From Joyce.Weems at emoryhealthcare.org Fri May 1 08:13:18 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Fri, 1 May 2015 13:13:18 +0000 Subject: [Histonet] IHC billing question In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> Message-ID: I do it every day - change every first to 88342. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, April 30, 2015 5:33 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC billing question Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From plucas at biopath.org Fri May 1 09:16:56 2015 From: plucas at biopath.org (Paula Lucas) Date: Fri, 1 May 2015 07:16:56 -0700 Subject: [Histonet] GMS Question Message-ID: Hello, I think I already know the answer but I'm not sure why so if someone can help me understand the theory behind it, I would greatly appreciate it. Currently, we use the Richard Allen kit for the GMS stain and it uses Periodic Acid as the 1st step. We use a control tissue from a case we had that was positive for fungus and it's a fungus ball from the Rt Maxillary. We ran a test for fungus on a different and current case of the same tissue (different patient): Rt Maxillary sinus. The control tissue did work, but the patient's tissue did not, so the doctor ordered a PAS for fungus and this clearly showed the fungal elements nicely. My question is why would the control and patient tissue have different results when they are both fungus balls from the same specimen source? Thanks in advance, Paula Lab Manager Bio-Path Medical Group From TGoins at mt.gov Fri May 1 09:41:16 2015 From: TGoins at mt.gov (Goins, Tresa) Date: Fri, 1 May 2015 14:41:16 +0000 Subject: [Histonet] Question In-Reply-To: <8464cf90c79445098748ffb0e6c04f36@evcspmbx03.ads.northwestern.edu> References: <8464cf90c79445098748ffb0e6c04f36@evcspmbx03.ads.northwestern.edu> Message-ID: All that matters here is the final concentration of the reagent - it doesn't matter what stock you start with if you calculate the dilution. Adding 1.25 ml to 1000 ml is diluted by a factor of .00125 so 10N x .00125 = .0125 N final concentration. If using a 1 N stock solution, just add 10X the volume or 12.5 ml 1 N stock to 987.5 ml water. Hope this helps, Tresa -----Original Message----- From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, April 30, 2015 1:35 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Question All, I have a procedure here that call for and I quote "1.25 ml NaOH 10N in 1L of water." I know how to get 1 N, but how do I get 10. Having rarely hd the opportunity to make many Normal solutions ,my brain is not computing. Is it an error? Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward at wakehealth.edu Fri May 1 09:41:36 2015 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Fri, 1 May 2015 14:41:36 +0000 Subject: [Histonet] IHC billing question In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org>, Message-ID: That's what we do as well. Martha Ward Wake Forest Baptist Health ________________________________________ From: Weems, Joyce K. [Joyce.Weems at emoryhealthcare.org] Sent: Friday, May 01, 2015 9:13 AM To: 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing question I do it every day - change every first to 88342. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, April 30, 2015 5:33 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC billing question Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff at rwjms.rutgers.edu Fri May 1 10:15:40 2015 From: mcauliff at rwjms.rutgers.edu (Geoff) Date: Fri, 01 May 2015 11:15:40 -0400 Subject: [Histonet] Question In-Reply-To: References: <8464cf90c79445098748ffb0e6c04f36@evcspmbx03.ads.northwestern.edu> Message-ID: <5543989C.8030602@umdnj.edu> Huh? Take a solution more dilute than you want it and dilute it more? Geoff On 5/1/2015 10:41 AM, Goins, Tresa wrote: > All that matters here is the final concentration of the reagent - it doesn't matter what stock you start with if you calculate the dilution. > Adding 1.25 ml to 1000 ml is diluted by a factor of .00125 so 10N x .00125 = .0125 N final concentration. > If using a 1 N stock solution, just add 10X the volume or 12.5 ml 1 N stock to 987.5 ml water. > > Hope this helps, > > Tresa > > -----Original Message----- > From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Bernice Frederick > Sent: Thursday, April 30, 2015 1:35 PM > To: Histonet at lists.utsouthwestern.edu > Subject: [Histonet] Question > > All, > I have a procedure here that call for and I quote "1.25 ml NaOH 10N in 1L of water." I know how to get 1 N, but how do I get 10. Having rarely hd the opportunity to make many Normal solutions ,my brain is not computing. Is it an error? > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick at northwestern.edu > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff at rwjms.rutgers.edu ********************************************** From TGoins at mt.gov Fri May 1 10:29:17 2015 From: TGoins at mt.gov (Goins, Tresa) Date: Fri, 1 May 2015 15:29:17 +0000 Subject: [Histonet] Question In-Reply-To: <5543989C.8030602@umdnj.edu> References: <8464cf90c79445098748ffb0e6c04f36@evcspmbx03.ads.northwestern.edu> <5543989C.8030602@umdnj.edu> Message-ID: The final concentration of 1.25 ml 10N NaOH into 1000 ml water is the same as: 12.5 ml 1N NaOH into 987.5 ml water. -----Original Message----- From: Geoff [mailto:mcauliff at rwjms.rutgers.edu] Sent: Friday, May 01, 2015 9:16 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Question Huh? Take a solution more dilute than you want it and dilute it more? Geoff On 5/1/2015 10:41 AM, Goins, Tresa wrote: > All that matters here is the final concentration of the reagent - it doesn't matter what stock you start with if you calculate the dilution. > Adding 1.25 ml to 1000 ml is diluted by a factor of .00125 so 10N x .00125 = .0125 N final concentration. > If using a 1 N stock solution, just add 10X the volume or 12.5 ml 1 N stock to 987.5 ml water. > > Hope this helps, > > Tresa > > -----Original Message----- > From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Bernice Frederick > Sent: Thursday, April 30, 2015 1:35 PM > To: Histonet at lists.utsouthwestern.edu > Subject: [Histonet] Question > > All, > I have a procedure here that call for and I quote "1.25 ml NaOH 10N in 1L of water." I know how to get 1 N, but how do I get 10. Having rarely hd the opportunity to make many Normal solutions ,my brain is not computing. Is it an error? > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick at northwestern.edu > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff at rwjms.rutgers.edu ********************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV at archildrens.org Fri May 1 10:49:43 2015 From: HornHV at archildrens.org (Horn, Hazel V) Date: Fri, 1 May 2015 10:49:43 -0500 Subject: [Histonet] IHC billing question In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> Message-ID: <25A4DE08332B19499904459F00AAACB71A1EB25529@EVS1.archildrens.org> We have 2 billing codes for IHC. One for the first IHC and another for all additional IHC's on the same case. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] Sent: Friday, May 01, 2015 8:13 AM To: 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing question I do it every day - change every first to 88342. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, April 30, 2015 5:33 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC billing question Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Joyce.Weems at emoryhealthcare.org Fri May 1 10:51:27 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Fri, 1 May 2015 15:51:27 +0000 Subject: [Histonet] IHC billing question In-Reply-To: <25A4DE08332B19499904459F00AAACB71A1EB25529@EVS1.archildrens.org> References: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> <25A4DE08332B19499904459F00AAACB71A1EB25529@EVS1.archildrens.org> Message-ID: And what LIS to you have? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Horn, Hazel V [mailto:HornHV at archildrens.org] Sent: Friday, May 01, 2015 11:50 AM To: Weems, Joyce K.; 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: RE: IHC billing question We have 2 billing codes for IHC. One for the first IHC and another for all additional IHC's on the same case. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] Sent: Friday, May 01, 2015 8:13 AM To: 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing question I do it every day - change every first to 88342. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, April 30, 2015 5:33 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC billing question Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From HornHV at archildrens.org Fri May 1 10:59:57 2015 From: HornHV at archildrens.org (Horn, Hazel V) Date: Fri, 1 May 2015 10:59:57 -0500 Subject: [Histonet] IHC billing question In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> <25A4DE08332B19499904459F00AAACB71A1EB25529@EVS1.archildrens.org> Message-ID: <25A4DE08332B19499904459F00AAACB71A1EB2552A@EVS1.archildrens.org> Meditech Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] Sent: Friday, May 01, 2015 10:51 AM To: Horn, Hazel V; 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: RE: IHC billing question And what LIS to you have? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Horn, Hazel V [mailto:HornHV at archildrens.org] Sent: Friday, May 01, 2015 11:50 AM To: Weems, Joyce K.; 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: RE: IHC billing question We have 2 billing codes for IHC. One for the first IHC and another for all additional IHC's on the same case. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] Sent: Friday, May 01, 2015 8:13 AM To: 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing question I do it every day - change every first to 88342. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, April 30, 2015 5:33 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC billing question Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Joyce.Weems at emoryhealthcare.org Fri May 1 11:02:41 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Fri, 1 May 2015 16:02:41 +0000 Subject: [Histonet] IHC billing question In-Reply-To: <25A4DE08332B19499904459F00AAACB71A1EB2552A@EVS1.archildrens.org> References: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> <25A4DE08332B19499904459F00AAACB71A1EB25529@EVS1.archildrens.org> <25A4DE08332B19499904459F00AAACB71A1EB2552A@EVS1.archildrens.org> Message-ID: We have CoPath. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Horn, Hazel V [mailto:HornHV at archildrens.org] Sent: Friday, May 01, 2015 12:00 PM To: Weems, Joyce K.; 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: RE: IHC billing question Meditech Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] Sent: Friday, May 01, 2015 10:51 AM To: Horn, Hazel V; 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: RE: IHC billing question And what LIS to you have? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Horn, Hazel V [mailto:HornHV at archildrens.org] Sent: Friday, May 01, 2015 11:50 AM To: Weems, Joyce K.; 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: RE: IHC billing question We have 2 billing codes for IHC. One for the first IHC and another for all additional IHC's on the same case. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] Sent: Friday, May 01, 2015 8:13 AM To: 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing question I do it every day - change every first to 88342. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, April 30, 2015 5:33 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC billing question Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From jvickroy at SpringfieldClinic.com Fri May 1 11:15:35 2015 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Fri, 1 May 2015 16:15:35 +0000 Subject: [Histonet] Combination bracket for Gross lab senior Message-ID: <9B1A1501A800064397369BD8072E6BCAB67E44@E2K10DB.springfieldclinic.com> Does anybody have an idea how to attach the combination bracket with a shelf for the Printmate from Thermo Fisher. No instructions and trying to conceptualize how this thing goes together. Worse than putting together a swing set or new kid's bicycle. Diagram or picture with one attached would be helpful. Manufacturer trying to find some instructions also. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From gayle.callis at bresnan.net Fri May 1 11:50:52 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Fri, 1 May 2015 10:50:52 -0600 Subject: [Histonet] long answer RE: GMS Question Message-ID: <001601d0842e$fc45f6b0$f4d1e410$@bresnan.net> Hi Paula, The same site of infection for your control and patient tissues does not mean the fungus species was the same. Now for addtional commentary. The classic GMS uses chromic acid as the oxidizer, stronger than periodic acid. This is something to beware of since you had a false negative result with the kit. Periodic acid should be freshly made up from periodic acid crystals just before use (Culling insisted on fresh periodic acid reagent) then discarded after that day - something that is not going on with a kit where all components are sent to you in ready to use liquid form. I think if you had used the classic GMS with chromic acid to start with, you would have had the results seen with Periodic Acid/Schiffs. Part of the problem is the not all fungus species stain well either PAS or Periodic acid/GMS, and even classic GMS. All these factors can present a problem when trying to diagnose fungal infections. Lee Luna in AFIP manual, pointed out "it has been found that the time of exposure to methenamine-silver nitrate solution for complete development may vary according to type and/or strains suspected." He advised if Nocardia asteroides is suspected, then two slides should be run: one for 60 minutes and one for 90 min in the silver solution. Histotechs should be aware these possible problems. Fungi are difficult at best even when doing fungus cultures and treat. Fortunately, there are antibodies for some fungi i.e. Aspergillus, Candida) but you would probably need the specific fungus as a control for the antibody being used. Fungus IHC experts can weigh in on the latter topic. I don't know what fungus species was in your "fungus ball" control block, but our clinical lab fungus ball control contained Aspergillus sp. from a post mortem human lung. With the researcher, he only had pure Aspergillus sp. infecting the tissues. It would be nice to know what species of fungus is in your control tissue block???? The publication by Frieda Carson along with Jerry Fredenburgh and John Maxwell "Inconsistent detection of Histoplasma capsulatum with periodic acid in GMS fungus stain" , J Histotechnology, 1999 is a profound statement about what you just experienced. Their tissue control i.e. Aspergillus sp. stained adequately with periodic acid/GMS but the H. capsulatum fungus in tissue did not stain. They studied several different times, temperatures, periodic acid concentrations and fungus species. They had additional controls containing fungi other than Aspergillus sp. for better sampling of PA/GMS on different fungi. Having different fungus species control blocks is a luxury many labs do not enjoy. The reason chromic acid is not in kits is due to shipping, health and safety hazards, must be handled carefully and collected for proper, safe disposal so it is easier to make up "GMS" kits with periodic acid. If you have to collect your silver solutions for safe chemical disposal, then chromic acid shouldn't be a big problem to do the same. Also, since Periodic acid/Schiffs is popular and commonly used for fungus staining, PAS can also present false negative results or weak staining due to the weaker periodic acid oxidation, even when the periodic acid is made in house, fresh for the day. Chromic acid/Schiffs has been recommended by people on Histonet to improve fungus staining over PAS. PAS stain always seem to work well with Candida sp. and Aspergillus sp. but classic GMS was always better in my hands. I only used classic GMS, prepared in house, and controlled the development with a microscope to avoid under and over silver staining of fungus. I will be happy to send the Carson et al publication and scan the AFIP manual pages with photos on fungus staining by Luna. I apologize for long discourse as chromic acid/GMS stain is one of my favorite special stains to perform along with a long standing interest in fungus staining. I hope this helps. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: Paula Lucas [mailto:plucas at biopath.org] Sent: Friday, May 01, 2015 8:17 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] GMS Question Hello, I think I already know the answer but I'm not sure why so if someone can help me understand the theory behind it, I would greatly appreciate it. Currently, we use the Richard Allen kit for the GMS stain and it uses Periodic Acid as the 1st step. We use a control tissue from a case we had that was positive for fungus and it's a fungus ball from the Rt Maxillary. We ran a test for fungus on a different and current case of the same tissue (different patient): Rt Maxillary sinus. The control tissue did work, but the patient's tissue did not, so the doctor ordered a PAS for fungus and this clearly showed the fungal elements nicely. My question is why would the control and patient tissue have different results when they are both fungus balls from the same specimen source? Thanks in advance, Paula Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike at pathview.com Fri May 1 12:06:06 2015 From: mike at pathview.com (Michael Mihalik) Date: Fri, 1 May 2015 10:06:06 -0700 Subject: [Histonet] IHC billing question In-Reply-To: <25A4DE08332B19499904459F00AAACB71A1EB25529@EVS1.archildrens.org> References: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> <25A4DE08332B19499904459F00AAACB71A1EB25529@EVS1.archildrens.org> Message-ID: <00b201d08431$1dd819f0$59884dd0$@pathview.com> Am I misunderstanding, or should it be per specimen and not per case? Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Horn, Hazel V [mailto:HornHV at archildrens.org] Sent: Friday, May 01, 2015 8:50 AM To: 'Weems, Joyce K.'; 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing question We have 2 billing codes for IHC. One for the first IHC and another for all additional IHC's on the same case. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] Sent: Friday, May 01, 2015 8:13 AM To: 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing question I do it every day - change every first to 88342. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, April 30, 2015 5:33 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC billing question Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins at mt.gov Fri May 1 12:28:54 2015 From: TGoins at mt.gov (Goins, Tresa) Date: Fri, 1 May 2015 17:28:54 +0000 Subject: [Histonet] GMS Question In-Reply-To: References: Message-ID: To get a positive PAS or GMS fungal stain, one must oxidize the carbohydrate in the fungal cell wall. Chromic acid is a stronger oxidizer than periodic acid, so would work better with mature fungal cell walls that are highly polymerized. Treat an immature cell wall for too long, and you may get a false negative because the carbohydrate structure no longer resembles a fungal cell wall. Tresa -----Original Message----- From: Paula Lucas [mailto:plucas at biopath.org] Sent: Friday, May 01, 2015 8:17 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] GMS Question Hello, I think I already know the answer but I'm not sure why so if someone can help me understand the theory behind it, I would greatly appreciate it. Currently, we use the Richard Allen kit for the GMS stain and it uses Periodic Acid as the 1st step. We use a control tissue from a case we had that was positive for fungus and it's a fungus ball from the Rt Maxillary. We ran a test for fungus on a different and current case of the same tissue (different patient): Rt Maxillary sinus. The control tissue did work, but the patient's tissue did not, so the doctor ordered a PAS for fungus and this clearly showed the fungal elements nicely. My question is why would the control and patient tissue have different results when they are both fungus balls from the same specimen source? Thanks in advance, Paula Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver at hotmail.com Fri May 1 13:03:39 2015 From: joelleweaver at hotmail.com (Joelle Weaver) Date: Fri, 1 May 2015 18:03:39 +0000 Subject: [Histonet] IHC billing question In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org>, , , Message-ID: Histologists enter all TC IHC billing codes manually as performed before they leave the lab. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: garreyf at gmail.com > Date: Thu, 30 Apr 2015 17:52:25 -0400 > To: mpence at grhs.net > Subject: Re: [Histonet] IHC billing question > CC: histonet at lists.utsouthwestern.edu; Richard.Cartun at hhchealth.org > > Your Lis should not have done that. > If you are using Copath/Cerner, I think they have automated it now according to their most recent newsletter. Currently, I have to manually change the 88342's to 1's It's somewhat of a pain. But, your Lis should have consulted someone before deleting all codes. Your pathologists should have been on top of it as well imho. Unfortunately, someone has to change. It may be too late to bill too for some. > Garrey > > Sent from my iPhone > > > On Apr 30, 2015, at 5:44 PM, Mike Pence wrote: > > > > We do all of our IHC billing manually. > > > > -----Original Message----- > > From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > > Sent: Thursday, April 30, 2015 4:43 PM > > To: Cartun, Richard > > Cc: histonet at lists.utsouthwestern.edu > > Subject: Re: [Histonet] IHC billing question > > > > We have to manually review the IHC billing also and continue to audit. It took billing and IT three months to create the logic to automate billing for a specimen and account for combination of there being the possibility of 88341, 88342 & 88344 and the proper combinations. > > > > Sent from my iPhone > > > >> On Apr 30, 2015, at 2:34 PM, Cartun, Richard wrote: > >> > >> Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). > >> > >> Richard > >> > >> Richard W. Cartun, MS, PhD > >> Director, Histology & Immunopathology > >> Director, Biospecimen Collection Programs Assistant Director, Anatomic > >> Pathology Hartford Hospital > >> 80 Seymour Street > >> Hartford, CT 06102 > >> (860) 972-1596 > >> (860) 545-2204 Fax > >> > >> > >> This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems at emoryhealthcare.org Fri May 1 13:21:41 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Fri, 1 May 2015 18:21:41 +0000 Subject: [Histonet] IHC billing question In-Reply-To: <00b201d08431$1dd819f0$59884dd0$@pathview.com> References: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> <25A4DE08332B19499904459F00AAACB71A1EB25529@EVS1.archildrens.org> <00b201d08431$1dd819f0$59884dd0$@pathview.com> Message-ID: It's per specimen - 88342 for the first ab on a specimen, 88341 for ea additional on same specimen. (Not multiple blocks on same specimen). Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Michael Mihalik [mailto:mike at pathview.com] Sent: Friday, May 01, 2015 1:06 PM To: 'Horn, Hazel V'; Weems, Joyce K.; 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] IHC billing question Am I misunderstanding, or should it be per specimen and not per case? Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Horn, Hazel V [mailto:HornHV at archildrens.org] Sent: Friday, May 01, 2015 8:50 AM To: 'Weems, Joyce K.'; 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing question We have 2 billing codes for IHC. One for the first IHC and another for all additional IHC's on the same case. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] Sent: Friday, May 01, 2015 8:13 AM To: 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing question I do it every day - change every first to 88342. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, April 30, 2015 5:33 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC billing question Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Fri May 1 13:34:25 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 1 May 2015 18:34:25 +0000 Subject: [Histonet] IHC billing CoPath workaround In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F8DDDF@HRHEX02-HOS.holyredeemer.local> We have a great workaround in built in CoPath. Every antibody in our IHC dictionary, is built in triplicate. One is called "[Ab name]-Primary" with a 88342 CPT code, the second is called "[Ab name]-Add" with an 88341 CPT code. The third is "[Ab name]-NC for no charge or N/A in the CoPath dictionary. This is for when the pathologists wants the same antibody on multiple blocks for the same specimen (which can't be charged) Then we just educated everyone on how to order correctly and provided a cheat sheet. For panels, the stain groups were built correctly so that the first Ab ordered is always 88342, and the remaining charge as 88341. It has worked very well for us with few mistakes at implementation. It bills correctly, counts stained slides correctly, and orders across the interface to the stainer correctly, so that we get the right bar code labels for our IHC stainer. A win-win situation. From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, April 30, 2015 5:33 PM Subject: [Histonet] IHC billing question Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax ************************** From Joyce.Weems at emoryhealthcare.org Fri May 1 13:39:31 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Fri, 1 May 2015 18:39:31 +0000 Subject: [Histonet] IHC billing CoPath workaround In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8DDDF@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8DDDF@HRHEX02-HOS.holyredeemer.local> Message-ID: Interesting! Thanks! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Terri Braud [mailto:tbraud at holyredeemer.com] Sent: Friday, May 01, 2015 2:34 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing CoPath workaround We have a great workaround in built in CoPath. Every antibody in our IHC dictionary, is built in triplicate. One is called "[Ab name]-Primary" with a 88342 CPT code, the second is called "[Ab name]-Add" with an 88341 CPT code. The third is "[Ab name]-NC for no charge or N/A in the CoPath dictionary. This is for when the pathologists wants the same antibody on multiple blocks for the same specimen (which can't be charged) Then we just educated everyone on how to order correctly and provided a cheat sheet. For panels, the stain groups were built correctly so that the first Ab ordered is always 88342, and the remaining charge as 88341. It has worked very well for us with few mistakes at implementation. It bills correctly, counts stained slides correctly, and orders across the interface to the stainer correctly, so that we get the right bar code labels for our IHC stainer. A win-win situation. From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, April 30, 2015 5:33 PM Subject: [Histonet] IHC billing question Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax ************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From gayle.callis at bresnan.net Fri May 1 14:17:25 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Fri, 1 May 2015 13:17:25 -0600 Subject: [Histonet] GMS Question In-Reply-To: References: Message-ID: <000d01d08443$75d47ec0$617d7c40$@bresnan.net> Hi Tresa, What staining parameters do you suggest for seeing mature and/or immature fungal cell walls? I don't think you will know what level of maturity is present before doing a fungus stain. But wouldn't both levels of maturity both be present if the fungus is actively growing in a tissue? What do you recommend, using both a PAS, or chromic acid/Schiffs along with chromic acid GMS method? We used 4% chromic acid at RT for 1 hour but would you recommend duplicating slides so you do pull one slide out of chromic acid at 30 minutes and one for 60 minutes to stain either mature or immature fungal and/or both cell wall structure. Gayle Callis HTL/HT/MT (ASCP) -----Original Message----- From: Goins, Tresa [mailto:TGoins at mt.gov] Sent: Friday, May 01, 2015 11:29 AM To: Paula Lucas; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] GMS Question To get a positive PAS or GMS fungal stain, one must oxidize the carbohydrate in the fungal cell wall. Chromic acid is a stronger oxidizer than periodic acid, so would work better with mature fungal cell walls that are highly polymerized. Treat an immature cell wall for too long, and you may get a false negative because the carbohydrate structure no longer resembles a fungal cell wall. Tresa -----Original Message----- From: Paula Lucas [mailto:plucas at biopath.org] Sent: Friday, May 01, 2015 8:17 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] GMS Question Hello, I think I already know the answer but I'm not sure why so if someone can help me understand the theory behind it, I would greatly appreciate it. Currently, we use the Richard Allen kit for the GMS stain and it uses Periodic Acid as the 1st step. We use a control tissue from a case we had that was positive for fungus and it's a fungus ball from the Rt Maxillary. We ran a test for fungus on a different and current case of the same tissue (different patient): Rt Maxillary sinus. The control tissue did work, but the patient's tissue did not, so the doctor ordered a PAS for fungus and this clearly showed the fungal elements nicely. My question is why would the control and patient tissue have different results when they are both fungus balls from the same specimen source? Thanks in advance, Paula Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abtdhu at gmail.com Fri May 1 14:42:54 2015 From: abtdhu at gmail.com (Dorothy Hu) Date: Fri, 1 May 2015 15:42:54 -0400 Subject: [Histonet] Bubble in MMA blocks Message-ID: Hi histonetter and specifically Gayle Callis, I try to explain why we need to leave MMA embedded glass vials in water bath during embedding to a research lab. But I need experts to have a convencing explaination. The lab use vacuum desiccator (which has sealed ring to keep vacuum) to leave the vials from 4 degree overnight, remove it to room temperature for 3 hours, then move to 32 degree oven with the vials in the vacuum dessiccator. They often found there are bubbles in the vial after polymerization down. I knew they have change the way to do embedding by remove the vial from dessiccator to close the cap and leave the vial in water bath and space out to vent the heat generated during polymerization. But how can I say the vacuum dessiccator. Thanks everyone to explain. Dorothy Hu From TGoins at mt.gov Fri May 1 14:55:29 2015 From: TGoins at mt.gov (Goins, Tresa) Date: Fri, 1 May 2015 19:55:29 +0000 Subject: [Histonet] GMS Question In-Reply-To: <000d01d08443$75d47ec0$617d7c40$@bresnan.net> References: <000d01d08443$75d47ec0$617d7c40$@bresnan.net> Message-ID: Hi Gayle - We have never had a "noticeable" problem detecting fungi using 5% aqueous chromic acid at room temperature for 1 hr. The presence of mature and immature wall structures may be the reason. We have never tried to determine the "end point" for over oxidation, but my best guess is that it is much longer than 60 minutes. More often, I think a false negative would result from inadequate oxidation of the fungal cell wall. In addition to cell wall maturity, I believe the composition adds to differences in rates of oxidation, the major contributors being the prevalence of precursors susceptible to aldehyde formation and melanin. One needs enough product to produce a visible color reaction. A complex question and any more detail and I will have to hit the books. Tresa -----Original Message----- From: Gayle Callis [mailto:gayle.callis at bresnan.net] Sent: Friday, May 01, 2015 1:17 PM To: Goins, Tresa; histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] GMS Question Hi Tresa, What staining parameters do you suggest for seeing mature and/or immature fungal cell walls? I don't think you will know what level of maturity is present before doing a fungus stain. But wouldn't both levels of maturity both be present if the fungus is actively growing in a tissue? What do you recommend, using both a PAS, or chromic acid/Schiffs along with chromic acid GMS method? We used 4% chromic acid at RT for 1 hour but would you recommend duplicating slides so you do pull one slide out of chromic acid at 30 minutes and one for 60 minutes to stain either mature or immature fungal and/or both cell wall structure. Gayle Callis HTL/HT/MT (ASCP) -----Original Message----- From: Goins, Tresa [mailto:TGoins at mt.gov] Sent: Friday, May 01, 2015 11:29 AM To: Paula Lucas; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] GMS Question To get a positive PAS or GMS fungal stain, one must oxidize the carbohydrate in the fungal cell wall. Chromic acid is a stronger oxidizer than periodic acid, so would work better with mature fungal cell walls that are highly polymerized. Treat an immature cell wall for too long, and you may get a false negative because the carbohydrate structure no longer resembles a fungal cell wall. Tresa -----Original Message----- From: Paula Lucas [mailto:plucas at biopath.org] Sent: Friday, May 01, 2015 8:17 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] GMS Question Hello, I think I already know the answer but I'm not sure why so if someone can help me understand the theory behind it, I would greatly appreciate it. Currently, we use the Richard Allen kit for the GMS stain and it uses Periodic Acid as the 1st step. We use a control tissue from a case we had that was positive for fungus and it's a fungus ball from the Rt Maxillary. We ran a test for fungus on a different and current case of the same tissue (different patient): Rt Maxillary sinus. The control tissue did work, but the patient's tissue did not, so the doctor ordered a PAS for fungus and this clearly showed the fungal elements nicely. My question is why would the control and patient tissue have different results when they are both fungus balls from the same specimen source? Thanks in advance, Paula Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From craigak12 at gmail.com Fri May 1 15:10:19 2015 From: craigak12 at gmail.com (Jb) Date: Fri, 1 May 2015 13:10:19 -0700 Subject: [Histonet] Tissue transfer method: Message-ID: Does anyone have a procedure that they can share on tissue transfer? One stained slide to another? Thank you for your help. Sincerely, Craig Sent from my iPhone From tony.henwood at health.nsw.gov.au Fri May 1 15:10:18 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 1 May 2015 20:10:18 +0000 Subject: [Histonet] IHC and oven temperature In-Reply-To: <1835135147.1524546.1430423762046.JavaMail.yahoo@mail.yahoo.com> References: <6D6BD1DE8A5571489398B392A38A7157F53EBEE3@xmdb04.nch.kids>, <1835135147.1524546.1430423762046.JavaMail.yahoo@mail.yahoo.com> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53EC4C4@xmdb04.nch.kids> Hi Rene, Contradiction? Possibly but HIER is done in an aqueous environment, whereas the oven heating above 60oC was done dry (I will send you a copy of the article under a separate email). Regards, Tony (from down under, currently in London UK). ________________________________________ From: Rene J Buesa [rjbuesa at yahoo.com] Sent: Friday, 1 May 2015 5:56 AM To: Tony Henwood (SCHN); Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC and oven temperature What about HIER at 95-98?C? "Everybody" uses it to "enhance" epitopes detection. Is there not an intrinsic contradiction here? Ren? J. On Thursday, April 30, 2015 3:32 PM, Tony Henwood (SCHN) wrote: Yes, I read the Dako IPX educational guides (5th ed) and on page 32: "No processes should raise tissue temperature to higher than 60oC as this will cause severe loss of antigenicity that may not be recoverable" Unfortunately there is no evidence given or cited that validates this statement. Even though this could be right (and there are several papers that have looked at this), this statement is scientifically weak and we should not cite this as truth. Now I do recommend the Dako reference series to my students, and I have contributed to one of these texts myself (Microscopic control of routine H&E - know your histology) but I request my students to continue to question what they read and confirm the scientific validity of the information. Regards, Tony ________________________________________ From: Joelle Weaver [joelleweaver at hotmail.com] Sent: Saturday, 25 April 2015 5:51 AM To: Tony Henwood (SCHN); WILLIAM DESALVO; Preiszner, Johanna Cc: histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] IHC and oven temperature I remember reading that the preffered temperature was about 60 degrees Celsius. I think that this was in the Dako education guides if I'm not mistaken. If that is the case, the citation for the source is probably in that resource available as pdf from their website. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tony.henwood at health.nsw.gov.au > To: wdesalvo.cac at outlook.com; PREISZNE at mail.etsu.edu > Date: Fri, 24 Apr 2015 09:43:59 +0000 > Subject: RE: [Histonet] IHC and oven temperature > CC: histonet at lists.utsouthwestern.edu > > Hi temp drying shown to be a bad idea: > > Henwood, A., (2005) ?Effect of Slide Drying at 80?C on Immunohistochemistry? J Histotechnol 28(1):45-46. > > Abstract > > Prolonged high temperature dry heating has been found to be deleterious to the immunohistochemical demonstration of several antigens in formalin-fixed, paraffin- embedded sections. Paraffin sections were dried at 80?C for 7 h and their immunoreactivity was compared with mirror sections dried for 1 h at 60?C. NCL-5D3, CMV, S100, HMB45, and CEA were quite labile to dry overheating whereas AElAE3, HBsAg, HBcAg, HSVII, EMA, chromogranin, and NSE were found to be quite resistant. It is recommended that coated slides (poly-L-lysine or aminopropyltriethoxysilane) and low-temperature drying (<60?C) be routinely used for irnmunohistochemistry. > > ________________________________________ > From: histonet-bounces at lists.utsouthwestern.edu [histonet-bounces at lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO [wdesalvo.cac at outlook.com] > Sent: Tuesday, 21 April 2015 1:56 AM > To: Preiszner, Johanna > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC and oven temperature > > Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes > > Sent from my iPhone > > > On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna > wrote: > > > > Hi Netters, > > > > is there something wrong with this logic: > > > > "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven." > > > > Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer... > > > > Thanks! > > > > Hanna Preiszner > > ETSU/QCOM > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet On Thursday, April 30, 2015 3:32 PM, Tony Henwood (SCHN) wrote: Yes, I read the Dako IPX educational guides (5th ed) and on page 32: "No processes should raise tissue temperature to higher than 60oC as this will cause severe loss of antigenicity that may not be recoverable" Unfortunately there is no evidence given or cited that validates this statement. Even though this could be right (and there are several papers that have looked at this), this statement is scientifically weak and we should not cite this as truth. Now I do recommend the Dako reference series to my students, and I have contributed to one of these texts myself (Microscopic control of routine H&E - know your histology) but I request my students to continue to question what they read and confirm the scientific validity of the information. Regards, Tony ________________________________________ From: Joelle Weaver [joelleweaver at hotmail.com] Sent: Saturday, 25 April 2015 5:51 AM To: Tony Henwood (SCHN); WILLIAM DESALVO; Preiszner, Johanna Cc: histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] IHC and oven temperature I remember reading that the preffered temperature was about 60 degrees Celsius. I think that this was in the Dako education guides if I'm not mistaken. If that is the case, the citation for the source is probably in that resource available as pdf from their website. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tony.henwood at health.nsw.gov.au > To: wdesalvo.cac at outlook.com; PREISZNE at mail.etsu.edu > Date: Fri, 24 Apr 2015 09:43:59 +0000 > Subject: RE: [Histonet] IHC and oven temperature > CC: histonet at lists.utsouthwestern.edu > > Hi temp drying shown to be a bad idea: > > Henwood, A., (2005) ?Effect of Slide Drying at 80?C on Immunohistochemistry? J Histotechnol 28(1):45-46. > > Abstract > > Prolonged high temperature dry heating has been found to be deleterious to the immunohistochemical demonstration of several antigens in formalin-fixed, paraffin- embedded sections. Paraffin sections were dried at 80?C for 7 h and their immunoreactivity was compared with mirror sections dried for 1 h at 60?C. NCL-5D3, CMV, S100, HMB45, and CEA were quite labile to dry overheating whereas AElAE3, HBsAg, HBcAg, HSVII, EMA, chromogranin, and NSE were found to be quite resistant. It is recommended that coated slides (poly-L-lysine or aminopropyltriethoxysilane) and low-temperature drying (<60?C) be routinely used for irnmunohistochemistry. > > ________________________________________ > From: histonet-bounces at lists.utsouthwestern.edu [histonet-bounces at lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO [wdesalvo.cac at outlook.com] > Sent: Tuesday, 21 April 2015 1:56 AM > To: Preiszner, Johanna > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC and oven temperature > > Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes > > Sent from my iPhone > > > On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna > wrote: > > > > Hi Netters, > > > > is there something wrong with this logic: > > > > "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven." > > > > Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer... > > > > Thanks! > > > > Hanna Preiszner > > ETSU/QCOM > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From JWatson at gnf.org Fri May 1 15:21:05 2015 From: JWatson at gnf.org (James Watson) Date: Fri, 1 May 2015 20:21:05 +0000 Subject: [Histonet] Tissue transfer method: In-Reply-To: References: Message-ID: Slide Repair 1. Soak coverslip off of slide in xylene. 2. Piece slide back together. 3. Apply Mount-Quick to slide, covering tissue. Allow to dry overnight. 4. Place slide in cold water in refrigerator overnight. 5. Carefully peal the Mount-Quick off the slide. The tissue should come up with the Mount-Quick, if it does not stop pealing up the Mount-Quick and place back into the water overnight again. 6. Carefully trim excess Mount-Quick from areas where tissue is not present. Leave at least ? inch of Mount-Quick around the tissue 7. On a new slide that is wet with water, place the removed Mount-Quick and tissue in the center of the slide (make sure the tissue is next to the slide), removing as many air bubbles from under the Mount-Quick as possible. 8. Cover the slide with a wet piece of filter paper then place a blank slide on top. If you have multiple slides to repair then they can be stacked. Apply weight to the top of this and let stand overnight. 9. Soak the dry slide in Xylene until the Mount-Quick is removed and coverslip as usual. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson at gnf.org -----Original Message----- From: Jb [mailto:craigak12 at gmail.com] Sent: Friday, May 01, 2015 1:10 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Tissue transfer method: Does anyone have a procedure that they can share on tissue transfer? One stained slide to another? Thank you for your help. Sincerely, Craig Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA at mercer.edu Fri May 1 15:27:10 2015 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Fri, 1 May 2015 16:27:10 -0400 Subject: [Histonet] Tissue transfer method: In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE25C0298ACF7@MERCERMAIL.MercerU.local> With Mount Quick you can also decolorize the section and do a different stain on them. I have even performed IHC on these transferred sections when needed. Shirley Powell -----Original Message----- From: James Watson [mailto:JWatson at gnf.org] Sent: Friday, May 01, 2015 4:21 PM To: 'Jb'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue transfer method: Slide Repair 1. Soak coverslip off of slide in xylene. 2. Piece slide back together. 3. Apply Mount-Quick to slide, covering tissue. Allow to dry overnight. 4. Place slide in cold water in refrigerator overnight. 5. Carefully peal the Mount-Quick off the slide. The tissue should come up with the Mount-Quick, if it does not stop pealing up the Mount-Quick and place back into the water overnight again. 6. Carefully trim excess Mount-Quick from areas where tissue is not present. Leave at least ? inch of Mount-Quick around the tissue 7. On a new slide that is wet with water, place the removed Mount-Quick and tissue in the center of the slide (make sure the tissue is next to the slide), removing as many air bubbles from under the Mount-Quick as possible. 8. Cover the slide with a wet piece of filter paper then place a blank slide on top. If you have multiple slides to repair then they can be stacked. Apply weight to the top of this and let stand overnight. 9. Soak the dry slide in Xylene until the Mount-Quick is removed and coverslip as usual. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson at gnf.org -----Original Message----- From: Jb [mailto:craigak12 at gmail.com] Sent: Friday, May 01, 2015 1:10 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Tissue transfer method: Does anyone have a procedure that they can share on tissue transfer? One stained slide to another? Thank you for your help. Sincerely, Craig Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Fri May 1 15:27:39 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 1 May 2015 20:27:39 +0000 Subject: [Histonet] IHC and oven temperature In-Reply-To: <43D1B55674EC2244A707ACE779624362DC4E76@DHRNASVXM06.danaher.org> References: ,, , <6D6BD1DE8A5571489398B392A38A7157F53EAC93@xmdb04.nch.kids>, , <6D6BD1DE8A5571489398B392A38A7157F53EBED0@xmdb04.nch.kids> <6D6BD1DE8A5571489398B392A38A7157F53EBEE3@xmdb04.nch.kids>, <43D1B55674EC2244A707ACE779624362DC4E76@DHRNASVXM06.danaher.org> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53EC514@xmdb04.nch.kids> Hi Charles, "In this study, having previously heated the tissue to 80 degrees would improve and speed up antibody-antigen reaction by ensuring the antigen was denatured." But not all antibodies, some were not affected, others were adversely affected. The cell and cell molecules are complex and variable. I will send you a copy of the paper in a separate email Regards Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Scouten, Charles [Charles.Scouten at LeicaBiosystems.com] Sent: Friday, 1 May 2015 7:51 AM To: Tony Henwood (SCHN); Histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] IHC and oven temperature I have recently reviewed literature about sacrifice microwave fixation. Proteins in the living brain are denatured in as little as .3 seconds, stopping all brain chemical reactions (all catalyzed by enzymes to tightly regulate brain), freezing the chemical state of the brain. Proteins, including enzymes, in the mammalian body denature between about 40 degrees C to 80 degrees C. Depending on the specific protein, each of which would have a denature temperature. In formation, proteins fold, and then fold again. Enzyme active sites are in the tertiary structure. Proteins can be denatured, unfolded, destroying enzymes, without losing antigenicity, depending on what the antibody is attaching to. Heating up to 90 degrees or higher does not break apart the proteins amino acid chain, which has much stronger bonds than the hydrogen bonds that hold the protein folded. My guess about the statement below is that it oversimplifies. Depending on which antigen you are going for, what its own denature temperature is, and whether the antibody in question reaches across folds to bind (is this known in any case?) you may or may not lose antigenicity between 40 degrees C and 80 degrees C. Some monoclonal antibodies raised with a native protein bind preferentially to the denatured antigen Bertrand Friguet, Lisa Djavadi-Ohaniance, Michel E. Goldberg In this study, having previously heated the tissue to 80 degrees would improve and speed up antibody-antigen reaction by ensuring the antigen was denatured. Cordially, Charles W. Scouten, Ph.D. Applications Specialist Leica Biosystems Charles.Scouten at Leicabiosystems.com http://www.myneurolab.com Ph. 630 964 0501 Cell 314 724 5920 -----Original Message----- From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Tony Henwood (SCHN) Sent: Thursday, April 30, 2015 2:32 PM To: Histonet at lists.utsouthwestern.edu Subject: FW: [Histonet] IHC and oven temperature Yes, I read the Dako IPX educational guides (5th ed) and on page 32: "No processes should raise tissue temperature to higher than 60oC as this will cause severe loss of antigenicity that may not be recoverable" Unfortunately there is no evidence given or cited that validates this statement. Even though this could be right (and there are several papers that have looked at this), this statement is scientifically weak and we should not cite this as truth. Now I do recommend the Dako reference series to my students, and I have contributed to one of these texts myself (Microscopic control of routine H&E - know your histology) but I request my students to continue to question what they read and confirm the scientific validity of the information. Regards, Tony ________________________________________ From: Joelle Weaver [joelleweaver at hotmail.com] Sent: Saturday, 25 April 2015 5:51 AM To: Tony Henwood (SCHN); WILLIAM DESALVO; Preiszner, Johanna Cc: histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] IHC and oven temperature I remember reading that the preffered temperature was about 60 degrees Celsius. I think that this was in the Dako education guides if I'm not mistaken. If that is the case, the citation for the source is probably in that resource available as pdf from their website. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tony.henwood at health.nsw.gov.au > To: wdesalvo.cac at outlook.com; PREISZNE at mail.etsu.edu > Date: Fri, 24 Apr 2015 09:43:59 +0000 > Subject: RE: [Histonet] IHC and oven temperature > CC: histonet at lists.utsouthwestern.edu > > Hi temp drying shown to be a bad idea: > > Henwood, A., (2005) "Effect of Slide Drying at 80?C on Immunohistochemistry" J Histotechnol 28(1):45-46. > > Abstract > > Prolonged high temperature dry heating has been found to be deleterious to the immunohistochemical demonstration of several antigens in formalin-fixed, paraffin- embedded sections. Paraffin sections were dried at 80?C for 7 h and their immunoreactivity was compared with mirror sections dried for 1 h at 60?C. NCL-5D3, CMV, S100, HMB45, and CEA were quite labile to dry overheating whereas AElAE3, HBsAg, HBcAg, HSVII, EMA, chromogranin, and NSE were found to be quite resistant. It is recommended that coated slides (poly-L-lysine or aminopropyltriethoxysilane) and low-temperature drying (<60?C) be routinely used for irnmunohistochemistry. > > ________________________________________ > From: histonet-bounces at lists.utsouthwestern.edu > [histonet-bounces at lists.utsouthwestern.edu] on behalf of WILLIAM > DESALVO [wdesalvo.cac at outlook.com] > Sent: Tuesday, 21 April 2015 1:56 AM > To: Preiszner, Johanna > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC and oven temperature > > Dry heat compared to wet heat. Do not "dry" your slides at high heat. > You are removing water trapped between slide and paraffin section. > Antigen retrieval is an entirely different process. So not try to > combine the two processes > > Sent from my iPhone > > > On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna wrote: > > > > Hi Netters, > > > > is there something wrong with this logic: > > > > "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven." > > > > Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer... > > > > Thanks! > > > > Hanna Preiszner > > ETSU/QCOM > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************** > *********** This email and any files transmitted with it are > confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > *********** > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Please be advised that this email may contain confidential information. If you are not the intended recipient, please notify us by email by replying to the sender and delete this message. The sender disclaims that the content of this email constitutes an offer to enter into, or the acceptance of, any agreement; provided that the foregoing does not invalidate the binding effect of any digital or other electronic reproduction of a manual signature that is included in any attachment. ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Bonnie.Whitaker at osumc.edu Fri May 1 15:32:45 2015 From: Bonnie.Whitaker at osumc.edu (Whitaker, Bonnie) Date: Fri, 1 May 2015 16:32:45 -0400 Subject: [Histonet] Tissue transfer method: In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25C0298ACF7@MERCERMAIL.MercerU.local> References: <9BF995BC0E47744E9673A41486E24EE25C0298ACF7@MERCERMAIL.MercerU.local> Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670E160223A4@EXMBOX-VP05.OSUMC.EDU> I have used this for IHC, as well. It's great stuff! You can also use other, very viscous mounting medias, but the time it takes to dry/soak are longer than with Mount-Quick! Thanks, Bonnie -----Original Message----- From: Shirley A. Powell [mailto:POWELL_SA at mercer.edu] Sent: Friday, May 01, 2015 4:27 PM To: James Watson; 'Jb'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue transfer method: With Mount Quick you can also decolorize the section and do a different stain on them. I have even performed IHC on these transferred sections when needed. Shirley Powell -----Original Message----- From: James Watson [mailto:JWatson at gnf.org] Sent: Friday, May 01, 2015 4:21 PM To: 'Jb'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Tissue transfer method: Slide Repair 1. Soak coverslip off of slide in xylene. 2. Piece slide back together. 3. Apply Mount-Quick to slide, covering tissue. Allow to dry overnight. 4. Place slide in cold water in refrigerator overnight. 5. Carefully peal the Mount-Quick off the slide. The tissue should come up with the Mount-Quick, if it does not stop pealing up the Mount-Quick and place back into the water overnight again. 6. Carefully trim excess Mount-Quick from areas where tissue is not present. Leave at least ? inch of Mount-Quick around the tissue 7. On a new slide that is wet with water, place the removed Mount-Quick and tissue in the center of the slide (make sure the tissue is next to the slide), removing as many air bubbles from under the Mount-Quick as possible. 8. Cover the slide with a wet piece of filter paper then place a blank slide on top. If you have multiple slides to repair then they can be stacked. Apply weight to the top of this and let stand overnight. 9. Soak the dry slide in Xylene until the Mount-Quick is removed and coverslip as usual. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson at gnf.org -----Original Message----- From: Jb [mailto:craigak12 at gmail.com] Sent: Friday, May 01, 2015 1:10 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Tissue transfer method: Does anyone have a procedure that they can share on tissue transfer? One stained slide to another? Thank you for your help. Sincerely, Craig Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=VtchPmYfwzJbDOUXGIQ85Zgy9x3jvjqfbP0HekAs5UQ&s=kIfbRG7j3tUkCk3OVTZFiiD1QZ8UAahPIuyyIToZwOo&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=VtchPmYfwzJbDOUXGIQ85Zgy9x3jvjqfbP0HekAs5UQ&s=kIfbRG7j3tUkCk3OVTZFiiD1QZ8UAahPIuyyIToZwOo&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=VtchPmYfwzJbDOUXGIQ85Zgy9x3jvjqfbP0HekAs5UQ&s=kIfbRG7j3tUkCk3OVTZFiiD1QZ8UAahPIuyyIToZwOo&e= From tony.henwood at health.nsw.gov.au Fri May 1 15:42:45 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 1 May 2015 20:42:45 +0000 Subject: [Histonet] FW: IHC and oven temperature In-Reply-To: <7300978f470c4.5542734a@uwo.ca> References: <6D6BD1DE8A5571489398B392A38A7157F53EAC93@xmdb04.nch.kids> <6D6BD1DE8A5571489398B392A38A7157F53EBED0@xmdb04.nch.kids> <6D6BD1DE8A5571489398B392A38A7157F53EBEE3@xmdb04.nch.kids> <72e099b337665.5542a27d@uwo.ca> <7210a7223599c.5542a2b9@uwo.ca> <72e08fcf34e58.5542a2f8@uwo.ca> <72f0f7cd31dc1.5542a336@uwo.ca> <71f0e3043462f.5542a375@uwo.ca> <7360adfc33a1b.5542a3b3@uwo.ca> <7300aeff36043.5542a3f1@uwo.ca> <7390d1de33b0b.5542a430@uwo.ca> <72e0ce7936014.5542a46e@uwo.ca> <72e0cb853420d.5542a6a5@uwo.ca> <72b08bb645783.5542b1f1@uwo.ca> <73e0efd541544.5542b22f@uwo.ca> <72909be645f99.5542b26e@uwo.ca> <7290e2e945983.5542b2ad@uwo.ca> <7330860e45333.5542b2eb@uwo.ca> <73309c9544dac.5542b32a@uwo.ca> <72b0cf3940226.5542b368@uwo.ca> <72a0a3e5477fa.5542b3a6@uwo.ca> <73c0a23445420.5542b3e5@uwo.ca> <72b0c16445f75.5542b45f@uwo.ca> <7340cbf544835.5542b562@uwo.ca> <73c0a79d47f73.5542b8ad@uwo.ca> <73e0bb0c422f0.5542b8eb@uwo.ca> <7380ee8540fbc.5542b92a@uwo.ca> <7380c51441965.5542b968@uwo.ca> <7380ddcb419ec.5542b98d@uwo.ca>, <7300978f470c4.5542734a@uwo.ca> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53EC54D@xmdb04.nch.kids> I assume that the dako manual is referring to dry heating above 60oC, not HIER which is done in an aqueous environment. The paper I quoted (which I will send you) found that it depended on the Ag-Ab combination that was being used, some antigens were irretrievable after dry heating where as others were un-affected. Also, my caveat on this, is the importance that formalin cross-linking has in protecting some antigens from the denaturation of high temperature drying, which may not have been investigated as yet. Regards Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: John Kiernan [jkiernan at uwo.ca] Sent: Friday, 1 May 2015 9:24 AM To: Histonet at lists.utsouthwestern.edu; Tony Henwood (SCHN) Subject: Re: FW: [Histonet] IHC and oven temperature The statement quoted by Tony from the Dako manual cannot be true because many antigens have to be exposed to water at 100C in order to be immunostained - antigen retrieval. Denaturation of a macromolecule by heat increases the number of exposed epitopes, which typically are short amino acid sequences that bind specifically to the Fab segments of antibody molecules. On the other hand, it is easy to believe that 60C would denature antibody molecules enough to damage their binding sites and impair or prevent immunostaining. According to AWP Vermeer and W Norde (2000), the Fab segments of IgG were denatured when the temperature of a solution slightly exceeded 60C. ("The Thermal Stability of Immunoglobulin: Unfolding and Aggregation of a Multi-Domain Protein" Biophysical Journal 78: 394?404.) They found that further heating denatured the Fc segment, but the changed molecules became entangled and aggregated before denaturation was complete. Microwave heating is sometimes used to accelerate immunostaining, but control of the temperature is critical. For example: ME Boon & E Marani (1991) "The major importance of temperature data in publications concerning microwave techniques" European Journal of Morphology 29: 181?183. John Kiernan London, Canada = = = On 30/04/15, "Tony Henwood (SCHN)" wrote: Yes, I read the Dako IPX educational guides (5th ed) and on page 32: "No processes should raise tissue temperature to higher than 60oC as this will cause severe loss of antigenicity that may not be recoverable" Unfortunately there is no evidence given or cited that validates this statement. Even though this could be right (and there are several papers that have looked at this), this statement is scientifically weak and we should not cite this as truth. Now I do recommend the Dako reference series to my students, and I have contributed to one of these texts myself (Microscopic control of routine H&E - know your histology) but I request my students to continue to question what they read and confirm the scientific validity of the information. Regards, Tony ________________________________________ From: Joelle Weaver [joelleweaver at hotmail.com] Sent: Saturday, 25 April 2015 5:51 AM To: Tony Henwood (SCHN); WILLIAM DESALVO; Preiszner, Johanna Cc: histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] IHC and oven temperature I remember reading that the preferred temperature was about 60 degrees Celsius. I think that this was in the Dako education guides if I'm not mistaken. If that is the case, the citation for the source is probably in that resource available as pdf from their website. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tony.henwood at health.nsw.gov.au > To: wdesalvo.cac at outlook.com; PREISZNE at mail.etsu.edu > Date: Fri, 24 Apr 2015 09:43:59 +0000 > Subject: RE: [Histonet] IHC and oven temperature > CC: histonet at lists.utsouthwestern.edu > > Hi temp drying shown to be a bad idea: > > Henwood, A., (2005) ?Effect of Slide Drying at 80?C on Immunohistochemistry? J Histotechnol 28(1):45-46. > > Abstract > > Prolonged high temperature dry heating has been found to be deleterious to the immunohistochemical demonstration of several antigens in formalin-fixed, paraffin- embedded sections. Paraffin sections were dried at 80?C for 7 h and their immunoreactivity was compared with mirror sections dried for 1 h at 60?C. NCL-5D3, CMV, S100, HMB45, and CEA were quite labile to dry overheating whereas AElAE3, HBsAg, HBcAg, HSVII, EMA, chromogranin, and NSE were found to be quite resistant. It is recommended that coated slides (poly-L-lysine or aminopropyltriethoxysilane) and low-temperature drying (<60?C) be routinely used for irnmunohistochemistry. > > ________________________________________ > From: histonet-bounces at lists.utsouthwestern.edu [histonet-bounces at lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO [wdesalvo.cac at outlook.com] > Sent: Tuesday, 21 April 2015 1:56 AM > To: Preiszner, Johanna > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] IHC and oven temperature > > Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes > > Sent from my iPhone > > > On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna wrote: > > > > Hi Netters, > > > > is there something wrong with this logic: > > > > "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven." > > > > Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer... > > > > Thanks! > > > > Hanna Preiszner > > ETSU/QCOM > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From amosbrooks at gmail.com Fri May 1 15:51:00 2015 From: amosbrooks at gmail.com (Amos Brooks) Date: Fri, 1 May 2015 16:51:00 -0400 Subject: [Histonet] Tissue transfer method: Message-ID: Hi, I have used a product called Mount Quick. It is designed as a liquid coverslip, but once applied, it can be removed lifting the sections along with it. Here is a link with a good description of the process. http://www.newcomersupply.com/product/mount-quick Happy Friday, Amos Brooks On Fri, May 1, 2015 at 4:27 PM, wrote: > Message: 10 > Date: Fri, 1 May 2015 13:10:19 -0700 > From: Jb > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Tissue transfer method: > Message-ID: > Content-Type: text/plain; charset=us-ascii > > Does anyone have a procedure that they can share on tissue transfer? One > stained slide to another? > > Thank you for your help. > > Sincerely, > > Craig > From tony.henwood at health.nsw.gov.au Fri May 1 15:51:07 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 1 May 2015 20:51:07 +0000 Subject: [Histonet] GMS Question In-Reply-To: References: Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53EC593@xmdb04.nch.kids> I would strongly suggest that you not use Periodic acid for the GMS, its alright for the PAS but for the GMS. Use chromic acid as described the standard texts. Regards Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Paula Lucas [plucas at biopath.org] Sent: Saturday, 2 May 2015 12:16 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] GMS Question Hello, I think I already know the answer but I'm not sure why so if someone can help me understand the theory behind it, I would greatly appreciate it. Currently, we use the Richard Allen kit for the GMS stain and it uses Periodic Acid as the 1st step. We use a control tissue from a case we had that was positive for fungus and it's a fungus ball from the Rt Maxillary. We ran a test for fungus on a different and current case of the same tissue (different patient): Rt Maxillary sinus. The control tissue did work, but the patient's tissue did not, so the doctor ordered a PAS for fungus and this clearly showed the fungal elements nicely. My question is why would the control and patient tissue have different results when they are both fungus balls from the same specimen source? Thanks in advance, Paula Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood at health.nsw.gov.au Fri May 1 16:13:29 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 1 May 2015 21:13:29 +0000 Subject: [Histonet] long answer RE: GMS Question In-Reply-To: <001601d0842e$fc45f6b0$f4d1e410$@bresnan.net> References: <001601d0842e$fc45f6b0$f4d1e410$@bresnan.net> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53EC5BA@xmdb04.nch.kids> I totally agree and if I might add: Pneumocystis jiroveci are difficult to see after PAS staining because of the strong mucin background staining. GMS is also more advantageous because it stains old and non-viable fungal elements more efficiently than PAS (Henwood, A. F., Prasad, L., & Bourke, V. M. (2013). The application of heated detergent dewaxing and rehydration to techniques for the demonstration of fungi: a comparison to routine xylene-alcohol dewaxing. Journal of Histotechnology, 36(2), 45-50). Also be aware of pseudo-fungi (eg Russell bodies), things that look like fungi are PAS positive but GMS negative. Pseudo-fungi will give a positive reaction with a GMS stain using periodic acid instead of chromic acid Regards Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Gayle Callis [gayle.callis at bresnan.net] Sent: Saturday, 2 May 2015 2:50 AM To: 'Paula Lucas'; histonet at lists.utsouthwestern.edu Subject: [Histonet] long answer RE: GMS Question Hi Paula, The same site of infection for your control and patient tissues does not mean the fungus species was the same. Now for addtional commentary. The classic GMS uses chromic acid as the oxidizer, stronger than periodic acid. This is something to beware of since you had a false negative result with the kit. Periodic acid should be freshly made up from periodic acid crystals just before use (Culling insisted on fresh periodic acid reagent) then discarded after that day - something that is not going on with a kit where all components are sent to you in ready to use liquid form. I think if you had used the classic GMS with chromic acid to start with, you would have had the results seen with Periodic Acid/Schiffs. Part of the problem is the not all fungus species stain well either PAS or Periodic acid/GMS, and even classic GMS. All these factors can present a problem when trying to diagnose fungal infections. Lee Luna in AFIP manual, pointed out "it has been found that the time of exposure to methenamine-silver nitrate solution for complete development may vary according to type and/or strains suspected." He advised if Nocardia asteroides is suspected, then two slides should be run: one for 60 minutes and one for 90 min in the silver solution. Histotechs should be aware these possible problems. Fungi are difficult at best even when doing fungus cultures and treat. Fortunately, there are antibodies for some fungi i.e. Aspergillus, Candida) but you would probably need the specific fungus as a control for the antibody being used. Fungus IHC experts can weigh in on the latter topic. I don't know what fungus species was in your "fungus ball" control block, but our clinical lab fungus ball control contained Aspergillus sp. from a post mortem human lung. With the researcher, he only had pure Aspergillus sp. infecting the tissues. It would be nice to know what species of fungus is in your control tissue block???? The publication by Frieda Carson along with Jerry Fredenburgh and John Maxwell "Inconsistent detection of Histoplasma capsulatum with periodic acid in GMS fungus stain" , J Histotechnology, 1999 is a profound statement about what you just experienced. Their tissue control i.e. Aspergillus sp. stained adequately with periodic acid/GMS but the H. capsulatum fungus in tissue did not stain. They studied several different times, temperatures, periodic acid concentrations and fungus species. They had additional controls containing fungi other than Aspergillus sp. for better sampling of PA/GMS on different fungi. Having different fungus species control blocks is a luxury many labs do not enjoy. The reason chromic acid is not in kits is due to shipping, health and safety hazards, must be handled carefully and collected for proper, safe disposal so it is easier to make up "GMS" kits with periodic acid. If you have to collect your silver solutions for safe chemical disposal, then chromic acid shouldn't be a big problem to do the same. Also, since Periodic acid/Schiffs is popular and commonly used for fungus staining, PAS can also present false negative results or weak staining due to the weaker periodic acid oxidation, even when the periodic acid is made in house, fresh for the day. Chromic acid/Schiffs has been recommended by people on Histonet to improve fungus staining over PAS. PAS stain always seem to work well with Candida sp. and Aspergillus sp. but classic GMS was always better in my hands. I only used classic GMS, prepared in house, and controlled the development with a microscope to avoid under and over silver staining of fungus. I will be happy to send the Carson et al publication and scan the AFIP manual pages with photos on fungus staining by Luna. I apologize for long discourse as chromic acid/GMS stain is one of my favorite special stains to perform along with a long standing interest in fungus staining. I hope this helps. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: Paula Lucas [mailto:plucas at biopath.org] Sent: Friday, May 01, 2015 8:17 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] GMS Question Hello, I think I already know the answer but I'm not sure why so if someone can help me understand the theory behind it, I would greatly appreciate it. Currently, we use the Richard Allen kit for the GMS stain and it uses Periodic Acid as the 1st step. We use a control tissue from a case we had that was positive for fungus and it's a fungus ball from the Rt Maxillary. We ran a test for fungus on a different and current case of the same tissue (different patient): Rt Maxillary sinus. The control tissue did work, but the patient's tissue did not, so the doctor ordered a PAS for fungus and this clearly showed the fungal elements nicely. My question is why would the control and patient tissue have different results when they are both fungus balls from the same specimen source? Thanks in advance, Paula Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood at health.nsw.gov.au Fri May 1 16:22:32 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 1 May 2015 21:22:32 +0000 Subject: [Histonet] GMS Question In-Reply-To: References: , Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53EC5E7@xmdb04.nch.kids> Hi Teresa, Very interesting. Do you have any references on mature and immature fungal cell walls and how they react histochemically? Regards Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Goins, Tresa [TGoins at mt.gov] Sent: Saturday, 2 May 2015 3:28 AM To: Paula Lucas; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] GMS Question To get a positive PAS or GMS fungal stain, one must oxidize the carbohydrate in the fungal cell wall. Chromic acid is a stronger oxidizer than periodic acid, so would work better with mature fungal cell walls that are highly polymerized. Treat an immature cell wall for too long, and you may get a false negative because the carbohydrate structure no longer resembles a fungal cell wall. Tresa -----Original Message----- From: Paula Lucas [mailto:plucas at biopath.org] Sent: Friday, May 01, 2015 8:17 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] GMS Question Hello, I think I already know the answer but I'm not sure why so if someone can help me understand the theory behind it, I would greatly appreciate it. Currently, we use the Richard Allen kit for the GMS stain and it uses Periodic Acid as the 1st step. We use a control tissue from a case we had that was positive for fungus and it's a fungus ball from the Rt Maxillary. We ran a test for fungus on a different and current case of the same tissue (different patient): Rt Maxillary sinus. The control tissue did work, but the patient's tissue did not, so the doctor ordered a PAS for fungus and this clearly showed the fungal elements nicely. My question is why would the control and patient tissue have different results when they are both fungus balls from the same specimen source? Thanks in advance, Paula Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From abeharry798 at gmail.com Fri May 1 18:19:37 2015 From: abeharry798 at gmail.com (Gmail) Date: Fri, 1 May 2015 19:19:37 -0400 Subject: [Histonet] fatty tissue Protocol Message-ID: <8FCB301F-14B3-4938-ABDF-61E8C4F491E5@gmail.com> I'd like to know how many labs use an extended protocol to process fatty breast tissue. Does an extended protocol really work. I still believe if the tissue is submitted in small, thin and adequately fixed sections prior to processing there should be no need for a 16 hour processing cycle. Any thoughts. Andrea Beharry Technical specialist William Osler Health system Brampton, Ontario Sent from my iPad From suetp918 at comcast.net Fri May 1 19:10:37 2015 From: suetp918 at comcast.net (Sue) Date: Sat, 2 May 2015 00:10:37 +0000 (UTC) Subject: [Histonet] fatty tissue Protocol In-Reply-To: <8FCB301F-14B3-4938-ABDF-61E8C4F491E5@gmail.com> References: <8FCB301F-14B3-4938-ABDF-61E8C4F491E5@gmail.com> Message-ID: <594763378.2440580.1430525437286.JavaMail.zimbra@comcast.net> I agree if the tissue is cut properly you should not need an extended program but when you have residents and PA's they tend to rush and their sections are thicker. We do not have an extended program still within the CAP limits and it works quite nicely. TJUH Sue From rsrichmond at gmail.com Sat May 2 08:30:06 2015 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 2 May 2015 09:30:06 -0400 Subject: [Histonet] GMS question Message-ID: Gayle Callis's discourse on GMS staining is must reading for anyone who does the GMS stain. Freida Carson in 1999 showed the importance of using chromic acid - I was distressed that she didn't cite this paper in the 4th edition of her book. The most rigorous test of the GMS stain is probably the dead histoplasma in ancient fibrotic granulomas. I've gotten these things to turn up with GMS more than once - a finding of potential clinical importance. For control material, I don't think there's any substitute for infected human (or mammalian) tissue, preferably though not necessarily with a species identification of the fungus. I think either active histoplasmosis or invasive aspergillosis might provide the best material. The big academic centers that are still doing autopsies could be helpful in getting control material for everyone. Periodic acid should indeed be made up fresh with the dry chemical, but precision weighing isn't required - I used to keep a small plastic measure in the stock bottle so I could simply spoon out what I needed for the day. I'm not sure what the rules are for disposing of chromic acid, but it's a significant hazmat - toxic metal, strong acid, strong oxidant. I think you can neutralize it and precipitate the chromium, but I'd have to look up the method. Bob Richmond Samurai Pathologist Maryville TN ****************************************** Gayle Callis wrote: The same site of infection for your control and patient tissues does not mean the fungus species was the same. Now for addtional commentary. The classic GMS uses chromic acid as the oxidizer, stronger than periodic acid. This is something to beware of since you had a false negative result with the kit. Periodic acid should be freshly made up from periodic acid crystals just before use (Culling insisted on fresh periodic acid reagent) then discarded after that day - something that is not going on with a kit where all components are sent to you in ready to use liquid form. I think if you had used the classic GMS with chromic acid to start with, you would have had the results seen with Periodic Acid/Schiffs. Part of the problem is the not all fungus species stain well either PAS or Periodic acid/GMS, and even classic GMS. All these factors can present a problem when trying to diagnose fungal infections. Lee Luna in AFIP manual, pointed out "it has been found that the time of exposure to methenamine-silver nitrate solution for complete development may vary according to type and/or strains suspected." He advised if Nocardia asteroides is suspected, then two slides should be run: one for 60 minutes and one for 90 min in the silver solution. Histotechs should be aware these possible problems. Fungi are difficult at best even when doing fungus cultures and treat. Fortunately, there are antibodies for some fungi i.e. Aspergillus, Candida) but you would probably need the specific fungus as a control for the antibody being used. Fungus IHC experts can weigh in on the latter topic. I don't know what fungus species was in your "fungus ball" control block, but our clinical lab fungus ball control contained Aspergillus sp. from a post mortem human lung. With the researcher, he only had pure Aspergillus sp. infecting the tissues. It would be nice to know what species of fungus is in your control tissue block???? The publication by Freida Carson along with Jerry Fredenburgh and John Maxwell "Inconsistent detection of Histoplasma capsulatum with periodic acid in GMS fungus stain" , J Histotechnology, 1999 is a profound statement about what you just experienced. Their tissue control i.e. Aspergillus sp. stained adequately with periodic acid/GMS but the H. capsulatum fungus in tissue did not stain. They studied several different times, temperatures, periodic acid concentrations and fungus species. They had additional controls containing fungi other than Aspergillus sp. for better sampling of PA/GMS on different fungi. Having different fungus species control blocks is a luxury many labs do not enjoy. The reason chromic acid is not in kits is due to shipping, health and safety hazards, must be handled carefully and collected for proper, safe disposal so it is easier to make up "GMS" kits with periodic acid. If you have to collect your silver solutions for safe chemical disposal, then chromic acid shouldn't be a big problem to do the same. Also, since Periodic acid/Schiffs is popular and commonly used for fungus staining, PAS can also present false negative results or weak staining due to the weaker periodic acid oxidation, even when the periodic acid is made in house, fresh for the day. Chromic acid/Schiffs has been recommended by people on Histonet to improve fungus staining over PAS. PAS stain always seem to work well with Candida sp. and Aspergillus sp. but classic GMS was always better in my hands. I only used classic GMS, prepared in house, and controlled the development with a microscope to avoid under and over silver staining of fungus. I will be happy to send the Carson et al publication and scan the AFIP manual pages with photos on fungus staining by Luna. I apologize for long discourse as chromic acid/GMS stain is one of my favorite special stains to perform along with a long standing interest in fungus staining. I hope this helps. Gayle M. Callis HTL/HT/MT(ASCP) From MICHELLE.LAMPHERE at childrens.com Sat May 2 09:38:25 2015 From: MICHELLE.LAMPHERE at childrens.com (Michelle Lamphere) Date: Sat, 2 May 2015 14:38:25 +0000 Subject: [Histonet] Software for Tracking/Archiving Long-term Storage of Various Specimens In-Reply-To: References: Message-ID: What are people using to keep track of tissue specimens that they archive? We have an archive of wet and frozen tissues going back to the early 90's and are looking into a software program that will allow us to keep track of these specimens. We would also like to then generate a variety of reports based on the tissue that we have archived. Ideally, it would be nice if the program interfaced with EPIC Beaker, but that is not essential. Within the program, we would like to be able to delineate different categories that the specimens might fit into. Does such a program exist? If you are currently using a program, what do you like? And what are the limitations? We are currently in the process of implementing AB&T with Cerner, but will be moving to Epic in the next couple of years. Thank you in advance Michelle Lamphere Senior Tech, Histology Anatomic Pathology O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere at childrens.com 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at privacy at childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at privacy at childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From ian.bernard at comcast.net Sat May 2 09:44:29 2015 From: ian.bernard at comcast.net (ian bernard) Date: Sat, 2 May 2015 08:44:29 -0600 Subject: [Histonet] Bleach as Cleaning Agent or Decontamination Message-ID: <012501d084e6$7e9c9580$7bd5c080$@comcast.net> Our facility is moving towards standardization of decontaminants or disinfectants. They prefer all areas use a Sani wipes that kills most pathogens. However, we contend for Anatomic pathology we need our liquid bleach not only as disinfectant or decontaminant but as a cleaning agent for stained lab-ware. You thoughts? Also, what concentration of Bleach (5.25 or 10%) is acceptable for use as both a disinfectant and cleaning agent or should we keep them separate? We used to buy the hospital grade premade bleach at a 5.25% concentration but now they want us instead to buy the 99.9% commercial Bleach and dilute from there. Any suggestions on opaque containers for us to purchase since bleach break down after a time period, at least for disinfecting? V/r Ian From gayle.callis at bresnan.net Sat May 2 11:03:35 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Sat, 2 May 2015 10:03:35 -0600 Subject: [Histonet] Passing onto PAS discussion RE: GMS question Message-ID: <000b01d084f1$8df346e0$a9d9d4a0$@bresnan.net> Bob, Culling said exactly the same thing about not needing precision weighing for periodic acid. I discovered, by a huge error in PAS staining results, that pre-weighing 1 gm of PA into a clear sample vial and letting the pre-weighed PA stand at RT should NOT be done. The PA degraded to the point of very poor staining. I thought I was doing myself a favor to have faster solution preparation, and the pre-weighing efficiency idea was abandoned. We sometimes learn the hard way. Daily freshness is the key and we liked 1% periodic acid (or 0.5% per the original McManus and Mowry method). Our lab used 1% PA for a standard PAS stain for 10 minutes (never used for fungus) most of the time. It is interesting to note how many variations of the PAS stain exist in this world. It's important to check what PAS method variation works best for the component you are looking for as one variation may not be the best for the tissue component you want to see. If over oxidation is suspected, reduce oxidation time to 5 min (original McManus/Mowry method) and/or reduce concentration of PA to 0.5% periodic acid instead of 1% PA OR do both. Good positive controls are mandatory for any particular tissue component in mind. The only time we used a periodic acid/methenamine silver staining was for 2 micrometer renal biopsies aka Jones Basement membrane stain, and our methenamine silver was kept fresh with a 6 month expiration date on in house preparation. Fresh methenamine silver stock solution is important too. Chromic acid cannot be drain dumped and hopefully people will have hazmat collection available. If you have to collect DAB, silver solutions, then chromic acid (chromium trioxide is the stock chemical) can be collected. It isn't that difficult. Thanks for the supportive comment on GMS. Your comments on Histoplasma sp. staining were interesting as not many of us have the opportunity to work with ancient tissues. Gayle Callis -----Original Message----- From: Bob Richmond [mailto:rsrichmond at gmail.com] Sent: Saturday, May 02, 2015 7:30 AM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] GMS question Gayle Callis's discourse on GMS staining is must reading for anyone who does the GMS stain. Freida Carson in 1999 showed the importance of using chromic acid - I was distressed that she didn't cite this paper in the 4th edition of her book. The most rigorous test of the GMS stain is probably the dead histoplasma in ancient fibrotic granulomas. I've gotten these things to turn up with GMS more than once - a finding of potential clinical importance. For control material, I don't think there's any substitute for infected human (or mammalian) tissue, preferably though not necessarily with a species identification of the fungus. I think either active histoplasmosis or invasive aspergillosis might provide the best material. The big academic centers that are still doing autopsies could be helpful in getting control material for everyone. Periodic acid should indeed be made up fresh with the dry chemical, but precision weighing isn't required - I used to keep a small plastic measure in the stock bottle so I could simply spoon out what I needed for the day. I'm not sure what the rules are for disposing of chromic acid, but it's a significant hazmat - toxic metal, strong acid, strong oxidant. I think you can neutralize it and precipitate the chromium, but I'd have to look up the method. Bob Richmond Samurai Pathologist Maryville TN ****************************************** Gayle Callis wrote: The same site of infection for your control and patient tissues does not mean the fungus species was the same. Now for addtional commentary. The classic GMS uses chromic acid as the oxidizer, stronger than periodic acid. This is something to beware of since you had a false negative result with the kit. Periodic acid should be freshly made up from periodic acid crystals just before use (Culling insisted on fresh periodic acid reagent) then discarded after that day - something that is not going on with a kit where all components are sent to you in ready to use liquid form. I think if you had used the classic GMS with chromic acid to start with, you would have had the results seen with Periodic Acid/Schiffs. Part of the problem is the not all fungus species stain well either PAS or Periodic acid/GMS, and even classic GMS. All these factors can present a problem when trying to diagnose fungal infections. Lee Luna in AFIP manual, pointed out "it has been found that the time of exposure to methenamine-silver nitrate solution for complete development may vary according to type and/or strains suspected." He advised if Nocardia asteroides is suspected, then two slides should be run: one for 60 minutes and one for 90 min in the silver solution. Histotechs should be aware these possible problems. Fungi are difficult at best even when doing fungus cultures and treat. Fortunately, there are antibodies for some fungi i.e. Aspergillus, Candida) but you would probably need the specific fungus as a control for the antibody being used. Fungus IHC experts can weigh in on the latter topic. I don't know what fungus species was in your "fungus ball" control block, but our clinical lab fungus ball control contained Aspergillus sp. from a post mortem human lung. With the researcher, he only had pure Aspergillus sp. infecting the tissues. It would be nice to know what species of fungus is in your control tissue block???? The publication by Freida Carson along with Jerry Fredenburgh and John Maxwell "Inconsistent detection of Histoplasma capsulatum with periodic acid in GMS fungus stain" , J Histotechnology, 1999 is a profound statement about what you just experienced. Their tissue control i.e. Aspergillus sp. stained adequately with periodic acid/GMS but the H. capsulatum fungus in tissue did not stain. They studied several different times, temperatures, periodic acid concentrations and fungus species. They had additional controls containing fungi other than Aspergillus sp. for better sampling of PA/GMS on different fungi. Having different fungus species control blocks is a luxury many labs do not enjoy. The reason chromic acid is not in kits is due to shipping, health and safety hazards, must be handled carefully and collected for proper, safe disposal so it is easier to make up "GMS" kits with periodic acid. If you have to collect your silver solutions for safe chemical disposal, then chromic acid shouldn't be a big problem to do the same. Also, since Periodic acid/Schiffs is popular and commonly used for fungus staining, PAS can also present false negative results or weak staining due to the weaker periodic acid oxidation, even when the periodic acid is made in house, fresh for the day. Chromic acid/Schiffs has been recommended by people on Histonet to improve fungus staining over PAS. PAS stain always seem to work well with Candida sp. and Aspergillus sp. but classic GMS was always better in my hands. I only used classic GMS, prepared in house, and controlled the development with a microscope to avoid under and over silver staining of fungus. I will be happy to send the Carson et al publication and scan the AFIP manual pages with photos on fungus staining by Luna. I apologize for long discourse as chromic acid/GMS stain is one of my favorite special stains to perform along with a long standing interest in fungus staining. I hope this helps. Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver at hotmail.com Sat May 2 15:55:06 2015 From: joelleweaver at hotmail.com (Joelle Weaver) Date: Sat, 2 May 2015 20:55:06 +0000 Subject: [Histonet] IHC and oven temperature In-Reply-To: <7300978f470c4.5542734a@uwo.ca> References: , , <6D6BD1DE8A5571489398B392A38A7157F53EAC93@xmdb04.nch.kids>, , <6D6BD1DE8A5571489398B392A38A7157F53EBED0@xmdb04.nch.kids>, <6D6BD1DE8A5571489398B392A38A7157F53EBEE3@xmdb04.nch.kids>, <72e099b337665.5542a27d@uwo.ca> <7210a7223599c.5542a2b9@uwo.ca>,<72e08fcf34e58.5542a2f8@uwo.ca> <72f0f7cd31dc1.5542a336@uwo.ca>,<71f0e3043462f.5542a375@uwo.ca> <7360adfc33a1b.5542a3b3@uwo.ca>,<7300aeff36043.5542a3f1@uwo.ca> <7390d1de33b0b.5542a430@uwo.ca>,<72e0ce7936014.5542a46e@uwo.ca> <72e0cb853420d.5542a6a5@uwo.ca>,<72b08bb645783.5542b1f1@uwo.ca> <73e0efd541544.5542b22f@uwo.ca>,<72909be645f99.5542b26e@uwo.ca> <7290e2e945983.5542b2ad@uwo.ca>,<7330860e45333.5542b2eb@uwo.ca> <73309c9544dac.5542b32a@uwo.ca>,<72b0cf3940226.5542b368@uwo.ca> <72a0a3e5477fa.5542b3a6@uwo.ca>,<73c0a23445420.5542b3e5@uwo.ca> <72b0c16445f75.5542b45f@uwo.ca>,<7340cbf544835.5542b562@uwo.ca> <73c0a79d47f73.5542b8ad@uwo.ca>,<73e0bb0c422f0.5542b8eb@uwo.ca> <7380ee8540fbc.5542b92a@uwo.ca>,<7380c51441965.5542b968@uwo.ca> <7380ddcb419ec.5542b98d@uwo.ca>,<7300978f470c4.5542734a@uwo.ca> Message-ID: John your points do seem to make it seem somewhat counter-intuitive in regards to the temperatures suggested in the literature for high points for each step. Perhaps someone will be able to provide a complete theoretical basis for the differences. It seems though that there wouldn't be much of a directed point or purpose in heating slides dry at such high temperature for very long at that stage in the process. But during AR , we have moist and the eletrolytic conditions, so use of the higher temperature is applied for a more directed and specific effect that benefits us in identifying the particular epitope of interest..any thoughts? Seems like high temp acheives a goal in the second instance, but not much purpose is gained at the higher temperature in the first instance, and potentially damage to some aspects. I'll await further information and discussion from the group. Thanks Joelle Weaver MAOM, HTL (ASCP) QIHC > From: jkiernan at uwo.ca > To: Histonet at lists.utsouthwestern.edu; tony.henwood at health.nsw.gov.au > Date: Thu, 30 Apr 2015 18:24:10 -0500 > Subject: Re: FW: [Histonet] IHC and oven temperature > CC: > > The statement quoted by Tony from the Dako manual cannot be true because many antigens have to be exposed to water at 100C in order to be immunostained - antigen retrieval. Denaturation of a macromolecule by heat increases the number of exposed epitopes, which typically are short amino acid sequences that bind specifically to the Fab segments of antibody molecules. > > On the other hand, it is easy to believe that 60C would denature antibody molecules enough to damage their binding sites and impair or prevent immunostaining. According to AWP Vermeer and W Norde (2000), the Fab segments of IgG were denatured when the temperature of a solution slightly exceeded 60C. ("The Thermal Stability of Immunoglobulin: Unfolding and Aggregation of a Multi-Domain Protein" Biophysical Journal 78: 394?404.) They found that further heating denatured the Fc segment, but the changed molecules became entangled and aggregated before denaturation was complete. Microwave heating is sometimes used to accelerate immunostaining, but control of the temperature is critical. For example: ME Boon & E Marani (1991) "The major importance of temperature data in publications concerning microwave techniques" European Journal of Morphology 29: 181?183. > > John Kiernan > London, Canada > = = = > On 30/04/15, "Tony Henwood (SCHN)" wrote: > > > > Yes, > > > > I read the Dako IPX educational guides (5th ed) and on page 32: > > "No processes should raise tissue temperature to higher than 60oC as this will cause severe loss of antigenicity that may not be recoverable" > > Unfortunately there is no evidence given or cited that validates this statement. Even though this could be right (and there are several papers that have looked at this), this statement is scientifically weak and we should not cite this as truth. > > > > Now I do recommend the Dako reference series to my students, and I have contributed to one of these texts myself (Microscopic control of routine H&E - know your histology) but I request my students to continue to question what they read and confirm the scientific validity of the information. > > > > Regards, > > Tony > > > > ________________________________________ > > From: Joelle Weaver [joelleweaver at hotmail.com] > > Sent: Saturday, 25 April 2015 5:51 AM > > To: Tony Henwood (SCHN); WILLIAM DESALVO; Preiszner, Johanna > > Cc: histonet at lists.utsouthwestern.edu > > Subject: RE: [Histonet] IHC and oven temperature > > > > I remember reading that the preferred temperature was about 60 degrees Celsius. I think that this was in the Dako education guides if I'm not mistaken. If that is the case, the citation for the source is probably in that resource available as pdf from their website. > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > > > > > > > From: tony.henwood at health.nsw.gov.au > > > To: wdesalvo.cac at outlook.com; PREISZNE at mail.etsu.edu > > > Date: Fri, 24 Apr 2015 09:43:59 +0000 > > > Subject: RE: [Histonet] IHC and oven temperature > > > CC: histonet at lists.utsouthwestern.edu > > > > > > Hi temp drying shown to be a bad idea: > > > > > > Henwood, A., (2005) ?Effect of Slide Drying at 80?C on Immunohistochemistry? J Histotechnol 28(1):45-46. > > > > > > Abstract > > > > > > Prolonged high temperature dry heating has been found to be deleterious to the immunohistochemical demonstration of several antigens in formalin-fixed, paraffin- embedded sections. Paraffin sections were dried at 80?C for 7 h and their immunoreactivity was compared with mirror sections dried for 1 h at 60?C. NCL-5D3, CMV, S100, HMB45, and CEA were quite labile to dry overheating whereas AElAE3, HBsAg, HBcAg, HSVII, EMA, chromogranin, and NSE were found to be quite resistant. It is recommended that coated slides (poly-L-lysine or aminopropyltriethoxysilane) and low-temperature drying (<60?C) be routinely used for irnmunohistochemistry. > > > > > > ________________________________________ > > > From: histonet-bounces at lists.utsouthwestern.edu [histonet-bounces at lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO [wdesalvo.cac at outlook.com] > > > Sent: Tuesday, 21 April 2015 1:56 AM > > > To: Preiszner, Johanna > > > Cc: histonet at lists.utsouthwestern.edu > > > Subject: Re: [Histonet] IHC and oven temperature > > > > > > Dry heat compared to wet heat. Do not "dry" your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes > > > > > > Sent from my iPhone > > > > > > > On Apr 20, 2015, at 8:48 AM, Preiszner, Johanna wrote: > > > > > > > > Hi Netters, > > > > > > > > is there something wrong with this logic: > > > > > > > > "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven." > > > > > > > > Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer... > > > > > > > > Thanks! > > > > > > > > Hanna Preiszner > > > > ETSU/QCOM > > > > > > > > > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet at lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet at lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ********************************************************************************* > > > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > > > > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > > > > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > > > ********************************************************************************* > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet at lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********************************************************************************* > > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > > ********************************************************************************* > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From annigyg at gmail.com Mon May 4 00:10:53 2015 From: annigyg at gmail.com (Anne Van Binsbergen) Date: Mon, 4 May 2015 09:10:53 +0400 Subject: [Histonet] Home made IHC breast markers: home made NBF vs Commercially prepared Message-ID: <0B902DE1-7BA9-4B90-B9E5-D0633C912CD5@gmail.com> Greetings esteemed Histonetters We use commercially prepared NBF in pre-filled containers for collection and submission of all tissue. We use 'home-made' NBF ( Millonigs as per Freda Carson) in the holding tanks on the gross station and in all our tissue processors. We also strictly adhere to CAP regulations re fixation times for all breast tissue (6-72 hrs) - - our lower end cut-off is 8 hrs. Question: Could the use of 2 'different ' sources of NBF affect the staining of predictive markers? Your expert opinion is highly appreciated Regards from SandyLands annieinarabia Sent from my iPhone From b-frederick at northwestern.edu Mon May 4 09:53:07 2015 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Mon, 4 May 2015 14:53:07 +0000 Subject: [Histonet] MTA-1 Microarrayer Message-ID: Hello all, Anybody have any idea where to get the batteries for this arrayer? Regular 2450 batteries are too thick. Batteries plus was a no go. See some on Amazon but not sure if they are thin enough. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu From tp2 at medicine.wisc.edu Mon May 4 10:11:21 2015 From: tp2 at medicine.wisc.edu (Thomas Pier) Date: Mon, 04 May 2015 10:11:21 -0500 Subject: [Histonet] MTA-1 Microarrayer Message-ID: <554745CD020000DF00016D86@gwmail2.medicine.wisc.edu> You're going to want to look for the Renata brand batteries. They're a bit thinner and fit the MTA-1 perfectly. Tom >>> Bernice Frederick 05/04/15 9:58 AM >>> Hello all, Anybody have any idea where to get the batteries for this arrayer? Regular 2450 batteries are too thick. Batteries plus was a no go. See some on Amazon but not sure if they are thin enough. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor at jhmi.edu Mon May 4 10:22:25 2015 From: hfedor at jhmi.edu (Helen Fedor) Date: Mon, 4 May 2015 15:22:25 +0000 Subject: [Histonet] MTA-1 Microarrayer In-Reply-To: References: Message-ID: Hello Bernice, we have tried out several and found that "enercell" works. CR2450 their number is 23-808. WE might have found them on amazon. Cannot remember. Helen L. Fedor Oncology Tissue Services, Manager Johns Hopkins University 411 N. Caroline St? Room 310 Basement| Bond St Annex Building Baltimore, MD?| 21231 410-614-1660 http://tmalab.jhmi.edu/ -----Original Message----- From: Bernice Frederick [mailto:b-frederick at northwestern.edu] Sent: Monday, May 04, 2015 10:53 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] MTA-1 Microarrayer Hello all, Anybody have any idea where to get the batteries for this arrayer? Regular 2450 batteries are too thick. Batteries plus was a no go. See some on Amazon but not sure if they are thin enough. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV at archildrens.org Mon May 4 11:30:46 2015 From: HornHV at archildrens.org (Horn, Hazel V) Date: Mon, 4 May 2015 11:30:46 -0500 Subject: [Histonet] zero based position - Arkansas Message-ID: <25A4DE08332B19499904459F00AAACB71A1EB2553D@EVS1.archildrens.org> Arkansas Children's Hospital in Little Rock will have a zero based position posted this week. This is a temporary position that could possibly work into a fulltime position if you are the right person for the job. The zero based position will have no benefits. The hours will be from 7:00am until 1:00pm, Monday through Friday. Please contact me directly if you are interested. Thanks. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From jpiche at wtbyhosp.org Mon May 4 12:27:16 2015 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Mon, 4 May 2015 17:27:16 +0000 Subject: [Histonet] IHC billing question In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E3E1493EE@HHCEXCHMB03.hhcsystem.org> <25A4DE08332B19499904459F00AAACB71A1EB25529@EVS1.archildrens.org> <00b201d08431$1dd819f0$59884dd0$@pathview.com> Message-ID: <631955447A364B45B9458D2905635110D8C4CF78@WIN08-MBX-01.wtbyhosp.org> Hi Joyce, What about if you are using 88360 for Estrogen receptor and Progesterone receptor? An example would be an order for ER, PR, p63, AE1/3? Would it be 88360 x 2, 88342, 88341 or would be it 88360 x 2 and 88341 x 2? I'm not sure if the quantitative charge takes precedence over the 88342 being the first identifiable antibody or if they are stand alone? Thanks, Jessica Piche,HT (ASCP) Waterbury Hospital -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] Sent: Friday, May 01, 2015 2:22 PM To: 'Michael Mihalik'; 'Horn, Hazel V'; 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing question It's per specimen - 88342 for the first ab on a specimen, 88341 for ea additional on same specimen. (Not multiple blocks on same specimen). Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Michael Mihalik [mailto:mike at pathview.com] Sent: Friday, May 01, 2015 1:06 PM To: 'Horn, Hazel V'; Weems, Joyce K.; 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] IHC billing question Am I misunderstanding, or should it be per specimen and not per case? Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Horn, Hazel V [mailto:HornHV at archildrens.org] Sent: Friday, May 01, 2015 8:50 AM To: 'Weems, Joyce K.'; 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing question We have 2 billing codes for IHC. One for the first IHC and another for all additional IHC's on the same case. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] Sent: Friday, May 01, 2015 8:13 AM To: 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC billing question I do it every day - change every first to 88342. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, April 30, 2015 5:33 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC billing question Effective January 1, 2015, our LIS team removed all of the CPT 88342 codes for IHC from our CoPath stain dictionary since you couldn't tell whether a Cytokeratin-7 was being performed as an "88341" or as an "88342". Now, as you might have expected, none of the "inpatient" IHC testing has been accounted for (the outpatient IHC has been billed manually from the pathology report), and they want someone to go back and enter all the CPT codes into the system (hopefully, not me!). Has anyone else encountered this problem? Thanks (I think). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From jpiche at wtbyhosp.org Mon May 4 12:32:42 2015 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Mon, 4 May 2015 17:32:42 +0000 Subject: [Histonet] Centrifuge for cytospins In-Reply-To: <3C2378778400AD448ADA6FD6BDB7CCCC1D8ECEAE@RRMBX03.rrmc.local> References: <3C2378778400AD448ADA6FD6BDB7CCCC1D8ECEAE@RRMBX03.rrmc.local> Message-ID: <631955447A364B45B9458D2905635110D8C4DFCA@WIN08-MBX-01.wtbyhosp.org> Thanks for sharing Joe! :) Jessica -----Original Message----- From: Joe W. Walker, Jr. [mailto:joewalker at rrmc.org] Sent: Tuesday, April 28, 2015 2:31 PM To: Cooper, Brian; Piche, Jessica; histonet at lists.utsouthwestern.edu Subject: RE: Centrifuge for cytospins In all of my experiences with cytology preparations, the first step would be to centrifuge the specimen. This concentrates the cells for the next step; cytocentrifuge, which is used for the presentation of the cells for evaluation. Here is an excerpt from our procedure: 3. Label a 50 ml centrifuge tube with the cytology accession label. 4. Mix the specimen using a vortex mixer for 10-30 seconds. 5. Pour contents of specimen from the original container into the 50 ml centrifuge tube and cap the tube. 6. Centrifuge the specimen at 600g for 10 minutes (program 1) or 1200g for 5 minutes (program 2). 7. Pour off the supernatant and re-suspend the sediment in 0.5 to 1ml of Cytospin (green) fluid, depending on the cell button size. Evaluating the number of drops you might need for your cytocentrifuge can be performed by taking a crop from the sediment, placing it on a slide, placing a 24x50mm cover glass on the specimen and then observing the cellularity of the specimen. I have document that illustrates this but I don't think the list serve will allow attachments. You can email me privately for this document. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker at rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Tuesday, April 28, 2015 12:10 PM To: Piche, Jessica; histonet at lists.utsouthwestern.edu Subject: [Histonet] RE: Centrifuge for cytospins We make our BAL cytospins directly--no centrifuging first. Brian -----Original Message----- From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Tuesday, April 28, 2015 8:58 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Centrifuge for cytospins Hi Everyone, Just asking a quick question for our Cyto department. Are BAL slides centrifuged and then made in to cytospin slides or do you just make the cytospin slides with no centrifuging? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From jpiche at wtbyhosp.org Mon May 4 12:34:20 2015 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Mon, 4 May 2015 17:34:20 +0000 Subject: [Histonet] Centrifuge for cytospins In-Reply-To: References: <631955447A364B45B9458D2905635110D8C1E075@WIN08-MBX-01.wtbyhosp.org> Message-ID: <631955447A364B45B9458D2905635110D8C4DFF8@WIN08-MBX-01.wtbyhosp.org> Thanks for sharing Brian. Jessica -----Original Message----- From: Cooper, Brian [mailto:bcooper at chla.usc.edu] Sent: Tuesday, April 28, 2015 12:10 PM To: Piche, Jessica; histonet at lists.utsouthwestern.edu Subject: RE: Centrifuge for cytospins We make our BAL cytospins directly--no centrifuging first. Brian -----Original Message----- From: histonet-bounces at lists.utsouthwestern.edu [mailto:histonet-bounces at lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Tuesday, April 28, 2015 8:58 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Centrifuge for cytospins Hi Everyone, Just asking a quick question for our Cyto department. Are BAL slides centrifuged and then made in to cytospin slides or do you just make the cytospin slides with no centrifuging? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From gayle.callis at bresnan.net Mon May 4 17:21:01 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Mon, 4 May 2015 16:21:01 -0600 Subject: [Histonet] Can't log into Histonet to do anything Message-ID: <000001d086b8$9ad529f0$d07f7dd0$@bresnan.net> Dear Histonettters, At the risk of being pesky, is Histonet having problems. I generally go to Histonet via Firefox/Google and haven't been able to get to the website for two days. I only hope someone out there can even get this message. I have tried finding Marvin Hanna's email address. Stymied and dead in the water. I would love to unsubscribe, but not sure anyone gets this message. Gayle Callis From koellingr at comcast.net Mon May 4 17:55:04 2015 From: koellingr at comcast.net (koellingr at comcast.net) Date: Mon, 4 May 2015 22:55:04 +0000 (UTC) Subject: [Histonet] Can't log into Histonet to do anything In-Reply-To: <000001d086b8$9ad529f0$d07f7dd0$@bresnan.net> References: <000001d086b8$9ad529f0$d07f7dd0$@bresnan.net> Message-ID: <1500839334.3179803.1430780104720.JavaMail.zimbra@comcast.net> I got your message Gayle (through Histonet) although haven't heard much at all weekend otherwise.? Use IE. Ray? in Lake Forest Park, WA ----- Original Message ----- From: "Gayle Callis" To: "Histonet" Sent: Monday, May 4, 2015 3:21:01 PM Subject: [Histonet] Can't log into Histonet to do anything Dear Histonettters, ? At the risk of being pesky, is Histonet having problems. ? I generally go to Histonet via Firefox/Google and haven't been able to get to the website for two days. ? I only hope someone out there can even get this message. ?I have tried finding Marvin Hanna's email address. ? ? Stymied and dead in the water. ? I would love to unsubscribe, but not sure anyone gets this message. ? Gayle Callis _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper at chla.usc.edu Mon May 4 18:07:29 2015 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Mon, 4 May 2015 23:07:29 +0000 Subject: [Histonet] Can't log into Histonet to do anything In-Reply-To: <1500839334.3179803.1430780104720.JavaMail.zimbra@comcast.net> References: <000001d086b8$9ad529f0$d07f7dd0$@bresnan.net> <1500839334.3179803.1430780104720.JavaMail.zimbra@comcast.net> Message-ID: I got Gayle's email as well, but when I went to Histonet's page though Firefox and Google, got the "Unable to Connect" message when I clicked on a bunch of the Monthly hyperlinks. Same message for everything back until the Histonet, November, 2008 hyperlink. They seem to work prior to this date. Tried IE as well, same result. Brian -----Original Message----- From: koellingr at comcast.net [mailto:koellingr at comcast.net] Sent: Monday, May 04, 2015 3:55 PM To: callis, gayle Cc: Histonet Subject: Re: [Histonet] Can't log into Histonet to do anything I got your message Gayle (through Histonet) although haven't heard much at all weekend otherwise.? Use IE. Ray? in Lake Forest Park, WA ----- Original Message ----- From: "Gayle Callis" To: "Histonet" Sent: Monday, May 4, 2015 3:21:01 PM Subject: [Histonet] Can't log into Histonet to do anything Dear Histonettters, ? At the risk of being pesky, is Histonet having problems. ? I generally go to Histonet via Firefox/Google and haven't been able to get to the website for two days. ? I only hope someone out there can even get this message. ?I have tried finding Marvin Hanna's email address. ? ? Stymied and dead in the water. ? I would love to unsubscribe, but not sure anyone gets this message. ? Gayle Callis _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From mhanna at histosearch.com Mon May 4 19:40:19 2015 From: mhanna at histosearch.com (mhanna at histosearch.com) Date: Mon, 4 May 2015 20:40:19 -0400 Subject: [Histonet] Can't log into Histonet to do anything In-Reply-To: References: <000001d086b8$9ad529f0$d07f7dd0$@bresnan.net> <1500839334.3179803.1430780104720.JavaMail.zimbra@comcast.net> Message-ID: <035401d086cc$17184020$4548c060$@histosearch.com> Hi Gayle, Ray, Brian and Histonetters, It does appear the computer that runs our list server and about 20 others, lists.utsouthwestern.edu, is currently down or not answering. This server is run by the IT Department at UT Southwest. Sometimes computers crash and it can take time to fix the problem. They may also shut down less critical servers when the University needs priority on their bandwidth. I know they have some smart IT people and will probably have it back up soon. In the meantime, it won't be possible to subscribe or unsubscribe. It does look like messages are getting through, so they probably have the email routed through a different server. Hope this helps. Marvin -----Original Message----- From: Cooper, Brian [mailto:bcooper at chla.usc.edu] Sent: Monday, May 04, 2015 7:07 PM To: koellingr at comcast.net; callis, gayle Cc: Histonet Subject: Re: [Histonet] Can't log into Histonet to do anything I got Gayle's email as well, but when I went to Histonet's page though Firefox and Google, got the "Unable to Connect" message when I clicked on a bunch of the Monthly hyperlinks. Same message for everything back until the Histonet, November, 2008 hyperlink. They seem to work prior to this date. Tried IE as well, same result. Brian -----Original Message----- From: koellingr at comcast.net [mailto:koellingr at comcast.net] Sent: Monday, May 04, 2015 3:55 PM To: callis, gayle Cc: Histonet Subject: Re: [Histonet] Can't log into Histonet to do anything I got your message Gayle (through Histonet) although haven't heard much at all weekend otherwise. Use IE. Ray in Lake Forest Park, WA ----- Original Message ----- From: "Gayle Callis" To: "Histonet" Sent: Monday, May 4, 2015 3:21:01 PM Subject: [Histonet] Can't log into Histonet to do anything Dear Histonettters, At the risk of being pesky, is Histonet having problems. I generally go to Histonet via Firefox/Google and haven't been able to get to the website for two days. I only hope someone out there can even get this message. I have tried finding Marvin Hanna's email address. Stymied and dead in the water. I would love to unsubscribe, but not sure anyone gets this message. Gayle Callis _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kgrobert at rci.rutgers.edu Tue May 5 09:06:30 2015 From: kgrobert at rci.rutgers.edu (Kathleen Roberts) Date: Tue, 5 May 2015 10:06:30 -0400 (EDT) Subject: [Histonet] Can't log into Histonet to do anything Message-ID: I tried just now via Safari, and got this error message: Gateway Timeout: can't connect to remote host. Hopefully the IT people can fix it. Kathleen > I got Gayle's email as well, but when I went to Histonet's page though Firefox and Google, got the "Unable to Connect" message when I clicked on > a bunch of the Monthly hyperlinks. Same message for everything back until > the Histonet, November, 2008 hyperlink. They seem to work prior to this date. Tried IE as well, same result. > > Brian > > > -----Original Message----- > From: koellingr at comcast.net [mailto:koellingr at comcast.net] > Sent: Monday, May 04, 2015 3:55 PM > To: callis, gayle > Cc: Histonet > Subject: Re: [Histonet] Can't log into Histonet to do anything > > I got your message Gayle (through Histonet) although haven't heard much at > all weekend otherwise.?? Use IE. > Ray?? in Lake Forest Park, WA > > ----- Original Message ----- > > From: "Gayle Callis" > To: "Histonet" > Sent: Monday, May 4, 2015 3:21:01 PM > Subject: [Histonet] Can't log into Histonet to do anything > > Dear Histonettters, > > ?? > > At the risk of being pesky, is Histonet having problems. ?? I generally go > to Histonet via Firefox/Google and haven't been able to get to the website > for two days. ?? I only hope someone out there can even get this message. > ??I have tried finding Marvin Hanna's email address. ?? > > ?? > > Stymied and dead in the water. ?? I would love to unsubscribe, but not sure > anyone gets this message. > > ?? > > Gayle Callis > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original > message. > > --------------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 From Sarah.Dysart at stdavids.com Tue May 5 09:16:49 2015 From: Sarah.Dysart at stdavids.com (Sarah.Dysart at stdavids.com) Date: Tue, 5 May 2015 14:16:49 +0000 Subject: [Histonet] Can't log into Histonet to do anything In-Reply-To: References: Message-ID: <210F59E89FE7784F9FDAD2AEC2B9E15D17C9C3@FWDCWPMSGHCMD3D.hca.corpad.net> At least you can post...I've been trying to be able to post for over a month, even emailed the list serv. With no response...frustrating because I love this resource! Sarah Dysart Pathology Supervisor -----Original Message----- From: Kathleen Roberts [mailto:kgrobert at rci.rutgers.edu] Sent: Tuesday, May 05, 2015 9:07 AM To: callis, gayle; Histonet Subject: [EXTERNAL] Re: [Histonet] Can't log into Histonet to do anything I tried just now via Safari, and got this error message: Gateway Timeout: can't connect to remote host. Hopefully the IT people can fix it. Kathleen > I got Gayle's email as well, but when I went to Histonet's page though Firefox and Google, got the "Unable to Connect" message when I clicked on > a bunch of the Monthly hyperlinks. Same message for everything back until > the Histonet, November, 2008 hyperlink. They seem to work prior to > this date. Tried IE as well, same result. > > Brian > > > -----Original Message----- > From: koellingr at comcast.net [mailto:koellingr at comcast.net] > Sent: Monday, May 04, 2015 3:55 PM > To: callis, gayle > Cc: Histonet > Subject: Re: [Histonet] Can't log into Histonet to do anything > > I got your message Gayle (through Histonet) although haven't heard > much at > all weekend otherwise.?? Use IE. > Ray?? in Lake Forest Park, WA > > ----- Original Message ----- > > From: "Gayle Callis" > To: "Histonet" > Sent: Monday, May 4, 2015 3:21:01 PM > Subject: [Histonet] Can't log into Histonet to do anything > > Dear Histonettters, > > ? > > At the risk of being pesky, is Histonet having problems. ?? I > generally go > to Histonet via Firefox/Google and haven't been able to get to the website > for two days. ?? I only hope someone out there can even get this message. > ??I have tried finding Marvin Hanna's email address. ? > > ? > > Stymied and dead in the water. ?? I would love to unsubscribe, but not > sure anyone gets this message. > > ? > > Gayle Callis > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please > contact the sender by reply e-mail and destroy all copies of this original > message. > > --------------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anna.coffey at nih.gov Tue May 5 10:36:32 2015 From: anna.coffey at nih.gov (Coffey, Anna (NIH/NCI) [C]) Date: Tue, 5 May 2015 15:36:32 +0000 Subject: [Histonet] qIHC Prep Message-ID: <5C3E10119A1B824FBE92B08279F74A9101799289@msgb10.nih.gov> Histonet, I am preparing for my IHC qualification exam this summer and I am very interested to hear about what prep materials you used to prepare for the exam. I have the Carson book, the DAKO guide book, and the workbook put out by the Michigan Society of HTs. I'm wondering if any of you have other suggestions on materials that were helpful to you and if you know of any practice exams out there. I'm finding that, although the workbook is helpful, I'm not actually sure if my answers are 100% correct. I appreciate any help you can give me! Thanks, Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey at nih.gov 301-846-1730 From jkiernan at uwo.ca Tue May 5 10:49:39 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Tue, 05 May 2015 10:49:39 -0500 Subject: [Histonet] Can't log into Histonet to do anything In-Reply-To: <7250e3eb11845.5548e686@uwo.ca> References: <210F59E89FE7784F9FDAD2AEC2B9E15D17C9C3@FWDCWPMSGHCMD3D.hca.corpad.net> <7250e3eb11845.5548e686@uwo.ca> Message-ID: <7300a12116e6f.5548a043@uwo.ca> You posted this one to Histonet! = = = On 05/05/15, Sarah.Dysart at stdavids.com wrote: > At least you can post...I've been trying to be able to post for over a month, even emailed the list serv. With no response...frustrating because I love this resource! > > Sarah Dysart > Pathology Supervisor > > > -----Original Message----- > From: Kathleen Roberts [mailto:kgrobert at rci.rutgers.edu] > Sent: Tuesday, May 05, 2015 9:07 AM > To: callis, gayle; Histonet > Subject: [EXTERNAL] Re: [Histonet] Can't log into Histonet to do anything > > I tried just now via Safari, and got this error message: > > Gateway Timeout: can't connect to remote host. > > Hopefully the IT people can fix it. > > Kathleen > > > > I got Gayle's email as well, but when I went to Histonet's page though > Firefox and Google, got the "Unable to Connect" message when I clicked on > > a bunch of the Monthly hyperlinks. Same message for everything back > until > > the Histonet, November, 2008 hyperlink. They seem to work prior to > > this > date. Tried IE as well, same result. > > > > Brian > > > > > > -----Original Message----- > > From: koellingr at comcast.net [mailto:koellingr at comcast.net] > > Sent: Monday, May 04, 2015 3:55 PM > > To: callis, gayle > > Cc: Histonet > > Subject: Re: [Histonet] Can't log into Histonet to do anything > > > > I got your message Gayle (through Histonet) although haven't heard > > much > at > > all weekend otherwise.? Use IE. > > Ray? in Lake Forest Park, WA > > > > ----- Original Message ----- > > > > From: "Gayle Callis" > > To: "Histonet" > > Sent: Monday, May 4, 2015 3:21:01 PM > > Subject: [Histonet] Can't log into Histonet to do anything > > > > Dear Histonettters, > > > > ? > > > > At the risk of being pesky, is Histonet having problems. ? I > > generally > go > > to Histonet via Firefox/Google and haven't been able to get to the > website > > for two days. ? I only hope someone out there can even get this > message. > > ? I have tried finding Marvin Hanna's email address. ? > > > > ? > > > > Stymied and dead in the water. ? I would love to unsubscribe, but not > > sure anyone gets this message. > > > > ? > > > > Gayle Callis > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential > > or legally privileged information. Any unauthorized review, use, > > disclosure or distribution is prohibited. If you are not the intended > > recipient, > please > > contact the sender by reply e-mail and destroy all copies of this > original > > message. > > > > --------------------------------------------------------------------- > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > Principal Lab Technician > Neurotoxicology Labs > Dept of Pharmacology & Toxicology > Rutgers, the State University of NJ > 41 B Gordon Road > Piscataway, NJ 08854 > (848) 445-1443 > FAX (732) 445-6905 > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Sarah.Dysart at stdavids.com Tue May 5 10:52:52 2015 From: Sarah.Dysart at stdavids.com (Sarah.Dysart at stdavids.com) Date: Tue, 5 May 2015 15:52:52 +0000 Subject: [Histonet] Can't log into Histonet to do anything In-Reply-To: <7300a12116e6f.5548a043@uwo.ca> References: <210F59E89FE7784F9FDAD2AEC2B9E15D17C9C3@FWDCWPMSGHCMD3D.hca.corpad.net> <7250e3eb11845.5548e686@uwo.ca> <7300a12116e6f.5548a043@uwo.ca> Message-ID: <210F59E89FE7784F9FDAD2AEC2B9E15D17CA47@FWDCWPMSGHCMD3D.hca.corpad.net> I know...it's like taking your car to a mechanic saying it makes a noise then it doesn't...only in reverse =) Let's see if I can post an original... Sarah Dysart Pathology Supervisor From: John Kiernan [mailto:jkiernan at uwo.ca] Sent: Tuesday, May 05, 2015 10:50 AM To: Dysart Sarah; histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Can't log into Histonet to do anything You posted this one to Histonet! = = = On 05/05/15, Sarah.Dysart at stdavids.com wrote: At least you can post...I've been trying to be able to post for over a month, even emailed the list serv. With no response...frustrating because I love this resource! Sarah Dysart Pathology Supervisor -----Original Message----- From: Kathleen Roberts [mailto:kgrobert at rci.rutgers.edu] Sent: Tuesday, May 05, 2015 9:07 AM To: callis, gayle; Histonet Subject: [EXTERNAL] Re: [Histonet] Can't log into Histonet to do anything I tried just now via Safari, and got this error message: Gateway Timeout: can't connect to remote host. Hopefully the IT people can fix it. Kathleen > I got Gayle's email as well, but when I went to Histonet's page though Firefox and Google, got the "Unable to Connect" message when I clicked on > a bunch of the Monthly hyperlinks. Same message for everything back until > the Histonet, November, 2008 hyperlink. They seem to work prior to > this date. Tried IE as well, same result. > > Brian > > > -----Original Message----- > From: koellingr at comcast.net [mailto:koellingr at comcast.net] > Sent: Monday, May 04, 2015 3:55 PM > To: callis, gayle > Cc: Histonet > Subject: Re: [Histonet] Can't log into Histonet to do anything > > I got your message Gayle (through Histonet) although haven't heard > much at > all weekend otherwise.? Use IE. > Ray? in Lake Forest Park, WA > > ----- Original Message ----- > > From: "Gayle Callis" > > To: "Histonet" > > Sent: Monday, May 4, 2015 3:21:01 PM > Subject: [Histonet] Can't log into Histonet to do anything > > Dear Histonettters, > > ? > > At the risk of being pesky, is Histonet having problems. ? I > generally go > to Histonet via Firefox/Google and haven't been able to get to the website > for two days. ? I only hope someone out there can even get this message. > ? I have tried finding Marvin Hanna's email address. ? > > ? > > Stymied and dead in the water. ? I would love to unsubscribe, but not > sure anyone gets this message. > > ? > > Gayle Callis > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please > contact the sender by reply e-mail and destroy all copies of this original > message. > > --------------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght at yahoo.com Tue May 5 11:18:41 2015 From: tkngflght at yahoo.com (Cheryl) Date: Tue, 5 May 2015 09:18:41 -0700 Subject: [Histonet] Plasma/thrombin clot for cell block Message-ID: <1430842721.83074.YahooMailBasic@web161203.mail.bf1.yahoo.com> HI Guys- I need procedures including products to create a plasma cell block for non-gyn cytology. The one we have uses bovine thrombin and calcium chloride (dihydrate) but it's gotten corrupted and I can't find the original resources. Help? Cheryl tkngflght at yahoo.com From jpiche at wtbyhosp.org Tue May 5 13:18:54 2015 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Tue, 5 May 2015 18:18:54 +0000 Subject: [Histonet] All Common Question 30550 Message-ID: <631955447A364B45B9458D2905635110D8D2953C@WIN08-MBX-01.wtbyhosp.org> Hi Everyone, I was just wondering if everyone has a written procedure for performance verification for their tissue processors? And if so what your procedure entails? Performance verification reports from companies that fix the tissue processor or an actual program on the tissue processor to verify that it processed tissue correctly. Got a ding with CAP because we didn't have a procedure in regards to the tissue processors, but I thought this question in the all common checklist had to do with chemistry analyzers and chemistry's equipment in general. Wondering what others are doing. Thank you in advance!!! Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From tbraud at holyredeemer.com Tue May 5 14:30:02 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 5 May 2015 19:30:02 +0000 Subject: [Histonet] Cytology billing Message-ID: <48E053DDF6CE074DB6A7414BA05403F8E7F0@HRHEX02-HOS.holyredeemer.local> I have a question for all you cytology prep Histo folk out there. 1. If I have an FNA with 2 direct smears but NOT immediate interpretation, and an aspirate that is made into a Cell block, what will be the CPT codes charged? 2. Also, If I have an FNA with 2 direct smears for immediate interpretation, and an aspirate that will only make a thin prep, what will be the CPT codes Charged? Inquiring Histo minds need some clarity, please. Thanks, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 From bethany at mydermsurgery.com Tue May 5 15:42:14 2015 From: bethany at mydermsurgery.com (Bethany May) Date: Tue, 5 May 2015 20:42:14 +0000 Subject: [Histonet] pay rate Message-ID: <1430858522736.17787@mydermsurgery.com> Hello everyone, I have a friend in central Florida who just passed the exam for histotechnologist! Yay for her!! She was wondering what the going rate of pay would be for that region of the country. Could anyone direct me to a site where they might post this information? She was given a "raise" after passing the exam to $22.5/hour. I thought this was on the extreme low side.? Thanks for any input. From garreyf at gmail.com Tue May 5 18:12:49 2015 From: garreyf at gmail.com (Garreyf) Date: Tue, 5 May 2015 19:12:49 -0400 Subject: [Histonet] All Common Question 30550 In-Reply-To: <631955447A364B45B9458D2905635110D8D2953C@WIN08-MBX-01.wtbyhosp.org> References: <631955447A364B45B9458D2905635110D8D2953C@WIN08-MBX-01.wtbyhosp.org> Message-ID: Check anp.23120 tissue processing programs are validated. It's in the Ap checklist. It spells out what is required. Garrey Sent from my iPhone > On May 5, 2015, at 2:18 PM, Piche, Jessica wrote: > > Hi Everyone, > > I was just wondering if everyone has a written procedure for performance verification for their tissue processors? And if so what your procedure entails? Performance verification reports from companies that fix the tissue processor or an actual program on the tissue processor to verify that it processed tissue correctly. > > Got a ding with CAP because we didn't have a procedure in regards to the tissue processors, but I thought this question in the all common checklist had to do with chemistry analyzers and chemistry's equipment in general. Wondering what others are doing. Thank you in advance!!! > > Jessica Piche, HT(ASCP) > Waterbury Hospital > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 at comcast.net Tue May 5 18:51:39 2015 From: suetp918 at comcast.net (Sue) Date: Tue, 5 May 2015 23:51:39 +0000 (UTC) Subject: [Histonet] pay rate In-Reply-To: <1430858522736.17787@mydermsurgery.com> References: <1430858522736.17787@mydermsurgery.com> Message-ID: <1527303503.113337.1430869899703.JavaMail.zimbra@comcast.net> histo tech salaries are all over the place, hospital still do not recognize our importance and it shows with the salary range. also with re-embursements being cut it just runs down hill. sue tjuh From cbjorkhammer at sidra.org Wed May 6 00:36:44 2015 From: cbjorkhammer at sidra.org (Carl Bjorkhammer) Date: Wed, 6 May 2015 05:36:44 +0000 Subject: [Histonet] Specimen storage cabinets Message-ID: <6152F8A2AA896747A1859A03FD1E081310451A72@MV3WEXMX03PRV.smrc.sidra.org> Dear Histonet community, I?m interested in hearing your views on ducted vs filtered specimen storage cabinets. What do most people use? What are your experiences? Have you had any issues with either option? Are there any compelling evidence out there that favors one over the other? Any information is greatly appreciated! Kind regards, Nick Nick (Carl) Bj?rkhammer Medical Technologist II ? Anatomical Pathology Department of Pathology Sidra Medical and Research Center S Burj Doha, Floor 9 PO Box 26999, Doha, Qatar ??Direct: +974 4404 1842 Mobile: +974 7780 1810 ??cbjorkhammer at sidra.org www.sidra.org Save Paper - Do you really need to print this e-mail? Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. From jmoreira at sidra.org Wed May 6 01:00:39 2015 From: jmoreira at sidra.org (Joana Moreira) Date: Wed, 6 May 2015 06:00:39 +0000 Subject: [Histonet] qIHC Prep Message-ID: Anna, Diagnostic Immunohistochemistry by David J Dabbs is a great book! http://www.amazon.com/Diagnostic-Immunohistochemistry-Theranostic-Genomic-Applications/dp/1455744611 Best, Joana Joana Moreira Supervisor - Anatomical Pathology Department of Pathology Sidra Medical & Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmoreira at sidra.org | www.sidra.org Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. From blayjorge at gmail.com Wed May 6 03:10:44 2015 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Wed, 6 May 2015 04:10:44 -0400 Subject: [Histonet] Clinical Microbiology textbook recommendation Message-ID: Hello Histonet-Listers: May I have one or more recommendations re. excellent Clinical Microbiology textbooks that work well for the classroom? If you wish to answer, please send me an email off the list (blayjorge at gmail.com). Thank you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From DKBoyd at chs.net Wed May 6 06:53:01 2015 From: DKBoyd at chs.net (Boyd, Debbie M) Date: Wed, 6 May 2015 11:53:01 +0000 Subject: [Histonet] Cytology billing In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8E7F0@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8E7F0@HRHEX02-HOS.holyredeemer.local> Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9F1549C@TN001WEXMBX014.US.chs.net> #1. 88173 and 88305 #2. 88172 and 88173 Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Terri Braud [tbraud at holyredeemer.com] Sent: Tuesday, May 05, 2015 3:30 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] Cytology billing I have a question for all you cytology prep Histo folk out there. 1. If I have an FNA with 2 direct smears but NOT immediate interpretation, and an aspirate that is made into a Cell block, what will be the CPT codes charged? 2. Also, If I have an FNA with 2 direct smears for immediate interpretation, and an aspirate that will only make a thin prep, what will be the CPT codes Charged? Inquiring Histo minds need some clarity, please. Thanks, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From akbitting at geisinger.edu Wed May 6 10:36:55 2015 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Wed, 6 May 2015 15:36:55 +0000 Subject: [Histonet] Ventana dispensers Message-ID: <9358f16869934a5fae8bb9413e7bd6d7@GHSEXMBX4W12V.geisinger.edu> Good morning Histonetters, Is anyone else having issues with their Ventana detection? My dispensers are clogging up with rubbery plugs and mis-dispensing. It's not that they sit in the refrigerator and don't get used for long periods of time. We are a high volume lab and we go through detection very quickly. The biggest culprit is OptiView. I called in to log a complaint and was told to dig in the drop tubes with a pipette tip each time I use them. Really????? I've logged the complaint several times now. Just had a TAS out. She told us to do the same thing. I don't know but ever since Roche bought them, I get more and more dissatisfied. If you are having issues too, please contact me off line. Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From jpiche at wtbyhosp.org Wed May 6 11:16:22 2015 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Wed, 6 May 2015 16:16:22 +0000 Subject: [Histonet] All Common Question 30550 In-Reply-To: References: <631955447A364B45B9458D2905635110D8D2953C@WIN08-MBX-01.wtbyhosp.org> Message-ID: <631955447A364B45B9458D2905635110D8D32BEB@WIN08-MBX-01.wtbyhosp.org> Hi Garrey, The checklist question I'm referring to is actually in the All Common checklist (30550) It says the performance of all instruments and equipment is verified upon installation and after repair or reconditioning to ensure that they run according to expectations Then requires a written procedure for performance validation. There is a question regarding tissue processors in the Anatomic Path checklist 23120 regarding tissue processing programs/tissue processing programs are validated. They aren't the same question. Thanks for taking the time to reply. Jessica -----Original Message----- From: Garreyf [mailto:garreyf at gmail.com] Sent: Tuesday, May 05, 2015 7:13 PM To: Piche, Jessica Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] All Common Question 30550 Check anp.23120 tissue processing programs are validated. It's in the Ap checklist. It spells out what is required. Garrey Sent from my iPhone > On May 5, 2015, at 2:18 PM, Piche, Jessica wrote: > > Hi Everyone, > > I was just wondering if everyone has a written procedure for performance verification for their tissue processors? And if so what your procedure entails? Performance verification reports from companies that fix the tissue processor or an actual program on the tissue processor to verify that it processed tissue correctly. > > Got a ding with CAP because we didn't have a procedure in regards to the tissue processors, but I thought this question in the all common checklist had to do with chemistry analyzers and chemistry's equipment in general. Wondering what others are doing. Thank you in advance!!! > > Jessica Piche, HT(ASCP) > Waterbury Hospital > > > > CONFIDENTIALITY NOTICE: This email and any attachments contain > confidential information that is legally privileged. This information > is intended only for the use of the individual or entity named above. > The authorized recipient of this information is prohibited from > disclosing this information to any other party unless required to do > so by law or regulation. If you are not the intended recipient, you > are hereby notified that any disclosure, copying, distribution or > action taken in reliance on the contents of these documents is > strictly prohibited. If you have received this information in error, > please notify the sender immediately and delete these documents. > Copyright (c) Waterbury Hospital > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From mpence at grhs.net Wed May 6 11:21:23 2015 From: mpence at grhs.net (Mike Pence) Date: Wed, 6 May 2015 16:21:23 +0000 Subject: [Histonet] Ventana dispensers In-Reply-To: <9358f16869934a5fae8bb9413e7bd6d7@GHSEXMBX4W12V.geisinger.edu> References: <9358f16869934a5fae8bb9413e7bd6d7@GHSEXMBX4W12V.geisinger.edu> Message-ID: I know that Roche and Ventana reps monitor this site, but they may be restricted from writing on it. I hope they take these complaints seriously and don't let their company fall like so many times before when a small company is bought out by the corporate giant and they end up closing that line due to poor sales performance. -----Original Message----- From: Bitting, Angela K. [mailto:akbitting at geisinger.edu] Sent: Wednesday, May 06, 2015 11:14 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Ventana dispensers Good morning Histonetters, Is anyone else having issues with their Ventana detection? My dispensers are clogging up with rubbery plugs and mis-dispensing. It's not that they sit in the refrigerator and don't get used for long periods of time. We are a high volume lab and we go through detection very quickly. The biggest culprit is OptiView. I called in to log a complaint and was told to dig in the drop tubes with a pipette tip each time I use them. Really????? I've logged the complaint several times now. Just had a TAS out. She told us to do the same thing. I don't know but ever since Roche bought them, I get more and more dissatisfied. If you are having issues too, please contact me off line. Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aferullo at celgene.com Wed May 6 12:02:50 2015 From: aferullo at celgene.com (Andrea Ferullo) Date: Wed, 6 May 2015 17:02:50 +0000 Subject: [Histonet] Unsubscribe Message-ID: <670EC2548FAC7F4ABB9BBE40DF83120BAB145191@USSUMSPEXCMBX01.celgene.com> How can I unsubscribe? Every time I click on the link it sends me to an error page. Thanks, Andrea From amylee053 at gmail.com Mon May 4 16:51:26 2015 From: amylee053 at gmail.com (Amy Lee) Date: Mon, 4 May 2015 14:51:26 -0700 Subject: [Histonet] PAS staining Message-ID: Hello, We tried to do PAS staining on kidney. I attached my picture here. I am looking for the staining results that has more reddish as showing in the second picture. So I can do quantification better. We purchased Schiff's reagent from Sigma freshly. Could anybody teach us how can I can the staining like that? Thanks, Amy From Lacie.Algeo at providence.org Wed May 6 14:03:38 2015 From: Lacie.Algeo at providence.org (Algeo, Lacie A) Date: Wed, 6 May 2015 19:03:38 +0000 Subject: [Histonet] logging of lot numbers and expiration dates Message-ID: <24C4B3C167E5694887AB594C7602CE3A03BC6FF3@WN35104.or.providence.org> Hi All, How are people recording this information when it isn't automatically recorded in the instrumentation. For example, FS hand stain line, decalcifyer, bulk formalin..... Thank you, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo at providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From craigak12 at gmail.com Wed May 6 17:35:11 2015 From: craigak12 at gmail.com (Jb) Date: Wed, 6 May 2015 15:35:11 -0700 Subject: [Histonet] Pathology Water: Message-ID: Does anyone know what the water standard is for histology/pathology/IHC? I am trying to set up a DI system in our lab and would like to ensure quality H2O. Thank you, Craig Sent from my iPhone From linda.prasad at health.nsw.gov.au Thu May 7 00:07:11 2015 From: linda.prasad at health.nsw.gov.au (Linda Prasad (SCHN)) Date: Thu, 7 May 2015 05:07:11 +0000 Subject: [Histonet] Plasma/thrombin clot for cell block In-Reply-To: <1430842721.83074.YahooMailBasic@web161203.mail.bf1.yahoo.com> References: <1430842721.83074.YahooMailBasic@web161203.mail.bf1.yahoo.com> Message-ID: <1217DDB3D7DE5E418E3D560A268EABD0E0E8E22A@xmdb03.nch.kids> Hi Cheryl This is the technique we use. Thromboplastin Cell Block method Principle: The Thromboplastin cell block is very gentle on cells and allows a wide range of routine immunohistochemical stains to be done. Plasma, treated with EDTA, is mixed with the specimen in the presence of thromboplastin and calcium ions to form a cell clot. This method is not suitable for cells fixed in formalin or specimens of bile fluid (bile enzymes prevent clot formation) Solutions: 1. Normal Plasma, containing EDTA , use out of date plasma obtained from Blood Bank. Freeze in 2ml aliquots. This plasma has been tested for known viruses and is considered safe. 2. Thromboplastin, use out of date thromboplastin obtained from Haematology. 3. 1% calcium chloride 4. 10% formalin Method: 1. Spin fluid using a centrifuge. 2. Decant off supernatant 3. Resuspend pellet in 3 drops of plasma. 4. Add 3 drops of thromboplastin, mix 5. Add 3 drops of Calcium Chloride, mix gently 6. Allow to stand undisturbed for 15-20 minutes 7. Add 5-8ml 10% formalin and allow to fix 8. Place in a labelled tissue cassette and process as usual. Reference: Furtado (1970) Stain Technol 45(1):19-23. Thank you :) Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad at health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???Please consider the environment before printing this email. -----Original Message----- From: Cheryl [mailto:tkngflght at yahoo.com] Sent: Wednesday, 6 May 2015 2:19 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Plasma/thrombin clot for cell block HI Guys- I need procedures including products to create a plasma cell block for non-gyn cytology. The one we have uses bovine thrombin and calcium chloride (dihydrate) but it's gotten corrupted and I can't find the original resources. Help? Cheryl tkngflght at yahoo.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From ecameron1 at midcoasthealth.com Thu May 7 08:58:58 2015 From: ecameron1 at midcoasthealth.com (Cameron, Elizabeth) Date: Thu, 7 May 2015 13:58:58 +0000 Subject: [Histonet] Embedding Center Temperature Message-ID: Hi, I am curious about which temperatures people are tracking on their embedding centers for CAP, and how they are tracking them. If you are tracking the temps for the forceps and hot and cold plates, are you using the internal thermometer or measuring the temperature another way? Thanks. Elizabeth M. Cameron, HT (ASCP), QIHC Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 From Linda.Margraf at cookchildrens.org Thu May 7 09:11:37 2015 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Thu, 7 May 2015 14:11:37 +0000 Subject: [Histonet] Histonet server issues Message-ID: <928719B9EBFA1C4686918B975FF84528EE87BEBC@CCHCSMBX04.CCHCS.LDAP> Hi, I just saw the messages about there being problems with the Histonet website http://lists.utsouthwestern.edu/mailman/listinfo/histonet The university was indeed doing some work on the server at the beginning of the month and they thought it wouldn't effect users much. I checked and the website seems to be functional now and as far as I can tell, messages are going through as usual. If you have persistent problems, please let me know at the histonet-owner at lists.utsouthwestern.edu address. Thanks. Marvin Hanna's site that keeps the archive http://www.histosearch.com/histonet.html also seems to be functioning properly. Best regards, Linda M Histonet administrator From CBird at amli-denton.com Thu May 7 10:20:33 2015 From: CBird at amli-denton.com (Cindy Bird) Date: Thu, 7 May 2015 10:20:33 -0500 Subject: [Histonet] H&E Strainers Message-ID: I currently have a Sakura Tissue TEK Prisma stainer that is 10+ yrs. old and we are now having issues with staining quality. I have recently had the machine maintenance and serviced, but we are looking to purchase a new stainer . Anyone have any recommendations, or any particular instrument or vendor they wouldn't work with? Anyone work with the new Roche system that eliminates xylene and alcohol? Any feedback would be greatly appreciated. Cindy Bird From FMonson at wcupa.edu Thu May 7 11:26:40 2015 From: FMonson at wcupa.edu (Monson, Frederick) Date: Thu, 7 May 2015 16:26:40 +0000 Subject: [Histonet] Histonet links Message-ID: <2364bb9313624bfd81bd46ae7e72af76@WCUXCHP08.PASSHE.LCL> Histonet links appear to be functioning at a less-than-optimal level, but here they are anyway. I can almost 'feel the pain' afflicting Linda and Anita, but, of course, that's a pompous, maladjusted, insensitive, and politically correct lie. http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet list run by lindamargraf at gmail.com, anita.sengupta at childrens.com The above work, but I have NOT tried the emails. Just couldn't do it. If the first doesn't work, then, perhaps the 'owners' are having a problem or two. Cheers, Fred Monson P.S. The one thing that Darwinian Evolution must have is MAXIMUM variation, so get illuminated, and "B_E_E_E_E....G_O_O_O_D_D_D!!!!!" Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pennsylvania Schmucker Science South - Room SSS-024 MailDrop: Geology-Astronomy 750 South Church Street West Chester, PA, 19383 610-738-0437 fmonson at wcupa.edu From patrick.lewis at seattlechildrens.org Thu May 7 12:01:46 2015 From: patrick.lewis at seattlechildrens.org (Lewis, Patrick) Date: Thu, 7 May 2015 17:01:46 +0000 Subject: [Histonet] Still having Issues with Acetone fixation. Message-ID: <3903BE18914F4440834F0E620415FFCA3CB6D082@PPWEXD01d.childrens.sea.kids> Hi everyone, Sorry to keep posting about this, But I am still having Acetone issues. I am doing IHC for Cell surface markers that are lost when fixing with etoh or methanol. When I fix in 100% acetone my epitopes have great signal. However, when I fix in 100% acetone, my tissues all damaged by the acetone beyond all recognition. I can lose up to 90% of my tissue when I fix in 100% acetone for 10 minutes. But, I get good epitope staining if I have some tissue left on the slide. When I fix in anything else, I lose 90% or more of my epitope staining, but my tissue morphology looks great. -- What's the least amount of time I can fix in 100% Acetone for a 5uM section and still have it be fixed? Is drying after the 100% acetone fixing essential? or Bad for protecting tissue morphology? -- I am doing IHC on human tonsils cut to 5 uM with a 24 dry after sectioning. When I fix in 100% acetone, I fix it at 4C for 10 minutes. Then dry for 1 hour in the fume hood Should it go straight into buffer? Should it be for less time in the acetone? Should the acetone be Room temp or -20C instead of 4C. If I was diluting the acetone with buffer, (or etoh) then I could see going straight into buffer afterwards, but because I am using 100% I think that going into liquid right after fixing is too much of a change and my tissues go BOOM. Please help. Patrick Lewis Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From MHuth at baymedical.org Thu May 7 12:34:33 2015 From: MHuth at baymedical.org (Myra Huth) Date: Thu, 7 May 2015 17:34:33 +0000 Subject: [Histonet] Histonet Digest, Vol 138, Issue 9 In-Reply-To: Message-ID: <6B3280E0CD2C394894743E076DC66C59E248@BMCMSEXCHMB2.corp.baymedical.org> For some reason histonet is being delivered blank. -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, May 07, 2015 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 138, Issue 9 Confidentiality Notice: This email message and any attachments from Bay Medical Center Sacred Heart Health System, its subsidiaries and affiliates, are confidential and for the sole use of the intended recipient. This communication may contain privileged, proprietary, or confidential information (i.e., including Protected Health Information), which may only be used or disclosed in accordance with applicable law. If you are not the intended recipient of this email or the employee or agent responsible for delivering the communication to the intended recipient, then you may not read, copy, distribute or otherwise use or disclose the information contained in this message. If you received this message in error, please notify us by an email to Postmaster at baymedical.org. Please indicate that you were not the intended recipient, and confirm that you have deleted the original message and any attachments as well as any printed copies. Please do not retransmit the contents of the message. Thank you. From jshelley at sanfordburnham.org Thu May 7 15:33:24 2015 From: jshelley at sanfordburnham.org (John Shelley) Date: Thu, 7 May 2015 20:33:24 +0000 Subject: [Histonet] Final Week to Register for FSH Message-ID: Good Afternoon Histonetters! Hotel reservations should be made quickly to ensure rate and availability. After today you will have to hope that room prices will not go up. I am working on trying to squeeze out a few more days for the hotel registration link below. Try this link below first and if it does not work make sure that when you call (1-866-397-6516) the hotel to make reservations that you mention Florida Society for Histotechnology. Act now to save money and possible aggravation. Looking forward to seeing you at our meeting! Meeting Program/agenda http://www.fshgroup.org/wp-content/uploads/2015/03/FSH-2015-Online-Program-revised-6.pdf Hotel online reservation https://reservations.ihotelier.com/crs/g_reservation.cfm?groupID=1251402&hotelID=6579 Act quickly!!! Online meeting registration https://www.regonline.com/Register/Checkin.aspx?EventID=1679155&lbrd=1&rtypeid=380141 Prices go up after tomorrow for registration and workshops! Have a great day! Kind Regards! John Shelley 2014-2016 FSH President From Linda.Margraf at cookchildrens.org Thu May 7 15:45:51 2015 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Thu, 7 May 2015 20:45:51 +0000 Subject: [Histonet] Program in Interactive tissue microarray and quantitative digital pathology workshop announcement Message-ID: <928719B9EBFA1C4686918B975FF84528EE87C10F@CCHCSMBX04.CCHCS.LDAP> Dear Histonetters: Pasquale De Blasio asked me to provide you the announcement about a meeting in Rome this June. I copied the content from the meeting flyer below. Unfortunately, the Histonet server would block all the graphics and artwork in the flyer so I could not include that. I have no personal knowledge of him or the meeting. His email is pasquale.deblasio at isenet.it if you have questions about this meeting. Thanks. PRELIMINARY PROGRAM INTERACTIVE TISSUE MICROARRAY AND QUANTITATIVE DIGITAL PATHOLOGY WORKSHOP June 8-9th 2015 University of "Tor Vergata", Campus X, Rome - Italy On-line registration: https://eventbrite.com/event/16536197211 June 8th, 2015 08:00 - 08:45 REGISTRATION 08:45 - 09:00 WELCOME Remarks - Prof. Luigi Spagnoli, Pasquale De Blasio 09:00 - 11:00 SESSION 1 - TISSUE MICROARRAY TECHNOLOGY - Chair: Pasquale De Blasio - History of Tissue Microarray Technology Ulrich F. Vogel, UKT, Institute of Pathology, Tuebingen, (Germany) - Visualizing and Quantifying Cell Populations from Brightfield and Fluorescent TMA Samples Thomas J. Diefenbach, The Ragon Institute of MGH, MIT and Harvard - Cambridge, MA (USA) - Management of a Pathology TMA and Virtual Image Infrastructure for Research and Diagnostics Peter Riegman, Erasmus MC Cancer Institute, Rotterdam, (The Netherlands) - TMA Technology for: Tissue Microdissection, DNA & RNA and protein extract Giorgio Stanta, Department of Medical Sciences, University of Trieste, (Italy) 11:00 - 11:20 Coffee break 11:20 - 13:30 SESSION 2 - PRACTICAL CONSTRUCTION OF A TISSUE ARRAY - Organization of FFPE Archive for TMA Use in Research and Diagnostic Luigi Spagnoli, Professor of Pathology Emeritus, University of Tor Vergata, Rome (Italy) - Tissue and Cell Microarray: A Cross-Over Validation Tool for Stem Cell Research Ida Biunno, Institute of Genetic and Biomedical Research (IRGB-CNR) , Milan (Italy) - Concepts of Quality Assurance and Quality Control in the use of Tissues Paolo Locatelli, Area Manager, Milestone srl, Sorisole - Bergamo (Italy) - TMA Application in Neurodegenerative Disorders Roberto Dominici, Analysis and Diagnostic Laboratory, Abbiategrasso Hospital, Milan (Italy) - Ethical Aspects of use of TMA slides for Research and Diagnostics Speaker to be confirmed 13:30 - 14:30 Lunch Break 14:00 - 17:00 SESSION 3 - HANDS-ON TISSUE ARRAYERS, SCANNERS AND IMAGE ANALYSIS SYSTEMS - Tissue Microarrayer Platform Bring your own Tissue Blocks and make your TMA - Digital Scanners & Image Analysis Software (Analyze your TMA Slide) Bring your Tissue and TMA Slide and get them analysed - Visit Exhibition Boots and look at instruments and tools which can enhance your work Tissue Vacuum technology, Digital Scanners, Visual Imaging Software June 9th, 2015 09:00 - 11:00 SESSION 4 - QUANTITATIVE DIGITAL PATHOLOGY IMAGE ANALYSIS - TMA Platform for Biomarkers Validation and Clinical Applications Pasquale De Blasio, Integrated Systems Engineering srl, Milan (Italy) - Automated image analysis in histopathology: a valuable tool in medical diagnostics George Steiner, TissueGnostics, Wien (Austria) - Digital pathology: combining whole slide imaging, multiplex staining and automated image analysis Speaker to be confirmed (Visiopharm) - Proliferation markers and automated tumour detection Speaker to be confirmed (Hamamatsu) 11:30 - 13:00 SESSION 4 - (Cont.) QUANTITATIVE DIGITAL PATHOLOGY IMAGE ANALYSIS - Review of imaging solutions for integrated quantitative immunohistochemistry in the Pathology daily practice Speaker to be confirmed (Leika/Aperio) - Digital pathology: current status and future perspectives Speaker to be confirmed (Dako) - Virtual microscopy and digital pathology in training and education Speaker to be confirmed (PathXL) - Virtual Microscope System Speaker to be confirmed (Olympus) 13:00 - 14:00 Lunch 14:00 - 16:00 SESSION 5 - HANDS-ON TISSUE ARRAYERS, SCANNERS AND IMAGE ANALYSIS SYSTEMS - Tissue Microarrayer Platform Bring your own Tissue Blocks and make your TMA - Digital Scanners & Image Analysis Software (Analyze your TMA Slide) Bring your Tissue and TMA Slide and get them analysed - Visit Exhibition Boots and look at instruments and tools which can enhance your work Tissue Vacuum technology, Digital Scanners, Visual Imaging From jvickroy at SpringfieldClinic.com Thu May 7 15:57:45 2015 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Thu, 7 May 2015 20:57:45 +0000 Subject: [Histonet] Billing question Message-ID: <9B1A1501A800064397369BD8072E6BCAB718ED@E2K10DB.springfieldclinic.com> If I have two sections on an "A" specimen A1 and A2 and both had a GMS stain, do I charge 1 - 88312 or 2 - 88312? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From Joyce.Weems at emoryhealthcare.org Thu May 7 16:15:45 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Thu, 7 May 2015 21:15:45 +0000 Subject: [Histonet] Billing question In-Reply-To: <9B1A1501A800064397369BD8072E6BCAB718ED@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCAB718ED@E2K10DB.springfieldclinic.com> Message-ID: 2 - specials are per block.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Vickroy, James [mailto:jvickroy at SpringfieldClinic.com] Sent: Thursday, May 07, 2015 4:58 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Billing question If I have two sections on an "A" specimen A1 and A2 and both had a GMS stain, do I charge 1 - 88312 or 2 - 88312? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From JMacDonald at mtsac.edu Thu May 7 17:07:59 2015 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Thu, 7 May 2015 15:07:59 -0700 Subject: [Histonet] HT jobs in Eureka, CA Message-ID: I have a graduate looking for histotechnician jobs in the Eureka, CA area. Please contact me if you know of any. Thanks, Jennifer MacDonald From tmcampbe at fmh.org Fri May 8 06:50:52 2015 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Fri, 8 May 2015 11:50:52 +0000 Subject: [Histonet] Billing question In-Reply-To: <9B1A1501A800064397369BD8072E6BCAB718ED@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCAB718ED@E2K10DB.springfieldclinic.com> Message-ID: >From my understanding its just one charge. Its per specimen such as A, B, C, etc. Someone else said that it would be 2 charges so am I wrong on this?? If you did GMS on A1 and AFB on A2 then that would be 2 charges because its two different stains. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: Vickroy, James [mailto:jvickroy at SpringfieldClinic.com] Sent: Thursday, May 07, 2015 4:58 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Billing question If I have two sections on an "A" specimen A1 and A2 and both had a GMS stain, do I charge 1 - 88312 or 2 - 88312? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anna.coffey at nih.gov Fri May 8 09:50:42 2015 From: anna.coffey at nih.gov (Coffey, Anna (NIH/NCI) [C]) Date: Fri, 8 May 2015 14:50:42 +0000 Subject: [Histonet] B-gal positive control Message-ID: <5C3E10119A1B824FBE92B08279F74A91017995AF@msgb10.nih.gov> Hello Histonet, Has anyone out there come across a good FFPE positive control for B-gal? If so, please let me know! We would like to purchase a block or unstained slides if at all possible. Thanks! Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey at nih.gov 301-846-1730 From dellav at musc.edu Fri May 8 10:51:18 2015 From: dellav at musc.edu (della Speranza, Vinnie) Date: Fri, 8 May 2015 15:51:18 +0000 Subject: [Histonet] Billing question In-Reply-To: References: <9B1A1501A800064397369BD8072E6BCAB718ED@E2K10DB.springfieldclinic.com> Message-ID: This is incorrect. The response from Joyce Weems is correct. And yes, it's confusing to everyone. Special stains are billed per block IHC is billed per specimen So a GMS on blocks A1 and A2 is GMS charge x 2 If you are not charging special stains per block you are losing revenue you are entitled to for 2015 Vinnie Della Speranza | Manager for Anatomic Pathology Services| Medical University of South Carolina | 165 Ashley Avenue MSC 908 | Charleston, South Carolina 29425 | Office: 843.792.6353 | Fax: 843.792.8974 | dellav at musc.edu -----Original Message----- From: Campbell, Tasha M. [mailto:tmcampbe at fmh.org] Sent: Friday, May 08, 2015 7:51 AM To: Vickroy, James; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Billing question >From my understanding its just one charge. Its per specimen such as A, B, C, etc. Someone else said that it would be 2 charges so am I wrong on this?? If you did GMS on A1 and AFB on A2 then that would be 2 charges because its two different stains. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: Vickroy, James [mailto:jvickroy at SpringfieldClinic.com] Sent: Thursday, May 07, 2015 4:58 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Billing question If I have two sections on an "A" specimen A1 and A2 and both had a GMS stain, do I charge 1 - 88312 or 2 - 88312? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr at comcast.net Fri May 8 11:04:53 2015 From: koellingr at comcast.net (koellingr at comcast.net) Date: Fri, 8 May 2015 16:04:53 +0000 (UTC) Subject: [Histonet] B-gal positive control In-Reply-To: <5C3E10119A1B824FBE92B08279F74A91017995AF@msgb10.nih.gov> References: <5C3E10119A1B824FBE92B08279F74A91017995AF@msgb10.nih.gov> Message-ID: <1827913949.5910894.1431101093875.JavaMail.zimbra@comcast.net> Anna, Don't know if you are talking human or non-human control?or if makes a difference and I don't want to get into that whole discussion again. But if a mouse control is OK, one of the cleanest and nicest systems I used? for B-gal was to get a transgenic mouse, easily obtainable with a Tie-2/lacZ promoter/reporter.? B-gal expressed only on vascular endothelium so your assay can easily be tweaked for strength and cleanliness of signal.? Have all the frozen/FFPE blocks you could ever need. Ray, Lake Forest Park, WA ----- Original Message ----- From: "Anna Coffey (NIH/NCI) [C]" To: histonet at lists.utsouthwestern.edu Sent: Friday, May 8, 2015 7:50:42 AM Subject: [Histonet] B-gal positive control Hello Histonet, Has anyone out there come across a good FFPE positive control for B-gal? If so, please let me know! We would like to purchase a block or unstained slides if at all possible. Thanks! Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey at nih.gov 301-846-1730 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems at emoryhealthcare.org Fri May 8 11:16:54 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Fri, 8 May 2015 16:16:54 +0000 Subject: [Histonet] Billing question In-Reply-To: References: <9B1A1501A800064397369BD8072E6BCAB718ED@E2K10DB.springfieldclinic.com> Message-ID: Special stains are charged per block. If you have blocks A1, A2, and A3, and do AFB and GMS on all three that would be 6 charges. Shhhhh... this will probably change next year!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Campbell, Tasha M. [mailto:tmcampbe at fmh.org] Sent: Friday, May 08, 2015 7:51 AM To: Vickroy, James; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Billing question >From my understanding its just one charge. Its per specimen such as A, B, C, etc. Someone else said that it would be 2 charges so am I wrong on this?? If you did GMS on A1 and AFB on A2 then that would be 2 charges because its two different stains. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -----Original Message----- From: Vickroy, James [mailto:jvickroy at SpringfieldClinic.com] Sent: Thursday, May 07, 2015 4:58 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Billing question If I have two sections on an "A" specimen A1 and A2 and both had a GMS stain, do I charge 1 - 88312 or 2 - 88312? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From mike at pathview.com Fri May 8 11:41:18 2015 From: mike at pathview.com (Michael Mihalik) Date: Fri, 8 May 2015 09:41:18 -0700 Subject: [Histonet] Friday Trivia Question: Most specimen on a single case Message-ID: <012901d089ad$cfd8e4f0$6f8aaed0$@pathview.com> Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. I'm curious how common this is. What's the most specimen on a single case you've ever seen? Thanks for your patience and experience. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 From Stacy_McLaughlin at cooley-dickinson.org Fri May 8 11:48:31 2015 From: Stacy_McLaughlin at cooley-dickinson.org (Stacy McLaughlin) Date: Fri, 8 May 2015 16:48:31 +0000 Subject: [Histonet] Friday Trivia Question: Most specimen on a single case In-Reply-To: <012901d089ad$cfd8e4f0$6f8aaed0$@pathview.com> References: <012901d089ad$cfd8e4f0$6f8aaed0$@pathview.com> Message-ID: I've never seen that many on one case. The most I've seen is ~30 (parathyroid) and its been many years. What types of specimens are they? Stacy -----Original Message----- From: Michael Mihalik [mailto:mike at pathview.com] Sent: Friday, May 08, 2015 12:41 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Friday Trivia Question: Most specimen on a single case Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. I'm curious how common this is. What's the most specimen on a single case you've ever seen? Thanks for your patience and experience. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum at uams.edu Fri May 8 11:50:24 2015 From: PAMarcum at uams.edu (Marcum, Pamela A) Date: Fri, 8 May 2015 16:50:24 +0000 Subject: [Histonet] Friday Trivia Question: Most specimen on a single case In-Reply-To: <012901d089ad$cfd8e4f0$6f8aaed0$@pathview.com> References: <012901d089ad$cfd8e4f0$6f8aaed0$@pathview.com> Message-ID: 157 blocks on one breast case - resident grossed it. -----Original Message----- From: Michael Mihalik [mailto:mike at pathview.com] Sent: Friday, May 08, 2015 11:41 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Friday Trivia Question: Most specimen on a single case Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. I'm curious how common this is. What's the most specimen on a single case you've ever seen? Thanks for your patience and experience. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From bcooper at chla.usc.edu Fri May 8 11:51:29 2015 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Fri, 8 May 2015 16:51:29 +0000 Subject: [Histonet] Friday Trivia Question: Most specimen on a single case In-Reply-To: References: <012901d089ad$cfd8e4f0$6f8aaed0$@pathview.com> Message-ID: I once saw an entire breast submitted--it was something like 100+ blocks. Thanks, Brian -----Original Message----- From: Stacy McLaughlin [mailto:Stacy_McLaughlin at cooley-dickinson.org] Sent: Friday, May 08, 2015 9:49 AM To: Michael Mihalik; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Friday Trivia Question: Most specimen on a single case I've never seen that many on one case. The most I've seen is ~30 (parathyroid) and its been many years. What types of specimens are they? Stacy -----Original Message----- From: Michael Mihalik [mailto:mike at pathview.com] Sent: Friday, May 08, 2015 12:41 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Friday Trivia Question: Most specimen on a single case Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. I'm curious how common this is. What's the most specimen on a single case you've ever seen? Thanks for your patience and experience. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From Timothy.Morken at ucsf.edu Fri May 8 11:52:42 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 8 May 2015 16:52:42 +0000 Subject: [Histonet] Friday Trivia Question: Most specimen on a single case In-Reply-To: <012901d089ad$cfd8e4f0$6f8aaed0$@pathview.com> References: <012901d089ad$cfd8e4f0$6f8aaed0$@pathview.com> Message-ID: <761E2B5697F795489C8710BCC72141FF36830914@ex07.net.ucsf.edu> Blocks, yes. Parts of a case (specimens), no. -----Original Message----- From: Michael Mihalik [mailto:mike at pathview.com] Sent: Friday, May 08, 2015 9:41 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Friday Trivia Question: Most specimen on a single case Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. I'm curious how common this is. What's the most specimen on a single case you've ever seen? Thanks for your patience and experience. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV at archildrens.org Fri May 8 12:13:28 2015 From: HornHV at archildrens.org (Horn, Hazel V) Date: Fri, 8 May 2015 12:13:28 -0500 Subject: [Histonet] Friday Trivia Question: Most specimen on a single case In-Reply-To: <012901d089ad$cfd8e4f0$6f8aaed0$@pathview.com> References: <012901d089ad$cfd8e4f0$6f8aaed0$@pathview.com> Message-ID: <25A4DE08332B19499904459F00AAACB71A1EB25562@EVS1.archildrens.org> 227 blocks on a bone tumor. Resident grossed it of couse! It was a nightmare. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org -----Original Message----- From: Michael Mihalik [mailto:mike at pathview.com] Sent: Friday, May 08, 2015 11:41 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Friday Trivia Question: Most specimen on a single case Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. I'm curious how common this is. What's the most specimen on a single case you've ever seen? Thanks for your patience and experience. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From mike at pathview.com Fri May 8 12:34:29 2015 From: mike at pathview.com (Michael Mihalik) Date: Fri, 8 May 2015 10:34:29 -0700 Subject: [Histonet] Friday Trivia Question: Most specimen on a single case In-Reply-To: <133C8EDB80237E41B9CED3DF5C4FDD101D6BDF2F@ugunhpsj.easf.csd.disa.mil> References: <012901d089ad$cfd8e4f0$6f8aaed0$@pathview.com> <133C8EDB80237E41B9CED3DF5C4FDD101D6BDF2F@ugunhpsj.easf.csd.disa.mil> Message-ID: <018201d089b5$3ddec580$b99c5080$@pathview.com> I did ask about specimen/container, but knowing block count is interesting as well. Guys, I'm just the LIS vendor and I'm just happy that our LIS can handle this. Since it's been asked, I have asked what 'kind' of specimen these are. Oh, and as far as billing is concerned, since this is hospital based, I believe the billing will based upon admitting icd code... Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: Young, Henry O III CIV USN NAVHOSP CLNC (US) [mailto:henry.o.young.civ at mail.mil] Sent: Friday, May 08, 2015 10:19 AM To: Marcum, Pamela A; 'Michael Mihalik'; histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Trivia Question: Most specimen on a single case Read the question, it is a 100 containers on one case. Not block count. HY -----Original Message----- From: Marcum, Pamela A [mailto:PAMarcum at uams.edu] Sent: Friday, May 08, 2015 12:50 PM To: 'Michael Mihalik'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Friday Trivia Question: Most specimen on a single case 157 blocks on one breast case - resident grossed it. -----Original Message----- From: Michael Mihalik [mailto:mike at pathview.com] Sent: Friday, May 08, 2015 11:41 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Friday Trivia Question: Most specimen on a single case Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. I'm curious how common this is. What's the most specimen on a single case you've ever seen? Thanks for your patience and experience. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From badams at acadianagastro.com Fri May 8 13:48:36 2015 From: badams at acadianagastro.com (Brent Adams) Date: Fri, 8 May 2015 18:48:36 +0000 Subject: [Histonet] Histology Lab Assistant / Medical Assistant Message-ID: <1431110916505.21971@acadianagastro.com> Acadiana Gastroenterology Associates' of Lafayette Louisiana is looking for a Part-time (25-30 hour/ week) Histology Lab Assistant for our small GI Laboratory. Hours are flexible and Monday thru Friday only. The Ideal candidate would have previous Laboratory Assistant experience in an Anatomical Pathology Laboratory. This should include working with reagents and chemicals, filing blocks, working with staining and coverslip equipment. Strong knowledge of Safety Regulations and OSHA Standards. Must have a good understanding of Medical Terminology and must have High School diploma or GED equivalent and must be legally employable in the state of Louisiana. Apply on line at careerbuilders.com. Brent Adams - BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. From mprice63 at live.com Fri May 8 16:14:57 2015 From: mprice63 at live.com (Marsha Price) Date: Fri, 8 May 2015 16:14:57 -0500 Subject: [Histonet] Short Term travel Tech Agencies In-Reply-To: References: Message-ID: Does anyone know of an agency that hires Histology Techs for short term assignments, less than 3 month assignments? Marsha Price Sent from my iPad > On May 8, 2015, at 12:00 PM, histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Still having Issues with Acetone fixation. (Lewis, Patrick) > 2. Re: Histonet Digest, Vol 138, Issue 9 (Myra Huth) > 3. Final Week to Register for FSH (John Shelley) > 4. Program in Interactive tissue microarray and quantitative > digital pathology workshop announcement (Linda Margraf) > 5. Billing question (Vickroy, James) > 6. Re: Billing question (Weems, Joyce K.) > 7. HT jobs in Eureka, CA (Jennifer MacDonald) > 8. Re: Billing question (Campbell, Tasha M.) > 9. B-gal positive control (Coffey, Anna (NIH/NCI) [C]) > 10. Re: Billing question (della Speranza, Vinnie) > 11. Re: B-gal positive control (koellingr at comcast.net) > 12. Re: Billing question (Weems, Joyce K.) > 13. Friday Trivia Question: Most specimen on a single case > (Michael Mihalik) > 14. Re: Friday Trivia Question: Most specimen on a single case > (Stacy McLaughlin) > 15. Re: Friday Trivia Question: Most specimen on a single case > (Marcum, Pamela A) > 16. Re: Friday Trivia Question: Most specimen on a single case > (Cooper, Brian) > 17. Re: Friday Trivia Question: Most specimen on a single case > (Morken, Timothy) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 7 May 2015 17:01:46 +0000 > From: "Lewis, Patrick" > To: " (Histonet at lists.utsouthwestern.edu)" > , 16S-CSF Study > <16S-CSFStudy at seattlechildrens.org> > Subject: [Histonet] Still having Issues with Acetone fixation. > Message-ID: > <3903BE18914F4440834F0E620415FFCA3CB6D082 at PPWEXD01d.childrens.sea.kids> > > Content-Type: text/plain; charset="us-ascii" > > Hi everyone, > > Sorry to keep posting about this, > > But I am still having Acetone issues. > > I am doing IHC for Cell surface markers that are lost when fixing with etoh or methanol. > > When I fix in 100% acetone my epitopes have great signal. > > However, when I fix in 100% acetone, my tissues all damaged by the acetone beyond all recognition. > > I can lose up to 90% of my tissue when I fix in 100% acetone for 10 minutes. > > But, I get good epitope staining if I have some tissue left on the slide. > > When I fix in anything else, I lose 90% or more of my epitope staining, but my tissue morphology looks great. > > -- > What's the least amount of time I can fix in 100% Acetone for a 5uM section and still have it be fixed? > > Is drying after the 100% acetone fixing essential? or Bad for protecting tissue morphology? > > -- > I am doing IHC on human tonsils cut to 5 uM with a 24 dry after sectioning. > > When I fix in 100% acetone, I fix it at 4C for 10 minutes. Then dry for 1 hour in the fume hood > > Should it go straight into buffer? Should it be for less time in the acetone? > Should the acetone be Room temp or -20C instead of 4C. > > If I was diluting the acetone with buffer, (or etoh) then I could see going straight into buffer afterwards, but because I am using 100% I think that going into liquid right after fixing is too much of a change and my tissues go BOOM. > > Please help. > > Patrick Lewis > > > Patrick Lewis > Research Associate II Bench > Seattle Childrens Research Institute > 206-884-1115 > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > > ------------------------------ > > Message: 2 > Date: Thu, 7 May 2015 17:34:33 +0000 > From: Myra Huth > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: Re: [Histonet] Histonet Digest, Vol 138, Issue 9 > Message-ID: > <6B3280E0CD2C394894743E076DC66C59E248 at BMCMSEXCHMB2.corp.baymedical.org> > > Content-Type: text/plain; charset="us-ascii" > > For some reason histonet is being delivered blank. > > -----Original Message----- > From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] > Sent: Thursday, May 07, 2015 12:00 PM > To: histonet at lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 138, Issue 9 > > > Confidentiality Notice: This email message and any attachments from Bay Medical Center Sacred Heart Health System, its subsidiaries and affiliates, are confidential and for the sole use of the intended recipient. This communication may contain privileged, proprietary, or confidential information (i.e., including Protected Health Information), which may only be used or disclosed in accordance with applicable law. If you are not the intended recipient of this email or the employee or agent responsible for delivering the communication to the intended recipient, then you may not read, copy, distribute or otherwise use or disclose the information contained in this message. If you received this message in error, please notify us by an email to Postmaster at baymedical.org. Please indicate that you were not the intended recipient, and confirm that you have deleted the original message and any attachments as well as any printed copies. Please do not retransmit the contents of the message. Thank you. > > > ------------------------------ > > Message: 3 > Date: Thu, 7 May 2015 20:33:24 +0000 > From: John Shelley > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Final Week to Register for FSH > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Good Afternoon Histonetters! > > Hotel reservations should be made quickly to ensure rate and availability. After today you will have to hope that room prices will not go up. I am working on trying to squeeze out a few more days for the hotel registration link below. Try this link below first and if it does not work make sure that when you call (1-866-397-6516) the hotel to make reservations that you mention Florida Society for Histotechnology. Act now to save money and possible aggravation. Looking forward to seeing you at our meeting! > > Meeting Program/agenda http://www.fshgroup.org/wp-content/uploads/2015/03/FSH-2015-Online-Program-revised-6.pdf > > Hotel online reservation https://reservations.ihotelier.com/crs/g_reservation.cfm?groupID=1251402&hotelID=6579 Act quickly!!! > > Online meeting registration https://www.regonline.com/Register/Checkin.aspx?EventID=1679155&lbrd=1&rtypeid=380141 Prices go up after tomorrow for registration and workshops! > > Have a great day! > > Kind Regards! > > John Shelley > 2014-2016 FSH President > > > > ------------------------------ > > Message: 4 > Date: Thu, 7 May 2015 20:45:51 +0000 > From: Linda Margraf > To: "'histonet at lists.utsouthwestern.edu'" > > Cc: "'pasquale.deblasio at isenet.it'" > Subject: [Histonet] Program in Interactive tissue microarray and > quantitative digital pathology workshop announcement > Message-ID: > <928719B9EBFA1C4686918B975FF84528EE87C10F at CCHCSMBX04.CCHCS.LDAP> > Content-Type: text/plain; charset="us-ascii" > > Dear Histonetters: > > > > Pasquale De Blasio asked me to provide you the announcement about a meeting in Rome this June. I copied the content from the meeting flyer below. Unfortunately, the Histonet server would block all the graphics and artwork in the flyer so I could not include that. I have no personal knowledge of him or the meeting. His email is pasquale.deblasio at isenet.it if you have questions about this meeting. Thanks. > > > > > > > > PRELIMINARY PROGRAM INTERACTIVE TISSUE MICROARRAY AND QUANTITATIVE DIGITAL PATHOLOGY WORKSHOP > > June 8-9th 2015 University of "Tor Vergata", Campus X, Rome - Italy > > On-line registration: https://eventbrite.com/event/16536197211 > > > > June 8th, 2015 > > 08:00 - 08:45 REGISTRATION > > 08:45 - 09:00 WELCOME Remarks - Prof. Luigi Spagnoli, Pasquale De Blasio > > 09:00 - 11:00 SESSION 1 - TISSUE MICROARRAY TECHNOLOGY - Chair: Pasquale De Blasio > - History of Tissue Microarray Technology > Ulrich F. Vogel, UKT, Institute of Pathology, Tuebingen, (Germany) > - Visualizing and Quantifying Cell Populations from Brightfield and Fluorescent TMA Samples > Thomas J. Diefenbach, The Ragon Institute of MGH, MIT and Harvard - Cambridge, MA (USA) > - Management of a Pathology TMA and Virtual Image Infrastructure for Research and Diagnostics > Peter Riegman, Erasmus MC Cancer Institute, Rotterdam, (The Netherlands) > > - TMA Technology for: Tissue Microdissection, DNA & RNA and protein extract > Giorgio Stanta, Department of Medical Sciences, University of Trieste, (Italy) > > 11:00 - 11:20 Coffee break > > 11:20 - 13:30 SESSION 2 - PRACTICAL CONSTRUCTION OF A TISSUE ARRAY > - Organization of FFPE Archive for TMA Use in Research and Diagnostic > Luigi Spagnoli, Professor of Pathology Emeritus, University of Tor Vergata, Rome (Italy) > - Tissue and Cell Microarray: A Cross-Over Validation Tool for Stem Cell Research > Ida Biunno, Institute of Genetic and Biomedical Research (IRGB-CNR) , Milan (Italy) > - Concepts of Quality Assurance and Quality Control in the use of Tissues > Paolo Locatelli, Area Manager, Milestone srl, Sorisole - Bergamo (Italy) > - TMA Application in Neurodegenerative Disorders > Roberto Dominici, Analysis and Diagnostic Laboratory, Abbiategrasso Hospital, Milan (Italy) > - Ethical Aspects of use of TMA slides for Research and Diagnostics > Speaker to be confirmed > > 13:30 - 14:30 Lunch Break > > 14:00 - 17:00 SESSION 3 - HANDS-ON TISSUE ARRAYERS, SCANNERS AND IMAGE ANALYSIS SYSTEMS > - Tissue Microarrayer Platform > Bring your own Tissue Blocks and make your TMA > - Digital Scanners & Image Analysis Software (Analyze your TMA Slide) > Bring your Tissue and TMA Slide and get them analysed > - Visit Exhibition Boots and look at instruments and tools which can enhance your work > Tissue Vacuum technology, Digital Scanners, Visual Imaging Software > > > > June 9th, 2015 > > 09:00 - 11:00 SESSION 4 - QUANTITATIVE DIGITAL PATHOLOGY IMAGE ANALYSIS > > - TMA Platform for Biomarkers Validation and Clinical Applications > > Pasquale De Blasio, Integrated Systems Engineering srl, Milan (Italy) > > - Automated image analysis in histopathology: a valuable tool in medical diagnostics > > George Steiner, TissueGnostics, Wien (Austria) > > - Digital pathology: combining whole slide imaging, multiplex staining and automated image analysis > > Speaker to be confirmed (Visiopharm) > > - Proliferation markers and automated tumour detection > > Speaker to be confirmed (Hamamatsu) > > > > 11:30 - 13:00 SESSION 4 - (Cont.) QUANTITATIVE DIGITAL PATHOLOGY IMAGE ANALYSIS > > - Review of imaging solutions for integrated quantitative immunohistochemistry in the Pathology daily practice > > Speaker to be confirmed (Leika/Aperio) > > - Digital pathology: current status and future perspectives > > Speaker to be confirmed (Dako) > > - Virtual microscopy and digital pathology in training and education > > Speaker to be confirmed (PathXL) > > - Virtual Microscope System > > Speaker to be confirmed (Olympus) > > 13:00 - 14:00 Lunch > > > > 14:00 - 16:00 SESSION 5 - HANDS-ON TISSUE ARRAYERS, SCANNERS AND IMAGE ANALYSIS SYSTEMS > > - Tissue Microarrayer Platform > > Bring your own Tissue Blocks and make your TMA > > - Digital Scanners & Image Analysis Software (Analyze your TMA Slide) > > Bring your Tissue and TMA Slide and get them analysed > > - Visit Exhibition Boots and look at instruments and tools which can enhance your work > Tissue Vacuum technology, Digital Scanners, Visual Imaging > > > > ------------------------------ > > Message: 5 > Date: Thu, 7 May 2015 20:57:45 +0000 > From: "Vickroy, James" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Billing question > Message-ID: > <9B1A1501A800064397369BD8072E6BCAB718ED at E2K10DB.springfieldclinic.com> > Content-Type: text/plain; charset="us-ascii" > > If I have two sections on an "A" specimen A1 and A2 and both had a GMS stain, do I charge 1 - 88312 or 2 - 88312? > > Jim > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvickroy at SpringfieldClinic.com > > > > This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. > > ------------------------------ > > Message: 6 > Date: Thu, 7 May 2015 21:15:45 +0000 > From: "Weems, Joyce K." > To: "'Vickroy, James'" , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Billing question > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > 2 - specials are per block.. > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems at emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > -----Original Message----- > From: Vickroy, James [mailto:jvickroy at SpringfieldClinic.com] > Sent: Thursday, May 07, 2015 4:58 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Billing question > > If I have two sections on an "A" specimen A1 and A2 and both had a GMS stain, do I charge 1 - 88312 or 2 - 88312? > > Jim > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvickroy at SpringfieldClinic.com > > > > This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > > > > ------------------------------ > > Message: 7 > Date: Thu, 7 May 2015 15:07:59 -0700 > From: Jennifer MacDonald > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] HT jobs in Eureka, CA > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > I have a graduate looking for histotechnician jobs in the Eureka, CA area. > Please contact me if you know of any. Thanks, > Jennifer MacDonald > > ------------------------------ > > Message: 8 > Date: Fri, 8 May 2015 11:50:52 +0000 > From: "Campbell, Tasha M." > To: "Vickroy, James" , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Billing question > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > >> From my understanding its just one charge. Its per specimen such as A, B, C, etc. Someone else said that it would be 2 charges so am I wrong on this?? If you did GMS on A1 and AFB on A2 then that would be 2 charges because its two different stains. > > > > > Tasha Campbell, B.S.,HTL(ASCP) > Frederick Gastroenterology Associates > 310 W. 9th St. > Frederick, MD 21701 > 301-695-6800 ext. 144 (w) > 304-685-9307 (c) > > -----Original Message----- > From: Vickroy, James [mailto:jvickroy at SpringfieldClinic.com] > Sent: Thursday, May 07, 2015 4:58 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Billing question > > If I have two sections on an "A" specimen A1 and A2 and both had a GMS stain, do I charge 1 - 88312 or 2 - 88312? > > Jim > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvickroy at SpringfieldClinic.com > > > > This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Fri, 8 May 2015 14:50:42 +0000 > From: "Coffey, Anna (NIH/NCI) [C]" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] B-gal positive control > Message-ID: <5C3E10119A1B824FBE92B08279F74A91017995AF at msgb10.nih.gov> > Content-Type: text/plain; charset="us-ascii" > > Hello Histonet, > > Has anyone out there come across a good FFPE positive control for B-gal? If so, please let me know! We would like to purchase a block or unstained slides if at all possible. > > Thanks! > Anna > > Anna Coffey, MS, HTL(ASCP)CM > Histotechnologist > Center for Advanced Preclinical Research > Frederick National Laboratory for Cancer Research > Leidos Biomedical Research, Inc. > Bld 539, 224 > Frederick, Maryland 21702 > anna.coffey at nih.gov > 301-846-1730 > > > > ------------------------------ > > Message: 10 > Date: Fri, 8 May 2015 15:51:18 +0000 > From: "della Speranza, Vinnie" > To: "Campbell, Tasha M." , "Vickroy, James" > , "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Billing question > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > This is incorrect. The response from Joyce Weems is correct. > > And yes, it's confusing to everyone. > > > > Special stains are billed per block > > IHC is billed per specimen > > > > So a GMS on blocks A1 and A2 is GMS charge x 2 > > > > If you are not charging special stains per block you are losing revenue you are entitled to for 2015 > > > > Vinnie Della Speranza | Manager for Anatomic Pathology Services| Medical University of South Carolina | 165 Ashley Avenue MSC 908 | Charleston, South Carolina 29425 | Office: 843.792.6353 | Fax: 843.792.8974 | dellav at musc.edu > > > > > > > > > > -----Original Message----- > From: Campbell, Tasha M. [mailto:tmcampbe at fmh.org] > Sent: Friday, May 08, 2015 7:51 AM > To: Vickroy, James; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Billing question > > > > > > > >> From my understanding its just one charge. Its per specimen such as A, B, C, etc. Someone else said that it would be 2 charges so am I wrong on this?? If you did GMS on A1 and AFB on A2 then that would be 2 charges because its two different stains. > > > > > > > > > > Tasha Campbell, B.S.,HTL(ASCP) > > Frederick Gastroenterology Associates > > 310 W. 9th St. > > Frederick, MD 21701 > > 301-695-6800 ext. 144 (w) > > 304-685-9307 (c) > > > > -----Original Message----- > > From: Vickroy, James [mailto:jvickroy at SpringfieldClinic.com] > > Sent: Thursday, May 07, 2015 4:58 PM > > To: histonet at lists.utsouthwestern.edu > > Subject: [Histonet] Billing question > > > > If I have two sections on an "A" specimen A1 and A2 and both had a GMS stain, do I charge 1 - 88312 or 2 - 88312? > > > > Jim > > > > Jim Vickroy > > Histology Manager > > Springfield Clinic, Main Campus, East Building > > 1025 South 6th Street > > Springfield, Illinois 62703 > > Office: 217-528-7541, Ext. 15121 > > Email: jvickroy at SpringfieldClinic.com> > > > > > > > > This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 11 > Date: Fri, 8 May 2015 16:04:53 +0000 (UTC) > From: koellingr at comcast.net > To: "Anna Coffey (NIH/NCI) [C]" > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] B-gal positive control > Message-ID: > <1827913949.5910894.1431101093875.JavaMail.zimbra at comcast.net> > Content-Type: text/plain; charset=utf-8 > > Anna, > Don't know if you are talking human or non-human control?or if makes a difference and I don't want to get into that whole discussion again. But if a mouse control is OK, one of the cleanest and nicest systems I used? for B-gal was to get a transgenic mouse, easily obtainable with a Tie-2/lacZ promoter/reporter.? B-gal expressed only on vascular endothelium so your assay can easily be tweaked for strength and cleanliness of signal.? Have all the frozen/FFPE blocks you could ever need. > Ray, Lake Forest Park, WA > > ----- Original Message ----- > > From: "Anna Coffey (NIH/NCI) [C]" > To: histonet at lists.utsouthwestern.edu > Sent: Friday, May 8, 2015 7:50:42 AM > Subject: [Histonet] B-gal positive control > > Hello Histonet, > > Has anyone out there come across a good FFPE positive control for B-gal? If so, please let me know! We would like to purchase a block or unstained slides if at all possible. > > Thanks! > Anna > > Anna Coffey, MS, HTL(ASCP)CM > Histotechnologist > Center for Advanced Preclinical Research > Frederick National Laboratory for Cancer Research > Leidos Biomedical Research, Inc. > Bld 539, 224 > Frederick, Maryland 21702 > anna.coffey at nih.gov > 301-846-1730 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 12 > Date: Fri, 8 May 2015 16:16:54 +0000 > From: "Weems, Joyce K." > To: "'Campbell, Tasha M.'" , "Vickroy, James" > , "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Billing question > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Special stains are charged per block. If you have blocks A1, A2, and A3, and do AFB and GMS on all three that would be 6 charges. > > Shhhhh... this will probably change next year!! > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems at emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > -----Original Message----- > From: Campbell, Tasha M. [mailto:tmcampbe at fmh.org] > Sent: Friday, May 08, 2015 7:51 AM > To: Vickroy, James; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Billing question > >> From my understanding its just one charge. Its per specimen such as A, B, C, etc. Someone else said that it would be 2 charges so am I wrong on this?? If you did GMS on A1 and AFB on A2 then that would be 2 charges because its two different stains. > > > > > Tasha Campbell, B.S.,HTL(ASCP) > Frederick Gastroenterology Associates > 310 W. 9th St. > Frederick, MD 21701 > 301-695-6800 ext. 144 (w) > 304-685-9307 (c) > > -----Original Message----- > From: Vickroy, James [mailto:jvickroy at SpringfieldClinic.com] > Sent: Thursday, May 07, 2015 4:58 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Billing question > > If I have two sections on an "A" specimen A1 and A2 and both had a GMS stain, do I charge 1 - 88312 or 2 - 88312? > > Jim > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvickroy at SpringfieldClinic.com > > > > This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > > > > ------------------------------ > > Message: 13 > Date: Fri, 8 May 2015 09:41:18 -0700 > From: "Michael Mihalik" > To: > Subject: [Histonet] Friday Trivia Question: Most specimen on a single > case > Message-ID: <012901d089ad$cfd8e4f0$6f8aaed0$@pathview.com> > Content-Type: text/plain; charset="iso-8859-1" > > Please excuse the trivia query, but we've got a client who somewhat > regularly creates cases with 100+ specimen. I think the most I have ever > seen is 127. > > I'm curious how common this is. What's the most specimen on a single case > you've ever seen? > > Thanks for your patience and experience. > > Michael Mihalik > PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 > > > > > > ------------------------------ > > Message: 14 > Date: Fri, 8 May 2015 16:48:31 +0000 > From: Stacy McLaughlin > To: Michael Mihalik , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Friday Trivia Question: Most specimen on a > single case > Message-ID: > > > Content-Type: text/plain; charset="iso-8859-1" > > I've never seen that many on one case. The most I've seen is ~30 (parathyroid) and its been many years. > What types of specimens are they? > Stacy > > -----Original Message----- > From: Michael Mihalik [mailto:mike at pathview.com] > Sent: Friday, May 08, 2015 12:41 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Friday Trivia Question: Most specimen on a single case > > Please excuse the trivia query, but we've got a client who somewhat > regularly creates cases with 100+ specimen. I think the most I have ever > seen is 127. > > I'm curious how common this is. What's the most specimen on a single case > you've ever seen? > > Thanks for your patience and experience. > > Michael Mihalik > PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 15 > Date: Fri, 8 May 2015 16:50:24 +0000 > From: "Marcum, Pamela A" > To: "'Michael Mihalik'" , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Friday Trivia Question: Most specimen on a > single case > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > 157 blocks on one breast case - resident grossed it. > > -----Original Message----- > From: Michael Mihalik [mailto:mike at pathview.com] > Sent: Friday, May 08, 2015 11:41 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Friday Trivia Question: Most specimen on a single case > > Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. > > I'm curious how common this is. What's the most specimen on a single case you've ever seen? > > Thanks for your patience and experience. > > Michael Mihalik > PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > > > ------------------------------ > > Message: 16 > Date: Fri, 8 May 2015 16:51:29 +0000 > From: "Cooper, Brian" > To: "Stacy McLaughlin" , > "Michael Mihalik" , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Friday Trivia Question: Most specimen on a > single case > Message-ID: > > Content-Type: text/plain; charset=iso-8859-1 > > I once saw an entire breast submitted--it was something like 100+ blocks. > > Thanks, > > Brian > > -----Original Message----- > From: Stacy McLaughlin [mailto:Stacy_McLaughlin at cooley-dickinson.org] > Sent: Friday, May 08, 2015 9:49 AM > To: Michael Mihalik; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Friday Trivia Question: Most specimen on a single case > > I've never seen that many on one case. The most I've seen is ~30 (parathyroid) and its been many years. > What types of specimens are they? > Stacy > > -----Original Message----- > From: Michael Mihalik [mailto:mike at pathview.com] > Sent: Friday, May 08, 2015 12:41 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Friday Trivia Question: Most specimen on a single case > > Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. > > I'm curious how common this is. What's the most specimen on a single case you've ever seen? > > Thanks for your patience and experience. > > Michael Mihalik > PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > > > ------------------------------ > > Message: 17 > Date: Fri, 8 May 2015 16:52:42 +0000 > From: "Morken, Timothy" > To: Michael Mihalik , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Friday Trivia Question: Most specimen on a > single case > Message-ID: > <761E2B5697F795489C8710BCC72141FF36830914 at ex07.net.ucsf.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Blocks, yes. Parts of a case (specimens), no. > > -----Original Message----- > From: Michael Mihalik [mailto:mike at pathview.com] > Sent: Friday, May 08, 2015 9:41 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Friday Trivia Question: Most specimen on a single case > > Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. > > I'm curious how common this is. What's the most specimen on a single case you've ever seen? > > Thanks for your patience and experience. > > Michael Mihalik > PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 138, Issue 10 > ***************************************** From ian.bernard at comcast.net Fri May 8 17:05:48 2015 From: ian.bernard at comcast.net (ian bernard) Date: Fri, 8 May 2015 16:05:48 -0600 Subject: [Histonet] Bleach as Cleaning Agent or Decontamination- Message-ID: Our facility is moving towards standardization of decontaminants or disinfectants. They prefer all areas use a Sani wipes that kills most pathogens. However, we contend for Anatomic pathology we need our liquid bleach not only as disinfectant or decontaminant but as a cleaning agent for stained lab-ware. You thoughts? Also, what concentration of Bleach (5.25 or 10%) is acceptable for use as both a disinfectant and cleaning agent or should we keep them separate? We used to buy the hospital grade premade bleach at a 5.25% concentration but now they want us instead to buy the 99.9% commercial Bleach and dilute from there. Any suggestions on opaque containers for us to purchase since bleach break down after a time period, at least for disinfecting? V/r IB From ian.bernard at comcast.net Fri May 8 17:18:46 2015 From: ian.bernard at comcast.net (ian bernard) Date: Fri, 8 May 2015 16:18:46 -0600 Subject: [Histonet] 2015 CAP Inspection Message-ID: <017101d089dc$f3b80c00$db282400$@comcast.net> Another successful AP CAP Inspection with zero discrepancies/zero recommendations! V/r Ian R. Bernard, MSHA, HT (ASCP) HTL (Pend-2015) USAF, MSgt (Retired) Anatomic Pathology Technical Supervisor 10th Medical Group 210-687-7540 Cell ian.bernard at comcast.net ian.bernard.3 at us.af.mil University of Alabama Birmingham- Alumni. From jkiernan at uwo.ca Sat May 9 00:56:05 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Sat, 09 May 2015 00:56:05 -0500 Subject: [Histonet] Bleach as Cleaning Agent or Decontamination- In-Reply-To: <728098371fd5d.554da0d0@uwo.ca> References: <72f0cd211c217.554d8fdf@uwo.ca> <7290e13c1819f.554d901c@uwo.ca> <72d0dc771eed1.554d9096@uwo.ca> <72d092b419817.554d90d4@uwo.ca> <73e095e81ae16.554d9113@uwo.ca> <7370c7e71f537.554d918d@uwo.ca> <72f09bee1f8c7.554d91cb@uwo.ca> <7290cfde1de4f.554d9209@uwo.ca> <72c0ed891e963.554d9248@uwo.ca> <72e0d4901ab24.554d9286@uwo.ca> <72d08e7a1fd90.554d92c5@uwo.ca> <7290c9d019bf1.554d9303@uwo.ca> <7290f3911f347.554d9341@uwo.ca> <72c083e01c5c2.554d937f@uwo.ca> <72a0fd8d18b8f.554d93bd@uwo.ca> <7310ba77181e4.554d9438@uwo.ca> <72e0b0d418e18.554d9476@uwo.ca> <72d0b64a1ed55.554d94b4@uwo.ca> <72a0e8ad1d6b6.554d94f2@uwo.ca> <7280c9ae1ff7b.554d9530@uwo.ca> <72c0bf6618783.554d956e@uwo.ca> <7370a18118fd4.554d95e8@uwo.ca> <7370c2fc1ffb1.554d9626@uwo.ca> <7370f4e61cb95.554d9665@uwo.ca> <72e081e91b664.554d96a3@uwo.ca> <72a0fdb71bedf.554d96e1@uwo.ca> <7290da6d1ca44.554d971f@uwo.ca> <72c0b8a9198aa.554d975e@uwo.ca> <72d0d7841e833.554d97d8@uwo.ca> <7290cc411ec90.554d9816@uwo.ca> <72c0fb0919545.554d9855@uwo.ca> <72c0f5311c6c2.554d9893@uwo.ca> <72d0d54b18c62.554d98d1@uwo.ca> <73e0fd5a19eb8.554d990f@uwo.ca> <72e0e2de189f5.554d994e@uwo.ca> <72e0c9e1181cd.554d998c@uwo.ca> <72d0caa819940.554d9a06@uwo.ca> <7280b0a01c710.554d9a45@uwo.ca> <72a0f7fb1c8bb.554d9a83@uwo.ca> <72a09f291f7c5.554d9ac1@uwo.ca> <73e0aec41b2ba.554d9aff@uwo.ca> <728087821dd23.554d9b3d@uwo.ca> <72e0bce918ad0.554d9b7b@uwo.ca> <72d0d57418a1d.554d9bba@uwo.ca> <72c08ba91d621.554d9bf8@uwo.ca> <728087121fc89.554d9c36@uwo.ca> <72d0e6c11c91f.554d9c74@uwo.ca> <73e0ffd81a9a5.554d9cb2@uwo.ca> <737080f51f9c0.554d9cf0@uwo.ca> <7280885a1e8d8.554d9d2e@uwo.ca> <72d0a79319c75.554d9d6c@uwo.ca> <7290fc9419dac.554d9dab@uwo.ca> <73e081c11e483.554d9de9@uwo.ca> <73e0acdb1f125.554d9e27@uwo.ca> <7310a6611b965.554d9edd@uwo.ca> <72f08dcf18ff6.554d9f1c@uwo.ca> <72e0abf81de90.554d9f5a@uwo.ca> <72c0ed3f1ef6a.554d9f98@uwo.ca> <7300b63d1cbc1.554d9fd7@uwo.ca> <73009c4719e8a.554da015@uwo.ca> <73e0e1201bd96.554da053@uwo.ca> <7370e4dd18ae5.554da092@uwo.ca> <728098371fd5d.554da0d0@uwo.ca> Message-ID: <72a0f90d1f988.554d5b25@uwo.ca> You need to be clear about what the concentration means. Household bleach (such as Javex or Clorox) is a 5% aqueous solution of sodium hypochlorite (NaClO). Sometimes it also contains a polymer (not named on the label) to increase the viscosity. Solutions for adding to swimming pools are 10% aqueous sodium hypochlorite and are cheaper (per gm of NaClO) than household bleach. These pool disinfectants are often labelled "liquid chlorine", a deceptive name that fosters ignorance among those who didn't pay attention at school. Chlorine boils at -34C at atmospheric pressure and exists as a liquid at ambient temperatures only when compressed in railroad tanks or in the (much smaller) gas shells used as weapons by both sides in the First World War. Dakin's solution, used for cleaning dirty wounds, is 0.45-0.50% sodium hypochlorite, made by dilution with a carbonate-bicarbonate buffer to reduce alkalinity. A stronger solution could be used for non-living surfaces. Aqueous solutions of NaClO are "remarkably stable" but the solid compound is extremely unstable. The crystaline pentahydrate melts at 18C and is decomposed by reaction with carbon dioxide from the air;. Anhydrous NaClO is obtainable only by freeze-drying and is "very explosive". Nobody uses the solid. The solutions are made by reaction of chlorine (gas) with aqueous sodium hydroxide solutions. This can also be done by electrolysis of a sodium chloride solution between inert electrodes. My sources of information are bleach bottle labels (small print) and the Merck Index 12th edition, 1996, checked today. Every lab should have a Merck Index on the shelf of reference books! The phrase "99.9% commercial bleach" could not possibly mean 99.9% of either NaOCl or its pentahydrate (solubility 29%). 5.25% (absurd precision!) and 10% bleach probably mean volume dilutions (1:20 or 1:10) of a household hypochlorite bleach without added thickener. A swimming pool 10% sodium hypochlorite solution probably is the cheapest source of chlorine bleach disinfectant without unwanted additives. Dilute it 10-20X with water to swab your possibly infected surfaces. Beware of lab supply companies selling household products at greatly inflated prices. Anyone purporting to sell "hospital grade premade bleach" needs to be viewed with much suspicion. Think before you buy. John Kiernan London, Canada = = = On 08/05/15, ian bernard wrote: > Our facility is moving towards standardization of decontaminants or > disinfectants. They prefer all areas use a Sani wipes that kills most > pathogens. > > ? > > However, we contend for Anatomic pathology we need our liquid bleach not > only as disinfectant or decontaminant but as a cleaning agent for stained > lab-ware. You thoughts? > > ? > > Also, what concentration of Bleach (5.25 or 10%) is acceptable for use as > both a disinfectant and cleaning agent or should we keep them separate? > > ? > > We used to buy the hospital grade premade bleach at a 5.25% concentration > but now they want us instead to buy the 99.9% commercial Bleach and dilute > from there. Any suggestions on opaque containers for us to purchase since > bleach break down after a time period, at least for disinfecting? > > ? > > V/r > > IB > > ? > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Richard.Cartun at hhchealth.org Sat May 9 09:57:47 2015 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Sat, 9 May 2015 14:57:47 +0000 Subject: [Histonet] Friday Trivia Question: Most specimen on a single case In-Reply-To: <012901d089ad$cfd8e4f0$6f8aaed0$@pathview.com> References: <012901d089ad$cfd8e4f0$6f8aaed0$@pathview.com> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E3EE8B5DA@HHCEXCHMB03.hhcsystem.org> The answer to this question will depend on what type of environment you work in. I work at a large tertiary care hospital with a major emphasis on cancer surgery and, as a result, we frequently get major cancer resections (with multiple parts) that generate well over 100 paraffin blocks per case. Also, please note that many current protocols for these types of specimen now dictate for an increased number of paraffin blocks to be submitted. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Michael Mihalik [mailto:mike at pathview.com] Sent: Friday, May 08, 2015 12:41 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Friday Trivia Question: Most specimen on a single case Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. I'm curious how common this is. What's the most specimen on a single case you've ever seen? Thanks for your patience and experience. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From davidkins at yahoo.com Sun May 10 10:34:07 2015 From: davidkins at yahoo.com (David Kinsley) Date: Sun, 10 May 2015 08:34:07 -0700 Subject: [Histonet] Still having Issues with Acetone fixation. In-Reply-To: <3903BE18914F4440834F0E620415FFCA3CB6D082@PPWEXD01d.childrens.sea.kids> Message-ID: <1431272047.18361.YahooMailAndroidMobile@web163103.mail.bf1.yahoo.com> Hi Patrick, I used to use acetone fixation on mouse tissues for cell surface staining. ?I used to cut section at 5 to 10 um and let them air dry for 1 hour. ?I would then fix in 100% acetone for 20 minutes at 4deg C followed by air drying overnight. ?At this point I would either put my sections into wash buffer to begin ihc, or I would wrap them in aluminum foil and store at -80 deg until I was ready to use them. ? I never had an issue with tissue being lost, but I was doing manual ihc. ?Are you using an automated platform for your staining? ?When I used the Dako autostainer with my acetone fixed sections, the blow step would lift the tissue from the slide, so I had to do manual staining. ?The Leica and Ventana platforms are much gentler on the tissues. If you don't have an ihc instrument, I would suggest using the Shandon sequenza staining system for manual ihc. Acetone is a much gentler fixative than formalin or alcohol so you need to be very gentle with the tissue. ?I found that after I completed my ihc staining if I post fixed the slides in formalin for 10 to 20 minutes prior to counterstaining, the morphology and nuclear detail was greatly improved. Let me know if this is helpful. Dave Sent from Yahoo Mail on Android From:"Lewis, Patrick" Date:Thu, May 7, 2015 at 3:13 PM Subject:[Histonet] Still having Issues with Acetone fixation. Hi everyone, Sorry to keep posting about this, But I am still having Acetone issues. I am doing IHC for Cell surface markers that are lost when fixing with etoh or methanol. When I fix in 100% acetone my epitopes have great signal. However,? when I fix in 100% acetone, my tissues all damaged by the acetone beyond all recognition. I can lose up to 90% of my tissue when I fix in 100% acetone for 10 minutes. But, I get good epitope staining if I have some tissue left on the slide. When I fix in anything else, I lose 90% or more of my epitope staining, but my tissue morphology looks great. -- What's the least amount of time I can fix in 100% Acetone for a 5uM section and still have it be fixed? Is drying after the 100% acetone fixing essential? or Bad for protecting tissue morphology? -- I am doing IHC on human tonsils cut to 5 uM with a 24 dry after sectioning. When I fix in 100% acetone, I fix it at 4C for 10 minutes. Then dry for 1 hour in the fume hood Should it go straight into buffer? Should it be for less time in the acetone? Should the acetone be Room temp or -20C instead of 4C. If I was diluting the acetone with buffer, (or etoh) then I could see going straight into buffer afterwards, but because I am using 100% I think that going into liquid right after fixing is too much of a change and my tissues go BOOM. Please help. Patrick Lewis Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks at gmail.com Sun May 10 10:52:45 2015 From: amosbrooks at gmail.com (Amos Brooks) Date: Sun, 10 May 2015 11:52:45 -0400 Subject: [Histonet] B-gal positive control Message-ID: Hi, Normal kidney should work fine for this. Amos On Fri, May 8, 2015 at 1:00 PM, wrote: > Message: 9 > Date: Fri, 8 May 2015 14:50:42 +0000 > From: "Coffey, Anna (NIH/NCI) [C]" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] B-gal positive control > Message-ID: <5C3E10119A1B824FBE92B08279F74A91017995AF at msgb10.nih.gov> > Content-Type: text/plain; charset="us-ascii" > > Hello Histonet, > > Has anyone out there come across a good FFPE positive control for B-gal? > If so, please let me know! We would like to purchase a block or unstained > slides if at all possible. > > Thanks! > Anna > > Anna Coffey, MS, HTL(ASCP)CM > Histotechnologist > From j.benavides at eae.csic.es Sun May 10 10:56:51 2015 From: j.benavides at eae.csic.es (Julio Benavides) Date: Sun, 10 May 2015 17:56:51 +0200 Subject: [Histonet] Still having Issues with Acetone fixation. In-Reply-To: <1431272047.18361.YahooMailAndroidMobile@web163103.mail.bf1.yahoo.com> References: <1431272047.18361.YahooMailAndroidMobile@web163103.mail.bf1.yahoo.com> Message-ID: <20150510175651.Horde.z58uzCgoT8t3BwO5TUdUZg1@webmail.csic.es> HI David, how did you do the post IHC fixation? immersion in buffered formalin 4%? Looks interesting Thanks a lot Julio David Kinsley escribi?: > Hi Patrick, > > I used to use acetone fixation on mouse tissues for cell surface > staining. ?I used to cut section at 5 to 10 um and let them air dry > for 1 hour. ?I would then fix in 100% acetone for 20 minutes at 4deg > C followed by air drying overnight. ?At this point I would either > put my sections into wash buffer to begin ihc, or I would wrap them > in aluminum foil and store at -80 deg until I was ready to use them. ? > > > I never had an issue with tissue being lost, but I was doing manual > ihc. ?Are you using an automated platform for your staining? ?When I > used the Dako autostainer with my acetone fixed sections, the blow > step would lift the tissue from the slide, so I had to do manual > staining. ?The Leica and Ventana platforms are much gentler on the > tissues. > > > If you don't have an ihc instrument, I would suggest using the > Shandon sequenza staining system for manual ihc. > > > Acetone is a much gentler fixative than formalin or alcohol so you > need to be very gentle with the tissue. ?I found that after I > completed my ihc staining if I post fixed the slides in formalin for > 10 to 20 minutes prior to counterstaining, the morphology and > nuclear detail was greatly improved. > > > Let me know if this is helpful. > > > Dave > > Sent from Yahoo Mail on Android > > From:"Lewis, Patrick" > Date:Thu, May 7, 2015 at 3:13 PM > Subject:[Histonet] Still having Issues with Acetone fixation. > > Hi everyone, > > Sorry to keep posting about this, > > But I am still having Acetone issues. > > I am doing IHC for Cell surface markers that are lost when fixing > with etoh or methanol. > > When I fix in 100% acetone my epitopes have great signal. > > However,? when I fix in 100% acetone, my tissues all damaged by the > acetone beyond all recognition. > > I can lose up to 90% of my tissue when I fix in 100% acetone for 10 minutes. > > But, I get good epitope staining if I have some tissue left on the slide. > > When I fix in anything else, I lose 90% or more of my epitope > staining, but my tissue morphology looks great. > > -- > What's the least amount of time I can fix in 100% Acetone for a 5uM > section and still have it be fixed? > > Is drying after the 100% acetone fixing essential? or Bad for > protecting tissue morphology? > > -- > I am doing IHC on human tonsils cut to 5 uM with a 24 dry after sectioning. > > When I fix in 100% acetone, I fix it at 4C for 10 minutes. Then dry > for 1 hour in the fume hood > > Should it go straight into buffer? Should it be for less time in the acetone? > Should the acetone be Room temp or -20C instead of 4C. > > If I was diluting the acetone with buffer, (or etoh) then I could > see going straight into buffer afterwards, but because I am using > 100% I think that going into liquid right after fixing is too much > of a change and my tissues go BOOM. > > Please help. > > Patrick Lewis > > > Patrick Lewis > Research Associate II Bench > Seattle Childrens Research Institute > 206-884-1115 > > CONFIDENTIALITY NOTICE: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and > may contain confidential and privileged information protected by > law. Any unauthorized review, use, disclosure or distribution is > prohibited. If you are not the intended recipient, please contact > the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr at comcast.net Sun May 10 12:08:56 2015 From: koellingr at comcast.net (koellingr at comcast.net) Date: Sun, 10 May 2015 17:08:56 +0000 (UTC) Subject: [Histonet] B-gal positive control In-Reply-To: References: Message-ID: <904386300.6969863.1431277736299.JavaMail.zimbra@comcast.net> Amos, hello.? Do you have a reference for this?? All my files talk about "endogenous" B-gal in kidney and pancreas and other organs (but then article talks about after lacZ transgenic manipulation) or demonstration of alpha-galactosidase in kidneys or in senescence associated or lysozomal storage diseases or differentiating?light background staining?from lacZ with pH tricks or pictures of islet cells staining with surrounding exocrine pancreas staining or just the journalistic form of hand-waving "data not shown".? Is beta-galactosidase readily expressed in completely normal kidney and where specifically? Thanks, Ray ----- Original Message ----- From: "Amos Brooks" To: histonet at lists.utsouthwestern.edu Sent: Sunday, May 10, 2015 8:52:45 AM Subject: [Histonet] ?B-gal positive control Hi, ?? ? Normal kidney should work fine for this. Amos On Fri, May 8, 2015 at 1:00 PM, wrote: > Message: 9 > Date: Fri, 8 May 2015 14:50:42 +0000 > From: "Coffey, Anna (NIH/NCI) [C]" > To: "histonet at lists.utsouthwestern.edu" > ? ? ? ? > Subject: [Histonet] B-gal positive control > Message-ID: <5C3E10119A1B824FBE92B08279F74A91017995AF at msgb10.nih.gov> > Content-Type: text/plain; charset="us-ascii" > > Hello Histonet, > > Has anyone out there come across a good FFPE positive control for B-gal? > If so, please let me know! We would like to purchase a block or unstained > slides if at all possible. > > Thanks! > Anna > > Anna Coffey, MS, HTL(ASCP)CM > Histotechnologist > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ssegal2 at slu.edu Sun May 10 12:11:25 2015 From: ssegal2 at slu.edu (Salomao Segal) Date: Sun, 10 May 2015 12:11:25 -0500 Subject: [Histonet] optic chiasmata Message-ID: I intend to use a cryostat to cut 70 - 100 micron thick sections of human optic chiasmata. Tissue is cryoprotected with 30% sucrose solution. My question relates to the freezing process per se. Would it be enough to place the tissue in a -4 freezer to harden and then transfer to the cryostat chamber at say -20 wait a while and cut? Or is it necessary to introduce an intermediary step for freezing? Thanks SS *Solomon Segal, M.D.* *Associate Professor of Anatomy in SurgeryCenter for Anatomical Science and Education (CASE)Department of SurgerySchool of MedicineSaint Louis University* *1402 South Grand Blvd.* *Schwitalla Hall - 3rd Floor - M310* *Saint Louis, MO, 63104office: 314 977 8023laboratory: 314 977 8080* *CASE: 314 977 8027FAX: 314 977 5127e-mail*: ssegal2 at slu.edu http://medschool.slu.edu/anatomy/ http://slu.academia.edu/SolomonSegal https://sites.google.com/a/slu.edu/segal-laboratory/ https://sites.google.com/a/slu.edu/dr-segal-s-clinical-anatomy-website/ From jkiernan at uwo.ca Sun May 10 21:14:15 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Sun, 10 May 2015 21:14:15 -0500 Subject: [Histonet] optic chiasmata In-Reply-To: <73e08fc21e883.55501066@uwo.ca> References: <73e08fc21e883.55501066@uwo.ca> Message-ID: <72d0d4091da06.554fca27@uwo.ca> Slow freezing results in formation of ice crystals. In brain tissue (fresh or formaldehyde-fixed) these destroy the architecture, which is replaced by a sponge-like texture of approximately cell-sized holes. Cryoprotection of fixed tissue with 30% sucrose ameliorates this, but freezing at -4C or -20C is asking too much of the cryoprotective action. You need a surface at or below -80C. A cryostat chuck standing in a slush of solid CO2 and acetone is OK. Isopentane in a small metal can standing in liquid nitrogen is better. Test your technique first on an unimportant piece of white matter about the same thickness as the optic chiasma, and cut test sections through different levels in the specimen, because some parts will freeze more slowly than others. John A. Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada = = = On 10/05/15, Salomao Segal wrote: > I intend to use a cryostat to cut 70 - 100 micron thick sections of human > optic chiasmata. > > Tissue is cryoprotected with 30% sucrose solution. > > My question relates to the freezing process per se. > > Would it be enough to place the tissue in a -4 freezer to harden and then > transfer to the cryostat chamber at say -20 wait a while and cut? Or is it > necessary to introduce an intermediary step for freezing? > > Thanks > > SS > > > *Solomon Segal, M.D.* > > > > > *Associate Professor of Anatomy in SurgeryCenter for Anatomical Science and > Education (CASE)Department of SurgerySchool of MedicineSaint Louis > University* > > *1402 South Grand Blvd.* > *Schwitalla Hall - 3rd Floor - M310* > > > *Saint Louis, MO, 63104office: 314 977 8023laboratory: 314 977 8080* > > > *CASE: 314 977 8027FAX: 314 977 5127e-mail*: ssegal2 at slu.edu > > http://medschool.slu.edu/anatomy/ > > http://slu.academia.edu/SolomonSegal > > https://sites.google.com/a/slu.edu/segal-laboratory/ > > https://sites.google.com/a/slu.edu/dr-segal-s-clinical-anatomy-website/ > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From annigyg at gmail.com Sun May 10 21:43:58 2015 From: annigyg at gmail.com (Anne Van Binsbergen) Date: Mon, 11 May 2015 06:43:58 +0400 Subject: [Histonet] IHC breast markers: changing source of NBF Message-ID: <025FB04F-A5DC-4532-BACD-D970057AC942@gmail.com> Greetings esteemed Histonetters We use commercially prepared NBF in pre-filled containers for collection and submission of all tissue. We use 'home-made' NBF ( Millonigs as per Freda Carson) in the holding tanks on the gross station and in all our tissue processors. We also strictly adhere to CAP regulations re fixation times for all breast tissue (6-72 hrs) - - our preferred lower end cut-off is 8 hrs. Recently we have had some variable IHC breast marker staining outcomes. Question: Could the use of 2 'different ' sources of NBF affect the staining of predictive markers? Your expert opinion is highly appreciated Regards from SandyLands annieinarabia Sent from my iPhone From mlens at ITS.JNJ.com Mon May 11 03:32:53 2015 From: mlens at ITS.JNJ.com (Lens, Maximiliaan [RNDBE Non-J&J]) Date: Mon, 11 May 2015 08:32:53 +0000 Subject: [Histonet] Methylmethacrylate embedded bone Message-ID: <8CB7E46B4A4D984783DAE714966E69CAB15AEE@ITSBEBEGMDGS04.jnj.com> Dear, I have a lot of problems of stretching my 6 micrometer slices of methylmethacrylate bone for histomorphometry. I have already used different ethanol concentrations and even ethanol baths, but nothing seems to work. Does anyone have any experience with this and knows what to do? I also have some issues with staining procedures: I have 2 slices, 1 embedded by me with Technovit 9100 and 1 embedded by a CRO. If I perform a staining procedure the second stains perfectly, but my bones stain more diffuse and aspecific. Although they are stained in the same run and thus same protocol. Thanks in advance. Maximiliaan Lens From simmca at UPMC.EDU Mon May 11 09:31:47 2015 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Mon, 11 May 2015 14:31:47 +0000 Subject: [Histonet] Job Openings at UPMC Dermatology Message-ID: <6295CCF61B0DBB4C9248F51F8940E6A10118DA7F@MSXMBXNSPRD09.acct.upmchs.net> Hello Histonet! The Department of Dermatology at UPMC has 2 Histotechnician positions available for immediate fill in their Dermatopathology Histology Laboratory. The laboratory is in the Medical Arts Building in Oakland in a new space. Windows and a 5th floor view of Oakland! The position requires you have your HT or your HT within 1-year. You must be able to gross only skin samples, we can train you how to! M-F daylight flexible schedule! No Holidays, No Weekends! This is the histology position you have been looking for! Go to UPMC.com and search for job code 2074762 and/or 2074067 You can contact me at the numbers below to discuss. All the best! Chris Simmons B.S., A.S., HTL(ASCP) Supervisor, UPP Dermatopathology 412.864.3880 office 412.612.0881 cell From amanda.krempley at abbvie.com Mon May 11 14:52:18 2015 From: amanda.krempley at abbvie.com (Krempley, Amanda L) Date: Mon, 11 May 2015 19:52:18 +0000 Subject: [Histonet] anti-human IgG antibody Message-ID: <9667996145fe4392bc5c71b3f3a8ae94@USAASECSM048.R0018.COLLABORATION.ECS.HP.COM> Hello Histoland, Has anyone used an anti-human IgG antibody that does not cross react with Non-human primates, specifically cynomolgus, for IHC? Could you please share the antibody and protocol that you have had success with? Thank you, Amanda From WaitT at livemail.uthscsa.edu Mon May 11 15:26:14 2015 From: WaitT at livemail.uthscsa.edu (Wait, Trevor Jordan) Date: Mon, 11 May 2015 20:26:14 +0000 Subject: [Histonet] TRAP Stain Help Message-ID: <1431375976414.44600@livemail.uthscsa.edu> Hey guys, recently I've used the Sigma Aldrich TRAP Stain Kit in order to stain for Osteoclasts in Formalin Fixed Paraffin Embedded bone tissues that are EDTA decalcified. Unfortunately there was no TRAP stain to be found whatsoever on all slides that I stained. I was hoping that someone with some experience with TRAP stain could really help me out! Here are a couple of reasons that I have wondered as to why the TRAP stain might not be visible.... 1. Staining too long with Hematoxylin counterstain? I have noticed in several trial runs that sometimes if the Hematoxylin counterstain is too long then this can effect the amount of TRAP stain that shows up with the osteoclasts. Perhaps I'm just over analyzing.... 2. Perhaps no osteoclasts were present to even stain....does anyone know what the usual ratio of Osteoclasts to Osteocytes are? In the past stains that I have used....whenever there is an osteoclasts that shows up...it is usually pretty spotty and many times they are in groups together...is this how it normally is? 3. Perhaps the TRAP stain is being washed away through the rinsing with dI or dehydration with ethanol/clearing just before mounting. A protocol that I used before the Sigma Aldrich Kit incorporates incubating the slides in 37 celsius water for 1 hour just prior to allowing the slides to sit in the TRAP stain solution (this is also 37 celsius in the same water bath) and letting it sit for 20 minutes. With this stain...there seems to be a consistent showing of osteoclasts but I'm just not sure if all of the osteoclasts are showing up correctly...that is why I moved to the Sigma Aldrich kit to make sure and the results didn't show ANY osteoclasts there! Anyways...I'm just curious if there are any reasons that the TRAP stain is not showing up...I would appreciate any input! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate From patpxs at gmail.com Mon May 11 15:57:21 2015 From: patpxs at gmail.com (Paula Sicurello) Date: Mon, 11 May 2015 13:57:21 -0700 Subject: [Histonet] Per Diem Positions at UC San Diego Health System Message-ID: Good Afternoon Listers, UCSD Medical Center Department of Anatomic Pathology has two per diem positions open in Histology. Histology experience is a must. If you are interested email your resumes to: Craig Fisher at *cfisher at ucsd.edu * and Mike Mathhew at *mimatthew at ucsd.edu * Thank you and have a nice day. Paula :-) From patpxs at gmail.com Mon May 11 16:03:40 2015 From: patpxs at gmail.com (Paula Sicurello) Date: Mon, 11 May 2015 14:03:40 -0700 Subject: [Histonet] H&E Stainer Question Message-ID: Me again... UCSD is in the market for a new H&E stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) From Royl1 at LabCorp.com Tue May 12 07:18:46 2015 From: Royl1 at LabCorp.com (Roy, Lisa) Date: Tue, 12 May 2015 12:18:46 +0000 Subject: [Histonet] H&E Stainer Question In-Reply-To: References: Message-ID: Paula Here are my two cents.... I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.....no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. Hope it helps....good luck. Lisa -----Original Message----- From: Paula Sicurello [mailto:patpxs at gmail.com] Sent: Monday, May 11, 2015 5:04 PM To: HistoNet Subject: [Histonet] H&E Stainer Question Me again... UCSD is in the market for a new H&E stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. From jqb7 at cdc.gov Tue May 12 07:26:38 2015 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue, 12 May 2015 12:26:38 +0000 Subject: [Histonet] H&E Stainer Question In-Reply-To: References: Message-ID: <3B2CD438E1628A41BD687E98B963B78137F3A0D3@EMBX-CLFT4.cdc.gov> This is what we have Leica) and it has 4 ovens. http://www.leica-microsystems.com/news-media/news/news-details/article/leica-st5020-multistainer-workstation/ We also have a Prisma and we like them both. We have had the Leica longer and it just never breaks down.....knock on wood! We have to have glass coverslips and the Prisma glass coverslipper is a bit more finicky than the Leica. -----Original Message----- From: Roy, Lisa [mailto:Royl1 at LabCorp.com] Sent: Tuesday, May 12, 2015 8:19 AM To: Paula Sicurello; HistoNet Subject: Re: [Histonet] H&E Stainer Question Paula Here are my two cents.... I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.....no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. Hope it helps....good luck. Lisa -----Original Message----- From: Paula Sicurello [mailto:patpxs at gmail.com] Sent: Monday, May 11, 2015 5:04 PM To: HistoNet Subject: [Histonet] H&E Stainer Question Me again... UCSD is in the market for a new H&E stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From simmca at UPMC.EDU Tue May 12 07:32:43 2015 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Tue, 12 May 2015 12:32:43 +0000 Subject: [Histonet] H&E Stainer Question In-Reply-To: References: Message-ID: <6295CCF61B0DBB4C9248F51F8940E6A10118F112@MSXMBXNSPRD09.acct.upmchs.net> To be fair, a "batch" of slides for Leica is actually 270- slides, It can run 9 racks at a time, but, the 9th rack from start to coverslip is 3 hours+ You can always skip the on-board oven and place your slides in a slide dryer (most labs have them) and then every 3 minutes you can load a rack (1st xylene step 3 minutes) Then it goes much faster. As for tape..ugh..it is only guaranteed to last 7 years, after that they pull off the slide and take the tissue with it. CAP is starting to frown on this as you need to keep the initial H&E slides for up to 10+ years. Chris Simmons B.S., A.S., HTL(ASCP) Supervisor, UPP Dermatopathology 412.864.3880 office 412.612.0881 cell -----Original Message----- From: Roy, Lisa [mailto:Royl1 at LabCorp.com] Sent: Tuesday, May 12, 2015 8:19 AM To: Paula Sicurello; HistoNet Subject: Re: [Histonet] H&E Stainer Question Paula Here are my two cents.... I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.....no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. Hope it helps....good luck. Lisa -----Original Message----- From: Paula Sicurello [mailto:patpxs at gmail.com] Sent: Monday, May 11, 2015 5:04 PM To: HistoNet Subject: [Histonet] H&E Stainer Question Me again... UCSD is in the market for a new H&E stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Royl1 at LabCorp.com Tue May 12 07:36:49 2015 From: Royl1 at LabCorp.com (Roy, Lisa) Date: Tue, 12 May 2015 12:36:49 +0000 Subject: [Histonet] H&E Stainer Question In-Reply-To: <6295CCF61B0DBB4C9248F51F8940E6A10118F112@MSXMBXNSPRD09.acct.upmchs.net> References: <6295CCF61B0DBB4C9248F51F8940E6A10118F112@MSXMBXNSPRD09.acct.upmchs.net> Message-ID: Depends on which model you have...hence XL. Ours only has one oven so you're really only getting 3-4 racks stained at a time. -----Original Message----- From: Simmons, Christopher [mailto:simmca at UPMC.EDU] Sent: Tuesday, May 12, 2015 8:33 AM To: Roy, Lisa; Paula Sicurello; HistoNet Subject: RE: [Histonet] H&E Stainer Question To be fair, a "batch" of slides for Leica is actually 270- slides, It can run 9 racks at a time, but, the 9th rack from start to coverslip is 3 hours+ You can always skip the on-board oven and place your slides in a slide dryer (most labs have them) and then every 3 minutes you can load a rack (1st xylene step 3 minutes) Then it goes much faster. As for tape..ugh..it is only guaranteed to last 7 years, after that they pull off the slide and take the tissue with it. CAP is starting to frown on this as you need to keep the initial H&E slides for up to 10+ years. Chris Simmons B.S., A.S., HTL(ASCP) Supervisor, UPP Dermatopathology 412.864.3880 office 412.612.0881 cell -----Original Message----- From: Roy, Lisa [mailto:Royl1 at LabCorp.com] Sent: Tuesday, May 12, 2015 8:19 AM To: Paula Sicurello; HistoNet Subject: Re: [Histonet] H&E Stainer Question Paula Here are my two cents.... I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.....no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. Hope it helps....good luck. Lisa -----Original Message----- From: Paula Sicurello [mailto:patpxs at gmail.com] Sent: Monday, May 11, 2015 5:04 PM To: HistoNet Subject: [Histonet] H&E Stainer Question Me again... UCSD is in the market for a new H&E stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. From relia1 at earthlink.net Tue May 12 09:21:37 2015 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 12 May 2015 10:21:37 -0400 Subject: [Histonet] RELIA HOT JOB Alert! Lead Histotech needed for Brand New Lab in Dallas/Ft. Worth. A RELIA EXCLUSIVE!!! Message-ID: <00e201d08cbe$f5053b20$df0fb160$@earthlink.net> Hi Histonetters!! How are you? I have an exciting opportunity that just might interest you. OR If you happen to know someone qualified who might be interested I welcome you to refer them. If I place them you will earn a referral fee. The position we have been engaged to work on is an ASCP Certified histotech with dermpath experience (Mohs is a plus and my client will train). This is for a BRAND NEW LAB located in the Dallas/Fort Worth area. This person will be the sole practitioner histotech in this brand new lab. You will be responsible for managing and maintaining the lab and performing histology. My client offers a competitive salary, nice benefits and an outstanding opportunity. For more information please contact me Pam Barker at relia1 at earthlink.net or toll free at 866-607-3542. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From joelleweaver at hotmail.com Tue May 12 11:26:58 2015 From: joelleweaver at hotmail.com (Joelle Weaver) Date: Tue, 12 May 2015 16:26:58 +0000 Subject: [Histonet] H&E Stainer Question In-Reply-To: References: , Message-ID: Personally I love the Prisma for volume and the tape. I know many have bad opinions, but I wish I had both the Prisma and the tape right now! I have never seen any problems with very old ( 15+ year) slides. using the tape. Not saying it can't happen-but have not personally seen it. The tape is easier to get off if you need to versus old glass CS, just use acetone, acetone/xylene, xylene. Comes off in a gel form and slides right off leaving the tissue intact. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Royl1 at LabCorp.com > To: patpxs at gmail.com; histonet at lists.utsouthwestern.edu > Date: Tue, 12 May 2015 12:18:46 +0000 > Subject: Re: [Histonet] H&E Stainer Question > > Paula > Here are my two cents.... > > I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. > > On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.....no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. > > Hope it helps....good luck. > Lisa > > -----Original Message----- > From: Paula Sicurello [mailto:patpxs at gmail.com] > Sent: Monday, May 11, 2015 5:04 PM > To: HistoNet > Subject: [Histonet] H&E Stainer Question > > Me again... > > UCSD is in the market for a new H&E stainer for our new hospital opening next year. > > We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. > > What do you use? > > Suggestions gratefully accepted-even from you two Keith and Matt ;) > > Opinions about the good, the bad, and the ugly (as long as it works really > well) will be helpful. > > Thanks oodles! > > Paula :-) > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sarah_Mack at urmc.rochester.edu Tue May 12 12:04:20 2015 From: Sarah_Mack at urmc.rochester.edu (Mack, Sarah) Date: Tue, 12 May 2015 13:04:20 -0400 Subject: [Histonet] TRAP Stain Help In-Reply-To: References: Message-ID: Travis, Were your samples stored in alcohol? If so, that can kill the TRAP enzyme. Your protocol is similar to ours. Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research Histology, Biochemistry, and Molecular Imaging Core 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-3901 ________________________________________ From: histonet-request at lists.utsouthwestern.edu [histonet-request at lists.utsouthwestern.edu] Sent: Tuesday, May 12, 2015 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 138, Issue 14 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=ojqKLAQtVhO8ixOrdIb13M0riQkWk2GB5BRQSgbZ3_Q&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. anti-human IgG antibody (Krempley, Amanda L) 2. TRAP Stain Help (Wait, Trevor Jordan) 3. Per Diem Positions at UC San Diego Health System (Paula Sicurello) 4. H&E Stainer Question (Paula Sicurello) 5. Re: H&E Stainer Question (Roy, Lisa) 6. Re: H&E Stainer Question (Sanders, Jeanine (CDC/OID/NCEZID)) 7. Re: H&E Stainer Question (Simmons, Christopher) 8. Re: H&E Stainer Question (Roy, Lisa) 9. RELIA HOT JOB Alert! Lead Histotech needed for Brand New Lab in Dallas/Ft. Worth. A RELIA EXCLUSIVE!!! (Pam Barker) 10. Re: H&E Stainer Question (Joelle Weaver) ---------------------------------------------------------------------- Message: 1 Date: Mon, 11 May 2015 19:52:18 +0000 From: "Krempley, Amanda L" To: HistoNet Subject: [Histonet] anti-human IgG antibody Message-ID: <9667996145fe4392bc5c71b3f3a8ae94 at USAASECSM048.R0018.COLLABORATION.ECS.HP.COM> Content-Type: text/plain; charset="utf-8" Hello Histoland, Has anyone used an anti-human IgG antibody that does not cross react with Non-human primates, specifically cynomolgus, for IHC? Could you please share the antibody and protocol that you have had success with? Thank you, Amanda ------------------------------ Message: 2 Date: Mon, 11 May 2015 20:26:14 +0000 From: "Wait, Trevor Jordan" To: "Histonet at Lists. Edu" Subject: [Histonet] TRAP Stain Help Message-ID: <1431375976414.44600 at livemail.uthscsa.edu> Content-Type: text/plain; charset="iso-8859-1" Hey guys, recently I've used the Sigma Aldrich TRAP Stain Kit in order to stain for Osteoclasts in Formalin Fixed Paraffin Embedded bone tissues that are EDTA decalcified. Unfortunately there was no TRAP stain to be found whatsoever on all slides that I stained. I was hoping that someone with some experience with TRAP stain could really help me out! Here are a couple of reasons that I have wondered as to why the TRAP stain might not be visible.... 1. Staining too long with Hematoxylin counterstain? I have noticed in several trial runs that sometimes if the Hematoxylin counterstain is too long then this can effect the amount of TRAP stain that shows up with the osteoclasts. Perhaps I'm just over analyzing.... 2. Perhaps no osteoclasts were present to even stain....does anyone know what the usual ratio of Osteoclasts to Osteocytes are? In the past stains that I have used....whenever there is an osteoclasts that shows up...it is usually pretty spotty and many times they are in groups together...is this how it normally is? 3. Perhaps the TRAP stain is being washed away through the rinsing with dI or dehydration with ethanol/clearing just before mounting. A protocol that I used before the Sigma Aldrich Kit incorporates incubating the slides in 37 celsius water for 1 hour just prior to allowing the slides to sit in the TRAP stain solution (this is also 37 celsius in the same water bath) and letting it sit for 20 minutes. With this stain...there seems to be a consistent showing of osteoclasts but I'm just not sure if all of the osteoclasts are showing up correctly...that is why I moved to the Sigma Aldrich kit to make sure and the results didn't show ANY osteoclasts there! Anyways...I'm just curious if there are any reasons that the TRAP stain is not showing up...I would appreciate any input! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate ------------------------------ Message: 3 Date: Mon, 11 May 2015 13:57:21 -0700 From: Paula Sicurello To: HistoNet Subject: [Histonet] Per Diem Positions at UC San Diego Health System Message-ID: Content-Type: text/plain; charset=UTF-8 Good Afternoon Listers, UCSD Medical Center Department of Anatomic Pathology has two per diem positions open in Histology. Histology experience is a must. If you are interested email your resumes to: Craig Fisher at *cfisher at ucsd.edu * and Mike Mathhew at *mimatthew at ucsd.edu * Thank you and have a nice day. Paula :-) ------------------------------ Message: 4 Date: Mon, 11 May 2015 14:03:40 -0700 From: Paula Sicurello To: HistoNet Subject: [Histonet] H&E Stainer Question Message-ID: Content-Type: text/plain; charset=UTF-8 Me again... UCSD is in the market for a new H&E stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) ------------------------------ Message: 5 Date: Tue, 12 May 2015 12:18:46 +0000 From: "Roy, Lisa" To: Paula Sicurello , HistoNet Subject: Re: [Histonet] H&E Stainer Question Message-ID: Content-Type: text/plain; charset="us-ascii" Paula Here are my two cents.... I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.....no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. Hope it helps....good luck. Lisa -----Original Message----- From: Paula Sicurello [mailto:patpxs at gmail.com] Sent: Monday, May 11, 2015 5:04 PM To: HistoNet Subject: [Histonet] H&E Stainer Question Me again... UCSD is in the market for a new H&E stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=ojqKLAQtVhO8ixOrdIb13M0riQkWk2GB5BRQSgbZ3_Q&e= -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ------------------------------ Message: 6 Date: Tue, 12 May 2015 12:26:38 +0000 From: "Sanders, Jeanine (CDC/OID/NCEZID)" To: "Roy, Lisa" , Paula Sicurello , HistoNet Subject: Re: [Histonet] H&E Stainer Question Message-ID: <3B2CD438E1628A41BD687E98B963B78137F3A0D3 at EMBX-CLFT4.cdc.gov> Content-Type: text/plain; charset="us-ascii" This is what we have Leica) and it has 4 ovens. https://urldefense.proofpoint.com/v2/url?u=http-3A__www.leica-2Dmicrosystems.com_news-2Dmedia_news_news-2Ddetails_article_leica-2Dst5020-2Dmultistainer-2Dworkstation_&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=1Jk81pXE6-81arrP_81cZthjnCrrpONTB8phTSHW8hE&e= We also have a Prisma and we like them both. We have had the Leica longer and it just never breaks down.....knock on wood! We have to have glass coverslips and the Prisma glass coverslipper is a bit more finicky than the Leica. -----Original Message----- From: Roy, Lisa [mailto:Royl1 at LabCorp.com] Sent: Tuesday, May 12, 2015 8:19 AM To: Paula Sicurello; HistoNet Subject: Re: [Histonet] H&E Stainer Question Paula Here are my two cents.... I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.....no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. Hope it helps....good luck. Lisa -----Original Message----- From: Paula Sicurello [mailto:patpxs at gmail.com] Sent: Monday, May 11, 2015 5:04 PM To: HistoNet Subject: [Histonet] H&E Stainer Question Me again... UCSD is in the market for a new H&E stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=ojqKLAQtVhO8ixOrdIb13M0riQkWk2GB5BRQSgbZ3_Q&e= -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=ojqKLAQtVhO8ixOrdIb13M0riQkWk2GB5BRQSgbZ3_Q&e= ------------------------------ Message: 7 Date: Tue, 12 May 2015 12:32:43 +0000 From: "Simmons, Christopher" To: "'Roy, Lisa'" , Paula Sicurello , HistoNet Subject: Re: [Histonet] H&E Stainer Question Message-ID: <6295CCF61B0DBB4C9248F51F8940E6A10118F112 at MSXMBXNSPRD09.acct.upmchs.net> Content-Type: text/plain; charset="us-ascii" To be fair, a "batch" of slides for Leica is actually 270- slides, It can run 9 racks at a time, but, the 9th rack from start to coverslip is 3 hours+ You can always skip the on-board oven and place your slides in a slide dryer (most labs have them) and then every 3 minutes you can load a rack (1st xylene step 3 minutes) Then it goes much faster. As for tape..ugh..it is only guaranteed to last 7 years, after that they pull off the slide and take the tissue with it. CAP is starting to frown on this as you need to keep the initial H&E slides for up to 10+ years. Chris Simmons B.S., A.S., HTL(ASCP) Supervisor, UPP Dermatopathology 412.864.3880 office 412.612.0881 cell -----Original Message----- From: Roy, Lisa [mailto:Royl1 at LabCorp.com] Sent: Tuesday, May 12, 2015 8:19 AM To: Paula Sicurello; HistoNet Subject: Re: [Histonet] H&E Stainer Question Paula Here are my two cents.... I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.....no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. Hope it helps....good luck. Lisa -----Original Message----- From: Paula Sicurello [mailto:patpxs at gmail.com] Sent: Monday, May 11, 2015 5:04 PM To: HistoNet Subject: [Histonet] H&E Stainer Question Me again... UCSD is in the market for a new H&E stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=ojqKLAQtVhO8ixOrdIb13M0riQkWk2GB5BRQSgbZ3_Q&e= -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=ojqKLAQtVhO8ixOrdIb13M0riQkWk2GB5BRQSgbZ3_Q&e= ------------------------------ Message: 8 Date: Tue, 12 May 2015 12:36:49 +0000 From: "Roy, Lisa" To: "Simmons, Christopher" , Paula Sicurello , HistoNet Subject: Re: [Histonet] H&E Stainer Question Message-ID: Content-Type: text/plain; charset="us-ascii" Depends on which model you have...hence XL. Ours only has one oven so you're really only getting 3-4 racks stained at a time. -----Original Message----- From: Simmons, Christopher [mailto:simmca at UPMC.EDU] Sent: Tuesday, May 12, 2015 8:33 AM To: Roy, Lisa; Paula Sicurello; HistoNet Subject: RE: [Histonet] H&E Stainer Question To be fair, a "batch" of slides for Leica is actually 270- slides, It can run 9 racks at a time, but, the 9th rack from start to coverslip is 3 hours+ You can always skip the on-board oven and place your slides in a slide dryer (most labs have them) and then every 3 minutes you can load a rack (1st xylene step 3 minutes) Then it goes much faster. As for tape..ugh..it is only guaranteed to last 7 years, after that they pull off the slide and take the tissue with it. CAP is starting to frown on this as you need to keep the initial H&E slides for up to 10+ years. Chris Simmons B.S., A.S., HTL(ASCP) Supervisor, UPP Dermatopathology 412.864.3880 office 412.612.0881 cell -----Original Message----- From: Roy, Lisa [mailto:Royl1 at LabCorp.com] Sent: Tuesday, May 12, 2015 8:19 AM To: Paula Sicurello; HistoNet Subject: Re: [Histonet] H&E Stainer Question Paula Here are my two cents.... I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.....no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. Hope it helps....good luck. Lisa -----Original Message----- From: Paula Sicurello [mailto:patpxs at gmail.com] Sent: Monday, May 11, 2015 5:04 PM To: HistoNet Subject: [Histonet] H&E Stainer Question Me again... UCSD is in the market for a new H&E stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=ojqKLAQtVhO8ixOrdIb13M0riQkWk2GB5BRQSgbZ3_Q&e= -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=ojqKLAQtVhO8ixOrdIb13M0riQkWk2GB5BRQSgbZ3_Q&e= -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ------------------------------ Message: 9 Date: Tue, 12 May 2015 10:21:37 -0400 From: "Pam Barker" To: "Histonet" Subject: [Histonet] RELIA HOT JOB Alert! Lead Histotech needed for Brand New Lab in Dallas/Ft. Worth. A RELIA EXCLUSIVE!!! Message-ID: <00e201d08cbe$f5053b20$df0fb160$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters!! How are you? I have an exciting opportunity that just might interest you. OR If you happen to know someone qualified who might be interested I welcome you to refer them. If I place them you will earn a referral fee. The position we have been engaged to work on is an ASCP Certified histotech with dermpath experience (Mohs is a plus and my client will train). This is for a BRAND NEW LAB located in the Dallas/Fort Worth area. This person will be the sole practitioner histotech in this brand new lab. You will be responsible for managing and maintaining the lab and performing histology. My client offers a competitive salary, nice benefits and an outstanding opportunity. For more information please contact me Pam Barker at relia1 at earthlink.net or toll free at 866-607-3542. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://urldefense.proofpoint.com/v2/url?u=http-3A__www.facebook.com&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=omI8jOva6UKQqxKwH4gzp2UJdX_bT8xmEUeQ_G1syr0&e= /PamBarkerRELIA https://urldefense.proofpoint.com/v2/url?u=http-3A__www.linkedin.com_in_reliasolutions&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=xiH78mZ72WPCDelZIdOYYgNAQvKqupggtDAawDR0SoI&e= https://urldefense.proofpoint.com/v2/url?u=http-3A__www.twitter.com_pamatrelia&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=rRJrxjCQ8hQu18AxFOkCy4kVvuUvBm089Ebnco9CqRI&e= ------------------------------ Message: 10 Date: Tue, 12 May 2015 16:26:58 +0000 From: Joelle Weaver To: "Roy, Lisa" , Paula Sicurello , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] H&E Stainer Question Message-ID: Content-Type: text/plain; charset="iso-8859-1" Personally I love the Prisma for volume and the tape. I know many have bad opinions, but I wish I had both the Prisma and the tape right now! I have never seen any problems with very old ( 15+ year) slides. using the tape. Not saying it can't happen-but have not personally seen it. The tape is easier to get off if you need to versus old glass CS, just use acetone, acetone/xylene, xylene. Comes off in a gel form and slides right off leaving the tissue intact. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Royl1 at LabCorp.com > To: patpxs at gmail.com; histonet at lists.utsouthwestern.edu > Date: Tue, 12 May 2015 12:18:46 +0000 > Subject: Re: [Histonet] H&E Stainer Question > > Paula > Here are my two cents.... > > I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. > > On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.....no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. > > Hope it helps....good luck. > Lisa > > -----Original Message----- > From: Paula Sicurello [mailto:patpxs at gmail.com] > Sent: Monday, May 11, 2015 5:04 PM > To: HistoNet > Subject: [Histonet] H&E Stainer Question > > Me again... > > UCSD is in the market for a new H&E stainer for our new hospital opening next year. > > We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. > > What do you use? > > Suggestions gratefully accepted-even from you two Keith and Matt ;) > > Opinions about the good, the bad, and the ugly (as long as it works really > well) will be helpful. > > Thanks oodles! > > Paula :-) > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=ojqKLAQtVhO8ixOrdIb13M0riQkWk2GB5BRQSgbZ3_Q&e= > -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=ojqKLAQtVhO8ixOrdIb13M0riQkWk2GB5BRQSgbZ3_Q&e= ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=ojqKLAQtVhO8ixOrdIb13M0riQkWk2GB5BRQSgbZ3_Q&e= ------------------------------ End of Histonet Digest, Vol 138, Issue 14 ***************************************** From tbraud at holyredeemer.com Tue May 12 12:11:48 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 12 May 2015 17:11:48 +0000 Subject: [Histonet] H&E STAINER In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F8010C81@HRHEX02-HOS.holyredeemer.local> Our Sakura Prisma stainer with the Sakura Glas coverslipper has been awesome for over 4 years. Very fast, easy maintenance, very forgiving of tech mistakes (not that we ever make any, lol) I'm not saying that it's better than Leica, just that we've worked it like a dog and have been very happy. Terri Braud Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 4. H&E Stainer Question (Paula Sicurello) Date: Mon, 11 May 2015 14:03:40 -0700 From: Paula Sicurello Subject: [Histonet] H&E Stainer Question UCSD is in the market for a new H&E stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) ******************************** From mjones at metropath.com Tue May 12 12:15:31 2015 From: mjones at metropath.com (Michael Ann Jones) Date: Tue, 12 May 2015 17:15:31 +0000 Subject: [Histonet] H&E STAINER Message-ID: Ditto! (except we have tape) Michael Ann On 5/12/15, 11:11 AM, "Terri Braud" wrote: >Our Sakura Prisma stainer with the Sakura Glas coverslipper has been >awesome for over 4 years. Very fast, easy maintenance, very forgiving of >tech mistakes (not that we ever make any, lol) I'm not saying that it's >better than Leica, just that we've worked it like a dog and have been >very happy. Terri Braud >Terri L. Braud, HT(ASCP) >Anatomic Pathology Supervisor >Holy Redeemer Hospital Laboratory >1648 Huntingdon Pike >Meadowbrook, PA 19046 >Ph: 215-938-3676 >Fax: 215-938-3874 > > 4. H&E Stainer Question (Paula Sicurello) >Date: Mon, 11 May 2015 14:03:40 -0700 >From: Paula Sicurello >Subject: [Histonet] H&E Stainer Question >UCSD is in the market for a new H&E stainer for our new hospital opening >next year. >We need a workhorse, not a prima dona, something with a coverslipper >built in would be nice. >What do you use? >Suggestions gratefully accepted-even from you two Keith and Matt ;) >Opinions about the good, the bad, and the ugly (as long as it works really >well) will be helpful. >Thanks oodles! >Paula :-) > >******************************** > > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpjones at srhs-pa.org Tue May 12 13:47:21 2015 From: lpjones at srhs-pa.org (Jones, Laura) Date: Tue, 12 May 2015 14:47:21 -0400 Subject: [Histonet] Formalin in the Operating Room/Department Message-ID: Histonet Experts, A little over a year ago, our hospital was bought by CHS. There have been many changes, but after visits from corporate advisors, it was decided that no formalin could be poured in the O.R./ department. Previously, they kept the large cubitainers of formalin in between the O.R. rooms in smaller utility rooms and accessed it from there. Now, smaller specimens are places in prefilled formalin vials, and larger specimens are supposed to be brought to us immediately so that we can put formalin on them. As you'd expect, we are finding specimens that have been sitting too long without fixative. The O.R. staff has been told that in our absence, they are to come to the lab and take care of this. Also, the hospital labor and delivery department cannot have formalin for specimens now. My questions are these: - Does anyone else have this situation? (Especially if you are a CHS hospital) And if you do, how do you make it work? - How is it safer to have small (up to 400 ml) prefilled jars of formalin in the O.R. or a nearby utility room? - What do you all think about the costs involved? Prefilled jars are not inexpensive. Maybe after 27 years I'm too resistant to change, but I see the potential for big problems. We have to do as we are instructed, but I'm interested in your expert opinions. Thanks in advance. Laura Jones B.A., HT, PBT (ASCP) | Lead Tech, Histology | Community Health Systems 740 East State Street | Sharon PA | Phone (724)983-3950 | Fax (724)983-3982 www.sharonregional.com | lpjones at srhs-pa.org ________________________________ Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Kathy.Machado at LPNT.net Tue May 12 13:50:32 2015 From: Kathy.Machado at LPNT.net (Kathy.Machado at LPNT.net) Date: Tue, 12 May 2015 18:50:32 +0000 Subject: [Histonet] : Re: H&E Stainer Question Message-ID: We just got rid of our Leica XL. It was not working right after about 10 years, little things started going wrong. And then it just quit "reading" the white clip that we used for counterstaining IHC. The cost for someone to come to the lab to fix it was ridiculous. The customer service from Leica was not the best. We now have a Gemini from Thermo and love it. It has two ovens and stains similar to the Leica. So far we have not had any problems and we LOVE our Thermo/Fisher reps. They have been wonderful. Kathy Machado, HTL Danville Regional Medical Center Danville, VA Kathy.Machado at lpnt.net 434-799-3867 From abtdhu at gmail.com Tue May 12 13:51:27 2015 From: abtdhu at gmail.com (Dorothy Hu) Date: Tue, 12 May 2015 14:51:27 -0400 Subject: [Histonet] TRAP Stain Help Message-ID: If you include a positive slide to your TRAP? It is a must for each run. It could be many things if you didn't have positive slide, will be hard to troubleshot. Also if you see any pink color befor counterstain/coversliping? If do, there may be because of decolored by ethanol/xylene and mounting media. You have to use water based mounting media. I don't use Sigma kit (tried once), but my protocol is more sensitive. We do trap on both paraffin and MMA sections. If you need I can forward you the paper. Dorothy Hu HSDM Message: 2 Date: Mon, 11 May 2015 20:26:14 +0000 From: "Wait, Trevor Jordan" To: "Histonet at Lists. Edu" Subject: [Histonet] TRAP Stain Help Message-ID: <1431375976414.44600 at livemail.uthscsa.edu> Content-Type: text/plain; charset="iso-8859-1" Hey guys, recently I've used the Sigma Aldrich TRAP Stain Kit in order to stain for Osteoclasts in Formalin Fixed Paraffin Embedded bone tissues that are EDTA decalcified. Unfortunately there was no TRAP stain to be found whatsoever on all slides that I stained. I was hoping that someone with some experience with TRAP stain could really help me out! Here are a couple of reasons that I have wondered as to why the TRAP stain might not be visible.... 1. Staining too long with Hematoxylin counterstain? I have noticed in several trial runs that sometimes if the Hematoxylin counterstain is too long then this can effect the amount of TRAP stain that shows up with the osteoclasts. Perhaps I'm just over analyzing.... 2. Perhaps no osteoclasts were present to even stain....does anyone know what the usual ratio of Osteoclasts to Osteocytes are? In the past stains that I have used....whenever there is an osteoclasts that shows up...it is usually pretty spotty and many times they are in groups together...is this how it normally is? 3. Perhaps the TRAP stain is being washed away through the rinsing with dI or dehydration with ethanol/clearing just before mounting. A protocol that I used before the Sigma Aldrich Kit incorporates incubating the slides in 37 celsius water for 1 hour just prior to allowing the slides to sit in the TRAP stain solution (this is also 37 celsius in the same water bath) and letting it sit for 20 minutes. With this stain...there seems to be a consistent showing of osteoclasts but I'm just not sure if all of the osteoclasts are showing up correctly...that is why I moved to the Sigma Aldrich kit to make sure and the results didn't show ANY osteoclasts there! Anyways...I'm just curious if there are any reasons that the TRAP stain is not showing up...I would appreciate any input! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate From DKBoyd at chs.net Tue May 12 14:25:33 2015 From: DKBoyd at chs.net (Boyd, Debbie M) Date: Tue, 12 May 2015 19:25:33 +0000 Subject: [Histonet] Formalin in the Operating Room/Department In-Reply-To: References: Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9F15C35@TN001WEXMBX014.US.chs.net> Hi Laura, The problem with pouring formalin in the OR is exposure to the fumes unless they have proper ventilation or a hood. Formalin spills are also a large risk especially for untrained staff. Air quality and personnel are checked annually for exposure, which is a Joint Commission and CAP requirement. We too are a CHS hospital but we changed that practice a long time ago. We have prefilled containers, 20 ml, and 120 ml. We also have empty containers 480 ml, 2 liter. and 5 liter. We fill the larger containers. We keep a containers in Histology of all sizes so OR attendants can pick them up as needed. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Jones, Laura [lpjones at srhs-pa.org] Sent: Tuesday, May 12, 2015 2:47 PM To: Histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] Formalin in the Operating Room/Department Histonet Experts, A little over a year ago, our hospital was bought by CHS. There have been many changes, but after visits from corporate advisors, it was decided that no formalin could be poured in the O.R./ department. Previously, they kept the large cubitainers of formalin in between the O.R. rooms in smaller utility rooms and accessed it from there. Now, smaller specimens are places in prefilled formalin vials, and larger specimens are supposed to be brought to us immediately so that we can put formalin on them. As you'd expect, we are finding specimens that have been sitting too long without fixative. The O.R. staff has been told that in our absence, they are to come to the lab and take care of this. Also, the hospital labor and delivery department cannot have formalin for specimens now. My questions are these: - Does anyone else have this situation? (Especially if you are a CHS hospital) And if you do, how do you make it work? - How is it safer to have small (up to 400 ml) prefilled jars of formalin in the O.R. or a nearby utility room? - What do you all think about the costs involved? Prefilled jars are not inexpensive. Maybe after 27 years I'm too resistant to change, but I see the potential for big problems. We have to do as we are instructed, but I'm interested in your expert opinions. Thanks in advance. Laura Jones B.A., HT, PBT (ASCP) | Lead Tech, Histology | Community Health Systems 740 East State Street | Sharon PA | Phone (724)983-3950 | Fax (724)983-3982 www.sharonregional.com | lpjones at srhs-pa.org ________________________________ Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From mjones at metropath.com Tue May 12 14:55:52 2015 From: mjones at metropath.com (Michael Ann Jones) Date: Tue, 12 May 2015 19:55:52 +0000 Subject: [Histonet] assigning pathologists cases for satellite labs Message-ID: Hello out in Histoland! I have questions regarding the triaging of case slides for delivery to satellite facilities where pathologists will interpret slides. Our LIS system is available at all sites for ordering deepers/special stains/IHC, etc. so we have that covered. We currently do not have digital pathology (yet!) so slides must be delivered to several hospital sites where pathologists are working. Can you all help with thoughts regarding pre-assigning pathologists to cases so that everyone in the lab knows where those slides are supposed to go? We've never assigned pathologists before, it was always first come, first served. How do you all deliver (in a timely fashion) cases to satellite facilities and determine who gets what for general work vs specialists (dermatopathologists)? It gets pretty chaotic back in our lab trying to figure out who gets what and where are they today. . . Oh, and they all want the slides by 7 a.m. ;) Thank you in advance for the advice- Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com From wdesalvo.cac at outlook.com Tue May 12 19:10:46 2015 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Tue, 12 May 2015 17:10:46 -0700 Subject: [Histonet] H&E Stainer Question In-Reply-To: <6295CCF61B0DBB4C9248F51F8940E6A10118F112@MSXMBXNSPRD09.acct.upmchs.net> References: , , <6295CCF61B0DBB4C9248F51F8940E6A10118F112@MSXMBXNSPRD09.acct.upmchs.net> Message-ID: Look at all the automated H&E stain instruments on the market. I suggest that you consider those that offer the best benefit to your workload and workflow. Instruments that utilize the concept of co-location (related tasks linked together; oven fro drying, flexible stain configuration and Coverslipping) will assist you in developing a LEAN workflow. Consider how many times you need to touch the slides to complete all the tasks and how much walk away time you gain. I do not suggest by-passing the validated staining instrument oven. Placing slides in another oven creates variation and often results in short and extended drying times. All automated H&E stain instruments should be used according to manufacturer recommendation. My experience is that when shortcuts are used, quality suffers. The automated H&E stain instruments have great through put and you should adjust workflow to maximize the designated batch size and stain time. Film coverslip lasts longer than 7 years. Sakura film is the best and has testing to exceed 10 years. I have used it for 13 years and never had the film peel. If you use the knock off film products, they only have short term accelerated stability testing and probably do not have any version of their many changes to the emulsion that have real time stability testing to exceed 10 years. There are reasons to use glass and reasons to use film. Both are great products, when you use them correctly and purchase quality products. Glass automated cover slip options on instruments do have more required maintenance than film. CAP has made no statement about film cover slipping. In fact, the Hologic (was Cytech) Cytology Thinprep system is FDA approved with film as the cover slip and the stained and film cover slipped Thinprep slide is digitally scanned for analysis. To be CAP compliant, you must keep blocks and slides for 10 years. With the advancement of cancer hospital protocols and molecular testing, many institutions are considering retaining blocks and slides beyond 10 years. Keeping blocks and slides longer than regulation requires introduces a large risk factor for the retaining institution and pathologists. Always be forward thinking when considering the purchase of a new essential instrument. Will it bar code read, can it be interfaced to LIS or tracking system, what analytics can be extract and will the instrument help or hinder a LEAN workflow? There are many choices that will meet your basic needs, but which one meets your essential needs? William DeSalvo, BS HTL(ASCP) > From: simmca at UPMC.EDU > To: Royl1 at LabCorp.com; patpxs at gmail.com; histonet at lists.utsouthwestern.edu > Date: Tue, 12 May 2015 12:32:43 +0000 > Subject: Re: [Histonet] H&E Stainer Question > > To be fair, a "batch" of slides for Leica is actually 270- slides, It can run 9 racks at a time, but, the 9th rack from start to coverslip is 3 hours+ > You can always skip the on-board oven and place your slides in a slide dryer (most labs have them) and then every 3 minutes you can load a rack (1st xylene step 3 minutes) > Then it goes much faster. > As for tape..ugh..it is only guaranteed to last 7 years, after that they pull off the slide and take the tissue with it. > CAP is starting to frown on this as you need to keep the initial H&E slides for up to 10+ years. > > Chris Simmons B.S., A.S., HTL(ASCP) > Supervisor, UPP Dermatopathology > 412.864.3880 office > 412.612.0881 cell > > > -----Original Message----- > From: Roy, Lisa [mailto:Royl1 at LabCorp.com] > Sent: Tuesday, May 12, 2015 8:19 AM > To: Paula Sicurello; HistoNet > Subject: Re: [Histonet] H&E Stainer Question > > Paula > Here are my two cents.... > > I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. > > On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.....no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. > > Hope it helps....good luck. > Lisa > > -----Original Message----- > From: Paula Sicurello [mailto:patpxs at gmail.com] > Sent: Monday, May 11, 2015 5:04 PM > To: HistoNet > Subject: [Histonet] H&E Stainer Question > > Me again... > > UCSD is in the market for a new H&E stainer for our new hospital opening next year. > > We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. > > What do you use? > > Suggestions gratefully accepted-even from you two Keith and Matt ;) > > Opinions about the good, the bad, and the ugly (as long as it works really > well) will be helpful. > > Thanks oodles! > > Paula :-) > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 at comcast.net Tue May 12 20:30:54 2015 From: suetp918 at comcast.net (Sue) Date: Wed, 13 May 2015 01:30:54 +0000 (UTC) Subject: [Histonet] H&E Stainer Question In-Reply-To: References: <6295CCF61B0DBB4C9248F51F8940E6A10118F112@MSXMBXNSPRD09.acct.upmchs.net> Message-ID: <634289525.910257.1431480654972.JavaMail.zimbra@comcast.net> I agree with William tape from anyone else but Sakura is not worth the time and effort. We were just CAP inspected and they said nothing about tape. I have had issues with tape and usually just cut around the sample on the tape and recover on a slide, works beautifully. As far as the oven time is essential and we do use an outside oven but validated using it. I agree to purchase a Leica with an attached cover slipper better lean approach. Stainers that use proprietary reagents bring costs up and in this day and age hospitals re putting us on a tighter budget. Just my 2 cents S paturzo From joost.bruijntjes at tno.triskelion.nl Wed May 13 07:52:09 2015 From: joost.bruijntjes at tno.triskelion.nl (Bruijntjes, J.P. (Joost)) Date: Wed, 13 May 2015 12:52:09 +0000 Subject: [Histonet] CD4-CD8-CD68 Message-ID: Hi all Is anyone of you familiar with the possibility of applying CD4, CD8 and CD68 antibodies on formalin fixed paraffin embedded liver of mice? Thanks in advance. Joost Bruijntjes From CDavis at che-east.org Wed May 13 08:25:32 2015 From: CDavis at che-east.org (Davis, Cassie) Date: Wed, 13 May 2015 09:25:32 -0400 Subject: [Histonet] Oceanic and Amphibian necropsy Message-ID: Good Morning Histoland, Does anybody out there have a good processing schedule for shark and frog necropsies? We are trying to help out a colleague who just started this study and needs a starting point. Thanks in advance for any help! Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington, DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From histology81176 at att.net Wed May 13 11:06:22 2015 From: histology81176 at att.net (Histology Technician) Date: Wed, 13 May 2015 16:06:22 +0000 (UTC) Subject: [Histonet] : Re: H&E Stainer Question In-Reply-To: References: Message-ID: <1721891248.597468.1431533182433.JavaMail.yahoo@mail.yahoo.com> Does anyone have a Thermo PrintMate that you'd like to share the SOP on?? Thanks! On Tuesday, May 12, 2015 1:50 PM, "Kathy.Machado at LPNT.net" wrote: We just got rid of our Leica XL.? It was not working right after about 10 years, little things started going wrong. And then it just quit "reading" the white clip that we used for counterstaining IHC.? The cost for someone to come to the lab to fix it was ridiculous.? The customer service from Leica was not the best. We now have a Gemini from Thermo and love it.? It has two ovens and stains similar to the Leica.? So far we have not had any problems and we LOVE our Thermo/Fisher reps.? They have been wonderful. Kathy Machado, HTL Danville Regional Medical Center Danville, VA Kathy.Machado at lpnt.net 434-799-3867 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From simmca at UPMC.EDU Wed May 13 11:18:42 2015 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Wed, 13 May 2015 16:18:42 +0000 Subject: [Histonet] : Re: H&E Stainer Question In-Reply-To: <1721891248.597468.1431533182433.JavaMail.yahoo@mail.yahoo.com> References: <1721891248.597468.1431533182433.JavaMail.yahoo@mail.yahoo.com> Message-ID: <6295CCF61B0DBB4C9248F51F8940E6A1011911A1@MSXMBXNSPRD09.acct.upmchs.net> Depending on your LIS integration, the block barcode is scanned, there is a delay that you can specify, then the slide(s) ordered on that particular block are printed in the order they are ordered. SlideMates have 2 versions, one sends a text file to the server to print the slide, the other sends a picture file that the slidemate copies, you will have to determine which one you want. They are a great time-saver, cost-saver, and CAP compliant machine. Chris Simmons B.S., A.S., HTL(ASCP) Supervisor, UPP Dermatopathology 412.864.3880 office 412.612.0881 cell -----Original Message----- From: Histology Technician [mailto:histology81176 at att.net] Sent: Wednesday, May 13, 2015 12:06 PM To: Kathy.Machado at LPNT.net; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] : Re: H&E Stainer Question Does anyone have a Thermo PrintMate that you'd like to share the SOP on?? Thanks! On Tuesday, May 12, 2015 1:50 PM, "Kathy.Machado at LPNT.net" wrote: We just got rid of our Leica XL.? It was not working right after about 10 years, little things started going wrong. And then it just quit "reading" the white clip that we used for counterstaining IHC.? The cost for someone to come to the lab to fix it was ridiculous.? The customer service from Leica was not the best. We now have a Gemini from Thermo and love it.? It has two ovens and stains similar to the Leica.? So far we have not had any problems and we LOVE our Thermo/Fisher reps.? They have been wonderful. Kathy Machado, HTL Danville Regional Medical Center Danville, VA Kathy.Machado at lpnt.net 434-799-3867 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BradleyJay at UH.ORG Wed May 13 11:51:57 2015 From: BradleyJay at UH.ORG (Bradley Jay) Date: Wed, 13 May 2015 12:51:57 -0400 Subject: [Histonet] cpt codes for quantitative and semi quantitative ihc Message-ID: From histology81176 at att.net Wed May 13 11:52:24 2015 From: histology81176 at att.net (Histology Technician) Date: Wed, 13 May 2015 16:52:24 +0000 (UTC) Subject: [Histonet] assigning pathologists cases for satellite labs In-Reply-To: References: Message-ID: <1920668374.650443.1431535944153.JavaMail.yahoo@mail.yahoo.com> Does anyone have a Medica Evoqua that you'd like to share the SOP?? I just got it and don't know much about it yet.?Thanks! On Tuesday, May 12, 2015 2:56 PM, Michael Ann Jones wrote: Hello out in Histoland! I have questions regarding the triaging of case slides for delivery to satellite facilities where pathologists will interpret slides. Our LIS system is available at all sites for ordering deepers/special stains/IHC, etc. so we have that covered. We currently do not have digital pathology (yet!) so slides must be delivered to several hospital sites where pathologists are working. Can you all help with thoughts regarding pre-assigning pathologists to cases so that everyone in the lab knows where those slides are supposed to go? We've never assigned pathologists before, it was always first come, first served. How do you all deliver (in a timely fashion) cases to satellite facilities and determine who gets what for general work vs specialists (dermatopathologists)? It gets pretty chaotic back in our lab trying to figure out who gets what and where are they today. . . Oh, and they all want the slides by 7 a.m. ;) Thank you in advance for the advice- Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MHuth at baymedical.org Wed May 13 12:04:15 2015 From: MHuth at baymedical.org (Myra Huth) Date: Wed, 13 May 2015 17:04:15 +0000 Subject: [Histonet] Histonet Digest, Vol 138, Issue 16 In-Reply-To: Message-ID: <6B3280E0CD2C394894743E076DC66C59E387@BMCMSEXCHMB2.corp.baymedical.org> Is anyone else only receiving blank pages from histonet? Thanks -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Wednesday, May 13, 2015 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 138, Issue 16 Confidentiality Notice: This email message and any attachments from Bay Medical Center Sacred Heart Health System, its subsidiaries and affiliates, are confidential and for the sole use of the intended recipient. This communication may contain privileged, proprietary, or confidential information (i.e., including Protected Health Information), which may only be used or disclosed in accordance with applicable law. If you are not the intended recipient of this email or the employee or agent responsible for delivering the communication to the intended recipient, then you may not read, copy, distribute or otherwise use or disclose the information contained in this message. If you received this message in error, please notify us by an email to Postmaster at baymedical.org. Please indicate that you were not the intended recipient, and confirm that you have deleted the original message and any attachments as well as any printed copies. Please do not retransmit the contents of the message. Thank you. From Toni.Rathborne at rwjuh.edu Wed May 13 12:07:28 2015 From: Toni.Rathborne at rwjuh.edu (Rathborne, Toni) Date: Wed, 13 May 2015 17:07:28 +0000 Subject: [Histonet] Histonet Digest, Vol 138, Issue 16 In-Reply-To: <6B3280E0CD2C394894743E076DC66C59E387@BMCMSEXCHMB2.corp.baymedical.org> References: <6B3280E0CD2C394894743E076DC66C59E387@BMCMSEXCHMB2.corp.baymedical.org> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F2401A2E9AF@vap1014.win.rwjuh.edu> Yes, the last one ([Histonet] cpt codes for quantitative and semi quantitative ihc )had no content. -----Original Message----- From: Myra Huth [mailto:MHuth at baymedical.org] Sent: Wednesday, May 13, 2015 1:04 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Histonet Digest, Vol 138, Issue 16 Is anyone else only receiving blank pages from histonet? Thanks -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Wednesday, May 13, 2015 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 138, Issue 16 Confidentiality Notice: This email message and any attachments from Bay Medical Center Sacred Heart Health System, its subsidiaries and affiliates, are confidential and for the sole use of the intended recipient. This communication may contain privileged, proprietary, or confidential information (i.e., including Protected Health Information), which may only be used or disclosed in accordance with applicable law. If you are not the intended recipient of this email or the employee or agent responsible for delivering the communication to the intended recipient, then you may not read, copy, distribute or otherwise use or disclose the information contained in this message. If you received this message in error, please notify us by an email to Postmaster at baymedical.org. Please indicate that you were not the intended recipient, and confirm that you have deleted the original message and any attachments as well as any printed copies. Please do not retransmit the contents of the message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis at bresnan.net Wed May 13 12:16:35 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Wed, 13 May 2015 11:16:35 -0600 Subject: [Histonet] Staining net dishes for brain sections Message-ID: <000201d08da0$90cf4190$b26dc4b0$@bresnan.net> Out of curiosity and having received an email from Brain Research Laboratories about this new item, has anyone tried the staining net dishes for free floating brain or other tissue sections? I thought this a clever, useful device to keep the sections from physical damage without having to lift the sections in some way. Gayle Callis HTL/HT/MT(ASCP) From gayle.callis at bresnan.net Wed May 13 12:34:01 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Wed, 13 May 2015 11:34:01 -0600 Subject: [Histonet] RE..... murine CD4, CD8 and CD68 for FFPE tissue Message-ID: <000701d08da3$00b34a40$0219dec0$@bresnan.net> You wrote: Is anyone of you familiar with the possibility of applying CD4, CD8 and CD68 antibodies on formalin fixed paraffin embedded liver of mice? Thanks in advance. Joost Bruijntjes ************************************** Thankfully and at long last after years of frustration by many ................ eBioscience has both the CD8a and CD4 which is now available for FFPE murine tissues. Read the data sheets carefully as the CD4 indicated this antibody was to be used on FFPE and not frozen sections. Anti-Mouse CD8a Purified Catalog Number: 14-0808 Also known as: CD8 alpha, Ly-2, Ly-35, Lyt-2 Anti-Mouse CD4 Purified Catalog Number: 14-9766 Also known as: L3T4, Ly-4 Serotec has a rat anti mouse CD68 where the data sheet says can be used on FFPE murine tissue. This clone must be available from other companies as well. Gayle M. Callis HTL/HT/MT(ASCP) From tina.vanmeter at gmail.com Wed May 13 12:42:27 2015 From: tina.vanmeter at gmail.com (Tina Van Meter) Date: Wed, 13 May 2015 13:42:27 -0400 Subject: [Histonet] Staining net dishes for brain sections In-Reply-To: <000201d08da0$90cf4190$b26dc4b0$@bresnan.net> References: <000201d08da0$90cf4190$b26dc4b0$@bresnan.net> Message-ID: Gayle, I purchased mine a few years ago from Electron Microscope Sciences (EMS) a few years ago. They work really well. It is easier on the tissue integrity and allows you to easily transfer from one solution to another. Tina Van Meter On May 13, 2015 1:16 PM, "Gayle Callis" wrote: > Out of curiosity and having received an email from Brain Research > Laboratories about this new item, has anyone tried the staining net dishes > for free floating brain or other tissue sections? > > > > I thought this a clever, useful device to keep the sections from physical > damage without having to lift the sections in some way. > > > > Gayle Callis > > HTL/HT/MT(ASCP) > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tbraud at holyredeemer.com Wed May 13 12:57:55 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 13 May 2015 17:57:55 +0000 Subject: [Histonet] In Defense of Tape In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F801109A@HRHEX02-HOS.holyredeemer.local> I've been using Tape coverslipping for over 20 years. I've seen the problems with the tape lifting the section off of the slide, HOWEVER, there is a specific technical solution to keep this from occurring. Lifting occurs because of inadequate dehydration before Xylene clearing to coverslip. Xylene is very forgiving of minute amounts of water carried over that might be microscopically undetectable, but Tape is not. Any minute amount of water carried into the xylene before tape coverslipping will cause lifting. I have 20 y/o slides coverslipped with tape with no issues. I have heard that tape covered slides can't be scanned, but I've not confirmed this with a reliable source. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 From simmca at UPMC.EDU Wed May 13 13:09:22 2015 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Wed, 13 May 2015 18:09:22 +0000 Subject: [Histonet] In Defense of Tape In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F801109A@HRHEX02-HOS.holyredeemer.local> References: , <48E053DDF6CE074DB6A7414BA05403F801109A@HRHEX02-HOS.holyredeemer.local> Message-ID: As someone who has scanned thousands of slides that are both tape and glass on a digital platform I can tell you that glass is the gold standard. Tape does scan decently, but the larger issue is that tape cover slips are too easily damaged during manual handling. The small scratches make digital image quality suboptimal. Glass is highly resistant to these minor issues but not immune. Digital image scanning for primary diagnosis is still a long way away so no need to jump the gun now, but it is coming, and it will revolutionize our diagnostic delivery systems. Be ready! Sent from my iPhone > On May 13, 2015, at 1:58 PM, Terri Braud wrote: > > I've been using Tape coverslipping for over 20 years. I've seen the problems with the tape lifting the section off of the slide, HOWEVER, there is a specific technical solution to keep this from occurring. Lifting occurs because of inadequate dehydration before Xylene clearing to coverslip. Xylene is very forgiving of minute amounts of water carried over that might be microscopically undetectable, but Tape is not. Any minute amount of water carried into the xylene before tape coverslipping will cause lifting. > I have 20 y/o slides coverslipped with tape with no issues. I have heard that tape covered slides can't be scanned, but I've not confirmed this with a reliable source. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hardin at oncology.wisc.edu Wed May 13 13:12:49 2015 From: hardin at oncology.wisc.edu (Joseph Hardin) Date: Wed, 13 May 2015 18:12:49 +0000 Subject: [Histonet] RE..... murine CD4, CD8 and CD68 for FFPE tissue In-Reply-To: <000701d08da3$00b34a40$0219dec0$@bresnan.net> References: <000701d08da3$00b34a40$0219dec0$@bresnan.net> Message-ID: <1431540771819.68363@oncology.wisc.edu> Has anyone tried these Abs? If so, please send me your beautiful photos and protocol. Joseph Hardin Senior Research Specialist UWCCC Experimental Pathology WIMR I Rm. 4012 1111 Highland Av. Madison, WI 53705 (608)262-1836 ________________________________________ From: Gayle Callis Sent: Wednesday, May 13, 2015 12:34 PM To: Histonet Subject: [Histonet] RE..... murine CD4, CD8 and CD68 for FFPE tissue You wrote: Is anyone of you familiar with the possibility of applying CD4, CD8 and CD68 antibodies on formalin fixed paraffin embedded liver of mice? Thanks in advance. Joost Bruijntjes ************************************** Thankfully and at long last after years of frustration by many ................ eBioscience has both the CD8a and CD4 which is now available for FFPE murine tissues. Read the data sheets carefully as the CD4 indicated this antibody was to be used on FFPE and not frozen sections. Anti-Mouse CD8a Purified Catalog Number: 14-0808 Also known as: CD8 alpha, Ly-2, Ly-35, Lyt-2 Anti-Mouse CD4 Purified Catalog Number: 14-9766 Also known as: L3T4, Ly-4 Serotec has a rat anti mouse CD68 where the data sheet says can be used on FFPE murine tissue. This clone must be available from other companies as well. Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 at cdc.gov Wed May 13 13:18:53 2015 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Wed, 13 May 2015 18:18:53 +0000 Subject: [Histonet] In Defense of Tape In-Reply-To: References: , <48E053DDF6CE074DB6A7414BA05403F801109A@HRHEX02-HOS.holyredeemer.local> Message-ID: <3B2CD438E1628A41BD687E98B963B78137F3ACBE@EMBX-CLFT4.cdc.gov> One word: telepathology! -----Original Message----- From: Simmons, Christopher [mailto:simmca at UPMC.EDU] Sent: Wednesday, May 13, 2015 2:09 PM To: Terri Braud Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] In Defense of Tape As someone who has scanned thousands of slides that are both tape and glass on a digital platform I can tell you that glass is the gold standard. Tape does scan decently, but the larger issue is that tape cover slips are too easily damaged during manual handling. The small scratches make digital image quality suboptimal. Glass is highly resistant to these minor issues but not immune. Digital image scanning for primary diagnosis is still a long way away so no need to jump the gun now, but it is coming, and it will revolutionize our diagnostic delivery systems. Be ready! Sent from my iPhone > On May 13, 2015, at 1:58 PM, Terri Braud wrote: > > I've been using Tape coverslipping for over 20 years. I've seen the problems with the tape lifting the section off of the slide, HOWEVER, there is a specific technical solution to keep this from occurring. Lifting occurs because of inadequate dehydration before Xylene clearing to coverslip. Xylene is very forgiving of minute amounts of water carried over that might be microscopically undetectable, but Tape is not. Any minute amount of water carried into the xylene before tape coverslipping will cause lifting. > I have 20 y/o slides coverslipped with tape with no issues. I have heard that tape covered slides can't be scanned, but I've not confirmed this with a reliable source. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katherine-walters at uiowa.edu Wed May 13 13:26:43 2015 From: katherine-walters at uiowa.edu (Walters, Katherine S) Date: Wed, 13 May 2015 18:26:43 +0000 Subject: [Histonet] Staining net dishes for brain sections In-Reply-To: <000201d08da0$90cf4190$b26dc4b0$@bresnan.net> References: <000201d08da0$90cf4190$b26dc4b0$@bresnan.net> Message-ID: <8F5174AF8E55114E8D36E4024D8B02C41A864495@HC-MAILBOXC1-N6.healthcare.uiowa.edu> Yes, I love them! -----Original Message----- From: Gayle Callis [mailto:gayle.callis at bresnan.net] Sent: Wednesday, May 13, 2015 12:17 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Staining net dishes for brain sections Out of curiosity and having received an email from Brain Research Laboratories about this new item, has anyone tried the staining net dishes for free floating brain or other tissue sections? I thought this a clever, useful device to keep the sections from physical damage without having to lift the sections in some way. Gayle Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ From Timothy.Morken at ucsf.edu Wed May 13 13:41:07 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 13 May 2015 18:41:07 +0000 Subject: [Histonet] scanning with tape RE: In Defense of Tape Message-ID: <761E2B5697F795489C8710BCC72141FF368319D7@ex07.net.ucsf.edu> FYI, We are manually coverslipping frozen slides with tape, due to the fact it dries very fast, and then scanning immediately for remote review. It is working very well. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Terri Braud [mailto:tbraud at holyredeemer.com] Sent: Wednesday, May 13, 2015 10:58 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] In Defense of Tape I've been using Tape coverslipping for over 20 years. I've seen the problems with the tape lifting the section off of the slide, HOWEVER, there is a specific technical solution to keep this from occurring. Lifting occurs because of inadequate dehydration before Xylene clearing to coverslip. Xylene is very forgiving of minute amounts of water carried over that might be microscopically undetectable, but Tape is not. Any minute amount of water carried into the xylene before tape coverslipping will cause lifting. I have 20 y/o slides coverslipped with tape with no issues. I have heard that tape covered slides can't be scanned, but I've not confirmed this with a reliable source. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa at alliedsearchpartners.com Wed May 13 14:46:46 2015 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Wed, 13 May 2015 19:46:46 +0000 Subject: [Histonet] Day Shift Histology Supervisor Job in NC for Direct Hire Message-ID: Hello All, I have a Direct Hire opportunity offering relocation assistance in Greensboro, NC. This position is a Histology Supervisor job on the Day Shift. Full Time/Direct Hire. Please reach out if you would like to view my job description and apply. To view a complete list of Allied Search Partners current openings go to: http://www.jobs.net/jobs/alliedsearchpartners/en-us/all-jobs/United-States/ Melissa Owens (Phelan) President, Laboratory Staffing Allied Search Partners www.linkedin.com/in/melissaphelan/ http://www.alliedsearchpartners.com T: 888.388.7571 ext. 102 F: 888.388.7572 From mjdessoye at commonwealthhealth.net Thu May 14 06:43:53 2015 From: mjdessoye at commonwealthhealth.net (Dessoye, Michael) Date: Thu, 14 May 2015 11:43:53 +0000 Subject: [Histonet] OJT Histotechs/Training Message-ID: Hello Histonet, I'm curious how people are dealing with on-the-job-trained histotechs. Many people are seeing a shortage in techs, and in my opinion OJT will become more common than it already is. Does anyone have an 'official' training program? Requirements to pass the exam? Qualifications to be able to be trained on-the-job? I'd like to consider having some kind of plan in place when I don't have an HT/HTL applicant but have folks who, if they get the experience, are otherwise qualified to sit for the exam. If anyone has a similar situation or experience to share I would appreciate it! Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Royl1 at LabCorp.com Thu May 14 07:55:19 2015 From: Royl1 at LabCorp.com (Roy, Lisa) Date: Thu, 14 May 2015 12:55:19 +0000 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: References: Message-ID: I currently have 3 open tech positions and don't have any qualified applicants applying for the job. I have recently taken a lab aide that showed interest and aptitude and began OJT. With less than 30 schools in the country actually teaching histology, this is one day going to be the way. Already having a bachelors in biology, my aide qualifies to sit for the ASCP exam once he has completed one full year of tech work and has a pathologist willing to review his work and sign off on the ASCP paperwork. Without going through a traditional program, one must have an associates or bachelor's degree with a certain amount of Chemistry and Science credits. As far as the training, I started with embedding and moved on from there to cutting and then special staining. All along way, working on troubleshooting and documenting EVERYTHING. Some places will hire someone with only a high school diploma as long as they have previous HT experience. I think the specifics of what each institution would deem a qualified trainee will vary from place to place. Smaller hospitals or labs may be okay training someone with aptitude that doesn't necessarily fit the ASCP exam qualifications, but large corporations might really insist that the trainee be certifiable at some point. Frankly, I think taking someone that shows an interest and has the knowledge to be a great tech is better than hiring someone that you may not know what you are getting. Doing OJT ensures that you are teaching the candidate exactly how you want things done and not having to accept the bad habits of someone that has been doing it a long time and set in their own ways. Good luck Lisa -----Original Message----- From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] Sent: Thursday, May 14, 2015 7:44 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] OJT Histotechs/Training Hello Histonet, I'm curious how people are dealing with on-the-job-trained histotechs. Many people are seeing a shortage in techs, and in my opinion OJT will become more common than it already is. Does anyone have an 'official' training program? Requirements to pass the exam? Qualifications to be able to be trained on-the-job? I'd like to consider having some kind of plan in place when I don't have an HT/HTL applicant but have folks who, if they get the experience, are otherwise qualified to sit for the exam. If anyone has a similar situation or experience to share I would appreciate it! Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. From ecameron1 at midcoasthealth.com Thu May 14 09:07:44 2015 From: ecameron1 at midcoasthealth.com (Cameron, Elizabeth) Date: Thu, 14 May 2015 14:07:44 +0000 Subject: [Histonet] Sakura Film Coverslipper Message-ID: Just wondering if anyone using Sakura film coverslippers has noticed streaks along the edges of the slides. I am sure they are caused by the adhesive leaking out and being wiped down the slide when we wipe the xylene off, but I am wondering if anyone has any suggestions for eliminating the streaks. We have tried letting the slides air dry and still get "waterspots" where the xylene was. Thanks. Elizabeth M. Cameron, HT (ASCP), QIHC Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 From mucram11 at comcast.net Thu May 14 11:17:55 2015 From: mucram11 at comcast.net (Pam Marcum) Date: Thu, 14 May 2015 16:17:55 +0000 (UTC) Subject: [Histonet] OJT Histotechs/Training In-Reply-To: References: Message-ID: <1171438095.3265261.1431620275731.JavaMail.zimbra@comcast.net> I understand and agree with everything being said and feel we do need more education in getting your registry, as Histology is changing and growing.??We need to be prepared to grow with it, much as we did when IHC first came into Histology and many thought it would go to the MTs.?? ? The one thing that has not changed in the 50 years I have done Histology is the fact that no one outside of AP knows what a Histologist is or what we do.? (I'm tried of being asked "Oh what kind of history is that?")? Until we change that and get more information about the field and advantages we will still be in the straights we are in now.? No one joining because so few people even know what we do or that there is an opportunity here.? If you don't know what Histology is why would you even look at the field.? I know about and have done school visits, career days etc; and those are not enough.? ? We need a spokesperson or celebrity?who has needed our services and not even known we, Histology, were the ones who did the slides their wonderful doctors used to save their lives.? This person or persons needs to speak loud and strong the way Robin Roberts has done on TV for her doctors and?help.?However; Histology was neven mentioned in those gratis moments.?I have only known one?person in NSH who suggested this and no one listened.? If?they can't see you or know you - you don't exist.??Can we all take off the blinders and?look at what we need in publicity and stop waiting for NSH and ASCP to do it.???Then we can offer these possible future HTs and HTLs something, like being recognized as full laboratory professionals and a higher level of lab aide. ? Just my thoughts (for many years and spoken often) ? Pam Marcum ----- Original Message ----- From: "Lisa Roy" To: "Michael Dessoye" , "Histonet" Sent: Thursday, May 14, 2015 7:55:19 AM Subject: Re: [Histonet] OJT Histotechs/Training I currently have 3 open tech positions and don't have any qualified applicants applying for the job. ?I have recently taken a lab aide that showed interest and aptitude and began OJT. ?With less than 30 schools in the country actually teaching histology, this is one day going to be the way. ?Already having a bachelors in biology, my aide qualifies to sit for the ASCP exam once he has completed one full year of tech work and has a pathologist willing to review his work and sign off on the ASCP paperwork. ?Without going through a traditional program, one must have an associates or bachelor's degree with a ?certain amount of Chemistry and Science credits. ?As far as the training, I started with embedding and moved on from there to cutting and then special staining. ?All along way, working on troubleshooting and documenting EVERYTHING. ?Some places will hire someone with only a high school diploma as long as they have previous HT experience. ?I think the specifics of what each institution would deem a qualified trainee will vary from place to place. ?Smaller hospitals or labs may be okay training someone with aptitude that doesn't necessarily fit the ASCP exam qualifications, but large corporations might really insist that the trainee be certifiable at some point. Frankly, I think taking someone that shows an interest and has the knowledge to be a great tech is better than hiring someone that you may not know what you are getting. ?Doing OJT ensures that you are teaching the candidate exactly how you want things done and not having to accept the bad habits of someone that has been doing it a long time and set in their own ways. Good luck Lisa ? ? -----Original Message----- From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] Sent: Thursday, May 14, 2015 7:44 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] OJT Histotechs/Training Hello Histonet, I'm curious how people are dealing with on-the-job-trained histotechs. ?Many people are seeing a shortage in techs, and in my opinion OJT will become more common than it already is. ?Does anyone have an 'official' training program? ?Requirements to pass the exam? ?Qualifications to be able to be trained on-the-job? ?I'd like to consider having some kind of plan in place when I don't have an HT/HTL applicant but have folks who, if they get the experience, are otherwise qualified to sit for the exam. ?If anyone has a similar situation or experience to share I would appreciate it! Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA at mercer.edu Thu May 14 11:59:37 2015 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Thu, 14 May 2015 12:59:37 -0400 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: <1171438095.3265261.1431620275731.JavaMail.zimbra@comcast.net> References: <1171438095.3265261.1431620275731.JavaMail.zimbra@comcast.net> Message-ID: <9BF995BC0E47744E9673A41486E24EE25C02BAA603@MERCERMAIL.MercerU.local> Oh where is the "LIKE" button Pam? Thanks, and that is the reason NSH and state societies were begun, to educate the public about our existence and that we are very valuable to them. Shirley, soon to be 53 years in the shadows. -----Original Message----- From: Pam Marcum [mailto:mucram11 at comcast.net] Sent: Thursday, May 14, 2015 12:18 PM To: Lisa Roy Cc: Histonet; Michael Dessoye Subject: Re: [Histonet] OJT Histotechs/Training I understand and agree with everything being said and feel we do need more education in getting your registry, as Histology is changing and growing.??We need to be prepared to grow with it, much as we did when IHC first came into Histology and many thought it would go to the MTs.?? ? The one thing that has not changed in the 50 years I have done Histology is the fact that no one outside of AP knows what a Histologist is or what we do.? (I'm tried of being asked "Oh what kind of history is that?")? Until we change that and get more information about the field and advantages we will still be in the straights we are in now.? No one joining because so few people even know what we do or that there is an opportunity here.? If you don't know what Histology is why would you even look at the field.? I know about and have done school visits, career days etc; and those are not enough.? ? We need a spokesperson or celebrity?who has needed our services and not even known we, Histology, were the ones who did the slides their wonderful doctors used to save their lives.? This person or persons needs to speak loud and strong the way Robin Roberts has done on TV for her doctors and?help.?However; Histology was neven mentioned in those gratis moments.?I have only known one?person in NSH who suggested this and no one listened.? If?they can't see you or know you - you don't exist.??Can we all take off the blinders and?look at what we need in publicity and stop waiting for NSH and ASCP to do it.???Then we can offer these possible future HTs and HTLs something, like being recognized as full laboratory professionals and a higher level of lab aide. ? Just my thoughts (for many years and spoken often) ? Pam Marcum ----- Original Message ----- From: "Lisa Roy" To: "Michael Dessoye" , "Histonet" Sent: Thursday, May 14, 2015 7:55:19 AM Subject: Re: [Histonet] OJT Histotechs/Training I currently have 3 open tech positions and don't have any qualified applicants applying for the job. ?I have recently taken a lab aide that showed interest and aptitude and began OJT. ?With less than 30 schools in the country actually teaching histology, this is one day going to be the way. ?Already having a bachelors in biology, my aide qualifies to sit for the ASCP exam once he has completed one full year of tech work and has a pathologist willing to review his work and sign off on the ASCP paperwork. ?Without going through a traditional program, one must have an associates or bachelor's degree with a ?certain amount of Chemistry and Science credits. ?As far as the training, I started with embedding and moved on from there to cutting and then special staining. ?All along way, working on troubleshooting and documenting EVERYTHING. ?Some places will hire someone with only a high school diploma as long as they have previous HT experience. ?I think the specifics of what each institution would deem a qualified trainee will vary from place to place. ?Smaller hospitals or labs may be okay training someone with aptitude that doesn't necessarily fit the ASCP exam qualifications, but large corporations might really insist that the trainee be certifiable at some point. Frankly, I think taking someone that shows an interest and has the knowledge to be a great tech is better than hiring someone that you may not know what you are getting. ?Doing OJT ensures that you are teaching the candidate exactly how you want things done and not having to accept the bad habits of someone that has been doing it a long time and set in their own ways. Good luck Lisa ? ? -----Original Message----- From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] Sent: Thursday, May 14, 2015 7:44 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] OJT Histotechs/Training Hello Histonet, I'm curious how people are dealing with on-the-job-trained histotechs. ?Many people are seeing a shortage in techs, and in my opinion OJT will become more common than it already is. ?Does anyone have an 'official' training program? ?Requirements to pass the exam? ?Qualifications to be able to be trained on-the-job? ?I'd like to consider having some kind of plan in place when I don't have an HT/HTL applicant but have folks who, if they get the experience, are otherwise qualified to sit for the exam. ?If anyone has a similar situation or experience to share I would appreciate it! Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Thu May 14 12:07:06 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 14 May 2015 17:07:06 +0000 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: <1171438095.3265261.1431620275731.JavaMail.zimbra@comcast.net> References: <1171438095.3265261.1431620275731.JavaMail.zimbra@comcast.net> Message-ID: <761E2B5697F795489C8710BCC72141FF36831E99@ex07.net.ucsf.edu> I think there is some actor from the CSI series that has done some of this work promoting lab techs... Tim Morken -----Original Message----- From: Pam Marcum [mailto:mucram11 at comcast.net] Sent: Thursday, May 14, 2015 9:18 AM To: Lisa Roy Cc: Histonet; Michael Dessoye Subject: Re: [Histonet] OJT Histotechs/Training I understand and agree with everything being said and feel we do need more education in getting your registry, as Histology is changing and growing.??We need to be prepared to grow with it, much as we did when IHC first came into Histology and many thought it would go to the MTs.?? ? The one thing that has not changed in the 50 years I have done Histology is the fact that no one outside of AP knows what a Histologist is or what we do.? (I'm tried of being asked "Oh what kind of history is that?")? Until we change that and get more information about the field and advantages we will still be in the straights we are in now.? No one joining because so few people even know what we do or that there is an opportunity here.? If you don't know what Histology is why would you even look at the field.? I know about and have done school visits, career days etc; and those are not enough.? ? We need a spokesperson or celebrity?who has needed our services and not even known we, Histology, were the ones who did the slides their wonderful doctors used to save their lives.? This person or persons needs to speak loud and strong the way Robin Roberts has done on TV for her doctors and?help.?However; Histology was neven mentioned in those gratis moments.?I have only known one?person in NSH who suggested this and no one listened.? If?they can't see you or know you - you don't exist.??Can we all take off the blinders and?look at what we need in publicity and stop waiting for NSH and ASCP to do it.???Then we can offer these possible future HTs and HTLs something, like being recognized as full laboratory professionals and a higher level of lab aide. ? Just my thoughts (for many years and spoken often) ? Pam Marcum ----- Original Message ----- From: "Lisa Roy" To: "Michael Dessoye" , "Histonet" Sent: Thursday, May 14, 2015 7:55:19 AM Subject: Re: [Histonet] OJT Histotechs/Training I currently have 3 open tech positions and don't have any qualified applicants applying for the job. ?I have recently taken a lab aide that showed interest and aptitude and began OJT. ?With less than 30 schools in the country actually teaching histology, this is one day going to be the way. ?Already having a bachelors in biology, my aide qualifies to sit for the ASCP exam once he has completed one full year of tech work and has a pathologist willing to review his work and sign off on the ASCP paperwork. ?Without going through a traditional program, one must have an associates or bachelor's degree with a ?certain amount of Chemistry and Science credits. ?As far as the training, I started with embedding and moved on from there to cutting and then special staining. ?All along way, working on troubleshooting and documenting EVERYTHING. ?Some places will hire someone with only a high school diploma as long as they have previous HT experience. ?I think the specifics of what each institution would deem a qualified trainee will vary from place to place. ?Smaller hospitals or labs may be okay training someone with aptitude that doesn't necessarily fit the ASCP exam qualifications, but large corporations might really insist that the trainee be certifiable at some point. Frankly, I think taking someone that shows an interest and has the knowledge to be a great tech is better than hiring someone that you may not know what you are getting. ?Doing OJT ensures that you are teaching the candidate exactly how you want things done and not having to accept the bad habits of someone that has been doing it a long time and set in their own ways. Good luck Lisa ? ? -----Original Message----- From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] Sent: Thursday, May 14, 2015 7:44 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] OJT Histotechs/Training Hello Histonet, I'm curious how people are dealing with on-the-job-trained histotechs. ?Many people are seeing a shortage in techs, and in my opinion OJT will become more common than it already is. ?Does anyone have an 'official' training program? ?Requirements to pass the exam? ?Qualifications to be able to be trained on-the-job? ?I'd like to consider having some kind of plan in place when I don't have an HT/HTL applicant but have folks who, if they get the experience, are otherwise qualified to sit for the exam. ?If anyone has a similar situation or experience to share I would appreciate it! Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From smokkapa at mdanderson.org Thu May 14 12:19:02 2015 From: smokkapa at mdanderson.org (Mokkapati,Sharada) Date: Thu, 14 May 2015 17:19:02 +0000 Subject: [Histonet] TCF21 antibody staining on human tissue Message-ID: Hi all, Does anyone here have experience in working with TCF21 antibody for IHC on FFPE human sections? Any suggestion will be valuable. Thanks Sharada From langleykristi at hotmail.com Thu May 14 12:20:46 2015 From: langleykristi at hotmail.com (Kristi Langley) Date: Thu, 14 May 2015 12:20:46 -0500 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25C02BAA603@MERCERMAIL.MercerU.local> References: , , <1171438095.3265261.1431620275731.JavaMail.zimbra@comcast.net>, <9BF995BC0E47744E9673A41486E24EE25C02BAA603@MERCERMAIL.MercerU.local> Message-ID: I am currently an ASCP certified traveling home grown HT. lol....I have to say, that until the organizations recognize histo as a PROFESSION we are drowning. I have been placed in places where the pay was phenominal! My current placement pays barely above minimun wage for HT. To give you a better understanding, the phlebs that answer the tube system and deliver blood to the appropriate departments, make more than their histo techs. None of them in my department, including the supervisor are certified and they produce EXCELLENT work period. I have also worked w 4 yr degreed people that I seriously shake my head at....lol.....we all have the struggle....just my 2 cents > From: POWELL_SA at mercer.edu > To: mucram11 at comcast.net; Royl1 at LabCorp.com > Date: Thu, 14 May 2015 12:59:37 -0400 > Subject: Re: [Histonet] OJT Histotechs/Training > CC: histonet at lists.utsouthwestern.edu; mjdessoye at commonwealthhealth.net > > Oh where is the "LIKE" button Pam? Thanks, and that is the reason NSH and state societies were begun, to educate the public about our existence and that we are very valuable to them. > > Shirley, soon to be 53 years in the shadows. > > > -----Original Message----- > From: Pam Marcum [mailto:mucram11 at comcast.net] > Sent: Thursday, May 14, 2015 12:18 PM > To: Lisa Roy > Cc: Histonet; Michael Dessoye > Subject: Re: [Histonet] OJT Histotechs/Training > > I understand and agree with everything being said and feel we do need more education in getting your registry, as Histology is changing and growing. We need to be prepared to grow with it, much as we did when IHC first came into Histology and many thought it would go to the MTs. > > The one thing that has not changed in the 50 years I have done Histology is the fact that no one outside of AP knows what a Histologist is or what we do. (I'm tried of being asked "Oh what kind of history is that?") Until we change that and get more information about the field and advantages we will still be in the straights we are in now. No one joining because so few people even know what we do or that there is an opportunity here. If you don't know what Histology is why would you even look at the field. I know about and have done school visits, career days etc; and those are not enough. > > We need a spokesperson or celebrity who has needed our services and not even known we, Histology, were the ones who did the slides their wonderful doctors used to save their lives. This person or persons needs to speak loud and strong the way Robin Roberts has done on TV for her doctors and help. However; Histology was neven mentioned in those gratis moments. I have only known one person in NSH who suggested this and no one listened. If they can't see you or know you - you don't exist. Can we all take off the blinders and look at what we need in publicity and stop waiting for NSH and ASCP to do it. Then we can offer these possible future HTs and HTLs something, like being recognized as full laboratory professionals and a higher level of lab aide. > > Just my thoughts (for many years and spoken often) > > Pam Marcum > > ----- Original Message ----- > > From: "Lisa Roy" > To: "Michael Dessoye" , "Histonet" > Sent: Thursday, May 14, 2015 7:55:19 AM > Subject: Re: [Histonet] OJT Histotechs/Training > > I currently have 3 open tech positions and don't have any qualified applicants applying for the job. I have recently taken a lab aide that showed interest and aptitude and began OJT. With less than 30 schools in the country actually teaching histology, this is one day going to be the way. Already having a bachelors in biology, my aide qualifies to sit for the ASCP exam once he has completed one full year of tech work and has a pathologist willing to review his work and sign off on the ASCP paperwork. Without going through a traditional program, one must have an associates or bachelor's degree with a certain amount of Chemistry and Science credits. As far as the training, I started with embedding and moved on from there to cutting and then special staining. All along way, working on troubleshooting and documenting EVERYTHING. Some places will hire someone with only a high school diploma as long as they have previous HT experience. I think the specifics of what each institution would deem a qualified trainee will vary from place to place. Smaller hospitals or labs may be okay training someone with aptitude that doesn't necessarily fit the ASCP exam qualifications, but large corporations might really insist that the trainee be certifiable at some point. > > Frankly, I think taking someone that shows an interest and has the knowledge to be a great tech is better than hiring someone that you may not know what you are getting. Doing OJT ensures that you are teaching the candidate exactly how you want things done and not having to accept the bad habits of someone that has been doing it a long time and set in their own ways. > > Good luck > Lisa > > -----Original Message----- > From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] > Sent: Thursday, May 14, 2015 7:44 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] OJT Histotechs/Training > > Hello Histonet, > > I'm curious how people are dealing with on-the-job-trained histotechs. Many people are seeing a shortage in techs, and in my opinion OJT will become more common than it already is. Does anyone have an 'official' training program? Requirements to pass the exam? Qualifications to be able to be trained on-the-job? I'd like to consider having some kind of plan in place when I don't have an HT/HTL applicant but have folks who, if they get the experience, are otherwise qualified to sit for the exam. If anyone has a similar situation or experience to share I would appreciate it! > > Thanks, > Mike > > Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA at mercer.edu Thu May 14 12:27:29 2015 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Thu, 14 May 2015 13:27:29 -0400 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: <761E2B5697F795489C8710BCC72141FF36831E99@ex07.net.ucsf.edu> References: <1171438095.3265261.1431620275731.JavaMail.zimbra@comcast.net> <761E2B5697F795489C8710BCC72141FF36831E99@ex07.net.ucsf.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE25C02BAA63B@MERCERMAIL.MercerU.local> William Peterson? -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Thursday, May 14, 2015 1:07 PM To: Pam Marcum; Lisa Roy Cc: Histonet; Michael Dessoye Subject: Re: [Histonet] OJT Histotechs/Training I think there is some actor from the CSI series that has done some of this work promoting lab techs... Tim Morken -----Original Message----- From: Pam Marcum [mailto:mucram11 at comcast.net] Sent: Thursday, May 14, 2015 9:18 AM To: Lisa Roy Cc: Histonet; Michael Dessoye Subject: Re: [Histonet] OJT Histotechs/Training I understand and agree with everything being said and feel we do need more education in getting your registry, as Histology is changing and growing.??We need to be prepared to grow with it, much as we did when IHC first came into Histology and many thought it would go to the MTs.?? ? The one thing that has not changed in the 50 years I have done Histology is the fact that no one outside of AP knows what a Histologist is or what we do.? (I'm tried of being asked "Oh what kind of history is that?")? Until we change that and get more information about the field and advantages we will still be in the straights we are in now.? No one joining because so few people even know what we do or that there is an opportunity here.? If you don't know what Histology is why would you even look at the field.? I know about and have done school visits, career days etc; and those are not enough.? ? We need a spokesperson or celebrity?who has needed our services and not even known we, Histology, were the ones who did the slides their wonderful doctors used to save their lives.? This person or persons needs to speak loud and strong the way Robin Roberts has done on TV for her doctors and?help.?However; Histology was neven mentioned in those gratis moments.?I have only known one?person in NSH who suggested this and no one listened.? If?they can't see you or know you - you don't exist.??Can we all take off the blinders and?look at what we need in publicity and stop waiting for NSH and ASCP to do it.???Then we can offer these possible future HTs and HTLs something, like being recognized as full laboratory professionals and a higher level of lab aide. ? Just my thoughts (for many years and spoken often) ? Pam Marcum ----- Original Message ----- From: "Lisa Roy" To: "Michael Dessoye" , "Histonet" Sent: Thursday, May 14, 2015 7:55:19 AM Subject: Re: [Histonet] OJT Histotechs/Training I currently have 3 open tech positions and don't have any qualified applicants applying for the job. ?I have recently taken a lab aide that showed interest and aptitude and began OJT. ?With less than 30 schools in the country actually teaching histology, this is one day going to be the way. ?Already having a bachelors in biology, my aide qualifies to sit for the ASCP exam once he has completed one full year of tech work and has a pathologist willing to review his work and sign off on the ASCP paperwork. ?Without going through a traditional program, one must have an associates or bachelor's degree with a ?certain amount of Chemistry and Science credits. ?As far as the training, I started with embedding and moved on from there to cutting and then special staining. ?All along way, working on troubleshooting and documenting EVERYTHING. ?Some places will hire someone with only a high school diploma as long as they have previous HT experience. ?I think the specifics of what each institution would deem a qualified trainee will vary from place to place. ?Smaller hospitals or labs may be okay training someone with aptitude that doesn't necessarily fit the ASCP exam qualifications, but large corporations might really insist that the trainee be certifiable at some point. Frankly, I think taking someone that shows an interest and has the knowledge to be a great tech is better than hiring someone that you may not know what you are getting. ?Doing OJT ensures that you are teaching the candidate exactly how you want things done and not having to accept the bad habits of someone that has been doing it a long time and set in their own ways. Good luck Lisa ? ? -----Original Message----- From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] Sent: Thursday, May 14, 2015 7:44 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] OJT Histotechs/Training Hello Histonet, I'm curious how people are dealing with on-the-job-trained histotechs. ?Many people are seeing a shortage in techs, and in my opinion OJT will become more common than it already is. ?Does anyone have an 'official' training program? ?Requirements to pass the exam? ?Qualifications to be able to be trained on-the-job? ?I'd like to consider having some kind of plan in place when I don't have an HT/HTL applicant but have folks who, if they get the experience, are otherwise qualified to sit for the exam. ?If anyone has a similar situation or experience to share I would appreciate it! Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Thu May 14 12:28:15 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 14 May 2015 17:28:15 +0000 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF36831EC5@ex07.net.ucsf.edu> Mike, yes, the vast majority of histotechs have been, are, and will be OJT (me included). The people who take on training these people have a responsibility to do the best they can. Most techs end up learning whatever their lab does and so have limited knowledge. I studied a full year for the HT and passed fine, and later the HTL. In our small lab at the time we had a broad array of testing in histology (specials, muscle histochem, immunochem, electron microscopy), but I found out my true lack of knowledge when I went to Saudi Arabia and worked with techs from other countries where they had comprehensive bachelors-level programs required for ALL lab techs. Those from the US, all certificated, where vastly under-educated compared to techs from other countries. It was a bit embarrassing! Luckily we have online courses and degrees available now - not available in the 1980's when I started. That is a tremendous advantage to those who are willing to take advantage of it. Other than that it will be up to the lab management to be sure the OJT tech gets the basic instruction according to the requirements of the ASCP exam. That is the bare bones knowledge necessary to function. Even then the experience in the lab is key to whether the knowledge is just regurgitated or practiced. Lab management has a responsibility to be sure good lab practices are ingrained during training. It is a big job. As an aside, there are some people out there trying to break into histology but do not work in a histo lab, or work in a lab that does not support their desire to get certificated (which is practically criminal in my view). I talked to a person recently who is working in a histo lab but is trying to find a lab to do special stains they do not do in the lab they are working in. Their lab will not buy them the reagents necessary and actually told this person that they will not help them get certificated because they feel the person will move on to get better pay elsewhere. I agree with another thought expressed that finding a person excited about getting into histology can lead to a good tech. I had a person just show up cold one day saying he really wanted to work in the histo lab - he had learned some histology in a research lab and did not realize it could be a full time profession until he stumbled on our lab one day. He had a good background but we had no histo jobs open, but we happened to have a new grossing lab aid job opening and he managed to get that job. The expectation is that he will eventually work his way into histology. He's happy to have his foot in the door, and we are happy to have an enthusiastic person with a plan for advancement. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] Sent: Thursday, May 14, 2015 4:44 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] OJT Histotechs/Training Hello Histonet, I'm curious how people are dealing with on-the-job-trained histotechs. Many people are seeing a shortage in techs, and in my opinion OJT will become more common than it already is. Does anyone have an 'official' training program? Requirements to pass the exam? Qualifications to be able to be trained on-the-job? I'd like to consider having some kind of plan in place when I don't have an HT/HTL applicant but have folks who, if they get the experience, are otherwise qualified to sit for the exam. If anyone has a similar situation or experience to share I would appreciate it! Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Audrey.Pagan at nyumc.org Thu May 14 12:42:10 2015 From: Audrey.Pagan at nyumc.org (Pagan, Audrey) Date: Thu, 14 May 2015 17:42:10 +0000 Subject: [Histonet] Job Openings at NYU Langone Medical Center Message-ID: <5B503E47B7641B4885238F3CA0F2EB9B44266D0F@MSGWCDCPMB24.nyumc.org> Hello All, NYU Langone Medical Center has 4 positions open in the Anatomic Pathology Department, 2 histology/IHC supervisors, 1 grossing assistant, and 1 pathology case coordinator. The following are brief descriptions of the positions. Please visit the NYU website http://careers.nyumc.org/ for more detailed information about each. If you are interested or have questions please contact me by email or apply online. Histology/IHC Supervisors: Supervises staff and workflow within the main anatomic pathology laboratory. Ensures proper performance of all techniques and procedures, ensures quality work and regulatory preparedness are met. Oversees all areas of the main laboratory including: histology, immunohistochemistry, immunofluorescence, in-situ hybridization, and Renal Pathology processing. Must have Bachelor's Degree in clinical laboratory science, medical technology, biology or related field, HT or HTL(ASCP) certification, NYSDOH license as Clinical Laboratory histotechnician, technician, or technologist. 6 years' experience in histology and IHC with 0-2 years supervisory experience. Job ID: 1024781_RR00004000 and 1024782_RR00004005 Grossing Assistant: responsible for the gross examination and dissection of pathology biopsy specimens. They prepare biopsy tissue for histological processing and prepare the gross descriptions and clinical history relevant for each biopsy case. Must have Bachelor's Degree in clinical laboratory science, medical technology, biology or related field. One year required experience in a surgical pathology laboratory including gross dissection and dictation of surgical biopsy specimens. NYSDOH License as a Clinical Laboratory histotechnician, technician, or technologist. Job ID: 1024424_RR00003638 Pathology Case Coordinator: Seeking an experienced Pathology lab technician/technologist to work on QA monitoring, issue resolution, error tracking, and departmental improvement projects. Must have a Bachelor's Degree with two years of experience as a laboratory technician or a technologist. NYSDOH license not required. Job ID: 1024670_RR00003912 From CDavis at che-east.org Thu May 14 13:34:12 2015 From: CDavis at che-east.org (Davis, Cassie) Date: Thu, 14 May 2015 14:34:12 -0400 Subject: [Histonet] Histology schools-OJT In-Reply-To: References: Message-ID: I know DTCC in Wilmington, DE has an accredited associates degree program since 1990 that graduates 5-6 students a year and they flood the area. Anybody hiring might want to contact the school, they need jobs! Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org ________________________________________ ***************************************** Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From kmilne at bccrc.ca Thu May 14 13:45:23 2015 From: kmilne at bccrc.ca (Katy Milne) Date: Thu, 14 May 2015 18:45:23 +0000 Subject: [Histonet] murine CD4, CD8 and CD68 for FFPE tissue In-Reply-To: References: Message-ID: Hi all, I tried to respond yesterday but forgot to cut off a bunch making the email too big but I can add to the comments below... First up, Gayle, thanks for the heads up that the CD8 is finally out, I'd heard it was happening and had been checking back but hadn't seen anything yet. Will get that ordered ASAP. Joseph I have used the CD4 and it looks pretty good so far, I'm not sure how to attach images on here but I can send you a stain although I've mostly been using it for multicolour stuff. I use all Biocare reagents on an Intellipath FLX for my staining so we could see if we could translate a protocol to whatever platform you're using. Joost, you can multiplex some of these Abs to get all your markers on one slide if you'd like. I have pooled the CD4 mentioned and CD3 SP7 clone in the first round (which generally highlights CD8s as single positives vs the double positive CD4/CD3s) and then put F4/80 in the second round as a mac marker in place of CD68. Didn't get back to fully fleshing it out yet but I also pooled PAX5 for B cells in there and got some nice images of tertiary lymphoid structures in mouse tissue. We're doing some more staining with it today for a different project. Anyway, CD4 Ab looks good so far, will order CD8 in for testing soon! Katy Message: 8 Date: Wed, 13 May 2015 18:12:49 +0000 From: Joseph Hardin To: Histonet , "gayle.callis at bresnan.net" Subject: Re: [Histonet] RE..... murine CD4, CD8 and CD68 for FFPE tissue Message-ID: <1431540771819.68363 at oncology.wisc.edu> Content-Type: text/plain; CHARSET=US-ASCII Has anyone tried these Abs? If so, please send me your beautiful photos and protocol. Joseph Hardin Senior Research Specialist UWCCC Experimental Pathology WIMR I Rm. 4012 1111 Highland Av. Madison, WI 53705 (608)262-1836 ________________________________________ From: Gayle Callis Sent: Wednesday, May 13, 2015 12:34 PM To: Histonet Subject: [Histonet] RE..... murine CD4, CD8 and CD68 for FFPE tissue You wrote: Is anyone of you familiar with the possibility of applying CD4, CD8 and CD68 antibodies on formalin fixed paraffin embedded liver of mice? Thanks in advance. Joost Bruijntjes ************************************** Thankfully and at long last after years of frustration by many ................ eBioscience has both the CD8a and CD4 which is now available for FFPE murine tissues. Read the data sheets carefully as the CD4 indicated this antibody was to be used on FFPE and not frozen sections. Anti-Mouse CD8a Purified Catalog Number: 14-0808 Also known as: CD8 alpha, Ly-2, Ly-35, Lyt-2 Anti-Mouse CD4 Purified Catalog Number: 14-9766 Also known as: L3T4, Ly-4 Serotec has a rat anti mouse CD68 where the data sheet says can be used on FFPE murine tissue. This clone must be available from other companies as well. Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cjohnson at nmda.nmsu.edu Thu May 14 13:51:19 2015 From: cjohnson at nmda.nmsu.edu (Johnson, Carole) Date: Thu, 14 May 2015 18:51:19 +0000 Subject: [Histonet] Rabbit-to-Rabbit IHC Message-ID: Has anyone had experience with the Rabbit-to-Rabbit Blocking Reagent from ScyTek? Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From jvickroy at SpringfieldClinic.com Thu May 14 14:25:37 2015 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Thu, 14 May 2015 19:25:37 +0000 Subject: [Histonet] Antimarkup in Illinois Message-ID: <9B1A1501A800064397369BD8072E6BCA063F226A@E2k10DB2.springfieldclinic.com> We have a new histology lab and we contract a pathology group to come to our facility and read slides. If they order a Special stain or IHC stain we send the slides to a hospital histology department to perform the stains. According to the new markup law we have to pass on and not markup the technical component of the stain, however since our contracted pathologist reads it here can we charge a usual and customary fee for reading the stain (professional component) which is higher than the negotiated fee that we pay the pathologist group for interpretation. Again the slides are read at our facility. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From jjcampbell14 at att.net Thu May 14 14:33:31 2015 From: jjcampbell14 at att.net (John & Jen Campbell) Date: Thu, 14 May 2015 12:33:31 -0700 Subject: [Histonet] TSA detection (during immunoflurorescence) on buffy coat slides Message-ID: <1431632011.63609.YahooMailBasic@web181603.mail.ne1.yahoo.com> Hi all, Does anyone have experience with multiple immunofluorescence on buffy coat slides? And further, has anyone used TSA detection when doing IF on buffy coats? I just wanted to know if there are any special considerations to take when using TSA detection during multiple labeling of buffy coats. Thanks in advance! Jennifer HT(ASCP)cm From joelleweaver at hotmail.com Thu May 14 14:35:10 2015 From: joelleweaver at hotmail.com (Joelle Weaver) Date: Thu, 14 May 2015 19:35:10 +0000 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: <761E2B5697F795489C8710BCC72141FF36831EC5@ex07.net.ucsf.edu> References: , <761E2B5697F795489C8710BCC72141FF36831EC5@ex07.net.ucsf.edu> Message-ID: We have discussed this on the histonet many times... Most professions, and most if not all healthcare professions, require degrees and/or certification for entry. This is how the public, other medical professions ,and even HR-who do not know the technical- assess for our capacity to provide care and judge the skill level needed of the profession as they are "looking in". We all know that this isn't always perhaps the best method to assess or measure some aspects of this profession, but this is what they work from. There are good and bad examples of both OJT and educated, formally trained histology professionals. However, "education" is more than learning facts, it helps develop many other facets of the person that are viewed as valuable to organizations. That is why it is used as a screening tool. Please try to value the broader perspective. Technical proficiency itself is probably not going to be enough as the future unfolds. Though it may seem unfair if you have worked for a very long time and learned a great deal through experience, the bottom line is that for some employers, some environments and outside groups- education, credentials and professionalism are the primary criteria they use to evaluate, and they pay and recognize accordingly. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken at ucsf.edu > To: histonet at lists.utsouthwestern.edu > Date: Thu, 14 May 2015 17:28:15 +0000 > Subject: Re: [Histonet] OJT Histotechs/Training > > Mike, yes, the vast majority of histotechs have been, are, and will be OJT (me included). The people who take on training these people have a responsibility to do the best they can. Most techs end up learning whatever their lab does and so have limited knowledge. I studied a full year for the HT and passed fine, and later the HTL. In our small lab at the time we had a broad array of testing in histology (specials, muscle histochem, immunochem, electron microscopy), but I found out my true lack of knowledge when I went to Saudi Arabia and worked with techs from other countries where they had comprehensive bachelors-level programs required for ALL lab techs. Those from the US, all certificated, where vastly under-educated compared to techs from other countries. It was a bit embarrassing! > > Luckily we have online courses and degrees available now - not available in the 1980's when I started. That is a tremendous advantage to those who are willing to take advantage of it. Other than that it will be up to the lab management to be sure the OJT tech gets the basic instruction according to the requirements of the ASCP exam. That is the bare bones knowledge necessary to function. Even then the experience in the lab is key to whether the knowledge is just regurgitated or practiced. Lab management has a responsibility to be sure good lab practices are ingrained during training. It is a big job. > > As an aside, there are some people out there trying to break into histology but do not work in a histo lab, or work in a lab that does not support their desire to get certificated (which is practically criminal in my view). I talked to a person recently who is working in a histo lab but is trying to find a lab to do special stains they do not do in the lab they are working in. Their lab will not buy them the reagents necessary and actually told this person that they will not help them get certificated because they feel the person will move on to get better pay elsewhere. > > I agree with another thought expressed that finding a person excited about getting into histology can lead to a good tech. I had a person just show up cold one day saying he really wanted to work in the histo lab - he had learned some histology in a research lab and did not realize it could be a full time profession until he stumbled on our lab one day. He had a good background but we had no histo jobs open, but we happened to have a new grossing lab aid job opening and he managed to get that job. The expectation is that he will eventually work his way into histology. He's happy to have his foot in the door, and we are happy to have an enthusiastic person with a plan for advancement. > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > -----Original Message----- > From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] > Sent: Thursday, May 14, 2015 4:44 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] OJT Histotechs/Training > > Hello Histonet, > > I'm curious how people are dealing with on-the-job-trained histotechs. Many people are seeing a shortage in techs, and in my opinion OJT will become more common than it already is. Does anyone have an 'official' training program? Requirements to pass the exam? Qualifications to be able to be trained on-the-job? I'd like to consider having some kind of plan in place when I don't have an HT/HTL applicant but have folks who, if they get the experience, are otherwise qualified to sit for the exam. If anyone has a similar situation or experience to share I would appreciate it! > > Thanks, > Mike > > Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV at archildrens.org Thu May 14 14:46:40 2015 From: HornHV at archildrens.org (Horn, Hazel V) Date: Thu, 14 May 2015 14:46:40 -0500 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: References: , <761E2B5697F795489C8710BCC72141FF36831EC5@ex07.net.ucsf.edu> Message-ID: <25A4DE08332B19499904459F00AAACB71A1EB25585@EVS1.archildrens.org> Joelle I agree with you. But the problem is, no one knows we exist. OJT is the only route for some/if not most positions to be filled. We would all love to have a choice of educated ASCP registered techs to choose from. I have an open position and no applicants. Hazel Horn, HTL/HT (ASCP) Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv at archildrens.org archildrens.org -----Original Message----- From: Joelle Weaver [mailto:joelleweaver at hotmail.com] Sent: Thursday, May 14, 2015 2:35 PM To: Morken, Timothy; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] OJT Histotechs/Training We have discussed this on the histonet many times... Most professions, and most if not all healthcare professions, require degrees and/or certification for entry. This is how the public, other medical professions ,and even HR-who do not know the technical- assess for our capacity to provide care and judge the skill level needed of the profession as they are "looking in". We all know that this isn't always perhaps the best method to assess or measure some aspects of this profession, but this is what they work from. There are good and bad examples of both OJT and educated, formally trained histology professionals. However, "education" is more than learning facts, it helps develop many other facets of the person that are viewed as valuable to organizations. That is why it is used as a screening tool. Please try to value the broader perspective. Technical proficiency itself is probably not going to be enough as the future unfolds. Though it may seem unfair if you have worked for a very long time and learned a great deal through experience, the bottom line is that for some employers, some environments and outside groups- education, credentials and professionalism are the primary criteria they use to evaluate, and they pay and recognize accordingly. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: Timothy.Morken at ucsf.edu > To: histonet at lists.utsouthwestern.edu > Date: Thu, 14 May 2015 17:28:15 +0000 > Subject: Re: [Histonet] OJT Histotechs/Training > > Mike, yes, the vast majority of histotechs have been, are, and will be OJT (me included). The people who take on training these people have a responsibility to do the best they can. Most techs end up learning whatever their lab does and so have limited knowledge. I studied a full year for the HT and passed fine, and later the HTL. In our small lab at the time we had a broad array of testing in histology (specials, muscle histochem, immunochem, electron microscopy), but I found out my true lack of knowledge when I went to Saudi Arabia and worked with techs from other countries where they had comprehensive bachelors-level programs required for ALL lab techs. Those from the US, all certificated, where vastly under-educated compared to techs from other countries. It was a bit embarrassing! > > Luckily we have online courses and degrees available now - not available in the 1980's when I started. That is a tremendous advantage to those who are willing to take advantage of it. Other than that it will be up to the lab management to be sure the OJT tech gets the basic instruction according to the requirements of the ASCP exam. That is the bare bones knowledge necessary to function. Even then the experience in the lab is key to whether the knowledge is just regurgitated or practiced. Lab management has a responsibility to be sure good lab practices are ingrained during training. It is a big job. > > As an aside, there are some people out there trying to break into histology but do not work in a histo lab, or work in a lab that does not support their desire to get certificated (which is practically criminal in my view). I talked to a person recently who is working in a histo lab but is trying to find a lab to do special stains they do not do in the lab they are working in. Their lab will not buy them the reagents necessary and actually told this person that they will not help them get certificated because they feel the person will move on to get better pay elsewhere. > > I agree with another thought expressed that finding a person excited about getting into histology can lead to a good tech. I had a person just show up cold one day saying he really wanted to work in the histo lab - he had learned some histology in a research lab and did not realize it could be a full time profession until he stumbled on our lab one day. He had a good background but we had no histo jobs open, but we happened to have a new grossing lab aid job opening and he managed to get that job. The expectation is that he will eventually work his way into histology. He's happy to have his foot in the door, and we are happy to have an enthusiastic person with a plan for advancement. > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology UC San Francisco Medical Center > > > -----Original Message----- > From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] > Sent: Thursday, May 14, 2015 4:44 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] OJT Histotechs/Training > > Hello Histonet, > > I'm curious how people are dealing with on-the-job-trained histotechs. Many people are seeing a shortage in techs, and in my opinion OJT will become more common than it already is. Does anyone have an 'official' training program? Requirements to pass the exam? Qualifications to be able to be trained on-the-job? I'd like to consider having some kind of plan in place when I don't have an HT/HTL applicant but have folks who, if they get the experience, are otherwise qualified to sit for the exam. If anyone has a similar situation or experience to share I would appreciate it! > > Thanks, > Mike > > Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | > Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | > mjdessoye at commonwealthhealth.net et> | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 > | Fax: 570-552-1486 > > > > ---------------------------------------------------------------------- > ---- > Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From joelleweaver at hotmail.com Thu May 14 15:26:15 2015 From: joelleweaver at hotmail.com (Joelle Weaver) Date: Thu, 14 May 2015 20:26:15 +0000 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: <25A4DE08332B19499904459F00AAACB71A1EB25585@EVS1.archildrens.org> References: , , <761E2B5697F795489C8710BCC72141FF36831EC5@ex07.net.ucsf.edu>, , <25A4DE08332B19499904459F00AAACB71A1EB25585@EVS1.archildrens.org> Message-ID: I know it is hard Hazel. I have a hard time finding well qualified people myself. OJT only works now if you already have a degree. If we can't raise the pay and the recognition we will have some difficulty recruiting those people too. I know the situation well, as I used to be the sole educator person in an HT program. It closed due to lack of enrollment and funding. I have trained people the best way that I knew how both formally and informally. I have driven in my car to multiple states,written articles, gone to conferences, schools, colleges and trade shows, and did my personal best to present topics and "sell' our profession. Admittedly, sometimes it feels like it doesn't matter or change anything. But we have to keep trying and pushing for our group, its best interests, its public recognition, its compensation. There will be no budging from anyone else I'm afraid. Change often moves like a glacier, but it does move! Joelle Weaver MAOM, HTL (ASCP) QIHC > From: HornHV at archildrens.org > To: joelleweaver at hotmail.com; timothy.morken at ucsf.edu; histonet at lists.utsouthwestern.edu > Date: Thu, 14 May 2015 14:46:40 -0500 > Subject: RE: [Histonet] OJT Histotechs/Training > > Joelle I agree with you. But the problem is, no one knows we exist. OJT is the only route for some/if not most positions to be filled. We would all love to have a choice of educated ASCP registered techs to choose from. I have an open position and no applicants. > > Hazel Horn, HTL/HT (ASCP) > Supervisor of Histology/Autopsy/Transcription > Anatomic Pathology > Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1241 fax > hornhv at archildrens.org > archildrens.org > > > > > > -----Original Message----- > From: Joelle Weaver [mailto:joelleweaver at hotmail.com] > Sent: Thursday, May 14, 2015 2:35 PM > To: Morken, Timothy; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] OJT Histotechs/Training > > We have discussed this on the histonet many times... > > Most professions, and most if not all healthcare professions, require degrees and/or certification for entry. This is how the public, other medical professions ,and even HR-who do not know the technical- assess for our capacity to provide care and judge the skill level needed of the profession as they are "looking in". We all know that this isn't always perhaps the best method to assess or measure some aspects of this profession, but this is what they work from. There are good and bad examples of both OJT and educated, formally trained histology professionals. However, "education" is more than learning facts, it helps develop many other facets of the person that are viewed as valuable to organizations. That is why it is used as a screening tool. Please try to value the broader perspective. Technical proficiency itself is probably not going to be enough as the future unfolds. Though it may seem unfair if you have worked for a very long time and learned a great deal through experience, the bottom line is that for some employers, some environments and outside groups- education, credentials and professionalism are the primary criteria they use to evaluate, and they pay and recognize accordingly. > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > From: Timothy.Morken at ucsf.edu > > To: histonet at lists.utsouthwestern.edu > > Date: Thu, 14 May 2015 17:28:15 +0000 > > Subject: Re: [Histonet] OJT Histotechs/Training > > > > Mike, yes, the vast majority of histotechs have been, are, and will be OJT (me included). The people who take on training these people have a responsibility to do the best they can. Most techs end up learning whatever their lab does and so have limited knowledge. I studied a full year for the HT and passed fine, and later the HTL. In our small lab at the time we had a broad array of testing in histology (specials, muscle histochem, immunochem, electron microscopy), but I found out my true lack of knowledge when I went to Saudi Arabia and worked with techs from other countries where they had comprehensive bachelors-level programs required for ALL lab techs. Those from the US, all certificated, where vastly under-educated compared to techs from other countries. It was a bit embarrassing! > > > > Luckily we have online courses and degrees available now - not available in the 1980's when I started. That is a tremendous advantage to those who are willing to take advantage of it. Other than that it will be up to the lab management to be sure the OJT tech gets the basic instruction according to the requirements of the ASCP exam. That is the bare bones knowledge necessary to function. Even then the experience in the lab is key to whether the knowledge is just regurgitated or practiced. Lab management has a responsibility to be sure good lab practices are ingrained during training. It is a big job. > > > > As an aside, there are some people out there trying to break into histology but do not work in a histo lab, or work in a lab that does not support their desire to get certificated (which is practically criminal in my view). I talked to a person recently who is working in a histo lab but is trying to find a lab to do special stains they do not do in the lab they are working in. Their lab will not buy them the reagents necessary and actually told this person that they will not help them get certificated because they feel the person will move on to get better pay elsewhere. > > > > I agree with another thought expressed that finding a person excited about getting into histology can lead to a good tech. I had a person just show up cold one day saying he really wanted to work in the histo lab - he had learned some histology in a research lab and did not realize it could be a full time profession until he stumbled on our lab one day. He had a good background but we had no histo jobs open, but we happened to have a new grossing lab aid job opening and he managed to get that job. The expectation is that he will eventually work his way into histology. He's happy to have his foot in the door, and we are happy to have an enthusiastic person with a plan for advancement. > > > > Tim Morken > > Pathology Site Manager, Parnassus > > Supervisor, Electron Microscopy/Neuromuscular Special Studies > > Department of Pathology UC San Francisco Medical Center > > > > > > -----Original Message----- > > From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] > > Sent: Thursday, May 14, 2015 4:44 AM > > To: histonet at lists.utsouthwestern.edu > > Subject: [Histonet] OJT Histotechs/Training > > > > Hello Histonet, > > > > I'm curious how people are dealing with on-the-job-trained histotechs. Many people are seeing a shortage in techs, and in my opinion OJT will become more common than it already is. Does anyone have an 'official' training program? Requirements to pass the exam? Qualifications to be able to be trained on-the-job? I'd like to consider having some kind of plan in place when I don't have an HT/HTL applicant but have folks who, if they get the experience, are otherwise qualified to sit for the exam. If anyone has a similar situation or experience to share I would appreciate it! > > > > Thanks, > > Mike > > > > Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | > > Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | > > mjdessoye at commonwealthhealth.net > et> | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 > > | Fax: 570-552-1486 > > > > > > > > ---------------------------------------------------------------------- > > ---- > > Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your computer. > Thank you. From hmarlatt26 at gmail.com Thu May 14 15:31:10 2015 From: hmarlatt26 at gmail.com (Heather Marlatt) Date: Thu, 14 May 2015 13:31:10 -0700 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF36831EC5@ex07.net.ucsf.edu> <25A4DE08332B19499904459F00AAACB71A1EB25585@EVS1.archildrens.org> Message-ID: I agree and would also like to "like" Pams response. On May 14, 2015 1:26 PM, "Joelle Weaver" wrote: > I know it is hard Hazel. I have a hard time finding well qualified people > myself. OJT only works now if you already have a degree. If we can't raise > the pay and the recognition we will have some difficulty recruiting those > people too. I know the situation well, as I used to be the sole educator > person in an HT program. It closed due to lack of enrollment and funding. I > have trained people the best way that I knew how both formally and > informally. I have driven in my car to multiple states,written articles, > gone to conferences, schools, colleges and trade shows, and did my > personal best to present topics and "sell' our profession. Admittedly, > sometimes it feels like it doesn't matter or change anything. But we have > to keep trying and pushing for our group, its best interests, its public > recognition, its compensation. There will be no budging from anyone else > I'm afraid. Change often moves like a glacier, but it does move! > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > From: HornHV at archildrens.org > > To: joelleweaver at hotmail.com; timothy.morken at ucsf.edu; > histonet at lists.utsouthwestern.edu > > Date: Thu, 14 May 2015 14:46:40 -0500 > > Subject: RE: [Histonet] OJT Histotechs/Training > > > > Joelle I agree with you. But the problem is, no one knows we exist. > OJT is the only route for some/if not most positions to be filled. We > would all love to have a choice of educated ASCP registered techs to choose > from. I have an open position and no applicants. > > > > Hazel Horn, HTL/HT (ASCP) > > Supervisor of Histology/Autopsy/Transcription > > Anatomic Pathology > > Arkansas Children's Hospital > > 1 Children's Way | Slot 820| Little Rock, AR 72202 > > 501.364.4240 direct | 501.364.1241 fax > > hornhv at archildrens.org > > archildrens.org > > > > > > > > > > > > -----Original Message----- > > From: Joelle Weaver [mailto:joelleweaver at hotmail.com] > > Sent: Thursday, May 14, 2015 2:35 PM > > To: Morken, Timothy; histonet at lists.utsouthwestern.edu > > Subject: Re: [Histonet] OJT Histotechs/Training > > > > We have discussed this on the histonet many times... > > > > Most professions, and most if not all healthcare professions, require > degrees and/or certification for entry. This is how the public, other > medical professions ,and even HR-who do not know the technical- assess for > our capacity to provide care and judge the skill level needed of the > profession as they are "looking in". We all know that this isn't always > perhaps the best method to assess or measure some aspects of this > profession, but this is what they work from. There are good and bad > examples of both OJT and educated, formally trained histology > professionals. However, "education" is more than learning facts, it helps > develop many other facets of the person that are viewed as valuable to > organizations. That is why it is used as a screening tool. Please try to > value the broader perspective. Technical proficiency itself is probably > not going to be enough as the future unfolds. Though it may seem unfair if > you have worked for a very long time and learned a great deal through > experience, the bottom line is that for some employers, some environments > and outside groups- education, credentials and professionalism are the > primary criteria they use to evaluate, and they pay and recognize > accordingly. > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > > > > > > > From: Timothy.Morken at ucsf.edu > > > To: histonet at lists.utsouthwestern.edu > > > Date: Thu, 14 May 2015 17:28:15 +0000 > > > Subject: Re: [Histonet] OJT Histotechs/Training > > > > > > Mike, yes, the vast majority of histotechs have been, are, and will > be OJT (me included). The people who take on training these people have a > responsibility to do the best they can. Most techs end up learning whatever > their lab does and so have limited knowledge. I studied a full year for the > HT and passed fine, and later the HTL. In our small lab at the time we had > a broad array of testing in histology (specials, muscle histochem, > immunochem, electron microscopy), but I found out my true lack of knowledge > when I went to Saudi Arabia and worked with techs from other countries > where they had comprehensive bachelors-level programs required for ALL lab > techs. Those from the US, all certificated, where vastly under-educated > compared to techs from other countries. It was a bit embarrassing! > > > > > > Luckily we have online courses and degrees available now - not > available in the 1980's when I started. That is a tremendous advantage to > those who are willing to take advantage of it. Other than that it will be > up to the lab management to be sure the OJT tech gets the basic instruction > according to the requirements of the ASCP exam. That is the bare bones > knowledge necessary to function. Even then the experience in the lab is key > to whether the knowledge is just regurgitated or practiced. Lab management > has a responsibility to be sure good lab practices are ingrained during > training. It is a big job. > > > > > > As an aside, there are some people out there trying to break into > histology but do not work in a histo lab, or work in a lab that does not > support their desire to get certificated (which is practically criminal in > my view). I talked to a person recently who is working in a histo lab but > is trying to find a lab to do special stains they do not do in the lab they > are working in. Their lab will not buy them the reagents necessary and > actually told this person that they will not help them get certificated > because they feel the person will move on to get better pay elsewhere. > > > > > > I agree with another thought expressed that finding a person excited > about getting into histology can lead to a good tech. I had a person just > show up cold one day saying he really wanted to work in the histo lab - he > had learned some histology in a research lab and did not realize it could > be a full time profession until he stumbled on our lab one day. He had a > good background but we had no histo jobs open, but we happened to have a > new grossing lab aid job opening and he managed to get that job. The > expectation is that he will eventually work his way into histology. He's > happy to have his foot in the door, and we are happy to have an > enthusiastic person with a plan for advancement. > > > > > > Tim Morken > > > Pathology Site Manager, Parnassus > > > Supervisor, Electron Microscopy/Neuromuscular Special Studies > > > Department of Pathology UC San Francisco Medical Center > > > > > > > > > -----Original Message----- > > > From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] > > > Sent: Thursday, May 14, 2015 4:44 AM > > > To: histonet at lists.utsouthwestern.edu > > > Subject: [Histonet] OJT Histotechs/Training > > > > > > Hello Histonet, > > > > > > I'm curious how people are dealing with on-the-job-trained > histotechs. Many people are seeing a shortage in techs, and in my opinion > OJT will become more common than it already is. Does anyone have an > 'official' training program? Requirements to pass the exam? > Qualifications to be able to be trained on-the-job? I'd like to consider > having some kind of plan in place when I don't have an HT/HTL applicant but > have folks who, if they get the experience, are otherwise qualified to sit > for the exam. If anyone has a similar situation or experience to share I > would appreciate it! > > > > > > Thanks, > > > Mike > > > > > > Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | > > > Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | > > > mjdessoye at commonwealthhealth.net > > et> | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 > > > | Fax: 570-552-1486 > > > > > > > > > > > > ---------------------------------------------------------------------- > > > ---- > > > Disclaimer: This electronic message may contain information that is > Proprietary, Confidential, or legally privileged or protected. It is > intended only for the use of the individual(s) and entity named in the > message. If you are not an intended recipient of this message, please > notify the sender immediately and delete the material from your computer. > Do not deliver, distribute or copy this message and do not disclose its > contents or take any action in reliance on the information it contains. > > > _______________________________________________ > > > Histonet mailing list > > > Histonet at lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet at lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > > The information contained in this message may be privileged and > confidential > > and protected from disclosure. If the reader of this message is not the > > intended recipient, or an employee or agent responsible for delivering > this > > message to the intended recipient, you are hereby notified that any > > dissemination, distribution or copying of this communication is strictly > > prohibited. If you have received this communication in error, please > notify > > us immediately by replying to the message and deleting it from your > computer. > > Thank you. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joelleweaver at hotmail.com Thu May 14 15:33:57 2015 From: joelleweaver at hotmail.com (Joelle Weaver) Date: Thu, 14 May 2015 20:33:57 +0000 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: References: Message-ID: Degree/college credit requirements since 2005 for OJT. If they have a degree, you would need to provide/document training under a direction of board certified pathologist. Programs often have a theory ( class room) and practical component that is completed on site at affiliate laboratories. Recommend becoming an affiliate site for program and accepting clinical students who are registry eligible when they complete both portions of the program. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: mjdessoye at commonwealthhealth.net > To: histonet at lists.utsouthwestern.edu > Date: Thu, 14 May 2015 11:43:53 +0000 > Subject: [Histonet] OJT Histotechs/Training > > Hello Histonet, > > I'm curious how people are dealing with on-the-job-trained histotechs. Many people are seeing a shortage in techs, and in my opinion OJT will become more common than it already is. Does anyone have an 'official' training program? Requirements to pass the exam? Qualifications to be able to be trained on-the-job? I'd like to consider having some kind of plan in place when I don't have an HT/HTL applicant but have folks who, if they get the experience, are otherwise qualified to sit for the exam. If anyone has a similar situation or experience to share I would appreciate it! > > Thanks, > Mike > > Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 > > > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gentras at auburn.edu Thu May 14 15:43:53 2015 From: gentras at auburn.edu (Atoska Gentry) Date: Thu, 14 May 2015 20:43:53 +0000 Subject: [Histonet] microscope slides Message-ID: <7CED05D5DD63924196370F81D21F2E92B4496171@exmb1> Good afternoon, does cleaning dust from adhesion superfrost plus microscope slides interfere with paraffin section adhesion? If not will someone be so kind as to share their tried and proven method ASAP? Thanks in advance. ~ Atoska From TNMayer at mdanderson.org Thu May 14 15:46:42 2015 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Thu, 14 May 2015 20:46:42 +0000 Subject: [Histonet] OJT Histotechs/Training Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC882247AACE@D1PWPEXMBX05.mdanderson.edu> One good way to find techs is to offer to become a clinical affiliate for a program. Most programs struggle with attracting students and providing them with clinical affiliates to fine tune their skills. It may not matter that the school is not located near you, the student may have family nearby to stay with. We are always looking for long distance affiliates, that way we can attract an out-of-state student and not saturate the local area. I have students who want to relocate to different areas and just for a change and this helps them do so. We also get calls from applicants who don't mind moving to us for 9-10 months, as long as they can go home when they finish. If the program is agreeable to this, the specifics can be worked out, such as what skills are entry level and the length of the time the student is at your facility. Ours is called an Internship and the student is at the facility for 12 weeks. They come in knowing basic embedding, cutting, routine staining, specials, and have performed a minimum of three IHC stains. Two are manual and one automated. Some programs keep the students in house for some time before they leave for internship, while others leave the technical training to the clinics. It all depends on what is available. This would be a low cost way to see if you like a person, can train them and are willing to teach. Some students are looking to relocate just before graduation, so a move for an internship is a consideration. Many times it is the expectations of the trainer that are not aligned with the skill level of entry-level techs and that can cause problems. This way the person can come in with an assessment of the skill level and the OJT phase can begin. If the affiliate chooses to hire the student, great. If not, then no harm. At least you get to say that you tried and did not have to waste money doing so. It is not a source of free labor, but a way of accurately assessing a person's fit for your needs. Many allied health programs (not just histo) are doing this and it helps to showcase different labs and programs. Just my two cents. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 1 Date: Thu, 14 May 2015 17:07:06 +0000 From: "Morken, Timothy" To: Pam Marcum , Lisa Roy Cc: Histonet , Michael Dessoye Subject: Re: [Histonet] OJT Histotechs/Training Message-ID: <761E2B5697F795489C8710BCC72141FF36831E99 at ex07.net.ucsf.edu> Content-Type: text/plain; charset="utf-8" I think there is some actor from the CSI series that has done some of this work promoting lab techs... Tim Morken -----Original Message----- From: Pam Marcum [mailto:mucram11 at comcast.net] Sent: Thursday, May 14, 2015 9:18 AM To: Lisa Roy Cc: Histonet; Michael Dessoye Subject: Re: [Histonet] OJT Histotechs/Training I understand and agree with everything being said and feel we do need more education in getting your registry, as Histology is changing and growing.??We need to be prepared to grow with it, much as we did when IHC first came into Histology and many thought it would go to the MTs.?? ? The one thing that has not changed in the 50 years I have done Histology is the fact that no one outside of AP knows what a Histologist is or what we do.? (I'm tried of being asked "Oh what kind of history is that?")? Until we change that and get more information about the field and advantages we will still be in the straights we are in now.? No one joining because so few people even know what we do or that there is an opportunity here.? If you don't know what Histology is why would you even look at the field.? I know about and have done school visits, career days etc; and those are not enough.? ? We need a spokesperson or celebrity?who has needed our services and not even known we, Histology, were the ones who did the slides their wonderful doctors used to save their lives.? This person or persons needs to speak loud and strong the way Robin Roberts has done on TV for her doctors and?help.?However; Histology was neven mentioned in those gratis moments.?I have only known one?person in NSH who suggested this and no one listened.? If?they can't see you or know you - you don't exist.??Can we all take off the blinders and?look at what we need in publicity and stop waiting for NSH and ASCP to do it.???Then we can offer these possible future HTs and HTLs something, like being recognized as full laboratory professionals and a higher level of lab aide. ? Just my thoughts (for many years and spoken often) ? Pam Marcum ----- Original Message ----- From: "Lisa Roy" To: "Michael Dessoye" , "Histonet" Sent: Thursday, May 14, 2015 7:55:19 AM Subject: Re: [Histonet] OJT Histotechs/Training I currently have 3 open tech positions and don't have any qualified applicants applying for the job. ?I have recently taken a lab aide that showed interest and aptitude and began OJT. ?With less than 30 schools in the country actually teaching histology, this is one day going to be the way. ?Already having a bachelors in biology, my aide qualifies to sit for the ASCP exam once he has completed one full year of tech work and has a pathologist willing to review his work and sign off on the ASCP paperwork. ?Without going through a traditional program, one must have an associates or bachelor's degree with a ?certain amount of Chemistry and Science credits. ?As far as the training, I started with embedding and moved on from there to cutting and then special staining. ?All along way, working on troubleshooting and documenting EVERYTHING. ?Some places will hire someone with only a high school diploma as long as they have previous HT experience. ?I think the specifics of what each institution would deem a qualified trainee will vary from place to place. ?Smaller hospitals or labs may be okay training someone with aptitude that doesn't necessarily fit the ASCP exam qualifications, but large corporations might really insist that the trainee be certifiable at some point. Frankly, I think taking someone that shows an interest and has the knowledge to be a great tech is better than hiring someone that you may not know what you are getting. ?Doing OJT ensures that you are teaching the candidate exactly how you want things done and not having to accept the bad habits of someone that has been doing it a long time and set in their own ways. Good luck Lisa ? ? -----Original Message----- From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] Sent: Thursday, May 14, 2015 7:44 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] OJT Histotechs/Training Hello Histonet, I'm curious how people are dealing with on-the-job-trained histotechs. ?Many people are seeing a shortage in techs, and in my opinion OJT will become more common than it already is. ?Does anyone have an 'official' training program? ?Requirements to pass the exam? ?Qualifications to be able to be trained on-the-job? ?I'd like to consider having some kind of plan in place when I don't have an HT/HTL applicant but have folks who, if they get the experience, are otherwise qualified to sit for the exam. ?If anyone has a similar situation or experience to share I would appreciate it! Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 From mucram11 at comcast.net Thu May 14 15:53:13 2015 From: mucram11 at comcast.net (=?utf-8?B?bXVjcmFtMTE=?=) Date: Thu, 14 May 2015 20:53:13 GMT Subject: [Histonet] OJT Histotechs/Training Message-ID: <000f4242.440fbebc55d0f1ef@comcast.net> Hazel and I are both supervisors Little Rock. She has one opening I have two and I know of three other labs looking right now. We have a histlogy school here and they had three students so you can see the issue. I hired one and the other two were not looking in LR for jobs. We have a med tech school and they don't rotate through histology and in talking to a student from the program the other day she had no idea there was a shortage or that she could qualify to become one. So let's all realize this more than a local issue. Larger cities have more options and still have the same problem. I am attempting to get a rotation and it is not going to be easy. ? Sent from my Verizon 4G LTE Smartphone ------ Original message------From: Heather MarlattDate: Thu, May 14, 2015 3:31 PMTo: joelle weaver;Cc: histonet at lists.utsouthwestern.edu;Horn, Hazel V;Subject:Re: [Histonet] OJT Histotechs/Training I agree and would also like to "like" Pams response.On May 14, 2015 1:26 PM, "Joelle Weaver" wrote:> I know it is hard Hazel. I have a hard time finding well qualified people> myself. OJT only works now if you already have a degree. If we can't raise> the pay and the recognition we will have some difficulty recruiting those> people too. I know the situation well, as I used to be the sole educator> person in an HT program. It closed due to lack of enrollment and funding. I> have trained people the best way that I knew how both formally and> informally. I have driven in my car to multiple states,written articles,> gone to conferences, schools, colleges and trade shows, and did my> personal best to present topics and "sell' our profession. Admittedly,> sometimes it feels like it doesn't matter or change anything. But we have> to keep trying and pushing for our group, its best interests, its public> recognition, its compensation. There will be no budging from anyone else> I'm afraid. Change often moves like a glacier, but it does move!>>> Joelle Weaver MAOM, HTL (ASCP) QIHC>>>>>> > From: HornHV at archildrens.org> > To: joelleweaver at hotmail.com; timothy.morken at ucsf.edu;> histonet at lists.utsouthwestern.edu> > Date: Thu, 14 May 2015 14:46:40 -0500> > Subject: RE: [Histonet] OJT Histotechs/Training> >> > Joelle I agree with you. But the problem is, no one knows we exist.> OJT is the only route for some/if not most positions to be filled. We> would all love to have a choice of educated ASCP registered techs to choose> from. I have an open position and no applicants.> >> > Hazel Horn, HTL/HT (ASCP)> > Supervisor of Histology/Autopsy/Transcription> > Anatomic Pathology> > Arkansas Children's Hospital> > 1 Children's Way | Slot 820| Little Rock, AR 72202> > 501.364.4240 direct | 501.364.1241 fax> > hornhv at archildrens.org> > archildrens.org> >> >> >> >> >> > -----Original Message-----> > From: Joelle Weaver [mailto:joelleweaver at hotmail.com]> > Sent: Thursday, May 14, 2015 2:35 PM> > To: Morken, Timothy; histonet at lists.utsouthwestern.edu> > Subject: Re: [Histonet] OJT Histotechs/Training> >> > We have discussed this on the histonet many times...> >> > Most professions, and most if not all healthcare professions, require> degrees and/or certification for entry. This is how the public, other> medical professions ,and even HR-who do not know the technical- assess for> our capacity to provide care and judge the skill level needed of the> profession as they are "looking in". We all know that this isn't always> perhaps the best method to assess or measure some aspects of this> profession, but this is what they work from. There are good and bad> examples of both OJT and educated, formally trained histology> professionals. However, "education" is more than learning facts, it helps> develop many other facets of the person that are viewed as valuable to> organizations. That is why it is used as a screening tool. Please try to> value the broader perspective. Technical proficiency itself is probably> not going to be enough as the future unfolds. Though it may seem unfair if> you have worked for a very long time and learned a great deal through> experience, the bottom line is that for some employers, some environments> and outside groups- education, credentials and professionalism are the> primary criteria they use to evaluate, and they pay and recognize> accordingly.> >> > Joelle Weaver MAOM, HTL (ASCP) QIHC> >> >> >> >> >> > > From: Timothy.Morken at ucsf.edu> > > To: histonet at lists.utsouthwestern.edu> > > Date: Thu, 14 May 2015 17:28:15 +0000> > > Subject: Re: [Histonet] OJT Histotechs/Training> > >> > > Mike, yes, the vast majority of histotechs have been, are, and will> be OJT (me included). The people who take on training these people have a> responsibility to do the best they can. Most techs end up learning whatever> their lab does and so have limited knowledge. I studied a full year for the> HT and passed fine, and later the HTL. In our small lab at the time we had> a broad array of testing in histology (specials, muscle histochem,> immunochem, electron microscopy), but I found out my true lack of knowledge> when I went to Saudi Arabia and worked with techs from other countries> where they had comprehensive bachelors-level programs required for ALL lab> techs. Those from the US, all certificated, where vastly under-educated> compared to techs from other countries. It was a bit embarrassing!> > >> > > Luckily we have online courses and degrees available now - not> available in the 1980's when I started. That is a tremendous advantage to> those who are willing to take advantage of it. Other than that it will be> up to the lab management to be sure the OJT tech gets the basic instruction> according to the requirements of the ASCP exam. That is the bare bones> knowledge necessary to function. Even then the experience in the lab is key> to whether the knowledge is just regurgitated or practiced. Lab management> has a responsibility to be sure good lab practices are ingrained during> training. It is a big job.> > >> > > As an aside, there are some people out there trying to break into> histology but do not work in a histo lab, or work in a lab that does not> support their desire to get certificated (which is practically criminal in> my view). I talked to a person recently who is working in a histo lab but> is trying to find a lab to do special stains they do not do in the lab they> are working in. Their lab will not buy them the reagents necessary and> actually told this person that they will not help them get certificated> because they feel the person will move on to get better pay elsewhere.> > >> > > I agree with another thought expressed that finding a person excited> about getting into histology can lead to a good tech. I had a person just> show up cold one day saying he really wanted to work in the histo lab - he> had learned some histology in a research lab and did not realize it could> be a full time profession until he stumbled on our lab one day. He had a> good background but we had no histo jobs open, but we happened to have a> new grossing lab aid job opening and he managed to get that job. The> expectation is that he will eventually work his way into histology. He's> happy to have his foot in the door, and we are happy to have an> enthusiastic person with a plan for advancement.> > >> > > Tim Morken> > > Pathology Site Manager, Parnassus> > > Supervisor, Electron Microscopy/Neuromuscular Special Studies> > > Department of Pathology UC San Francisco Medical Center> > >> > >> > > -----Original Message-----> > > From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net]> > > Sent: Thursday, May 14, 2015 4:44 AM> > > To: histonet at lists.utsouthwestern.edu> > > Subject: [Histonet] OJT Histotechs/Training> > >> > > Hello Histonet,> > >> > > I'm curious how people are dealing with on-the-job-trained> histotechs. Many people are seeing a shortage in techs, and in my opinion> OJT will become more common than it already is. Does anyone have an> 'official' training program? Requirements to pass the exam?> Qualifications to be able to be trained on-the-job? I'd like to consider> having some kind of plan in place when I don't have an HT/HTL applicant but> have folks who, if they get the experience, are otherwise qualified to sit> for the exam. If anyone has a similar situation or experience to share I> would appreciate it!> > >> > > Thanks,> > > Mike> > >> > > Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor |> > > Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health |> > > mjdessoye at commonwealthhealth.net > > et> | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432> > > | Fax: 570-552-1486> > >> > >> > >> > > ----------------------------------------------------------------------> > > ----> > > Disclaimer: This electronic message may contain information that is> Proprietary, Confidential, or legally privileged or protected. It is> intended only for the use of the individual(s) and entity named in the> message. If you are not an intended recipient of this message, please> notify the sender immediately and delete the material from your computer.> Do not deliver, distribute or copy this message and do not disclose its> contents or take any action in reliance on the information it contains.> > > _______________________________________________> > > Histonet mailing list> > > Histonet at lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > >> > > _______________________________________________> > > Histonet mailing list> > > Histonet at lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> >> > _______________________________________________> > Histonet mailing list> > Histonet at lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> >> >> ******************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************> > The information contained in this message may be privileged and> confidential> > and protected from disclosure. If the reader of this message is not the> > intended recipient, or an employee or agent responsible for delivering> this> > message to the intended recipient, you are hereby notified that any> > dissemination, distribution or copying of this communication is strictly> > prohibited. If you have received this communication in error, please> notify> > us immediately by replying to the message and deleting it from your> computer.> > Thank you.>> _______________________________________________> Histonet mailing list> Histonet at lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet>_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From ihcworkshop at gmail.com Thu May 14 17:16:39 2015 From: ihcworkshop at gmail.com (IHC Workshop) Date: Thu, 14 May 2015 15:16:39 -0700 Subject: [Histonet] Hand-on wet IHC Workshop for Researchers Message-ID: Hello Histonet: IHC. Hands-on Wet Lab course extensively covers staining of all animal tissues, mouse-on-mouse; rabbit-on-rabbit, Lots of troubleshooting in "IHC workshop for Researchers", June 25th & 26th in San Francisco Bay Area. Approved by NSH for 12 CEU. A few spots still left. Reply to this email for more info. From BDeBrosse-Serra at isisph.com Thu May 14 17:48:19 2015 From: BDeBrosse-Serra at isisph.com (Bea DeBrosse-Serra) Date: Thu, 14 May 2015 22:48:19 +0000 Subject: [Histonet] Hand-on wet IHC Workshop for Researchers In-Reply-To: References: Message-ID: Could you please send some more information on that and where to register? Thanks, Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: IHC Workshop [mailto:ihcworkshop at gmail.com] Sent: Thursday, May 14, 2015 3:17 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Hand-on wet IHC Workshop for Researchers Hello Histonet: IHC. Hands-on Wet Lab course extensively covers staining of all animal tissues, mouse-on-mouse; rabbit-on-rabbit, Lots of troubleshooting in "IHC workshop for Researchers", June 25th & 26th in San Francisco Bay Area. Approved by NSH for 12 CEU. A few spots still left. Reply to this email for more info. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aeck at dh.org Fri May 15 07:04:41 2015 From: aeck at dh.org (Eck, Allison) Date: Fri, 15 May 2015 12:04:41 +0000 Subject: [Histonet] adjunct faculty position Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A97188F1E@DH-MAIL01.dhorg.org> Harcum College in Bryn Mawr, PA is currently looking for an adjunct faculty member for its histotechnology program. Adjunct Faculty: Online Histology Instructor Harcum College's Lab Science Department is looking for an adjunct online histology (HT) instructor. Duties and Responsibilities: instruction using Webstudy Learning Management System (LMS). The instructor is expected to actively engage students via LMS email and discussion forums, grade assignments and exams in a timely manner, modify and upload learning material as needed into the LMS. Submission of weekly attendance and final grades. Syllabi updates each semester Attendance at annual advisory board/department meetings and other meetings as requested by the program director Mandatory annual participation in continuing education activities Regular and ongoing communication with HT program director and clinical coordinator Annual completion of course assessment information Qualifications: ASCP certified as an HT or HTL with a minimum of 5 years of clinical experience in a full service histology lab Knowledge and ability to teach the following HT material in an online platform: Fixation, Processing, Embedding/Microtomy, Staining, and Laboratory Operations. teaching experience in a college setting preferred-specifically online teaching using a LMS From tejohnson at genoptix.com Fri May 15 11:07:32 2015 From: tejohnson at genoptix.com (Teri Johnson) Date: Fri, 15 May 2015 16:07:32 +0000 Subject: [Histonet] Job openings and no candidates Message-ID: Happy Friday! Ideally when there is a job opening we can attract a well-trained certified HT or HTL and life is good. But that doesn't happen often, and actually was the exact reason I got a chance to train OTJ back in the early 80s. So, very little has changed indeed. In response to the recent emails lamenting the lack of qualified candidates for current open positions, I think we need to be a bit more creative in how we recruit people. We already know that nobody knows we exist, and even though there some schools in the US specifically devoted to histology training, we don't seem to be making headway. There is a group of individuals already out there that we may be able to tap into. Both already have associate degrees and technical laboratory background. Veterinary Medical Technicans and Medical Laboratory Technicians may be ripe for the plucking. Career advancement and professional development would be a great carrot for them I think without the need to get a bachelor's degree unless they wanted to. The background is at least a little more in line with the overseas Laboratory Scientist positions, and anyone who is a VMT, MLT, or MT and also a histology professional can tell you the extra knowledge comes in handy in real life. So, why not also find schools that have these programs and throw job openings up on their bulletin board? We may not get many nibbles, but some is better than none. Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From WesterM at MedImmune.com Fri May 15 11:08:06 2015 From: WesterM at MedImmune.com (Wester, Martha) Date: Fri, 15 May 2015 16:08:06 +0000 Subject: [Histonet] cut slide storage under Nitrogen gas Message-ID: Hi HistoNet- I'd love to get any and all feedback about storage conditions for cut slides and methods use to minimize epitope oxidation. And more specifically, if anyone is using nitrogen gas cabinets to do so. Another part of the equation we are trying to look at is defining time periods of storage, possibly ranging from weeks to maybe 1-2 years. Thanks for any experiences you'd care to share! Martha Wester Manager R&D MedImmune One MedImmune Way Gaithersburg, MD 20878 To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation. From jkrupp at deltacollege.edu Fri May 15 11:31:56 2015 From: jkrupp at deltacollege.edu (Jon Krupp) Date: Fri, 15 May 2015 09:31:56 -0700 Subject: [Histonet] Job openings and no candidates In-Reply-To: References: Message-ID: <35B5A647-58F4-4857-97CA-D95F3C12C965@deltacollege.edu> I have been following this and would like to ask a few questions. I am part of a community college electron microscope training program. We teach the fundamentals of both biological and materials EM, both SEM and TEM. Our biological students learn plastic sectioning for LM and EM. They do not learn much paraffin technique, but the ones I have shown have caught on quickly. They have all been exposed to fixation, dehydration, and embedding in plastic. They all know how to work safely in a lab. Most finish with an associates degree in general science, so they all have basic chemistry, biology, etc. They do not have anything like a real histology course. While our materials students seem to find jobs, especially in nearby computer firms like Intel, IBM, Western Digital etc., our bio students have a harder time. Some have asked about histology, I show them the ASCP rules and they get discouraged, thinking they will never find a place to get in that year of OJT. There are few NACCLS programs in our area, and some of the course work in such a program would be redundant for our students. What is the likelihood that some of my students would qualify for OJT in a histology lab, and how can I alert them to the possibilities? Jon Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkrupp at deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College From mucram11 at comcast.net Fri May 15 11:56:05 2015 From: mucram11 at comcast.net (Pam Marcum) Date: Fri, 15 May 2015 16:56:05 +0000 (UTC) Subject: [Histonet] Job openings and no candidates In-Reply-To: <35B5A647-58F4-4857-97CA-D95F3C12C965@deltacollege.edu> References: <35B5A647-58F4-4857-97CA-D95F3C12C965@deltacollege.edu> Message-ID: <2064423005.3586076.1431708965920.JavaMail.zimbra@comcast.net> If I understand this correctly your students would need to find a hospital with a pathologist willing to allow them to work for one year as basically a lab aide while they are getting the Histology training they need to qualify and sit for the registry.??? Honestly, I wish your students were closer to us as I learned a great deal from TEM and had to do it alone.? It has helped me in many areas of Histology over the years.? The 2 year degree allows you to sit for the registry after being a trainee in Histology for one year as stated above.? While the basic reagents are different there is enough crossover with EM and Histology to give the person a great balance for doing both areas.? You might want to keep a couple of basic Histology texts around for them to see and look over for tips.? ? Maybe you could even get a local hospital to allow your students to tour the lab.? Little Rock has one Histology School with limited enrollment and are part of the enrichment program.? We have each of the students in our lab for two weeks to see what we do and how we are different from other labs they work in.? It is really a two week job interview in a way.? We get to see them in action and judge their interest and participation in the work place.? Tell them not be discouraged please and it might be an option for your school to contact hospitals or have them do it for possible training.? They just need to know how to present what they have learned and integrate it with Histology.? ? Pam Marcum UAMS Little Rock AR ? ----- Original Message ----- From: "Jon Krupp" To: "Histonet" Sent: Friday, May 15, 2015 11:31:56 AM Subject: Re: [Histonet] Job openings and no candidates I have been following this and would like to ask a few questions. I am part of a community college electron microscope training program. We teach the fundamentals of both biological and materials EM, both SEM and TEM. Our biological students learn plastic sectioning for LM and EM. They do not learn much paraffin technique, but the ones I have shown have caught on quickly. They have all been exposed to fixation, dehydration, and embedding in plastic. They all know how to work safely in a lab. Most finish with an associates degree in general science, so they all have basic chemistry, biology, etc. They do not have anything like a real histology course. While our materials students seem to find jobs, especially in nearby computer firms like Intel, IBM, Western Digital etc., our bio students have a harder time. Some have asked about histology, I show them the ASCP rules and they get discouraged, thinking they will never find a place to get in that year of OJT. There are few NACCLS programs in our area, and some of the course work in such a program would be redundant for our students. What is the likelihood that some of my students would qualify for OJT in a histology lab, and how can I alert them to the possibilities? Jon Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA ?95207 209-954-5284 jkrupp at deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.tolentino82 at gmail.com Fri May 15 12:59:08 2015 From: a.tolentino82 at gmail.com (Aimee Tolentino) Date: Fri, 15 May 2015 10:59:08 -0700 Subject: [Histonet] Immigrating to Canada Message-ID: Hello! My name is Aimee and I currently have my HT(ASCP) and have been working in the field of histology for 9 years now. I'm thinking about moving to Canada and would like to know how I can get certified by their government. I also have experience in IHC and general lab management. Any help and/or information would be greatly appreciated. Thank you! Aimee From anna.coffey at nih.gov Fri May 15 13:25:44 2015 From: anna.coffey at nih.gov (Coffey, Anna (NIH/NCI) [C]) Date: Fri, 15 May 2015 18:25:44 +0000 Subject: [Histonet] Job openings and no candidates Message-ID: <5C3E10119A1B824FBE92B08279F74A9101799D27@msgb10.nih.gov> Hi Jon, I just wanted to mention the possibility of your students finding jobs in research labs. Many research labs at universities utilize techs in histology that are not certified and, as long as they're in the US or Canada, that experience does meet ASCP requirements to sit for the exam. I've worked in research labs for the last 3 years and took my HTL exam last spring. This is great because the techs can still be paid as full-time employees (though less than a certified tech) with benefits while working towards their certification. I'd be happy to talk more with you about this if you have questions or think it might be helpful! Best, Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey at nih.gov 301-846-1730 ----- Original Message ----- From: "Jon Krupp" To: "Histonet" Sent: Friday, May 15, 2015 11:31:56 AM Subject: Re: [Histonet] Job openings and no candidates I have been following this and would like to ask a few questions. I am part of a community college electron microscope training program. We teach the fundamentals of both biological and materials EM, both SEM and TEM. Our biological students learn plastic sectioning for LM and EM. They do not learn much paraffin technique, but the ones I have shown have caught on quickly. They have all been exposed to fixation, dehydration, and embedding in plastic. They all know how to work safely in a lab. Most finish with an associates degree in general science, so they all have basic chemistry, biology, etc. They do not have anything like a real histology course. While our materials students seem to find jobs, especially in nearby computer firms like Intel, IBM, Western Digital etc., our bio students have a harder time. Some have asked about histology, I show them the ASCP rules and they get discouraged, thinking they will never find a place to get in that year of OJT. There are few NACCLS programs in our area, and some of the course work in such a program would be redundant for our students. What is the likelihood that some of my students would qualify for OJT in a histology lab, and how can I alert them to the possibilities? Jon Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA ?95207 209-954-5284 jkrupp at deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 138, Issue 19 ***************************************** From areichard at lmhealth.org Fri May 15 13:39:34 2015 From: areichard at lmhealth.org (Amanda Reichard) Date: Fri, 15 May 2015 14:39:34 -0400 Subject: [Histonet] Job openings and no candidates In-Reply-To: <5C3E10119A1B824FBE92B08279F74A9101799D27@msgb10.nih.gov> References: <5C3E10119A1B824FBE92B08279F74A9101799D27@msgb10.nih.gov> Message-ID: Jon, I also went the research route. I have a Bachelors, and was able to sit for the exam after a year working as a non-certified histology technician. It was great that I was able to work full time with benefits and have that count as my experience for exam qualifications. Amanda Reichard, HTL (ASCP)cm Histotechnologist Laboratory Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (740) 348-4157 ________________________________________ From: Coffey, Anna (NIH/NCI) [C] [anna.coffey at nih.gov] Sent: Friday, May 15, 2015 2:25 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Job openings and no candidates Hi Jon, I just wanted to mention the possibility of your students finding jobs in research labs. Many research labs at universities utilize techs in histology that are not certified and, as long as they're in the US or Canada, that experience does meet ASCP requirements to sit for the exam. I've worked in research labs for the last 3 years and took my HTL exam last spring. This is great because the techs can still be paid as full-time employees (though less than a certified tech) with benefits while working towards their certification. I'd be happy to talk more with you about this if you have questions or think it might be helpful! Best, Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey at nih.gov 301-846-1730 ----- Original Message ----- From: "Jon Krupp" To: "Histonet" Sent: Friday, May 15, 2015 11:31:56 AM Subject: Re: [Histonet] Job openings and no candidates I have been following this and would like to ask a few questions. I am part of a community college electron microscope training program. We teach the fundamentals of both biological and materials EM, both SEM and TEM. Our biological students learn plastic sectioning for LM and EM. They do not learn much paraffin technique, but the ones I have shown have caught on quickly. They have all been exposed to fixation, dehydration, and embedding in plastic. They all know how to work safely in a lab. Most finish with an associates degree in general science, so they all have basic chemistry, biology, etc. They do not have anything like a real histology course. While our materials students seem to find jobs, especially in nearby computer firms like Intel, IBM, Western Digital etc., our bio students have a harder time. Some have asked about histology, I show them the ASCP rules and they get discouraged, thinking they will never find a place to get in that year of OJT. There are few NACCLS programs in our area, and some of the course work in such a program would be redundant for our students. What is the likelihood that some of my students would qualify for OJT in a histology lab, and how can I alert them to the possibilities? Jon Jonathan Krupp Applied Science, Business & Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA ?95207 209-954-5284 jkrupp at deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 138, Issue 19 ***************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From abadesuyi at nrh-ok.com Fri May 15 14:44:27 2015 From: abadesuyi at nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Fri, 15 May 2015 14:44:27 -0500 Subject: [Histonet] Tissue-Tek VIP 6 Manual Message-ID: <04EE4F75BB5FB246ADB68D69B7460443A37D487FBC@MAIL.nrhnt.nrh-ok.com> Hi everyone, I hope you guys are doing great. Please I am in need of Tissue-Tek VIP 6 Manual in (hard-copy or PDF) and will greatly appreciate it, if you guys can help me. Best regards, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, 901 N. Porter Avenue Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From JMacDonald at mtsac.edu Sat May 16 22:02:34 2015 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Sat, 16 May 2015 20:02:34 -0700 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC882247AACE@D1PWPEXMBX05.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC882247AACE@D1PWPEXMBX05.mdanderson.edu> Message-ID: This is an issue with our program as well. We have a difficult time finding clinical sites for our students. Many people want to hire trained individuals, but don't want to invest any time in the training. Our students receive a great deal of hands-on time in the student laboratory, but need "real life" experience. Jennifer MacDonald Mt. San Antonio College From: "Mayer,Toysha N" To: "'histonet at lists.utsouthwestern.edu'" Date: 05/14/2015 01:48 PM Subject: Re: [Histonet] OJT Histotechs/Training One good way to find techs is to offer to become a clinical affiliate for a program. Most programs struggle with attracting students and providing them with clinical affiliates to fine tune their skills. It may not matter that the school is not located near you, the student may have family nearby to stay with. We are always looking for long distance affiliates, that way we can attract an out-of-state student and not saturate the local area. I have students who want to relocate to different areas and just for a change and this helps them do so. We also get calls from applicants who don't mind moving to us for 9-10 months, as long as they can go home when they finish. If the program is agreeable to this, the specifics can be worked out, such as what skills are entry level and the length of the time the student is at your facility. Ours is called an Internship and the student is at the facility for 12 weeks. They come in knowing basic embedding, cutting, routine staining, specials, and have performed a minimum of three IHC stains. Two are manual and one automated. Some programs keep the students in house for some time before they leave for internship, while others leave the technical training to the clinics. It all depends on what is available. This would be a low cost way to see if you like a person, can train them and are willing to teach. Some students are looking to relocate just before graduation, so a move for an internship is a consideration. Many times it is the expectations of the trainer that are not aligned with the skill level of entry-level techs and that can cause problems. This way the person can come in with an assessment of the skill level and the OJT phase can begin. If the affiliate chooses to hire the student, great. If not, then no harm. At least you get to say that you tried and did not have to waste money doing so. It is not a source of free labor, but a way of accurately assessing a person's fit for your needs. Many allied health programs (not just histo) are doing this and it helps to showcase different labs and programs. Just my two cents. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 1 Date: Thu, 14 May 2015 17:07:06 +0000 From: "Morken, Timothy" To: Pam Marcum , Lisa Roy Cc: Histonet , Michael Dessoye Subject: Re: [Histonet] OJT Histotechs/Training Message-ID: <761E2B5697F795489C8710BCC72141FF36831E99 at ex07.net.ucsf.edu> Content-Type: text/plain; charset="utf-8" I think there is some actor from the CSI series that has done some of this work promoting lab techs... Tim Morken -----Original Message----- From: Pam Marcum [mailto:mucram11 at comcast.net] Sent: Thursday, May 14, 2015 9:18 AM To: Lisa Roy Cc: Histonet; Michael Dessoye Subject: Re: [Histonet] OJT Histotechs/Training I understand and agree with everything being said and feel we do need more education in getting your registry, as Histology is changing and growing.??We need to be prepared to grow with it, much as we did when IHC first came into Histology and many thought it would go to the MTs.?? ? The one thing that has not changed in the 50 years I have done Histology is the fact that no one outside of AP knows what a Histologist is or what we do.? (I'm tried of being asked "Oh what kind of history is that?")? Until we change that and get more information about the field and advantages we will still be in the straights we are in now.? No one joining because so few people even know what we do or that there is an opportunity here.? If you don't know what Histology is why would you even look at the field.? I know about and have done school visits, career days etc; and those are not enough.? ? We need a spokesperson or celebrity?who has needed our services and not even known we, Histology, were the ones who did the slides their wonderful doctors used to save their lives.? This person or persons needs to speak loud and strong the way Robin Roberts has done on TV for her doctors and?help.?However; Histology was neven mentioned in those gratis moments.?I have only known one?person in NSH who suggested this and no one listened.? If?they can't see you or know you - you don't exist.??Can we all take off the blinders and?look at what we need in publicity and stop waiting for NSH and ASCP to do it.???Then we can offer these possible future HTs and HTLs something, like being recognized as full laboratory professionals and a higher level of lab aide. ? Just my thoughts (for many years and spoken often) ? Pam Marcum ----- Original Message ----- From: "Lisa Roy" To: "Michael Dessoye" , "Histonet" Sent: Thursday, May 14, 2015 7:55:19 AM Subject: Re: [Histonet] OJT Histotechs/Training I currently have 3 open tech positions and don't have any qualified applicants applying for the job. ?I have recently taken a lab aide that showed interest and aptitude and began OJT. ?With less than 30 schools in the country actually teaching histology, this is one day going to be the way. ?Already having a bachelors in biology, my aide qualifies to sit for the ASCP exam once he has completed one full year of tech work and has a pathologist willing to review his work and sign off on the ASCP paperwork. ?Without going through a traditional program, one must have an associates or bachelor's degree with a ?certain amount of Chemistry and Science credits. ?As far as the training, I started with embedding and moved on from there to cutting and then special staining. ?All along way, working on troubleshooting and documenting EVERYTHING. ?Some places will hire someone with only a high school diploma as long as they have previous HT experience. ?I think the specifics of what each in stitution would deem a qualified trainee will vary from place to place. ?Smaller hospitals or labs may be okay training someone with aptitude that doesn't necessarily fit the ASCP exam qualifications, but large corporations might really insist that the trainee be certifiable at some point. Frankly, I think taking someone that shows an interest and has the knowledge to be a great tech is better than hiring someone that you may not know what you are getting. ?Doing OJT ensures that you are teaching the candidate exactly how you want things done and not having to accept the bad habits of someone that has been doing it a long time and set in their own ways. Good luck Lisa ? ? -----Original Message----- From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] Sent: Thursday, May 14, 2015 7:44 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] OJT Histotechs/Training Hello Histonet, I'm curious how people are dealing with on-the-job-trained histotechs. ?Many people are seeing a shortage in techs, and in my opinion OJT will become more common than it already is. ?Does anyone have an 'official' training program? ?Requirements to pass the exam? ?Qualifications to be able to be trained on-the-job? ?I'd like to consider having some kind of plan in place when I don't have an HT/HTL applicant but have folks who, if they get the experience, are otherwise qualified to sit for the exam. ?If anyone has a similar situation or experience to share I would appreciate it! Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver at hotmail.com Sun May 17 05:38:28 2015 From: joelleweaver at hotmail.com (Joelle Weaver) Date: Sun, 17 May 2015 10:38:28 +0000 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: References: <47E9B2C01DDDD94881EACD2DC44EBC882247AACE@D1PWPEXMBX05.mdanderson.edu>, Message-ID: I will speak to my laboratory director about this. I know the situation first hand from my previous experience! Joelle Weaver MAOM, HTL (ASCP) QIHC > To: TNMayer at mdanderson.org > From: JMacDonald at mtsac.edu > Date: Sat, 16 May 2015 20:02:34 -0700 > Subject: Re: [Histonet] OJT Histotechs/Training > CC: histonet at lists.utsouthwestern.edu > > This is an issue with our program as well. We have a difficult time > finding clinical sites for our students. Many people want to hire trained > individuals, but don't want to invest any time in the training. Our > students receive a great deal of hands-on time in the student laboratory, > but need "real life" experience. > Jennifer MacDonald > Mt. San Antonio College > > > > From: "Mayer,Toysha N" > To: "'histonet at lists.utsouthwestern.edu'" > > Date: 05/14/2015 01:48 PM > Subject: Re: [Histonet] OJT Histotechs/Training > > > > One good way to find techs is to offer to become a clinical affiliate for > a program. Most programs struggle with attracting students and providing > them with clinical affiliates to fine tune their skills. > It may not matter that the school is not located near you, the student may > have family nearby to stay with. > We are always looking for long distance affiliates, that way we can > attract an out-of-state student and not saturate the local area. I have > students who want to relocate to different areas and just for a change and > this helps them do so. We also get calls from applicants who don't mind > moving to us for 9-10 months, as long as they can go home when they > finish. > If the program is agreeable to this, the specifics can be worked out, such > as what skills are entry level and the length of the time the student is > at your facility. > Ours is called an Internship and the student is at the facility for 12 > weeks. They come in knowing basic embedding, cutting, routine staining, > specials, and have performed a minimum of three IHC stains. Two are > manual and one automated. > Some programs keep the students in house for some time before they leave > for internship, while others leave the technical training to the clinics. > It all depends on what is available. > This would be a low cost way to see if you like a person, can train them > and are willing to teach. > Some students are looking to relocate just before graduation, so a move > for an internship is a consideration. > Many times it is the expectations of the trainer that are not aligned with > the skill level of entry-level techs and that can cause problems. This > way the person can come in with an assessment of the skill level and the > OJT phase can begin. If the affiliate chooses to hire the student, great. > If not, then no harm. At least you get to say that you tried and did not > have to waste money doing so. It is not a source of free labor, but a way > of accurately assessing a person's fit for your needs. > Many allied health programs (not just histo) are doing this and it helps > to showcase different labs and programs. > > Just my two cents. > > Sincerely, > > Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) > Instructor/Education Coordinator > Program in Histotechnology > School of Health Professions > UT M.D. Anderson Cancer Center > 713.563-3481 > > > > > > Message: 1 > Date: Thu, 14 May 2015 17:07:06 +0000 > From: "Morken, Timothy" > To: Pam Marcum , Lisa Roy > Cc: Histonet , Michael Dessoye > > Subject: Re: [Histonet] OJT Histotechs/Training > Message-ID: > <761E2B5697F795489C8710BCC72141FF36831E99 at ex07.net.ucsf.edu> > Content-Type: text/plain; charset="utf-8" > > I think there is some actor from the CSI series that has done some of this > work promoting lab techs... > > Tim Morken > > -----Original Message----- > From: Pam Marcum [mailto:mucram11 at comcast.net] > Sent: Thursday, May 14, 2015 9:18 AM > To: Lisa Roy > Cc: Histonet; Michael Dessoye > Subject: Re: [Histonet] OJT Histotechs/Training > > I understand and agree with everything being said and feel we do need more > education in getting your registry, as Histology is changing and > growing.??We need to be prepared to grow with it, much as we did when IHC > first came into Histology and many thought it would go to the MTs.?? > ? > The one thing that has not changed in the 50 years I have done Histology > is the fact that no one outside of AP knows what a Histologist is or what > we do.? (I'm tried of being asked "Oh what kind of history is that?")? > Until we change that and get more information about the field and > advantages we will still be in the straights we are in now.? No one > joining because so few people even know what we do or that there is an > opportunity here.? If you don't know what Histology is why would you even > look at the field.? I know about and have done school visits, career days > etc; and those are not enough.? > ? > We need a spokesperson or celebrity?who has needed our services and not > even known we, Histology, were the ones who did the slides their wonderful > doctors used to save their lives.? This person or persons needs to speak > loud and strong the way Robin Roberts has done on TV for her doctors > and?help.?However; Histology was neven mentioned in those gratis > moments.?I have only known one?person in NSH who suggested this and no one > listened.? If?they can't see you or know you - you don't exist.??Can we > all take off the blinders and?look at what we need in publicity and stop > waiting for NSH and ASCP to do it.???Then we can offer these possible > future HTs and HTLs something, like being recognized as full laboratory > professionals and a higher level of lab aide. > ? > Just my thoughts (for many years and spoken often) > ? > Pam Marcum > > ----- Original Message ----- > > From: "Lisa Roy" > To: "Michael Dessoye" , "Histonet" > > Sent: Thursday, May 14, 2015 7:55:19 AM > Subject: Re: [Histonet] OJT Histotechs/Training > > I currently have 3 open tech positions and don't have any qualified > applicants applying for the job. ?I have recently taken a lab aide that > showed interest and aptitude and began OJT. ?With less than 30 schools in > the country actually teaching histology, this is one day going to be the > way. ?Already having a bachelors in biology, my aide qualifies to sit for > the ASCP exam once he has completed one full year of tech work and has a > pathologist willing to review his work and sign off on the ASCP paperwork. > ?Without going through a traditional program, one must have an associates > or bachelor's degree with a ?certain amount of Chemistry and Science > credits. ?As far as the training, I started with embedding and moved on > from there to cutting and then special staining. ?All along way, working > on troubleshooting and documenting EVERYTHING. ?Some places will hire > someone with only a high school diploma as long as they have previous HT > experience. ?I think the specifics of what each in > stitution would deem a qualified trainee will vary from place to place. > ?Smaller hospitals or labs may be okay training someone with aptitude that > doesn't necessarily fit the ASCP exam qualifications, but large > corporations might really insist that the trainee be certifiable at some > point. > > Frankly, I think taking someone that shows an interest and has the > knowledge to be a great tech is better than hiring someone that you may > not know what you are getting. ?Doing OJT ensures that you are teaching > the candidate exactly how you want things done and not having to accept > the bad habits of someone that has been doing it a long time and set in > their own ways. > > Good luck > Lisa ? ? > > -----Original Message----- > From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] > Sent: Thursday, May 14, 2015 7:44 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] OJT Histotechs/Training > > Hello Histonet, > > I'm curious how people are dealing with on-the-job-trained histotechs. > ?Many people are seeing a shortage in techs, and in my opinion OJT will > become more common than it already is. ?Does anyone have an 'official' > training program? ?Requirements to pass the exam? ?Qualifications to be > able to be trained on-the-job? ?I'd like to consider having some kind of > plan in place when I don't have an HT/HTL applicant but have folks who, if > they get the experience, are otherwise qualified to sit for the exam. ?If > anyone has a similar situation or experience to share I would appreciate > it! > > Thanks, > Mike > > Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | > Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | > mjdessoye at commonwealthhealth.net > | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: > 570-552-1486 > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wbenton at cua.md Sun May 17 06:57:15 2015 From: wbenton at cua.md (Walter Benton) Date: Sun, 17 May 2015 07:57:15 -0400 Subject: [Histonet] Temporary Position Message-ID: <0B8979A204680A42B93A52B486088CD942879E402A@CUAEXH1.GCU-MD.local> Temporary Histotech needed for a Urology Pathology lab in Glen Burnie, MD. This is a 40 hour/week position and would be for approximately 4 weeks, from 6/29/15 - 7/24/15. Great working environment and highly competitive compensation. If interested, please email your resume to mhwang at cua.md. Thanks, Mike Michael Hwang Director of Recruitment Chesapeake Urology 25 Crossroads Drive, Suite 306 Owings Mills, MD 21117 Office: 443-738-2887 Fax: 443-738-2734 ChesapeakeUrology.com Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From bcequine at gmail.com Sun May 17 12:06:31 2015 From: bcequine at gmail.com (Brenda Consentino) Date: Sun, 17 May 2015 13:06:31 -0400 Subject: [Histonet] Change email address for me please to bcequine@email.com. Also, my employer has changed to Molecular Pathology Laboratory Network, Inc., Maryville, TN Message-ID: Thank you! Brenda Consentino From carl.hobbs at kcl.ac.uk Mon May 18 04:16:07 2015 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Mon, 18 May 2015 09:16:07 +0000 Subject: [Histonet] RE..... murine CD4, CD8 and CD68 for FFPE tissue Message-ID: <1431940551277.57271@kcl.ac.uk> I use the anti CD68 clone FA-11 ( obtained from Abcam). It works very well on frozen sections but, I have been unable to get any positivity on Pwax sections ( using +/- Citric acid pH6 HIER) Carl From histology81176 at att.net Mon May 18 13:28:39 2015 From: histology81176 at att.net (Histology Technician) Date: Mon, 18 May 2015 18:28:39 +0000 (UTC) Subject: [Histonet] Lot to Lot Verification Message-ID: <125661952.636415.1431973719471.JavaMail.yahoo@mail.yahoo.com> Does anyone have a Lot to Lot Verification form you'd like to share?? Trying to make one up for my lab and figure out the procedure...do you log every supply you use in the lab onto the form?? QC it and log the date/tech initial? Thanks! From Stacy_McLaughlin at cooley-dickinson.org Mon May 18 13:58:58 2015 From: Stacy_McLaughlin at cooley-dickinson.org (Stacy McLaughlin) Date: Mon, 18 May 2015 18:58:58 +0000 Subject: [Histonet] embedding center backup Message-ID: Happy Monday in Histoland, How many of you out there have a backup embedding center or paraffin dispenser for a backup? We are a smaller lab (<10k surgical cases/year). We only have one embedding center and I want to ask what others out there are doing. Thank you, Stacy Stacy McLaughlin, HT(ASCP) Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton,MA 01060 Stacy_McLaughlin at Cooley-Dickinson.org [Cooley-Dickinson.org] From lldewe at gmail.com Mon May 18 13:59:59 2015 From: lldewe at gmail.com (Loralei Dewe) Date: Mon, 18 May 2015 11:59:59 -0700 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: References: <47E9B2C01DDDD94881EACD2DC44EBC882247AACE@D1PWPEXMBX05.mdanderson.edu> Message-ID: I have a different perspective on this issue. I have been in histology for over 20 years. I worked at UC Davis in Vet. Histopath for several years. I was a histology Core facility manager and started up the facility from scratch at UC Davis Health system while running a Core Confocal microscope facility there. BUT I was in research, I wasn't in a "Pathology lab" and I don't qualify for the HT or HTL so I can't get work in the industry. Talk about a conundrum! Loralei On Sun, May 17, 2015 at 3:38 AM, Joelle Weaver wrote: > I will speak to my laboratory director about this. I know the situation > first hand from my previous experience! > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > To: TNMayer at mdanderson.org > > From: JMacDonald at mtsac.edu > > Date: Sat, 16 May 2015 20:02:34 -0700 > > Subject: Re: [Histonet] OJT Histotechs/Training > > CC: histonet at lists.utsouthwestern.edu > > > > This is an issue with our program as well. We have a difficult time > > finding clinical sites for our students. Many people want to hire > trained > > individuals, but don't want to invest any time in the training. Our > > students receive a great deal of hands-on time in the student laboratory, > > but need "real life" experience. > > Jennifer MacDonald > > Mt. San Antonio College > > > > > > > > From: "Mayer,Toysha N" > > To: "'histonet at lists.utsouthwestern.edu'" > > > > Date: 05/14/2015 01:48 PM > > Subject: Re: [Histonet] OJT Histotechs/Training > > > > > > > > One good way to find techs is to offer to become a clinical affiliate for > > a program. Most programs struggle with attracting students and providing > > them with clinical affiliates to fine tune their skills. > > It may not matter that the school is not located near you, the student > may > > have family nearby to stay with. > > We are always looking for long distance affiliates, that way we can > > attract an out-of-state student and not saturate the local area. I have > > students who want to relocate to different areas and just for a change > and > > this helps them do so. We also get calls from applicants who don't mind > > moving to us for 9-10 months, as long as they can go home when they > > finish. > > If the program is agreeable to this, the specifics can be worked out, > such > > as what skills are entry level and the length of the time the student is > > at your facility. > > Ours is called an Internship and the student is at the facility for 12 > > weeks. They come in knowing basic embedding, cutting, routine staining, > > specials, and have performed a minimum of three IHC stains. Two are > > manual and one automated. > > Some programs keep the students in house for some time before they leave > > for internship, while others leave the technical training to the clinics. > > It all depends on what is available. > > This would be a low cost way to see if you like a person, can train them > > and are willing to teach. > > Some students are looking to relocate just before graduation, so a move > > for an internship is a consideration. > > Many times it is the expectations of the trainer that are not aligned > with > > the skill level of entry-level techs and that can cause problems. This > > way the person can come in with an assessment of the skill level and the > > OJT phase can begin. If the affiliate chooses to hire the student, > great. > > If not, then no harm. At least you get to say that you tried and did > not > > have to waste money doing so. It is not a source of free labor, but a > way > > of accurately assessing a person's fit for your needs. > > Many allied health programs (not just histo) are doing this and it helps > > to showcase different labs and programs. > > > > Just my two cents. > > > > Sincerely, > > > > Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) > > Instructor/Education Coordinator > > Program in Histotechnology > > School of Health Professions > > UT M.D. Anderson Cancer Center > > 713.563-3481 > > > > > > > > > > > > Message: 1 > > Date: Thu, 14 May 2015 17:07:06 +0000 > > From: "Morken, Timothy" > > To: Pam Marcum , Lisa Roy > > Cc: Histonet , Michael Dessoye > > > > Subject: Re: [Histonet] OJT Histotechs/Training > > Message-ID: > > <761E2B5697F795489C8710BCC72141FF36831E99 at ex07.net.ucsf.edu> > > Content-Type: text/plain; charset="utf-8" > > > > I think there is some actor from the CSI series that has done some of > this > > work promoting lab techs... > > > > Tim Morken > > > > -----Original Message----- > > From: Pam Marcum [mailto:mucram11 at comcast.net] > > Sent: Thursday, May 14, 2015 9:18 AM > > To: Lisa Roy > > Cc: Histonet; Michael Dessoye > > Subject: Re: [Histonet] OJT Histotechs/Training > > > > I understand and agree with everything being said and feel we do need > more > > education in getting your registry, as Histology is changing and > > growing.??We need to be prepared to grow with it, much as we did when IHC > > first came into Histology and many thought it would go to the MTs.?? > > ? > > The one thing that has not changed in the 50 years I have done Histology > > is the fact that no one outside of AP knows what a Histologist is or what > > we do.? (I'm tried of being asked "Oh what kind of history is that?")? > > Until we change that and get more information about the field and > > advantages we will still be in the straights we are in now.? No one > > joining because so few people even know what we do or that there is an > > opportunity here.? If you don't know what Histology is why would you even > > look at the field.? I know about and have done school visits, career days > > etc; and those are not enough.? > > ? > > We need a spokesperson or celebrity?who has needed our services and not > > even known we, Histology, were the ones who did the slides their > wonderful > > doctors used to save their lives.? This person or persons needs to speak > > loud and strong the way Robin Roberts has done on TV for her doctors > > and?help.?However; Histology was neven mentioned in those gratis > > moments.?I have only known one?person in NSH who suggested this and no > one > > listened.? If?they can't see you or know you - you don't exist.??Can we > > all take off the blinders and?look at what we need in publicity and stop > > waiting for NSH and ASCP to do it.???Then we can offer these possible > > future HTs and HTLs something, like being recognized as full laboratory > > professionals and a higher level of lab aide. > > ? > > Just my thoughts (for many years and spoken often) > > ? > > Pam Marcum > > > > ----- Original Message ----- > > > > From: "Lisa Roy" > > To: "Michael Dessoye" , "Histonet" > > > > Sent: Thursday, May 14, 2015 7:55:19 AM > > Subject: Re: [Histonet] OJT Histotechs/Training > > > > I currently have 3 open tech positions and don't have any qualified > > applicants applying for the job. ?I have recently taken a lab aide that > > showed interest and aptitude and began OJT. ?With less than 30 schools in > > the country actually teaching histology, this is one day going to be the > > way. ?Already having a bachelors in biology, my aide qualifies to sit for > > the ASCP exam once he has completed one full year of tech work and has a > > pathologist willing to review his work and sign off on the ASCP > paperwork. > > ?Without going through a traditional program, one must have an associates > > or bachelor's degree with a ?certain amount of Chemistry and Science > > credits. ?As far as the training, I started with embedding and moved on > > from there to cutting and then special staining. ?All along way, working > > on troubleshooting and documenting EVERYTHING. ?Some places will hire > > someone with only a high school diploma as long as they have previous HT > > experience. ?I think the specifics of what each in > > stitution would deem a qualified trainee will vary from place to place. > > ?Smaller hospitals or labs may be okay training someone with aptitude > that > > doesn't necessarily fit the ASCP exam qualifications, but large > > corporations might really insist that the trainee be certifiable at some > > point. > > > > Frankly, I think taking someone that shows an interest and has the > > knowledge to be a great tech is better than hiring someone that you may > > not know what you are getting. ?Doing OJT ensures that you are teaching > > the candidate exactly how you want things done and not having to accept > > the bad habits of someone that has been doing it a long time and set in > > their own ways. > > > > Good luck > > Lisa ? ? > > > > -----Original Message----- > > From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] > > Sent: Thursday, May 14, 2015 7:44 AM > > To: histonet at lists.utsouthwestern.edu > > Subject: [Histonet] OJT Histotechs/Training > > > > Hello Histonet, > > > > I'm curious how people are dealing with on-the-job-trained histotechs. > > ?Many people are seeing a shortage in techs, and in my opinion OJT will > > become more common than it already is. ?Does anyone have an 'official' > > training program? ?Requirements to pass the exam? ?Qualifications to be > > able to be trained on-the-job? ?I'd like to consider having some kind of > > plan in place when I don't have an HT/HTL applicant but have folks who, > if > > they get the experience, are otherwise qualified to sit for the exam. ?If > > anyone has a similar situation or experience to share I would appreciate > > it! > > > > Thanks, > > Mike > > > > Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | > > Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | > > mjdessoye at commonwealthhealth.net > > > | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | > Fax: > > 570-552-1486 > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Toni.Rathborne at rwjuh.edu Mon May 18 14:11:10 2015 From: Toni.Rathborne at rwjuh.edu (Rathborne, Toni) Date: Mon, 18 May 2015 19:11:10 +0000 Subject: [Histonet] embedding center backup In-Reply-To: References: Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F2401A2FA9C@vap1014.win.rwjuh.edu> Stacy, Our volume is similar, and we do have a backup embedding center. I'm sure that you could get a used one for much less than a new one to have just for this purpose. Toni -----Original Message----- From: Stacy McLaughlin [mailto:Stacy_McLaughlin at cooley-dickinson.org] Sent: Monday, May 18, 2015 2:59 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] embedding center backup Happy Monday in Histoland, How many of you out there have a backup embedding center or paraffin dispenser for a backup? We are a smaller lab (<10k surgical cases/year). We only have one embedding center and I want to ask what others out there are doing. Thank you, Stacy Stacy McLaughlin, HT(ASCP) Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton,MA 01060 Stacy_McLaughlin at Cooley-Dickinson.org [Cooley-Dickinson.org] _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver at hotmail.com Mon May 18 14:56:02 2015 From: joelleweaver at hotmail.com (Joelle Weaver) Date: Mon, 18 May 2015 19:56:02 +0000 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: References: <47E9B2C01DDDD94881EACD2DC44EBC882247AACE@D1PWPEXMBX05.mdanderson.edu>, , , Message-ID: Research is a different world than "clinical". That's not fun. Can you pursue your degree if you haven't already and then do a 1 year under a clinical pathologist? It took me awhile to my clinical hours since I working full time in pharmacy and in histology school fulltime, but it was worth it. Joelle Weaver MAOM, HTL (ASCP) QIHC Date: Mon, 18 May 2015 11:59:59 -0700 Subject: Re: [Histonet] OJT Histotechs/Training From: lldewe at gmail.com To: joelleweaver at hotmail.com CC: jmacdonald at mtsac.edu; tnmayer at mdanderson.org; histonet at lists.utsouthwestern.edu I have a different perspective on this issue. I have been in histology for over 20 years. I worked at UC Davis in Vet. Histopath for several years. I was a histology Core facility manager and started up the facility from scratch at UC Davis Health system while running a Core Confocal microscope facility there. BUT I was in research, I wasn't in a "Pathology lab" and I don't qualify for the HT or HTL so I can't get work in the industry. Talk about a conundrum! Loralei On Sun, May 17, 2015 at 3:38 AM, Joelle Weaver wrote: I will speak to my laboratory director about this. I know the situation first hand from my previous experience! Joelle Weaver MAOM, HTL (ASCP) QIHC > To: TNMayer at mdanderson.org > From: JMacDonald at mtsac.edu > Date: Sat, 16 May 2015 20:02:34 -0700 > Subject: Re: [Histonet] OJT Histotechs/Training > CC: histonet at lists.utsouthwestern.edu > > This is an issue with our program as well. We have a difficult time > finding clinical sites for our students. Many people want to hire trained > individuals, but don't want to invest any time in the training. Our > students receive a great deal of hands-on time in the student laboratory, > but need "real life" experience. > Jennifer MacDonald > Mt. San Antonio College > > > > From: "Mayer,Toysha N" > To: "'histonet at lists.utsouthwestern.edu'" > > Date: 05/14/2015 01:48 PM > Subject: Re: [Histonet] OJT Histotechs/Training > > > > One good way to find techs is to offer to become a clinical affiliate for > a program. Most programs struggle with attracting students and providing > them with clinical affiliates to fine tune their skills. > It may not matter that the school is not located near you, the student may > have family nearby to stay with. > We are always looking for long distance affiliates, that way we can > attract an out-of-state student and not saturate the local area. I have > students who want to relocate to different areas and just for a change and > this helps them do so. We also get calls from applicants who don't mind > moving to us for 9-10 months, as long as they can go home when they > finish. > If the program is agreeable to this, the specifics can be worked out, such > as what skills are entry level and the length of the time the student is > at your facility. > Ours is called an Internship and the student is at the facility for 12 > weeks. They come in knowing basic embedding, cutting, routine staining, > specials, and have performed a minimum of three IHC stains. Two are > manual and one automated. > Some programs keep the students in house for some time before they leave > for internship, while others leave the technical training to the clinics. > It all depends on what is available. > This would be a low cost way to see if you like a person, can train them > and are willing to teach. > Some students are looking to relocate just before graduation, so a move > for an internship is a consideration. > Many times it is the expectations of the trainer that are not aligned with > the skill level of entry-level techs and that can cause problems. This > way the person can come in with an assessment of the skill level and the > OJT phase can begin. If the affiliate chooses to hire the student, great. > If not, then no harm. At least you get to say that you tried and did not > have to waste money doing so. It is not a source of free labor, but a way > of accurately assessing a person's fit for your needs. > Many allied health programs (not just histo) are doing this and it helps > to showcase different labs and programs. > > Just my two cents. > > Sincerely, > > Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) > Instructor/Education Coordinator > Program in Histotechnology > School of Health Professions > UT M.D. Anderson Cancer Center > 713.563-3481 > > > > > > Message: 1 > Date: Thu, 14 May 2015 17:07:06 +0000 > From: "Morken, Timothy" > To: Pam Marcum , Lisa Roy > Cc: Histonet , Michael Dessoye > > Subject: Re: [Histonet] OJT Histotechs/Training > Message-ID: > <761E2B5697F795489C8710BCC72141FF36831E99 at ex07.net.ucsf.edu> > Content-Type: text/plain; charset="utf-8" > > I think there is some actor from the CSI series that has done some of this > work promoting lab techs... > > Tim Morken > > -----Original Message----- > From: Pam Marcum [mailto:mucram11 at comcast.net] > Sent: Thursday, May 14, 2015 9:18 AM > To: Lisa Roy > Cc: Histonet; Michael Dessoye > Subject: Re: [Histonet] OJT Histotechs/Training > > I understand and agree with everything being said and feel we do need more > education in getting your registry, as Histology is changing and > growing.??We need to be prepared to grow with it, much as we did when IHC > first came into Histology and many thought it would go to the MTs.?? > ? > The one thing that has not changed in the 50 years I have done Histology > is the fact that no one outside of AP knows what a Histologist is or what > we do.? (I'm tried of being asked "Oh what kind of history is that?")? > Until we change that and get more information about the field and > advantages we will still be in the straights we are in now.? No one > joining because so few people even know what we do or that there is an > opportunity here.? If you don't know what Histology is why would you even > look at the field.? I know about and have done school visits, career days > etc; and those are not enough.? > ? > We need a spokesperson or celebrity?who has needed our services and not > even known we, Histology, were the ones who did the slides their wonderful > doctors used to save their lives.? This person or persons needs to speak > loud and strong the way Robin Roberts has done on TV for her doctors > and?help.?However; Histology was neven mentioned in those gratis > moments.?I have only known one?person in NSH who suggested this and no one > listened.? If?they can't see you or know you - you don't exist.??Can we > all take off the blinders and?look at what we need in publicity and stop > waiting for NSH and ASCP to do it.???Then we can offer these possible > future HTs and HTLs something, like being recognized as full laboratory > professionals and a higher level of lab aide. > ? > Just my thoughts (for many years and spoken often) > ? > Pam Marcum > > ----- Original Message ----- > > From: "Lisa Roy" > To: "Michael Dessoye" , "Histonet" > > Sent: Thursday, May 14, 2015 7:55:19 AM > Subject: Re: [Histonet] OJT Histotechs/Training > > I currently have 3 open tech positions and don't have any qualified > applicants applying for the job. ?I have recently taken a lab aide that > showed interest and aptitude and began OJT. ?With less than 30 schools in > the country actually teaching histology, this is one day going to be the > way. ?Already having a bachelors in biology, my aide qualifies to sit for > the ASCP exam once he has completed one full year of tech work and has a > pathologist willing to review his work and sign off on the ASCP paperwork. > ?Without going through a traditional program, one must have an associates > or bachelor's degree with a ?certain amount of Chemistry and Science > credits. ?As far as the training, I started with embedding and moved on > from there to cutting and then special staining. ?All along way, working > on troubleshooting and documenting EVERYTHING. ?Some places will hire > someone with only a high school diploma as long as they have previous HT > experience. ?I think the specifics of what each in > stitution would deem a qualified trainee will vary from place to place. > ?Smaller hospitals or labs may be okay training someone with aptitude that > doesn't necessarily fit the ASCP exam qualifications, but large > corporations might really insist that the trainee be certifiable at some > point. > > Frankly, I think taking someone that shows an interest and has the > knowledge to be a great tech is better than hiring someone that you may > not know what you are getting. ?Doing OJT ensures that you are teaching > the candidate exactly how you want things done and not having to accept > the bad habits of someone that has been doing it a long time and set in > their own ways. > > Good luck > Lisa ? ? > > -----Original Message----- > From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] > Sent: Thursday, May 14, 2015 7:44 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] OJT Histotechs/Training > > Hello Histonet, > > I'm curious how people are dealing with on-the-job-trained histotechs. > ?Many people are seeing a shortage in techs, and in my opinion OJT will > become more common than it already is. ?Does anyone have an 'official' > training program? ?Requirements to pass the exam? ?Qualifications to be > able to be trained on-the-job? ?I'd like to consider having some kind of > plan in place when I don't have an HT/HTL applicant but have folks who, if > they get the experience, are otherwise qualified to sit for the exam. ?If > anyone has a similar situation or experience to share I would appreciate > it! > > Thanks, > Mike > > Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | > Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | > mjdessoye at commonwealthhealth.net > | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: > 570-552-1486 > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum at uams.edu Mon May 18 15:31:40 2015 From: PAMarcum at uams.edu (Marcum, Pamela A) Date: Mon, 18 May 2015 20:31:40 +0000 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: References: <47E9B2C01DDDD94881EACD2DC44EBC882247AACE@D1PWPEXMBX05.mdanderson.edu> Message-ID: I took my registry while I was doing neuroanatomical research on animals. At the time I took it I was told do not use animal tissue or you will fail. I had to find someone with a clinical Histology lab who would allow me to come in for my practical exam work. I just had to prove myself when I wanted to get into clinical by working for a little less with the agreement that if I met the standard the pay and position would go up. However; without the HT I would never have gotten through the door. We have research people here at UAMS who are taking the exam because their PIs are willing to help them with a pathologist and have their work looked at for quality. There are ways around the rules we sometimes have to bend a little. Pam Marcum -----Original Message----- From: Loralei Dewe [mailto:lldewe at gmail.com] Sent: Monday, May 18, 2015 2:00 PM To: Joelle Weaver Cc: histonet at lists.utsouthwestern.edu; Mayer, Toysha N Subject: Re: [Histonet] OJT Histotechs/Training I have a different perspective on this issue. I have been in histology for over 20 years. I worked at UC Davis in Vet. Histopath for several years. I was a histology Core facility manager and started up the facility from scratch at UC Davis Health system while running a Core Confocal microscope facility there. BUT I was in research, I wasn't in a "Pathology lab" and I don't qualify for the HT or HTL so I can't get work in the industry. Talk about a conundrum! Loralei On Sun, May 17, 2015 at 3:38 AM, Joelle Weaver wrote: > I will speak to my laboratory director about this. I know the > situation first hand from my previous experience! > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > > > > To: TNMayer at mdanderson.org > > From: JMacDonald at mtsac.edu > > Date: Sat, 16 May 2015 20:02:34 -0700 > > Subject: Re: [Histonet] OJT Histotechs/Training > > CC: histonet at lists.utsouthwestern.edu > > > > This is an issue with our program as well. We have a difficult time > > finding clinical sites for our students. Many people want to hire > trained > > individuals, but don't want to invest any time in the training. Our > > students receive a great deal of hands-on time in the student > > laboratory, but need "real life" experience. > > Jennifer MacDonald > > Mt. San Antonio College > > > > > > > > From: "Mayer,Toysha N" > > To: "'histonet at lists.utsouthwestern.edu'" > > > > Date: 05/14/2015 01:48 PM > > Subject: Re: [Histonet] OJT Histotechs/Training > > > > > > > > One good way to find techs is to offer to become a clinical > > affiliate for a program. Most programs struggle with attracting > > students and providing them with clinical affiliates to fine tune their skills. > > It may not matter that the school is not located near you, the > > student > may > > have family nearby to stay with. > > We are always looking for long distance affiliates, that way we can > > attract an out-of-state student and not saturate the local area. I > > have students who want to relocate to different areas and just for a > > change > and > > this helps them do so. We also get calls from applicants who don't > > mind moving to us for 9-10 months, as long as they can go home when > > they finish. > > If the program is agreeable to this, the specifics can be worked > > out, > such > > as what skills are entry level and the length of the time the > > student is at your facility. > > Ours is called an Internship and the student is at the facility for > > 12 weeks. They come in knowing basic embedding, cutting, routine > > staining, specials, and have performed a minimum of three IHC > > stains. Two are manual and one automated. > > Some programs keep the students in house for some time before they > > leave for internship, while others leave the technical training to the clinics. > > It all depends on what is available. > > This would be a low cost way to see if you like a person, can train > > them and are willing to teach. > > Some students are looking to relocate just before graduation, so a > > move for an internship is a consideration. > > Many times it is the expectations of the trainer that are not > > aligned > with > > the skill level of entry-level techs and that can cause problems. > > This way the person can come in with an assessment of the skill > > level and the OJT phase can begin. If the affiliate chooses to hire > > the student, > great. > > If not, then no harm. At least you get to say that you tried and > > did > not > > have to waste money doing so. It is not a source of free labor, but > > a > way > > of accurately assessing a person's fit for your needs. > > Many allied health programs (not just histo) are doing this and it > > helps to showcase different labs and programs. > > > > Just my two cents. > > > > Sincerely, > > > > Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education > > Coordinator Program in Histotechnology School of Health Professions > > UT M.D. Anderson Cancer Center > > 713.563-3481 > > > > > > > > > > > > Message: 1 > > Date: Thu, 14 May 2015 17:07:06 +0000 > > From: "Morken, Timothy" > > To: Pam Marcum , Lisa Roy > > Cc: Histonet , Michael Dessoye > > > > Subject: Re: [Histonet] OJT Histotechs/Training > > Message-ID: > > <761E2B5697F795489C8710BCC72141FF36831E99 at ex07.net.ucsf.edu> > > Content-Type: text/plain; charset="utf-8" > > > > I think there is some actor from the CSI series that has done some > > of > this > > work promoting lab techs... > > > > Tim Morken > > > > -----Original Message----- > > From: Pam Marcum [mailto:mucram11 at comcast.net] > > Sent: Thursday, May 14, 2015 9:18 AM > > To: Lisa Roy > > Cc: Histonet; Michael Dessoye > > Subject: Re: [Histonet] OJT Histotechs/Training > > > > I understand and agree with everything being said and feel we do > > need > more > > education in getting your registry, as Histology is changing and > > growing.??We need to be prepared to grow with it, much as we did > > when IHC first came into Histology and many thought it would go to the MTs.?? > > ? > > The one thing that has not changed in the 50 years I have done > > Histology is the fact that no one outside of AP knows what a > > Histologist is or what we do.? (I'm tried of being asked "Oh what kind of history is that?")? > > Until we change that and get more information about the field and > > advantages we will still be in the straights we are in now.? No one > > joining because so few people even know what we do or that there is > > an opportunity here.? If you don't know what Histology is why would > > you even look at the field.? I know about and have done school > > visits, career days etc; and those are not enough.? > > ? > > We need a spokesperson or celebrity?who has needed our services and > > not even known we, Histology, were the ones who did the slides their > wonderful > > doctors used to save their lives.? This person or persons needs to > > speak loud and strong the way Robin Roberts has done on TV for her > > doctors and?help.?However; Histology was neven mentioned in those > > gratis moments.?I have only known one?person in NSH who suggested > > this and no > one > > listened.? If?they can't see you or know you - you don't exist.??Can > > we all take off the blinders and?look at what we need in publicity > > and stop waiting for NSH and ASCP to do it.???Then we can offer > > these possible future HTs and HTLs something, like being recognized > > as full laboratory professionals and a higher level of lab aide. > > ? > > Just my thoughts (for many years and spoken often) ? > > Pam Marcum > > > > ----- Original Message ----- > > > > From: "Lisa Roy" > > To: "Michael Dessoye" , "Histonet" > > > > Sent: Thursday, May 14, 2015 7:55:19 AM > > Subject: Re: [Histonet] OJT Histotechs/Training > > > > I currently have 3 open tech positions and don't have any qualified > > applicants applying for the job. ?I have recently taken a lab aide > > that showed interest and aptitude and began OJT. ?With less than 30 > > schools in the country actually teaching histology, this is one day > > going to be the way. ?Already having a bachelors in biology, my aide > > qualifies to sit for the ASCP exam once he has completed one full > > year of tech work and has a pathologist willing to review his work > > and sign off on the ASCP > paperwork. > > ?Without going through a traditional program, one must have an > > associates or bachelor's degree with a ?certain amount of Chemistry > > and Science credits. ?As far as the training, I started with > > embedding and moved on from there to cutting and then special > > staining. ?All along way, working on troubleshooting and documenting > > EVERYTHING. ?Some places will hire someone with only a high school > > diploma as long as they have previous HT experience. ?I think the > > specifics of what each in stitution would deem a qualified trainee will vary from place to place. > > ?Smaller hospitals or labs may be okay training someone with > > aptitude > that > > doesn't necessarily fit the ASCP exam qualifications, but large > > corporations might really insist that the trainee be certifiable at > > some point. > > > > Frankly, I think taking someone that shows an interest and has the > > knowledge to be a great tech is better than hiring someone that you > > may not know what you are getting. ?Doing OJT ensures that you are > > teaching the candidate exactly how you want things done and not > > having to accept the bad habits of someone that has been doing it a > > long time and set in their own ways. > > > > Good luck > > Lisa ? ? > > > > -----Original Message----- > > From: Dessoye, Michael [mailto:mjdessoye at commonwealthhealth.net] > > Sent: Thursday, May 14, 2015 7:44 AM > > To: histonet at lists.utsouthwestern.edu > > Subject: [Histonet] OJT Histotechs/Training > > > > Hello Histonet, > > > > I'm curious how people are dealing with on-the-job-trained histotechs. > > ?Many people are seeing a shortage in techs, and in my opinion OJT > > will become more common than it already is. ?Does anyone have an 'official' > > training program? ?Requirements to pass the exam? ?Qualifications to > > be able to be trained on-the-job? ?I'd like to consider having some > > kind of plan in place when I don't have an HT/HTL applicant but have > > folks who, > if > > they get the experience, are otherwise qualified to sit for the > > exam. ?If anyone has a similar situation or experience to share I > > would appreciate it! > > > > Thanks, > > Mike > > > > Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | > > Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health > > | > > mjdessoye at commonwealthhealth.net > .net > > > > | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | > Fax: > > 570-552-1486 > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From yesyes at comcast.net Mon May 18 18:22:59 2015 From: yesyes at comcast.net (yesyes at comcast.net) Date: Mon, 18 May 2015 23:22:59 +0000 (UTC) Subject: [Histonet] instrument manuals In-Reply-To: <1517687727.3626682.1431990981527.JavaMail.zimbra@comcast.net> Message-ID: <1705762184.3629747.1431991379767.JavaMail.zimbra@comcast.net> Hi, I'm doing some labkeeping and fould some old instrument manuals.? If anyone is interested in them, let me know and I can send them to you. ? Shandon Embedding Center Model 64000004? - old model Leica RM 2125? Rotary microtome Hyperclean Fume extraction hood Tissue Tek VIP 5 operating manual Leica Autostainer XL Reference Manual Leica Autostainer XL Instruction Manual Protocol MicroProbe staining system Tissue Tek TEC 5 Tissue embedder ? ? ? From yesyes at comcast.net Mon May 18 18:24:45 2015 From: yesyes at comcast.net (yesyes at comcast.net) Date: Mon, 18 May 2015 23:24:45 +0000 (UTC) Subject: [Histonet] Fwd: instrument manuals In-Reply-To: <1705762184.3629747.1431991379767.JavaMail.zimbra@comcast.net> References: <1705762184.3629747.1431991379767.JavaMail.zimbra@comcast.net> Message-ID: <234907699.3630471.1431991485651.JavaMail.zimbra@comcast.net> ----- Forwarded Message ----- From: yesyes at comcast.net To: histonet at lists.utsouthwestern.edu Sent: Monday, May 18, 2015 7:22:59 PM Subject: instrument manuals Hi, I'm doing some labkeeping and fould some old instrument manuals.? If anyone is interested in them, let me know and I can send them to you. ? Shandon Embedding Center Model 64000004? - old model Leica RM 2125? Rotary microtome Hyperclean Fume extraction hood Tissue Tek VIP 5 operating manual Leica Autostainer XL Reference Manual Leica Autostainer XL Instruction Manual Protocol MicroProbe staining system Tissue Tek TEC 5 Tissue embedder ? ? ? From Richard.Cartun at hhchealth.org Mon May 18 20:40:38 2015 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 19 May 2015 01:40:38 +0000 Subject: [Histonet] RSV+ tissue Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E3EE99B63@HHCEXCHMB03.hhcsystem.org> We recently performed an autopsy on a patient who died of complications related to Respiratory Syncytial Virus (RSV). We were able to label RSV in the formalin-fixed lung tissue using immunohistochemistry (or what I now call "Morphologic Proteomics"). I can provide unstained slides (maybe even a paraffin block) for those of you who would find this type of specimen useful. If interested, please tell me why you are interested, and provide your name, complete mailing address, telephone number, and a FedEx account number for shipping. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From joost.bruijntjes at tno.triskelion.nl Tue May 19 07:57:59 2015 From: joost.bruijntjes at tno.triskelion.nl (Bruijntjes, J.P. (Joost)) Date: Tue, 19 May 2015 12:57:59 +0000 Subject: [Histonet] (no subject) Message-ID: Thanks to all who responded on my request for CD-4; CD-8a and CD68 staining on mouse formalin fixed, paraffin embedded tissues Joost Bruijntjes From Finlay.Finlay at glasgow.ac.uk Tue May 19 09:02:16 2015 From: Finlay.Finlay at glasgow.ac.uk (Finlay Finlay) Date: Tue, 19 May 2015 14:02:16 +0000 Subject: [Histonet] training log Message-ID: <0164C4117A8D594DBBF5265626928E390AC24C@FORMED-ES.formed.internal> Hello all, Does anyone have a training log or competency list that they would be willing to share with me for an absolute beginner to hands on histology. I have previously trained people with limited experience but never from scratch (straight from university). I am keen not to miss the basic lab skills that I've previously taken for granted. Thank you in advance Finlay Finlay Senior Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.finlay at glasgow.ac.uk The University of Glasgow Charity Number SC004401 Disclaimer: This e-mail is intended for the recipient only. If you are not the intended recipient you must not use, disclose, distribute, copy, print, or rely upon this e-mail. If an addressing or transmission error has misdirected this e-mail, please notify the author by replying to this e-mail and delete any local copy from your machine. E-mails are not necessarily secure. Forensic Medicine & Science (FMS) does not accept responsibility for changes made to this message after it was sent. Opinions, conclusions and other information in this message expressed by others or which do not relate to the official business of FMS shall be understood as neither given nor endorsed by FMS. It is expressly declared that this e-mail does not constitute nor form part of any contract or unilateral obligation unless the contrary is specifically stated in this e-mail and authorised by FMS. From histology81176 at att.net Tue May 19 09:42:23 2015 From: histology81176 at att.net (Histology Technician) Date: Tue, 19 May 2015 14:42:23 +0000 (UTC) Subject: [Histonet] training log In-Reply-To: <0164C4117A8D594DBBF5265626928E390AC24C@FORMED-ES.formed.internal> References: <0164C4117A8D594DBBF5265626928E390AC24C@FORMED-ES.formed.internal> Message-ID: <610982354.946524.1432046543103.JavaMail.yahoo@mail.yahoo.com> I'd be interested in this too if anyone is willing to share.Thanks! On Tuesday, May 19, 2015 9:03 AM, Finlay Finlay wrote: Hello all, Does anyone have a training log or competency list that they would be willing to share with me for an absolute beginner to hands on histology. I have previously trained people with limited experience but never from scratch (straight from university). I am keen not to miss the basic lab skills that I've previously taken for granted. Thank you in advance Finlay Finlay Senior Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.finlay at glasgow.ac.uk The University of Glasgow Charity Number SC004401 Disclaimer: This e-mail is intended for the recipient only. If you are not the intended recipient you must not use, disclose, distribute, copy, print, or rely upon this e-mail. If an addressing or transmission error has misdirected this e-mail, please notify the author by replying to this e-mail and delete any local copy from your machine. E-mails are not necessarily secure. Forensic Medicine & Science (FMS) does not accept responsibility for changes made to this message after it was sent. Opinions, conclusions and other information in this message expressed by others or which do not relate to the official business of FMS shall be understood as neither given nor endorsed by FMS. It is expressly declared that this e-mail does not constitute nor form part of any contract or unilateral obligation unless the contrary is specifically stated in this e-mail and authorised by FMS. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dennis.Hahn at cookchildrens.org Tue May 19 09:51:33 2015 From: Dennis.Hahn at cookchildrens.org (Dennis Hahn) Date: Tue, 19 May 2015 14:51:33 +0000 Subject: [Histonet] HT Open position Message-ID: Cook Children's Medical Center in Fort Worth, TX has an immediate opening for a Full Time HT. Hours will be Monday-Friday 9a-530pm. No weekends or holidays at this time. Our team consists of 5 HT's, 1 PA and 6.5 pathologists. We are a small but very busy Histology Laboratory. We are currently planning an entire new laboratory to open in February 2016. Interested candidates must apply at cookchildrens.org, However, feel free to contact me with any additional questions about the position. Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Laboratory Safety Officer Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 From dellav at musc.edu Tue May 19 09:57:37 2015 From: dellav at musc.edu (della Speranza, Vinnie) Date: Tue, 19 May 2015 14:57:37 +0000 Subject: [Histonet] Dr. Salah Deeb ? Message-ID: If there is anyone on this list who can provide contact information for Dr. Salah Deeb from Egypt, please contact me off list. Thank you, Vinnie Della Speranza | Manager for Anatomic Pathology Services| Medical University of South Carolina | 165 Ashley Avenue MSC 908 | Charleston, South Carolina 29425 | Office: 843.792.6353 | Fax: 843.792.8974 | dellav at musc.edu From HernandezJW at uthscsa.edu Tue May 19 12:01:04 2015 From: HernandezJW at uthscsa.edu (Hernandez, Jesus Willibaldo) Date: Tue, 19 May 2015 17:01:04 +0000 Subject: [Histonet] Need help reviewing undecalcified bone slides Message-ID: Hi all, I just wanted to know if anyone out there had experience reviewing undecalcified bone sections. I still feel like the morphology is not showing up on my sections so I am in need of assistance in troubleshooting this issue. I just uploaded two pictures on the histonet server for review. You can also reply to me for more pictures or my methodology. Thank you once again. Regards, Jesse Hernandez From anna.coffey at nih.gov Tue May 19 13:00:46 2015 From: anna.coffey at nih.gov (Coffey, Anna (NIH/NCI) [C]) Date: Tue, 19 May 2015 18:00:46 +0000 Subject: [Histonet] OJT Histotechs/Training Message-ID: <5C3E10119A1B824FBE92B08279F74A910258E3A8@msgb10.nih.gov> Hi Loralei, Maybe I'm just misunderstanding, but I'm unclear why you would be ineligible to sit for the exam with your experience. I've always worked in a research lab on animal tissues and used that experience to qualify for the exam. There is no longer a practical portion of the exam (the entire thing is a multiple choice electronic exam) and none of the paperwork requires a pathologist to sign off as your supervisor (this is the form to verify experience: http://www.ascp.org/pdf/BOC-PDFs/Documentation-forms/DocumentationFormHTLRoute2.aspx). According to the ASCP site, experience counts as "one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years." It's my understanding that the exam requirements have changed over the last few years (before I got my certification) and maybe you now would be eligible for the certification? Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey at nih.gov 301-846-1730 Message: 3 Date: Mon, 18 May 2015 11:59:59 -0700 From: Loralei Dewe > To: Joelle Weaver > Cc: Jennifer MacDonald >, "Mayer, Toysha N" >, "histonet at lists.utsouthwestern.edu" > Subject: Re: [Histonet] OJT Histotechs/Training Message-ID: > Content-Type: text/plain; charset=UTF-8 I have a different perspective on this issue. I have been in histology for over 20 years. I worked at UC Davis in Vet. Histopath for several years. I was a histology Core facility manager and started up the facility from scratch at UC Davis Health system while running a Core Confocal microscope facility there. BUT I was in research, I wasn't in a "Pathology lab" and I don't qualify for the HT or HTL so I can't get work in the industry. Talk about a conundrum! Loralei From mundayscott at gmavt.net Tue May 19 13:38:11 2015 From: mundayscott at gmavt.net (scott munday) Date: Tue, 19 May 2015 14:38:11 -0400 Subject: [Histonet] Microscope Problems? Message-ID: *We have several Olympus BX40 and BX41 compound microscopes in stock! and offer a 1 year warranty on all scopes. * *All microscopes are fully refurbished and are priced at 40% off list. The scopes are serviced by an Authorized Olympus Service Tech before sold. Please Email with questions.* -- Munday Scientific Instrument Service 90 Misha Lane Sanford, NC 27330 From liz at premierlab.com Tue May 19 13:48:43 2015 From: liz at premierlab.com (Elizabeth Chlipala) Date: Tue, 19 May 2015 12:48:43 -0600 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: <5C3E10119A1B824FBE92B08279F74A910258E3A8@msgb10.nih.gov> References: <5C3E10119A1B824FBE92B08279F74A910258E3A8@msgb10.nih.gov> Message-ID: <14E2C6176416974295479C64A11CB9AE02230F9D8D0C@SBS2K8.premierlab.local> Experience may not be the issue she needs to have an associate's degree with the appropriate credit hours to sit for the HT. It does not matter where you get your experience it does not need to be in a clinical lab. I have had several techs sit for the HTL registry and IHC qualification with research experience and I myself signed for their experience. I also needed to write a supplemental letter to the BOR explaining my position and that as a contract histology based CRO we did not have a medical director, etc. Here is essentially what I wrote to the BOR this was back in 2004, this was for the IHC qualification but I had written a similar letter to the BOR for her HTL certification, I also wrote one for myself for the IHC qualification when I took that back in 2004 also. I'm writing this letter to request a special consideration for the Immunohistochemistry Qualification for my employee Amy XXXXXX. She has registered to take the Immunohistochemistry Qualification this spring. She has already received the information packet. I own a small contract histology laboratory called Premier Laboratory, LLC. I have attached copies of our Articles of Incorporation, Colorado Division of Workers' Compensation, IRS Employer Identification Number, Colorado Business Registration and Application for Employer Identification Number. We are a small lab, consisting of myself and Amy. We do not have a PhD, MD, or DVM on staff to vouch for myself or Amy's level of experience performing immunohistochemical stains. Amy has been employed by Premier Laboratory since Feb 1, 2003. Amy also worked for me at BolderPATH (another business that I was a part owner) from June 1, 2002 to Jan 31, 2003. During her employment at BolderPATH and Premier Laboratory the majority of her time was spent developing immunohistochemical protocols and performing various immunohistochemical applications. Some of the antibodies that Amy has created protocols for and performed for clients at Premier Laboratory include: BrdU, Ki-67, PCNA, cleaved caspase-3, GFAP, CD31, F4/80 (clone BM8), F4/80 (clone A3-1), complement C3, Crry/p65, GAP-43, Luciferase, NOS2, PSA, synaptophysin, smooth muscle actin, S-100, LCA, CD20, UchL-1, CD11b, CD14, CD13, CD105, MMP-1, MMP-7, FLK-1, plus several in-house antibodies for various applications. I have performed these antibodies utilizing a variety of fixatives, processing methods and detection systems utilizing both frozen and paraffin embedded material. I have attached to this letter the Immunohistochemistry (IHC) Qualification Reference, which I have filled out for Amy. If you have any questions or require any additional information please give myself or Amy a call at Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Coffey, Anna (NIH/NCI) [C] [mailto:anna.coffey at nih.gov] Sent: Tuesday, May 19, 2015 12:01 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] OJT Histotechs/Training Hi Loralei, Maybe I'm just misunderstanding, but I'm unclear why you would be ineligible to sit for the exam with your experience. I've always worked in a research lab on animal tissues and used that experience to qualify for the exam. There is no longer a practical portion of the exam (the entire thing is a multiple choice electronic exam) and none of the paperwork requires a pathologist to sign off as your supervisor (this is the form to verify experience: http://www.ascp.org/pdf/BOC-PDFs/Documentation-forms/DocumentationFormHTLRoute2.aspx). According to the ASCP site, experience counts as "one year full time acceptable experience in a histopathology (clinical, veterinary, industry or research) laboratory in the U.S., Canada or an accredited laboratory* within the last ten years." It's my understanding that the exam requirements have changed over the last few years (before I got my certification) and maybe you now would be eligible for the certification? Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey at nih.gov 301-846-1730 Message: 3 Date: Mon, 18 May 2015 11:59:59 -0700 From: Loralei Dewe > To: Joelle Weaver > Cc: Jennifer MacDonald >, "Mayer, Toysha N" >, "histonet at lists.utsouthwestern.edu" > Subject: Re: [Histonet] OJT Histotechs/Training Message-ID: > Content-Type: text/plain; charset=UTF-8 I have a different perspective on this issue. I have been in histology for over 20 years. I worked at UC Davis in Vet. Histopath for several years. I was a histology Core facility manager and started up the facility from scratch at UC Davis Health system while running a Core Confocal microscope facility there. BUT I was in research, I wasn't in a "Pathology lab" and I don't qualify for the HT or HTL so I can't get work in the industry. Talk about a conundrum! Loralei _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lldewe at gmail.com Tue May 19 13:57:47 2015 From: lldewe at gmail.com (Loralei Dewe) Date: Tue, 19 May 2015 11:57:47 -0700 Subject: [Histonet] OJT Histotechs/Training In-Reply-To: <5C3E10119A1B824FBE92B08279F74A910258E3A8@msgb10.nih.gov> References: <5C3E10119A1B824FBE92B08279F74A910258E3A8@msgb10.nih.gov> Message-ID: Perhaps things have changed now. It was a few years ago that I explored the HTL situation. At that time I had to have a pathologist that I worked under for at least a year sign me off. Maybe things have changed. I'll look into it! Thanks everyone!! Loralei On Tue, May 19, 2015 at 11:00 AM, Coffey, Anna (NIH/NCI) [C] < anna.coffey at nih.gov> wrote: > Hi Loralei, > > > > Maybe I'm just misunderstanding, but I'm unclear why you would be > ineligible to sit for the exam with your experience. I've always worked in > a research lab on animal tissues and used that experience to qualify for > the exam. There is no longer a practical portion of the exam (the entire > thing is a multiple choice electronic exam) and none of the paperwork > requires a pathologist to sign off as your supervisor (this is the form to > verify experience: > http://www.ascp.org/pdf/BOC-PDFs/Documentation-forms/DocumentationFormHTLRoute2.aspx). > According to the ASCP site, experience counts as "one year full time > acceptable experience in a histopathology (clinical, veterinary, industry > or research) laboratory in the U.S., Canada or an accredited laboratory* > within the last ten years." > > > > It's my understanding that the exam requirements have changed over the > last few years (before I got my certification) and maybe you now would be > eligible for the certification? > > Anna Coffey, MS, HTL(ASCP)CM > Histotechnologist > Center for Advanced Preclinical Research > Frederick National Laboratory for Cancer Research > Leidos Biomedical Research, Inc. > Bld 539, 224 > Frederick, Maryland 21702 > anna.coffey at nih.gov > 301-846-1730 > > > > > > Message: 3 > > Date: Mon, 18 May 2015 11:59:59 -0700 > > From: Loralei Dewe > > > To: Joelle Weaver joelleweaver at hotmail.com>> > > Cc: Jennifer MacDonald >, > "Mayer, Toysha N" > > >, " > histonet at lists.utsouthwestern.edu >" > > histonet at lists.utsouthwestern.edu>> > > Subject: Re: [Histonet] OJT Histotechs/Training > > Message-ID: > > < > CAGeGmmdJUmgOb-Rvd3mWv9ioqZ9gpkw2L2OUzorHBYRpyhnc+Q at mail.gmail.com CAGeGmmdJUmgOb-Rvd3mWv9ioqZ9gpkw2L2OUzorHBYRpyhnc+Q at mail.gmail.com>> > > Content-Type: text/plain; charset=UTF-8 > > > > I have a different perspective on this issue. I have been in histology > > for over 20 years. I worked at UC Davis in Vet. Histopath for several > > years. I was a histology Core facility manager and started up the facility > > from scratch at UC Davis Health system while running a Core Confocal > > microscope facility there. BUT I was in research, I wasn't in a "Pathology > > lab" and I don't qualify for the HT or HTL so I can't get work in the > > industry. Talk about a conundrum! > > > > Loralei > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From joelleweaver at hotmail.com Tue May 19 15:20:02 2015 From: joelleweaver at hotmail.com (Joelle Weaver) Date: Tue, 19 May 2015 20:20:02 +0000 Subject: [Histonet] training log In-Reply-To: <610982354.946524.1432046543103.JavaMail.yahoo@mail.yahoo.com> References: <0164C4117A8D594DBBF5265626928E390AC24C@FORMED-ES.formed.internal>, <610982354.946524.1432046543103.JavaMail.yahoo@mail.yahoo.com> Message-ID: The QMC committee with the NSH has a competency guideline document that we put together. It covers the CAP elements of competency, CLIA requirements/differences from CAP, elements of competency, how to document & measure. I finished it up late last year and believe Kim has posted it on behalf of the committee for members. Joelle Weaver MAOM, HTL (ASCP) QIHC > Date: Tue, 19 May 2015 14:42:23 +0000 > From: histology81176 at att.net > To: Finlay.Finlay at glasgow.ac.uk; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] training log > > I'd be interested in this too if anyone is willing to share.Thanks! > > > On Tuesday, May 19, 2015 9:03 AM, Finlay Finlay wrote: > > > Hello all, > > Does anyone have a training log or competency list that they would be willing to share with me for an absolute beginner to hands on histology. I have previously trained people with limited experience but never from scratch (straight from university). I am keen not to miss the basic lab skills that I've previously taken for granted. > > Thank you in advance > > Finlay Finlay > > Senior Histology Technician > Forensic Medicine and Science > School of Medicine > College of Medical, Veterinary and Life Sciences > University of Glasgow > Joseph Black building > > Direct Line: +44 (0) 141 3303443 > E-mail: finlay.finlay at glasgow.ac.uk > > The University of Glasgow Charity Number SC004401 > > Disclaimer: This e-mail is intended for the recipient only. If you are not the intended recipient you must not use, disclose, distribute, copy, print, or rely upon this e-mail. If an addressing or transmission error has misdirected this e-mail, please notify the author by replying to this e-mail and delete any local copy from your machine. E-mails are not necessarily secure. Forensic Medicine & Science (FMS) does not accept responsibility for changes made to this message after it was sent. Opinions, conclusions and other information in this message expressed by others or which do not relate to the official business of FMS shall be understood as neither given nor endorsed by FMS. It is expressly declared that this e-mail does not constitute nor form part of any contract or unilateral obligation unless the contrary is specifically stated in this e-mail and authorised by FMS. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology81176 at att.net Tue May 19 16:02:07 2015 From: histology81176 at att.net (Histology Technician) Date: Tue, 19 May 2015 21:02:07 +0000 (UTC) Subject: [Histonet] Co-path users logging Slides in Message-ID: <30335397.1112830.1432069327454.JavaMail.yahoo@mail.yahoo.com> ? Those of you who use Co-path, how do you log slides from outside hospitals?? I see where you log slide Send outs, do you log incoming slides the same way?? I'm trying to figure out how to keep track of my slide consult slides? - when I received them, how many I received, when I sent them back to the original hospital, etc... Thanks! From Joyce.Weems at emoryhealthcare.org Tue May 19 16:10:12 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Tue, 19 May 2015 21:10:12 +0000 Subject: [Histonet] Co-path users logging Slides in In-Reply-To: <30335397.1112830.1432069327454.JavaMail.yahoo@mail.yahoo.com> References: <30335397.1112830.1432069327454.JavaMail.yahoo@mail.yahoo.com> Message-ID: We accession them as a case and document what is received in the report. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Histology Technician [mailto:histology81176 at att.net] Sent: Tuesday, May 19, 2015 5:02 PM To: Histonet; histonet-request at lists.utsouthwestern.edu Subject: [Histonet] Co-path users logging Slides in Those of you who use Co-path, how do you log slides from outside hospitals? I see where you log slide Send outs, do you log incoming slides the same way? I'm trying to figure out how to keep track of my slide consult slides - when I received them, how many I received, when I sent them back to the original hospital, etc... Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From suetp918 at comcast.net Tue May 19 16:12:55 2015 From: suetp918 at comcast.net (Sue) Date: Tue, 19 May 2015 21:12:55 +0000 (UTC) Subject: [Histonet] Co-path users logging Slides in In-Reply-To: References: <30335397.1112830.1432069327454.JavaMail.yahoo@mail.yahoo.com> Message-ID: <997056589.1781918.1432069975370.JavaMail.zimbra@comcast.net> We actually make them part of the gross description ? TJUH From Bonnie.Whitaker at osumc.edu Tue May 19 16:15:26 2015 From: Bonnie.Whitaker at osumc.edu (Whitaker, Bonnie) Date: Tue, 19 May 2015 17:15:26 -0400 Subject: [Histonet] Co-path users logging Slides in In-Reply-To: References: <30335397.1112830.1432069327454.JavaMail.yahoo@mail.yahoo.com> Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670E176B5F6A@EXMBOX-VP05.OSUMC.EDU> Ditto. -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] Sent: Tuesday, May 19, 2015 5:10 PM To: Histology Technician; Histonet; histonet-request at lists.utsouthwestern.edu Subject: Re: [Histonet] Co-path users logging Slides in We accession them as a case and document what is received in the report. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Histology Technician [mailto:histology81176 at att.net] Sent: Tuesday, May 19, 2015 5:02 PM To: Histonet; histonet-request at lists.utsouthwestern.edu Subject: [Histonet] Co-path users logging Slides in Those of you who use Co-path, how do you log slides from outside hospitals? I see where you log slide Send outs, do you log incoming slides the same way? I'm trying to figure out how to keep track of my slide consult slides - when I received them, how many I received, when I sent them back to the original hospital, etc... Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIGaQ&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=pqTPp8uy4SZPC2ukX1XtMt4zf27HFYP0YpWXNB1ydh4&s=JmslAUrabESLzCgewqr1qSVwyFt9mQzmx-8DK88L298&e= ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIGaQ&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=pqTPp8uy4SZPC2ukX1XtMt4zf27HFYP0YpWXNB1ydh4&s=JmslAUrabESLzCgewqr1qSVwyFt9mQzmx-8DK88L298&e= From Timothy.Morken at ucsf.edu Tue May 19 16:15:49 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 19 May 2015 21:15:49 +0000 Subject: [Histonet] Co-path users logging Slides in In-Reply-To: References: <30335397.1112830.1432069327454.JavaMail.yahoo@mail.yahoo.com> Message-ID: <761E2B5697F795489C8710BCC72141FF36833A29@ex07.net.ucsf.edu> Same as Joyce, we accession as consult case (as a separate specimen class) and do the whole barcoding routine on outside blocks and slides. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] Sent: Tuesday, May 19, 2015 2:10 PM To: Histology Technician; Histonet; histonet-request at lists.utsouthwestern.edu Subject: Re: [Histonet] Co-path users logging Slides in We accession them as a case and document what is received in the report. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Histology Technician [mailto:histology81176 at att.net] Sent: Tuesday, May 19, 2015 5:02 PM To: Histonet; histonet-request at lists.utsouthwestern.edu Subject: [Histonet] Co-path users logging Slides in Those of you who use Co-path, how do you log slides from outside hospitals? I see where you log slide Send outs, do you log incoming slides the same way? I'm trying to figure out how to keep track of my slide consult slides - when I received them, how many I received, when I sent them back to the original hospital, etc... Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ruio7 at hotmail.com Wed May 20 00:11:09 2015 From: ruio7 at hotmail.com (Rui TAHARA) Date: Wed, 20 May 2015 14:11:09 +0900 Subject: [Histonet] dehydration time for a relatively large sample Message-ID: Hi, I am wondering if prolong dehydration time with 95 and 100% ethanol would brittle the sample for paraffin sectioning. I have been trying to section adult zebra finch beak and processed several samples, however, i failed to obtain a good section. It appears that the paraffin did not penetrated the tissue. This may be derived from incomplete dehydration and clearing before paraffin. Because i have read somewhere that if the sample was sitting in 95 and 100% ethanol too long it would be brittle and be teared when sectioned. I have processed only a beak (about 1 cmX0.5 mm). Also when is an appropriate time to use vacuum? I am afraid that if i used it at 100% ethanol, the ethanol would evaporate.... so far I have tried; fix overnight decalcified few days walk from water to 70% ethanol; overnight 85%, 95% (2times change) and 100 % (2 times change) ethanol; overnight clearing; 2 days paraffin (2 times change); overnight Any suggestion would be appreciated. Thank you in advance, rui From klaus.dern44 at gmail.com Wed May 20 08:16:08 2015 From: klaus.dern44 at gmail.com (Klaus Dern) Date: Wed, 20 May 2015 09:16:08 -0400 Subject: [Histonet] THICK AND THIN SECTIONS ? Message-ID: Do you have one of the following microtomes and the advance mechanism is worn out. ( too much play between spindle and spindlenut ) REICHERT/JUNG 2030 LEICA/JUNG 2035 LEICA 2025 LEICA 2030 Biocut LEICA RM 2125/2125 RT LEICA - CM 1850 Cryostat SAKURA SRM 200 You could be faced with purchasing a new Microtome. ( No parts availability ) Rather than replacing these excellent Instruments, I have a PERMANENT solution to fix this problem. Klaus Dern Phone: 706 635-8840 E-Mail: klaus.dern44 at gmail.com From ruio7 at hotmail.com Wed May 20 08:44:11 2015 From: ruio7 at hotmail.com (Rui TAHARA) Date: Wed, 20 May 2015 22:44:11 +0900 Subject: [Histonet] dehydration time for a relatively large sample In-Reply-To: <8A886B98D8F46141B553250B4B5A1343C4F19645@SVSLS103V223.regsj.intern> References: , <8A886B98D8F46141B553250B4B5A1343C4F19645@SVSLS103V223.regsj.intern> Message-ID: Hi, Thanks for prompt response. We unfortunately do not have a processor in our lab at university..The protocol i wrote was working in quail embryonic samples (just before hatching). I process the tissue manually and cannot process the tissue with strict time schedule. Thus, I need to leave a sample overnight at some point. I cut the zebra finch head into anterior (beak) and posterior (brain) region and mid-sagittally in both. so each tissue sample is about 0.5 X 1 X 1cm cube at maximum. I have also tried short time schedule compared the one i wrote in previous email, for similar sized sample (zebra finch beak). However, it never worked. Thus i prolonged the each step for the latest sample. It would be great if you could provide me the proper time schedule. rui > From: krns at regionsjaelland.dk > To: ruio7 at hotmail.com > Subject: SV: [Histonet] dehydration time for a relatively large sample > Date: Wed, 20 May 2015 06:18:24 +0000 > > Hi Rui > > Which processor unit do you use > > to me it seems like a wrong protocol. > > Maybe I can help you set up a better protocol - if you want - but then I need to know size og your tissue, processor unit and what kind of clearens you use. > > Kind regards > Karen > Supervisor tissue processing > Denmark > > -----Oprindelig meddelelse----- > Fra: Rui TAHARA [mailto:ruio7 at hotmail.com] > Sendt: Wednesday, May 20, 2015 7:11 AM > Til: histonet at lists.utsouthwestern.edu > Emne: [Histonet] dehydration time for a relatively large sample > > Hi, > > I am wondering if prolong dehydration time with 95 and 100% ethanol would brittle the sample for paraffin sectioning. > > I have been trying to section adult zebra finch beak and processed several samples, however, > i failed to obtain a good section. It appears that the paraffin did not penetrated the tissue. This may be derived from incomplete dehydration and clearing before paraffin. > Because i have read somewhere that if the sample was sitting in 95 and 100% ethanol too long it would be brittle and be teared when sectioned. I have processed only a beak (about 1 cmX0.5 mm). > Also when is an appropriate time to use vacuum? I am afraid that if i used it at 100% ethanol, the ethanol would evaporate.... > > so far I have tried; > fix overnight > decalcified few days > walk from water to 70% ethanol; overnight > 85%, 95% (2times change) and 100 % (2 times change) ethanol; overnight > clearing; 2 days > paraffin (2 times change); overnight > > Any suggestion would be appreciated. > Thank you in advance, > > rui > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson at gnf.org Wed May 20 11:11:10 2015 From: JWatson at gnf.org (James Watson) Date: Wed, 20 May 2015 16:11:10 +0000 Subject: [Histonet] THICK AND THIN SECTIONS ? In-Reply-To: References: Message-ID: I see that advertising is now allowed on the Histonet? James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson at gnf.org -----Original Message----- From: Klaus Dern [mailto:klaus.dern44 at gmail.com] Sent: Wednesday, May 20, 2015 6:16 AM To: histonet Subject: [Histonet] THICK AND THIN SECTIONS ? Do you have one of the following microtomes and the advance mechanism is worn out. ( too much play between spindle and spindlenut ) REICHERT/JUNG 2030 LEICA/JUNG 2035 LEICA 2025 LEICA 2030 Biocut LEICA RM 2125/2125 RT LEICA - CM 1850 Cryostat SAKURA SRM 200 You could be faced with purchasing a new Microtome. ( No parts availability ) Rather than replacing these excellent Instruments, I have a PERMANENT solution to fix this problem. Klaus Dern Phone: 706 635-8840 E-Mail: klaus.dern44 at gmail.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson at gnf.org Wed May 20 11:11:24 2015 From: JWatson at gnf.org (James Watson) Date: Wed, 20 May 2015 16:11:24 +0000 Subject: [Histonet] Microscope Problems? In-Reply-To: References: Message-ID: I see that advertising is now allowed on the Histonet? James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson at gnf.org -----Original Message----- From: scott munday [mailto:mundayscott at gmavt.net] Sent: Tuesday, May 19, 2015 11:38 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Microscope Problems? *We have several Olympus BX40 and BX41 compound microscopes in stock! and offer a 1 year warranty on all scopes. * *All microscopes are fully refurbished and are priced at 40% off list. The scopes are serviced by an Authorized Olympus Service Tech before sold. Please Email with questions.* -- Munday Scientific Instrument Service 90 Misha Lane Sanford, NC 27330 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jshelley at sanfordburnham.org Wed May 20 12:06:29 2015 From: jshelley at sanfordburnham.org (John Shelley) Date: Wed, 20 May 2015 17:06:29 +0000 Subject: [Histonet] Florida NSH members Message-ID: Hello Florida NSH Histonetters, I am in need of those who would like to be part of the NSH House of Delegates for the Florida Society. We are entitled to a certain number delegates who are NSH active members from the state of Florida. The annual meeting of the House of Delegates of the National Society for Histotechnology will convene on September 2, 2015 at 7:00 PM in Washington, DC. I need to submit this information as soon as possible and would ask if you contact me off-line in response to this request. Thanks!!! Kind Regards! John J Shelley 2014-2016 FSH President From amosbrooks at gmail.com Wed May 20 17:02:54 2015 From: amosbrooks at gmail.com (Amos Brooks) Date: Wed, 20 May 2015 18:02:54 -0400 Subject: [Histonet] training log Message-ID: Hi, Actually that would be cool for comparison purposes for me too. Perhaps it might be easiest to put them on dropbox and post a link to the histonet. But please share. Thanks, Amos Brooks On Tue, May 19, 2015 at 1:00 PM, wrote: > Message: 12 > Date: Tue, 19 May 2015 14:42:23 +0000 (UTC) > From: Histology Technician > To: Finlay Finlay , > "'histonet at lists.utsouthwestern.edu'" > > Subject: Re: [Histonet] training log > Message-ID: > <610982354.946524.1432046543103.JavaMail.yahoo at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > I'd be interested in this too if anyone is willing to share.Thanks! > > > On Tuesday, May 19, 2015 9:03 AM, Finlay Finlay < > Finlay.Finlay at glasgow.ac.uk> wrote: > > > Hello all, > > Does anyone have a training log or competency list that they would be > willing to share with me for an absolute beginner to hands on histology. I > have previously trained people with limited experience but never from > scratch (straight from university). I am keen not to miss the basic lab > skills that I've previously taken for granted. > > Thank you in advance > > Finlay Finlay > > Senior Histology Technician > Forensic Medicine and Science > School of Medicine > From abtdhu at gmail.com Wed May 20 17:33:01 2015 From: abtdhu at gmail.com (abtdhu at gmail.com) Date: Wed, 20 May 2015 18:33:01 -0400 Subject: [Histonet] Histonet Digest, Vol 138, Issue 24 In-Reply-To: References: Message-ID: <4164A2E3-13C2-4768-8659-3D4EFAEA6E76@gmail.com> Where are the photos? Can we upload photo? > On May 20, 2015, at 1:00 PM, histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Need help reviewing undecalcified bone slides > (Hernandez, Jesus Willibaldo) > 2. OJT Histotechs/Training (Coffey, Anna (NIH/NCI) [C]) > 3. Microscope Problems? (scott munday) > 4. Re: OJT Histotechs/Training (Elizabeth Chlipala) > 5. Re: OJT Histotechs/Training (Loralei Dewe) > 6. Re: training log (Joelle Weaver) > 7. Co-path users logging Slides in (Histology Technician) > 8. Re: Co-path users logging Slides in (Weems, Joyce K.) > 9. Re: Co-path users logging Slides in (Sue) > 10. Re: Co-path users logging Slides in (Whitaker, Bonnie) > 11. Re: Co-path users logging Slides in (Morken, Timothy) > 12. dehydration time for a relatively large sample (Rui TAHARA) > 13. THICK AND THIN SECTIONS ? (Klaus Dern) > 14. Re: dehydration time for a relatively large sample (Rui TAHARA) > 15. Re: THICK AND THIN SECTIONS ? (James Watson) > 16. Re: Microscope Problems? (James Watson) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 19 May 2015 17:01:04 +0000 > From: "Hernandez, Jesus Willibaldo" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Need help reviewing undecalcified bone slides > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Hi all, > > I just wanted to know if anyone out there had experience reviewing undecalcified bone sections. I still feel like the morphology is not showing up on my sections so I am in need of assistance in troubleshooting this issue. I just uploaded two pictures on the histonet server for review. You can also reply to me for more pictures or my methodology. Thank you once again. > > Regards, > > > From abtdhu at gmail.com Wed May 20 17:35:16 2015 From: abtdhu at gmail.com (abtdhu at gmail.com) Date: Wed, 20 May 2015 18:35:16 -0400 Subject: [Histonet] What kind of undecalcified bone, mama or frozen, human or mouse? Message-ID: <06E0A1A4-7330-4F6D-8FDB-0E29790A8799@gmail.com> Message: 1 Date: Tue, 19 May 2015 17:01:04 +0000 From: "Hernandez, Jesus Willibaldo" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Need help reviewing undecalcified bone slides Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, I just wanted to know if anyone out there had experience reviewing undecalcified bone sections. I still feel like the morphology is not showing up on my sections so I am in need of assistance in troubleshooting this issue. I just uploaded two pictures on the histonet server for review. You can also reply to me for more pictures or my methodology. Thank you once again. Regards, From mhanna at histosearch.com Wed May 20 21:18:31 2015 From: mhanna at histosearch.com (mhanna at histosearch.com) Date: Wed, 20 May 2015 22:18:31 -0400 Subject: [Histonet] Uploading images Message-ID: <000001d0936c$6f8061a0$4e8124e0$@histosearch.com> You may upload images at http://histosearch.com/imageupload/. Attachments are not allowed on the Histonet list server. The images on undecalcified bone sections that Jesse uploaded are at: http://histosearch.com/imageupload/undecalcified-human-bone-he-stain-8-micro ns/ http://histosearch.com/imageupload/undecalcified-human-bone-he-stain-5-micro ns-3/ Let me know if you have any questions or problems. Marvin -----Original Message----- From: abtdhu at gmail.com [mailto:abtdhu at gmail.com] Sent: Wednesday, May 20, 2015 6:33 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 138, Issue 24 Where are the photos? Can we upload photo? > On May 20, 2015, at 1:00 PM, histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Need help reviewing undecalcified bone slides > (Hernandez, Jesus Willibaldo) > 2. OJT Histotechs/Training (Coffey, Anna (NIH/NCI) [C]) > 3. Microscope Problems? (scott munday) > 4. Re: OJT Histotechs/Training (Elizabeth Chlipala) > 5. Re: OJT Histotechs/Training (Loralei Dewe) > 6. Re: training log (Joelle Weaver) > 7. Co-path users logging Slides in (Histology Technician) > 8. Re: Co-path users logging Slides in (Weems, Joyce K.) > 9. Re: Co-path users logging Slides in (Sue) 10. Re: Co-path users > logging Slides in (Whitaker, Bonnie) 11. Re: Co-path users logging > Slides in (Morken, Timothy) 12. dehydration time for a relatively > large sample (Rui TAHARA) 13. THICK AND THIN SECTIONS ? (Klaus Dern) > 14. Re: dehydration time for a relatively large sample (Rui TAHARA) > 15. Re: THICK AND THIN SECTIONS ? (James Watson) 16. Re: Microscope > Problems? (James Watson) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 19 May 2015 17:01:04 +0000 > From: "Hernandez, Jesus Willibaldo" < HernandezJW at uthscsa.edu> > To: " histonet at lists.utsouthwestern.edu" > < histonet at lists.utsouthwestern.edu> > Subject: [Histonet] Need help reviewing undecalcified bone slides > Message-ID: > > > > > Content-Type: text/plain; charset="us-ascii" > > Hi all, > > I just wanted to know if anyone out there had experience reviewing undecalcified bone sections. I still feel like the morphology is not showing up on my sections so I am in need of assistance in troubleshooting this issue. I just uploaded two pictures on the histonet server for review. You can also reply to me for more pictures or my methodology. Thank you once again. > > Regards, > > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhanna at histosearch.com Wed May 20 21:35:48 2015 From: mhanna at histosearch.com (mhanna at histosearch.com) Date: Wed, 20 May 2015 22:35:48 -0400 Subject: [Histonet] Uploading images Message-ID: <000d01d0936e$d9aa32c0$8cfe9840$@histosearch.com> The hyperlinks to Jesse's images got cut off in my email. If those links don't work, try these. Image 1 Image 2 Marvin -----Original Message----- From: mhanna at histosearch.com [mailto:mhanna at histosearch.com] Sent: Wednesday, May 20, 2015 10:19 PM To: abtdhu at gmail.com; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Uploading images You may upload images at http://histosearch.com/imageupload/. Attachments are not allowed on the Histonet list server. The images on undecalcified bone sections that Jesse uploaded are at: http://histosearch.com/imageupload/undecalcified-human-bone-he-stain-8-micro ns/ http://histosearch.com/imageupload/undecalcified-human-bone-he-stain-5-micro ns-3/ Let me know if you have any questions or problems. Marvin -----Original Message----- From: abtdhu at gmail.com [ mailto:abtdhu at gmail.com] Sent: Wednesday, May 20, 2015 6:33 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 138, Issue 24 Where are the photos? Can we upload photo? > On May 20, 2015, at 1:00 PM, < mailto:histonet-request at lists.utsouthwestern.edu> histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > < mailto:histonet at lists.utsouthwestern.edu> histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > < http://lists.utsouthwestern.edu/mailman/listinfo/histonet> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > < mailto:histonet-request at lists.utsouthwestern.edu> histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > < mailto:histonet-owner at lists.utsouthwestern.edu> histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Need help reviewing undecalcified bone slides > (Hernandez, Jesus Willibaldo) > 2. OJT Histotechs/Training (Coffey, Anna (NIH/NCI) [C]) > 3. Microscope Problems? (scott munday) > 4. Re: OJT Histotechs/Training (Elizabeth Chlipala) > 5. Re: OJT Histotechs/Training (Loralei Dewe) > 6. Re: training log (Joelle Weaver) > 7. Co-path users logging Slides in (Histology Technician) > 8. Re: Co-path users logging Slides in (Weems, Joyce K.) > 9. Re: Co-path users logging Slides in (Sue) 10. Re: Co-path users > logging Slides in (Whitaker, Bonnie) 11. Re: Co-path users logging > Slides in (Morken, Timothy) 12. dehydration time for a relatively > large sample (Rui TAHARA) 13. THICK AND THIN SECTIONS ? (Klaus Dern) > 14. Re: dehydration time for a relatively large sample (Rui TAHARA) > 15. Re: THICK AND THIN SECTIONS ? (James Watson) 16. Re: Microscope > Problems? (James Watson) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 19 May 2015 17:01:04 +0000 > From: "Hernandez, Jesus Willibaldo" < < mailto:HernandezJW at uthscsa.edu> HernandezJW at uthscsa.edu> > To: " < mailto:histonet at lists.utsouthwestern.edu> histonet at lists.utsouthwestern.edu" > < < mailto:histonet at lists.utsouthwestern.edu> histonet at lists.utsouthwestern.edu> > Subject: [Histonet] Need help reviewing undecalcified bone slides > Message-ID: > > > > > Content-Type: text/plain; charset="us-ascii" > > Hi all, > > I just wanted to know if anyone out there had experience reviewing undecalcified bone sections. I still feel like the morphology is not showing up on my sections so I am in need of assistance in troubleshooting this issue. I just uploaded two pictures on the histonet server for review. You can also reply to me for more pictures or my methodology. Thank you once again. > > Regards, > > > _______________________________________________ Histonet mailing list < mailto:Histonet at lists.utsouthwestern.edu> Histonet at lists.utsouthwestern.edu < http://lists.utsouthwestern.edu/mailman/listinfo/histonet> http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Finlay.Finlay at glasgow.ac.uk Thu May 21 03:48:29 2015 From: Finlay.Finlay at glasgow.ac.uk (Finlay Finlay) Date: Thu, 21 May 2015 08:48:29 +0000 Subject: [Histonet] training logs Message-ID: <0164C4117A8D594DBBF5265626928E390AC5E7@FORMED-ES.formed.internal> Thank you to everyone who has emailed me example training logs. A few people have requested that I share the results with them and it was suggested that I put up a dropbox link to all of those provided. I thought that this sounded like a good idea so I will do this later, if anyone would rather that I did not include their documents or if anyone else would like to add additional documents to this collection then please let me know today. Thanks again Finlay Finlay Senior Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.finlay at glasgow.ac.uk The University of Glasgow Charity Number SC004401 Disclaimer: This e-mail is intended for the recipient only. If you are not the intended recipient you must not use, disclose, distribute, copy, print, or rely upon this e-mail. If an addressing or transmission error has misdirected this e-mail, please notify the author by replying to this e-mail and delete any local copy from your machine. E-mails are not necessarily secure. Forensic Medicine & Science (FMS) does not accept responsibility for changes made to this message after it was sent. Opinions, conclusions and other information in this message expressed by others or which do not relate to the official business of FMS shall be understood as neither given nor endorsed by FMS. It is expressly declared that this e-mail does not constitute nor form part of any contract or unilateral obligation unless the contrary is specifically stated in this e-mail and authorised by FMS. From Karen.Heckford at DignityHealth.org Thu May 21 11:57:16 2015 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Thu, 21 May 2015 09:57:16 -0700 Subject: [Histonet] Formalin waste Message-ID: I was just wondering the Labs in the San Francisco Bay Area. How are you getting rid of your formalin waste? Are you Neutralizing your formalin like with Neutralex or having it picked up and taken off site? Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From Kimberly at animalreferencepathology.com Thu May 21 12:25:04 2015 From: Kimberly at animalreferencepathology.com (Kimberly Marshall) Date: Thu, 21 May 2015 17:25:04 +0000 Subject: [Histonet] Microwave Decalcification Message-ID: <1432229164867.39451@animalreferencepathology.com> ?Can anyone that has used the microwave decalcification method, please let me know how it worked for you? Thanks in advance. Kimberly Kimberly Marshall H.T.(ASCP) Histology/Lab Supervisor Toll Free 1-800-426-2099 Fax 801-584-5104 PO Box 17580 Salt Lake City, Utah 84107 www.animalreferencepathology.com Advancing the art and science of veterinary medicine [cid:image001.jpg at 01CF8F87.A0BD4830] From hmarlatt26 at gmail.com Thu May 21 14:49:48 2015 From: hmarlatt26 at gmail.com (Heather Marlatt) Date: Thu, 21 May 2015 12:49:48 -0700 Subject: [Histonet] Donating Old Microtome Message-ID: I'm posting on behalf of a local researcher. They have been using an older microtome that was previously donated by a hospital. It broke down recently and they cant afford to replace it right now. It is used by the students to cut their own sections so they just need something that can section at 4 microns. They are happy to pay the freight if anyone out there has an old working microtome that they are willing to donate. Thank you!!! Heather From ltougas at dawsoncollege.qc.ca Thu May 21 22:24:09 2015 From: ltougas at dawsoncollege.qc.ca (Liette Tougas) Date: Fri, 22 May 2015 03:24:09 +0000 Subject: [Histonet] Donating Old Microtome In-Reply-To: References: Message-ID: <455897B94CF3A44F81F727160F23FB11640C5799@DC229.ad.dawsoncollege.qc.ca> Hi Heather, I do not know where you are located but we have 5-6 good old AO microtomes that still work very well that we are willing to donate. They are in excellent condition as they were extremely well maintained over the years and, as a Biomed Lab technology teaching department, they have been used only twice a week for 3 weeks each fall semester since they were purchased, some 30 years ago! They each have a disposable blade holder (high profile) and block clamp (vs original screwing block holder). We easily cut at 4 microns on them. The offer is opened to anyone who can pick them up or pay for shipment. Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College, Montr?al, Qc, Canada 514-931-8731, ext 1519 ________________________________________ From: Heather Marlatt [hmarlatt26 at gmail.com] Sent: May 21, 2015 3:49 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Donating Old Microtome I'm posting on behalf of a local researcher. They have been using an older microtome that was previously donated by a hospital. It broke down recently and they cant afford to replace it right now. It is used by the students to cut their own sections so they just need something that can section at 4 microns. They are happy to pay the freight if anyone out there has an old working microtome that they are willing to donate. Thank you!!! Heather _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan at uwo.ca Fri May 22 00:11:08 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Fri, 22 May 2015 00:11:08 -0500 Subject: [Histonet] Donating Old Microtome In-Reply-To: <73e0c6ec858e.555eba50@uwo.ca> References: <455897B94CF3A44F81F727160F23FB11640C5799@DC229.ad.dawsoncollege.qc.ca> <7380c203f3e9.555eb8df@uwo.ca> <7300a33db1ab.555eb958@uwo.ca> <7320966bfcc1.555eb996@uwo.ca> <7340902ed903.555eb9d4@uwo.ca> <7340e93bb18b.555eba12@uwo.ca> <73e0c6ec858e.555eba50@uwo.ca> Message-ID: <7270cea3faf9.555e741c@uwo.ca> Why get rid of microtomes that still work well? Please explain. John Kiernan London, Canada = = = On 21/05/15, Liette Tougas wrote: > Hi Heather, > > I do not know where you are located but we have 5-6 good old AO microtomes that still work very well that we are willing to donate. They are in excellent condition as they were extremely well maintained over the years and, as a Biomed Lab technology teaching department, they have been used only twice a week for 3 weeks each fall semester since they were purchased, some 30 years ago! They each have a disposable blade holder (high profile) and block clamp (vs original screwing block holder). We easily cut at 4 microns on them. The offer is opened to anyone who can pick them up or pay for shipment. > > Liette Tougas, RT, B.Sc., M.Sc. > Biomedical Laboratory Technology Department > Dawson College, Montr?al, Qc, Canada > 514-931-8731, ext 1519 > ________________________________________ > From: Heather Marlatt [hmarlatt26 at gmail.com] > Sent: May 21, 2015 3:49 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Donating Old Microtome > > I'm posting on behalf of a local researcher. They have been using an older > microtome that was previously donated by a hospital. It broke down recently > and they cant afford to replace it right now. It is used by the students to > cut their own sections so they just need something that can section at 4 > microns. They are happy to pay the freight if anyone out there has an old > working microtome that they are willing to donate. > > Thank you!!! > > Heather > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From cjohnson at nmda.nmsu.edu Fri May 22 11:17:02 2015 From: cjohnson at nmda.nmsu.edu (Johnson, Carole) Date: Fri, 22 May 2015 16:17:02 +0000 Subject: [Histonet] Buying service contracts Message-ID: Hello and Happy Friday everyone! So I am looking at purchase of a cassette and slide printer and would like to know what you think about the value in purchasing additional year service contracts? Thanks! Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From Timothy.Morken at ucsf.edu Fri May 22 11:25:58 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 22 May 2015 16:25:58 +0000 Subject: [Histonet] Buying service contracts In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF3683489A@ex07.net.ucsf.edu> Carole, I determine that by how critical it is to have it working, how long you can go without, whether I have backup units and how long it takes to get non-priority one-off repair service if something goes down. We do have contracts on most of our equipment. If it is a common item that a third-party service vendor can handle you can often get a contract cheaper, or get faster one-off repair service faster than you can through the original manufacturer service group. For instance, we cover our microtomes with a third part vendor because the annual service contract is way too expensive. They rarely go down and we have plenty of backup units due to our shift schedules. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Johnson, Carole [mailto:cjohnson at nmda.nmsu.edu] Sent: Friday, May 22, 2015 9:17 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Buying service contracts Hello and Happy Friday everyone! So I am looking at purchase of a cassette and slide printer and would like to know what you think about the value in purchasing additional year service contracts? Thanks! Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Fri May 22 12:06:56 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 22 May 2015 17:06:56 +0000 Subject: [Histonet] old microtomes that work In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F8013474@HRHEX02-HOS.holyredeemer.local> We got rid of Microtomes that still work because we went to a more ergonomically friendly model. Just because you can get a decent section off of a microtome, doesn't mean that you would want to cut 300+ blocks a day on it! Have a safe and peaceful Memorial Day weekend. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 4. Re: Donating Old Microtome (John Kiernan) From: John Kiernan Why get rid of microtomes that still work well? Please explain. John Kiernan London, Canada From thomas.huynh at mdanderson.org Fri May 22 12:39:26 2015 From: thomas.huynh at mdanderson.org (Huynh,Thomas) Date: Fri, 22 May 2015 17:39:26 +0000 Subject: [Histonet] Microwave Decalcification Message-ID: Hi Kimberly, We are currently using the Milestone Medical brand microwave to decalcify our bone marrow core biopsies. After fixation we decal the core biopsies in the microwave for 2.5 hours at 50 degree Celsius with the 10% Formic Acid (we made up the formic acid in-house). If you need more info please email me. Thanks Thomas -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Friday, May 22, 2015 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 138, Issue 26 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Microwave Decalcification (Kimberly Marshall) 2. Donating Old Microtome (Heather Marlatt) 3. Re: Donating Old Microtome (Liette Tougas) 4. Re: Donating Old Microtome (John Kiernan) 5. Buying service contracts (Johnson, Carole) 6. Re: Buying service contracts (Morken, Timothy) ---------------------------------------------------------------------- Message: 1 Date: Thu, 21 May 2015 17:25:04 +0000 From: Kimberly Marshall To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Microwave Decalcification Message-ID: <1432229164867.39451 at animalreferencepathology.com> Content-Type: text/plain; charset="iso-8859-1" ?Can anyone that has used the microwave decalcification method, please let me know how it worked for you? Thanks in advance. Kimberly Kimberly Marshall H.T.(ASCP) Histology/Lab Supervisor Toll Free 1-800-426-2099 Fax 801-584-5104 PO Box 17580 Salt Lake City, Utah 84107 www.animalreferencepathology.com Advancing the art and science of veterinary medicine [cid:image001.jpg at 01CF8F87.A0BD4830] ------------------------------ Message: 2 Date: Thu, 21 May 2015 12:49:48 -0700 From: Heather Marlatt To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Donating Old Microtome Message-ID: Content-Type: text/plain; charset=UTF-8 I'm posting on behalf of a local researcher. They have been using an older microtome that was previously donated by a hospital. It broke down recently and they cant afford to replace it right now. It is used by the students to cut their own sections so they just need something that can section at 4 microns. They are happy to pay the freight if anyone out there has an old working microtome that they are willing to donate. Thank you!!! Heather ------------------------------ Message: 3 Date: Fri, 22 May 2015 03:24:09 +0000 From: Liette Tougas To: Heather Marlatt , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Donating Old Microtome Message-ID: <455897B94CF3A44F81F727160F23FB11640C5799 at DC229.ad.dawsoncollege.qc.ca> Content-Type: text/plain; charset="iso-8859-1" Hi Heather, I do not know where you are located but we have 5-6 good old AO microtomes that still work very well that we are willing to donate. They are in excellent condition as they were extremely well maintained over the years and, as a Biomed Lab technology teaching department, they have been used only twice a week for 3 weeks each fall semester since they were purchased, some 30 years ago! They each have a disposable blade holder (high profile) and block clamp (vs original screwing block holder). We easily cut at 4 microns on them. The offer is opened to anyone who can pick them up or pay for shipment. Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College, Montr?al, Qc, Canada 514-931-8731, ext 1519 ________________________________________ From: Heather Marlatt [hmarlatt26 at gmail.com] Sent: May 21, 2015 3:49 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Donating Old Microtome I'm posting on behalf of a local researcher. They have been using an older microtome that was previously donated by a hospital. It broke down recently and they cant afford to replace it right now. It is used by the students to cut their own sections so they just need something that can section at 4 microns. They are happy to pay the freight if anyone out there has an old working microtome that they are willing to donate. Thank you!!! Heather _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 22 May 2015 00:11:08 -0500 From: John Kiernan To: Liette Tougas , Heather Marlatt , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Donating Old Microtome Message-ID: <7270cea3faf9.555e741c at uwo.ca> Content-Type: text/plain; charset=iso-8859-1 Why get rid of microtomes that still work well? Please explain. John Kiernan London, Canada = = = On 21/05/15, Liette Tougas wrote: > Hi Heather, > > I do not know where you are located but we have 5-6 good old AO microtomes that still work very well that we are willing to donate. They are in excellent condition as they were extremely well maintained over the years and, as a Biomed Lab technology teaching department, they have been used only twice a week for 3 weeks each fall semester since they were purchased, some 30 years ago! They each have a disposable blade holder (high profile) and block clamp (vs original screwing block holder). We easily cut at 4 microns on them. The offer is opened to anyone who can pick them up or pay for shipment. > > Liette Tougas, RT, B.Sc., M.Sc. > Biomedical Laboratory Technology Department Dawson College, Montr?al, > Qc, Canada 514-931-8731, ext 1519 > ________________________________________ > From: Heather Marlatt [hmarlatt26 at gmail.com] > Sent: May 21, 2015 3:49 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Donating Old Microtome > > I'm posting on behalf of a local researcher. They have been using an > older microtome that was previously donated by a hospital. It broke > down recently and they cant afford to replace it right now. It is used > by the students to cut their own sections so they just need something > that can section at 4 microns. They are happy to pay the freight if > anyone out there has an old working microtome that they are willing to donate. > > Thank you!!! > > Heather > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 5 Date: Fri, 22 May 2015 16:17:02 +0000 From: "Johnson, Carole" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Buying service contracts Message-ID: Content-Type: text/plain; charset="us-ascii" Hello and Happy Friday everyone! So I am looking at purchase of a cassette and slide printer and would like to know what you think about the value in purchasing additional year service contracts? Thanks! Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. ------------------------------ Message: 6 Date: Fri, 22 May 2015 16:25:58 +0000 From: "Morken, Timothy" To: "Johnson, Carole" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Buying service contracts Message-ID: <761E2B5697F795489C8710BCC72141FF3683489A at ex07.net.ucsf.edu> Content-Type: text/plain; charset="us-ascii" Carole, I determine that by how critical it is to have it working, how long you can go without, whether I have backup units and how long it takes to get non-priority one-off repair service if something goes down. We do have contracts on most of our equipment. If it is a common item that a third-party service vendor can handle you can often get a contract cheaper, or get faster one-off repair service faster than you can through the original manufacturer service group. For instance, we cover our microtomes with a third part vendor because the annual service contract is way too expensive. They rarely go down and we have plenty of backup units due to our shift schedules. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Johnson, Carole [mailto:cjohnson at nmda.nmsu.edu] Sent: Friday, May 22, 2015 9:17 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Buying service contracts Hello and Happy Friday everyone! So I am looking at purchase of a cassette and slide printer and would like to know what you think about the value in purchasing additional year service contracts? Thanks! Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 138, Issue 26 ***************************************** From Timothy.Morken at ucsf.edu Fri May 22 12:51:28 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 22 May 2015 17:51:28 +0000 Subject: [Histonet] Pathologist' assistant, two openings, UCSF Message-ID: <761E2B5697F795489C8710BCC72141FF36834934@ex07.net.ucsf.edu> UC San Francisco has two Pathologist Assistant openings due to expansion of the service. Certified, experienced preferred, but recent graduates are encouraged to apply One opening is for the Parnassus Campus at Moffitt Hospital (near Golden Gate Park). The other is for the new Mission Bay Hospital Campus near SF Bay. Apply online at: http://jobs.ucsfmedicalcenter.org/ Search for "Pathologist assistant" Job posting ID's are 8522 and 8374 Job Description Job ID: 8522 Job Title: Pathologists' Assistant - Pathology-Morgue (SPEC) Job Code: 0461 Department: Pathology-Morgue Location: Mission Bay Full/Part Time: Full-Time Appointment Type: Career Shift: 8-hour Variable Weekly Hours: 40 ; 100% Union Information: This classification is not represented by a union ________________________________ Return to Previous Page ________________________________ Our legacy of unsurpassed patient care and unceasing mission to integrate high-tech medical research with clinical operations has led to our prestigious standing as one of the top 10 hospitals in the nation according to U.S. News & World Report. As a premier health care institution dedicated to advancing health worldwide, UCSF Medical Center can also be the best place to advance and shape your career. The medical center's employees are one of the most important reasons why we are recognized as one of the nation's best hospitals. To work at UCSF Medical Center is to be part of an institution that provides the highest caliber of care to patients; a nurturing, dynamic and team-oriented atmosphere in which to best use your skills and talents. Job Summary Under the direction of the directors, serves as a technical expert in UCSF Surgical Pathology Gross Rooms, providing technical assistance and direction to trainees and pathologists' assistants (PAs). The primary role of the incumbent is practitioner, educator and role model for trainees and staff members in grossing the most complex cancer specimens (pancreas, breast, bladder, uterus, ovary, etc.) as well as simple benign resections and biopsies. It is the role of the PA to continuously interact with the laboratory director(s), site managers, supervisors and designees. The incumbent determines the need to consult with a pathologist, makes technical grossing decisions, troubleshoots and resolves specimen receiving issues. The incumbent must have the basic knowledge of the full scope of general PA technical duties in the laboratory when required (lab preparation, receiving, accessioning, performing an intraoperative evaluation including cryostat frozen sectioning and staining, gross sectioning, dictation, photography, etc.). The incumbent must also have a thorough understanding of what and why these sections are essential, and perform duties efficiently (focusing on quality and quantity). As determined, works variable schedules and rotates at other work sites as needed. Provides technical support to the Autopsy Service and Histology Laboratory as needed. Other duties as assigned . The flexibility to orient and work at all UCSF Medical Center locations is required. LIVING PRIDE STANDARDS Service Excellence * Demonstrates service excellence by following the Everyday PRIDE Guide with the UCSF Medical Center standards and expectations for communication and behavior. These standards and expectations convey specific behavior associated with the Medical Center's values: Professionalism, Respect, Integrity, Diversity and Excellence, and provide guidance on how we communicate with patients, visitors, faculty, staff, and students, virtually everyone, every day and with every encounter. These standards include, but are not limited to: personal appearance, acknowledging and greeting all patients and families, introductions using AIDET, managing up, service recovery, managing delays and expectations, phone standards, electronic communication, team work, cultural sensitivity and competency. * Uses effective communication skills with patients and staff; demonstrates proper telephone techniques and etiquette; acts as an escort to any patient or family member needing directions; shows sensitivity to differences of culture; demonstrates a positive and supportive manner in which patients / families/ colleagues perceive interactions as positive and supportive. Exhibits team work skills to positively acknowledge and recognize other colleagues, and uses personal experiences to model and teach Living PRIDE standards. * Exhibits tact and professionalism in difficult situations according to PRIDE Values and Practices * Demonstrates an understanding of and adheres to privacy, confidentiality, and security policies and procedures related to Protected Health Information (PHI) or other sensitive and personal information. * Demonstrates an understanding of and adheres to safety and infection control policies and procedures. * Assumes accountability for improving quality metrics associated with department/unit and meeting organizational/departmental targets. Work Environment * Keeps working areas neat, orderly and clutter-free, including the hallways. Adheres to cleaning processes and puts things back where they belong. Removes and reports broken equipment and furniture. * Picks up and disposes of any litter found throughout entire facility. * Posts flyers and posters in designated areas only; does not post on walls, doors or windows. * Knows where the Environment of Care Manual is kept in department; corrects or reports unsafe conditions to the appropriate departments. * Protects the physical environment and equipment from damage and theft. Required Qualifications * A graduate from a National Accrediting Agency for Clinical Laboratory Sciences (NAACLS) accredited pathologists' assistant training program * Bachelor's Degree * ASCP certified or certification-eligible as pathologists' assistant * Certification-eligible candidates must pass the certification examination within one year of hire * Once achieved, American Society for Clinical Pathology (ASCP) certification must be maintained as long as one works in this position * Demonstrated proficiency in teaching, communication and interpersonal skills * Flexibility to orient and work in all UCSF Medical Center locations as needed * Knowledge of medical terminology, specifically understanding of Pathology * Ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines * Excellent data entry, word processing, spelling, grammar, and editing skills * The flexibility to orient and work at all UCSF Medical Center locations Preferred Qualifications * Master's Degree * Current Fellow member of the American Association of Pathologists' Assistants (AAPA) * Current Member of the American Society for Clinical Pathology * Previous leadership experience in a hospital laboratory * Experience working in the Pathology Department of an academic health center Licensure/Certification * ASCP certified or certification-eligible as pathologists' assistant * Certification-eligible candidates must pass the certification examination within one year of hire * Once achieved, American Society for Clinical Pathology (ASCP) certification must be maintained as long as one works in this position Equal Employment Opportunity The University of California San Francisco is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, or protected veteran status. Further information about the University of California, San Francisco, is available at diversity.ucsf.edu. UCSF seeks candidates whose skills, and personal and professional experience, have prepared them to contribute to our commitment to diversity and excellence, and the communities we serve. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken at ucsfmedctr.org From PAMarcum at uams.edu Fri May 22 13:20:21 2015 From: PAMarcum at uams.edu (Marcum, Pamela A) Date: Fri, 22 May 2015 18:20:21 +0000 Subject: [Histonet] HT/HTL PRN Part-time to permanent Part-time or Full Time Message-ID: We have one part-time PRN positions open at UAMS in Little Rock AR and a full-time Histology position. The person must be ASCP registered as either HT or HTL to qualify. We are a very automated lab for special stains and routine staining, including coverslippers. Computer skills will be needed as we are working with Epic Beaker for EMR. The University of Arkansas for Medical Sciences had excellent benefits for employees. We are not offering move assistance or bonuses at this time and I feel it is only fair to state that if you are interested and do not live in the area. Please no recruiters as we are a state institution and are not allowed to use your services. Thank You, Pam Marcum ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From ltougas at dawsoncollege.qc.ca Sun May 24 06:44:24 2015 From: ltougas at dawsoncollege.qc.ca (Liette Tougas) Date: Sun, 24 May 2015 11:44:24 +0000 Subject: [Histonet] Donating Old Microtome In-Reply-To: <7270cea3faf9.555e741c@uwo.ca> References: <455897B94CF3A44F81F727160F23FB11640C5799@DC229.ad.dawsoncollege.qc.ca> <7380c203f3e9.555eb8df@uwo.ca> <7300a33db1ab.555eb958@uwo.ca> <7320966bfcc1.555eb996@uwo.ca> <7340902ed903.555eb9d4@uwo.ca> <7340e93bb18b.555eba12@uwo.ca> <73e0c6ec858e.555eba50@uwo.ca>,<7270cea3faf9.555e741c@uwo.ca> Message-ID: <455897B94CF3A44F81F727160F23FB11640C5998@DC229.ad.dawsoncollege.qc.ca> Hi Mr. Kieran, I know, I am sad to see them go as well since they are in such good condition but, as a med lab teaching program, not having knife/blade gards (we were using cut pieces of rubber tubing at each end of the holder which would easily fall off!) has become a safety issue for us. Best regards, Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College 514-931-8731, ext 1519 ________________________________________ From: John Kiernan [jkiernan at uwo.ca] Sent: May 22, 2015 1:11 AM To: Liette Tougas; Heather Marlatt; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Donating Old Microtome Why get rid of microtomes that still work well? Please explain. John Kiernan London, Canada = = = On 21/05/15, Liette Tougas wrote: Hi Heather, I do not know where you are located but we have 5-6 good old AO microtomes that still work very well that we are willing to donate. They are in excellent condition as they were extremely well maintained over the years and, as a Biomed Lab technology teaching department, they have been used only twice a week for 3 weeks each fall semester since they were purchased, some 30 years ago! They each have a disposable blade holder (high profile) and block clamp (vs original screwing block holder). We easily cut at 4 microns on them. The offer is opened to anyone who can pick them up or pay for shipment. Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College, Montr?al, Qc, Canada 514-931-8731, ext 1519 ________________________________________ From: Heather Marlatt [hmarlatt26 at gmail.com] Sent: May 21, 2015 3:49 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Donating Old Microtome I'm posting on behalf of a local researcher. They have been using an older microtome that was previously donated by a hospital. It broke down recently and they cant afford to replace it right now. It is used by the students to cut their own sections so they just need something that can section at 4 microns. They are happy to pay the freight if anyone out there has an old working microtome that they are willing to donate. Thank you!!! Heather _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garreyf at gmail.com Sun May 24 11:13:58 2015 From: garreyf at gmail.com (Garrey Faller) Date: Sun, 24 May 2015 12:13:58 -0400 Subject: [Histonet] Waterbath cleanliness Message-ID: Hi everyone, I am looking for a histotech procedure for keeping the water bath clean and for preventing cross contamination of patient specimens. I know one should use a kim wipe (or something similar) to clean the water surface of any ribbon debris between cases. However, should the top edge of the water bath be clean of all debris between every case? Sometimes I see a mound-like build up of paraffin ribbon debris, and I am told that this is a normal practice while others say that it is not. I'd like to modify my policy to say that there should be no debris on the water surface or on the hard top edge of the water bath in between cases. Is this out of line? Is this what everyone practices? Also, with the exception of rush type cases and perhaps large specimens, does everyone embed in numerical order and then cut in numerical order? Thanks in advance for your help. Garrey Faller Pathologist From suetp918 at comcast.net Sun May 24 20:02:05 2015 From: suetp918 at comcast.net (Susan Paturzo) Date: Sun, 24 May 2015 21:02:05 -0400 Subject: [Histonet] Waterbath cleanliness In-Reply-To: References: Message-ID: <79DD4BFB-F6CF-4A4F-9A40-BCD246F2C59D@comcast.net> We do clean between every block. As 4 adding water during cutting We clean once that water has reached temp. Sometimes there is paraffin debris on the edge of bath due to tagging ribbon. If we cut a cyst or fatty tissue we will completely change the water Sent from my iPad > On May 24, 2015, at 12:13 PM, Garrey Faller wrote: > > Hi everyone, > > I am looking for a histotech procedure for keeping the water bath clean and > for preventing cross contamination of patient specimens. I know one should > use a kim wipe (or something similar) to clean the water surface of any > ribbon debris between cases. However, should the top edge of the water bath > be clean of all debris between every case? Sometimes I see a mound-like > build up of paraffin ribbon debris, and I am told that this is a normal > practice while others say that it is not. > I'd like to modify my policy to say that there should be no debris on the > water surface or on the hard top edge of the water bath in between cases. > Is this out of line? Is this what everyone practices? > > Also, with the exception of rush type cases and perhaps large specimens, > does everyone embed in numerical order and then cut in numerical order? > > Thanks in advance for your help. > > Garrey Faller > Pathologist > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 at comcast.net Sun May 24 20:03:03 2015 From: suetp918 at comcast.net (Susan Paturzo) Date: Sun, 24 May 2015 21:03:03 -0400 Subject: [Histonet] Waterbath cleanliness In-Reply-To: References: Message-ID: <6E259E41-634F-4EBC-A2D6-CB24C76CE1AA@comcast.net> We do not embed in order since we have 6 grossers but we do cut in order Sent from my iPad > On May 24, 2015, at 12:13 PM, Garrey Faller wrote: > > Hi everyone, > > I am looking for a histotech procedure for keeping the water bath clean and > for preventing cross contamination of patient specimens. I know one should > use a kim wipe (or something similar) to clean the water surface of any > ribbon debris between cases. However, should the top edge of the water bath > be clean of all debris between every case? Sometimes I see a mound-like > build up of paraffin ribbon debris, and I am told that this is a normal > practice while others say that it is not. > I'd like to modify my policy to say that there should be no debris on the > water surface or on the hard top edge of the water bath in between cases. > Is this out of line? Is this what everyone practices? > > Also, with the exception of rush type cases and perhaps large specimens, > does everyone embed in numerical order and then cut in numerical order? > > Thanks in advance for your help. > > Garrey Faller > Pathologist > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 at mail.ru Mon May 25 12:56:07 2015 From: Maxim_71 at mail.ru (Maxim Peshkov) Date: Mon, 25 May 2015 20:56:07 +0300 Subject: [Histonet] Waterbath cleanliness Message-ID: <835739586.20150525205607@mail.ru> Garrey, We add to water bath 3-4 drops of Tween 20. It is decrease the surface tension and small debrises of wax and tissues. Also sections better become flat. Adding of Tween 20 is necessary for all type of sections: for HE, HC, IHC. Sincerely, Maxim Peshkov, Russia, Taganrog. mailto:Maxim_71 at mail.ru From ruio7 at hotmail.com Tue May 26 01:59:11 2015 From: ruio7 at hotmail.com (Rui TAHARA) Date: Tue, 26 May 2015 15:59:11 +0900 Subject: [Histonet] dehydration time for a relatively large sample In-Reply-To: <8A886B98D8F46141B553250B4B5A1343C4F30A5F@SVSLS103V223.regsj.intern> References: , , <8A886B98D8F46141B553250B4B5A1343C4F19645@SVSLS103V223.regsj.intern>, , <8A886B98D8F46141B553250B4B5A1343C4F30A5F@SVSLS103V223.regsj.intern> Message-ID: Thank you for your advice! I was going to try if the current sample that was embedded by a technician at the histological service center. This is the first time that i asked for an expert to prepare my sample. Then I sectioned the sample by myself. However, the muscles in the section always looks very dry or are unable to be penetrated with wax, thus the sections do not look that great.... I am not an expert at all for preparing histological sections, so if the sample embedded by an expert, I am not sure how to fix the problem. I may try it again following your time schedule, but i am a bit tight schedule to end this project. If i try i will let you know how it turns out. I currently reside in montreal, Canada and am studying as a PhD student. I really appreciate your help. rui > From: krns at regionsjaelland.dk > To: ruio7 at hotmail.com > Subject: SV: [Histonet] dehydration time for a relatively large sample > Date: Thu, 21 May 2015 13:24:56 +0000 > > Hi Rui > > Sorry for a little late response. I tried to write to you yesterday but my internet failed. > > Tissue is so much easier with a processor, but life is not always easy and we have to do things the possible way ;-) > > First I have to tell you, that I never have tried to process tissue by hand..... and I have never tried to process zebra finch. BUT.... I have been helping lot of other people with protocols, so I think I'm able to help you too. maybe we need to adjust the protocol. ... But lets see how it will work. > > First.... make sure your tissue are well fixed before decalcination. 1 day in formalin. > Decalcinate the tissue as short time as possible but make sure it's with out calcium before processing. > > 70% ethanol 3 hours > 96 % ethanol 5-7 hours > 99 % over night ( about 12 hours ) > 99 % so it will fit your work ( change 99% 2 times) > Clearens over night (about 12 hours ) > Clearens so it will fit your work ( change clearens 2 times ) > Paraffin 2-3 days ( change paraffin 3 times ) > > When you embed the tissue please notise if you see lot of small bobles. The bobles can be a sign of poorly infiltretet paraffin (water in the tissue) > > Please let my know how things go, because we maybe need some adjustment. > > I would like to know where you are from. I have helped all over the would and it's fun to know. > > Kind regards > Karen > it's___________________________ > Fra: Rui TAHARA [ruio7 at hotmail.com] > Sendt: 20. maj 2015 15:44 > Til: histonet at lists.utsouthwestern.edu > Emne: Re: [Histonet] dehydration time for a relatively large sample > > Hi, > > Thanks for prompt response. > We unfortunately do not have a processor in our lab at university..The protocol i wrote was working in quail embryonic samples (just before hatching). I process the tissue manually and cannot process the tissue with strict time schedule. Thus, I need to leave a sample overnight at some point. > > I cut the zebra finch head into anterior (beak) and posterior (brain) region and mid-sagittally in both. so each tissue sample is about 0.5 X 1 X 1cm cube at maximum. > > I have also tried short time schedule compared the one i wrote in previous email, for similar sized sample (zebra finch beak). However, it never worked. Thus i prolonged the each step for the latest sample. > It would be great if you could provide me the proper time schedule. > > rui > > > From: krns at regionsjaelland.dk > > To: ruio7 at hotmail.com > > Subject: SV: [Histonet] dehydration time for a relatively large sample > > Date: Wed, 20 May 2015 06:18:24 +0000 > > > > Hi Rui > > > > Which processor unit do you use > > > > to me it seems like a wrong protocol. > > > > Maybe I can help you set up a better protocol - if you want - but then I need to know size og your tissue, processor unit and what kind of clearens you use. > > > > Kind regards > > Karen > > Supervisor tissue processing > > Denmark > > > > -----Oprindelig meddelelse----- > > Fra: Rui TAHARA [mailto:ruio7 at hotmail.com] > > Sendt: Wednesday, May 20, 2015 7:11 AM > > Til: histonet at lists.utsouthwestern.edu > > Emne: [Histonet] dehydration time for a relatively large sample > > > > Hi, > > > > I am wondering if prolong dehydration time with 95 and 100% ethanol would brittle the sample for paraffin sectioning. > > > > I have been trying to section adult zebra finch beak and processed several samples, however, > > i failed to obtain a good section. It appears that the paraffin did not penetrated the tissue. This may be derived from incomplete dehydration and clearing before paraffin. > > Because i have read somewhere that if the sample was sitting in 95 and 100% ethanol too long it would be brittle and be teared when sectioned. I have processed only a beak (about 1 cmX0.5 mm). > > Also when is an appropriate time to use vacuum? I am afraid that if i used it at 100% ethanol, the ethanol would evaporate.... > > > > so far I have tried; > > fix overnight > > decalcified few days > > walk from water to 70% ethanol; overnight > > 85%, 95% (2times change) and 100 % (2 times change) ethanol; overnight > > clearing; 2 days > > paraffin (2 times change); overnight > > > > Any suggestion would be appreciated. > > Thank you in advance, > > > > rui > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.benavides at eae.csic.es Tue May 26 04:17:44 2015 From: j.benavides at eae.csic.es (Julio Benavides) Date: Tue, 26 May 2015 11:17:44 +0200 Subject: [Histonet] Crystallized formalin In-Reply-To: References: Message-ID: <55643A38.3030804@eae.csic.es> Hi there, we have a bottle of formaline 40% with crystals at the bottom. Can we used it? is there any way to re-suspend the crystals? Better dispose of it? and, BTW, do you know why it crystallized? by its side there is another bottle, same batch, with no crystals. thanks a lot your comments Regards Julio From jqb7 at cdc.gov Tue May 26 06:08:05 2015 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue, 26 May 2015 11:08:05 +0000 Subject: [Histonet] Waterbath cleanliness In-Reply-To: References: Message-ID: <3B2CD438E1628A41BD687E98B963B78137F3EE0E@EMBX-CLFT4.cdc.gov> Hi, Paraffin build-up should not be a common practice. I recall being told as a student decades ago, "The waterbath should be kept scrupulously clean". Some things are still true today and this is one of them. Does your waterbath have a glass insert? If so, simply wipe off all of the paraffin you can and then wipe off remaining debris with a small amount of xylene and wipe that off with alcohol. Then a quick wash in warm soapy water takes care of the rest. In a high-volume lab a quick wash in the lab dishwasher is perfect. My waterbath has no insert...just a solid black finish and of course it cannot be submerged in water and xylene is out of the question. I just make sure there is no paraffin on the edges and if some happens to get stuck there in spite of my efforts then I use a small amount of alcohol on gauze and remove it. Then I rinse the waterbath in distilled water, dry it out and then squirt a small amount of alcohol in it, swish it around and wipe it dry. We also only use distilled water in our waterbath. Jeanine Sanders CDC Atlanta -----Original Message----- From: Garrey Faller [mailto:garreyf at gmail.com] Sent: Sunday, May 24, 2015 12:14 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Waterbath cleanliness Hi everyone, I am looking for a histotech procedure for keeping the water bath clean and for preventing cross contamination of patient specimens. I know one should use a kim wipe (or something similar) to clean the water surface of any ribbon debris between cases. However, should the top edge of the water bath be clean of all debris between every case? Sometimes I see a mound-like build up of paraffin ribbon debris, and I am told that this is a normal practice while others say that it is not. I'd like to modify my policy to say that there should be no debris on the water surface or on the hard top edge of the water bath in between cases. Is this out of line? Is this what everyone practices? Also, with the exception of rush type cases and perhaps large specimens, does everyone embed in numerical order and then cut in numerical order? Thanks in advance for your help. Garrey Faller Pathologist _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz at premierlab.com Tue May 26 12:25:04 2015 From: liz at premierlab.com (Elizabeth Chlipala) Date: Tue, 26 May 2015 11:25:04 -0600 Subject: [Histonet] NSH Awards Message-ID: <14E2C6176416974295479C64A11CB9AE02230F9D8D68@SBS2K8.premierlab.local> Hello Everyone It's getting really close to June 1st which is the deadline for nominations for NSH Awards. I know that you have heard me say over and over again how important it is to nominate yourself of other individuals for awards but I am going to keep doing it. So here it goes....... There are three categories for awards; leadership, education and advocacy. These awards offer financial incentives for continuing education along with recognizing the individuals within our profession who have demonstrated both dedication and excellence in the field of Histotechnology. I can't stress to you enough how great it is that the NSH can provide this much support to their members for continuing education opportunities. These opportunities are provided through the generosity of the sponsors, if it were not for them we would not have these awards. It's also so important that we take time out of our busy days to nominate individuals in our field that are raising the bar and setting a higher standard, these individuals need and should be recognized and what a better way to do that then to nominate them for an award. I challenge all of you to take the time to nominate one individual you feel is deserving of one of the many awards the NSH has to offer. We have two new awards this year. The Frank J. Monek Memorial Scholarship, this generous award of $10,000 is sponsored by Cancer Diagnostics along with Andrea, Frank and Michelle Monek and the The Peggy Wenk Histology Program Award and Scholarship. Sakura Finetek will award $5,000 to a HT or HTL NAACLS approved program in honor of Peggy Wenk. There are so many other awards, too numerous to list here, so to see the list of awards available head to the NSH website http://nsh.org/scholarships-awards. Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From BDeBrosse-Serra at isisph.com Tue May 26 13:09:10 2015 From: BDeBrosse-Serra at isisph.com (Bea DeBrosse-Serra) Date: Tue, 26 May 2015 18:09:10 +0000 Subject: [Histonet] Who are you guys using to service equipment in the San Diego area? Message-ID: Who are you guys using for servicing different equipment like microtome, cryostat, stainers in the San Diego area? Thank you in advance, Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 From jkiernan at uwo.ca Tue May 26 22:41:09 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Tue, 26 May 2015 22:41:09 -0500 Subject: [Histonet] Crystallized formalin In-Reply-To: <73b0c10d6a498.55653cbf@uwo.ca> References: <55643A38.3030804@eae.csic.es> <73e0b33f36dd1.5564b2bc@uwo.ca> <7330f4c831e2a.5564b2fa@uwo.ca> <73c0e3c7361c3.5564b339@uwo.ca> <7320b5373754f.5564b4a4@uwo.ca> <71e0e86f30cb1.5564b4e4@uwo.ca> <7350e34f35be6.5564c064@uwo.ca> <73208803358a5.5564c701@uwo.ca> <7260d8c36a8a9.556535f2@uwo.ca> <73408d0468dc9.55653631@uwo.ca> <7260907d6ae0d.556536ac@uwo.ca> <7340a1c86f9de.556536eb@uwo.ca> <73e0e77068e1e.55653765@uwo.ca> <7260d35a6e8c9.556537a3@uwo.ca> <72d0b72e6d748.556537e1@uwo.ca> <72d0e0316853d.55653820@uwo.ca> <73e0887e6bc60.556538d7@uwo.ca> <7260ac026dada.55653915@uwo.ca> <726093726e16d.55653954@uwo.ca> <7330a7d96caa0.55653992@uwo.ca> <7240b82f6f53d.556539d1@uwo.ca> <72d0d55b6c900.55653a4c@uwo.ca> <72f0e7e76a6e8.55653a8b@uwo.ca> <73e0aecf6e3ae.55653ac9@uwo.ca> <7260b55a6af97.55653b07@uwo.ca> <7220a32e6d1cc.55653b47@uwo.ca> <7220a24a6ff1b.55653b86@uwo.ca> <7240919d68fdf.55653bc5@uwo.ca> <7240b4c268e2f.55653c04@uwo.ca> <72a087d468623.55653c42@uwo.ca> <72d0ee516f300.55653c80@uwo.ca> <73b0c10d6a498.55653cbf@uwo.ca> Message-ID: <72d0d0236978c.5564f685@uwo.ca> The formaldehyde in formalin is present as low polymers. The depolymerize when the liquid is diluted. With storage, especially at lower temperatures, the small polymers join together anf form large insoluble polymers, known as paraformaldehyde. This reduces the effective concentration of formaldehyde in the fixative solution. (At a guess, it might be down from 4% to 3%. ). This probably doesn't matter. Read about formalin and formaldehyde at Microscopy Today 08-01: 8-12 (2000). This is a free download from Microscopy Today's web site, and it includes references to more significant publications. Paraformaldehyde is easily depolymerized. Increase the pH to about 8 with a bead or two of sodium hydroxide, and heat to 60-65C. The paraformaldehyde depolymerizes ("dissolves") and you go ahead with dilution and buffering to make a 4% NBF. This information is in every histotechnical textbook published since about 1965. Your description of "crystals" in old formalin puzzles me. The paraformaldehyde usually settles out as as a finely granular white powder. John Kiernan London, Canada = = = On 26/05/15, Julio Benavides wrote: > Hi there, > > we have a bottle of formaline 40% with crystals at the bottom. Can we used it? is there any way to re-suspend the crystals? Better dispose of it? and, BTW, do you know why it crystallized? by its side there is another bottle, same batch, with no crystals. > > thanks a lot your comments > > Regards > > Julio > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From CDavis at che-east.org Wed May 27 12:25:45 2015 From: CDavis at che-east.org (Davis, Cassie) Date: Wed, 27 May 2015 13:25:45 -0400 Subject: [Histonet] formaline 40% with crystals at the bottom Message-ID: RE: we have a bottle of formaline 40% with crystals at the bottom. Can we used it? is there any way to re-suspend the crystals? Better dispose of it? and, BTW, do you know why it crystallized? by its side there is another bottle, same batch, with no crystals. > > thanks a lot your comments > > Regards > > Julio > Julio, I am a little confused, could this actually be alcoholic formalin? I've seen crystals form in alcoholic formalin when it is made with absolute rather that 50%. Normally we see white powder form when formalin dries. Cassandra Davis Histology Technician Anatomical Pathology Laboratory Saint Francis Healthcare 701 N. Clayton Street Wilmington,DE 19805 Office: 302-575-8095 Email: CDavis at che-east.org www.saintfrancishealthcare.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From jaylundgren at gmail.com Wed May 27 14:23:01 2015 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 27 May 2015 14:23:01 -0500 Subject: [Histonet] formaline 40% with crystals at the bottom In-Reply-To: References: Message-ID: Cover it loosely, and warm it up a little with a stir bar, under a hood. You should be able to tell the difference between alcoholic and aqueous formalin by smell. Why isn't it labelled? Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Wed, May 27, 2015 at 12:25 PM, Davis, Cassie wrote: > RE: > we have a bottle of formaline 40% with crystals at the bottom. Can we used > it? is there any way to re-suspend the crystals? Better dispose of it? and, > BTW, do you know why it crystallized? by its side there is another bottle, > same batch, with no crystals. > > > > thanks a lot your comments > > > > Regards > > > > Julio > > > Julio, > > I am a little confused, could this actually be alcoholic formalin? I've > seen crystals form in alcoholic formalin when it is made with absolute > rather that 50%. Normally we see white powder form when formalin dries. > > Cassandra Davis > Histology Technician > Anatomical Pathology Laboratory > Saint Francis Healthcare > 701 N. Clayton Street > Wilmington,DE 19805 > Office: 302-575-8095 > Email: CDavis at che-east.org > www.saintfrancishealthcare.org > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Timothy.Morken at ucsf.edu Wed May 27 17:54:43 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 27 May 2015 22:54:43 +0000 Subject: [Histonet] Lead Histotechnologist opening at UC San Francisco Medical Center Message-ID: <761E2B5697F795489C8710BCC72141FF36836F99@ex07.net.ucsf.edu> A position is open for a lead technologist at UCSF Medical Center, San Francisco The hours will be early morning to mid-morning. Website: http://jobs.ucsfmedicalcenter.org/ Search "Histotechnologist" Or Job ID 8737 Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken at ucsfmedctr.org Job Description Job ID: 8737 Job Title: Lead Histotechnologist - Pathology-Morgue (HISTO TCHNO LD) Job Code: 9057 Department: Pathology-Morgue Location: Mount Zion Full/Part Time: Full-Time Appointment Type: Career Shift: 8-hour Variable Weekly Hours: 40 ; 100% Union Information: This classification is represented by a union Our legacy of unsurpassed patient care and unceasing mission to integrate high-tech medical research with clinical operations has led to our prestigious standing as one of the top 10 hospitals in the nation according to U.S. News & World Report. As a premier health care institution dedicated to advancing health worldwide, UCSF Medical Center can also be the best place to advance and shape your career. The medical center's employees are one of the most important reasons why we are recognized as one of the nation's best hospitals. To work at UCSF Medical Center is to be part of an institution that provides the highest caliber of care to patients; a nurturing, dynamic and team-oriented atmosphere in which to best use your skills and talents. Job Summary Under supervision (HT-I, II level) by Senior-level technologists, Lead technologist and the Histology Supervisor, or direction (HT-III) by Lead technologist and the Histology Supervisor, or direction (HT-Lead) by the Histology Supervisor, the incumbent serves as a Histotechnologist in the Histology laboratory. Duties include tissue processing, embedding, paraffin sectioning, H&E staining, Special Staining, specimen receipt and accessioning, Laboratory information system operation, Quality Assurance record keeping, instrument maintenance, intra-operative frozen sections, and other technical duties as assigned, including coverage in the Immunohistochemistry laboratory and Grossing lab as determined by the Lab Manager. Rotates weekly between workstations within the lab. Work schedule is variable to include Saturdays and holiday coverage as scheduled. Incumbent must be able to flex work hours as needed to meet Department operational needs and cover work rotations. The flexibility to orient and work at all UCSF Medical Center locations is required. LIVING PRIDE STANDARDS Service Excellence * Demonstrates service excellence by following the Everyday PRIDE Guide with the UCSF Medical Center standards and expectations for communication and behavior. These standards and expectations convey specific behavior associated with the Medical Center's values: Professionalism, Respect, Integrity, Diversity and Excellence, and provide guidance on how we communicate with patients, visitors, faculty, staff, and students, virtually everyone, every day and with every encounter. These standards include, but are not limited to: personal appearance, acknowledging and greeting all patients and families, introductions using AIDET, managing up, service recovery, managing delays and expectations, phone standards, electronic communication, team work, cultural sensitivity and competency. * Uses effective communication skills with patients and staff; demonstrates proper telephone techniques and etiquette; acts as an escort to any patient or family member needing directions; shows sensitivity to differences of culture; demonstrates a positive and supportive manner in which patients / families/ colleagues perceive interactions as positive and supportive. Exhibits team work skills to positively acknowledge and recognize other colleagues, and uses personal experiences to model and teach Living PRIDE standards. * Exhibits tact and professionalism in difficult situations according to PRIDE Values and Practices * Demonstrates an understanding of and adheres to privacy, confidentiality, and security policies and procedures related to Protected Health Information (PHI) or other sensitive and personal information. * Demonstrates an understanding of and adheres to safety and infection control policies and procedures. * Assumes accountability for improving quality metrics associated with department/unit and meeting organizational/departmental targets. Work Environment * Keeps working areas neat, orderly and clutter-free, including the hallways. Adheres to cleaning processes and puts things back where they belong. Removes and reports broken equipment and furniture. * Picks up and disposes of any litter found throughout entire facility. * Posts flyers and posters in designated areas only; does not post on walls, doors or windows. * Knows where the Environment of Care Manual is kept in department; corrects or reports unsafe conditions to the appropriate departments. * Protects the physical environment and equipment from damage and theft. Required Qualifications * Associate degree or at least 60 semester hours of academic credit from a regionally accredited college/university, with a combination of 12 semester hours of biology and chemistry, AND five years of experience as a histotechnologist in a comparable high-volume hospital histology laboratory within the last seven years, including senior-level experience * Demonstrated high-volume, high-quality sectioning and staining skills * Ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines * Excellent interpersonal and communication skills * ASCP certification: HT licensed or eligible required (candidate will be expected to obtain certification within 1 year), HTL desirable * Demonstrated leadership and organization skills * Extensive knowledge and outstanding skill in all areas of histology up to and including: tissue processing, paraffin embedding, sectioning, manual and automated Special Stains, manual and automated immunohistochemical and in-situ hybridization staining * This tech must be technically savvy and have the ability to trouble shoot all areas of histology * The flexibility to orient and work at all UCSF Medical Center locations Preferred Qualifications * 5 - 10 years experience as a Histotechnologist in a large Hospital Laboratory * ASCP certification: HTL-licensed strongly preferred. Previous leadership experience in a hospital laboratory * Familiarity with lean, six sigma and 5S production techniques Licensure/Certification * ASCP certification: HT licensed Equal Employment Opportunity The University of California San Francisco is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, or protected veteran status. Further information about the University of California, San Francisco, is available at diversity.ucsf.edu. UCSF seeks candidates whose skills, and personal and professional experience, have prepared them to contribute to our commitment to diversity and excellence, and the communities we serve. From amylee053 at gmail.com Wed May 27 18:03:21 2015 From: amylee053 at gmail.com (Amy Lee) Date: Wed, 27 May 2015 16:03:21 -0700 Subject: [Histonet] Thanks! Message-ID: Thanks to all who responded to my question regarding PAS staining! We got much better staining after following you guys suggestion: a. we purchased reagent from American Mastertech b. we used 1% periodic acid c. we rinse slides under warm water after Schiff's step. Thanks again, Amy From timma at dxandtx.com Thu May 28 08:51:26 2015 From: timma at dxandtx.com (Timm, Amber L) Date: Thu, 28 May 2015 08:51:26 -0500 Subject: [Histonet] Opening of Uterus' and Colons Message-ID: Good morning- I am wondering if are there regulations on who is able to open uterus' and colons? We have a non-Histo tech lab assistant in our laboratory and am unable to find any regs. on if he can open these for placing in formalin. Thoughts please. Thank you Amber Timm, MLS (ASCP)CM ________________________________ CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From wbenton at cua.md Thu May 28 09:13:04 2015 From: wbenton at cua.md (Walter Benton) Date: Thu, 28 May 2015 10:13:04 -0400 Subject: [Histonet] Opening of Uterus' and Colons In-Reply-To: References: Message-ID: <0B8979A204680A42B93A52B486088CD94288273765@CUAEXH1.GCU-MD.local> Here is a link to the CLIA qualifications for testing personnel. I would imagine that staff member you are describing is not qualified to open these specimens for fixation. http://www.gpo.gov/fdsys/granule/CFR-2011-title42-vol5/CFR-2011-title42-vol5-sec493-1489 Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: Timm, Amber L [mailto:timma at dxandtx.com] Sent: Thursday, May 28, 2015 9:51 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Opening of Uterus' and Colons Good morning- I am wondering if are there regulations on who is able to open uterus' and colons? We have a non-Histo tech lab assistant in our laboratory and am unable to find any regs. on if he can open these for placing in formalin. Thoughts please. Thank you Amber Timm, MLS (ASCP)CM ________________________________ CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From CObregon at mhs.net Thu May 28 09:36:55 2015 From: CObregon at mhs.net (Obregon, Cecilia) Date: Thu, 28 May 2015 14:36:55 +0000 Subject: [Histonet] MDM2 and CDK4 Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F4F870BB6@MHSEXMB03.mhs.net> Can someone recommend a vendor and IHC protocol for MDM2 and CDK4? I have come accross these as probes from a couple of our vendor's website, but we want to try these as IHC products. Any suggestions will be greatly appreciated. Thanks for all your help. Cecilia M. Obregon Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. From bcooper at chla.usc.edu Thu May 28 12:43:22 2015 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Thu, 28 May 2015 17:43:22 +0000 Subject: [Histonet] Adenovirus controls In-Reply-To: References: Message-ID: Good morning Histonet, It's been about a month since I last sent out an inquiry about adenovirus control material, and I struck out badly with only one response! Does anyone have a spare adenovirus block they'd be willing to trade for something else they might need? We have a lot of control material available for special stains--Fungus, Gram +/-, AFB, etc . . . For IHC, we have Eber and TONS and TONS of Tonsil! You can contact me offline if you're more comfortable doing so. Any help would be greatly appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357???? bcooper at chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From alkhenaizik at gmail.com Thu May 28 12:46:57 2015 From: alkhenaizik at gmail.com (kalkhenaizi .) Date: Thu, 28 May 2015 20:46:57 +0300 Subject: [Histonet] Control Blocks for IHC Message-ID: Hello Histonetters, I am looking for control blocks for IHC. I can trade with the blocks I have or buy from providers. Any help is appreciated. Thanks, Kadhem Lab Manager ExpressMed Labs Bahrain From suetp918 at comcast.net Thu May 28 13:30:27 2015 From: suetp918 at comcast.net (Sue) Date: Thu, 28 May 2015 18:30:27 +0000 (UTC) Subject: [Histonet] Control Blocks for IHC In-Reply-To: References: Message-ID: <1402266704.3520710.1432837827669.JavaMail.zimbra@comcast.net> what kind of controls, ie stains TJUH Sue From BDeBrosse-Serra at isisph.com Thu May 28 16:03:13 2015 From: BDeBrosse-Serra at isisph.com (Bea DeBrosse-Serra) Date: Thu, 28 May 2015 21:03:13 +0000 Subject: [Histonet] Thank you everyone who responded to my service equipment request Message-ID: Thank you! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 From Sheila.Tapper at EssentiaHealth.org Fri May 29 08:29:42 2015 From: Sheila.Tapper at EssentiaHealth.org (Tapper, Sheila J.) Date: Fri, 29 May 2015 13:29:42 +0000 Subject: [Histonet] Lifelink training in the morgue Message-ID: Lifelink will be using our morgue again this evening for training of their staff. I am moving the empty vial containers into the processor room to give them enough room for their crowd. The will be done in the early evening - so if the afternoon folks could move them back when they leave - that would be great...I worry that they will be tossed by EVS if they stay in the other room... Thanks! ___________________________________________ Sheila Tapper Anatomic Pathology Supervisor Essentia Health SMDC Clinical Laboratory | SMMC Pathology 3W 407 East Third Street, Duluth, MN 55805 P: 218-786-5472 | F: 218-786-2369 sheila.tapper at essentiahealth.org From Sheila.Tapper at EssentiaHealth.org Fri May 29 08:33:23 2015 From: Sheila.Tapper at EssentiaHealth.org (Tapper, Sheila J.) Date: Fri, 29 May 2015 13:33:23 +0000 Subject: [Histonet] Recall: Lifelink training in the morgue Message-ID: Tapper, Sheila J. would like to recall the message, "Lifelink training in the morgue". From j.benavides at eae.csic.es Fri May 29 08:35:19 2015 From: j.benavides at eae.csic.es (Julio Benavides) Date: Fri, 29 May 2015 15:35:19 +0200 Subject: [Histonet] PAS-diastase staining In-Reply-To: References: Message-ID: <55686B16.1060008@eae.csic.es> Hi there, I am trying to do a PAA-diastase in formalin-fixed embedded samples (ovine liver). I would be most grateful if someone could give me a hand in: -Protocol (enzyme concentration, time of digestion...) for diastase digestion (I am assuming that PAS afterwards is the normal protocol for PAS) -A commercial source for diastase working in these samples. Is the porcine amylase from sigma any good? cheaper options? As always, thank you very much for all your comments Cheers Julio From Kristopher.Kalleberg at unilever.com Fri May 29 08:56:15 2015 From: Kristopher.Kalleberg at unilever.com (Kalleberg, Kristopher) Date: Fri, 29 May 2015 13:56:15 +0000 Subject: [Histonet] anti- antibodies Message-ID: Hello All, Has anyone else noticed that recently it seems as though almost all companies that are selling antibodies are calling them anti- antibodies (anti-tyrosinase, anti-Ki67, etc). Why have companies made this change? Any insight to this will be greatly appreciated. Thanks in advance. Kris From rjbuesa at yahoo.com Fri May 29 10:16:56 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 29 May 2015 15:16:56 +0000 (UTC) Subject: [Histonet] PAS-diastase staining In-Reply-To: <55686B16.1060008@eae.csic.es> References: <55686B16.1060008@eae.csic.es> Message-ID: <1144334360.1173004.1432912616706.JavaMail.yahoo@mail.yahoo.com> Sigma porcine diastase is very good.A cheaper option is very disgusting but had been done?for decades, just spit on the section but that will carry bacteria and, although I have seen doing it, I have never done it.Ren? J.? On Friday, May 29, 2015 10:01 AM, Julio Benavides wrote: Hi there, I am trying to do a PAA-diastase in formalin-fixed embedded samples (ovine liver). I would be most grateful if someone could give me a hand in: -Protocol (enzyme concentration, time of digestion...) for diastase digestion (I am assuming that PAS afterwards is the normal protocol for PAS) -A commercial source for diastase working in these samples. Is the porcine amylase from sigma any good? cheaper options? As always, thank you very much for all your comments Cheers Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet On Friday, May 29, 2015 10:01 AM, Julio Benavides wrote: Hi there, I am trying to do a PAA-diastase in formalin-fixed embedded samples (ovine liver). I would be most grateful if someone could give me a hand in: -Protocol (enzyme concentration, time of digestion...) for diastase digestion (I am assuming that PAS afterwards is the normal protocol for PAS) -A commercial source for diastase working in these samples. Is the porcine amylase from sigma any good? cheaper options? As always, thank you very much for all your comments Cheers Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet On Friday, May 29, 2015 10:01 AM, Julio Benavides wrote: Hi there, I am trying to do a PAA-diastase in formalin-fixed embedded samples (ovine liver). I would be most grateful if someone could give me a hand in: -Protocol (enzyme concentration, time of digestion...) for diastase digestion (I am assuming that PAS afterwards is the normal protocol for PAS) -A commercial source for diastase working in these samples. Is the porcine amylase from sigma any good? cheaper options? As always, thank you very much for all your comments Cheers Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Royl1 at LabCorp.com Fri May 29 10:37:07 2015 From: Royl1 at LabCorp.com (Roy, Lisa) Date: Fri, 29 May 2015 15:37:07 +0000 Subject: [Histonet] PAS-diastase staining In-Reply-To: <1144334360.1173004.1432912616706.JavaMail.yahoo@mail.yahoo.com> References: <55686B16.1060008@eae.csic.es> <1144334360.1173004.1432912616706.JavaMail.yahoo@mail.yahoo.com> Message-ID: I have been using the spit method for years with great results. Depar slide to water, add saliva directly to slide for 10 minutes, rinse and stain PAS as usual. Also American MasterTech has diastase malt that works well. Lisa -----Original Message----- From: Rene J Buesa [mailto:rjbuesa at yahoo.com] Sent: Friday, May 29, 2015 11:17 AM To: Julio Benavides; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS-diastase staining Sigma porcine diastase is very good.A cheaper option is very disgusting but had been done?for decades, just spit on the section but that will carry bacteria and, although I have seen doing it, I have never done it.Ren? J.? On Friday, May 29, 2015 10:01 AM, Julio Benavides wrote: Hi there, I am trying to do a PAA-diastase in formalin-fixed embedded samples (ovine liver). I would be most grateful if someone could give me a hand in: -Protocol (enzyme concentration, time of digestion...) for diastase digestion (I am assuming that PAS afterwards is the normal protocol for PAS) -A commercial source for diastase working in these samples. Is the porcine amylase from sigma any good? cheaper options? As always, thank you very much for all your comments Cheers Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet On Friday, May 29, 2015 10:01 AM, Julio Benavides wrote: Hi there, I am trying to do a PAA-diastase in formalin-fixed embedded samples (ovine liver). I would be most grateful if someone could give me a hand in: -Protocol (enzyme concentration, time of digestion...) for diastase digestion (I am assuming that PAS afterwards is the normal protocol for PAS) -A commercial source for diastase working in these samples. Is the porcine amylase from sigma any good? cheaper options? As always, thank you very much for all your comments Cheers Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet On Friday, May 29, 2015 10:01 AM, Julio Benavides wrote: Hi there, I am trying to do a PAA-diastase in formalin-fixed embedded samples (ovine liver). I would be most grateful if someone could give me a hand in: -Protocol (enzyme concentration, time of digestion...) for diastase digestion (I am assuming that PAS afterwards is the normal protocol for PAS) -A commercial source for diastase working in these samples. Is the porcine amylase from sigma any good? cheaper options? As always, thank you very much for all your comments Cheers Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. From j.benavides at eae.csic.es Fri May 29 10:30:57 2015 From: j.benavides at eae.csic.es (Julio Benavides) Date: Fri, 29 May 2015 17:30:57 +0200 Subject: [Histonet] PAS-diastase staining and formalin "crystals" In-Reply-To: <1144334360.1173004.1432912616706.JavaMail.yahoo@mail.yahoo.com> References: <55686B16.1060008@eae.csic.es> <1144334360.1173004.1432912616706.JavaMail.yahoo@mail.yahoo.com> Message-ID: <55688631.8080106@eae.csic.es> Hi there, thank you all very much for your comments and protocols on the PAS-diastase. Although funny thing that cheaper option, I think I stick to the Sigma enzyme first. Update on the formalin "crystals". It is actually, as you suggested, white granular precipitate. There was so much of it that it formed some big "crusts" (which led me to think about crystals when looking trough the bottle...). The formalin was aqueous solution (stabilised with 10% methanol). I have tried to depolymerize it heating it with a couple of NaOH2 beads but to no success. Thank you very much for your comments. Regards Julio From Rhonda.Gregoire at gov.mb.ca Fri May 29 12:09:28 2015 From: Rhonda.Gregoire at gov.mb.ca (Gregoire, Rhonda (MAFRD)) Date: Fri, 29 May 2015 17:09:28 +0000 Subject: [Histonet] Warthin Starry "snowflakes" Message-ID: We are have been trying to troubleshoot our Warthin Starry stain. We have been seeing "snowflakes" appearing on the slides. The pathologists sometimes have a hard time as they sort of look like they could be spirochetes. Please help!! Thanks! Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 From lblazek at digestivespecialists.com Fri May 29 13:23:00 2015 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Fri, 29 May 2015 14:23:00 -0400 Subject: [Histonet] plants in the lab Message-ID: <5A2BD13465E061429D6455C8D6B40E3917421260A6@IBMB7Exchange.digestivespecialists.com> Happy Friday all! Does anyone have documentation of the benefit of having plants in the lab? I know this was discusses quite a while ago but I can't find references for it. Any help would be appreciated. Thanks, Linda From sally.norton at seattlechildrens.org Fri May 29 13:39:05 2015 From: sally.norton at seattlechildrens.org (Norton, Sally) Date: Fri, 29 May 2015 18:39:05 +0000 Subject: [Histonet] plants in the lab In-Reply-To: <5A2BD13465E061429D6455C8D6B40E3917421260A6@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E3917421260A6@IBMB7Exchange.digestivespecialists.com> Message-ID: <1357F84B33D39A46BA015A8EC6ABCBD040ED44A4@PPWEXD01a.childrens.sea.kids> We have them, but have not documented the benefit, other than our enjoyment of them, especially when the violets and orchids bloom! Sally Norton Seattle Children's Hospital -----Original Message----- From: Blazek, Linda [mailto:lblazek at digestivespecialists.com] Sent: Friday, May 29, 2015 11:23 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] plants in the lab Happy Friday all! Does anyone have documentation of the benefit of having plants in the lab? I know this was discusses quite a while ago but I can't find references for it. Any help would be appreciated. Thanks, Linda _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From ChaseM at childrensdayton.org Fri May 29 13:54:05 2015 From: ChaseM at childrensdayton.org (Matthew Chase) Date: Fri, 29 May 2015 18:54:05 +0000 Subject: [Histonet] Plants in the lab Message-ID: <187FB57B597CB24F9F59F357264999F70128C949C4@PEXCHNGDAG01.cmc-dayton.org> How about documentation saying it's ok to have plants in the lab. We have been told that Dan the Safety Man has documentation stating "they are not allowed in the lab". Matt ________________________________ NOTICE: The information contained in this e-mail and any accompanying documents is intended for the sole use of the recipient to whom it is addressed, and may contain information that is privileged, confidential, and prohibited from disclosure under applicable law. If you are not the intended recipient, or authorized to receive this on behalf of the recipient, you are hereby notified that any review, use, disclosure, copying, or distribution is prohibited. If you are not the intended recipient(s), please contact the sender by e-mail and promptly destroy the original message. Thank you. From jvickroy at SpringfieldClinic.com Fri May 29 14:13:04 2015 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Fri, 29 May 2015 19:13:04 +0000 Subject: [Histonet] Billing Message-ID: <9B1A1501A800064397369BD8072E6BCA064061C9@E2K10DB.springfieldclinic.com> Just need a reminder: If a pathologist orders the same several IHC stains on two blocks from the same specimen am I correct to think that we can only charge the stains on one of the blocks? 88342 and the remainder 88341's Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From mthomas at littonlab.com Fri May 29 14:21:50 2015 From: mthomas at littonlab.com (Marla Thomas) Date: Fri, 29 May 2015 19:21:50 +0000 Subject: [Histonet] Plants in the lab In-Reply-To: <187FB57B597CB24F9F59F357264999F70128C949C4@PEXCHNGDAG01.cmc-dayton.org> References: <187FB57B597CB24F9F59F357264999F70128C949C4@PEXCHNGDAG01.cmc-dayton.org> Message-ID: www.livescience.com/38445-indoor-plants-clean-air.html According to the above link, plants can absorb chemical such as formalin. We were always told they helped clean the air. Maybe it is really true! Marla -----Original Message----- From: Matthew Chase [mailto:ChaseM at childrensdayton.org] Sent: Friday, May 29, 2015 1:54 PM To: 'Histonet at lists.utsouthwestern.edu' Subject: [Histonet] Plants in the lab How about documentation saying it's ok to have plants in the lab. We have been told that Dan the Safety Man has documentation stating "they are not allowed in the lab". Matt ________________________________ NOTICE: The information contained in this e-mail and any accompanying documents is intended for the sole use of the recipient to whom it is addressed, and may contain information that is privileged, confidential, and prohibited from disclosure under applicable law. If you are not the intended recipient, or authorized to receive this on behalf of the recipient, you are hereby notified that any review, use, disclosure, copying, or distribution is prohibited. If you are not the intended recipient(s), please contact the sender by e-mail and promptly destroy the original message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward at wakehealth.edu Fri May 29 14:29:01 2015 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Fri, 29 May 2015 19:29:01 +0000 Subject: [Histonet] Billing In-Reply-To: <9B1A1501A800064397369BD8072E6BCA064061C9@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA064061C9@E2K10DB.springfieldclinic.com> Message-ID: Yes that is how we do it as well. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward at wakehealth.edu ? ? ? -----Original Message----- From: Vickroy, James [mailto:jvickroy at SpringfieldClinic.com] Sent: Friday, May 29, 2015 3:13 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Billing Just need a reminder: If a pathologist orders the same several IHC stains on two blocks from the same specimen am I correct to think that we can only charge the stains on one of the blocks? 88342 and the remainder 88341's Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems at emoryhealthcare.org Fri May 29 19:02:50 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Sat, 30 May 2015 00:02:50 +0000 Subject: [Histonet] Billing In-Reply-To: <9B1A1501A800064397369BD8072E6BCA064061C9@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA064061C9@E2K10DB.springfieldclinic.com> Message-ID: <60D749AF-D5F4-4F59-8739-D0F95F4F9542@emoryhealthcare.org> Correct > On May 29, 2015, at 3:13 PM, Vickroy, James wrote: > > > Just need a reminder: > > If a pathologist orders the same several IHC stains on two blocks from the same specimen am I correct to think that we can only charge the stains on one of the blocks? 88342 and the remainder 88341's > > Jim > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvickroy at SpringfieldClinic.com > > > > This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From kaarrington at anthc.org Fri May 29 20:48:02 2015 From: kaarrington at anthc.org (Arrington, Karla A) Date: Sat, 30 May 2015 01:48:02 +0000 Subject: [Histonet] Decontaminate of Benchmark Message-ID: What dilution does one use for decontaminating the XT? Manual states 3L but I think this is way too much. How much Lysol to water? Thanks!! Sent from my iPhone From MICHELLE.LAMPHERE at childrens.com Sun May 31 07:36:17 2015 From: MICHELLE.LAMPHERE at childrens.com (Michelle Lamphere) Date: Sun, 31 May 2015 12:36:17 +0000 Subject: [Histonet] plants in the lab In-Reply-To: References: Message-ID: Our hospital Safety and Infection Control departments have policies in place prohibiting any potted plants from being in the hospital, anywhere. We can have them if they are only in water, but the soil presents an infection control issue for patients because of potential mildew, mold, spores, etc. Michelle Lamphere Senior Tech, Histology Anatomic Pathology O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere at childrens.com 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 Message: 2 Date: Fri, 29 May 2015 14:23:00 -0400 From: "Blazek, Linda" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] plants in the lab Message-ID: <5A2BD13465E061429D6455C8D6B40E3917421260A6 at IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Happy Friday all! Does anyone have documentation of the benefit of having plants in the lab? I know this was discusses quite a while ago but I can't find references for it. Any help would be appreciated. Thanks, Linda Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at privacy at childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From linda.prasad at health.nsw.gov.au Sun May 31 20:20:00 2015 From: linda.prasad at health.nsw.gov.au (Linda Prasad (SCHN)) Date: Mon, 1 Jun 2015 01:20:00 +0000 Subject: [Histonet] PAS-diastase staining In-Reply-To: References: <55686B16.1060008@eae.csic.es> <1144334360.1173004.1432912616706.JavaMail.yahoo@mail.yahoo.com> Message-ID: <1217DDB3D7DE5E418E3D560A268EABD0E0E91971@xmdb03.nch.kids> We have been making our own diastase solution for years now. Works extremely well. The diastase is very easy to make in house. Here is the protocol for making your own diastase Principle: The enzyme solution is applied to one of two sections of the tissue (preferably consecutive sections) and then both are stained by the PAS method. The presence and relative amount of glycogen in the sections can be determined by examining the extent of loss of staining in the enzyme treated section as compared with the untreated section. This amylase reagent has a long shelf life and uses an incubation time of 10 minutes at room temperature. It is suitable for formalin and Brazil?s fixed paraffin sections as well as air-dried and ethanol fixed frozen sections. Controls: Liver containing glycogen Reagents: 1. Amylase Reagent Warning: Harmful, contains azide ? see MSDS Alpha Amylase from Bacillus Subtilis (Sigma Cat No 10070,) 1g Oxoid PBS Tablets (Cat No BR14a) 1 tablet Distilled water 100ml Sodium Azide 0.1g This solution, once prepared is stored at 4oC when not in use. A recycled antibody dropper bottle (often used in commercial immunoperoxidase kits) is useful for storage and application. 2. PAS Reagents (see PAS Stain) Warning: Suspected Carcinogen ? see MSDS Procedure: 1. Dewax and hydrate paraffin sections, hydrate frozen sections. 2. For amylase digestion, place slides on a rack, cover sections with amylase solution and allow to incubate for 10 minutes at room temperature. 3. Wash slides well in water. 4. Place slides in 1% periodic acid 10 minutes. 5. Wash slides well in water. 6. Rinse slides in distilled water. 7. Place in Schiff?s reagent 10 minutes. 8. Rinse slides in distilled water and then wash slides in tap water 3 minutes. 9. Counterstain slides with haematoxylin, differentiate and blue. 10. Dehydrate, clear and mount. Results: ? Glycogen is extracted and so loss of PAS positive staining will occur in the enzyme treated section. ? Mucopolysaccharides are not extracted and so staining will be the same in both sections. Reference: V-M. Mangan, V. Farago, M. Kelly, and A. F. Henwood (2002) " An Amylase Reagent with a Long Shelf Life for the Removal of Glycogen from Tissue Sections" J Histotechnol. 25(3): 153-4. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad at health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???Please consider the environment before printing this email. -----Original Message----- From: Roy, Lisa [mailto:Royl1 at LabCorp.com] Sent: Saturday, 30 May 2015 1:37 AM To: Rene J Buesa; Julio Benavides; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS-diastase staining I have been using the spit method for years with great results. Depar slide to water, add saliva directly to slide for 10 minutes, rinse and stain PAS as usual. Also American MasterTech has diastase malt that works well. Lisa -----Original Message----- From: Rene J Buesa [mailto:rjbuesa at yahoo.com] Sent: Friday, May 29, 2015 11:17 AM To: Julio Benavides; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] PAS-diastase staining Sigma porcine diastase is very good.A cheaper option is very disgusting but had been done?for decades, just spit on the section but that will carry bacteria and, although I have seen doing it, I have never done it.Ren? J.? On Friday, May 29, 2015 10:01 AM, Julio Benavides wrote: Hi there, I am trying to do a PAA-diastase in formalin-fixed embedded samples (ovine liver). I would be most grateful if someone could give me a hand in: -Protocol (enzyme concentration, time of digestion...) for diastase digestion (I am assuming that PAS afterwards is the normal protocol for PAS) -A commercial source for diastase working in these samples. Is the porcine amylase from sigma any good? cheaper options? As always, thank you very much for all your comments Cheers Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet On Friday, May 29, 2015 10:01 AM, Julio Benavides wrote: Hi there, I am trying to do a PAA-diastase in formalin-fixed embedded samples (ovine liver). I would be most grateful if someone could give me a hand in: -Protocol (enzyme concentration, time of digestion...) for diastase digestion (I am assuming that PAS afterwards is the normal protocol for PAS) -A commercial source for diastase working in these samples. Is the porcine amylase from sigma any good? cheaper options? As always, thank you very much for all your comments Cheers Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet On Friday, May 29, 2015 10:01 AM, Julio Benavides wrote: Hi there, I am trying to do a PAA-diastase in formalin-fixed embedded samples (ovine liver). I would be most grateful if someone could give me a hand in: -Protocol (enzyme concentration, time of digestion...) for diastase digestion (I am assuming that PAS afterwards is the normal protocol for PAS) -A commercial source for diastase working in these samples. Is the porcine amylase from sigma any good? cheaper options? As always, thank you very much for all your comments Cheers Julio _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. 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