[Histonet] Re. Decalcification with formic acid sodium

[Hobbs, Carl]

Mouse knee joints:
done lots of decalcified FFPWS for assessment of articular cartilage degeneration models.
See Histonet images for a Tol blue image.
Decal in 10 % EDTA for 3 days on a rocker at RT.
Sure....5days if you are worried.
No difference in Immuno-reactivity, imho.
If you want to use buffered Formic acid, use Formic acid; sodium formate.
Use of citric acid with Formic acid does not make a buffer.
It's just mixing two relatively mild acids.....
However, I am sure that Prof Kiernan can further enlighten us.

Respectfully,
 
Carl Hobbs FIBMS 
Histology and Imaging Manager 
Wolfson CARD 
Guys Campus, London Bridge  
Kings College London 
London 
SE1 1UL 
  
020 7848 6813