From jkiernan <@t> uwo.ca Sun Mar 1 15:27:49 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sun Mar 1 15:27:53 2015 Subject: [Histonet] Re: CBG recyler and recycled Formalin In-Reply-To: <7390bae744b01.54f3841c@uwo.ca> References: <7380a2641cad0.54f29b65@uwo.ca> <7220d7051fb3b.54f29ba2@uwo.ca> <722092b61a271.54f29be0@uwo.ca> <731089be197e2.54f29c1e@uwo.ca> <731085c91b650.54f29c5b@uwo.ca> <7310f18019918.54f29c99@uwo.ca> <7300be8a1ae61.54f29cd8@uwo.ca> <7300abb019114.54f29d16@uwo.ca> <7220ca1b19767.54f29e44@uwo.ca> <7300ea051cef4.54f29e82@uwo.ca> <730096fd1e053.54f29ec0@uwo.ca> <73009a6b1fac9.54f29efe@uwo.ca> <7370f78e1d2b8.54f29f3c@uwo.ca> <72d0aea21d6af.54f29f7a@uwo.ca> <7390920e186fd.54f29fb8@uwo.ca> <7310ec5a1dfbc.54f29ff6@uwo.ca> <7300f7301b224.54f2a034@uwo.ca> <7310a62b19228.54f2a072@uwo.ca> <7310cb851c585.54f2a0ec@uwo.ca> <71f0df811d6d0.54f2a12a@uwo.ca> <7300d6e51c1cf.54f2a168@uwo.ca> <73e0d4d819e79.54f2a1a6@uwo.ca> <73e0fe121cdbe.54f2a1e4@uwo.ca> <7310c78c1b90b.54f2a222@uwo.ca> <73e0d7461d543.54f2a38c@uwo.ca> <73e0e3b319baa.54f2a3ca@uwo.ca> <73e0df021c7a8.54f2a408@uwo.ca> <7370c04e185ac.54f2a4be@uwo.ca> <737080751a411.54f2a4fd@uwo.ca> <73709ab61cbfc.54f2a53b@uwo.ca> <73c0ab211d8e9.54f2a579@uwo.ca> <73c0fb191f43a.54f2a5b7@uwo.ca> <72d084c11aebf.54f2a5f5@uwo.ca> <71f095ff18036.54f2a633@uwo.ca> <71f0d2dd1d0f2.54f2a6ad@uwo.ca> <7300dbf319158.54f2a727@uwo.ca> <7370bc961d3b1.54f2a7a1@uwo.ca> <7380f06c19258.54f2a7df@uwo.ca> <7380da041d6ba.54f2a810@uwo.ca> <73e0b0a7446e3.54f3822b@uwo.ca> <7390872741deb.54f3826a@uwo.ca> <71b0ff64455b8.54f382a8@uwo.ca> <72e0c0a145d41.54f382e6@uwo.ca> <73e0998444916.54f38324@uwo.ca> <73e0e75c45946.54f38362@uwo.ca> <7160b7d8428fa.54f383a0@uwo.ca> <7160af48447ea.54f383de@uwo.ca> <7390bae744b01.54f3841c@uwo.ca> Message-ID: <7250ef334755a.54f33e05@uwo.ca> Bob Richmond makes an important point that should also be made clear by any company selling apparatus to recover formaldehyde by distillation of diluted formalin. The recovered product recovered from an aqueous fixative will be an aqueous solution of formaldehyde (and its low polymers), of uncertain concentration. Distillation of full-strength formalin (37%w/w = 40%w/v formaldehyde), done at atmospheric pressure, yields 20-30% of formaldehyde in the distillate, and leaves a higher concentration of formaldehyde and polymerization products in the still. Vacuum distillation or pressure distillation can change the yields to favour the collection of hydrated formaldehyde (methylene glycol) in the distillate. I'm summarizing Chapter 8 in Walker, JF (1964) Formaldehyde, 3rd edition. ISBN 0882752189. Walker's Chapter 8 tabulates conditions for distilling formaldehyde from various concentrations, and takes into account the methanol (about 5%, included in formalin to retard polymerization). The notion of an expensive machine for recycling formalin in a histology lab makes little sense. Why not simply filter (if necessary) and re-use the fixative after removing the specimen? It is still neutral, buffered formaldehyde. John Kiernan London, Canada = = = On 28/02/15, Bob Richmond wrote: > Ryan Roy HTL (ASCP) in Manchester NH asks: > > >>We are getting a new CBG that recycles xylene , alcohol, and formalin. We > purchase buffered formalin. Does anyone know if after recycling the > recycled formalin would or would not need be re-buffered?<< > > If you distill buffered formalin, the formaldehyde is going to distill > over, but not the buffer phosphate, which will remain in the still pot. I > suppose you can buy phosphate mixtures to make Lillie's neutral buffered > formalin anew, using your recycled formaldehyde. > > I think you also have to measure the concentration of formalehyde in the > distillate, and dilute accordingly. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Valerie.Hannen <@t> parrishmed.com Mon Mar 2 05:00:03 2015 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Mar 2 05:01:40 2015 Subject: [Histonet] Re: CBG recyler and recycled Formalin In-Reply-To: <7250ef334755a.54f33e05@uwo.ca> References: <7380a2641cad0.54f29b65@uwo.ca> <7220d7051fb3b.54f29ba2@uwo.ca> <722092b61a271.54f29be0@uwo.ca> <731089be197e2.54f29c1e@uwo.ca> <731085c91b650.54f29c5b@uwo.ca> <7310f18019918.54f29c99@uwo.ca> <7300be8a1ae61.54f29cd8@uwo.ca> <7300abb019114.54f29d16@uwo.ca> <7220ca1b19767.54f29e44@uwo.ca> <7300ea051cef4.54f29e82@uwo.ca> <730096fd1e053.54f29ec0@uwo.ca> <73009a6b1fac9.54f29efe@uwo.ca> <7370f78e1d2b8.54f29f3c@uwo.ca> <72d0aea21d6af.54f29f7a@uwo.ca> <7390920e186fd.54f29fb8@uwo.ca> <7310ec5a1dfbc.54f29ff6@uwo.ca> <7300f7301b224.54f2a034@uwo.ca> <7310a62b19228.54f2a072@uwo.ca> <7310cb851c585.54f2a0ec@uwo.ca> <71f0df811d6d0.54f2a12a@uwo.ca> <7300d6e51c1cf.54f2a168@uwo.ca> <73e0d4d819e79.54f2a1a6@uwo.ca> <73e0fe121cdbe.54f2a1e4@uwo.ca> <7310c78c1b90b.54f2a222@uwo.ca> <73e0d7461d543.54f2a38c@uwo.ca> <73e0e3b319baa.54f2a3ca@uwo.ca> <73e0df021c7a8.54f2a408@uwo.ca> <7370c04e185ac.54f2a4be@uwo.ca> <737080751a411.54f2a4fd@uwo.ca> <73709ab61cbfc.54f2a53b@uwo.ca> <73c0ab211d8e9.54f2a579@uwo.ca> <73c0fb191f43a.54f2a5b7@uwo.ca> <72d084c11aebf.54f2a5f5@uwo.ca> <71f095ff18036.54f2a633@uwo.ca> <71f0d2dd1d0f2.54f2a6ad@uwo.ca> <7300dbf319158.54f2a727@uwo.ca> <7370bc961d3b1.54f2a7a1@uwo.ca> <7380f06c19258.54f2a7df@uwo.ca> <7380da041d6ba.54f2a810@uwo.ca> <73e0b0a7446e3.54f3822b@uwo.ca> <7390872741deb.54f3826a@uwo.ca> <71b0ff64455b8.54f382a8@uwo.ca> <72e0c0a145d41.54f382e6@uwo.ca> <73e0998444916.54f38324@uwo.ca> <73e0e75c45946.54f38362@uwo.ca> <7160b7d8428fa.54f383a0@uwo.ca> <7160af48447ea.54f383de@uwo.ca> <7390bae744b01.54f3841c@uwo.ca> <7250ef334755a.54f33e05@uwo.ca> Message-ID: <450B7A81EDA0C54E97C53D60F00776C3233738421F@isexstore03> We have the CBG recycler and use buffered formalin. Yes, you have to re-buffer it. They sell the buffer and also give you instructions on how to re-buffer the formalin. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Sunday, March 01, 2015 4:28 PM To: Bob Richmond; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: CBG recyler and recycled Formalin Bob Richmond makes an important point that should also be made clear by any company selling apparatus to recover formaldehyde by distillation of diluted formalin. The recovered product recovered from an aqueous fixative will be an aqueous solution of formaldehyde (and its low polymers), of uncertain concentration. Distillation of full-strength formalin (37%w/w = 40%w/v formaldehyde), done at atmospheric pressure, yields 20-30% of formaldehyde in the distillate, and leaves a higher concentration of formaldehyde and polymerization products in the still. Vacuum distillation or pressure distillation can change the yields to favour the collection of hydrated formaldehyde (methylene glycol) in the distillate. I'm summarizing Chapter 8 in Walker, JF (1964) Formaldehyde, 3rd edition. ISBN 0882752189. Walker's Chapter 8 tabulates conditions for distilling formaldehyde from various concentrations, and takes into account the methanol (about 5%, included in formalin to retard polymerization). The notion of an expensive machine for recycling formalin in a histology lab makes little sense. Why not simply filter (if necessary) and re-use the fixative after removing the specimen? It is still neutral, buffered formaldehyde. John Kiernan London, Canada = = = On 28/02/15, Bob Richmond wrote: > Ryan Roy HTL (ASCP) in Manchester NH asks: > > >>We are getting a new CBG that recycles xylene , alcohol, and > >>formalin. We > purchase buffered formalin. Does anyone know if after recycling the > recycled formalin would or would not need be re-buffered?<< > > If you distill buffered formalin, the formaldehyde is going to distill > over, but not the buffer phosphate, which will remain in the still > pot. I suppose you can buy phosphate mixtures to make Lillie's neutral > buffered formalin anew, using your recycled formaldehyde. > > I think you also have to measure the concentration of formalehyde in > the distillate, and dilute accordingly. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From PREISZNE <@t> mail.etsu.edu Mon Mar 2 09:25:04 2015 From: PREISZNE <@t> mail.etsu.edu (Preiszner, Johanna) Date: Mon Mar 2 09:25:14 2015 Subject: [Histonet] need light for flotation bath Message-ID: Hi, we have a Fisher Tissue Prep 135 flotation bath and the light died. The instrument is no longer manufactured and I cant find a source for the fluorescent light tube. There are multiple baths for sale on ebay but nobody sells the light alone. Could someone help me out? Would something from Home Depo do? Thanks, Hanna From lblazek <@t> digestivespecialists.com Mon Mar 2 09:27:56 2015 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Mar 2 09:28:03 2015 Subject: [Histonet] RE: need light for flotation bath In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E3917338B94BA@IBMB7Exchange.digestivespecialists.com> I found them at Lowe's. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Preiszner, Johanna Sent: Monday, March 02, 2015 10:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] need light for flotation bath Hi, we have a Fisher Tissue Prep 135 flotation bath and the light died. The instrument is no longer manufactured and I cant find a source for the fluorescent light tube. There are multiple baths for sale on ebay but nobody sells the light alone. Could someone help me out? Would something from Home Depo do? Thanks, Hanna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ecameron1 <@t> midcoasthealth.com Mon Mar 2 09:45:15 2015 From: ecameron1 <@t> midcoasthealth.com (Cameron, Elizabeth) Date: Mon Mar 2 09:45:21 2015 Subject: [Histonet] North East Laboratory Conference Message-ID: There is a North East Laboratory Conference that is held in Portland, Maine every year, and each year I notice a lack of speakers pertaining to the fascinating subject of histology. I have taken it upon myself to recruit a few histology speakers, and I am hoping to get some talks set up for October 20, 2015. If you are interested or if you would like to recommend a speaker, please send me a message directly, and I will pass along the details of the conference. Thank you! Elizabeth M. Cameron, HT (ASCP), QIHC Lead Histologist Mid Coast Hospital 123 Medical Center Drive Brunswick, ME 04011 (207) 373-6573 From TGoins <@t> mt.gov Mon Mar 2 10:20:27 2015 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Mon Mar 2 10:21:02 2015 Subject: [Histonet] RE: need light for flotation bath In-Reply-To: References: Message-ID: Try Light Bulb Surplus at www.lightbulbsurplus.com. I got replacement bulbs for less than $5.00 rather than $75.00 from a "scientific" supply vendor. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Preiszner, Johanna Sent: Monday, March 02, 2015 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] need light for flotation bath Hi, we have a Fisher Tissue Prep 135 flotation bath and the light died. The instrument is no longer manufactured and I cant find a source for the fluorescent light tube. There are multiple baths for sale on ebay but nobody sells the light alone. Could someone help me out? Would something from Home Depo do? Thanks, Hanna _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ryan.Roy <@t> va.gov Mon Mar 2 10:32:23 2015 From: Ryan.Roy <@t> va.gov (Roy, Ryan) Date: Mon Mar 2 10:32:48 2015 Subject: [Histonet] sections blowing apart in stainer Message-ID: <15F883394EAB744E99E1C7E1B98730490178BF4ECA0B@R04BYNMSGB1.r04.med.va.gov> Hello, Cut some skins the today (3 sections one slide) and the top two sections seemed to blow apart and drift " in the stainer" (not on the bath). The bottom section looks good. This is strange as I have seen the bottom section move as a result of water or not adhering to slide. However is in this case the top two sections appear blown apart and drifted and this occurred while staining? Any thoughts appreciated. Thanks, Ryan Roy HTL (ASCP) Histology Lab Manchester VA 718 Symth Rd Manchester NH 03104 (603) 624-4366 ex 6640 Disclosure: The content of this email does not reflect the policies, views or opions of the VA. From hymclab.hymclab <@t> ministryhealth.org Mon Mar 2 11:06:37 2015 From: hymclab.hymclab <@t> ministryhealth.org (hymclab) Date: Mon Mar 2 11:07:08 2015 Subject: [Histonet] Tri-State Histology Symposium In-Reply-To: <43963a03b96143e0ad30730d9d52461c@PHUSCB-SP37MB04.genoptix.org> References: <43963a03b96143e0ad30730d9d52461c@PHUSCB-SP37MB04.genoptix.org> Message-ID: Dear Histonetters: You are invited to join the histology societies of Wisconsin, Iowa and Minnesota as we celebrate "Hats Off to Histology" at the 2015 Tri-State Histology Symposium, May 6-8 at The Madison Concourse Hotel and Governors Club in Madison, Wisconsin. For program, registration and vendor/exhibit information contact the following representatives: Wisconsin: Kathryn Stoll kstoll@mcw.edu Iowa: Judi Stasko judith.stasko@ars.usda.gov Minnesota: Lois Rowe rowe.lois@mayo.edu Vendor/Exhibit: Dawn Schneider dawn.schneider@ministryhealth.org ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From TanyaAbbott <@t> catholichealth.net Mon Mar 2 12:12:22 2015 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Mon Mar 2 12:12:46 2015 Subject: [Histonet] Body storage refrigerator temperatures Message-ID: <852F7D2C14FB464D80E182B15DB138AF4CDBF24A@CHIEX005.CHI.catholichealth.net> Does anyone else have a problem getting their body storage refrigerators to maintain the 1.1?C to 4.4?C temps to meet CAP guidelines? Thanks in advance for your responses! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From Nancy_Schmitt <@t> pa-ucl.com Mon Mar 2 12:17:33 2015 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Mon Mar 2 12:17:45 2015 Subject: [Histonet] CBG recyler and recycled Formalin Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C3601159AF15E@PEITHA.wad.pa-ucl.com> Roy- We use CBG recyclers - one for alcohol/xylene and one for formalin. The formalin is tested and with a couple simple equations you determine whether you need to add water or formalin concentrate to get back to 10%, figure out total amount of formalin and another equation to determine the amount of buffer to add. Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA ____________________________________________________________________ Date: Fri, 27 Feb 2015 14:41:51 -0500 From: "Roy, Ryan" Subject: [Histonet] CBG recyler and recycled Formalin To: "histonet@lists.utsouthwestern.edu" Message-ID: <15F883394EAB744E99E1C7E1B98730490178BF4ECA01@R04BYNMSGB1.r04.med.va.gov> Content-Type: text/plain; charset="us-ascii" Hello, We are getting a new CBG that recycles xylene , alcohol, and formalin. We purchase buffered formalin? Does anyone know if after recycling the recycled formalin would or would not need be re-buffered?? Thanks in advance, Ryan Roy HTL (ASCP) Histology Lab Manchester VA 718 Symth Rd Manchester NH 03104 (603) 624-4366 ex 6640 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From tpodawiltz <@t> lrgh.org Mon Mar 2 12:26:07 2015 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Mon Mar 2 12:26:21 2015 Subject: [Histonet] RE: Body storage refrigerator temperatures In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF4CDBF24A@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF4CDBF24A@CHIEX005.CHI.catholichealth.net> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638632616513C42@LRGHEXVS1.practice.lrgh.org> Not at the moment in New England. Sorry, I just could not resist with the way this winter has been. We did at one time and we ended up replacing the cooling unit in our morgue. Tom -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Monday, March 02, 2015 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Body storage refrigerator temperatures Does anyone else have a problem getting their body storage refrigerators to maintain the 1.1?C to 4.4?C temps to meet CAP guidelines? Thanks in advance for your responses! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From amylee779 <@t> yahoo.com Mon Mar 2 13:32:00 2015 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Mon Mar 2 13:32:04 2015 Subject: [Histonet] frozen tissue training Message-ID: <360070444.1254782.1425324720480.JavaMail.yahoo@mail.yahoo.com> Hello histonet, I am looking for a frozen tissue training session for my new employee. It includes frozen tissue harvest, storing, sectioning, etc. Could any one recommend a training session inside US? Thanks, Amy From robert.jacox <@t> thermofisher.com Mon Mar 2 13:54:35 2015 From: robert.jacox <@t> thermofisher.com (Jacox, Robert A.) Date: Mon Mar 2 13:54:49 2015 Subject: [Histonet] frozen tissue training In-Reply-To: <360070444.1254782.1425324720480.JavaMail.yahoo@mail.yahoo.com> References: <360070444.1254782.1425324720480.JavaMail.yahoo@mail.yahoo.com> Message-ID: <7B380BF8E6354F4994F20E095D43F1710B095B5BA9@USPHO-MXVS01.amer.thermo.com> Amy, I am not sure what type of tissue you are cutting but the MOHs society has a nice training course. Fundamentals of Mohs Surgery for Physicians and Mohs Technicians Thursday, November 5 ? Sunday, November 8, 2015 Robert Jacox Manager, Global Tactical Marketing Anatomic Pathology Thermo Fisher Scientific Tel: 269-544-5651 l Mobile: 269-598-0747 robert.jacox@thermofisher.com l www.thermoscientific.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Monday, March 02, 2015 2:32 PM To: histonet Subject: [Histonet] frozen tissue training Hello histonet, I am looking for a frozen tissue training session for my new employee. It includes frozen tissue harvest, storing, sectioning, etc. Could any one recommend a training session inside US? Thanks, Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pdefazio802 <@t> gmail.com Mon Mar 2 18:11:19 2015 From: pdefazio802 <@t> gmail.com (Pam DeFazio) Date: Mon Mar 2 18:11:24 2015 Subject: [Histonet] Double edge blades Message-ID: Forgive me if I have asked this before. We cannot seen to find any double edge blades that are consistently sharp. Our pathologists like to use these, and I am having a hard time finding good ones. I have tried several vendors, but they all seem to be manufactured by Personna. Am I correct? One vendor says they come from Pathco. Another from Personna, but the actual wrapper on the blade for both say Personna. Are these 2 different manufactures? Any suggestions? Pam DeFazio, HT ASCP ARMC Athens, GA From talulahgosh <@t> gmail.com Tue Mar 3 06:38:30 2015 From: talulahgosh <@t> gmail.com (Emily Brown) Date: Tue Mar 3 06:38:34 2015 Subject: [Histonet] Masson trichrome and H and E Message-ID: Hello! I am doing Masson trichrome manually (not with a machine) and I just found out the kit is $800!! I was thinking of buying the ingredients separately, but why the hell is it so expensive??? Also, the people I was going to borrow their reagents from said their aniline blue is not very good and I wanted to replace it. I only need about 250ml, what brand do you guys prefer? l know I can google this, but I want to know what you guys like and what works best. This is for mouse kidney paraffin sections, 4 to 5 microns. Another question, I did H and E and there is no eosin staining. I think the reagents are pretty old, so I thought that might be a problem. Also because my lab is cheap, they were reusing the xylenes and EtOH for both rehydrating and rehydrating. I told my boss this is probably not a good idea as the end steps will have stain in them. And I also think this is why it didn't work! The EtOH is also really old so who know is the 100% is actually even close to 100% any more. I'm buying new reagents, but if you guys think anything else would help, let me know. Also, shoutout to Ann, I know you're reading these!! Join the list!! Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" From Toni.Rathborne <@t> rwjuh.edu Tue Mar 3 07:34:31 2015 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Tue Mar 3 07:36:57 2015 Subject: [Histonet] Masson trichrome and H and E In-Reply-To: References: Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F58CA5@vap1014.win.rwjuh.edu> We have been very satisfied with special stain solutions from Poly Scientific (http://www.polyrnd.com/products/stains/general/aniline-blue---masson's-trichrome.aspx). We get an 8oz bottle for under $30. Very good customer service too. Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Tuesday, March 03, 2015 7:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Masson trichrome and H and E Hello! I am doing Masson trichrome manually (not with a machine) and I just found out the kit is $800!! I was thinking of buying the ingredients separately, but why the hell is it so expensive??? Also, the people I was going to borrow their reagents from said their aniline blue is not very good and I wanted to replace it. I only need about 250ml, what brand do you guys prefer? l know I can google this, but I want to know what you guys like and what works best. This is for mouse kidney paraffin sections, 4 to 5 microns. Another question, I did H and E and there is no eosin staining. I think the reagents are pretty old, so I thought that might be a problem. Also because my lab is cheap, they were reusing the xylenes and EtOH for both rehydrating and rehydrating. I told my boss this is probably not a good idea as the end steps will have stain in them. And I also think this is why it didn't work! The EtOH is also really old so who know is the 100% is actually even close to 100% any more. I'm buying new reagents, but if you guys think anything else would help, let me know. Also, shoutout to Ann, I know you're reading these!! Join the list!! Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mgflem <@t> gmail.com Tue Mar 3 08:18:09 2015 From: mgflem <@t> gmail.com (Matthew Fleming) Date: Tue Mar 3 08:18:12 2015 Subject: [Histonet] Histotech coverage needed in Milwaukee, WI Message-ID: Help needed in covering a histotech's vacation in dermatopathology lab in downtown Milwaukee, WI. We need about 4 weeks' of coverage per year but one tech would not have to do all of it; even a week or 2 would be helpful. If interested, please respond to: Matthew Fleming, MD Fleming Dermatopathology 1661 N Water St, Ste 500 Milwaukee, WI 53202 mgflem@gmail.com From lcolbert <@t> pathmdlabs.com Tue Mar 3 09:45:55 2015 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Mar 3 09:46:00 2015 Subject: [Histonet] Masson trichrome and H and E In-Reply-To: References: Message-ID: <12ECD7346266D74691EC2BFC75285E4548B606FD@BFL323E10.pathmdlabs.local> Medical Chemical Corporation has a trichrome kit with 4 oz reagents for around $60.00. Laurie Colbert, HT (ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Tuesday, March 03, 2015 4:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Masson trichrome and H and E Hello! I am doing Masson trichrome manually (not with a machine) and I just found out the kit is $800!! I was thinking of buying the ingredients separately, but why the hell is it so expensive??? Also, the people I was going to borrow their reagents from said their aniline blue is not very good and I wanted to replace it. I only need about 250ml, what brand do you guys prefer? l know I can google this, but I want to know what you guys like and what works best. This is for mouse kidney paraffin sections, 4 to 5 microns. Another question, I did H and E and there is no eosin staining. I think the reagents are pretty old, so I thought that might be a problem. Also because my lab is cheap, they were reusing the xylenes and EtOH for both rehydrating and rehydrating. I told my boss this is probably not a good idea as the end steps will have stain in them. And I also think this is why it didn't work! The EtOH is also really old so who know is the 100% is actually even close to 100% any more. I'm buying new reagents, but if you guys think anything else would help, let me know. Also, shoutout to Ann, I know you're reading these!! Join the list!! Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sarah_Mack <@t> urmc.rochester.edu Tue Mar 3 10:04:37 2015 From: Sarah_Mack <@t> urmc.rochester.edu (Mack, Sarah) Date: Tue Mar 3 10:08:06 2015 Subject: [Histonet] New York State Histotechnological Society Annual Meeting Message-ID: The 2015 NYSHS Annual Meeting will be held on Saturday the 18th of April, 2015 at the Holiday Inn Express & Suites Latham in Latham New York. Check out our website for more information. http://www.nyhisto.com/ Sarah Mack From Ronald.Houston <@t> nationwidechildrens.org Tue Mar 3 10:08:03 2015 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Mar 3 10:08:10 2015 Subject: [Histonet] Lynch syndrome Message-ID: I know it is a long-shot, but does anyone know of a TMA made with known Lynch Syndrome cases? We have been asked to validate MSI antibodies for evaluating CMMRD (Constitutional Mismatch Repair Deficiency) in pediatric brain tumors and don't have access to Lynch Syndrome in our pediatric facility Any help appreciated Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From Terra.Wineman <@t> novusint.com Tue Mar 3 10:35:05 2015 From: Terra.Wineman <@t> novusint.com (Wineman, Terra) Date: Tue Mar 3 10:35:18 2015 Subject: [Histonet] Masson trichrome and H and E In-Reply-To: <12ECD7346266D74691EC2BFC75285E4548B606FD@BFL323E10.pathmdlabs.local> References: <12ECD7346266D74691EC2BFC75285E4548B606FD@BFL323E10.pathmdlabs.local> Message-ID: <1EB8F245A303564EADF12AC7022FA74DD76CE8D3@novus-ex01> I use the kit from Sigma-Aldrich (HT15 = $300). Terra Wineman, HTL (ASCP)CM Research Biologist, Nutritional Physiology 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Tuesday, March 03, 2015 9:46 AM To: Emily Brown; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Masson trichrome and H and E Medical Chemical Corporation has a trichrome kit with 4 oz reagents for around $60.00. Laurie Colbert, HT (ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Tuesday, March 03, 2015 4:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Masson trichrome and H and E Hello! I am doing Masson trichrome manually (not with a machine) and I just found out the kit is $800!! I was thinking of buying the ingredients separately, but why the hell is it so expensive??? Also, the people I was going to borrow their reagents from said their aniline blue is not very good and I wanted to replace it. I only need about 250ml, what brand do you guys prefer? l know I can google this, but I want to know what you guys like and what works best. This is for mouse kidney paraffin sections, 4 to 5 microns. Another question, I did H and E and there is no eosin staining. I think the reagents are pretty old, so I thought that might be a problem. Also because my lab is cheap, they were reusing the xylenes and EtOH for both rehydrating and rehydrating. I told my boss this is probably not a good idea as the end steps will have stain in them. And I also think this is why it didn't work! The EtOH is also really old so who know is the 100% is actually even close to 100% any more. I'm buying new reagents, but if you guys think anything else would help, let me know. Also, shoutout to Ann, I know you're reading these!! Join the list!! Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston <@t> nationwidechildrens.org Tue Mar 3 11:30:54 2015 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Mar 3 11:31:01 2015 Subject: [Histonet] HT vacancy Message-ID: We have an immediate opening for a registered HT. This would be a great opening for a newly registered tech as you would have the opportunity to learn and perform all aspects of histotechnology, from routine microtomy to electron microscopy, from special stains to IHC/ISH/IF and enzyme histochemistry. Please contact me for more information and go to our web-site to apply (https://careers.nationwidechildrens.org) and to see our incredible new facility (http://www.nationwidechildrens.org/) Come work for an organization where everything matters Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From jkiernan <@t> uwo.ca Tue Mar 3 11:34:33 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Mar 3 11:34:37 2015 Subject: [Histonet] Masson trichrome and H and E In-Reply-To: <7390b22c7f81.54f5f099@uwo.ca> References: <7300db053dff.54f5ec3d@uwo.ca> <7370b7cc6fc6.54f5ec7a@uwo.ca> <7370c7fc4576.54f5ecb8@uwo.ca> <73e09b1756d0.54f5ecf6@uwo.ca> <7310dae33589.54f5ed34@uwo.ca> <7310d0811920.54f5ed72@uwo.ca> <73e0a06a4e81.54f5edb0@uwo.ca> <7370daa646af.54f5edee@uwo.ca> <73c0a13b4533.54f5ee2c@uwo.ca> <7300aa807298.54f5ee6b@uwo.ca> <7370c1f8140e.54f5eea9@uwo.ca> <73909546622.54f5eee7@uwo.ca> <72e0df086066.54f5ef25@uwo.ca> <73709da434b4.54f5ef63@uwo.ca> <72d0c89c7093.54f5efa1@uwo.ca> <7370e4056cde.54f5efe0@uwo.ca> <7300d85079fa.54f5f01f@uwo.ca> <7390b22c7f81.54f5f099@uwo.ca> Message-ID: <7390afa3112a.54f5aa59@uwo.ca> If you can't get two colours with H&E, don't expect to get the colour scheme right with Masson's trichrome, which needs more skill. If you are hoping to show basement membranes in the kidney, you would do better to use a technically simpler staining method such as picro-sirius red or periodic acid-Schiff. If for some reason you really need three colours, a one-step trichrome such as Gomori's, Cason's or Gabe's might be the way to go rather than Masson's or one of the other multi-step trichromes. Remember that all trichrome methods are greatly influenced by the fixative. A post-fixation treatment of the sections, usually with picric acid, is needed for formaldehyde-fixed tissues. Some alternative post-fixation treatments were proposed by Yu & Chapman (2003) J. Histotechnol. 26(2): 131-134, but their coloured photos were not very convincing. Making up staining solutions in-house is always cheaper than buying pre-made solutions. John Kiernan London, Canada = = = On 03/03/15, Emily Brown wrote: > Hello! > > I am doing Masson trichrome manually (not with a machine) and I just found > out the kit is $800!! I was thinking of buying the ingredients separately, > but why the hell is it so expensive??? > Also, the people I was going to borrow their reagents from said their > aniline blue is not very good and I wanted to replace it. I only need > about 250ml, what brand do you guys prefer? > l know I can google this, but I want to know what you guys like and what > works best. This is for mouse kidney paraffin sections, 4 to 5 microns. > Another question, I did H and E and there is no eosin staining. I think > the reagents are pretty old, so I thought that might be a problem. Also > because my lab is cheap, they were reusing the xylenes and EtOH for both > rehydrating and rehydrating. I told my boss this is probably not a good > idea as the end steps will have stain in them. And I also think this is > why it didn't work! The EtOH is also really old so who know is the 100% is > actually even close to 100% any more. I'm buying new reagents, but if you > guys think anything else would help, let me know. > > Also, shoutout to Ann, I know you're reading these!! Join the list!! > > Emily > > > "By bitching and bitching and bitching, they could exhaust the drama of > their own horror stories. Grow bored. Only then could they accept a new > story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From CThornton <@t> dahlchase.com Tue Mar 3 12:09:51 2015 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Tue Mar 3 12:09:57 2015 Subject: [Histonet] IHC work flow Message-ID: I would love to hear about your IHC department or lab. We are looking to changing our work flow, and sometimes it's difficult to know if you are trying to perform miracles! These are the questions I have: 1. What is your volume of IHC slides/month? 2. How many antibodies do you offer? 3. What platforms do you use? Do you use platforms from multiple vendors? 4. What is your turn around time? Do you offer same day turn around, and under what conditions? 5. How many full time employees work in IHC? Do any work on a part time or rotating basis? 6. Is your IHC department part of general histology, or is it a separate department? 7. If you are continuous flow, how does this work? I will say that we use Ventana platforms (both Ultra and XT), so I would be interested in hearing about those who also use Ventana, but I would like to hear from users with other platforms as well. 8. If there are any other aspects you would like to mention, please add them! I appreciate the help on this. Every lab is a world unto itself, often seeing how other labs run their operations can open your eyes to additional possibilities. Thank you. Clare J. Thornton, HTL(ASCP)QIHC Lead Immunohistochemistry Technologist Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From gu.lang <@t> gmx.at Tue Mar 3 12:19:10 2015 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Mar 3 12:19:17 2015 Subject: AW: [Histonet] Masson trichrome and H and E In-Reply-To: <7390afa3112a.54f5aa59@uwo.ca> References: <7300db053dff.54f5ec3d@uwo.ca> <7370b7cc6fc6.54f5ec7a@uwo.ca> <7370c7fc4576.54f5ecb8@uwo.ca> <73e09b1756d0.54f5ecf6@uwo.ca> <7310dae33589.54f5ed34@uwo.ca> <7310d0811920.54f5ed72@uwo.ca> <73e0a06a4e81.54f5edb0@uwo.ca> <7370daa646af.54f5edee@uwo.ca> <73c0a13b4533.54f5ee2c@uwo.ca> <7300aa807298.54f5ee6b@uwo.ca> <7370c1f8140e.54f5eea9@uwo.ca> <73909546622.54f5eee7@uwo.ca> <72e0df086066.54f5ef25@uwo.ca> <73709da434b4.54f5ef63@uwo.ca> <72d0c89c7093.54f5efa1@uwo.ca> <7370e4056cde.54f5efe0@uwo.ca> <7300d85079fa.54f5f01f@uwo.ca> <7390b22c7f81.54f5f099@uwo.ca> <7390afa3112a.54f5aa59@uwo.ca> Message-ID: <000601d055de$8bd30ef0$a3792cd0$@gmx.at> Hi! For lacking Eosin-staining check the pH of the solution. It should be 4-5. With some drops of acetic acid you may recover the staining-action. Gudrun = = = On 03/03/15, Emily Brown wrote: > Hello! > > I am doing Masson trichrome manually (not with a machine) and I just > found out the kit is $800!! I was thinking of buying the ingredients > separately, but why the hell is it so expensive??? > Also, the people I was going to borrow their reagents from said their > aniline blue is not very good and I wanted to replace it. I only need > about 250ml, what brand do you guys prefer? > l know I can google this, but I want to know what you guys like and > what works best. This is for mouse kidney paraffin sections, 4 to 5 microns. > Another question, I did H and E and there is no eosin staining. I > think the reagents are pretty old, so I thought that might be a > problem. Also because my lab is cheap, they were reusing the xylenes > and EtOH for both rehydrating and rehydrating. I told my boss this is > probably not a good idea as the end steps will have stain in them. And > I also think this is why it didn't work! The EtOH is also really old > so who know is the 100% is actually even close to 100% any more. I'm > buying new reagents, but if you guys think anything else would help, let me know. > > Also, shoutout to Ann, I know you're reading these!! Join the list!! > > Emily > > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a > new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 3 12:28:11 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 3 12:28:18 2015 Subject: [Histonet] IHC work flow In-Reply-To: References: Message-ID: <99538258.2023944.1425407291682.JavaMail.yahoo@mail.yahoo.com> Clare: I think that in order to benefit the most from potential answers, you should have first offered the information from your lab for each of the questions, along with your TAT.By doing so?those willing to offer their information on the subject would have a better idea if your lab is performing at par with theirs.I think that in this way you will benefit the most.Ren? J.? On Tuesday, March 3, 2015 1:09 PM, Clare Thornton wrote: I would love to hear about your IHC department or lab.? We are looking to changing our work flow, and sometimes it's difficult to know if you are trying to perform miracles!? These are the questions I have: 1.? ? ? What is your volume of IHC slides/month? 2.? ? ? How many antibodies do you offer? 3.? ? ? What platforms do you use?? Do you use platforms from multiple vendors? 4.? ? ? What is your turn around time?? Do you offer same day turn around, and under what conditions? 5.? ? ? How many full time employees work in IHC?? Do any work on a part time or rotating basis? 6.? ? ? Is your IHC department part of general histology, or is it a separate department? 7.? ? ? If you are continuous flow, how does this work?? I will say that we use Ventana platforms (both Ultra and XT), so I would be interested in hearing about those who also use Ventana, but I would like to hear from users with other platforms as well. 8.? ? ? If there are any other aspects you would like to mention, please add them! I appreciate the help on this.? Every lab is a world unto itself, often seeing how other labs run their operations can open your eyes to additional possibilities.? Thank you. Clare J. Thornton, HTL(ASCP)QIHC Lead Immunohistochemistry Technologist Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 3 12:34:07 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 3 12:34:11 2015 Subject: AW: [Histonet] Masson trichrome and H and E In-Reply-To: <000601d055de$8bd30ef0$a3792cd0$@gmx.at> References: <000601d055de$8bd30ef0$a3792cd0$@gmx.at> Message-ID: <424186781.2004197.1425407647267.JavaMail.yahoo@mail.yahoo.com> Emely: To your initial e-mail the kit is so expensive because it incorporates?a salary component for the preparation of the solutions + any and all?enormous profit margin the manufacturer wants to add.I never/ever used kits, always used solutions prepared "from scratch" in my lab and the savings?were enormous.As to preferred dry dyes my best preferred are?Merk (Darmstad), Harleco and Sigma.Ren? J.? On Tuesday, March 3, 2015 1:19 PM, Gudrun Lang wrote: Hi! For lacking Eosin-staining check the pH of the solution. It should be 4-5. With some drops of acetic acid you may recover the staining-action. Gudrun = = = On 03/03/15, Emily Brown? wrote: > Hello! > > I am doing Masson trichrome manually (not with a machine) and I just > found out the kit is $800!! I was thinking of buying the ingredients > separately, but why the hell is it so expensive??? > Also, the people I was going to borrow their reagents from said their > aniline blue is not very good and I wanted to replace it. I only need > about 250ml, what brand do you guys prefer? > l know I can google this, but I want to know what you guys like and > what works best. This is for mouse kidney paraffin sections, 4 to 5 microns. > Another question, I did H and E and there is no eosin staining. I > think the reagents are pretty old, so I thought that might be a > problem. Also because my lab is cheap, they were reusing the xylenes > and EtOH for both rehydrating and rehydrating. I told my boss this is > probably not a good idea as the end steps will have stain in them. And > I also think this is why it didn't work! The EtOH is also really old > so who know is the 100% is actually even close to 100% any more. I'm > buying new reagents, but if you guys think anything else would help, let me know. > > Also, shoutout to Ann, I know you're reading these!! Join the list!! > > Emily > > > "By bitching and bitching and bitching, they could exhaust the drama > of their own horror stories. Grow bored. Only then could they accept a > new story for their lives. Move forward." > > -Chuck Palahniuk, "Haunted" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From taylor <@t> prometheushealthcare.com Tue Mar 3 13:25:21 2015 From: taylor <@t> prometheushealthcare.com (Taylor Rinaldi) Date: Tue Mar 3 13:20:21 2015 Subject: [Histonet] Histology Supervisor and Bench histotech opportunities in Alpharetta, Ga. Message-ID: <026401d055e7$ca7bed30$5f73c790$@prometheushealthcare.com> A growing Pathology laboratory in Alpharetta, Ga is looking to bring some fresh faces to their Histology department. They are currently looking to hire a new Histology Supervisor, and a bench Histotechnician. ASCP certification preferred. This is a permanent, full time opportunity. Please reach out to me for immediate consideration. Thanks so much! Taylor Rinaldi Nationwide Laboratory Recruiter Prometheus Healthcare Office (301) 693-9057 Taylor@prometheushealthcare.com From jqb7 <@t> cdc.gov Tue Mar 3 13:21:13 2015 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue Mar 3 13:21:35 2015 Subject: [Histonet] Dako Coverstainer Message-ID: <3B2CD438E1628A41BD687E98B963B78137EF15B0@EMBX-CLFT4.cdc.gov> Good afternoon all! I would like to hear back from those of you who have the Dako Coverstainer....pros and cons! Thanks much! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov From Lacie.Algeo <@t> providence.org Tue Mar 3 14:03:46 2015 From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A) Date: Tue Mar 3 14:04:22 2015 Subject: [Histonet] Frozen section calculations Message-ID: <24C4B3C167E5694887AB594C7602CE3A03BA8F5A@WN35104.or.providence.org> Hi All, When you receive multiple frozen sections on the same patient, does the pathologist call each one and then document the time for each one, or do they call in 'batches' and the time from receipt to call is divided by the total number of frozens done in that time frame for that patient. Or, does anyone have a different way that they do it? Is there any documentation recommending the process or recording frozen section times? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From anna.coffey <@t> nih.gov Tue Mar 3 14:35:20 2015 From: anna.coffey <@t> nih.gov (Coffey, Anna (NIH/NCI) [C]) Date: Tue Mar 3 14:36:38 2015 Subject: [Histonet] Microscopy training courses or certification programs Message-ID: <5C3E10119A1B824FBE92B08279F74A9101785B4F@msgb09.nih.gov> Hello Histonet, I am curious if anyone can recommend a good microscopy training course or certificate program? I'm looking primarily for training on light microscopes. Thanks, Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 301-846-1730 From cmconway <@t> usgs.gov Tue Mar 3 15:00:54 2015 From: cmconway <@t> usgs.gov (Conway, Carla) Date: Tue Mar 3 15:01:11 2015 Subject: [Histonet] Differentiating mycobacteria from Nocardia Message-ID: Hello, Could anyone provide a staining protocol that will differentiate mycobacteria from Nocardia in tissue sections? The bacteria were both acid-fast-positive by the Ziehl-Neelsen stain. Thank you! Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway@usgs.gov From hlukey <@t> msn.com Tue Mar 3 15:21:13 2015 From: hlukey <@t> msn.com (Hugh Luk) Date: Tue Mar 3 15:21:16 2015 Subject: [Histonet] Masson trichrome and H and E In-Reply-To: References: Message-ID: Hi Emily, My $0. 02 by saying it's a challenge for research groups, like ours, to keep quality stains that are not used everyday/week/month. I agree with your intuition. Buy or make new eosin. As for stocking eosin (or hematoxylin), I buy premade by a reputable vendor and always keep an eye on it getting weak or the dye adhering to the bottle. Oxidation hurts H&E stains, so I never return used dye to the "New" bottle once I have used them (I get rather oblique requests too). Alcohols are similar, as they always pull water out of the air, so my final dehydrating alcohols are always fresh. Xylenes are less fussy. For manual Masson trichrome, I use Newcomer Supply as I appreciate Marcia Walsh's humor, but most vendors are pretty good. 250 ml of aniline blue is $28.90. Works great. I don't know why your kit was $800, but I would guess it would be the phosphomolybdic/phoshotungstic acid. Her Masson kit (blue) is rather high too at $294 for the 250 ml. For me, aniline blue solution crashes first in the trichrome (other than the Weigerts). Have you any desire to make solution from powder? $27.10 for 25 g of certified dye? Just wondering. Shipping to my state adds $50-$100 to the price, so I think about stuff like this. Hugh Hawaii > ------------------------------ > Date: Tue, 3 Mar 2015 07:38:30 -0500 > From: Emily Brown > Subject: [Histonet] Masson trichrome and H and E > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Hello! > > I am doing Masson trichrome manually (not with a machine) and I just found > out the kit is $800!! I was thinking of buying the ingredients separately, > but why the hell is it so expensive??? > Also, the people I was going to borrow their reagents from said their > aniline blue is not very good and I wanted to replace it. I only need > about 250ml, what brand do you guys prefer? > l know I can google this, but I want to know what you guys like and what > works best. This is for mouse kidney paraffin sections, 4 to 5 microns. > Another question, I did H and E and there is no eosin staining. I think > the reagents are pretty old, so I thought that might be a problem. Also > because my lab is cheap, they were reusing the xylenes and EtOH for both > rehydrating and rehydrating. I told my boss this is probably not a good > idea as the end steps will have stain in them. And I also think this is > why it didn't work! The EtOH is also really old so who know is the 100% is > actually even close to 100% any more. I'm buying new reagents, but if you > guys think anything else would help, let me know. > > Also, shoutout to Ann, I know you're reading these!! Join the list!! > > Emily > > ------------------------------ From abtdhu <@t> gmail.com Tue Mar 3 18:17:46 2015 From: abtdhu <@t> gmail.com (abtdhu@gmail.com) Date: Tue Mar 3 18:17:49 2015 Subject: [Histonet] Alk pho staining on paraffin section Message-ID: Hi, I couldn't do on paraffin tissue after trying a few times. But works fine on MMA plastic section. If any one has a practical protocol, please share. Thanks in advance. Dorothy Hu Hello, I was wondering if anyone has had success in staining osteoblasts via alkaline phosphatase in decaled ffpe tissues? Currently using a sigma kit without success even with pre-incubating overnight with magnesium chloride. Thanks! Melanie -- Melanie Smith, MS University of Delaware melsmith@udel.edu From pablo.sanchez <@t> usc.es Wed Mar 4 02:32:43 2015 From: pablo.sanchez <@t> usc.es (Pablo Sanchez-Quinteiro) Date: Wed Mar 4 02:32:45 2015 Subject: [Histonet] Masson trichrome and H and E Message-ID: <0d1a8e$1of6qb@correo2bi.usc.es> >>At 19:19 03/03/2015, you wrote: >>Hi! >>For lacking Eosin-staining check the pH of the solution. It should be 4-5. >>Gudrun >>= = = Thanks for the tip! Nice to know it... after centuries doing stainings :-) Pablo On 03/03/15, Emily Brown wrote: > Hello! > > Another question, I did H and E and there is no eosin staining. I > think the reagents are pretty old, so I thought that might be a > problem. Also because my lab is cheap, they were reusing the xylenes > and EtOH for both rehydrating and rehydrating. I told my boss this is > probably not a good idea as the end steps will have stain in them. And > I also think this is why it didn't work! The EtOH is also really old > so who know is the 100% is actually even close to 100% any more. I'm > buying new reagents, but if you guys think anything else would help, let me know. From Valerie.Hannen <@t> parrishmed.com Wed Mar 4 08:24:37 2015 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Wed Mar 4 08:25:30 2015 Subject: [Histonet] IHC charges Message-ID: <450B7A81EDA0C54E97C53D60F00776C32337384221@isexstore03> Good Morning all.. We stopped doing IHC's some time ago and we send them out to a reference lab. If we send 4 blocks from 1 specimen, let's say part "A" and 2 blocks from specimen "B", from what I know, we can only charge for 1 block from each specimen per antibody. My question is this... Can the reference lab charge us for all 6 blocks?? Thanks in advance for your help! Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== From KSimeone <@t> leavittmgt.com Wed Mar 4 09:01:47 2015 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Wed Mar 4 09:01:55 2015 Subject: [Histonet] OPEN POSITIONS IN DELRAY, FL LAB Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C37C8E881@vm-email.leavittmgt.com> Hi Histonetters! We are looking for (2) full time licensed histotechs here in our very busy Delray Florida Dermatology Lab. These are permanent full time SECOND SHIFT (40 hours) positions with benefits (medical/401k/vacation) and competitive pay. THIS IS A DRUG FREE WORKPLACE. ONLY SERIOUS INQURIES, please read EVERY qualification desired. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengimann@leavittmgt.com if interested. *FIRST full time position Mon-Fri 12:30p-9p (general tech duties to include grossing, microtomy, embedding, etc) *MUST be licensed as a FL histotechnologist or technician *will train but you MUST be open and willing to learn and absorb the routine quickly *must be self motivated, reliable and a team player *SECOND full time position Mon-Fri 3p-11:30p (IMMUNOHISTOCHEMISTRY/SPECIALS department) *MUST be licensed as a FL HISTOTECHNOLOGIST *EXPERIENCE WITH IHC A MUST! Leica (BOND) and Roche/Ventana equipment experience preferred *MUST have at LEAST 2 years experience. Please DO NOT respond if no EXPERIENCE! *must be confidant, self motivated, reliable and a team player Kari M Simeone 561.819.6517 fax ksimeone@leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From Valerie.Hannen <@t> parrishmed.com Wed Mar 4 09:31:00 2015 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Wed Mar 4 09:58:30 2015 Subject: [Histonet] IHC charges In-Reply-To: References: <450B7A81EDA0C54E97C53D60F00776C32337384221@isexstore03> Message-ID: <450B7A81EDA0C54E97C53D60F00776C32337384222@isexstore03> Thanks Victoria. By any chance do you know the answer to my question about the way that the reference lab is charging us (they are only doing the technical component, our Pathologists are reading them)? Valerie Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com From: Victoria Baker [mailto:bakevictoria@gmail.com] Sent: Wednesday, March 04, 2015 10:20 AM To: Hannen, Valerie Subject: Re: [Histonet] IHC charges If you send 3 blocks from specimen A requesting the same antibody on each, then you can only bill for one ab. If you are ordering three different ab's on each one then it is one initial and two additional. You bill by the specimen. If your case has 3 separate specimens (ABC) then they are considered 3 separate sites. If you have multiple blocks per specimen it is still considered one site. The same thing applies to ISH/FISH. Each now has initial and additional each requires a separate CDM and CPT. Also there can only be one initial antibody everything else is an additional. So if you order 2 AB's today its 1 initial 1 additional. Tomorrow you order 2 more. They are both additional even though its a totally new order. This is what I've been experiencing, would love to hear what nightmares others are having. Vikki On Mar 4, 2015 9:25 AM, "Hannen, Valerie" > wrote: Good Morning all.. We stopped doing IHC's some time ago and we send them out to a reference lab. If we send 4 blocks from 1 specimen, let's say part "A" and 2 blocks from specimen "B", from what I know, we can only charge for 1 block from each specimen per antibody. My question is this... Can the reference lab charge us for all 6 blocks?? Thanks in advance for your help! Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com> www.parrishmed.com "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From tejohnson <@t> genoptix.com Wed Mar 4 10:53:50 2015 From: tejohnson <@t> genoptix.com (Teri Johnson) Date: Wed Mar 4 10:54:12 2015 Subject: [Histonet] HT grossing SOPs Message-ID: Dear colleagues, Is anyone available to share their grossing procedure that meets CAP/CLIA requirements for HTs doing grossing? I appreciate your input as always. PS. Please don't turn this into a "PA vs HT grossing" contest. BTDT Happy Wednesday! Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From Joyce.Weems <@t> emoryhealthcare.org Wed Mar 4 11:05:12 2015 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Wed Mar 4 11:05:29 2015 Subject: [Histonet] RE: IHC charges In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C32337384221@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C32337384221@isexstore03> Message-ID: You can only charge the patient as you describe, but I would think the reference lab would charge per their contract with you. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Wednesday, March 04, 2015 9:25 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC charges Good Morning all.. We stopped doing IHC's some time ago and we send them out to a reference lab. If we send 4 blocks from 1 specimen, let's say part "A" and 2 blocks from specimen "B", from what I know, we can only charge for 1 block from each specimen per antibody. My question is this... Can the reference lab charge us for all 6 blocks?? Thanks in advance for your help! Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Julia.Cates <@t> AHSS.ORG Wed Mar 4 12:53:13 2015 From: Julia.Cates <@t> AHSS.ORG (Cates, Julia) Date: Wed Mar 4 12:53:22 2015 Subject: [Histonet] Floor scraper Message-ID: Good Day Histonet! I am wondering if anyone can recommend a tool/product to clean paraffin off linoleum floors. We currently have a tool with a blade on it but it digs right into the flooring. Any and all suggestion will be helpful. Thanks, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. From DKBoyd <@t> chs.net Wed Mar 4 12:58:24 2015 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Wed Mar 4 12:59:48 2015 Subject: [Histonet] RE: Floor scraper In-Reply-To: References: Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9F0DA9B@TN001WEXMBX014.US.chs.net> We use a painter's spatula for sheet rock mud, taped to a broom handle. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Cates, Julia [Julia.Cates@AHSS.ORG] Sent: Wednesday, March 04, 2015 1:53 PM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Floor scraper Good Day Histonet! I am wondering if anyone can recommend a tool/product to clean paraffin off linoleum floors. We currently have a tool with a blade on it but it digs right into the flooring. Any and all suggestion will be helpful. Thanks, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From amurvosh <@t> advancederm.net Wed Mar 4 13:03:42 2015 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Wed Mar 4 13:03:52 2015 Subject: [Histonet] RE: Floor scraper In-Reply-To: References: Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7A6EEB1@Exchange.Advancederm.net> just a paint scraper at your local hardware store. Some people attach it to a broom handle so they don't have bend down. They may even have a long one. Anne ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Cates, Julia [Julia.Cates@AHSS.ORG] Sent: Wednesday, March 4, 2015 10:53 AM To: histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Floor scraper Good Day Histonet! I am wondering if anyone can recommend a tool/product to clean paraffin off linoleum floors. We currently have a tool with a blade on it but it digs right into the flooring. Any and all suggestion will be helpful. Thanks, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pasquale.deblasio <@t> isenet.it Wed Mar 4 13:31:04 2015 From: pasquale.deblasio <@t> isenet.it (Pasquale De Blasio) Date: Wed Mar 4 13:31:14 2015 Subject: [Histonet] Training Course on:Tissue Microarray (TMA) and Quantitative Pathology Image Analysis Message-ID: Training Course on: Tissue Microarray (TMA) and Quantitative Pathology Image Analysis Scheduled on March 26th 2015 from 10:00 a.m. To 5:00 p.m. The Ragon Institute of MGH, MIT and Harvard Address: 400 Technology Square, Room 890 Cambridge, MA 02139 Bring your own TMA slide for scanning. For Quantitative Analysis of your own TMA, For registration please contact Alex Barang (alex.barang@tissuegnostics.com). From jpiche <@t> wtbyhosp.org Wed Mar 4 13:38:41 2015 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Wed Mar 4 13:38:48 2015 Subject: [Histonet] p16 Message-ID: <631955447A364B45B9458D2905635110D7C18188@WIN08-MBX-01.wtbyhosp.org> Hi Everyone, I was wondering if people are using the p16 antibody and if so where are you getting it from? Thank you, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From mjones <@t> metropath.com Wed Mar 4 14:02:44 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Wed Mar 4 14:02:50 2015 Subject: [Histonet] p16 Message-ID: Ventana - we buy the large test dispenser. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 3/4/15, 12:38 PM, "Piche, Jessica" wrote: >Hi Everyone, > >I was wondering if people are using the p16 antibody and if so where are >you getting it from? > >Thank you, > >Jessica Piche, HT(ASCP) >Waterbury Hospital > > > >CONFIDENTIALITY NOTICE: This email and any attachments contain >confidential information that is legally privileged. This information is >intended only for the use of the individual or entity named above. The >authorized recipient of this information is prohibited from disclosing >this information to any other party unless required to do so by law or >regulation. If you are not the intended recipient, you are hereby >notified that any disclosure, copying, distribution or action taken in >reliance on the contents of these documents is strictly prohibited. If >you have received this information in error, please notify the sender >immediately and delete these documents. Copyright (c) Waterbury Hospital >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ruio7 <@t> hotmail.com Wed Mar 4 14:20:25 2015 From: ruio7 <@t> hotmail.com (Rui TAHARA) Date: Wed Mar 4 14:20:29 2015 Subject: [Histonet] decalcification after dehydration Message-ID: I have an adult bird skull that fixed with formalin and then has been stored in 70% ethanol. I have seen the post that the sample stored in 70% ethanol can be walking back through to series of ethanol to water and can be decalcified if it needs to be. I am wondering if anybody has done this and there is any side effects from decalcification after going through dehydration and rehydration of a sample compared to a general straight forward protocol from decalcification to dehydration? Thank you, rui From jm <@t> personifysearch.com Wed Mar 4 14:54:05 2015 From: jm <@t> personifysearch.com (Justin McDonald) Date: Wed Mar 4 14:54:11 2015 Subject: [Histonet] Histology Technician Opening Message-ID: <5bba2efde78782fa3223922cf5ca80e6@mail.gmail.com> Hello Histonet! I am reaching out on behalf of my retained client, a worldwide leader in immunohistochemistry. This company is headquartered in Northern IL, about 45 minutes north of Chicago. This company is looking for someone who has worked as a histology technician, ideally in a clinical or hospital laboratory. This position will be working in the in-house lab out of the company's headquarters, and will offer a strong base salary and benefits. If this seems like something you would be interested in please respond back to me directly with some times we can discuss this in more detail. If you could also provide the best number I could use to reach you and your credentials that would be great! Please still reach out even if you are not interested at this time but would still like to network for future opportunities. Thanks! Justin --------------------------------------------------------------------------------------------------------------------------- Justin McDonald Talent Management Executive Personify 5020 Weston Parkway Suite 315 Cary, North Carolina 27513 (Toll Free) 800.875.6188 x 129 (Direct) 919.460.4466 www.personifysearch.com From craigak12 <@t> gmail.com Wed Mar 4 15:04:52 2015 From: craigak12 <@t> gmail.com (Jb) Date: Wed Mar 4 15:05:00 2015 Subject: [Histonet] Controls: Message-ID: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone From shklei <@t> bellin.org Wed Mar 4 15:12:15 2015 From: shklei <@t> bellin.org (Shannon Kleiner) Date: Wed Mar 4 15:12:25 2015 Subject: [Histonet] p16 In-Reply-To: <631955447A364B45B9458D2905635110D7C18188@WIN08-MBX-01.wtbyhosp.org> References: <631955447A364B45B9458D2905635110D7C18188@WIN08-MBX-01.wtbyhosp.org> Message-ID: <54F720CF020000810000B31E@mail.bellin.org> Hello Jessica, We use Cintec p16 on our Bond Max. Here is a link you may find useful.... http://www.ventana.com/cervical Shannon Logan HTL (ASCP) Green Bay, WI >>> "Piche, Jessica" 3/4/2015 1:38 PM >>> Hi Everyone, I was wondering if people are using the p16 antibody and if so where are you getting it from? Thank you, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun <@t> hhchealth.org Wed Mar 4 16:56:22 2015 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Wed Mar 4 16:56:29 2015 Subject: [Histonet] Pathology software Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E3E1018AE@HHCEXCHMB03.hhcsystem.org> Can anyone recommend an inexpensive pathology software package for a small Dermatopathology laboratory? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From liz <@t> premierlab.com Wed Mar 4 17:08:24 2015 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Mar 4 17:08:29 2015 Subject: [Histonet] RE: Pathology software In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E3E1018AE@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E3E1018AE@HHCEXCHMB03.hhcsystem.org> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79F17D8D@SBS2K8.premierlab.local> Richard There is a company that designs barcoding software for smaller histology labs it's called Cerebrum The contact information that I have on file is this: Gregg Lahti gregg@cerebrumcorp.com http://cerebrumcorp.com/ Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Wednesday, March 04, 2015 3:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology software Can anyone recommend an inexpensive pathology software package for a small Dermatopathology laboratory? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis <@t> bresnan.net Wed Mar 4 17:25:28 2015 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Mar 4 17:25:47 2015 Subject: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification Message-ID: <000601d056d2$8050bc40$80f234c0$@bresnan.net> You wrote: I have an adult bird skull that fixed with formalin and then has been stored in 70% ethanol. I have seen the post that the sample stored in 70% ethanol can be walking back through to series of ethanol to water and can be decalcified if it needs to be. I am wondering if anybody has done this and there is any side effects from decalcification after going through dehydration and rehydration of a sample compared to a general straight forward protocol from decalcification to dehydration? **************************************************************************** **************************************************************************** ******************************** I have, in the past, when a weekend arrive, I interrupted acid bone decalcification by removing it from acid decalcifier, a quick water rinse and immersed into 70% alcohol before returning bone to fresh acid decalcifier the next working day. The bones always decalcified without problems but I am sure the decalcification took longer since partially decalcified bone had to rehydrate. I later learned more about dipolar (hope I said that correctly) alcohol slowing and/or stopping ionization of calcium and ceased using 70% alcohol to interrupt acid decalcification. I now use NBF to interrupt decalcification. Interestingly, I learned the alcohol technique from the AFIP bone pathology lab. Alcohol is put into Perenyi's nitric acid decalcifying solutions to slow down or control very rapid nitric acid decalcification. You did not say how big the bird skull was? I suggest immersing the skull back into NBF to let it totally rehydrate for several days (depending on skull size and if the brain is present). I suggest changing NBF if you rehydrate longer than a day. You don't need to go back through an alcohol gradient since many processing schedules have tissue samples going from NBF directly into 70%. If you leave residual alcohol in the bones, the acid decalcification could be slower and hopefully not retarded in any way. It certainly is worth a try. Good luck. Gayle M. Callis HTL/HT/MT(ASCP) From ruio7 <@t> hotmail.com Wed Mar 4 17:38:56 2015 From: ruio7 <@t> hotmail.com (Rui TAHARA) Date: Wed Mar 4 17:39:00 2015 Subject: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification In-Reply-To: <000601d056d2$8050bc40$80f234c0$@bresnan.net> References: <000601d056d2$8050bc40$80f234c0$@bresnan.net> Message-ID: Thank you for helpful suggestions. I have further questions. Yes, I have a bird head (probably 1 cm X 1 cm ) stored in 70 % ethanol. But i have a similar size bird head fixed in 3.7% formalin for over night and am actually processing the head to store in 70% ethanol since my lab is just ordering the decalcifying solution. I need to decalcify this sample later. But i am wondering if it is better to keep the sample in formalin for a week or so till i get the decalcification solution or i should store it in 70 % ethanol and then fix it for a few days again later? I am afraid that longer fixative time would affect the sample somehow (e.g. the sample become too rigid?) Thank you, rui > From: gayle.callis@bresnan.net > To: histonet@lists.utsouthwestern.edu > Date: Wed, 4 Mar 2015 16:25:28 -0700 > Subject: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification > > You wrote: > > > > I have an adult bird skull that fixed with formalin and then has been stored > in 70% ethanol. > > I have seen the post that the sample stored in 70% ethanol can be walking > back through to series of ethanol to water and can be decalcified if it > needs to be. > > > > I am wondering if anybody has done this and there is any side effects from > decalcification after going through dehydration and rehydration of a sample > compared to a general straight forward protocol from decalcification to > dehydration? > > **************************************************************************** > **************************************************************************** > ******************************** > > > > I have, in the past, when a weekend arrive, I interrupted acid bone > decalcification by removing it from acid decalcifier, a quick water rinse > and immersed into 70% alcohol before returning bone to fresh acid > decalcifier the next working day. The bones always decalcified without > problems but I am sure the decalcification took longer since partially > decalcified bone had to rehydrate. I later learned more about dipolar (hope > I said that correctly) alcohol slowing and/or stopping ionization of calcium > and ceased using 70% alcohol to interrupt acid decalcification. I now use > NBF to interrupt decalcification. Interestingly, I learned the alcohol > technique from the AFIP bone pathology lab. > > > > Alcohol is put into Perenyi's nitric acid decalcifying solutions to slow > down or control very rapid nitric acid decalcification. > > > > You did not say how big the bird skull was? I suggest immersing the skull > back into NBF to let it totally rehydrate for several days (depending on > skull size and if the brain is present). I suggest changing NBF if you > rehydrate longer than a day. You don't need to go back through an alcohol > gradient since many processing schedules have tissue samples going from NBF > directly into 70%. If you leave residual alcohol in the bones, the acid > decalcification could be slower and hopefully not retarded in any way. > > > > > It certainly is worth a try. Good luck. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jonathan.Arzt <@t> ARS.USDA.GOV Thu Mar 5 04:27:11 2015 From: Jonathan.Arzt <@t> ARS.USDA.GOV (Arzt, Jonathan) Date: Thu Mar 5 04:27:30 2015 Subject: [Histonet] HT Job Opportunity at Plum Island Message-ID: <7213AC15544DE945843D821E1769E38A06A1CC05@001FSN2MPN1-053.001f.mgd2.msft.net> Immediately available and open for application for just 2 weeks, is a full-time, permanent, federal histotchnologist position with the Foreign Animal Disease Research Unit at the Plum Island Animal Disease Center. Job Title: Biologist (Histotechnologist) Department: Department Of Agriculture Agency: Agricultural Research Service Job Announcement Number: ARS-S15E-0045 SALARY RANGE: $44,617.00 to $85,841.00 / Per Year See the full posting and application details here: https://www.usajobs.gov/GetJob/ViewDetails/396084800 ******************************************************** Jonathan Arzt DVM, MPVM, PhD, DACVP Veterinary Medical Officer (Pathology) Foreign Animal Disease Research Unit USDA/ARS Plum Island Animal Disease Center P.O. Box 848, Greenport NY 11944 631-323-3336(ph) 631-323-3006(fax) Jonathan.Arzt@ARS.USDA.GOV ******************************************************** This electronic message contains information generated by the USDA solely for the intended recipients. Any unauthorized interception of this message or the use or disclosure of the information it contains may violate the law and subject the violator to civil or criminal penalties. If you believe you have received this message in error, please notify the sender and delete the email immediately. From gagnone <@t> KGH.KARI.NET Thu Mar 5 08:03:01 2015 From: gagnone <@t> KGH.KARI.NET (Gagnon, Eric) Date: Thu Mar 5 08:03:13 2015 Subject: [Histonet] Fixation before Decalcification? Message-ID: <5F06C3AD0B27264CA20CFA986C87882E0107AAA400@EXCHANGEPV3.KGH.ON.CA> What are you placing tissue blocks in before decalcification? We place blocks for decal in a jar of Bouin's Fixative from any time on Day 1, for specimens received in 10% NBF, until decalcification starts on Day 2. In other words, we gross specimens received in 10% NBF, then fix overnight in Bouin's, then begin decalcification the next morning. Two reasons for this - a decades-old reference, plus "we've always done it that way". We manually transfer the Bouin's-fixed blocks through graded alcohols to water, then use RDO as our decalcifying solution, then place in 10% NBF until blocks loaded on processor. But, going with the current "everything is on the table" approach in our laboratory, we are looking closely at pre-decalcification fixation of Bone Marrow Biopsies in 10% NBF (previously Dacie's then AZF) and we are pulling back the focus to treating larger decal blocks i.e. femoral head sections and other bone specimens, in the same way. Interested in your thoughts on pre-decalcification fixation.... Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada From relia1 <@t> earthlink.net Thu Mar 5 08:22:06 2015 From: relia1 <@t> earthlink.net (Pam Barker) Date: Thu Mar 5 08:22:11 2015 Subject: [Histonet] RELIA HOT Job Alert!! Histotechnologist needed for BRAND NEW LAB in Milwaukee!! A RELIA EXCLUSIVE Message-ID: <007b01d0574f$c2650e60$472f2b20$@earthlink.net> Hi Histonetters!! I hope everyone is having a great day and gearing up to celebrate Histotechnology Professionals Day next week. If you are still in the planning phase here is a link to some great ideas to mark the day courtesy of the NSH - http://www.nsh.org/content/histotechnology-professionals-day I also want to tell you about a new opportunity that we are working on. RELIA has been retained exclusively by a brand new lab in Milwaukee that is in need of a histotechnologist. Here is the info: Histotechnologist - Brand new Lab in Milwaukee! RELIA Solutions the nation's only recruiting firm specializing in the permanent placement of histology professionals has been retained by a BRAND NEW LAB located in Milwaukee to aid in their search for a histotechnologist. This is a RELIA exclusive. My client needs a strong ASCP certified and CLIAA qualified histotech who has experience with grossing, and routine histology in a dermpath environment. Knowledge of Mohs is a plus. My client offers excellent compensation and benefits, a great team to work with and limitless opportunities. This is a full time day shift position. If this job is for you we can make it happen and fast!! Our client is ready to hire today!! For more information pleases contact Pam Barker at relia1@earthlink.net or toll free at 866-607-3542. RELIA Solutions is the nation's ONLY recruiting firm specializing in the nationwide permanent placement of histology professionals. To sign up for our free histology careers bulletin please send an e-mail to relia1@earthlink.net and include subscribe in the subject line. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From JRobinson <@t> pathology-associates.com Thu Mar 5 09:08:04 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Thu Mar 5 09:08:13 2015 Subject: [Histonet] RE: Ventana Ultra CD10 In-Reply-To: <3CEB8EBCF9C7A648B9694B5696462A7172642055@NT-EX2.wvuhs.com> References: <3CEB8EBCF9C7A648B9694B5696462A7172642055@NT-EX2.wvuhs.com> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8CDAD@PAEXCH1.PathologyAssociates.local> Hi Beth Ann- I have also had problems with the Ventana CD10 after years of using it successfully. I don't know if they changed something or what but it just dropped off all of a sudden. I worked with one of their applications specialists on it for some time (different lot #'s, protocol changes, etc.) but was still unsuccessful in getting the problem resolved. I also have a Leica Bond on which I also run CD10. I ended up buying the Leica CD10 and putting it in a prep kit on the Ultra. I have to use the amplifier with it and the protocol is a little long (3 1/2 hours I think) but it works OK. I also have a Ventana Benchmark XT but I was unsuccessful in getting the Leica CD10 optimized on that instrument (I use the iView detection kit on that so I think that is the main problem). I had a similar problem with the Ventana CD30 and had to switch to the CellMarque CD30 in that case but I was able to get that one optimized and validated on both the Ultra and the XT. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of O'neil, Beth Sent: Thursday, February 26, 2015 1:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Ultra CD10 We are in the last stages of contract negotiations for purchasing two Ventana Benchmark Ultras. During this time period we have been optimizing our current inventory of approximately 110 antibodies and validating before our current instrumentation is removed within the next two weeks. My Ventana application specialist is unable to successfully optimize CD10 (Ventana clone SP67) on the Ventana Benchmark Ultra. He had me send slides to their applications/troubleshooting lab but they told us they won't start working on it until next week and then it would take about a week for them to try and optimize it. We are in an urgent rush to get this antibody optimized and validated within the next two weeks since it is heavily requested by our Hemepaths. Would anyone be willing to share their Ultra protocols with me? Has anyone had similar experiences with Ventana being unsuccessful and having to send their slides to their applications lab for work up? Thank you for your help. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC oneilb@wvuhealthcare.com Histology Supervisor, Technical Specialist Lab: 304 - 293 - 6014 Office: 304 - 293 - 7629 ----------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From JRobinson <@t> pathology-associates.com Thu Mar 5 09:21:20 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Thu Mar 5 09:21:33 2015 Subject: [Histonet] Controls: In-Reply-To: References: Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8CDE3@PAEXCH1.PathologyAssociates.local> We have successfully used hamburger meat to make Gram controls. As far as GMS controls go, we ran across a post from someone smearing cream cheese onto lung tissue and letting it sit for a couple of days, fixed and processed it and were able to demonstrate Aspergillus by GMS. My fungus control stocks are low so I was actually planning to try this with some beef lung. I haven't heard of the Slim Jim method before. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Wednesday, March 04, 2015 1:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From b-frederick <@t> northwestern.edu Thu Mar 5 09:43:53 2015 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Mar 5 09:43:58 2015 Subject: [Histonet] Controls: In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D8CDE3@PAEXCH1.PathologyAssociates.local> References: <204A03EB5A7F0A4BB1EEDD52A963829C16D8CDE3@PAEXCH1.PathologyAssociates.local> Message-ID: There was actually an article written in The Journal of Histotechnology a few years back on this and it was, I f I recall, using a hot dog to create the gram control. Might be in the archives on nsh.org. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Thursday, March 05, 2015 9:21 AM To: Jb; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls: We have successfully used hamburger meat to make Gram controls. As far as GMS controls go, we ran across a post from someone smearing cream cheese onto lung tissue and letting it sit for a couple of days, fixed and processed it and were able to demonstrate Aspergillus by GMS. My fungus control stocks are low so I was actually planning to try this with some beef lung. I haven't heard of the Slim Jim method before. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Wednesday, March 04, 2015 1:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun <@t> hhchealth.org Thu Mar 5 09:43:55 2015 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Thu Mar 5 09:44:02 2015 Subject: [Histonet] Chlamydia control tissue Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E3E101A0F@HHCEXCHMB03.hhcsystem.org> I have asked this in the past without any success, but I need to ask again, "Does anyone have a control tissue for Chlamydia" that they would be willing to share? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From abuchiane <@t> bmhvt.org Thu Mar 5 10:08:11 2015 From: abuchiane <@t> bmhvt.org (Anita Buchiane) Date: Thu Mar 5 10:08:21 2015 Subject: [Histonet] Prostate Biopsy code Message-ID: <4034E71604330C4B8E10D1538DFB2455EC6DD7FD@BMHEXCH02.bmhvt.org> I know the CMS (Medicare) code for 10-20 prostate biopsy specimens is G0416 but what is the Cpt (non-Medicare) code? _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ From LRaff <@t> uropartners.com Thu Mar 5 10:11:08 2015 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Thu Mar 5 10:11:22 2015 Subject: [Histonet] Prostate Biopsy code In-Reply-To: <4034E71604330C4B8E10D1538DFB2455EC6DD7FD@BMHEXCH02.bmhvt.org> References: <4034E71604330C4B8E10D1538DFB2455EC6DD7FD@BMHEXCH02.bmhvt.org> Message-ID: <0A05EC63892F834395DCC6047CAD0C5B9B62B8@UPHQMSX01.uropartners.local> For commercial, each individually submitted prostate biopsy is coded as 88305. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anita Buchiane Sent: Thursday, March 05, 2015 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prostate Biopsy code I know the CMS (Medicare) code for 10-20 prostate biopsy specimens is G0416 but what is the Cpt (non-Medicare) code? _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> uropartners.com Thu Mar 5 10:15:23 2015 From: LRaff <@t> uropartners.com (Lester Raff MD) Date: Thu Mar 5 10:15:39 2015 Subject: [Histonet] Prostate Biopsy code--One more point In-Reply-To: <0A05EC63892F834395DCC6047CAD0C5B9B62B8@UPHQMSX01.uropartners.local> References: <4034E71604330C4B8E10D1538DFB2455EC6DD7FD@BMHEXCH02.bmhvt.org> <0A05EC63892F834395DCC6047CAD0C5B9B62B8@UPHQMSX01.uropartners.local> Message-ID: <0A05EC63892F834395DCC6047CAD0C5B9B62B9@UPHQMSX01.uropartners.local> Also, as of 2015, the G0416 code is used for ANY NUMBER of prostate biopsies for Medicare, not just 10-20. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Thursday, March 05, 2015 10:11 AM To: Anita Buchiane; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Prostate Biopsy code For commercial, each individually submitted prostate biopsy is coded as 88305. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anita Buchiane Sent: Thursday, March 05, 2015 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prostate Biopsy code I know the CMS (Medicare) code for 10-20 prostate biopsy specimens is G0416 but what is the Cpt (non-Medicare) code? _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michael.Baker <@t> cchmc.org Thu Mar 5 10:40:44 2015 From: Michael.Baker <@t> cchmc.org (Baker, Michael) Date: Thu Mar 5 10:40:50 2015 Subject: [Histonet] Re: Controls In-Reply-To: <07.45.01004.78B78F45@mx7.cchmc.org> References: <07.45.01004.78B78F45@mx7.cchmc.org> Message-ID: Sounds like a plant spreading anti-Slim Jim propaganda. What?s next? Donut holes? ---- Michael Baker, M.D. CCHMC Pathology > On Mar 5, 2015, at 10:51 AM, histonet-request@lists.utsouthwestern.edu wrote: > > ------------------------------ > > Message: 9 > Date: Wed, 4 Mar 2015 14:04:52 -0700 > From: Jb > Subject: [Histonet] Controls: > To: Histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=us-ascii > > Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? > > Thank you, > > Sent from my iPhone > > > ------------------------------ From sforeman <@t> labpath.com Thu Mar 5 10:52:49 2015 From: sforeman <@t> labpath.com (Susan Foreman) Date: Thu Mar 5 10:55:48 2015 Subject: [Histonet] Controls: In-Reply-To: References: <204A03EB5A7F0A4BB1EEDD52A963829C16D8CDE3@PAEXCH1.PathologyAssociates.local> Message-ID: <00ee01d05764$d0471590$70d540b0$@labpath.com> Slim Jims do work for use as Gram Controls -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, March 05, 2015 10:44 AM To: Jeffrey Robinson; Jb; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls: There was actually an article written in The Journal of Histotechnology a few years back on this and it was, I f I recall, using a hot dog to create the gram control. Might be in the archives on nsh.org. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Thursday, March 05, 2015 9:21 AM To: Jb; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls: We have successfully used hamburger meat to make Gram controls. As far as GMS controls go, we ran across a post from someone smearing cream cheese onto lung tissue and letting it sit for a couple of days, fixed and processed it and were able to demonstrate Aspergillus by GMS. My fungus control stocks are low so I was actually planning to try this with some beef lung. I haven't heard of the Slim Jim method before. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Wednesday, March 04, 2015 1:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmire <@t> cvpath.org Thu Mar 5 10:58:48 2015 From: rmire <@t> cvpath.org (Ronda Mire) Date: Thu Mar 5 10:58:57 2015 Subject: [Histonet] Controls In-Reply-To: References: <07.45.01004.78B78F45@mx7.cchmc.org> Message-ID: Slim Jim will work as a control for gram stain. Can you believe people eat this crap? > On Mar 5, 2015, at 11:40 AM, Baker, Michael wrote: > > Sounds like a plant spreading anti-Slim Jim propaganda. What?s next? Donut holes? > > ---- > Michael Baker, M.D. > CCHMC Pathology > >> On Mar 5, 2015, at 10:51 AM, histonet-request@lists.utsouthwestern.edu wrote: >> >> ------------------------------ >> >> Message: 9 >> Date: Wed, 4 Mar 2015 14:04:52 -0700 >> From: Jb >> Subject: [Histonet] Controls: >> To: Histonet@lists.utsouthwestern.edu >> Message-ID: >> Content-Type: text/plain; charset=us-ascii >> >> Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? >> >> Thank you, >> >> Sent from my iPhone >> >> >> ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shklei <@t> bellin.org Thu Mar 5 11:06:58 2015 From: shklei <@t> bellin.org (Shannon Kleiner) Date: Thu Mar 5 11:07:06 2015 Subject: [Histonet] TRAP control Message-ID: <54F838D2020000810000B346@mail.bellin.org> Hello all, IHC question: What control can be used for the IHC Trap other than Hairy Cell? Any help is appreciated! From Timothy.Morken <@t> ucsf.edu Thu Mar 5 11:08:17 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Thu Mar 5 11:10:21 2015 Subject: [Histonet] Controls In-Reply-To: References: <07.45.01004.78B78F45@mx7.cchmc.org> Message-ID: <761E2B5697F795489C8710BCC72141FF367F8BF9@ex07.net.ucsf.edu> I agree it sounds bad, But the reality is that all food products have loads of dead or low count living bacteria. For example, milk is pasteurized to kill them, but they are still in the milk. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ronda Mire Sent: Thursday, March 05, 2015 8:59 AM To: Baker, Michael Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Controls Slim Jim will work as a control for gram stain. Can you believe people eat this crap? > On Mar 5, 2015, at 11:40 AM, Baker, Michael wrote: > > Sounds like a plant spreading anti-Slim Jim propaganda. What?s next? Donut holes? > > ---- > Michael Baker, M.D. > CCHMC Pathology > >> On Mar 5, 2015, at 10:51 AM, histonet-request@lists.utsouthwestern.edu wrote: >> >> ------------------------------ >> >> Message: 9 >> Date: Wed, 4 Mar 2015 14:04:52 -0700 >> From: Jb >> Subject: [Histonet] Controls: >> To: Histonet@lists.utsouthwestern.edu >> Message-ID: >> Content-Type: text/plain; charset=us-ascii >> >> Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? >> >> Thank you, >> >> Sent from my iPhone >> >> >> ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garreyf <@t> gmail.com Thu Mar 5 11:10:22 2015 From: garreyf <@t> gmail.com (Garreyf) Date: Thu Mar 5 11:10:26 2015 Subject: [Histonet] Controls In-Reply-To: References: <07.45.01004.78B78F45@mx7.cchmc.org> Message-ID: <18A4B0C1-17BF-481B-A279-DCA9FFC1AD17@gmail.com> I am in need of both a gram and fungal control and will have to try the slim Jim, hamburger and hotdog tricks. Thanks. If they work I will post A link to pictures. Sent from my iPhone > On Mar 5, 2015, at 11:58 AM, Ronda Mire wrote: > > Slim Jim will work as a control for gram stain. Can you believe people eat this crap? >> On Mar 5, 2015, at 11:40 AM, Baker, Michael wrote: >> >> Sounds like a plant spreading anti-Slim Jim propaganda. What?s next? Donut holes? >> >> ---- >> Michael Baker, M.D. >> CCHMC Pathology >> >>> On Mar 5, 2015, at 10:51 AM, histonet-request@lists.utsouthwestern.edu wrote: >>> >>> ------------------------------ >>> >>> Message: 9 >>> Date: Wed, 4 Mar 2015 14:04:52 -0700 >>> From: Jb >>> Subject: [Histonet] Controls: >>> To: Histonet@lists.utsouthwestern.edu >>> Message-ID: >>> Content-Type: text/plain; charset=us-ascii >>> >>> Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? >>> >>> Thank you, >>> >>> Sent from my iPhone >>> >>> >>> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Thu Mar 5 11:57:42 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Thu Mar 5 11:57:51 2015 Subject: [Histonet] Controls In-Reply-To: <761E2B5697F795489C8710BCC72141FF367F8BF9@ex07.net.ucsf.edu> References: <07.45.01004.78B78F45@mx7.cchmc.org> <761E2B5697F795489C8710BCC72141FF367F8BF9@ex07.net.ucsf.edu> Message-ID: Blech!!! Makes me want to run out and eat a hotdog!!! Ugh. . . (Good to know for controls though) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 3/5/15, 10:08 AM, "Morken, Timothy" wrote: >I agree it sounds bad, But the reality is that all food products have >loads of dead or low count living bacteria. For example, milk is >pasteurized to kill them, but they are still in the milk. > > >Tim Morken > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ronda Mire >Sent: Thursday, March 05, 2015 8:59 AM >To: Baker, Michael >Cc: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Controls > >Slim Jim will work as a control for gram stain. Can you believe people >eat this crap? >> On Mar 5, 2015, at 11:40 AM, Baker, Michael >>wrote: >> >> Sounds like a plant spreading anti-Slim Jim propaganda. What?s next? >>Donut holes? >> >> ---- >> Michael Baker, M.D. >> CCHMC Pathology >> >>> On Mar 5, 2015, at 10:51 AM, histonet-request@lists.utsouthwestern.edu >>>wrote: >>> >>> ------------------------------ >>> >>> Message: 9 >>> Date: Wed, 4 Mar 2015 14:04:52 -0700 >>> From: Jb >>> Subject: [Histonet] Controls: >>> To: Histonet@lists.utsouthwestern.edu >>> Message-ID: >>> Content-Type: text/plain; charset=us-ascii >>> >>> Off the wall question, I have been told that slim jims (pepperoni >>>stick) at the gas station can be processed and used as good gram >>>controls. Has anyone done this and do they work for GMS also? >>> >>> Thank you, >>> >>> Sent from my iPhone >>> >>> >>> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson <@t> gnf.org Thu Mar 5 12:01:14 2015 From: JWatson <@t> gnf.org (James Watson) Date: Thu Mar 5 12:01:19 2015 Subject: [Histonet] Controls: In-Reply-To: References: Message-ID: Years ago (70's and 80's) Slim Jims were used as Gram pos & neg controls in all the labs at AFIP. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Wednesday, March 04, 2015 1:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rajanbhatt <@t> gmail.com Thu Mar 5 12:02:34 2015 From: rajanbhatt <@t> gmail.com (Rajan Bhatt) Date: Thu Mar 5 12:02:45 2015 Subject: [Histonet] Controls: In-Reply-To: References: Message-ID: <005E20A7-CA7E-45A5-8DCE-447DA61EB225@gmail.com> unsubscribe On Mar 5, 2015, at 11:01 AM, James Watson wrote: > Years ago (70's and 80's) Slim Jims were used as Gram pos & neg controls in all the labs at AFIP. > > James Watson HT ASCP > GNF Genomics Institute of the Novartis Research Foundation > Scientific Technical Leader II, Histology > Tel 858-332-4647 > Fax 858-812-1915 > jwatson@gnf.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb > Sent: Wednesday, March 04, 2015 1:05 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Controls: > > Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? > > Thank you, > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brendal.finlay <@t> medicalcenterclinic.com Thu Mar 5 12:01:47 2015 From: brendal.finlay <@t> medicalcenterclinic.com (Brendal Finlay) Date: Thu Mar 5 12:03:35 2015 Subject: [Histonet] Controls References: <07.45.01004.78B78F45@mx7.cchmc.org> Message-ID: Ok, I'm curious. Would any of these work for an AFB control? ? Brendal C. Finlay, HT (ASCP) Senior Histologist Medical Center Clinic 8333 North Davis Highway Pensacola, FL 32514 Phone 850.474.8581 Fax 850.474.8584? -----Original Message----- From: Garreyf To: "Ronda Mire" Cc: histonet@lists.utsouthwestern.edu, "Baker, Michael" Date: 03/05/2015 11:10 Subject: Re: [Histonet] Controls I am in need of both a gram and fungal control and will have to try the slim Jim, hamburger and hotdog tricks. Thanks. If they work I will post A link to pictures. Sent from my iPhone > On Mar 5, 2015, at 11:58 AM, Ronda Mire wrote: > > Slim Jim will work as a control for gram stain. ?Can you believe people eat this crap? >> On Mar 5, 2015, at 11:40 AM, Baker, Michael wrote: >> >> Sounds like a plant spreading anti-Slim Jim propaganda. ?What?s next? ?Donut holes? >> >> ---- >> Michael Baker, M.D. >> CCHMC Pathology >> >>> On Mar 5, 2015, at 10:51 AM, histonet-request@lists.utsouthwestern.edu wrote: >>> >>> ------------------------------ >>> >>> Message: 9 >>> Date: Wed, 4 Mar 2015 14:04:52 -0700 >>> From: Jb >>> Subject: [Histonet] Controls: >>> To: Histonet@lists.utsouthwestern.edu >>> Message-ID: >>> Content-Type: text/plain; ? ?charset=us-ascii >>> >>> Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? >>> >>> Thank you, >>> >>> Sent from my iPhone >>> >>> >>> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper <@t> chla.usc.edu Thu Mar 5 12:06:05 2015 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Thu Mar 5 12:06:15 2015 Subject: [Histonet] Controls: In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D8CDE3@PAEXCH1.PathologyAssociates.local> References: <204A03EB5A7F0A4BB1EEDD52A963829C16D8CDE3@PAEXCH1.PathologyAssociates.local> Message-ID: I've used moldy orange peels as a GMS/PAS-F control in the past. Just saved the peels in a plastic bag over the weekend, then processed as usual. They worked beautifully! Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357???? Pager: 213-209-0184 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Thursday, March 05, 2015 7:21 AM To: Jb; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls: We have successfully used hamburger meat to make Gram controls. As far as GMS controls go, we ran across a post from someone smearing cream cheese onto lung tissue and letting it sit for a couple of days, fixed and processed it and were able to demonstrate Aspergillus by GMS. My fungus control stocks are low so I was actually planning to try this with some beef lung. I haven't heard of the Slim Jim method before. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Wednesday, March 04, 2015 1:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From jburch01 <@t> gmail.com Thu Mar 5 12:09:45 2015 From: jburch01 <@t> gmail.com (Jim Burchette) Date: Thu Mar 5 12:09:49 2015 Subject: [Histonet] Controls: In-Reply-To: References: <204A03EB5A7F0A4BB1EEDD52A963829C16D8CDE3@PAEXCH1.PathologyAssociates.local> Message-ID: So do onions! On Thu, Mar 5, 2015 at 12:06 PM, Cooper, Brian wrote: > I've used moldy orange peels as a GMS/PAS-F control in the past. Just > saved the peels in a plastic bag over the weekend, then processed as > usual. They worked beautifully! > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 Pager: 213-209-0184 > bcooper@chla.usc.edu > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson > Sent: Thursday, March 05, 2015 7:21 AM > To: Jb; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Controls: > > We have successfully used hamburger meat to make Gram controls. As far as > GMS controls go, we ran across a post from someone smearing cream cheese > onto lung tissue and letting it sit for a couple of days, fixed and > processed it and were able to demonstrate Aspergillus by GMS. My fungus > control stocks are low so I was actually planning to try this with some > beef lung. I haven't heard of the Slim Jim method before. > > Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb > Sent: Wednesday, March 04, 2015 1:05 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Controls: > > Off the wall question, I have been told that slim jims (pepperoni stick) > at the gas station can be processed and used as good gram controls. Has > anyone done this and do they work for GMS also? > > Thank you, > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This email and attachments may contain PHI that is privileged and > confidential and is not intended for any unauthorized person. If you, the > reader, are not the intended recipient you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. Do not read the email but instead reply to the sender and > destroy the message and any attachments. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, > please > contact the sender by reply e-mail and destroy all copies of this original > message. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jim Burchette "Fly Fishing Bum" *<'(((><* From Nancy.Stedman <@t> buschgardens.com Thu Mar 5 12:29:32 2015 From: Nancy.Stedman <@t> buschgardens.com (Stedman, Nancy) Date: Thu Mar 5 12:29:55 2015 Subject: [Histonet] Controls: In-Reply-To: References: Message-ID: <18D791D4EE07BC41BF05F8EF3CCCDE24678C8099@FTCSEAP4001.nam.int.local> Do you mean using Slim Jims right out of the package, as is, for Gram controls? What is in those things - sounds like a food safety issue! -Nancy Stedman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Thursday, March 05, 2015 1:01 PM To: 'Jb'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls: Years ago (70's and 80's) Slim Jims were used as Gram pos & neg controls in all the labs at AFIP. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Wednesday, March 04, 2015 1:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Thu Mar 5 12:33:27 2015 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Mar 5 12:33:32 2015 Subject: [Histonet] Controls: In-Reply-To: <18D791D4EE07BC41BF05F8EF3CCCDE24678C8099@FTCSEAP4001.nam.int.local> References: <18D791D4EE07BC41BF05F8EF3CCCDE24678C8099@FTCSEAP4001.nam.int.local> Message-ID: <5A2BD13465E061429D6455C8D6B40E39173567EB5A@IBMB7Exchange.digestivespecialists.com> Look at one under the scope! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stedman, Nancy Sent: Thursday, March 05, 2015 1:30 PM To: James Watson; 'Jb'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls: Do you mean using Slim Jims right out of the package, as is, for Gram controls? What is in those things - sounds like a food safety issue! -Nancy Stedman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Thursday, March 05, 2015 1:01 PM To: 'Jb'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls: Years ago (70's and 80's) Slim Jims were used as Gram pos & neg controls in all the labs at AFIP. James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Wednesday, March 04, 2015 1:05 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Controls: Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? Thank you, Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjdessoye <@t> commonwealthhealth.net Thu Mar 5 12:31:44 2015 From: mjdessoye <@t> commonwealthhealth.net (Dessoye, Michael) Date: Thu Mar 5 12:33:51 2015 Subject: [Histonet] VIP floor/counter model Message-ID: I know that most VIP's have been available in floor and countertop models. Has anyone ever looked into the possibility of converting from one type to another, or are they completely separate models? It doesn't seem like rerouting the lines to support a change like this would be a massive undertaking. Just wondering if this is possible. Thanks as always! Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Wanda.Smith <@t> HCAhealthcare.com Thu Mar 5 13:24:01 2015 From: Wanda.Smith <@t> HCAhealthcare.com (Wanda.Smith@HCAhealthcare.com) Date: Thu Mar 5 13:24:09 2015 Subject: [Histonet] RE: VIP floor/counter model In-Reply-To: References: Message-ID: <4A1B8A80249114499C2812ADCDA7250903BD88@XRDCWPMSGHCMD1A.hca.corpad.net> I was told many years ago that it is not possible. I wanted to convert a bench top to a floor model. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael Sent: Thursday, March 05, 2015 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] VIP floor/counter model I know that most VIP's have been available in floor and countertop models. Has anyone ever looked into the possibility of converting from one type to another, or are they completely separate models? It doesn't seem like rerouting the lines to support a change like this would be a massive undertaking. Just wondering if this is possible. Thanks as always! Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lacie.Algeo <@t> providence.org Thu Mar 5 13:25:44 2015 From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A) Date: Thu Mar 5 13:26:07 2015 Subject: [Histonet] Lynch syndrome Message-ID: <24C4B3C167E5694887AB594C7602CE3A03BA97BD@WN35104.or.providence.org> Hi, How many slides do you need to get started up? We have some positive colon cases for lynch syndrome that we may be able to get you some slides on. Does your pathologist prefer that you use known positive brain tissue? Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From travis <@t> imebinc.com Thu Mar 5 13:23:18 2015 From: travis <@t> imebinc.com (Travis O'Brien) Date: Thu Mar 5 13:26:29 2015 Subject: [Histonet] RE: VIP floor/counter model In-Reply-To: <4A1B8A80249114499C2812ADCDA7250903BD88@XRDCWPMSGHCMD1A.hca.corpad.net> References: <4A1B8A80249114499C2812ADCDA7250903BD88@XRDCWPMSGHCMD1A.hca.corpad.net> Message-ID: <007801d05779$d6bc7a40$84356ec0$@imebinc.com> Very possible, IMEB inc. has been doing it for years, check out their website imebinc.com! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wanda.Smith@HCAhealthcare.com Sent: Thursday, March 05, 2015 11:24 AM To: mjdessoye@commonwealthhealth.net; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: VIP floor/counter model I was told many years ago that it is not possible. I wanted to convert a bench top to a floor model. WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC? 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael Sent: Thursday, March 05, 2015 1:32 PM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] VIP floor/counter model I know that most VIP's have been available in floor and countertop models. Has anyone ever looked into the possibility of converting from one type to another, or are they completely separate models? It doesn't seem like rerouting the lines to support a change like this would be a massive undertaking. Just wondering if this is possible. Thanks as always! Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dhewitt <@t> hvhs.org Thu Mar 5 14:16:58 2015 From: dhewitt <@t> hvhs.org (Daniel Hewitt) Date: Thu Mar 5 14:17:04 2015 Subject: [Histonet] Prostate Biopsy code--One more point In-Reply-To: <0A05EC63892F834395DCC6047CAD0C5B9B62B9@UPHQMSX01.uropartners.local> References: <4034E71604330C4B8E10D1538DFB2455EC6DD7FD@BMHEXCH02.bmhvt.org><0A05EC63892F834395DCC6047CAD0C5B9B62B8@UPHQMSX01.uropartners.local> <0A05EC63892F834395DCC6047CAD0C5B9B62B9@UPHQMSX01.uropartners.local> Message-ID: <7DDB5AB36CBC574D8D680806E7BBE58B02B16B1D@MX-HVB-02.hvhs.org> We were just having a debate about these charges, if I have 20 prostate bx's can I only charge G0416 one time or 20 times? Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Thursday, March 05, 2015 11:15 AM To: Anita Buchiane; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Prostate Biopsy code--One more point Also, as of 2015, the G0416 code is used for ANY NUMBER of prostate biopsies for Medicare, not just 10-20. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff MD Sent: Thursday, March 05, 2015 10:11 AM To: Anita Buchiane; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Prostate Biopsy code For commercial, each individually submitted prostate biopsy is coded as 88305. Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anita Buchiane Sent: Thursday, March 05, 2015 10:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Prostate Biopsy code I know the CMS (Medicare) code for 10-20 prostate biopsy specimens is G0416 but what is the Cpt (non-Medicare) code? _______________________________________________________________ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. _______________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Thu Mar 5 15:01:09 2015 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Thu Mar 5 15:01:13 2015 Subject: [Histonet] Trichrome & Fixation Message-ID: Hi, It is interesting that you should mention the importance of fixation on the Trichrome stain. I have an image of two murine hearts processed, cut and stained side by side. The only difference between the two is that they were harvested at different times, so one sat in formalin long enough to be properly fixed the other one was placed in the fixative then immediately brought in to be processed from 70% ethanol on. They are *totally* different looking. The red muscle tissue looks more purple therefore less distinct from the blue blood vessels. You get a similar effect with lung bronchial epithelium. Cheers, Amos On Tue, Mar 3, 2015 at 1:00 PM, wrote: > Message: 15 > Date: Tue, 03 Mar 2015 12:34:33 -0500 > From: John Kiernan > Subject: Re: [Histonet] Masson trichrome and H and E > To: Emily Brown , > "histonet@lists.utsouthwestern.edu" > > Message-ID: <7390afa3112a.54f5aa59@uwo.ca> > Content-Type: text/plain; CHARSET=US-ASCII > > If you can't get two colours with H&E, don't expect to get the colour > scheme right with Masson's trichrome, which needs more skill. If you are > hoping to show basement membranes in the kidney, you would do better to use > a technically simpler staining method such as picro-sirius red or periodic > acid-Schiff. If for some reason you really need three colours, a one-step > trichrome such as Gomori's, Cason's or Gabe's might be the way to go rather > than Masson's or one of the other multi-step trichromes. Remember that all > trichrome methods are greatly influenced by the fixative. A post-fixation > treatment of the sections, usually with picric acid, is needed for > formaldehyde-fixed tissues. Some alternative post-fixation treatments were > proposed by Yu & Chapman (2003) J. Histotechnol. 26(2): 131-134, but their > coloured photos were not very convincing. > > Making up staining solutions in-house is always cheaper than buying > pre-made solutions. > > John Kiernan > London, Canada > From patpxs <@t> gmail.com Thu Mar 5 15:26:43 2015 From: patpxs <@t> gmail.com (Patpxs) Date: Thu Mar 5 15:26:50 2015 Subject: [Histonet] Controls In-Reply-To: References: <07.45.01004.78B78F45@mx7.cchmc.org> Message-ID: Ok, hamburger is good for what control? Paula Sent from my iPhone > On Mar 5, 2015, at 10:01 AM, Brendal Finlay wrote: > > Ok, I'm curious. Would any of these work for an AFB control? > > > Brendal C. Finlay, HT (ASCP) > Senior Histologist > Medical Center Clinic > 8333 North Davis Highway > Pensacola, FL 32514 > Phone 850.474.8581 > Fax 850.474.8584 > > -----Original Message----- > From: Garreyf > To: "Ronda Mire" > Cc: histonet@lists.utsouthwestern.edu, "Baker, Michael" > Date: 03/05/2015 11:10 > Subject: Re: [Histonet] Controls > > I am in need of both a gram and fungal control and will have to try the slim Jim, hamburger and hotdog tricks. Thanks. If they work I will post > A link to pictures. > > > Sent from my iPhone > >> On Mar 5, 2015, at 11:58 AM, Ronda Mire wrote: >> >> Slim Jim will work as a control for gram stain. Can you believe people eat this crap? >>> On Mar 5, 2015, at 11:40 AM, Baker, Michael wrote: >>> >>> Sounds like a plant spreading anti-Slim Jim propaganda. What?s next? Donut holes? >>> >>> ---- >>> Michael Baker, M.D. >>> CCHMC Pathology >>> >>>> On Mar 5, 2015, at 10:51 AM, histonet-request@lists.utsouthwestern.edu wrote: >>>> >>>> ------------------------------ >>>> >>>> Message: 9 >>>> Date: Wed, 4 Mar 2015 14:04:52 -0700 >>>> From: Jb >>>> Subject: [Histonet] Controls: >>>> To: Histonet@lists.utsouthwestern.edu >>>> Message-ID: >>>> Content-Type: text/plain; charset=us-ascii >>>> >>>> Off the wall question, I have been told that slim jims (pepperoni stick) at the gas station can be processed and used as good gram controls. Has anyone done this and do they work for GMS also? >>>> >>>> Thank you, >>>> >>>> Sent from my iPhone >>>> >>>> >>>> ------------------------------ >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ekglover <@t> comcast.net Thu Mar 5 22:29:30 2015 From: ekglover <@t> comcast.net (Kim Glover) Date: Thu Mar 5 22:30:13 2015 Subject: [Histonet] Seeking Sales & Marketing Manager Message-ID: Sales & Marketing Manager Astral Diagnostics, a subsidiary of Polysciences and a specialty chemical manufacturer of diagnostic stains and laboratory reagents serving the medical market, seeks a Sales & Marketing Manager.? Our entrepreneurial operation is adding a position to accelerate sales growth.? The ideal candidate must be able to identify and approach new private label customers with our capabilities and products.? We?re seeking a candidate who has a background in primary strategic sales development.?? Candidates need to have experience in sales of diagnostic stains and laboratory reagents that serve the medical market.? This candidate will also develop promotional literature, sales proposals and pricing; supervise customer projects for progress and budget; manage the value and relevance of our capabilities in the market place by monitoring publications and participating in trade shows and industry events.? Our ideal candidate will be business savvy with industry knowledge and a well-established network to lead the division toward new, high-value customers.? This may include industries such as histology and hematology, dental materials, diagnostic materials, veterinary products, and microbial control products. Qualifications -Bachelor?s degree in a science field from an accredited college or university -Ability and willingness to travel, mostly East Coast Region -Proven record in sales and or sales/marketing with an chemical manufacturer -Self starter with a sense of urgency -Plans, organizes, schedules, in an efficient, business like manner -Detailed oriented -Strong verbal and written communication skills We offer a competitive salary and comprehensive benefits including four medical plans, Rx, vision, dental, flexible spending accounts, 401(k) with healthy company match, life, disability, paid time off, college savings plan, long-term care insurance, and more! Must live within a commutable distance of West Deptford, NJ.? Must have legal authorization to work in the US and will not require sponsorship. EOE. M/F/D/V.? Equal opportunity employer of protected veterans and individuals with disabilities.? Drug-free workplace.? Tobacco-free work sites. Interested candidates please contact Human Resources Generalist, Monica Zortea, monica.zortea@polysciences.com From gu.lang <@t> gmx.at Fri Mar 6 02:12:32 2015 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Mar 6 02:12:37 2015 Subject: AW: [Histonet] Trichrome & Fixation In-Reply-To: References: Message-ID: <001c01d057e5$4cad7db0$e6087910$@gmx.at> Hi, years ago we did a stain called CAB (=one step trichrome) regularly on liver-tissue. I don't know if it was because of ignorance or with aim, but it was done without Bouin. The result was blue-grey hepatocytes and darker blue collagen. - also totally different to the result with Bouin (red hepatocytes). I think the Bouin is less a "re-fixation" than more an "binding-site retrival" in this context. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Amos Brooks Gesendet: Donnerstag, 05. M?rz 2015 22:01 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Trichrome & Fixation Hi, It is interesting that you should mention the importance of fixation on the Trichrome stain. I have an image of two murine hearts processed, cut and stained side by side. The only difference between the two is that they were harvested at different times, so one sat in formalin long enough to be properly fixed the other one was placed in the fixative then immediately brought in to be processed from 70% ethanol on. They are *totally* different looking. The red muscle tissue looks more purple therefore less distinct from the blue blood vessels. You get a similar effect with lung bronchial epithelium. Cheers, Amos On Tue, Mar 3, 2015 at 1:00 PM, wrote: > Message: 15 > Date: Tue, 03 Mar 2015 12:34:33 -0500 > From: John Kiernan > Subject: Re: [Histonet] Masson trichrome and H and E > To: Emily Brown , > "histonet@lists.utsouthwestern.edu" > > Message-ID: <7390afa3112a.54f5aa59@uwo.ca> > Content-Type: text/plain; CHARSET=US-ASCII > > If you can't get two colours with H&E, don't expect to get the colour > scheme right with Masson's trichrome, which needs more skill. If you > are hoping to show basement membranes in the kidney, you would do > better to use a technically simpler staining method such as > picro-sirius red or periodic acid-Schiff. If for some reason you > really need three colours, a one-step trichrome such as Gomori's, > Cason's or Gabe's might be the way to go rather than Masson's or one > of the other multi-step trichromes. Remember that all trichrome > methods are greatly influenced by the fixative. A post-fixation > treatment of the sections, usually with picric acid, is needed for > formaldehyde-fixed tissues. Some alternative post-fixation treatments > were proposed by Yu & Chapman (2003) J. Histotechnol. 26(2): 131-134, but their coloured photos were not very convincing. > > Making up staining solutions in-house is always cheaper than buying > pre-made solutions. > > John Kiernan > London, Canada > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Mar 6 08:54:54 2015 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Mar 6 08:55:00 2015 Subject: AW: [Histonet] Trichrome & Fixation In-Reply-To: References: <001c01d057e5$4cad7db0$e6087910$@gmx.at> Message-ID: <000001d0581d$823435e0$869ca1a0$@gmx.at> I disagree. Usually a dye binds covalently to the mordant, and a mordant should be a multi-valent metal (like aluminium, ferrum, molybden,..). The dye-mordant-complex binds via the mordant to the substrate. Picric acid of Bouin's is washed out before staining. It may be a matter of definition of the term mordant. Gudrun -----Urspr?ngliche Nachricht----- Von: Johnson, Carole [mailto:cjohnson@nmda.nmsu.edu] Gesendet: Freitag, 06. M?rz 2015 15:10 An: gu.lang@gmx.at Betreff: RE: [Histonet] Trichrome & Fixation Boiuns solution acts as a mordant in trichrome stains -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Friday, March 06, 2015 1:13 AM To: 'Amos Brooks' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Trichrome & Fixation Hi, years ago we did a stain called CAB (=one step trichrome) regularly on liver-tissue. I don't know if it was because of ignorance or with aim, but it was done without Bouin. The result was blue-grey hepatocytes and darker blue collagen. - also totally different to the result with Bouin (red hepatocytes). I think the Bouin is less a "re-fixation" than more an "binding-site retrival" in this context. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Amos Brooks Gesendet: Donnerstag, 05. M?rz 2015 22:01 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Trichrome & Fixation Hi, It is interesting that you should mention the importance of fixation on the Trichrome stain. I have an image of two murine hearts processed, cut and stained side by side. The only difference between the two is that they were harvested at different times, so one sat in formalin long enough to be properly fixed the other one was placed in the fixative then immediately brought in to be processed from 70% ethanol on. They are *totally* different looking. The red muscle tissue looks more purple therefore less distinct from the blue blood vessels. You get a similar effect with lung bronchial epithelium. Cheers, Amos On Tue, Mar 3, 2015 at 1:00 PM, wrote: > Message: 15 > Date: Tue, 03 Mar 2015 12:34:33 -0500 > From: John Kiernan > Subject: Re: [Histonet] Masson trichrome and H and E > To: Emily Brown , > "histonet@lists.utsouthwestern.edu" > > Message-ID: <7390afa3112a.54f5aa59@uwo.ca> > Content-Type: text/plain; CHARSET=US-ASCII > > If you can't get two colours with H&E, don't expect to get the colour > scheme right with Masson's trichrome, which needs more skill. If you > are hoping to show basement membranes in the kidney, you would do > better to use a technically simpler staining method such as > picro-sirius red or periodic acid-Schiff. If for some reason you > really need three colours, a one-step trichrome such as Gomori's, > Cason's or Gabe's might be the way to go rather than Masson's or one > of the other multi-step trichromes. Remember that all trichrome > methods are greatly influenced by the fixative. A post-fixation > treatment of the sections, usually with picric acid, is needed for > formaldehyde-fixed tissues. Some alternative post-fixation treatments > were proposed by Yu & Chapman (2003) J. Histotechnol. 26(2): 131-134, but their coloured photos were not very convincing. > > Making up staining solutions in-house is always cheaper than buying > pre-made solutions. > > John Kiernan > London, Canada > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From jvickroy <@t> SpringfieldClinic.com Fri Mar 6 10:39:31 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Fri Mar 6 10:39:36 2015 Subject: [Histonet] Richard Allan Tissue Marking Dye or Cancer Diagnostics Message-ID: <9B1A1501A800064397369BD8072E6BCAB32FC1@E2K10DB.springfieldclinic.com> In the past I have always used Cancer Diagnostics marking dyes. Can someone comment on the Richard Allan dyes? Do they work as well as the Cancer diagnostic ones? Does the dye remain well after processing so that it is easily seen in the sections? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From jkiernan <@t> uwo.ca Fri Mar 6 11:08:50 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Mar 6 11:09:25 2015 Subject: AW: [Histonet] Trichrome & Fixation In-Reply-To: <7350e4f41d15b.54f9ded6@uwo.ca> References: <001c01d057e5$4cad7db0$e6087910$@gmx.at> <000001d0581d$823435e0$869ca1a0$@gmx.at> <7330b2041ce81.54f9d2ce@uwo.ca> <7290dfdf180a2.54f9d30b@uwo.ca> <7300dbfe1e174.54f9d349@uwo.ca> <7350dbb91b2b6.54f9d400@uwo.ca> <730085a41835e.54f9d4b6@uwo.ca> <7300e06f1f9a7.54f9d4f4@uwo.ca> <73c0e7a619f89.54f9d533@uwo.ca> <7340d3c61c960.54f9d571@uwo.ca> <7380fcc31aed4.54f9d5b0@uwo.ca> <7230cc441953c.54f9d5ee@uwo.ca> <72308f681e8b3.54f9d668@uwo.ca> <7230a0991dec2.54f9d6a7@uwo.ca> <7350ce211aa8b.54f9d6e5@uwo.ca> <7240a5031b72e.54f9d724@uwo.ca> <729089a61b318.54f9d762@uwo.ca> <7300c70e1db92.54f9d855@uwo.ca> <72b0e2d61fc66.54f9d893@uwo.ca> <7290e6dc18094.54f9d8d2@uwo.ca> <7380f1b21ecc9.54f9da79@uwo.ca> <7230880a195bc.54f9daf3@uwo.ca> <7350d6ad18f84.54f9db32@uwo.ca> <72b0e2cf1fe1d.54f9db71@uwo.ca> <7240933f1a39c.54f9dbb0@uwo.ca> <73b09e151e97e.54f9dc2a@uwo.ca> <724081071e3df.54f9dca6@uwo.ca> <7300c58e1b0cb.54f9dce4@uwo.ca> <73b0a09b19bf7.54f9dd5f@uwo.ca> <735096dc1d753.54f9dd9e@uwo.ca> <7340bad91d29a.54f9dddc@uwo.ca> <734087151cc67.54f9de1b@uwo.ca> <730099771c366.54f9de59@uwo.ca> <73b0a51e19fbb.54f9de97@uwo.ca> <7350e4f41d15b.54f9ded6@uwo.ca> Message-ID: <73308f811c541.54f998d2@uwo.ca> Quite right! Treating sections of formaldehyde-fixed tissue with Bouin's fluid is not mordanting; binding site retrieval is probably a more accurate term. Picric acid induces a change in the section that improves the selective uptake of the heteropolyacid and the larger dye anions by collagen and of the smaller dye anions by cytoplasm. Picric acid alone is just as good as Bouin for this purpose. The picric acid treatment has the effect of making a section of formaldehyde-fixed tissue more like a section of tissue fixed in a picric acid-containing fixative such as Bouin or Gendre. For spectacular trichrome staining you need a fixative that contains mercuric chloride, like Zenker or SuSa. An example of a mordant in biological staining is iron alum in Heidenhain's method. The subsequently applied haematein solution forms a coloured complex with the iron bound by the sections. The differentiation in iron alum solution then removes bound haematein by forming a water-soluble complex. The word "mordant" is probably best avoided for staining with pre-formed dye-metal complexes such as carmine, aluminium-haematein (haemalum), Weigert's iron-haematein and gallocyanine-chrome alum. See R.W.Horobin (1982) "Histochemistry" Stuttgart: Gustav Fischer (ISBN 3437107003 or 0407002480), page 77. John Kiernan = = = On 06/03/15, Gudrun Lang wrote: > I disagree. Usually a dye binds covalently to the mordant, and a mordant should be a multi-valent metal (like aluminium, ferrum, molybden,..). The dye-mordant-complex binds via the mordant to the substrate. > Picric acid of Bouin's is washed out before staining. > > It may be a matter of definition of the term mordant. > > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: Johnson, Carole [mailto:cjohnson@nmda.nmsu.edu] > Gesendet: Freitag, 06. M?rz 2015 15:10 > An: gu.lang@gmx.at > Betreff: RE: [Histonet] Trichrome & Fixation > > Boiuns solution acts as a mordant in trichrome stains > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang > Sent: Friday, March 06, 2015 1:13 AM > To: 'Amos Brooks' > Cc: histonet@lists.utsouthwestern.edu > Subject: AW: [Histonet] Trichrome & Fixation > > Hi, > years ago we did a stain called CAB (=one step trichrome) regularly on liver-tissue. I don't know if it was because of ignorance or with aim, but it was done without Bouin. The result was blue-grey hepatocytes and darker blue collagen. - also totally different to the result with Bouin (red hepatocytes). > > I think the Bouin is less a "re-fixation" than more an "binding-site retrival" in this context. > > Gudrun > > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Amos Brooks > Gesendet: Donnerstag, 05. M?rz 2015 22:01 > An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Trichrome & Fixation > > Hi, > It is interesting that you should mention the importance of fixation on the Trichrome stain. I have an image of two murine hearts processed, cut and stained side by side. The only difference between the two is that they were harvested at different times, so one sat in formalin long enough to be properly fixed the other one was placed in the fixative then immediately brought in to be processed from 70% ethanol on. They are *totally* different looking. The red muscle tissue looks more purple therefore less distinct from the blue blood vessels. You get a similar effect with lung bronchial epithelium. > > Cheers, > Amos > > On Tue, Mar 3, 2015 at 1:00 PM, > wrote: > > > Message: 15 > > Date: Tue, 03 Mar 2015 12:34:33 -0500 > > From: John Kiernan > > Subject: Re: [Histonet] Masson trichrome and H and E > > To: Emily Brown , > > "histonet@lists.utsouthwestern.edu" > > > > Message-ID: <7390afa3112a.54f5aa59@uwo.ca> > > Content-Type: text/plain; CHARSET=US-ASCII > > > > If you can't get two colours with H&E, don't expect to get the colour > > scheme right with Masson's trichrome, which needs more skill. If you > > are hoping to show basement membranes in the kidney, you would do > > better to use a technically simpler staining method such as > > picro-sirius red or periodic acid-Schiff. If for some reason you > > really need three colours, a one-step trichrome such as Gomori's, > > Cason's or Gabe's might be the way to go rather than Masson's or one > > of the other multi-step trichromes. Remember that all trichrome > > methods are greatly influenced by the fixative. A post-fixation > > treatment of the sections, usually with picric acid, is needed for > > formaldehyde-fixed tissues. Some alternative post-fixation treatments > > were proposed by Yu & Chapman (2003) J. Histotechnol. 26(2): 131-134, but their coloured photos were not very convincing. > > > > Making up staining solutions in-house is always cheaper than buying > > pre-made solutions. > > > > John Kiernan > > London, Canada > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From JRobinson <@t> pathology-associates.com Fri Mar 6 11:16:04 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Fri Mar 6 11:16:17 2015 Subject: [Histonet] Mushrooms for GMS fungus control Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8D09D@PAEXCH1.PathologyAssociates.local> How about mushrooms? Has anyone had any success using mushrooms as a GMS fungus control? Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From Deann.Chandler <@t> NewberryHospital.net Fri Mar 6 11:29:10 2015 From: Deann.Chandler <@t> NewberryHospital.net (Deann D. Chandler) Date: Fri Mar 6 11:29:18 2015 Subject: [Histonet] Re: CONTROLS Message-ID: <04372267D55F674D847F8D59888BB6462438014A@exchbe01.NewberryHospital.net> I once tried using Slim Jims as a control for gram staining. It had a lot of positive bacteria, but no negatives. We then went to our microbiology lab got a little colony of positive and negative bacteria, embedded it in agar and processed it. This worked great! I do want to see if this works with hamburger or hot dogs. DeAnn Chandler, BS, HT (ASCP) Histology Coordinator Newberry County Memorial Hospital From jpiche <@t> wtbyhosp.org Fri Mar 6 11:30:54 2015 From: jpiche <@t> wtbyhosp.org (Piche, Jessica) Date: Fri Mar 6 11:30:59 2015 Subject: [Histonet] RE: Mushrooms for GMS fungus control In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D8D09D@PAEXCH1.PathologyAssociates.local> References: <204A03EB5A7F0A4BB1EEDD52A963829C16D8D09D@PAEXCH1.PathologyAssociates.local> Message-ID: <631955447A364B45B9458D2905635110D7C22E17@WIN08-MBX-01.wtbyhosp.org> I will say I hacked a mushroom off of a tree once and processed it and it stained nicely for GMS. I also used some chicken that was in the fridge too long and we actually use that for our GMS control!! Jessica Piche, HT(ASCP) Waterbury Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Friday, March 06, 2015 12:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mushrooms for GMS fungus control How about mushrooms? Has anyone had any success using mushrooms as a GMS fungus control? Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From mpence <@t> grhs.net Fri Mar 6 11:37:58 2015 From: mpence <@t> grhs.net (Mike Pence) Date: Fri Mar 6 11:38:16 2015 Subject: [Histonet] RE: Mushrooms for GMS fungus control In-Reply-To: <631955447A364B45B9458D2905635110D7C22E17@WIN08-MBX-01.wtbyhosp.org> References: <204A03EB5A7F0A4BB1EEDD52A963829C16D8D09D@PAEXCH1.PathologyAssociates.local> <631955447A364B45B9458D2905635110D7C22E17@WIN08-MBX-01.wtbyhosp.org> Message-ID: As your fungal control? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Friday, March 06, 2015 11:32 AM To: Jeffrey Robinson; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Mushrooms for GMS fungus control I will say I hacked a mushroom off of a tree once and processed it and it stained nicely for GMS. I also used some chicken that was in the fridge too long and we actually use that for our GMS control!! Jessica Piche, HT(ASCP) Waterbury Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Friday, March 06, 2015 12:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mushrooms for GMS fungus control How about mushrooms? Has anyone had any success using mushrooms as a GMS fungus control? Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sally.norton <@t> seattlechildrens.org Fri Mar 6 12:50:09 2015 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Fri Mar 6 12:50:30 2015 Subject: [Histonet] RE: Mushrooms for GMS fungus control In-Reply-To: References: <204A03EB5A7F0A4BB1EEDD52A963829C16D8D09D@PAEXCH1.PathologyAssociates.local> <631955447A364B45B9458D2905635110D7C22E17@WIN08-MBX-01.wtbyhosp.org> Message-ID: <1357F84B33D39A46BA015A8EC6ABCBD040EB5817@PPWEXD01a.childrens.sea.kids> We have used orange rind and brie - left to grow mold - as controls in a pinch. Our docs preferred the cheese. Sally Norton Seattle Children's Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Friday, March 06, 2015 9:38 AM To: 'Piche, Jessica'; Jeffrey Robinson; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Mushrooms for GMS fungus control As your fungal control? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Friday, March 06, 2015 11:32 AM To: Jeffrey Robinson; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Mushrooms for GMS fungus control I will say I hacked a mushroom off of a tree once and processed it and it stained nicely for GMS. I also used some chicken that was in the fridge too long and we actually use that for our GMS control!! Jessica Piche, HT(ASCP) Waterbury Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Friday, March 06, 2015 12:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mushrooms for GMS fungus control How about mushrooms? Has anyone had any success using mushrooms as a GMS fungus control? Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From blayjorge <@t> gmail.com Fri Mar 6 14:49:10 2015 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Fri Mar 6 14:49:14 2015 Subject: [Histonet] Stain vs dye and control Message-ID: Dear Histonetters: Last semester I taught a microbiology lab and, as I was reviewing for class, noticed some lack of precision in the use of the terms "dye" vs. "stain" in biology. Could someone help? While I am on "stains", I have been following the emails on controls and wonder a couple of things. 1. What would testing for bacteria on beef jerky, hot dogs or burgers accomplish that is different (ideally better) that what one accomplishes by pulling out from fresh cultures out of a medium (e.g. liquid, such as broth, solid, such as slabs, agar)? Is it the idea to test for bacteria in an animal tissue? If so, would a solid medium (like someone mentioned recently, such as agar) do? 2. An advantage of using fresh bacterial cultures of known Gram is that it could be used to test whether the reagents are good enough. Last semester I had the suspicion that one (or more) of our Gram reagents where not up to par. If you have any feedback, please feel free to email me directly at blayjorge@gmail.com . Thank you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From rjbuesa <@t> yahoo.com Fri Mar 6 15:11:00 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 6 15:11:05 2015 Subject: [Histonet] Stain vs dye and control In-Reply-To: References: Message-ID: <1251964164.4230132.1425676260423.JavaMail.yahoo@mail.yahoo.com> In a very simplistic way, a "dye" is the active chemical substance (usually an aniline powder) that you use to prepare a staining solution for certain "stain" procedure or method."Dye" is a more "precise" term but stain had "many shades of color"! Ren? J.? On Friday, March 6, 2015 3:49 PM, Jorge A. Santiago-Blay wrote: Dear Histonetters: Last semester I taught a microbiology lab and, as I was reviewing for class, noticed some lack of precision in the use of the terms "dye" vs. "stain" in biology. Could someone help? While I am on "stains", I have been following the emails on controls and wonder a couple of things. 1. What would testing for bacteria on beef jerky, hot dogs or burgers accomplish that is different (ideally better) that what one accomplishes by pulling out from fresh cultures out of a medium (e.g. liquid, such as broth, solid, such as slabs, agar)?? Is it the idea to test for bacteria in an animal tissue? If so, would a solid medium (like someone mentioned recently, such as agar) do? 2. An advantage of using fresh bacterial cultures of known Gram is that it could be used to test whether the reagents are good enough. Last semester I had the suspicion that one (or more) of our Gram reagents where not up to par. If you have any feedback, please feel free to email me directly at blayjorge@gmail.com . Thank you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Sun Mar 8 01:38:56 2015 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Mar 8 01:39:03 2015 Subject: [Histonet] Considerations about Warthin-Starry principle Message-ID: <000001d05972$ef74d6e0$ce5e84a0$@gmx.at> Dear histonetters, I came across a question regarding the Warthin-Starry principle. Are bacterial catalase or oxidase the cause for developement of silver-deposits? The usual explanation is, that bacteria are able to bind silver onto their surface, that is further reduced by hydrochinon to visible silver-deposits. But why? Some considerations: The pH must be held at pH4 with citric acid in the WS developement-solution. (Is this the pH-optimum for the bacterial enzymes?) IHC shows that endogenous peroxidase survives the embedding-process. (Is this also true for the bacterial enzymes?) Spirochetes (and others) are catalase and oxidase positive. Silver-In situ hybdridisation technique shows, that peroxidase with H2O2 can be used to reduce Silveracetate for silver-deposits. Is there a possible relationship between the bacterial enzymes and the WS principle? Or am I totally on an erroneous way? What do you think? thanks for any input. Gudrun Lang From gu.lang <@t> gmx.at Sun Mar 8 03:02:01 2015 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Mar 8 03:02:08 2015 Subject: AW: [Histonet] Stain vs dye and control In-Reply-To: References: Message-ID: <000501d05976$295195d0$7bf4c170$@gmx.at> Hi Jorge, Not all histolabs have access to fresh cultures. So they search an easy way to get bacterial controls. And things like sausage are more like the usual specimens than liquid samples. The best staining control is an inhouse-specimen, that is processed in the same way as any other specimen (preanalytic, fixation, processing, cutting, staining). But sometimes this is not possible, so one uses the "next-best". A control with known ingredients (like bacteria) can be used to check the whole process and must be positive for the tested parameter. A patient-sample can only be considered positive or negative, if the positive-control proves the functionality of the process (staining-protocol). Dye and stain. You can touch the dye, but not the stain. And then you have got stained fingers. ;-) Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jorge A. Santiago-Blay Gesendet: Freitag, 06. M?rz 2015 21:49 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] Stain vs dye and control Dear Histonetters: Last semester I taught a microbiology lab and, as I was reviewing for class, noticed some lack of precision in the use of the terms "dye" vs. "stain" in biology. Could someone help? While I am on "stains", I have been following the emails on controls and wonder a couple of things. 1. What would testing for bacteria on beef jerky, hot dogs or burgers accomplish that is different (ideally better) that what one accomplishes by pulling out from fresh cultures out of a medium (e.g. liquid, such as broth, solid, such as slabs, agar)? Is it the idea to test for bacteria in an animal tissue? If so, would a solid medium (like someone mentioned recently, such as agar) do? 2. An advantage of using fresh bacterial cultures of known Gram is that it could be used to test whether the reagents are good enough. Last semester I had the suspicion that one (or more) of our Gram reagents where not up to par. If you have any feedback, please feel free to email me directly at blayjorge@gmail.com . Thank you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barryrittman <@t> gmail.com Sun Mar 8 09:18:04 2015 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Sun Mar 8 09:18:08 2015 Subject: [Histonet] Stain vs dye and control In-Reply-To: <000501d05976$295195d0$7bf4c170$@gmx.at> References: <000501d05976$295195d0$7bf4c170$@gmx.at> Message-ID: There is always confusion about this. In textile "coloring" the term dyeing is used and this first carried over to histology as many of the textile dyes were the ones first used to stain tissue components. Some of these were specific and some non specific. In histology my understanding is that the dye is the powder, while the stain is the solution used for staining the tissues. and in most cases there is a certain amount of specificity in the reaction. Merely imparting a non specific color all components of a tissue I would regard as coloring as there is no specificity in this process. This raised the interesting question of what would you call the process whereby osmium tetroxide is used to fix and stain some fats? To further complicate in the concrete industry the term dye is used to indicate a general coloring of the concrete while the term stain is used to indicate a chemical reaction with the concrete components. Barry On Sun, Mar 8, 2015 at 3:02 AM, Gudrun Lang wrote: > Hi Jorge, > Not all histolabs have access to fresh cultures. So they search an easy > way to get bacterial controls. And things like sausage are more like the > usual specimens than liquid samples. > > The best staining control is an inhouse-specimen, that is processed in the > same way as any other specimen (preanalytic, fixation, processing, cutting, > staining). But sometimes this is not possible, so one uses the "next-best". > A control with known ingredients (like bacteria) can be used to check the > whole process and must be positive for the tested parameter. A > patient-sample can only be considered positive or negative, if the > positive-control proves the functionality of the process > (staining-protocol). > > Dye and stain. You can touch the dye, but not the stain. And then you > have got stained fingers. ;-) > > Gudrun > > > > -----Urspr?ngliche Nachricht----- > Von: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jorge A. > Santiago-Blay > Gesendet: Freitag, 06. M?rz 2015 21:49 > An: Histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Stain vs dye and control > > Dear Histonetters: > > Last semester I taught a microbiology lab and, as I was reviewing for > class, noticed some lack of precision in the use of the terms "dye" vs. > "stain" in biology. Could someone help? > > While I am on "stains", I have been following the emails on controls and > wonder a couple of things. > > 1. What would testing for bacteria on beef jerky, hot dogs or burgers > accomplish that is different (ideally better) that what one accomplishes by > pulling out from fresh cultures out of a medium (e.g. liquid, such as > broth, solid, such as slabs, agar)? Is it the idea to test for bacteria in > an animal tissue? If so, would a solid medium (like someone mentioned > recently, such as agar) do? > > 2. An advantage of using fresh bacterial cultures of known Gram is that it > could be used to test whether the reagents are good enough. Last semester I > had the suspicion that one (or more) of our Gram reagents where not up to > par. > > If you have any feedback, please feel free to email me directly at > blayjorge@gmail.com . Thank you. > > Sincerely, > > Jorge > > Jorge A. Santiago-Blay, PhD > blaypublishers.com > http://blayjorge.wordpress.com/ > http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lindamargraf <@t> gmail.com Sun Mar 8 14:56:07 2015 From: lindamargraf <@t> gmail.com (Linda Margraf) Date: Sun Mar 8 14:56:11 2015 Subject: [Histonet] FW: Masson's trichrome stain In-Reply-To: References: Message-ID: <016501d059d9$eb25c240$c17146c0$@gmail.com> Here is a message from Justine... From: Justine Lanzon [mailto:justinelanzon@hotmail.com] Sent: Thursday, March 05, 2015 5:36 AM To: lindamargraf@gmail.com Subject: Masson's trichrome stain Hi, I am doing a write up on Masson's trichrome stain however I cannot answer these two questions: - Why are plastic forceps used instead of metal ones to hold the stained slide? - Why do we not rinse before Alinine blue? Can you please help me? Many Thanks, Justine Lanzon From linda.prasad <@t> health.nsw.gov.au Sun Mar 8 18:09:02 2015 From: linda.prasad <@t> health.nsw.gov.au (Linda Prasad (SCHN)) Date: Sun Mar 8 18:09:20 2015 Subject: [Histonet] RE: Mushrooms for GMS fungus control In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D8D09D@PAEXCH1.PathologyAssociates.local> References: <204A03EB5A7F0A4BB1EEDD52A963829C16D8D09D@PAEXCH1.PathologyAssociates.local> Message-ID: <1217DDB3D7DE5E418E3D560A268EABD0A27E33F3@xmdb03.nch.kids> I used strawberries for a fungal control. Worked really good. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???Please consider the environment before printing this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Saturday, 7 March 2015 4:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mushrooms for GMS fungus control How about mushrooms? Has anyone had any success using mushrooms as a GMS fungus control? Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From koellingr <@t> comcast.net Sun Mar 8 18:28:39 2015 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Sun Mar 8 18:28:54 2015 Subject: [Histonet] RE: Mushrooms for GMS fungus control In-Reply-To: <1217DDB3D7DE5E418E3D560A268EABD0A27E33F3@xmdb03.nch.kids> References: <204A03EB5A7F0A4BB1EEDD52A963829C16D8D09D@PAEXCH1.PathologyAssociates.local> <1217DDB3D7DE5E418E3D560A268EABD0A27E33F3@xmdb03.nch.kids> Message-ID: <361538743.1794064.1425857319460.JavaMail.zimbra@comcast.net> Apparently there are numerous interesting ways for fungus or bacteria controls to be had from orange peels to hamburger to slim Jim's to hot dogs to strawberries to ????.? Sounds like fun to me.? I'm curious, with the emphasis now on quality control in labs?run amok, has anyone passed a rigorous inspection actually showing these as your currently in-use controls?? A PI in research who?doesn't want?his paper rejected at peer review.? A CAP inspector in clinical labs who is nit-picky reviewing staining controls but might be looking for a phase anything deficiency.? The?dot-your-i's and cross-your-t's?FDA people who might or might not OK your drug in development.? Really, just curious if anyone with a hammer over your head has said it is perfectly fine to use them. Ray, Seattle, WA ----- Original Message ----- From: "Linda Prasad (SCHN)" To: "Jeffrey Robinson" , histonet@lists.utsouthwestern.edu Sent: Sunday, March 8, 2015 4:09:02 PM Subject: [Histonet] RE: Mushrooms for GMS fungus control I used strawberries for a fungal control. Worked really good. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???Please consider the environment before printing this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Saturday, 7 March 2015 4:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mushrooms for GMS fungus control How about mushrooms? ?Has anyone had any success using mushrooms as a GMS fungus control? Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Sun Mar 8 19:27:12 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Sun Mar 8 19:27:17 2015 Subject: [Histonet] CSH Symposium Message-ID: The California Society for Histotechnology will be hosting their 39th Annual Symposium at the Hilton Scotts Valley/Santa Cruz May 1-3. The link below will take you to our website. CE certificates will be issued at the completion of each workshop for those that have pre-registered for the event. The program can also be emailed to you if you contact me. Jennifer http://www.californiahistology.org/events.html From relia1 <@t> earthlink.net Mon Mar 9 09:38:39 2015 From: relia1 <@t> earthlink.net (Pam Barker) Date: Mon Mar 9 09:38:46 2015 Subject: [Histonet] Tomorrow Tuesday March 10 is Histotechnology Professionals Day! Message-ID: <005301d05a76$bbf17a30$33d46e90$@earthlink.net> Hi Histonetters!!! Did you know that tomorrow Tuesday, March 10 is Histotechnology Professionals Day? The National Society for Histotechnology has some great suggestions for ways to celebrate. Here is the link: http://www.nsh.org/content/histotechnology-professionals-day What are you doing to celebrate? I started last week by contacting each and every one of my 1000+ client/employers to let them know that March 10 is the 6th Annual Histotechnology Professionals Day. Hopefully, if they weren't already doing something to show their appreciation they are now!! In honor of Histotechnology Professionals Day. RELIA Solutions celebrates 3 years as Sustaining member of the National Society of Histotechnology. And on Friday I will be lecturing to a group of soon to be histology school graduates on interviewing and job hunting! As always I have amazing opportunities with outstanding clients that I want to tell you about- here is a quick list of the positions I am most excited about. All of these are full time permanent opportunities and our clients offer excellent compensation, benefits and in most cases relo/sign on bonuses. Here is the list of my HOTTEST OPPORTUNITIES !! Histology Leadership Opportunities: Histology Supervisor - Flagstaff, AZ AP Manager - Chicago, IL Histo Supervisor Long Island, NY; (NY license NOT required) Supervisors also needed in MA, MO, and IL Histology Technician/Technologist Histotechnician - Hammond, IN (ASCP OR ELIGIBLE) ***Dermpath Histotech Milwaukee BRAND NEW LAB RELIA EXCLUSIVE!!*** Dermpath Histotech - Kansas, City, KS (ENTRY LEVEL OR EXPERIENCED) Histotechs also needed in TN, MA, NY, OH and VA Maybe it is a good time to reflect on your career, where you are, what you want, where you would like to be and how are you going to get there? If you aren't considering a job change: Is it time to join/renew your NSH membership? Check out your state society? Join the Histonet? Take some CEUs to stay up to date and learn some new skills? Get that state license for the place you want to move to one day? Start studying for that QIHC? If you are or might be considering a job change now or in the near future: Does your resume need to be updated? Have you considered what you are looking for and where? Have you gotten the state license if it is required before you go? Have we spoken recently? Please feel free to contact me by phone toll free at 866-607-3542 or email: relia1@earthlink.net if you want information or help with any of the items listed. My services are always free of charge to you. If you have any friends that might benefit from receiving this please feel free to pass it along. Have A Happy Histo Day!! >Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From gu.lang <@t> gmx.at Mon Mar 9 10:01:55 2015 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Mar 9 10:02:06 2015 Subject: AW: [Histonet] FW: Masson's trichrome stain In-Reply-To: <016501d059d9$eb25c240$c17146c0$@gmail.com> References: <016501d059d9$eb25c240$c17146c0$@gmail.com> Message-ID: <000001d05a79$fc05b700$f4112500$@gmx.at> Maybe I am wrong, but I think the usage of plastic forceps for trichrome stains is nonsense. I only know this from silver stains, but also have never seen problems personally. The polyacids have to stay onto the section, when anilinblue is added. There exists a theory, that PMA/PTA acts like a mordant and builds bridges between collagen and anilinblue. It is proven, that PMA is not washed out of the tissue and is bound to the fibers. On the other hand it seems, that the polyacids prevent the staining of globular proteins by anilinblue (the longer the polyacids the brighter the red colour). It also helps to keep the low staining-pH of anilinblue. Gudrun Lang From: Justine Lanzon [mailto:justinelanzon@hotmail.com] Sent: Thursday, March 05, 2015 5:36 AM To: lindamargraf@gmail.com Subject: Masson's trichrome stain Hi, I am doing a write up on Masson's trichrome stain however I cannot answer these two questions: - Why are plastic forceps used instead of metal ones to hold the stained slide? - Why do we not rinse before Alinine blue? Can you please help me? Many Thanks, Justine Lanzon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick <@t> northwestern.edu Mon Mar 9 14:41:48 2015 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Mon Mar 9 14:41:54 2015 Subject: [Histonet] Old slides. Message-ID: Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu From jmcgough <@t> clinlab.com Mon Mar 9 15:20:09 2015 From: jmcgough <@t> clinlab.com (=?utf-8?Q?Jason_McGough?=) Date: Mon Mar 9 15:12:20 2015 Subject: [Histonet] Old slides. In-Reply-To: References: Message-ID: Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgough@clinlab.com www.clinlab.com -----Original message----- > From:Bernice Frederick > > Sent: Monday, March 9, 2015 1:51 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Old slides. > > Hi all, > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. > Thanks, > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Timothy.Morken <@t> ucsf.edu Mon Mar 9 17:46:08 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Mon Mar 9 17:46:15 2015 Subject: [Histonet] RE: Mushrooms for GMS fungus control In-Reply-To: <361538743.1794064.1425857319460.JavaMail.zimbra@comcast.net> References: <204A03EB5A7F0A4BB1EEDD52A963829C16D8D09D@PAEXCH1.PathologyAssociates.local> <1217DDB3D7DE5E418E3D560A268EABD0A27E33F3@xmdb03.nch.kids> <361538743.1794064.1425857319460.JavaMail.zimbra@comcast.net> Message-ID: <761E2B5697F795489C8710BCC72141FF367FBA31@ex07.net.ucsf.edu> Try this article... Acta Cytol. 2003 Nov-Dec;47(6):1043-4. Alternative, cost-effective fungus-staining method for control slides in cytology and histopathology. da Silva VD1. Author information Abstract OBJECTIVE: To develop a cost-effective, reliable and safe method of providing fungal control slides for routine use in pathology laboratories. STUDY DESIGN: A set of easily available, low-cost material was tested to obtain fungal colonies on substrate adequate for paraffin-embedded sections or smears. RESULTS: Such material as cheese is a simple, inexpensive and practical culture medium for silver-positive fungi. A batch of paraffin blocks can be prepared to maintain a stock of control material in the laboratory. CONCLUSION: It is useful to maintain fungal colonies to produce staining control specimens using small pieces of refrigerated cheese to easily produce silver-staining control specimens or smears embedded in paraffin, reducing the risk of accidental exposure to potentially infective pathogens in the laboratory. This method might also be a good alternative for conserving routine surgical specimens, considering the currently decreasing numbers of necropsy and large specimens, particularly from immunosuppressed and infected patients. PMID: 14674076 [PubMed - indexed for MEDLINE] -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Sunday, March 08, 2015 4:29 PM To: Linda Prasad (SCHN) Cc: Jeffrey Robinson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Mushrooms for GMS fungus control Apparently there are numerous interesting ways for fungus or bacteria controls to be had from orange peels to hamburger to slim Jim's to hot dogs to strawberries to ????.? Sounds like fun to me.? I'm curious, with the emphasis now on quality control in labs?run amok, has anyone passed a rigorous inspection actually showing these as your currently in-use controls?? A PI in research who?doesn't want?his paper rejected at peer review.? A CAP inspector in clinical labs who is nit-picky reviewing staining controls but might be looking for a phase anything deficiency.? The?dot-your-i's and cross-your-t's?FDA people who might or might not OK your drug in development.? Really, just curious if anyone with a hammer over your head has said it is perfectly fine to use them. Ray, Seattle, WA ----- Original Message ----- From: "Linda Prasad (SCHN)" To: "Jeffrey Robinson" , histonet@lists.utsouthwestern.edu Sent: Sunday, March 8, 2015 4:09:02 PM Subject: [Histonet] RE: Mushrooms for GMS fungus control I used strawberries for a fungal control. Worked really good. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???Please consider the environment before printing this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Saturday, 7 March 2015 4:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mushrooms for GMS fungus control How about mushrooms? ?Has anyone had any success using mushrooms as a GMS fungus control? Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Tue Mar 10 00:31:56 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Mar 10 00:32:00 2015 Subject: [Histonet] FW: Masson's trichrome stain In-Reply-To: <7040da314c523.54fe81bb@uwo.ca> References: <016501d059d9$eb25c240$c17146c0$@gmail.com> <7040a27a4853e.54fe7d5d@uwo.ca> <7030c71d49373.54fe7dd6@uwo.ca> <72d09bca4f592.54fe7e14@uwo.ca> <7380c92c4b87a.54fe7e53@uwo.ca> <7020a7064f48f.54fe7e93@uwo.ca> <73b0ef064c8a3.54fe7ed1@uwo.ca> <738097214a7e8.54fe7f0f@uwo.ca> <7380cfbd49ac1.54fe7f4d@uwo.ca> <7040f2664acc0.54fe7f8c@uwo.ca> <7040911f4a4b5.54fe7fca@uwo.ca> <7040a5c84ed0c.54fe8008@uwo.ca> <73c0bd4949b82.54fe8046@uwo.ca> <7380df684a38f.54fe8084@uwo.ca> <7030b1b44eb49.54fe80c2@uwo.ca> <7030c95c48e9c.54fe8101@uwo.ca> <73b090ae4c3c7.54fe813f@uwo.ca> <72e0b3ec4a7ac.54fe817d@uwo.ca> <7040da314c523.54fe81bb@uwo.ca> Message-ID: <7380eaed48941.54fe3b7c@uwo.ca> The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? John Kiernan = = = On 08/03/15, Linda Margraf wrote: > Here is a message from Justine... > > From: Justine Lanzon [mailto:justinelanzon@hotmail.com] > Sent: Thursday, March 05, 2015 5:36 AM > To: lindamargraf@gmail.com > Subject: Masson's trichrome stain > > > Hi, > > I am doing a write up on Masson's trichrome stain however I cannot answer > these two questions: > > - Why are plastic forceps used instead of metal ones to hold the stained > slide? > > - Why do we not rinse before Alinine blue? > > ? > > Can you please help me? > > ? > > Many Thanks, > > Justine Lanzon > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jkiernan <@t> uwo.ca Tue Mar 10 00:40:02 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Tue Mar 10 00:40:06 2015 Subject: [Histonet] Old slides. In-Reply-To: <7020b5654eae5.54fe8395@uwo.ca> References: <73e0c5634f4c8.54fe8260@uwo.ca> <70309a1c4dd84.54fe829d@uwo.ca> <7300bf984f428.54fe82db@uwo.ca> <73c0802648262.54fe8319@uwo.ca> <73c0e7ec4ba47.54fe8357@uwo.ca> <7020b5654eae5.54fe8395@uwo.ca> Message-ID: <73e09aa74b442.54fe3d62@uwo.ca> Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one. John Kiernan Anatomy & Cell Biology, UWO London, Canada = = = On 09/03/15, Jason McGough wrote: > Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. > > > > Jason McGough, HT(ASCP) > > Operations Manager > > Clinical Laboratory of the Black Hills > > 605-343-2267 > > jmcgough@clinlab.com > > > www.clinlab.com > > ? > ? > -----Original message----- > > From:Bernice Frederick > > > > Sent: Monday, March 9, 2015 1:51 PM > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] Old slides. > > > > Hi all, > > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From richard.wild <@t> wanadoo.fr Tue Mar 10 04:05:11 2015 From: richard.wild <@t> wanadoo.fr (richard wild) Date: Tue Mar 10 04:05:38 2015 Subject: [Histonet] soft for microwriter (thermo scientific, Lamb, Shandon) Message-ID: <54FEB3C7.40306@wanadoo.fr> Hi I am looking for labelling software (or advices) for the carousel microwriter (thermo scientific, Lamb, Shandon = same machine) (LAMB E22.01MWR) The machine is discontinuated. I would like to use serial interface rs232 and barcode scanners. Thanks for help. Richard From b.curran.mcwilliam <@t> gmail.com Tue Mar 10 06:51:25 2015 From: b.curran.mcwilliam <@t> gmail.com (b.curran.mcwilliam@gmail.com) Date: Tue Mar 10 06:51:28 2015 Subject: [Histonet] Old slides. In-Reply-To: References: Message-ID: <9FA4B5FB-11BE-47C7-AA0C-6F8416B10487@gmail.com> Hi We re-Coverslipper a number of sections which had peeled off on the tape as the tape dried and curled. We cut off excess tape using scissors; placed a fresh coverslip flat; put a streak of mounting medium on he coverslip; use a forceps to orientate the tissue + margin of tape; number a slide, dip it in Xylene & place on a slope & bring on top of coverslip-section-mounting medium; turn the slide-section- coverslip to face coverslip up; leave horizontal to dry (eg overnight). Worth a try, doing one first. Bernie, St Vincent's, Dublin, Ireland > On 9 Mar 2015, at 19:41, Bernice Frederick wrote: > > Hi all, > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. > Thanks, > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 10 08:43:35 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 10 08:43:40 2015 Subject: [Histonet] Old slides. In-Reply-To: <73e09aa74b442.54fe3d62@uwo.ca> References: <73e09aa74b442.54fe3d62@uwo.ca> Message-ID: <1897656965.1753669.1425995015431.JavaMail.yahoo@mail.yahoo.com> To John Probably the writer is referring to slides "mounted" with the Sakura film?"coverslip".I have done it many times and the FILM will?detach easily from the section.Had it been a glass coverslip attached with, for example, Permount, acetone would not have done anything.Ren? J.? On Tuesday, March 10, 2015 1:40 AM, John Kiernan wrote: Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one. John Kiernan Anatomy & Cell Biology, UWO London, Canada = = = On 09/03/15, Jason McGough? wrote: > Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. > > > > Jason McGough, HT(ASCP) > > Operations Manager > > Clinical Laboratory of the Black Hills > > 605-343-2267 > > jmcgough@clinlab.com > > > www.clinlab.com > > ? > ? > -----Original message----- > > From:Bernice Frederick > > > > Sent: Monday, March 9, 2015 1:51 PM > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] Old slides. > > > > Hi all, > > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christiecgowan <@t> dermatology.med.ufl.edu Mon Mar 9 15:01:10 2015 From: christiecgowan <@t> dermatology.med.ufl.edu (Gowan,Christie C) Date: Tue Mar 10 11:12:07 2015 Subject: [Histonet] RE: Old slides. In-Reply-To: References: Message-ID: Hi Bernice, I have found that if you flood the slide with mounting media (don't use xylene) flip the slide over onto an absorbent lab wipe and put a heavy weight with even pressure and leave for a few hours. If the slide sticks to the wipe just put a few drops of xylene to clean up the slide. You may still have some tiny bubbles but it is much better than the alternative. Good luck. Christie Gowan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, March 09, 2015 3:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From esarricks <@t> gmail.com Tue Mar 10 11:12:05 2015 From: esarricks <@t> gmail.com (Erin Sarricks) Date: Tue Mar 10 11:12:13 2015 Subject: [Histonet] IHC / Morphometry Technician wanted in Shenandoah Valley Virginia Message-ID: Histology Laboratory located in the Shenandoah Valley of Virginia is looking to add to it's team. In this position, you will need a working knowledge of IHC theory and practical IHC experience. The best candidate for the position will oversee immunohistochemical staining as well as perform other histology functions including trimming of specimens, paraffin embedding, microtomy and microscopic QC of slides. Experience with morphometry is preferable. Desirable candidates will possess the following: - HT (ASCP) or QIHC registration preferred - 4 years of Histology experience - 1+ years of immunohistochemistry and/or immunofluorescence experience - Keeps abreast with company's current policies and immunohistochemistry technical updates and procedures - Must be able to work independently and in a team environment Full time employment benefits include subsidized medical and dental insurance, vacation, holiday pay, and 401k after 1 year of employment. Compensation is commensurate with experience. If you are interested in this position, please respond to this post with your resume and cover letter. From sforeman <@t> labpath.com Tue Mar 10 12:15:05 2015 From: sforeman <@t> labpath.com (Susan Foreman) Date: Tue Mar 10 12:18:16 2015 Subject: [Histonet] anti-BRAF V600E (VE1) Ventana Message-ID: <00bd01d05b55$c09b85f0$41d291d0$@labpath.com> What vendor are you guys using for antibody anti-BRAF V600E (VE1)? Spring Bioscience or Ventana? What dilution are you using? Are you utilizing amplification? Expensive From suetp918 <@t> comcast.net Tue Mar 10 12:31:58 2015 From: suetp918 <@t> comcast.net (suetp918) Date: Tue Mar 10 12:32:44 2015 Subject: [Histonet] RE: Old slides. Message-ID: So we actually cut the film around the section and mount to another slide and do pretty much what wascabove mentioned placing upside downband placing on paper towel. ?Actually works pretty good. TJUH Sue Paturzo Sent from my Verizon Wireless 4G LTE smartphone -------- Original message -------- From: "Gowan,Christie C" Date:03/09/2015 4:01 PM (GMT-05:00) To: 'Bernice Frederick' , histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Old slides. Hi Bernice, I have found that if you flood the slide with mounting media (don't use xylene) flip the slide over onto an absorbent lab wipe and put a heavy weight with even pressure and leave for a few hours. If the slide sticks to the wipe just put a few drops of xylene to clean up the slide. You may still have some tiny bubbles but it is much better than the alternative. Good luck. Christie Gowan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, March 09, 2015 3:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kcastillo <@t> swskin.net Tue Mar 10 13:11:54 2015 From: kcastillo <@t> swskin.net (Kristy Castillo) Date: Tue Mar 10 13:11:58 2015 Subject: [Histonet] PRN Message-ID: Hi Histonetters, I am looking to fill a PRN Histotech position in the Phoenix area. Does anyone know anyone who is retired and looking for PRN Histology work? Please feel free to e-mail me at the following kcastillo@swskin.net Thank you! ________________________________ This transmission may contain confidential information, some or all of which may be protected health information as defined by the federal Health Insurance Portability & Accountability Act (HIPAA) Privacy Rule. This transmission is intended for the exclusive use of the individual or entity to whom it is addressed and may contain information that is proprietary, privileged, confidential and/or exempt from disclosure under applicable law. If you are not the intended recipient (or an employee or agent responsible for delivering this transmission to the intended recipient), you are hereby notified that any disclosure, dissemination, distribution or copying of this information is strictly prohibited and may be subject to legal restriction or sanction. Please notify the sender by telephone to arrange the return or destruction of the information and all copies. From foreightl <@t> gmail.com Tue Mar 10 13:48:23 2015 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Mar 10 13:48:27 2015 Subject: [Histonet] anti-BRAF V600E (VE1) Ventana In-Reply-To: <00bd01d05b55$c09b85f0$41d291d0$@labpath.com> References: <00bd01d05b55$c09b85f0$41d291d0$@labpath.com> Message-ID: Spring Bioscience is part of Ventana, Spring had the original clone, and now so does Ventana. We use the spring bioscience concentrated antibody on the Bond III at 1:100 dilution, using the Bond Hi-pH ER2 retrieval for 20 minutes. I know that Ventana sells the RTU version. Remember that from Spring it is RUO, so it is up to you to do the appropriate LDT. The Ventana antibody is an IVD. It is also a pretty pricey antibody from either vendor. Let me know if you need any further help. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Tue, Mar 10, 2015 at 1:15 PM, Susan Foreman wrote: > What vendor are you guys using for antibody anti-BRAF V600E (VE1)? Spring > Bioscience or Ventana? What dilution are you using? Are you utilizing > amplification? Expensive > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tkngflght <@t> yahoo.com Tue Mar 10 14:37:23 2015 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Mar 10 14:38:10 2015 Subject: [Histonet] Open histology positions: San Francisco Message-ID: <1426016243.35673.YahooMailBasic@web161204.mail.bf1.yahoo.com> Happy Histology Day, everyone! I'm looking for techs for my new (to me) lab!! I'm working at CPMC Sutter in San Francisco and we need a couple of permanent hires for our team. First few days it looks like it's gonna be fun - rock'n'roll kinda place where quality and speed have equal bearing. Let me know what you're looking for and let's see if it's a match! Email resumes to me at the email, below. Please feel free to share and THANK YOU! Cheryl Kerry, HT(ASCP) admin@fullstaff.org From melissa <@t> alliedsearchpartners.com Tue Mar 10 15:28:30 2015 From: melissa <@t> alliedsearchpartners.com (Melissa Owens) Date: Tue Mar 10 15:28:35 2015 Subject: [Histonet] Direct Hire Histotech South of Tucson, AZ-New Grads Welcome to Apply! Message-ID: Hello Histonet, I am in search of a histotech for a permanent position about an hour south of Tucson, AZ. New grads welcome to apply and relocation assistance is offered. Please contact me for more details. Thank you! Melissa Owens Allied Search Partners From thunter <@t> hmc.psu.edu Tue Mar 10 15:43:38 2015 From: thunter <@t> hmc.psu.edu (Hunter, Theresa) Date: Tue Mar 10 15:43:42 2015 Subject: [Histonet] Job opportunity Message-ID: <38012F015BD58743B1EF391E522AD9B2BC025597@EXMBX4.mshmc.local> Hello everyone and happy National Histotechnologist Day! We are looking for a Histology Supervisor to lead a great team in Hershey Pa. If you are interested, please follow the link below. Thanks for your time, Theresa Career Website: http://www.pennstatehershey.org/web/humanresources/home/searchjobs The Penn State Milton S. Hershey Medical Center is committed to affirmative action, equal opportunity and the diversity of its workforce. Equal Opportunity Employer - Minorities/Women/Protected Veterans/Disabled. All individuals (including current employees) selected for a position will undergo a background check appropriate for the position's responsibilities Theresa Hunter, MLS(ASCP)cm Interim Assistant Manager AP Cytology/Decedent care/Histology Lead Pathologist Assistant Department of Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center 500 University Drive, Mail Code H160 Hershey, Pa. 17033 Phone (717) 531-0003 Ext. 287181 Fax (717)531-0831 thunter@hmc.psu.edu From victor_tobias <@t> comcast.net Tue Mar 10 15:50:27 2015 From: victor_tobias <@t> comcast.net (victor_tobias@comcast.net) Date: Tue Mar 10 15:50:43 2015 Subject: [Histonet] TDP43, PTG poly In-Reply-To: <1347048707.1324547.1426020266857.JavaMail.zimbra@comcast.net> Message-ID: <680449490.1325591.1426020627172.JavaMail.zimbra@comcast.net> We stain TDP43 on both FFPE and frozen sections. The FFPE are done on the Bond, but we can't seem to get as good results with the frozen sections on the Bond. Our neuropathologist still prefers manually stained frozen sections for TDP43 over ones done on the Bond. We would really like to get it working reliably on the Bond. ?Does anyone have a protocol they would be willing to share for frozen sections on the Bond? Victor University of Washington Medical Center From ruio7 <@t> hotmail.com Tue Mar 10 19:21:08 2015 From: ruio7 <@t> hotmail.com (Rui TAHARA) Date: Tue Mar 10 19:21:12 2015 Subject: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification In-Reply-To: References: <000601d056d2$8050bc40$80f234c0$@bresnan.net>, , Message-ID: Thank you for all the helpful suggestions about this topic. My sample has been in the fixative until it would be decalcified. Thank you again, rui > From: ree3@leicester.ac.uk > To: ruio7@hotmail.com > Subject: RE: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification > Date: Tue, 10 Mar 2015 09:49:00 +0000 > > Leave it in formalin for as long as possible..........................good luck > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rui TAHARA > Sent: 04 March 2015 23:39 > To: gayle.callis@bresnan.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification > > Thank you for helpful suggestions. > I have further questions. > Yes, I have a bird head (probably 1 cm X 1 cm ) stored in 70 % ethanol. > But i have a similar size bird head fixed in 3.7% formalin for over night and am actually processing the head to store in 70% ethanol since my lab is just ordering the decalcifying solution. I need to decalcify this sample later. > But i am wondering if it is better to keep the sample in formalin for a week or so till i get the decalcification solution or i should store it in 70 % ethanol and then fix it for a few days again later? > I am afraid that longer fixative time would affect the sample somehow (e.g. the sample become too rigid?) > > Thank you, > > rui > > > From: gayle.callis@bresnan.net > > To: histonet@lists.utsouthwestern.edu > > Date: Wed, 4 Mar 2015 16:25:28 -0700 > > Subject: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification > > > > You wrote: > > > > > > > > I have an adult bird skull that fixed with formalin and then has been > > stored in 70% ethanol. > > > > I have seen the post that the sample stored in 70% ethanol can be > > walking back through to series of ethanol to water and can be > > decalcified if it needs to be. > > > > > > > > I am wondering if anybody has done this and there is any side effects > > from decalcification after going through dehydration and rehydration > > of a sample compared to a general straight forward protocol from > > decalcification to dehydration? > > > > ********************************************************************** > > ****** > > ********************************************************************** > > ****** > > ******************************** > > > > > > > > I have, in the past, when a weekend arrive, I interrupted acid bone > > decalcification by removing it from acid decalcifier, a quick water > > rinse and immersed into 70% alcohol before returning bone to fresh > > acid decalcifier the next working day. The bones always decalcified > > without problems but I am sure the decalcification took longer since > > partially decalcified bone had to rehydrate. I later learned more > > about dipolar (hope I said that correctly) alcohol slowing and/or stopping ionization of calcium > > and ceased using 70% alcohol to interrupt acid decalcification. I now use > > NBF to interrupt decalcification. Interestingly, I learned the alcohol > > technique from the AFIP bone pathology lab. > > > > > > > > Alcohol is put into Perenyi's nitric acid decalcifying solutions to slow > > down or control very rapid nitric acid decalcification. > > > > > > > > You did not say how big the bird skull was? I suggest immersing the skull > > back into NBF to let it totally rehydrate for several days (depending > > on skull size and if the brain is present). I suggest changing NBF if you > > rehydrate longer than a day. You don't need to go back through an alcohol > > gradient since many processing schedules have tissue samples going from NBF > > directly into 70%. If you leave residual alcohol in the bones, the acid > > decalcification could be slower and hopefully not retarded in any way. > > > > > > > > > > It certainly is worth a try. Good luck. > > > > > > > > Gayle M. Callis > > > > HTL/HT/MT(ASCP) > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histotalk <@t> yahoo.com Tue Mar 10 11:39:35 2015 From: histotalk <@t> yahoo.com (David Kemler) Date: Tue Mar 10 19:50:21 2015 Subject: [Histonet] BIG day today! Message-ID: <1358663623.2768352.1426005575141.JavaMail.yahoo@mail.yahoo.com> That's right, it's OUR day! NSH is doing their thing. Link here: http://www.nsh.org/content/histotechnology-professionals-day . Yours,Dave From mikeykkk_3 <@t> live.com Tue Mar 10 22:47:25 2015 From: mikeykkk_3 <@t> live.com (Mike) Date: Tue Mar 10 22:47:29 2015 Subject: [Histonet] PA Message-ID: Can you be a pathology assistant with on the job training only? Does it matter if you have a bachelors vs a masters degree in a science not related to pathology or a tech license vs no tech license? Thanks Sent from my iPhone From JMacDonald <@t> mtsac.edu Tue Mar 10 23:17:05 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Mar 10 23:17:13 2015 Subject: [Histonet] PA In-Reply-To: References: Message-ID: To be a PA you would need to have ASCP certification. There is one route to be eligible for certification. To be eligible for this examination category, an applicant must have a minimum of a baccalaureate degree from a regionally accredited college/university, AND successful completion of a NAACLS accredited Pathologists? Assistant program within the last five years. Most of the NAACLS programs are Masters degrees. From: Mike To: "Histonet@lists.utsouthwestern.edu" Date: 03/10/2015 08:49 PM Subject: [Histonet] PA Sent by: histonet-bounces@lists.utsouthwestern.edu Can you be a pathology assistant with on the job training only? Does it matter if you have a bachelors vs a masters degree in a science not related to pathology or a tech license vs no tech license? Thanks Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt <@t> pa-ucl.com Wed Mar 11 07:49:32 2015 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Wed Mar 11 07:49:44 2015 Subject: [Histonet] Diff Quik troubleshooting Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C3601159B7F92@PEITHA.wad.pa-ucl.com> Good Morning- Random FNA case where the slides turn blue as they dry. We have tried taking them back through stains, destaining and restaining, etc. FNA smears are air-dried when we receive them on plain slides, unfixed. Any thoughts appreciated. Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From klaus.dern44 <@t> gmail.com Wed Mar 11 07:52:05 2015 From: klaus.dern44 <@t> gmail.com (Klaus Dern) Date: Wed Mar 11 07:52:08 2015 Subject: [Histonet] THICK AND THIN SECTIONS ? Message-ID: If you are using one of the following microtomes and are being told the advance mechanism is worn out. ( too much play between spindle and spindle nut ) You could be faced with purchasing a new Microtome. ( No parts availability ) REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA/JUNG 2035 LEICA - CM 1850 Cryostat SAKURA SRM 200 Rather than replacing these excellent Instruments, I have a PERMANENT solution to fix this problem. For Information, contact: Klaus Dern Phone: 706 635-8840 E-Mail: klaus.dern44@gmail.com From TNMayer <@t> mdanderson.org Wed Mar 11 10:42:45 2015 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Mar 11 10:43:01 2015 Subject: [Histonet] RE: Old slides Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881D5082D7@D1PWPEXMBX05.mdanderson.edu> Bernice, Take the slide and dip it in xylene. Lay it on the film, pressing down firmly. As it adheres, then gently wipe the excess xylene off, and gently place it in a book or your procedure manual and leave it there for an hour or so. Most of the bubbles will be gone, and the tissue will be saved. The original problem is not enough xylene dispersed onto the slide. Adjust the flow being dispensed by the unit. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Mar 2015 19:41:48 +0000 From: Bernice Frederick Subject: [Histonet] Old slides. To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu From marielachertoff <@t> gmail.com Wed Mar 11 10:53:13 2015 From: marielachertoff <@t> gmail.com (Mariela Chertoff) Date: Wed Mar 11 10:53:18 2015 Subject: [Histonet] 5-methylcytosine IHC in tissue Message-ID: Hi Has anybody experience with 5-metcyt staining in brain tissue? some advice about the HCl and Boric acid treatment? I use 50um free floating sections ferfused with PFA 4%. Thank you in advance for your help!! Mariela Chertoff, PhD Laboratorio de Neuroepigenetica - QB75 Departamento de Qu?mica Biol?gica Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabell?n II Piso 4 Ciudad Aut?noma de Buenos Aires C1428EGA - Argentina Tel: 54 11 4576-3300/09 - Int. 221 email:marielachertoff@gmail.com marielachertoff@qb.fcen.uba.ar From TNMayer <@t> mdanderson.org Wed Mar 11 10:53:12 2015 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Mar 11 10:53:19 2015 Subject: [Histonet] RE: Masson Trichrome stain Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881D508317@D1PWPEXMBX05.mdanderson.edu> Justine, I do not have any metal forceps in the special stains area, due to the reaction that they can cause when staining with silver. As a rule of thumb, it is just easier to use plastic all the way around. The Carson text does not state the use of only plastic forceps, but I would think that maybe they are concerned with a reaction between the Weigert's and the metal. That would be a stretch. As for no water before aniline blue, I believe the concentration is very weak and the water may dilute they dye even further. This would affect the staining results. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 ------------------------------ Message: 4 Date: Tue, 10 Mar 2015 00:31:56 -0500 From: John Kiernan Subject: Re: [Histonet] FW: Masson's trichrome stain To: Linda Margraf , histonet@lists.utsouthwestern.edu Cc: justinelanzon@hotmail.com Message-ID: <7380eaed48941.54fe3b7c@uwo.ca> Content-Type: text/plain; charset=iso-8859-1 The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? John Kiernan = = = On 08/03/15, Linda Margraf wrote: > Here is a message from Justine... > > From: Justine Lanzon [mailto:justinelanzon@hotmail.com] > > Sent: Thursday, March 05, 2015 5:36 AM > To: lindamargraf@gmail.com > Subject: Masson's trichrome stain > > > Hi, > > I am doing a write up on Masson's trichrome stain however I cannot > answer these two questions: > > - Why are plastic forceps used instead of metal ones to hold the > stained slide? > > - Why do we not rinse before Alinine blue? > > ? > > Can you please help me? > > ? > > Many Thanks, > > Justine Lanzon > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ From PAMarcum <@t> uams.edu Wed Mar 11 11:04:35 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Mar 11 11:04:44 2015 Subject: [Histonet] RE: Old slides In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC881D5082D7@D1PWPEXMBX05.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC881D5082D7@D1PWPEXMBX05.mdanderson.edu> Message-ID: <8cf69427ea8f4cdf94fc08543f2d42df@MAIL13M2N2.ad.uams.edu> We have literally about one hundred slides to re-slip for the this reason. Are there any suggestions for large numbers of slides to be re-coverslipped as this method would be too time consuming. We have used only glass for about nine years or so and it is much better. The old ones are the problem when someone needs "THAT" slide only. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Wednesday, March 11, 2015 10:43 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Old slides Bernice, Take the slide and dip it in xylene. Lay it on the film, pressing down firmly. As it adheres, then gently wipe the excess xylene off, and gently place it in a book or your procedure manual and leave it there for an hour or so. Most of the bubbles will be gone, and the tissue will be saved. The original problem is not enough xylene dispersed onto the slide. Adjust the flow being dispensed by the unit. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Mar 2015 19:41:48 +0000 From: Bernice Frederick Subject: [Histonet] Old slides. To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From BSolis <@t> Fibrogen.com Wed Mar 11 11:52:22 2015 From: BSolis <@t> Fibrogen.com (Solis, Bryan) Date: Wed Mar 11 11:52:31 2015 Subject: [Histonet] RE: Histonet Digest, Vol 136, Issue 12 In-Reply-To: <20150310170620.433D434062@barracuda.fibrogen.com> References: <20150310170620.433D434062@barracuda.fibrogen.com> Message-ID: <8c222bc2cfb24928808faf2d0622820a@SF1EX007.fibrogen.com> Hello, We received some liver tissue (Mouse) in 10% formalin (NFB). Then transferred to 70% ETOH. My question is that, Is it ok to transfer to 30% sucrose? So, frozen section can be perform. Please advise. Thanks, Bryan S. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, March 10, 2015 10:06 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 136, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Old slides. (Bernice Frederick) 2. RE: Old slides. (Jason McGough) 3. RE: Mushrooms for GMS fungus control (Morken, Timothy) 4. Re: FW: Masson's trichrome stain (John Kiernan) 5. RE: Old slides. (John Kiernan) 6. soft for microwriter (thermo scientific, Lamb, Shandon) (richard wild) 7. Re: Old slides. (b.curran.mcwilliam@gmail.com) 8. Re: Old slides. (Rene J Buesa) 9. RE: Old slides. (Gowan,Christie C) 10. IHC / Morphometry Technician wanted in Shenandoah Valley Virginia (Erin Sarricks) ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Mar 2015 19:41:48 +0000 From: Bernice Frederick Subject: [Histonet] Old slides. To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu ------------------------------ Message: 2 Date: Mon, 9 Mar 2015 14:20:09 -0600 From: Jason McGough Subject: RE: [Histonet] Old slides. To: Bernice Frederick , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=utf-8 Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgough@clinlab.com www.clinlab.com -----Original message----- > From:Bernice Frederick > > Sent: Monday, March 9, 2015 1:51 PM > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Old slides. > > Hi all, > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. > Thanks, > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ Message: 3 Date: Mon, 9 Mar 2015 22:46:08 +0000 From: "Morken, Timothy" Subject: [Histonet] RE: Mushrooms for GMS fungus control To: "koellingr@comcast.net" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <761E2B5697F795489C8710BCC72141FF367FBA31@ex07.net.ucsf.edu> Content-Type: text/plain; charset="utf-8" Try this article... Acta Cytol. 2003 Nov-Dec;47(6):1043-4. Alternative, cost-effective fungus-staining method for control slides in cytology and histopathology. da Silva VD1. Author information Abstract OBJECTIVE: To develop a cost-effective, reliable and safe method of providing fungal control slides for routine use in pathology laboratories. STUDY DESIGN: A set of easily available, low-cost material was tested to obtain fungal colonies on substrate adequate for paraffin-embedded sections or smears. RESULTS: Such material as cheese is a simple, inexpensive and practical culture medium for silver-positive fungi. A batch of paraffin blocks can be prepared to maintain a stock of control material in the laboratory. CONCLUSION: It is useful to maintain fungal colonies to produce staining control specimens using small pieces of refrigerated cheese to easily produce silver-staining control specimens or smears embedded in paraffin, reducing the risk of accidental exposure to potentially infective pathogens in the laboratory. This method might also be a good alternative for conserving routine surgical specimens, considering the currently decreasing numbers of necropsy and large specimens, particularly from immunosuppressed and infected patients. PMID: 14674076 [PubMed - indexed for MEDLINE] -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Sunday, March 08, 2015 4:29 PM To: Linda Prasad (SCHN) Cc: Jeffrey Robinson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Mushrooms for GMS fungus control Apparently there are numerous interesting ways for fungus or bacteria controls to be had from orange peels to hamburger to slim Jim's to hot dogs to strawberries to ????.?? Sounds like fun to me.?? I'm curious, with the emphasis now on quality control in labs??run amok, has anyone passed a rigorous inspection actually showing these as your currently in-use controls??? A PI in research who??doesn't want??his paper rejected at peer review.?? A CAP inspector in clinical labs who is nit-picky reviewing staining controls but might be looking for a phase anything deficiency.?? The??dot-your-i's and cross-your-t's??FDA people who might or might not OK your drug in development.?? Really, just curious if anyone with a hammer over your head has said it is perfectly fine to use them. Ray, Seattle, WA ----- Original Message ----- From: "Linda Prasad (SCHN)" To: "Jeffrey Robinson" , histonet@lists.utsouthwestern.edu Sent: Sunday, March 8, 2015 4:09:02 PM Subject: [Histonet] RE: Mushrooms for GMS fungus control I used strawberries for a fungal control. Worked really good. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???????Please consider the environment before printing this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Saturday, 7 March 2015 4:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mushrooms for GMS fungus control How about mushrooms? ??Has anyone had any success using mushrooms as a GMS fungus control? Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 10 Mar 2015 00:31:56 -0500 From: John Kiernan Subject: Re: [Histonet] FW: Masson's trichrome stain To: Linda Margraf , histonet@lists.utsouthwestern.edu Cc: justinelanzon@hotmail.com Message-ID: <7380eaed48941.54fe3b7c@uwo.ca> Content-Type: text/plain; charset=iso-8859-1 The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? John Kiernan = = = On 08/03/15, Linda Margraf wrote: > Here is a message from Justine... > > From: Justine Lanzon [mailto:justinelanzon@hotmail.com] > > Sent: Thursday, March 05, 2015 5:36 AM > To: lindamargraf@gmail.com > Subject: Masson's trichrome stain > > > Hi, > > I am doing a write up on Masson's trichrome stain however I cannot > answer these two questions: > > - Why are plastic forceps used instead of metal ones to hold the > stained slide? > > - Why do we not rinse before Alinine blue? > > ? > > Can you please help me? > > ? > > Many Thanks, > > Justine Lanzon > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 5 Date: Tue, 10 Mar 2015 00:40:02 -0500 From: John Kiernan Subject: RE: [Histonet] Old slides. To: Jason McGough , Bernice Frederick , histonet@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu Message-ID: <73e09aa74b442.54fe3d62@uwo.ca> Content-Type: text/plain; charset=iso-8859-1 Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one. John Kiernan Anatomy & Cell Biology, UWO London, Canada = = = On 09/03/15, Jason McGough wrote: > Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. > > > > Jason McGough, HT(ASCP) > > Operations Manager > > Clinical Laboratory of the Black Hills > > 605-343-2267 > > jmcgough@clinlab.com > > > www.clinlab.com > > ? > ? > -----Original message----- > > From:Bernice Frederick > > > > > > > Sent: Monday, March 9, 2015 1:51 PM > > To: histonet@lists.utsouthwestern.edu > > > > > > Subject: [Histonet] Old slides. > > > > Hi all, > > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 6 Date: Tue, 10 Mar 2015 10:05:11 +0100 From: richard wild Subject: [Histonet] soft for microwriter (thermo scientific, Lamb, Shandon) To: histonet@lists.utsouthwestern.edu Message-ID: <54FEB3C7.40306@wanadoo.fr> Content-Type: text/plain; charset=utf-8; format=flowed Hi I am looking for labelling software (or advices) for the carousel microwriter (thermo scientific, Lamb, Shandon = same machine) (LAMB E22.01MWR) The machine is discontinuated. I would like to use serial interface rs232 and barcode scanners. Thanks for help. Richard ------------------------------ Message: 7 Date: Tue, 10 Mar 2015 11:51:25 +0000 From: b.curran.mcwilliam@gmail.com Subject: Re: [Histonet] Old slides. To: Bernice Frederick Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <9FA4B5FB-11BE-47C7-AA0C-6F8416B10487@gmail.com> Content-Type: text/plain; charset=us-ascii Hi We re-Coverslipper a number of sections which had peeled off on the tape as the tape dried and curled. We cut off excess tape using scissors; placed a fresh coverslip flat; put a streak of mounting medium on he coverslip; use a forceps to orientate the tissue + margin of tape; number a slide, dip it in Xylene & place on a slope & bring on top of coverslip-section-mounting medium; turn the slide-section- coverslip to face coverslip up; leave horizontal to dry (eg overnight). Worth a try, doing one first. Bernie, St Vincent's, Dublin, Ireland > On 9 Mar 2015, at 19:41, Bernice Frederick wrote: > > Hi all, > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. > Thanks, > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 10 Mar 2015 13:43:35 +0000 (UTC) From: Rene J Buesa Subject: Re: [Histonet] Old slides. To: John Kiernan , Jason McGough , Bernice Frederick , "histonet@lists.utsouthwestern.edu" Message-ID: <1897656965.1753669.1425995015431.JavaMail.yahoo@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 To John Probably the writer is referring to slides "mounted" with the Sakura film??"coverslip".I have done it many times and the FILM will??detach easily from the section.Had it been a glass coverslip attached with, for example, Permount, acetone would not have done anything.Ren?? J.?? On Tuesday, March 10, 2015 1:40 AM, John Kiernan wrote: Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one. John Kiernan Anatomy & Cell Biology, UWO London, Canada = = = On 09/03/15, Jason McGough?? wrote: > Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. > > > > Jason McGough, HT(ASCP) > > Operations Manager > > Clinical Laboratory of the Black Hills > > 605-343-2267 > > jmcgough@clinlab.com > > > www.clinlab.com > > ?? > ?? > -----Original message----- > > From:Bernice Frederick > > > > Sent: Monday, March 9, 2015 1:51 PM > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] Old slides. > > > > Hi all, > > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 9 Mar 2015 20:01:10 +0000 From: "Gowan,Christie C" Subject: [Histonet] RE: Old slides. To: "'Bernice Frederick'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Bernice, I have found that if you flood the slide with mounting media (don't use xylene) flip the slide over onto an absorbent lab wipe and put a heavy weight with even pressure and leave for a few hours. If the slide sticks to the wipe just put a few drops of xylene to clean up the slide. You may still have some tiny bubbles but it is much better than the alternative. Good luck. Christie Gowan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, March 09, 2015 3:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 10 Mar 2015 12:12:05 -0400 From: Erin Sarricks Subject: [Histonet] IHC / Morphometry Technician wanted in Shenandoah Valley Virginia To: histonet Message-ID: Content-Type: text/plain; charset=UTF-8 Histology Laboratory located in the Shenandoah Valley of Virginia is looking to add to it's team. In this position, you will need a working knowledge of IHC theory and practical IHC experience. The best candidate for the position will oversee immunohistochemical staining as well as perform other histology functions including trimming of specimens, paraffin embedding, microtomy and microscopic QC of slides. Experience with morphometry is preferable. Desirable candidates will possess the following: - HT (ASCP) or QIHC registration preferred - 4 years of Histology experience - 1+ years of immunohistochemistry and/or immunofluorescence experience - Keeps abreast with company's current policies and immunohistochemistry technical updates and procedures - Must be able to work independently and in a team environment Full time employment benefits include subsidized medical and dental insurance, vacation, holiday pay, and 401k after 1 year of employment. Compensation is commensurate with experience. If you are interested in this position, please respond to this post with your resume and cover letter. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 136, Issue 12 ***************************************** From patrick.lewis <@t> seattlechildrens.org Wed Mar 11 12:51:42 2015 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Wed Mar 11 12:51:48 2015 Subject: [Histonet] Acetone fixing and tissue damage Message-ID: <3903BE18914F4440834F0E620415FFCA3CB5D55F@PPWEXD01d.childrens.sea.kids> Hi Everyone. When I fix my cryosections in acetone, I am using HPLC grade 99.9% for 10 minutes at -20C. Would the Histology grade 99.5% be less damaging to them? Higher H20 content, i.e. less than 99.5% apparently is also very bad. With the HPLC grade I often get tissue damage, the tissue also floats off the slide causing a stringy effect. Fixing with 4% p-formaldehyde or 100% Methanol, prevented the antibody from recognizing the Nuclear Antigens. Looking for advice, Patrick. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From bethany <@t> mydermsurgery.com Wed Mar 11 13:59:48 2015 From: bethany <@t> mydermsurgery.com (bethany@mydermsurgery.com) Date: Wed Mar 11 13:59:57 2015 Subject: [Histonet] repair 350-2 Thermo Scientific Message-ID: The cyro console on our 350-2 Thermo Scientific is no longer cooling. Any ideas about getting this repaired and/or replaced?? We are located in central Florida. Thx From patpxs <@t> gmail.com Thu Mar 12 08:29:18 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Thu Mar 12 08:29:21 2015 Subject: [Histonet] Embedding Question Message-ID: It has been proposed to move the embedding centers to a room about 210 ft away from the tissue processors. The trip from processor to embedding center would take over 2 minutes and require the histotechs to carry the baskets full of cassettes down a much used hallway. Opinions? Do you feel this is a good idea-yes or no and why? Thanks in advance, Paula From mucram11 <@t> comcast.net Thu Mar 12 08:41:24 2015 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Thu Mar 12 08:41:40 2015 Subject: [Histonet] Embedding Question In-Reply-To: References: Message-ID: <221887849.835927.1426167684782.JavaMail.zimbra@comcast.net> I am sorry anytime you are carrying patient tissue in cassettes (processed or not) is a bad plan.? In order to guarantee the tissue is safe it would need to be enclosed in a way that an accidental bump or fall could not allow tissue cassettes to go flying off down the hall.? Playing 52 card pick up with cassettes is not my idea of fun and not finding one or two could result in a bad diagnosis if no other tissue was available to replace it.? Biopsies come to mind immediately.? ? Since you noted it is a busy hall you also have the risk of paraffin falling on the floor and causing someone else to fall, which should be a lawsuit waiting to happen.? The inconvenience is out weight by the possible incidents this could cause involving patient tissues, hospital personnel and visitors to your facility.? All of this plus adding time to carry baskets to another area and back for cleaning and use. ? Pam Marcum UAMS ----- Original Message ----- From: "Paula Sicurello" To: "Histonet" Sent: Thursday, March 12, 2015 8:29:18 AM Subject: [Histonet] Embedding Question It has been proposed to move the embedding centers to a room about 210 ft away from the tissue processors. The trip from processor to embedding center would take over 2 minutes and require the histotechs to carry the baskets full of cassettes down a much used hallway. Opinions? Do you feel this is a good idea-yes or no and why? Thanks in advance, Paula _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Mar 12 09:27:45 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 12 09:27:50 2015 Subject: [Histonet] Embedding Question In-Reply-To: References: Message-ID: <238119038.3313648.1426170465873.JavaMail.yahoo@mail.yahoo.com> Whom ever made the decision (probably an "administrator") is totally ignorant of what histology flow is all about.To put it "mildly" it is an absolutely stupid decision.Has this "decider" any idea about "Lean", sure not.To have a good work flow everything should be as close as possible.Ren? J.? On Thursday, March 12, 2015 9:29 AM, Paula Sicurello wrote: It has been proposed to move the embedding centers to a room about 210 ft away from the tissue processors. The trip from processor to embedding center would take over 2 minutes and require the histotechs to carry the baskets full of cassettes down a much used hallway. Opinions? Do you feel this is a good idea-yes or no and why? Thanks in advance, Paula _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Thu Mar 12 09:31:37 2015 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Thu Mar 12 09:31:56 2015 Subject: [Histonet] Embedding Question In-Reply-To: References: Message-ID: <38667E7FB77ECD4E91BFAEB8D98638632616513FAF@LRGHEXVS1.practice.lrgh.org> Not what I call a lean system. Why the change? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Thursday, March 12, 2015 9:29 AM To: HistoNet Subject: [Histonet] Embedding Question It has been proposed to move the embedding centers to a room about 210 ft away from the tissue processors. The trip from processor to embedding center would take over 2 minutes and require the histotechs to carry the baskets full of cassettes down a much used hallway. Opinions? Do you feel this is a good idea-yes or no and why? Thanks in advance, Paula _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From patpxs <@t> gmail.com Thu Mar 12 09:43:03 2015 From: patpxs <@t> gmail.com (Patpxs) Date: Thu Mar 12 09:43:12 2015 Subject: [Histonet] Embedding Question In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638632616513FAF@LRGHEXVS1.practice.lrgh.org> References: <38667E7FB77ECD4E91BFAEB8D98638632616513FAF@LRGHEXVS1.practice.lrgh.org> Message-ID: <12119F8E-15F9-402F-86F0-B87BF542B520@gmail.com> Why the change? Space. There is a larger space available for histology. I feel they should either move the processors into the new space or leave the embedding centers where they are now. I mentioned did bring up about the samples/wax freezing in transit and the reply was "the experts didn't mention anything about that" I think the experts were not experienced histotechs. Paula Sent from my iPhone > On Mar 12, 2015, at 7:31 AM, Podawiltz, Thomas wrote: > > Not what I call a lean system. Why the change? > > Tom > > > Tom Podawiltz HT (ASCP) > AP Section Head > LRGHealthcare > 603-524-3211 ext: 3220 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > Sent: Thursday, March 12, 2015 9:29 AM > To: HistoNet > Subject: [Histonet] Embedding Question > > It has been proposed to move the embedding centers to a room about 210 ft away from the tissue processors. > > The trip from processor to embedding center would take over 2 minutes and require the histotechs to carry the baskets full of cassettes down a much used hallway. > > Opinions? > > Do you feel this is a good idea-yes or no and why? > > Thanks in advance, > > Paula > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From Erin.Martin <@t> ucsf.edu Thu Mar 12 10:03:26 2015 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Thu Mar 12 10:17:06 2015 Subject: [Histonet] Re: Old slides Message-ID: <24B7B291CC88D04AB663958E77A1F59D1E4991@ex09.net.ucsf.edu> Hi Bernice! A 1:1 acetone xylene solution does indeed dissolve the plastic film Sakura coverslip. No need to buy any book. The old - 1990's - film coverslips had a problem (that I believe Sakura has corrected) which caused the coverslip to lift off the slide and take the tissue with it. That's why there are often issues with film coverslipped slides from that time period. It's a pain to deal with but not difficult. Good luck! Erin Martin UCSF Dermatopathology and Oral Pathology Service Message: 5 >On Tue, 10 Mar 2015 John Kiernan jkiernan@uwo.ca wrote: >Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all >in the books; buy one. >John Kiernan >Anatomy & Cell Biology, UWO >London, Canada Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. From billodonnell <@t> catholichealth.net Thu Mar 12 10:18:52 2015 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Thu Mar 12 10:19:23 2015 Subject: [Histonet] Embedding Question In-Reply-To: References: Message-ID: Wow. Speechless. (well nearly speechless) Wow. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Thursday, March 12, 2015 8:29 AM To: HistoNet Subject: [Histonet] Embedding Question It has been proposed to move the embedding centers to a room about 210 ft away from the tissue processors. The trip from processor to embedding center would take over 2 minutes and require the histotechs to carry the baskets full of cassettes down a much used hallway. Opinions? Do you feel this is a good idea-yes or no and why? Thanks in advance, Paula _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIBaQ&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=3iEA9oByFKGUVmHHjEGViq9-hi91LvAPvOKSxbQaAak&m=Z1oOrSS6RzAgCDvKpzUacQVCuoAJ8m8HWh5TAjVZEl0&s=gFaW1bvH7xHTQ8IRNT5DF0Fu2a-YqZVKp3V0JjWc_XA&e= This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From Rhonda.Gregoire <@t> gov.mb.ca Thu Mar 12 10:21:09 2015 From: Rhonda.Gregoire <@t> gov.mb.ca (Gregoire, Rhonda (MAFRD)) Date: Thu Mar 12 10:21:30 2015 Subject: [Histonet] Eosinophil staining with AFB stain? Message-ID: Could anyone tell me if eosinophils will stain up with AFB stain? We are using the Richard Allen Scientific kit. Thanks Rhonda Gregoire Veterinary Diagnostic Services Winnipeg, Manitoba Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email Rhonda.Gregoire@gov.mb.ca From Timothy.Morken <@t> ucsf.edu Thu Mar 12 11:07:29 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Thu Mar 12 11:07:43 2015 Subject: [Histonet] Embedding Question In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF367FC4D2@ex07.net.ucsf.edu> Paula, How many times per day? Is the embedding close to the cutting area? Of course any extra walking is a problem, especially in busy areas. Is this a non-patient area (hopefully!)? Any restructuring should be to move things closer together, not further away! Having said that, If it comes to that I would be more concerned about embedding proximity to the cutting area since having embedding near cutting enhances workflow and cross coverage. If you don't unload processors very often then having them distant might not be too bad. Not ideal, but not a necessarily a deal killer. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Thursday, March 12, 2015 6:29 AM To: HistoNet Subject: [Histonet] Embedding Question It has been proposed to move the embedding centers to a room about 210 ft away from the tissue processors. The trip from processor to embedding center would take over 2 minutes and require the histotechs to carry the baskets full of cassettes down a much used hallway. Opinions? Do you feel this is a good idea-yes or no and why? Thanks in advance, Paula _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen <@t> parrishmed.com Thu Mar 12 09:56:52 2015 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Thu Mar 12 11:41:47 2015 Subject: [Histonet] Embedding Question In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638632616513FAF@LRGHEXVS1.practice.lrgh.org> References: <38667E7FB77ECD4E91BFAEB8D98638632616513FAF@LRGHEXVS1.practice.lrgh.org> Message-ID: <450B7A81EDA0C54E97C53D60F00776C3233738422B@isexstore03> I do not think it to be a good idea. 1) What is the possibility of a "trip and fall" in transit? Cassettes flying everywhere, possibility of losing any? 2) Dripping paraffin on a hallway floor-- a slip and fall scenario for other people using the "much used" hallway. 3) As Tom stated.. not a lean system/process. Just my two cents! Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, March 12, 2015 10:32 AM To: Paula Sicurello; HistoNet Subject: RE: [Histonet] Embedding Question Not what I call a lean system. Why the change? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Thursday, March 12, 2015 9:29 AM To: HistoNet Subject: [Histonet] Embedding Question It has been proposed to move the embedding centers to a room about 210 ft away from the tissue processors. The trip from processor to embedding center would take over 2 minutes and require the histotechs to carry the baskets full of cassettes down a much used hallway. Opinions? Do you feel this is a good idea-yes or no and why? Thanks in advance, Paula _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From patpxs <@t> gmail.com Thu Mar 12 11:43:51 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Thu Mar 12 11:44:01 2015 Subject: [Histonet] Embedding Question In-Reply-To: <761E2B5697F795489C8710BCC72141FF367FC4D2@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367FC4D2@ex07.net.ucsf.edu> Message-ID: Hi Tim, There are several embedding events through-out the day, though mostly in the wee hours of the morning. The embedding centers would be in the same room as the microtomes (another question about those tomorrow). I worry about the small (GI, needles, etc) biopsies freezing before they reach the embedding stations. In my experience, once they freeze they get this outer wax coating (like a permeability barrier) which doesn't melt when placed in the dry (no paraffin inside) but hot, holding bin. They just don't seem to embed that well and have a tendency to drop out of the sections when cutting. Has anyone else had that happen? Paula On Thu, Mar 12, 2015 at 9:07 AM, Morken, Timothy wrote: > Paula, > How many times per day? > Is the embedding close to the cutting area? > > Of course any extra walking is a problem, especially in busy areas. Is > this a non-patient area (hopefully!)? Any restructuring should be to move > things closer together, not further away! > > Having said that, If it comes to that I would be more concerned about > embedding proximity to the cutting area since having embedding near cutting > enhances workflow and cross coverage. If you don't unload processors very > often then having them distant might not be too bad. Not ideal, but not a > necessarily a deal killer. > > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > Sent: Thursday, March 12, 2015 6:29 AM > To: HistoNet > Subject: [Histonet] Embedding Question > > It has been proposed to move the embedding centers to a room about 210 ft > away from the tissue processors. > > The trip from processor to embedding center would take over 2 minutes and > require the histotechs to carry the baskets full of cassettes down a much > used hallway. > > Opinions? > > Do you feel this is a good idea-yes or no and why? > > Thanks in advance, > > Paula > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From patpxs <@t> gmail.com Thu Mar 12 11:46:56 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Thu Mar 12 11:47:00 2015 Subject: [Histonet] Embedding Question In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C3233738422B@isexstore03> References: <38667E7FB77ECD4E91BFAEB8D98638632616513FAF@LRGHEXVS1.practice.lrgh.org> <450B7A81EDA0C54E97C53D60F00776C3233738422B@isexstore03> Message-ID: Why the change? Space. There is a larger space available for histology. It would be optimum to either move the processors into the new space or leave the embedding centers where they are now. I mentioned did bring up about the samples/wax freezing in transit and the reply was "the experts didn't mention anything about that" The experts may not have been experienced histotechs. Paula On Thu, Mar 12, 2015 at 7:56 AM, Hannen, Valerie < Valerie.Hannen@parrishmed.com> wrote: > I do not think it to be a good idea. > > 1) What is the possibility of a "trip and fall" in transit? Cassettes > flying everywhere, possibility of losing any? > > 2) Dripping paraffin on a hallway floor-- a slip and fall scenario for > other people using the "much used" hallway. > > 3) As Tom stated.. not a lean system/process. > > Just my two cents! > > > Valerie Hannen,MLT(ASCP),HTL,SU (FL) > Section Chief, Histology > Parrish Medical Center > 951 N. Washington Ave. > Titusville,Florida 32796 > T: (321)268-6333 ext. 7506 > F: (321) 268-6149 > valerie.hannen@parrishmed.com > www.parrishmed.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas > Sent: Thursday, March 12, 2015 10:32 AM > To: Paula Sicurello; HistoNet > Subject: RE: [Histonet] Embedding Question > > Not what I call a lean system. Why the change? > > Tom > > > Tom Podawiltz HT (ASCP) > AP Section Head > LRGHealthcare > 603-524-3211 ext: 3220 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > Sent: Thursday, March 12, 2015 9:29 AM > To: HistoNet > Subject: [Histonet] Embedding Question > > It has been proposed to move the embedding centers to a room about 210 ft > away from the tissue processors. > > The trip from processor to embedding center would take over 2 minutes and > require the histotechs to carry the baskets full of cassettes down a much > used hallway. > > Opinions? > > Do you feel this is a good idea-yes or no and why? > > Thanks in advance, > > Paula > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential > information intended only for the use of the recipient(s) named above. If > you are not the intended recipient, you may not print,distribute, or copy > this message or any attachments. If you have received this communication > in error, please notify the sender by return e-mail and delete this message > and any attachments from your computer. Any views or opinions expressed are > solely those of the author and do not necessarily represent those of > LRGHealthcare. > > ====================================== > "This email is intended solely for the use of the individual to > whom it is addressed and may contain information that is > privileged, confidential or otherwise exempt from disclosure > under applicable law. If the reader of this email is not the > intended recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you > have received this communication in error, please immediately > delete this message. Thank you" > ====================================== > From gayle.callis <@t> bresnan.net Thu Mar 12 12:02:21 2015 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Mar 12 12:02:28 2015 Subject: [Histonet] Acetone fixing and tissue damage Message-ID: <000001d05ce6$4e899fa0$eb9cdee0$@bresnan.net> You wrote: Hi Everyone. When I fix my cryosections in acetone, I am using HPLC grade 99.9% for 10 minutes at -20C. Would the Histology grade 99.5% be less damaging to them? Higher H20 content, i.e. less than 99.5% apparently is also very bad. With the HPLC grade I often get tissue damage, the tissue also floats off the slide causing a stringy effect. Fixing with 4% p-formaldehyde or 100% Methanol, prevented the antibody from recognizing the Nuclear Antigens. Looking for advice, Patrick. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 **************************************************************************** ********************* HPLC grade acetone is not necessary plus very expensive. Use ACS Certified Reagent 99.5% grade, not histology grade, which you can buy in gallon size. Maybe what you are calling histology grade is the ACS Certified Reagent grade but "Histology" grade implies a practical grade of acetone which is not a pure as the ACS certified Reagent grade. Also, 4?C acetone works just as well. If you are storing your acetone (in a staining jar) inside the cryostat to maintain a 20?C temperature, don't!!! If your staining container tips over, you will ruin your cryostat!! Hopefully you are using high quality plus charge slides? We had frozen sections come off a plus charge slide after single 4?C acetone fixation on occasion. You can prevent frozen section loss is a Double Acetone fixation that also increases permeabilization. An IHC guru gave me this hint years ago and was given to her by a company selling immunostaining products. A small fan will be your best friend for RT air drying and/or evaporating acetone. However we air dried all FS were dried at RT for 30 minutes minimum or longer before fixation. By air drying, you get rid of the water. You can put your FS in front of a small fan, or inside a hood for faster drying, and never store just cut FS in the cryostat where water condensation occurs when you take them out of cryostat environment to RT. DRY frozen sections were the rule in our lab before any solvent fixation. 1. Air dry frozen section at RT for 30 min 2. Fix FS in 4?C acetone for 10 minutes 3. Air dry FS for 15 minutes to evaporate acetone 4. Return FS to 4?C acetone for 10 minutes 5. Air dry to evaporate acetone 6. Proceed to immunostaining Gayle Callis HTL/HT/MT(ASCP) From lguernsey <@t> ucsd.edu Thu Mar 12 12:08:20 2015 From: lguernsey <@t> ucsd.edu (Lucie Guernsey) Date: Thu Mar 12 12:09:05 2015 Subject: [Histonet] Embedding Question In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF367FC4D2@ex07.net.ucsf.edu> Message-ID: If I may, I'd like to piggy-back onto what Paula has mentioned regarding allowing paraffin infiltrated tissue to cool before embedding it. Hopefully someone can help both of us out, even if we seem to warm our infiltrated tissue differently (Paula's in a dry bin and mine in a wax bath). I work in a research lab where we work in large batches and time is not a priority like it is in a clinical setting. Rather than leaving 60-80 cassettes of infiltrated tissue soaking in a hot wax bath for hours at a time, we've begun to allow the cassettes to cool and just toss a handful of cassettes into the wax bath 5-10 minutes before we're ready to embed that batch of cassettes. Sometimes we don't even embed the cooled tissue until the next day or later that week. I haven't noticed an obvious difference in how our blocks section, though we have troublesome batches sometimes and we haven't been able to put our finger on why. Anyone know if allowing infiltrated tissue to cool and then reheat before embedding is better or worse than keeping the tissue soaking in wax for hours at a time? Thanks! Lucie Lucie Guernsey UC San Diego lguernsey@ucsd.edu On Thu, Mar 12, 2015 at 9:43 AM, Paula Sicurello wrote: > Hi Tim, > > There are several embedding events through-out the day, though mostly in > the wee hours of the morning. The embedding centers would be in the same > room as the microtomes (another question about those tomorrow). > > I worry about the small (GI, needles, etc) biopsies freezing before they > reach the embedding stations. In my experience, once they freeze they get > this outer wax coating (like a permeability barrier) which doesn't melt > when placed in the dry (no paraffin inside) but hot, holding bin. > > They just don't seem to embed that well and have a tendency to drop out of > the sections when cutting. > > Has anyone else had that happen? > > Paula > > On Thu, Mar 12, 2015 at 9:07 AM, Morken, Timothy > wrote: > > > Paula, > > How many times per day? > > Is the embedding close to the cutting area? > > > > Of course any extra walking is a problem, especially in busy areas. Is > > this a non-patient area (hopefully!)? Any restructuring should be to move > > things closer together, not further away! > > > > Having said that, If it comes to that I would be more concerned about > > embedding proximity to the cutting area since having embedding near > cutting > > enhances workflow and cross coverage. If you don't unload processors very > > often then having them distant might not be too bad. Not ideal, but not a > > necessarily a deal killer. > > > > > > Tim Morken > > Pathology Site Manager, Parnassus > > Supervisor, Electron Microscopy/Neuromuscular Special Studies > > Department of Pathology > > UC San Francisco Medical Center > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > > Sent: Thursday, March 12, 2015 6:29 AM > > To: HistoNet > > Subject: [Histonet] Embedding Question > > > > It has been proposed to move the embedding centers to a room about 210 ft > > away from the tissue processors. > > > > The trip from processor to embedding center would take over 2 minutes and > > require the histotechs to carry the baskets full of cassettes down a much > > used hallway. > > > > Opinions? > > > > Do you feel this is a good idea-yes or no and why? > > > > Thanks in advance, > > > > Paula > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From wdesalvo.cac <@t> outlook.com Thu Mar 12 12:22:19 2015 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Thu Mar 12 12:22:27 2015 Subject: [Histonet] Embedding Question In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF367FC4D2@ex07.net.ucsf.edu> Message-ID: Cooling the paraffin and then re melting will not affect the tissue, unless the combined heated, liquified state period becomes extended. Cooling the paraffin is a great protector of the tissue, no different than what you have with a completed block. Be cautious at how fast and at what temperature you reheat at. With dry embedding, you have to be cautious about small tissue pieces obtaining a drying or heat affect. The small tissue pieces are typically at the bottom of the cassette and closest to the heating element, without the insulation of the liquid paraffin. Wet embedding there is a possibility of debris tissue fragments floating amongst the cassette. Both methods require cleanliness and short times in liquid paraffin to embedding. Sent from my iPhone > On Mar 12, 2015, at 10:09 AM, Lucie Guernsey wrote: > > If I may, I'd like to piggy-back onto what Paula has mentioned regarding > allowing paraffin infiltrated tissue to cool before embedding it. Hopefully > someone can help both of us out, even if we seem to warm our infiltrated > tissue differently (Paula's in a dry bin and mine in a wax bath). > > I work in a research lab where we work in large batches and time is not a > priority like it is in a clinical setting. Rather than leaving 60-80 > cassettes of infiltrated tissue soaking in a hot wax bath for hours at a > time, we've begun to allow the cassettes to cool and just toss a handful of > cassettes into the wax bath 5-10 minutes before we're ready to embed that > batch of cassettes. Sometimes we don't even embed the cooled tissue until > the next day or later that week. I haven't noticed an obvious difference in > how our blocks section, though we have troublesome batches sometimes and we > haven't been able to put our finger on why. > > Anyone know if allowing infiltrated tissue to cool and then reheat before > embedding is better or worse than keeping the tissue soaking in wax for > hours at a time? > > Thanks! > Lucie > > Lucie Guernsey > UC San Diego > lguernsey@ucsd.edu > > > >> On Thu, Mar 12, 2015 at 9:43 AM, Paula Sicurello wrote: >> >> Hi Tim, >> >> There are several embedding events through-out the day, though mostly in >> the wee hours of the morning. The embedding centers would be in the same >> room as the microtomes (another question about those tomorrow). >> >> I worry about the small (GI, needles, etc) biopsies freezing before they >> reach the embedding stations. In my experience, once they freeze they get >> this outer wax coating (like a permeability barrier) which doesn't melt >> when placed in the dry (no paraffin inside) but hot, holding bin. >> >> They just don't seem to embed that well and have a tendency to drop out of >> the sections when cutting. >> >> Has anyone else had that happen? >> >> Paula >> >> On Thu, Mar 12, 2015 at 9:07 AM, Morken, Timothy >> wrote: >> >>> Paula, >>> How many times per day? >>> Is the embedding close to the cutting area? >>> >>> Of course any extra walking is a problem, especially in busy areas. Is >>> this a non-patient area (hopefully!)? Any restructuring should be to move >>> things closer together, not further away! >>> >>> Having said that, If it comes to that I would be more concerned about >>> embedding proximity to the cutting area since having embedding near >> cutting >>> enhances workflow and cross coverage. If you don't unload processors very >>> often then having them distant might not be too bad. Not ideal, but not a >>> necessarily a deal killer. >>> >>> >>> Tim Morken >>> Pathology Site Manager, Parnassus >>> Supervisor, Electron Microscopy/Neuromuscular Special Studies >>> Department of Pathology >>> UC San Francisco Medical Center >>> >>> >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >>> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello >>> Sent: Thursday, March 12, 2015 6:29 AM >>> To: HistoNet >>> Subject: [Histonet] Embedding Question >>> >>> It has been proposed to move the embedding centers to a room about 210 ft >>> away from the tissue processors. >>> >>> The trip from processor to embedding center would take over 2 minutes and >>> require the histotechs to carry the baskets full of cassettes down a much >>> used hallway. >>> >>> Opinions? >>> >>> Do you feel this is a good idea-yes or no and why? >>> >>> Thanks in advance, >>> >>> Paula >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Thu Mar 12 12:35:39 2015 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Thu Mar 12 12:35:46 2015 Subject: [Histonet] Embedding Question In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF367FC4D2@ex07.net.ucsf.edu> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638632616513FD7@LRGHEXVS1.practice.lrgh.org> Well said. As a tech who once dropped a tray of cassettes on the floor I would be more concerned about the travel from the processors to the embedding unit. Just imagine a bunch of gastric biopsy cassettes scattered down the hallway and one or two open cassettes on the floor. Increase the travel time and distance and increase the risk. Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare 603-524-3211 ext: 3220 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Thursday, March 12, 2015 1:22 PM To: Lucie Guernsey Cc: HistoNet; Morken, Timothy Subject: Re: [Histonet] Embedding Question Cooling the paraffin and then re melting will not affect the tissue, unless the combined heated, liquified state period becomes extended. Cooling the paraffin is a great protector of the tissue, no different than what you have with a completed block. Be cautious at how fast and at what temperature you reheat at. With dry embedding, you have to be cautious about small tissue pieces obtaining a drying or heat affect. The small tissue pieces are typically at the bottom of the cassette and closest to the heating element, without the insulation of the liquid paraffin. Wet embedding there is a possibility of debris tissue fragments floating amongst the cassette. Both methods require cleanliness and short times in liquid paraffin to embedding. Sent from my iPhone > On Mar 12, 2015, at 10:09 AM, Lucie Guernsey wrote: > > If I may, I'd like to piggy-back onto what Paula has mentioned > regarding allowing paraffin infiltrated tissue to cool before > embedding it. Hopefully someone can help both of us out, even if we > seem to warm our infiltrated tissue differently (Paula's in a dry bin and mine in a wax bath). > > I work in a research lab where we work in large batches and time is > not a priority like it is in a clinical setting. Rather than leaving > 60-80 cassettes of infiltrated tissue soaking in a hot wax bath for > hours at a time, we've begun to allow the cassettes to cool and just > toss a handful of cassettes into the wax bath 5-10 minutes before > we're ready to embed that batch of cassettes. Sometimes we don't even > embed the cooled tissue until the next day or later that week. I > haven't noticed an obvious difference in how our blocks section, > though we have troublesome batches sometimes and we haven't been able to put our finger on why. > > Anyone know if allowing infiltrated tissue to cool and then reheat > before embedding is better or worse than keeping the tissue soaking in > wax for hours at a time? > > Thanks! > Lucie > > Lucie Guernsey > UC San Diego > lguernsey@ucsd.edu > > > >> On Thu, Mar 12, 2015 at 9:43 AM, Paula Sicurello wrote: >> >> Hi Tim, >> >> There are several embedding events through-out the day, though mostly >> in the wee hours of the morning. The embedding centers would be in >> the same room as the microtomes (another question about those tomorrow). >> >> I worry about the small (GI, needles, etc) biopsies freezing before >> they reach the embedding stations. In my experience, once they >> freeze they get this outer wax coating (like a permeability barrier) >> which doesn't melt when placed in the dry (no paraffin inside) but hot, holding bin. >> >> They just don't seem to embed that well and have a tendency to drop >> out of the sections when cutting. >> >> Has anyone else had that happen? >> >> Paula >> >> On Thu, Mar 12, 2015 at 9:07 AM, Morken, Timothy >> >> wrote: >> >>> Paula, >>> How many times per day? >>> Is the embedding close to the cutting area? >>> >>> Of course any extra walking is a problem, especially in busy areas. >>> Is this a non-patient area (hopefully!)? Any restructuring should be >>> to move things closer together, not further away! >>> >>> Having said that, If it comes to that I would be more concerned >>> about embedding proximity to the cutting area since having embedding >>> near >> cutting >>> enhances workflow and cross coverage. If you don't unload processors >>> very often then having them distant might not be too bad. Not ideal, >>> but not a necessarily a deal killer. >>> >>> >>> Tim Morken >>> Pathology Site Manager, Parnassus >>> Supervisor, Electron Microscopy/Neuromuscular Special Studies >>> Department of Pathology UC San Francisco Medical Center >>> >>> >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu [mailto: >>> histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula >>> Sicurello >>> Sent: Thursday, March 12, 2015 6:29 AM >>> To: HistoNet >>> Subject: [Histonet] Embedding Question >>> >>> It has been proposed to move the embedding centers to a room about >>> 210 ft away from the tissue processors. >>> >>> The trip from processor to embedding center would take over 2 >>> minutes and require the histotechs to carry the baskets full of >>> cassettes down a much used hallway. >>> >>> Opinions? >>> >>> Do you feel this is a good idea-yes or no and why? >>> >>> Thanks in advance, >>> >>> Paula >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From b-frederick <@t> northwestern.edu Thu Mar 12 12:48:56 2015 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Thu Mar 12 12:49:02 2015 Subject: [Histonet] Acetone fixing and tissue damage In-Reply-To: <000001d05ce6$4e899fa0$eb9cdee0$@bresnan.net> References: <000001d05ce6$4e899fa0$eb9cdee0$@bresnan.net> Message-ID: We use a 3:1 ration of cold acetone/100% ETOH to fix frozens for IHC. Slides are always air dried first to remove any moisture. Usually 75 mls acetone,25 mls alcohol. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, March 12, 2015 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Acetone fixing and tissue damage You wrote: Hi Everyone. When I fix my cryosections in acetone, I am using HPLC grade 99.9% for 10 minutes at -20C. Would the Histology grade 99.5% be less damaging to them? Higher H20 content, i.e. less than 99.5% apparently is also very bad. With the HPLC grade I often get tissue damage, the tissue also floats off the slide causing a stringy effect. Fixing with 4% p-formaldehyde or 100% Methanol, prevented the antibody from recognizing the Nuclear Antigens. Looking for advice, Patrick. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 **************************************************************************** ********************* HPLC grade acetone is not necessary plus very expensive. Use ACS Certified Reagent 99.5% grade, not histology grade, which you can buy in gallon size. Maybe what you are calling histology grade is the ACS Certified Reagent grade but "Histology" grade implies a practical grade of acetone which is not a pure as the ACS certified Reagent grade. Also, 4?C acetone works just as well. If you are storing your acetone (in a staining jar) inside the cryostat to maintain a 20?C temperature, don't!!! If your staining container tips over, you will ruin your cryostat!! Hopefully you are using high quality plus charge slides? We had frozen sections come off a plus charge slide after single 4?C acetone fixation on occasion. You can prevent frozen section loss is a Double Acetone fixation that also increases permeabilization. An IHC guru gave me this hint years ago and was given to her by a company selling immunostaining products. A small fan will be your best friend for RT air drying and/or evaporating acetone. However we air dried all FS were dried at RT for 30 minutes minimum or longer before fixation. By air drying, you get rid of the water. You can put your FS in front of a small fan, or inside a hood for faster drying, and never store just cut FS in the cryostat where water condensation occurs when you take them out of cryostat environment to RT. DRY frozen sections were the rule in our lab before any solvent fixation. 1. Air dry frozen section at RT for 30 min 2. Fix FS in 4?C acetone for 10 minutes 3. Air dry FS for 15 minutes to evaporate acetone 4. Return FS to 4?C acetone for 10 minutes 5. Air dry to evaporate acetone 6. Proceed to immunostaining Gayle Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Thu Mar 12 12:56:29 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Mar 12 12:56:34 2015 Subject: [Histonet] Embedding Question In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF367FC4D2@ex07.net.ucsf.edu> Message-ID: <46632d015fe5481eac04e1b74171c5e6@MAIL13M2N2.ad.uams.edu> We routinely pull tissue from the processor and in the time it takes to load the embedding centers in numeric and alpha case order the block cool and paraffin will harden. I would not call it freezing it has just cooled. We start embedding immediately and between the heat on the embedding center and the heat in the block storage area on the embedding center we are fine. On Saturday and/or Sunday we leave them in cooled paraffin until Monday morning, again no problem. We do not refrigerate it everything is room temperature and then put in embedding center for approximately 30 minutes before we start embedding. Sometimes for small biopsies we just set it on the staging area (10 blocks at a time) and start as soon as the person assigned has the station set up for their preference. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Thursday, March 12, 2015 12:08 PM To: Paula Sicurello Cc: HistoNet; Morken, Timothy Subject: Re: [Histonet] Embedding Question If I may, I'd like to piggy-back onto what Paula has mentioned regarding allowing paraffin infiltrated tissue to cool before embedding it. Hopefully someone can help both of us out, even if we seem to warm our infiltrated tissue differently (Paula's in a dry bin and mine in a wax bath). I work in a research lab where we work in large batches and time is not a priority like it is in a clinical setting. Rather than leaving 60-80 cassettes of infiltrated tissue soaking in a hot wax bath for hours at a time, we've begun to allow the cassettes to cool and just toss a handful of cassettes into the wax bath 5-10 minutes before we're ready to embed that batch of cassettes. Sometimes we don't even embed the cooled tissue until the next day or later that week. I haven't noticed an obvious difference in how our blocks section, though we have troublesome batches sometimes and we haven't been able to put our finger on why. Anyone know if allowing infiltrated tissue to cool and then reheat before embedding is better or worse than keeping the tissue soaking in wax for hours at a time? Thanks! Lucie Lucie Guernsey UC San Diego lguernsey@ucsd.edu On Thu, Mar 12, 2015 at 9:43 AM, Paula Sicurello wrote: > Hi Tim, > > There are several embedding events through-out the day, though mostly > in the wee hours of the morning. The embedding centers would be in > the same room as the microtomes (another question about those tomorrow). > > I worry about the small (GI, needles, etc) biopsies freezing before > they reach the embedding stations. In my experience, once they freeze > they get this outer wax coating (like a permeability barrier) which > doesn't melt when placed in the dry (no paraffin inside) but hot, holding bin. > > They just don't seem to embed that well and have a tendency to drop > out of the sections when cutting. > > Has anyone else had that happen? > > Paula > > On Thu, Mar 12, 2015 at 9:07 AM, Morken, Timothy > > wrote: > > > Paula, > > How many times per day? > > Is the embedding close to the cutting area? > > > > Of course any extra walking is a problem, especially in busy areas. > > Is this a non-patient area (hopefully!)? Any restructuring should be > > to move things closer together, not further away! > > > > Having said that, If it comes to that I would be more concerned > > about embedding proximity to the cutting area since having embedding > > near > cutting > > enhances workflow and cross coverage. If you don't unload processors > > very often then having them distant might not be too bad. Not ideal, > > but not a necessarily a deal killer. > > > > > > Tim Morken > > Pathology Site Manager, Parnassus > > Supervisor, Electron Microscopy/Neuromuscular Special Studies > > Department of Pathology UC San Francisco Medical Center > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula > > Sicurello > > Sent: Thursday, March 12, 2015 6:29 AM > > To: HistoNet > > Subject: [Histonet] Embedding Question > > > > It has been proposed to move the embedding centers to a room about > > 210 ft away from the tissue processors. > > > > The trip from processor to embedding center would take over 2 > > minutes and require the histotechs to carry the baskets full of > > cassettes down a much used hallway. > > > > Opinions? > > > > Do you feel this is a good idea-yes or no and why? > > > > Thanks in advance, > > > > Paula > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From suetp918 <@t> comcast.net Thu Mar 12 13:29:44 2015 From: suetp918 <@t> comcast.net (Sue) Date: Thu Mar 12 13:30:09 2015 Subject: [Histonet] Embedding Question In-Reply-To: References: <38667E7FB77ECD4E91BFAEB8D98638632616513FAF@LRGHEXVS1.practice.lrgh.org> <450B7A81EDA0C54E97C53D60F00776C3233738422B@isexstore03> Message-ID: <1507799002.210445.1426184984176.JavaMail.zimbra@comcast.net> I guess my first question would be why?? As far as the paraffin frezzing, we leave out biopsies out and when ready to embed we place them on the hot area of the embedding center until they are melted then embed and have no issues at all.?? The only thing I can think of is the area they are being held in and that temperature.? If you are looking for a lean approach this is not it.? If they are already in a space I would ask what the space is going to be used for and perhaps that use could be swiched. ? TJUH ? sue From suetp918 <@t> comcast.net Thu Mar 12 13:34:37 2015 From: suetp918 <@t> comcast.net (Sue) Date: Thu Mar 12 13:34:48 2015 Subject: [Histonet] Embedding Question In-Reply-To: <46632d015fe5481eac04e1b74171c5e6@MAIL13M2N2.ad.uams.edu> References: <761E2B5697F795489C8710BCC72141FF367FC4D2@ex07.net.ucsf.edu> <46632d015fe5481eac04e1b74171c5e6@MAIL13M2N2.ad.uams.edu> Message-ID: <1960789963.211334.1426185277910.JavaMail.zimbra@comcast.net> I actually have a bigger issue with moving the cassettes down the hall.? Patient Safety.? You may have the most cautious staff but what about everyone else.? all you need is one person to bump your tech and the blocks fall all over.? that is a QC nightmare.? When are you QCing your blocks before they leave the tissue processing room.? What is the possibility of one going lost and found days later.? I actually think this idea did not come from a lab person. ? TJUH Sue ? From aonomic <@t> auburn.edu Thu Mar 12 13:39:35 2015 From: aonomic <@t> auburn.edu (Michelle Aono) Date: Thu Mar 12 13:39:45 2015 Subject: [Histonet] Guinea Pig Inner Ear Message-ID: <3A862E73E3BAEF4ABCA665F7E3AF0640B2517DAB@exmb2> Hello Histonet! I am renewing my search for guinea pig inner ear slides to replace the old Turtox ones for our histo class. Does anyone know where I might acquire guinea pig inner ear slides? Or if I could get them as fixed, decalcified, embedded blocks, ready for sectioning? Or if you have guinea pig inner ears I could have... If you have ever sectioned inner ear I would love to hear from you! Orientation seems to be critical and the one attempt we did on dog IE was a miserable failure. Thanks for any and all help! Michelle (Shelly) Aono ~~~~~~~~~~~~~~~~~~~ Research Associate II 107B/124 Greene Hall Auburn University, Dept of APP Auburn, AL 36849 (334) 844-5594 From PAMarcum <@t> uams.edu Thu Mar 12 14:00:14 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Thu Mar 12 14:00:22 2015 Subject: [Histonet] Embedding Question In-Reply-To: <1960789963.211334.1426185277910.JavaMail.zimbra@comcast.net> References: <761E2B5697F795489C8710BCC72141FF367FC4D2@ex07.net.ucsf.edu> <46632d015fe5481eac04e1b74171c5e6@MAIL13M2N2.ad.uams.edu> <1960789963.211334.1426185277910.JavaMail.zimbra@comcast.net> Message-ID: <05cbe6452dbe41628d3ffb989f054eb8@MAIL13M2N2.ad.uams.edu> Sounds like CP Admin is getting involved and they want more space for something. We are back to assuming we can just get more tissue like they do blood and other samples. Pam From: Sue [mailto:suetp918@comcast.net] Sent: Thursday, March 12, 2015 1:35 PM To: Marcum, Pamela A Cc: Lucie Guernsey; Paula Sicurello; HistoNet; Timothy Morken Subject: Re: [Histonet] Embedding Question I actually have a bigger issue with moving the cassettes down the hall. Patient Safety. You may have the most cautious staff but what about everyone else. all you need is one person to bump your tech and the blocks fall all over. that is a QC nightmare. When are you QCing your blocks before they leave the tissue processing room. What is the possibility of one going lost and found days later. I actually think this idea did not come from a lab person. TJUH Sue ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Clough <@t> medicine.tamhsc.edu Thu Mar 12 14:06:53 2015 From: Clough <@t> medicine.tamhsc.edu (Clough, Bret) Date: Thu Mar 12 14:07:00 2015 Subject: [Histonet] What to charge for samples. Message-ID: Hello everyone, I would like to get some feedback on what you all would charge someone who sent you tissue to process, paraffin embedding, section ,and placing on slides(no staining). How much more would you charge to stain and coverslip using standard H&E? Your comment would be greatly appreciated. Thank you, Bret Clough Texas A&M Health Science Center Institute for Regenerative Medicine Temple, TX. From lblazek <@t> digestivespecialists.com Thu Mar 12 14:27:17 2015 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Mar 12 14:27:26 2015 Subject: [Histonet] job opening Message-ID: <5A2BD13465E061429D6455C8D6B40E39173567F95A@IBMB7Exchange.digestivespecialists.com> I'm posting this for Matt . And it is a great place to work! Linda Hey All We have a full time position now open at the Dayton Children's Hospital. This is a small lab, we process between 15 and 50 blocks per day. Pay will be determined according to experience. Must be certified or certified eligible with-in two years. Great place to work, little stress, great people and benefits. Follow the link to apply. http://www.childrensdayton.org/cms/sitelet/job_postings/index.html Click on Technical and Full Time Matt Chase, HT (ASCP) Supervisor of Pathology Dayton Children's One Children's Plaza Dayton, Ohio 45404-1815 Office Number: 937-641-3000 Ext 8229 Fax Number: 937-641-5482 Email: chasem@childrensdayton.org Website: childrensdayton.org From gayle.callis <@t> bresnan.net Thu Mar 12 16:00:22 2015 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Mar 12 16:00:30 2015 Subject: [Histonet] RE: Masson Trichrome stain Message-ID: <002501d05d07$8e7486e0$ab5d94a0$@bresnan.net> I have been following the string of inquiries about using metal forceps with Masson's Trichrome staining. I was taught many years ago to avoid metal forceps or the older metal tissue cassettes with Bouins. I am scrambling to find the actual reference. The reason given was acids in Bouins corrode metal. This may be a lost bit of information since the overall majority of labs now use plastic tissue cassettes. Case in point: using acidic descaling solutions for household cleaning i.e. showers/tubs or coffee machines. These solutions come with warning to avoid metal fixtures and stainless steel sinks. Accidental contact of acids in a stainless sink causes the metal discolor, indicating corrosion - been there, done that to a stainless steel sink. I so use metal forceps to move slides between Mass Tri staining solutions (and silver staining solutions) without problems per John Kiernan's comment. Not using metal forceps with silver stains i.e. GMS, reticulin, is to avoid metal ion contamination which is more likely due to with poorly washed glassware. In the past, we dipped metal forceps in melted paraffin, very messy since paraffin comes off on slides and in hot staining solutions. Disposable plastic forceps are cheap but break easily resulting in a dropped slide. Teflon forceps are pricey but it was a challenge to hold slides. Hopefully there are teflon forceps that work better than the one we used? We tried a teflon tipped metal forceps but not worth the price as teflon wears off the tips to rexpose metal. Weigerts hematoxylin is not affected by metal forceps since there are no acid components to corrode the metal although Weigerts can "stain" the forceps. Simply wash the forceps in dilute chlorine bleach then soap and water. I agree with John Kiernan and now use metal forceps to move slides between staining solutions in both Massons trichrome (and silver methods) without problems. If people want to use plastic or teflon forceps, I understand the reasons. As for not rinsing before going into Aniline Blue (or light green) in Massons trichrome, there is a reason for this. Sheehan and Hrapchak state verbatim " The phosphomolybdic acid and phosphotungstic acid thus acts as a link connecting basic groups of the connective tissue fiber to the basic groups of the dye i.e. aniline blue. The PM/PT acid treatment has the ultimate effect of making an amphoteric dye that would ordinarily act as an acid dye to change and act as a basic dye". These authors also say "Although the exact mechanism of how the stain works is unknown, some theories are available." By rinsing away the PT/PM, the link may be weaker hence one goes from PT/PM directly into aniline blue (sometimes light green or fast green). Bierbrich Scarlet/acid fuchsin and aniline blue (light green or fast green) solutions can be filtered back and reused many times. PT/PM and 1% acetic acid solutions should be discarded after use. Instead of kits due to expense and some kit deviations from classic Massons Trichrome method, I found I could buy excellent, reliable single staining solutions i.e. Biebrich Scarlet/Acid Fuchsin and Aniline Blue from Newcomer Supply or Poly Scientific to avoid exposure when weighing out carcinogenic dyes. Bouins is purchased from the vendor with the best price. However, PT/PM and acetic acid single use solutions were still made in house to save costs. I strongly recommend reading John Kiernan's " Methods for Connective Tissues" from his book , Histological and Histochemical Methods Theory and Practice for better explanation and understanding of Massons Trichrome chemistry. Collagen and muscle staining methods in Sheehan and Hrapchaks Theory and Practice of Histotechnology is not recent but a good start. Whew, a long reply but hope helps........................... Gayle Callis HTL/HT/MT(ASCP) Written is: Justine, I do not have any metal forceps in the special stains area, due to the reaction that they can cause when staining with silver. As a rule of thumb, it is just easier to use plastic all the way around. The Carson text does not state the use of only plastic forceps, but I would think that maybe they are concerned with a reaction between the Weigert's and the metal. That would be a stretch. As for no water before aniline blue, I believe the concentration is very weak and the water may dilute they dye even further. This would affect the staining results. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 ------------------------------ Message: 4 Date: Tue, 10 Mar 2015 00:31:56 -0500 From: John Kiernan <@t> uwo.ca> Subject: Re: [Histonet] FW: Masson's trichrome stain To: Linda Margraf <@t> gmail.com>, histonet <@t> lists.utsouthwestern.edu Cc: justinelanzon <@t> hotmail.com Message-ID: <7380eaed48941.54fe3b7c <@t> uwo.ca> Content-Type: text/plain; charset=iso-8859-1 The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? John Kiernan = = = On 08/03/15, Linda Margraf <@t> gmail.com> wrote: > Here is a message from Justine... > > From: Justine Lanzon [mailto:justinelanzon <@t> hotmail.com] > <@t> hotmail.com]> > Sent: Thursday, March 05, 2015 5:36 AM > To: lindamargraf <@t> gmail.com > Subject: Masson's trichrome stain > Hi, > I am doing a write up on Masson's trichrome stain however I cannot > answer these two questions: > - Why are plastic forceps used instead of metal ones to hold the > stained slide? > - Why do we not rinse before Alinine blue? > Can you please help me? > Many Thanks, > > Justine Lanzon From JRobinson <@t> pathology-associates.com Thu Mar 12 16:07:42 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Thu Mar 12 16:07:51 2015 Subject: [Histonet] Embedding Question In-Reply-To: References: <38667E7FB77ECD4E91BFAEB8D98638632616513FAF@LRGHEXVS1.practice.lrgh.org> <450B7A81EDA0C54E97C53D60F00776C3233738422B@isexstore03> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8D5F0@PAEXCH1.PathologyAssociates.local> This certainly does not sound ideal to be moving tissue blocks around the building. If this situation cannot be changed then perhaps a plastic bin with a snap on lid could help minimize the risk of losing blocks during transport. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Thursday, March 12, 2015 9:47 AM To: Hannen, Valerie Cc: HistoNet Subject: Re: [Histonet] Embedding Question Why the change? Space. There is a larger space available for histology. It would be optimum to either move the processors into the new space or leave the embedding centers where they are now. I mentioned did bring up about the samples/wax freezing in transit and the reply was "the experts didn't mention anything about that" The experts may not have been experienced histotechs. Paula On Thu, Mar 12, 2015 at 7:56 AM, Hannen, Valerie < Valerie.Hannen@parrishmed.com> wrote: > I do not think it to be a good idea. > > 1) What is the possibility of a "trip and fall" in transit? > Cassettes flying everywhere, possibility of losing any? > > 2) Dripping paraffin on a hallway floor-- a slip and fall scenario > for other people using the "much used" hallway. > > 3) As Tom stated.. not a lean system/process. > > Just my two cents! > > > Valerie Hannen,MLT(ASCP),HTL,SU (FL) > Section Chief, Histology > Parrish Medical Center > 951 N. Washington Ave. > Titusville,Florida 32796 > T: (321)268-6333 ext. 7506 > F: (321) 268-6149 > valerie.hannen@parrishmed.com > www.parrishmed.com > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, > Thomas > Sent: Thursday, March 12, 2015 10:32 AM > To: Paula Sicurello; HistoNet > Subject: RE: [Histonet] Embedding Question > > Not what I call a lean system. Why the change? > > Tom > > > Tom Podawiltz HT (ASCP) > AP Section Head > LRGHealthcare > 603-524-3211 ext: 3220 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula > Sicurello > Sent: Thursday, March 12, 2015 9:29 AM > To: HistoNet > Subject: [Histonet] Embedding Question > > It has been proposed to move the embedding centers to a room about 210 > ft away from the tissue processors. > > The trip from processor to embedding center would take over 2 minutes > and require the histotechs to carry the baskets full of cassettes down > a much used hallway. > > Opinions? > > Do you feel this is a good idea-yes or no and why? > > Thanks in advance, > > Paula > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and > confidential information intended only for the use of the recipient(s) > named above. If you are not the intended recipient, you may not > print,distribute, or copy this message or any attachments. If you > have received this communication in error, please notify the sender by > return e-mail and delete this message and any attachments from your > computer. Any views or opinions expressed are solely those of the > author and do not necessarily represent those of LRGHealthcare. > > ====================================== > "This email is intended solely for the use of the individual to whom > it is addressed and may contain information that is privileged, > confidential or otherwise exempt from disclosure under applicable law. > If the reader of this email is not the intended recipient or the > employee or agent responsible for delivering the message to the > intended recipient, you are hereby notified that any dissemination, > distribution, or copying of this communication is strictly prohibited. > If you have received this communication in error, please immediately > delete this message. Thank you" > ====================================== > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From gayle.callis <@t> bresnan.net Thu Mar 12 17:17:03 2015 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Mar 12 17:17:13 2015 Subject: [Histonet] Acetone fixing and tissue damage In-Reply-To: References: <000001d05ce6$4e899fa0$eb9cdee0$@bresnan.net> Message-ID: <003c01d05d12$4532b550$cf981ff0$@bresnan.net> Dear Bernice, This has always been my favorite solvent fixative for murine CD markers and some other markers/antigens but it can't be used for human CD4 and CD8 IHC. These human markers do not tolerate the alcohol component and won't stain. The late Dr. Chris van der Loos imparted this bit of information after I recommended he should try it. I think most people do human CD4 and CD8 on FFPE tissues now but other human markers might be similar to the CD4 and CD8 and not stain after acetone/absolute ethanol. Acetone/alcohol in this ratio has been by fixative of choice for the mouse and rat CD markers but I used it at RT, not cold. The alcohol should be absolute ethanol. With this in mind, a recommendation is do a fixation panel with different fixatives to optimize for any given antigen. Air drying the fresh tissue frozen section is a must though. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: Bernice Frederick [mailto:b-frederick@northwestern.edu] Sent: Thursday, March 12, 2015 11:49 AM To: gayle.callis@bresnan.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Acetone fixing and tissue damage We use a 3:1 ration of cold acetone/100% ETOH to fix frozens for IHC. Slides are always air dried first to remove any moisture. Usually 75 mls acetone,25 mls alcohol. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, March 12, 2015 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Acetone fixing and tissue damage You wrote: Hi Everyone. When I fix my cryosections in acetone, I am using HPLC grade 99.9% for 10 minutes at -20C. Would the Histology grade 99.5% be less damaging to them? Higher H20 content, i.e. less than 99.5% apparently is also very bad. With the HPLC grade I often get tissue damage, the tissue also floats off the slide causing a stringy effect. Fixing with 4% p-formaldehyde or 100% Methanol, prevented the antibody from recognizing the Nuclear Antigens. Looking for advice, Patrick. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 **************************************************************************** ********************* HPLC grade acetone is not necessary plus very expensive. Use ACS Certified Reagent 99.5% grade, not histology grade, which you can buy in gallon size. Maybe what you are calling histology grade is the ACS Certified Reagent grade but "Histology" grade implies a practical grade of acetone which is not a pure as the ACS certified Reagent grade. Also, 4?C acetone works just as well. If you are storing your acetone (in a staining jar) inside the cryostat to maintain a 20?C temperature, don't!!! If your staining container tips over, you will ruin your cryostat!! Hopefully you are using high quality plus charge slides? We had frozen sections come off a plus charge slide after single 4?C acetone fixation on occasion. You can prevent frozen section loss is a Double Acetone fixation that also increases permeabilization. An IHC guru gave me this hint years ago and was given to her by a company selling immunostaining products. A small fan will be your best friend for RT air drying and/or evaporating acetone. However we air dried all FS were dried at RT for 30 minutes minimum or longer before fixation. By air drying, you get rid of the water. You can put your FS in front of a small fan, or inside a hood for faster drying, and never store just cut FS in the cryostat where water condensation occurs when you take them out of cryostat environment to RT. DRY frozen sections were the rule in our lab before any solvent fixation. 1. Air dry frozen section at RT for 30 min 2. Fix FS in 4?C acetone for 10 minutes 3. Air dry FS for 15 minutes to evaporate acetone 4. Return FS to 4?C acetone for 10 minutes 5. Air dry to evaporate acetone 6. Proceed to immunostaining Gayle Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Thu Mar 12 17:23:31 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Thu Mar 12 17:23:36 2015 Subject: [Histonet] Embedding Question In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D8D5F0@PAEXCH1.PathologyAssociates.local> References: <38667E7FB77ECD4E91BFAEB8D98638632616513FAF@LRGHEXVS1.practice.lrgh.org> <450B7A81EDA0C54E97C53D60F00776C3233738422B@isexstore03> <204A03EB5A7F0A4BB1EEDD52A963829C16D8D5F0@PAEXCH1.PathologyAssociates.local> Message-ID: Thanks to all who submitted their input. Since the "experts" were going for lean in the new histology space, more than likely the embedding stations will go in there. It *is* more efficient to have it set up this way. I just worried about the itsy bitsy GI biopsies not embedding well. A majority of people who replied had experience with this type of lab layout and the tissue samples did not suffer any as a result of getting a tour of the hallway. Many had to travel much farther than 200 feet. As suggested by many, I will pass along the very good idea of using a sturdy bin with a nice fitting lid for transport. I will think of it this way: better to have the chance of dropping the basket and cassettes and getting wax on the floor, than to drop the basket and cassettes in NBF while traversing the hallway. Hazmat, here we come, if that were to spill. Happy Thursday! Paula On Thu, Mar 12, 2015 at 2:07 PM, Jeffrey Robinson < JRobinson@pathology-associates.com> wrote: > This certainly does not sound ideal to be moving tissue blocks around the > building. If this situation cannot be changed then perhaps a plastic bin > with a snap on lid could help minimize the risk of losing blocks during > transport. > > Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > Sent: Thursday, March 12, 2015 9:47 AM > To: Hannen, Valerie > Cc: HistoNet > Subject: Re: [Histonet] Embedding Question > > Why the change? Space. There is a larger space available for histology. > > It would be optimum to either move the processors into the new space or > leave the embedding centers where they are now. > > I mentioned did bring up about the samples/wax freezing in transit and the > reply was "the experts didn't mention anything about that" > > The experts may not have been experienced histotechs. > > Paula > > On Thu, Mar 12, 2015 at 7:56 AM, Hannen, Valerie < > Valerie.Hannen@parrishmed.com> wrote: > > > I do not think it to be a good idea. > > > > 1) What is the possibility of a "trip and fall" in transit? > > Cassettes flying everywhere, possibility of losing any? > > > > 2) Dripping paraffin on a hallway floor-- a slip and fall scenario > > for other people using the "much used" hallway. > > > > 3) As Tom stated.. not a lean system/process. > > > > Just my two cents! > > > > > > Valerie Hannen,MLT(ASCP),HTL,SU (FL) > > Section Chief, Histology > > Parrish Medical Center > > 951 N. Washington Ave. > > Titusville,Florida 32796 > > T: (321)268-6333 ext. 7506 > > F: (321) 268-6149 > > valerie.hannen@parrishmed.com > > www.parrishmed.com > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, > > Thomas > > Sent: Thursday, March 12, 2015 10:32 AM > > To: Paula Sicurello; HistoNet > > Subject: RE: [Histonet] Embedding Question > > > > Not what I call a lean system. Why the change? > > > > Tom > > > > > > Tom Podawiltz HT (ASCP) > > AP Section Head > > LRGHealthcare > > 603-524-3211 ext: 3220 > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula > > Sicurello > > Sent: Thursday, March 12, 2015 9:29 AM > > To: HistoNet > > Subject: [Histonet] Embedding Question > > > > It has been proposed to move the embedding centers to a room about 210 > > ft away from the tissue processors. > > > > The trip from processor to embedding center would take over 2 minutes > > and require the histotechs to carry the baskets full of cassettes down > > a much used hallway. > > > > Opinions? > > > > Do you feel this is a good idea-yes or no and why? > > > > Thanks in advance, > > > > Paula > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > THIS MESSAGE IS CONFIDENTIAL. > > This e-mail message and any attachments are proprietary and > > confidential information intended only for the use of the recipient(s) > > named above. If you are not the intended recipient, you may not > > print,distribute, or copy this message or any attachments. If you > > have received this communication in error, please notify the sender by > > return e-mail and delete this message and any attachments from your > > computer. Any views or opinions expressed are solely those of the > > author and do not necessarily represent those of LRGHealthcare. > > > > ====================================== > > "This email is intended solely for the use of the individual to whom > > it is addressed and may contain information that is privileged, > > confidential or otherwise exempt from disclosure under applicable law. > > If the reader of this email is not the intended recipient or the > > employee or agent responsible for delivering the message to the > > intended recipient, you are hereby notified that any dissemination, > > distribution, or copying of this communication is strictly prohibited. > > If you have received this communication in error, please immediately > > delete this message. Thank you" > > ====================================== > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This email and attachments may contain PHI that is privileged and > confidential and is not intended for any unauthorized person. If you, the > reader, are not the intended recipient you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. Do not read the email but instead reply to the sender and > destroy the message and any attachments. Thank you. > From JMacDonald <@t> mtsac.edu Thu Mar 12 17:41:30 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Mar 12 17:41:35 2015 Subject: [Histonet] Guinea Pig Inner Ear In-Reply-To: <3A862E73E3BAEF4ABCA665F7E3AF0640B2517DAB@exmb2> References: <3A862E73E3BAEF4ABCA665F7E3AF0640B2517DAB@exmb2> Message-ID: are you just looking at the cochlea? From: Michelle Aono To: "'histonet@lists.utsouthwestern.edu'" Date: 03/12/2015 11:40 AM Subject: [Histonet] Guinea Pig Inner Ear Sent by: histonet-bounces@lists.utsouthwestern.edu Hello Histonet! I am renewing my search for guinea pig inner ear slides to replace the old Turtox ones for our histo class. Does anyone know where I might acquire guinea pig inner ear slides? Or if I could get them as fixed, decalcified, embedded blocks, ready for sectioning? Or if you have guinea pig inner ears I could have... If you have ever sectioned inner ear I would love to hear from you! Orientation seems to be critical and the one attempt we did on dog IE was a miserable failure. Thanks for any and all help! Michelle (Shelly) Aono ~~~~~~~~~~~~~~~~~~~ Research Associate II 107B/124 Greene Hall Auburn University, Dept of APP Auburn, AL 36849 (334) 844-5594 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From choose4health <@t> yahoo.com Fri Mar 13 08:18:23 2015 From: choose4health <@t> yahoo.com (Nan Gray) Date: Fri Mar 13 08:21:15 2015 Subject: [Histonet] (no subject) Message-ID: <530909402.5735594.1426252703334.JavaMail.yahoo@mail.yahoo.com> please unsubscribe?Live with Love, Joy and Vibrant Health ? ? From KBRYAN1 <@t> PARTNERS.ORG Fri Mar 13 15:00:29 2015 From: KBRYAN1 <@t> PARTNERS.ORG (Bryan, Karen) Date: Fri Mar 13 15:00:35 2015 Subject: [Histonet] ATPase 9.4 Troubleshooting Message-ID: <32AA727CD9EE4F4E82781EB3C238B30851CC20BF@PHSX10MB16.partners.org> Dear Friends, I am having trouble with the ATPase 9.4 stain on muscle biopsies performed on frozen sections. The sections look extremely dark and almost have a digested look to them. To troubleshoot we: * made all new solutions, * opened a new container of ATPase (stored in the -15 deg. C. freezer) * the oven has been tested and confirmed to be stable at 40 deg. C. * the pH is stable at 9.4 (+/- 0.02) * only distilled water is used for all solutions where required and for rinses between steps. The ATPase 4.3 has some artifact but definitely is readable and not like the 9.4. All of the other sections for the other stains in the protocol (H&E, Trichrome, NADH, ORO, PAS) are coming out great and the sections do not show any freezing or microtomy artifacts. Has anyone else run into this problem? We have run the same muscle cases 3 times in the last 2 days after making the changes to all of the solutions to prepare the ATPase. What are we missing? I may be able to upload a photo if you need to see the issue clearly. Thank you so much for your help! Karen Karen P. Bryan, HT (ASCP)cm Sr. Histology Specialist Department of Pathology/Amory 3 Brigham & Women's Hospital 75 Francis Street/Boston 617-732-5454 (Internal extension 2-5454) The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From claycal44 <@t> yahoo.com Fri Mar 13 15:56:42 2015 From: claycal44 <@t> yahoo.com (nancy lowen) Date: Fri Mar 13 15:56:48 2015 Subject: [Histonet] RE: Masson Trichrome stain In-Reply-To: <002501d05d07$8e7486e0$ab5d94a0$@bresnan.net> References: <002501d05d07$8e7486e0$ab5d94a0$@bresnan.net> Message-ID: <364325812.4279170.1426280202976.JavaMail.yahoo@mail.yahoo.com> Just out of curiosity--what strength of Picric acid is in your Bouins fixative?Nancy On Thursday, March 12, 2015 2:02 PM, Gayle Callis wrote: I have been following the string of inquiries about using metal forceps with Masson's Trichrome staining.? I was taught many years ago to avoid metal forceps or the older metal tissue cassettes with Bouins.? I am scrambling to find the actual reference.? The reason given was acids in Bouins corrode metal.? This may be a lost bit of information since the overall majority of labs now use plastic tissue cassettes.? Case in point:? using acidic descaling solutions for household cleaning i.e. showers/tubs or coffee machines.? These solutions come with warning to avoid metal fixtures and stainless steel sinks.? Accidental contact of acids in a stainless sink causes the metal? discolor, indicating corrosion - been there, done that to a stainless steel sink.? I so use metal forceps to move slides between Mass Tri staining solutions (and silver staining solutions) without problems per John Kiernan's comment.? ? ? ? ? Not using metal forceps with silver stains i.e. GMS, reticulin, is to avoid metal ion contamination which is more likely due to with poorly washed glassware.? In the past, we dipped metal forceps in melted paraffin, very messy since paraffin comes off on slides and in hot staining solutions. Disposable plastic forceps are cheap but break easily resulting in a dropped slide.? Teflon forceps are pricey but it was a challenge to hold slides. Hopefully there are teflon forceps that work better than the one we used? We tried a teflon tipped metal forceps but not worth the price as teflon wears off the tips to rexpose metal.? ? Weigerts hematoxylin? is not affected by metal forceps since there are no acid components to corrode the metal although Weigerts can "stain" the forceps.? Simply wash the forceps in dilute chlorine bleach then soap and water.? I agree with John Kiernan and now use metal forceps to move slides between staining solutions in both Massons trichrome (and silver methods)? without problems.? If people want to use plastic or teflon forceps, I understand the reasons.? ? ? As for not rinsing before going into Aniline Blue (or light green) in Massons trichrome, there is a reason for this.? ? Sheehan and Hrapchak state verbatim " The phosphomolybdic acid and phosphotungstic acid thus acts as a link connecting basic groups of the connective tissue fiber to the basic groups of the dye i.e. aniline blue.? The PM/PT acid treatment has the ultimate effect of making an amphoteric dye that would ordinarily act as an acid dye to change and act as a basic dye".? These authors also say "Although the exact mechanism of how the stain works is unknown, some theories are available."? ? By rinsing away the PT/PM, the link may be weaker hence one goes from PT/PM directly into aniline blue (sometimes light green or fast green).? ? Bierbrich Scarlet/acid fuchsin and aniline blue (light green or fast green) solutions can be filtered back and reused many times.? PT/PM and 1%? acetic acid solutions? should be discarded after use. Instead of kits due to expense and some kit deviations from classic Massons Trichrome method, I found I could buy excellent, reliable single staining solutions i.e. Biebrich Scarlet/Acid Fuchsin and Aniline Blue from Newcomer Supply or Poly Scientific to avoid exposure when weighing out carcinogenic dyes.? Bouins is purchased from the vendor with the best price.? However, PT/PM and acetic acid single use solutions were still made in house to save costs.? ? ? I strongly recommend reading John Kiernan's? " Methods for Connective Tissues"? from his book , Histological and Histochemical Methods Theory and Practice? for better explanation and understanding of Massons Trichrome chemistry.? ? Collagen and muscle staining methods in Sheehan and Hrapchaks Theory and Practice of Histotechnology is not recent but a good start. Whew, a long reply but hope helps........................... Gayle Callis HTL/HT/MT(ASCP)? Written is:? Justine, I do not have any metal forceps in the special stains area, due to the reaction that they can cause when staining with silver.? As a rule of thumb, it is just easier to use plastic all the way around.? The Carson text does not state the use of only plastic forceps, but I would think that maybe they are concerned with a reaction between the Weigert's and the metal.? That would be a stretch. As for no water before aniline blue, I believe the concentration is very weak and the water may dilute they dye even further.? This would affect the staining results. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 ------------------------------ Message: 4 Date: Tue, 10 Mar 2015 00:31:56 -0500 From: John Kiernan <@t> uwo.ca> Subject: Re: [Histonet] FW: Masson's trichrome stain To: Linda Margraf <@t> gmail.com>, ? ? ? ? ? ? ? ? histonet <@t> lists.utsouthwestern.edu Cc: justinelanzon <@t> hotmail.com Message-ID: <7380eaed48941.54fe3b7c <@t> uwo.ca> Content-Type: text/plain; charset=iso-8859-1 The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? John Kiernan = = = On 08/03/15, Linda Margraf? <@t> gmail.com> wrote: > Here is a message from Justine... > > From: Justine Lanzon [mailto:justinelanzon <@t> hotmail.com] > <@t> hotmail.com]> > Sent: Thursday, March 05, 2015 5:36 AM > To: lindamargraf <@t> gmail.com > Subject: Masson's trichrome stain > Hi, > I am doing a write up on Masson's trichrome stain however I cannot > answer these two questions: > - Why are plastic forceps used instead of metal ones to hold the > stained slide? > - Why do we not rinse before Alinine blue? > Can you please help me? > Many Thanks, > > Justine Lanzon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Fri Mar 13 16:03:06 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Mar 13 16:03:16 2015 Subject: [Histonet] RE: Masson Trichrome stain In-Reply-To: <364325812.4279170.1426280202976.JavaMail.yahoo@mail.yahoo.com> References: <002501d05d07$8e7486e0$ab5d94a0$@bresnan.net> <364325812.4279170.1426280202976.JavaMail.yahoo@mail.yahoo.com> Message-ID: <2b5260939c7a440abf57ff2163b839fa@MAIL13M2N2.ad.uams.edu> I have always bought saturated Picric Acid and not a specific strength. Avoid making it from scratch!! Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nancy lowen Sent: Friday, March 13, 2015 3:57 PM To: gayle.callis@bresnan.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Masson Trichrome stain Just out of curiosity--what strength of Picric acid is in your Bouins fixative?Nancy On Thursday, March 12, 2015 2:02 PM, Gayle Callis wrote: I have been following the string of inquiries about using metal forceps with Masson's Trichrome staining.? I was taught many years ago to avoid metal forceps or the older metal tissue cassettes with Bouins.? I am scrambling to find the actual reference.? The reason given was acids in Bouins corrode metal.? This may be a lost bit of information since the overall majority of labs now use plastic tissue cassettes.? Case in point:? using acidic descaling solutions for household cleaning i.e. showers/tubs or coffee machines.? These solutions come with warning to avoid metal fixtures and stainless steel sinks.? Accidental contact of acids in a stainless sink causes the metal? discolor, indicating corrosion - been there, done that to a stainless steel sink.? I so use metal forceps to move slides between Mass Tri staining solutions (and silver staining solutions) without problems per John Kiernan's comment.? ? ? ? ? Not using metal forceps with silver stains i.e. GMS, reticulin, is to avoid metal ion contamination which is more likely due to with poorly washed glassware.? In the past, we dipped metal forceps in melted paraffin, very messy since paraffin comes off on slides and in hot staining solutions. Disposable plastic forceps are cheap but break easily resulting in a dropped slide.? Teflon forceps are pricey but it was a challenge to hold slides. Hopefully there are teflon forceps that work better than the one we used? We tried a teflon tipped metal forceps but not worth the price as teflon wears off the tips to rexpose metal.? ? Weigerts hematoxylin? is not affected by metal forceps since there are no acid components to corrode the metal although Weigerts can "stain" the forceps.? Simply wash the forceps in dilute chlorine bleach then soap and water.? I agree with John Kiernan and now use metal forceps to move slides between staining solutions in both Massons trichrome (and silver methods)? without problems.? If people want to use plastic or teflon forceps, I understand the reasons.? ? ? As for not rinsing before going into Aniline Blue (or light green) in Massons trichrome, there is a reason for this.? ? Sheehan and Hrapchak state verbatim " The phosphomolybdic acid and phosphotungstic acid thus acts as a link connecting basic groups of the connective tissue fiber to the basic groups of the dye i.e. aniline blue.? The PM/PT acid treatment has the ultimate effect of making an amphoteric dye that would ordinarily act as an acid dye to change and act as a basic dye".? These authors also say "Although the exact mechanism of how the stain works is unknown, some theories are available."? ? By rinsing away the PT/PM, the link may be weaker hence one goes from PT/PM directly into aniline blue (sometimes light green or fast green).? ? Bierbrich Scarlet/acid fuchsin and aniline blue (light green or fast green) solutions can be filtered back and reused many times.? PT/PM and 1%? acetic acid solutions? should be discarded after use. Instead of kits due to expense and some kit deviations from classic Massons Trichrome method, I found I could buy excellent, reliable single staining solutions i.e. Biebrich Scarlet/Acid Fuchsin and Aniline Blue from Newcomer Supply or Poly Scientific to avoid exposure when weighing out carcinogenic dyes.? Bouins is purchased from the vendor with the best price.? However, PT/PM and acetic acid single use solutions were still made in house to save costs.? ? ? I strongly recommend reading John Kiernan's? " Methods for Connective Tissues"? from his book , Histological and Histochemical Methods Theory and Practice? for better explanation and understanding of Massons Trichrome chemistry.? ? Collagen and muscle staining methods in Sheehan and Hrapchaks Theory and Practice of Histotechnology is not recent but a good start. Whew, a long reply but hope helps........................... Gayle Callis HTL/HT/MT(ASCP)? Written is:? Justine, I do not have any metal forceps in the special stains area, due to the reaction that they can cause when staining with silver.? As a rule of thumb, it is just easier to use plastic all the way around.? The Carson text does not state the use of only plastic forceps, but I would think that maybe they are concerned with a reaction between the Weigert's and the metal.? That would be a stretch. As for no water before aniline blue, I believe the concentration is very weak and the water may dilute they dye even further.? This would affect the staining results. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 ------------------------------ Message: 4 Date: Tue, 10 Mar 2015 00:31:56 -0500 From: John Kiernan <@t> uwo.ca> Subject: Re: [Histonet] FW: Masson's trichrome stain To: Linda Margraf <@t> gmail.com>, ? ? ? ? ? ? ? ? histonet <@t> lists.utsouthwestern.edu Cc: justinelanzon <@t> hotmail.com Message-ID: <7380eaed48941.54fe3b7c <@t> uwo.ca> Content-Type: text/plain; charset=iso-8859-1 The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? John Kiernan = = = On 08/03/15, Linda Margraf? <@t> gmail.com> wrote: > Here is a message from Justine... > > From: Justine Lanzon [mailto:justinelanzon <@t> hotmail.com] > <@t> hotmail.com]> > Sent: Thursday, March 05, 2015 5:36 AM > To: lindamargraf <@t> gmail.com > Subject: Masson's trichrome stain > Hi, > I am doing a write up on Masson's trichrome stain however I cannot > answer these two questions: > - Why are plastic forceps used instead of metal ones to hold the > stained slide? > - Why do we not rinse before Alinine blue? > Can you please help me? > Many Thanks, > > Justine Lanzon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From juliep2341 <@t> gmail.com Fri Mar 13 18:03:11 2015 From: juliep2341 <@t> gmail.com (Julie Powell) Date: Fri Mar 13 18:03:11 2015 Subject: [Histonet] Medite Equipment Message-ID: <002101d05de1$e16c4ba0$a444e2e0$@com> RE: Medite Equipment I was wondering if anyone has any experience with the Medite ACS720 Coverslipper or TST44 Stainer? We are thinking of purchasing one and would like to get opinions on the quality. Thank you Julie From gu.lang <@t> gmx.at Sat Mar 14 03:14:29 2015 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Mar 14 03:14:38 2015 Subject: AW: [Histonet] RE: Masson Trichrome stain In-Reply-To: <364325812.4279170.1426280202976.JavaMail.yahoo@mail.yahoo.com> References: <002501d05d07$8e7486e0$ab5d94a0$@bresnan.net> <364325812.4279170.1426280202976.JavaMail.yahoo@mail.yahoo.com> Message-ID: <000f01d05e2e$e747e6e0$b5d7b4a0$@gmx.at> Hi Nancy, saturation of picric acid should be 14 g/l at 20?C and 60 g/l at 100?C (wikipedia). Wiki also states 0,04 M for picric acid in Bouins. 229 g/mol --> 9 g picric acid in 1 l Bouin. --> ca. 0,9% (Their Bouin contains 85% saturated picric acid) recept: 60 ml picric acid, 20 ml formaldehyde, 4 ml acetic acid Bouin is made of ca. 70% saturated picric acid. My rough estimation is 9,8 g/l picric acid in Bouin. Over the thumb 0,9 % in Bouin. Romeis states saturated picric acid is 1,2%. That would also bring a 0,9% concentration of picric acid in Bouin. Another question comes to my mind: We distinguish between hot saturated and cold saturated solution. Is the hot saturated solution after cooling able to hold more picric acid than the cold? If one starts with 60 g/l for saturated picric acid. This leads to about 3,6%. I hope mathematics and chemists won't beat me for my calculation. ;-) Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von nancy lowen Gesendet: Freitag, 13. M?rz 2015 21:57 An: gayle.callis@bresnan.net; histonet@lists.utsouthwestern.edu Betreff: Re: [Histonet] RE: Masson Trichrome stain Just out of curiosity--what strength of Picric acid is in your Bouins fixative?Nancy On Thursday, March 12, 2015 2:02 PM, Gayle Callis wrote: I have been following the string of inquiries about using metal forceps with Masson's Trichrome staining. I was taught many years ago to avoid metal forceps or the older metal tissue cassettes with Bouins. I am scrambling to find the actual reference. The reason given was acids in Bouins corrode metal. This may be a lost bit of information since the overall majority of labs now use plastic tissue cassettes. Case in point: using acidic descaling solutions for household cleaning i.e. showers/tubs or coffee machines. These solutions come with warning to avoid metal fixtures and stainless steel sinks. Accidental contact of acids in a stainless sink causes the metal discolor, indicating corrosion - been there, done that to a stainless steel sink. I so use metal forceps to move slides between Mass Tri staining solutions (and silver staining solutions) without problems per John Kiernan's comment. Not using metal forceps with silver stains i.e. GMS, reticulin, is to avoid metal ion contamination which is more likely due to with poorly washed glassware. In the past, we dipped metal forceps in melted paraffin, very messy since paraffin comes off on slides and in hot staining solutions. Disposable plastic forceps are cheap but break easily resulting in a dropped slide. Teflon forceps are pricey but it was a challenge to hold slides. Hopefully there are teflon forceps that work better than the one we used? We tried a teflon tipped metal forceps but not worth the price as teflon wears off the tips to rexpose metal. Weigerts hematoxylin is not affected by metal forceps since there are no acid components to corrode the metal although Weigerts can "stain" the forceps. Simply wash the forceps in dilute chlorine bleach then soap and water. I agree with John Kiernan and now use metal forceps to move slides between staining solutions in both Massons trichrome (and silver methods) without problems. If people want to use plastic or teflon forceps, I understand the reasons. As for not rinsing before going into Aniline Blue (or light green) in Massons trichrome, there is a reason for this. Sheehan and Hrapchak state verbatim " The phosphomolybdic acid and phosphotungstic acid thus acts as a link connecting basic groups of the connective tissue fiber to the basic groups of the dye i.e. aniline blue. The PM/PT acid treatment has the ultimate effect of making an amphoteric dye that would ordinarily act as an acid dye to change and act as a basic dye". These authors also say "Although the exact mechanism of how the stain works is unknown, some theories are available." By rinsing away the PT/PM, the link may be weaker hence one goes from PT/PM directly into aniline blue (sometimes light green or fast green). Bierbrich Scarlet/acid fuchsin and aniline blue (light green or fast green) solutions can be filtered back and reused many times. PT/PM and 1% acetic acid solutions should be discarded after use. Instead of kits due to expense and some kit deviations from classic Massons Trichrome method, I found I could buy excellent, reliable single staining solutions i.e. Biebrich Scarlet/Acid Fuchsin and Aniline Blue from Newcomer Supply or Poly Scientific to avoid exposure when weighing out carcinogenic dyes. Bouins is purchased from the vendor with the best price. However, PT/PM and acetic acid single use solutions were still made in house to save costs. I strongly recommend reading John Kiernan's " Methods for Connective Tissues" from his book , Histological and Histochemical Methods Theory and Practice for better explanation and understanding of Massons Trichrome chemistry. Collagen and muscle staining methods in Sheehan and Hrapchaks Theory and Practice of Histotechnology is not recent but a good start. Whew, a long reply but hope helps........................... Gayle Callis HTL/HT/MT(ASCP) Written is: Justine, I do not have any metal forceps in the special stains area, due to the reaction that they can cause when staining with silver. As a rule of thumb, it is just easier to use plastic all the way around. The Carson text does not state the use of only plastic forceps, but I would think that maybe they are concerned with a reaction between the Weigert's and the metal. That would be a stretch. As for no water before aniline blue, I believe the concentration is very weak and the water may dilute they dye even further. This would affect the staining results. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 ------------------------------ Message: 4 Date: Tue, 10 Mar 2015 00:31:56 -0500 From: John Kiernan <@t> uwo.ca> Subject: Re: [Histonet] FW: Masson's trichrome stain To: Linda Margraf <@t> gmail.com>, histonet <@t> lists.utsouthwestern.edu Cc: justinelanzon <@t> hotmail.com Message-ID: <7380eaed48941.54fe3b7c <@t> uwo.ca> Content-Type: text/plain; charset=iso-8859-1 The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? John Kiernan = = = On 08/03/15, Linda Margraf <@t> gmail.com> wrote: > Here is a message from Justine... > > From: Justine Lanzon [mailto:justinelanzon <@t> hotmail.com] > <@t> hotmail.com]> > Sent: Thursday, March 05, 2015 5:36 AM > To: lindamargraf <@t> gmail.com > Subject: Masson's trichrome stain > Hi, > I am doing a write up on Masson's trichrome stain however I cannot > answer these two questions: > - Why are plastic forceps used instead of metal ones to hold the > stained slide? > - Why do we not rinse before Alinine blue? > Can you please help me? > Many Thanks, > > Justine Lanzon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shariros <@t> msn.com Sun Mar 15 11:14:29 2015 From: shariros <@t> msn.com (Shari Rosen) Date: Sun Mar 15 11:14:35 2015 Subject: [Histonet] Mailing list Message-ID: Sent from my iPhone From tony.auge <@t> gmail.com Sun Mar 15 14:57:42 2015 From: tony.auge <@t> gmail.com (Tony Auge) Date: Sun Mar 15 14:57:46 2015 Subject: [Histonet] Molecular Biology, MB(ASCP) study materials. Message-ID: Hi Histonetters, I am interested in taking the the Molecular Biologist certification through ASCP. Can anyone recommend some pertinent studying materials for this exam please? Thanks, Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.auge@gmail.com From Jonathan.Arzt <@t> ARS.USDA.GOV Mon Mar 16 07:27:38 2015 From: Jonathan.Arzt <@t> ARS.USDA.GOV (Arzt, Jonathan) Date: Mon Mar 16 07:28:15 2015 Subject: [Histonet] Federal HT Job Opportunity at Plum Island Message-ID: <7213AC15544DE945843D821E1769E38A06A26FAB@001FSN2MPN1-052.001f.mgd2.msft.net> Immediately available and open for application for just 4 more days, is a full-time, permanent, federal histotchnologist position with the Foreign Animal Disease Research Unit at the Plum Island Animal Disease Center. Job Title: Biologist (Histotechnologist) Department: Department Of Agriculture Agency: Agricultural Research Service Job Announcement Number: ARS-S15E-0045 SALARY RANGE: $44,617.00 to $85,841.00 / Per Year See the full posting and application details here: https://www.usajobs.gov/GetJob/ViewDetails/396084800 From jqb7 <@t> cdc.gov Mon Mar 16 12:20:52 2015 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Mon Mar 16 12:21:09 2015 Subject: [Histonet] Histology position at CDC Atlanta Message-ID: <3B2CD438E1628A41BD687E98B963B78137F0674B@EMBX-CLFT4.cdc.gov> We are currently accepting resum?s for a Histotechnician, HT(ASCP) or Histotechnologist., HTL(ASCP) at the Centers for Disease Control and Prevention in Atlanta, GA. The ideal candidate will have a minimum of 2 years' experience in the histology lab to include processing, embedding, sectioning and both routine and special staining of slides. We have state-of-the-art instrumentation and are currently looking into a barcoding system. This is not an entry level position and is currently being offered as a contract position. If interested, please contact me with your resum? and I will be happy to share more details. Jeanine H. Sanders, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 jqb7@cdc.gov From lcjones7 <@t> alaska.edu Mon Mar 16 12:23:05 2015 From: lcjones7 <@t> alaska.edu (Leesa Jones) Date: Mon Mar 16 12:23:10 2015 Subject: [Histonet] Refurbished Equipment Message-ID: I am looking at purchasing a refurbished processor from either RBC, GMI or IMEB. I would greatly appreciate any feedback/experiences other Histonetter's have had with these companies. If there is another company that you purchased refurbished equipment from and had a wonderful experience with and would like to recommend them, I would love to know. PM me at lcjones7@alaska.edu Thank you!!! -- *Leesa C. Jones* Animal Resources Center - Veterinary Services University of Alaska, Fairbanks 907-474-6054 or 907-474-7020 lcjones7@alaska.edu From tina.vanmeter <@t> gmail.com Mon Mar 16 12:53:47 2015 From: tina.vanmeter <@t> gmail.com (Tina Van Meter) Date: Mon Mar 16 12:53:51 2015 Subject: [Histonet] Refurbished Equipment In-Reply-To: References: Message-ID: Hi Leesa, I have dealt with IMEB and have nothing but great things to say concerning their equipment and customer service. The equipment was in top notch condition and they contact me periodically to make sure everything is working well. Good luck, Tina T?ina Van Meter, HT (ASCP) Histology Core Manager Scripps Research Institute Jupiter, Florida On Mon, Mar 16, 2015 at 1:23 PM, Leesa Jones wrote: > I am looking at purchasing a refurbished processor from either RBC, GMI or > IMEB. I would greatly appreciate any feedback/experiences other > Histonetter's have had with these companies. If there is another company > that you purchased refurbished equipment from and had a wonderful > experience with and would like to recommend them, I would love to know. PM > me at lcjones7@alaska.edu > > Thank you!!! > -- > *Leesa C. Jones* > > Animal Resources Center - Veterinary Services > University of Alaska, Fairbanks > 907-474-6054 or 907-474-7020 > lcjones7@alaska.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From TanyaAbbott <@t> catholichealth.net Mon Mar 16 14:33:11 2015 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Mon Mar 16 14:34:11 2015 Subject: [Histonet] Release of blocks to research facilities Message-ID: <852F7D2C14FB464D80E182B15DB138AF4CDC9E6A@CHIEX005.CHI.catholichealth.net> CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. Thanks in advance for your help! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From mjones <@t> metropath.com Mon Mar 16 14:41:19 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Mon Mar 16 14:41:25 2015 Subject: [Histonet] Release of blocks to research facilities Message-ID: Following this closely. . . Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 3/16/15, 1:33 PM, "Abbott, Tanya" wrote: >CAP checklist ANP.12500 refers to "Record Retention". I am looking >specifically at NOTE 2: "Regarding extra-institutional release of blocks >for research purposes." >I am wondering how everyone handles this, especially if you have only 1 >block on newly diagnosed patient and the Doctor wants it sent out for >research. >Thanks in advance for your help! Tanya > >Tanya G. Abbott >Manager Technologist >Histology/Cytology >St Joseph Medical Center >(phone) 610-378-2635 > >This email and attachments contain information that may be confidential >or privileged. If you are not the intended recipient, notify the sender >at once and delete this message completely from your information system. >Further use, disclosure, or copying of information contained in this >email is not authorized, and any such action should not be construed as a >waiver of privilege or other confidentiality protections. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From blayjorge <@t> gmail.com Tue Mar 17 05:53:37 2015 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Tue Mar 17 05:53:42 2015 Subject: [Histonet] Comet assay: looking for reviewers of a short paper Message-ID: Dear Histonetters: I serve as editor-in-chief of the peer-reviewed scientific journal, *Life: The Excitement of Biology*. I just received a short (ca. 4,400 words long, incl. Literature Cited and Figure Legends) paper that assesses DNA damage levels in a species of mussel using the comet assay. If you are interested in serving as a reviewer, please send me an email directly (= off the list) to blayjorge@gmail.com. Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From TanyaAbbott <@t> catholichealth.net Tue Mar 17 07:38:00 2015 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Tue Mar 17 07:38:09 2015 Subject: [Histonet] FW: Release of blocks to research facilities Message-ID: <852F7D2C14FB464D80E182B15DB138AF4CDCA504@CHIEX005.CHI.catholichealth.net> I did call the CAP and they stated first of all, that it is a very confusing checklist component! But I was told that the research facility is taking on the ownership of the block, and we need to ensure that whoever we are sending our blocks to are following the proper retention protocols. From: Abbott, Tanya Sent: Monday, March 16, 2015 3:33 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Release of blocks to research facilities CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. Thanks in advance for your help! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From DKBoyd <@t> chs.net Tue Mar 17 08:11:51 2015 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Tue Mar 17 08:12:30 2015 Subject: [Histonet] RE: Release of blocks to research facilities In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF4CDC9E6A@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF4CDC9E6A@CHIEX005.CHI.catholichealth.net> Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9F0FF35@TN001WEXMBX014.US.chs.net> I send them without hesitation. Ours is to assist the patient not get tangled up in regulatory bureaucracy. Just as you wouldn't hesitate to send a subpoenaed block. The point here is to KNOW where your blocks are when not on site. Yes we are custodians of blocks and slides, but you mustn't impede patient care. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Abbott, Tanya [TanyaAbbott@catholichealth.net] Sent: Monday, March 16, 2015 3:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Release of blocks to research facilities CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. Thanks in advance for your help! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Fawn.Bomar <@t> HalifaxRegional.com Tue Mar 17 08:47:55 2015 From: Fawn.Bomar <@t> HalifaxRegional.com (Fawn Bomar) Date: Tue Mar 17 08:48:32 2015 Subject: [Histonet] ER/PR Benchmarks Message-ID: <0111BC10D77DC54EAB99B2DDA3BCE4B9832342@EXCH-2K10.hrhs.com> Hi all, &n Does anyone know where to find the ER/PR benchmark numbers are to use to assess our annual numbers? Thank you Fawn ----------------------------------- This electronic message may contain inf confidential or legally privileged. It is inten for the use of the individual(s) and entity named as reci pients in the message. If you are not an inten notify the sender immediate computer. Do not deliver, dis and do not disclose its contents o the information it contains. Thank you From wdesalvo.cac <@t> outlook.com Tue Mar 17 10:45:02 2015 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Tue Mar 17 10:45:15 2015 Subject: [Histonet] FW: Release of blocks to research facilities In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF4CDCA504@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF4CDCA504@CHIEX005.CHI.catholichealth.net> Message-ID: There are other issues, besides CAP, that you should consider when releasing blocks. 1. A block is a medical record. Has the patient consented to release for research? 2. If the block is released prior to 14 days discharge and there are any charges, the charge must be sent to your hospital. Post 14 days the charges are sent to insurance and patient. 3. Will the research lead to a commercial product? Again, written patient consent. 4. You may be able to charge the research facility for sending the block. Administrative costs. 5. Communicate with patient is a must. 6. Get written consent and have the receiving facility verify they will handle properly, retain or return the block d you will get copies of data for patient file. Make sure you reduce your risk. Sent from my iPhone > On Mar 17, 2015, at 5:38 AM, Abbott, Tanya wrote: > > I did call the CAP and they stated first of all, that it is a very confusing checklist component! But I was told that the research facility is taking on the ownership of the block, and we need to ensure that whoever we are sending our blocks to are following the proper retention protocols. > > From: Abbott, Tanya > Sent: Monday, March 16, 2015 3:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Release of blocks to research facilities > > CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." > I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. > Thanks in advance for your help! Tanya > > Tanya G. Abbott > Manager Technologist > Histology/Cytology > St Joseph Medical Center > (phone) 610-378-2635 > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson <@t> gnf.org Tue Mar 17 11:18:26 2015 From: JWatson <@t> gnf.org (James Watson) Date: Tue Mar 17 11:18:31 2015 Subject: [Histonet] FW: Release of blocks to research facilities In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF4CDCA504@CHIEX005.CHI.catholichealth.net> Message-ID: Bill is correct, CAP is actually the least of your worries. There are many regulations about human tissue used in research (I do not know them all), but patient consent is essential and in some cases the patient must actually agree to the specific research project. Here we have regulations about control block tissue that we use, we cannot get tissue directly from a local hospital. Also the laws are different in each country. There is a lot of bioethics involved. http://bioethics.od.nih.gov/humantissue.html Human Tissue Ownership and Use in Research: What Laboratorians and Researchers Should Know http://www.clinchem.org/content/56/11/1675.full.pdf James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Tuesday, March 17, 2015 8:45 AM To: Abbott, Tanya Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] FW: Release of blocks to research facilities There are other issues, besides CAP, that you should consider when releasing blocks. 1. A block is a medical record. Has the patient consented to release for research? 2. If the block is released prior to 14 days discharge and there are any charges, the charge must be sent to your hospital. Post 14 days the charges are sent to insurance and patient. 3. Will the research lead to a commercial product? Again, written patient consent. 4. You may be able to charge the research facility for sending the block. Administrative costs. 5. Communicate with patient is a must. 6. Get written consent and have the receiving facility verify they will handle properly, retain or return the block d you will get copies of data for patient file. Make sure you reduce your risk. Sent from my iPhone > On Mar 17, 2015, at 5:38 AM, Abbott, Tanya wrote: > > I did call the CAP and they stated first of all, that it is a very confusing checklist component! But I was told that the research facility is taking on the ownership of the block, and we need to ensure that whoever we are sending our blocks to are following the proper retention protocols. > > From: Abbott, Tanya > Sent: Monday, March 16, 2015 3:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Release of blocks to research facilities > > CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." > I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. > Thanks in advance for your help! Tanya > > Tanya G. Abbott > Manager Technologist > Histology/Cytology > St Joseph Medical Center > (phone) 610-378-2635 > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robrankin <@t> rankinbiomed.com Tue Mar 17 12:27:34 2015 From: robrankin <@t> rankinbiomed.com (Rob Rankin) Date: Tue Mar 17 12:27:45 2015 Subject: [Histonet] Lead ( #3 ) In-Reply-To: <55085dc4.061cb60a.4f3a.ffff9d8dSMTPIN_ADDED_MISSING@mx.google.com> References: <55085dc4.061cb60a.4f3a.ffff9d8dSMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Here is a lead ( #3 ), but IMEB has a leg up with an endorsement already. Please ask Dawn Truscott or someone else to post a Rankin endorsement on Histonet ASAP and contact the prospect, Sent from my iPhone > On Mar 17, 2015, at 7:00 AM, histonet-request@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Histology position at CDC Atlanta > (Sanders, Jeanine (CDC/OID/NCEZID)) > 2. Refurbished Equipment (Leesa Jones) > 3. Re: Refurbished Equipment (Tina Van Meter) > 4. Release of blocks to research facilities (Abbott, Tanya) > 5. Re: Release of blocks to research facilities (Michael Ann Jones) > 6. Comet assay: looking for reviewers of a short paper > (Jorge A. Santiago-Blay) > 7. FW: Release of blocks to research facilities (Abbott, Tanya) > 8. RE: Release of blocks to research facilities (Boyd, Debbie M) > 9. ER/PR Benchmarks (Fawn Bomar) > 10. Re: FW: Release of blocks to research facilities (WILLIAM DESALVO) > 11. RE: FW: Release of blocks to research facilities (James Watson) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 16 Mar 2015 17:20:52 +0000 > From: "Sanders, Jeanine (CDC/OID/NCEZID)" > Subject: [Histonet] Histology position at CDC Atlanta > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <3B2CD438E1628A41BD687E98B963B78137F0674B@EMBX-CLFT4.cdc.gov> > Content-Type: text/plain; charset="iso-8859-1" > > We are currently accepting resum?s for a Histotechnician, HT(ASCP) or Histotechnologist., HTL(ASCP) at the Centers for Disease Control and Prevention in Atlanta, GA. > > The ideal candidate will have a minimum of 2 years' experience in the histology lab to include processing, embedding, sectioning and both routine and special staining of slides. > > We have state-of-the-art instrumentation and are currently looking into a barcoding system. > > This is not an entry level position and is currently being offered as a contract position. > > If interested, please contact me with your resum? and I will be happy to share more details. > > > Jeanine H. Sanders, BS HT(ASCP), QIHC > Centers for Disease Control and Prevention > Infectious Diseases Pathology Branch > 1600 Clifton Road, NE > MS/G-32 > Atlanta, Ga 30333 > 404-639-3590 > jqb7@cdc.gov > > > > > ------------------------------ > > Message: 2 > Date: Mon, 16 Mar 2015 09:23:05 -0800 > From: Leesa Jones > Subject: [Histonet] Refurbished Equipment > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > I am looking at purchasing a refurbished processor from either RBC, GMI or > IMEB. I would greatly appreciate any feedback/experiences other > Histonetter's have had with these companies. If there is another company > that you purchased refurbished equipment from and had a wonderful > experience with and would like to recommend them, I would love to know. PM > me at lcjones7@alaska.edu > > Thank you!!! > -- > *Leesa C. Jones* > > Animal Resources Center - Veterinary Services > University of Alaska, Fairbanks > 907-474-6054 or 907-474-7020 > lcjones7@alaska.edu > > > ------------------------------ > > Message: 3 > Date: Mon, 16 Mar 2015 13:53:47 -0400 > From: Tina Van Meter > Subject: Re: [Histonet] Refurbished Equipment > To: Leesa Jones > Cc: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Hi Leesa, > I have dealt with IMEB and have nothing but great things to say concerning > their equipment and customer service. The equipment was in top notch > condition and they contact me periodically to make sure everything is > working well. > > Good luck, > Tina > > T???ina Van Meter, HT (ASCP) > Histology Core Manager > Scripps Research Institute > Jupiter, Florida > >> On Mon, Mar 16, 2015 at 1:23 PM, Leesa Jones wrote: >> >> I am looking at purchasing a refurbished processor from either RBC, GMI or >> IMEB. I would greatly appreciate any feedback/experiences other >> Histonetter's have had with these companies. If there is another company >> that you purchased refurbished equipment from and had a wonderful >> experience with and would like to recommend them, I would love to know. PM >> me at lcjones7@alaska.edu >> >> Thank you!!! >> -- >> *Leesa C. Jones* >> >> Animal Resources Center - Veterinary Services >> University of Alaska, Fairbanks >> 907-474-6054 or 907-474-7020 >> lcjones7@alaska.edu >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 4 > Date: Mon, 16 Mar 2015 19:33:11 +0000 > From: "Abbott, Tanya" > Subject: [Histonet] Release of blocks to research facilities > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <852F7D2C14FB464D80E182B15DB138AF4CDC9E6A@CHIEX005.CHI.catholichealth.net> > > Content-Type: text/plain; charset="us-ascii" > > CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." > I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. > Thanks in advance for your help! Tanya > > Tanya G. Abbott > Manager Technologist > Histology/Cytology > St Joseph Medical Center > (phone) 610-378-2635 > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > > > ------------------------------ > > Message: 5 > Date: Mon, 16 Mar 2015 19:41:19 +0000 > From: Michael Ann Jones > Subject: Re: [Histonet] Release of blocks to research facilities > To: "Abbott, Tanya" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Following this closely. . . > Michael Ann > Michael Ann Jones, HT (ASCP) > Histology Manager > Metropath > 7444 W. Alaska Dr. #250 > Lakewood, CO 80226 > 303.634.2511 > Mjones@metropath.com > > > > > > > On 3/16/15, 1:33 PM, "Abbott, Tanya" > wrote: > >> CAP checklist ANP.12500 refers to "Record Retention". I am looking >> specifically at NOTE 2: "Regarding extra-institutional release of blocks >> for research purposes." >> I am wondering how everyone handles this, especially if you have only 1 >> block on newly diagnosed patient and the Doctor wants it sent out for >> research. >> Thanks in advance for your help! Tanya >> >> Tanya G. Abbott >> Manager Technologist >> Histology/Cytology >> St Joseph Medical Center >> (phone) 610-378-2635 >> >> This email and attachments contain information that may be confidential >> or privileged. If you are not the intended recipient, notify the sender >> at once and delete this message completely from your information system. >> Further use, disclosure, or copying of information contained in this >> email is not authorized, and any such action should not be construed as a >> waiver of privilege or other confidentiality protections. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 6 > Date: Tue, 17 Mar 2015 06:53:37 -0400 > From: "Jorge A. Santiago-Blay" > Subject: [Histonet] Comet assay: looking for reviewers of a short > paper > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Dear Histonetters: > > I serve as editor-in-chief of the peer-reviewed scientific journal, *Life: > The Excitement of Biology*. > > I just received a short (ca. 4,400 words long, incl. Literature Cited and > Figure Legends) paper that assesses DNA damage levels in a species > of mussel using the comet assay. If you are interested in serving as a > reviewer, please send me an email directly (= off the list) to > blayjorge@gmail.com. > > Gratefully, > > Jorge > > > Jorge A. Santiago-Blay, PhD > blaypublishers.com > > 1. Positive experiences for authors of papers published in *LEB* > http://blaypublishers.com/testimonials/ > > 2. Free examples of papers published in *LEB*: > http://blaypublishers.com/category/previous-issues/. > > 3. *Guidelines for Authors* and page charges of *LEB*: > http://blaypublishers.com/archives/ *.* > > 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ > > > http://blayjorge.wordpress.com/ > http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm > > > ------------------------------ > > Message: 7 > Date: Tue, 17 Mar 2015 12:38:00 +0000 > From: "Abbott, Tanya" > Subject: [Histonet] FW: Release of blocks to research facilities > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <852F7D2C14FB464D80E182B15DB138AF4CDCA504@CHIEX005.CHI.catholichealth.net> > > Content-Type: text/plain; charset="us-ascii" > > I did call the CAP and they stated first of all, that it is a very confusing checklist component! But I was told that the research facility is taking on the ownership of the block, and we need to ensure that whoever we are sending our blocks to are following the proper retention protocols. > > From: Abbott, Tanya > Sent: Monday, March 16, 2015 3:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Release of blocks to research facilities > > CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." > I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. > Thanks in advance for your help! Tanya > > Tanya G. Abbott > Manager Technologist > Histology/Cytology > St Joseph Medical Center > (phone) 610-378-2635 > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > > > ------------------------------ > > Message: 8 > Date: Tue, 17 Mar 2015 13:11:51 +0000 > From: "Boyd, Debbie M" > Subject: [Histonet] RE: Release of blocks to research facilities > To: "Abbott, Tanya" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > <7EAFE982E328304DA6CE2B677BB76246A9F0FF35@TN001WEXMBX014.US.chs.net> > Content-Type: text/plain; charset="us-ascii" > > I send them without hesitation. Ours is to assist the patient not get tangled up in regulatory bureaucracy. Just as you wouldn't hesitate to send a subpoenaed block. The point here is to KNOW where your blocks are when not on site. Yes we are custodians of blocks and slides, but you mustn't impede patient care. > > Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Abbott, Tanya [TanyaAbbott@catholichealth.net] > Sent: Monday, March 16, 2015 3:33 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Release of blocks to research facilities > > CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." > I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. > Thanks in advance for your help! Tanya > > Tanya G. Abbott > Manager Technologist > Histology/Cytology > St Joseph Medical Center > (phone) 610-378-2635 > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -------------------------------------------------------------------------- > Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. > > > > ------------------------------ > > Message: 9 > Date: Tue, 17 Mar 2015 09:47:55 -0400 > From: Fawn Bomar > Subject: [Histonet] ER/PR Benchmarks > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <0111BC10D77DC54EAB99B2DDA3BCE4B9832342@EXCH-2K10.hrhs.com> > Content-Type: text/plain; charset="iso-8859-1" > > > > > > > > > > > > > Hi all, > > > > &n > > > Does anyone know where to find the ER/PR benchmark numbers are to use to assess our annual numbers? > > > > > > Thank you > > > > Fawn > > > > > > > ----------------------------------- > This electronic message may contain inf > confidential or legally privileged. It is inten > for the use of the individual(s) and entity named as reci pients > > in the message. > > > > If you are not an inten > notify the sender immediate > computer. Do not deliver, dis and > > do not disclose its contents o > the information it contains. > > > Thank you > > > > > > ------------------------------ > > Message: 10 > Date: Tue, 17 Mar 2015 08:45:02 -0700 > From: WILLIAM DESALVO > Subject: Re: [Histonet] FW: Release of blocks to research facilities > To: "Abbott, Tanya" > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > There are other issues, besides CAP, that you should consider when releasing blocks. > 1. A block is a medical record. Has the patient consented to release for research? > 2. If the block is released prior to 14 days discharge and there are any charges, the charge must be sent to your hospital. Post 14 days the charges are sent to insurance and patient. > 3. Will the research lead to a commercial product? Again, written patient consent. > 4. You may be able to charge the research facility for sending the block. Administrative costs. > 5. Communicate with patient is a must. > 6. Get written consent and have the receiving facility verify they will handle properly, retain or return the block d you will get copies of data for patient file. > > Make sure you reduce your risk. > > Sent from my iPhone > >> On Mar 17, 2015, at 5:38 AM, Abbott, Tanya wrote: >> >> I did call the CAP and they stated first of all, that it is a very confusing checklist component! But I was told that the research facility is taking on the ownership of the block, and we need to ensure that whoever we are sending our blocks to are following the proper retention protocols. >> >> From: Abbott, Tanya >> Sent: Monday, March 16, 2015 3:33 PM >> To: 'histonet@lists.utsouthwestern.edu' >> Subject: Release of blocks to research facilities >> >> CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." >> I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. >> Thanks in advance for your help! Tanya >> >> Tanya G. Abbott >> Manager Technologist >> Histology/Cytology >> St Joseph Medical Center >> (phone) 610-378-2635 >> >> This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 11 > Date: Tue, 17 Mar 2015 16:18:26 +0000 > From: James Watson > Subject: RE: [Histonet] FW: Release of blocks to research facilities > To: 'WILLIAM DESALVO' , "Abbott, Tanya" > > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Bill is correct, CAP is actually the least of your worries. There are many regulations about human tissue used in research (I do not know them all), but patient consent is essential and in some cases the patient must actually agree to the specific research project. Here we have regulations about control block tissue that we use, we cannot get tissue directly from a local hospital. Also the laws are different in each country. There is a lot of bioethics involved. > > http://bioethics.od.nih.gov/humantissue.html > > Human Tissue Ownership and Use in Research: What Laboratorians and Researchers Should Know > http://www.clinchem.org/content/56/11/1675.full.pdf > > > > > James Watson HT ASCP > GNF Genomics Institute of the Novartis Research Foundation > Scientific Technical Leader II, Histology > Tel 858-332-4647 > Fax 858-812-1915 > jwatson@gnf.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Tuesday, March 17, 2015 8:45 AM > To: Abbott, Tanya > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] FW: Release of blocks to research facilities > > There are other issues, besides CAP, that you should consider when releasing blocks. > 1. A block is a medical record. Has the patient consented to release for research? > 2. If the block is released prior to 14 days discharge and there are any charges, the charge must be sent to your hospital. Post 14 days the charges are sent to insurance and patient. > 3. Will the research lead to a commercial product? Again, written patient consent. > 4. You may be able to charge the research facility for sending the block. Administrative costs. > 5. Communicate with patient is a must. > 6. Get written consent and have the receiving facility verify they will handle properly, retain or return the block d you will get copies of data for patient file. > > Make sure you reduce your risk. > > Sent from my iPhone > >> On Mar 17, 2015, at 5:38 AM, Abbott, Tanya wrote: >> >> I did call the CAP and they stated first of all, that it is a very confusing checklist component! But I was told that the research facility is taking on the ownership of the block, and we need to ensure that whoever we are sending our blocks to are following the proper retention protocols. >> >> From: Abbott, Tanya >> Sent: Monday, March 16, 2015 3:33 PM >> To: 'histonet@lists.utsouthwestern.edu' >> Subject: Release of blocks to research facilities >> >> CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." >> I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. >> Thanks in advance for your help! Tanya >> >> Tanya G. Abbott >> Manager Technologist >> Histology/Cytology >> St Joseph Medical Center >> (phone) 610-378-2635 >> >> This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 136, Issue 20 > ***************************************** From TanyaAbbott <@t> catholichealth.net Tue Mar 17 12:28:18 2015 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Tue Mar 17 12:29:37 2015 Subject: [Histonet] FW: Release of blocks to research facilities In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF4CDCA504@CHIEX005.CHI.catholichealth.net> Message-ID: <852F7D2C14FB464D80E182B15DB138AF4CDCAAAA@CHIEX005.CHI.catholichealth.net> Luckily we do have a research coordinator who takes care of all of these for us and he is very thorough. He obtains consent and deals with all costs. I am thinking that I just need to verify that the facility receiving the block (s) are retaining them for the appropriate amount of time, which they are. But yes, all good food for thought! Thanks for all the input folks! Tanya -----Original Message----- From: James Watson [mailto:JWatson@gnf.org] Sent: Tuesday, March 17, 2015 12:18 PM To: 'WILLIAM DESALVO'; Abbott, Tanya Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: Release of blocks to research facilities Bill is correct, CAP is actually the least of your worries. There are many regulations about human tissue used in research (I do not know them all), but patient consent is essential and in some cases the patient must actually agree to the specific research project. Here we have regulations about control block tissue that we use, we cannot get tissue directly from a local hospital. Also the laws are different in each country. There is a lot of bioethics involved. https://urldefense.proofpoint.com/v2/url?u=http-3A__bioethics.od.nih.gov_humantissue.html&d=AwIFAw&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=OCnLOvVFGUusxYsBbq-Ykuqe0CPoX1ZNX9HWkbBcgXE&s=RlbMe-0hfDNzmnp9qRfqYfeoqOM6X3Mg6YcKEkC3vl0&e= Human Tissue Ownership and Use in Research: What Laboratorians and Researchers Should Know https://urldefense.proofpoint.com/v2/url?u=http-3A__www.clinchem.org_content_56_11_1675.full.pdf&d=AwIFAw&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=OCnLOvVFGUusxYsBbq-Ykuqe0CPoX1ZNX9HWkbBcgXE&s=cyiM7Uax7JX_cIRMQBNYnrjVrhBa-8Aw3ekWJvXuuA0&e= James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Tuesday, March 17, 2015 8:45 AM To: Abbott, Tanya Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] FW: Release of blocks to research facilities There are other issues, besides CAP, that you should consider when releasing blocks. 1. A block is a medical record. Has the patient consented to release for research? 2. If the block is released prior to 14 days discharge and there are any charges, the charge must be sent to your hospital. Post 14 days the charges are sent to insurance and patient. 3. Will the research lead to a commercial product? Again, written patient consent. 4. You may be able to charge the research facility for sending the block. Administrative costs. 5. Communicate with patient is a must. 6. Get written consent and have the receiving facility verify they will handle properly, retain or return the block d you will get copies of data for patient file. Make sure you reduce your risk. Sent from my iPhone > On Mar 17, 2015, at 5:38 AM, Abbott, Tanya wrote: > > I did call the CAP and they stated first of all, that it is a very confusing checklist component! But I was told that the research facility is taking on the ownership of the block, and we need to ensure that whoever we are sending our blocks to are following the proper retention protocols. > > From: Abbott, Tanya > Sent: Monday, March 16, 2015 3:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Release of blocks to research facilities > > CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." > I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. > Thanks in advance for your help! Tanya > > Tanya G. Abbott > Manager Technologist > Histology/Cytology > St Joseph Medical Center > (phone) 610-378-2635 > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=AwIFAw&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0 > GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=OCnL > OvVFGUusxYsBbq-Ykuqe0CPoX1ZNX9HWkbBcgXE&s=2c-xmPLe0w9U7msIqGTexZcz1dzD > tm_PcYKs3J7CyGc&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=OCnLOvVFGUusxYsBbq-Ykuqe0CPoX1ZNX9HWkbBcgXE&s=2c-xmPLe0w9U7msIqGTexZcz1dzDtm_PcYKs3J7CyGc&e= From doug.porter <@t> caplab.org Tue Mar 17 12:40:42 2015 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Tue Mar 17 12:40:47 2015 Subject: [Histonet] Lead ( #3 ) In-Reply-To: References: <55085dc4.061cb60a.4f3a.ffff9d8dSMTPIN_ADDED_MISSING@mx.google.com> Message-ID: <000901d060d9$7e433d90$7ac9b8b0$@caplab.org> We have outfitted two labs with repurposed equipment that we purchased from Rankin. When there has been a problem with any of the equipment, it has been taken care of quickly. I'm not familiar with the other companies mentioned, but would recommend Rankin for your equipment needs. Perhaps you could even work out a deal on the shipping all the way North to Alaska?? Happy hunting. Douglas A. Porter, HT (ASCP) Pathologist Assistant IT Coordinator Sparrow / CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rob Rankin Sent: Tuesday, March 17, 2015 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lead ( #3 ) Here is a lead ( #3 ), but IMEB has a leg up with an endorsement already. Please ask Dawn Truscott or someone else to post a Rankin endorsement on Histonet ASAP and contact the prospect, Sent from my iPhone > On Mar 17, 2015, at 7:00 AM, histonet-request@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Histology position at CDC Atlanta > (Sanders, Jeanine (CDC/OID/NCEZID)) > 2. Refurbished Equipment (Leesa Jones) > 3. Re: Refurbished Equipment (Tina Van Meter) > 4. Release of blocks to research facilities (Abbott, Tanya) > 5. Re: Release of blocks to research facilities (Michael Ann Jones) > 6. Comet assay: looking for reviewers of a short paper > (Jorge A. Santiago-Blay) > 7. FW: Release of blocks to research facilities (Abbott, Tanya) > 8. RE: Release of blocks to research facilities (Boyd, Debbie M) > 9. ER/PR Benchmarks (Fawn Bomar) > 10. Re: FW: Release of blocks to research facilities (WILLIAM > DESALVO) 11. RE: FW: Release of blocks to research facilities (James > Watson) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 16 Mar 2015 17:20:52 +0000 > From: "Sanders, Jeanine (CDC/OID/NCEZID)" > Subject: [Histonet] Histology position at CDC Atlanta > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <3B2CD438E1628A41BD687E98B963B78137F0674B@EMBX-CLFT4.cdc.gov> > Content-Type: text/plain; charset="iso-8859-1" > > We are currently accepting resums for a Histotechnician, HT(ASCP) or Histotechnologist., HTL(ASCP) at the Centers for Disease Control and Prevention in Atlanta, GA. > > The ideal candidate will have a minimum of 2 years' experience in the histology lab to include processing, embedding, sectioning and both routine and special staining of slides. > > We have state-of-the-art instrumentation and are currently looking into a barcoding system. > > This is not an entry level position and is currently being offered as a contract position. > > If interested, please contact me with your resum and I will be happy to share more details. > > > Jeanine H. Sanders, BS HT(ASCP), QIHC > Centers for Disease Control and Prevention Infectious Diseases > Pathology Branch > 1600 Clifton Road, NE > MS/G-32 > Atlanta, Ga 30333 > 404-639-3590 > jqb7@cdc.gov > > > > > ------------------------------ > > Message: 2 > Date: Mon, 16 Mar 2015 09:23:05 -0800 > From: Leesa Jones > Subject: [Histonet] Refurbished Equipment > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=UTF-8 > > I am looking at purchasing a refurbished processor from either RBC, > GMI or IMEB. I would greatly appreciate any feedback/experiences > other Histonetter's have had with these companies. If there is another > company that you purchased refurbished equipment from and had a > wonderful experience with and would like to recommend them, I would > love to know. PM me at lcjones7@alaska.edu > > Thank you!!! > -- > *Leesa C. Jones* > > Animal Resources Center - Veterinary Services University of Alaska, > Fairbanks > 907-474-6054 or 907-474-7020 > lcjones7@alaska.edu > > > ------------------------------ > > Message: 3 > Date: Mon, 16 Mar 2015 13:53:47 -0400 > From: Tina Van Meter > Subject: Re: [Histonet] Refurbished Equipment > To: Leesa Jones > Cc: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset=UTF-8 > > Hi Leesa, > I have dealt with IMEB and have nothing but great things to say > concerning their equipment and customer service. The equipment was in > top notch condition and they contact me periodically to make sure > everything is working well. > > Good luck, > Tina > > T?ina Van Meter, HT (ASCP) > Histology Core Manager > Scripps Research Institute > Jupiter, Florida > >> On Mon, Mar 16, 2015 at 1:23 PM, Leesa Jones wrote: >> >> I am looking at purchasing a refurbished processor from either RBC, >> GMI or IMEB. I would greatly appreciate any feedback/experiences >> other Histonetter's have had with these companies. If there is >> another company that you purchased refurbished equipment from and had >> a wonderful experience with and would like to recommend them, I would >> love to know. PM me at lcjones7@alaska.edu >> >> Thank you!!! >> -- >> *Leesa C. Jones* >> >> Animal Resources Center - Veterinary Services University of Alaska, >> Fairbanks >> 907-474-6054 or 907-474-7020 >> lcjones7@alaska.edu >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 4 > Date: Mon, 16 Mar 2015 19:33:11 +0000 > From: "Abbott, Tanya" > Subject: [Histonet] Release of blocks to research facilities > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > <852F7D2C14FB464D80E182B15DB138AF4CDC9E6A@CHIEX005.CHI.catholichealth. > net> > > Content-Type: text/plain; charset="us-ascii" > > CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." > I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. > Thanks in advance for your help! Tanya > > Tanya G. Abbott > Manager Technologist > Histology/Cytology > St Joseph Medical Center > (phone) 610-378-2635 > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > > > ------------------------------ > > Message: 5 > Date: Mon, 16 Mar 2015 19:41:19 +0000 > From: Michael Ann Jones > Subject: Re: [Histonet] Release of blocks to research facilities > To: "Abbott, Tanya" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Following this closely. . . > Michael Ann > Michael Ann Jones, HT (ASCP) > Histology Manager > Metropath > 7444 W. Alaska Dr. #250 > Lakewood, CO 80226 > 303.634.2511 > Mjones@metropath.com > > > > > > > On 3/16/15, 1:33 PM, "Abbott, Tanya" > wrote: > >> CAP checklist ANP.12500 refers to "Record Retention". I am looking >> specifically at NOTE 2: "Regarding extra-institutional release of >> blocks for research purposes." >> I am wondering how everyone handles this, especially if you have only >> 1 block on newly diagnosed patient and the Doctor wants it sent out >> for research. >> Thanks in advance for your help! Tanya >> >> Tanya G. Abbott >> Manager Technologist >> Histology/Cytology >> St Joseph Medical Center >> (phone) 610-378-2635 >> >> This email and attachments contain information that may be >> confidential or privileged. If you are not the intended recipient, >> notify the sender at once and delete this message completely from your information system. >> Further use, disclosure, or copying of information contained in this >> email is not authorized, and any such action should not be construed >> as a waiver of privilege or other confidentiality protections. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 6 > Date: Tue, 17 Mar 2015 06:53:37 -0400 > From: "Jorge A. Santiago-Blay" > Subject: [Histonet] Comet assay: looking for reviewers of a short > paper > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=UTF-8 > > Dear Histonetters: > > I serve as editor-in-chief of the peer-reviewed scientific journal, *Life: > The Excitement of Biology*. > > I just received a short (ca. 4,400 words long, incl. Literature Cited > and Figure Legends) paper that assesses DNA damage levels in a species > of mussel using the comet assay. If you are interested in serving as a > reviewer, please send me an email directly (= off the list) to > blayjorge@gmail.com. > > Gratefully, > > Jorge > > > Jorge A. Santiago-Blay, PhD > blaypublishers.com > > 1. Positive experiences for authors of papers published in *LEB* > http://blaypublishers.com/testimonials/ > > 2. Free examples of papers published in *LEB*: > http://blaypublishers.com/category/previous-issues/. > > 3. *Guidelines for Authors* and page charges of *LEB*: > http://blaypublishers.com/archives/ *.* > > 4. Want to subscribe to *LEB*? > http://blaypublishers.com/subscriptions/ > > > http://blayjorge.wordpress.com/ > http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm > > > ------------------------------ > > Message: 7 > Date: Tue, 17 Mar 2015 12:38:00 +0000 > From: "Abbott, Tanya" > Subject: [Histonet] FW: Release of blocks to research facilities > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > <852F7D2C14FB464D80E182B15DB138AF4CDCA504@CHIEX005.CHI.catholichealth. > net> > > Content-Type: text/plain; charset="us-ascii" > > I did call the CAP and they stated first of all, that it is a very confusing checklist component! But I was told that the research facility is taking on the ownership of the block, and we need to ensure that whoever we are sending our blocks to are following the proper retention protocols. > > From: Abbott, Tanya > Sent: Monday, March 16, 2015 3:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Release of blocks to research facilities > > CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." > I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. > Thanks in advance for your help! Tanya > > Tanya G. Abbott > Manager Technologist > Histology/Cytology > St Joseph Medical Center > (phone) 610-378-2635 > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > > > ------------------------------ > > Message: 8 > Date: Tue, 17 Mar 2015 13:11:51 +0000 > From: "Boyd, Debbie M" > Subject: [Histonet] RE: Release of blocks to research facilities > To: "Abbott, Tanya" , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > <7EAFE982E328304DA6CE2B677BB76246A9F0FF35@TN001WEXMBX014.US.chs.net> > Content-Type: text/plain; charset="us-ascii" > > I send them without hesitation. Ours is to assist the patient not get tangled up in regulatory bureaucracy. Just as you wouldn't hesitate to send a subpoenaed block. The point here is to KNOW where your blocks are when not on site. Yes we are custodians of blocks and slides, but you mustn't impede patient care. > > Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional > Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | > PH 804-765-5025 | FAX 804-765-6058 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] on behalf of Abbott, Tanya > [TanyaAbbott@catholichealth.net] > Sent: Monday, March 16, 2015 3:33 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Release of blocks to research facilities > > CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." > I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. > Thanks in advance for your help! Tanya > > Tanya G. Abbott > Manager Technologist > Histology/Cytology > St Joseph Medical Center > (phone) 610-378-2635 > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ---------------------------------------------------------------------- > ---- > Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. > > > > ------------------------------ > > Message: 9 > Date: Tue, 17 Mar 2015 09:47:55 -0400 > From: Fawn Bomar > Subject: [Histonet] ER/PR Benchmarks > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <0111BC10D77DC54EAB99B2DDA3BCE4B9832342@EXCH-2K10.hrhs.com> > Content-Type: text/plain; charset="iso-8859-1" > > > > > > > > > > > > > Hi all, > > > > &n > > > Does anyone know where to find the ER/PR benchmark numbers are to use to assess our annual numbers? > > > > > > Thank you > > > > Fawn > > > > > > > ----------------------------------- > This electronic message may contain inf > confidential or legally privileged. It is inten > for the use of the individual(s) and entity named as reci pients > > in the message. > > > > If you are not an inten > notify the sender immediate > computer. Do not deliver, dis and > > do not disclose its contents o > the information it contains. > > > Thank you > > > > > > ------------------------------ > > Message: 10 > Date: Tue, 17 Mar 2015 08:45:02 -0700 > From: WILLIAM DESALVO > Subject: Re: [Histonet] FW: Release of blocks to research facilities > To: "Abbott, Tanya" > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > There are other issues, besides CAP, that you should consider when releasing blocks. > 1. A block is a medical record. Has the patient consented to release for research? > 2. If the block is released prior to 14 days discharge and there are any charges, the charge must be sent to your hospital. Post 14 days the charges are sent to insurance and patient. > 3. Will the research lead to a commercial product? Again, written patient consent. > 4. You may be able to charge the research facility for sending the block. Administrative costs. > 5. Communicate with patient is a must. > 6. Get written consent and have the receiving facility verify they will handle properly, retain or return the block d you will get copies of data for patient file. > > Make sure you reduce your risk. > > Sent from my iPhone > >> On Mar 17, 2015, at 5:38 AM, Abbott, Tanya wrote: >> >> I did call the CAP and they stated first of all, that it is a very confusing checklist component! But I was told that the research facility is taking on the ownership of the block, and we need to ensure that whoever we are sending our blocks to are following the proper retention protocols. >> >> From: Abbott, Tanya >> Sent: Monday, March 16, 2015 3:33 PM >> To: 'histonet@lists.utsouthwestern.edu' >> Subject: Release of blocks to research facilities >> >> CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." >> I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. >> Thanks in advance for your help! Tanya >> >> Tanya G. Abbott >> Manager Technologist >> Histology/Cytology >> St Joseph Medical Center >> (phone) 610-378-2635 >> >> This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 11 > Date: Tue, 17 Mar 2015 16:18:26 +0000 > From: James Watson > Subject: RE: [Histonet] FW: Release of blocks to research facilities > To: 'WILLIAM DESALVO' , "Abbott, Tanya" > > Cc: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Bill is correct, CAP is actually the least of your worries. There are many regulations about human tissue used in research (I do not know them all), but patient consent is essential and in some cases the patient must actually agree to the specific research project. Here we have regulations about control block tissue that we use, we cannot get tissue directly from a local hospital. Also the laws are different in each country. There is a lot of bioethics involved. > > http://bioethics.od.nih.gov/humantissue.html > > Human Tissue Ownership and Use in Research: What Laboratorians and > Researchers Should Know > http://www.clinchem.org/content/56/11/1675.full.pdf > > > > > James Watson HT ASCP > GNF Genomics Institute of the Novartis Research Foundation Scientific > Technical Leader II, Histology > Tel 858-332-4647 > Fax 858-812-1915 > jwatson@gnf.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > WILLIAM DESALVO > Sent: Tuesday, March 17, 2015 8:45 AM > To: Abbott, Tanya > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] FW: Release of blocks to research facilities > > There are other issues, besides CAP, that you should consider when releasing blocks. > 1. A block is a medical record. Has the patient consented to release for research? > 2. If the block is released prior to 14 days discharge and there are any charges, the charge must be sent to your hospital. Post 14 days the charges are sent to insurance and patient. > 3. Will the research lead to a commercial product? Again, written patient consent. > 4. You may be able to charge the research facility for sending the block. Administrative costs. > 5. Communicate with patient is a must. > 6. Get written consent and have the receiving facility verify they will handle properly, retain or return the block d you will get copies of data for patient file. > > Make sure you reduce your risk. > > Sent from my iPhone > >> On Mar 17, 2015, at 5:38 AM, Abbott, Tanya wrote: >> >> I did call the CAP and they stated first of all, that it is a very confusing checklist component! But I was told that the research facility is taking on the ownership of the block, and we need to ensure that whoever we are sending our blocks to are following the proper retention protocols. >> >> From: Abbott, Tanya >> Sent: Monday, March 16, 2015 3:33 PM >> To: 'histonet@lists.utsouthwestern.edu' >> Subject: Release of blocks to research facilities >> >> CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." >> I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. >> Thanks in advance for your help! Tanya >> >> Tanya G. Abbott >> Manager Technologist >> Histology/Cytology >> St Joseph Medical Center >> (phone) 610-378-2635 >> >> This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 136, Issue 20 > ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Tue Mar 17 13:00:07 2015 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Mar 17 13:00:23 2015 Subject: [Histonet] RE: Release of blocks to research facilities In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF4CDC9E6A@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF4CDC9E6A@CHIEX005.CHI.catholichealth.net> Message-ID: The notes say that after two years it can be released if approved, but the best way is to intentionally preserve material if you have any idea there may be a need. And we could provide unstained slides. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Monday, March 16, 2015 3:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Release of blocks to research facilities CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. Thanks in advance for your help! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From b-frederick <@t> northwestern.edu Tue Mar 17 13:51:42 2015 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Mar 17 13:51:47 2015 Subject: [Histonet] FW: Release of blocks to research facilities In-Reply-To: References: <852F7D2C14FB464D80E182B15DB138AF4CDCA504@CHIEX005.CHI.catholichealth.net> Message-ID: <8336d55bcb1b4f7990aa7de1d9a4da06@evcspmbx03.ads.northwestern.edu> Research facilities usually pay the submitting institution for submitting the block to the study. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Tuesday, March 17, 2015 10:45 AM To: Abbott, Tanya Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] FW: Release of blocks to research facilities There are other issues, besides CAP, that you should consider when releasing blocks. 1. A block is a medical record. Has the patient consented to release for research? 2. If the block is released prior to 14 days discharge and there are any charges, the charge must be sent to your hospital. Post 14 days the charges are sent to insurance and patient. 3. Will the research lead to a commercial product? Again, written patient consent. 4. You may be able to charge the research facility for sending the block. Administrative costs. 5. Communicate with patient is a must. 6. Get written consent and have the receiving facility verify they will handle properly, retain or return the block d you will get copies of data for patient file. Make sure you reduce your risk. Sent from my iPhone > On Mar 17, 2015, at 5:38 AM, Abbott, Tanya wrote: > > I did call the CAP and they stated first of all, that it is a very confusing checklist component! But I was told that the research facility is taking on the ownership of the block, and we need to ensure that whoever we are sending our blocks to are following the proper retention protocols. > > From: Abbott, Tanya > Sent: Monday, March 16, 2015 3:33 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Release of blocks to research facilities > > CAP checklist ANP.12500 refers to "Record Retention". I am looking specifically at NOTE 2: "Regarding extra-institutional release of blocks for research purposes." > I am wondering how everyone handles this, especially if you have only 1 block on newly diagnosed patient and the Doctor wants it sent out for research. > Thanks in advance for your help! Tanya > > Tanya G. Abbott > Manager Technologist > Histology/Cytology > St Joseph Medical Center > (phone) 610-378-2635 > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ddsterchi <@t> comcast.net Tue Mar 17 14:01:51 2015 From: ddsterchi <@t> comcast.net (Dennis & Diane Sterchi) Date: Tue Mar 17 14:02:20 2015 Subject: [Histonet] refurbish equipment Message-ID: <3779BCAF-7ABA-49BB-BE48-BEE8AB4EF302@comcast.net> Hi Leesa Over the years, I have bought a lot of used equipment from IMEB and was really happy with all of it. They have access to good used equipment and have the people to get it to you on time and experts using the equipment. They also have consultants that will help you decide what equipment you may need (or not need) to do the job you want done. Please email me if you want more information. Good luck in your future purchases. Regards Diane Sterchi From BZIMMERM <@t> gru.edu Tue Mar 17 14:48:42 2015 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Tue Mar 17 14:48:48 2015 Subject: [Histonet] HISTOPALOOZA GSH 2015 CALL FOR ALL PROCRASTINATORS!! Message-ID: Today is the last day to reserve your room at Legacy Lodge at Lake Lanier Islands. Plan to join us and get your "PALOOZA" on~ Bring a swimsuit for the heated pool, get ready for a game of pool, join us at the Bullfrog lounge or by the fire pit for s'mores. Education, fun, networking, exhibits and "HISTOPALOOZING" www.histosearch.com/gsh/ Wanda K Simons, HT ASCP GSH President From patpxs <@t> gmail.com Tue Mar 17 17:51:26 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Tue Mar 17 17:51:30 2015 Subject: [Histonet] GMS controls? Message-ID: Good Afternoon Netters, I have a project to validate a special stainer and will be starting with GMS. What can I use as a good source of fungus? Was it last week there was mention of orange peels and onions? I need to get the OK of the attending pathologist, so I probably can't use anything too funky, like fruits and vegetables. I don't have access to fresh lung tissue to smear cream cheese on (also mentioned last week) so I was hoping some type of easy to buy processed food (like Slim Jims from Gram stain) is out there to be used as a fungal control. Ideas? Thanks in advance. Paula :-) From pdefazio802 <@t> gmail.com Tue Mar 17 20:44:30 2015 From: pdefazio802 <@t> gmail.com (Pam DeFazio) Date: Tue Mar 17 20:44:34 2015 Subject: [Histonet] Tyvek arm sleeves Message-ID: Anyone having a problem with little to no elastic in these sleeves? Seems to be "hit and miss ". I can't pin it on a particular lot #. From PAMarcum <@t> uams.edu Wed Mar 18 07:54:52 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Mar 18 07:54:58 2015 Subject: [Histonet] Posting for a Friend Message-ID: <77fef61f8e96470da78bc4190f10f91a@MAIL13M2N2.ad.uams.edu> If you are interested in this position please contact Nephropath directly. Nephropath is seeking a manager for its CAP accredited laboratory. Prospective candidates should hold a bachelor's degree as well as HTL or MT certification and have a minimum of five years of experience supervising in an accredited histology laboratory. The successful candidate will have proven technical and supervisory experience and exhibit an ability to exceed service, quality, and staff expectations. Salary will be contingent on a combination of qualification and experience. Nephropath is a rapidly growing pathology practice founded in 2001 and located in Little Rock, AR. The cornerstone of the practice is providing same-day results for renal biopsies but is rapidly expanding into other pathology specialties and clinical testing. In 2014 Nephropath processed more than 10,000 renal pathology cases. If interested, please send your resume or questions to aaron.nichols@nephropath.com Thank You, Pam Marcum ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Kari.Breal <@t> alexian.net Wed Mar 18 09:13:03 2015 From: Kari.Breal <@t> alexian.net (Breal, Kari) Date: Wed Mar 18 09:13:14 2015 Subject: [Histonet] Releasing specimens to patients Message-ID: Does anyone have a procedure and consent form they would be willing to share with me regarding the release of specimens to the patient? We are revising our whole process and I'm interested in what others are doing. Thank you kindly! Kari H. Breal, HT (ASCP) Histology Manager ABMC- 847-437-5500 x 5155 SAMC- 847-843-2000 x 6818 Kari.Breal@alexian.net CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. From Sherrian.McAnn <@t> va.gov Wed Mar 18 11:57:36 2015 From: Sherrian.McAnn <@t> va.gov (McAnn, Sherrian) Date: Wed Mar 18 11:58:01 2015 Subject: [EXTERNAL] [Histonet] GMS controls? In-Reply-To: References: Message-ID: <61E2B58CECEF384094A363989D47C0900BF54CD8@VHAV17MSGA2.v17.med.va.gov> I have experimented on some things and found Blue Cheese to be excellent source of not only fungus but yeast and some bacteria. I did make a couple of smears with the blue cheese but seemed to do much better after just routine processing some of the crumbles of blue cheese in cassettes. Sincerely, Sherrian McAnn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, March 17, 2015 5:51 PM To: HistoNet Subject: [EXTERNAL] [Histonet] GMS controls? Good Afternoon Netters, I have a project to validate a special stainer and will be starting with GMS. What can I use as a good source of fungus? Was it last week there was mention of orange peels and onions? I need to get the OK of the attending pathologist, so I probably can't use anything too funky, like fruits and vegetables. I don't have access to fresh lung tissue to smear cream cheese on (also mentioned last week) so I was hoping some type of easy to buy processed food (like Slim Jims from Gram stain) is out there to be used as a fungal control. Ideas? Thanks in advance. Paula :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jonathan.Arzt <@t> ARS.USDA.GOV Wed Mar 18 12:08:19 2015 From: Jonathan.Arzt <@t> ARS.USDA.GOV (Arzt, Jonathan) Date: Wed Mar 18 12:09:05 2015 Subject: [Histonet] HT Job at Plum Island - JUST 2 DAYS LEFT In-Reply-To: <7213AC15544DE945843D821E1769E38A06A1CC05@001FSN2MPN1-053.001f.mgd2.msft.net> References: <7213AC15544DE945843D821E1769E38A06A1CC05@001FSN2MPN1-053.001f.mgd2.msft.net> Message-ID: <7213AC15544DE945843D821E1769E38A06A299E2@001FSN2MPN1-052.001f.mgd2.msft.net> Immediately available and open for application for JUST 2 DAYS, is a full-time, permanent, federal histotchnologist position with the Foreign Animal Disease Research Unit at the Plum Island Animal Disease Center. Job Title: Biologist (Histotechnologist) Department: Department Of Agriculture Agency: Agricultural Research Service Job Announcement Number: ARS-S15E-0045 SALARY RANGE: $44,617.00 to $85,841.00 / Per Year See the full posting and application details here: https://www.usajobs.gov/GetJob/ViewDetails/396084800 From may <@t> dbiosys.com Wed Mar 18 12:22:11 2015 From: may <@t> dbiosys.com (May Wei) Date: Wed Mar 18 12:22:42 2015 Subject: [Histonet] Used Tissue Embedding Station Message-ID: I am looking for used Tissue Embedding Station. If you know anyone who might offer, please contact me. Thank you and regards, May May Wei, M.Med., MBA Director, International Sales Diagnostic BioSystems 6616 Owens Drive, Pleasanton, CA 94588, USA Tel: +1 925-484-3350 Mobile: +1 415-812-8095 Toll: +1 888-896-3350 Fax: +1 925-484-3390 E-mail: may@dbiosys.com URL: www.dbiosys.com From sally.norton <@t> seattlechildrens.org Wed Mar 18 12:29:07 2015 From: sally.norton <@t> seattlechildrens.org (Norton, Sally) Date: Wed Mar 18 12:29:12 2015 Subject: [EXTERNAL] [Histonet] GMS controls? In-Reply-To: <61E2B58CECEF384094A363989D47C0900BF54CD8@VHAV17MSGA2.v17.med.va.gov> References: <61E2B58CECEF384094A363989D47C0900BF54CD8@VHAV17MSGA2.v17.med.va.gov> Message-ID: <1357F84B33D39A46BA015A8EC6ABCBD040EB630B@PPWEXD01a.childrens.sea.kids> We used Brie that we let grow mold for fungus. Sally Norton Histo Tech Seattle children's Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian Sent: Wednesday, March 18, 2015 9:58 AM To: Paula Sicurello; HistoNet Subject: RE: [EXTERNAL] [Histonet] GMS controls? I have experimented on some things and found Blue Cheese to be excellent source of not only fungus but yeast and some bacteria. I did make a couple of smears with the blue cheese but seemed to do much better after just routine processing some of the crumbles of blue cheese in cassettes. Sincerely, Sherrian McAnn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, March 17, 2015 5:51 PM To: HistoNet Subject: [EXTERNAL] [Histonet] GMS controls? Good Afternoon Netters, I have a project to validate a special stainer and will be starting with GMS. What can I use as a good source of fungus? Was it last week there was mention of orange peels and onions? I need to get the OK of the attending pathologist, so I probably can't use anything too funky, like fruits and vegetables. I don't have access to fresh lung tissue to smear cream cheese on (also mentioned last week) so I was hoping some type of easy to buy processed food (like Slim Jims from Gram stain) is out there to be used as a fungal control. Ideas? Thanks in advance. Paula :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From michael <@t> mcevoyandfarmer.com Wed Mar 18 12:31:17 2015 From: michael <@t> mcevoyandfarmer.com (Michael Farmer) Date: Wed Mar 18 12:31:20 2015 Subject: [Histonet] Dako Omnis Message-ID: I would appreciate hearing from anyone who has (or has seriously considered) an Omnis. Just a brief sentence or two on how it's going would be nice. Much obliged Michael Farmer McEvoy & Farmer Pathology michael@mcevoyandfarmer.com cell 415-994-8852 From Sarah_Mack <@t> urmc.rochester.edu Wed Mar 18 12:50:02 2015 From: Sarah_Mack <@t> urmc.rochester.edu (Mack, Sarah) Date: Wed Mar 18 12:50:35 2015 Subject: [Histonet] NYSHS 2015 Corporate Educational Scholarships Message-ID: The Awards Committee, formed from the Officers and Directors of the society, carefully evaluate all nominees and supporting documentation submitted for the scholarships. Recipients are presented their award at the New York State Histotechnological Society annual spring meeting. To be eligible, please send an application to the Awards Chairperson specifying the award you are applying for and include the following items: You must be a member in good standing (the current year). A letter from you showing evidence of your commitment to the profession. One letter of recommendation from a supervisor, pathologist, histotechnologist or professor. Name and address of your current employer or school, your current mailing address and email address. Applications are due March 31st, 2015. We prefer email submission of applications and letters of recommendation. Please specify which awards you are applying for in your correspondence. Please send documents to: Angela M. Fogg, HT ASCP angelafogg@aol.com Mailing address: 5 Hammersley Ave Poughkeepsie, NY 12601 2015 Scholarships Gulf Coast Instrument Company: Is sponsoring a $250 scholarship. The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The awards must be used to defray educational expenses. Mercedes Medical Award: The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The $250 award is sponsored by Mercedes Medical and must be used to defray educational expenses. Leica Biosystems Award: The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The $250 award is sponsored by Leica Biosystems and must be used to defray educational expenses. Sakura Finetek: The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The $250 award is sponsored by Sakura Finetek USA and must be used to defray educational expenses. Source Medical Products Award: The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The $250 award is sponsored by Source Medical Products and must be used to defray educational expenses. StatLab Medical Products Award: The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The $250 award is sponsored by StatLab Medical Products and must be used to defray educational expenses. 20 people reached New York State Histotechnological Society Like ? Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research Histology, Biochemistry, and Molecular Imaging Core 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-3901 From sforeman <@t> labpath.com Wed Mar 18 13:03:24 2015 From: sforeman <@t> labpath.com (Susan Foreman) Date: Wed Mar 18 13:07:20 2015 Subject: [Histonet] Dako Omnis In-Reply-To: References: Message-ID: <010201d061a5$d3f23b70$7bd6b250$@labpath.com> I would also like to hear any information that anyone has gathered. Many Thanks, Susan Foreman sforeman@labpath.com 865-584-1933 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Farmer Sent: Wednesday, March 18, 2015 1:31 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Omnis I would appreciate hearing from anyone who has (or has seriously considered) an Omnis. Just a brief sentence or two on how it's going would be nice. Much obliged Michael Farmer McEvoy & Farmer Pathology michael@mcevoyandfarmer.com cell 415-994-8852 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anna.c.hughes <@t> gsk.com Wed Mar 18 14:01:06 2015 From: anna.c.hughes <@t> gsk.com (Anna Hughes) Date: Wed Mar 18 14:01:18 2015 Subject: [Histonet] sarcomatous mesothelioma Message-ID: <89e95372aa5f466c8fd3654cbb47bf3e@BLUPR66MB0051.019D.MGD.MSFT.NET> Hi Everyone! I am wondering what antibodies everyone uses to identify mesotheliomas, specifically sarcomatous mesothelioma and if there is a negative antibody you use to confirm. Thanks! Anna Anna C. Hughes, HTL (ASCP), QIHC Senior Scientist, Morphologic Pathology anna.c.hughes@gsk.com From Lacie.Algeo <@t> providence.org Wed Mar 18 15:15:48 2015 From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A) Date: Wed Mar 18 15:16:26 2015 Subject: [Histonet] bone marrow billing fees Message-ID: <24C4B3C167E5694887AB594C7602CE3A03BAB8A0@WN35104.or.providence.org> Hi Everyone, I was wondering if anyone is billing for a Histo technical charge on bone marrow aspirates and biopsies (i.e. an 88305) in addition to all of the Hematology fee codes. Our Histology department does the grossing, embedding and microtomy for all bone marrow blocks, however we do not bill for/receive any revenue for this work. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From TanyaAbbott <@t> catholichealth.net Wed Mar 18 15:17:38 2015 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Wed Mar 18 15:17:46 2015 Subject: [Histonet] ANP.22760 New Reagent Lot Confirmation of Acceptability Message-ID: <852F7D2C14FB464D80E182B15DB138AF4CDCBAFD@CHIEX005.CHI.catholichealth.net> Can you tell it's "review the CAP checklist time"?!! In regards to ANP.22760, New Reagent Lot Confirmation of Acceptability, how does everyone handle it? I have a procedure in place, but I would like it to flow a bit better. Thanks in advance! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From Bonnie.Whitaker <@t> osumc.edu Wed Mar 18 15:45:16 2015 From: Bonnie.Whitaker <@t> osumc.edu (Whitaker, Bonnie) Date: Wed Mar 18 15:45:29 2015 Subject: [Histonet] RE: bone marrow billing fees In-Reply-To: <24C4B3C167E5694887AB594C7602CE3A03BAB8A0@WN35104.or.providence.org> References: <24C4B3C167E5694887AB594C7602CE3A03BAB8A0@WN35104.or.providence.org> Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670E14E23113@EXMBOX-VP05.OSUMC.EDU> Hi Lacie, Anything that is grossed, embedded and has slides cut and stained by histology (core bx or clot) should be billed an 88305. Bonnie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A Sent: Wednesday, March 18, 2015 4:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone marrow billing fees Hi Everyone, I was wondering if anyone is billing for a Histo technical charge on bone marrow aspirates and biopsies (i.e. an 88305) in addition to all of the Hematology fee codes. Our Histology department does the grossing, embedding and microtomy for all bone marrow blocks, however we do not bill for/receive any revenue for this work. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=qJ2DbHovRUIxlF1AnAt9XBNpD_BCv8Upx8EJDnWyG5s&s=dM8BUM3L49UUcAoPk9kwSmVe3om4g-NO9Gw-GKfDGCQ&e= From nto <@t> stowers.org Thu Mar 19 12:21:26 2015 From: nto <@t> stowers.org (Thomas, Nancy) Date: Thu Mar 19 12:21:37 2015 Subject: [Histonet] preparing a 20% polyvinyl alcohol solution Message-ID: Dear Histonet, I am making a 20% PVA solution for cryo embedding murine bones, following John Tarpley's recipe found in his JOH article, March 2003. He mentions no problems in making it, but after 3 days on the stir plate, it still has not gone into solution. The PVA settles very quickly, even before I can pour it into a mold to freeze and test. Is there a trick to this that I need to know? Any advice would be so great. Thank you, Nancy Thomas From Lacie.Algeo <@t> providence.org Thu Mar 19 12:32:04 2015 From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A) Date: Thu Mar 19 12:32:44 2015 Subject: [Histonet] Fungus control Message-ID: <24C4B3C167E5694887AB594C7602CE3A03BABB11@WN35104.or.providence.org> We used an orange. Leave it in a brown bag until it grows fungus. Then you can cut strips of the peel and process just like your regular tissue. You get all the hyphae nicely defined in the peel. The counterstain doesn't really show, though, since it is plant cells. Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, March 19, 2015 10:06 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 136, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. HT Job at Plum Island - JUST 2 DAYS LEFT (Arzt, Jonathan) 2. Used Tissue Embedding Station (May Wei) 3. RE: [EXTERNAL] [Histonet] GMS controls? (Norton, Sally) 4. Dako Omnis (Michael Farmer) 5. NYSHS 2015 Corporate Educational Scholarships (Mack, Sarah) 6. RE: Dako Omnis (Susan Foreman) 7. sarcomatous mesothelioma (Anna Hughes) 8. bone marrow billing fees (Algeo, Lacie A) 9. ANP.22760 New Reagent Lot Confirmation of Acceptability (Abbott, Tanya) 10. RE: bone marrow billing fees (Whitaker, Bonnie) ---------------------------------------------------------------------- Message: 1 Date: Wed, 18 Mar 2015 17:08:19 +0000 From: "Arzt, Jonathan" Subject: [Histonet] HT Job at Plum Island - JUST 2 DAYS LEFT To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <7213AC15544DE945843D821E1769E38A06A299E2@001FSN2MPN1-052.001f.mgd2.msft.net> Content-Type: text/plain; charset="us-ascii" Immediately available and open for application for JUST 2 DAYS, is a full-time, permanent, federal histotchnologist position with the Foreign Animal Disease Research Unit at the Plum Island Animal Disease Center. Job Title: Biologist (Histotechnologist) Department: Department Of Agriculture Agency: Agricultural Research Service Job Announcement Number: ARS-S15E-0045 SALARY RANGE: $44,617.00 to $85,841.00 / Per Year See the full posting and application details here: https://www.usajobs.gov/GetJob/ViewDetails/396084800 ------------------------------ Message: 2 Date: Wed, 18 Mar 2015 17:22:11 +0000 From: May Wei Subject: [Histonet] Used Tissue Embedding Station To: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I am looking for used Tissue Embedding Station. If you know anyone who might offer, please contact me. Thank you and regards, May May Wei, M.Med., MBA Director, International Sales Diagnostic BioSystems 6616 Owens Drive, Pleasanton, CA 94588, USA Tel: +1 925-484-3350 Mobile: +1 415-812-8095 Toll: +1 888-896-3350 Fax: +1 925-484-3390 E-mail: may@dbiosys.com URL: www.dbiosys.com ------------------------------ Message: 3 Date: Wed, 18 Mar 2015 17:29:07 +0000 From: "Norton, Sally" Subject: RE: [EXTERNAL] [Histonet] GMS controls? To: "'McAnn, Sherrian'" , Paula Sicurello , HistoNet Message-ID: <1357F84B33D39A46BA015A8EC6ABCBD040EB630B@PPWEXD01a.childrens.sea.kids> Content-Type: text/plain; charset="utf-8" We used Brie that we let grow mold for fungus. Sally Norton Histo Tech Seattle children's Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian Sent: Wednesday, March 18, 2015 9:58 AM To: Paula Sicurello; HistoNet Subject: RE: [EXTERNAL] [Histonet] GMS controls? I have experimented on some things and found Blue Cheese to be excellent source of not only fungus but yeast and some bacteria. I did make a couple of smears with the blue cheese but seemed to do much better after just routine processing some of the crumbles of blue cheese in cassettes. Sincerely, Sherrian McAnn -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, March 17, 2015 5:51 PM To: HistoNet Subject: [EXTERNAL] [Histonet] GMS controls? Good Afternoon Netters, I have a project to validate a special stainer and will be starting with GMS. What can I use as a good source of fungus? Was it last week there was mention of orange peels and onions? I need to get the OK of the attending pathologist, so I probably can't use anything too funky, like fruits and vegetables. I don't have access to fresh lung tissue to smear cream cheese on (also mentioned last week) so I was hoping some type of easy to buy processed food (like Slim Jims from Gram stain) is out there to be used as a fungal control. Ideas? Thanks in advance. Paula :-) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 4 Date: Wed, 18 Mar 2015 13:31:17 -0400 From: Michael Farmer Subject: [Histonet] Dako Omnis To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii I would appreciate hearing from anyone who has (or has seriously considered) an Omnis. Just a brief sentence or two on how it's going would be nice. Much obliged Michael Farmer McEvoy & Farmer Pathology michael@mcevoyandfarmer.com cell 415-994-8852 ------------------------------ Message: 5 Date: Wed, 18 Mar 2015 13:50:02 -0400 From: "Mack, Sarah" Subject: [Histonet] NYSHS 2015 Corporate Educational Scholarships To: "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" The Awards Committee, formed from the Officers and Directors of the society, carefully evaluate all nominees and supporting documentation submitted for the scholarships. Recipients are presented their award at the New York State Histotechnological Society annual spring meeting. To be eligible, please send an application to the Awards Chairperson specifying the award you are applying for and include the following items: You must be a member in good standing (the current year). A letter from you showing evidence of your commitment to the profession. One letter of recommendation from a supervisor, pathologist, histotechnologist or professor. Name and address of your current employer or school, your current mailing address and email address. Applications are due March 31st, 2015. We prefer email submission of applications and letters of recommendation. Please specify which awards you are applying for in your correspondence. Please send documents to: Angela M. Fogg, HT ASCP angelafogg@aol.com Mailing address: 5 Hammersley Ave Poughkeepsie, NY 12601 2015 Scholarships Gulf Coast Instrument Company: Is sponsoring a $250 scholarship. The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The awards must be used to defray educational expenses. Mercedes Medical Award: The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The $250 award is sponsored by Mercedes Medical and must be used to defray educational expenses. Leica Biosystems Award: The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The $250 award is sponsored by Leica Biosystems and must be used to defray educational expenses. Sakura Finetek: The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The $250 award is sponsored by Sakura Finetek USA and must be used to defray educational expenses. Source Medical Products Award: The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The $250 award is sponsored by Source Medical Products and must be used to defray educational expenses. StatLab Medical Products Award: The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The $250 award is sponsored by StatLab Medical Products and must be used to defray educational expenses. 20 people reached New York State Histotechnological Society Like ? Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research Histology, Biochemistry, and Molecular Imaging Core 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-3901 ------------------------------ Message: 6 Date: Wed, 18 Mar 2015 14:03:24 -0400 From: "Susan Foreman" Subject: RE: [Histonet] Dako Omnis To: "'Michael Farmer'" , Message-ID: <010201d061a5$d3f23b70$7bd6b250$@labpath.com> Content-Type: text/plain; charset="us-ascii" I would also like to hear any information that anyone has gathered. Many Thanks, Susan Foreman sforeman@labpath.com 865-584-1933 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Farmer Sent: Wednesday, March 18, 2015 1:31 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Omnis I would appreciate hearing from anyone who has (or has seriously considered) an Omnis. Just a brief sentence or two on how it's going would be nice. Much obliged Michael Farmer McEvoy & Farmer Pathology michael@mcevoyandfarmer.com cell 415-994-8852 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 18 Mar 2015 19:01:06 +0000 From: Anna Hughes Subject: [Histonet] sarcomatous mesothelioma To: "histonet@lists.utsouthwestern.edu" Message-ID: <89e95372aa5f466c8fd3654cbb47bf3e@BLUPR66MB0051.019D.MGD.MSFT.NET> Content-Type: text/plain; charset="us-ascii" Hi Everyone! I am wondering what antibodies everyone uses to identify mesotheliomas, specifically sarcomatous mesothelioma and if there is a negative antibody you use to confirm. Thanks! Anna Anna C. Hughes, HTL (ASCP), QIHC Senior Scientist, Morphologic Pathology anna.c.hughes@gsk.com ------------------------------ Message: 8 Date: Wed, 18 Mar 2015 20:15:48 +0000 From: "Algeo, Lacie A" Subject: [Histonet] bone marrow billing fees To: "histonet@lists.utsouthwestern.edu" Message-ID: <24C4B3C167E5694887AB594C7602CE3A03BAB8A0@WN35104.or.providence.org> Content-Type: text/plain; charset="us-ascii" Hi Everyone, I was wondering if anyone is billing for a Histo technical charge on bone marrow aspirates and biopsies (i.e. an 88305) in addition to all of the Hematology fee codes. Our Histology department does the grossing, embedding and microtomy for all bone marrow blocks, however we do not bill for/receive any revenue for this work. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. ------------------------------ Message: 9 Date: Wed, 18 Mar 2015 20:17:38 +0000 From: "Abbott, Tanya" Subject: [Histonet] ANP.22760 New Reagent Lot Confirmation of Acceptability To: "histonet@lists.utsouthwestern.edu" Message-ID: <852F7D2C14FB464D80E182B15DB138AF4CDCBAFD@CHIEX005.CHI.catholichealth.net> Content-Type: text/plain; charset="us-ascii" Can you tell it's "review the CAP checklist time"?!! In regards to ANP.22760, New Reagent Lot Confirmation of Acceptability, how does everyone handle it? I have a procedure in place, but I would like it to flow a bit better. Thanks in advance! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ------------------------------ Message: 10 Date: Wed, 18 Mar 2015 16:45:16 -0400 From: "Whitaker, Bonnie" Subject: [Histonet] RE: bone marrow billing fees To: "'Algeo, Lacie A'" , "histonet@lists.utsouthwestern.edu" Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670E14E23113@EXMBOX-VP05.OSUMC.EDU> Content-Type: text/plain; charset=us-ascii Hi Lacie, Anything that is grossed, embedded and has slides cut and stained by histology (core bx or clot) should be billed an 88305. Bonnie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A Sent: Wednesday, March 18, 2015 4:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone marrow billing fees Hi Everyone, I was wondering if anyone is billing for a Histo technical charge on bone marrow aspirates and biopsies (i.e. an 88305) in addition to all of the Hematology fee codes. Our Histology department does the grossing, embedding and microtomy for all bone marrow blocks, however we do not bill for/receive any revenue for this work. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=qJ2DbHovRUIxlF1AnAt9XBNpD_BCv8Upx8EJDnWyG5s&s=dM8BUM3L49UUcAoPk9kwSmVe3om4g-NO9Gw-GKfDGCQ&e= ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 136, Issue 23 ***************************************** ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From tbraud <@t> holyredeemer.com Thu Mar 19 12:47:52 2015 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Mar 19 12:48:18 2015 Subject: [Histonet] RE: Bone marrow Charges In-Reply-To: <20150319170328.3F9EA1EB280@trendmess-svr.holyredeemer.local> References: <20150319170328.3F9EA1EB280@trendmess-svr.holyredeemer.local> Message-ID: In our Histology Lab, we charge: 88305 X2 for Gross and Micro on both clot and core bx 88311 for Decalcification of the bone core bx 88313 X2 for the Iron Stain and Retic Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 8. bone marrow billing fees (Algeo, Lacie A) Message: 8 Date: Wed, 18 Mar 2015 20:15:48 +0000 From: "Algeo, Lacie A" Subject: [Histonet] bone marrow billing fees Hi Everyone, I was wondering if anyone is billing for a Histo technical charge on bone marrow aspirates and biopsies (i.e. an 88305) in addition to all of the Hematology fee codes. Our Histology department does the grossing, embedding and microtomy for all bone marrow blocks, however we do not bill for/receive any revenue for this work. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From wsimons <@t> athensgastro.com Thu Mar 19 13:28:24 2015 From: wsimons <@t> athensgastro.com (wsimons@athensgastro.com) Date: Thu Mar 19 13:28:34 2015 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gRnVuZ3VzIGNvbnRyb2w=?= In-Reply-To: <24C4B3C167E5694887AB594C7602CE3A03BABB11@WN35104.or.providence.org> References: <24C4B3C167E5694887AB594C7602CE3A03BABB11@WN35104.or.providence.org> Message-ID: <20150319182824.23877.qmail@server276.com> I use the same, an orange peel, and a small piece of colon (or other suitable tissue) for contrast. Wanda K. Simons, HT (ASCP) Athens Gastroenterology Association 3320 Old Jefferson Road, Bldg.400 Athens, GA 30607 706.613.1625 www.histosearch.com/gsh/ > -------Original Message------- > From: Algeo, Lacie A > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Fungus control > Sent: Mar 19 '15 13:36 > > We used an orange.??Leave it in a brown bag until it grows fungus.??Then you can cut strips of the peel and process just like your regular tissue.??You get all the hyphae nicely defined in the peel.??The counterstain doesn't really show, though, since it is plant cells. > > > Lacie Algeo, HTL (ASCP) MBCM > Histology Supervisor > Providence Sacred Heart Medical Center Laboratory > 101 W 8th Avenue > L-2 > Spokane, WA 99204 > 509-474-4418 > FAX 509-474-2052 > lacie.algeo@providence.org > > > This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law.??If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message.??If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu > Sent: Thursday, March 19, 2015 10:06 AM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 136, Issue 23 > > Send Histonet mailing list submissions to > ????????histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ????????http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ????????histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > ????????histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." > > > Today's Topics: > > ?? 1. HT Job at Plum Island - JUST 2 DAYS LEFT (Arzt, Jonathan) > ?? 2. Used Tissue Embedding Station (May Wei) > ?? 3. RE: [EXTERNAL] [Histonet] GMS controls? (Norton, Sally) > ?? 4. Dako Omnis (Michael Farmer) > ?? 5. NYSHS 2015 Corporate Educational Scholarships (Mack, Sarah) > ?? 6. RE: Dako Omnis (Susan Foreman) > ?? 7. sarcomatous mesothelioma (Anna Hughes) > ?? 8. bone marrow billing fees (Algeo, Lacie A) > ?? 9. ANP.22760 New Reagent Lot Confirmation of Acceptability > ??????(Abbott, Tanya) > ??10. RE: bone marrow billing fees (Whitaker, Bonnie) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 18 Mar 2015 17:08:19 +0000 > From: "Arzt, Jonathan" > Subject: [Histonet] HT Job at Plum Island - JUST 2 DAYS LEFT > To: "'histonet@lists.utsouthwestern.edu'" > ???????? > Message-ID: > ????????<7213AC15544DE945843D821E1769E38A06A299E2@001FSN2MPN1-052.001f.mgd2.msft.net> > > Content-Type: text/plain; charset="us-ascii" > > > Immediately available and open for application for JUST 2 DAYS, is a full-time, permanent, federal histotchnologist position with the Foreign Animal Disease Research Unit at the Plum Island Animal Disease Center. > > Job Title: Biologist (Histotechnologist) > Department: Department Of Agriculture > Agency: Agricultural Research Service > Job Announcement Number: ARS-S15E-0045 > SALARY RANGE:??$44,617.00 to $85,841.00 / Per Year > > See the full posting and application details here: > > https://www.usajobs.gov/GetJob/ViewDetails/396084800 > > > > > > ------------------------------ > > Message: 2 > Date: Wed, 18 Mar 2015 17:22:11 +0000 > From: May Wei > Subject: [Histonet] Used Tissue Embedding Station > To: "Histonet@lists.utsouthwestern.edu" > ???????? > Message-ID: > ???????? > > Content-Type: text/plain; charset="us-ascii" > > I am looking for used Tissue Embedding Station.??If you know anyone who might offer, please contact me. > > Thank you and regards, > > May > > May Wei, M.Med., MBA > Director, International Sales > Diagnostic BioSystems > 6616 Owens Drive,??Pleasanton, CA 94588, USA > Tel: +1 925-484-3350??Mobile: +1 415-812-8095 Toll: +1 888-896-3350??Fax: +1 925-484-3390 > E-mail: may@dbiosys.com??URL: www.dbiosys.com > > > > > > ------------------------------ > > Message: 3 > Date: Wed, 18 Mar 2015 17:29:07 +0000 > From: "Norton, Sally" > Subject: RE: [EXTERNAL] [Histonet] GMS controls? > To: "'McAnn, Sherrian'" , Paula Sicurello > ????????, HistoNet > Message-ID: > ????????<1357F84B33D39A46BA015A8EC6ABCBD040EB630B@PPWEXD01a.childrens.sea.kids> > > Content-Type: text/plain; charset="utf-8" > > We used Brie that we let grow mold for fungus. > > Sally Norton > Histo Tech > Seattle children's Hospital > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian > Sent: Wednesday, March 18, 2015 9:58 AM > To: Paula Sicurello; HistoNet > Subject: RE: [EXTERNAL] [Histonet] GMS controls? > > I have experimented on some things and found Blue Cheese to be excellent source of not only fungus but yeast and some bacteria.?? I did make a couple of smears with the blue cheese but seemed to do much better after just routine processing some of the crumbles of blue cheese in cassettes. > Sincerely, > Sherrian McAnn > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > Sent: Tuesday, March 17, 2015 5:51 PM > To: HistoNet > Subject: [EXTERNAL] [Histonet] GMS controls? > > Good Afternoon Netters, > > I have a project to validate a special stainer and will be starting with GMS.??What can I use as a good source of fungus???Was it last week there was mention of orange peels and onions? > > I need to get the OK of the attending pathologist, so I probably can't use anything too funky, like fruits and vegetables. > > I don't have access to fresh lung tissue to smear cream cheese on (also mentioned last week) so I was hoping some type of easy to buy processed food (like Slim Jims from Gram stain) is out there to be used as a fungal control. > > Ideas? > > Thanks in advance. > > Paula :-) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > ------------------------------ > > Message: 4 > Date: Wed, 18 Mar 2015 13:31:17 -0400 > From: Michael Farmer > Subject: [Histonet] Dako Omnis > To: Histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=us-ascii > > I would appreciate hearing from anyone who has (or has seriously considered) an Omnis. > > Just a brief sentence or two on how it's going would be nice. > > Much obliged > > Michael Farmer > McEvoy & Farmer Pathology > > michael@mcevoyandfarmer.com > cell 415-994-8852 > > > ------------------------------ > > Message: 5 > Date: Wed, 18 Mar 2015 13:50:02 -0400 > From: "Mack, Sarah" > Subject: [Histonet] NYSHS 2015 Corporate Educational Scholarships > To: "histonet@lists.utsouthwestern.edu" > ???????? > Message-ID: > ???????? > > Content-Type: text/plain; charset="iso-8859-1" > > The Awards Committee, formed from the Officers and Directors of the society, carefully evaluate all nominees and supporting documentation submitted for the scholarships. Recipients are presented their award at the New York State Histotechnological Society annual spring meeting. > > To be eligible, please send an application to the Awards Chairperson specifying the award you are applying for and include the following items: > > You must be a member in good standing (the current year). > > A letter from you showing evidence of your commitment to the profession. > > One letter of recommendation from a supervisor, pathologist, histotechnologist or professor. > > Name and address of your current employer or school, your current mailing address and email address. > > Applications are due March 31st, 2015. > > We prefer email submission of applications and letters of recommendation. Please specify which awards you are applying for in your correspondence. Please send documents to: > > Angela M. Fogg, HT ASCP angelafogg@aol.com > > Mailing address: 5 Hammersley Ave Poughkeepsie, NY 12601 > > 2015 Scholarships > Gulf Coast Instrument Company: Is sponsoring a $250 scholarship. The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The awards must be used to defray educational expenses. > Mercedes Medical Award: The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The $250 award is sponsored by Mercedes Medical and must be used to defray educational expenses. > Leica Biosystems Award: The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The $250 award is sponsored by Leica Biosystems and must be used to defray educational expenses. > Sakura Finetek: The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The $250 award is sponsored by Sakura Finetek USA and must be used to defray educational expenses. > Source Medical Products Award: The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The $250 award is sponsored by Source Medical Products and must be used to defray educational expenses. > StatLab Medical Products Award: The award is presented to a histology student or a histotech who wishes to attend a professional meeting. The $250 award is sponsored by StatLab Medical Products and must be used to defray educational expenses. > 20 people reached > New York State Histotechnological Society Like ? > > > Sarah Mack > University of Rochester Medical Center > Center for Musculoskeletal Research > Histology, Biochemistry, and Molecular Imaging Core > 601 Elmwood Avenue > Box 665 > Rochester, NY 14642 > (585)-273-3901 > > > ------------------------------ > > Message: 6 > Date: Wed, 18 Mar 2015 14:03:24 -0400 > From: "Susan Foreman" > Subject: RE: [Histonet] Dako Omnis > To: "'Michael Farmer'" , > ???????? > Message-ID: <010201d061a5$d3f23b70$7bd6b250$@labpath.com> > Content-Type: text/plain;?????? charset="us-ascii" > > I would also like to hear any information that anyone has gathered. > > Many Thanks, > Susan Foreman > sforeman@labpath.com > 865-584-1933 > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Farmer > Sent: Wednesday, March 18, 2015 1:31 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Dako Omnis > > I would appreciate hearing from anyone who has (or has seriously considered) an Omnis. > > Just a brief sentence or two on how it's going would be nice. > > Much obliged > > Michael Farmer > McEvoy & Farmer Pathology > > michael@mcevoyandfarmer.com > cell 415-994-8852 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 7 > Date: Wed, 18 Mar 2015 19:01:06 +0000 > From: Anna Hughes > Subject: [Histonet] sarcomatous mesothelioma > To: "histonet@lists.utsouthwestern.edu" > ???????? > Message-ID: > ????????<89e95372aa5f466c8fd3654cbb47bf3e@BLUPR66MB0051.019D.MGD.MSFT.NET> > Content-Type: text/plain; charset="us-ascii" > > Hi Everyone! > I am wondering what antibodies everyone uses to identify mesotheliomas, specifically sarcomatous mesothelioma and if there is a negative antibody you use to confirm. > Thanks! > Anna > > Anna C. Hughes, HTL (ASCP), QIHC > Senior Scientist,??Morphologic Pathology anna.c.hughes@gsk.com > > > > ------------------------------ > > Message: 8 > Date: Wed, 18 Mar 2015 20:15:48 +0000 > From: "Algeo, Lacie A" > Subject: [Histonet] bone marrow billing fees > To: "histonet@lists.utsouthwestern.edu" > ???????? > Message-ID: > ????????<24C4B3C167E5694887AB594C7602CE3A03BAB8A0@WN35104.or.providence.org> > Content-Type: text/plain; charset="us-ascii" > > Hi Everyone, > I was wondering if anyone is billing for a Histo technical charge on bone marrow aspirates and biopsies (i.e. an 88305) in addition to all of the Hematology fee codes.??Our Histology department does the grossing, embedding and microtomy for all bone marrow blocks, however we do not bill for/receive any revenue for this work. > Thanks, > Lacie > > Lacie Algeo, HTL (ASCP) MBCM > Histology Supervisor > Providence Sacred Heart Medical Center Laboratory > 101 W 8th Avenue > L-2 > Spokane, WA 99204 > 509-474-4418 > FAX 509-474-2052 > lacie.algeo@providence.org > > > This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law.??If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message.??If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. > > > ________________________________ > > This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. > > > ------------------------------ > > Message: 9 > Date: Wed, 18 Mar 2015 20:17:38 +0000 > From: "Abbott, Tanya" > Subject: [Histonet] ANP.22760 New Reagent Lot Confirmation of > ????????Acceptability > To: "histonet@lists.utsouthwestern.edu" > ???????? > Message-ID: > ????????<852F7D2C14FB464D80E182B15DB138AF4CDCBAFD@CHIEX005.CHI.catholichealth.net> > > Content-Type: text/plain; charset="us-ascii" > > Can you tell it's "review the CAP checklist time"?!! In regards to ANP.22760, New Reagent Lot Confirmation of Acceptability, how does everyone handle it? I have a procedure in place, but I would like it to flow a bit better. > Thanks in advance! Tanya > > Tanya G. Abbott > Manager Technologist > Histology/Cytology > St Joseph Medical Center > (phone) 610-378-2635 > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > > > ------------------------------ > > Message: 10 > Date: Wed, 18 Mar 2015 16:45:16 -0400 > From: "Whitaker, Bonnie" > Subject: [Histonet] RE: bone marrow billing fees > To: "'Algeo, Lacie A'" , > ????????"histonet@lists.utsouthwestern.edu" > ???????? > Message-ID: > ????????<6B106EE8C8AAEF449DEA97921DEC11670E14E23113@EXMBOX-VP05.OSUMC.EDU> > Content-Type: text/plain; charset=us-ascii > > Hi Lacie, > > Anything that is grossed, embedded and has slides cut and stained by histology (core bx or clot) should be billed an 88305. > > Bonnie > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A > Sent: Wednesday, March 18, 2015 4:16 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] bone marrow billing fees > > Hi Everyone, > I was wondering if anyone is billing for a Histo technical charge on bone marrow aspirates and biopsies (i.e. an 88305) in addition to all of the Hematology fee codes.??Our Histology department does the grossing, embedding and microtomy for all bone marrow blocks, however we do not bill for/receive any revenue for this work. > Thanks, > Lacie > > Lacie Algeo, HTL (ASCP) MBCM > Histology Supervisor > Providence Sacred Heart Medical Center Laboratory > 101 W 8th Avenue > L-2 > Spokane, WA 99204 > 509-474-4418 > FAX 509-474-2052 > lacie.algeo@providence.org > > > This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law.??If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message.??If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. > > > ________________________________ > > This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=qJ2DbHovRUIxlF1AnAt9XBNpD_BCv8Upx8EJDnWyG5s&s=dM8BUM3L49UUcAoPk9kwSmVe3om4g-NO9Gw-GKfDGCQ&e= > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 136, Issue 23 > ***************************************** > > ________________________________ > > This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From patpxs <@t> gmail.com Thu Mar 19 15:08:29 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Thu Mar 19 15:08:33 2015 Subject: [Histonet] GMS control Message-ID: Thank you to all who volunteered ideas and control blocks. Of course, the control must be of human origin, so they are digging through their files to find a nice fungusy patient tissue for me. Darn those CAP regulations, they take all the fun out of it. I still want to try an orange or Slim Jim or hotdog. Have a nice day, Paula :-) From jvickroy <@t> SpringfieldClinic.com Thu Mar 19 15:14:28 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Thu Mar 19 15:14:34 2015 Subject: [Histonet] G0461 code for prostate biopsies Message-ID: <9B1A1501A800064397369BD8072E6BCAB3F6A7@E2K10DB.springfieldclinic.com> I am told that in 2015 there are no longer G codes for medicare patient billing of IHC stains. That is great. However the gentleman I talked to also said that now for medicare patients there is only one G code for prostate biopsies. G0461 to be used no matter how many of the biopsies procured. I tried to google this to make sure he was correct. What I found was that non medicare prostate biopsies continue to be billed 88305 but an 88305 is not to be used for a medicare patient with a prostate biopsy. Is this everyone else's understanding? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From jvickroy <@t> SpringfieldClinic.com Thu Mar 19 15:15:34 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Thu Mar 19 15:15:41 2015 Subject: [Histonet] RE: G0461 code for prostate biopsies In-Reply-To: <9B1A1501A800064397369BD8072E6BCAB3F6A7@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCAB3F6A7@E2K10DB.springfieldclinic.com> Message-ID: <9B1A1501A800064397369BD8072E6BCAB3F6CB@E2K10DB.springfieldclinic.com> Apparently the code is G0416 not G0461. Sorry Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com From: Vickroy, James Sent: Thursday, March 19, 2015 3:14 PM To: histonet@lists.utsouthwestern.edu Subject: G0461 code for prostate biopsies I am told that in 2015 there are no longer G codes for medicare patient billing of IHC stains. That is great. However the gentleman I talked to also said that now for medicare patients there is only one G code for prostate biopsies. G0461 to be used no matter how many of the biopsies procured. I tried to google this to make sure he was correct. What I found was that non medicare prostate biopsies continue to be billed 88305 but an 88305 is not to be used for a medicare patient with a prostate biopsy. Is this everyone else's understanding? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From mucram11 <@t> comcast.net Fri Mar 20 10:11:20 2015 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Fri Mar 20 10:11:41 2015 Subject: [Histonet] Sue Paturso TJH In-Reply-To: <1391413059.2787845.1426864230354.JavaMail.zimbra@comcast.net> Message-ID: <1006909044.2788222.1426864280875.JavaMail.zimbra@comcast.net> Could you please contact me?? I have a question and lost the e-mail address. ? Thanks, Pam Marcum From anna.coffey <@t> nih.gov Fri Mar 20 11:35:15 2015 From: anna.coffey <@t> nih.gov (Coffey, Anna (NIH/NCI) [C]) Date: Fri Mar 20 11:36:17 2015 Subject: [Histonet] Fish Skin Histology Message-ID: <5C3E10119A1B824FBE92B08279F74A910178D18A@msgb10.nih.gov> Hello Histonet, I am looking for advice on the best method to prepare fish skin and eyes for sectioning. If you have had experience with this in the past, I'd love to hear what worked for you. Thanks in advance! Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey@nih.gov 301-846-1730 From BZIMMERM <@t> gru.edu Fri Mar 20 12:01:11 2015 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Mar 20 12:01:21 2015 Subject: [Histonet] HISTOPALOOZA GEORGIA SOCIETY FOR HISTOTECHNOLOGY Message-ID: Hello everyone, This meeting is going to be a great one. Beautiful scenic Lake Lanier, near Atlanta, will be the location. This will be a great weekend of relaxation, networking, and getting those CE credits. http://www.histosearch.com/gsh/ Sending everyone a reminder to renew your membership and register for Histopalooza. If you've already done so, please ignore this email. Also, early registration insures waived entrance fees. Your name will be on a list at the gate, otherwise you will be paying $14 daily. This time you can say it's good to be on someone's "list". haha Have a great weekend. Today is the first day of Spring, hallelujah!! Regards, Billie Zimmerman GSH Secretary From PowersK <@t> ccmhonline.com Fri Mar 20 12:09:25 2015 From: PowersK <@t> ccmhonline.com (Powers, Kerry) Date: Fri Mar 20 12:10:42 2015 Subject: [Histonet] refernce lab for diagnosis of Hirschsprung's disease in an adult patient Message-ID: <0014A9A2E551EA479C134402BCAC0F2ACD6ACBA3@LDC-MB-001.ccha.local> We are looking for a reference lab, in the united states, that can help us with the diagnosis of Hirschsprung's disease in an adult patient. Any assistance would be greatly appreciated. Kerry Powers, HTL(ASCP) Histology Supervisor Comanche County Memorial Hospital 3401 W. Gore Blvd Lawton, Ok 73505 P: 580-355-8699 ext 3359 F: 580-585-5462 powersk@ccmhonline.com From tmcampbe <@t> fmh.org Fri Mar 20 13:16:35 2015 From: tmcampbe <@t> fmh.org (Campbell, Tasha M.) Date: Fri Mar 20 13:16:40 2015 Subject: [Histonet] temporary histotech position Message-ID: Hello everyone, I was wondering if there is anyone out there that does temporary work in histo labs in the Frederick MD area. I run a small GI lab and I will be going on maternity leave in October and we would like someone to fill in. I know that there are temp agencies I can go through, but I thought I would try leaving the middle man out first because we are a small lab and are trying to save money and they can have more costs. I would need someone for 8 wks. The tech would have to accession, gross the biopsies and of course process, embed, cut and stain. I also do warthin starry and ABPAS by hand. There are on average 30 blocks a day and 10 specials a day. You do your own thing. Go at your own pace. Flexible time. No one bothers you. Just as long as the work gets done. It's really a walk in the park. If there is anyone in the area that is willing to do this, please email or call me! Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) From wbenton <@t> cua.md Fri Mar 20 13:20:31 2015 From: wbenton <@t> cua.md (Walter Benton) Date: Fri Mar 20 13:20:37 2015 Subject: [Histonet] reference lab for diagnosis of Hirschsprung's disease in an adult patient Message-ID: <0B8979A204680A42B93A52B486088CD9423981C2FB@CUAEXH1.GCU-MD.local> http://www.mayomedicallaboratories.com/test-catalog/search.php?search_type=disease&search=Hirschsprung+Disease https://www.genetests.org/search/tests.php?search=Hirschsprung%20Disease,%20Sequencing%20RET%20Gene&submit=Search&start=0&types%5B%5D=&from=tests https://aravindachakravartilab.org/pro/Genetic_Testing_And_Counseling.html Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Powers, Kerry Sent: Friday, March 20, 2015 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] refernce lab for diagnosis of Hirschsprung's disease in an adult patient We are looking for a reference lab, in the united states, that can help us with the diagnosis of Hirschsprung's disease in an adult patient. Any assistance would be greatly appreciated. Kerry Powers, HTL(ASCP) Histology Supervisor Comanche County Memorial Hospital 3401 W. Gore Blvd Lawton, Ok 73505 P: 580-355-8699 ext 3359 F: 580-585-5462 powersk@ccmhonline.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. From Toni.Rathborne <@t> rwjuh.edu Fri Mar 20 13:21:38 2015 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Fri Mar 20 13:22:20 2015 Subject: [Histonet] reference lab for diagnosis of Hirschsprung's disease in an adult patient In-Reply-To: <0B8979A204680A42B93A52B486088CD9423981C2FB@CUAEXH1.GCU-MD.local> References: <0B8979A204680A42B93A52B486088CD9423981C2FB@CUAEXH1.GCU-MD.local> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F60A82@vap1014.win.rwjuh.edu> http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/80573 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walter Benton Sent: Friday, March 20, 2015 2:21 PM To: Powers, Kerry; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] reference lab for diagnosis of Hirschsprung's disease in an adult patient http://www.mayomedicallaboratories.com/test-catalog/search.php?search_type=disease&search=Hirschsprung+Disease https://www.genetests.org/search/tests.php?search=Hirschsprung%20Disease,%20Sequencing%20RET%20Gene&submit=Search&start=0&types%5B%5D=&from=tests https://aravindachakravartilab.org/pro/Genetic_Testing_And_Counseling.html Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Powers, Kerry Sent: Friday, March 20, 2015 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] refernce lab for diagnosis of Hirschsprung's disease in an adult patient We are looking for a reference lab, in the united states, that can help us with the diagnosis of Hirschsprung's disease in an adult patient. Any assistance would be greatly appreciated. Kerry Powers, HTL(ASCP) Histology Supervisor Comanche County Memorial Hospital 3401 W. Gore Blvd Lawton, Ok 73505 P: 580-355-8699 ext 3359 F: 580-585-5462 powersk@ccmhonline.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Fri Mar 20 14:25:19 2015 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Fri Mar 20 14:25:27 2015 Subject: [Histonet] DPC4/SMAD4 on Ventana platform Message-ID: <77F52EFAB8B1694B885E277C48FCD0F69C823957@GHSEXMBX1W8K1V.geisinger.edu> Has anyone had any luck optimizing DPC4 aka SMAD4 with Ventana's XT or BenchmarkULtra platform? Any help is appreciated. Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From mills <@t> 3scan.com Fri Mar 20 14:30:17 2015 From: mills <@t> 3scan.com (Caroline Miller) Date: Fri Mar 20 14:30:42 2015 Subject: [Histonet] Block staining with HE Message-ID: Hi Histonet, Happy Friday! I was wondering if anyone had access to this paper: Stain Technol. 1981 Mar;56(2):119-23. Block staining of mammalian tissues with hematoxylin and eosin. or if anyone had some tips on whole-mount HE staining I would gladly take it! I am just starting on this quest, but so far I have tried vacuum for the haematoxylin overnight with Erlichs and Harris's, and I have some Mayers on order. The Erlichs and harris's really overstained the tissue and I have been destaining for a while. I am thinking Mayer's may be better for its progressive nature, but any advice will be gratefully taken! Thank you in advance for your fantastic advice! yours, Caroline -- Caroline Miller Director of Histology 3Scan.com 415 2187297 From cbrya <@t> lexclin.com Fri Mar 20 15:19:16 2015 From: cbrya <@t> lexclin.com (Carol Bryant) Date: Fri Mar 20 15:19:22 2015 Subject: [Histonet] scale for weighting parathyroid glands Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF4C422878B0@EXCHANGESB> Hello All, Our surgeons are doing a lot of parathyroid glands for patients with adenomas. Our pathologist would like to weigh them. Our scale is not that sensitive. Is anyone using a scale for this too and if so what brand? Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cbrya@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. From jkiernan <@t> uwo.ca Fri Mar 20 17:13:28 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri Mar 20 17:13:33 2015 Subject: [Histonet] Block staining with HE In-Reply-To: <73c0d491dc11.550c9b6d@uwo.ca> References: <7210e547ab1b.550c8e97@uwo.ca> <71e0c168e3ed.550c8fc4@uwo.ca> <73c08d7fe93b.550c9002@uwo.ca> <73c0bc1b8244.550c9040@uwo.ca> <72e0ced1da25.550c9697@uwo.ca> <7200f80387f1.550c96d6@uwo.ca> <7330d7749340.550c9714@uwo.ca> <71e09159eb58.550c9753@uwo.ca> <7200a9b89eaf.550c97cf@uwo.ca> <7330dd4ba92b.550c9849@uwo.ca> <73c0abd2cea0.550c98c4@uwo.ca> <73c0d0ababf0.550c9902@uwo.ca> <7310a74ece31.550c9940@uwo.ca> <71e0c298d90b.550c997e@uwo.ca> <7200bc10a306.550c99f9@uwo.ca> <7390f897e53a.550c9a37@uwo.ca> <72109a07d62a.550c9a75@uwo.ca> <7360c9caa1d1.550c9ab3@uwo.ca> <73c0e6a1dd98.550c9af1@uwo.ca> <73c0c670d007.550c9b2f@uwo.ca> <73c0d491dc11.550c9b6d@uwo.ca> Message-ID: <7310b908b805.550c5538@uwo.ca> You need to read the paper. It says "All routinely used hematoxylins, such as Ehrlich?s, Mayer?s, Harris? and Delafield?s, proved unsatisfactory" STAIN TECHNOLOGY Vol. 56, No. 2, pp. 119-123 (1981) BLOCK STAINING OF MAMMALIAN TISSUES WITH HEMATOXYLIN AND EOSIN IAN F. HINE, Department of Anatomy, Monash University, Clayton, Victoria 3168, Australia. ABSTRACT. Various mammalian tissues were stained en bloc with hematoxylin and eosin after fixation and prior to embedding in paraffin wax and sectioning. The choice of fixative is important and best results are obtained using Worcester?s Fluid, a combination of saturated aqueous mercuric chloride, formaldehyde, and glacial acetic acid. After fixation, blocks of tissue up to 1.5 em thick are stained for seven days in hematoxylin. Excess stain is removed by washing tissues in running water overnight. Tissue blocks then are dehydrated with graded concentrations of ethyl alcohols to 80% and counterstained, with further dehydration, in 0.5% spirit soluble eosin in 90% ethyl alcohol for five days. The tissue is subsequently transferred to 90% ethyl alcohol overnight to differentiate win staining; dehydration is completed in absolute ethyl alcohol. The blocks are cleared in cedarwood oil and briefly in xylene prior to embedding, sectioning, and mounting. Following removal of wax by xylene, coverslips are applied. General morphological and histological features were particularly well differentiated and very selectively and reliably stained by this method. Hine's "Worcester's" fixative is similar to Stieve's. His haemalum formulation is a little unusual. The counterstain was ethyl eosin (CI 45385), not eosin Y (CI 45380). There is a page of colour photos in which the red/blue colour separation is satisfactory in only two (my opinion). One photo shows a block-stained H&E section subsequently stained with Masson's and Verhoeff's, with bluish green collagen and black elastic laminae. John Kiernan London, Canada = = = On 20/03/15, Caroline Miller wrote: > Hi Histonet, Happy Friday! > > I was wondering if anyone had access to this paper: > Stain Technol. > > 1981 Mar;56(2):119-23. > Block staining of mammalian tissues with hematoxylin and eosin. > > or if anyone had some tips on whole-mount HE staining I would gladly take > it! > > I am just starting on this quest, but so far I have tried vacuum for the > haematoxylin overnight with Erlichs and Harris's, and I have some Mayers on > order. The Erlichs and harris's really overstained the tissue and I have > been destaining for a while. I am thinking Mayer's may be better for its > progressive nature, but any advice will be gratefully taken! > > Thank you in advance for your fantastic advice! > > yours, > > Caroline > > > > > -- > Caroline Miller > Director of Histology > 3Scan.com > 415 2187297 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rmire <@t> cvpath.org Sat Mar 21 09:24:29 2015 From: rmire <@t> cvpath.org (Ronda Mire) Date: Sat Mar 21 09:24:39 2015 Subject: [Histonet] temporary histotech position In-Reply-To: References: Message-ID: <50B4464E-F8EA-4140-8E00-BABA59B32224@cvpath.org> Hi Tasha, I live in Frederick, MD and would be happy to fill in for you. I am board registered HT with 20 years experience. I worked at Meritus Medical Lab for 22 years and then at CVPath Institute. I would be happy to meet with you or send my resume for you review. Kind regards, Ronda Mire 240-217-9881 > On Mar 20, 2015, at 2:16 PM, Campbell, Tasha M. wrote: > > Hello everyone, I was wondering if there is anyone out there that does temporary work in histo labs in the Frederick MD area. I run a small GI lab and I will be going on maternity leave in October and we would like someone to fill in. I know that there are temp agencies I can go through, but I thought I would try leaving the middle man out first because we are a small lab and are trying to save money and they can have more costs. I would need someone for 8 wks. The tech would have to accession, gross the biopsies and of course process, embed, cut and stain. I also do warthin starry and ABPAS by hand. There are on average 30 blocks a day and 10 specials a day. You do your own thing. Go at your own pace. Flexible time. No one bothers you. Just as long as the work gets done. It's really a walk in the park. > > If there is anyone in the area that is willing to do this, please email or call me! > > Thanks!! > > > > > > Tasha Campbell, B.S.,HTL(ASCP) > Frederick Gastroenterology Associates > 310 W. 9th St. > Frederick, MD 21701 > 301-695-6800 ext. 144 (w) > 304-685-9307 (c) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann <@t> aol.com Sat Mar 21 12:14:25 2015 From: thisisann <@t> aol.com (Ann Specian) Date: Sat Mar 21 12:14:29 2015 Subject: [Histonet] Post fixing IHC slides in formalin Message-ID: <14c3d541eb9-2d17-14f84@webprd-m104.mail.aol.com> Does anyone post fix their IHC slides in formalin in an effort to try to reduce tissue loss? If so, does anyone have a protocol for this that they have used and have seen good results? If you have any other suggestions which can help to reduce tissue loss during IHC staining, I would love to hear from you. Thanks, Ann From barryrittman <@t> gmail.com Sat Mar 21 13:20:02 2015 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Sat Mar 21 13:20:05 2015 Subject: [Histonet] Post fixing IHC slides in formalin In-Reply-To: <14c3d541eb9-2d17-14f84@webprd-m104.mail.aol.com> References: <14c3d541eb9-2d17-14f84@webprd-m104.mail.aol.com> Message-ID: There are always discussions about fixation but I have never seen comments about using vapor fixation for post fixing or for fixing fresh frozen specimens. Vapor fixation is simple to use and does not require any solvent as it uses the tissue fluids in the case of fresh frozen sections or the solution remaining after IHC. Especially useful for formaldehyde, alcohol, acetone, osmium tetroxide etc. Barry On Sat, Mar 21, 2015 at 12:14 PM, Ann Specian wrote: > Does anyone post fix their IHC slides in formalin in an effort to try to > reduce tissue loss? If so, does anyone have a protocol for this that they > have used and have seen good results? > > > If you have any other suggestions which can help to reduce tissue loss > during IHC staining, I would love to hear from you. > Thanks, > Ann > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rsrichmond <@t> gmail.com Sun Mar 22 16:36:17 2015 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sun Mar 22 16:36:21 2015 Subject: [Histonet] Re: scale for weighing parathyroid glands Message-ID: Carol Bryant, CT (ASCP), Cytology/Histology Manager at the Lexington [Kentucky] Clinic asks? >>Our surgeons are doing a lot of parathyroid glands for patients with adenomas. Our pathologist would like to weigh them. Our scale is not that sensitive. Is anyone using a scale for this too and if so what brand?<< You need a top-loading balance that will weigh in 10 milligram (.01 g) increments, up to about 200 grams. Ohaus makes these for Fisher, and there are other brands. About $500. Weighing parathyroid adenomas is standard of care, as your pathologist knows. Persuading the lab manager or assistant administrator is a different problem. Bob Richmond Samurai Pathologist Maryville TN From d.a.faichney <@t> stir.ac.uk Mon Mar 23 04:41:41 2015 From: d.a.faichney <@t> stir.ac.uk (Debbie Faichney) Date: Mon Mar 23 04:42:03 2015 Subject: [Histonet] RE: Fish Skin Histology In-Reply-To: <5C3E10119A1B824FBE92B08279F74A910178D18A@msgb10.nih.gov> References: <5C3E10119A1B824FBE92B08279F74A910178D18A@msgb10.nih.gov> Message-ID: <8ED3F2CA5B78E142B8193376C57330F801168BD926D1@EXCH2007.ad.stir.ac.uk> Hi Anna, Working in Aquaculture, we section fish tissues daily - typically Salmonids. What works for us is routinely process to paraffin, then surface/trim the block followed by surface decal (for at least an hour) using a hydrochloric acid based product (RDC, Cellpath UK.) Whole eyes do not process well unless very small, therefore we lay the eye cornea side down and cut out a transverse section on either side of the optic nerve prior to processing - hope this makes sense! It can help sectioning if the skin is positioned in the block perpendicular, rather than horizontal, to the blade. Prior to sectioning, the blocks are chilled for a few minutes- skin is placed face down on the cold plate eyes are placed face-up on the cold plate with a drop of distilled water on the lens to aid sectioning. What species of fish are you working with? Some cause more problems than others. Hope this helps! Debbie Debbie Faichney, BSc Senior Technician Histology/Bacteriology Laboratories Institute of Aquaculture University of Stirling Stirling, FK9 4LA Scotland UK Tel: +44(0)1786 466592/466590 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Coffey, Anna (NIH/NCI) [C] Sent: 20 March 2015 16:35 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fish Skin Histology Hello Histonet, I am looking for advice on the best method to prepare fish skin and eyes for sectioning. If you have had experience with this in the past, I'd love to hear what worked for you. Thanks in advance! Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey@nih.gov 301-846-1730 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The University of Stirling has been ranked in the top 12 of UK universities for graduate employment*. 94% of our 2012 graduates were in work and/or further study within six months of graduation. *The Telegraph The University of Stirling is a charity registered in Scotland, number SC 011159. From Valerie.Hannen <@t> parrishmed.com Mon Mar 23 04:57:25 2015 From: Valerie.Hannen <@t> parrishmed.com (Hannen, Valerie) Date: Mon Mar 23 04:59:02 2015 Subject: [Histonet] Re: scale for weighing parathyroid glands In-Reply-To: References: Message-ID: <450B7A81EDA0C54E97C53D60F00776C32337384232@isexstore03> We use the Ohaus scale. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen@parrishmed.com www.parrishmed.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Sunday, March 22, 2015 5:36 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: scale for weighing parathyroid glands Carol Bryant, CT (ASCP), Cytology/Histology Manager at the Lexington [Kentucky] Clinic asks? >>Our surgeons are doing a lot of parathyroid glands for patients with adenomas. Our pathologist would like to weigh them. Our scale is not that sensitive. Is anyone using a scale for this too and if so what brand?<< You need a top-loading balance that will weigh in 10 milligram (.01 g) increments, up to about 200 grams. Ohaus makes these for Fisher, and there are other brands. About $500. Weighing parathyroid adenomas is standard of care, as your pathologist knows. Persuading the lab manager or assistant administrator is a different problem. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From jshelley <@t> sanfordburnham.org Mon Mar 23 08:53:39 2015 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Mon Mar 23 08:53:51 2015 Subject: [Histonet] Florida Society for Histotechnology 2015 Spring Meeting Message-ID: Good Morning! I wanted to let everyone know that the FSH 2015 Meeting Program is complete and we are looking forward to a great meeting on May 14-17, 2015 at the Lake Buena Vista Palace Hotel and Spa. We especially invite those here in the State of Florida to come in order to fulfill your CEU requirements for both your state licensure and for you HT/HTL certification. We are also extending the invitation to those of you outside the state if you are looking to get away from the winter and would like the wonderful bright sun and vacation atmosphere of our location. Our meeting will be nestled within the Disney area and would be a great opportunity for learning and relaxation. I have included some key links to be able to make your plans that much easier. I look forward to seeing you at the meeting. Meeting Program/agenda http://www.fshgroup.org/wp-content/uploads/2015/03/FSH-2015-Online-Program-revised-6.pdf Hotel online reservation https://reservations.ihotelier.com/crs/g_reservation.cfm?groupID=1251402&hotelID=6579 Online meeting registration https://www.regonline.com/Register/Checkin.aspx?EventID=1679155&lbrd=1&rtypeid=380141 Sincerely, John Shelley 2014-16 FSH President From cjohnson <@t> nmda.nmsu.edu Mon Mar 23 11:22:17 2015 From: cjohnson <@t> nmda.nmsu.edu (Johnson, Carole) Date: Mon Mar 23 11:22:24 2015 Subject: [Histonet] Charge for unstained slides? Message-ID: Good morning all, Would anyone mind sharing what their lab charges for generating unstained slides? I have a researcher who has requested 40-some, and I want to make sure I'm being reasonable but not cheap. Thanks in advance! Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From blayjorge <@t> gmail.com Mon Mar 23 12:05:09 2015 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Mon Mar 23 12:05:17 2015 Subject: [Histonet] TEM: Sensory vs. glandular structures on insect wings Message-ID: Dear Histonet-Listers: I am involved in transmission electron microscopy (TEM) study on insect wing organs. Beside veins, in cross section I have seen other structures. How could I tell whether those structures are sensory or glandular? I have searched the web for images and I am getting a sense of what to look but yet have to come across images contrasting, in plain language, how would a "typical" sensory structure on the insect wing would look (in cross section with TEM) vs. a glandular structure (again, in cross section with TEM). If you know of resources I could tap, please kindly contact me off line (email below). With gratefulness, Jorge blayjorge@gmail.com Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From tmcampbe <@t> fmh.org Mon Mar 23 12:08:10 2015 From: tmcampbe <@t> fmh.org (Campbell, Tasha M.) Date: Mon Mar 23 12:08:31 2015 Subject: [Histonet] staining racks Message-ID: Does anyone have any Leica staining racks for the autostainer XL that they were sell/donate to me?? I just got a quote and its over $300 for 1 rack!!! That is crazy!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) From kiran_g <@t> sbcglobal.net Mon Mar 23 12:15:14 2015 From: kiran_g <@t> sbcglobal.net (Kiranjit Grewal) Date: Mon Mar 23 12:18:11 2015 Subject: [Histonet] Open Position : Histology/IHC Manager Message-ID: <1427130914.55383.YahooMailBasic@web184802.mail.gq1.yahoo.com> Dear Histology Community, Excellent opportunity to work with Kaiser Permanente NCAL Regional Lab. Berkeley, CA. Minimum Education: Bachelor degree Experience: 3-5 year as Supervisor/Manager in high volume lab. ASCP Certification: QIHC (Strong technical background in IHC preferred), HT cerfication is not required. Hours of the position are 8am-5 PM Monday-Friday. If you're interested, please contact me. Thank you, Kiran Kiranjit Grewal, MS, CLS, HTL/QIHC(ASCP) Director of Lab. Services Anatomic Pathology. Kaiser Regional Lab. Berkeley, CA ? Kiranjit.K.Grewal@kp.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Mon Mar 23 14:11:03 2015 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Mon Mar 23 14:11:11 2015 Subject: [Histonet] Need paraffin control block for C4d on kidney tissue In-Reply-To: References: Message-ID: Our current kidney paraffin tissue control that we use for C4d by IHC, as well as the full Ig panel on ffpe kidneys, is getting thin and we are having some difficulty finding a suitable control. Does anyone has a block they would be willing to send us? I may be able to trade for another control block. Thanks in advance for your help. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? From JMacDonald <@t> mtsac.edu Mon Mar 23 21:51:57 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Mar 23 22:01:39 2015 Subject: [Histonet] BS in Histotechnology Message-ID: In what areas would a facility hire an HTL over an HT? Is there a need for more HTL programs? 4 Thank you, From akbitting <@t> geisinger.edu Tue Mar 24 09:34:03 2015 From: akbitting <@t> geisinger.edu (Bitting, Angela K.) Date: Tue Mar 24 09:34:11 2015 Subject: [Histonet] lab dishwasher Message-ID: <77F52EFAB8B1694B885E277C48FCD0F69C826CBD@GHSEXMBX1W8K1V.geisinger.edu> Can anyone recommend a good dishwasher for cleaning my special stains glassware? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From rjbuesa <@t> yahoo.com Tue Mar 24 10:31:21 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 24 10:31:26 2015 Subject: [Histonet] lab dishwasher In-Reply-To: <77F52EFAB8B1694B885E277C48FCD0F69C826CBD@GHSEXMBX1W8K1V.geisinger.edu> References: <77F52EFAB8B1694B885E277C48FCD0F69C826CBD@GHSEXMBX1W8K1V.geisinger.edu> Message-ID: <735777477.483078.1427211081268.JavaMail.yahoo@mail.yahoo.com> Nothing will give you the results you will obtain by hand cleaning you SS glassware!??Ren? J. On Tuesday, March 24, 2015 10:35 AM, "Bitting, Angela K." wrote: Can anyone recommend a good dishwasher for cleaning my special stains glassware? IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Tue Mar 24 10:47:05 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Tue Mar 24 10:48:43 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> Jennifer, we require a BA/BS degree for all Histotechnologist positions. However, in our 4 step categories Level 1 does not require certification, just the degree and the requirement that they get the certification within a year. Advancement to level 2 to 4 requires an HT or HTL certification (Level 1 = entry level bench tech, Level 2 is bench tech, level 3 is senior tech, level 4 is Lead tech). Supervisor requires and HTL. Considering that we already require a BA/BS degree for all levels, the fact a person has a HT or HTL is not going to matter much for levels 1 thru 4, only for supervisor level. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, March 23, 2015 7:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BS in Histotechnology In what areas would a facility hire an HTL over an HT? Is there a need for more HTL programs? 4 Thank you, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Tue Mar 24 11:06:13 2015 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Mar 24 11:06:23 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> Message-ID: <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> So just out of curiosity is the pay on the same level as that of a Med Tech with a BS? Does the BA/BS have to be in Histotechnology or is the BA/BS followed by one of the on-line certificate programs? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, March 24, 2015 11:47 AM To: Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Jennifer, we require a BA/BS degree for all Histotechnologist positions. However, in our 4 step categories Level 1 does not require certification, just the degree and the requirement that they get the certification within a year. Advancement to level 2 to 4 requires an HT or HTL certification (Level 1 = entry level bench tech, Level 2 is bench tech, level 3 is senior tech, level 4 is Lead tech). Supervisor requires and HTL. Considering that we already require a BA/BS degree for all levels, the fact a person has a HT or HTL is not going to matter much for levels 1 thru 4, only for supervisor level. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, March 23, 2015 7:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BS in Histotechnology In what areas would a facility hire an HTL over an HT? Is there a need for more HTL programs? 4 Thank you, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From PAMarcum <@t> uams.edu Tue Mar 24 11:16:12 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Mar 24 11:16:18 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> Message-ID: <2d538e6fc6fc490eb6522a4929335f6b@MAIL13M2N2.ad.uams.edu> In most cases it means nothing and if you are in Histology and the administration considers Histology a non-professional laboratory personnel area the pay is lower. Sorry I have fought this for 5.5 years here and the difference between an HT and HTL is the degree only not the registry. If it is $0.10 an hour it is good. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Tuesday, March 24, 2015 11:06 AM To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology So just out of curiosity is the pay on the same level as that of a Med Tech with a BS? Does the BA/BS have to be in Histotechnology or is the BA/BS followed by one of the on-line certificate programs? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, March 24, 2015 11:47 AM To: Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Jennifer, we require a BA/BS degree for all Histotechnologist positions. However, in our 4 step categories Level 1 does not require certification, just the degree and the requirement that they get the certification within a year. Advancement to level 2 to 4 requires an HT or HTL certification (Level 1 = entry level bench tech, Level 2 is bench tech, level 3 is senior tech, level 4 is Lead tech). Supervisor requires and HTL. Considering that we already require a BA/BS degree for all levels, the fact a person has a HT or HTL is not going to matter much for levels 1 thru 4, only for supervisor level. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, March 23, 2015 7:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BS in Histotechnology In what areas would a facility hire an HTL over an HT? Is there a need for more HTL programs? 4 Thank you, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Timothy.Morken <@t> ucsf.edu Tue Mar 24 11:42:04 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Tue Mar 24 11:42:10 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> Message-ID: <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> Tom, no, Histo does start lower than med techs, but consider that a med tech has specialty training from the time they decide to go that route while most histotechs have general biology degrees and nothing but on the job training. Even with a certification a Histotech is not at the same level as a med tech simply due to the unstructured nature of their self-education and training. In 30+ years I have met only a handful of people who got any sort of degrees in Histotechnology, so waiting for those people to come along is not going to work for hiring. Most of our staff got their certification while working here and did it on their own. Only one has a degree in Histotechnology, and a BS at that!. A starting salary here is $36/hr and it is a $3 to $4 increase per level. The lab staff is unionized, and we compete with many large service labs (ie Kaiser) and many, many large biotech companies for the same pool of techs. Plus, it is expensive to live in the San Francisco Bay Area. We only recently (a few years ago) started this requirement in order to get our staff to a higher level. We still have staff without BA/BS degrees. The degree just needs to meet the requirements for certification so does not need to be a specialty degree. Tim -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Tuesday, March 24, 2015 9:06 AM To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology So just out of curiosity is the pay on the same level as that of a Med Tech with a BS? Does the BA/BS have to be in Histotechnology or is the BA/BS followed by one of the on-line certificate programs? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, March 24, 2015 11:47 AM To: Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Jennifer, we require a BA/BS degree for all Histotechnologist positions. However, in our 4 step categories Level 1 does not require certification, just the degree and the requirement that they get the certification within a year. Advancement to level 2 to 4 requires an HT or HTL certification (Level 1 = entry level bench tech, Level 2 is bench tech, level 3 is senior tech, level 4 is Lead tech). Supervisor requires and HTL. Considering that we already require a BA/BS degree for all levels, the fact a person has a HT or HTL is not going to matter much for levels 1 thru 4, only for supervisor level. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, March 23, 2015 7:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BS in Histotechnology In what areas would a facility hire an HTL over an HT? Is there a need for more HTL programs? 4 Thank you, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From suetp918 <@t> comcast.net Tue Mar 24 11:59:20 2015 From: suetp918 <@t> comcast.net (Sue) Date: Tue Mar 24 11:59:37 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> Message-ID: <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> This is a fight that we continue to have with hospital administration.? In my opinion histologists are just as important and needed as MT.? Even though there is an increase in automation in pathology the hands on of a histologists is most important.? The fact that hospital still consider a lower entry job is the reason there are not more of us.? It is quite frustrating. ? Sue TJUH From mucram11 <@t> comcast.net Tue Mar 24 12:12:31 2015 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Tue Mar 24 12:12:47 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> Message-ID: <384355666.3717642.1427217151604.JavaMail.zimbra@comcast.net> It is the only truth I deal with here.? We are, like TJH, University medical school and they only care about the degree, four year is best.? They (administration and/or the pathologists) have never attempted to learn what we have or how we do it?and I doubt they will ever want to learn about Histology.? ? When I started many years ago the residents had to come through Histology for two to six weeks depending on the site.? Now we get 10 minutes to explain what they need to do to get good, not even great slides and stains. They simply are not interested and these will be the people future Histologists have to work for and depend on for pay.? We are in trouble and it is getting deeper.? I have the same question I have had for years: Where is NSH and how are they helping us move forward?? I have seen no movement to help get us raised to Laboratory Professionals.??I have only heard as long as we don't have degrees for our training we will not be recoginzied.? I have the degrees and still have to fight for salary and my rasies while if I were an MT it would be a given.? ? Sorry this is a sore subject and I fight yearly to get bare minimum raises for our people.? We did not get raises at all for two years and that was throughout the labs and hosptial.? Two percent raises are very close to an insult for us.? (We are talking angstrom close; not inches.) ? Pam ----- Original Message ----- From: "Sue" To: "Timothy Morken" Cc: "Histonet" , "Jennifer MacDonald" Sent: Tuesday, March 24, 2015 11:59:20 AM Subject: Re: [Histonet] BS in Histotechnology This is a fight that we continue to have with hospital administration.? In my opinion histologists are just as important and needed as MT.? Even though there is an increase in automation in pathology the hands on of a histologists is most important.? The fact that hospital still consider a lower entry job is the reason there are not more of us.? It is quite frustrating. ? Sue TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From catherinesimonson <@t> gmail.com Tue Mar 24 12:18:20 2015 From: catherinesimonson <@t> gmail.com (Catherine Simonson) Date: Tue Mar 24 12:18:23 2015 Subject: [Histonet] RE: Leica Staining Racks Message-ID: Tasha, That is a little nuts! We have the same stainer, just ordered some replacement racks ourselves. We only paid about $50 per rack. Catherine Simonson, B.S., HT (ASCP) From jqb7 <@t> cdc.gov Tue Mar 24 12:29:31 2015 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue Mar 24 12:29:37 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> Message-ID: <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can teach any trained dog how to section histology will never have the recognition those of us that have studied and trained deserve. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 24, 2015 12:59 PM To: Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: Re: [Histonet] BS in Histotechnology This is a fight that we continue to have with hospital administration.? In my opinion histologists are just as important and needed as MT.? Even though there is an increase in automation in pathology the hands on of a histologists is most important.? The fact that hospital still consider a lower entry job is the reason there are not more of us.? It is quite frustrating. ? Sue TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Tue Mar 24 12:52:37 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Mar 24 12:52:45 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> Message-ID: <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> That was nicer than the pathologist who told me years ago, "any monkey could be trained to do my job". I basically did not take the job I was interviewing for at the time. At least the next interview went a lot better and I did take the job. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 24, 2015 12:30 PM To: Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can teach any trained dog how to section histology will never have the recognition those of us that have studied and trained deserve. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 24, 2015 12:59 PM To: Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: Re: [Histonet] BS in Histotechnology This is a fight that we continue to have with hospital administration.? In my opinion histologists are just as important and needed as MT.? Even though there is an increase in automation in pathology the hands on of a histologists is most important.? The fact that hospital still consider a lower entry job is the reason there are not more of us.? It is quite frustrating. ? Sue TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From b-frederick <@t> northwestern.edu Tue Mar 24 13:03:49 2015 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Mar 24 13:03:57 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> Message-ID: <767de729b36b48c08a0774034c4a982e@evcspmbx03.ads.northwestern.edu> My degree is actually a bachelor of general science with an emphasis in Histotechnology. The only reason I don't have a BS in biology is once I heard I did not need Quant to be a histotech, I rejoiced and did not take it,which would have given me the BS in Biology. As it stands, I say I have a degree in histo. I agree with all in that PI's think anyone can cut (no theory) and NU does not restrict who can but equipment or use it, despite the fact we are the core facility. We are not unionized so I envy your 36/hr. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, March 24, 2015 11:42 AM To: Podawiltz, Thomas; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Tom, no, Histo does start lower than med techs, but consider that a med tech has specialty training from the time they decide to go that route while most histotechs have general biology degrees and nothing but on the job training. Even with a certification a Histotech is not at the same level as a med tech simply due to the unstructured nature of their self-education and training. In 30+ years I have met only a handful of people who got any sort of degrees in Histotechnology, so waiting for those people to come along is not going to work for hiring. Most of our staff got their certification while working here and did it on their own. Only one has a degree in Histotechnology, and a BS at that!. A starting salary here is $36/hr and it is a $3 to $4 increase per level. The lab staff is unionized, and we compete with many large service labs (ie Kaiser) and many, many large biotech companies for the same pool of techs. Plus, it is expensive to live in the San Francisco Bay Area. We only recently (a few years ago) started this requirement in order to get our staff to a higher level. We still have staff without BA/BS degrees. The degree just needs to meet the requirements for certification so does not need to be a specialty degree. Tim -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Tuesday, March 24, 2015 9:06 AM To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology So just out of curiosity is the pay on the same level as that of a Med Tech with a BS? Does the BA/BS have to be in Histotechnology or is the BA/BS followed by one of the on-line certificate programs? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, March 24, 2015 11:47 AM To: Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Jennifer, we require a BA/BS degree for all Histotechnologist positions. However, in our 4 step categories Level 1 does not require certification, just the degree and the requirement that they get the certification within a year. Advancement to level 2 to 4 requires an HT or HTL certification (Level 1 = entry level bench tech, Level 2 is bench tech, level 3 is senior tech, level 4 is Lead tech). Supervisor requires and HTL. Considering that we already require a BA/BS degree for all levels, the fact a person has a HT or HTL is not going to matter much for levels 1 thru 4, only for supervisor level. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, March 23, 2015 7:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BS in Histotechnology In what areas would a facility hire an HTL over an HT? Is there a need for more HTL programs? 4 Thank you, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cjohnson <@t> nmda.nmsu.edu Tue Mar 24 13:10:50 2015 From: cjohnson <@t> nmda.nmsu.edu (Johnson, Carole) Date: Tue Mar 24 13:10:56 2015 Subject: [Histonet] BS in Histology Message-ID: Wow. That was kind of harsh, Tim. I came out of an HT program that required the same prereqs as most med tech programs, so I had that as well as education specific to the field of Histotechnology. My education was VERY structured. I am, however, finishing my BS in Biology so that I can get my HTL credential as well. Oddly enough, I have a good percentage of my BS completed due to the prereqs of my HT program. I realize that there are some programs out there that don't have as stringent requirements, but please don't lump all HT-credentialed professionals into the same group. Those who pass the credentialing exams have demonstrated a body of knowledge that deserves to be recognized and respected. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From lblazek <@t> digestivespecialists.com Tue Mar 24 13:14:52 2015 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Mar 24 13:14:57 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E391737B63B36@IBMB7Exchange.digestivespecialists.com> Fortunately times have changed. The person I replaced in the late 70's early 80's had been brought in from the hospital laundry and was "trained". They were still pouring embedding molds then. She did a beautiful job at cutting and staining H&E slides and 2 or 3 specials but that was all there was to do. This world has come a lot farther than those days of the 80's and 70's. We have grown into fully capable labs that don't have to send work out to the big reference centers to have tests done. I remember a class with Lee Luna when he said "We have to excel and learn these immuno procedures or the MT's were going to take them away from us." We learned and they didn't! We may not be recognized at the level with MT's but we are slowly changing and getting there. At least now an associates is required and CEU's are required. I think that's progress. If you're looked down on in your present position move on! It's not worth it. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 24, 2015 1:53 PM To: 'Sanders, Jeanine (CDC/OID/NCEZID)'; Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology That was nicer than the pathologist who told me years ago, "any monkey could be trained to do my job". I basically did not take the job I was interviewing for at the time. At least the next interview went a lot better and I did take the job. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 24, 2015 12:30 PM To: Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can teach any trained dog how to section histology will never have the recognition those of us that have studied and trained deserve. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 24, 2015 12:59 PM To: Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: Re: [Histonet] BS in Histotechnology This is a fight that we continue to have with hospital administration.? In my opinion histologists are just as important and needed as MT.? Even though there is an increase in automation in pathology the hands on of a histologists is most important.? The fact that hospital still consider a lower entry job is the reason there are not more of us.? It is quite frustrating. ? Sue TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Timothy.Morken <@t> ucsf.edu Tue Mar 24 13:35:30 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Tue Mar 24 13:35:41 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <384355666.3717642.1427217151604.JavaMail.zimbra@comcast.net> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <384355666.3717642.1427217151604.JavaMail.zimbra@comcast.net> Message-ID: <761E2B5697F795489C8710BCC72141FF367FF220@ex07.net.ucsf.edu> Pam, true enough. Indeed, for the annual NSH survey my only comment was that NSH has been ineffective in convincing pathology departments of the value of an HT or HTL certification - to the point that many are now questioning its value at all. Ours is one of few institutions that requires certification for advancement and our medical directors have been pushing for higher quality staff in order to raise the quality of our lab. We went through a pay revision about 7 years ago because the biotech companies and other large medical institutions were sucking up any candidates that poked their head up. We are now on par but still have to fight for any good candidates. But as long as histotechs are on the job trained (probably 99.9% now, as in the past), and invisible to high school and college students, the pay is going nowhere. It is still quite possible to get into the field with no experience . One of our techs got into histology by answering a Craig's list ad placed by a slide mill. He is very good tech, and has a degree in cellular and molecular biology, but that just goes to show how random our source pool is. His education is good, his histology training is random. And another quality candidate just randomly poked his head in my office a few months ago saying he had been doing some histology work in a research lab and was really excited to find out it could be a full time permanent job. We hired him but he is starting in accessioning and will work his way into histology. This is how most people get into histology. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center Tim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Tuesday, March 24, 2015 10:13 AM To: Sue Cc: Histonet; Morken, Timothy; Jennifer MacDonald Subject: Re: [Histonet] BS in Histotechnology It is the only truth I deal with here.? We are, like TJH, University medical school and they only care about the degree, four year is best.? They (administration and/or the pathologists) have never attempted to learn what we have or how we do it?and I doubt they will ever want to learn about Histology.? ? When I started many years ago the residents had to come through Histology for two to six weeks depending on the site.? Now we get 10 minutes to explain what they need to do to get good, not even great slides and stains. They simply are not interested and these will be the people future Histologists have to work for and depend on for pay.? We are in trouble and it is getting deeper.? I have the same question I have had for years: Where is NSH and how are they helping us move forward?? I have seen no movement to help get us raised to Laboratory Professionals.??I have only heard as long as we don't have degrees for our training we will not be recoginzied.? I have the degrees and still have to fight for salary and my rasies while if I were an MT it would be a given.? ? Sorry this is a sore subject and I fight yearly to get bare minimum raises for our people.? We did not get raises at all for two years and that was throughout the labs and hosptial.? Two percent raises are very close to an insult for us.? (We are talking angstrom close; not inches.) ? Pam ----- Original Message ----- From: "Sue" To: "Timothy Morken" Cc: "Histonet" , "Jennifer MacDonald" Sent: Tuesday, March 24, 2015 11:59:20 AM Subject: Re: [Histonet] BS in Histotechnology This is a fight that we continue to have with hospital administration.? In my opinion histologists are just as important and needed as MT.? Even though there is an increase in automation in pathology the hands on of a histologists is most important.? The fact that hospital still consider a lower entry job is the reason there are not more of us.? It is quite frustrating. ? Sue TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mundayscott <@t> gmavt.net Tue Mar 24 13:43:01 2015 From: mundayscott <@t> gmavt.net (scott munday) Date: Tue Mar 24 13:43:03 2015 Subject: [Histonet] Is your lab in need of a new microscope? Message-ID: We have several Olympus BX40 and BX41 compound microscopes in stock! and offer a 1 year warranty on all scopes. All microscopes are fully refurbished and are priced at 40% off list. The scopes are serviced by an Authorized Olympus Service Tech before sold. Please Email or call with questions. -- Scott Munday Munday Scientific Instrument Service 90 Misha Lane Sanford, NC 27330 Phone: 919-775-5596 Fax: 919-776-9566 From mucram11 <@t> comcast.net Tue Mar 24 13:45:08 2015 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Tue Mar 24 13:45:15 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <761E2B5697F795489C8710BCC72141FF367FF220@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <384355666.3717642.1427217151604.JavaMail.zimbra@comcast.net> <761E2B5697F795489C8710BCC72141FF367FF220@ex07.net.ucsf.edu> Message-ID: <714373262.3743896.1427222708004.JavaMail.zimbra@comcast.net> I agree and I am questioning NSH and what it is doing for us.? I support them mainly because they are creating some education routes for people who need CEUs.? I prefer to spent my time with state and regional societies in Histology as they are attempting to find ways to attract more people to Histology.? We all have that fight and finding ways to be recognized it not easy.? I lived in San Franciso during the early 80s and it is a difficult market with hisgh goals.? I am glad it is improving the status the Histologist there by being a corwded market where you can ask for better trained people and hold them to a path to improve even more. ? Pam ----- Original Message ----- From: "Timothy Morken" To: "Pam Marcum" , "Sue" Cc: "Histonet" , "Jennifer MacDonald" Sent: Tuesday, March 24, 2015 1:35:30 PM Subject: RE: [Histonet] BS in Histotechnology Pam, true enough. Indeed, for the annual NSH survey my only comment was that NSH has been ineffective in convincing pathology departments of the value of an HT or HTL certification - to the point that many are now questioning its value at all. Ours is ?one of few institutions that requires certification for advancement and our medical directors have been pushing for higher quality staff in order to raise the quality of our lab. We went through a pay revision about 7 years ago because the biotech companies and other large medical institutions were sucking up any candidates that poked their head up. We are now on par but still have to fight for any good candidates. But as long as histotechs are on the job trained (probably 99.9% now, as in the past), and invisible to high school and college students, the pay is going nowhere. It is still quite possible to get into the field with no experience . One of our techs got into histology by answering a Craig's list ad placed by a slide mill. He is ?very good tech, and has a degree in cellular and molecular biology, but that just goes to show how random our source pool is. His education is good, his histology training is random. And another quality candidate just randomly poked his head in my office a few months ago saying he had been doing some histology work in a research lab and was really excited to find out it could be a full time permanent job. We hired him but he is starting in accessioning ?and will work his way into histology. ?This is how most people get into histology. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center Tim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pam Marcum Sent: Tuesday, March 24, 2015 10:13 AM To: Sue Cc: Histonet; Morken, Timothy; Jennifer MacDonald Subject: Re: [Histonet] BS in Histotechnology It is the only truth I deal with here.? We are, like TJH, University medical school and they only care about the degree, four year is best.? They (administration and/or the pathologists) have never attempted to learn what we have or how we do it?and I doubt they will ever want to learn about Histology.? ? When I started many years ago the residents had to come through Histology for two to six weeks depending on the site.? Now we get 10 minutes to explain what they need to do to get good, not even great slides and stains. They simply are not interested and these will be the people future Histologists have to work for and depend on for pay.? We are in trouble and it is getting deeper.? I have the same question I have had for years: Where is NSH and how are they helping us move forward?? I have seen no movement to help get us raised to Laboratory Professionals.??I have only heard as long as we don't have degrees for our training we will not be recoginzied.? I have the degrees and still have to fight for salary and my rasies while if I were an MT it would be a given.? ? Sorry this is a sore subject and I fight yearly to get bare minimum raises for our people.? We did not get raises at all for two years and that was throughout the labs and hosptial.? Two percent raises are very close to an insult for us.? (We are talking angstrom close; not inches.) ? Pam ----- Original Message ----- From: "Sue" To: "Timothy Morken" Cc: "Histonet" , "Jennifer MacDonald" Sent: Tuesday, March 24, 2015 11:59:20 AM Subject: Re: [Histonet] BS in Histotechnology This is a fight that we continue to have with hospital administration.? In my opinion histologists are just as important and needed as MT.? Even though there is an increase in automation in pathology the hands on of a histologists is most important.? The fact that hospital still consider a lower entry job is the reason there are not more of us.? It is quite frustrating. ? Sue TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JRobinson <@t> pathology-associates.com Tue Mar 24 14:05:16 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Tue Mar 24 14:05:28 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8EAD5@PAEXCH1.PathologyAssociates.local> My administration did do a salary survey a few years back but it turned out only to ensure that we were within the "range" of the survey and not totally below the curve. I was told "no adjustments" would be made based on this salary survey. Salary ranges are a tightly held secret. I am the only HTL here so I only know my own salary range. They wouldn't tell me the salary range of the basic "histotech" position when I asked. I have argued for years that we cannot entice staff to take the initiative to get their certifications when they do not even know what the next step salary range is. A previous hospital where I worked (a county facility) only gave one additional pay step (5%) for having an HT or HTL. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 24, 2015 10:53 AM To: 'Sanders, Jeanine (CDC/OID/NCEZID)'; Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology That was nicer than the pathologist who told me years ago, "any monkey could be trained to do my job". I basically did not take the job I was interviewing for at the time. At least the next interview went a lot better and I did take the job. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 24, 2015 12:30 PM To: Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can teach any trained dog how to section histology will never have the recognition those of us that have studied and trained deserve. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 24, 2015 12:59 PM To: Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: Re: [Histonet] BS in Histotechnology This is a fight that we continue to have with hospital administration. In my opinion histologists are just as important and needed as MT. Even though there is an increase in automation in pathology the hands on of a histologists is most important. The fact that hospital still consider a lower entry job is the reason there are not more of us. It is quite frustrating. Sue TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From jqb7 <@t> cdc.gov Tue Mar 24 14:14:11 2015 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue Mar 24 14:14:38 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu>, Message-ID: <3B2CD438E1628A41BD687E98B963B78137F1811D@EMBX-CLFT4.cdc.gov> I know someone personally that works in a hospital and it hast Histotechnologist by his name....and he never took the HTL exam. He said his hospital bases it on experience ________________________________________ From: Carl Nituda [Cnituda@nvdermatology.com] Sent: Tuesday, March 24, 2015 2:32 PM To: Marcum, Pamela A; Sanders, Jeanine (CDC/OID/NCEZID); Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I personally think that a person just can't call themselves a Histotechnologist unless they went to school, training, and then pass the BOC by ASCP. Anyone, I mean anyone can perform a job with proper training in any field but that doesn't mean they should have that title until they pass certification. For hiring managers, I encourage you to hire certified candidates as priority and call them a Histotechnician, or Histotechnologist based on their certification. If a person is doing Histology work and is uncertified, encourage them to be certified and just don't give them a title. Imagine a world when people doing the job is actually certified like other professions, then you will get the respect from your colleagues that you deserve. Changes for the future of the profession starts with good leaders. Have a good and blessed week everyone. Carl Nituda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 24, 2015 10:53 AM To: 'Sanders, Jeanine (CDC/OID/NCEZID)'; Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology That was nicer than the pathologist who told me years ago, "any monkey could be trained to do my job". I basically did not take the job I was interviewing for at the time. At least the next interview went a lot better and I did take the job. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 24, 2015 12:30 PM To: Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can teach any trained dog how to section histology will never have the recognition those of us that have studied and trained deserve. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 24, 2015 12:59 PM To: Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: Re: [Histonet] BS in Histotechnology This is a fight that we continue to have with hospital administration. In my opinion histologists are just as important and needed as MT. Even though there is an increase in automation in pathology the hands on of a histologists is most important. The fact that hospital still consider a lower entry job is the reason there are not more of us. It is quite frustrating. Sue TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From b-frederick <@t> northwestern.edu Tue Mar 24 14:27:27 2015 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Tue Mar 24 14:27:35 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <3B2CD438E1628A41BD687E98B963B78137F1811D@EMBX-CLFT4.cdc.gov> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu>, <3B2CD438E1628A41BD687E98B963B78137F1811D@EMBX-CLFT4.cdc.gov> Message-ID: <951d69b8430d410982e45b4e57d21274@evcspmbx03.ads.northwestern.edu> "They" don't realize the theory we have to learn and those questions we have to answer like " What's the best fixative for a pheochromocytoma?" You tell them and they say the pathologist says B-5, to which I said, well they wouldn't pass out registry exam with that answer.....Grrr. Or the difference between a Mucin, Pas and Alcian Blue. The cytopath who asked did really need to know. As well I vaguely recall a question back on my HTL exam asking why a pathologist would request a mucin stain.... Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 24, 2015 2:14 PM To: Carl Nituda; Marcum, Pamela A; Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I know someone personally that works in a hospital and it hast Histotechnologist by his name....and he never took the HTL exam. He said his hospital bases it on experience ________________________________________ From: Carl Nituda [Cnituda@nvdermatology.com] Sent: Tuesday, March 24, 2015 2:32 PM To: Marcum, Pamela A; Sanders, Jeanine (CDC/OID/NCEZID); Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I personally think that a person just can't call themselves a Histotechnologist unless they went to school, training, and then pass the BOC by ASCP. Anyone, I mean anyone can perform a job with proper training in any field but that doesn't mean they should have that title until they pass certification. For hiring managers, I encourage you to hire certified candidates as priority and call them a Histotechnician, or Histotechnologist based on their certification. If a person is doing Histology work and is uncertified, encourage them to be certified and just don't give them a title. Imagine a world when people doing the job is actually certified like other professions, then you will get the respect from your colleagues that you deserve. Changes for the future of the profession starts with good leaders. Have a good and blessed week everyone. Carl Nituda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 24, 2015 10:53 AM To: 'Sanders, Jeanine (CDC/OID/NCEZID)'; Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology That was nicer than the pathologist who told me years ago, "any monkey could be trained to do my job". I basically did not take the job I was interviewing for at the time. At least the next interview went a lot better and I did take the job. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 24, 2015 12:30 PM To: Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can teach any trained dog how to section histology will never have the recognition those of us that have studied and trained deserve. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 24, 2015 12:59 PM To: Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: Re: [Histonet] BS in Histotechnology This is a fight that we continue to have with hospital administration. In my opinion histologists are just as important and needed as MT. Even though there is an increase in automation in pathology the hands on of a histologists is most important. The fact that hospital still consider a lower entry job is the reason there are not more of us. It is quite frustrating. Sue TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Tue Mar 24 14:29:08 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Tue Mar 24 14:29:14 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> Message-ID: OMG Pam~ our pathologist said the exact same thing to us when we started our Grossing training. Sheesh. . . Michael Ann On 3/24/15, 11:52 AM, "Marcum, Pamela A" wrote: >That was nicer than the pathologist who told me years ago, "any monkey >could be trained to do my job". I basically did not take the job I was >interviewing for at the time. At least the next interview went a lot >better and I did take the job. > >Pam > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, >Jeanine (CDC/OID/NCEZID) >Sent: Tuesday, March 24, 2015 12:30 PM >To: Sue; Timothy Morken >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald >Subject: RE: [Histonet] BS in Histotechnology > >I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can teach >any trained dog how to section histology will never have the recognition >those of us that have studied and trained deserve. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue >Sent: Tuesday, March 24, 2015 12:59 PM >To: Timothy Morken >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald >Subject: Re: [Histonet] BS in Histotechnology > >This is a fight that we continue to have with hospital administration. >In my opinion histologists are just as important and needed as MT. Even >though there is an increase in automation in pathology the hands on of a >histologists is most important. The fact that hospital still consider a >lower entry job is the reason there are not more of us. It is quite >frustrating. > >Sue >TJUH >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >---------------------------------------------------------------------- >Confidentiality Notice: This e-mail message, including any attachments, >is for the sole use of the intended recipient(s) and may contain >confidential and privileged information. Any unauthorized review, use, >disclosure or distribution is prohibited. If you are not the intended >recipient, please contact the sender by reply e-mail and destroy all >copies of the original message. From Cnituda <@t> nvdermatology.com Tue Mar 24 13:32:31 2015 From: Cnituda <@t> nvdermatology.com (Carl Nituda) Date: Tue Mar 24 14:44:26 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> Message-ID: I personally think that a person just can't call themselves a Histotechnologist unless they went to school, training, and then pass the BOC by ASCP. Anyone, I mean anyone can perform a job with proper training in any field but that doesn't mean they should have that title until they pass certification. For hiring managers, I encourage you to hire certified candidates as priority and call them a Histotechnician, or Histotechnologist based on their certification. If a person is doing Histology work and is uncertified, encourage them to be certified and just don't give them a title. Imagine a world when people doing the job is actually certified like other professions, then you will get the respect from your colleagues that you deserve. Changes for the future of the profession starts with good leaders. Have a good and blessed week everyone. Carl Nituda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 24, 2015 10:53 AM To: 'Sanders, Jeanine (CDC/OID/NCEZID)'; Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology That was nicer than the pathologist who told me years ago, "any monkey could be trained to do my job". I basically did not take the job I was interviewing for at the time. At least the next interview went a lot better and I did take the job. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 24, 2015 12:30 PM To: Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can teach any trained dog how to section histology will never have the recognition those of us that have studied and trained deserve. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 24, 2015 12:59 PM To: Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: Re: [Histonet] BS in Histotechnology This is a fight that we continue to have with hospital administration.? In my opinion histologists are just as important and needed as MT.? Even though there is an increase in automation in pathology the hands on of a histologists is most important.? The fact that hospital still consider a lower entry job is the reason there are not more of us.? It is quite frustrating. ? Sue TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mbourgeois <@t> finantempleton.com Tue Mar 24 14:42:46 2015 From: mbourgeois <@t> finantempleton.com (Michael Bourgeois) Date: Tue Mar 24 14:46:00 2015 Subject: [Histonet] Employment Opportunity Atlanta Area Message-ID: <000201d0666a$b436ef50$1ca4cdf0$@finantempleton.com> Finan Templeton Dermatopathology Associates is seeking a full-time certified Histotechnician to join our incredible team. Along with competitive wages, we offer Medical Insurance, Dental Insurance, Vision Insurance, Profit Sharing, and other benefits. To be considered for the position email your resume to MDB@FinanTempleton.com . Thank You, Michael Bourgeois Lab Manager/ Human Resources Manager mdb@finantempleton.com 1200 Lake Hearn Drive, Suite 300 | Atlanta, GA 30319 IMPORTANT/CONFIDENTIAL: This message and any attachments to it contain information from Finan Templeton., that is intended only for the use of the individual or entity named as the recipient. Such information is intended to be privileged, confidential, and exempt from disclosure under the applicable law. If the reader of this message is not the intended recipient or the employee or other agent responsible for delivering the message and any attachments to the intended recipient, you are hereby notified that any use, dissemination,distribution, or copying of this message and any attachments to it is strictly prohibited. If you have received this message and any attachments in error, please notify us immediately by telephone at: 404-851-1766 and permanently delete the message and any attachments from your e-mail system. Finan Templeton does not accept responsibility for changes to communications that occur after they have been sent. Thank you. From patpxs <@t> gmail.com Tue Mar 24 14:47:12 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Tue Mar 24 14:47:15 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> Message-ID: I've had more than one pathologist tell me a monkey could do my job. Though one of them said it with a smile and added "a very highly skilled and well trained monkey", he was one of the few who knew better. How many of us monkeys have trained the whining and complaining residents how to do things correctly? Paula On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones wrote: > OMG Pam~ our pathologist said the exact same thing to us when we started > our Grossing training. > Sheesh. . . > Michael Ann > > > > > On 3/24/15, 11:52 AM, "Marcum, Pamela A" wrote: > > >That was nicer than the pathologist who told me years ago, "any monkey > >could be trained to do my job". I basically did not take the job I was > >interviewing for at the time. At least the next interview went a lot > >better and I did take the job. > > > >Pam > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, > >Jeanine (CDC/OID/NCEZID) > >Sent: Tuesday, March 24, 2015 12:30 PM > >To: Sue; Timothy Morken > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >Subject: RE: [Histonet] BS in Histotechnology > > > >I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can teach > >any trained dog how to section histology will never have the recognition > >those of us that have studied and trained deserve. > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue > >Sent: Tuesday, March 24, 2015 12:59 PM > >To: Timothy Morken > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >Subject: Re: [Histonet] BS in Histotechnology > > > >This is a fight that we continue to have with hospital administration. > >In my opinion histologists are just as important and needed as MT. Even > >though there is an increase in automation in pathology the hands on of a > >histologists is most important. The fact that hospital still consider a > >lower entry job is the reason there are not more of us. It is quite > >frustrating. > > > >Sue > >TJUH > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >---------------------------------------------------------------------- > >Confidentiality Notice: This e-mail message, including any attachments, > >is for the sole use of the intended recipient(s) and may contain > >confidential and privileged information. Any unauthorized review, use, > >disclosure or distribution is prohibited. If you are not the intended > >recipient, please contact the sender by reply e-mail and destroy all > >copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PAMarcum <@t> uams.edu Tue Mar 24 15:05:37 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Mar 24 15:05:45 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <3B2CD438E1628A41BD687E98B963B78137F1811D@EMBX-CLFT4.cdc.gov> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu>, <3B2CD438E1628A41BD687E98B963B78137F1811D@EMBX-CLFT4.cdc.gov> Message-ID: <5de05aa905894f72a932c7b4aa55adca@MAIL13M2N2.ad.uams.edu> The really funny thing to me is, I know people who were OJT and no high school, just experience and hard work that I would trust with my tissue further than some of the newly trained degreed techs I have met. These are people who decided Histology was their field and career and worked to learn more than anyone ever expected when they took them in and trained them from a much lower level in the lab or hospital. They went on to learn IHC and anything else you could throw at them and at the lowest possible salary range. Those are the people who make me proud and after 50 years in various areas of the field I still would hire and work with in a heartbeat. Now times have changed and we need to advance the education and the fire that makes them want to excel in Histology as it continues to grow and advance. Despite the people who said molecular would replace or a machine in the 80s we are still needed and growing. We just need to get the word out to the next generations that we are a great field however; we must first find a way to get the salary level up. Unfortunately, that mean we are short people even longer as the two and four year degree requirements kick in. The fact that no one in a Biology or Science degree program had ever heard of Histology as a career in clinical and research is our biggest obstacle to start. We have sat quietly too long waiting for NSH and ASCP to help us get the recognition we deserve so we have to do ourselves by finding ways to drive home the need for HT/and HTL training in our fields. Pam Marcum -----Original Message----- From: Sanders, Jeanine (CDC/OID/NCEZID) [mailto:jqb7@cdc.gov] Sent: Tuesday, March 24, 2015 2:14 PM To: Carl Nituda; Marcum, Pamela A; Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I know someone personally that works in a hospital and it hast Histotechnologist by his name....and he never took the HTL exam. He said his hospital bases it on experience ________________________________________ From: Carl Nituda [Cnituda@nvdermatology.com] Sent: Tuesday, March 24, 2015 2:32 PM To: Marcum, Pamela A; Sanders, Jeanine (CDC/OID/NCEZID); Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I personally think that a person just can't call themselves a Histotechnologist unless they went to school, training, and then pass the BOC by ASCP. Anyone, I mean anyone can perform a job with proper training in any field but that doesn't mean they should have that title until they pass certification. For hiring managers, I encourage you to hire certified candidates as priority and call them a Histotechnician, or Histotechnologist based on their certification. If a person is doing Histology work and is uncertified, encourage them to be certified and just don't give them a title. Imagine a world when people doing the job is actually certified like other professions, then you will get the respect from your colleagues that you deserve. Changes for the future of the profession starts with good leaders. Have a good and blessed week everyone. Carl Nituda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 24, 2015 10:53 AM To: 'Sanders, Jeanine (CDC/OID/NCEZID)'; Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology That was nicer than the pathologist who told me years ago, "any monkey could be trained to do my job". I basically did not take the job I was interviewing for at the time. At least the next interview went a lot better and I did take the job. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 24, 2015 12:30 PM To: Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can teach any trained dog how to section histology will never have the recognition those of us that have studied and trained deserve. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 24, 2015 12:59 PM To: Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: Re: [Histonet] BS in Histotechnology This is a fight that we continue to have with hospital administration. In my opinion histologists are just as important and needed as MT. Even though there is an increase in automation in pathology the hands on of a histologists is most important. The fact that hospital still consider a lower entry job is the reason there are not more of us. It is quite frustrating. Sue TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From joelleweaver <@t> hotmail.com Tue Mar 24 15:06:35 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Tue Mar 24 15:06:40 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> References: , <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu>, <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> Message-ID: For what its worth, in my entire career, the pay has never been the same for an HTL with a bachelors and the same experience as any MT, and sometimes less than an MLT. I think maybe only once or twice I did get paid more ( like 5 cents) for having an HTL versus HT. Hospitals are horrible about that in general. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tpodawiltz@lrgh.org > To: Timothy.Morken@ucsf.edu; JMacDonald@mtsac.edu; histonet@lists.utsouthwestern.edu > Date: Tue, 24 Mar 2015 12:06:13 -0400 > Subject: RE: [Histonet] BS in Histotechnology > CC: > > So just out of curiosity is the pay on the same level as that of a Med Tech with a BS? > Does the BA/BS have to be in Histotechnology or is the BA/BS followed by one of the on-line certificate programs? > > Tom > > > Tom Podawiltz HT (ASCP) > AP Section Head > LRGHealthcare > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Tuesday, March 24, 2015 11:47 AM > To: Jennifer MacDonald; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] BS in Histotechnology > > Jennifer, we require a BA/BS degree for all Histotechnologist positions. However, in our 4 step categories Level 1 does not require certification, just the degree and the requirement that they get the certification within a year. Advancement to level 2 to 4 requires an HT or HTL certification (Level 1 = entry level bench tech, Level 2 is bench tech, level 3 is senior tech, level 4 is Lead tech). Supervisor requires and HTL. > > Considering that we already require a BA/BS degree for all levels, the fact a person has a HT or HTL is not going to matter much for levels 1 thru 4, only for supervisor level. > > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald > Sent: Monday, March 23, 2015 7:52 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] BS in Histotechnology > > In what areas would a facility hire an HTL over an HT? Is there a need for more HTL programs? 4 Thank you, _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > THIS MESSAGE IS CONFIDENTIAL. > > This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pmcdavid <@t> mhg.com Tue Mar 24 15:07:31 2015 From: Pmcdavid <@t> mhg.com (Patti McDavid) Date: Tue Mar 24 15:07:50 2015 Subject: [Histonet] BS in Histotechnology Message-ID: <1F886A89F4A53345928A11203C028957EC4C89E3@JANDC600-MBN01.mhg.local> Re: [Histonet] BS in Histotechnology Our laboratory decided when CLIA 88 came out, that we would require an associate's degree in science and a MLT or HT certification as a minimum to work in histology. The pay is the same for histology as in clinical departments. I strongly feel that was a good choice for our laboratory and hospital. I started with our facility as a MLT working in histology. Histology was a wonderful career path and did not impede my climb up the management ladder. Patti L. McDavid, Med, MLT/HTL (ASCP) Clinical Laboratory Manager 4500 Thirteenth Street P.O. Box 1810 Gulfport, MS 39502-1810 Phone 228-575-2340 Fax 228-865-3325 Pmcdavid@mhg.com [http://www.gulfportmemorial.com/images/14MH81-BESTE_SIG-RANKED-A-BESTD2.GIF] http://health.usnews.com/best-hospitals/area/ms ________________________________ This email may contain information covered under the Mississippi Privacy Law (Miss. Code Ann. ? 75-24-29), the Privacy Act of 1974 (5 U.S.C. ? 552a) and/or the Health Insurance Portability and Accountability Act of 1996 (Pub. L. No. 104-191) and its accompanying regulations. Healthcare information is personal and sensitive and must be protected in accordance with these provisions. If this email contains healthcare information, it is being disclosed to you only after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Re-disclosure without additional patient authorization, unless otherwise permitted by law, is prohibited. **********PRIVATE AND CONFIDENTIAL********** If you are not the intended recipient of this email, be advised that any use, disclosure, copying, distribution or taking any action in reliance on the contents of the information contained therein is strictly prohibited. If you have received this email in error, please contact the sender immediately by reply email and then destroy/delete all copies of the original message and any attachment(s) thereto. From bcooper <@t> chla.usc.edu Tue Mar 24 15:13:50 2015 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Tue Mar 24 15:13:57 2015 Subject: [Histonet] Pneumatic Tube Delivery System Message-ID: Dear Histonetters, For those facilities that have a pneumatic tube system in use, do any formalin fixed samples get delivered in this manner? The vast majority of our samples will not be, for obvious reasons. But there has been some discussion of combining bone marrow cores and aspirates and tubing them at the same time. The specimens will need to be physically separated of course because of the potential for damage of the aspirate/smear material due to exposure to formalin fumes. Have any of you crossed this bridge? I'd love to hear your experiences/concerns . . . Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper@chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From mturner <@t> CSILaboratories.com Tue Mar 24 15:26:04 2015 From: mturner <@t> CSILaboratories.com (Mark Turner) Date: Tue Mar 24 15:26:25 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> Message-ID: <643626B74DE2814D8537057F40E1A10B15506756@CSI-MX-NODEA.CSI-LABS.local> I once worked with a Pathologist who said she was in a group meeting of other pathologists when one of them blurted out that a trained monkey could cut slides. My pathologist, having had the opportunity to review some cases from the offender's laboratory, promptly replied "Yes, and with the quality of your slides it looks like you did just that." She shut down the other pathologist really quickly, and as far as I know, we never received another case to review from him. My pathologist was not about to let that kind of arrogance stand. She was one of the best bosses I ever had! Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, March 24, 2015 3:47 PM To: Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, Pamela A; Timothy Morken Subject: Re: [Histonet] BS in Histotechnology I've had more than one pathologist tell me a monkey could do my job. Though one of them said it with a smile and added "a very highly skilled and well trained monkey", he was one of the few who knew better. How many of us monkeys have trained the whining and complaining residents how to do things correctly? Paula On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones wrote: > OMG Pam~ our pathologist said the exact same thing to us when we > started our Grossing training. > Sheesh. . . > Michael Ann > > > > > On 3/24/15, 11:52 AM, "Marcum, Pamela A" wrote: > > >That was nicer than the pathologist who told me years ago, "any > >monkey could be trained to do my job". I basically did not take the > >job I was interviewing for at the time. At least the next interview > >went a lot better and I did take the job. > > > >Pam > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > >Sanders, Jeanine (CDC/OID/NCEZID) > >Sent: Tuesday, March 24, 2015 12:30 PM > >To: Sue; Timothy Morken > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >Subject: RE: [Histonet] BS in Histotechnology > > > >I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can > >teach any trained dog how to section histology will never have the > >recognition those of us that have studied and trained deserve. > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue > >Sent: Tuesday, March 24, 2015 12:59 PM > >To: Timothy Morken > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >Subject: Re: [Histonet] BS in Histotechnology > > > >This is a fight that we continue to have with hospital administration. > >In my opinion histologists are just as important and needed as MT. > >Even though there is an increase in automation in pathology the hands > >on of a histologists is most important. The fact that hospital still > >consider a lower entry job is the reason there are not more of us. > >It is quite frustrating. > > > >Sue > >TJUH > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >--------------------------------------------------------------------- > >- Confidentiality Notice: This e-mail message, including any > >attachments, is for the sole use of the intended recipient(s) and may > >contain confidential and privileged information. Any unauthorized > >review, use, disclosure or distribution is prohibited. If you are not > >the intended recipient, please contact the sender by reply e-mail and > >destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Tue Mar 24 15:27:23 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Mar 24 15:29:29 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <643626B74DE2814D8537057F40E1A10B15506756@CSI-MX-NODEA.CSI-LABS.local> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> <643626B74DE2814D8537057F40E1A10B15506756@CSI-MX-NODEA.CSI-LABS.local> Message-ID: Can we clone her????? -----Original Message----- From: Mark Turner [mailto:mturner@CSILaboratories.com] Sent: Tuesday, March 24, 2015 3:26 PM To: Paula Sicurello; Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, Pamela A; Timothy Morken Subject: RE: [Histonet] BS in Histotechnology I once worked with a Pathologist who said she was in a group meeting of other pathologists when one of them blurted out that a trained monkey could cut slides. My pathologist, having had the opportunity to review some cases from the offender's laboratory, promptly replied "Yes, and with the quality of your slides it looks like you did just that." She shut down the other pathologist really quickly, and as far as I know, we never received another case to review from him. My pathologist was not about to let that kind of arrogance stand. She was one of the best bosses I ever had! Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, March 24, 2015 3:47 PM To: Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, Pamela A; Timothy Morken Subject: Re: [Histonet] BS in Histotechnology I've had more than one pathologist tell me a monkey could do my job. Though one of them said it with a smile and added "a very highly skilled and well trained monkey", he was one of the few who knew better. How many of us monkeys have trained the whining and complaining residents how to do things correctly? Paula On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones wrote: > OMG Pam~ our pathologist said the exact same thing to us when we > started our Grossing training. > Sheesh. . . > Michael Ann > > > > > On 3/24/15, 11:52 AM, "Marcum, Pamela A" wrote: > > >That was nicer than the pathologist who told me years ago, "any > >monkey could be trained to do my job". I basically did not take the > >job I was interviewing for at the time. At least the next interview > >went a lot better and I did take the job. > > > >Pam > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > >Sanders, Jeanine (CDC/OID/NCEZID) > >Sent: Tuesday, March 24, 2015 12:30 PM > >To: Sue; Timothy Morken > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >Subject: RE: [Histonet] BS in Histotechnology > > > >I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can > >teach any trained dog how to section histology will never have the > >recognition those of us that have studied and trained deserve. > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue > >Sent: Tuesday, March 24, 2015 12:59 PM > >To: Timothy Morken > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >Subject: Re: [Histonet] BS in Histotechnology > > > >This is a fight that we continue to have with hospital administration. > >In my opinion histologists are just as important and needed as MT. > >Even though there is an increase in automation in pathology the hands > >on of a histologists is most important. The fact that hospital still > >consider a lower entry job is the reason there are not more of us. > >It is quite frustrating. > > > >Sue > >TJUH > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >--------------------------------------------------------------------- > >- Confidentiality Notice: This e-mail message, including any > >attachments, is for the sole use of the intended recipient(s) and may > >contain confidential and privileged information. Any unauthorized > >review, use, disclosure or distribution is prohibited. If you are not > >the intended recipient, please contact the sender by reply e-mail and > >destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From blayjorge <@t> gmail.com Tue Mar 24 15:38:58 2015 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Tue Mar 24 15:39:02 2015 Subject: [Histonet] Measuring cell length and surface area Message-ID: Hello Histonetter: A student working with me and I have completed a little project characterizing plant mutations whose expression is manifested in the size/shape of the cells. Typically, how many cells per specimen are measured? I was planning to digitally measure a sample of cells in each specimen (e.g. all cells located along the central axis and then the cells in one cross section approximately perpendicular to the first axis. Any constructive feedback, blayjorge@gmail.com , welcomed. Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From Kimberly <@t> animalreferencepathology.com Tue Mar 24 16:18:37 2015 From: Kimberly <@t> animalreferencepathology.com (Kimberly Marshall) Date: Tue Mar 24 16:18:46 2015 Subject: [Histonet] Tol Blue Message-ID: <1427231919955.28190@animalreferencepathology.com> ?Hello my fellow Histo Techs. Have a question I just know someone out there can answer for me. In canine tissue, we are having problems with the Tol Blue for mast cell. Am experiencing metachromasia, or the mast cells turning purple not blue. I have attempted to decrease time, or add time, but its not helping. My pathologist says he has had this issue before. So question is. Could it be the mast cell in a dog does not stain the same? Is there another stain that may work? Any help will be much appreciated. Thanks in Advance. Kimberly Marshall H.T.(ASCP) Kimberly Marshall H.T.(ASCP) Histology/Lab Supervisor Toll Free 1-800-426-2099 Fax 801-584-5104 PO Box 17580 Salt Lake City, Utah 84107 www.animalreferencepathology.com Advancing the art and science of veterinary medicine [cid:image001.jpg@01CF8F87.A0BD4830] From Nancy.Stedman <@t> buschgardens.com Tue Mar 24 16:22:26 2015 From: Nancy.Stedman <@t> buschgardens.com (Stedman, Nancy) Date: Tue Mar 24 16:22:33 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <643626B74DE2814D8537057F40E1A10B15506756@CSI-MX-NODEA.CSI-LABS.local> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> <643626B74DE2814D8537057F40E1A10B15506756@CSI-MX-NODEA.CSI-LABS.local> Message-ID: <18D791D4EE07BC41BF05F8EF3CCCDE24678E4765@FTCSEAP4001.nam.int.local> As a pathologist I'd like to apologize for all the pathologists who have made comments like this.. forget trained monkeys and dogs, most (all?) pathologists cannot cut slides either, at least not slides they'd want to try to read. I know I can't. -Nancy Stedman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Tuesday, March 24, 2015 4:26 PM To: Paula Sicurello; Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Timothy Morken; Jennifer MacDonald; Marcum, Pamela A Subject: RE: [Histonet] BS in Histotechnology I once worked with a Pathologist who said she was in a group meeting of other pathologists when one of them blurted out that a trained monkey could cut slides. My pathologist, having had the opportunity to review some cases from the offender's laboratory, promptly replied "Yes, and with the quality of your slides it looks like you did just that." She shut down the other pathologist really quickly, and as far as I know, we never received another case to review from him. My pathologist was not about to let that kind of arrogance stand. She was one of the best bosses I ever had! Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, March 24, 2015 3:47 PM To: Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, Pamela A; Timothy Morken Subject: Re: [Histonet] BS in Histotechnology I've had more than one pathologist tell me a monkey could do my job. Though one of them said it with a smile and added "a very highly skilled and well trained monkey", he was one of the few who knew better. How many of us monkeys have trained the whining and complaining residents how to do things correctly? Paula On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones wrote: > OMG Pam~ our pathologist said the exact same thing to us when we > started our Grossing training. > Sheesh. . . > Michael Ann > > > > > On 3/24/15, 11:52 AM, "Marcum, Pamela A" wrote: > > >That was nicer than the pathologist who told me years ago, "any > >monkey could be trained to do my job". I basically did not take the > >job I was interviewing for at the time. At least the next interview > >went a lot better and I did take the job. > > > >Pam > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > >Sanders, Jeanine (CDC/OID/NCEZID) > >Sent: Tuesday, March 24, 2015 12:30 PM > >To: Sue; Timothy Morken > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >Subject: RE: [Histonet] BS in Histotechnology > > > >I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can > >teach any trained dog how to section histology will never have the > >recognition those of us that have studied and trained deserve. > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue > >Sent: Tuesday, March 24, 2015 12:59 PM > >To: Timothy Morken > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >Subject: Re: [Histonet] BS in Histotechnology > > > >This is a fight that we continue to have with hospital administration. > >In my opinion histologists are just as important and needed as MT. > >Even though there is an increase in automation in pathology the hands > >on of a histologists is most important. The fact that hospital still > >consider a lower entry job is the reason there are not more of us. > >It is quite frustrating. > > > >Sue > >TJUH > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >--------------------------------------------------------------------- > >- Confidentiality Notice: This e-mail message, including any > >attachments, is for the sole use of the intended recipient(s) and may > >contain confidential and privileged information. Any unauthorized > >review, use, disclosure or distribution is prohibited. If you are not > >the intended recipient, please contact the sender by reply e-mail and > >destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Tue Mar 24 16:30:29 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Tue Mar 24 16:30:39 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <18D791D4EE07BC41BF05F8EF3CCCDE24678E4765@FTCSEAP4001.nam.int.local> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> <643626B74DE2814D8537057F40E1A10B15506756@CSI-MX-NODEA.CSI-LABS.local> <18D791D4EE07BC41BF05F8EF3CCCDE24678E4765@FTCSEAP4001.nam.int.local> Message-ID: Thanks Dr. Stedman! Good to hear! Michael Ann On 3/24/15, 3:22 PM, "Stedman, Nancy" wrote: >As a pathologist I'd like to apologize for all the pathologists who have >made comments like this.. forget trained monkeys and dogs, most (all?) >pathologists cannot cut slides either, at least not slides they'd want to >try to read. I know I can't. > >-Nancy Stedman > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark >Turner >Sent: Tuesday, March 24, 2015 4:26 PM >To: Paula Sicurello; Michael Ann Jones >Cc: histonet@lists.utsouthwestern.edu; Timothy Morken; Jennifer >MacDonald; Marcum, Pamela A >Subject: RE: [Histonet] BS in Histotechnology > >I once worked with a Pathologist who said she was in a group meeting of >other pathologists when one of them blurted out that a trained monkey >could cut slides. My pathologist, having had the opportunity to review >some cases from the offender's laboratory, promptly replied "Yes, and >with the quality of your slides it looks like you did just that." She >shut down the other pathologist really quickly, and as far as I know, we >never received another case to review from him. My pathologist was not >about to let that kind of arrogance stand. She was one of the best >bosses I ever had! > >Mark Turner, Ph.D., HT(ASCP)QIHC >Manager, Histology/IHC > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula >Sicurello >Sent: Tuesday, March 24, 2015 3:47 PM >To: Michael Ann Jones >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, Pamela >A; Timothy Morken >Subject: Re: [Histonet] BS in Histotechnology > >I've had more than one pathologist tell me a monkey could do my job. >Though one of them said it with a smile and added "a very highly skilled >and well trained monkey", he was one of the few who knew better. > >How many of us monkeys have trained the whining and complaining residents >how to do things correctly? > >Paula > >On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones >wrote: > >> OMG Pam~ our pathologist said the exact same thing to us when we >> started our Grossing training. >> Sheesh. . . >> Michael Ann >> >> >> >> >> On 3/24/15, 11:52 AM, "Marcum, Pamela A" wrote: >> >> >That was nicer than the pathologist who told me years ago, "any >> >monkey could be trained to do my job". I basically did not take the >> >job I was interviewing for at the time. At least the next interview >> >went a lot better and I did take the job. >> > >> >Pam >> > >> >-----Original Message----- >> >From: histonet-bounces@lists.utsouthwestern.edu >> >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> >Sanders, Jeanine (CDC/OID/NCEZID) >> >Sent: Tuesday, March 24, 2015 12:30 PM >> >To: Sue; Timothy Morken >> >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald >> >Subject: RE: [Histonet] BS in Histotechnology >> > >> >I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can >> >teach any trained dog how to section histology will never have the >> >recognition those of us that have studied and trained deserve. >> > >> >-----Original Message----- >> >From: histonet-bounces@lists.utsouthwestern.edu >> >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue >> >Sent: Tuesday, March 24, 2015 12:59 PM >> >To: Timothy Morken >> >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald >> >Subject: Re: [Histonet] BS in Histotechnology >> > >> >This is a fight that we continue to have with hospital administration. >> >In my opinion histologists are just as important and needed as MT. >> >Even though there is an increase in automation in pathology the hands >> >on of a histologists is most important. The fact that hospital still >> >consider a lower entry job is the reason there are not more of us. >> >It is quite frustrating. >> > >> >Sue >> >TJUH >> >_______________________________________________ >> >Histonet mailing list >> >Histonet@lists.utsouthwestern.edu >> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> >--------------------------------------------------------------------- >> >- Confidentiality Notice: This e-mail message, including any >> >attachments, is for the sole use of the intended recipient(s) and may >> >contain confidential and privileged information. Any unauthorized >> >review, use, disclosure or distribution is prohibited. If you are not >> >the intended recipient, please contact the sender by reply e-mail and >> >destroy all copies of the original message. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From suetp918 <@t> comcast.net Tue Mar 24 16:46:33 2015 From: suetp918 <@t> comcast.net (suetp918) Date: Tue Mar 24 16:46:54 2015 Subject: [Histonet] BS in Histotechnology Message-ID: <32d8vkgwkh0fmxaaa6fvcxr9.1427233593794@email.android.com> I agree Sent from my Verizon Wireless 4G LTE smartphone -------- Original message -------- From: Carl Nituda Date:03/24/2015 2:32 PM (GMT-05:00) To: "Marcum, Pamela A" , "'Sanders, Jeanine (CDC/OID/NCEZID)'" , Sue , Timothy Morken Cc: histonet@lists.utsouthwestern.edu, Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I personally think that a person just can't call themselves a Histotechnologist unless they went to school, training, and then pass the BOC by ASCP. Anyone, I mean anyone can perform a job with proper training in any field but that doesn't mean they should have that title until they pass certification. For hiring managers, I encourage you to hire certified candidates as priority and call them a Histotechnician, or Histotechnologist based on their certification. If a person is doing Histology work and is uncertified, encourage them to be certified and just don't give them a title. Imagine a world when people doing the job is actually certified like other professions, then you will get the respect from your colleagues that you deserve. Changes for the future of the profession starts with good leaders. Have a good and blessed week everyone. Carl Nituda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 24, 2015 10:53 AM To: 'Sanders, Jeanine (CDC/OID/NCEZID)'; Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology That was nicer than the pathologist who told me years ago, "any monkey could be trained to do my job". I basically did not take the job I was interviewing for at the time. At least the next interview went a lot better and I did take the job. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 24, 2015 12:30 PM To: Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can teach any trained dog how to section histology will never have the recognition those of us that have studied and trained deserve. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 24, 2015 12:59 PM To: Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: Re: [Histonet] BS in Histotechnology This is a fight that we continue to have with hospital administration. In my opinion histologists are just as important and needed as MT. Even though there is an increase in automation in pathology the hands on of a histologists is most important. The fact that hospital still consider a lower entry job is the reason there are not more of us. It is quite frustrating. Sue TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From suetp918 <@t> comcast.net Tue Mar 24 16:49:56 2015 From: suetp918 <@t> comcast.net (suetp918) Date: Tue Mar 24 16:50:07 2015 Subject: [Histonet] BS in Histotechnology Message-ID: So most admin foljs do not know abt us until someone in their family had a bx and needed a dx thsn all of a sudden we needed to jump thru hoops and they were our best rriend Sent from my Verizon Wireless 4G LTE smartphone -------- Original message -------- From: Bernice Frederick Date:03/24/2015 3:27 PM (GMT-05:00) To: "Sanders, Jeanine (CDC/OID/NCEZID)" , Carl Nituda , "Marcum, Pamela A" , Sue , Timothy Morken Cc: histonet@lists.utsouthwestern.edu, Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology "They" don't realize the theory we have to learn and those questions we have to answer like " What's the best fixative for a pheochromocytoma?" You tell them and they say the pathologist says B-5, to which I said, well they wouldn't pass out registry exam with that answer.....Grrr. Or the difference between a Mucin, Pas and Alcian Blue. The cytopath who asked did really need to know. As well I vaguely recall a question back on my HTL exam asking why a pathologist would request a mucin stain.... Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 24, 2015 2:14 PM To: Carl Nituda; Marcum, Pamela A; Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I know someone personally that works in a hospital and it hast Histotechnologist by his name....and he never took the HTL exam. He said his hospital bases it on experience ________________________________________ From: Carl Nituda [Cnituda@nvdermatology.com] Sent: Tuesday, March 24, 2015 2:32 PM To: Marcum, Pamela A; Sanders, Jeanine (CDC/OID/NCEZID); Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I personally think that a person just can't call themselves a Histotechnologist unless they went to school, training, and then pass the BOC by ASCP. Anyone, I mean anyone can perform a job with proper training in any field but that doesn't mean they should have that title until they pass certification. For hiring managers, I encourage you to hire certified candidates as priority and call them a Histotechnician, or Histotechnologist based on their certification. If a person is doing Histology work and is uncertified, encourage them to be certified and just don't give them a title. Imagine a world when people doing the job is actually certified like other professions, then you will get the respect from your colleagues that you deserve. Changes for the future of the profession starts with good leaders. Have a good and blessed week everyone. Carl Nituda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Tuesday, March 24, 2015 10:53 AM To: 'Sanders, Jeanine (CDC/OID/NCEZID)'; Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology That was nicer than the pathologist who told me years ago, "any monkey could be trained to do my job". I basically did not take the job I was interviewing for at the time. At least the next interview went a lot better and I did take the job. Pam -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 24, 2015 12:30 PM To: Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can teach any trained dog how to section histology will never have the recognition those of us that have studied and trained deserve. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue Sent: Tuesday, March 24, 2015 12:59 PM To: Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: Re: [Histonet] BS in Histotechnology This is a fight that we continue to have with hospital administration. In my opinion histologists are just as important and needed as MT. Even though there is an increase in automation in pathology the hands on of a histologists is most important. The fact that hospital still consider a lower entry job is the reason there are not more of us. It is quite frustrating. Sue TJUH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Mar 24 16:55:21 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Mar 24 16:55:45 2015 Subject: [Histonet] Tol Blue In-Reply-To: <1427231919955.28190@animalreferencepathology.com> References: <1427231919955.28190@animalreferencepathology.com> Message-ID: Mast cells granules are metachromatic. What you see is the expected staining reaction. From: Kimberly Marshall To: "histonet@lists.utsouthwestern.edu" Date: 03/24/2015 02:20 PM Subject: [Histonet] Tol Blue Sent by: histonet-bounces@lists.utsouthwestern.edu ?Hello my fellow Histo Techs. Have a question I just know someone out there can answer for me. In canine tissue, we are having problems with the Tol Blue for mast cell. Am experiencing metachromasia, or the mast cells turning purple not blue. I have attempted to decrease time, or add time, but its not helping. My pathologist says he has had this issue before. So question is. Could it be the mast cell in a dog does not stain the same? Is there another stain that may work? Any help will be much appreciated. Thanks in Advance. Kimberly Marshall H.T.(ASCP) Kimberly Marshall H.T.(ASCP) Histology/Lab Supervisor Toll Free 1-800-426-2099 Fax 801-584-5104 PO Box 17580 Salt Lake City, Utah 84107 www.animalreferencepathology.com Advancing the art and science of veterinary medicine [cid:image001.jpg@01CF8F87.A0BD4830] _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Tue Mar 24 17:00:55 2015 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Mar 24 17:01:03 2015 Subject: [Histonet] Tol Blue In-Reply-To: References: <1427231919955.28190@animalreferencepathology.com> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79F17F3D@SBS2K8.premierlab.local> On occasion you might lose the metachromatic staining with T. Blue if you if you dehydrate in alcohol. We normally air dry, place in xylene and coverslip. You will never run into a problem with the staining if you do that. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, March 24, 2015 3:55 PM To: Kimberly Marshall Cc: histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Tol Blue Mast cells granules are metachromatic. What you see is the expected staining reaction. From: Kimberly Marshall To: "histonet@lists.utsouthwestern.edu" Date: 03/24/2015 02:20 PM Subject: [Histonet] Tol Blue Sent by: histonet-bounces@lists.utsouthwestern.edu ?Hello my fellow Histo Techs. Have a question I just know someone out there can answer for me. In canine tissue, we are having problems with the Tol Blue for mast cell. Am experiencing metachromasia, or the mast cells turning purple not blue. I have attempted to decrease time, or add time, but its not helping. My pathologist says he has had this issue before. So question is. Could it be the mast cell in a dog does not stain the same? Is there another stain that may work? Any help will be much appreciated. Thanks in Advance. Kimberly Marshall H.T.(ASCP) Kimberly Marshall H.T.(ASCP) Histology/Lab Supervisor Toll Free 1-800-426-2099 Fax 801-584-5104 PO Box 17580 Salt Lake City, Utah 84107 www.animalreferencepathology.com Advancing the art and science of veterinary medicine [cid:image001.jpg@01CF8F87.A0BD4830] _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Tue Mar 24 17:12:47 2015 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Mar 24 17:13:22 2015 Subject: [Histonet] Tol Blue In-Reply-To: <1427231919955.28190@animalreferencepathology.com> References: <1427231919955.28190@animalreferencepathology.com> Message-ID: <5511E15F.30801@shaw.ca> Try the method at: http://stainsfile.info/StainsFile/stain/cell/aldtolblue.htm Bryan Llewellyn Kimberly Marshall wrote: > ?Hello my fellow Histo Techs. Have a question I just know someone out there can answer for me. > > In canine tissue, we are having problems with the Tol Blue for mast cell. Am experiencing metachromasia, or the mast cells turning purple not blue. I have attempted to decrease time, or add time, but its not helping. My pathologist says he has had this issue before. So question is. Could it be the mast cell in a dog does not stain the same? Is there another stain that may work? Any help will be much appreciated. > > > Thanks in Advance. > > Kimberly Marshall H.T.(ASCP) > > > > > > > Kimberly Marshall H.T.(ASCP) > > Histology/Lab Supervisor > > Toll Free 1-800-426-2099 > > Fax 801-584-5104 > > PO Box 17580 > > Salt Lake City, Utah 84107 > > www.animalreferencepathology.com > > > > Advancing the art and science of veterinary medicine > > > > [cid:image001.jpg@01CF8F87.A0BD4830] > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From wsimons <@t> athensgastro.com Tue Mar 24 17:15:06 2015 From: wsimons <@t> athensgastro.com (wsimons@athensgastro.com) Date: Tue Mar 24 17:15:11 2015 Subject: =?utf-8?B?UmU6IFtIaXN0b25ldF0gQlMgaW4gSGlzdG90ZWNobm9sb2d5?= In-Reply-To: <1F886A89F4A53345928A11203C028957EC4C89E3@JANDC600-MBN01.mhg.local> References: <1F886A89F4A53345928A11203C028957EC4C89E3@JANDC600-MBN01.mhg.local> Message-ID: <20150324221507.10851.qmail@server276.com> This is a great example of what can be accomplished as a HistoPROFESSIONAL. I commend you Patti and the bottom line is "take responsibility" for your own career path/ladder. Two things as HISTOPROFESSIONALS we can contribute, continuing education and to "pay it forward". My personal experience has been rewarding because of two contributors to my learning curve, my mentors and my "dementors". I encountered pathologisits who mentored me, but the ones that did not value me did not deter me. Until we value ourselves and address unhealthy situations we will never move forward. Sometimes that means leaving our comfort zone or the convenient location. And in my experience sometimes being "pushed out of the nest". I obtained my first AS through distance learning with an award from the NSH. My second associate culminated with travel abroad in Cusco, Peru. I have a ridiculous amount of semester hours, but no BS....yet. Bottom line is VALUE your own worth, continue your education, educate others and if you are in a "hamster" situation jump off that wheel! I'm sure I will get feedback ...."the grass is not always greener", but how about this idiom "why buy the cow when you can get the milk for free". It's not even Friday yet~ Wanda K. Simons, HT (ASCP) Artist & Scientist for Histotechnology > -------Original Message------- > From: Patti McDavid > To: 'histonet@lists.utsouthwestern.edu' > Subject: Re: [Histonet] BS in Histotechnology > Sent: Mar 24 '15 16:11 > > Re: [Histonet] BS in Histotechnology > Our laboratory decided when CLIA 88 came out, that we would require an associate's degree in science and a MLT or HT certification as a minimum > to work in histology.??The pay is the same for histology as in clinical departments.??I strongly feel that was a good choice for our laboratory and hospital. > I started with our facility as a MLT working in histology.??Histology was a wonderful career path and did not impede my climb up the management ladder. > > Patti L. McDavid, Med, MLT/HTL (ASCP) > Clinical Laboratory Manager > 4500 Thirteenth Street > P.O. Box 1810 > Gulfport, MS??39502-1810 > Phone 228-575-2340 > Fax 228-865-3325 > Pmcdavid@mhg.com > > > > > [http://www.gulfportmemorial.com/images/14MH81-BESTE_SIG-RANKED-A-BESTD2.GIF] > http://health.usnews.com/best-hospitals/area/ms > ________________________________ > This email may contain information covered under the Mississippi Privacy Law (Miss. Code Ann. ? 75-24-29), the Privacy Act of 1974 (5 U.S.C. ? 552a) and/or the Health Insurance Portability and Accountability Act of 1996 (Pub. L. No. 104-191) and its accompanying regulations. Healthcare information is personal and sensitive and must be protected in accordance with these provisions. If this email contains healthcare information, it is being disclosed to you only after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Re-disclosure without additional patient authorization, unless otherwise permitted by law, is prohibited. > > **********PRIVATE AND CONFIDENTIAL********** > > If you are not the intended recipient of this email, be advised that any use, disclosure, copying, distribution or taking any action in reliance on the contents of the information contained therein is strictly prohibited. If you have received this email in error, please contact the sender immediately by reply email and then destroy/delete all copies of the original message and any attachment(s) thereto. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From linda.prasad <@t> health.nsw.gov.au Tue Mar 24 17:52:38 2015 From: linda.prasad <@t> health.nsw.gov.au (Linda Prasad (SCHN)) Date: Tue Mar 24 17:53:12 2015 Subject: [Histonet] Tol Blue In-Reply-To: <5511E15F.30801@shaw.ca> References: <1427231919955.28190@animalreferencepathology.com> <5511E15F.30801@shaw.ca> Message-ID: <1217DDB3D7DE5E418E3D560A268EABD0E0E86239@xmdb03.nch.kids> The mast cells are meant to turn purple. It?s a metachromatic stain. So the tol blue stains the tissue blue and the mast cells purple. Mast cell granules Purple Acid Mucosubstance Purple Nuclei Blue Mast cells are rich in heparin. This allows them to be stained via acid mucopolysaccharide and metachromatic staining techniques. Churukian and Schenk developed this metachromatic dye-technique to reduce the excessive background staining that is common with metachromatic dye techniques. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???Please consider the environment before printing this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Llewellyn Sent: Wednesday, 25 March 2015 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Tol Blue Try the method at: http://stainsfile.info/StainsFile/stain/cell/aldtolblue.htm Bryan Llewellyn Kimberly Marshall wrote: > ?Hello my fellow Histo Techs. Have a question I just know someone out there can answer for me. > > In canine tissue, we are having problems with the Tol Blue for mast cell. Am experiencing metachromasia, or the mast cells turning purple not blue. I have attempted to decrease time, or add time, but its not helping. My pathologist says he has had this issue before. So question is. Could it be the mast cell in a dog does not stain the same? Is there another stain that may work? Any help will be much appreciated. > > > Thanks in Advance. > > Kimberly Marshall H.T.(ASCP) > > > > > > > Kimberly Marshall H.T.(ASCP) > > Histology/Lab Supervisor > > Toll Free 1-800-426-2099 > > Fax 801-584-5104 > > PO Box 17580 > > Salt Lake City, Utah 84107 > > www.animalreferencepathology.com > > > > Advancing the art and science of veterinary medicine > > > > [cid:image001.jpg@01CF8F87.A0BD4830] > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From barryrittman <@t> gmail.com Tue Mar 24 20:36:19 2015 From: barryrittman <@t> gmail.com (Barry Rittman) Date: Tue Mar 24 20:36:22 2015 Subject: [Histonet] measuring cells Message-ID: I apologize for misplacing the original posting about this. Measuring the number of cells in sections is very tricky and varies considerably with the thickness of the section and the size of the cells. In general the thicker the section the more accurate the count as there are more complete cells rather than just profiles. The problem is that in order to get a really accurate count you need sections that are so thick that resolution suffers. Abercrombie in 1946 published papers that took into account the size of cells and the thickness of the sections and depending on these applied a correction factor that improved the accuracy of the count. His papers are worth reading. The link is below for those who are interested. http://www.nervenet.org/papers/Abercrombie46.html Of course an interesting approach would be to cut several sections, separate the cells and then use a Coulter counter... like this is going to happen!! Barry From tpodawiltz <@t> lrgh.org Wed Mar 25 05:18:51 2015 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Wed Mar 25 05:18:57 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> Message-ID: <38667E7FB77ECD4E91BFAEB8D98638632616514332@LRGHEXVS1.practice.lrgh.org> Tim for a Pathology Manager you seem to have a low opinion of the education and training of the Histo Techs that work for you. Is your training program accredited with one of the Histology schools or is your staff left to rend for themselves? By the way, the lab that I work at basis the starting salaries on your degree first, then specialty so MT, HT/HTL with BS degrees earn the same. Tom -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsf.edu] Sent: Tuesday, March 24, 2015 12:42 PM To: Podawiltz, Thomas; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Tom, no, Histo does start lower than med techs, but consider that a med tech has specialty training from the time they decide to go that route while most histotechs have general biology degrees and nothing but on the job training. Even with a certification a Histotech is not at the same level as a med tech simply due to the unstructured nature of their self-education and training. In 30+ years I have met only a handful of people who got any sort of degrees in Histotechnology, so waiting for those people to come along is not going to work for hiring. Most of our staff got their certification while working here and did it on their own. Only one has a degree in Histotechnology, and a BS at that!. A starting salary here is $36/hr and it is a $3 to $4 increase per level. The lab staff is unionized, and we compete with many large service labs (ie Kaiser) and many, many large biotech companies for the same pool of techs. Plus, it is expensive to live in the San Francisco Bay Area. We only recently (a few years ago) started this requirement in order to get our staff to a higher level. We still have staff without BA/BS degrees. The degree just needs to meet the requirements for certification so does not need to be a specialty degree. Tim -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Tuesday, March 24, 2015 9:06 AM To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology So just out of curiosity is the pay on the same level as that of a Med Tech with a BS? Does the BA/BS have to be in Histotechnology or is the BA/BS followed by one of the on-line certificate programs? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, March 24, 2015 11:47 AM To: Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Jennifer, we require a BA/BS degree for all Histotechnologist positions. However, in our 4 step categories Level 1 does not require certification, just the degree and the requirement that they get the certification within a year. Advancement to level 2 to 4 requires an HT or HTL certification (Level 1 = entry level bench tech, Level 2 is bench tech, level 3 is senior tech, level 4 is Lead tech). Supervisor requires and HTL. Considering that we already require a BA/BS degree for all levels, the fact a person has a HT or HTL is not going to matter much for levels 1 thru 4, only for supervisor level. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, March 23, 2015 7:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BS in Histotechnology In what areas would a facility hire an HTL over an HT? Is there a need for more HTL programs? 4 Thank you, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From DKBoyd <@t> chs.net Wed Mar 25 06:36:48 2015 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Wed Mar 25 06:37:07 2015 Subject: [Histonet] RE: Pneumatic Tube Delivery System In-Reply-To: References: Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9F10D4D@TN001WEXMBX014.US.chs.net> Specimens that are irretrievable are not allowed in the pneumatic tube. Not only would it be painful and expensive if lost or broken in the tube, but the biohazard of a formalin leak, spill or breakage is too great. Smears can be broken as well exposing the staff to possible cuts with bloody smears. Spinal Fluid nor Body Fluids are allowed in the tube system, because of the procedure to procure it and the limited amount. Urine and blood are easy to obtain and plentiful. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Cooper, Brian [bcooper@chla.usc.edu] Sent: Tuesday, March 24, 2015 4:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pneumatic Tube Delivery System Dear Histonetters, For those facilities that have a pneumatic tube system in use, do any formalin fixed samples get delivered in this manner? The vast majority of our samples will not be, for obvious reasons. But there has been some discussion of combining bone marrow cores and aspirates and tubing them at the same time. The specimens will need to be physically separated of course because of the potential for damage of the aspirate/smear material due to exposure to formalin fumes. Have any of you crossed this bridge? I'd love to hear your experiences/concerns . . . Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper@chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Julia.Cates <@t> AHSS.ORG Wed Mar 25 07:58:40 2015 From: Julia.Cates <@t> AHSS.ORG (Cates, Julia) Date: Wed Mar 25 07:58:48 2015 Subject: [Histonet] Re: Pneumatic Tube Delivery System Message-ID: Hey Brian, We do use the tube system at our hospital. The rule here is that nothing in formalin is to be sent via tube. The specimen is considered irreplaceable and in the event of a malfunction in the system there is the possibility of specimen loss. Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. From PAMarcum <@t> uams.edu Wed Mar 25 08:24:10 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Mar 25 08:24:20 2015 Subject: [Histonet] RE: Re: Pneumatic Tube Delivery System In-Reply-To: References: Message-ID: <8a38b4c901f1454e8554da363b9c651b@MAIL13M2N2.ad.uams.edu> Exactly how we handle it here. Years ago they something biological spill in the tube system and the whole thing had to be decontaminated. No one ever volunteered who did it, but it cost a fortune to clean up. Still once in a whole someone will just have a brain hiccup and send something. Everyone get upset then. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cates, Julia Sent: Wednesday, March 25, 2015 7:59 AM To: bcooper@chla.usc.edu; histonet-bounces@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Pneumatic Tube Delivery System Hey Brian, We do use the tube system at our hospital. The rule here is that nothing in formalin is to be sent via tube. The specimen is considered irreplaceable and in the event of a malfunction in the system there is the possibility of specimen loss. Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From cecystan76 <@t> gmail.com Wed Mar 25 08:43:21 2015 From: cecystan76 <@t> gmail.com (Cecilia A.) Date: Wed Mar 25 08:43:24 2015 Subject: [Histonet] Phosphotungstic acid Message-ID: Hello Histonetters, I was wondering if anyone might be able to give some input regarding the use of Phosphotungstic acid in which we want to soak formalin fixed soft tissue for a few hours before it gets processed. Will subjecting it to PTA before processing affect its viability for further histology work (ie, H&E staining and IHC)? Thank you very much! C. From jkiernan <@t> uwo.ca Wed Mar 25 09:11:48 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Mar 25 09:11:57 2015 Subject: [Histonet] Phosphotungstic acid In-Reply-To: <7350854c32c72.5512c21d@uwo.ca> References: <7330ec01348d2.5512c0a9@uwo.ca> <72b0d8ff31f98.5512c0e5@uwo.ca> <72b0fb0237ce9.5512c123@uwo.ca> <7340bc09323c5.5512c161@uwo.ca> <7270ef4936d4c.5512c1a0@uwo.ca> <738095ab308c7.5512c1df@uwo.ca> <7350854c32c72.5512c21d@uwo.ca> Message-ID: <734083fd32307.55127bd4@uwo.ca> PTA can be used on tiny specimens to enhance electron-opacity. For light microscopy, PTA is used in the trichrome methods to enhance differential staining of collagen and cytoplasm, especially in paraffin sections. These uses of PTA are not the same as "soak formalin fixed soft tissue for a few hours before it gets processed". What are you hoping to achieve? John Kiernan London, Canada = = = On 25/03/15, "Cecilia A." wrote: > Hello Histonetters, > > I was wondering if anyone might be able to give some input regarding the > use of Phosphotungstic acid in which we want to soak formalin fixed soft > tissue for a few hours before it gets processed. Will subjecting it to PTA > before processing affect its viability for further histology work (ie, H&E > staining and IHC)? > > Thank you very much! > C. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From cjohnson <@t> nmda.nmsu.edu Wed Mar 25 09:19:55 2015 From: cjohnson <@t> nmda.nmsu.edu (Johnson, Carole) Date: Wed Mar 25 09:19:58 2015 Subject: [Histonet] Another Tol Blue question Message-ID: Good morning, So now I have a question about Tol Blue. Occasionally I will have a mast cell tumor that does not stain with Tol Blue (Tol Blue O, American MasterTech) but stains with a Giemsa (actually a DipQuik stain) and I am trying to find the reason for this. Any enlightenment is appreciated. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From Timothy.Morken <@t> ucsf.edu Wed Mar 25 10:40:35 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Wed Mar 25 10:41:44 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638632616514332@LRGHEXVS1.practice.lrgh.org> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D98638632616514332@LRGHEXVS1.practice.lrgh.org> Message-ID: <761E2B5697F795489C8710BCC72141FF367FF85F@ex07.net.ucsf.edu> Tom, I think every histotech does the best they can with the limited resources available. I don't blame anyone for lack of knowledge or skill due to their OJT because I came through the OJT route as well, after getting degrees in physiology and a two year certification in electron microscopy. I wound my way thru EM, histology, IHC and ended up working almost exclusively in IHC for many years and only recently came back to EM. Luckily I initially worked for a pathologist who supported the education of his techs and wanted "supertechs" who could do everything in histology. We had a group in the lab who studied together for the HT and HTL and we all learned a lot. However, what we did was still informal and certainly not comprehensive. Later on I worked in Saudi Arabia and had the chance to work with many other histotechs from other countries. Believe me, even with my degree and HTL I was outclassed by those other techs knowledge due to their formal education in the field. I was the one asking questions or being taught things I had no idea about. Things that had never come up in my necessarily limited OJT. But that is my point. The vast majority of histotechs fall into the field by accident, not by design, and whatever they learn is a circumstance of where they work, what that lab does and who is teaching them. I would never have thought of doing immunohistochemistry unless I had been hired to run an EM lab and, not having quite enough to keep me busy, been willing to help out in histology and learn everything there as well, just at the time IHC was coming into the lab, and a medical director willing to both teach and bring in people from outside to teach us. Someone else working somewhere else may not have that level of support at all. Indeed, I have met histotechs who tell me their medical directors will not even sign an ASCP application form for them to sit for an exam because if they figure if the tech gets the certification they will leave for a better job somewhere else! Compare that to a med tech who goes to college, finds out about the field of Medical Technology and has an entire department built around educating them to that end, along with hospitals that will provide internships for further training. That is far, far beyond what 99% of histotechs in the US ever get, if they get any formal training at all. Therefore, it is not reasonable to assume that just because someone learns a skill on the job and passes a basic test that they are equal in every way to someone with formal education, internships and good support throughout their education and training. It is only the very rare histotech who goes above and beyond the normal requirements of the job that achieves anything close to what med techs get as a normal course of events. So, I really appreciate what all good histotechs achieve with the near total lack of support, doing everything on their own, mostly due to their own desire to do well in a profession they love. Tim -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Wednesday, March 25, 2015 3:19 AM To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Tim for a Pathology Manager you seem to have a low opinion of the education and training of the Histo Techs that work for you. Is your training program accredited with one of the Histology schools or is your staff left to rend for themselves? By the way, the lab that I work at basis the starting salaries on your degree first, then specialty so MT, HT/HTL with BS degrees earn the same. Tom -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsf.edu] Sent: Tuesday, March 24, 2015 12:42 PM To: Podawiltz, Thomas; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Tom, no, Histo does start lower than med techs, but consider that a med tech has specialty training from the time they decide to go that route while most histotechs have general biology degrees and nothing but on the job training. Even with a certification a Histotech is not at the same level as a med tech simply due to the unstructured nature of their self-education and training. In 30+ years I have met only a handful of people who got any sort of degrees in Histotechnology, so waiting for those people to come along is not going to work for hiring. Most of our staff got their certification while working here and did it on their own. Only one has a degree in Histotechnology, and a BS at that!. A starting salary here is $36/hr and it is a $3 to $4 increase per level. The lab staff is unionized, and we compete with many large service labs (ie Kaiser) and many, many large biotech companies for the same pool of techs. Plus, it is expensive to live in the San Francisco Bay Area. We only recently (a few years ago) started this requirement in order to get our staff to a higher level. We still have staff without BA/BS degrees. The degree just needs to meet the requirements for certification so does not need to be a specialty degree. Tim -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Tuesday, March 24, 2015 9:06 AM To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology So just out of curiosity is the pay on the same level as that of a Med Tech with a BS? Does the BA/BS have to be in Histotechnology or is the BA/BS followed by one of the on-line certificate programs? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, March 24, 2015 11:47 AM To: Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Jennifer, we require a BA/BS degree for all Histotechnologist positions. However, in our 4 step categories Level 1 does not require certification, just the degree and the requirement that they get the certification within a year. Advancement to level 2 to 4 requires an HT or HTL certification (Level 1 = entry level bench tech, Level 2 is bench tech, level 3 is senior tech, level 4 is Lead tech). Supervisor requires and HTL. Considering that we already require a BA/BS degree for all levels, the fact a person has a HT or HTL is not going to matter much for levels 1 thru 4, only for supervisor level. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, March 23, 2015 7:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BS in Histotechnology In what areas would a facility hire an HTL over an HT? Is there a need for more HTL programs? 4 Thank you, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From jqb7 <@t> cdc.gov Wed Mar 25 10:56:33 2015 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Wed Mar 25 10:57:58 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <761E2B5697F795489C8710BCC72141FF367FF85F@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D98638632616514332@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF85F@ex07.net.ucsf.edu> Message-ID: <3B2CD438E1628A41BD687E98B963B78137F187DA@EMBX-CLFT4.cdc.gov> My abbreviated story: I went to college right out of high school...class of '74. During my sophomore year I spoke with my advisor and explained how much I loved biology/science....but did not want to be a nurse. She mentioned the lab sciences and had me visit a major hospital a couple hours away from where I was attending college to check things out. I saw chemistry, hematology, micro, blood banking, cytology and histology. I saw histology last and fell in love and knew instantly. I went back to my advisor and told her and she explained I didn't need a Bachelor's for that program so I decided to complete my sophomore and begin my year-long training. MANY years later I went back and finished my degree but I chose histology over many other options. I have not regretted it. Jeanine Sanders CDC Atlanta And for the record, I worked with Tim for years and he is an amazing histotech!!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, March 25, 2015 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Tom, I think every histotech does the best they can with the limited resources available. I don't blame anyone for lack of knowledge or skill due to their OJT because I came through the OJT route as well, after getting degrees in physiology and a two year certification in electron microscopy. I wound my way thru EM, histology, IHC and ended up working almost exclusively in IHC for many years and only recently came back to EM. Luckily I initially worked for a pathologist who supported the education of his techs and wanted "supertechs" who could do everything in histology. We had a group in the lab who studied together for the HT and HTL and we all learned a lot. However, what we did was still informal and certainly not comprehensive. Later on I worked in Saudi Arabia and had the chance to work with many other histotechs from other countries. Believe me, even with my degree and HTL I was outclassed by those other techs knowledge due to their formal education in the field. I was the one asking questions or being taught things I had no idea about. Things that had never come up in my necessarily limited OJT. But that is my point. The vast majority of histotechs fall into the field by accident, not by design, and whatever they learn is a circumstance of where they work, what that lab does and who is teaching them. I would never have thought of doing immunohistochemistry unless I had been hired to run an EM lab and, not having quite enough to keep me busy, been willing to help out in histology and learn everything there as well, just at the time IHC was coming into the lab, and a medical director willing to both teach and bring in people from outside to teach us. Someone else working somewhere else may not have that level of support at all. Indeed, I have met histotechs who tell me their medical directors will not even sign an ASCP application form for them to sit for an exam because if they figure if the tech gets the certification they will leave for a better job somewhere else! Compare that to a med tech who goes to college, finds out about the field of Medical Technology and has an entire department built around educating them to that end, along with hospitals that will provide internships for further training. That is far, far beyond what 99% of histotechs in the US ever get, if they get any formal training at all. Therefore, it is not reasonable to assume that just because someone learns a skill on the job and passes a basic test that they are equal in every way to someone with formal education, internships and good support throughout their education and training. It is only the very rare histotech who goes above and beyond the normal requirements of the job that achieves anything close to what med techs get as a normal course of events. So, I really appreciate what all good histotechs achieve with the near total lack of support, doing everything on their own, mostly due to their own desire to do well in a profession they love. Tim -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Wednesday, March 25, 2015 3:19 AM To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Tim for a Pathology Manager you seem to have a low opinion of the education and training of the Histo Techs that work for you. Is your training program accredited with one of the Histology schools or is your staff left to rend for themselves? By the way, the lab that I work at basis the starting salaries on your degree first, then specialty so MT, HT/HTL with BS degrees earn the same. Tom -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsf.edu] Sent: Tuesday, March 24, 2015 12:42 PM To: Podawiltz, Thomas; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Tom, no, Histo does start lower than med techs, but consider that a med tech has specialty training from the time they decide to go that route while most histotechs have general biology degrees and nothing but on the job training. Even with a certification a Histotech is not at the same level as a med tech simply due to the unstructured nature of their self-education and training. In 30+ years I have met only a handful of people who got any sort of degrees in Histotechnology, so waiting for those people to come along is not going to work for hiring. Most of our staff got their certification while working here and did it on their own. Only one has a degree in Histotechnology, and a BS at that!. A starting salary here is $36/hr and it is a $3 to $4 increase per level. The lab staff is unionized, and we compete with many large service labs (ie Kaiser) and many, many large biotech companies for the same pool of techs. Plus, it is expensive to live in the San Francisco Bay Area. We only recently (a few years ago) started this requirement in order to get our staff to a higher level. We still have staff without BA/BS degrees. The degree just needs to meet the requirements for certification so does not need to be a specialty degree. Tim -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Tuesday, March 24, 2015 9:06 AM To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology So just out of curiosity is the pay on the same level as that of a Med Tech with a BS? Does the BA/BS have to be in Histotechnology or is the BA/BS followed by one of the on-line certificate programs? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, March 24, 2015 11:47 AM To: Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Jennifer, we require a BA/BS degree for all Histotechnologist positions. However, in our 4 step categories Level 1 does not require certification, just the degree and the requirement that they get the certification within a year. Advancement to level 2 to 4 requires an HT or HTL certification (Level 1 = entry level bench tech, Level 2 is bench tech, level 3 is senior tech, level 4 is Lead tech). Supervisor requires and HTL. Considering that we already require a BA/BS degree for all levels, the fact a person has a HT or HTL is not going to matter much for levels 1 thru 4, only for supervisor level. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, March 23, 2015 7:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BS in Histotechnology In what areas would a facility hire an HTL over an HT? Is there a need for more HTL programs? 4 Thank you, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Wed Mar 25 11:09:16 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Wed Mar 25 11:12:16 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <38667E7FB77ECD4E91BFAEB8D98638632616514332@LRGHEXVS1.practice.lrgh.org> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D98638632616514332@LRGHEXVS1.practice.lrgh.org> Message-ID: <761E2B5697F795489C8710BCC72141FF367FF93A@ex07.net.ucsf.edu> Tom, no we don't have a program here. In fact the only one on the west coast is in southern California (we did try to work with the SF city college to start a program but their funding fell through). Another local college is trying to start a course and I'm getting involved with that. For the time being we just try to find the best candidates we can. I sent a much longer response to histonet, but generally histotechs are, as you well know, a grab bag of various levels of training and knowledge. Only one in our lab has a BS degree in Histotechnology (from Ohio). Nothing against histotechs at all, as I am an OJT histotech but formally -rained EM tech. People do the best they can with their limited exposure to the field. In my long experience in the several labs I have worked in, I have only seen a few that have risen to the level of a med tech. NSH meetings are a different story - mostly people who have taken the time and made the extraordinary effort to educate themselves to a high level. While working oversees my eyes were opened to what other techs in other countries do to educate and supply histotechs. The US is way, way at the low end of the scale in that regard. Most other countries have degreed or technical programs specifically for lab techs of all kinds. In most countries Histotechnology is a specialty of Medical Technology and only done after a full med tech degree is earned - another year of school for the specialization. I really can't look at the situation in the US and say it is ok after seeing what goes on elsewhere. Tim -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Wednesday, March 25, 2015 3:19 AM To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Tim for a Pathology Manager you seem to have a low opinion of the education and training of the Histo Techs that work for you. Is your training program accredited with one of the Histology schools or is your staff left to rend for themselves? By the way, the lab that I work at basis the starting salaries on your degree first, then specialty so MT, HT/HTL with BS degrees earn the same. Tom -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsf.edu] Sent: Tuesday, March 24, 2015 12:42 PM To: Podawiltz, Thomas; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Tom, no, Histo does start lower than med techs, but consider that a med tech has specialty training from the time they decide to go that route while most histotechs have general biology degrees and nothing but on the job training. Even with a certification a Histotech is not at the same level as a med tech simply due to the unstructured nature of their self-education and training. In 30+ years I have met only a handful of people who got any sort of degrees in Histotechnology, so waiting for those people to come along is not going to work for hiring. Most of our staff got their certification while working here and did it on their own. Only one has a degree in Histotechnology, and a BS at that!. A starting salary here is $36/hr and it is a $3 to $4 increase per level. The lab staff is unionized, and we compete with many large service labs (ie Kaiser) and many, many large biotech companies for the same pool of techs. Plus, it is expensive to live in the San Francisco Bay Area. We only recently (a few years ago) started this requirement in order to get our staff to a higher level. We still have staff without BA/BS degrees. The degree just needs to meet the requirements for certification so does not need to be a specialty degree. Tim -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Tuesday, March 24, 2015 9:06 AM To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology So just out of curiosity is the pay on the same level as that of a Med Tech with a BS? Does the BA/BS have to be in Histotechnology or is the BA/BS followed by one of the on-line certificate programs? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, March 24, 2015 11:47 AM To: Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Jennifer, we require a BA/BS degree for all Histotechnologist positions. However, in our 4 step categories Level 1 does not require certification, just the degree and the requirement that they get the certification within a year. Advancement to level 2 to 4 requires an HT or HTL certification (Level 1 = entry level bench tech, Level 2 is bench tech, level 3 is senior tech, level 4 is Lead tech). Supervisor requires and HTL. Considering that we already require a BA/BS degree for all levels, the fact a person has a HT or HTL is not going to matter much for levels 1 thru 4, only for supervisor level. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, March 23, 2015 7:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BS in Histotechnology In what areas would a facility hire an HTL over an HT? Is there a need for more HTL programs? 4 Thank you, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From abadesuyi <@t> nrh-ok.com Wed Mar 25 11:25:31 2015 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Wed Mar 25 11:25:38 2015 Subject: [Histonet] Cutting of Paraffin Blocks Message-ID: <04EE4F75BB5FB246ADB68D69B746044399095FC4DD@MAIL.nrhnt.nrh-ok.com> Hi, How are you guys doing? I hope you are all doing well. Please I have two histology trainees and they have spent more than six months in the histology lab and they cannot cut blocks yet. They are comfortable with filing slides and blocks. But I have been telling them that they have to start cutting now. My question now is am I too hard on them? Best regards, Adesupo Adesuyi ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From suetp918 <@t> comcast.net Wed Mar 25 11:36:42 2015 From: suetp918 <@t> comcast.net (suetp918) Date: Wed Mar 25 11:36:59 2015 Subject: [Histonet] Cutting of Paraffin Blocks Message-ID: U r absolutely not 2 gard they should be cutting my the first month. ?Filing is nor a histo function but a collateral dutyand will not help them as histotechs. Just my thought Sent from my Verizon Wireless 4G LTE smartphone -------- Original message -------- From: "Adesupo, Adesuyi (Banjo)" Date:03/25/2015 12:25 PM (GMT-05:00) To: "'histonet@lists.utsouthwestern.edu'" Subject: [Histonet] Cutting of Paraffin Blocks Hi, How are you guys doing? I hope you are all doing well. Please I have two histology trainees and they have spent more than six months in the histology lab and they cannot cut blocks yet. They are comfortable with filing slides and blocks. But I have been telling them that they have to start cutting now. My question now is am I too hard on them? Best regards, Adesupo Adesuyi ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert <@t> pathmdlabs.com Wed Mar 25 11:48:41 2015 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Wed Mar 25 11:48:45 2015 Subject: [Histonet] RE: Cutting of Paraffin Blocks In-Reply-To: <04EE4F75BB5FB246ADB68D69B746044399095FC4DD@MAIL.nrhnt.nrh-ok.com> References: <04EE4F75BB5FB246ADB68D69B746044399095FC4DD@MAIL.nrhnt.nrh-ok.com> Message-ID: <12ECD7346266D74691EC2BFC75285E455B24DB65@BFL323E10.pathmdlabs.local> Heck no, crack that whip! Give them practice tissue that isn't important so they won't be afraid of ruining something. Laurie Colbert, HT (ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Wednesday, March 25, 2015 9:26 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cutting of Paraffin Blocks Hi, How are you guys doing? I hope you are all doing well. Please I have two histology trainees and they have spent more than six months in the histology lab and they cannot cut blocks yet. They are comfortable with filing slides and blocks. But I have been telling them that they have to start cutting now. My question now is am I too hard on them? Best regards, Adesupo Adesuyi ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock <@t> mclean.harvard.edu Wed Mar 25 11:48:53 2015 From: twheelock <@t> mclean.harvard.edu (Wheelock, Timothy R.) Date: Wed Mar 25 11:48:58 2015 Subject: [Histonet] Cutting of Paraffin blocks Message-ID: <69718C0B0B3C414D9F8E7214AD400CC9774EFC60@PHSX10MB11.partners.org> Hi Adesupo: In 1995, I had two 18 year old summer students from Strasborg, France. They were here in our neuropathology lab for 2 months. I had them cutting within a week or so after their arrival. By the end of the 2 months, they were processing, embedding, trimming, sectioning, and staining beautiful sections. All they needed was a careful safety orientation, a demonstration, and then practice. Hope this helps, Tim Tim Wheelock Harvard Brain Bank Mclean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From anna.coffey <@t> nih.gov Wed Mar 25 11:52:36 2015 From: anna.coffey <@t> nih.gov (Coffey, Anna (NIH/NCI) [C]) Date: Wed Mar 25 11:52:54 2015 Subject: [Histonet] RE: BS in Histotechnology Message-ID: <5C3E10119A1B824FBE92B08279F74A910178D637@msgb10.nih.gov> As a newly certified tech, I've got to say that I really appreciate this conversation and hearing others' stories. I am someone who did sort of fall into histology by accident; I learned processing, sectioning, and H&E staining while preparing the samples for my MS thesis. I had a BS in Biology and a MS in Marine Science and found very few job opportunities available upon graduation. I knew I had a strong interest in pathology and luckily had learned a skill (histology) to get a job in a histopath lab. I was encouraged to get my certification, but when I started looking into it, I was a bit frustrated by what seemed like a very informal process and severe lack of resources. It took me a very long time to figure out if I was even eligible to sit for the exam! I was so grateful to find out about this listserve and everyone was very helpful and supportive throughout the whole exam prep process. However, there were many times I felt I had nowhere to turn for answers. I will admit that I underestimated the amount and depth of questions on the exam and was shocked by the amount of time it took to prepare myself. I basically taught myself everything from books and online test prep materials. This left me feeling like, although I had learned A TON, there were still significant holes in my knowledge and I have since resigned myself to trying to pick up as much as I can from any resources I can get my hands on. I certainly don't want to imply that my experience is the norm, but I do wish there was more information out there about histology as a career. I often feel like I'm trying to piece together a niche career because of the way I came into the field and I don't know many other certified techs that I can try to model my own career path after. I do consider this listserve a very valuable resource and greatly appreciate everyone's contributions. Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey@nih.gov 301-846-1730 From azdudley <@t> hotmail.com Wed Mar 25 11:57:09 2015 From: azdudley <@t> hotmail.com (anita) Date: Wed Mar 25 11:57:12 2015 Subject: [Histonet] sta on Message-ID: how many people use sta on in their waterbath? we have not for years, but have a new person who wants me to get some, she is having trouble with the ribbons floating away. thanks for your input, anita dudley providence hospital mobile, al From suetp918 <@t> comcast.net Wed Mar 25 12:07:49 2015 From: suetp918 <@t> comcast.net (suetp918) Date: Wed Mar 25 12:08:05 2015 Subject: [Histonet] sta on Message-ID: Not good 4 ihc Sent from my Verizon Wireless 4G LTE smartphone -------- Original message -------- From: anita Date:03/25/2015 12:57 PM (GMT-05:00) To: "Histonet@lists.utsouthwestern.edu" Subject: [Histonet] sta on how many people use sta on in their waterbath? we have not for years, but have a new person who wants me to get some, she is having trouble with the ribbons floating away. thanks for your input, anita dudley providence hospital mobile, al _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Wed Mar 25 12:13:42 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Mar 25 12:13:48 2015 Subject: [Histonet] sta on In-Reply-To: References: Message-ID: <838708e7f75c494b93a07c46b8f16bca@MAIL13M2N2.ad.uams.edu> Not allowed in our lab at all. We use charged slides for everything since we also cut IHC slides. It is not good for IHC and can cause hideous background on slides. We rarely have anything float. Pam Marcum -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita Sent: Wednesday, March 25, 2015 11:57 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] sta on how many people use sta on in their waterbath? we have not for years, but have a new person who wants me to get some, she is having trouble with the ribbons floating away. thanks for your input, anita dudley providence hospital mobile, al _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Cnituda <@t> nvdermatology.com Wed Mar 25 11:42:43 2015 From: Cnituda <@t> nvdermatology.com (Carl Nituda) Date: Wed Mar 25 12:14:46 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <761E2B5697F795489C8710BCC72141FF367FF93A@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D98638632616514332@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF93A@ex07.net.ucsf.edu> Message-ID: Best program and training to go to is at Barry University in Miami Shores, Florida. I'm not in any part of the faculty; I'm only an alumni c/o 2008. The degree is a BS in Clinical Biology with specialization in Histotechnology. The mean GPA for all graduates in the program is 3.42 and the faculty are amazing group of people. The courses includes Parasitology, BioChem 1 & 2, Immunology, Serology, Micro, Clin. Chemistry, Lab Management, Medical Ethics, & etc. This Catholic University also has opportunities for volunteerism in surrounding neighborhood and abroad to expose students to diversity and adversity. Go to www.barry.edu for more information. Carl Nituda, BS, HTL(ASCP) QIHC www.Nituda.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, March 25, 2015 9:09 AM To: Podawiltz, Thomas; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Tom, no we don't have a program here. In fact the only one on the west coast is in southern California (we did try to work with the SF city college to start a program but their funding fell through). Another local college is trying to start a course and I'm getting involved with that. For the time being we just try to find the best candidates we can. I sent a much longer response to histonet, but generally histotechs are, as you well know, a grab bag of various levels of training and knowledge. Only one in our lab has a BS degree in Histotechnology (from Ohio). Nothing against histotechs at all, as I am an OJT histotech but formally -rained EM tech. People do the best they can with their limited exposure to the field. In my long experience in the several labs I have worked in, I have only seen a few that have risen to the level of a med tech. NSH meetings are a different story - mostly people who have taken the time and made the extraordinary effort to educate themselves to a high level. While working oversees my eyes were opened to what other techs in other countries do to educate and supply histotechs. The US is way, way at the low end of the scale in that regard. Most other countries have degreed or technical programs specifically for lab techs of all kinds. In most countries Histotechnology is a specialty of Medical Technology and only done after a full med tech degree is earned - another year of school for the specialization. I really can't look at the situation in the US and say it is ok after seeing what goes on elsewhere. Tim -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Wednesday, March 25, 2015 3:19 AM To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Tim for a Pathology Manager you seem to have a low opinion of the education and training of the Histo Techs that work for you. Is your training program accredited with one of the Histology schools or is your staff left to rend for themselves? By the way, the lab that I work at basis the starting salaries on your degree first, then specialty so MT, HT/HTL with BS degrees earn the same. Tom -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsf.edu] Sent: Tuesday, March 24, 2015 12:42 PM To: Podawiltz, Thomas; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Tom, no, Histo does start lower than med techs, but consider that a med tech has specialty training from the time they decide to go that route while most histotechs have general biology degrees and nothing but on the job training. Even with a certification a Histotech is not at the same level as a med tech simply due to the unstructured nature of their self-education and training. In 30+ years I have met only a handful of people who got any sort of degrees in Histotechnology, so waiting for those people to come along is not going to work for hiring. Most of our staff got their certification while working here and did it on their own. Only one has a degree in Histotechnology, and a BS at that!. A starting salary here is $36/hr and it is a $3 to $4 increase per level. The lab staff is unionized, and we compete with many large service labs (ie Kaiser) and many, many large biotech companies for the same pool of techs. Plus, it is expensive to live in the San Francisco Bay Area. We only recently (a few years ago) started this requirement in order to get our staff to a higher level. We still have staff without BA/BS degrees. The degree just needs to meet the requirements for certification so does not need to be a specialty degree. Tim -----Original Message----- From: Podawiltz, Thomas [mailto:tpodawiltz@lrgh.org] Sent: Tuesday, March 24, 2015 9:06 AM To: Morken, Timothy; Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology So just out of curiosity is the pay on the same level as that of a Med Tech with a BS? Does the BA/BS have to be in Histotechnology or is the BA/BS followed by one of the on-line certificate programs? Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, March 24, 2015 11:47 AM To: Jennifer MacDonald; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BS in Histotechnology Jennifer, we require a BA/BS degree for all Histotechnologist positions. However, in our 4 step categories Level 1 does not require certification, just the degree and the requirement that they get the certification within a year. Advancement to level 2 to 4 requires an HT or HTL certification (Level 1 = entry level bench tech, Level 2 is bench tech, level 3 is senior tech, level 4 is Lead tech). Supervisor requires and HTL. Considering that we already require a BA/BS degree for all levels, the fact a person has a HT or HTL is not going to matter much for levels 1 thru 4, only for supervisor level. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Monday, March 23, 2015 7:52 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BS in Histotechnology In what areas would a facility hire an HTL over an HT? Is there a need for more HTL programs? 4 Thank you, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper <@t> chla.usc.edu Wed Mar 25 12:17:52 2015 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Wed Mar 25 12:17:57 2015 Subject: [Histonet] RE: Cutting of Paraffin Blocks In-Reply-To: <12ECD7346266D74691EC2BFC75285E455B24DB65@BFL323E10.pathmdlabs.local> References: <04EE4F75BB5FB246ADB68D69B746044399095FC4DD@MAIL.nrhnt.nrh-ok.com> <12ECD7346266D74691EC2BFC75285E455B24DB65@BFL323E10.pathmdlabs.local> Message-ID: Completely agree. Give them practice tissue blocks because it's expected that they WILL destroy them during the early stages of their learning process. If I can go one further here--when these students have demonstrated acceptable microtomy technique (and when you feel comfortable), be sure to teach them how to realign their microtome's orientation head/specimen clamp to the surface of blocks that have been previously cut. I cannot tell you how many histotechs I've met in my career who didn't possess this skill and simply "refaced" these blocks. One even yelled at the Biomedical Maintenance tech when he serviced her microtome and readjusted the orientation! I have a student in our lab right now, and tasked her with providing me 5 H&E's from each of the practice blocks I gave her. Here's the catch. In between each section that she collects, she is supposed to completely misalign the specimen head, then realign to the surface of the block. I told her that all five H&E's from each block should look relatively similar by the time she's done, and that there should be no appreciable tissue loss. While this might seem like a torture test for beginners, it will only serve them (and ultimately the patients they serve) so well in the future. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357???? Pager: 213-209-0184 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, March 25, 2015 9:49 AM To: Adesupo, Adesuyi (Banjo); 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Cutting of Paraffin Blocks Heck no, crack that whip! Give them practice tissue that isn't important so they won't be afraid of ruining something. Laurie Colbert, HT (ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Wednesday, March 25, 2015 9:26 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cutting of Paraffin Blocks Hi, How are you guys doing? I hope you are all doing well. Please I have two histology trainees and they have spent more than six months in the histology lab and they cannot cut blocks yet. They are comfortable with filing slides and blocks. But I have been telling them that they have to start cutting now. My question now is am I too hard on them? Best regards, Adesupo Adesuyi ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From epeters2 <@t> gmu.edu Wed Mar 25 12:43:58 2015 From: epeters2 <@t> gmu.edu (Esther C Peters) Date: Wed Mar 25 12:44:04 2015 Subject: [Histonet] Re: Cutting of Paraffin Blocks In-Reply-To: References: <04EE4F75BB5FB246ADB68D69B746044399095FC4DD@MAIL.nrhnt.nrh-ok.com> <12ECD7346266D74691EC2BFC75285E455B24DB65@BFL323E10.pathmdlabs.local>, Message-ID: <1427305436959.73818@gmu.edu> I start my students with plain paraffin blocks (no tissue in them) that they make themselves, to start. After they have worked with those, looking at how adjustments, then icing, then moving sections to the waterbath work, then I give them old practice blocks. This seems to be less intimidating than starting with tissue (although I think that is how I learned!). Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epeters2@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cooper, Brian Sent: Wednesday, March 25, 2015 1:17 PM To: Laurie Colbert; Adesupo, Adesuyi (Banjo); 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Cutting of Paraffin Blocks Completely agree. Give them practice tissue blocks because it's expected that they WILL destroy them during the early stages of their learning process. If I can go one further here--when these students have demonstrated acceptable microtomy technique (and when you feel comfortable), be sure to teach them how to realign their microtome's orientation head/specimen clamp to the surface of blocks that have been previously cut. I cannot tell you how many histotechs I've met in my career who didn't possess this skill and simply "refaced" these blocks. One even yelled at the Biomedical Maintenance tech when he serviced her microtome and readjusted the orientation! I have a student in our lab right now, and tasked her with providing me 5 H&E's from each of the practice blocks I gave her. Here's the catch. In between each section that she collects, she is supposed to completely misalign the specimen head, then realign to the surface of the block. I told her that all five H&E's from each block should look relatively similar by the time she's done, and that there should be no appreciable tissue loss. While this might seem like a torture test for beginners, it will only serve them (and ultimately the patients they serve) so well in the future. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, March 25, 2015 9:49 AM To: Adesupo, Adesuyi (Banjo); 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Cutting of Paraffin Blocks Heck no, crack that whip! Give them practice tissue that isn't important so they won't be afraid of ruining something. Laurie Colbert, HT (ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Adesupo, Adesuyi (Banjo) Sent: Wednesday, March 25, 2015 9:26 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Cutting of Paraffin Blocks Hi, How are you guys doing? I hope you are all doing well. Please I have two histology trainees and they have spent more than six months in the histology lab and they cannot cut blocks yet. They are comfortable with filing slides and blocks. But I have been telling them that they have to start cutting now. My question now is am I too hard on them? Best regards, Adesupo Adesuyi ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pmcdavid <@t> mhg.com Wed Mar 25 12:46:28 2015 From: Pmcdavid <@t> mhg.com (Patti McDavid) Date: Wed Mar 25 12:47:05 2015 Subject: [Histonet] RE: Pneumatic Tube Delivery System Message-ID: <1F886A89F4A53345928A11203C028957EC4C8B4B@JANDC600-MBN01.mhg.local> RE: Pneumatic Tube Delivery System We only send specimens that are easily obtained through the tube system as well. Most commonly blood or urine. We do not send tissue for pathology fresh or containing formalin through the tube system. A formalin spill would contaminate the tube system and require proper clean up. Patti L. McDavid Laboratory Manager 4500 Thirteenth Street P.O. Box 1810 Gulfport, MS 39502-1810 Phone 228-575-2340 Fax 228-865-3325 Pmcdavid@mhg.com [http://www.gulfportmemorial.com/images/14MH81-BESTE_SIG-RANKED-A-BESTD2.GIF] http://health.usnews.com/best-hospitals/area/ms ________________________________ This email may contain information covered under the Mississippi Privacy Law (Miss. Code Ann. ? 75-24-29), the Privacy Act of 1974 (5 U.S.C. ? 552a) and/or the Health Insurance Portability and Accountability Act of 1996 (Pub. L. No. 104-191) and its accompanying regulations. Healthcare information is personal and sensitive and must be protected in accordance with these provisions. If this email contains healthcare information, it is being disclosed to you only after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Re-disclosure without additional patient authorization, unless otherwise permitted by law, is prohibited. **********PRIVATE AND CONFIDENTIAL********** If you are not the intended recipient of this email, be advised that any use, disclosure, copying, distribution or taking any action in reliance on the contents of the information contained therein is strictly prohibited. If you have received this email in error, please contact the sender immediately by reply email and then destroy/delete all copies of the original message and any attachment(s) thereto. From TNMayer <@t> mdanderson.org Wed Mar 25 13:08:01 2015 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Mar 25 13:08:06 2015 Subject: [Histonet] RE: BS in Histotechnology Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881D5369F0@D1PWPEXMBX05.mdanderson.edu> Like many of us, I stumbled I into Histo. I took a class in Histology as part of a Pre-Med curriculum in college and liked what I saw. We were never told that it was a career field, so a few years later when I was offered the chance to work in a histo lab I took it. Since I had a BS, I was encouraged to take the HT exam, but didn't do IHC so not the HTL. Years later I was told that I could get a salary increase with the HTL. Being in education, I see the need for more HTL's, but without a salary difference it is hard to sell. The new healthcare law, may be able to help us, in regards to the need for mid-level providers. The fact that HTL's can do more complicated testing helps. Employers are beginning to require certification, but experience goes a long way. In some places people have had to change their title, because they are not certified. Sometimes it seems that in regards to histo the chicken/egg question comes up. Without the differences in salaries, it is hard to attract degreed and certified techs, but without the certified techs it is hard to get better salaries. I agree, NSH needs to do more, but so does ASCP. We need the respect and recognition that is deserved. Histotechnology is an obscure profession that few people, let alone those in healthcare, are aware of. Everybody has seen our work, but doesn't really understand the effort that we put forth. Education institutions should promote their programs (mine included), and actively recruit students. No one really knows that education programs exist for the field. One thing I mention to potential applicants is how many jobs I held at once, just because they were there. CAP should have a link to the NSH website to encourage education, working techs should have more pride in their craft and push education and certification. Those of us who took the OJT route need to keep abreast of the changes in the field and participate in education opportunities. There should be no room for jealousy and back biting. Many techs who are certified, did not keep it current because they did not plan to leave their institution, and now regret it. Education and certification are essential for the continued progression of our field, but as a whole we have to decide what we want and come together to accomplish it. HT is important, HTL is important. Not everyone is cut out to get a four year degree, but each person who is employed as a histotech should be certified for the betterment of the field as a whole. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 -------- Original message -------- From: Bernice Frederick Date:03/24/2015 3:27 PM (GMT-05:00) To: "Sanders, Jeanine (CDC/OID/NCEZID)" , Carl Nituda , "Marcum, Pamela A" , Sue , Timothy Morken Cc: histonet@lists.utsouthwestern.edu, Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology "They" don't realize the theory we have to learn and those questions we have to answer like " What's the best fixative for a pheochromocytoma?" You tell them and they say the pathologist says B-5, to which I said, well they wouldn't pass out registry exam with that answer.....Grrr. Or the difference between a Mucin, Pas and Alcian Blue. The cytopath who asked did really need to know. As well I vaguely recall a question back on my HTL exam asking why a pathologist would request a mucin stain.... Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, March 24, 2015 2:14 PM To: Carl Nituda; Marcum, Pamela A; Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I know someone personally that works in a hospital and it hast Histotechnologist by his name....and he never took the HTL exam. He said his hospital bases it on experience ________________________________________ From: Carl Nituda [Cnituda@nvdermatology.com] Sent: Tuesday, March 24, 2015 2:32 PM To: Marcum, Pamela A; Sanders, Jeanine (CDC/OID/NCEZID); Sue; Timothy Morken Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald Subject: RE: [Histonet] BS in Histotechnology I personally think that a person just can't call themselves a Histotechnologist unless they went to school, training, and then pass the BOC by ASCP. Anyone, I mean anyone can perform a job with proper training in any field but that doesn't mean they should have that title until they pass certification. For hiring managers, I encourage you to hire certified candidates as priority and call them a Histotechnician, or Histotechnologist based on their certification. If a person is doing Histology work and is uncertified, encourage them to be certified and just don't give them a title. Imagine a world when people doing the job is actually certified like other professions, then you will get the respect from your colleagues that you deserve. Changes for the future of the profession starts with good leaders. Have a good and blessed week everyone. Carl Nituda From POWELL_SA <@t> mercer.edu Wed Mar 25 14:04:05 2015 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Wed Mar 25 14:03:43 2015 Subject: [Histonet] sta on In-Reply-To: References: Message-ID: <9BF995BC0E47744E9673A41486E24EE25C020723B5@MERCERMAIL.MercerU.local> I use it. Back when I was doing my IHC reference lab, I had certain tissues that would not stay on with charged slides. These were bloody, crunchy and brittle tissues mostly. When they washed, I used Sta-On and they worked great. No washing. After my institution closed the IHC part of my lab in 2002, I decided to use Sta-On and not charged slides which was more cost effective. I still use it today. Very seldom do I lose any sections. Shirley Powell MUSM -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita Sent: Wednesday, March 25, 2015 12:57 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] sta on how many people use sta on in their waterbath? we have not for years, but have a new person who wants me to get some, she is having trouble with the ribbons floating away. thanks for your input, anita dudley providence hospital mobile, al _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From billodonnell <@t> catholichealth.net Wed Mar 25 14:46:38 2015 From: billodonnell <@t> catholichealth.net (O'Donnell, Bill) Date: Wed Mar 25 14:46:46 2015 Subject: [Histonet] sta on In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25C020723B5@MERCERMAIL.MercerU.local> References: <9BF995BC0E47744E9673A41486E24EE25C020723B5@MERCERMAIL.MercerU.local> Message-ID: We use it and are happy with it. Measure out your dose and you will not have background. Never lose sections. We do not do IHC :( It seems a reasonable request and a bottle should last a while at a capful a day. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley A. Powell Sent: Wednesday, March 25, 2015 2:04 PM To: anita; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] sta on I use it. Back when I was doing my IHC reference lab, I had certain tissues that would not stay on with charged slides. These were bloody, crunchy and brittle tissues mostly. When they washed, I used Sta-On and they worked great. No washing. After my institution closed the IHC part of my lab in 2002, I decided to use Sta-On and not charged slides which was more cost effective. I still use it today. Very seldom do I lose any sections. Shirley Powell MUSM -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of anita Sent: Wednesday, March 25, 2015 12:57 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] sta on how many people use sta on in their waterbath? we have not for years, but have a new person who wants me to get some, she is having trouble with the ribbons floating away. thanks for your input, anita dudley providence hospital mobile, al _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=3iEA9oByFKGUVmHHjEGViq9-hi91LvAPvOKSxbQaAak&m=kpqmYmTQTj1xDXohwaN9x8KK8weBlxq-xBEsxumqaxQ&s=dmbAiCkj5VV6-2bEUoWm7VD95eY63lwV_bYms69KZG0&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=3iEA9oByFKGUVmHHjEGViq9-hi91LvAPvOKSxbQaAak&m=kpqmYmTQTj1xDXohwaN9x8KK8weBlxq-xBEsxumqaxQ&s=dmbAiCkj5VV6-2bEUoWm7VD95eY63lwV_bYms69KZG0&e= This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From cecystan76 <@t> gmail.com Wed Mar 25 18:22:01 2015 From: cecystan76 <@t> gmail.com (Cecilia A.) Date: Wed Mar 25 18:22:02 2015 Subject: Fwd: [Histonet] Phosphotungstic acid References: Message-ID: <094295B4-606F-432C-BACF-F3AA510DA20A@gmail.com> > > Hi John, > > Thank you for your reply. > > In a Developmental Dynamics paper in 2009, PTA was used as a "simple staining method" that produced overall contrast for X-ray imaging soft tissues similar to how Osmium Tetroxide was also used as a soak for this purpose. > > We were interested in using PTA as it doesn't render the soft tissue black like Osmium Tetroxide does, so that there's still a chance we would be able to do further histology work on the tissue. However, we wonder what PTA might do to the fixed yet unprocessed tissue when it is soaked for this imaging purpose, and if it might affect its viability for staining and IHC work. > > Thank you, > C. > >> On Wed, Mar 25, 2015 at 9:11 AM, John Kiernan wrote: >> PTA can be used on tiny specimens to enhance electron-opacity. For light microscopy, PTA is used in the trichrome methods to enhance differential staining of collagen and cytoplasm, especially in paraffin sections. These uses of PTA are not the same as "soak formalin fixed soft tissue for a few hours before it gets processed". What are you hoping to achieve? >> >> John Kiernan >> London, Canada >> = = = >> >>> On 25/03/15, "Cecilia A." wrote: >>> Hello Histonetters, >>> >>> I was wondering if anyone might be able to give some input regarding the >>> use of Phosphotungstic acid in which we want to soak formalin fixed soft >>> tissue for a few hours before it gets processed. Will subjecting it to PTA >>> before processing affect its viability for further histology work (ie, H&E >>> staining and IHC)? >>> >>> Thank you very much! >>> C. >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From KSimeone <@t> leavittmgt.com Thu Mar 26 11:30:35 2015 From: KSimeone <@t> leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Thu Mar 26 11:30:39 2015 Subject: [Histonet] FULL TIME POSITION DELRAY BEACH, FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C37C8F486@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Dermatology Lab. This is a permanent full time SECOND SHIFT (40 hours) position with benefits (medical/401k/vacation) and competitive pay. THIS IS A DRUG FREE WORKPLACE. ONLY SERIOUS INQURIES, please read EVERY qualification desired. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengimann@leavittmgt.com if interested. *Full time position Mon-Fri 12:30p-9p (general tech duties to include grossing, microtomy, embedding, etc) *MUST be licensed as a FL histotechnologist or technician *will train but you MUST be open and willing to learn and absorb the routine quickly *must be self motivated, reliable and a team player Kari M Simeone 561.819.6517 fax ksimeone@leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From gayle.callis <@t> bresnan.net Thu Mar 26 12:19:17 2015 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Mar 26 12:19:26 2015 Subject: [Histonet] Staining dishes and slide racks for sale Message-ID: <001d01d067e8$fdd988f0$f98c9ad0$@bresnan.net> Dear Histonetters, Janet Maass has these new and used staining dishes, covers and slide racks for sale. I will be happy to forward your email message to her since Histonet messes with email addresses. 22 glass staining dishes with covers (for 50 slides) 4 extra covers available 8 glass staining dishes with covers (for 30 slides) 6 extra dishes available 12 new glass staining dishes still in original boxes (for 30 slides) 10 large (50 slide capacity) metal staining racks with 16 handles 4 small (30 slide capacity)metal staining racks with 3 handles Gayle M. Callis HTL/HT/MT(ASCP) From Timothy.Morken <@t> ucsf.edu Thu Mar 26 12:30:42 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Thu Mar 26 12:30:51 2015 Subject: [Histonet] for free...Numbered, lettered beads for embedding ID Message-ID: <761E2B5697F795489C8710BCC72141FF367FFE2C@ex07.net.ucsf.edu> Free for anyone: I have tons of left over white numbered beads that we used for embedding ID before we went to barcoding. Numbered/lettered 0-9, A-E, various quantities but generally many hundred of each. Any takers? I'll ship. We got these from Cancer Diagnostics: http://www.cancerdiagnostics.com/CDI_Products.aspx?pid=109 Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken@ucsfmedctr.org From shultz11 <@t> cox.net Thu Mar 26 13:41:01 2015 From: shultz11 <@t> cox.net (shultz11) Date: Thu Mar 26 13:41:10 2015 Subject: [Histonet] Job Message-ID: <8aquablyvibu7lv6wcq2tipv.1427395261864@email.android.com> Histology technician job at LSU in Baton Rouge, LA. Go to lsu.edu and look for research associate 2. Sent from my Verizon Wireless 4G LTE smartphone From TanyaAbbott <@t> catholichealth.net Thu Mar 26 14:24:15 2015 From: TanyaAbbott <@t> catholichealth.net (Abbott, Tanya) Date: Thu Mar 26 14:24:19 2015 Subject: [Histonet] Pipettes Message-ID: <852F7D2C14FB464D80E182B15DB138AF4CDDDD89@CHIEX005.CHI.catholichealth.net> CAP checklist ANP.23085 refers to Pipettes "that are used for quantitative dispensing of material are checked for accuracy and reproducibility at least annually, and results documented." We perform our Special Stains by hand. So we use disposable, glass, Non Class A pipettes. About 15 in a bag. We obviously do not check these one by one for reproducibility. I did call CAP and although he tried to be helpful, I still am confused! Any suggestions on how anyone else handles this? Thanks in advance, Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From GoetzF <@t> si.edu Thu Mar 26 14:28:44 2015 From: GoetzF <@t> si.edu (Goetz, Freya E.) Date: Thu Mar 26 14:28:51 2015 Subject: [Histonet] Ted Pella Embedding Center Message-ID: <0696DF79-3761-496A-841A-725300BA6C95@si.edu> Hello! I was wondering if anyone has experience, anecdotal or first hand with the Ted Pella embedding center: http://www.tedpella.com/histo_html/histo1.htm.aspx#No-28157 . They don?t offer demo machines. We are looking for something in this price range that is very simple. We don?t need fancy buttons or settings, just an embedding center that can turn on, set to a specific temperature and where the cooling unit is separate from the heating unit. I am skeptical of any embedding center that comes at that price new and with no demo option so I am turning to the wise community. Any opinions welcome. Thank you! Freya From Timothy.Morken <@t> ucsf.edu Thu Mar 26 14:31:26 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Thu Mar 26 14:31:37 2015 Subject: [Histonet] RE: Pipettes In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF4CDDDD89@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF4CDDDD89@CHIEX005.CHI.catholichealth.net> Message-ID: <761E2B5697F795489C8710BCC72141FF367FFEC0@ex07.net.ucsf.edu> Disposable pipets are exempt from this. I believe it means pipeters that use disposable tips, which we calibrate annually through an outside company (though you can do it yourself if you have an analytical balance). If you really have an issue with this then put into your procedures a tolerance for measuring liquids that matches that of the disposable pipets (ie, +/- 0.1ml per 10ml or something like that) Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Thursday, March 26, 2015 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pipettes CAP checklist ANP.23085 refers to Pipettes "that are used for quantitative dispensing of material are checked for accuracy and reproducibility at least annually, and results documented." We perform our Special Stains by hand. So we use disposable, glass, Non Class A pipettes. About 15 in a bag. We obviously do not check these one by one for reproducibility. I did call CAP and although he tried to be helpful, I still am confused! Any suggestions on how anyone else handles this? Thanks in advance, Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jvickroy <@t> SpringfieldClinic.com Thu Mar 26 16:04:15 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Thu Mar 26 16:04:21 2015 Subject: [Histonet] Xylene spills Message-ID: <9B1A1501A800064397369BD8072E6BCAB46DFE@E2K10DB.springfieldclinic.com> We are setting up a histology lab in a clinic. We use very little xylene and only in the automated coverslipper. I am writing up a procedure to handle a xylene spill. When I was at the hospital I segregated small and large spills with separate procedures, both however involved the use of a PAPR. I am looking for an economical way to clean up a potential xylene spill and am wondering if I need to have the clinic purchase a PAPR to clean up the potential spill. I think that I have to do some sort of fit testing however if I use a PAPR. Can anyone suggest their procedure on how to handle xylene spills in an economical manner? Again we use very little xylene in the lab but our supply comes in one gallon plastic containers. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From ihcworkshop <@t> gmail.com Thu Mar 26 17:21:30 2015 From: ihcworkshop <@t> gmail.com (IHC Workshop) Date: Thu Mar 26 17:21:33 2015 Subject: [Histonet] IHC Workshop for Researchers Message-ID: Hi all: IHC for Researchers; Wet workshop customized for researchers and veterinarians looking to improve their skills in IHC staining: CEU approved, Workshop date: June 25th & 26th, 2015 in San Francisco Bay area For more info reply to this email. From jfray80 <@t> hotmail.com Thu Mar 26 20:15:14 2015 From: jfray80 <@t> hotmail.com (JOSEPH FRAZEE) Date: Thu Mar 26 20:15:19 2015 Subject: [Histonet] Position Wanted Message-ID: The company I am working for is closing their doors in May. I am looking for a Histology Coordinator position . I have over 20 years experience as a Histology Coordinator, and Lab Manager . I also have 2 years as a lab Supervisor with animal tissue . I also have experience in grossing human, and animal tissue. I am qualified under CLIA for grossing . Please reply to this email and I will send you my resume on request . Thank You, Joseph F Frazee HT(ASCP) . From jkiernan <@t> uwo.ca Thu Mar 26 22:46:33 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Mar 26 22:46:38 2015 Subject: Fwd: [Histonet] Phosphotungstic acid In-Reply-To: <7370caa91d6cd.5514d288@uwo.ca> References: <094295B4-606F-432C-BACF-F3AA510DA20A@gmail.com> <736092691f8fb.5514d1c7@uwo.ca> <7360c7411ddb4.5514d204@uwo.ca> <7300d42219871.5514d242@uwo.ca> <7300d0a71e271.5514d245@uwo.ca> <7300f6641e7e6.5514d24a@uwo.ca> <7370caa91d6cd.5514d288@uwo.ca> Message-ID: <73708a2f19678.55148c49@uwo.ca> Dear Cecilia, I hadn't seen the 2009 paper you mention. Please let me know the reference. I've just sent you another email with an attached LM photo, which might be of interest. John Kiernan = = = On 25/03/15, "Cecilia A." wrote: > > > > Hi John, > > > > Thank you for your reply. > > > > In a Developmental Dynamics paper in 2009, PTA was used as a "simple staining method" that produced overall contrast for X-ray imaging soft tissues similar to how Osmium Tetroxide was also used as a soak for this purpose. > > > > We were interested in using PTA as it doesn't render the soft tissue black like Osmium Tetroxide does, so that there's still a chance we would be able to do further histology work on the tissue. However, we wonder what PTA might do to the fixed yet unprocessed tissue when it is soaked for this imaging purpose, and if it might affect its viability for staining and IHC work. > > > > Thank you, > > C. > > > >> On Wed, Mar 25, 2015 at 9:11 AM, John Kiernan wrote: > >> PTA can be used on tiny specimens to enhance electron-opacity. For light microscopy, PTA is used in the trichrome methods to enhance differential staining of collagen and cytoplasm, especially in paraffin sections. These uses of PTA are not the same as "soak formalin fixed soft tissue for a few hours before it gets processed". What are you hoping to achieve? > >> > >> John Kiernan > >> London, Canada > >> = = = > >> > >>> On 25/03/15, "Cecilia A." wrote: > >>> Hello Histonetters, > >>> > >>> I was wondering if anyone might be able to give some input regarding the > >>> use of Phosphotungstic acid in which we want to soak formalin fixed soft > >>> tissue for a few hours before it gets processed. Will subjecting it to PTA > >>> before processing affect its viability for further histology work (ie, H&E > >>> staining and IHC)? > >>> > >>> Thank you very much! > >>> C. > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet@lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jkiernan <@t> uwo.ca Thu Mar 26 22:53:15 2015 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Mar 26 22:53:21 2015 Subject: [Histonet] Tol Blue In-Reply-To: <73009a8719410.5514d403@uwo.ca> References: <1427231919955.28190@animalreferencepathology.com> <7250b8e630e5c.55123cab@uwo.ca> <7330faa63728c.55123ce7@uwo.ca> <7270d6c536d3e.55123d25@uwo.ca> <72b083a6357f6.55123d63@uwo.ca> <72b0a18637a29.55123da1@uwo.ca> <7250a19d33495.55123ddf@uwo.ca> <7270b86736d8e.55123e1e@uwo.ca> <7340d76436b20.55123e5c@uwo.ca> <72e0b3d93669a.55123e9b@uwo.ca> <72d0c94c304ad.55123ed9@uwo.ca> <7250cb2f364d7.55123f18@uwo.ca> <7250d78c31e7f.55123f56@uwo.ca> <72508a6f32584.55123f95@uwo.ca> <71d0a7d130ffc.5512400f@uwo.ca> <733093ff3680a.5512404e@uwo.ca> <72d098d933a55.5512408c@uwo.ca> <7270c11e331f9.551240ce@uwo.ca> <72e0968319618.5514d348@uwo.ca> <73e0a46c1ef56.5514d386@uwo.ca> <7280fe2c1d1f2.5514d3c4@uwo.ca> <73009a8719410.5514d403@uwo.ca> Message-ID: <7300f6c519254.55148ddb@uwo.ca> Mast cells are stained red-purple by toluidine blue. They are defined as cells with granules that stain metachromatically with cationic dyes. You should seek to enhance, not avoid this effect. Metachromasia means staining in a colour different from that of the dye. Toluidine blue is a blue cationic dye, and it imparts its blue (orthochromatic) colour to anionic materials such as nucleic acids. Some sites in tissues have higher concentrations of anions, and this makes the dye cations pile up in layers. Stacking of dye cations changes their absorption of light, and toluidine blue looks red instead of blue. Mast cells contain heparin, which attracts stacks of small dye cations like those of toluidine blue. John Kiernan London, Canada = = = On 24/03/15, Kimberly Marshall wrote: > ?Hello my fellow Histo Techs. Have a question I just know someone out there can answer for me. > > In canine tissue, we are having problems with the Tol Blue for mast cell. Am experiencing metachromasia, or the mast cells turning purple not blue. I have attempted to decrease time, or add time, but its not helping. My pathologist says he has had this issue before. So question is. Could it be the mast cell in a dog does not stain the same? Is there another stain that may work? Any help will be much appreciated. > > > Thanks in Advance. > > Kimberly Marshall H.T.(ASCP) > > > > > > > Kimberly Marshall H.T.(ASCP) > > Histology/Lab Supervisor > > Toll Free 1-800-426-2099 > > Fax 801-584-5104 > > PO Box 17580 > > Salt Lake City, Utah 84107 > > www.animalreferencepathology.com > > > > Advancing the art and science of veterinary medicine > > > > [cid:image001.jpg@01CF8F87.A0BD4830] > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Ryan.Roy <@t> va.gov Fri Mar 27 07:52:55 2015 From: Ryan.Roy <@t> va.gov (Roy, Ryan) Date: Fri Mar 27 07:53:26 2015 Subject: [Histonet] recycled alcohol versus reagent grade alcohol Message-ID: <15F883394EAB744E99E1C7E1B9873049017B48FCA593@R04BYNMSGB1.r04.med.va.gov> Hello Histonet, How do other labs test and verify the percent grade of alcohols and what is the assumed percent grade of reagent alcohol? We use recycled alcohols but today based on not having reagent grade we used 97% tested recycled as our flush. The reasoning behind this was that Reagent grade varies from 95%-greater than 100%. Is this true? Thanks in advance, Ryan From rford <@t> hcmhcares.org Fri Mar 27 08:16:40 2015 From: rford <@t> hcmhcares.org (Rhonda Ford) Date: Fri Mar 27 08:16:44 2015 Subject: [Histonet] Helicobacter pylori controls Message-ID: Does anyone have any extra Helicobacter pylori controls? Thanks. -- Rhonda Ford, Histology Lab Henry County Hospital 1000 North 16th Street New Castle, IN 47362 (765) 521-1148 From imhyper13 <@t> aol.com Fri Mar 27 09:11:37 2015 From: imhyper13 <@t> aol.com (imhyper13@aol.com) Date: Fri Mar 27 09:11:41 2015 Subject: [Histonet] Billing question Message-ID: <14c5b92eb21-ec0-6fe8@webprd-a63.mail.aol.com> I have a question about billing practices for special stains and IHC. We have a single billing code for all of our group 1 special stains. (88312). For example, if you order an AFB, GMS and PASF, it would bill as 88312 x 3. How does everyone else bill in this scenario? We are being told that EACH stain in the group 1 needs it's own billing code. Does anyone else do this? Thanks From DSiena <@t> statlab.com Fri Mar 27 09:38:33 2015 From: DSiena <@t> statlab.com (Debra Siena) Date: Fri Mar 27 09:38:44 2015 Subject: [Histonet] RE: recycled alcohol versus reagent grade alcohol In-Reply-To: <15F883394EAB744E99E1C7E1B9873049017B48FCA593@R04BYNMSGB1.r04.med.va.gov> References: <15F883394EAB744E99E1C7E1B9873049017B48FCA593@R04BYNMSGB1.r04.med.va.gov> Message-ID: Hi Ryan, Reagent grade alcohol can be purchased in different percentages such as 50% to 100%, etc. It depends on what you purchase. Reagent alcohol is simply a blend of three different alcohols, ethanol, methanol, and isopropyl and is created using a formula that is stated by the federal government to make it poisonous for human consumption. Recycled alcohol never comes back as 100%, and does vary from 95% to about 98%. ? Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t:?800.442.3573 ext. 229 | f: 972.767.3992 dsiena@statlab.com | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roy, Ryan Sent: Friday, March 27, 2015 7:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recycled alcohol versus reagent grade alcohol Hello Histonet, How do other labs test and verify the percent grade of alcohols and what is the assumed percent grade of reagent alcohol? We use recycled alcohols but today based on not having reagent grade we used 97% tested recycled as our flush. The reasoning behind this was that Reagent grade varies from 95%-greater than 100%. Is this true? Thanks in advance, Ryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mucram11 <@t> comcast.net Fri Mar 27 09:53:06 2015 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Fri Mar 27 09:53:31 2015 Subject: [Histonet] RE: recycled alcohol versus reagent grade alcohol In-Reply-To: References: <15F883394EAB744E99E1C7E1B9873049017B48FCA593@R04BYNMSGB1.r04.med.va.gov> Message-ID: <1322552838.4478645.1427467986145.JavaMail.zimbra@comcast.net> I would add to that once you open any alcohol and pour it or recap it poorly it will begin to absorb water from the rrom and the percentage is not 100%.? We use a hygrometer and even freshly opened alcohols lose to 1 to 4% on purity very quickly.? We live in a humid climate and some are much worse.? We are also reducing the purity of 100% everytime we add tissue to remove water.? I long ago, stopped worrying about absolute alcohol and started worrying about keeping it above 95% when possible.? ? Pam Marcm ----- Original Message ----- From: "Debra Siena" To: "Ryan Roy" , "Histonet" Sent: Friday, March 27, 2015 9:38:33 AM Subject: [Histonet] RE: recycled alcohol versus reagent grade alcohol Hi Ryan, Reagent grade alcohol can be purchased in different percentages such as 50% to 100%, etc. ?It depends on what you purchase. ? ?Reagent alcohol is simply a blend of three different alcohols, ethanol, methanol, and isopropyl and is created using a formula that is stated by the federal government to make it poisonous for human consumption. ? Recycled alcohol never comes back as 100%, and does vary from 95% to about 98%. ? ? Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t:?800.442.3573 ext. 229 | f: 972.767.3992 dsiena@statlab.com | www.statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roy, Ryan Sent: Friday, March 27, 2015 7:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recycled alcohol versus reagent grade alcohol Hello Histonet, How do other labs test and verify the percent grade of alcohols and what is the assumed percent grade of reagent alcohol? We use recycled alcohols but today based on not having reagent grade we used 97% tested recycled as our flush. The reasoning behind this was that Reagent grade varies from 95%-greater than 100%. Is this true? Thanks in advance, Ryan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From csegovia <@t> nsalabs.com Fri Mar 27 10:10:24 2015 From: csegovia <@t> nsalabs.com (Segovia, Claudia) Date: Fri Mar 27 10:12:01 2015 Subject: [Histonet] IHC workshop Message-ID: Hi, I am interested. Would you please send me more information? Also, do they have a workshop syllabus? Thank you, Claudia N. Segovia Senior Neurohistologist Antibody Specialist NeuroScience Associates 10915 Lake Ridge Drive Knoxville, TN 37934 865-675-2245 csegovia@nsalabs.com STATEMENT OF CONFIDENTIALITY: The information contained in this electronic message and any attachments are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not the intended recipient, or the person responsible for delivering email to the intended recipient, be advised you have received this message in error and any use, dissemination, forwarding, printing or copying is strictly prohibited. Please notify NeuroScience Associates immediately at 865-675-2245 or at csegovia@nsalabs.com, and destroy all copies of this message and any attachments. You will be reimbursed for reasonable costs incurred in notifying us. From BZIMMERM <@t> gru.edu Fri Mar 27 11:05:18 2015 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Fri Mar 27 11:05:27 2015 Subject: [Histonet] HISTOPALOOZA GEORGIA SOCIETY FOR HISTOTECHNOLOGY APRIL 17th - APRIL 19th Woohoo just around the corner Message-ID: The Legacy Lodge at Lake Lanier Islands has released the rooms reserved for Histopalooza, but the GSH room rate will be honored if rooms are AVAILABLE. Please call and ask for Lori at the Lodge if you're still interested in attending. So, join us and say Good bye to winter! This place has gotten great reviews from TripAdvisor. ( Of course, I'm sure there's a Debbie Downer out there somewhere with something negative to say about it.) P.S. I always trust TripAdvisor, too. If they say a place is a dump, then it is!! Looking forward to a great weekend of networking, relaxing, and getting the CEUs in a painless environment. Billie Zimmerman GSH Secretary Born Southern by the Grace of God From egray <@t> hsc.wvu.edu Fri Mar 27 13:33:43 2015 From: egray <@t> hsc.wvu.edu (Gray, Ed) Date: Fri Mar 27 13:33:50 2015 Subject: [Histonet] RE: Billing question (imhyper13@aol.com) Message-ID: They may be referring to a separate CDM (Charge Description Master) each of which would then have the same CPT code attached. This would make it possible to put the stain name on a bill rather than the more generic CPT description. We don't go to the length of creating a CDM for each stain. However, we do create separate CDM's based on price. For all stains done in house we have one code and charge the same price for each. For stains sent to various labs we will create codes which have the appropriate price attached. To take that a step further, those codes are tied to a different cost centers so that cost analysis, pricing decisions and budgeting can be broken down between Histology and Send Outs. Hope this helps, Ed Ed Gray | Clinical Application Coordinator | Phone: 304-293-2945 | Fax: 304-293-1627 | WVU Healthcare l egray@wvuhealthcare.com Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 14 Date: Fri, 27 Mar 2015 10:11:37 -0400 From: imhyper13@aol.com Subject: [Histonet] Billing question To: histonet@lists.utsouthwestern.edu. Message-ID: <14c5b92eb21-ec0-6fe8@webprd-a63.mail.aol.com> Content-Type: text/plain; charset=utf-8 I have a question about billing practices for special stains and IHC. We have a single billing code for all of our group 1 special stains. (88312). For example, if you order an AFB, GMS and PASF, it would bill as 88312 x 3. How does everyone else bill in this scenario? We are being told that EACH stain in the group 1 needs it's own billing code. Does anyone else do this? Thanks ------------------------------ From Kimberly <@t> animalreferencepathology.com Fri Mar 27 16:08:33 2015 From: Kimberly <@t> animalreferencepathology.com (Kimberly Marshall) Date: Fri Mar 27 16:08:41 2015 Subject: [Histonet] Staining dishes and slide racks for sale Message-ID: ?If you still have these very interested in knowing what you are asking. Thanks in advance Kimberly Marshall H.T.(ASCP) Histology/Lab Supervisor Toll Free 1-800-426-2099 Fax 801-584-5104 PO Box 17580 Salt Lake City, Utah 84107 www.animalreferencepathology.com Advancing the art and science of veterinary medicine [cid:image001.jpg@01CF8F87.A0BD4830] From sccrshlly <@t> yahoo.com Fri Mar 27 22:26:52 2015 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Fri Mar 27 22:26:57 2015 Subject: [Histonet] Looking for Newsletter Articles!!! Message-ID: <1555207356.107754.1427513212292.JavaMail.yahoo@mail.yahoo.com> Hello Histonetters!? The Texas Society for Histotechnology is currently accepting articles for our Spring/Summer Histo*TEXas Newsletter!? We require sited sources for all articles and welcome students and professional histology personnel alike.? If you would like to submit an article for our newsletter, please email the article as a Word document (preferrable) or .pdf to mcokertx@gmail.com.? I will be reviewing articles through April 28th. ? Feel free to email me with any questions you might have! Happy Writing,Michelle CokerHisto*TEXas Newsletter Editormcokertx@gmail.com Also...on a side note, if you are a vendor and would be interested in advertising in our wonderful newsletter, feel free to email me for more information. From CObregon <@t> mhs.net Mon Mar 30 10:36:57 2015 From: CObregon <@t> mhs.net (Obregon, Cecilia) Date: Mon Mar 30 10:37:05 2015 Subject: [Histonet] Post fixing IHC slides in formalin In-Reply-To: References: <14c3d541eb9-2d17-14f84@webprd-m104.mail.aol.com>, Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F4F85DBBE@MHSEXMB03.mhs.net> Our facility implemented the post fixing of IHC slides years ago only for tissues like breast, cell blocks, or anything that tends to fall off. We baked the slides in the oven for 30 minutes, and then post fixed them for an additional 30 min in formalin 'fumes'. It works great on breast resections specimens, and it doesn't interfere with staining. Thank you, Cecilia M. Obregon Memorial Regional Hospital 3501 Johsnon Street Hollywood, FL 33021 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Barry Rittman [barryrittman@gmail.com] Sent: Saturday, March 21, 2015 2:20 PM Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Post fixing IHC slides in formalin There are always discussions about fixation but I have never seen comments about using vapor fixation for post fixing or for fixing fresh frozen specimens. Vapor fixation is simple to use and does not require any solvent as it uses the tissue fluids in the case of fresh frozen sections or the solution remaining after IHC. Especially useful for formaldehyde, alcohol, acetone, osmium tetroxide etc. Barry On Sat, Mar 21, 2015 at 12:14 PM, Ann Specian wrote: > Does anyone post fix their IHC slides in formalin in an effort to try to > reduce tissue loss? If so, does anyone have a protocol for this that they > have used and have seen good results? > > > If you have any other suggestions which can help to reduce tissue loss > during IHC staining, I would love to hear from you. > Thanks, > Ann > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. From Kimberly <@t> animalreferencepathology.com Mon Mar 30 11:49:58 2015 From: Kimberly <@t> animalreferencepathology.com (Kimberly Marshall) Date: Mon Mar 30 11:50:09 2015 Subject: [Histonet] Animal reference material Message-ID: <1427734201161.3625@animalreferencepathology.com> ?Happy Monday all... Writing to ask if any of my fellow histo folks can suggest any material/books or any information on staining animal tissue. I am bringing many special stains into the lab and being still new to animal pathology I would like to see if there is any information out there so I can educate myself. Thanks in advcance Kimberly Kimberly Marshall H.T.(ASCP) Histology/Lab Supervisor Toll Free 1-800-426-2099 Fax 801-584-5104 PO Box 17580 Salt Lake City, Utah 84107 www.animalreferencepathology.com Advancing the art and science of veterinary medicine [cid:image001.jpg@01CF8F87.A0BD4830] From abtdhu <@t> gmail.com Mon Mar 30 19:30:40 2015 From: abtdhu <@t> gmail.com (abtdhu@gmail.com) Date: Mon Mar 30 19:30:43 2015 Subject: [Histonet] Re: Post fixing IHC slides In-Reply-To: <55198134.28c73c0a.0484.ffffdb94SMTPIN_ADDED_MISSING@mx.google.com> References: <55198134.28c73c0a.0484.ffffdb94SMTPIN_ADDED_MISSING@mx.google.com> Message-ID: Hi Cecilia, Glad to know post fixation will reduce section falling off from slide. We are bone research lab and always have problem of paraffin bone section falling off after antigen retrieval. There are many ways we have tried, but not improve too much. I know bone section will be easy to fall if it was incompletely fixed. I want to try your post fixation to secure the bone section on the slide. Would you tell me the exact way you are doing? You bake the slides in oven and deparaffin first? Then post fix the slides 30 minutes? How do you do vapor fixation? Then go on for IHC after rinse when you done post fixation? Your answer is very much appreciated. Dorothy > 1. RE: Post fixing IHC slides in formalin (Obregon, Cecilia) > > From: "Obregon, Cecilia" > Subject: RE: [Histonet] Post fixing IHC slides in formalin > > > Our facility implemented the post fixing of IHC slides years ago only for tissues like breast, cell blocks, or anything that tends to fall off. We baked the slides in the oven for 30 minutes, and then post fixed them for an additional 30 min in formalin 'fumes'. It works great on breast resections specimens, and it doesn't interfere with staining. > Thank you, > > Cecilia M. Obregon > Memorial Regional Hospital > 3501 Johsnon Street > Hollywood, FL 33021 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Barry Rittman [barryrittman@gmail.com] > Sent: Saturday, March 21, 2015 2:20 PM > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Post fixing IHC slides in formalin > > There are always discussions about fixation but I have never seen comments > about using vapor fixation for post fixing or for fixing fresh frozen > specimens. > Vapor fixation is simple to use and does not require any solvent as it uses > the tissue fluids in the case of fresh frozen sections or the solution > remaining after IHC. > Especially useful for formaldehyde, alcohol, acetone, osmium tetroxide etc. > Barry > >> On Sat, Mar 21, 2015 at 12:14 PM, Ann Specian wrote: >> >> Does anyone post fix their IHC slides in formalin in an effort to try to >> reduce tissue loss? If so, does anyone have a protocol for this that they >> have used and have seen good results? >> >> >> If you have any other suggestions which can help to reduce tissue loss >> during IHC staining, I would love to hear from you. >> Thanks, >> Ann >> _______________________________________________ >> From hfedor <@t> jhmi.edu Tue Mar 31 06:37:32 2015 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Tue Mar 31 06:37:40 2015 Subject: [Histonet] Vacuum processing of biopsies. Message-ID: Hello all, I would be interested in pros and cons of using one of the Rapid vacuum processing units for processing of biopsy tissues. Is this technology improving the processing? Any specific problems that may be occurring. Thanks in advance. Helen L. Fedor Oncology Tissue Services, Manager Johns Hopkins University 411 N. Caroline St Room 310 Basement| Bond St Annex Building Baltimore, MD | 21231 410-614-1660 http://tmalab.jhmi.edu/ From foreightl <@t> gmail.com Tue Mar 31 08:17:43 2015 From: foreightl <@t> gmail.com (Patrick Laurie) Date: Tue Mar 31 08:17:48 2015 Subject: [Histonet] Animal reference material In-Reply-To: <1427734201161.3625@animalreferencepathology.com> References: <1427734201161.3625@animalreferencepathology.com> Message-ID: The best that I have used in the past was humason's animal tissue techniques. I think that there are several editions out there, I used a 1979 edition. After googling it, I see that there may be a full text version available online too. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Mon, Mar 30, 2015 at 12:49 PM, Kimberly Marshall < Kimberly@animalreferencepathology.com> wrote: > ?Happy Monday all... > > Writing to ask if any of my fellow histo folks can suggest any > material/books or any information on staining animal tissue. I am bringing > many special stains into the lab and being still new to animal pathology I > would like to see if there is any information out there so I can educate > myself. > > Thanks in advcance > > Kimberly > > > > > Kimberly Marshall H.T.(ASCP) > > Histology/Lab Supervisor > > Toll Free 1-800-426-2099 > > Fax 801-584-5104 > > PO Box 17580 > > Salt Lake City, Utah 84107 > > www.animalreferencepathology.com > > > > Advancing the art and science of veterinary medicine > > > > [cid:image001.jpg@01CF8F87.A0BD4830] > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Terra.Wineman <@t> novusint.com Tue Mar 31 08:52:44 2015 From: Terra.Wineman <@t> novusint.com (Wineman, Terra) Date: Tue Mar 31 08:52:57 2015 Subject: [Histonet] Animal reference material In-Reply-To: References: <1427734201161.3625@animalreferencepathology.com> Message-ID: <1EB8F245A303564EADF12AC7022FA74DD76D521F@novus-ex01> Here is a link I found on google. https://archive.org/details/animaltissuetech00huma Terra Wineman, HTL (ASCP)CM Research Biologist, Physiology 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Tuesday, March 31, 2015 8:18 AM To: Kimberly Marshall Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Animal reference material The best that I have used in the past was humason's animal tissue techniques. I think that there are several editions out there, I used a 1979 edition. After googling it, I see that there may be a full text version available online too. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Mon, Mar 30, 2015 at 12:49 PM, Kimberly Marshall < Kimberly@animalreferencepathology.com> wrote: > ?Happy Monday all... > > Writing to ask if any of my fellow histo folks can suggest any > material/books or any information on staining animal tissue. I am > bringing many special stains into the lab and being still new to > animal pathology I would like to see if there is any information out > there so I can educate myself. > > Thanks in advcance > > Kimberly > > > > > Kimberly Marshall H.T.(ASCP) > > Histology/Lab Supervisor > > Toll Free 1-800-426-2099 > > Fax 801-584-5104 > > PO Box 17580 > > Salt Lake City, Utah 84107 > > www.animalreferencepathology.com om/> > > > > Advancing the art and science of veterinary medicine > > > > [cid:image001.jpg@01CF8F87.A0BD4830] > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Tue Mar 31 10:03:07 2015 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Mar 31 10:03:16 2015 Subject: [Histonet] Animal reference material In-Reply-To: <1EB8F245A303564EADF12AC7022FA74DD76D521F@novus-ex01> References: <1427734201161.3625@animalreferencepathology.com> , <1EB8F245A303564EADF12AC7022FA74DD76D521F@novus-ex01> Message-ID: After working for over twenty years on human tissues in clinical labs I went to work in a research core lab. I was the only histotech so I had to learn about animal, insect and plant tissue plus some things like cell pellets and films. I thank my connections from networking thru NSH and state societies who always came through with the perfect advice so don't NOT use this resource. I found that there wasn't much difference with routine special stains like trichrome, PAS, LFB, oil red o, etc. You may be asked to do some that aren't usually done on human tissue. I did a lot of picrosirius red and a special elastic stain for lung tissue. I was asked to combine some stains like H&E-alcian blue. It really depends on what the people do who are bringing you the tissue. Since my lab served researchers I could expect almost anything! Freida Carson's book is a good book to have on hand. I liked the second edition most. If you can get your hands on an old AFIP manual and a Preece get them! You may find differences with IHC. Can't help you there since that was done in another lab. Processing is where the differences lie. Make sure you get some good info on processing other species. Humason is good and don't forget the NSH has an Animal Processing Manual. I found a lot of protocols online. I had different schedules for different species and types of tissue - like mouse embryo or chick embryo, brains, bones, tiny little things like cultured mouse ovaries and on and on. Good luck! Andi G. Happily retired but soon to return to Histo (just a little) ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Wineman, Terra [Terra.Wineman@novusint.com] Sent: Tuesday, March 31, 2015 6:52 AM To: Patrick Laurie; Kimberly Marshall Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Animal reference material Here is a link I found on google. https://archive.org/details/animaltissuetech00huma Terra Wineman, HTL (ASCP)CM Research Biologist, Physiology 636-926-7476 phone terra.wineman@novusint.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Tuesday, March 31, 2015 8:18 AM To: Kimberly Marshall Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Animal reference material The best that I have used in the past was humason's animal tissue techniques. I think that there are several editions out there, I used a 1979 edition. After googling it, I see that there may be a full text version available online too. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Mon, Mar 30, 2015 at 12:49 PM, Kimberly Marshall < Kimberly@animalreferencepathology.com> wrote: > ?Happy Monday all... > > Writing to ask if any of my fellow histo folks can suggest any > material/books or any information on staining animal tissue. I am > bringing many special stains into the lab and being still new to > animal pathology I would like to see if there is any information out > there so I can educate myself. > > Thanks in advcance > > Kimberly > > > > > Kimberly Marshall H.T.(ASCP) > > Histology/Lab Supervisor > > Toll Free 1-800-426-2099 > > Fax 801-584-5104 > > PO Box 17580 > > Salt Lake City, Utah 84107 > > www.animalreferencepathology.com om/> > > > > Advancing the art and science of veterinary medicine > > > > [cid:image001.jpg@01CF8F87.A0BD4830] > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CObregon <@t> mhs.net Tue Mar 31 10:42:06 2015 From: CObregon <@t> mhs.net (Obregon, Cecilia) Date: Tue Mar 31 10:42:14 2015 Subject: [Histonet] Re: Post fixing IHC slides In-Reply-To: References: <55198134.28c73c0a.0484.ffffdb94SMTPIN_ADDED_MISSING@mx.google.com>, Message-ID: <598BB4A3A92D7F4DAA14C30E4AE70C2F4F85EF33@MHSEXMB03.mhs.net> We bake our slide in the oven for 30 minutes at 65 degrees. Then placed them in a staining bucket 'covered' at room temperature for 30 minutes. This small bucket contains a piece of gauze soaked in formalin at the bottom. We do not 'immerse' the slides in formalin, we just sort of use the contained fumes to post fix the slides. No rinsing after this step, just load them in the Benchmark Ultra, and coverslip as usual. (We do manually rinse all of our slides in Di soapy water before coverslipping..whether they have been post fixed or not to remove excess LCS) Hope this helps, I can be reached at 954-265-5317 if you have any further questions. Thanks-Cecilia ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of abtdhu@gmail.com [abtdhu@gmail.com] Sent: Monday, March 30, 2015 8:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Post fixing IHC slides Hi Cecilia, Glad to know post fixation will reduce section falling off from slide. We are bone research lab and always have problem of paraffin bone section falling off after antigen retrieval. There are many ways we have tried, but not improve too much. I know bone section will be easy to fall if it was incompletely fixed. I want to try your post fixation to secure the bone section on the slide. Would you tell me the exact way you are doing? You bake the slides in oven and deparaffin first? Then post fix the slides 30 minutes? How do you do vapor fixation? Then go on for IHC after rinse when you done post fixation? Your answer is very much appreciated. Dorothy > 1. RE: Post fixing IHC slides in formalin (Obregon, Cecilia) > > From: "Obregon, Cecilia" > Subject: RE: [Histonet] Post fixing IHC slides in formalin > > > Our facility implemented the post fixing of IHC slides years ago only for tissues like breast, cell blocks, or anything that tends to fall off. We baked the slides in the oven for 30 minutes, and then post fixed them for an additional 30 min in formalin 'fumes'. It works great on breast resections specimens, and it doesn't interfere with staining. > Thank you, > > Cecilia M. Obregon > Memorial Regional Hospital > 3501 Johsnon Street > Hollywood, FL 33021 > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Barry Rittman [barryrittman@gmail.com] > Sent: Saturday, March 21, 2015 2:20 PM > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Post fixing IHC slides in formalin > > There are always discussions about fixation but I have never seen comments > about using vapor fixation for post fixing or for fixing fresh frozen > specimens. > Vapor fixation is simple to use and does not require any solvent as it uses > the tissue fluids in the case of fresh frozen sections or the solution > remaining after IHC. > Especially useful for formaldehyde, alcohol, acetone, osmium tetroxide etc. > Barry > >> On Sat, Mar 21, 2015 at 12:14 PM, Ann Specian wrote: >> >> Does anyone post fix their IHC slides in formalin in an effort to try to >> reduce tissue loss? If so, does anyone have a protocol for this that they >> have used and have seen good results? >> >> >> If you have any other suggestions which can help to reduce tissue loss >> during IHC staining, I would love to hear from you. >> Thanks, >> Ann >> _______________________________________________ >> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. From cynthia.hunter <@t> crystaldatalist.com Mon Mar 30 10:10:38 2015 From: cynthia.hunter <@t> crystaldatalist.com (Cynthia Hunter) Date: Tue Apr 14 15:40:29 2015 Subject: [Histonet] OB/GYN Database Message-ID: Hi, I want to check if you're interested in OB/GYN Database? Specialties- Pregnancy Care, Family Practice, Internal Med, Family Med, Psychiatry, Independent Practices, Hospital Owned and many more.. The lists comes with complete details such as Name, Company, Website, Title, Phone number, Fax number, Email address, Country, SIC code, zip code, revenue & employees. Our Capabilities: * Email campaigns with dedicated Login credential to check the reports on real time Basis *Appending Services: We can append any missing information on your existing database and append additional information too making sure all the white Space is filled *Digital Marketing: We execute Social Media campaigns. And assist on SEO, Website Design & Development and Content Marketing Let me know a convenient time to schedule a call and help us give you more insights. Look forward speaking with you and assuring our list will fetch better results. Best regards Cynthia Hunter Online Marketing Executive If you're not interested to receive further emails, please reply with the subject line as "Leave out" From emily.jacob <@t> crystaldatalist.com Mon Mar 30 11:06:31 2015 From: emily.jacob <@t> crystaldatalist.com (Emily Jacob) Date: Tue Apr 14 15:40:31 2015 Subject: [Histonet] Healthcare Specialist Message-ID: Hi, I hope you are the right person to discuss about Healthcare Specialist Database - 2013 which comprise complete contact details (verified Phone Number, Fax Number, Verified Email Address, Employee Size, Revenue size, SIC Code, Industry Type and many more). Few of the lists mentioned: Anesthesiology Doctors , Cardiology, Chiropractic Doctors, Dermatology Doctors, Emergency Medicine Doctors, Family Practice, Gastroenterology, Gynecology Doctors , Hematology , Internal medicine Doctors, Neurology Doctors, Obstetrics/Gynecology Doctors , Oncology Doctors, Ophthalmology Doctors, Optometry Doctors, Orthopedic Surgery, Otorhinolaryngology Doctors, Pathology Doctors, Pediatrics Doctors, Plastic Surgery, Psychiatry, Radiology, Urology Doctors, Nurse Mailing, Registered Nurses and many more. Please let me know your target audience and geographical area, so that we can send you more information about our services. Looking forward to hear from you. Regards, Emily Jacob | List acquisition | Technology Lists | Email/Data Appending | Search Engine Optimization | If you do not wish to receive future emails from us, please reply as 'leave out' From 11z <@t> comcast.net Tue Mar 24 16:48:00 2015 From: 11z <@t> comcast.net (LeRoy Brown) Date: Thu Apr 23 11:51:20 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <18D791D4EE07BC41BF05F8EF3CCCDE24678E4765@FTCSEAP4001.nam.int.local> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> <643626B74DE2814D8537057F40E1A10B15506756@CSI-MX-NODEA.CSI-LABS.local> <18D791D4EE07BC41BF05F8EF3CCCDE24678E4765@FTCSEAP4001.nam.int.local> Message-ID: <002a01d0667c$2f30ce90$8d926bb0$@comcast.net> Dear Nancy Stedman, Thank you for your confidence in our work. I have been doing histology for over 45 years and have seen so many changes for the better. Histology is a high calling to those who take it seriously. I have indeed seen some pathologist that have not appreciated the people who do the work that enables them to have a job. Thankfully they are few and far between. There is not a day that goes by that I am not thankful for the field I choose way back in 1970. Roy Brown -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stedman, Nancy Sent: Tuesday, March 24, 2015 2:22 PM To: Mark Turner; Paula Sicurello; Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Timothy Morken; Jennifer MacDonald; Marcum, Pamela A Subject: RE: [Histonet] BS in Histotechnology As a pathologist I'd like to apologize for all the pathologists who have made comments like this.. forget trained monkeys and dogs, most (all?) pathologists cannot cut slides either, at least not slides they'd want to try to read. I know I can't. -Nancy Stedman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Tuesday, March 24, 2015 4:26 PM To: Paula Sicurello; Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Timothy Morken; Jennifer MacDonald; Marcum, Pamela A Subject: RE: [Histonet] BS in Histotechnology I once worked with a Pathologist who said she was in a group meeting of other pathologists when one of them blurted out that a trained monkey could cut slides. My pathologist, having had the opportunity to review some cases from the offender's laboratory, promptly replied "Yes, and with the quality of your slides it looks like you did just that." She shut down the other pathologist really quickly, and as far as I know, we never received another case to review from him. My pathologist was not about to let that kind of arrogance stand. She was one of the best bosses I ever had! Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, March 24, 2015 3:47 PM To: Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, Pamela A; Timothy Morken Subject: Re: [Histonet] BS in Histotechnology I've had more than one pathologist tell me a monkey could do my job. Though one of them said it with a smile and added "a very highly skilled and well trained monkey", he was one of the few who knew better. How many of us monkeys have trained the whining and complaining residents how to do things correctly? Paula On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones wrote: > OMG Pam~ our pathologist said the exact same thing to us when we > started our Grossing training. > Sheesh. . . > Michael Ann > > > > > On 3/24/15, 11:52 AM, "Marcum, Pamela A" wrote: > > >That was nicer than the pathologist who told me years ago, "any > >monkey could be trained to do my job". I basically did not take the > >job I was interviewing for at the time. At least the next interview > >went a lot better and I did take the job. > > > >Pam > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > >Sanders, Jeanine (CDC/OID/NCEZID) > >Sent: Tuesday, March 24, 2015 12:30 PM > >To: Sue; Timothy Morken > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >Subject: RE: [Histonet] BS in Histotechnology > > > >I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can > >teach any trained dog how to section histology will never have the > >recognition those of us that have studied and trained deserve. > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue > >Sent: Tuesday, March 24, 2015 12:59 PM > >To: Timothy Morken > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >Subject: Re: [Histonet] BS in Histotechnology > > > >This is a fight that we continue to have with hospital administration. > >In my opinion histologists are just as important and needed as MT. > >Even though there is an increase in automation in pathology the hands > >on of a histologists is most important. The fact that hospital still > >consider a lower entry job is the reason there are not more of us. > >It is quite frustrating. > > > >Sue > >TJUH > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >--------------------------------------------------------------------- > >- Confidentiality Notice: This e-mail message, including any > >attachments, is for the sole use of the intended recipient(s) and may > >contain confidential and privileged information. Any unauthorized > >review, use, disclosure or distribution is prohibited. If you are not > >the intended recipient, please contact the sender by reply e-mail and > >destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmcampbe <@t> fmh.org Mon Mar 16 10:38:06 2015 From: tmcampbe <@t> fmh.org (Campbell, Tasha M.) Date: Thu Apr 23 12:14:45 2015 Subject: [Histonet] Need temporary Histotech Message-ID: Hello everyone, I was wondering if there is anyone out there that does temporary work in histo labs in the Frederick MD area. I run a small GI lab and I will be going on maternity leave in October and we would like someone to fill in. I know that there are temp agencies I can go through, but I thought I would try leaving the middle man out first because we are a small lab and are trying to save money and they can have more costs. I would need someone for 8 wks. The tech would have to accession, gross the biopsies and of course process, embed, cut and stain. I also do warthin starry and ABPAS by hand. There are on average 30 blocks a day and 10 specials a day. You do your own thing. Go at your own pace. Flexible time. No one bothers you. Just as long as the work gets done. It's really a walk in the park. If there is anyone in the area that is willing to do this, please email or call me! Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) From tmcampbe <@t> fmh.org Mon Mar 16 11:11:49 2015 From: tmcampbe <@t> fmh.org (Campbell, Tasha M.) Date: Thu Apr 23 12:14:50 2015 Subject: [Histonet] List Message-ID: Hello, I posted something to histonet and it said that I was a nonmember and it was awaiting approval but I have been a member for 2 years. Is something wrong with the server? Thanks. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) From sccrshlly <@t> yahoo.com Wed Mar 11 22:07:10 2015 From: sccrshlly <@t> yahoo.com (Shelly Coker) Date: Thu Apr 23 12:14:54 2015 Subject: [Histonet] Re: Masson's Trichrome Message-ID: <1933374883.4377024.1426129624751.JavaMail.yahoo@mail.yahoo.com> PTPM Acid is the bridge between the collagen and the aniline blue.? It links the dye to the collagen.? If you rinse the PTPM acid before putting the slide in the Aniline blue, you will not get the desired intensity of staining (of the collagen) that you are looking for. Cheers and Have a great week! > Here is a message from Justine...> > From: Justine Lanzon [mailto:justinelanzon@hotmail.com] > > Sent: Thursday, March 05, 2015 5:36 AM > To: lindamargraf@gmail.com > Subject: Masson's trichrome stain > > > Hi, > > I am doing a write up on Masson's trichrome stain however I cannot > answer these two questions: > > - Why are plastic forceps used instead of metal ones to hold the > stained slide? > > - Why do we not rinse before Alinine blue? > > ? > > Can you please help me? > > ? > > Many Thanks, > > Justine Lanzon > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ We have literally about one hundred slides to re-slip for the this reason.? Are there any suggestions for large numbers of slides to be re-coverslipped as this method would be too time consuming.? We have used only glass for about nine years or so and it is much better.? The old ones are the problem when someone needs "THAT" slide only. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mayer,Toysha N Sent: Wednesday, March 11, 2015 10:43 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Old slides Bernice, Take the slide and dip it in xylene.? Lay it on the film, pressing down firmly.? As it adheres, then gently wipe the excess xylene off, and gently place it in a book or your procedure manual and leave it there for an hour or so. Most of the bubbles will be gone, and the tissue will be saved. The original problem is not enough xylene dispersed onto the slide.? Adjust the flow being dispensed by the unit.? Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Mar 2015 19:41:48 +0000 From: Bernice Frederick Subject: [Histonet] Old slides. To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? Content-Type: text/plain; charset="us-ascii" Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Hello, We received some liver tissue (Mouse) in 10% formalin (NFB). Then transferred to 70% ETOH. My question is? that, Is it ok to transfer? to 30% sucrose? So, frozen section can be perform. Please advise. Thanks, Bryan S. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, March 10, 2015 10:06 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 136, Issue 12 Send Histonet mailing list submissions to ??? histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. Old slides. (Bernice Frederick) ? 2. RE: Old slides. (Jason McGough) ? 3. RE: Mushrooms for GMS fungus control (Morken, Timothy) ? 4. Re: FW: Masson's trichrome stain (John Kiernan) ? 5. RE: Old slides. (John Kiernan) ? 6. soft for microwriter (thermo scientific, Lamb, Shandon) ? ? ? (richard wild) ? 7. Re: Old slides. (b.curran.mcwilliam@gmail.com) ? 8. Re: Old slides. (Rene J Buesa) ? 9. RE: Old slides. (Gowan,Christie C) ? 10. IHC / Morphometry Technician wanted in Shenandoah Valley ? ? ? Virginia (Erin Sarricks) ---------------------------------------------------------------------- Message: 1 Date: Mon, 9 Mar 2015 19:41:48 +0000 From: Bernice Frederick Subject: [Histonet] Old slides. To: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? Content-Type: text/plain; charset="us-ascii" Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu ------------------------------ Message: 2 Date: Mon, 9 Mar 2015 14:20:09 -0600 From: Jason McGough Subject: RE: [Histonet] Old slides. To: Bernice Frederick , ??? histonet@lists.utsouthwestern.edu ??? Message-ID: Content-Type: text/plain; charset=utf-8 Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgough@clinlab.com www.clinlab.com -----Original message----- > From:Bernice Frederick > > Sent: Monday, March 9, 2015 1:51 PM > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Old slides. > > Hi all, > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. > Thanks, > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ Message: 3 Date: Mon, 9 Mar 2015 22:46:08 +0000 From: "Morken, Timothy" Subject: [Histonet] RE: Mushrooms for GMS fungus control To: "koellingr@comcast.net" Cc: "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <761E2B5697F795489C8710BCC72141FF367FBA31@ex07.net.ucsf.edu> Content-Type: text/plain; charset="utf-8" Try this article... Acta Cytol. 2003 Nov-Dec;47(6):1043-4. Alternative, cost-effective fungus-staining method for control slides in cytology and histopathology. da Silva VD1. Author information Abstract OBJECTIVE: To develop a cost-effective, reliable and safe method of providing fungal control slides for routine use in pathology laboratories. STUDY DESIGN: A set of easily available, low-cost material was tested to obtain fungal colonies on substrate adequate for paraffin-embedded sections or smears. RESULTS: Such material as cheese is a simple, inexpensive and practical culture medium for silver-positive fungi. A batch of paraffin blocks can be prepared to maintain a stock of control material in the laboratory. CONCLUSION: It is useful to maintain fungal colonies to produce staining control specimens using small pieces of refrigerated cheese to easily produce silver-staining control specimens or smears embedded in paraffin, reducing the risk of accidental exposure to potentially infective pathogens in the laboratory. This method might also be a good alternative for conserving routine surgical specimens, considering the currently decreasing numbers of necropsy and large specimens, particularly from immunosuppressed and infected patients. PMID: 14674076 [PubMed - indexed for MEDLINE] -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of koellingr@comcast.net Sent: Sunday, March 08, 2015 4:29 PM To: Linda Prasad (SCHN) Cc: Jeffrey Robinson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Mushrooms for GMS fungus control Apparently there are numerous interesting ways for fungus or bacteria controls to be had from orange peels to hamburger to slim Jim's to hot dogs to strawberries to ????.?? Sounds like fun to me.?? I'm curious, with the emphasis now on quality control in labs??run amok, has anyone passed a rigorous inspection actually showing these as your currently in-use controls??? A PI in research who??doesn't want??his paper rejected at peer review.?? A CAP inspector in clinical labs who is nit-picky reviewing staining controls but might be looking for a phase anything deficiency.?? The??dot-your-i's and cross-your-t's??FDA people who might or might not OK your drug in development.?? Really, just curious if anyone with a hammer over your head has said it is perfectly fine to use them. Ray, Seattle, WA ----- Original Message ----- From: "Linda Prasad (SCHN)" To: "Jeffrey Robinson" , histonet@lists.utsouthwestern.edu Sent: Sunday, March 8, 2015 4:09:02 PM Subject: [Histonet] RE: Mushrooms for GMS fungus control I used strawberries for a fungal control. Worked really good. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???????Please consider the environment before printing this email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Saturday, 7 March 2015 4:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mushrooms for GMS fungus control How about mushrooms? ??Has anyone had any success using mushrooms as a GMS fungus control? Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 10 Mar 2015 00:31:56 -0500 From: John Kiernan Subject: Re: [Histonet] FW: Masson's trichrome stain To: Linda Margraf , ??? histonet@lists.utsouthwestern.edu Cc: justinelanzon@hotmail.com Message-ID: <7380eaed48941.54fe3b7c@uwo.ca> Content-Type: text/plain; charset=iso-8859-1 The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? John Kiernan = = = On 08/03/15, Linda Margraf? wrote: > Here is a message from Justine... > > From: Justine Lanzon [mailto:justinelanzon@hotmail.com] > > Sent: Thursday, March 05, 2015 5:36 AM > To: lindamargraf@gmail.com > Subject: Masson's trichrome stain > > > Hi, > > I am doing a write up on Masson's trichrome stain however I cannot > answer these two questions: > > - Why are plastic forceps used instead of metal ones to hold the > stained slide? > > - Why do we not rinse before Alinine blue? > > ? > > Can you please help me? > > ? > > Many Thanks, > > Justine Lanzon > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 5 Date: Tue, 10 Mar 2015 00:40:02 -0500 From: John Kiernan Subject: RE: [Histonet] Old slides. To: Jason McGough ,??? Bernice Frederick ??? ,??? histonet@lists.utsouthwestern.edu, ??? histonet@lists.utsouthwestern.edu Message-ID: <73e09aa74b442.54fe3d62@uwo.ca> Content-Type: text/plain; charset=iso-8859-1 Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one. John Kiernan Anatomy & Cell Biology, UWO London, Canada = = = On 09/03/15, Jason McGough? wrote: > Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. > > > > Jason McGough, HT(ASCP) > > Operations Manager > > Clinical Laboratory of the Black Hills > > 605-343-2267 > > jmcgough@clinlab.com > > > www.clinlab.com > > ? > ? > -----Original message----- > > From:Bernice Frederick > > > > > > > Sent: Monday, March 9, 2015 1:51 PM > > To: histonet@lists.utsouthwestern.edu > > > > > > Subject: [Histonet] Old slides. > > > > Hi all, > > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 6 Date: Tue, 10 Mar 2015 10:05:11 +0100 From: richard wild Subject: [Histonet] soft for microwriter (thermo scientific, Lamb, ??? Shandon) To: histonet@lists.utsouthwestern.edu Message-ID: <54FEB3C7.40306@wanadoo.fr> Content-Type: text/plain; charset=utf-8; format=flowed Hi I am looking for labelling software (or advices) for the carousel microwriter (thermo scientific, Lamb, Shandon = same machine) (LAMB E22.01MWR) The machine is discontinuated. I would like to use serial interface rs232 and barcode scanners. Thanks for help. Richard ------------------------------ Message: 7 Date: Tue, 10 Mar 2015 11:51:25 +0000 From: b.curran.mcwilliam@gmail.com Subject: Re: [Histonet] Old slides. To: Bernice Frederick Cc: "histonet@lists.utsouthwestern.edu" ??? Message-ID: <9FA4B5FB-11BE-47C7-AA0C-6F8416B10487@gmail.com> Content-Type: text/plain;??? charset=us-ascii Hi We re-Coverslipper a number of sections which had peeled off on the tape as the tape dried and curled. We cut off excess tape using scissors; placed a fresh coverslip flat; put a streak of mounting medium on he coverslip; use a forceps to orientate the tissue + margin of tape; number a slide, dip it in Xylene & place on a slope & bring on top of coverslip-section-mounting medium; turn the slide-section- coverslip to face coverslip up; leave horizontal to dry (eg overnight). Worth a try, doing one first. Bernie, St Vincent's,? Dublin, Ireland > On 9 Mar 2015, at 19:41, Bernice Frederick wrote: > > Hi all, > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. > Thanks, > Bernice > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick@northwestern.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 10 Mar 2015 13:43:35 +0000 (UTC) From: Rene J Buesa Subject: Re: [Histonet] Old slides. To: John Kiernan , Jason McGough ??? , ??? Bernice Frederick ??? , ??? "histonet@lists.utsouthwestern.edu" ??? Message-ID: ??? <1897656965.1753669.1425995015431.JavaMail.yahoo@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 To John Probably the writer is referring to slides "mounted" with the Sakura film??"coverslip".I have done it many times and the FILM will??detach easily from the section.Had it been a glass coverslip attached with, for example, Permount, acetone would not have done anything.Ren?? J.?? ? ? On Tuesday, March 10, 2015 1:40 AM, John Kiernan wrote: ? Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one. John Kiernan Anatomy & Cell Biology, UWO London, Canada = = = On 09/03/15, Jason McGough?? wrote: > Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. > > > > Jason McGough, HT(ASCP) > > Operations Manager > > Clinical Laboratory of the Black Hills > > 605-343-2267 > > jmcgough@clinlab.com > > > www.clinlab.com > > ?? > ?? > -----Original message----- > > From:Bernice Frederick > > > > Sent: Monday, March 9, 2015 1:51 PM > > To: histonet@lists.utsouthwestern.edu > > > Subject: [Histonet] Old slides. > > > > Hi all, > > We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Senior Research Tech > > Pathology Core Facility > > Robert. H. Lurie Cancer Center > > Northwestern University > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > b-frederick@northwestern.edu > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ? ------------------------------ Message: 9 Date: Mon, 9 Mar 2015 20:01:10 +0000 From: "Gowan,Christie C" Subject: [Histonet] RE: Old slides. To: "'Bernice Frederick'" , ??? "histonet@lists.utsouthwestern.edu" ??? Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Bernice, I have found that if you flood the slide with mounting media (don't use xylene) flip the slide over onto an absorbent lab wipe and put a heavy weight with even pressure and leave for a few hours. If the slide sticks to the wipe just put a few drops of xylene to clean up the slide. You may still have some tiny bubbles but it is much better than the alternative. Good luck. Christie Gowan -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, March 09, 2015 3:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick@northwestern.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 10 Mar 2015 12:12:05 -0400 From: Erin Sarricks Subject: [Histonet] IHC / Morphometry Technician wanted in Shenandoah ??? Valley??? Virginia To: histonet Message-ID: ??? Content-Type: text/plain; charset=UTF-8 Histology Laboratory located in the Shenandoah Valley of Virginia is looking to add to it's team. In this position, you will need a working knowledge of IHC theory and practical IHC experience. The best candidate for the position will oversee immunohistochemical staining as well as perform other histology functions including trimming of specimens, paraffin embedding, microtomy and microscopic QC of slides. Experience with morphometry is preferable. Desirable candidates will possess the following: - HT (ASCP) or QIHC registration preferred - 4 years of Histology experience - 1+ years of immunohistochemistry and/or immunofluorescence experience - Keeps abreast with company's current policies and immunohistochemistry technical updates and procedures - Must be able to work independently and in a team environment Full time employment benefits include subsidized medical and dental insurance, vacation, holiday pay, and 401k after 1 year of employment. Compensation is commensurate with experience. If you are interested in this position, please respond to this post with your resume and cover letter. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 136, Issue 12 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dermacklab <@t> gmail.com Tue Mar 17 12:55:50 2015 From: dermacklab <@t> gmail.com (Jeff McKenna) Date: Thu Apr 23 12:15:02 2015 Subject: [Histonet] Anyone need a Benchmark Ultra? Message-ID: Is anyone looking for an extra Ventana Benchmark Ultra for IHC/ISH? I have one in good shape that is not being used anymore and I am looking to free up some space. Email me if you are interested and I'll give you more info on it. Jeff Mack dermacklab@gmail.com (302) 827-3685 From dgoodwin <@t> rwjuhh.edu Mon Mar 23 13:08:45 2015 From: dgoodwin <@t> rwjuhh.edu (Goodwin, Diana) Date: Thu Apr 23 12:15:06 2015 Subject: [Histonet] FW: NJSH 2015 Summer Meeting In-Reply-To: <667F0DDD9B242E40B448C2CDED9409970A644ABAFB@exch-ms-02.PHCS.ORG> References: <667F0DDD9B242E40B448C2CDED9409970A644ABAFB@exch-ms-02.PHCS.ORG> Message-ID: <09411E0112A96A459D8D5FBDAB9C15C72CEC2B9508@HAMEXMBA.rwjham.local> ________________________________ From: goodwin, diana [dgoodwin@princetonhcs.org] Sent: Monday, February 16, 2015 11:00 AM To: Goodwin, Diana Subject: NJSH 2015 Summer Meeting SAVE THE DATE! The New Jersey Society for Histotechnology Announces the 2015 Summer Meeting Date: Friday, June 19th Sponsored By: Biocare Medical, LLC Speakers: Jason Ramos, PhD and Thomas Ehlers, MS Meeting Location: New Jersey Hospital Association Conference Center in Princeton, NJ Tentative Schedule 8:00-8:30 Registration and Continental Breakfast 8:30-10:00 ?Cell Biology and Immunology for IHC? (1.5 CEU) 10:00-11:00 ?Factors Influencing Antibody Performance, Selection & Validation? (1.0 CEU) 11:00-12:30 ?IHC Troubleshooting? (1.5 CEU) 12:30-1:30 LUNCH 1:30-2:00 ?Power of pH? (0.5 CEU) 2:00-3:30 ?Molecular Pathology Overview? (1.5 CEU) 3:30-4:30 COFFEE BREAK BONUS SESSION: Introduction to the intelliPATHTM Automated Slide Staining System, with a demonstration of both the clinical & research software (NO CEUs will be granted since this presentation is product specific.) Reserve the date now, and look for more details and registration information at the beginning of April! Diana Goodwin Anatomic Pathology Supervisor Office 16808 Histology 16860 ________________________________ This e-mail transmission and any documents attached hereto contain information from Princeton HealthCare System which is confidential and/or legally privileged. This information is intended only for the use of the individual or entity to which it is addressed, and only for the purpose for which this transmission was made. Any other use, disclosure, copying, distribution, re-transmission, or the taking of any action in reliance upon the contents of this information is strictly prohibited. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, re-transmission or the taking of any action in reliance upon the contents of this information is strictly prohibited, and that this e-mail transmission and accompanying documents should be returned to Princeton HealthCare System immediately. If you have received this e-mail transmission in error, please notify the sender immediately and destroy all copies of the information contained in this transmission. The above signature block is intended for identification purposes only and does not constitute affirmation that a binding contract has been created via this e-mail communication unless expressly so stated within the body of this e-mail transmission. ________________________________ From suetp918 <@t> comcast.net Tue Mar 24 16:51:24 2015 From: suetp918 <@t> comcast.net (suetp918) Date: Thu Apr 23 12:15:11 2015 Subject: [Histonet] BS in Histotechnology Message-ID: Thank u dr.stedman Sent from my Verizon Wireless 4G LTE smartphone -------- Original message -------- From: "Stedman, Nancy" Date:03/24/2015 5:22 PM (GMT-05:00) To: Mark Turner , Paula Sicurello , Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu, Timothy Morken , Jennifer MacDonald , "Marcum, Pamela A" Subject: RE: [Histonet] BS in Histotechnology As a pathologist I'd like to apologize for all the pathologists who have made comments like this.. forget trained monkeys and dogs, most (all?) pathologists cannot cut slides either, at least not slides they'd want to try to read. I know I can't. -Nancy Stedman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Tuesday, March 24, 2015 4:26 PM To: Paula Sicurello; Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Timothy Morken; Jennifer MacDonald; Marcum, Pamela A Subject: RE: [Histonet] BS in Histotechnology I once worked with a Pathologist who said she was in a group meeting of other pathologists when one of them blurted out that a trained monkey could cut slides. My pathologist, having had the opportunity to review some cases from the offender's laboratory, promptly replied "Yes, and with the quality of your slides it looks like you did just that." She shut down the other pathologist really quickly, and as far as I know, we never received another case to review from him. My pathologist was not about to let that kind of arrogance stand. She was one of the best bosses I ever had! Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, March 24, 2015 3:47 PM To: Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, Pamela A; Timothy Morken Subject: Re: [Histonet] BS in Histotechnology I've had more than one pathologist tell me a monkey could do my job. Though one of them said it with a smile and added "a very highly skilled and well trained monkey", he was one of the few who knew better. How many of us monkeys have trained the whining and complaining residents how to do things correctly? Paula On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones wrote: > OMG Pam~ our pathologist said the exact same thing to us when we > started our Grossing training. > Sheesh. . . > Michael Ann > > > > > On 3/24/15, 11:52 AM, "Marcum, Pamela A" wrote: > > >That was nicer than the pathologist who told me years ago, "any > >monkey could be trained to do my job". I basically did not take the > >job I was interviewing for at the time. At least the next interview > >went a lot better and I did take the job. > > > >Pam > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > >Sanders, Jeanine (CDC/OID/NCEZID) > >Sent: Tuesday, March 24, 2015 12:30 PM > >To: Sue; Timothy Morken > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >Subject: RE: [Histonet] BS in Histotechnology > > > >I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can > >teach any trained dog how to section histology will never have the > >recognition those of us that have studied and trained deserve. > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue > >Sent: Tuesday, March 24, 2015 12:59 PM > >To: Timothy Morken > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >Subject: Re: [Histonet] BS in Histotechnology > > > >This is a fight that we continue to have with hospital administration. > >In my opinion histologists are just as important and needed as MT. > >Even though there is an increase in automation in pathology the hands > >on of a histologists is most important. The fact that hospital still > >consider a lower entry job is the reason there are not more of us. > >It is quite frustrating. > > > >Sue > >TJUH > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >--------------------------------------------------------------------- > >- Confidentiality Notice: This e-mail message, including any > >attachments, is for the sole use of the intended recipient(s) and may > >contain confidential and privileged information. Any unauthorized > >review, use, disclosure or distribution is prohibited. If you are not > >the intended recipient, please contact the sender by reply e-mail and > >destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garreyf <@t> gmail.com Tue Mar 24 17:33:41 2015 From: garreyf <@t> gmail.com (Garreyf) Date: Thu Apr 23 12:15:14 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <18D791D4EE07BC41BF05F8EF3CCCDE24678E4765@FTCSEAP4001.nam.int.local> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> <643626B74DE2814D8537057F40E1A10B15506756@CSI-MX-NODEA.CSI-LABS.local> <18D791D4EE07BC41BF05F8EF3CCCDE24678E4765@FTCSEAP4001.nam.int.local> Message-ID: I am trying to find (hire) a histotech and will make sure I don't use the words "trained monkey" in my interviews. . Ha ha. I truly value and appreciate a skilled and motivated histotech. I've had to train myself to cut my own sections in order to understand the process better. It is a great field; it's like art to me. There is no room for monkeys in my book. Garrey Sent from my iPhone > On Mar 24, 2015, at 5:22 PM, Stedman, Nancy wrote: > > As a pathologist I'd like to apologize for all the pathologists who have made comments like this.. forget trained monkeys and dogs, most (all?) pathologists cannot cut slides either, at least not slides they'd want to try to read. I know I can't. > > -Nancy Stedman > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner > Sent: Tuesday, March 24, 2015 4:26 PM > To: Paula Sicurello; Michael Ann Jones > Cc: histonet@lists.utsouthwestern.edu; Timothy Morken; Jennifer MacDonald; Marcum, Pamela A > Subject: RE: [Histonet] BS in Histotechnology > > I once worked with a Pathologist who said she was in a group meeting of other pathologists when one of them blurted out that a trained monkey could cut slides. My pathologist, having had the opportunity to review some cases from the offender's laboratory, promptly replied "Yes, and with the quality of your slides it looks like you did just that." She shut down the other pathologist really quickly, and as far as I know, we never received another case to review from him. My pathologist was not about to let that kind of arrogance stand. She was one of the best bosses I ever had! > > Mark Turner, Ph.D., HT(ASCP)QIHC > Manager, Histology/IHC > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > Sent: Tuesday, March 24, 2015 3:47 PM > To: Michael Ann Jones > Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, Pamela A; Timothy Morken > Subject: Re: [Histonet] BS in Histotechnology > > I've had more than one pathologist tell me a monkey could do my job. > Though one of them said it with a smile and added "a very highly skilled and well trained monkey", he was one of the few who knew better. > > How many of us monkeys have trained the whining and complaining residents how to do things correctly? > > Paula > > On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones > wrote: > >> OMG Pam~ our pathologist said the exact same thing to us when we >> started our Grossing training. >> Sheesh. . . >> Michael Ann >> >> >> >> >>> On 3/24/15, 11:52 AM, "Marcum, Pamela A" wrote: >>> >>> That was nicer than the pathologist who told me years ago, "any >>> monkey could be trained to do my job". I basically did not take the >>> job I was interviewing for at the time. At least the next interview >>> went a lot better and I did take the job. >>> >>> Pam >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >>> Sanders, Jeanine (CDC/OID/NCEZID) >>> Sent: Tuesday, March 24, 2015 12:30 PM >>> To: Sue; Timothy Morken >>> Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald >>> Subject: RE: [Histonet] BS in Histotechnology >>> >>> I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can >>> teach any trained dog how to section histology will never have the >>> recognition those of us that have studied and trained deserve. >>> >>> -----Original Message----- >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue >>> Sent: Tuesday, March 24, 2015 12:59 PM >>> To: Timothy Morken >>> Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald >>> Subject: Re: [Histonet] BS in Histotechnology >>> >>> This is a fight that we continue to have with hospital administration. >>> In my opinion histologists are just as important and needed as MT. >>> Even though there is an increase in automation in pathology the hands >>> on of a histologists is most important. The fact that hospital still >>> consider a lower entry job is the reason there are not more of us. >>> It is quite frustrating. >>> >>> Sue >>> TJUH >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> --------------------------------------------------------------------- >>> - Confidentiality Notice: This e-mail message, including any >>> attachments, is for the sole use of the intended recipient(s) and may >>> contain confidential and privileged information. Any unauthorized >>> review, use, disclosure or distribution is prohibited. If you are not >>> the intended recipient, please contact the sender by reply e-mail and >>> destroy all copies of the original message. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Tue Mar 24 17:38:50 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Thu Apr 23 12:15:17 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> <643626B74DE2814D8537057F40E1A10B15506756@CSI-MX-NODEA.CSI-LABS.local> <18D791D4EE07BC41BF05F8EF3CCCDE24678E4765@FTCSEAP4001.nam.int.local> Message-ID: It is an art! That's what I try to get across to those that I am teaching an mentoring. Think of every slide as an example of your skill and artistry. Also to think of every photo you take in EM as something that will be published. Besides, I like pretty colors much more than a blue line on a gel. :-) Paula On Tue, Mar 24, 2015 at 3:33 PM, Garreyf wrote: > I am trying to find (hire) a histotech and will make sure I don't use the > words "trained monkey" in my interviews. . Ha ha. > I truly value and appreciate a skilled and motivated histotech. I've had > to train myself to cut my own sections in order to understand the process > better. It is a great field; it's like art to me. There is no room for > monkeys in my book. > Garrey > > Sent from my iPhone > > > On Mar 24, 2015, at 5:22 PM, Stedman, Nancy < > Nancy.Stedman@buschgardens.com> wrote: > > > > As a pathologist I'd like to apologize for all the pathologists who have > made comments like this.. forget trained monkeys and dogs, most (all?) > pathologists cannot cut slides either, at least not slides they'd want to > try to read. I know I can't. > > > > -Nancy Stedman > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner > > Sent: Tuesday, March 24, 2015 4:26 PM > > To: Paula Sicurello; Michael Ann Jones > > Cc: histonet@lists.utsouthwestern.edu; Timothy Morken; Jennifer > MacDonald; Marcum, Pamela A > > Subject: RE: [Histonet] BS in Histotechnology > > > > I once worked with a Pathologist who said she was in a group meeting of > other pathologists when one of them blurted out that a trained monkey could > cut slides. My pathologist, having had the opportunity to review some > cases from the offender's laboratory, promptly replied "Yes, and with the > quality of your slides it looks like you did just that." She shut down the > other pathologist really quickly, and as far as I know, we never received > another case to review from him. My pathologist was not about to let that > kind of arrogance stand. She was one of the best bosses I ever had! > > > > Mark Turner, Ph.D., HT(ASCP)QIHC > > Manager, Histology/IHC > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello > > Sent: Tuesday, March 24, 2015 3:47 PM > > To: Michael Ann Jones > > Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, > Pamela A; Timothy Morken > > Subject: Re: [Histonet] BS in Histotechnology > > > > I've had more than one pathologist tell me a monkey could do my job. > > Though one of them said it with a smile and added "a very highly skilled > and well trained monkey", he was one of the few who knew better. > > > > How many of us monkeys have trained the whining and complaining > residents how to do things correctly? > > > > Paula > > > > On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones < > mjones@metropath.com> > > wrote: > > > >> OMG Pam~ our pathologist said the exact same thing to us when we > >> started our Grossing training. > >> Sheesh. . . > >> Michael Ann > >> > >> > >> > >> > >>> On 3/24/15, 11:52 AM, "Marcum, Pamela A" wrote: > >>> > >>> That was nicer than the pathologist who told me years ago, "any > >>> monkey could be trained to do my job". I basically did not take the > >>> job I was interviewing for at the time. At least the next interview > >>> went a lot better and I did take the job. > >>> > >>> Pam > >>> > >>> -----Original Message----- > >>> From: histonet-bounces@lists.utsouthwestern.edu > >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > >>> Sanders, Jeanine (CDC/OID/NCEZID) > >>> Sent: Tuesday, March 24, 2015 12:30 PM > >>> To: Sue; Timothy Morken > >>> Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >>> Subject: RE: [Histonet] BS in Histotechnology > >>> > >>> I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can > >>> teach any trained dog how to section histology will never have the > >>> recognition those of us that have studied and trained deserve. > >>> > >>> -----Original Message----- > >>> From: histonet-bounces@lists.utsouthwestern.edu > >>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue > >>> Sent: Tuesday, March 24, 2015 12:59 PM > >>> To: Timothy Morken > >>> Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >>> Subject: Re: [Histonet] BS in Histotechnology > >>> > >>> This is a fight that we continue to have with hospital administration. > >>> In my opinion histologists are just as important and needed as MT. > >>> Even though there is an increase in automation in pathology the hands > >>> on of a histologists is most important. The fact that hospital still > >>> consider a lower entry job is the reason there are not more of us. > >>> It is quite frustrating. > >>> > >>> Sue > >>> TJUH > >>> _______________________________________________ > >>> Histonet mailing list > >>> Histonet@lists.utsouthwestern.edu > >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >>> > >>> --------------------------------------------------------------------- > >>> - Confidentiality Notice: This e-mail message, including any > >>> attachments, is for the sole use of the intended recipient(s) and may > >>> contain confidential and privileged information. Any unauthorized > >>> review, use, disclosure or distribution is prohibited. If you are not > >>> the intended recipient, please contact the sender by reply e-mail and > >>> destroy all copies of the original message. > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From b427297 <@t> aol.com Tue Mar 24 19:06:01 2015 From: b427297 <@t> aol.com (William J. O'Connor III) Date: Thu Apr 23 12:15:21 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: Message-ID: <14c4e3ff9e7-7e25-24a3@webprd-m72.mail.aol.com> I have monkeys where I work, cynos. We tried to train them to not bite our fingers off - I wouldn't even try to teach them to use a microtome. -----Original Message----- From: Michael Ann Jones To: Stedman, Nancy ; Mark Turner ; Paula Sicurello Cc: histonet ; Timothy Morken ; Jennifer MacDonald ; Marcum, Pamela A Sent: Tue, Mar 24, 2015 4:30 pm Subject: Re: [Histonet] BS in Histotechnology Thanks Dr. Stedman! Good to hear! Michael Ann On 3/24/15, 3:22 PM, "Stedman, Nancy" wrote: >As a pathologist I'd like to apologize for all the pathologists who have >made comments like this.. forget trained monkeys and dogs, most (all?) >pathologists cannot cut slides either, at least not slides they'd want to >try to read. I know I can't. > >-Nancy Stedman > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark >Turner >Sent: Tuesday, March 24, 2015 4:26 PM >To: Paula Sicurello; Michael Ann Jones >Cc: histonet@lists.utsouthwestern.edu; Timothy Morken; Jennifer >MacDonald; Marcum, Pamela A >Subject: RE: [Histonet] BS in Histotechnology > >I once worked with a Pathologist who said she was in a group meeting of >other pathologists when one of them blurted out that a trained monkey >could cut slides. My pathologist, having had the opportunity to review >some cases from the offender's laboratory, promptly replied "Yes, and >with the quality of your slides it looks like you did just that." She >shut down the other pathologist really quickly, and as far as I know, we >never received another case to review from him. My pathologist was not >about to let that kind of arrogance stand. She was one of the best >bosses I ever had! > >Mark Turner, Ph.D., HT(ASCP)QIHC >Manager, Histology/IHC > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula >Sicurello >Sent: Tuesday, March 24, 2015 3:47 PM >To: Michael Ann Jones >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, Pamela >A; Timothy Morken >Subject: Re: [Histonet] BS in Histotechnology > >I've had more than one pathologist tell me a monkey could do my job. >Though one of them said it with a smile and added "a very highly skilled >and well trained monkey", he was one of the few who knew better. > >How many of us monkeys have trained the whining and complaining residents >how to do things correctly? > >Paula > >On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones >wrote: > >> OMG Pam~ our pathologist said the exact same thing to us when we >> started our Grossing training. >> Sheesh. . . >> Michael Ann >> >> >> >> >> On 3/24/15, 11:52 AM, "Marcum, Pamela A" wrote: >> >> >That was nicer than the pathologist who told me years ago, "any >> >monkey could be trained to do my job". I basically did not take the >> >job I was interviewing for at the time. At least the next interview >> >went a lot better and I did take the job. >> > >> >Pam >> > >> >-----Original Message----- >> >From: histonet-bounces@lists.utsouthwestern.edu >> >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> >Sanders, Jeanine (CDC/OID/NCEZID) >> >Sent: Tuesday, March 24, 2015 12:30 PM >> >To: Sue; Timothy Morken >> >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald >> >Subject: RE: [Histonet] BS in Histotechnology >> > >> >I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can >> >teach any trained dog how to section histology will never have the >> >recognition those of us that have studied and trained deserve. >> > >> >-----Original Message----- >> >From: histonet-bounces@lists.utsouthwestern.edu >> >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue >> >Sent: Tuesday, March 24, 2015 12:59 PM >> >To: Timothy Morken >> >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald >> >Subject: Re: [Histonet] BS in Histotechnology >> > >> >This is a fight that we continue to have with hospital administration. >> >In my opinion histologists are just as important and needed as MT. >> >Even though there is an increase in automation in pathology the hands >> >on of a histologists is most important. The fact that hospital still >> >consider a lower entry job is the reason there are not more of us. >> >It is quite frustrating. >> > >> >Sue >> >TJUH >> >_______________________________________________ >> >Histonet mailing list >> >Histonet@lists.utsouthwestern.edu >> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> >--------------------------------------------------------------------- >> >- Confidentiality Notice: This e-mail message, including any >> >attachments, is for the sole use of the intended recipient(s) and may >> >contain confidential and privileged information. Any unauthorized >> >review, use, disclosure or distribution is prohibited. If you are not >> >the intended recipient, please contact the sender by reply e-mail and >> >destroy all copies of the original message. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Tue Mar 24 20:46:06 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Thu Apr 23 12:15:24 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <14c4e3ff9e7-7e25-24a3@webprd-m72.mail.aol.com> References: <14c4e3ff9e7-7e25-24a3@webprd-m72.mail.aol.com> Message-ID: I tried to teach the starving graduate students the same things, with similar results :-) And it's not even Friday..... Paula On Tue, Mar 24, 2015 at 5:05 PM, William J. O'Connor III wrote: > I have monkeys where I work, cynos. We tried to train them to not bite > our fingers off - I wouldn't even try to teach them to use a microtome. > > > -----Original Message----- > From: Michael Ann Jones > To: Stedman, Nancy ; Mark Turner > ; Paula Sicurello > Cc: histonet ; Timothy Morken < > Timothy.Morken@ucsf.edu>; Jennifer MacDonald ; > Marcum, Pamela A > Sent: Tue, Mar 24, 2015 4:30 pm > Subject: Re: [Histonet] BS in Histotechnology > > Thanks Dr. Stedman! Good to hear! > Michael Ann > > > > > On 3/24/15, 3:22 PM, > "Stedman, Nancy" > wrote: > > >As a pathologist > I'd like to apologize for all the pathologists who have > >made comments like > this.. forget trained monkeys and dogs, most (all?) > >pathologists cannot cut > slides either, at least not slides they'd want to > >try to read. I know I > can't. > > > >-Nancy Stedman > > > > > > > > > >-----Original Message----- > >From:histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Mark > >Turner > >Sent: Tuesday, March 24, 2015 4:26 PM > >To: Paula > Sicurello; Michael Ann Jones > >Cc: histonet@lists.utsouthwestern.edu; Timothy > Morken; Jennifer > >MacDonald; Marcum, Pamela A > >Subject: RE: [Histonet] BS in > Histotechnology > > > >I once worked with a Pathologist who said she was in a > group meeting of > >other pathologists when one of them blurted out that a > trained monkey > >could cut slides. My pathologist, having had the opportunity > to review > >some cases from the offender's laboratory, promptly replied "Yes, > and > >with the quality of your slides it looks like you did just that." > She > >shut down the other pathologist really quickly, and as far as I know, > we > >never received another case to review from him. My pathologist was > not > >about to let that kind of arrogance stand. She was one of the > best > >bosses I ever had! > > > >Mark Turner, Ph.D., HT(ASCP)QIHC > >Manager, > Histology/IHC > > > > > >-----Original Message----- > >From:histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Paula > >Sicurello > >Sent: Tuesday, March 24, 2015 3:47 PM > >To: > Michael Ann Jones > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; > Marcum, Pamela > >A; Timothy Morken > >Subject: Re: [Histonet] BS in > Histotechnology > > > >I've had more than one pathologist tell me a monkey could > do my job. > >Though one of them said it with a smile and added "a very highly > skilled > >and well trained monkey", he was one of the few who knew > better. > > > >How many of us monkeys have trained the whining and complaining > residents > >how to do things correctly? > > > >Paula > > > >On Tue, Mar 24, 2015 at > 12:29 PM, Michael Ann Jones > >wrote: > > > >> OMG Pam~ our > pathologist said the exact same thing to us when we > >> started our Grossing > training. > >> Sheesh. . . > >> Michael Ann > >> > >> > >> > >> > >> On 3/24/15, 11:52 > AM, "Marcum, Pamela A" wrote: > >> > >> >That was nicer than > the pathologist who told me years ago, "any > >> >monkey could be trained to do > my job". I basically did not take the > >> >job I was interviewing for at the > time. At least the next interview > >> >went a lot better and I did take the > job. > >> > > >> >Pam > >> > > >> >-----Original Message----- > >> >From:histonet-bounces@lists.utsouthwestern.edu > >> > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > >> >Sanders, > Jeanine (CDC/OID/NCEZID) > >> >Sent: Tuesday, March 24, 2015 12:30 PM > >> >To: > Sue; Timothy Morken > >> >Cc: histonet@lists.utsouthwestern.edu; Jennifer > MacDonald > >> >Subject: RE: [Histonet] BS in Histotechnology > >> > > >> >I agree, > BUT>>>>>>>>>>>>>as long as many pathologists think you can > >> >teach any > trained dog how to section histology will never have the > >> >recognition those > of us that have studied and trained deserve. > >> > > >> >-----Original > Message----- > >> >From: histonet-bounces@lists.utsouthwestern.edu > >> > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue > >> >Sent: > Tuesday, March 24, 2015 12:59 PM > >> >To: Timothy Morken > >> >Cc:histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >> >Subject: Re: > [Histonet] BS in Histotechnology > >> > > >> >This is a fight that we continue to > have with hospital administration. > >> >In my opinion histologists are just as > important and needed as MT. > >> >Even though there is an increase in automation > in pathology the hands > >> >on of a histologists is most important. The fact > that hospital still > >> >consider a lower entry job is the reason there are not > more of us. > >> >It is quite frustrating. > >> > > >> >Sue > >> >TJUH > >> > >_______________________________________________ > >> >Histonet mailing list > >> > >Histonet@lists.utsouthwestern.edu > >> > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > >> > >--------------------------------------------------------------------- > >> >- > Confidentiality Notice: This e-mail message, including any > >> >attachments, is > for the sole use of the intended recipient(s) and may > >> >contain confidential > and privileged information. Any unauthorized > >> >review, use, disclosure or > distribution is prohibited. If you are not > >> >the intended recipient, please > contact the sender by reply e-mail and > >> >destroy all copies of the original > message. > >> > >> > >> _______________________________________________ > >> > Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >_______________________________________________ > >Histonet > mailing > list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet > mailing > listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From POWELL_SA <@t> mercer.edu Wed Mar 25 09:57:15 2015 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Thu Apr 23 12:15:27 2015 Subject: [Histonet] BS in Histotechnology In-Reply-To: <18D791D4EE07BC41BF05F8EF3CCCDE24678E4765@FTCSEAP4001.nam.int.local> References: <761E2B5697F795489C8710BCC72141FF367FF028@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D9863863261651430E@LRGHEXVS1.practice.lrgh.org> <761E2B5697F795489C8710BCC72141FF367FF12F@ex07.net.ucsf.edu> <813587568.1581253.1427216360670.JavaMail.zimbra@comcast.net> <3B2CD438E1628A41BD687E98B963B78137F1800E@EMBX-CLFT4.cdc.gov> <6c53fb52c0344acabc12a9720bf304b2@MAIL13M2N2.ad.uams.edu> <643626B74DE2814D8537057F40E1A10B15506756@CSI-MX-NODEA.CSI-LABS.local> <18D791D4EE07BC41BF05F8EF3CCCDE24678E4765@FTCSEAP4001.nam.int.local> Message-ID: <9BF995BC0E47744E9673A41486E24EE25C0207212D@MERCERMAIL.MercerU.local> Thank you Dr. Stedman. I appreciate your response. I have been fortunate to work with mostly respectful and appreciative pathologists in my 53 (in July) years as a histotechnologist. But have met some who think more of themselves than they should. One more thing, there are thousands of histotechs grossing now, a task that was once only a pathologist's job. So I wonder if the offenders think that a monkey can do their job? Just a thought. Thank you again for being a professionial. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stedman, Nancy Sent: Tuesday, March 24, 2015 5:22 PM To: Mark Turner; Paula Sicurello; Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Timothy Morken; Jennifer MacDonald; Marcum, Pamela A Subject: RE: [Histonet] BS in Histotechnology As a pathologist I'd like to apologize for all the pathologists who have made comments like this.. forget trained monkeys and dogs, most (all?) pathologists cannot cut slides either, at least not slides they'd want to try to read. I know I can't. -Nancy Stedman -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Tuesday, March 24, 2015 4:26 PM To: Paula Sicurello; Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Timothy Morken; Jennifer MacDonald; Marcum, Pamela A Subject: RE: [Histonet] BS in Histotechnology I once worked with a Pathologist who said she was in a group meeting of other pathologists when one of them blurted out that a trained monkey could cut slides. My pathologist, having had the opportunity to review some cases from the offender's laboratory, promptly replied "Yes, and with the quality of your slides it looks like you did just that." She shut down the other pathologist really quickly, and as far as I know, we never received another case to review from him. My pathologist was not about to let that kind of arrogance stand. She was one of the best bosses I ever had! Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, March 24, 2015 3:47 PM To: Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald; Marcum, Pamela A; Timothy Morken Subject: Re: [Histonet] BS in Histotechnology I've had more than one pathologist tell me a monkey could do my job. Though one of them said it with a smile and added "a very highly skilled and well trained monkey", he was one of the few who knew better. How many of us monkeys have trained the whining and complaining residents how to do things correctly? Paula On Tue, Mar 24, 2015 at 12:29 PM, Michael Ann Jones wrote: > OMG Pam~ our pathologist said the exact same thing to us when we > started our Grossing training. > Sheesh. . . > Michael Ann > > > > > On 3/24/15, 11:52 AM, "Marcum, Pamela A" wrote: > > >That was nicer than the pathologist who told me years ago, "any > >monkey could be trained to do my job". I basically did not take the > >job I was interviewing for at the time. At least the next interview > >went a lot better and I did take the job. > > > >Pam > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > >Sanders, Jeanine (CDC/OID/NCEZID) > >Sent: Tuesday, March 24, 2015 12:30 PM > >To: Sue; Timothy Morken > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >Subject: RE: [Histonet] BS in Histotechnology > > > >I agree, BUT>>>>>>>>>>>>>as long as many pathologists think you can > >teach any trained dog how to section histology will never have the > >recognition those of us that have studied and trained deserve. > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sue > >Sent: Tuesday, March 24, 2015 12:59 PM > >To: Timothy Morken > >Cc: histonet@lists.utsouthwestern.edu; Jennifer MacDonald > >Subject: Re: [Histonet] BS in Histotechnology > > > >This is a fight that we continue to have with hospital administration. > >In my opinion histologists are just as important and needed as MT. > >Even though there is an increase in automation in pathology the hands > >on of a histologists is most important. The fact that hospital still > >consider a lower entry job is the reason there are not more of us. > >It is quite frustrating. > > > >Sue > >TJUH > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >--------------------------------------------------------------------- > >- Confidentiality Notice: This e-mail message, including any > >attachments, is for the sole use of the intended recipient(s) and may > >contain confidential and privileged information. Any unauthorized > >review, use, disclosure or distribution is prohibited. If you are not > >the intended recipient, please contact the sender by reply e-mail and > >destroy all copies of the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From azdudley <@t> hotmail.com Tue Mar 31 10:44:50 2015 From: azdudley <@t> hotmail.com (anita) Date: Thu Apr 23 12:15:30 2015 Subject: [Histonet] unsubscribe Message-ID: