microscopy) of insect wings. If you know of such refs, bibliographic info and/or a pdf will be appreciated (please, send them to blayjorge at gmail.com , not to the list). Apologies for potential duplicate emails. Gratefully, Jorge From MICHELLE.LAMPHERE at childrens.com Fri Nov 20 08:11:24 2015 From: MICHELLE.LAMPHERE at childrens.com (Michelle Lamphere) Date: Fri, 20 Nov 2015 14:11:24 +0000 Subject: [Histonet] EBSciences microwave tissue processor Message-ID: Good morning Has anybody ever experienced a problem with their EBSciences microwave where the temperature probe failed during a cycle and the reagent continued to heat? For anybody who uses the microwaves, have you experienced any other mechanical issues that could affect your microwave processing? Also, are there microwave processors on the market that have safety measures in place to prevent this sort of error? Michelle Lamphere, HT(ASCP) Lead Tech, Histology Department of Anatomic Pathology 1935 Medical District Dr. Dallas, TX 75235 214.456.2318 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at privacy at childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at privacy at childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From kp5124 at outlook.com Fri Nov 20 09:24:00 2015 From: kp5124 at outlook.com (Karen Pfaff) Date: Fri, 20 Nov 2015 15:24:00 +0000 Subject: [Histonet] =?utf-8?q?Mohs_and_what_is_considered_grossing=3F?= Message-ID: In my lab I am checking my CAP checklist. I just took over the Supervisors job after she retired. In looking at our checklist it mentions that Mohs testing is a High complexity test which requires qualifications. Part of the checklist states ?Grossing specimens -nonpathologist.? In research to see if Mohs actually considers Grossing as part of there protocol. Surgeon places tissue on a piece of guaze with proper orientation. Designates where hatches or scores are located (12 o?clock, 3 o?clock,etc..) Hands it over to tech. The tech then draws map of specimen, bisects it usually across the scores, depending on size of specimen could be more pieced if larger. The tech then inks specimen, relaxes tissue so specimen will lay down properly. Gives an accession number to it then embed tissue in OCT and continues to cut tissue and place on slide. The slide is then stained, coverslipped and handed over to Mohs surgeon. Just wondering if this part of manipulating the tissue is considered grossing. Thank you for any information you may have. Karen Sent from Windows Mail Sent from Windows Mail From LRaff at uropartners.com Fri Nov 20 09:44:56 2015 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 20 Nov 2015 15:44:56 +0000 Subject: [Histonet] Non-work related blog postbut not exactly Friday fun Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B50A682@COLOEXCH01.uropartners.local> I get a bit serious with this blog Comments invited http://www.chicagonow.com/downsize-maybe/2015/11/getting-serious-about-a-bad-medicare-recommendation/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From nguy0515 at gmail.com Fri Nov 20 16:40:47 2015 From: nguy0515 at gmail.com (Trini Nguyen) Date: Fri, 20 Nov 2015 14:40:47 -0800 Subject: [Histonet] Job Opening- Dana Point, CA Message-ID: Prominent, upscale, three physician dermatology practice seeking a full time (Mon-Fri) histotechnician with Mohs experience for Dermatology clinic located in Dana Point, CA. The technician will assist in Mohs tissue processing once a week and paraffin encompassed tasks on all other days. *Responsibilities include:* ? Mohs frozen section preparation Mapping, inking, embedding, sectioning, staining and manual coverslipping ? Paraffin section preparation Grossing, processing, embedding, sectioning, staining and manual coverslipping ? Maintain Mohs and biopsy logbooks Record keeping for blocks and slides sent out for consultation or special stains. Filing slides and blocks as necessary. ? Maintain equipment and comply with CLIA regulations Perform maintenance on equipment as needed and troubleshoot when needed. Record on logs all maintenance and daily usage. *Experience/Qualifications:* ? At least one (1) year of experience in a technical role in a histology lab and Mohs surgery ? Excellent organization skills ? Ability to perform tasks and meet deadlines unsupervised *Preferred but not required:* ? HT certification ? Prior experience in dermatology *Generous benefits package includes: Medical and vision insurance and 401K, excellent work environment* From bcooper at chla.usc.edu Fri Nov 20 19:22:36 2015 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Sat, 21 Nov 2015 01:22:36 +0000 Subject: [Histonet] Document Control Systems Message-ID: Happy Friday Histonetters! For any of you out there using Title 21, Medialab or iPassport for document control (or anything else for that matter), I would love to hear your feedback on what it's like to use the systems. Pros/cons/responsiveness of the companies to your concerns-anything at all! Your feedback would be greatly appreciated! You can email me offline if you prefer. We've seen demos from all three vendors, but would absolutely love to hear from people who actually use these systems. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper at chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From thisisann at aol.com Mon Nov 23 12:16:13 2015 From: thisisann at aol.com (Ann Specian) Date: Mon, 23 Nov 2015 13:16:13 -0500 Subject: [Histonet] guidelines for using digital images as a primary diagnostic tool Message-ID: <151358f78fc-680f-e53c@webprd-a70.mail.aol.com> Does anyone have any information in regard to using digitalimages as the primary diagnostic tool? Thanks, ann From jpiche at wtbyhosp.org Mon Nov 23 14:18:49 2015 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Mon, 23 Nov 2015 20:18:49 +0000 Subject: [Histonet] Automatic coverslippers Message-ID: <631955447A364B45B9458D2905635110F0FBC978@WIN08-MBX-02.wtbyhosp.org> Hi Everyone, Just looking for opinions here....What automatic cover slipper do techs like best? And why? Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From camgomes10 at gmail.com Tue Nov 24 04:50:40 2015 From: camgomes10 at gmail.com (Camila Gomes) Date: Tue, 24 Nov 2015 10:50:40 +0000 Subject: [Histonet] Masson's Trichrome Stain Message-ID: Dear all, I did a Masson's Trichrome Stain in frozen liver samples with cancer and it turns quite well to differentiate the collagen in the tumour from the normal liver but I would also like to see better the nuclei detail and have a less intense pink. Here is the protocol that I am using with the Sigma kit: (Fixation with 4%PFA for 10 minutes) 1- Bouin's Solution at room temperature over-night; 2- Wash in tap water until remove yellow color; 3- Weigert's Hematoxilin for 5 min; 4- Was in tap water for 5 min; 5- Rinse in deionized water; 6- Biebrich Scarlet-Acid Fucshin for 5 minutes; 7- Rinse in deionized water; 8- Phosphomolybdic Acid for 5 minutes; 9- Aniline blue solution for 5 minutes; 10-Acetic acid 1% 1'30''; 11-Rinse in water, dehydrate, clear and mount. All the solution are made at the moment of the staining. I have experience with the staining but only in paraffin embedded sections and I would like to know if there is any step to be careful or change with frozen sections. Anyone can help me please? Thank you, Camila From LRaff at uropartners.com Tue Nov 24 09:22:26 2015 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 24 Nov 2015 15:22:26 +0000 Subject: [Histonet] Not work related Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B520BB9@COLOEXCH01.uropartners.local> Just for fun today www.chicagonow.com/downsize-maybe/2015/11/we-want-to-be-on-hgtv-too/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From jvickroy at SpringfieldClinic.com Tue Nov 24 11:04:38 2015 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Tue, 24 Nov 2015 17:04:38 +0000 Subject: [Histonet] Rapid tissue Programs Message-ID: <9B1A1501A800064397369BD8072E6BCA064C059C@E2K10DB.springfieldclinic.com> Our latest CAP survey was returned today and although there are no major issues one possible improvement area is evident. On all of the biopsies the area of "fixation/processing" was not rated as excellent. The suggested possible reasons were: fixation incomplete, nuclear bubbling artifact, tissue poorly processed. The survey also said that many of the samples sent in throughout the country had similar issues in the fixation/processing area most likely because of the rapid turnaround times and shortened processing times. I am trying to be proactive here and see if we can adjust some times to improve the processing quality even though we have not had any complaints from the pathologists. Of course we all know that other artifacts caused prior to the specimen arriving in the lab can also have an effect on the quality of the H&E slides. Our fixation times should not be a factor so I have to conclude that maybe the rest of our processing times need to be adjusted. Another factor that we have is that we use blue sponges for almost all of our tissues. Our largest number of specimens are GI biopsies. If possible can anyone share with me their rapid processing schedules or simply the approximate times they have for each dehydration or clearing step. We do run a larger overnight tissue run on any biopsy or tissue that we feel is too large for the "rapid run". I am hesitant to run the biopsies routinely on the longer programs becase of over dehydration, etc. even though we do use an alcohol blend. Any suggestions or similar experiences please share. Again our pathologists say everything looks great so I don't want to change much. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From wdesalvo.cac at outlook.com Tue Nov 24 12:17:42 2015 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Tue, 24 Nov 2015 11:17:42 -0700 Subject: [Histonet] Rapid tissue Programs Message-ID: First suggestion is to remove the sponge. There will be carry over on biopsy/rapid tissue processor schedules. The sponges do require longer times to drain and exchange liquids. Your fixation times must be >6 hours. Make sure you are not applying too much heat to processing. Small biopsies are delicate and exchange of fluids/paraffin should not need physical elements to assist. Additionally, do not over dehydrate through alcohols. You processor schedule will need to be validated, if you make time/ pressure/heat or reagent change. Good luck in the problem solving process. Sent from my Windows Phone ________________________________ From: Vickroy, James via Histonet Sent: ?11/?24/?2015 10:27 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Rapid tissue Programs Our latest CAP survey was returned today and although there are no major issues one possible improvement area is evident. On all of the biopsies the area of "fixation/processing" was not rated as excellent. The suggested possible reasons were: fixation incomplete, nuclear bubbling artifact, tissue poorly processed. The survey also said that many of the samples sent in throughout the country had similar issues in the fixation/processing area most likely because of the rapid turnaround times and shortened processing times. I am trying to be proactive here and see if we can adjust some times to improve the processing quality even though we have not had any complaints from the pathologists. Of course we all know that other artifacts caused prior to the specimen arriving in the lab can also have an effect on the quality of the H&E slides. Our fixation times should not be a factor so I have to conclude that maybe the rest of our processing times need to be adjusted. Another factor that we have is that we use blue sponges for almost all of our tissues. Our largest number of specimens are GI biopsies. If possible can anyone share with me their rapid processing schedules or simply the approximate times they have for each dehydration or clearing step. We do run a larger overnight tissue run on any biopsy or tissue that we feel is too large for the "rapid run". I am hesitant to run the biopsies routinely on the longer programs becase of over dehydration, etc. even though we do use an alcohol blend. Any suggestions or similar experiences please share. Again our pathologists say everything looks great so I don't want to change much. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Tue Nov 24 12:20:09 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 24 Nov 2015 18:20:09 +0000 (UTC) Subject: [Histonet] Rapid tissue Programs In-Reply-To: <9B1A1501A800064397369BD8072E6BCA064C059C@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA064C059C@E2K10DB.springfieldclinic.com> Message-ID: <1545132733.8117568.1448389209404.JavaMail.yahoo@mail.yahoo.com> You point out to several issues that I would like to address:1- "nuclear bubbling" has nothing to do with processing. This is a post-sectioning artifact appearing when there is water underneath the section when it is set to dry before staining. Just make sure you shake the slide with the section and set it to drain vertically before placing them into the oven to dry.2- sponges can be a source of problems influencing dehydration and even causing "marks" on the tissue that can later produce an artifact. Try to avoid sponges and use tissue paper to wrap the biopsies instead.3- fear not placing small biopsies in the overnight "long process". If the protocol is well balances you will have no problems. In all the years I oversaw tissue processing ine 2 labs I supervised (one with an excess of 35,000 cases/years and the other close to 200,000 cases/year) I had only one protocol for everything, except for fatty tissues, breast and brain.The time in every reagent is not really the issue but the gradient you use. Abrupt changes (like starting with 90% alcohols) or not having "mixed steps" (1:1 ratios) between last alcohol and clearing agent or between clearing agent and melted paraffin are the real causes of the so called "harsh" processing. If you go to http://www.histosearch.com/rene/html?you will find my "standard" protocols.Ren? On Tuesday, November 24, 2015 12:24 PM, "Vickroy, James via Histonet" wrote: Our latest CAP survey was returned today and although there are no major issues one possible improvement area is evident.? On all of the biopsies the area of "fixation/processing" was not rated as excellent.? The suggested possible reasons were:? fixation incomplete, nuclear bubbling artifact, tissue poorly processed.? The survey also said that many of the samples sent in throughout the country had similar? issues in the fixation/processing area most likely because of the rapid turnaround times and shortened processing times.? I am trying to be proactive here and see if we can adjust some times to improve the processing quality even though we have not had any complaints from the pathologists.? Of course we all know that other artifacts caused prior to the specimen arriving in the lab can also have an effect on the quality of the H&E slides.? Our fixation times should not be a factor so I have to conclude that maybe the rest of our processing times need to be adjusted.? Another factor that we have is that we use blue sponges for almost all of our tissues.? Our largest number of specimens are GI biopsies.? ? If possible can anyone share with me their rapid processing schedules or simply the approximate times they have for each dehydration or clearing step. We do run a larger overnight tissue run on any biopsy or tissue that we feel is too large for the "rapid run".? ? I am hesitant to run the biopsies routinely on the longer programs becase of over dehydration, etc. even though we do use an alcohol blend. Any suggestions or similar experiences please share.? ? Again our pathologists say everything looks great so I don't want to change much. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois? 62703 Office:? 217-528-7541, Ext. 15121 Email:? jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ryan.Roy at va.gov Tue Nov 24 12:37:30 2015 From: Ryan.Roy at va.gov (Roy, Ryan) Date: Tue, 24 Nov 2015 13:37:30 -0500 Subject: [Histonet] Histology position Message-ID: <15F883394EAB744E99E1C7E1B9873049018C1ECD1210@R04BYNMSGB1.r04.med.va.gov> Hello Histonetters, The Manchester VA Medical Center in Manchester NH currently has a critical need for a qualified Histologist. It's an excellent position with great benefits. If you are interested please review the link below. Any further questions can be directed to contact Kevin Hall at (603)624-4366 ext. 6638. Feel free to share the link. https://www.usajobs.gov/GetJob/ViewDetails/422133100 This is not a third party posting. Ryan Roy HTL (ASCP) Histology Lab Manchester VA 718 Symth Rd Manchester NH 03104 (603) 624-4366 ex 6640 Disclosure: The content of this email does not reflect the policies, views or opinions of the VA. From SteveM at mcclainlab.com Tue Nov 24 14:07:21 2015 From: SteveM at mcclainlab.com (Steve McClain) Date: Tue, 24 Nov 2015 20:07:21 +0000 Subject: [Histonet] Histonet Digest, Vol 144, Issue 21: Rapid tissue Programs In-Reply-To: References: Message-ID: Since processing is not the issue, first standardize fixation, eg 4-6 hours before processing or in the first reagent on processor. Change that first formalin every 200 blocks or so. When well-fixed, small tissues will tolerate rapid processing. Finally, if your pathologists can read the slides and your immunostains work, then only make small changes. And ignore the CAP. Steve A. McClain, MD From steven.weston at utas.edu.au Tue Nov 24 16:05:14 2015 From: steven.weston at utas.edu.au (Steven Weston) Date: Tue, 24 Nov 2015 22:05:14 +0000 Subject: [Histonet] re automatic cover slippers Message-ID: We use the Dako cover slipper and provided the moving arm is kept free from dried mounting medium we have found this to be a terrific machine. It also has a really small footprint and so fits nicely into our fume hood. Can do about 400 slides an hour and produces very few bubbles. Steve Weston Breathe-Well CRE Lab Manager 0408990859 University of Tasmania Electronic Communications Policy (December, 2014). This email is confidential, and is for the intended recipient only. Access, disclosure, copying, distribution, or reliance on any of it by anyone outside the intended recipient organisation is prohibited and may be a criminal offence. Please delete if obtained in error and email confirmation to the sender. The views expressed in this email are not necessarily the views of the University of Tasmania, unless clearly intended otherwise. From dgoodwin at princetonhcs.org Wed Nov 25 08:39:43 2015 From: dgoodwin at princetonhcs.org (goodwin, diana) Date: Wed, 25 Nov 2015 09:39:43 -0500 Subject: [Histonet] Are gloves required when cutting FFPE blocks? Message-ID: <667F0DDD9B242E40B448C2CDED9409970A6F8642EE@exch-ms-02.PHCS.ORG> Can anyone provide a reference as to whether or not gloves are required when cutting routine FFPE blocks? Diana Goodwin UMCPP Anatomic Pathology Supervisor Office 609-853-6808 Histology 609-853-16860 This e-mail transmission and any documents attached hereto contain information from Princeton HealthCare System which is confidential and/or legally privileged. This information is intended only for the use of the individual or entity to which it is addressed, and only for the purpose for which this transmission was made. Any other use, disclosure, copying, distribution, re-transmission, or the taking of any action in reliance upon the contents of this information is strictly prohibited. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, re-transmission or the taking of any action in reliance upon the contents of this information is strictly prohibited, and that this e-mail transmission and accompanying documents should be returned to Princeton HealthCare System immediately. If you have received this e-mail transmission in error, please notify the sender immediately and destroy all copies of the information contained in this transmission. The above signature block is intended for identification purposes only and does not constitute affirmation that a binding contract has been created via this e-mail communication unless expressly so stated within the body of this e-mail transmission. From rjbuesa at yahoo.com Wed Nov 25 09:04:19 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 25 Nov 2015 15:04:19 +0000 (UTC) Subject: [Histonet] Are gloves required when cutting FFPE blocks? In-Reply-To: <667F0DDD9B242E40B448C2CDED9409970A6F8642EE@exch-ms-02.PHCS.ORG> References: <667F0DDD9B242E40B448C2CDED9409970A6F8642EE@exch-ms-02.PHCS.ORG> Message-ID: <2090026182.6706289.1448463859775.JavaMail.yahoo@mail.yahoo.com> NOT AT ALL, unless you are sectioning a suspected or known Creutzfeldt-Jakob's disease case. Check CAP regulations.Ren? On Wednesday, November 25, 2015 9:56 AM, "goodwin, diana via Histonet" wrote: Can anyone provide a reference as to whether or not gloves are required when cutting routine FFPE blocks? Diana Goodwin UMCPP Anatomic Pathology Supervisor Office 609-853-6808 Histology 609-853-16860 This e-mail transmission and any documents attached hereto contain information from Princeton HealthCare System which is confidential and/or legally privileged.? This information is intended only for the use of the individual or entity to which it is addressed, and only for the purpose for which this transmission was made.? Any other use, disclosure, copying, distribution, re-transmission, or the taking of any action in reliance upon the contents of this information is strictly prohibited.? If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, re-transmission or the taking of any action in reliance upon the contents of this information is strictly prohibited, and that this e-mail transmission and accompanying documents should be returned to Princeton HealthCare System immediately.? If you have received this e-mail transmission in error, please notify the sender immediately and destroy all copies of the information contained in this transmission.? The above signature block is intended for identification purposes only and does not constitute affirmation that a binding contract has been created via this e-mail communication unless expressly so stated within the body of this e-mail transmission. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dgoodwin at princetonhcs.org Wed Nov 25 09:08:53 2015 From: dgoodwin at princetonhcs.org (goodwin, diana) Date: Wed, 25 Nov 2015 10:08:53 -0500 Subject: [Histonet] Are gloves required when cutting FFPE blocks? In-Reply-To: <2090026182.6706289.1448463859775.JavaMail.yahoo@mail.yahoo.com> References: <667F0DDD9B242E40B448C2CDED9409970A6F8642EE@exch-ms-02.PHCS.ORG> <2090026182.6706289.1448463859775.JavaMail.yahoo@mail.yahoo.com> Message-ID: <667F0DDD9B242E40B448C2CDED9409970A6F8642F0@exch-ms-02.PHCS.ORG> We were cited for this by CAP. I need a specific reference to rebut or comply. Nothing specific to routine paraffin microtomy in CAP checklists. dg From: Rene J Buesa [mailto:rjbuesa at yahoo.com] Sent: Wednesday, November 25, 2015 10:04 AM To: goodwin, diana; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Are gloves required when cutting FFPE blocks? NOT AT ALL, unless you are sectioning a suspected or known Creutzfeldt-Jakob's disease case. Check CAP regulations. Ren? On Wednesday, November 25, 2015 9:56 AM, "goodwin, diana via Histonet" > wrote: Can anyone provide a reference as to whether or not gloves are required when cutting routine FFPE blocks? Diana Goodwin UMCPP Anatomic Pathology Supervisor Office 609-853-6808 Histology 609-853-16860 This e-mail transmission and any documents attached hereto contain information from Princeton HealthCare System which is confidential and/or legally privileged. This information is intended only for the use of the individual or entity to which it is addressed, and only for the purpose for which this transmission was made. Any other use, disclosure, copying, distribution, re-transmission, or the taking of any action in reliance upon the contents of this information is strictly prohibited. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, re-transmission or the taking of any action in reliance upon the contents of this information is strictly prohibited, and that this e-mail transmission and accompanying documents should be returned to Princeton HealthCare System immediately. If you have received this e-mail transmission in error, please notify the sender immediately and destroy all copies of the information contained in this transmission. The above signature block is intended for identification purposes only and does not constitute affirmation that a binding contract has been created via this e-mail communication unless expressly so stated within the body of this e-mail transmission. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail transmission and any documents attached hereto contain information from Princeton HealthCare System which is confidential and/or legally privileged. This information is intended only for the use of the individual or entity to which it is addressed, and only for the purpose for which this transmission was made. Any other use, disclosure, copying, distribution, re-transmission, or the taking of any action in reliance upon the contents of this information is strictly prohibited. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, re-transmission or the taking of any action in reliance upon the contents of this information is strictly prohibited, and that this e-mail transmission and accompanying documents should be returned to Princeton HealthCare System immediately. If you have received this e-mail transmission in error, please notify the sender immediately and destroy all copies of the information contained in this transmission. The above signature block is intended for identification purposes only and does not constitute affirmation that a binding contract has been created via this e-mail communication unless expressly so stated within the body of this e-mail transmission. From paula at excaliburpathology.com Wed Nov 25 09:37:12 2015 From: paula at excaliburpathology.com (paula at excaliburpathology.com) Date: Wed, 25 Nov 2015 15:37:12 +0000 (UTC) Subject: [Histonet] Are gloves required when cutting FFPE blocks? In-Reply-To: <667F0DDD9B242E40B448C2CDED9409970A6F8642F0@exch-ms-02.PHCS.ORG> References: <667F0DDD9B242E40B448C2CDED9409970A6F8642F0@exch-ms-02.PHCS.ORG> Message-ID: <868038377.10582834.1448465832777.JavaMail.yahoo@mail.yahoo.com> You should have licked the block in front of them like one supervisor did when questioned about wearing gloves while sectioning! From: "goodwin, diana via Histonet" To: 'Rene J Buesa' ; "'histonet at lists.utsouthwestern.edu'" Sent: Wednesday, November 25, 2015 9:08 AM Subject: Re: [Histonet] Are gloves required when cutting FFPE blocks? We were cited for this by CAP.? I need a specific reference to rebut or comply.? Nothing specific to routine paraffin microtomy in CAP checklists. dg From: Rene J Buesa [mailto:rjbuesa at yahoo.com] Sent: Wednesday, November 25, 2015 10:04 AM To: goodwin, diana; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Are gloves required when cutting FFPE blocks? NOT AT ALL, unless you are sectioning a suspected or known Creutzfeldt-Jakob's disease case. Check CAP regulations. Ren? On Wednesday, November 25, 2015 9:56 AM, "goodwin, diana via Histonet" > wrote: Can anyone provide a reference as to whether or not gloves are required when cutting routine FFPE blocks? Diana Goodwin UMCPP Anatomic Pathology Supervisor Office 609-853-6808 Histology 609-853-16860 This e-mail transmission and any documents attached hereto contain information from Princeton HealthCare System which is confidential and/or legally privileged.? This information is intended only for the use of the individual or entity to which it is addressed, and only for the purpose for which this transmission was made.? Any other use, disclosure, copying, distribution, re-transmission, or the taking of any action in reliance upon the contents of this information is strictly prohibited.? If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, re-transmission or the taking of any action in reliance upon the contents of this information is strictly prohibited, and that this e-mail transmission and accompanying documents should be returned to Princeton HealthCare System immediately.? If you have received this e-mail transmission in error, please notify the sender immediately and destroy all copies of the information contained in this transmission.? The above signature block is intended for identification purposes only and does not constitute affirmation that a binding contract has been created via this e-mail communication unless expressly so stated within the body of this e-mail transmission. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail transmission and any documents attached hereto contain information from Princeton HealthCare System which is confidential and/or legally privileged.? This information is intended only for the use of the individual or entity to which it is addressed, and only for the purpose for which this transmission was made.? Any other use, disclosure, copying, distribution, re-transmission, or the taking of any action in reliance upon the contents of this information is strictly prohibited.? If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, re-transmission or the taking of any action in reliance upon the contents of this information is strictly prohibited, and that this e-mail transmission and accompanying documents should be returned to Princeton HealthCare System immediately.? If you have received this e-mail transmission in error, please notify the sender immediately and destroy all copies of the information contained in this transmission.? The above signature block is intended for identification purposes only and does not constitute affirmation that a binding contract has been created via this e-mail communication unless expressly so stated within the body of this e-mail transmission. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From madeathridge at pastnashville.com Wed Nov 25 09:37:36 2015 From: madeathridge at pastnashville.com (Maryann Deathridge) Date: Wed, 25 Nov 2015 08:37:36 -0700 Subject: [Histonet] DIF on paraffin embedded tissue Message-ID: <2ad258b08df04e578798657c1f439533@pastnashville.com> We have a tissue sample that was processed and paraffin embedded. We URGENTLY need to recover the tissue and perform Immunofluorescence on the sample. Does anyone have a procedure. HELP madeathridge at pastnashville.com From simmca at UPMC.EDU Wed Nov 25 09:52:51 2015 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Wed, 25 Nov 2015 15:52:51 +0000 Subject: [Histonet] Are gloves required when cutting FFPE blocks? In-Reply-To: <667F0DDD9B242E40B448C2CDED9409970A6F8642F0@exch-ms-02.PHCS.ORG> References: <667F0DDD9B242E40B448C2CDED9409970A6F8642EE@exch-ms-02.PHCS.ORG> <2090026182.6706289.1448463859775.JavaMail.yahoo@mail.yahoo.com>, <667F0DDD9B242E40B448C2CDED9409970A6F8642F0@exch-ms-02.PHCS.ORG> Message-ID: <92639D23-D11E-4E80-8B9A-6899431AE72B@UPMC.EDU> Never been cited by cap or clia for not wearing gloves There are hundreds of papers supporting the face that formalin fixed tissues will not spread disease all ER docs cite them when you go in for cutting your finger and ask about transmission CJD cases are the exception Sent from my iPhone > On Nov 25, 2015, at 10:10 AM, goodwin, diana via Histonet wrote: > > We were cited for this by CAP. I need a specific reference to rebut or comply. Nothing specific to routine paraffin microtomy in CAP checklists. > > dg > > From: Rene J Buesa [mailto:rjbuesa at yahoo.com] > Sent: Wednesday, November 25, 2015 10:04 AM > To: goodwin, diana; 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] Are gloves required when cutting FFPE blocks? > > NOT AT ALL, unless you are sectioning a suspected or known Creutzfeldt-Jakob's disease case. Check CAP regulations. > Ren? > > > On Wednesday, November 25, 2015 9:56 AM, "goodwin, diana via Histonet" > wrote: > > Can anyone provide a reference as to whether or not gloves are required when cutting routine FFPE blocks? > > Diana Goodwin > UMCPP > Anatomic Pathology Supervisor > Office 609-853-6808 > Histology 609-853-16860 > > > > This e-mail transmission and any documents attached hereto contain information from Princeton HealthCare System which is confidential and/or legally privileged. This information is intended only for the use of the individual or entity to which it is addressed, and only for the purpose for which this transmission was made. Any other use, disclosure, copying, distribution, re-transmission, or the taking of any action in reliance upon the contents of this information is strictly prohibited. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, re-transmission or the taking of any action in reliance upon the contents of this information is strictly prohibited, and that this e-mail transmission and accompanying documents should be returned to Princeton HealthCare System immediately. If you have received this e-mail transmission in error, please notify the sender immediately and destroy all copies of the information contained in this transmission. The above signature block is intended for identification purposes only and does not constitute affirmation that a binding contract has been created via this e-mail communication unless expressly so stated within the body of this e-mail transmission. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This e-mail transmission and any documents attached hereto contain information from Princeton HealthCare System which is confidential and/or legally privileged. This information is intended only for the use of the individual or entity to which it is addressed, and only for the purpose for which this transmission was made. Any other use, disclosure, copying, distribution, re-transmission, or the taking of any action in reliance upon the contents of this information is strictly prohibited. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, re-transmission or the taking of any action in reliance upon the contents of this information is strictly prohibited, and that this e-mail transmission and accompanying documents should be returned to Princeton HealthCare System immediately. If you have received this e-mail transmission in error, please notify the sender immediately and destroy all copies of the information contained in this transmission. The above signature block is intended for identification purposes only and does not constitute affirmation that a binding contract has been created via this e-mail communication unless expressly so stated within the body of this e-mail transmission. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Wed Nov 25 10:01:57 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 25 Nov 2015 16:01:57 +0000 (UTC) Subject: [Histonet] DIF on paraffin embedded tissue In-Reply-To: <2ad258b08df04e578798657c1f439533@pastnashville.com> References: <2ad258b08df04e578798657c1f439533@pastnashville.com> Message-ID: <1061934882.8577205.1448467317223.JavaMail.yahoo@mail.yahoo.com> No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue?is USELESS.Ren? On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet wrote: We have a tissue sample that was processed and paraffin embedded.? We URGENTLY need to recover the tissue and perform Immunofluorescence on the sample. Does anyone have a procedure.? HELP ? ? ? madeathridge at pastnashville.com? ? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From simmca at UPMC.EDU Wed Nov 25 10:10:27 2015 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Wed, 25 Nov 2015 16:10:27 +0000 Subject: [Histonet] DIF on paraffin embedded tissue In-Reply-To: <1061934882.8577205.1448467317223.JavaMail.yahoo@mail.yahoo.com> References: <2ad258b08df04e578798657c1f439533@pastnashville.com>, <1061934882.8577205.1448467317223.JavaMail.yahoo@mail.yahoo.com> Message-ID: <036D37AA-252B-4486-A09B-6ACF185FB3A5@UPMC.EDU> Useless sample Sorry Sent from my iPhone > On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet wrote: > > No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue is USELESS.Ren? > > > On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet wrote: > > > We have a tissue sample that was processed and paraffin embedded. We > URGENTLY need to recover the tissue and perform Immunofluorescence on the > sample. > Does anyone have a procedure. HELP > > madeathridge at pastnashville.com > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz at premierlab.com Wed Nov 25 10:53:18 2015 From: liz at premierlab.com (Elizabeth Chlipala) Date: Wed, 25 Nov 2015 09:53:18 -0700 Subject: [Histonet] DIF on paraffin embedded tissue Message-ID: <14E2C6176416974295479C64A11CB9AE02851F9CF10C@SBS2K8.premierlab.local> I'm not sure that is the case in the grand scheme of things, it will depend upon the target that you need to stain via immunofluorescence. Technically we perform IF staining on frozen sections primarily because the antigen does not survive formalin fixation. Many people utilize IF techniques on FFPE tissue with good success, we have done it here. There are things you will need to consider and what I suggest below may not work at all. It's up to you if you want to try it or not and you may feel it is not worth the time and energy required to see if it may work on FFPE samples, it could be a lot of work and it may not be successful. 1. You will likely not be able to use your current protocol 2. You will likely need to add an antigen retrieval step 3. You may need to look for a different antibody source (one that survives formalin fixation) 4. The signal needs to be good since formalin fixation will cause autofluorescence Unless I am completely missing something here since two individuals have stated that the sample is useless, maybe there is a better explanation as to why the sample is completely useless. Here is where I am coming from - technically you can perform IF staining on FFPE tissue samples, people do it all of the time, we have done it here, it's a common technique for dual labeling of samples when co-localization is an expected result. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Simmons, Christopher via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, November 25, 2015 9:10 AM To: Rene J Buesa Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] DIF on paraffin embedded tissue Useless sample Sorry Sent from my iPhone > On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet wrote: > > No matter WHO to tell you to do WHAT, for IF purposes, that FFPE > tissue is USELESS.Ren? > > > On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet wrote: > > > We have a tissue sample that was processed and paraffin embedded. We > URGENTLY need to recover the tissue and perform Immunofluorescence on > the sample. > Does anyone have a procedure. HELP > > madeathridge at pastnashville.com > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson at gnf.org Wed Nov 25 10:59:34 2015 From: JWatson at gnf.org (James Watson) Date: Wed, 25 Nov 2015 16:59:34 +0000 Subject: [Histonet] DIF on paraffin embedded tissue In-Reply-To: References: <2ad258b08df04e578798657c1f439533@pastnashville.com>, <1061934882.8577205.1448467317223.JavaMail.yahoo@mail.yahoo.com> <036D37AA-252B-4486-A09B-6ACF185FB3A5@UPMC.EDU> Message-ID: Interesting comments. The use of this sample depends on if the antigen survives fixation and paraffin embedding. About 45-55% of our work is done with immunofluorescence on FFPE slides. we quench the autofluorescence with a CuSO4 treatment. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson at gnf.org -----Original Message----- From: Simmons, Christopher via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, November 25, 2015 8:10 AM To: Rene J Buesa Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] DIF on paraffin embedded tissue Useless sample Sorry Sent from my iPhone > On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet wrote: > > No matter WHO to tell you to do WHAT, for IF purposes, that FFPE > tissue is USELESS.Ren? > > > On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet wrote: > > > We have a tissue sample that was processed and paraffin embedded. We > URGENTLY need to recover the tissue and perform Immunofluorescence on > the sample. > Does anyone have a procedure. HELP > > madeathridge at pastnashville.com > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From steve8438 at gmail.com Wed Nov 25 11:03:46 2015 From: steve8438 at gmail.com (Steven Mello) Date: Wed, 25 Nov 2015 12:03:46 -0500 Subject: [Histonet] DIF on paraffin embedded tissue In-Reply-To: <036D37AA-252B-4486-A09B-6ACF185FB3A5@UPMC.EDU> References: <2ad258b08df04e578798657c1f439533@pastnashville.com> <1061934882.8577205.1448467317223.JavaMail.yahoo@mail.yahoo.com> <036D37AA-252B-4486-A09B-6ACF185FB3A5@UPMC.EDU> Message-ID: No way...no how Sorry! Sent from my iPad > On Nov 25, 2015, at 11:10 AM, Simmons, Christopher via Histonet wrote: > > Useless sample > Sorry > > Sent from my iPhone > >> On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet wrote: >> >> No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue is USELESS.Ren? >> >> >> On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet wrote: >> >> >> We have a tissue sample that was processed and paraffin embedded. We >> URGENTLY need to recover the tissue and perform Immunofluorescence on the >> sample. >> Does anyone have a procedure. HELP >> >> madeathridge at pastnashville.com >> >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marcus.green at oncology.ox.ac.uk Wed Nov 25 11:23:21 2015 From: marcus.green at oncology.ox.ac.uk (Marcus Green) Date: Wed, 25 Nov 2015 17:23:21 +0000 Subject: [Histonet] DIF on paraffin embedded tissue In-Reply-To: <14E2C6176416974295479C64A11CB9AE02851F9CF10C@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE02851F9CF10C@SBS2K8.premierlab.local> Message-ID: <669331A24821FE4491665E8DFD47CF22BFD156@MBX06.ad.oak.ox.ac.uk> I, agreeing with Liz, would suggest looking out a IF protocol for FFPE tissues - I've recently concluded a validation of PLA (proximity ligation assay) in: cells in culture, FFPE cell pellets and FFPE xenografts. The signal was consistently there (although a drop in expression - from fixative reasons as well as culture conditions). Background is the biggest issue for IF using FFPE so make sure to run some comprehensive controls. You will need to run a Deparafinisation, Dehydration and Rehydration as you would IHC (3mins xylene x2, 3mins 100% EtOH, 3mins 70%EtOH and 3mins 50%EtOH - or similar) before running an Antigen Retrieval - if you have this optimised for IHC use something similar (pH and time). I would also consider a permeabilization step and if you need to optimise for background (Sudan black wash). If, however the question is can you reinstate a tissue back to an unfixed tissue for freeze processing then no you can't. hope this helps, kind regards, Marcus ________________________________________ From: Elizabeth Chlipala via Histonet [histonet at lists.utsouthwestern.edu] Sent: 25 November 2015 16:53 To: Simmons, Christopher; Rene J Buesa Cc: 'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu) Subject: Re: [Histonet] DIF on paraffin embedded tissue I'm not sure that is the case in the grand scheme of things, it will depend upon the target that you need to stain via immunofluorescence. Technically we perform IF staining on frozen sections primarily because the antigen does not survive formalin fixation. Many people utilize IF techniques on FFPE tissue with good success, we have done it here. There are things you will need to consider and what I suggest below may not work at all. It's up to you if you want to try it or not and you may feel it is not worth the time and energy required to see if it may work on FFPE samples, it could be a lot of work and it may not be successful. 1. You will likely not be able to use your current protocol 2. You will likely need to add an antigen retrieval step 3. You may need to look for a different antibody source (one that survives formalin fixation) 4. The signal needs to be good since formalin fixation will cause autofluorescence Unless I am completely missing something here since two individuals have stated that the sample is useless, maybe there is a better explanation as to why the sample is completely useless. Here is where I am coming from - technically you can perform IF staining on FFPE tissue samples, people do it all of the time, we have done it here, it's a common technique for dual labeling of samples when co-localization is an expected result. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Simmons, Christopher via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, November 25, 2015 9:10 AM To: Rene J Buesa Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] DIF on paraffin embedded tissue Useless sample Sorry Sent from my iPhone > On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet wrote: > > No matter WHO to tell you to do WHAT, for IF purposes, that FFPE > tissue is USELESS.Ren? > > > On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet wrote: > > > We have a tissue sample that was processed and paraffin embedded. We > URGENTLY need to recover the tissue and perform Immunofluorescence on > the sample. > Does anyone have a procedure. HELP > > madeathridge at pastnashville.com > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly at merck.com Wed Nov 25 11:28:12 2015 From: brett_connolly at merck.com (Connolly, Brett M) Date: Wed, 25 Nov 2015 12:28:12 -0500 Subject: [Histonet] DIF on paraffin embedded tissue In-Reply-To: <669331A24821FE4491665E8DFD47CF22BFD156@MBX06.ad.oak.ox.ac.uk> References: <14E2C6176416974295479C64A11CB9AE02851F9CF10C@SBS2K8.premierlab.local> <669331A24821FE4491665E8DFD47CF22BFD156@MBX06.ad.oak.ox.ac.uk> Message-ID: Also agree. Lots of people do it. Search PubMed, J. Histo Cyto. If you have the appropriate filters, using Cy dye secondary's can help move away from any autofluorescence. Brett Brett M. Connolly, Ph.D. Prin. Scientist, Translational Biomarkers - Imaging Merck & Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_connolly at merck.com T- 215-652-2501 F- 215-993-6803 -----Original Message----- From: Marcus Green via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, November 25, 2015 12:23 PM To: Elizabeth Chlipala; Simmons, Christopher; Rene J Buesa Cc: 'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu) Subject: Re: [Histonet] DIF on paraffin embedded tissue I, agreeing with Liz, would suggest looking out a IF protocol for FFPE tissues - I've recently concluded a validation of PLA (proximity ligation assay) in: cells in culture, FFPE cell pellets and FFPE xenografts. The signal was consistently there (although a drop in expression - from fixative reasons as well as culture conditions). Background is the biggest issue for IF using FFPE so make sure to run some comprehensive controls. You will need to run a Deparafinisation, Dehydration and Rehydration as you would IHC (3mins xylene x2, 3mins 100% EtOH, 3mins 70%EtOH and 3mins 50%EtOH - or similar) before running an Antigen Retrieval - if you have this optimised for IHC use something similar (pH and time). I would also consider a permeabilization step and if you need to optimise for background (Sudan black wash). If, however the question is can you reinstate a tissue back to an unfixed tissue for freeze processing then no you can't. hope this helps, kind regards, Marcus ________________________________________ From: Elizabeth Chlipala via Histonet [histonet at lists.utsouthwestern.edu] Sent: 25 November 2015 16:53 To: Simmons, Christopher; Rene J Buesa Cc: 'histonet at lists.utsouthwestern.edu' (histonet at lists.utsouthwestern.edu) Subject: Re: [Histonet] DIF on paraffin embedded tissue I'm not sure that is the case in the grand scheme of things, it will depend upon the target that you need to stain via immunofluorescence. Technically we perform IF staining on frozen sections primarily because the antigen does not survive formalin fixation. Many people utilize IF techniques on FFPE tissue with good success, we have done it here. There are things you will need to consider and what I suggest below may not work at all. It's up to you if you want to try it or not and you may feel it is not worth the time and energy required to see if it may work on FFPE samples, it could be a lot of work and it may not be successful. 1. You will likely not be able to use your current protocol 2. You will likely need to add an antigen retrieval step 3. You may need to look for a different antibody source (one that survives formalin fixation) 4. The signal needs to be good since formalin fixation will cause autofluorescence Unless I am completely missing something here since two individuals have stated that the sample is useless, maybe there is a better explanation as to why the sample is completely useless. Here is where I am coming from - technically you can perform IF staining on FFPE tissue samples, people do it all of the time, we have done it here, it's a common technique for dual labeling of samples when co-localization is an expected result. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Simmons, Christopher via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, November 25, 2015 9:10 AM To: Rene J Buesa Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] DIF on paraffin embedded tissue Useless sample Sorry Sent from my iPhone > On Nov 25, 2015, at 11:02 AM, Rene J Buesa via Histonet wrote: > > No matter WHO to tell you to do WHAT, for IF purposes, that FFPE > tissue is USELESS.Ren? > > > On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet wrote: > > > We have a tissue sample that was processed and paraffin embedded. We > URGENTLY need to recover the tissue and perform Immunofluorescence on > the sample. > Does anyone have a procedure. HELP > > madeathridge at pastnashville.com > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. 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From Ronald.Houston at nationwidechildrens.org Wed Nov 25 11:43:41 2015 From: Ronald.Houston at nationwidechildrens.org (Houston, Ronald) Date: Wed, 25 Nov 2015 17:43:41 +0000 Subject: [Histonet] DIF on paraffin embedded tissue In-Reply-To: <1061934882.8577205.1448467317223.JavaMail.yahoo@mail.yahoo.com> References: <2ad258b08df04e578798657c1f439533@pastnashville.com> <1061934882.8577205.1448467317223.JavaMail.yahoo@mail.yahoo.com> Message-ID: Obviously it depends on what you are looking at, but I have had much success with DIF on paraffin sections Ronnie Houston, MS HT(ASCP)QIHC FIBMS Anatomic Pathology Manager 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston at nationwidechildrens.org www.NationwideChildrens.org "Without continual growth and progress, such words as improvement, achievement, and success have no meaning." ~ Ben Franklin -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, November 25, 2015 11:02 AM To: madeathridge at pastnashville.com; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] DIF on paraffin embedded tissue No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue?is USELESS.Ren? On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet wrote: We have a tissue sample that was processed and paraffin embedded.? We URGENTLY need to recover the tissue and perform Immunofluorescence on the sample. Does anyone have a procedure.? HELP ? ? ? madeathridge at pastnashville.com? ? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQIGaQ&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=lUwE5OrviOlSTXqQIjMclJQmIL0MLN3P_J4Xb6gbNhM&s=Kd_zUtcfY-o9DE5GFw2BadBo4NSkqN11Ut0kLm8gNoo&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQIGaQ&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=lUwE5OrviOlSTXqQIjMclJQmIL0MLN3P_J4Xb6gbNhM&s=Kd_zUtcfY-o9DE5GFw2BadBo4NSkqN11Ut0kLm8gNoo&e= From simmca at UPMC.EDU Wed Nov 25 11:47:16 2015 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Wed, 25 Nov 2015 17:47:16 +0000 Subject: [Histonet] DIF on paraffin embedded tissue In-Reply-To: References: <2ad258b08df04e578798657c1f439533@pastnashville.com> <1061934882.8577205.1448467317223.JavaMail.yahoo@mail.yahoo.com>, Message-ID: <58B5C593-A065-42BC-B9CB-630E3F12B9A5@UPMC.EDU> By all means share your protocols plz Sent from my iPhone > On Nov 25, 2015, at 12:44 PM, Houston, Ronald via Histonet wrote: > > Obviously it depends on what you are looking at, but I have had much success with DIF on paraffin sections > > > Ronnie Houston, MS HT(ASCP)QIHC FIBMS > Anatomic Pathology Manager > 700 Children's Drive > Columbus, OH 43205 > (P) 614-722-5450 > (F) 614-722-2899 > ronald.houston at nationwidechildrens.org > www.NationwideChildrens.org > > "Without continual growth and progress, such words as improvement, achievement, and success have no meaning." > ~ Ben Franklin > > > > -----Original Message----- > From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, November 25, 2015 11:02 AM > To: madeathridge at pastnashville.com; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] DIF on paraffin embedded tissue > > No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue is USELESS.Ren? > > > On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet wrote: > > > We have a tissue sample that was processed and paraffin embedded. We URGENTLY need to recover the tissue and perform Immunofluorescence on the sample. > Does anyone have a procedure. HELP > > madeathridge at pastnashville.com > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQIGaQ&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=lUwE5OrviOlSTXqQIjMclJQmIL0MLN3P_J4Xb6gbNhM&s=Kd_zUtcfY-o9DE5GFw2BadBo4NSkqN11Ut0kLm8gNoo&e= > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQIGaQ&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=lUwE5OrviOlSTXqQIjMclJQmIL0MLN3P_J4Xb6gbNhM&s=Kd_zUtcfY-o9DE5GFw2BadBo4NSkqN11Ut0kLm8gNoo&e= > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tejohnson at genoptix.com Wed Nov 25 12:29:59 2015 From: tejohnson at genoptix.com (Teri Johnson) Date: Wed, 25 Nov 2015 18:29:59 +0000 Subject: [Histonet] DIF on paraffin embedded tissue Message-ID: Maryann, Whoa, bad day. I'm sorry for you and for the patient. As for whether the testing usually performed in frozen section by Direct ImmunoFluorescence can be successfully done on FFPE, Liz Chlipala is probably the closest to answering this. This was likely a kidney or skin biopsy. The panel is likely something like IgG, IgA, IgM, C3, Fibrinogen and maybe another marker or so. Can it be done? I would say not with any degree of confidence unless this was extensively tested side-by-side with frozen vs paraffin embedded tissues. The IHC stain techniques would be radically different. This would take a lot of time. Now, if you were successful in providing these assays that you have validated in your laboratory (I shudder to think what that would entail), would the pathologist be comfortable signing out a case such as this? Would the clinician be comfortable treating the patient based on these results? Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From agleiberman at buffalobiolabs.com Wed Nov 25 12:39:52 2015 From: agleiberman at buffalobiolabs.com (Anatoli Gleiberman) Date: Wed, 25 Nov 2015 18:39:52 +0000 Subject: [Histonet] DIF on paraffin embedded tissue In-Reply-To: <1061934882.8577205.1448467317223.JavaMail.yahoo@mail.yahoo.com> References: <2ad258b08df04e578798657c1f439533@pastnashville.com> <1061934882.8577205.1448467317223.JavaMail.yahoo@mail.yahoo.com> Message-ID: Rene, For many (but not for all) antigens with proper fixation (not too long) and retrieval IF on FFPE sections works fine. Here is short list of what we are doing routinely: all pituitary hormones, smooth muscle actin, alpha-fetoprotein, BJP, Ki67, p65 NFkB, CD3, IgG subclasses, IgM, B220, SOD2, POU domain transcription factors. Of course, fresh frozen samples or frozen sections from formaldehyde fixed samples give much better results, but still - if you need to do it on FFPE it is possible. Anatoli Gleiberman, PhD Director of Histopathology Buffalo Biolabs LLC, 73 High Street Buffalo, NY, 14203 Phone:716-849-6810x354 e-mail:agleiberman at buffalobiolab.com -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, November 25, 2015 11:02 AM To: madeathridge at pastnashville.com; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] DIF on paraffin embedded tissue No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue?is USELESS.Ren? On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet wrote: We have a tissue sample that was processed and paraffin embedded.? We URGENTLY need to recover the tissue and perform Immunofluorescence on the sample. Does anyone have a procedure.? HELP ? ? ? madeathridge at pastnashville.com? ? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz at premierlab.com Wed Nov 25 13:55:11 2015 From: liz at premierlab.com (Elizabeth Chlipala) Date: Wed, 25 Nov 2015 12:55:11 -0700 Subject: [Histonet] DIF on paraffin embedded tissue In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02851F9CF119@SBS2K8.premierlab.local> Teri Excellent point and to add to that most IF techniques that are employed on FFPE are not direct they are indirect and consist of an unlabeled primary and then a secondary that is conjugated with an alexa fluor or something similar at least that is how we approach IF on FFPE tissues here. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Teri Johnson via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, November 25, 2015 11:30 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] DIF on paraffin embedded tissue Maryann, Whoa, bad day. I'm sorry for you and for the patient. As for whether the testing usually performed in frozen section by Direct ImmunoFluorescence can be successfully done on FFPE, Liz Chlipala is probably the closest to answering this. This was likely a kidney or skin biopsy. The panel is likely something like IgG, IgA, IgM, C3, Fibrinogen and maybe another marker or so. Can it be done? I would say not with any degree of confidence unless this was extensively tested side-by-side with frozen vs paraffin embedded tissues. The IHC stain techniques would be radically different. This would take a lot of time. Now, if you were successful in providing these assays that you have validated in your laboratory (I shudder to think what that would entail), would the pathologist be comfortable signing out a case such as this? Would the clinician be comfortable treating the patient based on these results? Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pwnoyce at gmail.com Wed Nov 25 18:26:32 2015 From: pwnoyce at gmail.com (Peter Noyce) Date: Thu, 26 Nov 2015 11:26:32 +1100 Subject: [Histonet] Toluidine blue stain fo plant tissue Message-ID: <000201d127e1$1d4e9870$57ebc950$@gmail.com> Could anyone give me the concentration (? 0.1-0.05%) and the exact technique/protocol to use. I am staining shoot apical meristems not leaf or root. Regards Peter Noyce PhD student. From blayjorge at gmail.com Wed Nov 25 20:09:10 2015 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Wed, 25 Nov 2015 21:09:10 -0500 Subject: [Histonet] Stains specific to nervous vs. endocrine tissue on insects Message-ID: Dear Histonetters: Could I be directed to stains specific to nervous vs. endocrine tissue on insects? Gracias, sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From Lisa.White3 at va.gov Fri Nov 27 07:50:07 2015 From: Lisa.White3 at va.gov (White, Lisa M.) Date: Fri, 27 Nov 2015 08:50:07 -0500 Subject: [Histonet] : Are gloves required when cutting FFPE blocks? Message-ID: <2B2ECF33934F5D4996D8BE03EFDF39760C478919@VHAV09MSGA3.v09.med.va.gov> I am not aware of any regulation to use gloves to section. However I do use them to section on the microtome, I got really tired of picking human out from under my fingernails. The other 4 HT that I work with do not use them on standard microtome sections. CJD would be the only exception, but then again the all of the processing equipment would then be a problem also. From blayjorge at gmail.com Fri Nov 27 10:58:07 2015 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Fri, 27 Nov 2015 11:58:07 -0500 Subject: [Histonet] Recommendations of places to purchase two stains and question on protocol Message-ID: Dear Histonetters: I am trying to purchase two stains: 1. Cresil etch violet (for nervous tissue) - https://en.wikipedia.org/wiki/Cresyl_violet 2. Mallory azan (for encocrine tissue) - https://www.emsdiasum.com/microscopy/technical/datasheet/26450.aspx , https://en.wikipedia.org/wiki/Heidenhain%27s_AZAN_trichrome_stain Have searched the web and rather than gamble, wish to know if I could receive recommendations on good places to purchase this type of reagent. Good customer service and affordable prices a must. In both cases, do you know if these stains work on tissues preserved in ethanol (70%), some of the tissues could have been preserved for years. If you have constructive feedback, please feel free to email me at blayjorge at gmail.com Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From liz at premierlab.com Fri Nov 27 11:28:13 2015 From: liz at premierlab.com (Elizabeth Chlipala) Date: Fri, 27 Nov 2015 10:28:13 -0700 Subject: [Histonet] Recommendations of places to purchase two stains and question on protocol In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02851F9CF11D@SBS2K8.premierlab.local> Jorge I can comment on the cresyl etch violet - we actually purchased cresyl violet acetate from MP Biomedicals. This particular chemical has not been certified by the biological stain commission, if you are looking for a dye that has been certified then I would go to Sigma-Aldrich - they also supply this chemical. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Jorge A. Santiago-Blay via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, November 27, 2015 9:58 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Recommendations of places to purchase two stains and question on protocol Dear Histonetters: I am trying to purchase two stains: 1. Cresil etch violet (for nervous tissue) - https://en.wikipedia.org/wiki/Cresyl_violet 2. Mallory azan (for encocrine tissue) - https://www.emsdiasum.com/microscopy/technical/datasheet/26450.aspx , https://en.wikipedia.org/wiki/Heidenhain%27s_AZAN_trichrome_stain Have searched the web and rather than gamble, wish to know if I could receive recommendations on good places to purchase this type of reagent. Good customer service and affordable prices a must. In both cases, do you know if these stains work on tissues preserved in ethanol (70%), some of the tissues could have been preserved for years. If you have constructive feedback, please feel free to email me at blayjorge at gmail.com Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mills at 3scan.com Sat Nov 28 09:46:52 2015 From: mills at 3scan.com (Caroline Miller) Date: Sat, 28 Nov 2015 07:46:52 -0800 Subject: [Histonet] : Are gloves required when cutting FFPE blocks? In-Reply-To: <2B2ECF33934F5D4996D8BE03EFDF39760C478919@VHAV09MSGA3.v09.med.va.gov> References: <2B2ECF33934F5D4996D8BE03EFDF39760C478919@VHAV09MSGA3.v09.med.va.gov> Message-ID: I tend to leave my squames on the slide, so I always wear gloves too mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 > On Nov 27, 2015, at 5:50 AM, White, Lisa M. via Histonet wrote: > > I am not aware of any regulation to use gloves to section. > > > > However I do use them to section on the microtome, I got really tired of > picking human out from under my fingernails. > > The other 4 HT that I work with do not use them on standard microtome > sections. > > > > CJD would be the only exception, but then again the all of the > processing equipment would then be a problem also. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 at gmail.com Sat Nov 28 10:07:34 2015 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Sat, 28 Nov 2015 08:07:34 -0800 Subject: [Histonet] Hourly wage for trainee in CA Message-ID: <9509ADC7-6421-45C0-B3EB-E77E8BEE3B50@gmail.com> Hello all my histology friends in California: 1st I hope you all had a wonderful Thanksgiving! I would like to know what the hourly rate of a new histologist with a HT certification is making. I would very much appreciate this information. We are offering a training position to a local individual who doesn't have a degree, HT or HTL certification, or recent experience (last worked in histology in 1991). I want to see if our offer of $20.00 an hour plus benefit's was out of line. I would prefer to have a certified HT, but no one has answered our ads. Best regards, Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com Tele: (831) 375-3577 X117 From rsrichmond at gmail.com Sat Nov 28 13:39:36 2015 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 28 Nov 2015 14:39:36 -0500 Subject: [Histonet] Cresyl echt violet and Mallory azan Message-ID: Jorge A. Santiago-Blay, PhD (where?) asks about availability of cresyl echt violet and "Mallory azan". Cresyl violet acetate - also known as cresyl echt violet or cresyl fast violet - doesn't have a Colour Index number - I'd want to be certain to buy a product certified by the Biological Stain Commission, such as Sigma-Aldrich's http://www.sigmaaldrich.com/catalog/product/sigma/c5042?lang=en®ion=US "Mallory azan" (actually Heidenhain azan) was a technique rather than a stain - "azan" was an acronym for the two dyes azocarmine and aniline blue. This century-old stain was considered obsolete when I was a resident 50 years ago, and Lillie (3rd ed. 1965) noted that he'd never tried it because it was so time-consuming. What you probably need is a good blue trichrome stain such as http://www.sigmaaldrich.com/catalog/product/sigma/ht15?lang=en®ion=US Bob Richmond Samurai Pathologist Maryville TN From bszpunar at umail.iu.edu Sat Nov 28 18:36:46 2015 From: bszpunar at umail.iu.edu (Bryan Szpunar) Date: Sat, 28 Nov 2015 19:36:46 -0500 Subject: [Histonet] Hourly wage for trainee in CA Message-ID: Hi Akemi, I am not in California, but if I were hiring for a *certified *HT/HTL in your region I would think you are a little low at $20/hr. Check out the most recent ASCP wage survey for some reference. Since you said you are hiring as a training position I think I feel pretty comfortable with your offer. Bryan > Date: Sat, 28 Nov 2015 08:07:34 -0800 > From: Eileen Akemi Allison > To: Histonet > Subject: [Histonet] Hourly wage for trainee in CA > Message-ID: <9509ADC7-6421-45C0-B3EB-E77E8BEE3B50 at gmail.com> > Content-Type: text/plain; charset=us-ascii > > Hello all my histology friends in California: > 1st I hope you all had a wonderful Thanksgiving! I would like to know what > the hourly rate of a new histologist with a HT certification is making. I > would very much appreciate this information. We are offering a training > position to a local individual who doesn't have a degree, HT or HTL > certification, or recent experience (last worked in histology in 1991). I > want to see if our offer of $20.00 an hour plus benefit's was out of line. > I would prefer to have a certified HT, but no one has answered our ads. > > Best regards, > > Akemi Allison BS, HT/HTL (ASCP) > Pathology Manager > Monterey Bay GI Consultants Laboratory > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > W: Email: aallison at montereygi.com > H: Email: akemiat3377 at gmail.com > Tele: (831) 375-3577 X117 > > From LRaff at uropartners.com Sun Nov 29 08:06:24 2015 From: LRaff at uropartners.com (Lester Raff MD) Date: Sun, 29 Nov 2015 14:06:24 +0000 Subject: [Histonet] Sunday Morning-not work related Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF0B535FAC@COLOEXCH01.uropartners.local> Something to read as you recover from or extend your holiday weekend. http://tinyurl.com/down1129 or if your browser won't allow tinyurl http://www.chicagonow.com/downsize-maybe/2015/11/menagerie/ Les Raff From JMacDonald at mtsac.edu Sun Nov 29 22:14:33 2015 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Sun, 29 Nov 2015 20:14:33 -0800 Subject: [Histonet] Hourly wage for trainee in CA In-Reply-To: <9509ADC7-6421-45C0-B3EB-E77E8BEE3B50@gmail.com> References: <9509ADC7-6421-45C0-B3EB-E77E8BEE3B50@gmail.com> Message-ID: Akemi, I think this offer is low for a certified HT/HTL, particularly in that area. I have had graduates go up to Northern CA and the minimum starting wage that they have accepted is $25. These are brand new graduates, with minimal experience, but I don't believe their titles are "trainees". Jennifer From: Eileen Akemi Allison via Histonet To: Histonet Date: 11/28/2015 08:08 AM Subject: [Histonet] Hourly wage for trainee in CA Hello all my histology friends in California: 1st I hope you all had a wonderful Thanksgiving! I would like to know what the hourly rate of a new histologist with a HT certification is making. I would very much appreciate this information. We are offering a training position to a local individual who doesn't have a degree, HT or HTL certification, or recent experience (last worked in histology in 1991). I want to see if our offer of $20.00 an hour plus benefit's was out of line. I would prefer to have a certified HT, but no one has answered our ads. Best regards, Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com Tele: (831) 375-3577 X117 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 at gmail.com Mon Nov 30 00:51:36 2015 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Sun, 29 Nov 2015 22:51:36 -0800 Subject: [Histonet] Hourly wage for trainee in CA In-Reply-To: References: <9509ADC7-6421-45C0-B3EB-E77E8BEE3B50@gmail.com> Message-ID: Jennifer: I think you misread the inquiry. I would love to get a new graduate at $25.00 to $28.00 and hour. I sent you an email to see if you had any candidates, but you did not respond. This position is considered more of a histology assistant since this person doesn't have a degree (only HS), no HT or HTL certification, or recent experience (last worked in histology in 1991). In my opinion, I think our offer of $20.00 an hour plus a handsome benefit's pack is not out of line. It will take quite a bit of training to bring her up to speed. She also won't be eligible to sit for her HT certification because she only has a HS education. Akemi > On Nov 29, 2015, at 8:14 PM, Jennifer MacDonald wrote: > > Akemi, > I think this offer is low for a certified HT/HTL, particularly in that area. I have had graduates go up to Northern CA and the minimum starting wage that they have accepted is $25. These are brand new graduates, with minimal experience, but I don't believe their titles are "trainees". > Jennifer > > > > From: Eileen Akemi Allison via Histonet > To: Histonet > Date: 11/28/2015 08:08 AM > Subject: [Histonet] Hourly wage for trainee in CA > > > > Hello all my histology friends in California: > 1st I hope you all had a wonderful Thanksgiving! I would like to know what the hourly rate of a new histologist with a HT certification is making. I would very much appreciate this information. We are offering a training position to a local individual who doesn't have a degree, HT or HTL certification, or recent experience (last worked in histology in 1991). I want to see if our offer of $20.00 an hour plus benefit's was out of line. I would prefer to have a certified HT, but no one has answered our ads. > > Best regards, > > Akemi Allison BS, HT/HTL (ASCP) > Pathology Manager > Monterey Bay GI Consultants Laboratory > 23 Upper Ragsdale Drive, Suite 200 > Monterey, CA 93940 > W: Email: aallison at montereygi.com > > H: Email: akemiat3377 at gmail.com > > Tele: (831) 375-3577 X117 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From hans at histologistics.com Mon Nov 30 10:38:52 2015 From: hans at histologistics.com (Hans B Snyder) Date: Mon, 30 Nov 2015 11:38:52 -0500 Subject: [Histonet] Duraedge (Black) help Message-ID: Hello All, I recently switched from Duraedge (red) to Duraedge (black) blades. This has caused cutting problems on 1 microtome, our Leica RM 2235 microtome but no others. The sections are now thick and thin, compressed or lengthwise ribbon splits. I tried changing the blade holder angle, 6 forward and 6 back positions with no luck. After trying 12 different knife angles with no luck, I had someone PM it and it's still not working. The original red blades still work in the microtome and I don't understand how only 1 microtome does not work with these blades. Instead of buying a whole new case of red blades just for this 1 microtome, I thought you might have some insight that could help. What should I do? Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans at histologistics.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From JRobinson at pathology-associates.com Mon Nov 30 10:43:42 2015 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Mon, 30 Nov 2015 16:43:42 +0000 Subject: [Histonet] Digital Imaging on breast panels Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C90A0AE44@PAEXCH1.PathologyAssociates.local> Good Morning and Happy Holidays to all my fellow Histotechs- We are having problems with our Ventana iScan digital imaging system. It breaks down frequently and is causing extreme frustration for my pathologists. It has gotten so bad we are trying to revive the old Ventana VIAS and use it instead. The problem with using the VIAS is that Ventana will no longer support it as far as service (they are happy to sell us the cards). My lab manager has asked me to reach out to all of you experienced techs to see what you are using for digital imaging for breast prognostic markers and what kind of experiences (good or bad) you have had with your system. Thank you in advance! Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From rjbuesa at yahoo.com Mon Nov 30 11:03:32 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 30 Nov 2015 17:03:32 +0000 (UTC) Subject: [Histonet] Duraedge (Black) help In-Reply-To: References: Message-ID: <101089747.10243561.1448903012573.JavaMail.yahoo@mail.yahoo.com> Extremely rare this problem because almost always thick/thin sections has nothing to do with the blades but with the microtome that is unable to hold the blade firmly.Are the blades in this microtome "thinner" than the others used in it previously?If they are, you could add a small piece of aluminum foil surrounding the thinner blade to make it thicker and perhaps enabling the blade holder in this microtome to hold them tighter. Just a thought.Ren? On Monday, November 30, 2015 11:54 AM, Hans B Snyder via Histonet wrote: Hello All, I recently switched from Duraedge (red) to Duraedge (black) blades.? This has caused cutting problems on 1 microtome, our Leica RM 2235 microtome but no others.? The sections are now thick and thin, compressed or lengthwise ribbon splits. I tried changing the blade holder angle, 6 forward and 6 back positions with no luck.? After trying 12 different knife angles with no luck, I had someone PM it and it's still not working. The original red blades still work in the microtome and I don't understand how only 1 microtome does not work with these blades.? Instead of buying a whole new case of red blades just for this 1 microtome, I thought you might have some insight that could help. What should I do? Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans at histologistics.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fourfonners at yahoo.com Mon Nov 30 12:20:56 2015 From: fourfonners at yahoo.com (Sheila Fonner) Date: Mon, 30 Nov 2015 18:20:56 +0000 (UTC) Subject: [Histonet] Leica Bond Detection Kits References: <187242780.12062816.1448907656770.JavaMail.yahoo.ref@mail.yahoo.com> Message-ID: <187242780.12062816.1448907656770.JavaMail.yahoo@mail.yahoo.com> Hello everyone, hope this Monday is treating you well. I am in dire need of some assistance. We are a dermpath? lab in Knoxville, TN using the Leica Bond for our IHC. We have been unable to acquire our Refine Red Detection Kits due to some technical difficulty. I am looking for anyone who currently uses the refine red and would be willing and/or able to loan us 2 red kits until we receive our new lot. I have 9 on order but I am extremely backed up. We would pay for all shipping and would replace them asap.?Thank you for any help you can provide.Sheila HT, (ASCP)DPPKnoxville, TN From LSebree at uwhealth.org Mon Nov 30 15:48:05 2015 From: LSebree at uwhealth.org (Sebree Linda A) Date: Mon, 30 Nov 2015 21:48:05 +0000 Subject: [Histonet] Looking for a referral lab offering TFE-3 by FISH: human FFPE specimen Message-ID: <77DD817201982748BC67D7960F2F76AF1B0C1E05@UWHC-MBX13.uwhis.hosp.wisc.edu> Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174