[Histonet] Breast Fixation

Garreyf garreyf at gmail.com
Wed Jul 29 16:39:28 CDT 2015


I agree.  That's the easiest solution.

My colleague at a sister institution does not allow breast excisions on Friday.

We need more people to do studies on fixation beyond 72 hours. My hunch is that another 24 hours won't affect ER , Pgr, and Her2 results.

Garrey

Sent from my iPhone

> On Jul 29, 2015, at 5:01 PM, Joanne Clark via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> 
> I would do a delayed start on your tissue processor Friday night to include the extra two hours you need of fixation time and just have the run come off two hours later on Saturday morning.  Just adjust the hours of your per diem Saturday tech to come in later.
> 
> Joanne Clark, HT
> Director of Histology
> Pathology Consultants of New Mexico
> 
> 
> Message: 4
> Date: Wed, 29 Jul 2015 08:01:31 -0700
> From: "Heckford, Karen - SMMC-SF" <Karen.Heckford at DignityHealth.org>
> To: "histonet at lists.utsouthwestern.edu"
>    <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Breast fixation
> Message-ID:
>    <D329219D3B967447A9E3ED3242117E3206FEDC7378 at PHX-MSG-007-N1.chw.edu>
> Content-Type: text/plain; charset="us-ascii"
> 
> Good Morning,
> I have a question about breast fixation.   I am in a little bit of a pickle with the 6-72 hour rule for the fixation on breast tissue.   Friday I am getting 2 breast cases in the afternoon and both will not have the required minimum 6 hour formalin fixation for my per diem to cut early Saturday morning.   He will not be able to make it in again until Monday night.  The tissue will be about 3-4 hours (this includes time on the processor)  over the 72 hour maximum.    Does anyone have any suggestions on what can be done?  We are a one person show here.
> 
> Thanks,
> 
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> karen.heckford at dignityhealth.org
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> ------------------------------
> 
> Message: 5
> Date: Wed, 29 Jul 2015 12:10:19 -0300
> From: Emily Dewar <emilydewar32144 at gmail.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] NetWell inserts and IHC with TUNEL stain
> Message-ID:
>    <CAJ7MPPp=h9pgEkH-SxJG=KkDiDxi4=6_TTrAP7RCb5NSJnm47A at mail.gmail.com>
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> 
> Hello,
> 
> I will be performing a number of immunos within the next couple of months,
> and have been wondering what the best way to do so might be with no tissue
> damage during the process. Does anyone know whether using NetWell inserts
> for immunos to transfer tissue affects morphology? The tissue will be
> placed into the insert, and submersed in solution within a well plate to be
> incubated on a shaker. The problem is that contact with the NetWell insert
> could damage the tissue, not only with the rocking, but with transfer from
> one solution to another.
> 
> I am under the impression that with TUNEL staining, is often difficult to
> differentiate whether the damage in cells caused by handling/extraction, or
> from the treatment itself. While not impossible, I will not have the time
> to develop or perform such a procedure.
> 
> If anyone has any knowledge or insight, it would be greatly appreciated.
> 
> Thank you for your time,
> 
> Emily Dewar
> Laboratory Technician
> 
> 
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