From j.benavides at eae.csic.es Mon Jun 1 06:28:43 2015 From: j.benavides at eae.csic.es (Julio Benavides) Date: Mon, 01 Jun 2015 13:28:43 +0200 Subject: [Histonet] PAS-diastase staining In-Reply-To: References: <55686B16.1060008@eae.csic.es> Message-ID: <20150601132843.Horde._Zn0MaB-ndqWiQRw_CWjLw2@webmail.csic.es> Hi, Sigma has discontinued selling type VIII-alfa amylase from barley (catalogue ref A2771). Anyone knows any other commercial source of this enzyme or if the other amylase (other types of alfa amylase or beta amylase) work for PAS-D? Thanks Julio Bea DeBrosse-Serra escribi?: > Hi Julio, > > Here is our procedure. It is very straight forward. > > Beatrice > > Beatrice DeBrosse-Serra HT(ASCP)QIHC > Isis Pharmaceuticals > Antisense Drug Discovery > 2855 Gazelle Ct. > Carlsbad, CA 92010 > 760-603-2371 > > > > > -----Original Message----- > From: Julio Benavides [mailto:j.benavides at eae.csic.es] > Sent: Friday, May 29, 2015 6:35 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] PAS-diastase staining > > Hi there, > > I am trying to do a PAA-diastase in formalin-fixed embedded samples > (ovine liver). I would be most grateful if someone could give me a > hand in: > > -Protocol (enzyme concentration, time of digestion...) for diastase > digestion (I am assuming that PAS afterwards is the normal protocol > for PAS) -A commercial source for diastase working in these samples. > Is the porcine amylase from sigma any good? cheaper options? > > As always, > > thank you very much for all your comments > > Cheers > > Julio > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.benavides at eae.csic.es Mon Jun 1 07:19:23 2015 From: j.benavides at eae.csic.es (Julio Benavides) Date: Mon, 01 Jun 2015 14:19:23 +0200 Subject: [Histonet] PAS-diastase staining In-Reply-To: <00cda6043e9e48a39db161ffdaa0433b@evcspmbx03.ads.northwestern.edu> References: <55686B16.1060008@eae.csic.es> <20150601132843.Horde._Zn0MaB-ndqWiQRw_CWjLw2@webmail.csic.es> <00cda6043e9e48a39db161ffdaa0433b@evcspmbx03.ads.northwestern.edu> Message-ID: <20150601141923.Horde.xkJw6GUxz_zSNdJxEHMj5w2@webmail.csic.es> Yes, they have, and it is a mixture of alfa and beta amylase, which I think should be better. Thanks a lot Bernice Julio Bernice Frederick escribi?: > Fisher should gave it. I know they have diastase of malt.... > > Bernice Frederick HTL (ASCP) > Senior Research Tech > Pathology Core Facility > Robert. H. Lurie Cancer Center > Northwestern University > 710 N Fairbanks Court > Olson 8-421 > Chicago,IL 60611 > 312-503-3723 > b-frederick at northwestern.edu > > > -----Original Message----- > From: Julio Benavides [mailto:j.benavides at eae.csic.es] > Sent: Monday, June 01, 2015 6:29 AM > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] PAS-diastase staining > > Hi, > > Sigma has discontinued selling type VIII-alfa amylase from barley > (catalogue ref A2771). Anyone knows any other commercial source of > this enzyme or if the other amylase (other types of alfa amylase or > beta amylase) work for PAS-D? > > Thanks > > > Julio > > Bea DeBrosse-Serra escribi?: > >> Hi Julio, >> >> Here is our procedure. It is very straight forward. >> >> Beatrice >> >> Beatrice DeBrosse-Serra HT(ASCP)QIHC >> Isis Pharmaceuticals >> Antisense Drug Discovery >> 2855 Gazelle Ct. >> Carlsbad, CA 92010 >> 760-603-2371 >> >> >> >> >> -----Original Message----- >> From: Julio Benavides [mailto:j.benavides at eae.csic.es] >> Sent: Friday, May 29, 2015 6:35 AM >> To: histonet at lists.utsouthwestern.edu >> Subject: [Histonet] PAS-diastase staining >> >> Hi there, >> >> I am trying to do a PAA-diastase in formalin-fixed embedded samples >> (ovine liver). I would be most grateful if someone could give me a >> hand in: >> >> -Protocol (enzyme concentration, time of digestion...) for diastase >> digestion (I am assuming that PAS afterwards is the normal protocol >> for PAS) -A commercial source for diastase working in these samples. >> Is the porcine amylase from sigma any good? cheaper options? >> >> As always, >> >> thank you very much for all your comments >> >> Cheers >> >> Julio >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting at geisinger.edu Mon Jun 1 09:58:41 2015 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Mon, 1 Jun 2015 14:58:41 +0000 Subject: [Histonet] plants in the lab In-Reply-To: References: Message-ID: So sad bc spider plants THRIVE ON the fumes!! -----Original Message----- From: Michelle Lamphere [mailto:MICHELLE.LAMPHERE at childrens.com] Sent: Sunday, May 31, 2015 8:36 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Our hospital Safety and Infection Control departments have policies in place prohibiting any potted plants from being in the hospital, anywhere. We can have them if they are only in water, but the soil presents an infection control issue for patients because of potential mildew, mold, spores, etc. Michelle Lamphere Senior Tech, Histology Anatomic Pathology O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere at childrens.com 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 Message: 2 Date: Fri, 29 May 2015 14:23:00 -0400 From: "Blazek, Linda" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] plants in the lab Message-ID: <5A2BD13465E061429D6455C8D6B40E3917421260A6 at IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Happy Friday all! Does anyone have documentation of the benefit of having plants in the lab? I know this was discusses quite a while ago but I can't find references for it. Any help would be appreciated. Thanks, Linda Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at privacy at childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From JWatson at gnf.org Mon Jun 1 10:32:37 2015 From: JWatson at gnf.org (James Watson) Date: Mon, 1 Jun 2015 15:32:37 +0000 Subject: [Histonet] plants in the lab In-Reply-To: References: Message-ID: http://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/19930073077.pdf James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson at gnf.org -----Original Message----- From: Bitting, Angela K. [mailto:akbitting at geisinger.edu] Sent: Monday, June 01, 2015 7:59 AM To: Michelle Lamphere; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab So sad bc spider plants THRIVE ON the fumes!! -----Original Message----- From: Michelle Lamphere [mailto:MICHELLE.LAMPHERE at childrens.com] Sent: Sunday, May 31, 2015 8:36 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Our hospital Safety and Infection Control departments have policies in place prohibiting any potted plants from being in the hospital, anywhere. We can have them if they are only in water, but the soil presents an infection control issue for patients because of potential mildew, mold, spores, etc. Michelle Lamphere Senior Tech, Histology Anatomic Pathology O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere at childrens.com 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 Message: 2 Date: Fri, 29 May 2015 14:23:00 -0400 From: "Blazek, Linda" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] plants in the lab Message-ID: <5A2BD13465E061429D6455C8D6B40E3917421260A6 at IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Happy Friday all! Does anyone have documentation of the benefit of having plants in the lab? I know this was discusses quite a while ago but I can't find references for it. Any help would be appreciated. Thanks, Linda Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at privacy at childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWatson at gnf.org Mon Jun 1 10:34:13 2015 From: JWatson at gnf.org (James Watson) Date: Mon, 1 Jun 2015 15:34:13 +0000 Subject: [Histonet] plants in the lab In-Reply-To: References: Message-ID: NASA did a lot of studies on this. http://en.wikipedia.org/wiki/NASA_Clean_Air_Study James Watson HT? ASCP GNF? Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel??? 858-332-4647 Fax?? 858-812-1915 jwatson at gnf.org -----Original Message----- From: Bitting, Angela K. [mailto:akbitting at geisinger.edu] Sent: Monday, June 01, 2015 7:59 AM To: Michelle Lamphere; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab So sad bc spider plants THRIVE ON the fumes!! -----Original Message----- From: Michelle Lamphere [mailto:MICHELLE.LAMPHERE at childrens.com] Sent: Sunday, May 31, 2015 8:36 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Our hospital Safety and Infection Control departments have policies in place prohibiting any potted plants from being in the hospital, anywhere. We can have them if they are only in water, but the soil presents an infection control issue for patients because of potential mildew, mold, spores, etc. Michelle Lamphere Senior Tech, Histology Anatomic Pathology O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere at childrens.com 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 Message: 2 Date: Fri, 29 May 2015 14:23:00 -0400 From: "Blazek, Linda" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] plants in the lab Message-ID: <5A2BD13465E061429D6455C8D6B40E3917421260A6 at IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Happy Friday all! Does anyone have documentation of the benefit of having plants in the lab? I know this was discusses quite a while ago but I can't find references for it. Any help would be appreciated. Thanks, Linda Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at privacy at childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting at geisinger.edu Mon Jun 1 11:17:46 2015 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Mon, 1 Jun 2015 16:17:46 +0000 Subject: [Histonet] TOMO IHC glass slides Message-ID: <7dc157b468544cdb88602a1fd2f78391@GHSEXMBX4W12V.geisinger.edu> Does anyone have any experience with using these slides on a Ventana platform? Thanks in advance, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From Timothy.Morken at ucsf.edu Mon Jun 1 15:40:31 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 1 Jun 2015 20:40:31 +0000 Subject: [Histonet] Patti Loykasek - k/L staining Message-ID: <761E2B5697F795489C8710BCC72141FF368379C5@ex07.net.ucsf.edu> Patti, do remember any details of the following procedure" We have a heart pathologist interested.... Thanks to you or anyone else that can help with this. ++++++++++++++ >From 2005... Kappa and lambda are certainly difficult to interpret. They both can be present circulating in the serum, thus the "background" staining in most tissue sections. We use polyclonal antibodies, and get quite good results. We use a tonsil as a control as bone marrows are just too precious & small for us to use. We look for the plasma cells to be staining with a dark cytoplasmic stain & a slight "blush" to the germinal centers of the tonsil. It does take a pathologist skilled in interpreting them. A true positive is in the cytoplasm of the cells, not in-between the cells (due to circulating K/L). We do use one titer for lymph nodes, and another for bone marrows & GI biopsies. Plus a different titer & pretreatment if we're looking for amyloid. Well, I hope I've helped & not confused you. I'd be happy to do my best to answer any questions that you have. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA +++++++++++++++++++++++++++++++++++++++++++++++++++ Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken at ucsfmedctr.org From faith14913 at aol.com Mon Jun 1 22:02:55 2015 From: faith14913 at aol.com (Mica) Date: Mon, 1 Jun 2015 22:02:55 -0500 Subject: [Histonet] PAS stain Message-ID: <16F1D792-CD79-458E-9D25-493D8BC0CE6C@aol.com> Just a question...what kind of hematoxylin do you use as a counter stain for your PAS stain and do you use a differentiator? Sent from my iPad From b-frederick at northwestern.edu Tue Jun 2 13:01:55 2015 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Tue, 2 Jun 2015 18:01:55 +0000 Subject: [Histonet] Toluidine blue Message-ID: <3832a339a14844f5b756b24765c52c13@evcspmbx03.ads.northwestern.edu> Hello all, I was taught to do Toluidine Blue O without a control. Is there actual one and what would it be? I'm staining a bone core. Don's ask why, it's research and what a researcher wants... Plus they have a protocol they are following for this cartilaganous defect. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu From TGoins at mt.gov Tue Jun 2 13:40:04 2015 From: TGoins at mt.gov (Goins, Tresa) Date: Tue, 2 Jun 2015 18:40:04 +0000 Subject: [Histonet] Toluidine blue In-Reply-To: <3832a339a14844f5b756b24765c52c13@evcspmbx03.ads.northwestern.edu> References: <3832a339a14844f5b756b24765c52c13@evcspmbx03.ads.northwestern.edu> Message-ID: We use a canine mast cell tumor as positive control - veterinary lab naturally. Probably looking for mast cells in the core. Tresa -----Original Message----- From: Bernice Frederick [mailto:b-frederick at northwestern.edu] Sent: Tuesday, June 02, 2015 12:02 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Toluidine blue Hello all, I was taught to do Toluidine Blue O without a control. Is there actual one and what would it be? I'm staining a bone core. Don's ask why, it's research and what a researcher wants... Plus they have a protocol they are following for this cartilaganous defect. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Tue Jun 2 15:50:06 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue, 2 Jun 2015 20:50:06 +0000 Subject: [Histonet] Toluidine blue In-Reply-To: <3832a339a14844f5b756b24765c52c13@evcspmbx03.ads.northwestern.edu> References: <3832a339a14844f5b756b24765c52c13@evcspmbx03.ads.northwestern.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53F1534@xmdb04.nch.kids> Hi Bernice, Your researcher probably has no idea. I can demonstrate cartilage, mast cells, bacteria and mucins depending on the tol blue technique I use. Ie not all tol blue techniques are the same. Ask him what he is after. Without control I would not recommend his work for publication if asked to review it. Lot of luck, Tony. ________________________________________ From: Bernice Frederick [b-frederick at northwestern.edu] Sent: Wednesday, 3 June 2015 4:01 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Toluidine blue Hello all, I was taught to do Toluidine Blue O without a control. Is there actual one and what would it be? I'm staining a bone core. Don's ask why, it's research and what a researcher wants... Plus they have a protocol they are following for this cartilaganous defect. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood at health.nsw.gov.au Tue Jun 2 16:02:46 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue, 2 Jun 2015 21:02:46 +0000 Subject: [Histonet] PAS stain In-Reply-To: <16F1D792-CD79-458E-9D25-493D8BC0CE6C@aol.com> References: <16F1D792-CD79-458E-9D25-493D8BC0CE6C@aol.com> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53F158C@xmdb04.nch.kids> Hi Mica, Most haematoxylins will do eg Harris or Mayer's. I would recommend a light counterstain (it is a counterstain, not the main stain). Differentiate if too strong. Also periodic acid treatment increases nuclear affinity for Hx so shorten the counterstain time. Regards, Tony ________________________________________ From: Mica [faith14913 at aol.com] Sent: Tuesday, 2 June 2015 1:02 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] PAS stain Just a question...what kind of hematoxylin do you use as a counter stain for your PAS stain and do you use a differentiator? Sent from my iPad _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood at health.nsw.gov.au Tue Jun 2 16:20:01 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue, 2 Jun 2015 21:20:01 +0000 Subject: [Histonet] plants in the lab In-Reply-To: References: , Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53F166C@xmdb04.nch.kids> Hi Michelle, Why would patients be in a histo lab anyway? ________________________________________ From: Michelle Lamphere [MICHELLE.LAMPHERE at childrens.com] Sent: Sunday, 31 May 2015 10:36 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Our hospital Safety and Infection Control departments have policies in place prohibiting any potted plants from being in the hospital, anywhere. We can have them if they are only in water, but the soil presents an infection control issue for patients because of potential mildew, mold, spores, etc. Michelle Lamphere Senior Tech, Histology Anatomic Pathology O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere at childrens.com 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 Message: 2 Date: Fri, 29 May 2015 14:23:00 -0400 From: "Blazek, Linda" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] plants in the lab Message-ID: <5A2BD13465E061429D6455C8D6B40E3917421260A6 at IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Happy Friday all! Does anyone have documentation of the benefit of having plants in the lab? I know this was discusses quite a while ago but I can't find references for it. Any help would be appreciated. Thanks, Linda Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at privacy at childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From gayle.callis at bresnan.net Tue Jun 2 17:38:50 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Tue, 2 Jun 2015 16:38:50 -0600 Subject: [Histonet] toluidine blue for cartilage with controls and mast cell staining Message-ID: <000001d09d84$e5a7fce0$b0f7f6a0$@bresnan.net> You wrote: We use a canine mast cell tumor as positive control - veterinary lab naturally. Probably looking for mast cells in the core. Tresa -----Original Message----- From: Bernice Frederick [mailto:b-frederick at northwestern.edu ] Sent: Tuesday, June 02, 2015 12:02 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Toluidine blue Hello all, I was taught to do Toluidine Blue O without a control. Is there actual one and what would it be? I'm staining a bone core. Don's ask why, it's research and what a researcher wants... Plus they have a protocol they are following for this cartilaganous defect. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 ************************************************************************* Bernice and Tresa, Having done a bone research study like this in the past, controls should be and were carefully done. You need to know normal cartilage from treated or defect in cartilage. The researcher certainly should have set their experiment up accordingly but may have controls in place now??? You did not say if this is articular cartilage from exterior joint surfaces where they took the core or deeper in the bone at the growth plate? These two cartilages will stain differently with T blue. Normally and when studying articular cartilage defects, it is wise to also do a safranin O/fast green stain along with the T Blue. Controls are extremely important and need to be carefully set up. Hopefully, you have a contralateral bone normal core from the same animal OR a core sample from an untreated, naive control animal. It was never stated what the experimental animal model is being used? I have done a study like this in the past. When core is decalcified with an acid or EDTA, then the control needs to be decalcified exactly the same way and at the same time as experimental cores with defect. If you are decalcifying with EDTA, then you should have a normal core that is not decalcified. This is difficult with mouse but possible with larger animals. The reason is to see if the proteoglycans in the articular or even the growth plate cartilage will be extracted by EDTA, and not appreciably by buffered formic acid. Articular cartilage where proteoglycans have been removed by a decalcifying agent will have different tinctorial quality (lighter) than cartilage never exposed to a decalcifying agent. EDTA is used by biochemists to extract proteoglycans for biochemical studies, and will the same thing in a cartilage section. Hence, there will be less staining seen with the toluidine blue or the Safranin O/fast green stain after EDTA. Hence you should run two controls, 1)a decalcified cartilage control and 2) an undecalcified control. How you decalcify will be important in order to retain proteoglycans in the cartilage. I strongly suggest using buffered formic acid, available commercially. You will find recipes for buffered formic acid in text books that contain sodium formate or sodium citrate. Look for these ingredients in product MSDS before you buy the formic acid decalcifying solutions. If there is any question about EDTA versus buffered formic acid and other acid decalcifiers i.e HCl, Nitric acid, etc. for cartilage studies, I will be happy to send publications concerning this topic privately. The Toluidine blue that we do for cartilage is designed to show cartilage staining and not mast cells. It could be the mast cells might be seen along with the cartilage staining but that is not the point. The toluidine blue stain I do for mast cells is entirely different from the toluidine blue cartilage staining protocol. I will be happy to send you a toluidine blue stain procedure for cartilage and also the SafO/Fast green protocol. I have a superb T blue mast cell stain from Churukian which allows mast cells to stand out without any blue background in surrounding tissues that is often seen with other T blue staining protocols. Hope this helps. Gayle M. Callis HTL/HT/MT(ASCP) From TGoins at mt.gov Wed Jun 3 09:18:19 2015 From: TGoins at mt.gov (Goins, Tresa) Date: Wed, 3 Jun 2015 14:18:19 +0000 Subject: [Histonet] plants in the lab In-Reply-To: <6D6BD1DE8A5571489398B392A38A7157F53F166C@xmdb04.nch.kids> References: , <6D6BD1DE8A5571489398B392A38A7157F53F166C@xmdb04.nch.kids> Message-ID: Patients do not have to go to the fungal spores, the spores will go to the patient. Depending on spore size, the spores may stay airborne for months - the spores "sediment" to a surface in still air. A condition not likely to occur in a hospital environment - they scurry around until finding a lung or mucous membrane to adhere to. It doesn't take long for a single miss-handled Aspergillus culture plate to contaminate an entire multi-story research lab. -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood at health.nsw.gov.au] Sent: Tuesday, June 02, 2015 3:20 PM To: Michelle Lamphere; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Hi Michelle, Why would patients be in a histo lab anyway? ________________________________________ From: Michelle Lamphere [MICHELLE.LAMPHERE at childrens.com] Sent: Sunday, 31 May 2015 10:36 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Our hospital Safety and Infection Control departments have policies in place prohibiting any potted plants from being in the hospital, anywhere. We can have them if they are only in water, but the soil presents an infection control issue for patients because of potential mildew, mold, spores, etc. Michelle Lamphere Senior Tech, Histology Anatomic Pathology O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere at childrens.com 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 Message: 2 Date: Fri, 29 May 2015 14:23:00 -0400 From: "Blazek, Linda" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] plants in the lab Message-ID: <5A2BD13465E061429D6455C8D6B40E3917421260A6 at IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Happy Friday all! Does anyone have documentation of the benefit of having plants in the lab? I know this was discusses quite a while ago but I can't find references for it. Any help would be appreciated. Thanks, Linda Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at privacy at childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cjbulmer104 at yahoo.com Wed Jun 3 10:35:15 2015 From: cjbulmer104 at yahoo.com (Cindy Bulmer) Date: Wed, 3 Jun 2015 15:35:15 +0000 (UTC) Subject: [Histonet] Patti Loykasek - k/L staining In-Reply-To: <761E2B5697F795489C8710BCC72141FF368379C5@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF368379C5@ex07.net.ucsf.edu> Message-ID: <1624282036.3894708.1433345715957.JavaMail.yahoo@mail.yahoo.com> Hi Tim,I use the Dako Kappa and Lambda, Poly Abs.? With the Kappa, my titer is 1:80,000 and the Lambda is 1:250,000.? I use the Innovex Rc Receptor Blocker as the diluent, this seems to cut down on a lot of the background.? My Pathologists are very happy with thesestains.? I use tonsil for my positive controls.Hope this helps, Cindy Bulmer HT(ASCP), QIHCIHC Supervisor, CTPLWaco, TX On Monday, June 1, 2015 3:43 PM, "Morken, Timothy" wrote: Patti, do remember any details of the following procedure" We have a heart pathologist? interested.... Thanks to you or anyone else that can help with this. ++++++++++++++ >From 2005... Kappa and lambda are certainly difficult to interpret. They both can be present circulating in the serum, thus the "background" staining in most tissue sections. We use polyclonal antibodies, and get quite good results. We use a tonsil as a control as bone marrows are just too precious & small for us to use. We look for the plasma cells to be staining with a dark cytoplasmic stain & a slight "blush" to the germinal centers of the tonsil. It does take a pathologist skilled in interpreting them. A true positive is in the cytoplasm of the cells, not in-between the cells (due to circulating K/L). We do use one titer for lymph nodes, and another for bone marrows & GI biopsies.? Plus a different titer & pretreatment if we're looking for amyloid. Well, I hope I've helped & not confused you. I'd be happy to do my best to answer any questions that you have. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA +++++++++++++++++++++++++++++++++++++++++++++++++++ Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken at ucsfmedctr.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer at mdanderson.org Wed Jun 3 10:39:19 2015 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Wed, 3 Jun 2015 15:39:19 +0000 Subject: [Histonet] PAS Stain Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC882249097E@D1PWPEXMBX05.mdanderson.edu> We use the hematoxylin that is used on the H&E stainer. While I know it is not optimal, the pathologists should be used to looking at it so it makes things a lot easier. The time is cut down to less than 30 seconds and we do not use a differentiator. For the students, it helps them to understand the versatility of the dye and its multiple uses. They don't get confused and are pleasantly surprised when they find out they can use another formulation while they are at their internships. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 ------------------------------ Message: 2 Date: Mon, 1 Jun 2015 22:02:55 -0500 From: Mica To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] PAS stain Message-ID: <16F1D792-CD79-458E-9D25-493D8BC0CE6C at aol.com> Content-Type: text/plain; charset=us-ascii Just a question...what kind of hematoxylin do you use as a counter stain for your PAS stain and do you use a differentiator? Sent from my iPad ------------------------------ From contact at excaliburpathology.com Wed Jun 3 10:42:39 2015 From: contact at excaliburpathology.com (Paula Pierce) Date: Wed, 3 Jun 2015 15:42:39 +0000 (UTC) Subject: [Histonet] plants in the lab In-Reply-To: References: Message-ID: <1160023190.5188125.1433346159417.JavaMail.yahoo@mail.yahoo.com> Ugh. TOO MANY REGULATIONS! What about plants and flowers taken to patient's rooms as get well wishes!?! Soil on shoes? Incoming air every time the front doors open an infinite number of times a day? Boxes supplies come in? Have you ever seen inside a semi truck trailer? The multiple holding docks boxes sit on awaiting transport? We cannot live in a bubble. ?Paula Pierce, BS, HTL(ASCP)HT?President?Excalibur Pathology, Inc.?5830 N Blue Lake Dr.?Norman, OK 73069?405-759-3953 PH?405-759-7513 FAX?www.excaliburpathology.com From: "Goins, Tresa" To: Tony Henwood (SCHN) ; Michelle Lamphere ; "'histonet at lists.utsouthwestern.edu'" Sent: Wednesday, June 3, 2015 9:18 AM Subject: Re: [Histonet] plants in the lab Patients do not have to go to the fungal spores, the spores will go to the patient. Depending on spore size, the spores may stay airborne for months - the spores "sediment" to a surface in still air. A condition not likely to occur in a hospital environment - they scurry around until finding a lung or mucous membrane to adhere to. It doesn't take long for a single miss-handled Aspergillus culture plate to contaminate an entire multi-story research lab. -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood at health.nsw.gov.au] Sent: Tuesday, June 02, 2015 3:20 PM To: Michelle Lamphere; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Hi Michelle, Why would patients be in a histo lab anyway? ________________________________________ From: Michelle Lamphere [MICHELLE.LAMPHERE at childrens.com] Sent: Sunday, 31 May 2015 10:36 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Our hospital Safety and Infection Control departments have policies in place prohibiting any potted plants from being in the hospital, anywhere.? We can have them if they are only in water, but the soil presents an infection control issue for patients because of potential mildew, mold, spores, etc. Michelle Lamphere Senior Tech, Histology Anatomic Pathology O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere at childrens.com 1935 Medical District Drive | B1.06? | Dallas, Texas? 75235 Message: 2 Date: Fri, 29 May 2015 14:23:00 -0400 From: "Blazek, Linda" To: "histonet at lists.utsouthwestern.edu" ? ? ? ? Subject: [Histonet] plants in the lab Message-ID: ? ? ? ? <5A2BD13465E061429D6455C8D6B40E3917421260A6 at IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Happy Friday all! Does anyone have documentation of the benefit of having plants in the lab?? I know this was discusses quite a while ago but I can't find references for it.? Any help would be appreciated. Thanks, Linda Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at privacy at childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mills at 3scan.com Wed Jun 3 11:44:21 2015 From: mills at 3scan.com (Caroline Miller) Date: Wed, 3 Jun 2015 09:44:21 -0700 Subject: [Histonet] plants in the lab In-Reply-To: <1160023190.5188125.1433346159417.JavaMail.yahoo@mail.yahoo.com> References: <1160023190.5188125.1433346159417.JavaMail.yahoo@mail.yahoo.com> Message-ID: <8FC0BCD0-1B39-4208-9836-CEE0A118B6E0@3scan.com> yes to all Paula's points! Plants make people happy and NASA has proved they are also very good for protecting our people in the lab. I just ordered 4 plants for our lab and I think other people should too! There is a point where regulation goes too far, and IMHO, not allowing plants in lab is a case in point Happy hump day everyone ? mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 > On Jun 3, 2015, at 8:42 AM, Paula Pierce wrote: > > Ugh. TOO MANY REGULATIONS! > What about plants and flowers taken to patient's rooms as get well wishes!?! > Soil on shoes? Incoming air every time the front doors open an infinite number of times a day? > Boxes supplies come in? Have you ever seen inside a semi truck trailer? The multiple holding docks boxes sit on awaiting transport? > We cannot live in a bubble. > > Paula Pierce, BS, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Norman, OK 73069 405-759-3953 PH 405-759-7513 FAX www.excaliburpathology.com > From: "Goins, Tresa" > To: Tony Henwood (SCHN) ; Michelle Lamphere ; "'histonet at lists.utsouthwestern.edu'" > Sent: Wednesday, June 3, 2015 9:18 AM > Subject: Re: [Histonet] plants in the lab > > Patients do not have to go to the fungal spores, the spores will go to the patient. > Depending on spore size, the spores may stay airborne for months - the spores "sediment" to a surface in still air. > A condition not likely to occur in a hospital environment - they scurry around until finding a lung or mucous membrane to adhere to. > It doesn't take long for a single miss-handled Aspergillus culture plate to contaminate an entire multi-story research lab. > > > > -----Original Message----- > From: Tony Henwood (SCHN) [mailto:tony.henwood at health.nsw.gov.au] > Sent: Tuesday, June 02, 2015 3:20 PM > To: Michelle Lamphere; 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] plants in the lab > > Hi Michelle, > Why would patients be in a histo lab anyway? > > ________________________________________ > From: Michelle Lamphere [MICHELLE.LAMPHERE at childrens.com] > Sent: Sunday, 31 May 2015 10:36 PM > To: 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] plants in the lab > > Our hospital Safety and Infection Control departments have policies in place prohibiting any potted plants from being in the hospital, anywhere. We can have them if they are only in water, but the soil presents an infection control issue for patients because of potential mildew, mold, spores, etc. > > > Michelle Lamphere > Senior Tech, Histology > Anatomic Pathology > O: 214.456.2318 | Fax: 214.456.0779 > E: michelle.lamphere at childrens.com > 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 > > > > Message: 2 > Date: Fri, 29 May 2015 14:23:00 -0400 > From: "Blazek, Linda" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] plants in the lab > Message-ID: > <5A2BD13465E061429D6455C8D6B40E3917421260A6 at IBMB7Exchange.digestivespecialists.com> > > Content-Type: text/plain; charset="us-ascii" > > Happy Friday all! > > Does anyone have documentation of the benefit of having plants in the lab? I know this was discusses quite a while ago but I can't find references for it. Any help would be appreciated. > > Thanks, > Linda > > > Please consider the environment before printing this e-mail > > This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the > individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at privacy at childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. > > This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************************* > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christina.wolfe at bms.com Wed Jun 3 12:45:32 2015 From: christina.wolfe at bms.com (Wolfe, Christina) Date: Wed, 3 Jun 2015 17:45:32 +0000 Subject: [Histonet] Movat's Pentachrome Paper? Message-ID: Hi all, Does anyone have a pdf of Movat's paper "Demonstration of All Connective Tissue Elements in a Single Section." Arch. Path. 60: pp 289-295 Thanks! Kristie Christina Wolfe, BSHA, MLT (ASCP), HT, QIHC Drug Safety Evaluation/Bristol-Myers Squibb Pathology Dept. 812-307-2093 ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From kgrobert at rci.rutgers.edu Wed Jun 3 13:02:21 2015 From: kgrobert at rci.rutgers.edu (Kathleen Roberts) Date: Wed, 3 Jun 2015 14:02:21 -0400 (EDT) Subject: [Histonet] Leica CV 5030 coverslipper and Fisherbrand Permount Message-ID: <506992064270513cced9c0bea5bae8ce.squirrel@webmail.rci.rutgers.edu> To all- We've been using Permount in our Leica cover slipper, but we have noticed that after a while, the Permount seems to get too thick and the machine doesn't dispense enough on the slides even after cleaning out the nozzle. Previously I just switched out the "old" Permount (saving it for manual cover slipping) for a new bottle, but has anyone tried to re-dilute it with toluene and put it back into the machine? If so, how do you do it? Thanks so much, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 From Richard.Cartun at hhchealth.org Wed Jun 3 14:39:28 2015 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Wed, 3 Jun 2015 19:39:28 +0000 Subject: [Histonet] Specimen Accessioning ..... Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E3EE9CA39@HHCEXCHMB03.hhcsystem.org> We have always accessioned specimens with multiple parts as 1, 2, 3, etc. and the corresponding paraffin blocks as A, B, C, etc. We are in the process of changing this to letters for the different specimen parts and numbers for the blocks. For those of you who do it this way, have you encountered any problems? For example, if the specimen arrives and the different parts are numbered, do you simply convert them to letters? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From mpence at grhs.net Wed Jun 3 14:52:02 2015 From: mpence at grhs.net (Mike Pence) Date: Wed, 3 Jun 2015 19:52:02 +0000 Subject: [Histonet] Specimen Accessioning ..... In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E3EE9CA39@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E3EE9CA39@HHCEXCHMB03.hhcsystem.org> Message-ID: All of our part types are A,B,C, etc. and the block count is 1,2,3 even if there is just one part and one block. Example: SB-15-12345 A1 -----Original Message----- From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] Sent: Wednesday, June 03, 2015 2:40 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Specimen Accessioning ..... We have always accessioned specimens with multiple parts as 1, 2, 3, etc. and the corresponding paraffin blocks as A, B, C, etc. We are in the process of changing this to letters for the different specimen parts and numbers for the blocks. For those of you who do it this way, have you encountered any problems? For example, if the specimen arrives and the different parts are numbered, do you simply convert them to letters? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum at uams.edu Wed Jun 3 15:07:57 2015 From: PAMarcum at uams.edu (Marcum, Pamela A) Date: Wed, 3 Jun 2015 20:07:57 +0000 Subject: [Histonet] Specimen Accessioning ..... In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E3EE9CA39@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E3EE9CA39@HHCEXCHMB03.hhcsystem.org> Message-ID: <08a0c39b9c6b444fb9b0af0b7cd6b981@MAIL13M2N1.ad.uams.edu> Most places I have been use the block as numeric and the part as alpha; 15SR1235 A (part) 1 (block) and etc. I have not seen it cause an issue and if we go beyond Z as the alpha then we double back to an AA for the part and so on. We just hope a resident doesn't go off track to often with the AA to whatever letter. Pam Marcum UAMS -----Original Message----- From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] Sent: Wednesday, June 03, 2015 2:39 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Specimen Accessioning ..... We have always accessioned specimens with multiple parts as 1, 2, 3, etc. and the corresponding paraffin blocks as A, B, C, etc. We are in the process of changing this to letters for the different specimen parts and numbers for the blocks. For those of you who do it this way, have you encountered any problems? For example, if the specimen arrives and the different parts are numbered, do you simply convert them to letters? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Joyce.Weems at emoryhealthcare.org Wed Jun 3 15:14:03 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Wed, 3 Jun 2015 20:14:03 +0000 Subject: [Histonet] Specimen Accessioning ..... In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E3EE9CA39@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E3EE9CA39@HHCEXCHMB03.hhcsystem.org> Message-ID: We do A, B, C, etc.. for the specimens. And then do A1, A2, A3, B1, B2, B3, etc... for the blocks. Took be a while to get them to change to that, but it is what makes sense to me... j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] Sent: Wednesday, June 03, 2015 3:39 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Specimen Accessioning ..... We have always accessioned specimens with multiple parts as 1, 2, 3, etc. and the corresponding paraffin blocks as A, B, C, etc. We are in the process of changing this to letters for the different specimen parts and numbers for the blocks. For those of you who do it this way, have you encountered any problems? For example, if the specimen arrives and the different parts are numbered, do you simply convert them to letters? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From blayjorge at gmail.com Wed Jun 3 15:18:05 2015 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Wed, 3 Jun 2015 16:18:05 -0400 Subject: [Histonet] Removing resin (EpoTek + hardener mix) from glass and metal Message-ID: Hello Histonetters: Do you know how to remove resin (EpoTek + hardener mix) from glass and metal. Droplets of resin + hardener have landed on a sensitive scale. If you have any constructive suggestions, please email me directly: blayjorge @ gmail.com Gratefully, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From Bonnie.Whitaker at osumc.edu Wed Jun 3 15:46:46 2015 From: Bonnie.Whitaker at osumc.edu (Whitaker, Bonnie) Date: Wed, 3 Jun 2015 16:46:46 -0400 Subject: [Histonet] Specimen Accessioning ..... In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E3EE9CA39@HHCEXCHMB03.hhcsystem.org> Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670E176B600E@EXMBOX-VP05.OSUMC.EDU> Ditto for me... most, if not all, of the places I've worked. Bonnie -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] Sent: Wednesday, June 03, 2015 4:14 PM To: 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Specimen Accessioning ..... We do A, B, C, etc.. for the specimens. And then do A1, A2, A3, B1, B2, B3, etc... for the blocks. Took be a while to get them to change to that, but it is what makes sense to me... j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] Sent: Wednesday, June 03, 2015 3:39 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Specimen Accessioning ..... We have always accessioned specimens with multiple parts as 1, 2, 3, etc. and the corresponding paraffin blocks as A, B, C, etc. We are in the process of changing this to letters for the different specimen parts and numbers for the blocks. For those of you who do it this way, have you encountered any problems? For example, if the specimen arrives and the different parts are numbered, do you simply convert them to letters? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=BueBtR5CdjFQiyIYzZtaWOofqRi54xSthMT548Y4JxA&s=IxKY4wM2DyHhBgoYLgk5UecAhPf2QOn2uQHXIgj__HQ&e= ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=BueBtR5CdjFQiyIYzZtaWOofqRi54xSthMT548Y4JxA&s=IxKY4wM2DyHhBgoYLgk5UecAhPf2QOn2uQHXIgj__HQ&e= From CBird at amli-denton.com Wed Jun 3 16:05:49 2015 From: CBird at amli-denton.com (Cindy Bird) Date: Wed, 3 Jun 2015 16:05:49 -0500 Subject: [Histonet] Specimen Accessioning ..... In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E3EE9CA39@HHCEXCHMB03.hhcsystem.org> Message-ID: We do the same in our lab Sent from my iPhone > On Jun 3, 2015, at 3:45 PM, Weems, Joyce K. wrote: > > We do A, B, C, etc.. for the specimens. > > And then do A1, A2, A3, B1, B2, B3, etc... for the blocks. Took be a while to get them to change to that, but it is what makes sense to me... j > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems at emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > -----Original Message----- > From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] > Sent: Wednesday, June 03, 2015 3:39 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Specimen Accessioning ..... > > We have always accessioned specimens with multiple parts as 1, 2, 3, etc. and the corresponding paraffin blocks as A, B, C, etc. We are in the process of changing this to letters for the different specimen parts and numbers for the blocks. For those of you who do it this way, have you encountered any problems? For example, if the specimen arrives and the different parts are numbered, do you simply convert them to letters? Thank you. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BDeBrosse-Serra at isisph.com Wed Jun 3 16:50:31 2015 From: BDeBrosse-Serra at isisph.com (Bea DeBrosse-Serra) Date: Wed, 3 Jun 2015 21:50:31 +0000 Subject: [Histonet] Specimen Accessioning ..... In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E3EE9CA39@HHCEXCHMB03.hhcsystem.org> Message-ID: Same here. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] Sent: Wednesday, June 03, 2015 1:14 PM To: 'Cartun, Richard'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Specimen Accessioning ..... We do A, B, C, etc.. for the specimens. And then do A1, A2, A3, B1, B2, B3, etc... for the blocks. Took be a while to get them to change to that, but it is what makes sense to me... j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] Sent: Wednesday, June 03, 2015 3:39 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Specimen Accessioning ..... We have always accessioned specimens with multiple parts as 1, 2, 3, etc. and the corresponding paraffin blocks as A, B, C, etc. We are in the process of changing this to letters for the different specimen parts and numbers for the blocks. For those of you who do it this way, have you encountered any problems? For example, if the specimen arrives and the different parts are numbered, do you simply convert them to letters? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.tolentino82 at gmail.com Wed Jun 3 16:50:40 2015 From: a.tolentino82 at gmail.com (Aimee Tolentino) Date: Wed, 3 Jun 2015 14:50:40 -0700 Subject: [Histonet] Specimen Accessioning ..... In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2E3EE9CA39@HHCEXCHMB03.hhcsystem.org> Message-ID: <3799D1CC-9C64-4D56-987D-9CA0108C5F52@gmail.com> Same in my lab Aimee Sent from my iPhone > On Jun 3, 2015, at 2:05 PM, Cindy Bird wrote: > > We do the same in our lab > > Sent from my iPhone > >> On Jun 3, 2015, at 3:45 PM, Weems, Joyce K. wrote: >> >> We do A, B, C, etc.. for the specimens. >> >> And then do A1, A2, A3, B1, B2, B3, etc... for the blocks. Took be a while to get them to change to that, but it is what makes sense to me... j >> >> Joyce Weems >> Pathology Manager >> 678-843-7376 Phone >> 678-843-7831 Fax >> joyce.weems at emoryhealthcare.org >> >> >> >> www.saintjosephsatlanta.org >> 5665 Peachtree Dunwoody Road >> Atlanta, GA 30342 >> >> This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. >> >> >> -----Original Message----- >> From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] >> Sent: Wednesday, June 03, 2015 3:39 PM >> To: histonet at lists.utsouthwestern.edu >> Subject: [Histonet] Specimen Accessioning ..... >> >> We have always accessioned specimens with multiple parts as 1, 2, 3, etc. and the corresponding paraffin blocks as A, B, C, etc. We are in the process of changing this to letters for the different specimen parts and numbers for the blocks. For those of you who do it this way, have you encountered any problems? For example, if the specimen arrives and the different parts are numbered, do you simply convert them to letters? Thank you. >> >> Richard >> >> Richard W. Cartun, MS, PhD >> Director, Histology & Immunopathology >> Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital >> 80 Seymour Street >> Hartford, CT 06102 >> (860) 972-1596 >> (860) 545-2204 Fax >> >> >> This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ________________________________ >> >> This e-mail message (including any attachments) is for the sole use of >> the intended recipient(s) and may contain confidential and privileged >> information. If the reader of this message is not the intended >> recipient, you are hereby notified that any dissemination, distribution >> or copying of this message (including any attachments) is strictly >> prohibited. >> >> If you have received this message in error, please contact >> the sender by reply e-mail message and destroy all copies of the >> original message (including attachments). >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garreyf at gmail.com Wed Jun 3 19:20:54 2015 From: garreyf at gmail.com (Garreyf) Date: Wed, 3 Jun 2015 20:20:54 -0400 Subject: [Histonet] Specimen Accessioning ..... In-Reply-To: <3799D1CC-9C64-4D56-987D-9CA0108C5F52@gmail.com> References: <9215BD4B0BA1B44D962A71C758B68D2E3EE9CA39@HHCEXCHMB03.hhcsystem.org> <3799D1CC-9C64-4D56-987D-9CA0108C5F52@gmail.com> Message-ID: <3235F979-2780-4EF3-8F90-62FCAD889374@gmail.com> Same here . Here is a summary of CAPs /NSH recommendations. http://www.cap.org/ShowProperty?nodePath=/UCMCon/Contribution%20Folders/WebContent/pdf/uniform-labeling-summary-of-recommendations.pdf If the link doesn't work, just google if you want. I guess you can do it either way. But, specimens designated as letters is most popular in my experience and the best way to do it in my opinion. Garrey Sent from my iPhone > On Jun 3, 2015, at 5:50 PM, Aimee Tolentino wrote: > > Same in my lab > Aimee > > Sent from my iPhone > >> On Jun 3, 2015, at 2:05 PM, Cindy Bird wrote: >> >> We do the same in our lab >> >> Sent from my iPhone >> >>> On Jun 3, 2015, at 3:45 PM, Weems, Joyce K. wrote: >>> >>> We do A, B, C, etc.. for the specimens. >>> >>> And then do A1, A2, A3, B1, B2, B3, etc... for the blocks. Took be a while to get them to change to that, but it is what makes sense to me... j >>> >>> Joyce Weems >>> Pathology Manager >>> 678-843-7376 Phone >>> 678-843-7831 Fax >>> joyce.weems at emoryhealthcare.org >>> >>> >>> >>> www.saintjosephsatlanta.org >>> 5665 Peachtree Dunwoody Road >>> Atlanta, GA 30342 >>> >>> This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. >>> >>> >>> -----Original Message----- >>> From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] >>> Sent: Wednesday, June 03, 2015 3:39 PM >>> To: histonet at lists.utsouthwestern.edu >>> Subject: [Histonet] Specimen Accessioning ..... >>> >>> We have always accessioned specimens with multiple parts as 1, 2, 3, etc. and the corresponding paraffin blocks as A, B, C, etc. We are in the process of changing this to letters for the different specimen parts and numbers for the blocks. For those of you who do it this way, have you encountered any problems? For example, if the specimen arrives and the different parts are numbered, do you simply convert them to letters? Thank you. >>> >>> Richard >>> >>> Richard W. Cartun, MS, PhD >>> Director, Histology & Immunopathology >>> Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital >>> 80 Seymour Street >>> Hartford, CT 06102 >>> (860) 972-1596 >>> (860) 545-2204 Fax >>> >>> >>> This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> ________________________________ >>> >>> This e-mail message (including any attachments) is for the sole use of >>> the intended recipient(s) and may contain confidential and privileged >>> information. If the reader of this message is not the intended >>> recipient, you are hereby notified that any dissemination, distribution >>> or copying of this message (including any attachments) is strictly >>> prohibited. >>> >>> If you have received this message in error, please contact >>> the sender by reply e-mail message and destroy all copies of the >>> original message (including attachments). >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKBoyd at chs.net Thu Jun 4 06:22:32 2015 From: DKBoyd at chs.net (Boyd, Debbie M) Date: Thu, 4 Jun 2015 11:22:32 +0000 Subject: [Histonet] [EXTERNAL] Re: plants in the lab In-Reply-To: <1160023190.5188125.1433346159417.JavaMail.yahoo@mail.yahoo.com> References: , <1160023190.5188125.1433346159417.JavaMail.yahoo@mail.yahoo.com> Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9F17850@TN001WEXMBX014.US.chs.net> There is a plethora of Joint Commission regulations concerning the below mentioned points of entry for microbacterial spores. If you are Joint Commissioned inspected, plants are not allowed in the lab. We are JC inspected and are not even allow to keep an exterior shipping package. If the box has a shipping label it has to be emptied and placed in plastic tubs. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Paula Pierce [contact at excaliburpathology.com] Sent: Wednesday, June 03, 2015 11:42 AM To: Goins, Tresa; Histonet Subject: [EXTERNAL] Re: [Histonet] plants in the lab Ugh. TOO MANY REGULATIONS! What about plants and flowers taken to patient's rooms as get well wishes!?! Soil on shoes? Incoming air every time the front doors open an infinite number of times a day? Boxes supplies come in? Have you ever seen inside a semi truck trailer? The multiple holding docks boxes sit on awaiting transport? We cannot live in a bubble. Paula Pierce, BS, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Norman, OK 73069 405-759-3953 PH 405-759-7513 FAX www.excaliburpathology.com From: "Goins, Tresa" To: Tony Henwood (SCHN) ; Michelle Lamphere ; "'histonet at lists.utsouthwestern.edu'" Sent: Wednesday, June 3, 2015 9:18 AM Subject: Re: [Histonet] plants in the lab Patients do not have to go to the fungal spores, the spores will go to the patient. Depending on spore size, the spores may stay airborne for months - the spores "sediment" to a surface in still air. A condition not likely to occur in a hospital environment - they scurry around until finding a lung or mucous membrane to adhere to. It doesn't take long for a single miss-handled Aspergillus culture plate to contaminate an entire multi-story research lab. -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood at health.nsw.gov.au] Sent: Tuesday, June 02, 2015 3:20 PM To: Michelle Lamphere; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Hi Michelle, Why would patients be in a histo lab anyway? ________________________________________ From: Michelle Lamphere [MICHELLE.LAMPHERE at childrens.com] Sent: Sunday, 31 May 2015 10:36 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Our hospital Safety and Infection Control departments have policies in place prohibiting any potted plants from being in the hospital, anywhere. We can have them if they are only in water, but the soil presents an infection control issue for patients because of potential mildew, mold, spores, etc. Michelle Lamphere Senior Tech, Histology Anatomic Pathology O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere at childrens.com 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 Message: 2 Date: Fri, 29 May 2015 14:23:00 -0400 From: "Blazek, Linda" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] plants in the lab Message-ID: <5A2BD13465E061429D6455C8D6B40E3917421260A6 at IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Happy Friday all! Does anyone have documentation of the benefit of having plants in the lab? I know this was discusses quite a while ago but I can't find references for it. Any help would be appreciated. Thanks, Linda Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at privacy at childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Dana.Spencer at vidanthealth.com Thu Jun 4 08:19:35 2015 From: Dana.Spencer at vidanthealth.com (Spencer, Dana) Date: Thu, 4 Jun 2015 13:19:35 +0000 Subject: [Histonet] IHC Billing Message-ID: <1433423976082.90126@vidanthealth.com> Does anyone have their IHC stain charges automatically dropping? We are currently manually entering the 88342 and 88341. The desire to automatically drop these charges has been mentioned by the pathologists. We have concerns that incorrect billing will be dropped based on the medicare rules. If you are automatically dropping these charges, how are you doing this? Thanks for your help! Dana ------------------------------------------------------------------------------ The contents of this e-mail (and any attachments) are confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. ============================================================================== From TGoins at mt.gov Thu Jun 4 08:56:34 2015 From: TGoins at mt.gov (Goins, Tresa) Date: Thu, 4 Jun 2015 13:56:34 +0000 Subject: [Histonet] Specimen Accessioning ..... In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E3EE9CA39@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E3EE9CA39@HHCEXCHMB03.hhcsystem.org> Message-ID: We ID the specimens with numbers OR letters to differentiate between "type" of sample received. The ID on the specimen workup sheet is always the same as the ID on the block and slide. A single large mass cut into multiple pieces is numbered 1, 2, etc. Different masses from a single accession are differentiated by letters; if large enough for multiple sections per mass, they are labeled A1, A2 etc. If we receive specimens with unique ID, e.g. cervical, thoracic and lumbar, we label C1, C2 . . T1, T2 . . etc. to eliminate the need for the pathologist to refer to the worksheet to determine which slides are which. Tresa -----Original Message----- From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] Sent: Wednesday, June 03, 2015 1:39 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Specimen Accessioning ..... We have always accessioned specimens with multiple parts as 1, 2, 3, etc. and the corresponding paraffin blocks as A, B, C, etc. We are in the process of changing this to letters for the different specimen parts and numbers for the blocks. For those of you who do it this way, have you encountered any problems? For example, if the specimen arrives and the different parts are numbered, do you simply convert them to letters? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joewalker at rrmc.org Thu Jun 4 10:20:41 2015 From: joewalker at rrmc.org (Joe W. Walker, Jr.) Date: Thu, 4 Jun 2015 15:20:41 +0000 Subject: [Histonet] IHC Billing In-Reply-To: <1433423976082.90126@vidanthealth.com> References: <1433423976082.90126@vidanthealth.com> Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC2981F61D@RRMBX03.rrmc.local> Hi Dana, We utilize Cerner as our LIS and hospital EMR. In our world, the IHC charge drops automatically. With that said, the "charge" that drops in our LIS module is actually a charge/task. This task is associated to an actual IHC charge in our billing/coding system. The billing system places the actual charge automatically based on what we send out of our LIS module. Our billing system has a "rule" set up that looks for Medicare patients and it then automatically transforms the IHC charge to the appropriate code for Medicare. In our LIS module, every IHC is associated with the 88342 charge, regardless if it is the first, second, third, etc, antibody on a specimen. The billing systems converts the second, third, etc to the 88141 automatically. The one catch to the rule is that we have to keep close track on specimens with multiple parts that also have multiple IHC's. In those instances, we have to manually adjust the IHC charge in order to ensure the correct numbers of 88342's and 88341's are being charged. It is a complicated process for us but it works for us. Success in your setting will obviously depend on the capabilities of your IT systems. Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker at rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: Spencer, Dana [mailto:Dana.Spencer at vidanthealth.com] Sent: Thursday, June 04, 2015 9:20 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC Billing Does anyone have their IHC stain charges automatically dropping? We are currently manually entering the 88342 and 88341. The desire to automatically drop these charges has been mentioned by the pathologists. We have concerns that incorrect billing will be dropped based on the medicare rules. If you are automatically dropping these charges, how are you doing this? Thanks for your help! Dana ------------------------------------------------------------------------------ The contents of this e-mail (and any attachments) are confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. ============================================================================== _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From madeathridge at pastnashville.com Fri Jun 5 07:12:58 2015 From: madeathridge at pastnashville.com (Maryann Deathridge) Date: Fri, 5 Jun 2015 05:12:58 -0700 Subject: [Histonet] Histotech position Message-ID: <41a32e993f134226a6e745424e329ecf@pastnashville.com> Have an opening for a part-time/ full-time histotechnologist in private pathology laboratory. Primarily 85% dermpath specimens, 15% AP specimens. Hours : 3am-11:30am. location: Nashville, Tn Contact email for more information if interested. madeathridge at pastnashville.com From akemiat3377 at gmail.com Fri Jun 5 07:59:44 2015 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Fri, 5 Jun 2015 05:59:44 -0700 Subject: [Histonet] Athena Healthcare computer system Message-ID: <9114F33E-BCA5-48B2-AE87-C679F3EC3A11@gmail.com> Good Morning and Happy Friday Histoland! Just curious if anyone out there is using the Athena Healthcare Computer system for your AP lab. If so, do have any special Macros you have created, or do you use a separate LIS system? I would greatly appreciate any feedback you can provide. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com Tele: (831) 375-3577 X117 From akemiat3377 at gmail.com Fri Jun 5 08:33:36 2015 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Fri, 5 Jun 2015 06:33:36 -0700 Subject: [Histonet] Athena Healthcare computer system Message-ID: <4B904D5E-B01E-4A6B-8000-50087E61B839@gmail.com> Good Morning and Happy Friday Histoland! Just curious if anyone out there is using the Athena Healthcare Computer system for your AP lab. If so, do you have any special Macros you have created, or do you use a separate LIS system? From my research, it appears to be a billing a patient scheduling system. I would greatly appreciate any feedback you can provide. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com Tele: (831) 375-3577 X117 From rennie1108 at yahoo.com Fri Jun 5 12:24:50 2015 From: rennie1108 at yahoo.com (Adrienne Anderson) Date: Fri, 5 Jun 2015 13:24:50 -0400 Subject: [Histonet] Decal of bone marrows Message-ID: <7C9E1D1E-6597-4123-86D2-3B9B65CEF25C@yahoo.com> Hi Histo-land, I have a really quick question: about how long does it take to decal a bone marrow biopsy? Thanks a bunch! Adrienne From Stacy_McLaughlin at cooley-dickinson.org Fri Jun 5 13:05:53 2015 From: Stacy_McLaughlin at cooley-dickinson.org (Stacy McLaughlin) Date: Fri, 5 Jun 2015 18:05:53 +0000 Subject: [Histonet] specimen jar opener Message-ID: Hello, We have a pathologist with a repetitive motion injury from opening the screw top specimen containers. Does anyone know of devices that would help with this? Happy Friday! Stacy Stacy McLaughlin, HT(ASCP) Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton,MA 01060 (413) 582-2019 Stacy_McLaughlin at Cooley-Dickinson.org From jpiche at wtbyhosp.org Fri Jun 5 13:09:26 2015 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Fri, 5 Jun 2015 18:09:26 +0000 Subject: [Histonet] Decal of bone marrows In-Reply-To: <7C9E1D1E-6597-4123-86D2-3B9B65CEF25C@yahoo.com> References: <7C9E1D1E-6597-4123-86D2-3B9B65CEF25C@yahoo.com> Message-ID: <631955447A364B45B9458D2905635110D91F260B@WIN08-MBX-01.wtbyhosp.org> Hi Adrienne, It all depends on what you use for decal. We use 5% Nitric acid for 1 hour or so. Sometimes it needs a bit more time. Have a good weekend! Jessica -----Original Message----- From: Adrienne Anderson [mailto:rennie1108 at yahoo.com] Sent: Friday, June 05, 2015 1:25 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Decal of bone marrows Hi Histo-land, I have a really quick question: about how long does it take to decal a bone marrow biopsy? Thanks a bunch! Adrienne _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From robert.jacox at thermofisher.com Fri Jun 5 13:26:45 2015 From: robert.jacox at thermofisher.com (Jacox, Robert A.) Date: Fri, 5 Jun 2015 11:26:45 -0700 Subject: [Histonet] specimen jar opener In-Reply-To: References: Message-ID: <7B380BF8E6354F4994F20E095D43F1710B0E167DA3@USPHO-MXVS01.amer.thermo.com> Good Grips make a jar opener that will handle up to a 480. Robert Jacox Manager, Global Tactical Marketing Anatomic Pathology Thermo Fisher Scientific Tel: 269-544-5651 l Mobile: 269-598-0747 robert.jacox at thermofisher.com l www.thermoscientific.com -----Original Message----- From: Stacy McLaughlin [mailto:Stacy_McLaughlin at cooley-dickinson.org] Sent: Friday, June 05, 2015 2:06 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] specimen jar opener Hello, We have a pathologist with a repetitive motion injury from opening the screw top specimen containers. Does anyone know of devices that would help with this? Happy Friday! Stacy Stacy McLaughlin, HT(ASCP) Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton,MA 01060 (413) 582-2019 Stacy_McLaughlin at Cooley-Dickinson.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rarbaugh at csdermatology.com Fri Jun 5 14:54:04 2015 From: rarbaugh at csdermatology.com (Arbaugh, Roberta) Date: Fri, 5 Jun 2015 19:54:04 +0000 Subject: [Histonet] Paraffin block disposal Message-ID: <469FD9C7F82DC749A414F241CB0474671FA2B3DB@EXCHANGE02.ohpin.com> Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them? DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. From rsimpkins at biocare.net Fri Jun 5 19:21:10 2015 From: rsimpkins at biocare.net (Robin Simpkins) Date: Sat, 6 Jun 2015 00:21:10 +0000 Subject: [Histonet] Histonet Digest, Vol 139, Issue 5 In-Reply-To: References: Message-ID: <6E8D3C52-364B-452A-90B4-BFB488BFE0D7@biocare.net> Robin Simpkins On Jun 5, 2015, at 10:19 AM, "histonet-request at lists.utsouthwestern.edu" wrote: Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Histotech position (Maryann Deathridge) 2. Athena Healthcare computer system (Eileen Akemi Allison) 3. Athena Healthcare computer system (Eileen Akemi Allison) ---------------------------------------------------------------------- Message: 1 Date: Fri, 5 Jun 2015 05:12:58 -0700 From: "Maryann Deathridge" To: Subject: [Histonet] Histotech position Message-ID: <41a32e993f134226a6e745424e329ecf at pastnashville.com> Content-Type: text/plain; charset="us-ascii" Have an opening for a part-time/ full-time histotechnologist in private pathology laboratory. Primarily 85% dermpath specimens, 15% AP specimens. Hours : 3am-11:30am. location: Nashville, Tn Contact email for more information if interested. madeathridge at pastnashville.com ------------------------------ Message: 2 Date: Fri, 5 Jun 2015 05:59:44 -0700 From: Eileen Akemi Allison To: Histonet Cc: aallison at Montereygi.com Subject: [Histonet] Athena Healthcare computer system Message-ID: <9114F33E-BCA5-48B2-AE87-C679F3EC3A11 at gmail.com> Content-Type: text/plain; charset=us-ascii Good Morning and Happy Friday Histoland! Just curious if anyone out there is using the Athena Healthcare Computer system for your AP lab. If so, do have any special Macros you have created, or do you use a separate LIS system? I would greatly appreciate any feedback you can provide. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com Tele: (831) 375-3577 X117 ------------------------------ Message: 3 Date: Fri, 5 Jun 2015 06:33:36 -0700 From: Eileen Akemi Allison To: Histonet Cc: aallison at Montereygi.com Subject: [Histonet] Athena Healthcare computer system Message-ID: <4B904D5E-B01E-4A6B-8000-50087E61B839 at gmail.com> Content-Type: text/plain; charset=us-ascii Good Morning and Happy Friday Histoland! Just curious if anyone out there is using the Athena Healthcare Computer system for your AP lab. If so, do you have any special Macros you have created, or do you use a separate LIS system? From my research, it appears to be a billing a patient scheduling system. I would greatly appreciate any feedback you can provide. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com Tele: (831) 375-3577 X117 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 139, Issue 5 **************************************** From pwnoyce at gmail.com Fri Jun 5 20:31:54 2015 From: pwnoyce at gmail.com (Peter Noyce) Date: Sat, 6 Jun 2015 11:31:54 +1000 Subject: [Histonet] tissue fixation-formaldehyde concentrations which is best. Message-ID: <000801d09ff8$94c245d0$be46d170$@gmail.com> Formaldehyde 37% (commonly called 100% formalin) compared to 4% ( commonly known as 10% neutral buffered formalin)-in theory the 37% should fix quicker and better BUT anecdotally it is said that high concentrations of formaldehyde quickly form a "shell" in the tissue and will stop good penetration and fixation to the deeper tissues AND over the years it has been said anecdotally that 4% concentration is the quickest and most complete for all sample (mammal and plant) fixation and preservation-are these true. Please do discuss the methanol or buffers that is in the formaldehyde, or discuss paraformaldehyde (which is non polymerized formaldehyde with no methanol, in water). Regards Peter Noyce PhD student. From jkiernan at uwo.ca Fri Jun 5 23:31:07 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Fri, 05 Jun 2015 23:31:07 -0500 Subject: [Histonet] tissue fixation-formaldehyde concentrations which is best. In-Reply-To: <7260fac225681.5572775a@uwo.ca> References: <000801d09ff8$94c245d0$be46d170$@gmail.com> <727098aa22d5b.55727498@uwo.ca> <7270e281272b7.557274d5@uwo.ca> <72809b2320fa2.55727513@uwo.ca> <73e086d92109d.55727541@uwo.ca> <7380fd2d215a8.55727622@uwo.ca> <7380cb1220cbe.55727661@uwo.ca> <73e0e56e2234a.5572769f@uwo.ca> <7280e43226b9f.557276dd@uwo.ca> <7340aeca22e6e.5572771c@uwo.ca> <7260fac225681.5572775a@uwo.ca> Message-ID: <7260ca6a26cb8.5572313b@uwo.ca> Dear Peter, Some of the information you mention as "anecdotal" is wrong. Formaldehyde and paraformaldehyde are well documented in original peer-reviewed papers and in all textbooks in the fields of histotechnology and histochemistry. Your anecdote about "high concentrations of formaldehyde quickly form a 'shell' in the tissue and will stop good penetration and fixation to the deeper tissues" has no basis in published work. Paraformaldehyde is an insoluble polymer, not "non polymerized formaldehyde". There is no such thing as "4% paraformaldehyde"! It is a sad fact that many labs do not contain even one book about histotechnology. Nearly all books in the field (and there are many) have plenty of references to review articles and original literature about the techniques. There are also several websites that provide links to useful papers. Check out some of the "useful links" on the Biological Stain Commission's site: http://biologicalstaincommission.org/useful-links/ As a graduate student, you need to work from primary sources or reliable secondary sources. When you defend your thesis, you won't want to justify your fixation or staining method by saying "I got the method by asking on an internet listserver". John Kiernan Professor Emeritus Anatomy & Cell Biology University of Western Ontario London,Canada = = = On 05/06/15, Peter Noyce wrote: > Formaldehyde 37% (commonly called 100% formalin) compared to 4% ( commonly > known as 10% neutral buffered formalin)-in theory the 37% should fix quicker > and better BUT anecdotally it is said that high concentrations of > formaldehyde quickly form a "shell" in the tissue and will stop good > penetration and fixation to the deeper tissues AND over the years it has > been said anecdotally that 4% concentration is the quickest and most > complete for all sample (mammal and plant) fixation and preservation-are > these true. Please do discuss the methanol or buffers that is in the > formaldehyde, or discuss paraformaldehyde (which is non polymerized > formaldehyde with no methanol, in water). > > Regards Peter Noyce PhD student. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From tony.henwood at health.nsw.gov.au Sat Jun 6 03:51:36 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sat, 6 Jun 2015 08:51:36 +0000 Subject: [Histonet] tissue fixation-formaldehyde concentrations which is best. In-Reply-To: <7260ca6a26cb8.5572313b@uwo.ca> References: <000801d09ff8$94c245d0$be46d170$@gmail.com> <727098aa22d5b.55727498@uwo.ca> <7270e281272b7.557274d5@uwo.ca> <72809b2320fa2.55727513@uwo.ca> <73e086d92109d.55727541@uwo.ca> <7380fd2d215a8.55727622@uwo.ca> <7380cb1220cbe.55727661@uwo.ca> <73e0e56e2234a.5572769f@uwo.ca> <7280e43226b9f.557276dd@uwo.ca> <7340aeca22e6e.5572771c@uwo.ca> <7260fac225681.5572775a@uwo.ca>, <7260ca6a26cb8.5572313b@uwo.ca> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53F2DE0@xmdb04.nch.kids> John, I totally agree Tony ________________________________________ From: John Kiernan [jkiernan at uwo.ca] Sent: Saturday, 6 June 2015 2:31 PM To: Peter Noyce; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] tissue fixation-formaldehyde concentrations which is best. Dear Peter, Some of the information you mention as "anecdotal" is wrong. Formaldehyde and paraformaldehyde are well documented in original peer-reviewed papers and in all textbooks in the fields of histotechnology and histochemistry. Your anecdote about "high concentrations of formaldehyde quickly form a 'shell' in the tissue and will stop good penetration and fixation to the deeper tissues" has no basis in published work. Paraformaldehyde is an insoluble polymer, not "non polymerized formaldehyde". There is no such thing as "4% paraformaldehyde"! It is a sad fact that many labs do not contain even one book about histotechnology. Nearly all books in the field (and there are many) have plenty of references to review articles and original literature about the techniques. There are also several websites that provide links to useful papers. Check out some of the "useful links" on the Biological Stain Commission's site: http://biologicalstaincommission.org/useful-links/ As a graduate student, you need to work from primary sources or reliable secondary sources. When you defend your thesis, you won't want to justify your fixation or staining method by saying "I got the method by asking on an internet listserver". John Kiernan Professor Emeritus Anatomy & Cell Biology University of Western Ontario London,Canada = = = On 05/06/15, Peter Noyce wrote: > Formaldehyde 37% (commonly called 100% formalin) compared to 4% ( commonly > known as 10% neutral buffered formalin)-in theory the 37% should fix quicker > and better BUT anecdotally it is said that high concentrations of > formaldehyde quickly form a "shell" in the tissue and will stop good > penetration and fixation to the deeper tissues AND over the years it has > been said anecdotally that 4% concentration is the quickest and most > complete for all sample (mammal and plant) fixation and preservation-are > these true. Please do discuss the methanol or buffers that is in the > formaldehyde, or discuss paraformaldehyde (which is non polymerized > formaldehyde with no methanol, in water). > > Regards Peter Noyce PhD student. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood at health.nsw.gov.au Sat Jun 6 04:15:07 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sat, 6 Jun 2015 09:15:07 +0000 Subject: [Histonet] [EXTERNAL] Re: plants in the lab In-Reply-To: <7EAFE982E328304DA6CE2B677BB76246A9F17850@TN001WEXMBX014.US.chs.net> References: , <1160023190.5188125.1433346159417.JavaMail.yahoo@mail.yahoo.com>, <7EAFE982E328304DA6CE2B677BB76246A9F17850@TN001WEXMBX014.US.chs.net> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53F2E90@xmdb04.nch.kids> I agree with Paula. The way you have explained the regs implies that even I would have problems entering the labs. Maybe ban people altogether and then we meet the regs. Lets keep it real and not fantasy. Tony ________________________________________ From: Boyd, Debbie M [DKBoyd at chs.net] Sent: Thursday, 4 June 2015 9:22 PM To: Paula Pierce; Goins, Tresa; Histonet Subject: Re: [Histonet] [EXTERNAL] Re: plants in the lab There is a plethora of Joint Commission regulations concerning the below mentioned points of entry for microbacterial spores. If you are Joint Commissioned inspected, plants are not allowed in the lab. We are JC inspected and are not even allow to keep an exterior shipping package. If the box has a shipping label it has to be emptied and placed in plastic tubs. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: Paula Pierce [contact at excaliburpathology.com] Sent: Wednesday, June 03, 2015 11:42 AM To: Goins, Tresa; Histonet Subject: [EXTERNAL] Re: [Histonet] plants in the lab Ugh. TOO MANY REGULATIONS! What about plants and flowers taken to patient's rooms as get well wishes!?! Soil on shoes? Incoming air every time the front doors open an infinite number of times a day? Boxes supplies come in? Have you ever seen inside a semi truck trailer? The multiple holding docks boxes sit on awaiting transport? We cannot live in a bubble. Paula Pierce, BS, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Norman, OK 73069 405-759-3953 PH 405-759-7513 FAX www.excaliburpathology.com From: "Goins, Tresa" To: Tony Henwood (SCHN) ; Michelle Lamphere ; "'histonet at lists.utsouthwestern.edu'" Sent: Wednesday, June 3, 2015 9:18 AM Subject: Re: [Histonet] plants in the lab Patients do not have to go to the fungal spores, the spores will go to the patient. Depending on spore size, the spores may stay airborne for months - the spores "sediment" to a surface in still air. A condition not likely to occur in a hospital environment - they scurry around until finding a lung or mucous membrane to adhere to. It doesn't take long for a single miss-handled Aspergillus culture plate to contaminate an entire multi-story research lab. -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood at health.nsw.gov.au] Sent: Tuesday, June 02, 2015 3:20 PM To: Michelle Lamphere; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Hi Michelle, Why would patients be in a histo lab anyway? ________________________________________ From: Michelle Lamphere [MICHELLE.LAMPHERE at childrens.com] Sent: Sunday, 31 May 2015 10:36 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Our hospital Safety and Infection Control departments have policies in place prohibiting any potted plants from being in the hospital, anywhere. We can have them if they are only in water, but the soil presents an infection control issue for patients because of potential mildew, mold, spores, etc. Michelle Lamphere Senior Tech, Histology Anatomic Pathology O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere at childrens.com 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 Message: 2 Date: Fri, 29 May 2015 14:23:00 -0400 From: "Blazek, Linda" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] plants in the lab Message-ID: <5A2BD13465E061429D6455C8D6B40E3917421260A6 at IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Happy Friday all! Does anyone have documentation of the benefit of having plants in the lab? I know this was discusses quite a while ago but I can't find references for it. Any help would be appreciated. Thanks, Linda Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at privacy at childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From tony.henwood at health.nsw.gov.au Sat Jun 6 04:35:24 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sat, 6 Jun 2015 09:35:24 +0000 Subject: [Histonet] plants in the lab In-Reply-To: References: , <6D6BD1DE8A5571489398B392A38A7157F53F166C@xmdb04.nch.kids>, Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53F2F32@xmdb04.nch.kids> Laboratory Air-conditioning systems should be separate from patient area systems (as is required for operating theatre units). I understand this to be good practice. ________________________________________ From: Goins, Tresa [TGoins at mt.gov] Sent: Thursday, 4 June 2015 12:18 AM To: Tony Henwood (SCHN); Michelle Lamphere; 'histonet at lists.utsouthwestern.edu' Subject: RE: plants in the lab Patients do not have to go to the fungal spores, the spores will go to the patient. Depending on spore size, the spores may stay airborne for months - the spores "sediment" to a surface in still air. A condition not likely to occur in a hospital environment - they scurry around until finding a lung or mucous membrane to adhere to. It doesn't take long for a single miss-handled Aspergillus culture plate to contaminate an entire multi-story research lab. -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood at health.nsw.gov.au] Sent: Tuesday, June 02, 2015 3:20 PM To: Michelle Lamphere; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Hi Michelle, Why would patients be in a histo lab anyway? ________________________________________ From: Michelle Lamphere [MICHELLE.LAMPHERE at childrens.com] Sent: Sunday, 31 May 2015 10:36 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Our hospital Safety and Infection Control departments have policies in place prohibiting any potted plants from being in the hospital, anywhere. We can have them if they are only in water, but the soil presents an infection control issue for patients because of potential mildew, mold, spores, etc. Michelle Lamphere Senior Tech, Histology Anatomic Pathology O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere at childrens.com 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 Message: 2 Date: Fri, 29 May 2015 14:23:00 -0400 From: "Blazek, Linda" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] plants in the lab Message-ID: <5A2BD13465E061429D6455C8D6B40E3917421260A6 at IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Happy Friday all! Does anyone have documentation of the benefit of having plants in the lab? I know this was discusses quite a while ago but I can't find references for it. Any help would be appreciated. Thanks, Linda Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at privacy at childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From garreyf at gmail.com Sat Jun 6 09:29:03 2015 From: garreyf at gmail.com (Garrey Faller) Date: Sat, 6 Jun 2015 10:29:03 -0400 Subject: [Histonet] Pre-floating tissue sections in dilute alcohol Message-ID: Hi everyone, I just became aware of this technique last week, and it seems to work great. I did a quick google search and found this quick reference. http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath? Its an extra step and introduces an extra chance to introduce floaters. But, the quality seems to be improved. Any thoughts? Thanks in advance. Garrey Faller Pathologist From a.tolentino82 at gmail.com Sat Jun 6 12:24:42 2015 From: a.tolentino82 at gmail.com (Aimee Tolentino) Date: Sat, 6 Jun 2015 10:24:42 -0700 Subject: [Histonet] Paraffin block disposal In-Reply-To: <469FD9C7F82DC749A414F241CB0474671FA2B3DB@EXCHANGE02.ohpin.com> References: <469FD9C7F82DC749A414F241CB0474671FA2B3DB@EXCHANGE02.ohpin.com> Message-ID: <1F36ABA5-452B-4556-8D1E-E5D09FDB22FC@gmail.com> That's a good question. I'd like to know the answer myself to that. :) Sent from my iPhone > On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta wrote: > > Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them? > > DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper at chla.usc.edu Sat Jun 6 13:34:17 2015 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Sat, 6 Jun 2015 18:34:17 +0000 Subject: [Histonet] Paraffin block disposal In-Reply-To: <1F36ABA5-452B-4556-8D1E-E5D09FDB22FC@gmail.com> References: <469FD9C7F82DC749A414F241CB0474671FA2B3DB@EXCHANGE02.ohpin.com>, <1F36ABA5-452B-4556-8D1E-E5D09FDB22FC@gmail.com> Message-ID: Hey Aimee, This has been discussed several times on Histonet. It sounds like it depends on the institution. Since they're FFPE, pathogens are not a concern. I didn't reply to all because someone will shout out, "What about CJD?" and then I would have to punch them. They should be able to go into the regular trash though, since there is nothing that anyone can catch from them. Here, just like Genzyme, we are told to dispose of them as regulated, biohazard waste. You would have PHI concerns if the patient's name is on them, so they'll need to be identified first . . . Thanks, Brian Cooper, HT (ASCP) Supervisor, Histology Children's Hospital, Los Angeles Sent from my Galaxy S5, so please forgive any weird typos . . . -----Original Message----- From: Aimee Tolentino [a.tolentino82 at gmail.com] Received: Saturday, 06 Jun 2015, 10:25AM To: Arbaugh, Roberta [rarbaugh at csdermatology.com] CC: histonet at lists.utsouthwestern.edu [histonet at lists.utsouthwestern.edu] Subject: Re: [Histonet] Paraffin block disposal That's a good question. I'd like to know the answer myself to that. :) Sent from my iPhone > On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta wrote: > > Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them? > > DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From carl.hobbs at kcl.ac.uk Sat Jun 6 14:08:47 2015 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sat, 6 Jun 2015 19:08:47 +0000 Subject: [Histonet] tissue fixation-formaldehyde concentrations Message-ID: I have to agree with the student, John. Sure, he is coming from ignorance ( not a bad situation: naivety is not a fault... we all are/were there at some point;-) Sure...I agree with you re the using of the word Paraformaldehyde as a fixative.... I often "sigh" when used. However, differential fixation ( zonal fixation) has always been an issue. We often see the zonal effects of this? Particularly in lymph nodes ( parenchymatous tissues) Obviously, when immersion fixing. Most often ...NOT with agitation. Respectfully, Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From brendal.finlay at medicalcenterclinic.com Sat Jun 6 15:29:18 2015 From: brendal.finlay at medicalcenterclinic.com (Brendal Finlay) Date: Sat, 06 Jun 2015 15:29:18 -0500 Subject: [Histonet] Paraffin block disposal References: <469FD9C7F82DC749A414F241CB0474671FA2B3DB@EXCHANGE02.ohpin.com>, <1F36ABA5-452B-4556-8D1E-E5D09FDB22FC@gmail.com> Message-ID: I agree with Brian, but we dispose of? blocks by treating them as regulated biohazard waste. We also?blocks them longer than 2 years.?The CLIA regulation states keeping them for a minimum of 2 years.?Outside facilities frequently request unstained slides or blocks?on?cases that are more than 2 years old.?Also, some patients require treatment for conditions for many years after the specimen is taken. If storage is not an issue,?keeping blocks?10 years (CAP requirements) is reasonable. ? Brendal C. Finlay, HT (ASCP) Senior Histologist Medical Center Clinic, P.A 8333 North Davis Highway Pensacola, FL 32514 Phone 850.474.8581 Fax 850.474.8584? -----Original Message----- From: "Cooper, Brian" To: a.tolentino82 at gmail.com Cc: histonet at lists.utsouthwestern.edu Date: 06/06/2015 13:34 Subject: Re: [Histonet] Paraffin block disposal Hey Aimee, This has been discussed several times on Histonet. It sounds like it depends on the institution. Since they're FFPE, pathogens are not a concern. I didn't reply to all because someone will shout out, "What about CJD?" and then I would have to punch them. They should be able to go into the regular trash though, since there is nothing that anyone can catch from them. Here, just like Genzyme, we are told to dispose of them as regulated, biohazard waste. You would have PHI concerns if the patient's name is on them, so they'll need to be identified first . . . Thanks, Brian Cooper, HT (ASCP) Supervisor, Histology Children's Hospital, Los Angeles Sent from my Galaxy S5, so please forgive any weird typos . . . -----Original Message----- From: Aimee Tolentino [a.tolentino82 at gmail.com] Received: Saturday, 06 Jun 2015, 10:25AM To: Arbaugh, Roberta [rarbaugh at csdermatology.com] CC: histonet at lists.utsouthwestern.edu [histonet at lists.utsouthwestern.edu] Subject: Re: [Histonet] Paraffin block disposal That's a good question. I'd like to know the answer myself to that. :) Sent from my iPhone > On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta wrote: > > Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them? > > DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. ? --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjfinney2010 at gmail.com Sat Jun 6 17:09:30 2015 From: tjfinney2010 at gmail.com (=?utf-8?B?dGpmaW5uZXkyMDEwQGdtYWlsLmNvbQ==?=) Date: Sat, 06 Jun 2015 15:09:30 -0700 (PDT) Subject: [Histonet] Pre-floating tissue sections in dilute alcohol Message-ID: <000f425a.2883754973a8f7e7@gmail.com> Yes I use it for brain. Happy Connecting. Sent from my Sprint Phone. ------ Original message------ From: Garrey Faller Date: Sat, Jun 6, 2015 10:48 AM To: histonet at lists.utsouthwestern.edu; Subject:[Histonet] Pre-floating tissue sections in dilute alcohol Hi everyone, I just became aware of this technique last week, and it seems to work great. I did a quick google search and found this quick reference. http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath? Its an extra step and introduces an extra chance to introduce floaters. But, the quality seems to be improved. Any thoughts? Thanks in advance. Garrey Faller Pathologist _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Sat Jun 6 18:19:53 2015 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 6 Jun 2015 19:19:53 -0400 Subject: [Histonet] Decalcification of bone marrows Message-ID: Adrienne (where?) asks: "I have a really quick question: about how long does it take to decal a bone marrow biopsy?" to which Jessica (where? - apparently in the US though) replies "It all depends on what you use for decal. We use 5% Nitric acid for 1 hour or so. Sometimes it needs a bit more time." To decalcify a Jamshidi needle bone marrow biopsy specimen in one of the ordinary commercial decalcifiers (usually hydrochloric acid, all deep dark trade secrets) takes about two hours. Nitric acid is NOT an acceptable decalcifier today, since it destroys immunoreactivity. I used to use it for most decalcification in the days before immunohistochemistry, but remember I've been practicing pathology for more than fifty years. Successful processing of bone marrow biopsy specimens requires cooperation among hematologist-oncologists, pathologists, and histotechnologists. (Dream on!) Practically speaking, you have to set a deadline - if your specimen arrives after 2 PM (for example) it doesn't get processed until tomorrow. Once again - Decal is the registered trademark of Decal Chemical Corporation's proprietary decalcifier. It is not a generic word for decalcifiers. (No, I don't work for them.) Bob Richmond Samurai Pathologist Maryville, TN From jmoreira at sidra.org Sat Jun 6 23:17:37 2015 From: jmoreira at sidra.org (Joana Moreira) Date: Sun, 7 Jun 2015 04:17:37 +0000 Subject: [Histonet] Pre-floating tissue sections in dilute alcohol Message-ID: I Garrey, I came across floating sections in dilute alcohol back in 2005 when I started working in the UK and have been using it ever since. I agree it introduces one more step and wouldn't use for every single section - there's no need. But I do find it very helpful with certain blocks/tissues - especially the very 'wrinkly' ones. When I started cutting during my training, we used other technique to avoid wrinkles (and waste of tissue sections) and produce 'perfect' sections. We used to have a small cold water bath to float and pick up the sections before transferring to the temperature controlled water bath. Joana Joana Moreira Supervisor - Anatomical Pathology Department of Pathology Sidra Medical & Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmoreira at sidra.org | www.sidra.org Message: 12 Date: Sat, 6 Jun 2015 10:29:03 -0400 From: Garrey Faller To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pre-floating tissue sections in dilute alcohol Message-ID: Content-Type: text/plain; charset=us-ascii Hi everyone, I just became aware of this technique last week, and it seems to work great. I did a quick google search and found this quick reference. http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath? Its an extra step and introduces an extra chance to introduce floaters. But, the quality seems to be improved. Any thoughts? Thanks in advance. Garrey Faller Pathologist ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 139, Issue 6 **************************************** Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. From gu.lang at gmx.at Sun Jun 7 05:38:43 2015 From: gu.lang at gmx.at (Gudrun Lang) Date: Sun, 7 Jun 2015 12:38:43 +0200 Subject: [Histonet] Pre-floating tissue sections in dilute alcohol In-Reply-To: References: Message-ID: <000001d0a10e$20f95690$62ec03b0$@gmx.at> Some of our histotechs use just cold tapwater for wrinkled sections before transferring them into the warm waterbath. I don't know, if the added ethanol makes any difference (maybe reducing surface tension). I would offer the possibility of using the additional waterbath, but let the histotechs decide, if it is necessary to use it. Gudrun Lang Leading histotech, Linz Austria -----Urspr?ngliche Nachricht----- Von: Garrey Faller [mailto:garreyf at gmail.com] Gesendet: Samstag, 06. Juni 2015 16:29 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Pre-floating tissue sections in dilute alcohol Hi everyone, I just became aware of this technique last week, and it seems to work great. I did a quick google search and found this quick reference. http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath? Its an extra step and introduces an extra chance to introduce floaters. But, the quality seems to be improved. Any thoughts? Thanks in advance. Garrey Faller Pathologist _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems at emoryhealthcare.org Mon Jun 8 07:48:12 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Mon, 8 Jun 2015 12:48:12 +0000 Subject: [Histonet] Pre-floating tissue sections in dilute alcohol In-Reply-To: <000001d0a10e$20f95690$62ec03b0$@gmx.at> References: <000001d0a10e$20f95690$62ec03b0$@gmx.at> Message-ID: I've never done two steps... just pour it in the water bath.. works great. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Gudrun Lang [mailto:gu.lang at gmx.at] Sent: Sunday, June 07, 2015 6:39 AM To: 'Garrey Faller' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pre-floating tissue sections in dilute alcohol Some of our histotechs use just cold tapwater for wrinkled sections before transferring them into the warm waterbath. I don't know, if the added ethanol makes any difference (maybe reducing surface tension). I would offer the possibility of using the additional waterbath, but let the histotechs decide, if it is necessary to use it. Gudrun Lang Leading histotech, Linz Austria -----Urspr?ngliche Nachricht----- Von: Garrey Faller [mailto:garreyf at gmail.com] Gesendet: Samstag, 06. Juni 2015 16:29 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Pre-floating tissue sections in dilute alcohol Hi everyone, I just became aware of this technique last week, and it seems to work great. I did a quick google search and found this quick reference. http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath? Its an extra step and introduces an extra chance to introduce floaters. But, the quality seems to be improved. Any thoughts? Thanks in advance. Garrey Faller Pathologist _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From TNMayer at mdanderson.org Mon Jun 8 09:01:34 2015 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Mon, 8 Jun 2015 14:01:34 +0000 Subject: [Histonet] : Paraffin block disposal Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC882249339F@D1PWPEXMBX05.mdanderson.edu> The regulation says two years. I was always led to believe that for Pedi, it should 10 years past the age of 18. Some facilities add the phrase 'past sign out' onto the policy for disposal. The methods can vary according to facility and state. In some places that could mean in the trash, in others biohazard waste. If confused check with another long standing facility and a newer one in the area to get an idea of what should be done. I have usually placed them in the biohazard trash, so that there would be no issues with anything. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 1 Date: Sat, 6 Jun 2015 10:24:42 -0700 From: Aimee Tolentino To: "Arbaugh, Roberta" Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Paraffin block disposal Message-ID: <1F36ABA5-452B-4556-8D1E-E5D09FDB22FC at gmail.com> Content-Type: text/plain; charset=us-ascii That's a good question. I'd like to know the answer myself to that. :) Sent from my iPhone > On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta wrote: > > Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them? > > DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From galinadeyneko at yahoo.com Mon Jun 8 09:10:04 2015 From: galinadeyneko at yahoo.com (Galina Deyneko) Date: Mon, 8 Jun 2015 14:10:04 +0000 (UTC) Subject: [Histonet] pre-floating tissue sections In-Reply-To: References: Message-ID: <43112806.8561113.1433772604763.JavaMail.yahoo@mail.yahoo.com> Hi Colleagues,?I also use this technique?? with intermediate bath with cold DI water and like it. also i breath on the surface of the block befor make one section, this helps making the section more smooth.?Galina DeynekoNovartis, Cambridge, MA?617-871-7613 w From: "histonet-request at lists.utsouthwestern.edu" To: histonet at lists.utsouthwestern.edu Sent: Sunday, June 7, 2015 1:00 PM Subject: Histonet Digest, Vol 139, Issue 7 Send Histonet mailing list submissions to ??? histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. Re: Paraffin block disposal (Aimee Tolentino) ? 2. Re: Paraffin block disposal (Cooper, Brian) ? 3. Re: tissue fixation-formaldehyde concentrations (Hobbs, Carl) ? 4. Re: Paraffin block disposal (Brendal Finlay) ? 5. Re: Pre-floating tissue sections in dilute alcohol ? ? ? (tjfinney2010 at gmail.com) ? 6. Re: Decalcification of bone marrows (Bob Richmond) ? 7. Re: Pre-floating tissue sections in dilute alcohol (Joana Moreira) ? 8. Re: Pre-floating tissue sections in dilute alcohol (Gudrun Lang) ---------------------------------------------------------------------- Message: 1 Date: Sat, 6 Jun 2015 10:24:42 -0700 From: Aimee Tolentino To: "Arbaugh, Roberta" Cc: "histonet at lists.utsouthwestern.edu" ??? Subject: Re: [Histonet] Paraffin block disposal Message-ID: <1F36ABA5-452B-4556-8D1E-E5D09FDB22FC at gmail.com> Content-Type: text/plain;??? charset=us-ascii That's a good question. I'd like to know the answer myself to that. :) Sent from my iPhone > On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta wrote: > > Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them? > > DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Sat, 6 Jun 2015 18:34:17 +0000 From: "Cooper, Brian" To: "a.tolentino82 at gmail.com" Cc: "histonet at lists.utsouthwestern.edu" ??? Subject: Re: [Histonet] Paraffin block disposal Message-ID: ??? Content-Type: text/plain; charset=us-ascii Hey Aimee, This has been discussed several times on Histonet. It sounds like it depends on the institution. Since they're FFPE, pathogens are not a concern. I didn't reply to all because someone will shout out, "What about CJD?" and then I would have to punch them. They should be able to go into the regular trash though, since there is nothing that anyone can catch from them. Here, just like Genzyme, we are told to dispose of them as regulated, biohazard waste. You would have PHI concerns if the patient's name is on them, so they'll need to be identified first . . . Thanks, Brian Cooper, HT (ASCP) Supervisor, Histology Children's Hospital, Los Angeles Sent from my Galaxy S5, so please forgive any weird typos . . . -----Original Message----- From: Aimee Tolentino [a.tolentino82 at gmail.com] Received: Saturday, 06 Jun 2015, 10:25AM To: Arbaugh, Roberta [rarbaugh at csdermatology.com] CC: histonet at lists.utsouthwestern.edu [histonet at lists.utsouthwestern.edu] Subject: Re: [Histonet] Paraffin block disposal That's a good question. I'd like to know the answer myself to that. :) Sent from my iPhone > On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta wrote: > > Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them? > > DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message.? --------------------------------------------------------------------- ------------------------------ Message: 3 Date: Sat, 6 Jun 2015 19:08:47 +0000 From: "Hobbs, Carl" To: histonet Subject: Re: [Histonet] tissue fixation-formaldehyde concentrations Message-ID: ??? ??? Content-Type: text/plain; charset="iso-8859-1" I have to agree with the student, John. Sure, he is coming from ignorance ( not a bad situation: naivety is not a fault...? we all are/were there at some point;-) Sure...I agree with you re the using of the word? Paraformaldehyde as a fixative.... I often "sigh" when used. However, differential fixation ( zonal fixation) has always been an issue. We often see the zonal effects of this? Particularly in lymph nodes ( parenchymatous tissues) Obviously, when immersion fixing. Most often ...NOT with agitation. Respectfully, Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813? ? ------------------------------ Message: 4 Date: Sat, 06 Jun 2015 15:29:18 -0500 From: "Brendal Finlay" To: a.tolentino82 at gmail.com,? Cooper, Brian Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin block disposal Message-ID: Content-Type: text/plain; charset=UTF-8 I agree with Brian, but we dispose of? blocks by treating them as regulated biohazard waste. We also?blocks them longer than 2 years.?The CLIA regulation states keeping them for a minimum of 2 years.?Outside facilities frequently request unstained slides or blocks?on?cases that are more than 2 years old.?Also, some patients require treatment for conditions for many years after the specimen is taken. If storage is not an issue,?keeping blocks?10 years (CAP requirements) is reasonable. ? Brendal C. Finlay, HT (ASCP) Senior Histologist Medical Center Clinic, P.A 8333 North Davis Highway Pensacola, FL 32514 Phone 850.474.8581 Fax 850.474.8584? -----Original Message----- From: "Cooper, Brian" To: a.tolentino82 at gmail.com Cc: histonet at lists.utsouthwestern.edu Date: 06/06/2015 13:34 Subject: Re: [Histonet] Paraffin block disposal Hey Aimee, This has been discussed several times on Histonet. It sounds like it depends on the institution. Since they're FFPE, pathogens are not a concern. I didn't reply to all because someone will shout out, "What about CJD?" and then I would have to punch them. They should be able to go into the regular trash though, since there is nothing that anyone can catch from them. Here, just like Genzyme, we are told to dispose of them as regulated, biohazard waste. You would have PHI concerns if the patient's name is on them, so they'll need to be identified first . . . Thanks, Brian Cooper, HT (ASCP) Supervisor, Histology Children's Hospital, Los Angeles Sent from my Galaxy S5, so please forgive any weird typos . . . -----Original Message----- From: Aimee Tolentino [a.tolentino82 at gmail.com] Received: Saturday, 06 Jun 2015, 10:25AM To: Arbaugh, Roberta [rarbaugh at csdermatology.com] CC: histonet at lists.utsouthwestern.edu [histonet at lists.utsouthwestern.edu] Subject: Re: [Histonet] Paraffin block disposal That's a good question. I'd like to know the answer myself to that. :) Sent from my iPhone > On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta wrote: > > Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them? > > DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. ? --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Sat, 06 Jun 2015 15:09:30 -0700 (PDT) From: tjfinney2010 at gmail.com To: garreyf at gmail.com , ??? histonet at lists.utsouthwestern.edu ??? Subject: Re: [Histonet] Pre-floating tissue sections in dilute alcohol Message-ID: <000f425a.2883754973a8f7e7 at gmail.com> Content-Type: text/plain;??? charset="utf-8" Yes I use it for brain.? Happy Connecting.? Sent from my Sprint Phone. ------ Original message------ From: Garrey Faller Date: Sat, Jun 6, 2015 10:48 AM To: histonet at lists.utsouthwestern.edu; Subject:[Histonet] Pre-floating tissue sections in dilute alcohol Hi everyone, I just became aware of this technique last week, and it seems to work great. I did a quick google search and found this quick reference. http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath? Its an extra step and introduces an extra chance to introduce floaters. But, the quality seems to be improved. Any thoughts?? Thanks in advance. Garrey Faller Pathologist _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Sat, 6 Jun 2015 19:19:53 -0400 From: Bob Richmond To: "Histonet at lists.utsouthwestern.edu" ??? Subject: Re: [Histonet] Decalcification of bone marrows Message-ID: ??? Content-Type: text/plain; charset=UTF-8 Adrienne (where?) asks: "I have a really quick question: about how long does it take to decal a bone marrow biopsy?" to which Jessica (where? - apparently in the US though) replies "It all depends on what you use for decal. We use 5% Nitric acid for 1 hour or so. Sometimes it needs a bit more time." To decalcify a Jamshidi needle bone marrow biopsy specimen in one of the ordinary commercial decalcifiers (usually hydrochloric acid, all deep dark trade secrets) takes about two hours. Nitric acid is NOT an acceptable decalcifier today, since it destroys immunoreactivity. I used to use it for most decalcification in the days before immunohistochemistry, but remember I've been practicing pathology for more than fifty years. Successful processing of bone marrow biopsy specimens requires cooperation among hematologist-oncologists, pathologists, and histotechnologists. (Dream on!) Practically speaking, you have to set a deadline - if your specimen arrives after 2 PM (for example) it doesn't get processed until tomorrow. Once again - Decal is the registered trademark of Decal Chemical Corporation's proprietary decalcifier. It is not a generic word for decalcifiers. (No, I don't work for them.) Bob Richmond Samurai Pathologist Maryville, TN ------------------------------ Message: 7 Date: Sun, 7 Jun 2015 04:17:37 +0000 From: Joana Moreira To: "histonet at lists.utsouthwestern.edu" ??? Subject: Re: [Histonet] Pre-floating tissue sections in dilute alcohol Message-ID: ??? ??? Content-Type: text/plain; charset="iso-8859-1" I Garrey, I came across floating sections in dilute alcohol back in 2005 when I started working in the UK and have been using it ever since. I agree it introduces one more step and wouldn't use for every single section - there's no need. But I do find it very helpful with certain blocks/tissues - especially the very 'wrinkly' ones. When I started cutting during my training, we used other technique to avoid wrinkles (and waste of tissue sections) and produce 'perfect' sections. We used to have a small cold water bath to float and pick up the sections before transferring to the temperature controlled water bath. Joana Joana Moreira Supervisor - Anatomical Pathology Department of Pathology Sidra Medical & Research Center PO Box 26999 | Doha, Qatar Direct Line? +974-4404-2036 jmoreira at sidra.org | www.sidra.org Message: 12 Date: Sat, 6 Jun 2015 10:29:03 -0400 From: Garrey Faller To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pre-floating tissue sections in dilute alcohol Message-ID: Content-Type: text/plain;? ? ? charset=us-ascii Hi everyone, I just became aware of this technique last week, and it seems to work great. I did a quick google search and found this quick reference. http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath? Its an extra step and introduces an extra chance to introduce floaters. But, the quality seems to be improved. Any thoughts? Thanks in advance. Garrey Faller Pathologist ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 139, Issue 6 **************************************** Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. ------------------------------ Message: 8 Date: Sun, 7 Jun 2015 12:38:43 +0200 From: "Gudrun Lang" To: "'Garrey Faller'" Cc: Subject: Re: [Histonet] Pre-floating tissue sections in dilute alcohol Message-ID: <000001d0a10e$20f95690$62ec03b0$@gmx.at> Content-Type: text/plain;??? charset="iso-8859-1" Some of our histotechs use just cold tapwater for wrinkled sections before transferring them? into the warm waterbath. I don't know, if the added ethanol makes any difference (maybe reducing surface tension). I would offer the possibility of using the additional waterbath, but let the histotechs decide, if it is necessary to use it. Gudrun Lang Leading histotech, Linz Austria -----Urspr?ngliche Nachricht----- Von: Garrey Faller [mailto:garreyf at gmail.com] Gesendet: Samstag, 06. Juni 2015 16:29 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Pre-floating tissue sections in dilute alcohol Hi everyone, I just became aware of this technique last week, and it seems to work great. I did a quick google search and found this quick reference. http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath? Its an extra step and introduces an extra chance to introduce floaters. But, the quality seems to be improved. Any thoughts?? Thanks in advance. Garrey Faller Pathologist _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 139, Issue 7 **************************************** From b-frederick at northwestern.edu Mon Jun 8 10:27:46 2015 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Mon, 8 Jun 2015 15:27:46 +0000 Subject: [Histonet] : Paraffin block disposal In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC882249339F@D1PWPEXMBX05.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC882249339F@D1PWPEXMBX05.mdanderson.edu> Message-ID: All, Any blocks for patients on clinical trials should also be kept to the end of the trial (please flag them and keep them somewhere) or sent to the cooperative group as the patient did consent for use of their blocks (usually) for future use. As well, one block is not always sufficient as that person may end up on more than one trial and need a different block (like a LN when a tumor block was originally needed). Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu -----Original Message----- From: Mayer,Toysha N [mailto:TNMayer at mdanderson.org] Sent: Monday, June 08, 2015 9:02 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] : Paraffin block disposal The regulation says two years. I was always led to believe that for Pedi, it should 10 years past the age of 18. Some facilities add the phrase 'past sign out' onto the policy for disposal. The methods can vary according to facility and state. In some places that could mean in the trash, in others biohazard waste. If confused check with another long standing facility and a newer one in the area to get an idea of what should be done. I have usually placed them in the biohazard trash, so that there would be no issues with anything. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 1 Date: Sat, 6 Jun 2015 10:24:42 -0700 From: Aimee Tolentino To: "Arbaugh, Roberta" Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Paraffin block disposal Message-ID: <1F36ABA5-452B-4556-8D1E-E5D09FDB22FC at gmail.com> Content-Type: text/plain; charset=us-ascii That's a good question. I'd like to know the answer myself to that. :) Sent from my iPhone > On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta wrote: > > Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them? > > DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper at chla.usc.edu Mon Jun 8 10:44:17 2015 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Mon, 8 Jun 2015 15:44:17 +0000 Subject: [Histonet] : Paraffin block disposal In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC882249339F@D1PWPEXMBX05.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC882249339F@D1PWPEXMBX05.mdanderson.edu> Message-ID: At this institution, we cite CAP's guidelines (ANP.12500) in our retention policy as a minimum of 10 year retention, prior to disposal of patient tissues. In practice we go further than that--we've never discarded a patient's blocks. The only thing we ever gotten rid of was animal research tissues, and even then, sparingly. They make great practice tissue blocks for histology students! Brian Cooper CHLA -----Original Message----- From: Mayer,Toysha N [mailto:TNMayer at mdanderson.org] Sent: Monday, June 08, 2015 7:02 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] : Paraffin block disposal The regulation says two years. I was always led to believe that for Pedi, it should 10 years past the age of 18. Some facilities add the phrase 'past sign out' onto the policy for disposal. The methods can vary according to facility and state. In some places that could mean in the trash, in others biohazard waste. If confused check with another long standing facility and a newer one in the area to get an idea of what should be done. I have usually placed them in the biohazard trash, so that there would be no issues with anything. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 1 Date: Sat, 6 Jun 2015 10:24:42 -0700 From: Aimee Tolentino To: "Arbaugh, Roberta" Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Paraffin block disposal Message-ID: <1F36ABA5-452B-4556-8D1E-E5D09FDB22FC at gmail.com> Content-Type: text/plain; charset=us-ascii That's a good question. I'd like to know the answer myself to that. :) Sent from my iPhone > On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta wrote: > > Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them? > > DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From blayjorge at gmail.com Mon Jun 8 18:34:32 2015 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Mon, 8 Jun 2015 19:34:32 -0400 Subject: [Histonet] General Genetics (lecture and lab textbooks) Message-ID: General Genetics (lecture and lab textbooks) Dear Histonet-Listers: I wish to know if you have recommendations of *excellent* textbooks (lecture and labs) for General Genetics for science majors. Although I just finished teaching an upper division Genetic Analysis course and I am well familiarized with what is available, I am not as familiarized with la creme de la creme for General Genetics. Ideally, I want a textbook that is (list is not comprehensive): 1. As inexpensive as possible (available online, softbound, etc.). 2. Easy to read, understand. and profusely illustrated. 3. Does not assume more than intro. biology (for majors) knowledge. 4. Emphasizes principles of genetics, model organisms, critical thinking, experimental design, ecological and conservation genetics, etc. 5. Has a generous companion packages (incl. PowerPoints, online quiz cartridges - not the ones that reside with the publishers but the ones than reside on campus only, etc.). For anything online, technology is as close to 100% reliable and intuitive as possible. 6. Does not shy away from math and stats (because I do not shy away from those either). 7. Is not divorced of the historical context of genetics. 8. Highlights the importance of genetics in modern life. 9. For labs, all of the above and not just the usual genetics exercises. I want a book that invites exploration and engages all of us in the field. Ideally, with a manual for s/he that may be helping with setting up the lab. If you have any feedback, please kindly send it directly to me, blayjorge at gmail.com With gratefulness, Jorge P.S Apologies for potential duplicate emails. Jorge A. Santiago-Blay, PhD blaypublishers.comblay.cfm From eca9 at georgetown.edu Tue Jun 9 07:55:30 2015 From: eca9 at georgetown.edu (Eva Permaul) Date: Tue, 9 Jun 2015 08:55:30 -0400 Subject: [Histonet] Ki67 antibody detecting mouse Message-ID: Good morning, We used to have a ki67 antibody that detected mouse that worked really well. It was made in rat. It is however now really weak. We have received two different lots and it is fading fast. Could anyone recommend one that works well for IHC-P on mouse tissues? We would prefer if it was not made in mouse. Rabbit, Rat or Goat would be the best. Also if it has been published it would be great to know. Thank you, Eva Georgetown University From mw at personifysearch.com Tue Jun 9 08:19:38 2015 From: mw at personifysearch.com (Matt Ward) Date: Tue, 9 Jun 2015 09:19:38 -0400 Subject: [Histonet] New Field Support/Applications Opportunity Message-ID: <60978377f98500fbcc316bb59a401b2b@mail.gmail.com> Good morning! A world leader in Histology & IHC has recently opened a new Field Technical Support opportunity covering the Southeast. The ideal candidate will have a strong background in IHC, be based in the Southeast (ideally Atlanta), and be open to travel. This role will be customer facing and offer a strong career track with a world leader. Please e-mail me directly if you would be interested in learning more ? mw at personifysearch.com. Thanks! Matt Matt Ward *Program Manager ? RPO* *Personify * 5020 Weston Parkway Suite 315 Cary NC 27513 Direct Line: (919) 459-3654 Toll Free: (800) 875-6188 ext. 103 www.personifysearch.com From kmilne at bccrc.ca Tue Jun 9 12:06:53 2015 From: kmilne at bccrc.ca (Katy Milne) Date: Tue, 9 Jun 2015 17:06:53 +0000 Subject: [Histonet] Ki67 antibody detecting mouse In-Reply-To: References: Message-ID: Hi Eva, The RbMAb SP6 clone works really well for mouse http://www.springbio.com/products/Ki-67-SP6-/M306 I'm sure it's probably been published with at some point, we've used it extensively in house with no problems. Katy ------------------------------ Message: 2 Date: Tue, 9 Jun 2015 08:55:30 -0400 From: Eva Permaul To: histonet Subject: [Histonet] Ki67 antibody detecting mouse Message-ID: Content-Type: text/plain; charset=UTF-8 Good morning, We used to have a ki67 antibody that detected mouse that worked really well. It was made in rat. It is however now really weak. We have received two different lots and it is fading fast. Could anyone recommend one that works well for IHC-P on mouse tissues? We would prefer if it was not made in mouse. Rabbit, Rat or Goat would be the best. Also if it has been published it would be great to know. Thank you, Eva Georgetown University From thoward at unm.edu Tue Jun 9 12:30:13 2015 From: thoward at unm.edu (Tamara Howard) Date: Tue, 9 Jun 2015 17:30:13 +0000 Subject: [Histonet] Ki67 antibody Message-ID: Eva - We use the SP6 rabbit monoclonal, formerly from NeoMarkers - they now belong to Thermo. Several companies sell this clone; I know AbCam also has it. It does require heat-mediated AR for FFPE tissues, but works beautifully in mouse & human; I don't think I've used it on rat. https://www.fishersci.com/shop/products/anti-ki-67-clone-sp6-thermo-scientific-lab-vision/p-3139002 Tamara ................................................... Tamara Howard Dept. of Cell Biology & Physiology University of New Mexico Albuquerque, NM From NMargaryan at luriechildrens.org Tue Jun 9 12:36:51 2015 From: NMargaryan at luriechildrens.org (Margaryan, Naira) Date: Tue, 9 Jun 2015 17:36:51 +0000 Subject: [Histonet] Ki67 antibody In-Reply-To: References: Message-ID: <34B2EDA118548A4EB35D6E650345BA6453E980F6@SV-EX11.childrensmemorial.org> Me too, love it! Naira Ranked nationally in all 10 pediatric specialties by U.S. News & World Report (LCHOC Ver 1.0) This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. (LCHOC VER 1.0) -----Original Message----- From: Tamara Howard [mailto:thoward at unm.edu] Sent: Tuesday, June 09, 2015 12:30 PM To: eca9 at georgetown.edu Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Ki67 antibody Eva - We use the SP6 rabbit monoclonal, formerly from NeoMarkers - they now belong to Thermo. Several companies sell this clone; I know AbCam also has it. It does require heat-mediated AR for FFPE tissues, but works beautifully in mouse & human; I don't think I've used it on rat. https://www.fishersci.com/shop/products/anti-ki-67-clone-sp6-thermo-scientific-lab-vision/p-3139002 Tamara ................................................... Tamara Howard Dept. of Cell Biology & Physiology University of New Mexico Albuquerque, NM _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From careerstudio at bellsouth.net Tue Jun 9 15:50:34 2015 From: careerstudio at bellsouth.net (Barbara Siegel) Date: Tue, 9 Jun 2015 16:50:34 -0400 Subject: [Histonet] Histotechnologist career positions in Westchester, NY ~ Boston, MA ~ Charlotte, NC Message-ID: <004201d0a2f5$f021f970$d065ec50$@net> We have multiple clinical reference & hospital laboratory clients searching for Histotechnologists from minimum Associates degreed candidates who want to start their career in a mentoring environment to seasoned individuals. Westchester, New York NYS & ASCP license required. Tuesday - Saturday 2AM-10:30AM, 5 years exp within the past 10 years. Greater Boston, Massachusetts HT ASCP preferred or eligible, Mon-Fri, 2:00pm - 10:30pm Evening shift, 1-3 yrs exp, IHC exp preferred. Charlotte, North Carolina 2nd/3rd shift: 8pm - 4:30a.m. Sunday - Thursday. Entry level candidates are welcome in this location. Accountabilities will include performing routine & non-routine activities involved in the preparation of slides, for microscopic evaluation by pathologist(s): Ensure proper accessioning & labeling of all tissue samples, proper tissue processing & Embed processed tissue in paraffin. Process paperwork associated with accessioning & reporting. Prepare slides for routine Hematoxylin & Eosin staining & solutions & reagents for special stain procedures. Perform microtomy of embedded tissue, coverslipping of stained slides either manually or automated, special stain procedures, filing of finished blocks & slides.. as well as . routine maintenance & cleaning of equipment & troubleshoot minor equipment failures. Document remedial actions such as repairs & repeated tests. Adhere to laboratory's quality control policies, & document all quality control activities. Ensure all corporate safety, quality control & quality assurance standards are met, as well as compliance with all local, federal, CLIA & CAP regulations Please contact biolabcareers at aol.com for more information David King Career Studio 561-738-6363 www.linkedin.com/in/biotechnologyhires/ From THicks at gulfcoastdermpath.com Tue Jun 9 18:51:29 2015 From: THicks at gulfcoastdermpath.com (Trish Hicks) Date: Tue, 9 Jun 2015 23:51:29 +0000 Subject: [Histonet] LOINC Message-ID: <1C799C0A-1BA5-42CF-9550-31DFD99B3046@gulfcoastdermpath.com> Does anyone know what the LOINC codes are for the histology procedures that relate to 88305, 88312, 88313 and 88342 CPT codes? Are the LOINC codes based on the individual stain procedures or how are they configured? Thank you, Trish Hicks BS, HTL (ASCP) Gulf Coast Dermatopathology (813) 882-4206 CONFIDENTIALITY NOTICE: The contents of this email message and any attachments are intended solely for the addressee(s) and may contain confidential and/or privileged information and may be legally protected from disclosure. If you are not the intended recipient of this message or their agent, or if this message has been addressed to you in error, please immediately alert the sender by reply email and then delete this message and any attachments. If you are not the intended recipient, you are hereby notified that any use, dissemination, copying, or storage of this message or its attachments is strictly prohibited. From amaluenda at svi.edu.au Tue Jun 9 19:19:34 2015 From: amaluenda at svi.edu.au (Ana Maluenda) Date: Wed, 10 Jun 2015 10:19:34 +1000 (EST) Subject: [Histonet] Cryojane tape-transfer and hydration of sections In-Reply-To: <1782142376.21999.1433895338324.JavaMail.root@zstore.medstv.unimelb.edu.au> Message-ID: <1566232282.22052.1433895574036.JavaMail.root@zstore.medstv.unimelb.edu.au> Hello Histonetters! I was wondering if there is anyone out there who is currently using the CryoJane Tape-transfer system for bone sections. I have been trying to get good sections for mice tibia and femur and often find myself with problems. I spent a good time trying to improve the quality of sections (tried different temperatures, thicknesses and speed as well as 1x vs 4x coated slides). The sections improved (although not perfect), but I was planning to move on for a staining trial. Currently, the sections are taken at 5um, in a 4x coated slide, at -26oC. However, now I get myself with a problem when hydrating the sections. At the moment, once the sections are taken, they are kept in -20oC (for short-storage). They are then taken out and left at RT for 30 min before hydration with PBS for another 30 min. I have noticed that it seems as if the OCT around the sections doesn't completely dissolve. I have already tried PBS vs dH2O and played around with times (from 5 min to overnight) with no differences. Has anyone had this happening before? Can it be because of the 4x coat? Is there anything I can do to? And would this be a problem for immunofluorescence? Any advice would be much appreciated! Thanks in advance, Ana Ana Maluenda Research Assistant Stem Cell Regulation Unit From mbmphoto at gmail.com Tue Jun 9 22:28:27 2015 From: mbmphoto at gmail.com (Maria Mejia) Date: Tue, 9 Jun 2015 20:28:27 -0700 Subject: [Histonet] searching for a 50cm stainless steel profile A knife Message-ID: <4B6CD838-689A-4829-9274-ED3456A40FA6@gmail.com> Hello, Can any one tell me where I can buy a 50cm stainless steel profile A type knife to use on a tetrander cast iron sliding microtome? Any assistance from anyone will be greatly appreciated. Regards Maria Mejia UCSF Mission Bay San Francisco, CA From THicks at gulfcoastdermpath.com Wed Jun 10 05:54:24 2015 From: THicks at gulfcoastdermpath.com (Trish Hicks) Date: Wed, 10 Jun 2015 10:54:24 +0000 Subject: [Histonet] LOINC Codes Message-ID: <96D42D0E9FB88A46A1B3E3E82DE4CA9A43A8B65E@GCD-EXCH.tampa.gcd.local> Does anyone know what the LOINC codes are for the histology procedures that relate to 88305, 88312, 88313 and 88342 CPT codes? Are the LOINC codes based on the individual stain procedures or how are they configured? Thank You, Trish Hicks BS, HTL (ASCP) Laboratory Supervisor Gulf Coast Dermatopathology Laboratory, Inc. Ph: 813-882-4206 Fax: 813-886-0589 Toll Free: 877-654-7721 thicks at gulfcoastdermpath.com Confidentiality: The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of this information by persons or entities other than the intended recipient(s) is unauthorized and prohibited. Any transmission of confidential and/or privileged material to persons or entities other than the intended recipient(s) shall not be construed as a waiver of any privilege or confidence. If you receive this transmission in error, please contact the sender and delete the material. Virus Disclaimer: The contents of an attachment to this e-mail may contain software viruses, which could damage your computer system. We cannot accept liability for any damage you may sustain as a result of software viruses. You should carry out your own virus checks before opening any attachment. From akemiat3377 at gmail.com Wed Jun 10 07:25:10 2015 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Wed, 10 Jun 2015 05:25:10 -0700 Subject: [Histonet] searching for a 50cm stainless steel profile A knife In-Reply-To: <4B6CD838-689A-4829-9274-ED3456A40FA6@gmail.com> References: <4B6CD838-689A-4829-9274-ED3456A40FA6@gmail.com> Message-ID: <20DF08F4-5B22-4F56-9462-C11652AA1543@gmail.com> Maria: Hacker Instruments sells new and reconditioned knives. They also provide a knife sharpening service for a fee of course. I believe they have that size, but if not, they will help you. Here is their link. http://www.tidylabstore.com/newmikn.html Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com Tele: (831) 375-3577 X117 > On Jun 9, 2015, at 8:28 PM, Maria Mejia wrote: > > Hello, > > Can any one tell me where I can buy a 50cm stainless steel profile A type knife to use > on a tetrander cast iron sliding microtome? Any assistance from anyone will be > greatly appreciated. > > Regards > Maria Mejia > UCSF > Mission Bay > San Francisco, CA > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nto at stowers.org Wed Jun 10 07:31:58 2015 From: nto at stowers.org (Thomas, Nancy) Date: Wed, 10 Jun 2015 12:31:58 +0000 Subject: [Histonet] Cryojane tape-transfer and hydration of sections In-Reply-To: <1566232282.22052.1433895574036.JavaMail.root@zstore.medstv.unimelb.edu.au> References: <1782142376.21999.1433895338324.JavaMail.root@zstore.medstv.unimelb.edu.au> <1566232282.22052.1433895574036.JavaMail.root@zstore.medstv.unimelb.edu.au> Message-ID: <051e1bc8a82e4546a287f3486b9d3859@exchsrv2.sgc.loc> Ana, We once tried 4x coated slides thinking, like you, that it would deliver a better quality section and would stay on the slide better. Our researchers did not like them at all because of the high level of background staining and fluorescence. We only use 1x now. Nancy -----Original Message----- From: Ana Maluenda [mailto:amaluenda at svi.edu.au] Sent: Tuesday, June 09, 2015 7:20 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cryojane tape-transfer and hydration of sections Hello Histonetters! I was wondering if there is anyone out there who is currently using the CryoJane Tape-transfer system for bone sections. I have been trying to get good sections for mice tibia and femur and often find myself with problems. I spent a good time trying to improve the quality of sections (tried different temperatures, thicknesses and speed as well as 1x vs 4x coated slides). The sections improved (although not perfect), but I was planning to move on for a staining trial. Currently, the sections are taken at 5um, in a 4x coated slide, at -26oC. However, now I get myself with a problem when hydrating the sections. At the moment, once the sections are taken, they are kept in -20oC (for short-storage). They are then taken out and left at RT for 30 min before hydration with PBS for another 30 min. I have noticed that it seems as if the OCT around the sections doesn't completely dissolve. I have already tried PBS vs dH2O and played around with times (from 5 min to overnight) with no differences. Has anyone had this happening before? Can it be because of the 4x coat? Is there anything I can do to? And would this be a problem for immunofluorescence? Any advice would be much appreciated! Thanks in advance, Ana Ana Maluenda Research Assistant Stem Cell Regulation Unit _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum at uams.edu Wed Jun 10 08:05:10 2015 From: PAMarcum at uams.edu (Marcum, Pamela A) Date: Wed, 10 Jun 2015 13:05:10 +0000 Subject: [Histonet] searching for a 50cm stainless steel profile A knife In-Reply-To: <20DF08F4-5B22-4F56-9462-C11652AA1543@gmail.com> References: <4B6CD838-689A-4829-9274-ED3456A40FA6@gmail.com> <20DF08F4-5B22-4F56-9462-C11652AA1543@gmail.com> Message-ID: <53c4db3ab59a41489447a37366a2e406@MAIL13M2N2.ad.uams.edu> Try Delaware Diamond Knives and Dorn and Hart. I believe both sell steel and I know they sell tungsten carbide. Pam Marcum -----Original Message----- From: Eileen Akemi Allison [mailto:akemiat3377 at gmail.com] Sent: Wednesday, June 10, 2015 7:25 AM To: Maria Mejia Cc: Histonet Subject: Re: [Histonet] searching for a 50cm stainless steel profile A knife Maria: Hacker Instruments sells new and reconditioned knives. They also provide a knife sharpening service for a fee of course. I believe they have that size, but if not, they will help you. Here is their link. http://www.tidylabstore.com/newmikn.html Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com Tele: (831) 375-3577 X117 > On Jun 9, 2015, at 8:28 PM, Maria Mejia wrote: > > Hello, > > Can any one tell me where I can buy a 50cm stainless steel profile A > type knife to use on a tetrander cast iron sliding microtome? Any > assistance from anyone will be greatly appreciated. > > Regards > Maria Mejia > UCSF > Mission Bay > San Francisco, CA > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Pat.Patterson at propath.com Wed Jun 10 10:51:01 2015 From: Pat.Patterson at propath.com (Pat Patterson) Date: Wed, 10 Jun 2015 15:51:01 +0000 Subject: [Histonet] Dallas IHC Position Message-ID: <6DCB8B92D0138244B56CE8EACE0D458D2E710845@Mail.propathlab.com> IMMUNOHISTOCHEMISTRY TECHNICIAN ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Immunohistochemistry Technician for its' Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. The ideal candidate will have a minimum of 4 years Histology experience with paraffin microtomy with a variety of different tissue types, prefer at least 1 - 2 years immunohistochemistry, immunofluorescence or in situ hybridization and frozen section experience. Working knowledge of IHC theory required, hands on IHC performance desired. If using an automated system we'll easily train you on our manual system. HT (ASCP) or QIHC desired. The hours for the position are 4:00 p.m. to 12:30 a.m. Monday through Friday. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EEO/AA-M/F/disability/protected veteran status Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to accessibility at propath.com. Pat Patterson, HTL(ASCP) Manager, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. From overn003 at umn.edu Wed Jun 10 10:51:53 2015 From: overn003 at umn.edu (Paula Overn) Date: Wed, 10 Jun 2015 10:51:53 -0500 Subject: [Histonet] DIF validation on Derm samples Message-ID: Hi all, I am in the process of validating DIF on derm samples and am wondering if anyone in the group can share what type of controls they used to validate this testing. Also, what is the best way to freeze your samples? Snap freeze or the routine slower method in the cryostat. Thanks in advance for your help. Paula From tejohnson at genoptix.com Wed Jun 10 12:47:18 2015 From: tejohnson at genoptix.com (Teri Johnson) Date: Wed, 10 Jun 2015 17:47:18 +0000 Subject: [Histonet] Cryojane tape-transfer and hydration of sections Message-ID: Hi Ana, The OCT on the coated slides will not disappear like it does on regular glass slides. It appears to polymerize along with the coating, but it doesn't seem to interfere on the tissue section with the staining. According to Gayle Callis' recommendation, it's best to try using the least amount of coating rather than more. More doesn't always mean better adhesion, and as Nancy Thomas reported usually ends up causing more imaging interference and stain uptake making for a messier slide. Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA 92008 USA Phone +1 760 516 5954 tejohnson at genoptix.com www.genoptix.com ________________________________ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. From gayle.callis at bresnan.net Wed Jun 10 16:18:12 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Wed, 10 Jun 2015 15:18:12 -0600 Subject: [Histonet] Cryojane bone frozen sections and immunofluorescence Message-ID: <000201d0a3c2$f5c02e60$e1408b20$@bresnan.net> Everyone wrote: Hi Ana, The OCT on the coated slides will not disappear like it does on regular glass slides. It appears to polymerize along with the coating, but it doesn't seem to interfere on the tissue section with the staining. According to Gayle Callis' recommendation, it's best to try using the least amount of coating rather than more. More doesn't always mean better adhesion, and as Nancy Thomas reported usually ends up causing more imaging interference and stain uptake making for a messier slide. Teri Johnson Manager, Clinical Trial Testing Genoptix, Inc., a Novartis company BioPharma 1811 Aston Avenue Carlsbad, CA 92008 USA Ana, We once tried 4x coated slides thinking, like you, that it would deliver a better quality section and would stay on the slide better. Our researchers did not like them at all because of the high level of background staining and fluorescence. We only use 1x now. Nancy -----Original Message----- From: Ana Maluenda [mailto: amaluenda at svi.edu.au] Subject: [Histonet] Cryojane tape-transfer and hydration of sections Hello Histonetters! I was wondering if there is anyone out there who is currently using the CryoJane Tape-transfer system for bone sections. I have been trying to get good sections for mice tibia and femur and often find myself with problems. I spent a good time trying to improve the quality of sections (tried different temperatures, thicknesses and speed as well as 1x vs 4x coated slides). The sections improved (although not perfect), but I was planning to move on for a staining trial. Currently, the sections are taken at 5um, in a 4x coated slide, at -26oC. However, now I get myself with a problem when hydrating the sections. At the moment, once the sections are taken, they are kept in -20oC (for short-storage). They are then taken out and left at RT for 30 min before hydration with PBS for another 30 min. I have noticed that it seems as if the OCT around the sections doesn't completely dissolve. I have already tried PBS vs dH2O and played around with times (from 5 min to overnight) with no differences. Has anyone had this happening before? Can it be because of the 4x coat? Is there anything I can do to? And would this be a problem for immunofluorescence? Any advice would be much appreciated! Thanks in advance, Ana Maluenda **************************************************************************** ****************************************************** To Teri and all, Thanks Teri for reiterating my suggestions along with more information. We found the 4X coating to be unacceptable. It is more sticky but the polymer is too thick and will cause more background when one needs to deal with for immunofluorescence work. With murine turbinates, unfixed and calcified, we did use the 1X and even 1/2X but it was necessary to flash the UV three times, waiting 30 sec between flashes so the capacitor could build up charge. It takes patience. We sectioned at 5 ?m but the d profile tungsten carbide knife was very sharp so do not cut on an edge you use for trimming into the block. Careful removal of the pink tape is required, inside cryostat chamber, brace the corner of slide, then pull pink tape diagonally across the section from one corner to opposite corner. You have to play with temperatures with everything the same temperature. For turbinates -30C worked well, but tape, slides, rollers, etc. blade and samples were at that temperature, including the UV platform. Sectioning temperatures vary with different laboratories, the sections can be air dried like any other frozen section destined for solvent fixation. I would go ahead and air dry a frozen section and store at -80C, not -20C since the colder temperature is more suitable for retaining antigenicity. We preferred using fresh, unfixed tissues, snap frozen correctly (not in a cryostat!) over NBF or PFA prefixed/cryoprotected snap frozen bone as we found the fresh tissue frozen section stayed transferred to the slide better. Other may have a different experience. You cannot remove the OCT from the polymerized surface and don't need to do that. There are some things your researchers will have to live with. 1) polymer exists even with 1X, but focusing on the plane of the section and what is in the section will work. 2) make sure you work with the brightest fluorophors i.e. Alexa dyes. 3) autofluorescence is caused by many things and if your sections are prefixed with NBF or PFA, then aldehyde induced auto-fluorescence will happen but can be treated. Go on web and get Autofluorescence causes and cures pdf. 4) if you work with Near infra-red fluorophores, there is no auto-fluorescence in the NIR region but the eye can't see it but the photos are spectacular. 5) Totally fill in those weird polymer gaps, be overly generous with antifade mounting media. i.e. Molecular Probes AntiFade Reagent, ready to use. 6)if working with confocal, you can rule out backgrounds/autofluorescence or with spectral imaging. We learned to ignore some of the polymer background and used only the brightest fluorophores. Alexa dyes and Dylights are two or many available now. What you want is a fluorophore signal is brighter than the goo and or autofluorescence caused by aldehyde fixation. This goo background problem is ongoing and has been a common complaint for years but if you need the undecalcified bone section, you learn to live with it. Sadly, this remains unchanged so we live with some trade offs. We have worked with 1/2X coating with success on murine bone but extra UV flashes had to be done. Turbinates are easier than tibia and femur to transfer, but it takes practice and as said before, patience. Normal hydration occurs but OCT is glued to slide along with section, and never dissolves away. If you find Cryojane to be totally unacceptable, check out this website and how they use the Kawamoto film method that results in some spectacular cryosectioning of bone along with beautiful immunofluorescent staining. Go to this site for the whole methodology outlined in how to detail. http://bonebase.org/histomorphometry This group has some excellent publications too. I hope this helps. Gayle M. Callis HTL/HT/MT(ASCP) From katherine-walters at uiowa.edu Thu Jun 11 13:47:07 2015 From: katherine-walters at uiowa.edu (Walters, Katherine S) Date: Thu, 11 Jun 2015 18:47:07 +0000 Subject: [Histonet] ammonium bromide in fixative Message-ID: <8F5174AF8E55114E8D36E4024D8B02C41A87454B@HC-MAILBOXC1-N6.healthcare.uiowa.edu> Hi all, I am studying to take the HTL certification test and ran across a reference to Formalin Ammonium Bromide. I see that it is very good for central nervous tissue fixation, it must be made fresh and that its pH is 1.5. Does anyone happen to know the reason for ammonium bromide in this fixative? I have been looking online and this has not been explained. Also, has anyone taken this test lately? I am curious as to how much old techniques, such as mercuric fixatives will be included? Thank you, Katherine S Walters Histology Director Central Microscopy Research Facility University of Iowa 76 Eckstein Medical Research Building 431 Newton Road Iowa City, Iowa 52242 319-335-8142 Facility Website: http://cmrf.research.uiowa.edu/ ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ From madeathridge at pastnashville.com Thu Jun 11 14:00:18 2015 From: madeathridge at pastnashville.com (Maryann Deathridge) Date: Thu, 11 Jun 2015 12:00:18 -0700 Subject: [Histonet] antibody spec information Message-ID: <646a166dd6c348319be1df8ec24b8899@pastnashville.com> Question: How do you handle the antibody spec sheets that come with each antibody when you order from the particular companies. We log new lot #s when we validate each antibody and each new lot. Currently we keep the spec sheet and replace the old if the lot # has changed. Since these are on-line is that CAP approved? Thanks in advance madeathridge at pastnashville.com From bcooper at chla.usc.edu Thu Jun 11 17:47:20 2015 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Thu, 11 Jun 2015 22:47:20 +0000 Subject: [Histonet] Adenovirus controls In-Reply-To: References: Message-ID: Good afternoon Histonet! I just wanted to say thank you to all who helped in our quest for Adenovirus control material. Your generosity is truly appreciated, and once again, Histonet has proven to be an amazing resource! Sincerely, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357???? bcooper at chla.usc.edu -----Original Message----- From: Cooper, Brian [mailto:bcooper at chla.usc.edu] Sent: Thursday, May 28, 2015 10:43 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Adenovirus controls Good morning Histonet, It's been about a month since I last sent out an inquiry about adenovirus control material, and I struck out badly with only one response! Does anyone have a spare adenovirus block they'd be willing to trade for something else they might need? We have a lot of control material available for special stains--Fungus, Gram +/-, AFB, etc . . . For IHC, we have Eber and TONS and TONS of Tonsil! You can contact me offline if you're more comfortable doing so. Any help would be greatly appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From jkiernan at uwo.ca Fri Jun 12 00:31:43 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Fri, 12 Jun 2015 00:31:43 -0500 Subject: [Histonet] General Genetics (lecture and lab textbooks) In-Reply-To: <7310fdde7a8a.557a6e8e@uwo.ca> References: <7360b9f56004.557673ab@uwo.ca> <7360f5506a7c.55767424@uwo.ca> <73609cf91e96.55767462@uwo.ca> <7340b2a623ee.557674a0@uwo.ca> <736085b565d5.557674e0@uwo.ca> <73b0d9171147.5576751e@uwo.ca> <73b091b42778.5576755d@uwo.ca> <73509c9f1f33.5576759b@uwo.ca> <72e0b46a13c2.557675d9@uwo.ca> <7350eac9624b.55767617@uwo.ca> <7350a68136c9.55767656@uwo.ca> <72c0fa72f9b.55767698@uwo.ca> <72c0ed8f5c98.557676d7@uwo.ca> <72d0b1ac2272.55767715@uwo.ca> <72e096361f8c.55767753@uwo.ca> <72e0c03565bb.55767792@uwo.ca> <72e0df69759c.557677d2@uwo.ca> <72e087793e7f.55767810@uwo.ca> <7300a2d218477.5579b5cd@uwo.ca> <73109c247a6d.557a6d56@uwo.ca> <73208b557798.557a6d95@uwo.ca> <73508b0565dc.557a6dd3@uwo.ca> <7350eaa91084.557a6e12@uwo.ca> <7350e7412e94.557a6e50@uwo.ca> <7310fdde7a8a.557a6e8e@uwo.ca> Message-ID: <7350dbe28c6.557a286f@uwo.ca> Dear Jorge A. Santiago-Blay, How did you learn enough about "General Genetics for science majors" to be hired to teach the subject? Do you not own at least one textbook in the field? How did you qualify to teach the subject? With the internet (Google) it will be easy to find the most recent edition of the text from which you studied General Genetics. You could buy the book or ask the publisher for a free copy. Publishers happily provide free textbooks to instructors, hoping that every student will buy a copy of the "adopted" textbook. Why ask me? My fields of interest, before I retired, were Neuroanatomy and Histochemistry, not Genetics. Does your institution have a way to warn students that an instructor does not know a suitable textbook for his course? Your students may need such a warning! J. A. Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada = = = On 08/06/15, "Jorge A. Santiago-Blay" wrote: > General Genetics (lecture and lab textbooks) > > Dear Histonet-Listers: > > I wish to know if you have recommendations of *excellent* textbooks > (lecture and labs) for General Genetics for science majors. Although I just > finished teaching an upper division Genetic Analysis course and I am well > familiarized with what is available, I am not as familiarized with la creme > de la creme for General Genetics. > > Ideally, I want a textbook that is (list is not comprehensive): > 1. As inexpensive as possible (available online, softbound, etc.). > > 2. Easy to read, understand. and profusely illustrated. > > 3. Does not assume more than intro. biology (for majors) knowledge. > > 4. Emphasizes principles of genetics, model organisms, critical thinking, > experimental design, ecological and conservation genetics, etc. > > 5. Has a generous companion packages (incl. PowerPoints, online quiz > cartridges - not the ones that reside with the publishers but the ones than > reside on campus only, etc.). For anything online, technology is as close > to 100% reliable and intuitive as possible. > > 6. Does not shy away from math and stats (because I do not shy away from > those either). > > 7. Is not divorced of the historical context of genetics. > > 8. Highlights the importance of genetics in modern life. > > 9. For labs, all of the above and not just the usual genetics exercises. I > want a book that invites exploration and engages all of us in the field. > Ideally, with a manual for s/he that may be helping with setting up the lab. > > If you have any feedback, please kindly send it directly to me, > blayjorge at gmail.com > > With gratefulness, > > Jorge > > P.S Apologies for potential duplicate emails. > > Jorge A. Santiago-Blay, PhD > blaypublishers.comblay.cfm > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From mbmphoto at gmail.com Fri Jun 12 00:35:20 2015 From: mbmphoto at gmail.com (Maria Mejia) Date: Thu, 11 Jun 2015 22:35:20 -0700 Subject: [Histonet] need manual for Tetrander sliding microtome In-Reply-To: References: Message-ID: Hello, My PI recently purchased a Tetrander sliding microtome t from Jung (made in Germany). It's the first time I'd seen such a microtome - which by the way weighs 240lb. The only thing I could on the microtome is #18768 R. Lung A6 Heidelberg. This thing is a bit of a monster of cast iron. If someone - anyone - could kindly help me find a manual for this thing I would greatly appreciate it. We are also looking for a vendor that sells a 50cm stainless steel Profile A type knife - which should fit this monster. Any help anyone can provide is very much welcomed. Best Maria Mejia Sandler Neuroscience Building San Francisco, CA From asmith at barry.edu Mon Jun 8 09:06:22 2015 From: asmith at barry.edu (Smith, Allen A) Date: Mon, 8 Jun 2015 14:06:22 +0000 Subject: [Histonet] tissue fixation-formaldehyde concentrations which is best. In-Reply-To: <7260ca6a26cb8.5572313b@uwo.ca> References: <000801d09ff8$94c245d0$be46d170$@gmail.com> <727098aa22d5b.55727498@uwo.ca> <7270e281272b7.557274d5@uwo.ca> <72809b2320fa2.55727513@uwo.ca> <73e086d92109d.55727541@uwo.ca> <7380fd2d215a8.55727622@uwo.ca> <7380cb1220cbe.55727661@uwo.ca> <73e0e56e2234a.5572769f@uwo.ca> <7280e43226b9f.557276dd@uwo.ca> <7340aeca22e6e.5572771c@uwo.ca> <7260fac225681.5572775a@uwo.ca> <7260ca6a26cb8.5572313b@uwo.ca> Message-ID: There are many books on histological technique. Most of them are expensive. The 5th edition of John Kiernan's excellent HISTOLOGICAL AND HISTOCHEMICAL METHODS will sell for $100 when it comes out in July. If that is too much for your budget, there are older books that cover 95% of what you need to know. The 4th edition of Kiernan's HISTOLOGICAL AND HISTOCHEMICAL METHODS is available used for $60, but the price may fall when the 5th edition comes out. A used 4th edition of Gretchen Humason's ANIMAL TISSUE TECHNIQUES is a great bargain at $12. Allen A. Smith Professor of Anatomy Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: John Kiernan [mailto:jkiernan at uwo.ca] Sent: Saturday, June 06, 2015 12:31 AM To: Peter Noyce; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] tissue fixation-formaldehyde concentrations which is best. Dear Peter, Some of the information you mention as "anecdotal" is wrong. Formaldehyde and paraformaldehyde are well documented in original peer-reviewed papers and in all textbooks in the fields of histotechnology and histochemistry. Your anecdote about "high concentrations of formaldehyde quickly form a 'shell' in the tissue and will stop good penetration and fixation to the deeper tissues" has no basis in published work. Paraformaldehyde is an insoluble polymer, not "non polymerized formaldehyde". There is no such thing as "4% paraformaldehyde"! It is a sad fact that many labs do not contain even one book about histotechnology. Nearly all books in the field (and there are many) have plenty of references to review articles and original literature about the techniques. There are also several websites that provide links to useful papers. Check out some of the "useful links" on the Biological Stain Commission's site: http://biologicalstaincommission.org/useful-links/ As a graduate student, you need to work from primary sources or reliable secondary sources. When you defend your thesis, you won't want to justify your fixation or staining method by saying "I got the method by asking on an internet listserver". John Kiernan Professor Emeritus Anatomy & Cell Biology University of Western Ontario London,Canada = = = On 05/06/15, Peter Noyce wrote: > Formaldehyde 37% (commonly called 100% formalin) compared to 4% ( commonly > known as 10% neutral buffered formalin)-in theory the 37% should fix quicker > and better BUT anecdotally it is said that high concentrations of > formaldehyde quickly form a "shell" in the tissue and will stop good > penetration and fixation to the deeper tissues AND over the years it has > been said anecdotally that 4% concentration is the quickest and most > complete for all sample (mammal and plant) fixation and preservation-are > these true. Please do discuss the methanol or buffers that is in the > formaldehyde, or discuss paraformaldehyde (which is non polymerized > formaldehyde with no methanol, in water). > > Regards Peter Noyce PhD student. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald at mtsac.edu Fri Jun 12 17:01:20 2015 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Fri, 12 Jun 2015 15:01:20 -0700 Subject: [Histonet] General Genetics (lecture and lab textbooks) In-Reply-To: <7350dbe28c6.557a286f@uwo.ca> References: <7360f5506a7c.55767424@uwo.ca> <73609cf91e96.55767462@uwo.ca> <7340b2a623ee.557674a0@uwo.ca> <736085b565d5.557674e0@uwo.ca> <73b0d9171147.5576751e@uwo.ca> <73b091b42778.5576755d@uwo.ca> <73509c9f1f33.5576759b@uwo.ca> <72e0b46a13c2.557675d9@uwo.ca> <7350eac9624b.55767617@uwo.ca> <7350a68136c9.55767656@uwo.ca> <72c0fa72f9b.55767698@uwo.ca> <72c0ed8f5c98.557676d7@uwo.ca> <72d0b1ac2272.55767715@uwo.ca> <72e096361f8c.55767753@uwo.ca> <72e0c03565bb.55767792@uwo.ca> <72e0df69759c.557677d2@uwo.ca> <72e087793e7f.55767810@uwo.ca> <7300a2d218477.5579b5cd@uwo.ca> <73109c247a6d.557a6d56@uwo.ca> <73208b557798.557a6d95@uwo.ca> <73508b0565dc.557a6dd3@uwo.ca> <7350eaa91084.557a6e12@uwo.ca> <7350e7412e94.55 <7350dbe28c6.557a286f@uwo.ca> Message-ID: I believe he is trying to find a textbook that others have used and cover his questions. There are many textbooks out there and it is time consuming to review all of them, so he is asking if anyone is familiar with one that covers his needs. From: John Kiernan To: "Jorge A. Santiago-Blay" Cc: "histonet at lists.utsouthwestern.edu" Date: 06/11/2015 10:33 PM Subject: Re: [Histonet] General Genetics (lecture and lab textbooks) Dear Jorge A. Santiago-Blay, How did you learn enough about "General Genetics for science majors" to be hired to teach the subject? Do you not own at least one textbook in the field? How did you qualify to teach the subject? With the internet (Google) it will be easy to find the most recent edition of the text from which you studied General Genetics. You could buy the book or ask the publisher for a free copy. Publishers happily provide free textbooks to instructors, hoping that every student will buy a copy of the "adopted" textbook. Why ask me? My fields of interest, before I retired, were Neuroanatomy and Histochemistry, not Genetics. Does your institution have a way to warn students that an instructor does not know a suitable textbook for his course? Your students may need such a warning! J. A. Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada = = = On 08/06/15, "Jorge A. Santiago-Blay" wrote: > General Genetics (lecture and lab textbooks) > > Dear Histonet-Listers: > > I wish to know if you have recommendations of *excellent* textbooks > (lecture and labs) for General Genetics for science majors. Although I just > finished teaching an upper division Genetic Analysis course and I am well > familiarized with what is available, I am not as familiarized with la creme > de la creme for General Genetics. > > Ideally, I want a textbook that is (list is not comprehensive): > 1. As inexpensive as possible (available online, softbound, etc.). > > 2. Easy to read, understand. and profusely illustrated. > > 3. Does not assume more than intro. biology (for majors) knowledge. > > 4. Emphasizes principles of genetics, model organisms, critical thinking, > experimental design, ecological and conservation genetics, etc. > > 5. Has a generous companion packages (incl. PowerPoints, online quiz > cartridges - not the ones that reside with the publishers but the ones than > reside on campus only, etc.). For anything online, technology is as close > to 100% reliable and intuitive as possible. > > 6. Does not shy away from math and stats (because I do not shy away from > those either). > > 7. Is not divorced of the historical context of genetics. > > 8. Highlights the importance of genetics in modern life. > > 9. For labs, all of the above and not just the usual genetics exercises. I > want a book that invites exploration and engages all of us in the field. > Ideally, with a manual for s/he that may be helping with setting up the lab. > > If you have any feedback, please kindly send it directly to me, > blayjorge at gmail.com > > With gratefulness, > > Jorge > > P.S Apologies for potential duplicate emails. > > Jorge A. Santiago-Blay, PhD > blaypublishers.comblay.cfm > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan at uwo.ca Fri Jun 12 23:57:35 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Fri, 12 Jun 2015 23:57:35 -0500 Subject: [Histonet] ammonium bromide in fixative In-Reply-To: <7320b1aaa68.557bb822@uwo.ca> References: <8F5174AF8E55114E8D36E4024D8B02C41A87454B@HC-MAILBOXC1-N6.healthcare.uiowa.edu> <7320f6f94684.557a745b@uwo.ca> <7380c37640a8.557a7497@uwo.ca> <7350bd3f2440.557a74d5@uwo.ca> <73509b342fb8.557a7514@uwo.ca> <7310aea57c34.557a7552@uwo.ca> <7360e1163af2.557a7608@uwo.ca> <7360a36a32dd.557a7647@uwo.ca> <7390fba96fa1.557a7685@uwo.ca> <7390986756d8.557a76c3@uwo.ca> <7390b9752164.557a7702@uwo.ca> <7390c38718b8.557a7740@uwo.ca> <73908fbc690d.557a777e@uwo.ca> <733098f57d12.557a7835@uwo.ca> <733087ae3146.557a7873@uwo.ca> <72e0f7475fd7.557a78b1@uwo.ca> <73c0c7d33967.557a78ef@uwo.ca> <73c0dac2f6f.557a792e@uwo.ca> <73c09950418d.557a796c@uwo.ca> <73c0b9cd4805.557a79aa@uwo.ca> <73c09a863c0e.557a79e3@uwo.ca> <7280d17e1c148.557ae24a@uwo.ca> <7290edb61ac17.557ae3b5@uwo.ca> <72f083e41ab08.557ae3f3@uwo.ca> <7370dc361e6b6.557ae432@uwo.ca> <73c0dacf1a113.557ae471@uwo.ca> <7280e3121b9e8.557ae4b0@uwo.ca> <73e0aafd1c6e0.557ae52c@uwo.ca> <7310d5541e775.557ae56b@uwo.ca> <72e0fb081ffb5.557ae5aa@uwo.ca> <7340bae71f39f.557ae5d1@uwo.ca> <739091565ea5.557ba99a@uwo.ca> <739093db6cc5.557ba9d8@uwo.ca> <7350e8485946.557baa53@uwo.ca> <7350bc2741f6.557baa92@uwo.ca> <7350acb343f2.557baad0@uwo.ca> <7320a0244e3d.557bab0e@uwo.ca> <7320c4d77822.557bab4c@uwo.ca> <73c0f3777c36.557bab8d@uwo.ca> <72e0930dd1b.557babcc@uwo.ca> <73c094ad716e.557bac0a@uwo.ca> <7360bf0d2def.557bac48@uwo.ca> <73608fa667c5.557bacc3@uwo.ca> <7330f6896006.557bad02@uwo.ca> <732092e86750.557bad40@uwo.ca> <72e097d36a7a.557badbb@uwo.ca> <72e0d35b2117.557badf9@uwo.ca> <7350f68736bc.557baeb0@uwo.ca> <7350cd764ed6.557baeee@uwo.ca> <73909f7cb47.557baf2d@uwo.ca> <7320c4fb595.557baf6b@uwo.ca> <72e0c1c866f.557bafa9@uwo.ca> <731087e6c1.557bafe8@uwo.ca> <7330aa137e65.557bb026@uwo.ca> <7390b83d65df.557bb064@uwo.ca> <73e090ae34a3.557bb0a5@uwo.ca> <732087fb5d7b.557bb0e4@uwo.ca> <7320d29724a7.557bb122@uwo.ca> <7360bd24459.557bb19c@uwo.ca> <7360bd5e241f.557bb1db@uwo.ca> <7380a6eb6b9a.557bb21a@uwo.ca> <7330ff463b74.557bb259@uwo.ca> <73e0cd67741.557bb297@uwo.ca> <73c0fc462132.557bb2d5@uwo.ca> <73c0ad8e1385.557bb314@uwo.ca> <7360f6252b48.557bb352@uwo.ca> <7360fcbba97.557bb391@uwo.ca> <73e0c35f66e5.557bb484@uwo.ca> <736094d158ad.557bb576@uwo.ca> <7360fb261994.557bb5b5@uwo.ca> <7320b35b65ff.557bb5f3@uwo.ca> <73e0ca012617.557bb633@uwo.ca> <7310c1bb4bc7.557bb671@uwo.ca> <7310878333ff.557bb6b1@uwo.ca> <7310a1711425.557bb7a4@uwo.ca> <736090915ed6.557bb7e3@uwo.ca> <7320b1aaa68.557bb822@uwo.ca> Message-ID: <7390ffed530f.557b71ef@uwo.ca> Formalin with ammonium bromide is the fixative prescribed for traditional silver and gold techniques for staining neuroglial cells in free-floating frozen sections. Adding ammonium bromide lowers the pH of the fixative because of a chemical reaction with formaldehyde. An Argentinian histologist called Lascano showed in the 1940s that any sufficiently acidified formaldehyde solution (pH 1.5) was just as good as FAB for Cajal's "gold-sublimate" (astrocytes) and for silver carbonate methods (oligodendrocytes, microglia). The reaction that lowers the pH is 4NH4+ + 6HCHO ---> C6H12N4 + 6H2O + 4H+ (Subscripts and superscripts in this equation will not be honoured in this email!) The 4H+ lowers the pH. The other product, C6H12N4 is hexamethylenetetramine, also known as hexamine in Britain and methenamine in the USA, and used in various histochemical staining methods, notably Grocott's method for fungal cell-walls in sections of animal (including human) tissues. The bromide (Br-) ion of NH4Br is irrelevant, in the FAB fixative and also in Globus's pretreatments (dilute ammonia followed by dilute hydrobromic acid). Globus's "bromuration" treatment was applied to frozen sections of pieces of CNS that had been fixed in non-acidified formalin. The references for Lascano's work are: Lascano, E.F. (1946a). Influencia del pH en la impregnacion argentica del tejido nervioso. Archivos de la Sociedad Argentina de Anatomia Normal y Patologica 8:105-114. Lascano, E.F. (1946b). Importancia del pH en la fijacion del tejido nervioso. Creacion artificial de fijadores tipo formol-bromuro y formol-nitrato de urano de Cajal. Archivos de la Sociedad Argentina de Anatomia Normal y Patologica 8:185-194. Lascano, E.F. (1946c). Influencia del pH del fijador en la coloracion argentica del tejido nervioso. Archivos de la Sociedad Argentina de Anatomia Normal y Patologica 8:272-276. Not everyone agreed with Lascano! Polak, M. (1948). Sobre la importancia del bromuro de amonio de la solucion fijadora de Cajal en la impregnacion argentica del tejido nervioso. Archivos de la Sociedad Argentina de Anatomia Normal y Patologica 10:224-234. Do you really need this information to pass your HTL qualifying exam? It isn't easily found in books or with Google Scholar. I came across these papers quite by chance when looking over some old journals that UWO's library had set aside for throwing out (Gasp!), about 1980. Yes, they had hit a new low! Their services have, however, been exceptionally good for the last 10-15 years. Does anyone still use either Cajal's method for astrocytes or traditional silver carbonate methods to stain oligodendrocytes and microglia? They are difficult for several reasons, take up time, and require an old-fashioned freezing microtome to cut and collect the rather thick sections that are needed. Reliable antibodies for immunostaining glial cell-types have been available for many years, and they work on any kind of section. You need some thickness to appreciate the 3D shapes of astrocytes and oligodendrocytes, however they are stained. There's plenty of histo-history in the traditional neuroglia stains, which defined the cell-types for identification by electron microscopy and immunohistochemistry. John Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada = = = On 11/06/15, "Walters, Katherine S" wrote: > Hi all, > > I am studying to take the HTL certification test and ran across a reference to Formalin Ammonium Bromide. I see that it is very good for central nervous tissue fixation, it must be made fresh and that its pH is 1.5. Does anyone happen to know the reason for ammonium bromide in this fixative? I have been looking online and this has not been explained. > > Also, has anyone taken this test lately? I am curious as to how much old techniques, such as mercuric fixatives will be included? > > Thank you, > > Katherine S Walters > Histology Director > > Central Microscopy Research Facility > University of Iowa > 76 Eckstein Medical Research Building > 431 Newton Road > Iowa City, Iowa 52242 > > 319-335-8142 > > Facility Website: > http://cmrf.research.uiowa.edu/ > > > > ________________________________ > Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. > ________________________________ > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From LBUSTAMANTE at cvm.tamu.edu Sat Jun 13 09:46:53 2015 From: LBUSTAMANTE at cvm.tamu.edu (Bustamante, Lin) Date: Sat, 13 Jun 2015 14:46:53 +0000 Subject: [Histonet] Colloidal Iron vs Alcian Blue Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC901560579F1@CVMMB02.cvm.tamu.edu> Hello, I would like to know if it was colloidal iron was more intense and also more specific than alcian blue or more intense but less specific than alcian blue? I have conflicting information regarding this stain. Thank you for your help! From chardy at csu.edu.au Sun Jun 14 05:09:40 2015 From: chardy at csu.edu.au (Hardy, Cate) Date: Sun, 14 Jun 2015 20:09:40 +1000 Subject: [Histonet] IHC on fish Message-ID: <144C8E2433CA3B4D9E080E5CDA803D05A752CC26FC@MAIL01.CSUMain.csu.edu.au> Hi all; Has anyone performed IHC on fish. I have been asked to perform this using several of our routine antibodies that I use on mammals. My gut tells me it won't work but I am a lowly histotech and don't know anything. Seeking knowledge from learned tech's with experience in such matters Thanks Cate Hardy Senior Technical Officer Veterinary Diagnostic Laboratory Charles Strut University NSW Australia Charles Sturt University | ALBURY-WODONGA | BATHURST | CANBERRA | DUBBO | GOULBURN | MELBOURNE | ONTARIO | ORANGE | PORT MACQUARIE | SYDNEY | WAGGA WAGGA | LEGAL NOTICE This email (and any attachment) is confidential and is intended for the use of the addressee(s) only. If you are not the intended recipient of this email, you must not copy, distribute, take any action in reliance on it or disclose it to anyone. Any confidentiality is not waived or lost by reason of mistaken delivery. Email should be checked for viruses and defects before opening. Charles Sturt University (CSU) does not accept liability for viruses or any consequence which arise as a result of this email transmission. Email communications with CSU may be subject to automated email filtering, which could result in the delay or deletion of a legitimate email before it is read at CSU. The views expressed in this email are not necessarily those of CSU. Charles Sturt University in Australia http://www.csu.edu.au The Grange Chancellery, Panorama Avenue, Bathurst NSW Australia 2795 (ABN: 83 878 708 551; CRICOS Provider Numbers: 00005F (NSW), 01947G (VIC), 02960B (ACT)). TEQSA Provider Number: PV12018 Charles Sturt University in Ontario http://www.charlessturt.ca 860 Harrington Court, Burlington Ontario Canada L7N 3N4 Registration: www.peqab.ca Consider the environment before printing this email. From jburch01 at gmail.com Sun Jun 14 07:24:25 2015 From: jburch01 at gmail.com (Jim Burchette) Date: Sun, 14 Jun 2015 07:24:25 -0500 Subject: [Histonet] IHC on fish In-Reply-To: <144C8E2433CA3B4D9E080E5CDA803D05A752CC26FC@MAIL01.CSUMain.csu.edu.au> References: <144C8E2433CA3B4D9E080E5CDA803D05A752CC26FC@MAIL01.CSUMain.csu.edu.au> Message-ID: Cate. Please report your findings on this topic. Kind regards. JB On Jun 14, 2015 6:38 AM, "Hardy, Cate" wrote: > Hi all; > > Has anyone performed IHC on fish. I have been asked to perform this using > several of our routine antibodies that I use on mammals. My gut tells me it > won't work but I am a lowly histotech and don't know anything. Seeking > knowledge from learned tech's with experience in such matters > > Thanks > > Cate Hardy > Senior Technical Officer > Veterinary Diagnostic Laboratory > Charles Strut University > NSW Australia > Charles Sturt University > > | ALBURY-WODONGA | BATHURST | CANBERRA | DUBBO | GOULBURN | MELBOURNE | > ONTARIO | ORANGE | PORT MACQUARIE | SYDNEY | WAGGA WAGGA | > > LEGAL NOTICE > This email (and any attachment) is confidential and is intended for the > use of the addressee(s) only. If you are not the intended recipient of this > email, you must not copy, distribute, take any action in reliance on it or > disclose it to anyone. Any confidentiality is not waived or lost by reason > of mistaken delivery. Email should be checked for viruses and defects > before opening. Charles Sturt University (CSU) does not accept liability > for viruses or any consequence which arise as a result of this email > transmission. Email communications with CSU may be subject to automated > email filtering, which could result in the delay or deletion of a > legitimate email before it is read at CSU. The views expressed in this > email are not necessarily those of CSU. > > Charles Sturt University in Australia http://www.csu.edu.au The Grange > Chancellery, Panorama Avenue, Bathurst NSW Australia 2795 (ABN: 83 878 708 > 551; CRICOS Provider Numbers: 00005F (NSW), 01947G (VIC), 02960B (ACT)). > TEQSA Provider Number: PV12018 > > Charles Sturt University in Ontario http://www.charlessturt.ca 860 > Harrington Court, Burlington Ontario Canada L7N 3N4 Registration: > www.peqab.ca > > Consider the environment before printing this email. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From owensc500 at gmail.com Mon Jun 15 07:43:31 2015 From: owensc500 at gmail.com (Clarence Owens) Date: Mon, 15 Jun 2015 08:43:31 -0400 Subject: [Histonet] Paraffin Blocks Message-ID: <693DCCEC-DACA-4FCD-9E78-E73453855C66@gmail.com> Hello Histoneters, Maybe one of you could assist me in search of some cancer blocks. I am in search for Ewing's sarcoma, rhabdomyosarcoma, Mesothelioma, and Small Cell Sarcoma. I am looking for true patient disease tissue not a clone. If anyone has any additional block or fixed tissue they could spare it would be very much appreciated. We will purchase these blocks from you or your institution. Please contact me if you can assist me. Thank you. Regards, Clarence Owens, HT (ASCP) QIHC Sent from my iPhone From TGoins at mt.gov Mon Jun 15 09:01:22 2015 From: TGoins at mt.gov (Goins, Tresa) Date: Mon, 15 Jun 2015 14:01:22 +0000 Subject: [Histonet] IHC on fish In-Reply-To: <144C8E2433CA3B4D9E080E5CDA803D05A752CC26FC@MAIL01.CSUMain.csu.edu.au> References: <144C8E2433CA3B4D9E080E5CDA803D05A752CC26FC@MAIL01.CSUMain.csu.edu.au> Message-ID: Cate - Your gut is probably right but results are unpredictable. I have forwarded your query to the Bozeman Fish Technology center to see if they can offer any assistance. Tresa -----Original Message----- From: Hardy, Cate [mailto:chardy at csu.edu.au] Sent: Sunday, June 14, 2015 4:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC on fish Hi all; Has anyone performed IHC on fish. I have been asked to perform this using several of our routine antibodies that I use on mammals. My gut tells me it won't work but I am a lowly histotech and don't know anything. Seeking knowledge from learned tech's with experience in such matters Thanks Cate Hardy Senior Technical Officer Veterinary Diagnostic Laboratory Charles Strut University NSW Australia Charles Sturt University | ALBURY-WODONGA | BATHURST | CANBERRA | DUBBO | GOULBURN | MELBOURNE | ONTARIO | ORANGE | PORT MACQUARIE | SYDNEY | WAGGA WAGGA | LEGAL NOTICE This email (and any attachment) is confidential and is intended for the use of the addressee(s) only. If you are not the intended recipient of this email, you must not copy, distribute, take any action in reliance on it or disclose it to anyone. Any confidentiality is not waived or lost by reason of mistaken delivery. Email should be checked for viruses and defects before opening. Charles Sturt University (CSU) does not accept liability for viruses or any consequence which arise as a result of this email transmission. Email communications with CSU may be subject to automated email filtering, which could result in the delay or deletion of a legitimate email before it is read at CSU. The views expressed in this email are not necessarily those of CSU. Charles Sturt University in Australia http://www.csu.edu.au The Grange Chancellery, Panorama Avenue, Bathurst NSW Australia 2795 (ABN: 83 878 708 551; CRICOS Provider Numbers: 00005F (NSW), 01947G (VIC), 02960B (ACT)). TEQSA Provider Number: PV12018 Charles Sturt University in Ontario http://www.charlessturt.ca 860 Harrington Court, Burlington Ontario Canada L7N 3N4 Registration: www.peqab.ca Consider the environment before printing this email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmilne at bccrc.ca Mon Jun 15 10:23:22 2015 From: kmilne at bccrc.ca (Katy Milne) Date: Mon, 15 Jun 2015 15:23:22 +0000 Subject: [Histonet] IHC on fish In-Reply-To: References: Message-ID: I stained some zebra fish many many years ago. For the life of me, I can't remember with what! It's all going to come down to whether or not your Abs can recognize it. I'd say give it a go and don't trust a negative result or look for Abs that should specifically work for fish. Not all companies even test that obviously but there are a few that do. I used to work for a company that would test Abs for western blots against pretty much anything that would go in a blender! :) What antigens are you looking for? Good luck. Katy Message: 1 Date: Sun, 14 Jun 2015 20:09:40 +1000 From: "Hardy, Cate" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] IHC on fish Message-ID: <144C8E2433CA3B4D9E080E5CDA803D05A752CC26FC at MAIL01.CSUMain.csu.edu.au> Content-Type: text/plain; charset="us-ascii" Hi all; Has anyone performed IHC on fish. I have been asked to perform this using several of our routine antibodies that I use on mammals. My gut tells me it won't work but I am a lowly histotech and don't know anything. Seeking knowledge from learned tech's with experience in such matters Thanks Cate Hardy Senior Technical Officer Veterinary Diagnostic Laboratory Charles Strut University NSW Australia Charles Sturt University | ALBURY-WODONGA | BATHURST | CANBERRA | DUBBO | GOULBURN | MELBOURNE | | ONTARIO | ORANGE | PORT MACQUARIE | SYDNEY | WAGGA WAGGA | From tgenade at gmail.com Mon Jun 15 11:02:51 2015 From: tgenade at gmail.com (Tyrone Genade) Date: Mon, 15 Jun 2015 11:02:51 -0500 Subject: [Histonet] IHC on fish Message-ID: Hello, > From: "Hardy, Cate" > Subject: [Histonet] IHC on fish > > Hi all; > > Has anyone performed IHC on fish. I have been asked to perform this using > several of our routine antibodies that I use on mammals. My gut tells me it > won't work but I am a lowly histotech and don't know anything. Seeking > knowledge from learned tech's with experience in such matters > > Thanks > My entire thesis was about taking antibodies generated against mammalian antigens and using them to stain equivalent structures in fish. In many cases the results were as expected. I didn't modify the protocols in any way. Simply use the same protocol you are using for the mammals and apply them to the fish. What did save me a lot of time was doing western blots first to test for immunoreactivity and specificity. Some of my pretty micrographs had to be discarded because on a western blot the antibody labeled multiple bands irregardless of the antibody dilution. If you know the immunogen sequences blast the various fish protein sequence databases and see how well the antigen is conserved in various species. If there is low similarity in the sequence then it isn't worth testing the antibody. Again, confirm with a western blot or dot-blot for immunoreactivity. As regards fish sectioning the little bones can be a bother, and the tissues seems to dry out and harden very fast in ethanol. I would suggest something like Davidson's fixative, overnight at 4 oC and then quickly process the tissue. For antigen retrieval you can modify the protocol of http://www.ncbi.nlm.nih.gov/pubmed/21603650 for sections. You can also try Citrate Buffer Antigen retrieval. If you are going to do immunofluorescence then the protocol I got from Gayle Callis works well: after taking water incubate slides or section twice in 300 mM glycine in TBS, pH 7.4, 15 minutes at room temperature (RT) to quench autofluorescence. Good luck with the antibodies! -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From katherine-walters at uiowa.edu Mon Jun 15 11:32:46 2015 From: katherine-walters at uiowa.edu (Walters, Katherine S) Date: Mon, 15 Jun 2015 16:32:46 +0000 Subject: [Histonet] ammonium bromide in fixative In-Reply-To: <7390ffed530f.557b71ef@uwo.ca> References: <7320b1aaa68.557bb822@uwo.ca> <7390ffed530f.557b71ef@uwo.ca> Message-ID: <8F5174AF8E55114E8D36E4024D8B02C41A87493C@HC-MAILBOXC1-N6.healthcare.uiowa.edu> Thank you for such a detailed response. It is doubtful I would have found this information without you. The National Histology Society online learning center is one of my study resources, this is where the question came from. They also include several mercuric fixatives that we would never use in our lab in this day and age. Perhaps the material has not been updated for a number of years. I have not yet heard from any recent exam takers about the content of old techniques. I find them interesting, but don't know how much time to devote to them. Katherine S Walters Histology Director Central Microscopy Research Facility University of Iowa 76 Eckstein Medical Research Building 431 Newton Road Iowa City, Iowa 52242 319-335-8142 Facility Website: http://cmrf.research.uiowa.edu/ From: John Kiernan [mailto:jkiernan at uwo.ca] Sent: Friday, June 12, 2015 11:58 PM To: Walters, Katherine S; Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] ammonium bromide in fixative Formalin with ammonium bromide is the fixative prescribed for traditional silver and gold techniques for staining neuroglial cells in free-floating frozen sections. Adding ammonium bromide lowers the pH of the fixative because of a chemical reaction with formaldehyde. An Argentinian histologist called Lascano showed in the 1940s that any sufficiently acidified formaldehyde solution (pH 1.5) was just as good as FAB for Cajal's "gold-sublimate" (astrocytes) and for silver carbonate methods (oligodendrocytes, microglia). The reaction that lowers the pH is 4NH4+ + 6HCHO ---> C6H12N4 + 6H2O + 4H+ (Subscripts and superscripts in this equation will not be honoured in this email!) The 4H+ lowers the pH. The other product, C6H12N4 is hexamethylenetetramine, also known as hexamine in Britain and methenamine in the USA, and used in various histochemical staining methods, notably Grocott's method for fungal cell-walls in sections of animal (including human) tissues. The bromide (Br-) ion of NH4Br is irrelevant, in the FAB fixative and also in Globus's pretreatments (dilute ammonia followed by dilute hydrobromic acid). Globus's "bromuration" treatment was applied to frozen sections of pieces of CNS that had been fixed in non-acidified formalin. The references for Lascano's work are: Lascano, E.F. (1946a). Influencia del pH en la impregnacion argentica del tejido nervioso. Archivos de la Sociedad Argentina de Anatomia Normal y Patologica 8:105-114. Lascano, E.F. (1946b). Importancia del pH en la fijacion del tejido nervioso. Creacion artificial de fijadores tipo formol-bromuro y formol-nitrato de urano de Cajal. Archivos de la Sociedad Argentina de Anatomia Normal y Patologica 8:185-194. Lascano, E.F. (1946c). Influencia del pH del fijador en la coloracion argentica del tejido nervioso. Archivos de la Sociedad Argentina de Anatomia Normal y Patologica 8:272-276. Not everyone agreed with Lascano! Polak, M. (1948). Sobre la importancia del bromuro de amonio de la solucion fijadora de Cajal en la impregnacion argentica del tejido nervioso. Archivos de la Sociedad Argentina de Anatomia Normal y Patologica 10:224-234. Do you really need this information to pass your HTL qualifying exam? It isn't easily found in books or with Google Scholar. I came across these papers quite by chance when looking over some old journals that UWO's library had set aside for throwing out (Gasp!), about 1980. Yes, they had hit a new low! Their services have, however, been exceptionally good for the last 10-15 years. Does anyone still use either Cajal's method for astrocytes or traditional silver carbonate methods to stain oligodendrocytes and microglia? They are difficult for several reasons, take up time, and require an old-fashioned freezing microtome to cut and collect the rather thick sections that are needed. Reliable antibodies for immunostaining glial cell-types have been available for many years, and they work on any kind of section. You need some thickness to appreciate the 3D shapes of astrocytes and oligodendrocytes, however they are stained. There's plenty of histo-history in the traditional neuroglia stains, which defined the cell-types for identification by electron microscopy and immunohistochemistry. John Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada = = = On 11/06/15, "Walters, Katherine S" > wrote: Hi all, I am studying to take the HTL certification test and ran across a reference to Formalin Ammonium Bromide. I see that it is very good for central nervous tissue fixation, it must be made fresh and that its pH is 1.5. Does anyone happen to know the reason for ammonium bromide in this fixative? I have been looking online and this has not been explained. Also, has anyone taken this test lately? I am curious as to how much old techniques, such as mercuric fixatives will be included? Thank you, Katherine S Walters Histology Director Central Microscopy Research Facility University of Iowa 76 Eckstein Medical Research Building 431 Newton Road Iowa City, Iowa 52242 319-335-8142 Facility Website: http://cmrf.research.uiowa.edu/ ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you. ________________________________ From thoward at unm.edu Mon Jun 15 11:59:47 2015 From: thoward at unm.edu (Tamara Howard) Date: Mon, 15 Jun 2015 16:59:47 +0000 Subject: [Histonet] IHC on fish In-Reply-To: References: Message-ID: Cate - Why not do a Blast search for the sequences your anti-mammal antibodies recognize & see if they occur in fish? Some proteins are strongly conserved, or have conserved domains - you might get lucky! I tried a new (to me) antibody last week that was made against an amoeba, and it worked gangbusters on mouse and rat. Tamara ................................................... Tamara Howard Dept. of Cell Biology & Physiology University of New Mexico Albuquerque, NM Message: 1 Date: Sun, 14 Jun 2015 20:09:40 +1000 From: "Hardy, Cate" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] IHC on fish Message-ID: <144C8E2433CA3B4D9E080E5CDA803D05A752CC26FC at MAIL01.CSUMain.csu.edu.au> Content-Type: text/plain; charset="us-ascii" Hi all; Has anyone performed IHC on fish. I have been asked to perform this using several of our routine antibodies that I use on mammals. My gut tells me it won't work but I am a lowly histotech and don't know anything. Seeking knowledge from learned tech's with experience in such matters Thanks Cate Hardy Senior Technical Officer Veterinary Diagnostic Laboratory Charles Strut University NSW Australia Charles Sturt University | ALBURY-WODONGA | BATHURST | CANBERRA | DUBBO | GOULBURN | MELBOURNE | ONTARIO | ORANGE | PORT MACQUARIE | SYDNEY | WAGGA WAGGA | LEGAL NOTICE This email (and any attachment) is confidential and is intended for the use of the addressee(s) only. If you are not the intended recipient of this email, you must not copy, distribute, take any action in reliance on it or disclose it to anyone. Any confidentiality is not waived or lost by reason of mistaken delivery. Email should be checked for viruses and defects before opening. Charles Sturt University (CSU) does not accept liability for viruses or any consequence which arise as a result of this email transmission. Email communications with CSU may be subject to automated email filtering, which could result in the delay or deletion of a legitimate email before it is read at CSU. The views expressed in this email are not necessarily those of CSU. Charles Sturt University in Australia http://www.csu.edu.au The Grange Chancellery, Panorama Avenue, Bathurst NSW Australia 2795 (ABN: 83 878 708 551; CRICOS Provider Numbers: 00005F (NSW), 01947G (VIC), 02960B (ACT)). TEQSA Provider Number: PV12018 Charles Sturt University in Ontario http://www.charlessturt.ca 860 Harrington Court, Burlington Ontario Canada L7N 3N4 Registration: www.peqab.ca Consider the environment before printing this email. ------------------------------ From Mary.Leslie at tricore.org Mon Jun 15 15:18:59 2015 From: Mary.Leslie at tricore.org (Leslie, Mary) Date: Mon, 15 Jun 2015 20:18:59 +0000 Subject: [Histonet] Processing Validation Message-ID: Does anyone have a processing validation procedure they would like to share? Thank you! Mary Leslie From KSimeone at leavittmgt.com Mon Jun 15 15:26:24 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Mon, 15 Jun 2015 20:26:24 +0000 Subject: [Histonet] FT position HISTOTECHNOLOGIST/IHC Delray Beach, FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C3DC9AEF8@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Dermatology Lab. This is a permanent full time SECOND SHIFT (40 hours) position with benefits (medical/401k/vacation) and competitive pay. THIS IS A DRUG FREE WORKPLACE. Drug testing, background check and personality testing is required. ONLY SERIOUS INQURIES, please read EVERY qualification desired. Sorry, NO relocation assistance offered. Position available for training immediately. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengimann at leavittmgt.com if interested. *Full time position Mon-Fri 2p-10:30p (IMMUNOHISTOCHEMISTRY/SPECIALS department) *MUST be licensed as a FLORIDA HISTOTECHNOLOGIST (this is NOT negotiable) *EXPERIENCE WITH IHC A MUST! Leica (BOND) and Roche/Ventana equipment experience preferred *Must be able to multi-task special stains *MUST have at LEAST 2 years experience. Please DO NOT respond if you do not possess EXPERIENCE in this area! *must be confidant, quick learner, self motivated, reliable and a team player Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From mjones at metropath.com Mon Jun 15 16:05:38 2015 From: mjones at metropath.com (Michael Ann Jones) Date: Mon, 15 Jun 2015 21:05:38 +0000 Subject: [Histonet] Processing Validation Message-ID: Would love to see also. Michael Ann On 6/15/15, 2:18 PM, "Leslie, Mary" wrote: >Does anyone have a processing validation procedure they would like to >share? Thank you! > >Mary Leslie >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcolbert at pathmdlabs.com Tue Jun 16 11:25:28 2015 From: lcolbert at pathmdlabs.com (Laurie Colbert) Date: Tue, 16 Jun 2015 16:25:28 +0000 Subject: [Histonet] Histotech positions in Los Angeles Message-ID: <12ECD7346266D74691EC2BFC75285E456DA1D58E@BFL323E10.pathmdlabs.local> PATH MD, a state-of-the-art pathology lab in West Hollywood, has two graveyard positions open - one full time and one per diem. ASCP required. Full time - Sunday night through Thursday night, 10:00 pm - 6:30 am. ASCP and two years histology (embedding, cutting, manual special stains) experience required; full benefits - medical, dental, vision, 401(k) plan with employer matching. Per diem - Sunday nights through Thursday nights, as needed. Hours worked would vary (4-8 hours) but starting time is 10:00 pm. ASCP and at least 6 months of histology (embedding, cutting, manual stains) required, but the main duty would be embedding. Both positions are available immediately. We are a growing lab and have a great "team" environment. Please forward all resumes to this email. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From srishan at mail.holyname.org Tue Jun 16 13:16:30 2015 From: srishan at mail.holyname.org (srishan at mail.holyname.org) Date: Tue, 16 Jun 2015 14:16:30 -0400 Subject: [Histonet] (no subject) Message-ID: Hi All, Does anyone know where we could get the original grey antibody storage racks used to store antibodies in the refrigerator? We are using ventana antibodies. Thanks in advance. Mala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From vanessa.keeton03 at gmail.com Tue Jun 16 14:40:32 2015 From: vanessa.keeton03 at gmail.com (Vanessa Keeton) Date: Tue, 16 Jun 2015 15:40:32 -0400 Subject: [Histonet] Myogenin Message-ID: I am looking for information on Myogenin IHC stain such as origin/history, uses, and any other pertinent information for a research paper for college. Thank you in advance. From a_schade at conradweiser.org Tue Jun 16 15:33:19 2015 From: a_schade at conradweiser.org (Schade, Adelle) Date: Tue, 16 Jun 2015 20:33:19 +0000 Subject: [Histonet] high school donations Message-ID: Hello, I am posting to ask for any information or direction this group may be able to give (or leads for contacts) concerning the possibility of donations to a high school science/ histology laboratory. I am a high school teacher (Conrad Weiser HS, Robesonia, PA) and Cell/ Molecular Biology PhD student (Univ. of the Sciences, Philadelphia, PA). I developed a histology laboratory this year in my high school and it has taken off! The students are extremely interested in learning and experimenting utilizing histological technique. I am holding a summer program opening the lab for students to work. Initially I thought I would have 5 to 10 students that would want to work over the summer; 28 students are attending. It is inspiring to see such enthusiasm! With this in mind, I would like to try to accumulate some additional equipment/ supplies. If you know of any laboratories that are closing/ shutting down/ or equipment/supplies that are not being utilized and there is a possibility of donating these items to a public high school please email me off list (a_schade at conradweiser.org). We are looking for any histology related equipment and supplies (especially a tissue processor- we are manually processing at this point); as well as any basic laboratory equipment/ supplies (glassware, micropipettes, heating mantles, etc.) Thank you for your time. I love reading the informative posts on this listserv! Have a great summer, Adelle Schade Ms. Adelle L. Schade, B.S., M.Ed., M.S. Conrad Weiser High School Anatomy and Physiology Teacher 44 Big Spring Rd. Robesonia, PA 19551 a_schade at conradweiser.org 610-693-8599 ext. 6736 From bakevictoria at gmail.com Tue Jun 16 16:59:11 2015 From: bakevictoria at gmail.com (Victoria Baker) Date: Tue, 16 Jun 2015 17:59:11 -0400 Subject: [Histonet] Decontamination of Ventana Ultra Message-ID: Hi, Recently we were told that we could no longer use our Bio-med department for doing decontamination of our instruments and that the techs would be given an in-service to learn how to do them. I remember this process as long and messy. The Ventana rep has explained to the staff as being approx. 5 hours and not difficult to do. I remember it differently so I'd like to see what other Ventana users are doing with this process. Here are my questions: Are you currently using in house Bio-engineering to do your quarterly cleanings? Do you pay Ventana to come in and do it? Are you having technical staff do it on off hours? Then give them time off another day? How extensive of a decontamination do you do? (ex - cleaning filters, extra DI rinses, pH testing of containers to be sure all cleaning reagents have been removed, forced air drying of bulk containers - etc) Have you had problems with air pressure error codes or do you increase the pressure to handle the increased fluid flow? Have you ever had overflow problems that damaged the instrument? How many instruments do you have? These are the basic questions, but if anyone has any further information or ideas - I would appreciate hearing them. Thanks Vikki From travelhistotech at hotmail.com Tue Jun 16 23:16:28 2015 From: travelhistotech at hotmail.com (Travel Histotech HTL) Date: Tue, 16 Jun 2015 21:16:28 -0700 Subject: [Histonet] Fairbanks, Alaska job opening Message-ID: Hello everyone and especially adventurous job seekers, I?m a travel histotech currently contracting at Fairbanks Memorial Hospital in sunny Fairbanks, Alaska where they have a full time opening for either a licensed or unlicensed histotech. It has not been advertised yet, so if you (or anyone you know) are fun and energetic and especially adventurous and would like to experience something different, contact the Laboratory Department Manager: Roger Herron at Roger.Herron at bannerhealth.com Or, feel free to contact me to find out more about the job first hand at travelhistotech at hotmail.com. Fairbanks is definitely an unforgettable adventure. Check it out: http://www.explorefairbanks.com/ Cheers! Jonathan Ward Edmondson, HTL From ryeo at wchosp.org Wed Jun 17 05:36:21 2015 From: ryeo at wchosp.org (Richard Yeo) Date: Wed, 17 Jun 2015 10:36:21 +0000 Subject: [Histonet] Bond Max Available Message-ID: Histonetters, I have a four year old Leica Bond Max just out of cocntract. Used very little, and is in good shape. We are going to sell it. If any one is interested please contact me. It has had its yearly PM's and has all the accessories with it. Thank you Rich Y Histology Supervisor Wooster Community Hospital 1761 Beall Ave. Wooster Ohio 44691 330-263 8563 From TanyaAbbott at catholichealth.net Wed Jun 17 13:00:50 2015 From: TanyaAbbott at catholichealth.net (Abbott, Tanya) Date: Wed, 17 Jun 2015 18:00:50 +0000 Subject: [Histonet] Subject: high school donations Message-ID: <852F7D2C14FB464D80E182B15DB138AF6B7BFF1F@CHIEX005.CHI.catholichealth.net> HI all, I've had the pleasure of meeting Adelle and two of our students! She has a wonderful program; her students are knowledgeable and very enthusiastic. Please help her out wherever you can! Tanya Abbott St Joseph Medical Center Reading PA -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Wednesday, June 17, 2015 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 139, Issue 17 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=Pq8lGo9vBgVTPM4zyPM7eBABVVQ6LIhqHAGCtKc2IZI&s=MmwqfZaOO3NzpJceGkVFp4PUfR_C_r_HxQ8KogSop88&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. (no subject) (srishan at mail.holyname.org) 2. Myogenin (Vanessa Keeton) 3. high school donations (Schade, Adelle) 4. Decontamination of Ventana Ultra (Victoria Baker) 5. Fairbanks, Alaska job opening (Travel Histotech HTL) 6. Bond Max Available (Richard Yeo) ---------------------------------------------------------------------- Message: 1 Date: Tue, 16 Jun 2015 14:16:30 -0400 From: srishan at mail.holyname.org To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] (no subject) Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi All, Does anyone know where we could get the original grey antibody storage racks used to store antibodies in the refrigerator? We are using ventana antibodies. Thanks in advance. Mala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ------------------------------ Message: 2 Date: Tue, 16 Jun 2015 15:40:32 -0400 From: Vanessa Keeton To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Myogenin Message-ID: Content-Type: text/plain; charset=UTF-8 I am looking for information on Myogenin IHC stain such as origin/history, uses, and any other pertinent information for a research paper for college. Thank you in advance. ------------------------------ Message: 3 Date: Tue, 16 Jun 2015 20:33:19 +0000 From: "Schade, Adelle" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] high school donations Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello, I am posting to ask for any information or direction this group may be able to give (or leads for contacts) concerning the possibility of donations to a high school science/ histology laboratory. I am a high school teacher (Conrad Weiser HS, Robesonia, PA) and Cell/ Molecular Biology PhD student (Univ. of the Sciences, Philadelphia, PA). I developed a histology laboratory this year in my high school and it has taken off! The students are extremely interested in learning and experimenting utilizing histological technique. I am holding a summer program opening the lab for students to work. Initially I thought I would have 5 to 10 students that would want to work over the summer; 28 students are attending. It is inspiring to see such enthusiasm! With this in mind, I would like to try to accumulate some additional equipment/ supplies. If you know of any laboratories that are closing/ shutting down/ or equipment/supplies that are not being utilized and there is a possibility of donating these items to a public high school please email me off list (a_schade at conradweiser.org). We are looking for any histology related equipment and supplies (especially a tissue processor- we are manually processing at this point); as well as any basic laboratory equipment/ supplies (glassware, micropipettes, heating mantles, etc.) Thank you for your time. I love reading the informative posts on this listserv! Have a great summer, Adelle Schade Ms. Adelle L. Schade, B.S., M.Ed., M.S. Conrad Weiser High School Anatomy and Physiology Teacher 44 Big Spring Rd. Robesonia, PA 19551 a_schade at conradweiser.org 610-693-8599 ext. 6736 ------------------------------ Message: 4 Date: Tue, 16 Jun 2015 17:59:11 -0400 From: Victoria Baker To: Histo Net list server , histonet Subject: [Histonet] Decontamination of Ventana Ultra Message-ID: Content-Type: text/plain; charset=UTF-8 Hi, Recently we were told that we could no longer use our Bio-med department for doing decontamination of our instruments and that the techs would be given an in-service to learn how to do them. I remember this process as long and messy. The Ventana rep has explained to the staff as being approx. 5 hours and not difficult to do. I remember it differently so I'd like to see what other Ventana users are doing with this process. Here are my questions: Are you currently using in house Bio-engineering to do your quarterly cleanings? Do you pay Ventana to come in and do it? Are you having technical staff do it on off hours? Then give them time off another day? How extensive of a decontamination do you do? (ex - cleaning filters, extra DI rinses, pH testing of containers to be sure all cleaning reagents have been removed, forced air drying of bulk containers - etc) Have you had problems with air pressure error codes or do you increase the pressure to handle the increased fluid flow? Have you ever had overflow problems that damaged the instrument? How many instruments do you have? These are the basic questions, but if anyone has any further information or ideas - I would appreciate hearing them. Thanks Vikki ------------------------------ Message: 5 Date: Tue, 16 Jun 2015 21:16:28 -0700 From: Travel Histotech HTL To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Fairbanks, Alaska job opening Message-ID: Content-Type: text/plain; charset="Windows-1252" Hello everyone and especially adventurous job seekers, I?m a travel histotech currently contracting at Fairbanks Memorial Hospital in sunny Fairbanks, Alaska where they have a full time opening for either a licensed or unlicensed histotech. It has not been advertised yet, so if you (or anyone you know) are fun and energetic and especially adventurous and would like to experience something different, contact the Laboratory Department Manager: Roger Herron at Roger.Herron at bannerhealth.com Or, feel free to contact me to find out more about the job first hand at travelhistotech at hotmail.com. Fairbanks is definitely an unforgettable adventure. Check it out: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.explorefairbanks.com_&d=AwICAg&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=Pq8lGo9vBgVTPM4zyPM7eBABVVQ6LIhqHAGCtKc2IZI&s=9pI-m4q0fB6ba_Enc48LpBBF8WVw0k1JPv7hc9nocc0&e= Cheers! Jonathan Ward Edmondson, HTL ------------------------------ Message: 6 Date: Wed, 17 Jun 2015 10:36:21 +0000 From: Richard Yeo To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Bond Max Available Message-ID: Content-Type: text/plain; charset="iso-8859-1" Histonetters, I have a four year old Leica Bond Max just out of cocntract. Used very little, and is in good shape. We are going to sell it. If any one is interested please contact me. It has had its yearly PM's and has all the accessories with it. Thank you Rich Y Histology Supervisor Wooster Community Hospital 1761 Beall Ave. Wooster Ohio 44691 330-263 8563 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=ND-Z_FnoJTOCBd6ZraMT0-wJD0GDS_U0VV_Zq7yxAI4&r=hZcGVKviJYVs6fzc5J4jSeysau9AxOWpKXzfQgGZmaw&m=Pq8lGo9vBgVTPM4zyPM7eBABVVQ6LIhqHAGCtKc2IZI&s=MmwqfZaOO3NzpJceGkVFp4PUfR_C_r_HxQ8KogSop88&e= ------------------------------ End of Histonet Digest, Vol 139, Issue 17 ***************************************** This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From litepath2000 at yahoo.com Wed Jun 17 15:22:18 2015 From: litepath2000 at yahoo.com (NYSHisto) Date: Wed, 17 Jun 2015 20:22:18 +0000 (UTC) Subject: [Histonet] Coverglass Message-ID: <1309865673.823297.1434572538269.JavaMail.yahoo@mail.yahoo.com> Does anyone know a source/company that provides? 22x75 coverglass?Thanks in advance From pdefazio802 at gmail.com Thu Jun 18 06:47:49 2015 From: pdefazio802 at gmail.com (Pam DeFazio) Date: Thu, 18 Jun 2015 07:47:49 -0400 Subject: [Histonet] Leica cerebo Message-ID: Anyone using this? We use cerner copath. Thoughts? From lcolbert at pathmdlabs.com Thu Jun 18 10:41:18 2015 From: lcolbert at pathmdlabs.com (Laurie Colbert) Date: Thu, 18 Jun 2015 15:41:18 +0000 Subject: [Histonet] Thermo Excelsior Processor Message-ID: <12ECD7346266D74691EC2BFC75285E456DA1FBEB@BFL323E10.pathmdlabs.local> Is there anyone who has a Thermo Excelsior AS processor and is running only biopsies on it? If so, are you having any issues with the alcohols not diluting down enough as the processor dumps and rotates the reagents due to the low amount of water in the biopsies? Feel free to contact me directly to discuss this. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From jill.cox at cox.net Thu Jun 18 11:00:43 2015 From: jill.cox at cox.net (Jill Cox) Date: Thu, 18 Jun 2015 09:00:43 -0700 Subject: [Histonet] LAB furniture Message-ID: <61FC2208-07DB-44B8-98BF-64458EC4D060@cox.net> Hi, I am in the process of adding lab furniture to the lab and was wondering if anyone has a good company that will make workbenches. I am adding an island trying to get 4' x 12'. Someone local to the Phoenix area would be great. Thank you in advance! Sent from my iPhone From jaylundgren at gmail.com Thu Jun 18 11:44:12 2015 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 18 Jun 2015 11:44:12 -0500 Subject: [Histonet] LAB furniture In-Reply-To: <61FC2208-07DB-44B8-98BF-64458EC4D060@cox.net> References: <61FC2208-07DB-44B8-98BF-64458EC4D060@cox.net> Message-ID: Try a trim carpenter shop in your area, look up custom kitchen cabinets. On Thu, Jun 18, 2015 at 11:00 AM, Jill Cox wrote: > Hi, > I am in the process of adding lab furniture to the lab and was wondering > if anyone has a good company that will make workbenches. I am adding an > island trying to get 4' x 12'. Someone local to the Phoenix area would be > great. Thank you in advance! > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Dawn.Olszewski at SGMC.ORG Fri Jun 19 09:52:50 2015 From: Dawn.Olszewski at SGMC.ORG (Olszewski, Dawn) Date: Fri, 19 Jun 2015 10:52:50 -0400 Subject: [Histonet] IHC turnaround time Message-ID: <1ED27760CE36ED4CA7F8B0F090E2AE0D314E2237@SG-MAIL-01.sgmc.org> Hi Histonetters, 6-19-15 We are wondering what the average turnaround times for IHC are for other labs. We use the Biocare Intellipath instrumentation. It holds up to 50 slides ( 10 slides per 5 racks) with continuous feed per open rack. All IHC orders placed by 12pm are usually out the same day by 5pm. We average 434 IHC slides per month and have a staff of 3 FTE's. A pathologist has voiced concerns over our IHC output. We are trying to determine "best practices" for IHC turnaround time as measured from time order placed to time of slide delivery. If you could respond with your IHC TAT including number of techs and average of IHC slides monthly, we would appreciate any input you may be able to provide. Thank you in advance, Dawn Olszewski, HTL(ASCP)QIHC From Richard.Cartun at hhchealth.org Fri Jun 19 10:20:37 2015 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 19 Jun 2015 15:20:37 +0000 Subject: [Histonet] IHC turnaround time Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E3EE9E685@HHCEXCHMB03.hhcsystem.org> I direct a busy and very efficient (I think) IHC lab staffed with 2.875 FTEs who spend most of their time doing IHC, but they also help Histology embed and cut paraffin blocks when needed (which is most days). For 2015 we are averaging 4,022 IHC patient test slides and 1,650 IHC control slides per month. Our IHC lab is staffed from 4:30 AM to 6 PM (or later depending on workload). We have 5 Leica Bond Max platforms; however, we still do some IHCs on the bench. We usually do 2-3 runs per day and an overnight run (except on Fridays) as well. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Olszewski, Dawn [mailto:Dawn.Olszewski at SGMC.ORG] Sent: Friday, June 19, 2015 10:53 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC turnaround time Hi Histonetters, 6-19-15 We are wondering what the average turnaround times for IHC are for other labs. We use the Biocare Intellipath instrumentation. It holds up to 50 slides ( 10 slides per 5 racks) with continuous feed per open rack. All IHC orders placed by 12pm are usually out the same day by 5pm. We average 434 IHC slides per month and have a staff of 3 FTE's. A pathologist has voiced concerns over our IHC output. We are trying to determine "best practices" for IHC turnaround time as measured from time order placed to time of slide delivery. If you could respond with your IHC TAT including number of techs and average of IHC slides monthly, we would appreciate any input you may be able to provide. Thank you in advance, Dawn Olszewski, HTL(ASCP)QIHC _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From rjbuesa at yahoo.com Fri Jun 19 10:24:09 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 19 Jun 2015 15:24:09 +0000 (UTC) Subject: [Histonet] IHC turnaround time In-Reply-To: <1ED27760CE36ED4CA7F8B0F090E2AE0D314E2237@SG-MAIL-01.sgmc.org> References: <1ED27760CE36ED4CA7F8B0F090E2AE0D314E2237@SG-MAIL-01.sgmc.org> Message-ID: <1122294465.1865102.1434727449976.JavaMail.yahoo@mail.yahoo.com> TAT is variable but, as a rule, IF the case is slotted for IHC testing?the?slides will be delivered along with the H&E early next day (if received after 4PM) or by late afternoon the same day (if received before 10AM)Ren? On Friday, June 19, 2015 11:11 AM, "Olszewski, Dawn" wrote: Hi Histonetters,? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? 6-19-15 We are wondering what the average turnaround times for IHC are for other labs.? We use the Biocare Intellipath instrumentation. It holds up to 50 slides ( 10 slides per 5 racks) with continuous feed per open rack.? All IHC orders placed by 12pm are usually out the same day by 5pm. We average 434 IHC slides per month and have a staff of 3 FTE's. A pathologist has voiced concerns over our IHC output.? We are trying to determine "best practices" for IHC turnaround time as measured from time order placed to time of slide delivery. If you could respond with your IHC TAT including number of techs and average of IHC slides monthly, we would appreciate any input you may be able to provide. Thank you in advance, Dawn Olszewski, HTL(ASCP)QIHC _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cjohnson at nmda.nmsu.edu Fri Jun 19 10:27:11 2015 From: cjohnson at nmda.nmsu.edu (Johnson, Carole) Date: Fri, 19 Jun 2015 15:27:11 +0000 Subject: [Histonet] Charging for IHC and special stains - veterinary Message-ID: Good morning. Before you read the rest of this post, realize that I work in a veterinary lab and billing is a different animal (no pun intended). Our SOPs require the pathologists to contact the referring clinician prior to ordering special stains or IHC, and this must be documented in the pathology report. This is in accordance with audits from an external (certifying) agency. How are other vet labs complying with this? Any suggestions are greatly appreciated. Happy Friday, Carole Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From SPINHEIRO at lumc.edu Fri Jun 19 10:54:03 2015 From: SPINHEIRO at lumc.edu (STEVEN PINHEIRO) Date: Fri, 19 Jun 2015 15:54:03 +0000 Subject: [Histonet] IHC turnaround time In-Reply-To: <1ED27760CE36ED4CA7F8B0F090E2AE0D314E2237@SG-MAIL-01.sgmc.org> References: <1ED27760CE36ED4CA7F8B0F090E2AE0D314E2237@SG-MAIL-01.sgmc.org> Message-ID: Dawn, TAT has many variables. We run mostly Leica Bond platforms, but do have some Ventana Benchmark access if needed. The average volume here about 125 slides a day. The available time is directly dependent or order protocols. If the order is available before 11 am, the slides will be available mid to late afternoon the same day. If the order is created between 11 am and 4 pm, the slides will be available late that same evening (but usually not reviewed by pathology until the next morning). Order placed after 4 pm are variable and may make the evening run but will most likely not be run until the next day. We do not have an overnight run. Steve Pinheiro 708-327-2642 -----Original Message----- From: Olszewski, Dawn [mailto:Dawn.Olszewski at SGMC.ORG] Sent: Friday, June 19, 2015 9:53 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC turnaround time Hi Histonetters, 6-19-15 We are wondering what the average turnaround times for IHC are for other labs. We use the Biocare Intellipath instrumentation. It holds up to 50 slides ( 10 slides per 5 racks) with continuous feed per open rack. All IHC orders placed by 12pm are usually out the same day by 5pm. We average 434 IHC slides per month and have a staff of 3 FTE's. A pathologist has voiced concerns over our IHC output. We are trying to determine "best practices" for IHC turnaround time as measured from time order placed to time of slide delivery. If you could respond with your IHC TAT including number of techs and average of IHC slides monthly, we would appreciate any input you may be able to provide. Thank you in advance, Dawn Olszewski, HTL(ASCP)QIHC _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Richard.Cartun at hhchealth.org Fri Jun 19 11:09:10 2015 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 19 Jun 2015 16:09:10 +0000 Subject: [Histonet] FW: IHC turnaround time In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E3EE9E685@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E3EE9E685@HHCEXCHMB03.hhcsystem.org> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E3EE9E6E3@HHCEXCHMB03.hhcsystem.org> I apologize; I meant to preface my remarks with, "We have separate Histology and IHC Labs". My data may prove more useful to IHC only labs. Richard -----Original Message----- From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] Sent: Friday, June 19, 2015 11:21 AM To: Olszewski, Dawn; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC turnaround time I direct a busy and very efficient (I think) IHC lab staffed with 2.875 FTEs who spend most of their time doing IHC, but they also help Histology embed and cut paraffin blocks when needed (which is most days). For 2015 we are averaging 4,022 IHC patient test slides and 1,650 IHC control slides per month. Our IHC lab is staffed from 4:30 AM to 6 PM (or later depending on workload). We have 5 Leica Bond Max platforms; however, we still do some IHCs on the bench. We usually do 2-3 runs per day and an overnight run (except on Fridays) as well. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Olszewski, Dawn [mailto:Dawn.Olszewski at SGMC.ORG] Sent: Friday, June 19, 2015 10:53 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC turnaround time Hi Histonetters, 6-19-15 We are wondering what the average turnaround times for IHC are for other labs. We use the Biocare Intellipath instrumentation. It holds up to 50 slides ( 10 slides per 5 racks) with continuous feed per open rack. All IHC orders placed by 12pm are usually out the same day by 5pm. We average 434 IHC slides per month and have a staff of 3 FTE's. A pathologist has voiced concerns over our IHC output. We are trying to determine "best practices" for IHC turnaround time as measured from time order placed to time of slide delivery. If you could respond with your IHC TAT including number of techs and average of IHC slides monthly, we would appreciate any input you may be able to provide. Thank you in advance, Dawn Olszewski, HTL(ASCP)QIHC _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cjbulmer2526 at aol.com Fri Jun 19 11:41:04 2015 From: cjbulmer2526 at aol.com (Cindy Bulmer) Date: Fri, 19 Jun 2015 12:41:04 -0400 Subject: [Histonet] IHC Survey Message-ID: <14e0cb1f0b4-6dda-880b@webprd-a45.mail.aol.com> Hello Histonet, Taking a survey, Do you..... 1) Place the positive control section on the PT slide? 2) Position of the positive control section on the slide with the PT section. 3) Pre-cut positive controls to be placed on the PT slide? 4) Storage of positive controls pre-cut (with PT section). 5) Use a bar code / tracking system? Thank you so much for your time on this matter, Cindy Cindy Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX From mjdessoye at commonwealthhealth.net Fri Jun 19 11:40:25 2015 From: mjdessoye at commonwealthhealth.net (Dessoye, Michael) Date: Fri, 19 Jun 2015 16:40:25 +0000 Subject: [Histonet] Microscope Message-ID: Can anyone out there recommend a microscope (or at least supplier) that they like? I'm in need of a teaching scope with 3 heads and preferably video capability. Any recommendations are appreciated! Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From jaylundgren at gmail.com Fri Jun 19 13:04:25 2015 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 19 Jun 2015 13:04:25 -0500 Subject: [Histonet] IHC turnaround time In-Reply-To: References: <1ED27760CE36ED4CA7F8B0F090E2AE0D314E2237@SG-MAIL-01.sgmc.org> Message-ID: Let me get this straight, Dawn. You are running "In by 12, out by 5." on IHCs and your pathologist is* complaining*? A 5 hour TAT is awesome! Most places I've worked (and I've seen a lot in 16 years as a traveling tech) have at least a 24 hour TAT for IHC. I've got to get a shirt to the cleaners before 9 to get it back by 5! Which is more complex, washing a shirt, or doing IHC on human tissue?! Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Jun 19, 2015 at 10:54 AM, STEVEN PINHEIRO wrote: > Dawn, > TAT has many variables. We run mostly Leica Bond platforms, but do have > some Ventana Benchmark access if needed. The average volume here about 125 > slides a day. The available time is directly dependent or order protocols. > If the order is available before 11 am, the slides will be available mid to > late afternoon the same day. If the order is created between 11 am and 4 > pm, the slides will be available late that same evening (but usually not > reviewed by pathology until the next morning). Order placed after 4 pm are > variable and may make the evening run but will most likely not be run until > the next day. We do not have an overnight run. > > Steve Pinheiro > 708-327-2642 > > > -----Original Message----- > From: Olszewski, Dawn [mailto:Dawn.Olszewski at SGMC.ORG] > Sent: Friday, June 19, 2015 9:53 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] IHC turnaround time > > Hi Histonetters, > > 6-19-15 > > We are wondering what the average turnaround times for IHC are for other > labs. We use the Biocare Intellipath instrumentation. It holds up to 50 > slides ( 10 slides per 5 racks) with continuous feed per open rack. All > IHC orders placed by 12pm are usually out the same day by 5pm. We average > 434 IHC slides per month and have a staff of 3 FTE's. > > A pathologist has voiced concerns over our IHC output. We are trying to > determine "best practices" for IHC turnaround time as measured from time > order placed to time of slide delivery. > > If you could respond with your IHC TAT including number of techs and > average of IHC slides monthly, we would appreciate any input you may be > able to provide. > > Thank you in advance, > > Dawn Olszewski, HTL(ASCP)QIHC > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa at yahoo.com Fri Jun 19 15:31:23 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 19 Jun 2015 20:31:23 +0000 (UTC) Subject: [Histonet] Microscope In-Reply-To: References: Message-ID: <584356349.2123494.1434745883983.JavaMail.yahoo@mail.yahoo.com> New, from Leica (Leitz).Used, there are great microscopes at bargain prices in eBay. Check them out!" Ren? On Friday, June 19, 2015 12:42 PM, "Dessoye, Michael" wrote: Can anyone out there recommend a microscope (or at least supplier) that they like?? I'm in need of a teaching scope with 3 heads and preferably video capability.? Any recommendations are appreciated! Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dunatrsd at sbcglobal.net Fri Jun 19 16:43:31 2015 From: dunatrsd at sbcglobal.net (dusko trajkovic) Date: Fri, 19 Jun 2015 21:43:31 +0000 (UTC) Subject: [Histonet] Looking for a used tissue processor Message-ID: <717483181.2139151.1434750211217.JavaMail.yahoo@mail.yahoo.com> I'm in a market for a used tissue processor, preferably a ?Sakura VIP 5. I am located in San Diego area.Please contact me at ?dunatrsd at sbcglobal.net 858-638-6202ThanksDusko From LSebree at uwhealth.org Fri Jun 19 16:47:58 2015 From: LSebree at uwhealth.org (Sebree Linda A) Date: Fri, 19 Jun 2015 21:47:58 +0000 Subject: [Histonet] IHC turnaround time In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E3EE9E685@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E3EE9E685@HHCEXCHMB03.hhcsystem.org> Message-ID: <77DD817201982748BC67D7960F2F76AF148A79@UWHC-MBX12.uwhis.hosp.wisc.edu> We have 2 FTEs in IHC; myself as one of two people that started the lab 20+ years ago and 2 histotechs that rotate through on a weekly basis. Our monthly totals currently run between 2400 and 3300. Our TAT policy is "in by noon, out by 5pm" except for dual and triple stains (need to be on an instrument by 11:00 am) and EBER (needs to be on by ~ 9:00 am) and HER2 dual ISH (only run overnight). We have 4 Roche (Ventana) Ultras that hold 30 slides each and are continuous access. All instruments are used every day and at least one (usually 2 or 3) in use overnight. Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] Sent: Friday, June 19, 2015 10:21 AM To: Olszewski, Dawn; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC turnaround time I direct a busy and very efficient (I think) IHC lab staffed with 2.875 FTEs who spend most of their time doing IHC, but they also help Histology embed and cut paraffin blocks when needed (which is most days). For 2015 we are averaging 4,022 IHC patient test slides and 1,650 IHC control slides per month. Our IHC lab is staffed from 4:30 AM to 6 PM (or later depending on workload). We have 5 Leica Bond Max platforms; however, we still do some IHCs on the bench. We usually do 2-3 runs per day and an overnight run (except on Fridays) as well. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Olszewski, Dawn [mailto:Dawn.Olszewski at SGMC.ORG] Sent: Friday, June 19, 2015 10:53 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC turnaround time Hi Histonetters, 6-19-15 We are wondering what the average turnaround times for IHC are for other labs. We use the Biocare Intellipath instrumentation. It holds up to 50 slides ( 10 slides per 5 racks) with continuous feed per open rack. All IHC orders placed by 12pm are usually out the same day by 5pm. We average 434 IHC slides per month and have a staff of 3 FTE's. A pathologist has voiced concerns over our IHC output. We are trying to determine "best practices" for IHC turnaround time as measured from time order placed to time of slide delivery. If you could respond with your IHC TAT including number of techs and average of IHC slides monthly, we would appreciate any input you may be able to provide. Thank you in advance, Dawn Olszewski, HTL(ASCP)QIHC _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree at uwhealth.org Fri Jun 19 16:51:00 2015 From: LSebree at uwhealth.org (Sebree Linda A) Date: Fri, 19 Jun 2015 21:51:00 +0000 Subject: [Histonet] IHC turnaround time In-Reply-To: References: <1ED27760CE36ED4CA7F8B0F090E2AE0D314E2237@SG-MAIL-01.sgmc.org> Message-ID: <77DD817201982748BC67D7960F2F76AF148A8F@UWHC-MBX12.uwhis.hosp.wisc.edu> I agree Jay. We have the same TAT policy and it's "kick ass" every single day to achieve this with never enough time to cut controls, check out new lots, bring on new antibodies, etc. Our staffing situation will need to change pretty soon, especially as I look towards retirement :). Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Jay Lundgren [mailto:jaylundgren at gmail.com] Sent: Friday, June 19, 2015 1:04 PM To: STEVEN PINHEIRO Cc: histonet at lists.utsouthwestern.edu; Olszewski, Dawn Subject: Re: [Histonet] IHC turnaround time Let me get this straight, Dawn. You are running "In by 12, out by 5." on IHCs and your pathologist is* complaining*? A 5 hour TAT is awesome! Most places I've worked (and I've seen a lot in 16 years as a traveling tech) have at least a 24 hour TAT for IHC. I've got to get a shirt to the cleaners before 9 to get it back by 5! Which is more complex, washing a shirt, or doing IHC on human tissue?! Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Jun 19, 2015 at 10:54 AM, STEVEN PINHEIRO wrote: > Dawn, > TAT has many variables. We run mostly Leica Bond platforms, but do > have some Ventana Benchmark access if needed. The average volume here > about 125 slides a day. The available time is directly dependent or order protocols. > If the order is available before 11 am, the slides will be available > mid to late afternoon the same day. If the order is created between 11 > am and 4 pm, the slides will be available late that same evening (but > usually not reviewed by pathology until the next morning). Order > placed after 4 pm are variable and may make the evening run but will > most likely not be run until the next day. We do not have an overnight run. > > Steve Pinheiro > 708-327-2642 > > > -----Original Message----- > From: Olszewski, Dawn [mailto:Dawn.Olszewski at SGMC.ORG] > Sent: Friday, June 19, 2015 9:53 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] IHC turnaround time > > Hi Histonetters, > > 6-19-15 > > We are wondering what the average turnaround times for IHC are for > other labs. We use the Biocare Intellipath instrumentation. It holds > up to 50 slides ( 10 slides per 5 racks) with continuous feed per open > rack. All IHC orders placed by 12pm are usually out the same day by > 5pm. We average > 434 IHC slides per month and have a staff of 3 FTE's. > > A pathologist has voiced concerns over our IHC output. We are trying > to determine "best practices" for IHC turnaround time as measured from > time order placed to time of slide delivery. > > If you could respond with your IHC TAT including number of techs and > average of IHC slides monthly, we would appreciate any input you may > be able to provide. > > Thank you in advance, > > Dawn Olszewski, HTL(ASCP)QIHC > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity > Health and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.rowaihi at alborglaboratories.com Sat Jun 20 09:07:37 2015 From: j.rowaihi at alborglaboratories.com (Jamal) Date: Sat, 20 Jun 2015 17:07:37 +0300 Subject: [Histonet] competency assessment for Anatomic Pathologist Message-ID: <01e601d0ab62$7c6c4740$7544d5c0$@rowaihi@alborglaboratories.com> Dear colleagues I hope if any one share me the form of: competency assessment for Anatomic Pathologist Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi at alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com From rascal_40 at hotmail.com Sat Jun 20 21:48:24 2015 From: rascal_40 at hotmail.com (Karen Stephanson) Date: Sat, 20 Jun 2015 20:48:24 -0600 Subject: [Histonet] (no subject) Message-ID: Our Histology laboratory currently uses Mercurochrome to mark specimens, but we need to discontinue using it.? Can anybody tell me of a good substitute.? From susanhay59 at gmail.com Sat Jun 20 22:10:13 2015 From: susanhay59 at gmail.com (S hay) Date: Sat, 20 Jun 2015 22:10:13 -0500 Subject: [Histonet] competency assessment for Anatomic Pathologist In-Reply-To: <558576d5.c1e7b60a.2769.ffffe9d0SMTPIN_ADDED_BROKEN@mx.google.com> References: <558576d5.c1e7b60a.2769.ffffe9d0SMTPIN_ADDED_BROKEN@mx.google.com> Message-ID: Please post here. I am interested as well. On Jun 20, 2015 9:21 AM, "Jamal" wrote: > Dear colleagues > > I hope if any one share me the form of: > > competency assessment for Anatomic Pathologist > > > > > > Best Regards, > > > > > > Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg > Medical Laboratories | Mobile +966 503629832| > j.rowaihi at alborglaboratories.com > > Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | > Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | > www.alborglaboratories.com > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From DKBoyd at chs.net Mon Jun 22 06:39:23 2015 From: DKBoyd at chs.net (Boyd, Debbie M) Date: Mon, 22 Jun 2015 11:39:23 +0000 Subject: [Histonet] [EXTERNAL] Re: competency assessment for Anatomic Pathologist In-Reply-To: References: <558576d5.c1e7b60a.2769.ffffe9d0SMTPIN_ADDED_BROKEN@mx.google.com>, Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9F37C5F@TN001WEXMBX014.US.chs.net> Our pathologist are evaluated using the OPPE ( Ongoing Physician Performance Evaluation) method. In that method we monitor TAT for Surg. Path, Cyto, Autop. Frozen Sections, Bone Marrows. Cyto to Histo correlations, frozen to permanent correlations, Post OP to Final correlations. Peer Review cases agreements vs. disagreements. The below information is formated on an Excel spreadsheet. This information is reported quarterly to our QA department. They take care of the rest for re-credentialing. Cases Reviewed Frozen Section/Histology Correlation Cytology/Histology Correlation Autopsies Preliminary Autopsy Report completed within 72 hrs Final Autopsy Report completed within 60 Days Bone Marrow Aspiration Reviews INTERNAL REVIEWS External Consultations 1-No difference in interpretations 2-Difference in interpretationw w/no potential impact 3-Difference in interpretation w/minimal potential impact 4-Difference in interpretation w/significant potential for altering plan of care Surgical Turnaround Study- #Cases Sampled <24 hours <48 hours <72 hours Decals, BM, Send Outs >96 hours Percentage Completed Within 48 hours CytologyTurnaround Study- #Cases Sampled <24 hours <48 hours <72 hours >96 hours Percentage Completed Within 48 hours Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: S hay [susanhay59 at gmail.com] Sent: Saturday, June 20, 2015 11:10 PM To: Jamal Cc: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] competency assessment for Anatomic Pathologist Please post here. I am interested as well. On Jun 20, 2015 9:21 AM, "Jamal" wrote: > Dear colleagues > > I hope if any one share me the form of: > > competency assessment for Anatomic Pathologist > > > > > > Best Regards, > > > > > > Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg > Medical Laboratories | Mobile +966 503629832| > j.rowaihi at alborglaboratories.com > > Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | > Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | > www.alborglaboratories.com > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From bcdukes at lexhealth.org Mon Jun 22 07:29:57 2015 From: bcdukes at lexhealth.org (Blake Taylor) Date: Mon, 22 Jun 2015 12:29:57 +0000 Subject: [Histonet] Competency assessment for PA's In-Reply-To: References: Message-ID: Would anyone be willing to share your competency assessment for PA's Thanks so much Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes at lexhealth.org -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Saturday, June 20, 2015 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 139, Issue 20 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: IHC turnaround time (Jay Lundgren) 2. Re: Microscope (Rene J Buesa) 3. Looking for a used tissue processor (dusko trajkovic) 4. Re: IHC turnaround time (Sebree Linda A) 5. Re: IHC turnaround time (Sebree Linda A) 6. competency assessment for Anatomic Pathologist (Jamal) ---------------------------------------------------------------------- Message: 1 Date: Fri, 19 Jun 2015 13:04:25 -0500 From: Jay Lundgren To: STEVEN PINHEIRO Cc: "Olszewski, Dawn" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] IHC turnaround time Message-ID: Content-Type: text/plain; charset=UTF-8 Let me get this straight, Dawn. You are running "In by 12, out by 5." on IHCs and your pathologist is* complaining*? A 5 hour TAT is awesome! Most places I've worked (and I've seen a lot in 16 years as a traveling tech) have at least a 24 hour TAT for IHC. I've got to get a shirt to the cleaners before 9 to get it back by 5! Which is more complex, washing a shirt, or doing IHC on human tissue?! Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Jun 19, 2015 at 10:54 AM, STEVEN PINHEIRO wrote: > Dawn, > TAT has many variables. We run mostly Leica Bond platforms, but do have > some Ventana Benchmark access if needed. The average volume here about 125 > slides a day. The available time is directly dependent or order protocols. > If the order is available before 11 am, the slides will be available mid to > late afternoon the same day. If the order is created between 11 am and 4 > pm, the slides will be available late that same evening (but usually not > reviewed by pathology until the next morning). Order placed after 4 pm are > variable and may make the evening run but will most likely not be run until > the next day. We do not have an overnight run. > > Steve Pinheiro > 708-327-2642 > > > -----Original Message----- > From: Olszewski, Dawn [mailto:Dawn.Olszewski at SGMC.ORG] > Sent: Friday, June 19, 2015 9:53 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] IHC turnaround time > > Hi Histonetters, > > 6-19-15 > > We are wondering what the average turnaround times for IHC are for other > labs. We use the Biocare Intellipath instrumentation. It holds up to 50 > slides ( 10 slides per 5 racks) with continuous feed per open rack. All > IHC orders placed by 12pm are usually out the same day by 5pm. We average > 434 IHC slides per month and have a staff of 3 FTE's. > > A pathologist has voiced concerns over our IHC output. We are trying to > determine "best practices" for IHC turnaround time as measured from time > order placed to time of slide delivery. > > If you could respond with your IHC TAT including number of techs and > average of IHC slides monthly, we would appreciate any input you may be > able to provide. > > Thank you in advance, > > Dawn Olszewski, HTL(ASCP)QIHC > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 2 Date: Fri, 19 Jun 2015 20:31:23 +0000 (UTC) From: Rene J Buesa To: "Dessoye, Michael" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Microscope Message-ID: <584356349.2123494.1434745883983.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 New, from Leica (Leitz).Used, there are great microscopes at bargain prices in eBay. Check them out!" Ren? On Friday, June 19, 2015 12:42 PM, "Dessoye, Michael" wrote: Can anyone out there recommend a microscope (or at least supplier) that they like?? I'm in need of a teaching scope with 3 heads and preferably video capability.? Any recommendations are appreciated! Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 19 Jun 2015 21:43:31 +0000 (UTC) From: dusko trajkovic To: Histonet Subject: [Histonet] Looking for a used tissue processor Message-ID: <717483181.2139151.1434750211217.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 I'm in a market for a used tissue processor, preferably a ?Sakura VIP 5. I am located in San Diego area.Please contact me at ?dunatrsd at sbcglobal.net 858-638-6202ThanksDusko ------------------------------ Message: 4 Date: Fri, 19 Jun 2015 21:47:58 +0000 From: Sebree Linda A To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] IHC turnaround time Message-ID: <77DD817201982748BC67D7960F2F76AF148A79 at UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" We have 2 FTEs in IHC; myself as one of two people that started the lab 20+ years ago and 2 histotechs that rotate through on a weekly basis. Our monthly totals currently run between 2400 and 3300. Our TAT policy is "in by noon, out by 5pm" except for dual and triple stains (need to be on an instrument by 11:00 am) and EBER (needs to be on by ~ 9:00 am) and HER2 dual ISH (only run overnight). We have 4 Roche (Ventana) Ultras that hold 30 slides each and are continuous access. All instruments are used every day and at least one (usually 2 or 3) in use overnight. Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] Sent: Friday, June 19, 2015 10:21 AM To: Olszewski, Dawn; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC turnaround time I direct a busy and very efficient (I think) IHC lab staffed with 2.875 FTEs who spend most of their time doing IHC, but they also help Histology embed and cut paraffin blocks when needed (which is most days). For 2015 we are averaging 4,022 IHC patient test slides and 1,650 IHC control slides per month. Our IHC lab is staffed from 4:30 AM to 6 PM (or later depending on workload). We have 5 Leica Bond Max platforms; however, we still do some IHCs on the bench. We usually do 2-3 runs per day and an overnight run (except on Fridays) as well. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Olszewski, Dawn [mailto:Dawn.Olszewski at SGMC.ORG] Sent: Friday, June 19, 2015 10:53 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC turnaround time Hi Histonetters, 6-19-15 We are wondering what the average turnaround times for IHC are for other labs. We use the Biocare Intellipath instrumentation. It holds up to 50 slides ( 10 slides per 5 racks) with continuous feed per open rack. All IHC orders placed by 12pm are usually out the same day by 5pm. We average 434 IHC slides per month and have a staff of 3 FTE's. A pathologist has voiced concerns over our IHC output. We are trying to determine "best practices" for IHC turnaround time as measured from time order placed to time of slide delivery. If you could respond with your IHC TAT including number of techs and average of IHC slides monthly, we would appreciate any input you may be able to provide. Thank you in advance, Dawn Olszewski, HTL(ASCP)QIHC _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 19 Jun 2015 21:51:00 +0000 From: Sebree Linda A To: 'Jay Lundgren' , STEVEN PINHEIRO Cc: "histonet at lists.utsouthwestern.edu" , "Olszewski, Dawn" Subject: Re: [Histonet] IHC turnaround time Message-ID: <77DD817201982748BC67D7960F2F76AF148A8F at UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" I agree Jay. We have the same TAT policy and it's "kick ass" every single day to achieve this with never enough time to cut controls, check out new lots, bring on new antibodies, etc. Our staffing situation will need to change pretty soon, especially as I look towards retirement :). Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Jay Lundgren [mailto:jaylundgren at gmail.com] Sent: Friday, June 19, 2015 1:04 PM To: STEVEN PINHEIRO Cc: histonet at lists.utsouthwestern.edu; Olszewski, Dawn Subject: Re: [Histonet] IHC turnaround time Let me get this straight, Dawn. You are running "In by 12, out by 5." on IHCs and your pathologist is* complaining*? A 5 hour TAT is awesome! Most places I've worked (and I've seen a lot in 16 years as a traveling tech) have at least a 24 hour TAT for IHC. I've got to get a shirt to the cleaners before 9 to get it back by 5! Which is more complex, washing a shirt, or doing IHC on human tissue?! Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Jun 19, 2015 at 10:54 AM, STEVEN PINHEIRO wrote: > Dawn, > TAT has many variables. We run mostly Leica Bond platforms, but do > have some Ventana Benchmark access if needed. The average volume here > about 125 slides a day. The available time is directly dependent or order protocols. > If the order is available before 11 am, the slides will be available > mid to late afternoon the same day. If the order is created between 11 > am and 4 pm, the slides will be available late that same evening (but > usually not reviewed by pathology until the next morning). Order > placed after 4 pm are variable and may make the evening run but will > most likely not be run until the next day. We do not have an overnight run. > > Steve Pinheiro > 708-327-2642 > > > -----Original Message----- > From: Olszewski, Dawn [mailto:Dawn.Olszewski at SGMC.ORG] > Sent: Friday, June 19, 2015 9:53 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] IHC turnaround time > > Hi Histonetters, > > 6-19-15 > > We are wondering what the average turnaround times for IHC are for > other labs. We use the Biocare Intellipath instrumentation. It holds > up to 50 slides ( 10 slides per 5 racks) with continuous feed per open > rack. All IHC orders placed by 12pm are usually out the same day by > 5pm. We average > 434 IHC slides per month and have a staff of 3 FTE's. > > A pathologist has voiced concerns over our IHC output. We are trying > to determine "best practices" for IHC turnaround time as measured from > time order placed to time of slide delivery. > > If you could respond with your IHC TAT including number of techs and > average of IHC slides monthly, we would appreciate any input you may > be able to provide. > > Thank you in advance, > > Dawn Olszewski, HTL(ASCP)QIHC > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity > Health and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Sat, 20 Jun 2015 17:07:37 +0300 From: "Jamal" To: Subject: [Histonet] competency assessment for Anatomic Pathologist Message-ID: <01e601d0ab62$7c6c4740$7544d5c0$@rowaihi at alborglaboratories.com> Content-Type: text/plain; charset="us-ascii" Dear colleagues I hope if any one share me the form of: competency assessment for Anatomic Pathologist Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi at alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 139, Issue 20 ***************************************** PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. From Joyce.Weems at emoryhealthcare.org Mon Jun 22 08:27:59 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Mon, 22 Jun 2015 13:27:59 +0000 Subject: [Histonet] Competency assessment for PA's In-Reply-To: References: Message-ID: Me too, me too!! Thanks Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Blake Taylor [mailto:bcdukes at lexhealth.org] Sent: Monday, June 22, 2015 8:30 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Competency assessment for PA's Would anyone be willing to share your competency assessment for PA's Thanks so much Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes at lexhealth.org -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Saturday, June 20, 2015 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 139, Issue 20 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: IHC turnaround time (Jay Lundgren) 2. Re: Microscope (Rene J Buesa) 3. Looking for a used tissue processor (dusko trajkovic) 4. Re: IHC turnaround time (Sebree Linda A) 5. Re: IHC turnaround time (Sebree Linda A) 6. competency assessment for Anatomic Pathologist (Jamal) ---------------------------------------------------------------------- Message: 1 Date: Fri, 19 Jun 2015 13:04:25 -0500 From: Jay Lundgren To: STEVEN PINHEIRO Cc: "Olszewski, Dawn" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] IHC turnaround time Message-ID: Content-Type: text/plain; charset=UTF-8 Let me get this straight, Dawn. You are running "In by 12, out by 5." on IHCs and your pathologist is* complaining*? A 5 hour TAT is awesome! Most places I've worked (and I've seen a lot in 16 years as a traveling tech) have at least a 24 hour TAT for IHC. I've got to get a shirt to the cleaners before 9 to get it back by 5! Which is more complex, washing a shirt, or doing IHC on human tissue?! Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Jun 19, 2015 at 10:54 AM, STEVEN PINHEIRO wrote: > Dawn, > TAT has many variables. We run mostly Leica Bond platforms, but do > have some Ventana Benchmark access if needed. The average volume here > about 125 slides a day. The available time is directly dependent or order protocols. > If the order is available before 11 am, the slides will be available > mid to late afternoon the same day. If the order is created between 11 > am and 4 pm, the slides will be available late that same evening (but > usually not reviewed by pathology until the next morning). Order > placed after 4 pm are variable and may make the evening run but will > most likely not be run until the next day. We do not have an overnight run. > > Steve Pinheiro > 708-327-2642 > > > -----Original Message----- > From: Olszewski, Dawn [mailto:Dawn.Olszewski at SGMC.ORG] > Sent: Friday, June 19, 2015 9:53 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] IHC turnaround time > > Hi Histonetters, > > 6-19-15 > > We are wondering what the average turnaround times for IHC are for > other labs. We use the Biocare Intellipath instrumentation. It holds > up to 50 slides ( 10 slides per 5 racks) with continuous feed per open > rack. All IHC orders placed by 12pm are usually out the same day by > 5pm. We average > 434 IHC slides per month and have a staff of 3 FTE's. > > A pathologist has voiced concerns over our IHC output. We are trying > to determine "best practices" for IHC turnaround time as measured from > time order placed to time of slide delivery. > > If you could respond with your IHC TAT including number of techs and > average of IHC slides monthly, we would appreciate any input you may > be able to provide. > > Thank you in advance, > > Dawn Olszewski, HTL(ASCP)QIHC > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity > Health and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 2 Date: Fri, 19 Jun 2015 20:31:23 +0000 (UTC) From: Rene J Buesa To: "Dessoye, Michael" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Microscope Message-ID: <584356349.2123494.1434745883983.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 New, from Leica (Leitz).Used, there are great microscopes at bargain prices in eBay. Check them out!" Ren? On Friday, June 19, 2015 12:42 PM, "Dessoye, Michael" wrote: Can anyone out there recommend a microscope (or at least supplier) that they like?? I'm in need of a teaching scope with 3 heads and preferably video capability.? Any recommendations are appreciated! Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486 -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 19 Jun 2015 21:43:31 +0000 (UTC) From: dusko trajkovic To: Histonet Subject: [Histonet] Looking for a used tissue processor Message-ID: <717483181.2139151.1434750211217.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 I'm in a market for a used tissue processor, preferably a ?Sakura VIP 5. I am located in San Diego area.Please contact me at ?dunatrsd at sbcglobal.net 858-638-6202ThanksDusko ------------------------------ Message: 4 Date: Fri, 19 Jun 2015 21:47:58 +0000 From: Sebree Linda A To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] IHC turnaround time Message-ID: <77DD817201982748BC67D7960F2F76AF148A79 at UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" We have 2 FTEs in IHC; myself as one of two people that started the lab 20+ years ago and 2 histotechs that rotate through on a weekly basis. Our monthly totals currently run between 2400 and 3300. Our TAT policy is "in by noon, out by 5pm" except for dual and triple stains (need to be on an instrument by 11:00 am) and EBER (needs to be on by ~ 9:00 am) and HER2 dual ISH (only run overnight). We have 4 Roche (Ventana) Ultras that hold 30 slides each and are continuous access. All instruments are used every day and at least one (usually 2 or 3) in use overnight. Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] Sent: Friday, June 19, 2015 10:21 AM To: Olszewski, Dawn; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC turnaround time I direct a busy and very efficient (I think) IHC lab staffed with 2.875 FTEs who spend most of their time doing IHC, but they also help Histology embed and cut paraffin blocks when needed (which is most days). For 2015 we are averaging 4,022 IHC patient test slides and 1,650 IHC control slides per month. Our IHC lab is staffed from 4:30 AM to 6 PM (or later depending on workload). We have 5 Leica Bond Max platforms; however, we still do some IHCs on the bench. We usually do 2-3 runs per day and an overnight run (except on Fridays) as well. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Olszewski, Dawn [mailto:Dawn.Olszewski at SGMC.ORG] Sent: Friday, June 19, 2015 10:53 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC turnaround time Hi Histonetters, 6-19-15 We are wondering what the average turnaround times for IHC are for other labs. We use the Biocare Intellipath instrumentation. It holds up to 50 slides ( 10 slides per 5 racks) with continuous feed per open rack. All IHC orders placed by 12pm are usually out the same day by 5pm. We average 434 IHC slides per month and have a staff of 3 FTE's. A pathologist has voiced concerns over our IHC output. We are trying to determine "best practices" for IHC turnaround time as measured from time order placed to time of slide delivery. If you could respond with your IHC TAT including number of techs and average of IHC slides monthly, we would appreciate any input you may be able to provide. Thank you in advance, Dawn Olszewski, HTL(ASCP)QIHC _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 19 Jun 2015 21:51:00 +0000 From: Sebree Linda A To: 'Jay Lundgren' , STEVEN PINHEIRO Cc: "histonet at lists.utsouthwestern.edu" , "Olszewski, Dawn" Subject: Re: [Histonet] IHC turnaround time Message-ID: <77DD817201982748BC67D7960F2F76AF148A8F at UWHC-MBX12.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" I agree Jay. We have the same TAT policy and it's "kick ass" every single day to achieve this with never enough time to cut controls, check out new lots, bring on new antibodies, etc. Our staffing situation will need to change pretty soon, especially as I look towards retirement :). Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Jay Lundgren [mailto:jaylundgren at gmail.com] Sent: Friday, June 19, 2015 1:04 PM To: STEVEN PINHEIRO Cc: histonet at lists.utsouthwestern.edu; Olszewski, Dawn Subject: Re: [Histonet] IHC turnaround time Let me get this straight, Dawn. You are running "In by 12, out by 5." on IHCs and your pathologist is* complaining*? A 5 hour TAT is awesome! Most places I've worked (and I've seen a lot in 16 years as a traveling tech) have at least a 24 hour TAT for IHC. I've got to get a shirt to the cleaners before 9 to get it back by 5! Which is more complex, washing a shirt, or doing IHC on human tissue?! Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Jun 19, 2015 at 10:54 AM, STEVEN PINHEIRO wrote: > Dawn, > TAT has many variables. We run mostly Leica Bond platforms, but do > have some Ventana Benchmark access if needed. The average volume here > about 125 slides a day. The available time is directly dependent or order protocols. > If the order is available before 11 am, the slides will be available > mid to late afternoon the same day. If the order is created between 11 > am and 4 pm, the slides will be available late that same evening (but > usually not reviewed by pathology until the next morning). Order > placed after 4 pm are variable and may make the evening run but will > most likely not be run until the next day. We do not have an overnight run. > > Steve Pinheiro > 708-327-2642 > > > -----Original Message----- > From: Olszewski, Dawn [mailto:Dawn.Olszewski at SGMC.ORG] > Sent: Friday, June 19, 2015 9:53 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] IHC turnaround time > > Hi Histonetters, > > 6-19-15 > > We are wondering what the average turnaround times for IHC are for > other labs. We use the Biocare Intellipath instrumentation. It holds > up to 50 slides ( 10 slides per 5 racks) with continuous feed per open > rack. All IHC orders placed by 12pm are usually out the same day by > 5pm. We average > 434 IHC slides per month and have a staff of 3 FTE's. > > A pathologist has voiced concerns over our IHC output. We are trying > to determine "best practices" for IHC turnaround time as measured from > time order placed to time of slide delivery. > > If you could respond with your IHC TAT including number of techs and > average of IHC slides monthly, we would appreciate any input you may > be able to provide. > > Thank you in advance, > > Dawn Olszewski, HTL(ASCP)QIHC > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity > Health and is intended for the sole use of the intended recipient(s). > It may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. > If you are not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Sat, 20 Jun 2015 17:07:37 +0300 From: "Jamal" To: Subject: [Histonet] competency assessment for Anatomic Pathologist Message-ID: <01e601d0ab62$7c6c4740$7544d5c0$@rowaihi at alborglaboratories.com> Content-Type: text/plain; charset="us-ascii" Dear colleagues I hope if any one share me the form of: competency assessment for Anatomic Pathologist Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowaihi at alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 139, Issue 20 ***************************************** PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From anna.coffey at nih.gov Mon Jun 22 09:24:50 2015 From: anna.coffey at nih.gov (Coffey, Anna (NIH/NCI) [C]) Date: Mon, 22 Jun 2015 14:24:50 +0000 Subject: [Histonet] Removing paraffin sections from glass slides Message-ID: <5C3E10119A1B824FBE92B08279F74A91025973BC@msgb10.nih.gov> Hello, I'm wondering if anyone has experience removing dried unstained paraffin sections from charged glass slides. I don't need to preserve the sections, just want to reuse the slides. Thanks! Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey at nih.gov 301-846-1730 From Joyce.Weems at emoryhealthcare.org Mon Jun 22 09:52:30 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Mon, 22 Jun 2015 14:52:30 +0000 Subject: [Histonet] Removing paraffin sections from glass slides In-Reply-To: <5C3E10119A1B824FBE92B08279F74A91025973BC@msgb10.nih.gov> References: <5C3E10119A1B824FBE92B08279F74A91025973BC@msgb10.nih.gov> Message-ID: This is what I would do... Soak the coverslip off in xylene Either - rehydrate back to water and just wipe the tissue off - then dip the slides in absolute and let dry.. OR - air dry out of xylene and wipe off with a wet gauze - then do the alcohol dip Whichever worked best. Just my 2 cents.. Happy Monday!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Coffey, Anna (NIH/NCI) [C] [mailto:anna.coffey at nih.gov] Sent: Monday, June 22, 2015 10:25 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Removing paraffin sections from glass slides Hello, I'm wondering if anyone has experience removing dried unstained paraffin sections from charged glass slides. I don't need to preserve the sections, just want to reuse the slides. Thanks! Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey at nih.gov 301-846-1730 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From rachel at gbi-inc.com Mon Jun 22 16:20:56 2015 From: rachel at gbi-inc.com (Rachel M Gonzalez) Date: Mon, 22 Jun 2015 17:20:56 -0400 Subject: [Histonet] de-waxing protocol on instruments just fact finding Message-ID: Hi Just beginning to look at instruments DAKO Ventana Leica. We may not have the budget but if we do.... However talking to the sales reps have been challenging as everything is proprietary. This is for research consideration so we will use an open instrument? Which instrument do you like better open? Does manual dewaxing affect the staining? Every rep says yes... but does it? What is the time for dewaxing when you start the instrument? What are the number of steps in the protocol? What is the waste generation like more or less than manual? Thanks for your help. Rachel From naje1972 at yahoo.com Mon Jun 22 21:16:21 2015 From: naje1972 at yahoo.com (cynthia haynes) Date: Mon, 22 Jun 2015 19:16:21 -0700 Subject: [Histonet] de-waxing protocol on instruments just fact finding In-Reply-To: Message-ID: <1435025781.5757.YahooMailAndroidMobile@web160602.mail.bf1.yahoo.com> Only if you don't de-wax long enough.? De-wax for 10 minutes ?in xylene ?at least 3 changes each. Then dehydrate 5 minutes in 100 % ?alcohol 3 changes each. Then 95% alcohol 2 changes each for 5 minutes. Then 80% 2 minutes each and finally 70% 1 minute,then rinse well in DI h2o. hope this helps.? Sent from Yahoo Mail on Android From:"Rachel M Gonzalez" Date:Mon, Jun 22, 2015 at 5:12 PM Subject:[Histonet] de-waxing protocol on instruments just fact finding Hi Just beginning to look at instruments DAKO Ventana Leica. We may not have the budget but if we do.... However talking to the sales reps have been challenging as everything is proprietary. This is for research consideration so we will use an open instrument? Which instrument do you like better open? Does? manual dewaxing affect the staining? Every rep says yes... but does it? What is the time for dewaxing when you start the instrument? What are the number of steps in the protocol? What is the waste generation like more or less than manual? Thanks for your help. Rachel _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.rowaihi at alborglaboratories.com Tue Jun 23 04:56:02 2015 From: j.rowaihi at alborglaboratories.com (Jamal) Date: Tue, 23 Jun 2015 12:56:02 +0300 Subject: [Histonet] Removing paraffin sections from glass slides In-Reply-To: References: <5C3E10119A1B824FBE92B08279F74A91025973BC@msgb10.nih.gov> Message-ID: <028801d0ad9a$d63d8830$82b89890$@rowaihi@alborglaboratories.com> Dear Colleague I just want to remind you.. After all of that procedure of removing the paraffin sections from the positive charged slides. The slides will not be positive charged anymore and not suitable for IHC. Also the cost of the detergents and the chemicals which you used for removing the tissue section is more costly than the slides. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi at alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] Sent: Monday, June 22, 2015 5:53 PM To: 'Coffey, Anna (NIH/NCI) [C]'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Removing paraffin sections from glass slides This is what I would do... Soak the coverslip off in xylene Either - rehydrate back to water and just wipe the tissue off - then dip the slides in absolute and let dry.. OR - air dry out of xylene and wipe off with a wet gauze - then do the alcohol dip Whichever worked best. Just my 2 cents.. Happy Monday!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Coffey, Anna (NIH/NCI) [C] [mailto:anna.coffey at nih.gov] Sent: Monday, June 22, 2015 10:25 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Removing paraffin sections from glass slides Hello, I'm wondering if anyone has experience removing dried unstained paraffin sections from charged glass slides. I don't need to preserve the sections, just want to reuse the slides. Thanks! Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey at nih.gov 301-846-1730 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek at digestivespecialists.com Tue Jun 23 06:37:12 2015 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Tue, 23 Jun 2015 07:37:12 -0400 Subject: [Histonet] de-waxing protocol on instruments just fact finding In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E3917427152D7@IBMB7Exchange.digestivespecialists.com> Rachel, We dewax off line. We use the same protocol that we do for any other staining; 3 min in 3 changes of xylene (we use a xylene substitute though), 3 min in 2 changes of 100% alcohol and one 3 min in 95%. We use BioCare's IntelliPath immuno stainer. It is a completely open system. All of the sales reps, support techs, and service tech have always been very open and willing to help in any way to provide information and support for this stainer. If by dewaxing you are also referring to off line antigen retrieval, we use the pressure cooker that BioCare has. You can use any retrieval solution you choose with it. If you need to use two different retrieval solutions you can fill your containers with two different retrieval reagents. I don't find that there is any difference in doing off line retrieval than on line retrieval. If I can answer any other questions please feel free to contact me. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Rachel M Gonzalez [mailto:rachel at gbi-inc.com] Sent: Monday, June 22, 2015 5:21 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] de-waxing protocol on instruments just fact finding Hi Just beginning to look at instruments DAKO Ventana Leica. We may not have the budget but if we do.... However talking to the sales reps have been challenging as everything is proprietary. This is for research consideration so we will use an open instrument? Which instrument do you like better open? Does manual dewaxing affect the staining? Every rep says yes... but does it? What is the time for dewaxing when you start the instrument? What are the number of steps in the protocol? What is the waste generation like more or less than manual? Thanks for your help. Rachel _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KSimeone at leavittmgt.com Tue Jun 23 09:27:23 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Tue, 23 Jun 2015 14:27:23 +0000 Subject: [Histonet] FT position HISTOTECHNOLOGIST/IHC Delray Beach, FL In-Reply-To: <43944B1DBAAC2846B7B9D626B5F1233C3DC9AEF8@vm-email.leavittmgt.com> References: <43944B1DBAAC2846B7B9D626B5F1233C3DC9AEF8@vm-email.leavittmgt.com> Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C3DC9C27E@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Dermatology Lab. This is a permanent full time SECOND SHIFT (40 hours) position with benefits (medical/401k/vacation) and competitive pay. THIS IS A DRUG FREE WORKPLACE. Drug testing, background check and personality testing is required. ONLY SERIOUS INQURIES, please read EVERY qualification desired. Sorry, NO relocation assistance offered. Position available for training immediately. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengimann at leavittmgt.com if interested. *Full time position Mon-Fri 2p-10:30p (IMMUNOHISTOCHEMISTRY/SPECIALS department) *MUST be licensed as a FLORIDA HISTOTECHNOLOGIST (this is NOT negotiable) *EXPERIENCE WITH IHC A MUST! Leica (BOND) and Roche/Ventana equipment experience preferred *Must be able to multi-task special stains *MUST have at LEAST 2 years experience. Please DO NOT respond if you do not possess EXPERIENCE in this area! *must be confidant, quick learner, self motivated, reliable and a team player Kari M Simeone Histology/Immunohistochemistry Specialist Supervisor The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From Dana.Spencer at vidanthealth.com Tue Jun 23 12:21:24 2015 From: Dana.Spencer at vidanthealth.com (Spencer, Dana) Date: Tue, 23 Jun 2015 17:21:24 +0000 Subject: [Histonet] Specimen Triage Message-ID: <9d2436eaf8d3491580a2029c69b3f954@WSRVEXMB004.vidanthealth.org> We are trying to create a consistent and efficient process for the triage of Radiology specimens going to Cytology, Surgical Pathology and Microbiology. I would really like to hear how other hospitals are handling this. Do you have a central drop off location and if so who handles this? Does radiology take them to the appropriate depts? Do you have the same process 8am-5pm and after hours or do you have different processes? I appreciate any feedback you are willing to give. Thanks, Dana ------------------------------------------------------------------------------ The contents of this e-mail (and any attachments) are confidential, may be privileged and may contain copyright material. You may only reproduce or distribute material if you are expressly authorized by us to do so. If you are not the intended recipient, any use, disclosure or copying of this email (and any attachments) is unauthorized. If you have received this e-mail in error, please notify the sender and immediately delete this e-mail and any copies of it from your system. ============================================================================== From LBUSTAMANTE at cvm.tamu.edu Tue Jun 23 12:27:38 2015 From: LBUSTAMANTE at cvm.tamu.edu (Bustamante, Lin) Date: Tue, 23 Jun 2015 17:27:38 +0000 Subject: [Histonet] pH measurements for tissue Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC901560A9F96@CVMMB02.cvm.tamu.edu> Does anyone knows how to measure pH in tissue? Any help will be greatly appreciated. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 From litepath2000 at yahoo.com Tue Jun 23 14:42:34 2015 From: litepath2000 at yahoo.com (NYSHisto) Date: Tue, 23 Jun 2015 19:42:34 +0000 (UTC) Subject: [Histonet] Beta 2 adrenergic receptor IHC Message-ID: <1272867775.5035502.1435088554567.JavaMail.yahoo@mail.yahoo.com> Does anyone have any recommendations for antibodies and or is aware of specific issues/limitations for Beta 2 adrenergic receptor IHC?? Working with murine WT & KO,? PFA fixed frozen sections.? Either IF or chromogenic are options...Open to any guidance or suggestions ?Thanks ? From KSimeone at leavittmgt.com Tue Jun 23 15:10:07 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Tue, 23 Jun 2015 20:10:07 +0000 Subject: [Histonet] FT NIGHT (3rd shift) POSITION DELRAY BCH FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C3DC9C2E2@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengimann at leavittmgt.com if interested. *full time position Mon-Fri or Sun-Thurs 6PM-2:30AM *MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo) *MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE! *VERY proficient in embedding and microtomy *must be self motivated, reliable and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) *some IHC experience preferred Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From jkiernan at uwo.ca Tue Jun 23 16:49:36 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Tue, 23 Jun 2015 16:49:36 -0500 Subject: [Histonet] Removing paraffin sections from glass slides In-Reply-To: <7300c6f216f8a.5589d45b@uwo.ca> References: <5C3E10119A1B824FBE92B08279F74A91025973BC@msgb10.nih.gov> <"028801d0ad9a$d63d8830$82b89890$@rowaihi"@alborglaboratories.com> <72e0847211858.5589d1b2@uwo.ca> <73c0b04c11db1.5589d1ee@uwo.ca> <73b0e9db175eb.5589d22d@uwo.ca> <7280e287165b6.5589d26b@uwo.ca> <7330d65a13045.5589d2a9@uwo.ca> <7300fad717933.5589d2e7@uwo.ca> <7300ddae12d90.5589d325@uwo.ca> <7290c74d13aec.5589d3a0@uwo.ca> <7340d9e9124ac.5589d3de@uwo.ca> <72d0d1ef172ca.5589d41d@uwo.ca> <7300c6f216f8a.5589d45b@uwo.ca> Message-ID: <736080e41588f.55898e20@uwo.ca> Why shouldn't the slide surfaces remain positively charged after removing the sections? Water, alcohol and xylene are all routinely used, and don't impair electrostatic forces that help to hold the sections on the slide. The positively charged surface is not a coating that can be washed or rubbed off. Joyce Weems's suggested method did not involve anything that might react chemically with the cationic (amino-hydrocarbon) groups that are covalently bound to the polysilicate structure of the glass. See http://publish.uwo.ca/~jkiernan/adhesivs.htm John Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada = = = On 23/06/15, Jamal wrote: > Dear Colleague > I just want to remind you.. > > After all of that procedure of removing the paraffin sections from the > positive charged slides. > The slides will not be positive charged anymore and not suitable for IHC. > Also the cost of the detergents and the chemicals which you used for > removing the tissue section is more costly than the slides. > > > > Best Regards, > > > Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg > Medical Laboratories | Mobile +966 503629832| > j.rowaihi at alborglaboratories.com > Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | > Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | > www.alborglaboratories.com > > > -----Original Message----- > From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] > Sent: Monday, June 22, 2015 5:53 PM > To: 'Coffey, Anna (NIH/NCI) [C]'; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Removing paraffin sections from glass slides > > This is what I would do... > > Soak the coverslip off in xylene > Either - rehydrate back to water and just wipe the tissue off - then dip > the slides in absolute and let dry.. > > OR - air dry out of xylene and wipe off with a wet gauze - then do the > alcohol dip > > Whichever worked best. Just my 2 cents.. > > Happy Monday!! > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems at emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's > Hospital and is intended for the sole use of the intended recipient(s). It > may contain information that is privileged and confidential. Any > unauthorized review, use, disclosure, or distribution is prohibited. If you > are not the intended recipient, please delete this message, and reply to the > sender regarding the error in a separate email. > > > -----Original Message----- > From: Coffey, Anna (NIH/NCI) [C] [mailto:anna.coffey at nih.gov] > Sent: Monday, June 22, 2015 10:25 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Removing paraffin sections from glass slides > > Hello, > > I'm wondering if anyone has experience removing dried unstained paraffin > sections from charged glass slides. I don't need to preserve the sections, > just want to reuse the slides. > > Thanks! > Anna > > Anna Coffey, MS, HTL(ASCP)CM > Histotechnologist > Center for Advanced Preclinical Research Frederick National Laboratory for > Cancer Research Leidos Biomedical Research, Inc. > Bld 539, 224 > Frederick, Maryland 21702 > anna.coffey at nih.gov > 301-846-1730 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From vollecra at gmail.com Tue Jun 23 17:00:30 2015 From: vollecra at gmail.com (Craig) Date: Tue, 23 Jun 2015 17:00:30 -0500 Subject: [Histonet] Antigen retrieval survey Message-ID: Hi, I am conducting a short 2 min survey for my science/business class examining current trends for antigen retrieval also known as heat induce epitope retrieval. Response will be greatly appreciated! https://www.surveymonkey.com/s/7989LKR Best, Craig Vollert Graduate Student Department of Pharmacological & Pharmaceutical Sciences SR2 521B College of Pharmacy University of Houston From simmca at UPMC.EDU Tue Jun 23 17:30:51 2015 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Tue, 23 Jun 2015 22:30:51 +0000 Subject: [Histonet] Removing paraffin sections from glass slides In-Reply-To: <736080e41588f.55898e20@uwo.ca> References: <5C3E10119A1B824FBE92B08279F74A91025973BC@msgb10.nih.gov> <"028801d0ad9a$d63d8830$82b89890$@rowaihi"@alborglaboratories.com> <72e0847211858.5589d1b2@uwo.ca> <73c0b04c11db1.5589d1ee@uwo.ca> <73b0e9db175eb.5589d22d@uwo.ca> <7280e287165b6.5589d26b@uwo.ca> <7330d65a13045.5589d2a9@uwo.ca> <7300fad717933.5589d2e7@uwo.ca> <7300ddae12d90.5589d325@uwo.ca> <7290c74d13aec.5589d3a0@uwo.ca> <7340d9e9124ac.5589d3de@uwo.ca> <72d0d1ef172ca.5589d41d@uwo.ca> <7300c6f216f8a.5589d45b@uwo.ca> <736080e41588f.55898e20@uwo.ca> Message-ID: Ventana loves that excuse for stain failures They even made us flip our slides upside down to place the control on the slide then flip them around to place the section on the slide due to the electrostatic forces Additionally all slides had to be air dried then placed in slide boxes prior to use Crap I say! Sent from my iPhone > On Jun 23, 2015, at 5:50 PM, John Kiernan wrote: > > Why shouldn't the slide surfaces remain positively charged after removing the sections? Water, alcohol and xylene are all routinely used, and don't impair electrostatic forces that help to hold the sections on the slide. The positively charged surface is not a coating that can be washed or rubbed off. > Joyce Weems's suggested method did not involve anything that might react chemically with the cationic (amino-hydrocarbon) groups that are covalently bound to the polysilicate structure of the glass. See http://publish.uwo.ca/~jkiernan/adhesivs.htm > > John Kiernan > Anatomy & Cell Biology > University of Western Ontario > London, Canada > = = = >> On 23/06/15, Jamal wrote: >> Dear Colleague >> I just want to remind you.. >> >> After all of that procedure of removing the paraffin sections from the >> positive charged slides. >> The slides will not be positive charged anymore and not suitable for IHC. >> Also the cost of the detergents and the chemicals which you used for >> removing the tissue section is more costly than the slides. >> >> >> >> Best Regards, >> >> >> Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg >> Medical Laboratories | Mobile +966 503629832| >> j.rowaihi at alborglaboratories.com >> Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | >> Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | >> www.alborglaboratories.com >> >> >> -----Original Message----- >> From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] >> Sent: Monday, June 22, 2015 5:53 PM >> To: 'Coffey, Anna (NIH/NCI) [C]'; histonet at lists.utsouthwestern.edu >> Subject: Re: [Histonet] Removing paraffin sections from glass slides >> >> This is what I would do... >> >> Soak the coverslip off in xylene >> Either - rehydrate back to water and just wipe the tissue off - then dip >> the slides in absolute and let dry.. >> >> OR - air dry out of xylene and wipe off with a wet gauze - then do the >> alcohol dip >> >> Whichever worked best. Just my 2 cents.. >> >> Happy Monday!! >> >> Joyce Weems >> Pathology Manager >> 678-843-7376 Phone >> 678-843-7831 Fax >> joyce.weems at emoryhealthcare.org >> >> >> >> www.saintjosephsatlanta.org >> 5665 Peachtree Dunwoody Road >> Atlanta, GA 30342 >> >> This e-mail, including any attachments is the property of Saint Joseph's >> Hospital and is intended for the sole use of the intended recipient(s). It >> may contain information that is privileged and confidential. Any >> unauthorized review, use, disclosure, or distribution is prohibited. If you >> are not the intended recipient, please delete this message, and reply to the >> sender regarding the error in a separate email. >> >> >> -----Original Message----- >> From: Coffey, Anna (NIH/NCI) [C] [mailto:anna.coffey at nih.gov] >> Sent: Monday, June 22, 2015 10:25 AM >> To: histonet at lists.utsouthwestern.edu >> Subject: [Histonet] Removing paraffin sections from glass slides >> >> Hello, >> >> I'm wondering if anyone has experience removing dried unstained paraffin >> sections from charged glass slides. I don't need to preserve the sections, >> just want to reuse the slides. >> >> Thanks! >> Anna >> >> Anna Coffey, MS, HTL(ASCP)CM >> Histotechnologist >> Center for Advanced Preclinical Research Frederick National Laboratory for >> Cancer Research Leidos Biomedical Research, Inc. >> Bld 539, 224 >> Frederick, Maryland 21702 >> anna.coffey at nih.gov >> 301-846-1730 >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ________________________________ >> >> This e-mail message (including any attachments) is for the sole use of >> the intended recipient(s) and may contain confidential and privileged >> information. If the reader of this message is not the intended >> recipient, you are hereby notified that any dissemination, distribution >> or copying of this message (including any attachments) is strictly >> prohibited. >> >> If you have received this message in error, please contact >> the sender by reply e-mail message and destroy all copies of the >> original message (including attachments). >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hlukey at msn.com Tue Jun 23 21:21:08 2015 From: hlukey at msn.com (Hugh Luk) Date: Tue, 23 Jun 2015 16:21:08 -1000 Subject: [Histonet] Removing paraffin sections from glass slides In-Reply-To: References: Message-ID: Hi Anna, You could deparaffinize/rehydrate to 10% (or so) bleach. Section(s) comes right off after ~1-60 minutes (or so). Did this by accident (that was a bad day). Deparaffinzation via Rene J Buesa's DWS (http://www.histosearch.com/ADP7HistologyWithoutXylene.pdf) method, or simply quick (20 seconds each) xylene/alcohol/water should suffice. I don't know what bleach will do to the charge on your plus slide, but I assume you should "Clean" with acid to be useful(?). I have been queried about reusing slides from several physicians in the past, but never been organized enough to try it. Good luck, in whatever you are doing. Hugh Hawaii > ---------------------------------------------------------------------- > Date: Mon, 22 Jun 2015 14:24:50 +0000 > From: "Coffey, Anna (NIH/NCI) [C]" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Removing paraffin sections from glass slides > Message-ID: <5C3E10119A1B824FBE92B08279F74A91025973BC at msgb10.nih.gov> > Content-Type: text/plain; charset="us-ascii" > > Hello, > > I'm wondering if anyone has experience removing dried unstained paraffin sections from charged glass slides. I don't need to preserve the sections, just want to reuse the slides. > > Thanks! > Anna > > Anna Coffey, MS, HTL(ASCP)CM > Histotechnologist > Center for Advanced Preclinical Research > Frederick National Laboratory for Cancer Research > Leidos Biomedical Research, Inc. > Bld 539, 224 > Frederick, Maryland 21702 > anna.coffey at nih.gov > 301-846-1730 > > > > ------------------------------ > > Message: 5 > Date: Mon, 22 Jun 2015 14:52:30 +0000 > From: "Weems, Joyce K." > To: "'Coffey, Anna (NIH/NCI) [C]'" , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Removing paraffin sections from glass slides > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > This is what I would do... > > Soak the coverslip off in xylene > Either - rehydrate back to water and just wipe the tissue off - then dip the slides in absolute and let dry.. > > OR - air dry out of xylene and wipe off with a wet gauze - then do the alcohol dip > > Whichever worked best. Just my 2 cents.. > > Happy Monday!! > > Joyce Weems > Pathology Manager > 678-843-7376 Phone > 678-843-7831 Fax > joyce.weems at emoryhealthcare.org > > > > www.saintjosephsatlanta.org > 5665 Peachtree Dunwoody Road > Atlanta, GA 30342 > > This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > > -----Original Message----- > From: Coffey, Anna (NIH/NCI) [C] [mailto:anna.coffey at nih.gov] > Sent: Monday, June 22, 2015 10:25 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Removing paraffin sections from glass slides > > Hello, > > I'm wondering if anyone has experience removing dried unstained paraffin sections from charged glass slides. I don't need to preserve the sections, just want to reuse the slides. > > Thanks! > Anna > > Anna Coffey, MS, HTL(ASCP)CM > Histotechnologist > Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. > Bld 539, 224 > Frederick, Maryland 21702 > anna.coffey at nih.gov > 301-846-1730 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 139, Issue 22 > ***************************************** From jkiernan at uwo.ca Tue Jun 23 23:46:25 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Tue, 23 Jun 2015 23:46:25 -0500 Subject: [Histonet] Antigen retrieval survey In-Reply-To: <7290934312c0e.558a361d@uwo.ca> References: <72d0b9d7158d2.558a2bbf@uwo.ca> <733099d113bdf.558a2bfc@uwo.ca> <72d0f01615b41.558a2cee@uwo.ca> <7340a2781450b.558a2d2d@uwo.ca> <73b0876c14a53.558a2d6b@uwo.ca> <72808aa917345.558a2de5@uwo.ca> <7280d4d313529.558a2e24@uwo.ca> <7340f81c17455.558a2e62@uwo.ca> <7340a35911ad5.558a2ea0@uwo.ca> <7360afcd15603.558a2edf@uwo.ca> <72e0ee3814fef.558a2f1d@uwo.ca> <7330a81813386.558a2f5b@uwo.ca> <7300bba61482b.558a2f99@uwo.ca> <7300b75e15ca0.558a2fd7@uwo.ca> <7300b8d211d63.558a3015@uwo.ca> <72e0bb3d1435d.558a3053@uwo.ca> <72e0aaa7112e9.558a3091@uwo.ca> <72908b9416703.558a310b@uwo.ca> <7300f54b15a23.558a3149@uwo.ca> <7300fb35128d3.558a3187@uwo.ca> <7330ecde15e88.558a31c5@uwo.ca> <7330c6ce13095.558a3240@uwo.ca> <72e0994516d22.558a327e@uwo.ca> <72808ef314bed.558a32bc@uwo.ca> <7340d61316c59.558a32fa@uwo.ca> <7360d02611040.558a3374@uwo.ca> <7360de3f128c0.558a33b3@uwo.ca> <72e0a5b513af7.558a342d@uwo.ca> <73c0df1c12c3c.558a346b@uwo.ca> <73b0865611a4c.558a34a9@uwo.ca> <73b0e85b100e1.558a3524@uwo.ca> <734082ed14169.558a3562@uwo.ca> <7340ea491763b.558a35a0@uwo.ca> <7340da8011f09.558a35df@uwo.ca> <7290934312c0e.558a361d@uwo.ca> Message-ID: <7290ddee11bfe.5589efd1@uwo.ca> Your 2 minutes would be better spent looking in an immunohistochemistry textbook. A small but excellent one is Polak, J.M. and Van Noorden, S. (1997). Introduction to Immunocytochemistry, 2nd ed. Royal Microscopical Society Microscopy Handbooks, 37. Oxford: BIOS Scientific Publications. You will find that there is an optimal technique of antigen retrieval for each antigen that has been critically studied. Some conditions (such as pH6, close to 100C for an hour) are OK for many antigens. Some require more alkaline solutions (eg pH9, more section losses!) and a few respond best to heating in a more acid (eg pH2) solution. With lower temperatures (eg 80C) longer times are generally needed. All sorts of chemicals have been included in antigen retrieval solutions, often without obvious reasons or explanations. There are published papers that compare retrieval conditions for antigens of importance in diagnostic pathology. Retrieval can sometimes be achieved without heating, as with proteolytic enzymes or 3M urea. With a survey you may find out which antigen retrieval methods are used by most of those who reply, but you will not learn anything about how to choose and use the methods, or why their discovery about 25 years ago was an important technological advance. Check out this classic paper with Web of Science, Scopus, or Google Scholar: Shi, S.-R., Key, M.E. and Kalra, K.L. (1991). Antigen retrieval in formalin-fixed, paraffin-embedded tissue: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. Journal of Histochemistry and Cytochemistry 39:741-748. The PDF can be downloaded for free. This paper has been cited by thousands of other publications. The titles of recent citing articles may help you find a good retrieval procedure for the antigen that you need to detect immunohistochemically. John Kiernan UWO, London, Canada = = = On 23/06/15, Craig wrote: > Hi, > > I am conducting a short 2 min survey for my science/business class > examining current trends for antigen retrieval also known as heat induce > epitope retrieval. Response will be greatly appreciated! > > https://www.surveymonkey.com/s/7989LKR > > Best, > Craig Vollert > Graduate Student > Department of Pharmacological & Pharmaceutical Sciences > SR2 521B > College of Pharmacy > University of Houston > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From j.benavides at eae.csic.es Wed Jun 24 05:33:47 2015 From: j.benavides at eae.csic.es (Julio Benavides) Date: Wed, 24 Jun 2015 12:33:47 +0200 Subject: [Histonet] pestivirus immunohistochemistry In-Reply-To: <7290ddee11bfe.5589efd1@uwo.ca> References: <72d0b9d7158d2.558a2bbf@uwo.ca> <733099d113bdf.558a2bfc@uwo.ca> <72d0f01615b41.558a2cee@uwo.ca> <7340a2781450b.558a2d2d@uwo.ca> <73b0876c14a53.558a2d6b@uwo.ca> <72808aa917345.558a2de5@uwo.ca> <7280d4d313529.558a2e24@uwo.ca> <7340f81c17455.558a2e62@uwo.ca> <7340a35911ad5.558a2ea0@uwo.ca> <7360afcd15603.558a2edf@uwo.ca> <72e0ee3814fef.558a2f1d@uwo.ca> <7330a81813386.558a2f5b@uwo.ca> <7300bba61482b.558a2f99@uwo.ca> <7300b75e15ca0.558a2fd7@uwo.ca> <7300b8d211d63.558a3015@uwo.ca> <72e0bb3d1435d.558a3053@uwo.ca> <72e0aaa7112e9.558a3091@uwo.ca> <72908b9416703.558a310b@uwo.ca> <7300f54b15a23.558a3149@uwo.ca> <7300fb35128d3.558a3187@uwo.ca> <7330ecde15e88.558a31c5@uwo.ca> <7330c6ce13095.558a3240@uwo.ca> <72e0994516d22.558a327e@uwo.ca> <72808ef314bed.558a32bc@uwo.ca> <7340d61316c59.558a32fa@uwo.ca> <7360d02611040.558a3374@uwo.ca> <7360de3f128c0.558a33b3@uwo.ca> <72e0a5b513af7.558a342d@uwo.ca> <73c0df1c12c3c.558a346b@uwo.ca> <73b0865611a4c.558a34a9@uwo.ca> <73b0e85b100e1.558a3524@uwo.ca> <734082ed14169.558a3562@uwo.ca> <7340ea491763b.558a35a0@uwo.ca> <7340da8011f09.558a35df@uwo.ca> <7290934312c0e.558a361d@uwo.ca> <7290ddee11bfe.5589efd1@uwo.ca> Message-ID: <20150624123347.Horde.SczPlWdYPYGJI8ZEHaT0Jw3@webmail.csic.es> Hi there, does any one use immunohistochemistry to detect pestivirus in formalin fixed tissues? I am thinking in veterinary diseases (Border disease and BVD). There use to be a very good antibody (clone 15c5) but it has become almost imposible to buy it. Any other clone that works (or a international source of 15c5)? Thansks a lot for you help! Cheers Julio From cjbulmer2526 at aol.com Wed Jun 24 07:26:15 2015 From: cjbulmer2526 at aol.com (Cindy Bulmer) Date: Wed, 24 Jun 2015 08:26:15 -0400 Subject: [Histonet] Thanks! Message-ID: <14e25886f8f-255f-16446@webprd-a32.mail.aol.com> To everyone who took the time to reply to my IHC survey, Big help!! Cindy Cindy Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX From aliciaroselange at gmail.com Wed Jun 24 11:44:10 2015 From: aliciaroselange at gmail.com (Alicia Lange) Date: Wed, 24 Jun 2015 09:44:10 -0700 Subject: [Histonet] Xylene Substitutes Message-ID: Hello Histo Land, Does anyone use xylene substitutes in their stain line only? I am especially interested in d-limonene options. Thanks in advance! From clmcmah at clemson.edu Wed Jun 24 13:28:44 2015 From: clmcmah at clemson.edu (Chad L McMahan) Date: Wed, 24 Jun 2015 18:28:44 +0000 Subject: [Histonet] Region III --- State of SC Call for HOD for NSH Message-ID: Hello Histonetters from SC, If you are interested in sitting for the House of Delegates (HOD) meeting in DC to represent SC please let me know. I must submit paperwork before July 4th. We have one slot to fill as delegate or alternate. Let me know if you are interested. Schedule for House of Delegates: Wednesday, September 2, 2015 - HOD Sign-in at 6:00 Wednesday, September 2, 2015 - HOD convenes at 7:00 Best regards, Chad M [http://www.tigersports.com/productimages/imageaddons11/ovalpawmagnet180.jpg] Chad L. McMahan, MBA, HT (ASCP)cm Clemson Bioengineering Biomaterials/Histology Lab Manager BioE Research Safety Liaison Clemson University, 104 Rhodes Hall, Clemson, SC 29634 CUBEInC, 200 Patewood Drive, Bldg C, Ste. 400, Greenville, 29615 864-656-5553 Clemson Office 864-284-6711 CUBEInC Office 864-656-4466 Fax 864-541-1340 Cell clmcmah at clemson.edu From rjbuesa at yahoo.com Wed Jun 24 14:38:49 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 24 Jun 2015 19:38:49 +0000 (UTC) Subject: [Histonet] Xylene Substitutes In-Reply-To: References: Message-ID: <1281494589.539134.1435174729503.JavaMail.yahoo@mail.yahoo.com> We used NOTHING in our staining line. We dewaxed with 2% dishwasher soap followed by the normal staining (no xylene ? no ethanol).After staining we dried the stained section in an oven at 60?C for 15 minutes ? coverslip (no ethanol ? no xylene).To process we used iso-propanol ? mineral oil ? paraffin wax no ethanol ? no xylene).Try it, you will "love it".Ren? On Wednesday, June 24, 2015 1:03 PM, Alicia Lange wrote: Hello Histo Land, Does anyone use xylene substitutes in their stain line only? I am especially interested in d-limonene options. Thanks in advance! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Thu Jun 25 09:39:07 2015 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 25 Jun 2015 10:39:07 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin 6/25/2015 Stay Cool and Beat the Heat this Summer!! Message-ID: <009301d0af54$b1e23510$15a69f30$@earthlink.net> Hi Histonetters! I hope you are enjoying your Summer!!! I have a quick question for you. **What are you doing to beat the heat??** I'm spending a lot of time in the AC or at the beach and drinking lots and lots of water!! As a Florida Girl here are some of my other strategies: **I carry a folding fan and a small cotton washcloth **Freeze about 2 inches of water in the bottom of the bottle and fill it right before you leave. **Freeze fruit and veggies in water and use them as ice cubes. I would love to hear what you are doing to stay cool this summer. So if you have a minute please shoot me back an email and let me know. Here in Orlando we have already had 42 days of 90 plus degree heat and today's only the 2nd day of summer. I can use all of the help I can get!!! The only thing hotter than the temps are my phone lines!! My phones are ringing off the hook with exciting opportunities. Please take a look at my current openings. All of these positions are full time permanent positions with excellent salaries and great benefits. Some of these clients offer generous SIGN ON BONUSES and most of them are RELIA EXCLUSIVES!! Here is the list of my current openings: HISTOLOGY MANAGEMENT: Histology Supervisor - Dallas, TX IHC Lead Tech - Harrisonburg, VA Lead Histology Tech - Central Coast, CA Lead Histology Tech - Atlanta, GA Histology Manager - East of Chicago HISTOLOGY TECHNICIAN/TECHNOLOGIST Histotechnician - Norfolk, VA 15K Sign On Bonus Histology Tech - Atlanta, GA Histotechnician - Nashville, TN IHC Tech - Harrisonburg, VA Histotechnician - Asheville, NC Histotech - Kingsport, TN Histotech - Hammond, IN If you or anyone you know might be interested in any of these opportunities please contact me ASAP. I can be reached at relia1 at earthlink.net or toll free at 866-607-3542. Refer a friend earn a bonus $$$ Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From akbitting at geisinger.edu Thu Jun 25 10:08:46 2015 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Thu, 25 Jun 2015 15:08:46 +0000 Subject: [Histonet] IHC repeat rates Message-ID: <57b16d58c10341da9ed70a98d0aa6d13@GHSEXMBX4W12V.geisinger.edu> What does this group feel is an acceptable repeat rate for automated IHC staining?? Thanks for your responses in advance. Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From tbraud at holyredeemer.com Thu Jun 25 14:32:11 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 25 Jun 2015 19:32:11 +0000 Subject: [Histonet] IHC repeat rate In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F801CB32@HRHEX02-HOS.holyredeemer.local> I once had a vendor tell me that 5% was an acceptable rate. I am more inclined to use 2% Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 4. IHC repeat rates (Bitting, Angela K.) Message: 4 Date: Thu, 25 Jun 2015 15:08:46 +0000 From: "Bitting, Angela K." Subject: [Histonet] IHC repeat rates What does this group feel is an acceptable repeat rate for automated IHC staining?? Thanks for your responses in advance. Angie *************************************** From gayle.callis at bresnan.net Thu Jun 25 14:33:16 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Thu, 25 Jun 2015 13:33:16 -0600 Subject: [Histonet] Xylene substitutes in staining line Message-ID: <000a01d0af7d$c8fcae00$5af60a00$@bresnan.net> You wrote: Hello Histo Land, Does anyone use xylene substitutes in their stain line only? I am especially interested in d-limonene options. Thanks in advance! **************************************************************************** ***************************************** We used xylene substitutes in the stain line for both deparaffinization and dehydration but did not use limonene (citrus smelling). We preferred the Clearite 3 (Richard Allan) i.e. single aliphatic hydrocarbons also available from other vendors, or Propar (Anatech) for this purpose. Limonene was particularly offensive and personally made me turn green with nausea! It was banned from my lab due to intolerance to the smell which also included banning limonene based household cleaners in my home. I think people can become sensitized to this substitute so be careful about handling and working under a hood. We also used Clearite 3 for tissue processing to eliminate xylene. There are caveats about using xylene substitutes. They are sensitive to residual water carry over and do not clear water as well as xylene. We added an extra stations to deparaffinize sections and at the end of dehydration sequence ensure NO residual water carry over before mounting a cover glass. On some high humidity days, Clearite 3 would be cloudy meaning the last stations with Clearite had to be changed to fresh solvent not exposed to humid air. To combat a water carryover problem commonly not seen with xylene and its ability to handle water carryover better, we rotated the substitutes frequently during a work week so the last station was fresh. If you have cloudy looking sections after cover slipping from a xylene substitute, then you have water carry over. A hint is if the last alcohol in the dehydration series is pink, then there is water carryover. Due diligence is necessary to avoid poor paraffin removal and also good dehydration. In a very busy laboratory, this means extra time and expense to deal with a xylene substitute. The test for paraffin carry over in deparaffinization sequence, one can pipette a few mls of last 95% alcohol (closest to water) into a glass beaker of tap water. It the aliquot turns the water cloudy, then you have paraffin carry over into the alcohol. If not seen in last 95%, the test the 95% station by working backwards in the deparaffinizing sequence. If any alcohol test is cloudy, then that alcohol and all previous stations were changed in our lab. Some rotation of clear test was done into an earlier station slot. This will test also works when using xylene. However, I really like the emerging practice of using hot soapy water for deparaffinizing sections recently discussed in depth on Histonet. There is a wonderful publication by Tony Henwood et al, J of Histotechnol 2013; 36(2):45-50. Tresa Goins also entered in on this discussion about their lab's success with this method. Go to Histonet Archives and read the commentary. It certainly means less dependence on organic solvents, less exposure to potentially toxic chemicals, and the expense of solvent disposal. I think the soap method is definitely worth trying. Hope this helps Gayle M. Callis HTL/HT/MT(ASCP) From lblazek at digestivespecialists.com Thu Jun 25 15:22:54 2015 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Thu, 25 Jun 2015 16:22:54 -0400 Subject: [Histonet] IHC repeat rate In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F801CB32@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F801CB32@HRHEX02-HOS.holyredeemer.local> Message-ID: <5A2BD13465E061429D6455C8D6B40E3917427EBF81@IBMB7Exchange.digestivespecialists.com> I would think that it would really depend on if it is a repeat due to an instrument problem or a human error. I think that if a vendor says that a 5% repeat rate is acceptable for their instrument I would look for another vendor. -----Original Message----- From: Terri Braud [mailto:tbraud at holyredeemer.com] Sent: Thursday, June 25, 2015 3:32 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC repeat rate I once had a vendor tell me that 5% was an acceptable rate. I am more inclined to use 2% Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 4. IHC repeat rates (Bitting, Angela K.) Message: 4 Date: Thu, 25 Jun 2015 15:08:46 +0000 From: "Bitting, Angela K." Subject: [Histonet] IHC repeat rates What does this group feel is an acceptable repeat rate for automated IHC staining?? Thanks for your responses in advance. Angie *************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Thu Jun 25 19:15:40 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 26 Jun 2015 00:15:40 +0000 Subject: [Histonet] IHC repeat rates In-Reply-To: <57b16d58c10341da9ed70a98d0aa6d13@GHSEXMBX4W12V.geisinger.edu> References: <57b16d58c10341da9ed70a98d0aa6d13@GHSEXMBX4W12V.geisinger.edu> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53F9F14@xmdb04.nch.kids> The RCPA (in Australia) recently requested that laboratories perform Technical Failure Repeat Audits that might be of use. Subsequently I produced a brief report for our Society's journal: Audit of Technical Failures Tony Henwood Histopathology The Children's Hospital at Westmead, Sydney The RCPA has requested that laboratories implement the Internal Quality Assurance Framework-Pilot Program for the interpretative and morphological disciplines. There is also a requirement for 2.5 hours of 'other', internal quality activities relating to the technical and service measures section of the framework (over a 3 month timeframe). One of these is the re-staining required due to technical failure (H&E, special stain, Immunoperoxidase, etc.). This Technical Failures Audit records the number of stains of a certain type requiring repeat over total number stained during a 3 month audit period. One would also include those instances where recuts or deeper sections are requested due to sectioning artefacts or incomplete full face. The necessity for repeat staining reflects the quality of technical work and has impact on overall laboratory efficiency. This audit is one of the measures recommended to monitor the analytic phase of the test cycle. The analytic phase of the test cycle represents all of the steps in processing and evaluating the specimen in the laboratory until a diagnosis is rendered (3). Other audits include (6): * Quality of histologic sections * Specimens lost in processing * Turnaround time (TAT) * Block labelling * Slide labelling * Extraneous tissue * TAT for immunohistochemical analysis * Report audit for integration of stains with morphologic diagnosis * Annual review of antibody inventory and frequency of use The Association of Directors of Anatomic and Surgical Pathology have included "Frequency and causes of repeated stains" under Immunohistochemical analysis (6). But one would think that this could, and probably should, be applied across all the technical aspects of the analytical phase of histopathology. An effective audit needs to be relevant, objective, quantified and repeatable with formal identification of areas requiring improvement (1, 2). It also provides a method for reassessment of performance once appropriate changes have been implemented (1). Although errors causing patient harm are unfortunate, it must be acknowledged that a certain error rate is prevalent. Error levels that may be deemed within an acceptable range should be determined based on the literature for that measure, with the goal of modification by continuous improvement (6) It has been recommended that a Quality Assurance Monitor should include a targeted acceptable range and reference benchmark data (3). It is appropriate to complete this audit, but it can be of limited use unless we have appropriate benchmarks to compare our laboratories data to. >From the literature: * Zuk et al (1) found 19% of cases were technical unsatisfactory ("holes", folds, debris, "chatters", "scores" or absence of full transverse section). They also found that H&E staining was poor in a 2% of cases. This study recorded all cases of technical failure, not just those requiring a repeat in order to facilitate a diagnosis. * In the manufacturing industry, six sigma has emerged as a generic quality standard. This standard aims to have the total number of failures in quality, or customer satisfaction at 3.4 defects or fewer than 4 defects per million products (4). * Morelli et al (5) found in their study of errors in histology preparation that 0.03% of cases required the repeat of one or more processes; To implement this audit we also need to determine the "Rules of Engagement"- what parameters need to be observed in order to render the results meaningful? * Should a repeat caused by microscopic incomplete full face (not obvious from scrutinising the paraffin block) be counted? * Should further sections and in particular serials to improve the detection of a small focus of disease be counted as a technical failure? So, what do you think? Please send your thoughts to the editor References: 1. Zuk, J. A., Kenyon, W. E., & Myskow, M. W. (1991). Audit in histopathology: description of an internal quality assessment scheme with analysis of preliminary results. Journal of clinical pathology, 44(1), 10-16. 2. Shaw CD, Costain DW. "1989" Guidelines for medical audit: seven principles. Br Med J 299:498-9. 3. Nakhleh, R. E. (2009). Core components of a comprehensive quality assurance program in anatomic pathology. Advances in anatomic pathology, 16(6), 418-423. 4. Nakhleh, R. E. (2006). What is quality in surgical pathology?. Journal of clinical pathology, 59(7), 669-672. 5. Morelli, P., Porazzi, E., Ruspini, M., Restelli, U., & Banfi, G. (2013). Analysis of errors in histology by root cause analysis: a pilot study. J Prev Med Hyg, 54, 90-6. 6. Association of Directors of Anatomic and Surgical Pathology. (2006). Recommendations for quality assurance and improvement in surgical and autopsy pathology. Am J Clin Pathol 126:337-340 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Bitting, Angela K. [mailto:akbitting at geisinger.edu] Sent: Friday, 26 June 2015 1:09 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] IHC repeat rates What does this group feel is an acceptable repeat rate for automated IHC staining?? Thanks for your responses in advance. Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From Timothy.Morken at ucsf.edu Fri Jun 26 16:51:41 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 26 Jun 2015 21:51:41 +0000 Subject: [Histonet] Leica Cerebro Message-ID: <761E2B5697F795489C8710BCC72141FF3684367B@ex07.net.ucsf.edu> Pam, we considered Cerebro a couple years ago but it was not really ready for prime time yet. It did not have the capability to link with our Cerner Copath Plus at that time. However, we did go see it live in some stand-alone labs and it looked very nice and well thought out The two major issue we ran up against, with Cerebro and Ventana Vantage, is that 1) Copath requires installation of their Advanced Barcoding and Tracking system (AB&T) in order to communicate with ANY third party system, which means you would have two full-featured barcoding systems installed. That does not make financial sense. We originally considered other systems besides AB&T because at the time we evaluated it several years ago it was very rudimentary and not nearly as full featured as Cerebro or Vantage. But before we made a final decision AB&T had been brought up to pretty much the same level in functionality. Secondly, Copath AB&T, when used as middleware for another system, will not allow bi-directional communication, except for status updates (ie, when a block is scanned and it's staus changes from Embedded to Cut, the third party system can send that message to Copath for registration). What is NOT allowed is to enter blocks, stains, etc into the third party system and have that information written back to the Copath database. Therefore, any additions must be made in Copath. That limits a third party system to tracking only. Because of those two major constraints we installed AB&T alone. It works very well and we are happy with it. The only downside is that Copath has very poor statistical reporting capabilities so you have to either download report data to Excel or some other program and do analysis there, or install a third party system to build reports off the Copath database. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center [Histonet] Leica cerebo Pam DeFazio Thu, 18 Jun 2015 04:53:58 -0700 Anyone using this? We use cerner copath. Thoughts? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Fri Jun 26 17:10:32 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 26 Jun 2015 22:10:32 +0000 Subject: [Histonet] IHC turnaround time Message-ID: <761E2B5697F795489C8710BCC72141FF368436B6@ex07.net.ucsf.edu> Dawn, I'd say 12-5 is a good TAT for any lab. Same day TAT is excellent. Maybe they don't want to stay late to ready the slides? We do 250 - 300/day, 5-6,000/month but we have extended working hours. We cut recuts from 11pm thru to 3pm and start loading the Bond and Ventana at 4am. Then they get loaded continuously until about 4pm. We'll do overnight if necessary. This and linking our stainers to our LIS through barcodes greatly reduced TAT. Just the linkage saved us a couple working hours per day in data entry into the stainers - now it is all entered automatically by the system. When the stainer scans the slide it links to the order and fills in all the info. At least for the Bond. Ventana is not as clean. The order information is sent to the stainer, but because they insist on using their own barcode so it takes double labeling for their system. But we do 85% on the Bonds Our TAT went from up to 36 hours down to about 5 hours average. We get some done in 3 hours just due to the stain being ordered and us picking it up and cutting right away. The pathologists don't complain at all anymore. In fact they were kind of incredulous when they would order in the late morning and get it in the afternoon, or order in the late afternoon and it would be in their mailbox when they came in the next morning. BTW, for the direct work on the stainer it takes only 2 FTE (two shifts). Counting the recuts people it is another 1.5 FTE, and counting the distribution person it is one more FTE. So about 4.5 total FTE for cutting, staining, and slide distribution. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center Histonet] IHC turnaround time Olszewski, Dawn Fri, 19 Jun 2015 08:11:44 -0700 Hi Histonetters, 6-19-15 We are wondering what the average turnaround times for IHC are for other labs. We use the Biocare Intellipath instrumentation. It holds up to 50 slides ( 10 slides per 5 racks) with continuous feed per open rack. All IHC orders placed by 12pm are usually out the same day by 5pm. We average 434 IHC slides per month and have a staff of 3 FTE's. A pathologist has voiced concerns over our IHC output. We are trying to determine "best practices" for IHC turnaround time as measured from time order placed to time of slide delivery. If you could respond with your IHC TAT including number of techs and average of IHC slides monthly, we would appreciate any input you may be able to provide. Thank you in advance, Dawn Olszewski, HTL(ASCP)QIHC From Timothy.Morken at ucsf.edu Fri Jun 26 17:44:56 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 26 Jun 2015 22:44:56 +0000 Subject: [Histonet] Lab furniture Message-ID: <761E2B5697F795489C8710BCC72141FF36843770@ex07.net.ucsf.edu> Jill, I ordered custom made tables from OnePointe solutions. Pricing is very reasonable http://lab.onepointesolutions.com/custom-lab-tables/ [Histonet] LAB furniture Jill Cox Thu, 18 Jun 2015 09:12:26 -0700 Hi, I am in the process of adding lab furniture to the lab and was wondering if anyone has a good company that will make workbenches. I am adding an island trying to get 4' x 12'. Someone local to the Phoenix area would be great. Thank you in advance! Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From erinp517 at aol.com Sun Jun 28 16:26:35 2015 From: erinp517 at aol.com (Erin) Date: Sun, 28 Jun 2015 17:26:35 -0400 Subject: [Histonet] H&E staining issues Message-ID: <6577C56E-4983-46D5-A0BD-16DFF0610120@aol.com> Was wondering if anyone else is experiencing any h&e staining issues recently using the symphony stainer? Our lab has been experiencing uneven staining and dark mucin staining especially on our Gi biopsies. This has been happening on specimens that have been processed on different processors, two different symphony stainers that are located in two different buildings and has just recently become a real issue within the past two months. I'm thinking this is reagent issue and was wondering if anyone else is having the same staining problem recently? Sent from my iPad From THicks at gulfcoastdermpath.com Sun Jun 28 17:41:54 2015 From: THicks at gulfcoastdermpath.com (Trish Hicks) Date: Sun, 28 Jun 2015 22:41:54 +0000 Subject: [Histonet] Dermatopathogist Workload Message-ID: Does anyone have a standard or an average for number of slides or cases a dermatopathologist can read in a day not including IHC or special stains? Thank you, Trish Hicks BS, HTL (ASCP) Gulf Coast Dermatopathology (813) 882-4206 CONFIDENTIALITY NOTICE: The contents of this email message and any attachments are intended solely for the addressee(s) and may contain confidential and/or privileged information and may be legally protected from disclosure. If you are not the intended recipient of this message or their agent, or if this message has been addressed to you in error, please immediately alert the sender by reply email and then delete this message and any attachments. If you are not the intended recipient, you are hereby notified that any use, dissemination, copying, or storage of this message or its attachments is strictly prohibited. From kstoll at mcw.edu Mon Jun 29 09:14:33 2015 From: kstoll at mcw.edu (Stoll, Kathryn) Date: Mon, 29 Jun 2015 14:14:33 +0000 Subject: [Histonet] Brain Tissue Processing Message-ID: Hello, I am looking for help with tissue processing of brain. The pieces are large, they fill a super cassette. Does anyone have a protocol they could share. Thanks in advance. Kathryn Stoll Supervisor Histology Clinical and Translational Research Core Lab Medical College of Wisconsin 9200 W. Wisconsin Ave Room 1176 Milwaukee WI 53226 Phone: 414-805-1525 Fax: 414-805-1528 From simmca at UPMC.EDU Mon Jun 29 09:44:13 2015 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Mon, 29 Jun 2015 14:44:13 +0000 Subject: [Histonet] Brain Tissue Processing In-Reply-To: References: Message-ID: <6295CCF61B0DBB4C9248F51F8940E6A1011EF675@MSXMBXNSPRD09.acct.upmchs.net> Autopsy Program 13h 36m Step Reagent Time Temp. Vac Stir Drip Time 1 Fixative 30 37 Cycle Medium 10 2 Fixative 30 37 Cycle Medium 10 3 Dehydrant 60 37 Cycle Medium 10 4 Dehydrant 60 37 Cycle Medium 10 5 Dehydrant 60 37 Cycle Medium 10 6 Dehydrant 60 37 Cycle Medium 10 7 Dehydrant 90 37 Cycle Medium 10 8 Dehydrant 90 37 Cycle Medium 10 9 Xylene 45 37 Cycle Medium 10 10 Xylene 60 37 Cycle Medium 10 11 Xylene 60 37 Cycle Medium 10 12 Wax 40 60 Cycle Medium 10 13 Wax 60 60 Cycle Medium 10 14 Wax 60 60 Cycle Medium 30 Works PERFECTLY every time! Chris Simmons B.S., A.S., HTL(ASCP) Supervisor, UPP Dermatopathology 412.864.3880 office 412.612.0881 cell -----Original Message----- From: Stoll, Kathryn [mailto:kstoll at mcw.edu] Sent: Monday, June 29, 2015 10:15 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Brain Tissue Processing Hello, I am looking for help with tissue processing of brain. The pieces are large, they fill a super cassette. Does anyone have a protocol they could share. Thanks in advance. Kathryn Stoll Supervisor Histology Clinical and Translational Research Core Lab Medical College of Wisconsin 9200 W. Wisconsin Ave Room 1176 Milwaukee WI 53226 Phone: 414-805-1525 Fax: 414-805-1528 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt at pa-ucl.com Mon Jun 29 10:46:45 2015 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Mon, 29 Jun 2015 15:46:45 +0000 Subject: [Histonet] fat pad smears for amyloid Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C3601159FCA22@PEITHA.wad.pa-ucl.com> Good Morning! How are you pretreating fat pad smears for amyloid? Fixing in 95% and then air drying before staining? We are running them on an Artisan stainer. Thanks for your input- Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From smmiller at comhs.org Mon Jun 29 11:32:50 2015 From: smmiller at comhs.org (Sheila Miller) Date: Mon, 29 Jun 2015 16:32:50 +0000 Subject: [Histonet] Leica Cerebro In-Reply-To: <761E2B5697F795489C8710BCC72141FF3684367B@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF3684367B@ex07.net.ucsf.edu> Message-ID: <625ce96350a64866a223f73b162fef10@CHVSEXCASMB02.comhs.org> Curious to know if any Softpath users are using Leica Cerebro? -Sheila -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Friday, June 26, 2015 4:52 PM To: Histonet Subject: [Histonet] Leica Cerebro Pam, we considered Cerebro a couple years ago but it was not really ready for prime time yet. It did not have the capability to link with our Cerner Copath Plus at that time. However, we did go see it live in some stand-alone labs and it looked very nice and well thought out The two major issue we ran up against, with Cerebro and Ventana Vantage, is that 1) Copath requires installation of their Advanced Barcoding and Tracking system (AB&T) in order to communicate with ANY third party system, which means you would have two full-featured barcoding systems installed. That does not make financial sense. We originally considered other systems besides AB&T because at the time we evaluated it several years ago it was very rudimentary and not nearly as full featured as Cerebro or Vantage. But before we made a final decision AB&T had been brought up to pretty much the same level in functionality. Secondly, Copath AB&T, when used as middleware for another system, will not allow bi-directional communication, except for status updates (ie, when a block is scanned and it's staus changes from Embedded to Cut, the third party system can send that message to Copath for registration). What is NOT allowed is to enter blocks, stains, etc into the third party system and have that information written back to the Copath database. Therefore, any additions must be made in Copath. That limits a third party system to tracking only. Because of those two major constraints we installed AB&T alone. It works very well and we are happy with it. The only downside is that Copath has very poor statistical reporting capabilities so you have to either download report data to Excel or some other program and do analysis there, or install a third party system to build reports off the Copath database. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center [Histonet] Leica cerebo Pam DeFazio Thu, 18 Jun 2015 04:53:58 -0700 Anyone using this? We use cerner copath. Thoughts? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and attachment(s), if any, is intended for the sole use of the individual and/or entity of which it is addressed, and may contain information that is privileged,confidential and prohibited from disclosure under applicable law. If you are not the addressee, or authorized to receive this on behalf of the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone this message or any part thereof. If you have received this in error, please immediately advise the sender by e-mail and delete this information and all attachments from your computer and network. Thank you. From KSimeone at leavittmgt.com Mon Jun 29 11:57:21 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Mon, 29 Jun 2015 16:57:21 +0000 Subject: [Histonet] FT NIGHT (3rd shift) POSITION DELRAY BCH FL In-Reply-To: <43944B1DBAAC2846B7B9D626B5F1233C3DC9C2E2@vm-email.leavittmgt.com> References: <43944B1DBAAC2846B7B9D626B5F1233C3DC9C2E2@vm-email.leavittmgt.com> Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C3E637EC3@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengimann at leavittmgt.com if interested. *full time position Mon-Fri or Sun-Thurs 6PM-2:30AM *MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo half of your shift) *MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE! *VERY proficient in embedding and microtomy *must be self motivated, reliable and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) *some IHC experience preferred Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From aeck at dh.org Mon Jun 29 12:12:16 2015 From: aeck at dh.org (Eck, Allison) Date: Mon, 29 Jun 2015 17:12:16 +0000 Subject: [Histonet] billing question Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A9718DA42@DH-MAIL01.dhorg.org> I am looking for advice on a current billing problem. Breast specimens: We recieve the lumpectomy with orienting sutures - 88307 along with that are the accompanying margins (inferior, superior, medial, etc). Our breast surgeon has been placing orienting sutures on all of her margins. We had previously been billing under the assumption that the unsutured margins were 88305, but now with the sutures should we be billing an 88307 or still 88305 since they are margins? Thank you in advance for your help Allison From patpxs at gmail.com Mon Jun 29 12:33:37 2015 From: patpxs at gmail.com (Paula Sicurello) Date: Mon, 29 Jun 2015 10:33:37 -0700 Subject: [Histonet] CAP Question Message-ID: Good Morning Netters, How many antibodies are y'all testing to meet the CAP guideline (COM.04250) that requires comparison testing of equipment performing the same test (autostainers, for instance) twice a year? I read it as requiring all antibodies are tested on all platforms, but that seems a bit much. Enquiring minds want to know. Thanks a bunch! Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UCSD Medical Center 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From mjones at metropath.com Mon Jun 29 12:51:05 2015 From: mjones at metropath.com (Michael Ann Jones) Date: Mon, 29 Jun 2015 17:51:05 +0000 Subject: [Histonet] CAP Question In-Reply-To: References: Message-ID: It does seem like a bit much, but that?s what we did All antibodies, we ran, exact same lot # ran 3 on one machine and 3 on another. Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com On 6/29/15, 11:33 AM, "Paula Sicurello" wrote: >Good Morning Netters, > >How many antibodies are y'all testing to meet the CAP guideline >(COM.04250) that requires comparison testing of equipment performing the >same test (autostainers, for instance) twice a year? > >I read it as requiring all antibodies are tested on all platforms, but >that >seems a bit much. > >Enquiring minds want to know. > >Thanks a bunch! > >Paula > > > > >Paula Sicurello, HTL (ASCP)CM > >Histotechnology Specialist > >UCSD Medical Center > >200 Arbor Drive > >San Diego, CA 92103 > >(P): 619-543-2872 > > > >*Confidentiality Notice*: The information transmitted in this e-mail is >intended only for the person or entity to which it is addressed and may >contain confidential and/or privileged material. Any review, >retransmission, dissemination or other use of or taking of any action in >reliance upon this information by persons or entities other than the >intended recipient is prohibited. If you received this e-mail in error, >please contact the sender and delete the material from any computer. >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems at emoryhealthcare.org Mon Jun 29 13:12:12 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Mon, 29 Jun 2015 18:12:12 +0000 Subject: [Histonet] billing question In-Reply-To: <4ED8C96A8F20FC4F883A92E2A0A0D64A9718DA42@DH-MAIL01.dhorg.org> References: <4ED8C96A8F20FC4F883A92E2A0A0D64A9718DA42@DH-MAIL01.dhorg.org> Message-ID: Breast for margins is an 88307. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Eck, Allison [mailto:aeck at dh.org] Sent: Monday, June 29, 2015 1:12 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] billing question I am looking for advice on a current billing problem. Breast specimens: We recieve the lumpectomy with orienting sutures - 88307 along with that are the accompanying margins (inferior, superior, medial, etc). Our breast surgeon has been placing orienting sutures on all of her margins. We had previously been billing under the assumption that the unsutured margins were 88305, but now with the sutures should we be billing an 88307 or still 88305 since they are margins? Thank you in advance for your help Allison _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From akemiat3377 at gmail.com Mon Jun 29 20:04:58 2015 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Mon, 29 Jun 2015 18:04:58 -0700 Subject: [Histonet] GI Pathology Req's Message-ID: Hello Histolandl! I would like to reach out to all of you who do GI pathology. Our facility is going through a computer systems change for our endoscopy center, as well as the upstairs doctors offices, billing department, and the AP lab. I was wondering if any of you would care to share your GI requisition form. We would like to incorporate a new version for our endoscopy center. I would greatly appreciate it. Please send to my email address. Thank you in advance for your assistance! Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com Tele: (831) 375-3577 X117 From akemiat3377 at gmail.com Mon Jun 29 21:30:52 2015 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Mon, 29 Jun 2015 19:30:52 -0700 Subject: [Histonet] GI Pathology Req's In-Reply-To: <7096AA66-498B-4BD7-9EBE-218772CF76E3@gmail.com> References: <7096AA66-498B-4BD7-9EBE-218772CF76E3@gmail.com> Message-ID: <3C8326AF-9B11-47AF-9622-BC679E2C1628@gmail.com> Just an FYI- We do not have a LIS System! The GI CEO and administrator DOES NOT want to invest in a LIS System! Our pathologists are contracted, so they just go with the flow? which is difficult for me to have support to upgrade our system. As usual, we are an after thought! When I came here 4 years ago, I thought I was in a time machine and had been transported back to 1989 before they had lab computers or LIS Systems! We are currently using ?PCM?. It is geared towards GI MD?s and their practice. It is a system which is used for scheduling and all patient information. It interfaces with the billing system. This system was in place before I got here. Currently, my assistant has to create a Path Report and type in gross, etc? then the pathologist types in his microscopic findings and sign?s the report. Everything is hard copy?.. I have basically time warped to before the 90?s when I created all these workload recording forms up. We have hard copy req forms which are hand written by the nurses in endoscopy with all the patient and specimen information. We do have an automated cassette printer, but we hand write slides. We hand write an embedding log with # of number of pieces in the cassette, etc?for QC purposes. I hand write CPT charges on a hard copy and submit to the billing dept. Nothing is bar coded, etc?. It is better now that I have an assistant. The endoscopy center has upgraded to ?Provation?. It interfaces with our current ?PCM? (Prime Clinical Management System) which is antiquated! The new computer system for scheduling and billing is "Athena Healthcare? it is geared for billing and appointment scheduling etc.... We are also interfaced with Elkay? and are in training now and go live August 11th! We are still going to have to do the same procedure with pathology as usual, but we have to create the templates again! They are not getting us a LIS system to interface! Oh wait, I?ve been a histologist for 50 years and still going strong! It?s frustrating, but at least I live in a beautiful area! Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com Tele: (831) 375-3577 X117 Cell: (408) 335-9994 > On Jun 29, 2015, at 7:05 PM, Cristi Rigazio wrote: > > I am not at work right now but we have some great ideas. What EMR and LIS are you using? > > Sent from my iPhone > >> On Jun 29, 2015, at 6:04 PM, Eileen Akemi Allison wrote: >> >> Hello Histolandl! >> >> I would like to reach out to all of you who do GI pathology. Our facility is going through a computer systems change for our endoscopy center, as well as the upstairs doctors offices, billing department, and the AP lab. I was wondering if any of you would care to share your GI requisition form. We would like to incorporate a new version for our endoscopy center. I would greatly appreciate it. Please send to my email address. >> >> Thank you in advance for your assistance! >> >> Akemi Allison BS, HT/HTL (ASCP) >> Pathology Manager >> Monterey Bay GI Consultants Laboratory >> 23 Upper Ragsdale Drive, Suite 200 >> Monterey, CA 93940 >> W: Email: aallison at montereygi.com >> H: Email: akemiat3377 at gmail.com >> Tele: (831) 375-3577 X117 >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From maxim_71 at mail.ru Mon Jun 29 22:42:17 2015 From: maxim_71 at mail.ru (=?UTF-8?B?0J/QtdGI0LrQvtCyINCc0LDQutGB0LjQvA==?=) Date: Tue, 30 Jun 2015 06:42:17 +0300 Subject: [Histonet] =?utf-8?q?CAP_checklikst_for_pathology_lab?= Message-ID: <1435635737.466939592@f366.i.mail.ru> Dear histonetters, can you send me, pleasse, the last pdf version cap checklist for pathology lab? Or reference where I can download it. Sincerely, Maxim Peshkov, Russia, Taganrog. From Bonnie.Whitaker at osumc.edu Tue Jun 30 05:22:19 2015 From: Bonnie.Whitaker at osumc.edu (Whitaker, Bonnie) Date: Tue, 30 Jun 2015 06:22:19 -0400 Subject: [Histonet] CAP checklikst for pathology lab In-Reply-To: <1435635737.466939592@f366.i.mail.ru> References: <1435635737.466939592@f366.i.mail.ru> Message-ID: <6B106EE8C8AAEF449DEA97921DEC11670E18DD4A66@EXMBOX-VP05.OSUMC.EDU> The checklists are now customized, and your version must be downloaded from CAP.org. Good luck! Bonnie Sent with Good (www.good.com) -----Original Message----- From: ?????? ?????? [maxim_71 at mail.ru] Sent: Monday, June 29, 2015 11:43 PM Eastern Standard Time To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CAP checklikst for pathology lab Dear histonetters, can you send me, pleasse, the last pdf version cap checklist for pathology lab? Or reference where I can download it. Sincerely, Maxim Peshkov, Russia, Taganrog. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQICAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=tCmD3XGrX8z8GcLF2_0n5BVJIY0w9vMg5zYQrHXdnzQ&s=PfUmcwj_GLtjW5fDn1Y2CqAfR62z1lcoFYFyz3NWZrY&e= From bliven.laura at marshfieldclinic.org Tue Jun 30 09:14:50 2015 From: bliven.laura at marshfieldclinic.org (Bliven, Laura M) Date: Tue, 30 Jun 2015 14:14:50 +0000 Subject: [Histonet] PDL2 by Immunohistochemistry Message-ID: Looking for a lab to perform PDL2 (not PDL1). Any ideas? Thanks, Laura Bliven.laura at marshfieldclinic.org ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From christina.wolfe at bms.com Tue Jun 30 10:15:13 2015 From: christina.wolfe at bms.com (Wolfe, Christina) Date: Tue, 30 Jun 2015 15:15:13 +0000 Subject: [Histonet] Suggestions for staining ground substances in the Heart Message-ID: <6014eb4d5aad426baf62e23118b93963@BN1PR26MB0001.067d.mgd.msft.net> Hi all, We are interested in staining ground substances in the heart. Are there other stains that will work beside the Pentachrome? We have tried the Movat's pentachrome (commercial kit) and are able to demonstrate ground substance in the bone with the alcian blue part of this stain. In our hands the goblet cells of the gut and the ground substance in the heart are devoid of staining. We have tried sections cut at 4 and 6 microns. Any thoughts/suggestions? Kristie Christina Wolfe, BSHA, MLT (ASCP), HT, QIHC Drug Safety Evaluation/Bristol-Myers Squibb Pathology Dept. 812-307-2093 ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. From tbraud at holyredeemer.com Tue Jun 30 13:31:04 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 30 Jun 2015 18:31:04 +0000 Subject: [Histonet] Breast billing question In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F801DC37@HRHEX02-HOS.holyredeemer.local> We bill the lumpectomy specimen (as long as it contains tumor) as an 88307. Additional margins submitted in separate containers are billed each as 88305, breast biopsy. If none of the specimens contain cancer (ie. DCIS) then reporting the margins becomes a moot point and the lumpectomy code is reduced to an 88305 also. This billing was reviewed and approved by an outside billing company and has been accepted by medicare and other insurances. That's all I have to go on. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 From: "Eck, Allison" I am looking for advice on a current billing problem. Breast specimens: We recieve the lumpectomy with orienting sutures - 88307 along with that are the accompanying margins (inferior, superior, medial, etc). Our breast surgeon has been placing orienting sutures on all of her margins. We had previously been billing under the assumption that the unsutured margins were 88305, but now with the sutures should we be billing an 88307 or still 88305 since they are margins? Thank you in advance for your help Allison ******** From smartin at lcpath.com Tue Jun 30 13:37:10 2015 From: smartin at lcpath.com (Suzanne Martin) Date: Tue, 30 Jun 2015 11:37:10 -0700 Subject: [Histonet] Trichrome troubleshooting In-Reply-To: <6B106EE8C8AAEF449DEA97921DEC11670E18DD4A66@EXMBOX-VP05.OSUMC.EDU> Message-ID: <51cf57d9ba6dce4bbdcf1bc7a3bebae3@mail2.lcpath.com> Hi all, We are having trouble troubleshooting our trichrome. It is too blue. We are using Leica's kit with the Weigerts iron with Gills. Most of the small bowel controls have seen improvement but patient tissue is not... strange. We have tried lessening the time in Gills, adding time for the last acid step, even lessening time and adding time in the Weigerts. Thoughts? Thank you. Suzanne HT From JGarrigan at buffalobiolabs.com Tue Jun 30 14:48:57 2015 From: JGarrigan at buffalobiolabs.com (Jennifer Garrigan) Date: Tue, 30 Jun 2015 19:48:57 +0000 Subject: [Histonet] Cost Efficient Embedding Center Message-ID: Hi All, 10/2013 we purchased the KD-BMII embedding center and cooling center. The cooling plate is still working fine, but our embedding center is already in need of so much repair that we are looking into replacing it instead. We are considering a different until because it is under 2 years old and needs most of the heating elements replaced. Do you have recommendations for a decent quality unit that is somewhere in the same ballpark? ($3200) We are a CRO and not doing much histology, but we are at a standstill until we get one. Any advice is appreciated! Jennifer Garrigan, LVT, AS, BA Senior Research Technician Buffalo BioLabs LLC 73 High Street Buffalo, NY 14203 (716)849-6810 x 357 jgarrigan at buffalobiolabs.com From liz at premierlab.com Tue Jun 30 15:29:56 2015 From: liz at premierlab.com (Elizabeth Chlipala) Date: Tue, 30 Jun 2015 14:29:56 -0600 Subject: [Histonet] Trichrome troubleshooting In-Reply-To: <51cf57d9ba6dce4bbdcf1bc7a3bebae3@mail2.lcpath.com> References: <6B106EE8C8AAEF449DEA97921DEC11670E18DD4A66@EXMBOX-VP05.OSUMC.EDU> <51cf57d9ba6dce4bbdcf1bc7a3bebae3@mail2.lcpath.com> Message-ID: <14E2C6176416974295479C64A11CB9AE02230F9D8F6C@SBS2K8.premierlab.local> Suzanne How many times have you used the kit and reagents, I did look up how the kit works but the trichrome stain can be tricky. First of all you need to make sure that the mordant (bouins solution) is at 60C prior to placing your slides in them. We normally heat up our bouins for at least an hour prior to placing the slides in the solution. We leave in bouins for an hour and a half rather than an hour. I see that this is a microwave protocol I cannot comment on that but I don't think that the hematoxylin is the issue, if you leave longer in 1% acetic acid that may pull some of the blue stain out or I would try dehydrating with lower alcohol percentage that can pull some of the blue stain out. I would also try leaving it a bit longer in the bouins after you microwave it - that might help. Trichrome staining works best with fresh reagents so if you have used these reagents too much that could cause problems. I'm also not a big fan of the one step trichromes, they are quicker but sometimes not as good as the two steps, just my opinion. FYI - to evaluate your staining look for a smaller vessel, the smooth muscle should be nice a red, if its greyish or blue you have not done the stain properly. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Suzanne Martin [mailto:smartin at lcpath.com] Sent: Tuesday, June 30, 2015 12:37 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Trichrome troubleshooting Hi all, We are having trouble troubleshooting our trichrome. It is too blue. We are using Leica's kit with the Weigerts iron with Gills. Most of the small bowel controls have seen improvement but patient tissue is not... strange. We have tried lessening the time in Gills, adding time for the last acid step, even lessening time and adding time in the Weigerts. Thoughts? Thank you. Suzanne HT _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis at bresnan.net Tue Jun 30 15:33:09 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Tue, 30 Jun 2015 14:33:09 -0600 Subject: [Histonet] Trichrome troubleshooting In-Reply-To: <51cf57d9ba6dce4bbdcf1bc7a3bebae3@mail2.lcpath.com> References: <6B106EE8C8AAEF449DEA97921DEC11670E18DD4A66@EXMBOX-VP05.OSUMC.EDU> <51cf57d9ba6dce4bbdcf1bc7a3bebae3@mail2.lcpath.com> Message-ID: <000701d0b373$facbde20$f0639a60$@bresnan.net> Suzanne, I don't understand what you wrote here " We are using Leica's kit with the Weigerts iron with Gills". In a long histo career, I never used Gills half oxidized hematoxylin for a trichrome stain nor heard of the combined staining you mentioned. Could you explain what you are doing here? Is this part of the Leica staining protocol? This is a new one for me to combine Gills hematoxylin with Weigerts iron hematoxylin and why it would even be necessary??? Both of Leica's trichromes kits use Weigerts Iron hematoxylin and do NOT have Gills in the method. Their kits look more like classic Mass Tri methods. Personally I would only use Weigerts Iron hematoxylin in a classic Trichrome method. It is important to know that iron hematoxylin is not stable over a long period of time since the ferric chloride continues to oxidize the hematoxylin so it becomes very weak or doesn't stain. We never used iron hematoxylin for more than one day although some people may have success over a week. We never took a chance in having weak staining with that continued oxidation by ferric chloride, and only used freshly mixed iron hematoxylin for a day. I would be happy to personally send you a modified Weigerts Iron hematoxylin that does NOT differentiate out so the iron hematoxylin staining is always excellent. It was found in a J of Histotechnology publication many years ago, and is made up in house - even using other trichrome reagents from the above vendors I mentioned. The modified Weigerts iron hematoxylin is more concentrated compared to the original iron hematoxylin recipe. Gayle M. Callis HTL/HT/MT(ASCP) -----Original Message----- From: Suzanne Martin [mailto:smartin at lcpath.com] Sent: Tuesday, June 30, 2015 12:37 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Trichrome troubleshooting Hi all, We are having trouble troubleshooting our trichrome. It is too blue. We are using Leica's kit with the Weigerts iron with Gills. Most of the small bowel controls have seen improvement but patient tissue is not... strange. We have tried lessening the time in Gills, adding time for the last acid step, even lessening time and adding time in the Weigerts. Thoughts? Thank you. Suzanne HT _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones at metropath.com Tue Jun 30 15:51:43 2015 From: mjones at metropath.com (Michael Ann Jones) Date: Tue, 30 Jun 2015 20:51:43 +0000 Subject: [Histonet] Trichrome troubleshooting Message-ID: Don?t know if this matters, but we have found that the trichrome stain fades on tissues that are not cut fresh. We use uterus as a control but they must be cut fresh, no batching this one. The trichrome is not as brilliant on pre-cut tissues. Just my 2 cents :) Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com On 6/30/15, 2:29 PM, "Elizabeth Chlipala" wrote: >Suzanne > >How many times have you used the kit and reagents, I did look up how the >kit works but the trichrome stain can be tricky. First of all you need >to make sure that the mordant (bouins solution) is at 60C prior to >placing your slides in them. We normally heat up our bouins for at least >an hour prior to placing the slides in the solution. We leave in bouins >for an hour and a half rather than an hour. I see that this is a >microwave protocol I cannot comment on that but I don't think that the >hematoxylin is the issue, if you leave longer in 1% acetic acid that may >pull some of the blue stain out or I would try dehydrating with lower >alcohol percentage that can pull some of the blue stain out. I would >also try leaving it a bit longer in the bouins after you microwave it - >that might help. > >Trichrome staining works best with fresh reagents so if you have used >these reagents too much that could cause problems. I'm also not a big >fan of the one step trichromes, they are quicker but sometimes not as >good as the two steps, just my opinion. > >FYI - to evaluate your staining look for a smaller vessel, the smooth >muscle should be nice a red, if its greyish or blue you have not done the >stain properly. Good Luck > >Liz > >Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC >Premier Laboratory, LLC >PO Box 18592 >Boulder, CO 80308 >(303) 682-3949 office >(303) 682-9060 fax >(303) 881-0763 cell >liz at premierlab.com >www.premierlab.com > >March 10, 2014 is Histotechnology Professionals Day > >Ship to Address: > >Premier Laboratory, LLC >1567 Skyway Drive, Unit E >Longmont, CO 80504 > > >-----Original Message----- >From: Suzanne Martin [mailto:smartin at lcpath.com] >Sent: Tuesday, June 30, 2015 12:37 PM >To: histonet at lists.utsouthwestern.edu >Subject: [Histonet] Trichrome troubleshooting > > >Hi all, > >We are having trouble troubleshooting our trichrome. It is too blue. We >are using Leica's kit with the Weigerts iron with Gills. Most of the >small bowel controls have seen improvement but patient tissue is not... >strange. > >We have tried lessening the time in Gills, adding time for the last acid >step, even lessening time and adding time in the Weigerts. > > >Thoughts? > >Thank you. > >Suzanne HT > > > > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gayle.callis at bresnan.net Tue Jun 30 16:05:39 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Tue, 30 Jun 2015 15:05:39 -0600 Subject: [Histonet] Trichrome troubleshooting In-Reply-To: <14E2C6176416974295479C64A11CB9AE02230F9D8F6C@SBS2K8.premierlab.local> References: <6B106EE8C8AAEF449DEA97921DEC11670E18DD4A66@EXMBOX-VP05.OSUMC.EDU> <51cf57d9ba6dce4bbdcf1bc7a3bebae3@mail2.lcpath.com> <14E2C6176416974295479C64A11CB9AE02230F9D8F6C@SBS2K8.premierlab.local> Message-ID: <000b01d0b378$853254a0$8f96fde0$@bresnan.net> Years ago, I was taught by Jerry Fredenburg, a stain guru, never to microwave the Bouins step but do this for 1 hour at 60C or overnight at RT. The post-fixation/mordant is very important in order to acidify the connective tissue fibers properly for a trichrome stain, and the short time in MW will not do the job. Liz is correct here in letting the sections stand longer in Bouins after microwaving or simply just do the 1 hour at 60C. I have removed Gills type hematoxylins from nuclei by over exposure to acetic acids so remember that ALL Masson's Trichrome reagents are acidified with acetic acid and will automatically do this, even on Weigert's Iron hematoxylin. We also examined our sections microscopically during staining to make sure the check the depth of red staining reagents on smooth muscle is correct - once again, Liz is correct about this fact. Gayle Callis -----Original Message----- From: Elizabeth Chlipala [mailto:liz at premierlab.com] Sent: Tuesday, June 30, 2015 2:30 PM To: Suzanne Martin; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Trichrome troubleshooting Suzanne How many times have you used the kit and reagents, I did look up how the kit works but the trichrome stain can be tricky. First of all you need to make sure that the mordant (bouins solution) is at 60C prior to placing your slides in them. We normally heat up our bouins for at least an hour prior to placing the slides in the solution. We leave in bouins for an hour and a half rather than an hour. I see that this is a microwave protocol I cannot comment on that but I don't think that the hematoxylin is the issue, if you leave longer in 1% acetic acid that may pull some of the blue stain out or I would try dehydrating with lower alcohol percentage that can pull some of the blue stain out. I would also try leaving it a bit longer in the bouins after you microwave it - that might help. Trichrome staining works best with fresh reagents so if you have used these reagents too much that could cause problems. I'm also not a big fan of the one step trichromes, they are quicker but sometimes not as good as the two steps, just my opinion. FYI - to evaluate your staining look for a smaller vessel, the smooth muscle should be nice a red, if its greyish or blue you have not done the stain properly. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Suzanne Martin [mailto:smartin at lcpath.com] Sent: Tuesday, June 30, 2015 12:37 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Trichrome troubleshooting Hi all, We are having trouble troubleshooting our trichrome. It is too blue. We are using Leica's kit with the Weigerts iron with Gills. Most of the small bowel controls have seen improvement but patient tissue is not... strange. We have tried lessening the time in Gills, adding time for the last acid step, even lessening time and adding time in the Weigerts. Thoughts? Thank you. Suzanne HT _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Tue Jun 30 18:46:36 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue, 30 Jun 2015 23:46:36 +0000 Subject: [Histonet] Suggestions for staining ground substances in the Heart In-Reply-To: <6014eb4d5aad426baf62e23118b93963@BN1PR26MB0001.067d.mgd.msft.net> References: <6014eb4d5aad426baf62e23118b93963@BN1PR26MB0001.067d.mgd.msft.net> Message-ID: <6D6BD1DE8A5571489398B392A38A7157F53FAB3D@xmdb04.nch.kids> If the ground substance and mucin are not staining, it would be a problem with the alcian blue solution. Microscopically check the slides after the alcian blue step - if no or weak staining then: 1. Is the alcian blue at pH 2.5 or is it pH1 - (goblet cells in stomach and ground substance rarely stain at pH 1)? 2. Is the alcian blue dye of good quality. There are several out there - use Certified dyes. Hopefully this helps Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Wolfe, Christina [mailto:christina.wolfe at bms.com] Sent: Wednesday, 1 July 2015 1:15 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Suggestions for staining ground substances in the Heart Hi all, We are interested in staining ground substances in the heart. Are there other stains that will work beside the Pentachrome? We have tried the Movat's pentachrome (commercial kit) and are able to demonstrate ground substance in the bone with the alcian blue part of this stain. In our hands the goblet cells of the gut and the ground substance in the heart are devoid of staining. We have tried sections cut at 4 and 6 microns. Any thoughts/suggestions? Kristie Christina Wolfe, BSHA, MLT (ASCP), HT, QIHC Drug Safety Evaluation/Bristol-Myers Squibb Pathology Dept. 812-307-2093 ________________________________ This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. *********************************************************************************