From b-frederick at northwestern.edu Wed Jul 1 07:28:06 2015 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Wed, 1 Jul 2015 12:28:06 +0000 Subject: [Histonet] Trichrome troubleshooting In-Reply-To: <14E2C6176416974295479C64A11CB9AE02230F9D8F6C@SBS2K8.premierlab.local> References: <6B106EE8C8AAEF449DEA97921DEC11670E18DD4A66@EXMBOX-VP05.OSUMC.EDU> <51cf57d9ba6dce4bbdcf1bc7a3bebae3@mail2.lcpath.com> <14E2C6176416974295479C64A11CB9AE02230F9D8F6C@SBS2K8.premierlab.local> Message-ID: Actually, I have microwaved the Bouins (and still do) for small numbers of slides and the results are the same. I ran a liver bx both ways as well as larger tissue to compare. I use the 10 slide plastic coplin jar and generally have 5 or less slides when I do this. One microwaves the Bouins for 30 seconds on power level 7 in a lab grade microwave. A higher level will cause the Bouin's to spill. Slides are then added and left for 5 minutes. Excess rinsed out and then proceed as per your SOP. As for the blue- I rinse out excess and do 1 dip in 1% GAA. Rinse and dehydrate (10 dips each solution) Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu -----Original Message----- From: Elizabeth Chlipala [mailto:liz at premierlab.com] Sent: Tuesday, June 30, 2015 3:30 PM To: Suzanne Martin; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Trichrome troubleshooting Suzanne How many times have you used the kit and reagents, I did look up how the kit works but the trichrome stain can be tricky. First of all you need to make sure that the mordant (bouins solution) is at 60C prior to placing your slides in them. We normally heat up our bouins for at least an hour prior to placing the slides in the solution. We leave in bouins for an hour and a half rather than an hour. I see that this is a microwave protocol I cannot comment on that but I don't think that the hematoxylin is the issue, if you leave longer in 1% acetic acid that may pull some of the blue stain out or I would try dehydrating with lower alcohol percentage that can pull some of the blue stain out. I would also try leaving it a bit longer in the bouins after you microwave it - that might help. Trichrome staining works best with fresh reagents so if you have used these reagents too much that could cause problems. I'm also not a big fan of the one step trichromes, they are quicker but sometimes not as good as the two steps, just my opinion. FYI - to evaluate your staining look for a smaller vessel, the smooth muscle should be nice a red, if its greyish or blue you have not done the stain properly. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Suzanne Martin [mailto:smartin at lcpath.com] Sent: Tuesday, June 30, 2015 12:37 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Trichrome troubleshooting Hi all, We are having trouble troubleshooting our trichrome. It is too blue. We are using Leica's kit with the Weigerts iron with Gills. Most of the small bowel controls have seen improvement but patient tissue is not... strange. We have tried lessening the time in Gills, adding time for the last acid step, even lessening time and adding time in the Weigerts. Thoughts? Thank you. Suzanne HT _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Wed Jul 1 12:20:29 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 1 Jul 2015 17:20:29 +0000 Subject: [Histonet] Trichrome troubleshooting In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F801E0AB@HRHEX02-HOS.holyredeemer.local> Please clarify if you are referring to the Aniline blue, or the Hematoxylin blue. I, also, have never heard of using Gills in association with any Trichrome stain, but as a long time user and educator of using a one-step (Gomori's) trichrome stain (which is the Leica blue collagen stain), if you rinse with any water between the stain step and the acetic acid step, you will end up with a stain that is overpoweringly blue. Here are the steps copied from the Leica blue collagen kit instructions. Notice NO WATER RINSE between the stain and Acetic Acid Solution. It is a common mistake. 10. Gently mix the Gomori's Trichrome Blue Solution by swirling and allow to stand for 6 minutes. 11. Place sections in 1% Acetic Acid solution for 1 minute. I hope this helps. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 2. Trichrome troubleshooting (Suzanne Martin) From: "Suzanne Martin" Hi all, We are having trouble troubleshooting our trichrome. It is too blue. We are using Leica's kit with the Weigerts iron with Gills. Most of the small bowel controls have seen improvement but patient tissue is not... strange. We have tried lessening the time in Gills, adding time for the last acid step, even lessening time and adding time in the Weigerts. Thoughts? Thank you. Suzanne HT From esarricks at gmail.com Wed Jul 1 13:39:59 2015 From: esarricks at gmail.com (Erin Sarricks) Date: Wed, 1 Jul 2015 14:39:59 -0400 Subject: [Histonet] Searching for Horizontal Slide Racks Message-ID: Hi Histonet- Does anyone have any slide racks that hold the slides horizontally that they no longer need? Like this but for maybe 50-60 slides? http://grale.com.au/products/view/297 I am willing to purchase and pay for shipping if anyone has any they are getting rid of. Thank you! Erin From Lea.Alminde at tuhs.temple.edu Wed Jul 1 16:51:28 2015 From: Lea.Alminde at tuhs.temple.edu (Alminde, Lea S) Date: Wed, 1 Jul 2015 21:51:28 +0000 Subject: [Histonet] Unsubscribe Message-ID: <5BBDB50A8DDE564B98D4FE3C84AAD3AA2034580C@Tsexmb5.tuhs.prv> Lea S. Alminde Anatomic Pathology Supervisor Jeanes Hospital 215-728-2034 email lea.alminde at tuhs.temple.edu ________________________________ This electronic message is intended to be for the use of the named recipient, and may contain information that is confidential or privileged. This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Pennsylvania Laws. You can direct questions concerning PHI or HIPAA to the Corporate Compliance and Privacy Officer at (215) 707-5605. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete and destroy all copies of this message. From Joyce.Weems at emoryhealthcare.org Wed Jul 1 17:05:35 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Wed, 1 Jul 2015 22:05:35 +0000 Subject: [Histonet] Unsubscribe In-Reply-To: <5BBDB50A8DDE564B98D4FE3C84AAD3AA2034580C@Tsexmb5.tuhs.prv> References: <5BBDB50A8DDE564B98D4FE3C84AAD3AA2034580C@Tsexmb5.tuhs.prv> Message-ID: Hello Lea, You must go to the website listed at the bottom of the email and unsubscribe. Here it is... http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best wishes, Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Alminde, Lea S [mailto:Lea.Alminde at tuhs.temple.edu] Sent: Wednesday, July 01, 2015 5:51 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Unsubscribe Lea S. Alminde Anatomic Pathology Supervisor Jeanes Hospital 215-728-2034 email lea.alminde at tuhs.temple.edu ________________________________ This electronic message is intended to be for the use of the named recipient, and may contain information that is confidential or privileged. This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Pennsylvania Laws. You can direct questions concerning PHI or HIPAA to the Corporate Compliance and Privacy Officer at (215) 707-5605. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete and destroy all copies of this message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From relia1 at earthlink.net Wed Jul 1 23:26:55 2015 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 2 Jul 2015 00:26:55 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin 7-02-2015 Have a Safe and Happy 4th of July Holiday!! Message-ID: <000001d0b47f$54616660$fd243320$@earthlink.net> Hi Histonetters!!! I hope you Have a safe and Happy July 4th Weekend. I Have A Fun Idea to Share for Pool Parties And Cookouts. Sprinkle Red Pop Rocks And Blue Sprinkles On Iced Sugar Cookies To Make Edible Firecrackers!! I have some exciting opportunities that I want to pass along as well. Maybe they will look good to you or someone you know. RELIA'S Hot Histology Opportunities!! Supervisor of IHC - Long Island, NY A RELIA EXCLUSIVE!!!!! Lead Histotech - Great Falls, MT A RELIA Exclusive! Histotech - Bellevue, WA Histotechnician - Norfolk, VA $15K Sign-on bonus!!!! Histotechnician - Atlanta, GA Histology Tech - Asheville, NC IHC Tech - Waynesboro, VA Histotechnician - Nashville, TN Histology Equipment Repair - Tampa Bay, FL All of these jobs are full time permanent positions with some of the finest facilities in the country. My clients offer excellent compensation and benefits and most offer relocation assistance or sign- on bonuses. For more information for you or a friend please call me at the office at 866-607-3542 or on my cell at 407-353-5070 or shoot me at email at Relia1 at earthlink.net Have a Safe and Happy 4th of July!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From jkiernan at uwo.ca Thu Jul 2 00:49:40 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Thu, 02 Jul 2015 00:49:40 -0500 Subject: [Histonet] Suggestions for staining ground substances in the Heart In-Reply-To: <73b0ca0f1699d.5594d0d9@uwo.ca> References: <6014eb4d5aad426baf62e23118b93963@BN1PR26MB0001.067d.mgd.msft.net> <7350ab1510d3e.5594ba5c@uwo.ca> <7200ccdf1354f.5594bc3c@uwo.ca> <72009f3a11187.5594bc7a@uwo.ca> <7200c33b16131.5594bcb8@uwo.ca> <72f0dd0114575.5594c040@uwo.ca> <72f08f8c150ad.5594c07e@uwo.ca> <7280d07f111d3.5594c0bc@uwo.ca> <7300df2e14f68.5594c228@uwo.ca> <72c0ab6f178be.5594c2a2@uwo.ca> <73b0f013151ea.5594c2e2@uwo.ca> <72c0ac6617154.5594c320@uwo.ca> <73309c111083a.5594c505@uwo.ca> <73b086bb115d1.5594c543@uwo.ca> <72c09c9a17c47.5594c581@uwo.ca> <7300c8ac14132.5594c5c0@uwo.ca> <7300a8a2106af.5594c5fe@uwo.ca> <7300da7d123f9.5594c63c@uwo.ca> <7300ef711190e.5594c67a@uwo.ca> <7330922e1332b.5594c6b8@uwo.ca> <7330b79311172.5594c6f6@uwo.ca> <73309e2a177c7.5594c735@uwo.ca> <7330a5ee13d3a.5594c774@uwo.ca> <7330f9d513d71.5594c7b2@uwo.ca> <7330fbed13625.5594c7f0@uwo.ca> <7300fd8011a9c.5594c82f@uwo.ca> <7300a8aa11d29.5594c8a9@uwo.ca> <7300feb812180.5594c8e7@uwo.ca> <7300f8df14308.5594c926@uwo.ca> <7300cc8410e1e.5594c964@uwo.ca> <73008a3716144.5594c9a2@uwo.ca> <73e0f190139de.5594c9e0@uwo.ca> <7350b14a140e8.5594ca98@uwo.ca> <73509ff512189.5594cad6@uwo.ca> <72e0a7931504c.5594cb8d@uwo.ca> <72e0ef2a15d35.5594cc43@uwo.ca> <72e093b412b00.5594cc81@uwo.ca> <72e0a828127f3.5594ccbf@uwo.ca> <72e08c3012ff7.5594ccfd@uwo.ca> <72e08dc410a67.5594cd3b@uwo.ca> <73e0f22210744.5594ce2e@uwo.ca> <72d0b6ec126e9.5594ce6c@uwo.ca> <728097a910506.5594ceaa@uwo.ca> <7280e47e1433a.5594cee8@uwo.ca> <7300ff3d142b4.5594cf26@uwo.ca> <73e092841096b.5594cf64@uwo.ca> <7350875310994.5594cfa2@uwo.ca> <73b0ee9916fc1.5594cfe0@uwo.ca> <73b098ba12f59.5594d01e@uwo.ca> <73b0a57012481.5594d05c@uwo.ca> <73b0f14a17665.5594d09b@uwo.ca> <73b0ca0f1699d.5594d0d9@uwo.ca> Message-ID: <73e0d55a14688.55948aa4@uwo.ca> Movat's pentachrome is unnecessarily complicated when you want to look only at one component of a tissue. Most "ground substance" (extracellular material between cells, collagen fibres etc) is weakly stainable with PAS, alcian blue pH2.5 or both. It's usual to stain with alcian blue first (Mowry's method). If you do the PAS first, the PAS-positive materials will also stain with alcian blue and be darker. For the reasons, see: Johannes, M.-L. and Klessen, C. (1984). Alcian blue/PAS or PAS/alcian blue? Remarks on a classical technique used in carbohydrate histochemistry. Histochemistry 80:129-132. Yamabayashi, S. (1987). Periodic acid-Schiff-alcian blue: a method for the differential staining of glycoproteins. Histochemical Journal 19:565-571. Reid, P.E. and Owen, D.A. (1988). Some comments on the mechanism of the periodic acid-Schiff-Alcian blue method. Histochemical Journal 20:651-654. If you can predict the PAS-AB effect without further reading, you already have a good understanding of how the traditional methods of carbohydrate histochemistry work! John Kiernan London, Canada = = = On 30/06/15, "Wolfe, Christina" wrote: > Hi all, > We are interested in staining ground substances in the heart. Are there other stains that will work beside the Pentachrome? We have tried the Movat's pentachrome (commercial kit) and are able to demonstrate ground substance in the bone with the alcian blue part of this stain. In our hands the goblet cells of the gut and the ground substance in the heart are devoid of staining. We have tried sections cut at 4 and 6 microns. Any thoughts/suggestions? > > Kristie > > Christina Wolfe, BSHA, MLT (ASCP), HT, QIHC > Drug Safety Evaluation/Bristol-Myers Squibb > Pathology Dept. > 812-307-2093 > > > ________________________________ > This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jkiernan at uwo.ca Thu Jul 2 01:09:56 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Thu, 02 Jul 2015 01:09:56 -0500 Subject: [Histonet] Alcian blue (was "Suggestions for staining ground substances ...") In-Reply-To: <72d08b6b102f7.5594d570@uwo.ca> References: <6014eb4d5aad426baf62e23118b93963@BN1PR26MB0001.067d.mgd.msft.net> <73e0b19916ed4.5594d16e@uwo.ca> <73e0efb4105d2.5594d1ab@uwo.ca> <73e0cdc21507f.5594d1e9@uwo.ca> <73e091b213845.5594d227@uwo.ca> <73e0dd1c117e3.5594d265@uwo.ca> <73e0e78e17006.5594d2a3@uwo.ca> <73e0808f173dc.5594d2e2@uwo.ca> <73e0c17710f68.5594d320@uwo.ca> <73e081291042f.5594d35e@uwo.ca> <73e0b5c91162c.5594d3fe@uwo.ca> <73e0c314177a9.5594d478@uwo.ca> <73e0d07b1629c.5594d4b6@uwo.ca> <7350d34b11a89.5594d4f4@uwo.ca> <72c0eed6108e1.5594d532@uwo.ca> <72d08b6b102f7.5594d570@uwo.ca> Message-ID: <7200bba415465.55948f64@uwo.ca> Dear Histonetters, I recently replied to a question about staining extracellular "ground substance" with alcian blue. Here is some more information. With the alcian blue-PAS sequence it is necessary to have a good alcian blue dye - the same as or functionally equivalent to the original alcian blue 8G. For the PAS-alcian blue sequence, other alcian blues may also be OK. Alcian blue from a batch certified (or rechecked) by the Biological Stain Commission (BSC) 5 or fewer years ago should be good for either procedure. Of the dyes commonly used to make up staining solutions for microscopy, alcian blue is the only only one that sometimes deteriorates, unpredictably and often quite quickly, as a dry powder in the bottle. Some alcian blue powders from the 1950s are still OK, but some very much younger ones are no good 5+ years after passing the BSC's analytical and staining tests. John Kiernan London, Canada http://biostain.com = = = From bburnett at CapeCodHealth.org Thu Jul 2 07:31:34 2015 From: bburnett at CapeCodHealth.org (Burnett, Brandy) Date: Thu, 2 Jul 2015 12:31:34 +0000 Subject: [Histonet] Ventana LCS (Liquid Cover Slip) Message-ID: Anyone out there having any issues with the Ventana LCS? When you are cover slipping are you seeing any water/residual LCS on the slides? I was wondering if we should dry our slides before cover slipping instead of running them down? Any information would be greatly appreciated! Brandy Burnett Histotechnologist, QIHC(ASCP) CCH Pathology/Histology bburnett at CapeCodHealth.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk at CapeCodHealth.org From TNMayer at mdanderson.org Thu Jul 2 09:57:04 2015 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Thu, 2 Jul 2015 14:57:04 +0000 Subject: [Histonet] Suggestions for staining ground substances in the Heart Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC88224BEFAA@D1PWPEXMBX05.mdanderson.edu> Kristie, For heart you could try the elastic Trichrome, it will stain the ground substances and is faster than the Movat. If your ground substance is devoid of staining it could be the phosphotungstic acid. Is it 5%, and fresh? Are you using two changes at 5 min each? Continue with the 4micron sections, and use fresh solutions. If you can make the phosphotungstic yourself, do that. Commercial solutions sometimes do not store well. Hope this helps. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 -----Original Message----- From: Wolfe, Christina [mailto:christina.wolfe at bms.com] Sent: Wednesday, 1 July 2015 1:15 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Suggestions for staining ground substances in the Heart Hi all, We are interested in staining ground substances in the heart. Are there other stains that will work beside the Pentachrome? We have tried the Movat's pentachrome (commercial kit) and are able to demonstrate ground substance in the bone with the alcian blue part of this stain. In our hands the goblet cells of the gut and the ground substance in the heart are devoid of staining. We have tried sections cut at 4 and 6 microns. Any thoughts/suggestions? Kristie Christina Wolfe, BSHA, MLT (ASCP), HT, QIHC Drug Safety Evaluation/Bristol-Myers Squibb Pathology Dept. 812-307-2093 From julie at seascapesurgerycenter.com Thu Jul 2 10:17:10 2015 From: julie at seascapesurgerycenter.com (Julie) Date: Thu, 2 Jul 2015 11:17:10 -0400 Subject: [Histonet] Histotech position in Tampa, FL!! Message-ID: <001601d0b4da$2af11fe0$80d35fa0$@seascapesurgerycenter.com> Our state-of-the-art dermatologic surgery practice in New Tampa is seeking a motivated candidate with professionalism, attention to detail, and a strong work ethic to join our team! Beautiful work environment and great benefits. Responsibilities include Mohs specimen (frozen section) preparation as well as permanent section preparation. Experience with Mohs and/or immunohistochemistry isn't required, but is a plus! Monday through Friday position with hours worked falling between 8am and 6pm Please send resumes to Julie at seascapesurgerycenter.com Julie The Bowman Institute Seascape Surgery Center Phone: (813) 977-2040 Fax: (813) 977-3886 From LBUSTAMANTE at cvm.tamu.edu Thu Jul 2 11:00:42 2015 From: LBUSTAMANTE at cvm.tamu.edu (Bustamante, Lin) Date: Thu, 2 Jul 2015 16:00:42 +0000 Subject: [Histonet] Distain Masson Trichrome Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC901560AE637@CVMMB02.cvm.tamu.edu> Could you please let me know what % of Ammonia water is used for distain Masson Trichrome? Or what % is most common used? Thank you. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 From rjbuesa at yahoo.com Thu Jul 2 11:28:59 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 2 Jul 2015 16:28:59 +0000 (UTC) Subject: [Histonet] Distain Masson Trichrome In-Reply-To: <94B6DC15AAF2F046BF847D4C1CA9AAC901560AE637@CVMMB02.cvm.tamu.edu> References: <94B6DC15AAF2F046BF847D4C1CA9AAC901560AE637@CVMMB02.cvm.tamu.edu> Message-ID: <1668451663.1084812.1435854540018.JavaMail.yahoo@mail.yahoo.com> Usually the "strong" 28% is used but I always prefer using a diluted solution so I have more control on the distaining.Ren? On Thursday, July 2, 2015 12:10 PM, "Bustamante, Lin" wrote: Could you please let me know what % of Ammonia water is used for distain Masson Trichrome? Or what % is most common used? Thank you. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree at uwhealth.org Thu Jul 2 12:20:07 2015 From: LSebree at uwhealth.org (Sebree Linda A) Date: Thu, 2 Jul 2015 17:20:07 +0000 Subject: [Histonet] Ventana LCS (Liquid Cover Slip) In-Reply-To: References: <77DD817201982748BC67D7960F2F76AF14B877@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: <77DD817201982748BC67D7960F2F76AF14B8E6@UWHC-MBX12.uwhis.hosp.wisc.edu> You certainly can air/oven dry your slides instead of running them down and coverslipping; we do that with the slides we take off in the morning when we get in. As to your problem with water/oil on your slides, something is not right in your dawn water rinsing, dehydration and/or clearing steps...but I'm sure I'm not telling you anything you don't know already. I would change out all the graded alcohols and xylenes so you know the right reagents are in your containers. Next, I would make sure that whomever is rinsing the slides, that they are using warm or even hot Dawn water and that they are rinsing long enough for your DAB slides. If you're also running APR you may want to do the air/oven drying to xylene method to maintain the chromogen binding. Other than these steps, I don't know what else to suggest. A long, long time ago when I worked in an old lab with water chilled pipes for air conditioning, I ran into a lot of humidity in the summer and actually had to add molecular sieve to my 100% alcohol in my dehydration set-up or my xylenes would get contaminated with water but we're talking the mid 70's so I'm hoping you're not running into something similar. Hope this helps, Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Burnett, Brandy [mailto:bburnett at CapeCodHealth.org] Sent: Thursday, July 02, 2015 12:07 PM To: Sebree Linda A Subject: RE: Ventana LCS (Liquid Cover Slip) For IHC..We are rinsing them with dawn before running them down. Brandy Burnett Histotechnologist, QIHC(ASCP) CCH Pathology/Histology 508-862-5267 bburnett at capecodhealth.org Expert physicians. Quality hospitals. Superior care. -----Original Message----- From: Sebree Linda A [mailto:LSebree at uwhealth.org] Sent: Thursday, July 02, 2015 10:17 AM To: Burnett, Brandy Subject: RE: Ventana LCS (Liquid Cover Slip) Are you talking about IHC/ISH or Specials Brandy? Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Burnett, Brandy [mailto:bburnett at CapeCodHealth.org] Sent: Thursday, July 02, 2015 7:32 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Ventana LCS (Liquid Cover Slip) Anyone out there having any issues with the Ventana LCS? When you are cover slipping are you seeing any water/residual LCS on the slides? I was wondering if we should dry our slides before cover slipping instead of running them down? Any information would be greatly appreciated! Brandy Burnett Histotechnologist, QIHC(ASCP) CCH Pathology/Histology bburnett at CapeCodHealth.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk at CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk at CapeCodHealth.org From anna.coffey at nih.gov Thu Jul 2 13:55:42 2015 From: anna.coffey at nih.gov (Coffey, Anna (NIH/NCI) [C]) Date: Thu, 2 Jul 2015 18:55:42 +0000 Subject: [Histonet] Cytologic and Frozen Specimens - IHC Techniques Message-ID: <5C3E10119A1B824FBE92B08279F74A9102597C76@msgb10.nih.gov> Is anyone able to recommend a good reference on preparation techniques for immunostaining of cytologic and frozen specimens? I'm studying for the qIHC exam and I'm striking out with my usual sources (Carson, DAKO guide, etc.) on some of the more specific questions in these areas. Thanks! Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey at nih.gov 301-846-1730 From ttroyer at petersonlab.com Thu Jul 2 14:39:41 2015 From: ttroyer at petersonlab.com (Travis Troyer) Date: Thu, 2 Jul 2015 14:39:41 -0500 Subject: [Histonet] Humidity Message-ID: <000101d0b4fe$d74f3690$85eda3b0$@petersonlab.com> I have had an issue on a couple of occasions now. Our lab gets really humid and when I have a block that needs re-embedded, the tissue becomes saturated with water and difficult to cut. The tissue seemed properly processed before, but after embedding, it almost peels out of the block. Has anyone else had this problem and is so, do you have a solution. Thanks, Travis Troyer Peterson Laboratory Services Manhattan, KS From i.olowookere at yahoo.com Thu Jul 2 21:56:50 2015 From: i.olowookere at yahoo.com (Isaac Olowookere) Date: Thu, 2 Jul 2015 19:56:50 -0700 Subject: [Histonet] Looking for Opportunity in Canada Message-ID: <1435892210.75827.BPMail_high_carrier@web120106.mail.ne1.yahoo.com> Hi, I hope you guys are doing well. I am looking for a job opportunity in Canada and I will really appreciate it if you guys could help me. I am an Histotech. With both HT(ASCP)and HTL(ASCP)Certifications. I also have both QIHC(ASCP) and QLS(ASCP)Qualifications. Thanks, Isaac From KSimeone at leavittmgt.com Mon Jul 6 08:21:21 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Mon, 6 Jul 2015 13:21:21 +0000 Subject: [Histonet] FT EVENING (3rd shift) 6p-2a POSITION DELRAY BCH FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C3E6380DF@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Dermatology Lab. This is a permanent full time EVENING SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality assessment and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengimann at leavittmgt.com if interested. *full time position Mon-Fri or Sun-Thurs 6PM-2:30AM *MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo half of your shift) *MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE! *VERY proficient in embedding and microtomy *must be self motivated, reliable and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) *some IHC experience preferred Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From akbitting at geisinger.edu Mon Jul 6 09:11:27 2015 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Mon, 6 Jul 2015 14:11:27 +0000 Subject: [Histonet] Old microtome knives Message-ID: For anyone out there still using the old microtome knives that you have to sharpen yourself- I have a box full, maybe 20 knives that are "free to a good home". Contact me off line if you are interested. Warmest regards, Angie Angela K. Bitting, HT(ASCP),QIHC Technical Specialist for Special Stains/ IHC/ISH Geisinger Health System 100 N. Academy Avenue Danville, PA 17822-2300 t. 570-214-9634 f. 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From carol.wilson at ricerca.com Mon Jul 6 09:32:01 2015 From: carol.wilson at ricerca.com (Wilson, Carol) Date: Mon, 6 Jul 2015 10:32:01 -0400 Subject: [Histonet] Alizarin Red for rodent Feti Message-ID: <19848F1C0886A5409C729CC2136EDAFF601B29FC45@IAD2MBX09.mex02.mlsrvr.com> Good Day All, Can someone pass along methodology for processing rodent Feti for Alizarin Red stain and exam? I would appreciate any input/ direction given as this is a foreign topic to me. Thanks, Carol Carol Wilson, HT(ASCP) Associate Scientist III Team Leader/Histopathology Ricerca Biosciences, LLC From carol.wilson at ricerca.com Mon Jul 6 09:50:08 2015 From: carol.wilson at ricerca.com (Wilson, Carol) Date: Mon, 6 Jul 2015 10:50:08 -0400 Subject: [Histonet] Alizarin Red for rodent feti Message-ID: <19848F1C0886A5409C729CC2136EDAFF601B29FC49@IAD2MBX09.mex02.mlsrvr.com> Hi All, Going in a different direction in this area, are there any reference labs out there that can process/examine these specimens? Thanks, Carol Carol Wilson, HT(ASCP) Associate Scientist III Team Leader/Histopathology Ricerca Biosciences, LLC From jkiernan at uwo.ca Mon Jul 6 11:43:30 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Mon, 06 Jul 2015 11:43:30 -0500 Subject: [Histonet] Alizarin Red for rodent Feti In-Reply-To: <7350d6b2ef1d.559ab028@uwo.ca> References: <19848F1C0886A5409C729CC2136EDAFF601B29FC45@IAD2MBX09.mex02.mlsrvr.com> <7380b857e8c0.559aaef2@uwo.ca> <7310959fc978.559aaf2f@uwo.ca> <7350ffacab4b.559aaf6e@uwo.ca> <73808332dc5d.559aafea@uwo.ca> <7350d6b2ef1d.559ab028@uwo.ca> Message-ID: <7330c8c6ecb8.559a69e2@uwo.ca> Red bone and blue cartilage: Webb, G.N. and Byrd, R.A. (1994). Simultaneous differential staining of cartilage and bone in rodent fetuses: an alcian blue and alizarin red S procedure without glacial acetic acid. Biotechnic & Histochemistry 69:181-185. Redfern, B.G., Wise, L.D. and Spence, S. (2007). An alternative alcian blue dye variant for the evaluation of fetal cartilage. Birth Defects Research (Part B) 80:171-176. Yamazaki, Y., Yuguchi, M., Kubota, S. and Isokawa, K. (2011). Whole-mount bone and cartilage staining of chick embryos with minimal decalcification. Biotechnic & Histochemistry 86:351-358. Trueman, D. and Stewart, J. (2014). An automated technique for double staining mouse fetal and neonatal skeletal specimens to differentiate bone and cartilage. Biotechnic & Histochemistry 89:315-319. John Kiernan London, Canada P.S. Fetus is a 4th declension Latin noun, so its plural in English is fetuses, not feti! = = = On 06/07/15, "Wilson, Carol" wrote: > Good Day All, > Can someone pass along methodology for processing rodent Feti for Alizarin Red stain and exam? I would appreciate any input/ direction given as this is a foreign topic to me. > Thanks, > Carol > > Carol Wilson, HT(ASCP) > Associate Scientist III > Team Leader/Histopathology > Ricerca Biosciences, LLC > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From SPINHEIRO at lumc.edu Mon Jul 6 13:28:21 2015 From: SPINHEIRO at lumc.edu (STEVEN PINHEIRO) Date: Mon, 6 Jul 2015 18:28:21 +0000 Subject: [Histonet] Specimens lost during processing. Message-ID: All, Looking for help in analyzing the entire scope of the process. There is not much published data (that I can find) and I am hoping this group can lend some expertise. Our rate is higher than we would like it to be. There is no consistent size at risk although GI and Derm biopsies are the biggest involved group. We have broken it down into steps. 1. Can be lost at grossing- either never loaded into the cassette at all, or cassette was discarded. Thus we hold on to our waste and can search for misdirected cassettes if need be. 2. Lost in the processor itself. Most are wrapped. If large enough not to be wrapped, we would not expect the processor to eat them, so assume cassette lid not properly closed. Frankly the highest number of losses we're seeing is no tissue found in cassette by embedders. 3. I am being told that we can't use micromesh cassettes in our microwave processors (Milestone Pathos) and want to know if anyone is. 4. Tissue not seen at embedding. Again no way to tell when the tissue disappeared. We know that tiny tissue can spring out during the opening at embedding but I don't know how else to examine or limit this step. 5. Tissue can be exhausted during microtomy. Rare but noteworthy. I am hoping people can tell me about their procedures for dealing with "specimens that don't survive processing", what safeguards they have in place, and to some extent what your own lab percentage or experience is. Apologies in advance for the length of the message, but could really use your help. Steven Pinheiro, MBA, MLS(ASCP)DLMCM Manager Anatomic Pathology and Cytology Loyola University Medical Center 2160 S First Ave, Bldg 110 Rm 2214 Maywood, IL 60153 708-327-2642 (O) 708-327-2620 (F) spinheiro at lumc.edu "You must do the thing you think you cannot do" E. Roosevelt Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Timothy.Morken at ucsf.edu Mon Jul 6 14:39:35 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 6 Jul 2015 19:39:35 +0000 Subject: [Histonet] Preventing Bubbles in Alkaline Phosphatase Message-ID: <761E2B5697F795489C8710BCC72141FF602F63AC@ex07.net.ucsf.edu> All knowing Histonet.... We started doing alkaline phosphatase on muscle frozen a little while ago and have had an issue with apparent air bubbles forming over the tissue. Not trapped air from coverslipping, but forming from apparent reaction in the tissue. Does anyone have experience with this and a solution? Images: http://histosearch.com/imageupload/alkaline-phosphatase-bubbles/ We tried longer formalin fixation after the stain (10 min) and an acetic acid rinse after the tap water, after the formalin. Still the same problem The only mention I have found about this is a Histochemistry text by Lojda from 1979 that says to fix in formalin for several hours after staining to prevent bubble formation. Does anyone have anything shorter? I don't really remember seeing this when I did these stains 20 years ago.... Our procedure (from a method given to our neuropath folks by a group in Australia): Frozen sections of muscle Borate Buffer, pH 8.8: 0.992 g Boric Acid 2.28 g Sodium Tetraborate 200 ml distilled water Mix well. Adjust to pH 8.8. Store at 4?C. 0.1M Magnesium Sulphate 0.6 g Magnesium sulphate, anhydrous (M7506-500G) 50 ml distilled water Store at 4?C. ALP Incubation Solution: 15 ml Borate Buffer 2 ml 0.1M Magnesium Sulphate 16.5 mg 1-naphthyl phosphate 16.5 mg Fast Blue RR Mix in well order. Filter. Glycerin Jelly Mounting Media 1. Place glycerin jelly in 60?C oven to liquify 2. Air dry slides 15 minutes. 3. Incubate in filtered ALP Incubation Solution at 37?C...45 minutes. 4. Rinse in tap water. 5. Change to 10% formalin...1 min 6. Rinse in tap water. 7. Air dry. 8. Coverslip with Glycerin Jelly. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.morken at ucsfmedctr.org From Clough at medicine.tamhsc.edu Mon Jul 6 22:05:14 2015 From: Clough at medicine.tamhsc.edu (Clough, Bret) Date: Tue, 7 Jul 2015 03:05:14 +0000 Subject: [Histonet] Histology on Rat spine. Message-ID: <28529330310DFE4A9F6FFF708AEB090C552CC8CA@CSR-Mail1.ad.tamhsc.edu> Hello everyone, I have some rat spines that I need to decal, process, and embed for sectioning. Is there someone in the histonet community that would be willing to either share with me their protocol or at least give me guidance with these spines. These are larger than any tissue that I have ever had to process. The spines have been fixed in Carson's fixative. Any help would greatly be appreciated. Sincerely, Bret Clough Texas A&M Health Science Center Temple, Texas From sprice2003 at gmail.com Tue Jul 7 11:29:43 2015 From: sprice2003 at gmail.com (Sally Price) Date: Tue, 7 Jul 2015 12:29:43 -0400 Subject: [Histonet] 12ml tubes for Dako IHC stainers Message-ID: Can anyone recommend a vendor for the 12ml tubes that are used on original Dako stainers other than Dako? -- Sally Price From Behnaz.Sohrab at ah.org Tue Jul 7 12:10:10 2015 From: Behnaz.Sohrab at ah.org (Sohrab,Behnaz) Date: Tue, 7 Jul 2015 17:10:10 +0000 Subject: [Histonet] Protocol? Message-ID: <92096F092E8CE54FBB5E272198FE5A7E029D2424@ahexcms001.ah.org> Wondering what is the Protocol for Temporal Artery IN your LAB? Behnaz Sohrab Regional Manager Anatomic pathology Behnaz.Sohrab at ah.org 323-268-5000 Ext.1711 323-265-5086 FAX From areichard at lmhealth.org Tue Jul 7 12:17:34 2015 From: areichard at lmhealth.org (Amanda Reichard) Date: Tue, 7 Jul 2015 13:17:34 -0400 Subject: [Histonet] Protocol? In-Reply-To: <92096F092E8CE54FBB5E272198FE5A7E029D2424@ahexcms001.ah.org> References: <92096F092E8CE54FBB5E272198FE5A7E029D2424@ahexcms001.ah.org> Message-ID: 9 levels with 2 elastic stains after level 3 and level 6 Amanda Reichard, B.S.S., HTL (ASCP)cm Histology Supervisor Laboratory Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (740) 348-4157 ________________________________________ From: Sohrab,Behnaz [Behnaz.Sohrab at ah.org] Sent: Tuesday, July 07, 2015 1:10 PM To: 'Histonet at lists.utsouthwestern.edu' Subject: [Histonet] Protocol? Wondering what is the Protocol for Temporal Artery IN your LAB? Behnaz Sohrab Regional Manager Anatomic pathology Behnaz.Sohrab at ah.org 323-268-5000 Ext.1711 323-265-5086 FAX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From lcolbert at pathmdlabs.com Tue Jul 7 12:36:45 2015 From: lcolbert at pathmdlabs.com (Laurie Colbert) Date: Tue, 7 Jul 2015 17:36:45 +0000 Subject: [Histonet] Protocol? In-Reply-To: <92096F092E8CE54FBB5E272198FE5A7E029D2424@ahexcms001.ah.org> References: <92096F092E8CE54FBB5E272198FE5A7E029D2424@ahexcms001.ah.org> Message-ID: <12ECD7346266D74691EC2BFC75285E456DA22212@BFL323E10.pathmdlabs.local> Nine slides with 3 levels each, trichrome and elastic stain. Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: Sohrab,Behnaz [mailto:Behnaz.Sohrab at ah.org] Sent: Tuesday, July 07, 2015 10:10 AM To: 'Histonet at lists.utsouthwestern.edu' Subject: [Histonet] Protocol? Wondering what is the Protocol for Temporal Artery IN your LAB? Behnaz Sohrab Regional Manager Anatomic pathology Behnaz.Sohrab at ah.org 323-268-5000 Ext.1711 323-265-5086 FAX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From afoshey at mcw.edu Tue Jul 7 12:52:42 2015 From: afoshey at mcw.edu (Foshey, Annette) Date: Tue, 7 Jul 2015 17:52:42 +0000 Subject: [Histonet] Specimens lost during processing. In-Reply-To: References: Message-ID: <3A3991C73D92534BA68571EE65063158C3AC15@MCWMB9.mcwcorp.net> In reference to the first point - we had a grossing log and matched what was written on the log by the P.A. or pathologist to what was actually in the processing baskets that way the person setting up the processors knew right away if something was written incorrectly on the log or what other troubleshooting you had to perform. -----Original Message----- From: STEVEN PINHEIRO [mailto:SPINHEIRO at lumc.edu] Sent: Monday, July 06, 2015 1:28 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Specimens lost during processing. All, Looking for help in analyzing the entire scope of the process. There is not much published data (that I can find) and I am hoping this group can lend some expertise. Our rate is higher than we would like it to be. There is no consistent size at risk although GI and Derm biopsies are the biggest involved group. We have broken it down into steps. 1. Can be lost at grossing- either never loaded into the cassette at all, or cassette was discarded. Thus we hold on to our waste and can search for misdirected cassettes if need be. 2. Lost in the processor itself. Most are wrapped. If large enough not to be wrapped, we would not expect the processor to eat them, so assume cassette lid not properly closed. Frankly the highest number of losses we're seeing is no tissue found in cassette by embedders. 3. I am being told that we can't use micromesh cassettes in our microwave processors (Milestone Pathos) and want to know if anyone is. 4. Tissue not seen at embedding. Again no way to tell when the tissue disappeared. We know that tiny tissue can spring out during the opening at embedding but I don't know how else to examine or limit this step. 5. Tissue can be exhausted during microtomy. Rare but noteworthy. I am hoping people can tell me about their procedures for dealing with "specimens that don't survive processing", what safeguards they have in place, and to some extent what your own lab percentage or experience is. Apologies in advance for the length of the message, but could really use your help. Steven Pinheiro, MBA, MLS(ASCP)DLMCM Manager Anatomic Pathology and Cytology Loyola University Medical Center 2160 S First Ave, Bldg 110 Rm 2214 Maywood, IL 60153 708-327-2642 (O) 708-327-2620 (F) spinheiro at lumc.edu "You must do the thing you think you cannot do" E. Roosevelt Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From bennett777 at gmail.com Tue Jul 7 13:06:29 2015 From: bennett777 at gmail.com (Kevin Bennett) Date: Tue, 7 Jul 2015 14:06:29 -0400 Subject: [Histonet] Histonet Digest, Vol 140, Issue 5 In-Reply-To: References: Message-ID: Alkaline Phosphatse bubbles Hi Tim, Use Dako ultramount. The bubbles are caused by the naphthyl breaking down and releasing CO2 under the coverslip. Apply small amount of the ultramount to cover the muscle tissue and bake in 65 degree oven of 15 to 20 minutes. If you bake it too long the myofibers will draw up and damage the morphology of the section. Kevin On Jul 7, 2015 1:29 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Specimens lost during processing. (STEVEN PINHEIRO) > 2. Preventing Bubbles in Alkaline Phosphatase (Morken, Timothy) > 3. Histology on Rat spine. (Clough, Bret) > 4. 12ml tubes for Dako IHC stainers (Sally Price) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 6 Jul 2015 18:28:21 +0000 > From: STEVEN PINHEIRO > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Specimens lost during processing. > Message-ID: > < > B06172B2B6AB254DB7DA4F8DBF110042801D69F3 at SB01MSTMBX01.sb.trinity-health.org > > > > Content-Type: text/plain; charset="us-ascii" > > All, > Looking for help in analyzing the entire scope of the process. There is > not much published data (that I can find) and I am hoping this group can > lend some expertise. > Our rate is higher than we would like it to be. There is no consistent > size at risk although GI and Derm biopsies are the biggest involved group. > We have broken it down into steps. > > 1. Can be lost at grossing- either never loaded into the cassette at > all, or cassette was discarded. Thus we hold on to our waste and can search > for misdirected cassettes if need be. > > 2. Lost in the processor itself. Most are wrapped. If large enough > not to be wrapped, we would not expect the processor to eat them, so assume > cassette lid not properly closed. Frankly the highest number of losses > we're seeing is no tissue found in cassette by embedders. > > 3. I am being told that we can't use micromesh cassettes in our > microwave processors (Milestone Pathos) and want to know if anyone is. > > 4. Tissue not seen at embedding. Again no way to tell when the > tissue disappeared. We know that tiny tissue can spring out during the > opening at embedding but I don't know how else to examine or limit this > step. > > 5. Tissue can be exhausted during microtomy. Rare but noteworthy. > I am hoping people can tell me about their procedures for dealing with > "specimens that don't survive processing", what safeguards they have in > place, and to some extent what your own lab percentage or experience is. > Apologies in advance for the length of the message, but could really use > your help. > > > Steven Pinheiro, MBA, MLS(ASCP)DLMCM > Manager Anatomic Pathology and Cytology > Loyola University Medical Center > 2160 S First Ave, Bldg 110 Rm 2214 > Maywood, IL 60153 > 708-327-2642 (O) > 708-327-2620 (F) > spinheiro at lumc.edu > > "You must do the thing you think you cannot do" > E. Roosevelt > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > > > ------------------------------ > > Message: 2 > Date: Mon, 6 Jul 2015 19:39:35 +0000 > From: "Morken, Timothy" > To: Histonet > Subject: [Histonet] Preventing Bubbles in Alkaline Phosphatase > Message-ID: > <761E2B5697F795489C8710BCC72141FF602F63AC at ex07.net.ucsf.edu> > Content-Type: text/plain; charset="iso-8859-1" > > All knowing Histonet.... > > We started doing alkaline phosphatase on muscle frozen a little while ago > and have had an issue with apparent air bubbles forming over the tissue. > Not trapped air from coverslipping, but forming from apparent reaction in > the tissue. Does anyone have experience with this and a solution? > > Images: > http://histosearch.com/imageupload/alkaline-phosphatase-bubbles/ > > We tried longer formalin fixation after the stain (10 min) and an acetic > acid rinse after the tap water, after the formalin. Still the same problem > > The only mention I have found about this is a Histochemistry text by Lojda > from 1979 that says to fix in formalin for several hours after staining to > prevent bubble formation. Does anyone have anything shorter? I don't really > remember seeing this when I did these stains 20 years ago.... > > > > Our procedure (from a method given to our neuropath folks by a group in > Australia): > > Frozen sections of muscle > > > Borate Buffer, pH 8.8: > 0.992 g Boric Acid > 2.28 g Sodium Tetraborate > 200 ml distilled water > Mix well. Adjust to pH 8.8. Store at 4?C. > > 0.1M Magnesium Sulphate > 0.6 g Magnesium sulphate, anhydrous (M7506-500G) > 50 ml distilled water > Store at 4?C. > > ALP Incubation Solution: > 15 ml Borate Buffer > 2 ml 0.1M Magnesium Sulphate > 16.5 mg 1-naphthyl phosphate > 16.5 mg Fast Blue RR > Mix in well order. Filter. > > Glycerin Jelly Mounting Media > > > 1. Place glycerin jelly in 60?C oven to liquify > 2. Air dry slides 15 minutes. > 3. Incubate in filtered ALP Incubation Solution at 37?C...45 minutes. > 4. Rinse in tap water. > 5. Change to 10% formalin...1 min > 6. Rinse in tap water. > 7. Air dry. > 8. Coverslip with Glycerin Jelly. > > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > 505 Parnassus Ave, Box 1656 > Room S570 > San Francisco, CA 94143 > > (415) 353-1266 (ph) > (415) 514-3403 (fax) > tim.morken at ucsfmedctr.org > > > > ------------------------------ > > Message: 3 > Date: Tue, 7 Jul 2015 03:05:14 +0000 > From: "Clough, Bret" > To: "Histonet list serv. (histonet at lists.utsouthwestern.edu)" > > Subject: [Histonet] Histology on Rat spine. > Message-ID: > <28529330310DFE4A9F6FFF708AEB090C552CC8CA at CSR-Mail1.ad.tamhsc.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Hello everyone, > > > > I have some rat spines that I need to decal, process, and embed for > sectioning. Is there someone in the histonet community that would be > willing to either share with me their protocol or at least give me guidance > with these spines. These are larger than any tissue that I have ever had to > process. The spines have been fixed in Carson's fixative. Any help would > greatly be appreciated. > > > > Sincerely, > > Bret Clough > > Texas A&M Health Science Center > > Temple, Texas > > > ------------------------------ > > Message: 4 > Date: Tue, 7 Jul 2015 12:29:43 -0400 > From: Sally Price > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] 12ml tubes for Dako IHC stainers > Message-ID: > < > CAHonC4Bnfypr5XUL3o92B3VtTjACKrbRPBLs5YcGSkOV9GsGUg at mail.gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Can anyone recommend a vendor for the 12ml tubes that are used on original > Dako stainers other than Dako? > > > > -- > Sally Price > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 140, Issue 5 > **************************************** > From SteveM at mcclainlab.com Tue Jul 7 13:06:42 2015 From: SteveM at mcclainlab.com (Steve McClain) Date: Tue, 7 Jul 2015 18:06:42 +0000 Subject: [Histonet] Histonet Digest, Vol 140, Issue 5 specimens lost Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DFBFECF73C@ML1.McClainLabs.local> Steven, The problems associated with tissues lost are quite similar to those with floaters. Here is a newly edited copy of what I posted a few years ago on floaters and you may find it useful. We still wrap nearly all specimens. At times we cannot see minute flakes of tissue, but retrieve them by gently scraping the inside of the paper wrap. Floaters on the water bath are generally not the problem, are usually identifiable, unlike the serious issue posed by floaters in the block. I would suggest counting floaters to get a benchmark- ANY COUNT YOU MAKE WILL PROBABLY BE AN UNDERESTIMATE, BUT AT LEAST YOU HAVE SOMETHING TO TRACK.. In order to change the outcome, you (the institutional you) must change what you are doing. SO start by first examining and then changing your grossing-procedure. Start by scrupulously cleaning every pair of forceps. Keep an open vial of 50% alcohol at the bench to have the grossers rinse their forceps between specimens. Photograph every specimen-this forces the grossers to maintain a clean background. If you do not have grossing cameras, then mount a video camera above the grossing station and let it run. Point is a few hours study of technique can help identify where the problem is. Better than wasting many hours. Have grossers begin to wrap every small specimen in lens paper- Four darn good reasons. Wrapping specimens with forceps helps to clean the forceps before the next specimen. At embedding , the action of opening/unwrapping specimens with forceps cleans forceps at the embedding center. Floaters can also occur during processing-wrapping specimens prevents floaters during processing and keeps processors clean. (If you don't believe me- filter your first reagent next time you change it. Then process that to a block- I once inherited a processor from a lab that did placentas. We found pieces of placenta in our derm specimens as long as 5 or 6 months later.) Fourth, even if the specimen lid opens, the tissue is usually still inside the wrapper and is not lost. We bag and save our wrappers until the cases are signed out, but rarely have we had to go hunting. Change fixative and clean block bucket at the grossing bench routinely. You may wish to consider rinsing all blocks in fresh reagent BEFORE placing on processor. As we evolved to this procedure, the floaters fell to 1 per 15,000, about 3 per year in our small lab. ADDITIONAL NOTES: Focusing on grossing is neither putting all your eggs in one basket nor blaming the grossers. Focusing on grossing floaters is focusing on the complex, dirty workplace where historically the vast majority (70-80-90%) of these errors are generated. The people may be part of the problem, yet the system is at the heart of the issue and the system determines the error-rate. The system is complicated, but includes the environment and structure and policy. There is too much going on at or near the grossing bench. Make it quiet. Make it simple. Reduce distractions. There are generally more grossers than embedders and greater variation in technique and sample size-(embedders generally only have to deal with samples 15x15mm or less.) And greater numbers of reagents and fixatives And perhaps even a cryostat for FS Or FAXITRON. Or a computer Accessioning, scanning documents and cassette printing. Maybe a telephone. Or a reagent recycler Or a processor (eliminate as many distractions as you can. Separate quiet enclosed areas where specimens can be controlled and studied without distraction. For example we banned cellphones from the grossing area). Point is simplify and reduce noise. Make the grossing area for small specimens really quiet. The singularly important reason we never adopted voice recognition in the grossing area had to do with the distractions imposed by messing with computers, the noise of talking into a machine, the distraction of looking at a screen to proofread while working with a one of a kind valuable specimen in the most error-prone area of the lab. Talking and looking at words on a computer screen is neither studying tissue nor professional. If you use that type of system, consider modifying your procedure to focus on getting the tissue cleanly in the block and the block safely in reagent, then do the dictation. Some of our grossers were resistant to change with valid reasons for doing so we let them be the baseline control- when we wanted to make a change we compared method A to method B. When one method was statistically better we showed them the data. There is room for the inevitable disagreement- but study and get some data and restudy where needed. Personally, I want to see grosser focused on the tissue, studying tissue as a trained professional; Once the tissue is safely in the cassette and in the next reagent (or fixative or ) then the grossers enter their own data. Scan the next bottle barcode and move on to the next specimen. 'Clean' is a relative term and a perceptual problem. (What is clean?-even when your grossing area is spotless-if one were to eat in the lab would you prefer to do so standing next to the grosser or the embedder? Not suggesting it is permitted- just which area is cleaner and what is meant by clean) SUGGESTIONS Have an EM (electron microscope) tech come in and get it clean to his/her satisfaction. They are used to clean-room mentality and standards. Sometime Grossers are macho or otherwise reluctant to change paper towels or other cutting surfaces or cutting tools between specimens. Make the tools and towels freely available. Make the grossing benches spotless. Make the reagent buckets, spotless. Fresh reagents every shift- or whenever a speck appears. The instruments, spotless. Cut white or blue flat paper towels into 2.5x2.5 inch squares and gross on them- fewer floaters than brown C-fold towels or use pink dental wax sheets. Make a hard and fast rule about the number of tissue fragments per cassette, e.g., 5- not more. 5 pieces in a cassette can be seen and verified easily. Place similar sized pieces in cassettes (if there are 10 pieces, 5 each 1mm and 5 each 3mm- all the 1mm go into one cassette and all the 3 mm in the other cassette. A 1mm speck-floater is not obvious among 3mm pieces. A sixth 1mm speck in the 1mm cassette is noticeable. Use a separate area to gross large specimens and small biopsies, whether on different benches or at different times of day than the small biopsies. ADVANTAGES OF PHOTOGRAPHY When one photographs every specimen as we do at McClain Labs and critiques grossers for knifemarks or bloody stains on the towel or leaving the same bloody background in consecutive photos by posting them in the lab or at staff meetings, they soon catch on. Rewards for great photographs also sends a message. Photography is a great method for verifying technique, for documenting your work, but it does slow us down. 11 years later, we still photograph every specimen. METRICS- fewer is better. Track floaters or lost specimens by grosser to see if you have an outlier or risk-taker. Performance improves on any measured parameter when one follows through. Historically grossing performance has been measured by how fast the grosser completes x number of specimens. That metric should be suspended or abandoned if there are issues in the lab or while you are working toward improving technique. FYI our lab has very few metrics. Our main metrics are 1) lost specimens- (the number was zero in seven years and is now 1 in 11 years) 2) cumulative blocks since last maintenance for each processor. 3) catastrophic tissue loss- sounds a bit extreme, but is defined as any hole or missing tissue on the slide visible at low power (2x). I photograph each such slide, investigate and generally find maintainance on the processors. These we discuss at staff meeting of grossers and histotechs and pathologists. PERSONNEL Training histotechs to gross has a variety of benefits. My good experience biases me -my best grossers have been histotechs. They respect the difficulty in embedding and cutting and communicate well with other histotechs. My second best are pathologists (I am the slowest) Physician assistants and residents are difficult (or maybe I demand too much). Both operate largely outside traditional lab feedback loops. (Feedback to residents may amount to being castigated by the attending who is legally responsible for their mistakes at the microscope and feeling discomfort. But neither residents nor PA's have to embed or cut their mistakes at the microtome, which falls to the histotechs) I'd rather gross myself than to clean up messes made by residents. I was a jerk resident myself 1000 years ago- you know the trainee who is told to keep the blocks postage stamp-size and brings in a commemorative stamp? The process for eliminating floaters or lost tissues is analogous to troubleshooting staining problems. Look to the early steps because errors in technique in the early steps cause great variation in the final product. Begin by eliminating early points of failure (for staining-first care in handling prior to fixation, followed by prompt fixation, adequate fixation, then consistent thickness in grossing, then processing, then cutting). Errors in early steps cannot be fixed later on. Mashing tissue or prolonged drying or inadequate fixation cannot be fixed by re-processing. Once the floater is in the block, it is there to stay and proof by testing the DNA of the floater and patient is expensive, used to be $3000-$5000. It is better and less expensive to eliminate these errors at the source. Focusing on grossing not only gets rid of the main source(s) of variation, it also serves notice to the embedders once "grossing floaters and "processing floaters" are eliminated embedding is next. Steve A. McClain, MD 631 361 4000 631 361 4000 -----Original Message----- 1. Specimens lost during processing. (STEVEN PINHEIRO) From tbraud at holyredeemer.com Tue Jul 7 13:24:42 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 7 Jul 2015 18:24:42 +0000 Subject: [Histonet] Histonet Digest, Vol 140, Issue 5 In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F801ED8D@HRHEX02-HOS.holyredeemer.local> In the article reference below, they estimate tissue loss at 7%. In my lab, our pathologists would croak if we had tissue loss at this high of a rate. But with that said, we do have a 0.8% "loss of tissue". Without exception, they have been extremely small pieces that could have been mucous, and they have been frequently described as "possibly too small to survive processing". We've had a cassette discarded during processing twice in 10 years, however we save all trash until the tissue has been embedded and the piece count and block log verified. In both cases, we were able to go back to the gross station trash and retrieve the cassette. We use blue pads to hold bx tissue in the cassette but the pieces are placed on the pad as if on a grid. One piece: direct center Two pieces: [:] Three pieces: [:.] Four pieces: [::] and so on. If they are wrapped in lens paper, we use a special folding technique and write a target on the paper and place the tissue on the bulls eye. Large sections of skin are held in place with a single blue pad and are always placed in the cassette the same way at gross. For a bisected punch, [:] For a serial sectioned ellipse submitted in one cassette, it is tip, x-sect, x-sect, tip [.//.] For a multicassette skin ellipse, it is both tips in the first cassette, and x-sections in the following serial cassettes. If it's there, then the grosser should have communicated to the tech, "may not survive processing" or "very tiny" on the gross worksheet notes. Often, the gross person will have another set of eyes look at a questionable piece, if they think it may not survive processing. We hold techs accountable to match the piece count and it is inexcusable to lose a section of skin at embedding or cut through a specimen. If a pathologist orders recuts where tissue might be cut away, then the tech must notify the pathologist before proceding and a note is placed in the computer that the tissue will be cut away. We hold all papers or blue pads of missing specimens until the gross dictation is reviewed and the pathologist notified. With special care at grossing, we seldom have an incident because the embedders know exactly where to find the tissue and if the tissue is possibly mucous or a spec, that may not survive processing, then it usually is noted at gross and is reported as "tissue not sufficient for processing" with a suggestion to repeat biopsy based on clinical findings. Reference: Owens, SR.; Wiehagen, L.; Simmons, C.; Sikorova, A.; Stewart, W.; Kelly, S.; Nestler, R.; Yousem, SA. (Dec 2011). "Numerical fidelity of endoscopic biopsy fragments in the processing sequence of a university surgical pathology laboratory.". Arch Pathol Lab Med 135 (12): 1561-4. doi:10.5858/arpa.2011-0020-OA. PMID 22129184. Sorry to be so long winded, but I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 -----Original Message----- 1. Specimens lost during processing. (STEVEN PINHEIRO) Message: 1 Date: Mon, 6 Jul 2015 18:28:21 +0000 From: STEVEN PINHEIRO All, Looking for help in analyzing the entire scope of the process. There is not much published data (that I can find) and I am hoping this group can lend some expertise. Our rate is higher than we would like it to be. There is no consistent size at risk although GI and Derm biopsies are the biggest involved group. We have broken it down into steps. 1. Can be lost at grossing- either never loaded into the cassette at all, or cassette was discarded. Thus we hold on to our waste and can search for misdirected cassettes if need be. 2. Lost in the processor itself. Most are wrapped. If large enough not to be wrapped, we would not expect the processor to eat them, so assume cassette lid not properly closed. Frankly the highest number of losses we're seeing is no tissue found in cassette by embedders. 3. I am being told that we can't use micromesh cassettes in our microwave processors (Milestone Pathos) and want to know if anyone is. 4. Tissue not seen at embedding. Again no way to tell when the tissue disappeared. We know that tiny tissue can spring out during the opening at embedding but I don't know how else to examine or limit this step. 5. Tissue can be exhausted during microtomy. Rare but noteworthy. I am hoping people can tell me about their procedures for dealing with "specimens that don't survive processing", what safeguards they have in place, and to some extent what your own lab percentage or experience is. Apologies in advance for the length of the message, but could really use your help. Steven Pinheiro, MBA, MLS(ASCP)DLMCM Manager Anatomic Pathology and Cytology Loyola University Medical Center 2160 S First Ave, Bldg 110 Rm 2214 Maywood, IL 60153 708-327-2642 (O) 708-327-2620 (F) spinheiro at lumc.edu "You must do the thing you think you cannot do" E. Roosevelt From simmca at UPMC.EDU Tue Jul 7 14:13:21 2015 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Tue, 7 Jul 2015 19:13:21 +0000 Subject: [Histonet] Histonet Digest, Vol 140, Issue 5 In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F801ED8D@HRHEX02-HOS.holyredeemer.local> References: , <48E053DDF6CE074DB6A7414BA05403F801ED8D@HRHEX02-HOS.holyredeemer.local> Message-ID: <52917B7A-CBA9-48E8-85C9-4BE7C98270CA@UPMC.EDU> Thanks for citing the article I helped participate in! Sent from my iPhone > On Jul 7, 2015, at 2:25 PM, Terri Braud wrote: > > > In the article reference below, they estimate tissue loss at 7%. In my lab, our pathologists would croak if we had tissue loss at this high of a rate. But with that said, we do have a 0.8% "loss of tissue". Without exception, they have been extremely small pieces that could have been mucous, and they have been frequently described as "possibly too small to survive processing". > We've had a cassette discarded during processing twice in 10 years, however we save all trash until the tissue has been embedded and the piece count and block log verified. In both cases, we were able to go back to the gross station trash and retrieve the cassette. > We use blue pads to hold bx tissue in the cassette but the pieces are placed on the pad as if on a grid. > One piece: direct center > Two pieces: [:] > Three pieces: [:.] > Four pieces: [::] and so on. > If they are wrapped in lens paper, we use a special folding technique and write a target on the paper and place the tissue on the bulls eye. > Large sections of skin are held in place with a single blue pad and are always placed in the cassette the same way at gross. > For a bisected punch, [:] > For a serial sectioned ellipse submitted in one cassette, it is tip, x-sect, x-sect, tip [.//.] > For a multicassette skin ellipse, it is both tips in the first cassette, and x-sections in the following serial cassettes. > If it's there, then the grosser should have communicated to the tech, "may not survive processing" or "very tiny" on the gross worksheet notes. Often, the gross person will have another set of eyes look at a questionable piece, if they think it may not survive processing. > We hold techs accountable to match the piece count and it is inexcusable to lose a section of skin at embedding or cut through a specimen. > If a pathologist orders recuts where tissue might be cut away, then the tech must notify the pathologist before proceding and a note is placed in the computer that the tissue will be cut away. > We hold all papers or blue pads of missing specimens until the gross dictation is reviewed and the pathologist notified. > With special care at grossing, we seldom have an incident because the embedders know exactly where to find the tissue and if the tissue is possibly mucous or a spec, that may not survive processing, then it usually is noted at gross and is reported as "tissue not sufficient for processing" with a suggestion to repeat biopsy based on clinical findings. > Reference: > Owens, SR.; Wiehagen, L.; Simmons, C.; Sikorova, A.; Stewart, W.; Kelly, S.; Nestler, R.; Yousem, SA. (Dec 2011). "Numerical fidelity of endoscopic biopsy fragments in the processing sequence of a university surgical pathology laboratory.". Arch Pathol Lab Med 135 (12): 1561-4. doi:10.5858/arpa.2011-0020-OA. PMID 22129184. > Sorry to be so long winded, but I hope this helps. > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Holy Redeemer Hospital Laboratory > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3676 > Fax: 215-938-3874 > > -----Original Message----- > > 1. Specimens lost during processing. (STEVEN PINHEIRO) > Message: 1 > Date: Mon, 6 Jul 2015 18:28:21 +0000 > From: STEVEN PINHEIRO > All, > Looking for help in analyzing the entire scope of the process. There is not much published data (that I can find) and I am hoping this group can lend some expertise. > Our rate is higher than we would like it to be. There is no consistent size at risk although GI and Derm biopsies are the biggest involved group. We have broken it down into steps. > > 1. Can be lost at grossing- either never loaded into the cassette at all, or cassette was discarded. Thus we hold on to our waste and can search for misdirected cassettes if need be. > > 2. Lost in the processor itself. Most are wrapped. If large enough not to be wrapped, we would not expect the processor to eat them, so assume cassette lid not properly closed. Frankly the highest number of losses we're seeing is no tissue found in cassette by embedders. > > 3. I am being told that we can't use micromesh cassettes in our microwave processors (Milestone Pathos) and want to know if anyone is. > > 4. Tissue not seen at embedding. Again no way to tell when the tissue disappeared. We know that tiny tissue can spring out during the opening at embedding but I don't know how else to examine or limit this step. > > 5. Tissue can be exhausted during microtomy. Rare but noteworthy. > I am hoping people can tell me about their procedures for dealing with "specimens that don't survive processing", what safeguards they have in place, and to some extent what your own lab percentage or experience is. > Apologies in advance for the length of the message, but could really use your help. > > > Steven Pinheiro, MBA, MLS(ASCP)DLMCM > Manager Anatomic Pathology and Cytology > Loyola University Medical Center > 2160 S First Ave, Bldg 110 Rm 2214 > Maywood, IL 60153 > 708-327-2642 (O) > 708-327-2620 (F) > spinheiro at lumc.edu > > "You must do the thing you think you cannot do" > E. Roosevelt > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lwottinger at namsa.com Wed Jul 8 06:46:59 2015 From: lwottinger at namsa.com (Laura Westergaard Ottinger) Date: Wed, 8 Jul 2015 11:46:59 +0000 Subject: [Histonet] Long Term Tissue Storage Message-ID: Hello, Are there any articles concerning the optimal conditions for long term storage of tissues in 10%NBF when antigenicity (IHC will not be performed on these tissues) is not a concern? Thanks From Miller.Wendy at mhsil.com Wed Jul 8 08:09:00 2015 From: Miller.Wendy at mhsil.com (Miller, Wendy) Date: Wed, 8 Jul 2015 08:09:00 -0500 Subject: [Histonet] Leica bond Message-ID: <358180E0-07E0-4281-933B-51022B2A8896@mimectl> Hi! Does anyone out there use the Leica IHC Bond stainer? We are looking into purchasing one and I would like to input. Do you like it? Pros and cons? Thanks ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From sheryl.stephenson at ag.state.nj.us Wed Jul 8 08:24:09 2015 From: sheryl.stephenson at ag.state.nj.us (Stephenson, Sheryl) Date: Wed, 8 Jul 2015 13:24:09 +0000 Subject: [Histonet] Leica bond In-Reply-To: <358180E0-07E0-4281-933B-51022B2A8896@mimectl> References: <358180E0-07E0-4281-933B-51022B2A8896@mimectl> Message-ID: So far so good. Been using it for a year now. No issues or grips that couldn't be worked out with Leica. They have been very supportive. Their field staff comes to assist you from start to finish if need be. Leica even has classes to train on their machine in IL; all expenses paid. Oh one thing, their annual PM costs is ridiculously high. Sheryl Stephenson Histotechnologist NJ Department of Agriculture Animal Health Diagnostic Labs -----Original Message----- From: Miller, Wendy [mailto:Miller.Wendy at mhsil.com] Sent: Wednesday, July 08, 2015 9:09 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Leica bond Hi! Does anyone out there use the Leica IHC Bond stainer? We are looking into purchasing one and I would like to input. Do you like it? Pros and cons? Thanks ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed Jul 8 08:36:16 2015 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 8 Jul 2015 09:36:16 -0400 Subject: [Histonet] RELIA Solutions Weekly Histology HOT Job Alert! 7-8-2015 Message-ID: <000501d0b983$10ecc0b0$32c64210$@earthlink.net> Hi Histonetters!! I hope you had a great 4th of July Holiday Weekend!! Things keep heating up around here as the phones continue to ring off the hook with exciting new opportunities!!! The clients are ready to hire ASAP and are offering full time permanent positions with excellent compensation packages, relocation assistance and sign on bonuses. My clients will work around your vacations!! Here is a list of my current openings: IHC Supervisor - Long Island, NY Lead IHC Tech - Waynesboro, VA Histotechnician - Norfolk, VA 1st and 2nd shift 15K sign on bonus Lead Histotechnologist - Great Falls, MT Histology Tech - Asheville, NC HT/HTL - Atlanta, GA HT/HTL - Bellevue, WA More positions are coming in all of the time so if you are looking let me know where and what you are looking for and I will keep you posted. REMEMBER. It never hurts to look!! If you would like more information on any of these positions or about a custom job search let's talk. You can reach me toll free at 866-607-3542 until 6 EST and anytime on my cell phone at 407-353-5070, or shoot me an email at relia1 at earthlink.net to schedule a time to talk. I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a $250.00 referral fee for anyone you refer to me that I place. Have a Great Week!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Joyce.Weems at emoryhealthcare.org Wed Jul 8 08:55:39 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Wed, 8 Jul 2015 13:55:39 +0000 Subject: [Histonet] Leica bond In-Reply-To: <358180E0-07E0-4281-933B-51022B2A8896@mimectl> References: <358180E0-07E0-4281-933B-51022B2A8896@mimectl> Message-ID: We have for several years and are very satisfied with them. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Miller, Wendy [mailto:Miller.Wendy at mhsil.com] Sent: Wednesday, July 08, 2015 9:09 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Leica bond Hi! Does anyone out there use the Leica IHC Bond stainer? We are looking into purchasing one and I would like to input. Do you like it? Pros and cons? Thanks ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From plucas at biopath.org Wed Jul 8 10:27:24 2015 From: plucas at biopath.org (Paula Lucas) Date: Wed, 8 Jul 2015 08:27:24 -0700 Subject: [Histonet] Leica bond In-Reply-To: <358180E0-07E0-4281-933B-51022B2A8896@mimectl> References: <358180E0-07E0-4281-933B-51022B2A8896@mimectl> Message-ID: <85ABEC8CA62A4FC38661B7A70BAAAAE3@biopath.local> Wendy, the Leica Bond is a great machine. We've had it for several years now and it provides excellent and consistent results. Paula Bio-Path Medical Group Fountain Valley, CA -----Original Message----- From: Miller, Wendy [mailto:Miller.Wendy at mhsil.com] Sent: Wednesday, July 08, 2015 6:09 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Leica bond Hi! Does anyone out there use the Leica IHC Bond stainer? We are looking into purchasing one and I would like to input. Do you like it? Pros and cons? Thanks ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DShank at chpnet.org Wed Jul 8 10:16:18 2015 From: DShank at chpnet.org (Deborah Shank) Date: Wed, 8 Jul 2015 11:16:18 -0400 Subject: [Histonet] Leica bond In-Reply-To: <85ABEC8CA62A4FC38661B7A70BAAAAE3@biopath.local> References: <358180E0-07E0-4281-933B-51022B2A8896@mimectl> <85ABEC8CA62A4FC38661B7A70BAAAAE3@biopath.local> Message-ID: We have seven and they are great. Deborah Shank, Manager Immunopathology, Flow Cytometry, Immunohistochemistry, FISH MSBI Ny,NY -----Original Message----- From: Paula Lucas [mailto:plucas at biopath.org] Sent: Wednesday, July 08, 2015 11:27 AM To: 'Miller, Wendy'; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Leica bond Wendy, the Leica Bond is a great machine. We've had it for several years now and it provides excellent and consistent results. Paula Bio-Path Medical Group Fountain Valley, CA -----Original Message----- From: Miller, Wendy [mailto:Miller.Wendy at mhsil.com] Sent: Wednesday, July 08, 2015 6:09 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Leica bond Hi! Does anyone out there use the Leica IHC Bond stainer? We are looking into purchasing one and I would like to input. Do you like it? Pros and cons? Thanks ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message and any attachments are confidential and intended solely for the use of the individual or entity to which they are addressed. If you are not the intended recipient, you are prohibited from printing, copying, forwarding, saving, or otherwise using or relying upon them in any manner. Please notify the sender immediately if you have received this message by mistake and delete it from your system. From Miller.Wendy at mhsil.com Wed Jul 8 10:26:45 2015 From: Miller.Wendy at mhsil.com (Miller, Wendy) Date: Wed, 8 Jul 2015 10:26:45 -0500 Subject: [Histonet] Dako IHC stainer Message-ID: <187D9676-7EAC-494F-80A5-3BB078078C31@mimectl> I recently asked about the Leica Bond IHC stainer, now I'm curious if anyone has the Dako IHC stainer and if you like it? Pros and cons?? Thanks again! ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From rjbuesa at yahoo.com Wed Jul 8 11:08:22 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 8 Jul 2015 16:08:22 +0000 (UTC) Subject: [Histonet] Dako IHC stainer In-Reply-To: <187D9676-7EAC-494F-80A5-3BB078078C31@mimectl> References: <187D9676-7EAC-494F-80A5-3BB078078C31@mimectl> Message-ID: <407745644.1260315.1436371702893.JavaMail.yahoo@mail.yahoo.com> I used the DAKO from 1990 until I retire in 2002 and I like it very much.The Leica Bond is also good and have some newer characteristics, but the DAKO is very "user friendly".Ren? On Wednesday, July 8, 2015 11:42 AM, "Miller, Wendy" wrote: I recently asked about the Leica Bond IHC stainer, now I'm curious if anyone has the Dako IHC stainer and if you like it? Pros and cons?? Thanks again! ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From taylor at prometheushealthcare.com Wed Jul 8 11:48:09 2015 From: taylor at prometheushealthcare.com (Taylor Rinaldi) Date: Wed, 8 Jul 2015 12:48:09 -0400 Subject: [Histonet] Nationwide Histology job opportunities! Message-ID: <035501d0b99d$dfed9dc0$9fc8d940$@prometheushealthcare.com> Hi all! My name is Taylor Rinaldi, Recruiting Manager at Prometheus healthcare. We specialize specifically in laboratory recruiting all over the United States, for different hospitals and reference labs. We just received a new order for a great opportunity near the Seattle, Washington area. A growing Anatomic Pathology laboratory is looking to hire a new Histotechnician for their lab. This position is a fulltime, permanent opportunity offering a lot of growth. ASCP certification preferred. If you may be interested in this or any of our other opportunities, please do not hesitate to reach out to me. Please reach out to me for immediate consideration. Thanks so much! Taylor Rinaldi Recruiting Manager Prometheus Healthcare Office (301) 693-9057 Taylor at prometheushealthcare.com From relia1 at earthlink.net Wed Jul 8 11:57:38 2015 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 8 Jul 2015 12:57:38 -0400 Subject: [Histonet] RELIA HOT HISTOLOGY JOB ALERT HISTOTECHS NEEDED - SEATTLE, WA Message-ID: <00ef01d0b99f$412b4dc0$c381e940$@earthlink.net> Hi Histonetters!! As I said in my previous post my phone continues to ring off the hook with exciting opportunities. A client of ours located in the Seattle area has several night shift histotech positions open. They offer a competitive pay rate relocation assistance and a great shift differential. They have a really nice benefits package and a great team to work with. If you would like more info please contact me. You can reach me via email at relia1 at earthlink.net or toll free at 866-607-3542 or cell or text at 407-353-5070. And if you know someone who might be interested and you refer them to me --- If I place them you earn a referral fee. I love to pay referral fees!! Have a great day!! Hope to hear from you!!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From SteveM at mcclainlab.com Wed Jul 8 12:47:28 2015 From: SteveM at mcclainlab.com (Steve McClain) Date: Wed, 8 Jul 2015 17:47:28 +0000 Subject: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DFBFED2A37@ML1.McClainLabs.local> My goodness 7% tissue loss is ridiculous. What fool published that? A 0.8% "loss of tissue" is beyond me. Would any one advertise in the local paper that your center of excellence lab loses nearly 1% of your specimens? "possibly too small to survive processing" is in my opinion, a self-fulfilling prophesy, one to be eschewed and denounced, or beaten out of the grosser who dares use that description for it is an expectation of failure. That grosser would not last a week around me. I would walk them to the train station and ask them not to return. I felt less cantankerous before I read that one. Steve A. McClain, MD 631 361 4000 **** From lblazek at digestivespecialists.com Wed Jul 8 13:40:27 2015 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Wed, 8 Jul 2015 14:40:27 -0400 Subject: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss In-Reply-To: <012ADA4B5CC00F4AB5E4BAA399E0A5DFBFED2A37@ML1.McClainLabs.local> References: <012ADA4B5CC00F4AB5E4BAA399E0A5DFBFED2A37@ML1.McClainLabs.local> Message-ID: <5A2BD13465E061429D6455C8D6B40E3917440489F2@IBMB7Exchange.digestivespecialists.com> Dear Mr. Cantankerous, :) So how would you gross a colon biopsy that was <0.1 cm and probably mucus? -----Original Message----- From: Steve McClain [mailto:SteveM at mcclainlab.com] Sent: Wednesday, July 08, 2015 1:47 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss My goodness 7% tissue loss is ridiculous. What fool published that? A 0.8% "loss of tissue" is beyond me. Would any one advertise in the local paper that your center of excellence lab loses nearly 1% of your specimens? "possibly too small to survive processing" is in my opinion, a self-fulfilling prophesy, one to be eschewed and denounced, or beaten out of the grosser who dares use that description for it is an expectation of failure. That grosser would not last a week around me. I would walk them to the train station and ask them not to return. I felt less cantankerous before I read that one. Steve A. McClain, MD 631 361 4000 **** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryeo at wchosp.org Wed Jul 8 14:19:02 2015 From: ryeo at wchosp.org (Richard Yeo) Date: Wed, 8 Jul 2015 19:19:02 +0000 Subject: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss In-Reply-To: <012ADA4B5CC00F4AB5E4BAA399E0A5DFBFED2A37@ML1.McClainLabs.local> References: <012ADA4B5CC00F4AB5E4BAA399E0A5DFBFED2A37@ML1.McClainLabs.local> Message-ID: Steve, I totally agree that 7% tissue loss is extremely high. Saying that when you have an experienced pathologist that looks at a specimen and only sees mucoid material he knows that it is most likely that the reagents in the processor are going to dissolve it. We have a very low rate of loss, probably one to two blocks per thousand processed. That's.01-.02%. No one wants to lose any tissue specimen and put the patient through it again but it happens. Rich Y Sent from my iPhone > On Jul 8, 2015, at 1:50 PM, Steve McClain wrote: > > My goodness 7% tissue loss is ridiculous. > What fool published that? > > A 0.8% "loss of tissue" is beyond me. > Would any one advertise in the local paper that your center of excellence lab loses nearly 1% of your specimens? > > "possibly too small to survive processing" is in my opinion, a self-fulfilling prophesy, > one to be eschewed and denounced, > or beaten out of the grosser who dares use that description for it is an expectation of failure. > That grosser would not last a week around me. > I would walk them to the train station and ask them not to return. > > I felt less cantankerous before I read that one. > Steve A. McClain, MD > 631 361 4000 > **** > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kkienitz at orclinic.com Wed Jul 8 15:44:13 2015 From: kkienitz at orclinic.com (Kienitz, Kari) Date: Wed, 8 Jul 2015 13:44:13 -0700 Subject: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss In-Reply-To: <012ADA4B5CC00F4AB5E4BAA399E0A5DFBFED2A37@ML1.McClainLabs.local> References: <012ADA4B5CC00F4AB5E4BAA399E0A5DFBFED2A37@ML1.McClainLabs.local> Message-ID: <41400FFE517878449D89114DD2526090196602D1B4@tocmail1.tocad.orclinic.com> whoa! 7% of specimens? Working in a high volume colonoscopy laboratory for 4 years, I've only had two specimens that were so minute there was nothing at embedding. Bio-wraps or lens paper is a must. Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz at orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: Steve McClain [SteveM at mcclainlab.com] Sent: Wednesday, July 08, 2015 10:47 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss My goodness 7% tissue loss is ridiculous. What fool published that? A 0.8% "loss of tissue" is beyond me. Would any one advertise in the local paper that your center of excellence lab loses nearly 1% of your specimens? "possibly too small to survive processing" is in my opinion, a self-fulfilling prophesy, one to be eschewed and denounced, or beaten out of the grosser who dares use that description for it is an expectation of failure. That grosser would not last a week around me. I would walk them to the train station and ask them not to return. I felt less cantankerous before I read that one. Steve A. McClain, MD 631 361 4000 **** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SPINHEIRO at lumc.edu Wed Jul 8 16:24:01 2015 From: SPINHEIRO at lumc.edu (STEVEN PINHEIRO) Date: Wed, 8 Jul 2015 21:24:01 +0000 Subject: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss In-Reply-To: <41400FFE517878449D89114DD2526090196602D1B4@tocmail1.tocad.orclinic.com> References: <012ADA4B5CC00F4AB5E4BAA399E0A5DFBFED2A37@ML1.McClainLabs.local> <41400FFE517878449D89114DD2526090196602D1B4@tocmail1.tocad.orclinic.com> Message-ID: I was the one that started the inquiry, however SO there is no confusion, my lab is NOT the one with the 7% loss. Steve Pinheiro 708-327-2642 -----Original Message----- From: Kienitz, Kari [mailto:kkienitz at orclinic.com] Sent: Wednesday, July 08, 2015 3:44 PM To: Steve McClain; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss whoa! 7% of specimens? Working in a high volume colonoscopy laboratory for 4 years, I've only had two specimens that were so minute there was nothing at embedding. Bio-wraps or lens paper is a must. Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz at orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: Steve McClain [SteveM at mcclainlab.com] Sent: Wednesday, July 08, 2015 10:47 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss My goodness 7% tissue loss is ridiculous. What fool published that? A 0.8% "loss of tissue" is beyond me. Would any one advertise in the local paper that your center of excellence lab loses nearly 1% of your specimens? "possibly too small to survive processing" is in my opinion, a self-fulfilling prophesy, one to be eschewed and denounced, or beaten out of the grosser who dares use that description for it is an expectation of failure. That grosser would not last a week around me. I would walk them to the train station and ask them not to return. I felt less cantankerous before I read that one. Steve A. McClain, MD 631 361 4000 **** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From hlukey at msn.com Wed Jul 8 19:28:34 2015 From: hlukey at msn.com (Hugh Luk) Date: Wed, 8 Jul 2015 14:28:34 -1000 Subject: [Histonet] 12ml tubes for Dako IHC stainers In-Reply-To: References: Message-ID: Hi Sally, Sarstedt (Germany) used to make these. Cat #62.732.512, but my web inquiry leads to a null field(??). Per Histosearch, distributors used to include Labvision (purchased by Thermo) and Fisher (also purchased by Thermo). My search on Thermo-Fisher shows they still have (excerpt below), but my institutional price is $371 for 100-15ml tubes. Hope your price is less ...traumatic. If your price is also high, Sarstedt's Nevada or North Carolina office might be helpful? [Sarstedt.com, (West Coast Sales) NV-USA, 800-257-5101 (only in the USA), E-Mail: customerservice at sarstedt.us] Anyone else found a 750 mm x 200 mm (hopefully) sterile vial or tube that can be used as a replacement? I am having no luck. Hugh Hawaii ps. not affiliated with any groups above. Just old. > ------------------------------ > > Date: Tue, 7 Jul 2015 12:29:43 -0400 > From: Sally Price > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] 12ml tubes for Dako IHC stainers > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Can anyone recommend a vendor for the 12ml tubes that are used on original > Dako stainers other than Dako? > -- > Sally Price via histosearch Tue Jul 24 09:18:40 CDT 2007 >From Joanne Mauger mauger <@t> email.chop.edu Hazel, We order reagent vials from Labvision through Fisher#S20002. You get 100-15 ml. vials for $40. They are exactly the same as the ones from Dako. Jo From Terry.Dyer at tmmc.com Thu Jul 9 06:13:19 2015 From: Terry.Dyer at tmmc.com (Dyer, Terry) Date: Thu, 9 Jul 2015 11:13:19 +0000 Subject: [Histonet] reply Message-ID: <52B7E4673B4A6045988BC7065CA3B84815AD7928@EXCH-2.tmmc.com> Replying to confirmation Thank you! From SteveM at mcclainlab.com Thu Jul 9 06:52:35 2015 From: SteveM at mcclainlab.com (Steve McClain) Date: Thu, 9 Jul 2015 11:52:35 +0000 Subject: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss In-Reply-To: <5A2BD13465E061429D6455C8D6B40E3917440489F2@IBMB7Exchange.digestivespecialists.com> References: <012ADA4B5CC00F4AB5E4BAA399E0A5DFBFED2A37@ML1.McClainLabs.local> <5A2BD13465E061429D6455C8D6B40E3917440489F2@IBMB7Exchange.digestivespecialists.com> Message-ID: <012ADA4B5CC00F4AB5E4BAA399E0A5DFBFED471A@ML1.McClainLabs.local> Ms. Blazek, To clarify the matter, I read the cited paper by SR Owens, and I interpret those data differently, and in my opinion, the 7% figure for "lost" specimens is incorrectly quoted. The majority of his cases where there was a difference between the number of fragments submitted and the fragments observed on the slide involved a gain in number due to fragmentation in 5% of cases. Where tissue was "lost" (0.6% had fewer fragments at embedding and 6% had fewer on the slide) that mainly appeared to occur during the microtomy phase, due to exhaustion of the fragment in the block. Owens gives no indication there were any cases where all tissue was lost. Nor does he specifically define the minimum size to be counted as a fragment, e.g., how small is a fragment. These data do not support the daily loss of all fragments in a specimen block or the expectation that tissue will not survive processing. In UPMC protocol They wrap all specimens, ink the tissue with eosin (erythrosine) at the grossing bench and cut two levels at 40 microns. Steve A. McClain, MD 631 361 4000 -----Original Message----- From: Blazek, Linda [mailto:lblazek at digestivespecialists.com] Sent: Wednesday, July 08, 2015 2:40 PM To: Steve McClain; histonet at lists.utsouthwestern.edu Subject: RE: Histonet Digest, Vol 140, Issue 6 Specimen loss Dear Mr. Cantankerous, :) So how would you gross a colon biopsy that was <0.1 cm and probably mucus? -----Original Message----- From: Steve McClain [mailto:SteveM at mcclainlab.com] Sent: Wednesday, July 08, 2015 1:47 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss My goodness 7% tissue loss is ridiculous. What fool published that? A 0.8% "loss of tissue" is beyond me. Would any one advertise in the local paper that your center of excellence lab loses nearly 1% of your specimens? "possibly too small to survive processing" is in my opinion, a self-fulfilling prophesy, one to be eschewed and denounced, or beaten out of the grosser who dares use that description for it is an expectation of failure. That grosser would not last a week around me. I would walk them to the train station and ask them not to return. I felt less cantankerous before I read that one. Steve A. McClain, MD 631 361 4000 **** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From simmca at UPMC.EDU Thu Jul 9 07:02:32 2015 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Thu, 9 Jul 2015 12:02:32 +0000 Subject: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss In-Reply-To: <012ADA4B5CC00F4AB5E4BAA399E0A5DFBFED471A@ML1.McClainLabs.local> References: <012ADA4B5CC00F4AB5E4BAA399E0A5DFBFED2A37@ML1.McClainLabs.local> <5A2BD13465E061429D6455C8D6B40E3917440489F2@IBMB7Exchange.digestivespecialists.com> <012ADA4B5CC00F4AB5E4BAA399E0A5DFBFED471A@ML1.McClainLabs.local> Message-ID: <6295CCF61B0DBB4C9248F51F8940E6A1011FCECF@MSXMBXNSPRD09.acct.upmchs.net> As one of the lead participants in the cited study.... We never "lost" specimens. Did we have fewer than was noted on the casette from gross, yes. Could we attribute that to mucoid samples, yes and no. We counted everything, even dust like particles, hence the 6% fewer on the slide. Keeping in mind that a full face is needed for any diagnosis, these particles were exhausted from the block achieving this. We also kept track of those specimens that either fragmented during processing or when they were handled by the embedding technicians. WE DO NOT LOSE 7% OF OUR TISSUE. The protocol is to wrap 0.2-0.3cm and below in biopsy paper. This makes more work for the grossers/embedders, but it is better than fishing samples apart from the ball in the corner they become during processing if you don't. Now that we are clear (hopefully) can we drop this pointless thread? Chris Simmons B.S., A.S., HTL(ASCP) -----Original Message----- From: Steve McClain [mailto:SteveM at mcclainlab.com] Sent: Thursday, July 09, 2015 7:53 AM To: Blazek, Linda; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss Ms. Blazek, To clarify the matter, I read the cited paper by SR Owens, and I interpret those data differently, and in my opinion, the 7% figure for "lost" specimens is incorrectly quoted. The majority of his cases where there was a difference between the number of fragments submitted and the fragments observed on the slide involved a gain in number due to fragmentation in 5% of cases. Where tissue was "lost" (0.6% had fewer fragments at embedding and 6% had fewer on the slide) that mainly appeared to occur during the microtomy phase, due to exhaustion of the fragment in the block. Owens gives no indication there were any cases where all tissue was lost. Nor does he specifically define the minimum size to be counted as a fragment, e.g., how small is a fragment. These data do not support the daily loss of all fragments in a specimen block or the expectation that tissue will not survive processing. In UPMC protocol They wrap all specimens, ink the tissue with eosin (erythrosine) at the grossing bench and cut two levels at 40 microns. Steve A. McClain, MD 631 361 4000 -----Original Message----- From: Blazek, Linda [mailto:lblazek at digestivespecialists.com] Sent: Wednesday, July 08, 2015 2:40 PM To: Steve McClain; histonet at lists.utsouthwestern.edu Subject: RE: Histonet Digest, Vol 140, Issue 6 Specimen loss Dear Mr. Cantankerous, :) So how would you gross a colon biopsy that was <0.1 cm and probably mucus? -----Original Message----- From: Steve McClain [mailto:SteveM at mcclainlab.com] Sent: Wednesday, July 08, 2015 1:47 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss My goodness 7% tissue loss is ridiculous. What fool published that? A 0.8% "loss of tissue" is beyond me. Would any one advertise in the local paper that your center of excellence lab loses nearly 1% of your specimens? "possibly too small to survive processing" is in my opinion, a self-fulfilling prophesy, one to be eschewed and denounced, or beaten out of the grosser who dares use that description for it is an expectation of failure. That grosser would not last a week around me. I would walk them to the train station and ask them not to return. I felt less cantankerous before I read that one. Steve A. McClain, MD 631 361 4000 **** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From travers.3 at osu.edu Thu Jul 9 07:45:48 2015 From: travers.3 at osu.edu (Travers, Susan) Date: Thu, 9 Jul 2015 12:45:48 +0000 Subject: [Histonet] subbing positively charged slides Message-ID: Does anyone know if it's ok to sub positively charged slides with chrome alum-gelatin. I seem to remember an incident where this did not work- but maybe it's just superstition... it seems as if the chrome alum might be positively charged itself and thus repelled from the surface of the slide... Susan Travers Professor, Biosciences Division of Biosciences, College of Dentistry The Ohio State University 305 West 12th Avenue Columbus, Ohio 43210 travers.3 at osu.edu (614)361-0800 (cell) (614)292-6366 (work) From relia1 at earthlink.net Thu Jul 9 08:13:17 2015 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 9 Jul 2015 09:13:17 -0400 Subject: [Histonet] RELIA HOT HISTOLOGY JOB ALERT HISTOLOGY SUPERVISOR - DAYS !!! - DALLAS TEXAS A RELIA EXCLUSIVE!!! Message-ID: <004f01d0ba49$055156a0$0ff403e0$@earthlink.net> Hi Histonetters!! I hope you are having a great day. I have an exciting opportunity to share- RELIA has been engaged on an EXCLUSIVE search for a Histology Supervisor for a leading lab in the Dallas area. ASCP certification and experience managing a group of 30+ people is required. This is a GREAT company to work for. They offer an EXCELLENT compensation and benefits package and a really nice group of people eager for a supervisor to join their team and lead them! If you or someone you know is interested in hearing more about this opportunity please contact me ASAP!! The best way to reach me is on my cell at 407-353-5070 or by email at relia1 at earthlink.net you can also call the office toll free at 866-607-3542. If you or someone you know is interested in this opportunity I want to hear from you and so does my client!! Have a great day and thanks for taking the time to read my post! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From a.boanas at epistem.co.uk Thu Jul 9 08:15:33 2015 From: a.boanas at epistem.co.uk (Adam Boanas) Date: Thu, 9 Jul 2015 13:15:33 +0000 Subject: [Histonet] Coverslipping mystery Message-ID: Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance when this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX From rjbuesa at yahoo.com Thu Jul 9 08:47:14 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 9 Jul 2015 13:47:14 +0000 (UTC) Subject: [Histonet] Coverslipping mystery In-Reply-To: References: Message-ID: <1704519404.1832644.1436449634381.JavaMail.yahoo@mail.yahoo.com> Adam: Just guessing, but I think the "mystery" is caused by how fluid the DPX is. Guessing again, but it probably is?more dense as it should.I would dilute it to the lowest density it can be used in your coverslipper. Give it a try.Ren? On Thursday, July 9, 2015 9:40 AM, Adam Boanas wrote: Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance when this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one!? - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From simmca at UPMC.EDU Thu Jul 9 08:49:00 2015 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Thu, 9 Jul 2015 13:49:00 +0000 Subject: [Histonet] Coverslipping mystery In-Reply-To: <1704519404.1832644.1436449634381.JavaMail.yahoo@mail.yahoo.com> References: <1704519404.1832644.1436449634381.JavaMail.yahoo@mail.yahoo.com> Message-ID: <6295CCF61B0DBB4C9248F51F8940E6A1011FD44E@MSXMBXNSPRD09.acct.upmchs.net> Look in your manual, near the back. There should be mountant type calibration settings for yo to adjust the machine to. That, hopefully will solve your issue! Chris Simmons B.S., A.S., HTL(ASCP) Supervisor, UPP Dermatopathology 412.864.3880 office 412.612.0881 cell -----Original Message----- From: Rene J Buesa [mailto:rjbuesa at yahoo.com] Sent: Thursday, July 09, 2015 9:47 AM To: Adam Boanas; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Coverslipping mystery Adam: Just guessing, but I think the "mystery" is caused by how fluid the DPX is. Guessing again, but it probably is?more dense as it should.I would dilute it to the lowest density it can be used in your coverslipper. Give it a try.Ren? On Thursday, July 9, 2015 9:40 AM, Adam Boanas wrote: Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance when this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one!? - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins at mt.gov Thu Jul 9 08:52:08 2015 From: TGoins at mt.gov (Goins, Tresa) Date: Thu, 9 Jul 2015 13:52:08 +0000 Subject: [Histonet] Coverslipping mystery In-Reply-To: References: Message-ID: I am not familiar with DPX, but is there a compatibility issue between it and the slide clearant you are using? Tresa -----Original Message----- From: Adam Boanas [mailto:a.boanas at epistem.co.uk] Sent: Thursday, July 09, 2015 7:16 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Coverslipping mystery Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance when this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff at rwjms.rutgers.edu Thu Jul 9 09:07:00 2015 From: mcauliff at rwjms.rutgers.edu (Geoff) Date: Thu, 09 Jul 2015 10:07:00 -0400 Subject: [Histonet] subbing positively charged slides In-Reply-To: References: Message-ID: <559E8004.1000607@umdnj.edu> Why would you want to do this? You could buy plain slides and sub them yourself. Geoff On 7/9/2015 8:45 AM, Travers, Susan wrote: > Does anyone know if it's ok to sub positively charged slides with chrome alum-gelatin. I seem to remember an incident where this did not work- but maybe it's just superstition... it seems as if the chrome alum might be positively charged itself and thus repelled from the surface of the slide... > > Susan Travers > Professor, Biosciences > Division of Biosciences, College of Dentistry > The Ohio State University > 305 West 12th Avenue > Columbus, Ohio 43210 > travers.3 at osu.edu > (614)361-0800 (cell) > (614)292-6366 (work) > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff at rwjms.rutgers.edu ********************************************** From jkiernan at uwo.ca Thu Jul 9 09:12:49 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Thu, 09 Jul 2015 09:12:49 -0500 Subject: [Histonet] subbing positively charged slides In-Reply-To: <72e0ef414b6f.559e8125@uwo.ca> References: <73a0d57abd7.559e806b@uwo.ca> <72c0847231c1.559e80a7@uwo.ca> <72c097db4fb4.559e80e6@uwo.ca> <72e0ef414b6f.559e8125@uwo.ca> Message-ID: <7280a6b47c50.559e3b11@uwo.ca> Chrome-gelatin cannot be expected to stick well to a glass surface that has been modified by adding aminopropyl (or similar) groups to its silicate ions. See http://publish.uwo.ca/~jkiernan/adhesivs.htm. John Kiernan London, Canada = = = On 09/07/15, "Travers, Susan" wrote: > Does anyone know if it's ok to sub positively charged slides with chrome alum-gelatin. I seem to remember an incident where this did not work- but maybe it's just superstition... it seems as if the chrome alum might be positively charged itself and thus repelled from the surface of the slide... > > Susan Travers > Professor, Biosciences > Division of Biosciences, College of Dentistry > The Ohio State University > 305 West 12th Avenue > Columbus, Ohio 43210 > travers.3 at osu.edu> > (614)361-0800 (cell) > (614)292-6366 (work) > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From melissa at alliedsearchpartners.com Thu Jul 9 09:42:32 2015 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Thu, 9 Jul 2015 14:42:32 +0000 Subject: [Histonet] Direct Hire Histotech Needed in Santa Maria, CA Message-ID: Hello, I have a full time opening for a permanent/long term Histotech in the Santa Maria, CA area. Please contact me if you would like more details. Thank you! Check out our article in I4Biz June's Addition "Allied Search Partners connecting Labs to Employees" http://www.i4biz.com/sales-marketing/allied-search-partners-connects-labs-to-employees/ To view a complete list of Allied Search Partners current openings go to: http://www.jobs.net/jobs/alliedsearchpartners/en-us/all-jobs/United-States/ Melissa Owens (Phelan) President, Laboratory Staffing Allied Search Partners www.linkedin.com/in/melissaphelan/ http://www.alliedsearchpartners.com T: 888.388.7571 ext. 102 F: 888.388.7572 *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From PAMarcum at uams.edu Thu Jul 9 10:15:47 2015 From: PAMarcum at uams.edu (Marcum, Pamela A) Date: Thu, 9 Jul 2015 15:15:47 +0000 Subject: [Histonet] 2 Open Positions in Little Rock Arkansas Message-ID: WE currently have two open positions for either registered HT or HTL. Registry must be current and some experience. We are a heavily computerized Histology Laboratory and will require the persons applying to be comfortable with computers. We are a routine Histology Laboratory at the University of Arkansas for Medical Sciences, in Little Rock. This is a beautiful area in Central Arkansas with lots of things to do both indoors and especially outdoors. If you are interested please contact me with your resume as soon as possible. Please no recruiters, we are not allowed to use you, I am sorry. Thank You, Pam Marcum ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From histology81176 at att.net Thu Jul 9 11:20:09 2015 From: histology81176 at att.net (Histology Technician) Date: Thu, 9 Jul 2015 16:20:09 +0000 (UTC) Subject: [Histonet] Gross Room QC sheet Message-ID: <1290933179.1997863.1436458809680.JavaMail.yahoo@mail.yahoo.com> Does anyone have a Gross Room QC sheet you'd like to share?? Thanks so much! From histology81176 at att.net Thu Jul 9 11:22:47 2015 From: histology81176 at att.net (Histology Technician) Date: Thu, 9 Jul 2015 16:22:47 +0000 (UTC) Subject: [Histonet] Medica ELGA DV 25 Message-ID: <377965148.2007366.1436458967925.JavaMail.yahoo@mail.yahoo.com> Does anyone use the DI water system called Medica by Eqova?? We just recently got 2 in our lab and was wondering if anyone had an SOP you'd like to share?? Can you tell my what daily QC needs to be done with it?? Water tested?? Thanks! From anna.coffey at nih.gov Thu Jul 9 11:57:14 2015 From: anna.coffey at nih.gov (Coffey, Anna (NIH/NCI) [C]) Date: Thu, 9 Jul 2015 16:57:14 +0000 Subject: [Histonet] Roche B-gal Staining Kit Message-ID: <5C3E10119A1B824FBE92B08279F74A910259809B@msgb10.nih.gov> Hello, I have been asked to do a B-gal enzyme stain using a Roche kit on frozen mouse sections. Another histologist recommended post-fixing in a glutaraldehyde-formaldehyde solution although this isn't mentioned in the kit instructions. This has me worried that there may be other things missing from the kit instructions that could improve the results. Has anyone else had any experience using this kit and, if so, did you find that other alterations to the protocol were required? Also wondering about potential counterstains that might work with this one. I'd appreciate any pointers if you have them! Thanks! Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey at nih.gov 301-846-1730 From JMacDonald at mtsac.edu Thu Jul 9 11:53:06 2015 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Thu, 9 Jul 2015 09:53:06 -0700 Subject: [Histonet] Coverslipping mystery In-Reply-To: References: Message-ID: What type of clearing agent are you using? The aliphatic hydrocarbons are not compatible with all mounting media. From: Adam Boanas To: "histonet at lists.utsouthwestern.edu" Date: 07/09/2015 06:16 AM Subject: [Histonet] Coverslipping mystery Hello, We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance whe n this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? We are struggling for ideas with this one! - this occurs with fresh DPX also. Many thanks Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Thu Jul 9 12:45:24 2015 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 9 Jul 2015 13:45:24 -0400 Subject: [Histonet] Specimen loss Message-ID: Steve McClain writes: "In UPMC protocol They wrap all specimens, ink the tissue with eosin (erythrosine) at the grossing bench and cut two levels at 40 microns." Do NOT use eosin for this purpose. It's highly fluorescent, and will interfere with any method (such as FISH) that relies on fluorescence. Safranin O works well for this purpose - placing the specimens on those blue pads after marking them. It's easily available - this is the red component of the Gram stain done by any microbiology service. Bob Richmond Samurai Pathologist Maryville TN From mw at personifysearch.com Thu Jul 9 12:50:03 2015 From: mw at personifysearch.com (Matt Ward) Date: Thu, 9 Jul 2015 13:50:03 -0400 Subject: [Histonet] Field Histology Opportunity Message-ID: Hello Histonet! We have recently had a new position open with a world leading histology manufacturer. Our client is looking for a field based Histology Support Specialist to cover a region and provide support to their clients. The ideal candidate will have a background in histology and troubleshooting. This is a great opportunity for someone to break into the field! Region: VA, MD, NC, SC, TN, AR (based near an airport) Offers a base salary + bonus; company car, paid expenses, full benefits, PTO. Must be willing to travel. Please e-mail me directly at mw at personifysearch.com to learn more! Thanks! Matt Matt Ward *Program Manager ? RPO* *Personify * 5020 Weston Parkway Suite 315 Cary NC 27513 Direct Line: (919) 459-3654 Toll Free: (800) 875-6188 ext. 103 www.personifysearch.com From nlinke at sbch.org Thu Jul 9 13:53:45 2015 From: nlinke at sbch.org (Noelle Linke) Date: Thu, 9 Jul 2015 18:53:45 +0000 Subject: [Histonet] histotechs needed Message-ID: Hi all! I just had a couple of retirements (20+ years for both) and I am looking for 2 histotechs to come live and work in beautiful Santa Barbara, CA. If you are interested or know anyone who might be, please give me a shout! Thank you, No?lle No?lle Linke, MS, HTL(ASCP) QIHC Manager, Anatomic Pathology Pacific Diagnostic Laboratories nlinke at sbch.org Phone: (805) 324-9814 Fax: (805) 696-6433 ________________________________ CHS Disclaimer: This electronic mail message is intended exclusively for the individual or entity to which it is addressed. This message, together with any attachment, may contain confidential and privileged information. Any views, opinions or conclusions expressed in this message are those of the individual sender and do not necessarily reflect the views of Cottage Health System, its subsidiaries or affiliates. This document may also contain information covered under the Health Insurance Portability and Accountability Act (HIPAA, PL 104-191) and implementing regulations and must be protected in accordance with those provisions. Re-disclosure without patient consent or as otherwise permitted by law is prohibited. Any unauthorized review, retransmission, use, printing, copying, retention, disclosure, distribution or the taking of any action in reliance upon this information by persons or entities other than the intended recipient is strictly prohibited and may be unlawful. If you have received this message in error, please immediately advise the sender by reply email message to the sender and delete all copies of this message from your system without copying. ________________________________ From TanyaAbbott at catholichealth.net Thu Jul 9 14:09:42 2015 From: TanyaAbbott at catholichealth.net (Abbott, Tanya) Date: Thu, 9 Jul 2015 19:09:42 +0000 Subject: [Histonet] AFB contaminant Message-ID: <852F7D2C14FB464D80E182B15DB138AF6B7E72E9@CHIEX005.CHI.catholichealth.net> Has anyone ever had a problem with a possible contaminant in their AFB hand stain? If so, how did you deal with it? Any suggestions? Ours actually looked like an AFB like organism, which we later determined to be negative. Thanks! Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From galinadeyneko at yahoo.com Thu Jul 9 14:22:10 2015 From: galinadeyneko at yahoo.com (Galina Deyneko) Date: Thu, 9 Jul 2015 19:22:10 +0000 (UTC) Subject: [Histonet] XGal staining Message-ID: <1712864210.2427525.1436469730169.JavaMail.yahoo@mail.yahoo.com> ?Dear All, and Anna.I am confused about the recommendation of post fixation (stained slides?)?in glutaraldehyde-foormaldehyde solution. According many protocols and my experience the fresh tissues? should be fixed in in glutaraldehyde -formalin solution that is mproved the tissue morphology and staining quality.The best counterstaining is Nuclear Fast Red from Vector. ??Galina DeynekoNovartis, Cambridge, MA?617-871-7613 w From kkienitz at orclinic.com Thu Jul 9 14:47:27 2015 From: kkienitz at orclinic.com (Kienitz, Kari) Date: Thu, 9 Jul 2015 12:47:27 -0700 Subject: [Histonet] AFB contaminant In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF6B7E72E9@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF6B7E72E9@CHIEX005.CHI.catholichealth.net> Message-ID: <41400FFE517878449D89114DD2526090196602D1B9@tocmail1.tocad.orclinic.com> most likely a fungus ball, confirm with a PAS Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz at orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: Abbott, Tanya [TanyaAbbott at catholichealth.net] Sent: Thursday, July 09, 2015 12:09 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] AFB contaminant Has anyone ever had a problem with a possible contaminant in their AFB hand stain? If so, how did you deal with it? Any suggestions? Ours actually looked like an AFB like organism, which we later determined to be negative. Thanks! Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew at shaw.ca Thu Jul 9 15:03:41 2015 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Thu, 09 Jul 2015 13:03:41 -0700 Subject: [Histonet] AFB contaminant In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF6B7E72E9@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF6B7E72E9@CHIEX005.CHI.catholichealth.net> Message-ID: <559ED39D.2090302@shaw.ca> Hi, This may be AFB contamination from tap water. The organisms grow in the end of cold water taps. You should take the taps apart and thoroughly descale and scrub out. The organisms may be somewhat longer and thicker than TB, and may be quite distinctly beaded. Bryan Llewellyn Abbott, Tanya wrote: > Has anyone ever had a problem with a possible contaminant in their AFB hand stain? If so, how did you deal with it? Any suggestions? > Ours actually looked like an AFB like organism, which we later determined to be negative. > Thanks! > > Tanya G. Abbott > Manager Technologist > Histology/Cytology > St Joseph Medical Center > (phone) 610-378-2635 > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Timothy.Morken at ucsf.edu Thu Jul 9 15:36:05 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 9 Jul 2015 20:36:05 +0000 Subject: [Histonet] AFB contaminant In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF6B7E72E9@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF6B7E72E9@CHIEX005.CHI.catholichealth.net> Message-ID: <761E2B5697F795489C8710BCC72141FF602F7219@ex07.net.ucsf.edu> We had some a few years ago and thought it was contaminating bacteria, but turned out to be dirty water from the di tap. We had our plumbers take it apart and clean everything. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Abbott, Tanya [mailto:TanyaAbbott at catholichealth.net] Sent: Thursday, July 09, 2015 12:10 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] AFB contaminant Has anyone ever had a problem with a possible contaminant in their AFB hand stain? If so, how did you deal with it? Any suggestions? Ours actually looked like an AFB like organism, which we later determined to be negative. Thanks! Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology81176 at att.net Thu Jul 9 15:51:12 2015 From: histology81176 at att.net (Histology Technician) Date: Thu, 9 Jul 2015 20:51:12 +0000 (UTC) Subject: [Histonet] Ventana antibody - H pylori Message-ID: <1324954415.2157331.1436475072267.JavaMail.yahoo@mail.yahoo.com> Does anyone else have constant problems with the Ventana antibody H pylori having a dirty background stain?? My pathologists are constantly complaining about it and when I call CS I get the same response...decon the instrument, try a new lot number, etc... it's never truly resolved.? Any thoughts?? Thanks! From Richard.Cartun at hhchealth.org Thu Jul 9 16:05:51 2015 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Thu, 9 Jul 2015 21:05:51 +0000 Subject: [Histonet] Ventana antibody - H pylori In-Reply-To: <1324954415.2157331.1436475072267.JavaMail.yahoo@mail.yahoo.com> References: <1324954415.2157331.1436475072267.JavaMail.yahoo@mail.yahoo.com> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E5E7C2054@HHCEXCHMB03.hhcsystem.org> Is the Ventana antibody polyclonal or monoclonal? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Histology Technician [mailto:histology81176 at att.net] Sent: Thursday, July 09, 2015 4:51 PM To: Histonet; histonet-request at lists.utsouthwestern.edu Subject: [Histonet] Ventana antibody - H pylori Does anyone else have constant problems with the Ventana antibody H pylori having a dirty background stain? My pathologists are constantly complaining about it and when I call CS I get the same response...decon the instrument, try a new lot number, etc... it's never truly resolved. Any thoughts? Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From LSebree at uwhealth.org Thu Jul 9 16:16:09 2015 From: LSebree at uwhealth.org (Sebree Linda A) Date: Thu, 9 Jul 2015 21:16:09 +0000 Subject: [Histonet] Ventana antibody - H pylori In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E5E7C2054@HHCEXCHMB03.hhcsystem.org> References: <1324954415.2157331.1436475072267.JavaMail.yahoo@mail.yahoo.com> <9215BD4B0BA1B44D962A71C758B68D2E5E7C2054@HHCEXCHMB03.hhcsystem.org> Message-ID: <77DD817201982748BC67D7960F2F76AF14C3C0@UWHC-MBX12.uwhis.hosp.wisc.edu> Rabbit monoclonal, Richard. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] Sent: Thursday, July 09, 2015 4:06 PM To: Histology Technician; Histonet; histonet-request at lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana antibody - H pylori Is the Ventana antibody polyclonal or monoclonal? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Histology Technician [mailto:histology81176 at att.net] Sent: Thursday, July 09, 2015 4:51 PM To: Histonet; histonet-request at lists.utsouthwestern.edu Subject: [Histonet] Ventana antibody - H pylori Does anyone else have constant problems with the Ventana antibody H pylori having a dirty background stain? My pathologists are constantly complaining about it and when I call CS I get the same response...decon the instrument, try a new lot number, etc... it's never truly resolved. Any thoughts? Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LSebree at uwhealth.org Thu Jul 9 16:17:28 2015 From: LSebree at uwhealth.org (Sebree Linda A) Date: Thu, 9 Jul 2015 21:17:28 +0000 Subject: [Histonet] Ventana antibody - H pylori In-Reply-To: <1324954415.2157331.1436475072267.JavaMail.yahoo@mail.yahoo.com> References: <1324954415.2157331.1436475072267.JavaMail.yahoo@mail.yahoo.com> Message-ID: <77DD817201982748BC67D7960F2F76AF14C3CB@UWHC-MBX12.uwhis.hosp.wisc.edu> We have excellent and very clean results with the VMS H. Pylori on our Ultras. I can shoot you our protocol if you're interested. Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Histology Technician [mailto:histology81176 at att.net] Sent: Thursday, July 09, 2015 3:51 PM To: Histonet; histonet-request at lists.utsouthwestern.edu Subject: [Histonet] Ventana antibody - H pylori Does anyone else have constant problems with the Ventana antibody H pylori having a dirty background stain?? My pathologists are constantly complaining about it and when I call CS I get the same response...decon the instrument, try a new lot number, etc... it's never truly resolved.? Any thoughts?? Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mills at 3scan.com Thu Jul 9 16:28:29 2015 From: mills at 3scan.com (Caroline Miller) Date: Thu, 9 Jul 2015 14:28:29 -0700 Subject: [Histonet] Histology phantoms? Message-ID: Hi Histonet! Happy 'almost' Friday :) Can I ask if anyone out there has ever put phantoms or other fiduciary markings into their paraffin blocks to help with registering serial sectioned images or other downstream processes? thanks in advance! yours, mills -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From Behnaz.Sohrab at ah.org Thu Jul 9 17:06:48 2015 From: Behnaz.Sohrab at ah.org (Sohrab,Behnaz) Date: Thu, 9 Jul 2015 22:06:48 +0000 Subject: [Histonet] HISTO POSTION Message-ID: <92096F092E8CE54FBB5E272198FE5A7E029D2F29@ahexcms001.ah.org> We are hiring full time Histologist with mini 5 year experience in all aspects of histology ,especially IHC and Breast Panels. Behnaz Sohrab Regional Manager Anatomic pathology Behnaz.Sohrab at ah.org 323-268-5000 Ext.1711 323-265-5086 FAX From sccrshlly at yahoo.com Thu Jul 9 21:12:57 2015 From: sccrshlly at yahoo.com (Shelly Coker) Date: Fri, 10 Jul 2015 02:12:57 +0000 (UTC) Subject: [Histonet] Specimens lost in process Message-ID: <59421897.2680484.1436494377374.JavaMail.yahoo@mail.yahoo.com> Steven, Here are some thought I have on each of these from the perspective of managing a large GI lab: 1.? I have only had one specimen that was lost by the grossers, and it wasn't lost.? The cassette went into the bin with the specimen containers for the day.? This is where I found the non-viable specimen the following morning, all dried up.? We don't lose them to the biotrash because we don't use paper towels at all.? We use Lab Wipes which are thicker and it is harder for items to get accidentally thrown away.? Another trick we do is to wrap ALL specimens in perm papers.? The papers are more porous than regular filter paper, and we have a Fuji paper dispenser that works like a tissue box does.? The gross techs love them.? And they fold the paper so that the techs only have to pull on two corners to open when embedding. 2.? The perm papers completely alleviated not only lost specimens, but also all cross contamination from processing, which we saw frequently with esophageal tissue. 3.? You do not want to use micromesh....We did without knowing it was not advisable.? All of our small bowel specimens showed? what our pathologist called "a blown up effect" because the water stays trapped in the cassette because the holes are so small.? Then the water super heats and boils out, tearing the epithelium from the other layers, making a clear diagnosis difficult. 4.? We do have this problem, but have found that upon checking the gross, the specimen was so small it was not likely to survive processing.? Now, that does not include if the tech accidentally opens the cassette too quickly and the tissue flips off somewhere.? This can happen with perm paper, but it is much less likely. 5.? The microtomy problem is what it is .? Encourage staff to seek help before it comes to that. We have less than .05% of specimens per month that we call "did not survive processing".? I always communicate directly with the surgery center manager if the specimen started of inadequate, but if the error is ours I call the physician directly with the explanation.? We also do an incident report with requires investigation on my part as well as a root cause analysis. Hope this helps...if you are interested in the perm paper solution, head down to Sally's Beauty supply and look for Fuji Perfect Paper End Wrappers and the Hands Free Dispenser.? You can also find them on Amazon. Regards,Shelly CokerLab ManagerAustin Gastroenterology Looking for help in analyzing the entire scope of the process. There is not much published data (that I can find) and I am hoping this group can lend some expertise. Our rate is higher than we would like it to be. There is no consistent size at risk although GI and Derm biopsies are the biggest involved group. We have broken it down into steps. 1.? ? ? Can be lost at grossing- either never loaded into the cassette at all, or cassette was discarded. Thus we hold on to our waste and can search for misdirected cassettes if need be. 2.? ? ? Lost in the processor itself. Most are wrapped. If large enough not to be wrapped, we would not expect the processor to eat them, so assume cassette lid not properly closed. Frankly the highest number of losses we're seeing is no tissue found in cassette by embedders. 3.? ? ? I am being told that we can't use micromesh cassettes in our microwave processors (Milestone Pathos) and want to know if anyone is. 4.? ? ? Tissue not seen at embedding. Again no way to tell when the tissue disappeared. We know that tiny tissue can spring out during the opening at embedding but I don't know how else to examine or limit this step. 5.? ? ? Tissue can be exhausted during microtomy. Rare but noteworthy. I am hoping people can tell me about their procedures for dealing with "specimens that don't survive processing", what safeguards they have in place, and to some extent what your own lab percentage? or experience is. Apologies in advance for the length of the message, but could really use your help. Steven Pinheiro, MBA, MLS(ASCP)DLMCM Manager Anatomic Pathology and Cytology Loyola University Medical Center 2160 S First Ave, Bldg 110 Rm 2214 Maywood, IL 60153 708-327-2642 (O) 708-327-2620 (F) spinheiro at lumc.edu From sforeman at labpath.com Fri Jul 10 07:07:59 2015 From: sforeman at labpath.com (Susan Foreman) Date: Fri, 10 Jul 2015 08:07:59 -0400 Subject: [Histonet] Ventana antibody - H pylori In-Reply-To: <77DD817201982748BC67D7960F2F76AF14C3C0@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <1324954415.2157331.1436475072267.JavaMail.yahoo@mail.yahoo.com> <9215BD4B0BA1B44D962A71C758B68D2E5E7C2054@HHCEXCHMB03.hhcsystem.org> <77DD817201982748BC67D7960F2F76AF14C3C0@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: <004801d0bb09$10e30e80$32a92b80$@labpath.com> We have great luck with Cell Marque antibody on our Ventana Benchmark Ultra using UltraView DAB detection. He have had dirty staining for spirochetes (syphilis) in the past utilizing the Red Detection kit and decons do seem to help a bit. The DAB detection kit is much cleaner. -----Original Message----- From: Sebree Linda A [mailto:LSebree at uwhealth.org] Sent: Thursday, July 09, 2015 5:16 PM To: 'Cartun, Richard'; Histology Technician; Histonet; histonet-request at lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana antibody - H pylori Rabbit monoclonal, Richard. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] Sent: Thursday, July 09, 2015 4:06 PM To: Histology Technician; Histonet; histonet-request at lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana antibody - H pylori Is the Ventana antibody polyclonal or monoclonal? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Histology Technician [mailto:histology81176 at att.net] Sent: Thursday, July 09, 2015 4:51 PM To: Histonet; histonet-request at lists.utsouthwestern.edu Subject: [Histonet] Ventana antibody - H pylori Does anyone else have constant problems with the Ventana antibody H pylori having a dirty background stain? My pathologists are constantly complaining about it and when I call CS I get the same response...decon the instrument, try a new lot number, etc... it's never truly resolved. Any thoughts? Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Fri Jul 10 14:25:56 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 10 Jul 2015 19:25:56 +0000 Subject: [Histonet] Sakura SmartSection Message-ID: <761E2B5697F795489C8710BCC72141FF602F7596@ex07.net.ucsf.edu> Has anyone done any work with the Sakura SmartSection robot? We've had some blocks cut on it and have had good initial results. This could be a game-changer for histology staffing. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From simmca at UPMC.EDU Fri Jul 10 14:43:37 2015 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Fri, 10 Jul 2015 19:43:37 +0000 Subject: [Histonet] Sakura SmartSection In-Reply-To: <761E2B5697F795489C8710BCC72141FF602F7596@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF602F7596@ex07.net.ucsf.edu> Message-ID: <48BF2378-7104-46CA-9C0A-E0C442FB01E4@UPMC.EDU> Have it smart section undecalcified bone or teeth in plastic then I might give it a look But only a look You can never take the art away from the technicians Ever Sent from my iPhone > On Jul 10, 2015, at 3:28 PM, Morken, Timothy wrote: > > Has anyone done any work with the Sakura SmartSection robot? We've had some blocks cut on it and have had good initial results. This could be a game-changer for histology staffing. > > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Fri Jul 10 14:58:17 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 10 Jul 2015 19:58:17 +0000 Subject: [Histonet] Sakura SmartSection In-Reply-To: <48BF2378-7104-46CA-9C0A-E0C442FB01E4@UPMC.EDU> References: <761E2B5697F795489C8710BCC72141FF602F7596@ex07.net.ucsf.edu> <48BF2378-7104-46CA-9C0A-E0C442FB01E4@UPMC.EDU> Message-ID: <761E2B5697F795489C8710BCC72141FF602F75DE@ex07.net.ucsf.edu> Definitely it's not going to be the best solution for everything in histology. Routine cutting will be the main task, and controls slides are a natural. Considering the trouble we have finding good histotechs, a robot that works continuously can really take the pressure off the routine work and let our techs do the more complicated work. Consider this as well: Sakura is redesigning its entire instrument line to allow robotic handoffs between them. We will see a totally robotic histology lab (tissue processing to H&E) in the near future. Tim -----Original Message----- From: Simmons, Christopher [mailto:simmca at UPMC.EDU] Sent: Friday, July 10, 2015 12:44 PM To: Morken, Timothy Cc: Histonet Subject: Re: [Histonet] Sakura SmartSection Have it smart section undecalcified bone or teeth in plastic then I might give it a look But only a look You can never take the art away from the technicians Ever Sent from my iPhone > On Jul 10, 2015, at 3:28 PM, Morken, Timothy wrote: > > Has anyone done any work with the Sakura SmartSection robot? We've had some blocks cut on it and have had good initial results. This could be a game-changer for histology staffing. > > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology UC San Francisco Medical Center > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper at chla.usc.edu Fri Jul 10 14:59:43 2015 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Fri, 10 Jul 2015 19:59:43 +0000 Subject: [Histonet] Sakura SmartSection In-Reply-To: <761E2B5697F795489C8710BCC72141FF602F7596@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF602F7596@ex07.net.ucsf.edu> Message-ID: I went to Sakura to see that thing on a demo a few years back, and it was . . . scary! The sales reps couldn't get it calibrated right, and it ripped tissue out of the blocks while it was trimming on a few occasions. Not one of us in the room (pretty much all experienced bench histotechs) could collect ribbons, though I suspect this could have been a learning curve type thing. Aside from that, I will say that they had the ability to realign blocks pretty well perfected, and I was impressed by that. I went back to Sakura about a year later for something else, and our rep was still shaking her head about how bad that demo experience went! Sounds like they may have worked out the kinks eh? Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357??? bcooper at chla.usc.edu -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Friday, July 10, 2015 12:26 PM To: Histonet Subject: [Histonet] Sakura SmartSection Has anyone done any work with the Sakura SmartSection robot? We've had some blocks cut on it and have had good initial results. This could be a game-changer for histology staffing. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From Terry.Dyer at tmmc.com Fri Jul 10 15:15:30 2015 From: Terry.Dyer at tmmc.com (Dyer, Terry) Date: Fri, 10 Jul 2015 20:15:30 +0000 Subject: [Histonet] Ventana antibody - H pylori In-Reply-To: <004801d0bb09$10e30e80$32a92b80$@labpath.com> References: <1324954415.2157331.1436475072267.JavaMail.yahoo@mail.yahoo.com> <9215BD4B0BA1B44D962A71C758B68D2E5E7C2054@HHCEXCHMB03.hhcsystem.org> <77DD817201982748BC67D7960F2F76AF14C3C0@UWHC-MBX12.uwhis.hosp.wisc.edu> <004801d0bb09$10e30e80$32a92b80$@labpath.com> Message-ID: <52B7E4673B4A6045988BC7065CA3B84815AD8D2F@EXCH-2.tmmc.com> Happy Friday1 We are experiencing the same problem. Just had a site visit from Ventana and they suggested using an antibody dilution buffer. Use an option kit filled with Ventana Product ADB250 (antibody dilution buffer). Check options in protocol editing and 8 minutes, suggested time. Have not tried it but inted to. Good luck. -----Original Message----- From: Susan Foreman [mailto:sforeman at labpath.com] Sent: Friday, July 10, 2015 5:08 AM To: 'Sebree Linda A'; 'Cartun, Richard'; 'Histology Technician'; 'Histonet'; histonet-request at lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana antibody - H pylori We have great luck with Cell Marque antibody on our Ventana Benchmark Ultra using UltraView DAB detection. He have had dirty staining for spirochetes (syphilis) in the past utilizing the Red Detection kit and decons do seem to help a bit. The DAB detection kit is much cleaner. -----Original Message----- From: Sebree Linda A [mailto:LSebree at uwhealth.org] Sent: Thursday, July 09, 2015 5:16 PM To: 'Cartun, Richard'; Histology Technician; Histonet; histonet-request at lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana antibody - H pylori Rabbit monoclonal, Richard. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] Sent: Thursday, July 09, 2015 4:06 PM To: Histology Technician; Histonet; histonet-request at lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana antibody - H pylori Is the Ventana antibody polyclonal or monoclonal? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Histology Technician [mailto:histology81176 at att.net] Sent: Thursday, July 09, 2015 4:51 PM To: Histonet; histonet-request at lists.utsouthwestern.edu Subject: [Histonet] Ventana antibody - H pylori Does anyone else have constant problems with the Ventana antibody H pylori having a dirty background stain? My pathologists are constantly complaining about it and when I call CS I get the same response...decon the instrument, try a new lot number, etc... it's never truly resolved. Any thoughts? Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=c09Z9T_vWDxmFqobefbEMtlmpncr97OXXiuIfi6sSaw&r=Fh4KlUv_UqKN03sLo_qOgprEKoaSMs5dhn6xDjQ2Oaw&m=RfpXFJ48d3Jhw7L-dEkoNFuBrh2v-_U9uU-J1XmgaI4&s=dItbdub7yicLmpNzAOMuUtJIu1qXu7vhqfR1DsNyAm4&e= This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=c09Z9T_vWDxmFqobefbEMtlmpncr97OXXiuIfi6sSaw&r=Fh4KlUv_UqKN03sLo_qOgprEKoaSMs5dhn6xDjQ2Oaw&m=RfpXFJ48d3Jhw7L-dEkoNFuBrh2v-_U9uU-J1XmgaI4&s=dItbdub7yicLmpNzAOMuUtJIu1qXu7vhqfR1DsNyAm4&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=c09Z9T_vWDxmFqobefbEMtlmpncr97OXXiuIfi6sSaw&r=Fh4KlUv_UqKN03sLo_qOgprEKoaSMs5dhn6xDjQ2Oaw&m=RfpXFJ48d3Jhw7L-dEkoNFuBrh2v-_U9uU-J1XmgaI4&s=dItbdub7yicLmpNzAOMuUtJIu1qXu7vhqfR1DsNyAm4&e= From Donna.Willis at baylorhealth.edu Fri Jul 10 15:46:47 2015 From: Donna.Willis at baylorhealth.edu (Willis, Donna G.) Date: Fri, 10 Jul 2015 20:46:47 +0000 Subject: [Histonet] Sakura SmartSection In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF602F7596@ex07.net.ucsf.edu> Message-ID: <2572B4D63B62E64A8078D8BBE34D40788DB759C7@BHDASVEXML2.bhcs.pvt> Brian, I think the unit that Tim is talking about is something different. His unit has not need for a tech to pick up sections. The sections are placed on the slide by the robot. We are currently demoing the new Sakura Tissue Tek Auto Section Microtome that does not use a robot. I will have to say that if it is the same unit that you looked at years ago, they have worked out the bugs. It is a really nice unit. I technicians do not want the unit to leave when the demo is complete. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com -----Original Message----- From: Cooper, Brian [mailto:bcooper at chla.usc.edu] Sent: Friday, July 10, 2015 3:00 PM To: Morken, Timothy; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Sakura SmartSection I went to Sakura to see that thing on a demo a few years back, and it was . . . scary! The sales reps couldn't get it calibrated right, and it ripped tissue out of the blocks while it was trimming on a few occasions. Not one of us in the room (pretty much all experienced bench histotechs) could collect ribbons, though I suspect this could have been a learning curve type thing. Aside from that, I will say that they had the ability to realign blocks pretty well perfected, and I was impressed by that. I went back to Sakura about a year later for something else, and our rep was still shaking her head about how bad that demo experience went! Sounds like they may have worked out the kinks eh? Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Friday, July 10, 2015 12:26 PM To: Histonet Subject: [Histonet] Sakura SmartSection Has anyone done any work with the Sakura SmartSection robot? We've had some blocks cut on it and have had good initial results. This could be a game-changer for histology staffing. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=bKZPIq7CMd5nofh_6PpWlhPjBnWHJcPszndK-_NoX0s&s=nMwE-aXCRu2omFYsrK30C0y2SkmCo8U3KR2uQuV5uos&e= --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=bKZPIq7CMd5nofh_6PpWlhPjBnWHJcPszndK-_NoX0s&s=nMwE-aXCRu2omFYsrK30C0y2SkmCo8U3KR2uQuV5uos&e= ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From histology81176 at att.net Fri Jul 10 15:53:53 2015 From: histology81176 at att.net (Histology Technician) Date: Fri, 10 Jul 2015 20:53:53 +0000 (UTC) Subject: [Histonet] Log sheet for cutting control slides Message-ID: <152169718.81032.1436561633243.JavaMail.yahoo@mail.yahoo.com> Does anyone have a protocol for cutting control slides?? We are a GI lab and use patient tissue as our Gastric control for HP's and the past couple of months we've had a bad control block pulled (turned out to be colon) and had to repeat a lot.? Thought about doing a log sheet for the tech to date, sign off on, list the block # cut, and get the path to sign off on a test slide before we use that block for a control.? Any thoughts? Thanks! From bcooper at chla.usc.edu Fri Jul 10 16:40:17 2015 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Fri, 10 Jul 2015 21:40:17 +0000 Subject: [Histonet] Sakura SmartSection In-Reply-To: <2572B4D63B62E64A8078D8BBE34D40788DB759C7@BHDASVEXML2.bhcs.pvt> References: <761E2B5697F795489C8710BCC72141FF602F7596@ex07.net.ucsf.edu> <2572B4D63B62E64A8078D8BBE34D40788DB759C7@BHDASVEXML2.bhcs.pvt> Message-ID: Ah! I stand corrected. I have to admit, I'm really leery about letting automated microtomes be in the same vicinity of renal or liver needle biopsies--are you guys comfortable with this? Brian -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis at baylorhealth.edu] Sent: Friday, July 10, 2015 1:47 PM To: Cooper, Brian; Morken, Timothy; histonet at lists.utsouthwestern.edu Subject: RE: Sakura SmartSection Brian, I think the unit that Tim is talking about is something different. His unit has not need for a tech to pick up sections. The sections are placed on the slide by the robot. We are currently demoing the new Sakura Tissue Tek Auto Section Microtome that does not use a robot. I will have to say that if it is the same unit that you looked at years ago, they have worked out the bugs. It is a really nice unit. I technicians do not want the unit to leave when the demo is complete. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com -----Original Message----- From: Cooper, Brian [mailto:bcooper at chla.usc.edu] Sent: Friday, July 10, 2015 3:00 PM To: Morken, Timothy; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Sakura SmartSection I went to Sakura to see that thing on a demo a few years back, and it was . . . scary! The sales reps couldn't get it calibrated right, and it ripped tissue out of the blocks while it was trimming on a few occasions. Not one of us in the room (pretty much all experienced bench histotechs) could collect ribbons, though I suspect this could have been a learning curve type thing. Aside from that, I will say that they had the ability to realign blocks pretty well perfected, and I was impressed by that. I went back to Sakura about a year later for something else, and our rep was still shaking her head about how bad that demo experience went! Sounds like they may have worked out the kinks eh? Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Friday, July 10, 2015 12:26 PM To: Histonet Subject: [Histonet] Sakura SmartSection Has anyone done any work with the Sakura SmartSection robot? We've had some blocks cut on it and have had good initial results. This could be a game-changer for histology staffing. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=bKZPIq7CMd5nofh_6PpWlhPjBnWHJcPszndK-_NoX0s&s=nMwE-aXCRu2omFYsrK30C0y2SkmCo8U3KR2uQuV5uos&e= --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=bKZPIq7CMd5nofh_6PpWlhPjBnWHJcPszndK-_NoX0s&s=nMwE-aXCRu2omFYsrK30C0y2SkmCo8U3KR2uQuV5uos&e= ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From Donna.Willis at baylorhealth.edu Fri Jul 10 16:46:14 2015 From: Donna.Willis at baylorhealth.edu (Willis, Donna G.) Date: Fri, 10 Jul 2015 21:46:14 +0000 Subject: [Histonet] Sakura SmartSection In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF602F7596@ex07.net.ucsf.edu> <2572B4D63B62E64A8078D8BBE34D40788DB759C7@BHDASVEXML2.bhcs.pvt> Message-ID: <2572B4D63B62E64A8078D8BBE34D40788DB75A92@BHDASVEXML2.bhcs.pvt> I have seen more issues from the human side than automation. Currently all instruments on the market have to still have a technician assist with the microtomy. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com -----Original Message----- From: Cooper, Brian [mailto:bcooper at chla.usc.edu] Sent: Friday, July 10, 2015 4:40 PM To: Willis, Donna G.; Morken, Timothy; histonet at lists.utsouthwestern.edu Subject: RE: Sakura SmartSection Ah! I stand corrected. I have to admit, I'm really leery about letting automated microtomes be in the same vicinity of renal or liver needle biopsies--are you guys comfortable with this? Brian -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis at baylorhealth.edu] Sent: Friday, July 10, 2015 1:47 PM To: Cooper, Brian; Morken, Timothy; histonet at lists.utsouthwestern.edu Subject: RE: Sakura SmartSection Brian, I think the unit that Tim is talking about is something different. His unit has not need for a tech to pick up sections. The sections are placed on the slide by the robot. We are currently demoing the new Sakura Tissue Tek Auto Section Microtome that does not use a robot. I will have to say that if it is the same unit that you looked at years ago, they have worked out the bugs. It is a really nice unit. I technicians do not want the unit to leave when the demo is complete. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com -----Original Message----- From: Cooper, Brian [mailto:bcooper at chla.usc.edu] Sent: Friday, July 10, 2015 3:00 PM To: Morken, Timothy; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Sakura SmartSection I went to Sakura to see that thing on a demo a few years back, and it was . . . scary! The sales reps couldn't get it calibrated right, and it ripped tissue out of the blocks while it was trimming on a few occasions. Not one of us in the room (pretty much all experienced bench histotechs) could collect ribbons, though I suspect this could have been a learning curve type thing. Aside from that, I will say that they had the ability to realign blocks pretty well perfected, and I was impressed by that. I went back to Sakura about a year later for something else, and our rep was still shaking her head about how bad that demo experience went! Sounds like they may have worked out the kinks eh? Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Friday, July 10, 2015 12:26 PM To: Histonet Subject: [Histonet] Sakura SmartSection Has anyone done any work with the Sakura SmartSection robot? We've had some blocks cut on it and have had good initial results. This could be a game-changer for histology staffing. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=bKZPIq7CMd5nofh_6PpWlhPjBnWHJcPszndK-_NoX0s&s=nMwE-aXCRu2omFYsrK30C0y2SkmCo8U3KR2uQuV5uos&e= --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=bKZPIq7CMd5nofh_6PpWlhPjBnWHJcPszndK-_NoX0s&s=nMwE-aXCRu2omFYsrK30C0y2SkmCo8U3KR2uQuV5uos&e= ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From Steven.Swartwood at cshs.org Fri Jul 10 16:52:47 2015 From: Steven.Swartwood at cshs.org (Swartwood, Steven J) Date: Fri, 10 Jul 2015 21:52:47 +0000 Subject: [Histonet] Decalcification of mouse jaw and legs Message-ID: <959202AC61AEF942968646EC66E2BE3EACEA5CF0@ESPWMSGMBX08.CSMC.EDU> Hello all, I hope everyone is having or had a fantastic week, I'm going to test a few different decal methods on mouse jaws and leg bones. I was wondering if anyone does this routinely out there who is willing to share a protocol. I've never done this on mouse jaw/leg bones before. I've only done this on human tissues. From what I've read it seems that for routine histology low % HCl is fine, but if I wanted to perform IHC then anywhere from 5-15% formic acid may be the best way to go. Nitric acid is probably too strong of a decal agent for these tiny specimens I would assume. Maybe an EDTA based decal agent may be best as well. I'm just really inexperienced with this and I'm very open to ideas and trying a few different methods. Steven Swartwood HT(ASCP) IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is strictly prohibited. Thank you for your cooperation. From Timothy.Morken at ucsf.edu Fri Jul 10 16:56:22 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 10 Jul 2015 21:56:22 +0000 Subject: [Histonet] Sakura SmartSection In-Reply-To: <2572B4D63B62E64A8078D8BBE34D40788DB759C7@BHDASVEXML2.bhcs.pvt> References: <761E2B5697F795489C8710BCC72141FF602F7596@ex07.net.ucsf.edu> <2572B4D63B62E64A8078D8BBE34D40788DB759C7@BHDASVEXML2.bhcs.pvt> Message-ID: <761E2B5697F795489C8710BCC72141FF602F765C@ex07.net.ucsf.edu> Yes, Not to be confused with the AutoSection, which is essential a self-aligning microtome The SmartSection uses a 5-axis robot (think of one of those robotic arms) to move blocks and slides around a station for sectioning. It picks a block from a rack and places the block in a holder to do the sectioning, mounts the sections on slides and puts in rack. Can detect tissue in the block, face correctly to full face, check for holes in the section (all imaging) and do multiple sections per slide, do levels at programmed depths, etc. I guess there is not anything online about it. Sakura demoed it at NSH last year to select customers, and not at the general trade show. Tim -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis at baylorhealth.edu] Sent: Friday, July 10, 2015 1:47 PM To: 'Cooper, Brian'; Morken, Timothy; histonet at lists.utsouthwestern.edu Subject: RE: Sakura SmartSection Brian, I think the unit that Tim is talking about is something different. His unit has not need for a tech to pick up sections. The sections are placed on the slide by the robot. We are currently demoing the new Sakura Tissue Tek Auto Section Microtome that does not use a robot. I will have to say that if it is the same unit that you looked at years ago, they have worked out the bugs. It is a really nice unit. I technicians do not want the unit to leave when the demo is complete. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com -----Original Message----- From: Cooper, Brian [mailto:bcooper at chla.usc.edu] Sent: Friday, July 10, 2015 3:00 PM To: Morken, Timothy; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Sakura SmartSection I went to Sakura to see that thing on a demo a few years back, and it was . . . scary! The sales reps couldn't get it calibrated right, and it ripped tissue out of the blocks while it was trimming on a few occasions. Not one of us in the room (pretty much all experienced bench histotechs) could collect ribbons, though I suspect this could have been a learning curve type thing. Aside from that, I will say that they had the ability to realign blocks pretty well perfected, and I was impressed by that. I went back to Sakura about a year later for something else, and our rep was still shaking her head about how bad that demo experience went! Sounds like they may have worked out the kinks eh? Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Friday, July 10, 2015 12:26 PM To: Histonet Subject: [Histonet] Sakura SmartSection Has anyone done any work with the Sakura SmartSection robot? We've had some blocks cut on it and have had good initial results. This could be a game-changer for histology staffing. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=bKZPIq7CMd5nofh_6PpWlhPjBnWHJcPszndK-_NoX0s&s=nMwE-aXCRu2omFYsrK30C0y2SkmCo8U3KR2uQuV5uos&e= --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=bKZPIq7CMd5nofh_6PpWlhPjBnWHJcPszndK-_NoX0s&s=nMwE-aXCRu2omFYsrK30C0y2SkmCo8U3KR2uQuV5uos&e= ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From Donna.Willis at baylorhealth.edu Fri Jul 10 17:05:43 2015 From: Donna.Willis at baylorhealth.edu (Willis, Donna G.) Date: Fri, 10 Jul 2015 22:05:43 +0000 Subject: [Histonet] Sakura SmartSection In-Reply-To: <761E2B5697F795489C8710BCC72141FF602F765C@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF602F7596@ex07.net.ucsf.edu> <2572B4D63B62E64A8078D8BBE34D40788DB759C7@BHDASVEXML2.bhcs.pvt>, <761E2B5697F795489C8710BCC72141FF602F765C@ex07.net.ucsf.edu> Message-ID: <59F64508-FDB5-4F0E-AD68-F3FF1739A948@baylorhealth.edu> I wish it was released. Purchasing new microtones this year. Sent from my iPhone > On Jul 10, 2015, at 4:56 PM, Morken, Timothy wrote: > > Yes, Not to be confused with the AutoSection, which is essential a self-aligning microtome > > The SmartSection uses a 5-axis robot (think of one of those robotic arms) to move blocks and slides around a station for sectioning. It picks a block from a rack and places the block in a holder to do the sectioning, mounts the sections on slides and puts in rack. Can detect tissue in the block, face correctly to full face, check for holes in the section (all imaging) and do multiple sections per slide, do levels at programmed depths, etc. > > I guess there is not anything online about it. Sakura demoed it at NSH last year to select customers, and not at the general trade show. > > Tim > > -----Original Message----- > From: Willis, Donna G. [mailto:Donna.Willis at baylorhealth.edu] > Sent: Friday, July 10, 2015 1:47 PM > To: 'Cooper, Brian'; Morken, Timothy; histonet at lists.utsouthwestern.edu > Subject: RE: Sakura SmartSection > > Brian, > I think the unit that Tim is talking about is something different. His unit has not need for a tech to pick up sections. The sections are placed on the slide by the robot. > > We are currently demoing the new Sakura Tissue Tek Auto Section Microtome that does not use a robot. I will have to say that if it is the same unit that you looked at years ago, they have worked out the bugs. It is a really nice unit. I technicians do not want the unit to leave when the demo is complete. > > Donna Willis, HT/HTL(ASCP) > Anatomic Pathology Manager > > Baylor University Medical Center > 3500 Gaston Ave|Dallas, Texas 75246 > 214-820-2465 office|214-725-6184 mobile > BaylorScottandWhite.com > > > > -----Original Message----- > From: Cooper, Brian [mailto:bcooper at chla.usc.edu] > Sent: Friday, July 10, 2015 3:00 PM > To: Morken, Timothy; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Sakura SmartSection > > I went to Sakura to see that thing on a demo a few years back, and it was . . . scary! The sales reps couldn't get it calibrated right, and it ripped tissue out of the blocks while it was trimming on a few occasions. Not one of us in the room (pretty much all experienced bench histotechs) could collect ribbons, though I suspect this could have been a learning curve type thing. Aside from that, I will say that they had the ability to realign blocks pretty well perfected, and I was impressed by that. > > I went back to Sakura about a year later for something else, and our rep was still shaking her head about how bad that demo experience went! Sounds like they may have worked out the kinks eh? > > Thanks, > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 > bcooper at chla.usc.edu > > > -----Original Message----- > From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] > Sent: Friday, July 10, 2015 12:26 PM > To: Histonet > Subject: [Histonet] Sakura SmartSection > > Has anyone done any work with the Sakura SmartSection robot? We've had some blocks cut on it and have had good initial results. This could be a game-changer for histology staffing. > > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=bKZPIq7CMd5nofh_6PpWlhPjBnWHJcPszndK-_NoX0s&s=nMwE-aXCRu2omFYsrK30C0y2SkmCo8U3KR2uQuV5uos&e= > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=bKZPIq7CMd5nofh_6PpWlhPjBnWHJcPszndK-_NoX0s&s=nMwE-aXCRu2omFYsrK30C0y2SkmCo8U3KR2uQuV5uos&e= > > ********************************************************************** > This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From j.rowaihi at alborglaboratories.com Fri Jul 10 17:22:01 2015 From: j.rowaihi at alborglaboratories.com (j.rowaihi) Date: Sat, 11 Jul 2015 01:22:01 +0300 Subject: [Histonet] Sakura SmartSection Message-ID: I agree with you?Donna but we can't deny the support of the robots and the automation which help much and give a fantastic job with less time even in the most special technique in histopath. Regards Jamal Rowaihi Anatomic Pathology Supervisor Al Borg Medical Laboratories *Sent from my cell phoner* -------- Original message -------- From: "Willis, Donna G." Date: 07/11/2015 12:46 AM (GMT+03:00) To: "'Cooper, Brian'" ,"Morken, Timothy" ,histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Sakura SmartSection I have seen more issues from the human side than automation.? Currently all instruments on the market have to still have a technician assist with the microtomy. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas? 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com -----Original Message----- From: Cooper, Brian [mailto:bcooper at chla.usc.edu] Sent: Friday, July 10, 2015 4:40 PM To: Willis, Donna G.; Morken, Timothy; histonet at lists.utsouthwestern.edu Subject: RE: Sakura SmartSection Ah!? I stand corrected.? I have to admit, I'm really leery about letting automated microtomes be in the same vicinity of renal or liver needle biopsies--are you guys comfortable with this??? Brian -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis at baylorhealth.edu] Sent: Friday, July 10, 2015 1:47 PM To: Cooper, Brian; Morken, Timothy; histonet at lists.utsouthwestern.edu Subject: RE: Sakura SmartSection Brian, I think the unit that Tim is talking about is something different.? His unit has not need for a tech to pick up sections.? The sections are placed on the slide by the robot.? We are currently demoing the new Sakura Tissue Tek Auto Section Microtome that does not use a robot.? I will have to say that if it is the same unit that you looked at years ago, they have worked out the bugs.? It is a really nice unit.? I technicians do not want the unit to leave when the demo is complete. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas? 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com -----Original Message----- From: Cooper, Brian [mailto:bcooper at chla.usc.edu] Sent: Friday, July 10, 2015 3:00 PM To: Morken, Timothy; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Sakura SmartSection I went to Sakura to see that thing on a demo a few years back, and it was . . . scary!? The sales reps couldn't get it calibrated right, and it ripped tissue out of the blocks while it was trimming on a few occasions. Not one of us in the room (pretty much all experienced bench histotechs) could collect ribbons, though I suspect this could have been a learning curve type thing.? Aside from that, I will say that they had the ability to realign blocks pretty well perfected, and I was impressed by that.? I went back to Sakura about a year later for something else, and our rep was still shaking her head about how bad that demo experience went!?? Sounds like they may have worked out the kinks eh???? Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Friday, July 10, 2015 12:26 PM To: Histonet Subject: [Histonet] Sakura SmartSection Has anyone done any work with the Sakura SmartSection robot? We've had some blocks cut on it and have had good initial results. This could be a game-changer for histology staffing. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=bKZPIq7CMd5nofh_6PpWlhPjBnWHJcPszndK-_NoX0s&s=nMwE-aXCRu2omFYsrK30C0y2SkmCo8U3KR2uQuV5uos&e= --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message.? --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=bKZPIq7CMd5nofh_6PpWlhPjBnWHJcPszndK-_NoX0s&s=nMwE-aXCRu2omFYsrK30C0y2SkmCo8U3KR2uQuV5uos&e= ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message.? --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan at uwo.ca Sat Jul 11 00:55:28 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Sat, 11 Jul 2015 00:55:28 -0500 Subject: [Histonet] Coverslipping mystery In-Reply-To: <73c0d1b2a289.55a0af4c@uwo.ca> References: <73c0f878cfab.55a0a8cf@uwo.ca> <7360f962f7ac.55a0a9bf@uwo.ca> <7360e07ed582.55a0a9fd@uwo.ca> <7360a115b931.55a0aa3c@uwo.ca> <7300d1e8a856.55a0aa7a@uwo.ca> <7360cb6ca499.55a0aab8@uwo.ca> <73609945f5a7.55a0aaf6@uwo.ca> <73608461f61e.55a0ab34@uwo.ca> <7310f7c1eea9.55a0ab72@uwo.ca> <73c0f258e785.55a0abb1@uwo.ca> <7350f85f96b7.55a0abef@uwo.ca> <73009c3e9bc2.55a0ac2d@uwo.ca> <72d0ba59d987.55a0ac6c@uwo.ca> <73209fa9e809.55a0acaa@uwo.ca> <7320e8fd84b1.55a0ace8@uwo.ca> <73c0b2cfcc04.55a0ad26@uwo.ca> <7310a2dedc5e.55a0ad64@uwo.ca> <7310d04e852a.55a0ada2@uwo.ca> <7310a1bfcbbf.55a0ade0@uwo.ca> <7310c29aa831.55a0ae1e@uwo.ca> <7360f353b844.55a0ae99@uwo.ca> <7340d3a7d20b.55a0aed7@uwo.ca> <7340a9fbff76.55a0af15@uwo.ca> <73c0d1b2a289.55a0af4c@uwo.ca> Message-ID: <73c0d9cedd62.55a06980@uwo.ca> DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. John Kiernan = = = On 09/07/15, Adam Boanas wrote: > Hello, > > We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! > ?en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? > We are struggling for ideas with this one! - this occurs with fresh DPX also. > > Many thanks > Adam > > Adam Boanas > Senior Research Associate > Epistem Ltd > 48 Grafton Street > Manchester, M13 9XX > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From mills at 3scan.com Sat Jul 11 09:17:30 2015 From: mills at 3scan.com (Caroline Miller) Date: Sat, 11 Jul 2015 07:17:30 -0700 Subject: [Histonet] Coverslipping mystery In-Reply-To: <73c0d9cedd62.55a06980@uwo.ca> References: <73c0f878cfab.55a0a8cf@uwo.ca> <7360f962f7ac.55a0a9bf@uwo.ca> <7360e07ed582.55a0a9fd@uwo.ca> <7360a115b931.55a0aa3c@uwo.ca> <7300d1e8a856.55a0aa7a@uwo.ca> <7360cb6ca499.55a0aab8@uwo.ca> <73609945f5a7.55a0aaf6@uwo.ca> <73608461f61e.55a0ab34@uwo.ca> <7310f7c1eea9.55a0ab72@uwo.ca> <73c0f258e785.55a0abb1@uwo.ca> <7350f85f96b7.55a0abef@uwo.ca> <73009c3e9bc2.55a0ac2d@uwo.ca> <72d0ba59d987.55a0ac6c@uwo.ca> <73209fa9e809.55a0acaa@uwo.ca> <7320e8fd84b1.55a0ace8@uwo.ca> <73c0b2cfcc04.55a0ad26@uwo.ca> <7310a2dedc5e.55a0ad64@uwo.ca> <7310d04e852a.55a0ada2@uwo.ca> <7310a1bfcbbf.55a0ade0@uwo.ca> <7310c29aa831.55a0ae1e@uwo.ca> <7360f353b844.55a0ae99@uwo.ca> <7340d3a7d20b.55a0aed7@uwo.ca> <7340a9fbff76.55a0af15@uwo.ca> <73c0d1b2a289.55a0af4c@uwo.ca> <73c0d9cedd62.55a06980@uwo.ca> Message-ID: <6C960C7A-BC93-463A-A408-2A94B7918650@3scan.com> I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time..... When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something! Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 > On Jul 10, 2015, at 10:55 PM, John Kiernan wrote: > > DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. > > In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. > > Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. > > John Kiernan > = = = >> On 09/07/15, Adam Boanas wrote: >> Hello, >> >> We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! >> en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? >> We are struggling for ideas with this one! - this occurs with fresh DPX also. >> >> Many thanks >> Adam >> >> Adam Boanas >> Senior Research Associate >> Epistem Ltd >> 48 Grafton Street >> Manchester, M13 9XX >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From regina.vontell at kcl.ac.uk Sun Jul 12 10:21:01 2015 From: regina.vontell at kcl.ac.uk (Vontell, Regina) Date: Sun, 12 Jul 2015 15:21:01 +0000 Subject: [Histonet] 1. Re: Coverslipping mystery (Caroline Miller) In-Reply-To: References: Message-ID: 1. Re: Coverslipping mystery I agree with Carol, my lab uses DPX and I have not had a problem with the product drying out. I do notice that if air gets trapped in the viscous solution there is a potential for the coverslips to fall off or have large air pockets. We use wooden chopsticks to apply the DPX to the slide. I find this works really well and it reduces the amount of air bubbles that form with using a plastic squeeze pipette. Regina ________________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: 11 July 2015 18:00 To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 140, Issue 12 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Coverslipping mystery (Caroline Miller) ---------------------------------------------------------------------- Message: 1 Date: Sat, 11 Jul 2015 07:17:30 -0700 From: Caroline Miller To: John Kiernan Cc: Adam Boanas , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Coverslipping mystery Message-ID: <6C960C7A-BC93-463A-A408-2A94B7918650 at 3scan.com> Content-Type: text/plain; charset=us-ascii I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time..... When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something! Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 > On Jul 10, 2015, at 10:55 PM, John Kiernan wrote: > > DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. > > In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. > > Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. > > John Kiernan > = = = >> On 09/07/15, Adam Boanas wrote: >> Hello, >> >> We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! >> en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? >> We are struggling for ideas with this one! - this occurs with fresh DPX also. >> >> Many thanks >> Adam >> >> Adam Boanas >> Senior Research Associate >> Epistem Ltd >> 48 Grafton Street >> Manchester, M13 9XX >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 140, Issue 12 ***************************************** From jqb7 at cdc.gov Sun Jul 12 13:23:05 2015 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Sun, 12 Jul 2015 18:23:05 +0000 Subject: [Histonet] Sakura SmartSection In-Reply-To: <761E2B5697F795489C8710BCC72141FF602F7596@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF602F7596@ex07.net.ucsf.edu> Message-ID: <3B2CD438E1628A41BD687E98B963B78137F75E64@EMBX-CLFT4.cdc.gov> Can't wait to try it! We have 3 Autosections and love them! -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Friday, July 10, 2015 3:26 PM To: Histonet Subject: [Histonet] Sakura SmartSection Has anyone done any work with the Sakura SmartSection robot? We've had some blocks cut on it and have had good initial results. This could be a game-changer for histology staffing. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 at cdc.gov Sun Jul 12 13:26:16 2015 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Sun, 12 Jul 2015 18:26:16 +0000 Subject: [Histonet] Sakura SmartSection In-Reply-To: <2572B4D63B62E64A8078D8BBE34D40788DB759C7@BHDASVEXML2.bhcs.pvt> References: <761E2B5697F795489C8710BCC72141FF602F7596@ex07.net.ucsf.edu> <2572B4D63B62E64A8078D8BBE34D40788DB759C7@BHDASVEXML2.bhcs.pvt> Message-ID: <3B2CD438E1628A41BD687E98B963B78137F77EC2@EMBX-CLFT4.cdc.gov> We have 3 and LOVE them! -----Original Message----- From: Willis, Donna G. [mailto:Donna.Willis at baylorhealth.edu] Sent: Friday, July 10, 2015 4:47 PM To: 'Cooper, Brian'; Morken, Timothy; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Sakura SmartSection Brian, I think the unit that Tim is talking about is something different. His unit has not need for a tech to pick up sections. The sections are placed on the slide by the robot. We are currently demoing the new Sakura Tissue Tek Auto Section Microtome that does not use a robot. I will have to say that if it is the same unit that you looked at years ago, they have worked out the bugs. It is a really nice unit. I technicians do not want the unit to leave when the demo is complete. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com -----Original Message----- From: Cooper, Brian [mailto:bcooper at chla.usc.edu] Sent: Friday, July 10, 2015 3:00 PM To: Morken, Timothy; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Sakura SmartSection I went to Sakura to see that thing on a demo a few years back, and it was . . . scary! The sales reps couldn't get it calibrated right, and it ripped tissue out of the blocks while it was trimming on a few occasions. Not one of us in the room (pretty much all experienced bench histotechs) could collect ribbons, though I suspect this could have been a learning curve type thing. Aside from that, I will say that they had the ability to realign blocks pretty well perfected, and I was impressed by that. I went back to Sakura about a year later for something else, and our rep was still shaking her head about how bad that demo experience went! Sounds like they may have worked out the kinks eh? Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Friday, July 10, 2015 12:26 PM To: Histonet Subject: [Histonet] Sakura SmartSection Has anyone done any work with the Sakura SmartSection robot? We've had some blocks cut on it and have had good initial results. This could be a game-changer for histology staffing. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAw&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=bKZPIq7CMd5nofh_6PpWlhPjBnWHJcPszndK-_NoX0s&s=nMwE-aXCRu2omFYsrK30C0y2SkmCo8U3KR2uQuV5uos&e= --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. 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Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.boanas at epistem.co.uk Mon Jul 13 02:30:44 2015 From: a.boanas at epistem.co.uk (Adam Boanas) Date: Mon, 13 Jul 2015 07:30:44 +0000 Subject: [Histonet] Coverslipping mystery In-Reply-To: <6C960C7A-BC93-463A-A408-2A94B7918650@3scan.com> References: <73c0f878cfab.55a0a8cf@uwo.ca> <7360f962f7ac.55a0a9bf@uwo.ca> <7360e07ed582.55a0a9fd@uwo.ca> <7360a115b931.55a0aa3c@uwo.ca> <7300d1e8a856.55a0aa7a@uwo.ca> <7360cb6ca499.55a0aab8@uwo.ca> <73609945f5a7.55a0aaf6@uwo.ca> <73608461f61e.55a0ab34@uwo.ca> <7310f7c1eea9.55a0ab72@uwo.ca> <73c0f258e785.55a0abb1@uwo.ca> <7350f85f96b7.55a0abef@uwo.ca> <73009c3e9bc2.55a0ac2d@uwo.ca> <72d0ba59d987.55a0ac6c@uwo.ca> <73209fa9e809.55a0acaa@uwo.ca> <7320e8fd84b1.55a0ace8@uwo.ca> <73c0b2cfcc04.55a0ad26@uwo.ca> <7310a2dedc5e.55a0ad64@uwo.ca> <7310d04e852a.55a0ada2@uwo.ca> <7310a1bfcbbf.55a0ade0@uwo.ca> <7310c29aa831.55a0ae1e@uwo.ca> <7360f353b844.55a0ae99@uwo.ca> <7340d3a7d20b.55a0aed7@uwo.ca> <7340a9fbff76.55a0af15@uwo.ca> <73c0d1b2a289.55a0af4c@uwo.ca> <73c0d9cedd62.55a06980@uwo.ca> <6C960C7A-BC93-463A-A408-2A94B7918650@3scan.com> Message-ID: Hello again, Thought I might offer an update on the coverslipping issue as it might be of use in future. I ran a test last week of manual coverslipping using blank charged and un-charged slides and using DPX and Pertex as the mountant. I also used 2 methods of application. 1) Mountant applied using plastic Pasteur pipette 2) Mountant applied using aluminium screw cap tube. Following immersion in xylene for 5 mins the coverslips were applied. From viewing this morning, all slides were clear with the exception of those coverslipped using DPX applied with the Pasteur. In each case, these slides had the 'parched earth' artefact having been left to dry over the weekend. I suspect that the DPX has had a reaction with the plastic of the pipette during application and the artefact is caused by residual `molten` plastic from the pipette that only reveals itself over time. Does this sound plausible? No problem with the pertex and pipettes (which is what I've used for years with no issue) Thanks Adam -----Original Message----- From: Caroline Miller [mailto:mills at 3scan.com] Sent: 11 July 2015 15:18 To: John Kiernan Cc: Adam Boanas; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Coverslipping mystery I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time..... When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something! Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 > On Jul 10, 2015, at 10:55 PM, John Kiernan wrote: > > DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. > > In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. > > Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. > > John Kiernan > = = = >> On 09/07/15, Adam Boanas wrote: >> Hello, >> >> We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! >> en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? >> We are struggling for ideas with this one! - this occurs with fresh DPX also. >> >> Many thanks >> Adam >> >> Adam Boanas >> Senior Research Associate >> Epistem Ltd >> 48 Grafton Street >> Manchester, M13 9XX >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Deborah.Rogers at hattiesburgclinic.com Mon Jul 13 06:41:55 2015 From: Deborah.Rogers at hattiesburgclinic.com (Deborah.Rogers at hattiesburgclinic.com) Date: Mon, 13 Jul 2015 11:41:55 +0000 Subject: [Histonet] Histonet Digest, Vol 140, Issue 12 In-Reply-To: References: Message-ID: <532531A59A9E314891756C7AA4B001E3015A3105EA@VMMX02.hattiesburgclinic.com> I'm studying for the ASCP Safety Qualification. Looking for advice from an experienced source. Thanks! ________________________________________ From: histonet-request at lists.utsouthwestern.edu [histonet-request at lists.utsouthwestern.edu] Sent: Saturday, July 11, 2015 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 140, Issue 12 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Coverslipping mystery (Caroline Miller) ---------------------------------------------------------------------- Message: 1 Date: Sat, 11 Jul 2015 07:17:30 -0700 From: Caroline Miller To: John Kiernan Cc: Adam Boanas , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Coverslipping mystery Message-ID: <6C960C7A-BC93-463A-A408-2A94B7918650 at 3scan.com> Content-Type: text/plain; charset=us-ascii I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time..... When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something! Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 > On Jul 10, 2015, at 10:55 PM, John Kiernan wrote: > > DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. > > In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. > > Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. > > John Kiernan > = = = >> On 09/07/15, Adam Boanas wrote: >> Hello, >> >> We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! >> en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? >> We are struggling for ideas with this one! - this occurs with fresh DPX also. >> >> Many thanks >> Adam >> >> Adam Boanas >> Senior Research Associate >> Epistem Ltd >> 48 Grafton Street >> Manchester, M13 9XX >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 140, Issue 12 ***************************************** ------------------------------------------------------------------------- This message was secured by ZixCorp(R). From b-frederick at northwestern.edu Mon Jul 13 07:41:42 2015 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Mon, 13 Jul 2015 12:41:42 +0000 Subject: [Histonet] Coverslipping mystery In-Reply-To: <6C960C7A-BC93-463A-A408-2A94B7918650@3scan.com> References: <73c0f878cfab.55a0a8cf@uwo.ca> <7360f962f7ac.55a0a9bf@uwo.ca> <7360e07ed582.55a0a9fd@uwo.ca> <7360a115b931.55a0aa3c@uwo.ca> <7300d1e8a856.55a0aa7a@uwo.ca> <7360cb6ca499.55a0aab8@uwo.ca> <73609945f5a7.55a0aaf6@uwo.ca> <73608461f61e.55a0ab34@uwo.ca> <7310f7c1eea9.55a0ab72@uwo.ca> <73c0f258e785.55a0abb1@uwo.ca> <7350f85f96b7.55a0abef@uwo.ca> <73009c3e9bc2.55a0ac2d@uwo.ca> <72d0ba59d987.55a0ac6c@uwo.ca> <73209fa9e809.55a0acaa@uwo.ca> <7320e8fd84b1.55a0ace8@uwo.ca> <73c0b2cfcc04.55a0ad26@uwo.ca> <7310a2dedc5e.55a0ad64@uwo.ca> <7310d04e852a.55a0ada2@uwo.ca> <7310a1bfcbbf.55a0ade0@uwo.ca> <7310c29aa831.55a0ae1e@uwo.ca> <7360f353b844.55a0ae99@uwo.ca> <7340d3a7d20b.55a0aed7@uwo.ca> <7340a9fbff76.55a0af15@uwo.ca> <73c0d1b2a289.55a0af4c@uwo.ca> <73c0d9cedd62.55a06980@uwo.ca> <6C960C7A-BC93-463A-A408-2A94B7918650@3scan.com> Message-ID: We have a CV5030 and use the suggested Surgipath micromount. It works well. As has been previously mentioned, you can adjust the amount of mountant dispensed as desired. Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu -----Original Message----- From: Caroline Miller [mailto:mills at 3scan.com] Sent: Saturday, July 11, 2015 9:18 AM To: John Kiernan Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Coverslipping mystery I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time..... When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something! Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 > On Jul 10, 2015, at 10:55 PM, John Kiernan wrote: > > DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. > > In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. > > Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. > > John Kiernan > = = = >> On 09/07/15, Adam Boanas wrote: >> Hello, >> >> We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! >> en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? >> We are struggling for ideas with this one! - this occurs with fresh DPX also. >> >> Many thanks >> Adam >> >> Adam Boanas >> Senior Research Associate >> Epistem Ltd >> 48 Grafton Street >> Manchester, M13 9XX >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mills at 3scan.com Mon Jul 13 08:32:37 2015 From: mills at 3scan.com (Caroline Miller) Date: Mon, 13 Jul 2015 06:32:37 -0700 Subject: [Histonet] Coverslipping mystery In-Reply-To: References: <73c0f878cfab.55a0a8cf@uwo.ca> <7360f962f7ac.55a0a9bf@uwo.ca> <7360e07ed582.55a0a9fd@uwo.ca> <7360a115b931.55a0aa3c@uwo.ca> <7300d1e8a856.55a0aa7a@uwo.ca> <7360cb6ca499.55a0aab8@uwo.ca> <73609945f5a7.55a0aaf6@uwo.ca> <73608461f61e.55a0ab34@uwo.ca> <7310f7c1eea9.55a0ab72@uwo.ca> <73c0f258e785.55a0abb1@uwo.ca> <7350f85f96b7.55a0abef@uwo.ca> <73009c3e9bc2.55a0ac2d@uwo.ca> <72d0ba59d987.55a0ac6c@uwo.ca> <73209fa9e809.55a0acaa@uwo.ca> <7320e8fd84b1.55a0ace8@uwo.ca> <73c0b2cfcc04.55a0ad26@uwo.ca> <7310a2dedc5e.55a0ad64@uwo.ca> <7310d04e852a.55a0ada2@uwo.ca> <7310a1bfcbbf.55a0ade0@uwo.ca> <7310c29aa831.55a0ae1e@uwo.ca> <7360f353b844.55a0ae99@uwo.ca> <7340d3a7d20b.55a0aed7@uwo.ca> <7340a9fbff76.55a0af15@uwo.ca> <73c0d1b2a289.55a0af4c@uwo.ca> <73c0d9cedd62.55a06980@uwo.ca> <6C960C7A-BC93-463A-A408-2A94B7918650@3scan.com> Message-ID: It sounds totally plausible, but just to put a spanner in the works; I have a plastic pipette in my DPX that I leave in there for at least 6 months and I don't get that artifact. We may have different pipettess though. Yours, mills Caroline Miller (mills) Director of Histology 3Scan, Inc 415-2187297 > On Jul 13, 2015, at 12:30 AM, Adam Boanas wrote: > > Hello again, > > Thought I might offer an update on the coverslipping issue as it might be of use in future. > I ran a test last week of manual coverslipping using blank charged and un-charged slides and using DPX and Pertex as the mountant. I also used 2 methods of application. > 1) Mountant applied using plastic Pasteur pipette > 2) Mountant applied using aluminium screw cap tube. > Following immersion in xylene for 5 mins the coverslips were applied. From viewing this morning, all slides were clear with the exception of those coverslipped using DPX applied with the Pasteur. In each case, these slides had the 'parched earth' artefact having been left to dry over the weekend. > I suspect that the DPX has had a reaction with the plastic of the pipette during application and the artefact is caused by residual `molten` plastic from the pipette that only reveals itself over time. > Does this sound plausible? No problem with the pertex and pipettes (which is what I've used for years with no issue) > Thanks > Adam > > -----Original Message----- > From: Caroline Miller [mailto:mills at 3scan.com] > Sent: 11 July 2015 15:18 > To: John Kiernan > Cc: Adam Boanas; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Coverslipping mystery > > I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time..... > > When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something! > > Yours, > mills > > Caroline Miller (mills) > Director of Histology > 3Scan, Inc > 415-2187297 > >> On Jul 10, 2015, at 10:55 PM, John Kiernan wrote: >> >> DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media. >> >> In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant. >> >> Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips. >> >> John Kiernan >> = = = >>> On 09/07/15, Adam Boanas wrote: >>> Hello, >>> >>> We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh! >>> en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it? >>> We are struggling for ideas with this one! - this occurs with fresh DPX also. >>> >>> Many thanks >>> Adam >>> >>> Adam Boanas >>> Senior Research Associate >>> Epistem Ltd >>> 48 Grafton Street >>> Manchester, M13 9XX >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Reuel.Cornelia at tsrh.org Mon Jul 13 08:49:17 2015 From: Reuel.Cornelia at tsrh.org (Reuel Cornelia) Date: Mon, 13 Jul 2015 08:49:17 -0500 Subject: [Histonet] Non Specific Esterase Message-ID: <55A37B8D020000C50002C7FA@mail.tsrh.org> Hello Histonetters, Does anyone have a protocol for NSE on paraffin sections? Reuel From fourfonners at yahoo.com Mon Jul 13 09:28:21 2015 From: fourfonners at yahoo.com (Sheila Fonner) Date: Mon, 13 Jul 2015 14:28:21 +0000 (UTC) Subject: [Histonet] Looking for someone who can do this stain for us Message-ID: <939557060.604121.1436797701668.JavaMail.yahoo@mail.yahoo.com> Hello Histonetters, I need your help. I have a doc looking for someone to perform an IHC stain for us. It's a p41/38 moab antibody for HHV-6. If anyone knows of someone doing this stain who also accepts outside cases, I would really appreciate the contact info. Thank you and have a wonderful Monday. SheilaDPPKnoxville, TN From Valerie.Hannen at parrishmed.com Mon Jul 13 10:05:21 2015 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Mon, 13 Jul 2015 11:05:21 -0400 Subject: [Histonet] Eosin Message-ID: <450B7A81EDA0C54E97C53D60F00776C32337384270@isexstore03> Good morning, I once again am dealing with a my picky Pathologist!! About a year ago, he started to complain about not having enough Eosin on his sections( first morning rack on Monday).. I went from changing the Eosin on Friday and stirring it on Monday to totally changing it on Monday...it has all been good until the end of last week. The problem has started up again... little Eosin in the first rack of Monday morning. Any suggestions?? Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From rjbuesa at yahoo.com Mon Jul 13 10:19:58 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 13 Jul 2015 15:19:58 +0000 (UTC) Subject: [Histonet] Eosin In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C32337384270@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C32337384270@isexstore03> Message-ID: <1199340431.1163732.1436800798302.JavaMail.yahoo@mail.yahoo.com> It is difficult to advise you anything without knowing what is your PT's complaint. What he does complain about? Ren? On Monday, July 13, 2015 11:17 AM, "Hannen, Valerie" wrote: Good morning, I once again am dealing with a my picky Pathologist!!? About a year ago, he started to complain about not having enough Eosin on his sections( first morning rack on Monday).. I went from changing the Eosin on Friday and stirring it on Monday to totally changing it? on Monday...it has all been good until the end of last week. The problem has started up again... little Eosin in the first rack of Monday morning.? Any suggestions?? Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kkienitz at orclinic.com Mon Jul 13 10:20:46 2015 From: kkienitz at orclinic.com (Kienitz, Kari) Date: Mon, 13 Jul 2015 08:20:46 -0700 Subject: [Histonet] Eosin In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C32337384270@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C32337384270@isexstore03> Message-ID: <41400FFE517878449D89114DD2526090196602D1BB@tocmail1.tocad.orclinic.com> It's always kind of a stab in the dark to diagnose H&E problems without a little more information. One thing I would try if you don't feel its an actual Eosin problem is your alcohol prior to the eosin. Slide volume and humidity can dilute your alcohol and cause lighter cytoplasmic staining. The alcohol prior to eosin should be 95% and changed daily if need be. Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz at orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: Hannen, Valerie [Valerie.Hannen at parrishmed.com] Sent: Monday, July 13, 2015 8:05 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Eosin Good morning, I once again am dealing with a my picky Pathologist!! About a year ago, he started to complain about not having enough Eosin on his sections( first morning rack on Monday).. I went from changing the Eosin on Friday and stirring it on Monday to totally changing it on Monday...it has all been good until the end of last week. The problem has started up again... little Eosin in the first rack of Monday morning. Any suggestions?? Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Mon Jul 13 10:35:24 2015 From: patpxs at gmail.com (Paula Sicurello) Date: Mon, 13 Jul 2015 08:35:24 -0700 Subject: [Histonet] Source for ultra low temperature immersion thermometer Message-ID: Good Morning Histolanders, Does anyone have a source for an immersion thermometer that can be used to measure the temperature of the isopentane or 2-methylbutane or methylbutane ( you choose) ;-) used for freezing muscle biopsies? I have searched using Dr. Google and cannot find one that goes below -100 degrees C. Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UCSD Medical Center 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From Timothy.Morken at ucsf.edu Mon Jul 13 11:10:23 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 13 Jul 2015 16:10:23 +0000 Subject: [Histonet] Source for ultra low temperature immersion thermometer In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF602F7935@ex07.net.ucsf.edu> Paula, we use an Oakton Temp10K with a Type-K thermocouple probe (goes to -250C) . We got it from Fisher scientific https://www.fishersci.com/shop/products/oakton-digi-sense-dual-input-j-k-t-thermocouple-thermometer/p-203772 Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Paula Sicurello [mailto:patpxs at gmail.com] Sent: Monday, July 13, 2015 8:35 AM To: HistoNet Subject: [Histonet] Source for ultra low temperature immersion thermometer Good Morning Histolanders, Does anyone have a source for an immersion thermometer that can be used to measure the temperature of the isopentane or 2-methylbutane or methylbutane ( you choose) ;-) used for freezing muscle biopsies? I have searched using Dr. Google and cannot find one that goes below -100 degrees C. Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UCSD Medical Center 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abadesuyi at nrh-ok.com Mon Jul 13 11:52:29 2015 From: abadesuyi at nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Mon, 13 Jul 2015 11:52:29 -0500 Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories Message-ID: <04EE4F75BB5FB246ADB68D69B7460443A37D48807E@MAIL.nrhnt.nrh-ok.com> Hi, I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning. I wanted to know whether some of you guys out there are using Formaldehyde substitute. Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 abadesuyi at nrh-ok.com ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From Valerie.Hannen at parrishmed.com Mon Jul 13 12:30:50 2015 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Mon, 13 Jul 2015 13:30:50 -0400 Subject: [Histonet] Eosin In-Reply-To: <1199340431.1163732.1436800798302.JavaMail.yahoo@mail.yahoo.com> References: <450B7A81EDA0C54E97C53D60F00776C32337384270@isexstore03> <1199340431.1163732.1436800798302.JavaMail.yahoo@mail.yahoo.com> Message-ID: <450B7A81EDA0C54E97C53D60F00776C32337384272@isexstore03> Rene, As I stated before and maybe I was not entirely clear, we had this problem ( little Eosin in the sections of the first rack of slides on Monday morning) before, but had remedied it by starting to change the Eosin on Monday morning before the first rack of slides was run. He now is stating that the problem of little Eosin in the sections from the first rack on Monday A.M. is starting again. Since the last fix of the problem we have not deviated in any way, procedure or chemical wise. We have used the same lot # in previous weeks for the Eosin as we currently have on the stainer ( I changed the Eosin this morning) , so I don?t think it is an Eosin problem per se. Kari, We do use 95% alcohol before our Eosin at all times. I would think that if the alcohol before the Eosin was the problem, all of my racks would have the ?little Eosin in the sections? problem.. not just the first rack. Again any and all suggestions are welcomed!! Thanks again Rene and Kari for replying. Valerie From: Rene J Buesa [mailto:rjbuesa at yahoo.com] Sent: Monday, July 13, 2015 11:20 AM To: Hannen, Valerie; Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Eosin It is difficult to advise you anything without knowing what is your PT's complaint. What he does complain about? Ren? On Monday, July 13, 2015 11:17 AM, "Hannen, Valerie" > wrote: Good morning, I once again am dealing with a my picky Pathologist!! About a year ago, he started to complain about not having enough Eosin on his sections( first morning rack on Monday).. I went from changing the Eosin on Friday and stirring it on Monday to totally changing it on Monday...it has all been good until the end of last week. The problem has started up again... little Eosin in the first rack of Monday morning. Any suggestions?? Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com> www.parrishmed.com ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From susanhay59 at gmail.com Mon Jul 13 12:39:11 2015 From: susanhay59 at gmail.com (S hay) Date: Mon, 13 Jul 2015 12:39:11 -0500 Subject: [Histonet] Pax8 Message-ID: Is anyone using pax 8 with iview detection on the Ventana Ultra? From rjbuesa at yahoo.com Mon Jul 13 13:04:43 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 13 Jul 2015 18:04:43 +0000 (UTC) Subject: [Histonet] Eosin In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C32337384272@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C32337384272@isexstore03> Message-ID: <2120782795.1297443.1436810683042.JavaMail.yahoo@mail.yahoo.com> Sometimes these issues are "mysterious" and in your case are particularly "mysterious" because you once solved the problem, only to recently?"reappear".I am going to tell you how I used to do it and never had those PT rejections:1- for both hematoxylin and eosin?I kept a log of the number of stained slides and changes both when I reached 200, regardless of the day of the week or the time within the day the amount was reached.2- I always used the same brand (Harleco) 3- I never left the staining set over the week. Everything was removed the last day of work (Saturday) and the containers were rinsed and dried and left upside down. For the automatic stainer (Sakura) we did the same thing.4- come Monday morning the whole set was prepared and everything started again. Now, before I retired all the method was changed and this part probably you will not attempt: we dewaxed with dishwasher soap (elimination of xylene and ethanol). The slides went directly to the hematoxylin ? bluing ? differentiation ? water ? eosin ? wash water and from then directly to an oven at 60?C for 15 minutes (elimination of ethanol and xylene)?? coverslip So, try as I describe in points 1 to 4 or you can even could try eliminating xylene and ethanol and "go green" and "dry".Ren? On Monday, July 13, 2015 1:30 PM, "Hannen, Valerie" wrote: #yiv6464311563 #yiv6464311563 -- _filtered #yiv6464311563 {font-family:Helvetica;panose-1:2 11 6 4 2 2 2 2 2 4;} _filtered #yiv6464311563 {font-family:Helvetica;panose-1:2 11 6 4 2 2 2 2 2 4;} _filtered #yiv6464311563 {font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;} _filtered #yiv6464311563 {font-family:Tahoma;panose-1:2 11 6 4 3 5 4 4 2 4;}#yiv6464311563 #yiv6464311563 p.yiv6464311563MsoNormal, #yiv6464311563 li.yiv6464311563MsoNormal, #yiv6464311563 div.yiv6464311563MsoNormal {margin:0in;margin-bottom:.0001pt;font-size:12.0pt;}#yiv6464311563 a:link, #yiv6464311563 span.yiv6464311563MsoHyperlink {color:blue;text-decoration:underline;}#yiv6464311563 a:visited, #yiv6464311563 span.yiv6464311563MsoHyperlinkFollowed {color:purple;text-decoration:underline;}#yiv6464311563 span.yiv6464311563EmailStyle17 {color:#1F497D;}#yiv6464311563 .yiv6464311563MsoChpDefault {font-size:10.0pt;} _filtered #yiv6464311563 {margin:1.0in 1.0in 1.0in 1.0in;}#yiv6464311563 div.yiv6464311563WordSection1 {}#yiv6464311563 Rene, ?As I stated before and maybe I was not entirely clear, we had this problem ( little Eosin in the sections of the first rack of slides on Monday morning) before, but had remedied it by starting to change the Eosin on Monday morning before the first rack of slides was run.? He now is stating that the problem of little Eosin in the sections from the first rack on Monday A.M. is starting again.? Since the last fix of the problem we have not deviated in any way, procedure or chemical wise.? We have used the same lot # in previous weeks for the Eosin as we currently have on the stainer ( I changed the Eosin this morning) , so I don?t think it is an Eosin problem per se. ?Kari, ?We do use 95% alcohol before our Eosin at all times.? I would think that if the alcohol before the ?Eosin was the problem, all of my racks would have the ?little Eosin in the sections? problem.. not just the first rack. ?Again any and all suggestions are welcomed!! ?Thanks again Rene and Kari for replying. ?Valerie ?From: Rene J Buesa [mailto:rjbuesa at yahoo.com] Sent: Monday, July 13, 2015 11:20 AM To: Hannen, Valerie; Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Eosin ?It is difficult to advise you anything without knowing what is your PT's complaint. What he does complain about? Ren? ? ?On Monday, July 13, 2015 11:17 AM, "Hannen, Valerie" wrote: ?Good morning, I once again am dealing with a my picky Pathologist!!? About a year ago, he started to complain about not having enough Eosin on his sections( first morning rack on Monday).. I went from changing the Eosin on Friday and stirring it on Monday to totally changing it? on Monday...it has all been good until the end of last week. The problem has started up again... little Eosin in the first rack of Monday morning.? Any suggestions?? Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================"This email is intended solely for the use of the individual towhom it is addressed and may contain information that isprivileged, confidential or otherwise exempt from disclosureunder applicable law. If the reader of this email is not theintended recipient or the employee or agent responsible fordelivering the message to the intended recipient, you arehereby notified that any dissemination, distribution, orcopying of this communication is strictly prohibited. If youhave received this communication in error, please immediatelydelete this message. Thank you"?====================================== From Nancy.Stedman at buschgardens.com Mon Jul 13 14:12:21 2015 From: Nancy.Stedman at buschgardens.com (Stedman, Nancy) Date: Mon, 13 Jul 2015 19:12:21 +0000 Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories In-Reply-To: <04EE4F75BB5FB246ADB68D69B7460443A37D48807E@MAIL.nrhnt.nrh-ok.com> References: <04EE4F75BB5FB246ADB68D69B7460443A37D48807E@MAIL.nrhnt.nrh-ok.com> Message-ID: <18D791D4EE07BC41BF05F8EF3CCCDE2467973A87@FTCSEAP4001.nam.int.local> We are using Excell Plus made by American Master Tech. It is a combination of alcohol, ethylene glycol, and glyoxal. It does not fix quite as fast as formalin but fixes just as well as long as tissue samples are small and not too fatty. We work in veterinary pathology and mostly with necropsy tissues, and try to keep everything 5 to 10 mm maximum thickness that goes into Excell Plus. It definitely won't fix an entire intact brain no matter how long you let it sit so we book the brains into thin slices 24 hours after collection. We have done some limited IHC and DNA extraction from Excell Plus fixed tissue and have not had any issues. We tried another low tox fixative too - Statlab's GTF formalin substitute, which is glyoxal - and it did not fix as well as Excell Plus. GTF fixed CNS tissues especially had a lot of artifact like shrunken neurons. Hope this is helpful. I would also be curious to know how many labs use formalin substitutes because it seems like they have not caught on much (or at all) in the veterinary world. -Nancy Nancy L. Stedman DVM PhD Dipl ACVP Veterinary Pathologist Busch Gardens Tampa nancy.stedman at buschgardens.com -----Original Message----- From: Adesupo, Adesuyi (Banjo) [mailto:abadesuyi at nrh-ok.com] Sent: Monday, July 13, 2015 12:52 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories Hi, I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning. I wanted to know whether some of you guys out there are using Formaldehyde substitute. Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 abadesuyi at nrh-ok.com ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper at chla.usc.edu Mon Jul 13 14:50:40 2015 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Mon, 13 Jul 2015 19:50:40 +0000 Subject: [Histonet] CMV antibody clone Message-ID: Good afternoon Histonet, Just taking a quick poll to find out which CMV clone most folks are running in their labs. Your feedback is much appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From Richard.Cartun at hhchealth.org Mon Jul 13 15:07:50 2015 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 13 Jul 2015 20:07:50 +0000 Subject: [Histonet] CMV antibody clone In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E654C8E6A@HHCEXCHMB03.hhcsystem.org> We use DAKO's (an Agilent Technologies Company) product number M0854 (clones DDG9 & CCH2). Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Cooper, Brian [mailto:bcooper at chla.usc.edu] Sent: Monday, July 13, 2015 3:51 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CMV antibody clone Good afternoon Histonet, Just taking a quick poll to find out which CMV clone most folks are running in their labs. Your feedback is much appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Joyce.Weems at emoryhealthcare.org Mon Jul 13 15:09:44 2015 From: Joyce.Weems at emoryhealthcare.org (Weems, Joyce K.) Date: Mon, 13 Jul 2015 20:09:44 +0000 Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories In-Reply-To: <18D791D4EE07BC41BF05F8EF3CCCDE2467973A87@FTCSEAP4001.nam.int.local> References: <04EE4F75BB5FB246ADB68D69B7460443A37D48807E@MAIL.nrhnt.nrh-ok.com> <18D791D4EE07BC41BF05F8EF3CCCDE2467973A87@FTCSEAP4001.nam.int.local> Message-ID: The problem in the clinical world is that many clinical trials require formalin fixation, so I would not want to substitute anything that would prevent a patient from being in a trial. It's not new to me that formalin is a carcinogen. I just try to make sure to use PPE and have good ventilation and monitor as necessary. (Unlike when I put my ungloved hands in it and had no ventilation when I started down this path a hundred years ago.) j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Stedman, Nancy [mailto:Nancy.Stedman at buschgardens.com] Sent: Monday, July 13, 2015 3:12 PM To: Adesupo, Adesuyi (Banjo); 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories We are using Excell Plus made by American Master Tech. It is a combination of alcohol, ethylene glycol, and glyoxal. It does not fix quite as fast as formalin but fixes just as well as long as tissue samples are small and not too fatty. We work in veterinary pathology and mostly with necropsy tissues, and try to keep everything 5 to 10 mm maximum thickness that goes into Excell Plus. It definitely won't fix an entire intact brain no matter how long you let it sit so we book the brains into thin slices 24 hours after collection. We have done some limited IHC and DNA extraction from Excell Plus fixed tissue and have not had any issues. We tried another low tox fixative too - Statlab's GTF formalin substitute, which is glyoxal - and it did not fix as well as Excell Plus. GTF fixed CNS tissues especially had a lot of artifact like shrunken neurons. Hope this is helpful. I would also be curious to know how many labs use formalin substitutes because it seems like they have not caught on much (or at all) in the veterinary world. -Nancy Nancy L. Stedman DVM PhD Dipl ACVP Veterinary Pathologist Busch Gardens Tampa nancy.stedman at buschgardens.com -----Original Message----- From: Adesupo, Adesuyi (Banjo) [mailto:abadesuyi at nrh-ok.com] Sent: Monday, July 13, 2015 12:52 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories Hi, I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning. I wanted to know whether some of you guys out there are using Formaldehyde substitute. Best regards, Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 abadesuyi at nrh-ok.com ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From paula at excaliburpathology.com Mon Jul 13 15:42:22 2015 From: paula at excaliburpathology.com (Paula Pierce) Date: Mon, 13 Jul 2015 20:42:22 +0000 (UTC) Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories In-Reply-To: References: Message-ID: <141916136.1652861.1436820142833.JavaMail.yahoo@mail.yahoo.com> Like and Ditto to Joyce.?Paula Pierce, BS, HTL(ASCP)HT?President?Excalibur Pathology, Inc.?5830 N Blue Lake Dr.?Norman, OK 73069?405-759-3953 PH??405-759-7513 FAX?www.excaliburpathology.com From: "Weems, Joyce K." To: "Stedman, Nancy" ; "Adesupo, Adesuyi (Banjo)" ; "'histonet at lists.utsouthwestern.edu'" Sent: Monday, July 13, 2015 3:09 PM Subject: Re: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories The problem in the clinical world is that many clinical trials require formalin fixation, so I would not want to substitute anything that would prevent a patient from being in a trial. It's not new to me that formalin is a carcinogen. I just try to make sure to use PPE and have good ventilation and monitor as necessary. (Unlike when I put my ungloved hands in it and had no ventilation when I started down this path a hundred years ago.)? j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems at emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s).? It may contain information that is privileged and confidential.? Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Stedman, Nancy [mailto:Nancy.Stedman at buschgardens.com] Sent: Monday, July 13, 2015 3:12 PM To: Adesupo, Adesuyi (Banjo); 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories We are using Excell Plus made by American Master Tech.? It is a combination of alcohol, ethylene glycol, and glyoxal.? It does not fix quite as fast as formalin but fixes just as well as long as tissue samples are small and not too fatty.? We work in veterinary pathology and mostly with necropsy tissues, and try to keep everything 5 to 10 mm maximum thickness that goes into Excell Plus.? It definitely won't fix an entire intact brain no matter how long you let it sit so we book the brains into thin slices 24 hours after collection.? We have done some limited IHC and DNA extraction from Excell Plus fixed tissue and have not had any issues.? We tried another low tox fixative too - Statlab's GTF formalin substitute, which is glyoxal - and it did not fix as well as Excell Plus.? GTF fixed CNS tissues especially had a lot of artifact like shrunken neurons. Hope this is helpful.? I would also be curious to know how many labs use formalin substitutes because it seems like they have not caught on much (or at all) in the veterinary world. -Nancy Nancy L. Stedman DVM PhD Dipl ACVP Veterinary Pathologist Busch Gardens Tampa nancy.stedman at buschgardens.com -----Original Message----- From: Adesupo, Adesuyi (Banjo) [mailto:abadesuyi at nrh-ok.com] Sent: Monday, July 13, 2015 12:52 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories ? ? Hi, ? ? ? I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning. ? ? I wanted to know whether some of you guys out there are using Formaldehyde substitute. ? ? Best regards, ? Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS ? Histology Supervisor ? Norman Regional Health System, ? Norman, OK 73071. ? Tel: 405- 307- 1145 ? abadesuyi at nrh-ok.com ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jvickroy at SpringfieldClinic.com Mon Jul 13 16:27:39 2015 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Mon, 13 Jul 2015 21:27:39 +0000 Subject: [Histonet] Advice needed on doing the technical component and professional component at two different sites Message-ID: <9B1A1501A800064397369BD8072E6BCA06425831@E2K10DB.springfieldclinic.com> Currently our dermatologists send their skin biopsies to a private lab. Since we now have our own Histology department we are proposing to do the technical component (grossing and preparation of slides) in house and sending the block and H&E slides to the same private company. They call these type of clients " professional read only". I understand the differences regarding billing since the CPT codes we are using have a professional component and a technical component. My questions center around what other organizations do for accessioning and how they meet CAP requirements for grossing, etc. Normally we handle all of our other specimens in the normal way of accessioning, grossing (under the indirect supervision of a pathologist that comes to read the slides, processing, preparation of slides, and giving the slides to the pathologist who signs out the surgical report including the gross, and the rest of the surgical report. The dermatology specimens would have to be handled differently. Our dermatologists want the slides read by the private company that only does dermatology specimens. (at least until we hire a dermatopathologist). In the past the derm tissues were sent to the private company and they did all of the process including grossing. Our organization has now decided that it is advantageous for us to do the technical portion in house and then send the slides to be read by the private company because of changes in billing. The private company is perfectly willing to help us make sure they get information they need to diagnose the cases (they have even shown us how they like each type of skin biopsy sectioned and inked. They will help us set up a system for them to get the slides and do the surgical reports. Their report will have our gross description and surgical number, along with a statement that the gross and processing was done elsewhere. They have done this with several other clients. I have some unanswered questions since I have never done something like this. Below is a list of my questions. What I really need is someone to tell me how they are handling specimens that they prepare locally and then send the slides to a pathology group to read. 1. Since the local pathologist group does not read these slides, are our grossing techs under the indirect supervision of the pathologists at the private company for these specimens? 2. How do you accession these specimens locally so tracking, slide preparation, and technical component billing can be done, without a local surgical report. 3. If you have to do a local report, who signs the gross report that just has a gross and maybe a statement that the slides will be read at the private company? 4. Are there other CAP considerations I am not thinking of? Am I making this too complicated? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From owensc500 at gmail.com Mon Jul 13 18:22:35 2015 From: owensc500 at gmail.com (Clarence Owens) Date: Mon, 13 Jul 2015 19:22:35 -0400 Subject: [Histonet] HPV Message-ID: <8782DE67-B4F6-40B4-8757-955297B578E1@gmail.com> Hello Histonetters: Maybe one of you could lend a hand. I am currently working on FISH validation and I am in the need of HPV positive tissue of the following subtypes: 18, 31, 33, and 51. Does anyone have any extra positive HPV tissue they can spare expressing the above subtypes? I thank you for your help. Regards, Clarence Owens, HT (ASCP)cm, QIHC Sent from my iPhone From bcooper at chla.usc.edu Mon Jul 13 19:34:13 2015 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Tue, 14 Jul 2015 00:34:13 +0000 Subject: [Histonet] CMV antibody clone In-Reply-To: References: Message-ID: Thank you to everyone who replied to this post. In case you're wondering, I got like 8 responses today, and everyone who responded uses CMV (DDG9/CCH2) from either Cell Marque or Dako. You guys are the best! Thanks, Brian -----Original Message----- From: Cooper, Brian [mailto:bcooper at chla.usc.edu] Sent: Monday, July 13, 2015 12:51 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CMV antibody clone Good afternoon Histonet, Just taking a quick poll to find out which CMV clone most folks are running in their labs. Your feedback is much appreciated. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From jkiernan at uwo.ca Tue Jul 14 00:11:25 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Tue, 14 Jul 2015 00:11:25 -0500 Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories In-Reply-To: <711084563c459.55a499dc@uwo.ca> References: <04EE4F75BB5FB246ADB68D69B7460443A37D48807E@MAIL.nrhnt.nrh-ok.com> <728086ad3fd72.55a48691@uwo.ca> <71e09e673dea9.55a48709@uwo.ca> <71e0b11f3ece4.55a48748@uwo.ca> <73e0ea6a3aa36.55a48785@uwo.ca> <7270dae93ee22.55a487c4@uwo.ca> <7270f9f53ca1e.55a48802@uwo.ca> <727088a93884b.55a48840@uwo.ca> <7370ae543b534.55a4887e@uwo.ca> <71e093753e0c4.55a488bc@uwo.ca> <71e0fba7399df.55a488fa@uwo.ca> <7100bcaf3ad18.55a48938@uwo.ca> <7100c6de3ec75.55a489b2@uwo.ca> <7100b0a33e12b.55a489f0@uwo.ca> <711098e63903f.55a48a2e@uwo.ca> <73e0b1193f8e5.55a48a6d@uwo.ca> <7350f9733dabd.55a48aab@uwo.ca> <7340ecba3b865.55a48aea@uwo.ca> <7340c50d3b73a.55a48b28@uwo.ca> <7340ff7d3b20d.55a48b68@uwo.ca> <7340f8a439866.55a48b88@uwo.ca> <7340c6153d93b.55a48bc6@uwo.ca> <7280ea18385a7.55a48c05@uwo.ca> <7100cfbe3887d.55a48c43@uwo.ca> <7100b4d83d5e5.55a48c82@uwo.ca> <7330c6293b1ce.55a48cc0@uwo.ca> <733089f839fc4.55a48d3b@uwo.ca> <7280fe0b39d37.55a48d79@uwo.ca> <7280b6543e377.55a48db8@uwo.ca> <7370dc753b728.55a48df6@uwo.ca> <7360fbbb3a4d8.55a48e34@uwo.ca> <71e097003b50b.55a48e73@uwo.ca> <7340e1703a97d.55a48eb1@uwo.ca> <7330ebf73ffb8.55a48ef1@uwo.ca> <7370e96539701.55a48f2f@uwo.ca> <7110ea583dc95.55a48f6e@uwo.ca> <7110ea5c3bb2b.55a48fad@uwo.ca> <7110f0083b8c4.55a48feb@uwo.ca> <71e0e6843e305.55a4902a@uwo.ca> <71e0e04539106.55a49068@uwo.ca> <71e0a1023a817.55a490a8@uwo.ca> <7360cb6c3933b.55a490e7@uwo.ca> <7360bbd43967e.55a49125@uwo.ca> <7360d7563ea1e.55a49164@uwo.ca> <73e0aceb3bbcd.55a492d0@uwo.ca> <73e0d4c43c865.55a4930f@uwo.ca> <73e0d39b3e548.55a4934e@uwo.ca> <733088b73cb58.55a4938c@uwo.ca> <7330f82b38e35.55a493cb@uwo.ca> <737097713c805.55a49409@uwo.ca> <73708f603bb94.55a49448@uwo.ca> <73e0b4833c092.55a4953a@uwo.ca> <71e0d058396b9.55a49579@uwo.ca> <7100bf383af30.55a495b8@uwo.ca> <710086a739e41.55a495fa@uwo.ca> <7100dc083ce1e.55a49637@uwo.ca> <7100a7c03ce55.55a496b2@uwo.ca> <7100e72e38a35.55a496f1@uwo.ca> <7370b2ea39764.55a49730@uwo.ca> <735095fb3b95c.55a49770@uwo.ca> <735084283913f.55a49827@uwo.ca> <735097da39cc1.55a49866@uwo.ca> <7370d3cb3fc36.55a498a4@uwo.ca> <73308eda3a1a0.55a498e3@uwo.ca> <733095913c2bf.55a49921@uwo.ca> <73309c8f3822f.55a49960@uwo.ca> <7110994c3f3ce.55a4999e@uwo.ca> <711084563c459.55a499dc@uwo.ca> Message-ID: <7110fe8b3a9d8.55a453ad@uwo.ca> Dear Banjo, A reference to the article would be helpful; there must be more to it than one sentence! Formaldehyde has been known for decades to be hazardous, and there are safety regulations in places where it is used. Plenty of old-timers are still alive and well after woking with formaldehyde in the days when there were few or no regulations. I'm one of them. ? >From about 1895 until about 1995 (and perhaps still, in some universities), every medical student spent most of the working day for at least a year with his or her nose and bare hands in a cadaver that had been embalmed in a cocktail containing phenol and formaldehyde. The predominant smell was the phenol, except when dissecting brains, which were fixed and stored in 4% formaldehyde. About 35 years ago, the American Association of Anatomists investigated effects of exposure to embalming chemicals on teachers of anatomy, who are in the dissecting room year after year. The only significant finding was eczema on the hands of some people, long known to be avoidable by wearing rubber gloves. Yes, I too should be able to provide a reference, but this was in the days of paper, which gets thrown out to make room for more paper ... There might be something deep in the archives at http://www.anatomy.org/ Other chemicals used in anatomy, pathology and histology labs also have their dangers; we avoid drinking them, rubbing them into our skin and inhaling their vapours, and we do our best to observe the safety regulations when it comes to getting rid of them. There is no "substitute" fixative functionally identical to formaldehyde. There are other fixatives, some less hazardous, but they have different effects on staining properties etc. The late Holde Puchtler published papers urging pathologists to use non-aqueous coagulant fixatives for routine fixation of small specimens, with her Carnoy variant "methacarn" (methanol 60, acetic acid 10, chloroform 30) as the probable best, also good for some modern molecular methods. For this I can provide a few references: Puchtler, H., Waldrop, F.S., Meloan, S.N., Terry, M.S. and Connor, H.M. (1970). Methacarn (methanol-Carnoy) fixation. Practical and theoretical considerations. Histochemie 21:97-116. Cox, M.L., Schray, C.L., Luster, C.N., Stewart, Z.S., Korytko, P.J., Khan, K.N.M., Paulauskis, J.D. and Dunstan, R.W. (2006). Assessment of fixatives, fixation and tissue processing on morphology and RNA integrity. Experimental and Molecular Pathology 80:183-191. Buesa, R.J. (2008). Histology without formalin? Annals of Diagnostic Pathology 12:387-396. Uneyama, C., Shibutani, M., Masutomi, N., Takagi, H. and Hirose, M. (2002). Methacarn fixation for genomic DNA analysis in microdissected paraffin-embedded tissue specimens. Journal of Histochemistry and Cytochemistry 50:1237-1245. Milcheva, R., Janega, P., Celec, P., Russev, R. and Babal, P. (2013). Alcohol based fixatives provide excellent tissue morphology, protein immunoreactivity and RNA integrity in paraffin embedded tissue specimens. Brain Research Protocols 115:279-289. Greer, C.E., Peterson, S.L., Kiviat, N.B. and Manos, M.M. (1991). PCR amplification from paraffin-embedded tissues. American Journal of Clinical Pathology 95:117-124. Tissue processing is extremely simple after non-aqueous coagulant fixation, and most of the stages of a processing machine are not needed. Nuclear chromatin details are much sharper than after formaldehyde. This may not be seen as a blessing by young and middle-aged pathologists. In bygone days the routine fixatives contained mercuric chloride, which gives crisp chromatin and cytoplasmic details. The heterochromatin details probably are artifacts of fixation, but they are useful for identifying cells. John Kiernan Old neuroanatomist and histochemist UWO, London, Canada http://publish.uwo.ca/~jkiernan/ Also Secretary, Biological Stain Commission http://biostain.com = = = On 13/07/15, "Adesupo, Adesuyi (Banjo)" wrote: > Hi, > I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning. > I wanted to know whether some of you guys out there are using Formaldehyde substitute. > > > Best regards, > > Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS > Histology Supervisor > Norman Regional Health System, > Norman, OK 73071. > Tel: 405- 307- 1145 > abadesuyi at nrh-ok.com> > > ====================================== > CONFIDENTIALITY NOTICE: > > This e-mail communication and any attachments may > contain confidential and privileged information for the use > of the designated recipients named above. If you are not > the intended recipient, you are hereby notified that you > have received this communication in error and that any > review, disclosure, dissemination, distribution, or copying > of it or its contents is prohibited. If you have received > this communication in error, please notify the sender > immediately and destroy all copies of this communication > and any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From lmdee1 at yahoo.com Tue Jul 14 04:26:06 2015 From: lmdee1 at yahoo.com (Linda) Date: Tue, 14 Jul 2015 09:26:06 +0000 (UTC) Subject: [Histonet] Advice needed on doing the technical component and professional component at two different sites In-Reply-To: <9B1A1501A800064397369BD8072E6BCA06425831@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA06425831@E2K10DB.springfieldclinic.com> Message-ID: <1122860962.1781315.1436865966801.JavaMail.yahoo@mail.yahoo.com> Jim, I know how you feel with confusing about not having a pathologist onsite.? I have lived your next adventure for?over 5 years.? This is what I have done- 1.? ? ? ? Since the local pathologist group does not read these slides,? are our? grossing techs under the indirect supervision of the pathologists at the private company for these specimens?? ?No, you are not under indirect supervision. You are considered a reference lab.? Whom ever is your lab director is your supervising person.? But, if the director doesn't review any of same work batch, then you will need QC documentation from the private company.? And, your lab director will have to sign off after reviewing private lab documents. 2? How do you accession these specimens locally so tracking, slide preparation, and technical component billing can be done, without a local surgical report.? ?You are going to have to document all high complexity testing.? I have a tracking sheet that lists- accession #, patient's name, block#, general tissue type, grossing #, embed #, # of H&E's sent, specials sent, who checked in? received?slides and (we receive back a slide?level for our records) who sent back the slide.? I have a separate histology req, which is dictated straight into the patient's chart using dragon and a grossing template which lists our TC charges.? I have no LIS system.? The final report is interfaced using HL7 windows. 3.?? If you have to do a local report, who signs the gross report that just has a gross and maybe a statement that the slides will be read at the private company? I electronically sign my gross report.?Yes, I do meet the CLIA and CAP requirements for doing high complexity testing. ?My grossing template includes my CLIA and CAP number and a statement mentioning?all technical component was performed at?X company with address,?phone, etc.? 4.? ? ? Are there other CAP considerations I am not thinking of?? I do not know what policies and procedures you currently have in place, so this question is harder to answer.? BUT you do have to have documentation of all shipping and tracking numbers to show CAP inspectors and in case something goes awry in passage of slides. A very important point to remember is BOTH sets of charges have to match or you will be red flagged by insurance companies.? If the reading pathologist doesn't use the special stain, you can not charge. You have to have a copy of whom ever is reading your slides credentials and insurance, continuing ed,etc. You have to have ready for inspectors- person doing grossing credentials and documentation. CAP is very good with answering questions.? You do have to comb through all the CAP regulations to see what applies and what does not. If you are not already CAP accredited, they have a team(which you pay) who can check to see if you are ready for accreditation.? Good Luck, Linda Dee, BGS, HT(ASCP) ? From: "Vickroy, James" To: "histonet at lists.utsouthwestern.edu" Sent: Monday, July 13, 2015 4:27 PM Subject: [Histonet] Advice needed on doing the technical component and professional component at two different sites Currently our dermatologists send their skin biopsies to a private lab.? Since we? now have our own Histology department we are proposing to do the technical component (grossing and preparation of slides) in house and sending the block and H&E slides to the same private company.? They call these type of clients " professional read only".? I understand the differences regarding billing since the CPT codes we are using have a professional component and a technical component.? My questions center around what other organizations do for accessioning and how they? meet CAP requirements for grossing, etc. Normally we handle all of our other specimens in the normal way of accessioning, grossing (under the indirect supervision of a pathologist that comes to read the slides, processing, preparation of slides, and giving the slides to the pathologist who signs out the surgical report including the gross, and the rest of the surgical report. The dermatology specimens would have to be handled differently. Our dermatologists want the slides read by the private company that only does dermatology specimens. (at least until we hire a dermatopathologist).? In the past the derm tissues were sent to the private company and they did all of the process including grossing.? Our organization has now decided that it is advantageous for us to do the technical portion in house and then send the slides to be read by the private company because of changes in billing.? The private company is perfectly willing to? help us make sure they get information they need? to diagnose the cases (they have even shown us how they like each type of skin biopsy sectioned and inked.? They will help us set up a system for them to get the slides and do the surgical reports.? Their report will have our gross description and surgical number, along with a statement that the gross and processing was done elsewhere.? They have done this with several other clients.? I have some unanswered questions since I have never done something like this.? Below is a list of my questions.? What I really need is someone to tell me how they are handling specimens that they prepare locally and then send the slides to a pathology group to read. 1.? ? ? ? Since the local pathologist group does not read these slides,? are our? grossing techs under the indirect supervision of the pathologists at the private company for these specimens? 2.? ? ? How do you accession these specimens locally so tracking, slide preparation, and technical component billing can be done, without a local surgical report. 3.? ? ? If you have to do a local report, who signs the gross report that just has a gross and maybe a statement that the slides will be read at the private company? 4.? ? ? Are there other CAP considerations I am not thinking of? Am I making this too complicated? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois? 62703 Office:? 217-528-7541, Ext. 15121 Email:? jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mkent at dermpathlab.com Tue Jul 14 07:40:06 2015 From: mkent at dermpathlab.com (Michael Kent) Date: Tue, 14 Jul 2015 08:40:06 -0400 Subject: [Histonet] competence assessment pathologist Message-ID: Good morning and sincere thanks to all those taking time to respond to our many questions. I am looking for a template/example of a good competence assessment for pathologists, meeting CAP&NY standards. Appreciate any references. Thank you, Mike From JGarrigan at buffalobiolabs.com Tue Jul 14 09:13:40 2015 From: JGarrigan at buffalobiolabs.com (Jennifer Garrigan) Date: Tue, 14 Jul 2015 14:13:40 +0000 Subject: [Histonet] Brady Lapxpert Message-ID: Hi All, We have a Brady LabXpert that does not work and we have replaced it with the BMP 51. The label cartridges are no longer compatible and we have a lot of them in varying conditions. We have one brand new in box, we have several that are opened from the box but still are complete rolls and have the yellow tape over them and some partially used. Is anyone interested in purchasing these? Feel free to make an offer. We would prefer to mail them out as a large batch via fedex, with the recipient paying. Brand new in box X-127-482 Qty 1 New but opened/ out of box (still have yellow tape across the opening) XC-1000-595-GM-WT Qty 4 X-127-482 Qty 4 X-97-488 Qty 2 XC-500-492 Qty 1 X-83-492 Qty 1 X-120-499 Qty 1 Partially used/open would sell at a very large discount or discard X-120-499 Qty 2 (I would say close to 1 full) X-83-492 Qty 1 (I would say half or less) XC-1000-595-GN-WT Qty 2 (Just about full) XC-500-595-WT-BK Qty 1 (less than half) X-103-498 (almost full) X-131-498 Qty 2 (about 1 full together) XC-500-492 Qty 3 (about one and a half full) Thank you very much! Jennifer Garrigan, LVT, AS, BA Senior Research Technician Buffalo BioLabs LLC 73 High Street Buffalo, NY 14203 (716)849-6810 x 357 jgarrigan at buffalobiolabs.com From idimitro at mun.ca Tue Jul 14 09:25:33 2015 From: idimitro at mun.ca (idimitro at mun.ca) Date: Tue, 14 Jul 2015 14:25:33 +0000 Subject: [Histonet] Decalcification of mouse jaw and legs In-Reply-To: <959202AC61AEF942968646EC66E2BE3EACEA5CF0@ESPWMSGMBX08.CSMC.EDU> References: <959202AC61AEF942968646EC66E2BE3EACEA5CF0@ESPWMSGMBX08.CSMC.EDU> Message-ID: <14C3108E8B98EF43B3EDAE583367003401C87FE837@exchange.med.mun.ca> We do mouse legs routinely, either with Cal Ex II or EDTA. We look at the bone and the tissue around, so we have to have good fixation, decal., cutting and staining for both. Cal Ex II (formic acid) is a gentler decal agent than HCL (Plain "Cal EX") - and Cal Ex II keeps the H&E staining true - whereas HCL would make the Eosin dominate and stain everything! For future - since Cal Ex II contains Formalin - on theory you can fix and decal at the same time, but I always do my fixation first and then decal after. Use chemical method to determine the end point. Other ways to decal bone are with EDTA - I personally prefer it, though it takes a really long time, almost 2 months, it is better if you want to do IHC for certain markers that the acids might interfere with. We weight the samples before we start decal, and after that 2/week. The moment you stop seeing decrease in weight and see slight increase, that is the end point. For cutting, I prefer samples decalc. with Cal Ex II, cuts without a problem. Iliana Dimitrova, MLT, LHP, B.Tech., M.Sc. Histology Supervisor Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University of Newfoundland St. John's, NL Canada A1B 3V6 This email and its contents may contain confidential and/or private information and is intended for the sole use of the addressee(s). If you are not the named addressee you should not disseminate, distribute or copy this email. If you believe that you received this email in error please notify the original sender and immediately delete this email and all attachments. Except where properly supported with required and authorized documents, no legal or financial obligation will be incurred by Memorial University as a result of this communication. -----Original Message----- From: Swartwood, Steven J [mailto:Steven.Swartwood at cshs.org] Sent: July-10-15 7:23 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Decalcification of mouse jaw and legs Hello all, I hope everyone is having or had a fantastic week, I'm going to test a few different decal methods on mouse jaws and leg bones. I was wondering if anyone does this routinely out there who is willing to share a protocol. I've never done this on mouse jaw/leg bones before. I've only done this on human tissues. From what I've read it seems that for routine histology low % HCl is fine, but if I wanted to perform IHC then anywhere from 5-15% formic acid may be the best way to go. Nitric acid is probably too strong of a decal agent for these tiny specimens I would assume. Maybe an EDTA based decal agent may be best as well. I'm just really inexperienced with this and I'm very open to ideas and trying a few different methods. Steven Swartwood HT(ASCP) IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is strictly prohibited. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tfountain at dsmanitoba.ca Tue Jul 14 09:42:02 2015 From: tfountain at dsmanitoba.ca (Tiana Baskin) Date: Tue, 14 Jul 2015 14:42:02 +0000 Subject: [Histonet] DAKO LINK PTs Message-ID: Hi Histonetters, I have encountered a problem with some of my staining and I am struggling to pinpoint the root cause. I was wondering if any other DAKO Autostainer Link/PT users are experiencing the same oddities. It seems like the first runs with fresh HIER PT solution is very typical of what we have optimized and the second batch of slides has more background and in some cases nuclear staining (especially in Actin (1A4) and pan CK (AE1/AE3)) when the target is cytoplasmic. We do not use the PT solution more than twice. What do others do? Have you seen similar things? Tiana -------------- next part -------------- This email and/or any documents in this transmission is intended for the addressee(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. Ce courriel et tout document dans cette transmission est destin? ? la personne ou aux personnes ? qui il est adress?. Il peut contenir des informations privil?gi?es ou confidentielles. Toute utilisation, divulgation, distribution, copie, ou diffusion non autoris?e est strictement d?fendue. Si vous n'?tes pas le destinataire de ce message, veuillez en informer l'exp?diteur imm?diatement et lui remettre l'original. From liz at premierlab.com Tue Jul 14 11:13:16 2015 From: liz at premierlab.com (Elizabeth Chlipala) Date: Tue, 14 Jul 2015 10:13:16 -0600 Subject: [Histonet] DAKO LINK PTs In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE02230F9D9029@SBS2K8.premierlab.local> Tiana We have never experienced any issue like that here we will use the HIER solutions in the PT links for up to three times within a period of 2 weeks. We have had our PT link units validated and they are calibrated yearly. Do you review the Target Retrieval Run Reports, we print and keep all of ours. Our units are primarily programed to heat to 80C we put the slides in, warm up to 95C retrieve for 20 minutes cool down to 80C remove slides right after that. I would think if you left slides in the retrieval solution for different times after they are completed you might see some changes in staining intensity, that why we try to be consistent and remove the slides as soon as they are completed. We do use other retrieval times and temps on some occasions but what I stated is our standard protocol. I hope this helps. There is one other thing, it think it is extremely important to clean the instrument as it is required, we have in our SOP's after 200 slides I don't know what Dako recommends but we actually clean more often typically after around 80 to 120 slides or so. That will keep your probe nice and clean and decrease your variability, if you don't clean you can get build up in the probe and that is just going to cause inconsistent staining, its easy to set up a cleaning cycle at the end of the day. There are also a lot of other factors that can affect staining consistency - tissue placement on the slide in one thing, we place our tissue in the same area on the slide, same number of drop zones, appropriate amount of reagents, cutting corners on amount of reagents will not lead to consistent high quality staining. Section thickness can also lead to variation in staining intensity. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: Tiana Baskin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, July 14, 2015 8:42 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] DAKO LINK PTs Hi Histonetters, I have encountered a problem with some of my staining and I am struggling to pinpoint the root cause. I was wondering if any other DAKO Autostainer Link/PT users are experiencing the same oddities. It seems like the first runs with fresh HIER PT solution is very typical of what we have optimized and the second batch of slides has more background and in some cases nuclear staining (especially in Actin (1A4) and pan CK (AE1/AE3)) when the target is cytoplasmic. We do not use the PT solution more than twice. What do others do? Have you seen similar things? Tiana From FMonson at wcupa.edu Tue Jul 14 11:59:53 2015 From: FMonson at wcupa.edu (Monson, Frederick) Date: Tue, 14 Jul 2015 16:59:53 +0000 Subject: [Histonet] Eosin In-Reply-To: <41400FFE517878449D89114DD2526090196602D1BB@tocmail1.tocad.orclinic.com> References: <450B7A81EDA0C54E97C53D60F00776C32337384270@isexstore03> <41400FFE517878449D89114DD2526090196602D1BB@tocmail1.tocad.orclinic.com> Message-ID: <4ded7b6fdff4484a8c3e332562791c88@WCUXCHP08.PASSHE.LCL> Long ago, in the foggy, foggy past, we were admonished to insure that our Eosin in the bottle had a slight 'green' tinge in good daylight. When we finally got fluorescence, we would check an H&E-stained section with the fluorescein filters to insure the fluorescence of the Eosin. Very scientific, I know, but when the 'green' was gone, the Eosin was replaced. Cheers, Fred Monson Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pennsylvania Schmucker Science South - Room SSS-024 MailDrop: Geology-Astronomy 750 South Church Street West Chester, PA, 19383 610-738-0437 fmonson at wcupa.edu -----Original Message----- From: Kienitz, Kari [mailto:kkienitz at orclinic.com] Sent: Monday, July 13, 2015 11:21 AM To: Hannen, Valerie; Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Eosin It's always kind of a stab in the dark to diagnose H&E problems without a little more information. One thing I would try if you don't feel its an actual Eosin problem is your alcohol prior to the eosin. Slide volume and humidity can dilute your alcohol and cause lighter cytoplasmic staining. The alcohol prior to eosin should be 95% and changed daily if need be. Kari Kienitz HT, (ASCP) Histology Laboratory Gastroenterology-EAST The Oregon Clinic 1111 NE 99th Ave Portland, OR 97220 503.935.8311 kkienitz at orclinic.com CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive and confidential use of the intended recipient. If you are not the intended recipient, please do not read, distribute or take action in reliance upon this missive. If you have received this in error, please notify the sender immediately by reply e-mail and delete this message and its attachments from your computer system. Thank you ________________________________________ From: Hannen, Valerie [Valerie.Hannen at parrishmed.com] Sent: Monday, July 13, 2015 8:05 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Eosin Good morning, I once again am dealing with a my picky Pathologist!! About a year ago, he started to complain about not having enough Eosin on his sections( first morning rack on Monday).. I went from changing the Eosin on Friday and stirring it on Monday to totally changing it on Monday...it has all been good until the end of last week. The problem has started up again... little Eosin in the first rack of Monday morning. Any suggestions?? Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From af46 at buffalo.edu Tue Jul 14 12:12:19 2015 From: af46 at buffalo.edu (Featherstone, Annette) Date: Tue, 14 Jul 2015 17:12:19 +0000 Subject: [Histonet] Digital Imaging Analyst Position at the University of Buffalo Message-ID: Digital Imaging Analyst Position at The University of Buffalo: Quicklink for Posting: https://www.ubjobs.buffalo.edu/applicants/Central?quickFind+58162 From FMonson at wcupa.edu Tue Jul 14 12:50:04 2015 From: FMonson at wcupa.edu (Monson, Frederick) Date: Tue, 14 Jul 2015 17:50:04 +0000 Subject: [Histonet] Digital Imaging Analyst Position at the University of Buffalo In-Reply-To: References: Message-ID: <14c47596f90a4637b5dff4e3a01e44a6@WCUXCHP08.PASSHE.LCL> A strongly recommended first interview question: "How many digital cameras do you have with you today?" Cheers, FCM Frederick C. Monson, PhD Technical Director Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pennsylvania Schmucker Science South - Room SSS-024 MailDrop: Geology-Astronomy 750 South Church Street West Chester, PA, 19383 610-738-0437 fmonson at wcupa.edu -----Original Message----- From: Featherstone, Annette via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, July 14, 2015 1:12 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Digital Imaging Analyst Position at the University of Buffalo Digital Imaging Analyst Position at The University of Buffalo: Quicklink for Posting: https://www.ubjobs.buffalo.edu/applicants/Central?quickFind+58162 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff at rwjms.rutgers.edu Tue Jul 14 13:13:34 2015 From: mcauliff at rwjms.rutgers.edu (Geoff) Date: Tue, 14 Jul 2015 14:13:34 -0400 Subject: [Histonet] Digital Imaging Analyst Position at the University of Buffalo In-Reply-To: <14c47596f90a4637b5dff4e3a01e44a6@WCUXCHP08.PASSHE.LCL> References: <14c47596f90a4637b5dff4e3a01e44a6@WCUXCHP08.PASSHE.LCL> Message-ID: <55A5514E.4040307@umdnj.edu> All of my cameras are operated digitally. Geoff On 7/14/2015 1:50 PM, Monson, Frederick via Histonet wrote: > A strongly recommended first interview question: "How many digital cameras do you have with you today?" > > Cheers, > > FCM > > Frederick C. Monson, PhD > Technical Director > Center for Microanalysis and Imaging, Research and Training (CMIRT) > West Chester University of Pennsylvania > Schmucker Science South - Room SSS-024 > MailDrop: Geology-Astronomy > 750 South Church Street > West Chester, PA, 19383 > 610-738-0437 > fmonson at wcupa.edu > > > -----Original Message----- > From: Featherstone, Annette via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, July 14, 2015 1:12 PM > To: 'histonet at lists.utsouthwestern.edu' > Subject: [Histonet] Digital Imaging Analyst Position at the University of Buffalo > > Digital Imaging Analyst Position at The University of Buffalo: > > Quicklink for Posting: https://www.ubjobs.buffalo.edu/applicants/Central?quickFind+58162 > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcauliff at rwjms.rutgers.edu ********************************************** From Tom_Wells at bcit.ca Tue Jul 14 13:45:03 2015 From: Tom_Wells at bcit.ca (Tom Wells) Date: Tue, 14 Jul 2015 18:45:03 +0000 Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories Message-ID: Dear Banjo, A reference to the article would be helpful; there must be more to it than one sentence! Formaldehyde has been known for decades to be hazardous, and there are safety regulations in places where it is used. Plenty of old-timers are still alive and well after woking with formaldehyde in the days when there were few or no regulations. I'm one of them. >From about 1895 until about 1995 (and perhaps still, in some universities), every medical student spent most of the working day for at least a year with his or her nose and bare hands in a cadaver that had been embalmed in a cocktail containing phenol and formaldehyde. The predominant smell was the phenol, except when dissecting brains, which were fixed and stored in 4% formaldehyde. About 35 years ago, the American Association of Anatomists investigated effects of exposure to embalming chemicals on teachers of anatomy, who are in the dissecting room year after year. The only significant finding was eczema on the hands of some people, long known to be avoidable by wearing rubber gloves. Yes, I too should be able to provide a reference, but this was in the days of paper, which gets thrown out to make room for more paper ... There might be something deep in the archives at http://www.anatomy.org/ Other chemicals used in anatomy, pathology and histology labs also have their dangers; we avoid drinking them, rubbing them into our skin and inhaling their vapours, and we do our best to observe the safety regulations when it comes to getting rid of them. There is no "substitute" fixative functionally identical to formaldehyde. There are other fixatives, some less hazardous, but they have different effects on staining properties etc. The late Holde Puchtler published papers urging pathologists to use non-aqueous coagulant fixatives for routine fixation of small specimens, with her Carnoy variant "methacarn" (methanol 60, acetic acid 10, chloroform 30) as the probable best, also good for some modern molecular methods. For this I can provide a few references: Puchtler, H., Waldrop, F.S., Meloan, S.N., Terry, M.S. and Connor, H.M. (1970). Methacarn (methanol-Carnoy) fixation. Practical and theoretical considerations. Histochemie 21:97-116. Cox, M.L., Schray, C.L., Luster, C.N., Stewart, Z.S., Korytko, P.J., Khan, K.N.M., Paulauskis, J.D. and Dunstan, R.W. (2006). Assessment of fixatives, fixation and tissue processing on morphology and RNA integrity. Experimental and Molecular Pathology 80:183-191. Buesa, R.J. (2008). Histology without formalin? Annals of Diagnostic Pathology 12:387-396. Uneyama, C., Shibutani, M., Masutomi, N., Takagi, H. and Hirose, M. (2002). Methacarn fixation for genomic DNA analysis in microdissected paraffin-embedded tissue specimens. Journal of Histochemistry and Cytochemistry 50:1237-1245. Milcheva, R., Janega, P., Celec, P., Russev, R. and Babal, P. (2013). Alcohol based fixatives provide excellent tissue morphology, protein immunoreactivity and RNA integrity in paraffin embedded tissue specimens. Brain Research Protocols 115:279-289. Greer, C.E., Peterson, S.L., Kiviat, N.B. and Manos, M.M. (1991). PCR amplification from paraffin-embedded tissues. American Journal of Clinical Pathology 95:117-124. Tissue processing is extremely simple after non-aqueous coagulant fixation, and most of the stages of a processing machine are not needed. Nuclear chromatin details are much sharper than after formaldehyde. This may not be seen as a blessing by young and middle-aged pathologists. In bygone days the routine fixatives contained mercuric chloride, which gives crisp chromatin and cytoplasmic details. The heterochromatin details probably are artifacts of fixation, but they are useful for identifying cells. John Kiernan Old neuroanatomist and histochemist UWO, London, Canada http://publish.uwo.ca/~jkiernan/ Also Secretary, Biological Stain Commission http://biostain.com = = = On 13/07/15, "Adesupo, Adesuyi (Banjo)" wrote: > Hi, > I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning. > I wanted to know whether some of you guys out there are using Formaldehyde substitute. > > > Best regards, > > Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS > Histology Supervisor > Norman Regional Health System, > Norman, OK 73071. > Tel: 405- 307- 1145 > abadesuyi at nrh-ok.com> > > ====================================== > CONFIDENTIALITY NOTICE: > > This e-mail communication and any attachments may > contain confidential and privileged information for the use > of the designated recipients named above. If you are not > the intended recipient, you are hereby notified that you > have received this communication in error and that any > review, disclosure, dissemination, distribution, or copying > of it or its contents is prohibited. If you have received > this communication in error, please notify the sender > immediately and destroy all copies of this communication > and any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SteveM at mcclainlab.com Tue Jul 14 16:24:37 2015 From: SteveM at mcclainlab.com (Steve McClain) Date: Tue, 14 Jul 2015 21:24:37 +0000 Subject: [Histonet] Manual photography and Picky Pathologistsl In-Reply-To: References: Message-ID: <93B5DD7E-8C08-413F-AE2D-7A9C049BF5B3@mcclainlab.com> Picky pathologists only want the best. They are a necessary evil. The problem is defining objective problems as opposed the perceived colors. To simplify the discussion, assume for the moment your pathologist has been evaluated and is NOT colorblind. To take the emotions and subjectivity out of defining what is acceptable color, one method we have used over the years involves manual photography and printing. Print the images full-page size. Have the pathologist photograph one acceptable section with the camera set to manual exposure and print that image. Next, WITHOUT changing lenses or camera settings, photograph and print the offending sections on the offending slide. The camera must be set to manual. Have the pathologist write down all camera and microscope settings. And repeat the following day. Using the scope and camera as a spectrophotometer tool for sorting out staining issues from perceptual issues. The end result of our evaluation was that we found Treosin by Statlab to be readily photographable. Steve A. McClain, MD Ref Histopathologic staining of low temperature cutaneous burns: comparing biomarkers of epithelial and vascular injury reveals utility of HMGB1 and hematoxylin phloxine saffron. Hirth DA, et al. Wound Repair Regen. 2012 Nov-Dec. Authors Hirth DA1, Singer AJ, Clark RA, McClain SA. Author information * 1School of Medicine, Stony Brook University, Stony Brook, New York, USA. Citation Wound Repair Regen. 2012 Nov-Dec;20(6):918-27. doi: 10.1111/j.1524-475X.2012.00847.x. From Fawn.Bomar at HalifaxRegional.com Wed Jul 15 12:31:30 2015 From: Fawn.Bomar at HalifaxRegional.com (Fawn Bomar) Date: Wed, 15 Jul 2015 17:31:30 +0000 Subject: [Histonet] H&E stainers Message-ID: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA05E45B@EXCH-2K10.hrhs.com> Hi everyone, I know this question has been asked before but I am having difficulty finding it. We are in the process of finding a new H&E stainer and was wondering what everyone would recommend or not recommend. We are a small facility- stain less than 100 H&E slides a day. We are also looking for one that is compatible with a coverslipper. Right now my manager is looking at the Sakura Prisma but we would have to do some major reconstruction to fit it in our lab. We are also looking at the Gemini with the Clearvue coverslipper. Can anyone give me their opinions on what they think? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From THicks at gulfcoastdermpath.com Wed Jul 15 12:42:12 2015 From: THicks at gulfcoastdermpath.com (Trish Hicks) Date: Wed, 15 Jul 2015 17:42:12 +0000 Subject: [Histonet] Medical necessity question Message-ID: <96D42D0E9FB88A46A1B3E3E82DE4CA9A5627AC33@GCD-EXCH.tampa.gcd.local> We are under the impression that we will be required to start putting a "reason" that a stain or IHC stain was performed in the microscopic description, or somewhere, for CMS to consider paying for an additional stain. It is a requirement that is upcoming and we want to be proactive. How is everyone else handling this or is what already appears in the microscopic description or diagnosis sufficient? Thank You, Trish Hicks BS, HTL (ASCP) Laboratory Supervisor Gulf Coast Dermatopathology Laboratory, Inc. Ph: 813-882-4206 Fax: 813-886-0589 Toll Free: 877-654-7721 thicks at gulfcoastdermpath.com Confidentiality: The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of this information by persons or entities other than the intended recipient(s) is unauthorized and prohibited. Any transmission of confidential and/or privileged material to persons or entities other than the intended recipient(s) shall not be construed as a waiver of any privilege or confidence. If you receive this transmission in error, please contact the sender and delete the material. Virus Disclaimer: The contents of an attachment to this e-mail may contain software viruses, which could damage your computer system. We cannot accept liability for any damage you may sustain as a result of software viruses. You should carry out your own virus checks before opening any attachment. From relia1 at earthlink.net Wed Jul 15 13:32:10 2015 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 15 Jul 2015 14:32:10 -0400 Subject: [Histonet] RELIA HOT HOT HOT Histology Job ALERT A RELIA EXCLUSIVE Histology Lab Manager - Dallas, TX Message-ID: <137501d0bf2c$903764c0$b0a62e40$@earthlink.net> Hi Histonetters! I am so excited about this opportunity that I had to write you about it right away. It is a RELIA EXCLUSIVE!! Here's the info: Histology Manager - Dallas, TX RELIA Solutions has been engaged exclusively to recruit a histology manager for an expanding lab located in Dallas, TX. This is a full time permanent DAY shift position. My client offers an EXCELLENT salary, great benefits and relocation assistance. Best of all there is a team of AMAZING techs waiting to be led by YOU! ASCP certification and 3-5 years of experience supervising a histology lab and it's staff is required for the position. For more information please contact Pam Barker at 866-607-3542 or relia1 at earthlink.net Keywords: histology, histotechnician, histotechnologist, histologist If you or anyone you know is interested please contact me. Remember if I place someone you refer to me I will pay you a referral fee. As a matter of fact I was writing referral reward checks this week!! I love to write referral reward checks almost as much as I love finding great opportunities for my histopeeps!! Stay tuned for some more exciting opportunities. Have a great day - We are halfway to the weekend as the camel says. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From ASelf at tidelandshealth.org Wed Jul 15 13:40:41 2015 From: ASelf at tidelandshealth.org (Amy Self) Date: Wed, 15 Jul 2015 14:40:41 -0400 Subject: [Histonet] test Message-ID: Test :) Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From kiran_g at sbcglobal.net Wed Jul 15 19:11:51 2015 From: kiran_g at sbcglobal.net (Kiranjit Grewal) Date: Wed, 15 Jul 2015 17:11:51 -0700 Subject: [Histonet] Fw: Re: : Ventana Retic Stain Quality Message-ID: <1437005511.54911.YahooMailBasic@web184805.mail.gq1.yahoo.com> Dear All, Anyone having issues with retic stain? We have seen inconsistent staining for months without any resolution. Any feedback on this will be helpful. thanks in advance, Kiran -------------------------------------------- From bburnett at CapeCodHealth.org Thu Jul 16 09:05:33 2015 From: bburnett at CapeCodHealth.org (Burnett, Brandy) Date: Thu, 16 Jul 2015 14:05:33 +0000 Subject: [Histonet] Ventana LCS (Liquid Cover Slip)/ non-specific staining In-Reply-To: References: Message-ID: Just wanted to thank everyone for all the helpful input in regards to the Ventana BenchMark Ultra. We are seeing some non-specific background staining on our slides. It is not so much an issue for our nuclear/membranous stains, however for our cytoplasmic stains, it could be interpreted as a false positive. We bake our slides offline at 65 degrees Celsius for 30 minutes, then Depar online (EZ Prep) at 72 degrees Celsius. Any feedback would be greatly appreciated! Thanks again, Brandy Burnett Histotechnologist, QIHC(ASCP) CCH Pathology/Histology bburnett at capecodhealth.org Expert physicians. Quality hospitals. Superior care. -----Original Message----- From: Burnett, Brandy [mailto:bburnett at CapeCodHealth.org] Sent: Thursday, July 02, 2015 8:32 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Ventana LCS (Liquid Cover Slip) Anyone out there having any issues with the Ventana LCS? When you are cover slipping are you seeing any water/residual LCS on the slides? I was wondering if we should dry our slides before cover slipping instead of running them down? Any information would be greatly appreciated! Brandy Burnett Histotechnologist, QIHC(ASCP) CCH Pathology/Histology bburnett at CapeCodHealth.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk at CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk at CapeCodHealth.org From bcdukes at lexhealth.org Thu Jul 16 12:14:34 2015 From: bcdukes at lexhealth.org (Blake Taylor) Date: Thu, 16 Jul 2015 17:14:34 +0000 Subject: [Histonet] Ventana Retic Stain Quality Message-ID: Are you using the Benchmark Special Stainer? If so it is due to a containment in the wash solution. Ventana knows they have a problem and are currently beta testing a new wash solution at other sites that should be rolled out soon. Of course they said soon 6-9 months ago. Anytime our Retic starts to have an issue we have to decon the machine. We have had our technical specialist in here numerous times and they are fully aware. -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, July 16, 2015 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 140, Issue 18 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. H&E stainers (Fawn Bomar) 2. Medical necessity question (Trish Hicks) 3. RELIA HOT HOT HOT Histology Job ALERT A RELIA EXCLUSIVE Histology Lab Manager - Dallas, TX (Pam Barker) 4. test (Amy Self) 5. Fw: Re: : Ventana Retic Stain Quality (Kiranjit Grewal) 6. Re: Ventana LCS (Liquid Cover Slip)/ non-specific staining (Burnett, Brandy) ---------------------------------------------------------------------- Message: 1 Date: Wed, 15 Jul 2015 17:31:30 +0000 From: Fawn Bomar To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] H&E stainers Message-ID: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA05E45B at EXCH-2K10.hrhs.com> Content-Type: text/plain; charset="iso-8859-1" Hi everyone, I know this question has been asked before but I am having difficulty finding it. We are in the process of finding a new H&E stainer and was wondering what everyone would recommend or not recommend. We are a small facility- stain less than 100 H&E slides a day. We are also looking for one that is compatible with a coverslipper. Right now my manager is looking at the Sakura Prisma but we would have to do some major reconstruction to fit it in our lab. We are also looking at the Gemini with the Clearvue coverslipper. Can anyone give me their opinions on what they think? Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ------------------------------ Message: 2 Date: Wed, 15 Jul 2015 17:42:12 +0000 From: Trish Hicks To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Medical necessity question Message-ID: <96D42D0E9FB88A46A1B3E3E82DE4CA9A5627AC33 at GCD-EXCH.tampa.gcd.local> Content-Type: text/plain; charset="us-ascii" We are under the impression that we will be required to start putting a "reason" that a stain or IHC stain was performed in the microscopic description, or somewhere, for CMS to consider paying for an additional stain. It is a requirement that is upcoming and we want to be proactive. How is everyone else handling this or is what already appears in the microscopic description or diagnosis sufficient? Thank You, Trish Hicks BS, HTL (ASCP) Laboratory Supervisor Gulf Coast Dermatopathology Laboratory, Inc. Ph: 813-882-4206 Fax: 813-886-0589 Toll Free: 877-654-7721 thicks at gulfcoastdermpath.com Confidentiality: The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of this information by persons or entities other than the intended recipient(s) is unauthorized and prohibited. Any transmission of confidential and/or privileged material to persons or entities other than the intended recipient(s) shall not be construed as a waiver of any privilege or confidence. If you receive this transmission in error, please contact the sender and delete the material. Virus Disclaimer: The contents of an attachment to this e-mail may contain software viruses, which could damage your computer system. We cannot accept liability for any damage you may sustain as a result of software viruses. You should carry out your own virus checks before opening any attachment. ------------------------------ Message: 3 Date: Wed, 15 Jul 2015 14:32:10 -0400 From: "Pam Barker" To: "Histonet" Subject: [Histonet] RELIA HOT HOT HOT Histology Job ALERT A RELIA EXCLUSIVE Histology Lab Manager - Dallas, TX Message-ID: <137501d0bf2c$903764c0$b0a62e40$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters! I am so excited about this opportunity that I had to write you about it right away. It is a RELIA EXCLUSIVE!! Here's the info: Histology Manager - Dallas, TX RELIA Solutions has been engaged exclusively to recruit a histology manager for an expanding lab located in Dallas, TX. This is a full time permanent DAY shift position. My client offers an EXCELLENT salary, great benefits and relocation assistance. Best of all there is a team of AMAZING techs waiting to be led by YOU! ASCP certification and 3-5 years of experience supervising a histology lab and it's staff is required for the position. For more information please contact Pam Barker at 866-607-3542 or relia1 at earthlink.net Keywords: histology, histotechnician, histotechnologist, histologist If you or anyone you know is interested please contact me. Remember if I place someone you refer to me I will pay you a referral fee. As a matter of fact I was writing referral reward checks this week!! I love to write referral reward checks almost as much as I love finding great opportunities for my histopeeps!! Stay tuned for some more exciting opportunities. Have a great day - We are halfway to the weekend as the camel says. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 4 Date: Wed, 15 Jul 2015 14:40:41 -0400 From: Amy Self To: "Histonet at lists.utsouthwestern.edu" Subject: [Histonet] test Message-ID: Content-Type: text/plain; charset="us-ascii" Test :) Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ------------------------------ Message: 5 Date: Wed, 15 Jul 2015 17:11:51 -0700 From: Kiranjit Grewal To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fw: Re: : Ventana Retic Stain Quality Message-ID: <1437005511.54911.YahooMailBasic at web184805.mail.gq1.yahoo.com> Content-Type: text/plain; charset=us-ascii Dear All, Anyone having issues with retic stain? We have seen inconsistent staining for months without any resolution. Any feedback on this will be helpful. thanks in advance, Kiran -------------------------------------------- ------------------------------ Message: 6 Date: Thu, 16 Jul 2015 14:05:33 +0000 From: "Burnett, Brandy" To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Ventana LCS (Liquid Cover Slip)/ non-specific staining Message-ID: Content-Type: text/plain; charset="us-ascii" Just wanted to thank everyone for all the helpful input in regards to the Ventana BenchMark Ultra. We are seeing some non-specific background staining on our slides. It is not so much an issue for our nuclear/membranous stains, however for our cytoplasmic stains, it could be interpreted as a false positive. We bake our slides offline at 65 degrees Celsius for 30 minutes, then Depar online (EZ Prep) at 72 degrees Celsius. Any feedback would be greatly appreciated! Thanks again, Brandy Burnett Histotechnologist, QIHC(ASCP) CCH Pathology/Histology bburnett at capecodhealth.org Expert physicians. Quality hospitals. Superior care. -----Original Message----- From: Burnett, Brandy [mailto:bburnett at CapeCodHealth.org] Sent: Thursday, July 02, 2015 8:32 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Ventana LCS (Liquid Cover Slip) Anyone out there having any issues with the Ventana LCS? When you are cover slipping are you seeing any water/residual LCS on the slides? I was wondering if we should dry our slides before cover slipping instead of running them down? Any information would be greatly appreciated! Brandy Burnett Histotechnologist, QIHC(ASCP) CCH Pathology/Histology bburnett at CapeCodHealth.org ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk at CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk at CapeCodHealth.org ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 140, Issue 18 ***************************************** PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. From Fawn.Bomar at HalifaxRegional.com Thu Jul 16 12:57:51 2015 From: Fawn.Bomar at HalifaxRegional.com (Fawn Bomar) Date: Thu, 16 Jul 2015 17:57:51 +0000 Subject: [Histonet] Stainer Message-ID: <35B63A2E2FC1C8429D3ACF1CDDA5FFCA05E577@EXCH-2K10.hrhs.com> Thank you everyone for your input! So far most people like the Sakura, the hard part is figuring out where to fit it. Thank you Fawn ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you From tbraud at holyredeemer.com Thu Jul 16 13:20:08 2015 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 16 Jul 2015 18:20:08 +0000 Subject: [Histonet] H&E stainers and LCS In-Reply-To: References: Message-ID: <48E053DDF6CE074DB6A7414BA05403F8020A03@HRHEX02-HOS.holyredeemer.local> H&E Stainer - I love our Prisma and Glas coverslipper. Such a workhorse. Easy maintenance. Almost never any downtime. LCS - Liquid coverslip is a type of oil, and should rinse easily in your alcohols during run down. If you are seeing water, perhaps it is held in place with your slide label, and your alcohols are not high enough to properly dehydrate the slides. We had a similar issue and that was what we encountered. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 ************** From patpxs at gmail.com Thu Jul 16 13:55:15 2015 From: patpxs at gmail.com (Patpxs) Date: Thu, 16 Jul 2015 11:55:15 -0700 Subject: [Histonet] Ventana Retic Stain Quality In-Reply-To: References: Message-ID: <24B58F7D-7ED8-49EE-A102-6622D27031D0@gmail.com> I was told by the technical specialist to make the wash solution fresh before use. There has been a contamination caused by growth of organisms in the wash if it sits around too long between uses. Paula :-) Sent from my iPhone > On Jul 16, 2015, at 10:14 AM, Blake Taylor via Histonet wrote: > > Are you using the Benchmark Special Stainer? If so it is due to a containment in the wash solution. Ventana knows they have a problem and are currently beta testing a new wash solution at other sites that should be rolled out soon. Of course they said soon 6-9 months ago. Anytime our Retic starts to have an issue we have to decon the machine. We have had our technical specialist in here numerous times and they are fully aware. > > > > -----Original Message----- > From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] > Sent: Thursday, July 16, 2015 1:00 PM > To: histonet at lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 140, Issue 18 > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. H&E stainers (Fawn Bomar) > 2. Medical necessity question (Trish Hicks) > 3. RELIA HOT HOT HOT Histology Job ALERT A RELIA EXCLUSIVE > Histology Lab Manager - Dallas, TX (Pam Barker) > 4. test (Amy Self) > 5. Fw: Re: : Ventana Retic Stain Quality (Kiranjit Grewal) > 6. Re: Ventana LCS (Liquid Cover Slip)/ non-specific staining > (Burnett, Brandy) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 15 Jul 2015 17:31:30 +0000 > From: Fawn Bomar > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] H&E stainers > Message-ID: > <35B63A2E2FC1C8429D3ACF1CDDA5FFCA05E45B at EXCH-2K10.hrhs.com> > Content-Type: text/plain; charset="iso-8859-1" > > Hi everyone, > > > > I know this question has been asked before but I am having difficulty finding it. We are in the process of finding a new H&E stainer and was wondering what everyone would recommend or not recommend. We are a small facility- stain less than 100 H&E slides a day. We are also looking for one that is compatible with a coverslipper. Right now my manager is looking at the Sakura Prisma but we would have to do some major reconstruction to fit it in our lab. We are also looking at the Gemini with the Clearvue coverslipper. Can anyone give me their opinions on what they think? > > > > Thank you > > Fawn > ------------------------------------------------------------- > This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. > > If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. > > Thank you > > > ------------------------------ > > Message: 2 > Date: Wed, 15 Jul 2015 17:42:12 +0000 > From: Trish Hicks > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Medical necessity question > Message-ID: > <96D42D0E9FB88A46A1B3E3E82DE4CA9A5627AC33 at GCD-EXCH.tampa.gcd.local> > Content-Type: text/plain; charset="us-ascii" > > We are under the impression that we will be required to start putting a "reason" that a stain or IHC stain was performed in the microscopic description, or somewhere, for CMS to consider paying for an additional stain. It is a requirement that is upcoming and we want to be proactive. How is everyone else handling this or is what already appears in the microscopic description or diagnosis sufficient? > > Thank You, > > Trish Hicks > BS, HTL (ASCP) > Laboratory Supervisor > Gulf Coast Dermatopathology Laboratory, Inc. > Ph: 813-882-4206 > Fax: 813-886-0589 > Toll Free: 877-654-7721 > thicks at gulfcoastdermpath.com > Confidentiality: The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of this information by persons or entities other than the intended recipient(s) is unauthorized and prohibited. Any transmission of confidential and/or privileged material to persons or entities other than the intended recipient(s) shall not be construed as a waiver of any privilege or confidence. If you receive this transmission in error, please contact the sender and delete the material. > Virus Disclaimer: The contents of an attachment to this e-mail may contain software viruses, which could damage your computer system. We cannot accept liability for any damage you may sustain as a result of software viruses. You should carry out your own virus checks before opening any attachment. > > > > > > ------------------------------ > > Message: 3 > Date: Wed, 15 Jul 2015 14:32:10 -0400 > From: "Pam Barker" > To: "Histonet" > Subject: [Histonet] RELIA HOT HOT HOT Histology Job ALERT A RELIA > EXCLUSIVE Histology Lab Manager - Dallas, TX > Message-ID: <137501d0bf2c$903764c0$b0a62e40$@earthlink.net> > Content-Type: text/plain; charset="us-ascii" > > Hi Histonetters! > I am so excited about this opportunity that I had to write you about it right away. > It is a RELIA EXCLUSIVE!! > Here's the info: > Histology Manager - Dallas, TX > RELIA Solutions has been engaged exclusively to recruit a histology manager for an expanding lab located in Dallas, TX. This is a full time permanent DAY shift position. My client offers an EXCELLENT salary, great benefits and relocation assistance. Best of all there is a team of AMAZING techs waiting to be led by YOU! ASCP certification and 3-5 years of experience supervising a histology lab and it's staff is required for the position. > For more information please contact Pam Barker at 866-607-3542 or relia1 at earthlink.net > Keywords: histology, histotechnician, histotechnologist, histologist If you or anyone you know is interested please contact me. Remember if I place someone you refer to me I will pay you a referral fee. As a matter of fact I was writing referral reward checks this week!! I love to write referral reward checks almost as much as I love finding great opportunities for my histopeeps!! > Stay tuned for some more exciting opportunities. > Have a great day - We are halfway to the weekend as the camel says. > > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > Pam M. Barker > > Pam Barker > President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1 at earthlink.net > www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > > > > > > > > ------------------------------ > > Message: 4 > Date: Wed, 15 Jul 2015 14:40:41 -0400 > From: Amy Self > To: "Histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] test > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Test :) > > Amy Self > Histology Lab Senior Tech > Lab > Tidelands Georgetown Memorial Hospital > 606 Black River Road > Georgetown, SC 29440 > 843-520-8711 > ASelf at tidelandshealth.org > > NOTE: > The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > > > ------------------------------ > > Message: 5 > Date: Wed, 15 Jul 2015 17:11:51 -0700 > From: Kiranjit Grewal > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Fw: Re: : Ventana Retic Stain Quality > Message-ID: > <1437005511.54911.YahooMailBasic at web184805.mail.gq1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > > Dear All, > > Anyone having issues with retic stain? We have seen inconsistent staining for months without any resolution. > Any feedback on this will be helpful. > thanks in advance, Kiran > -------------------------------------------- > > > > ------------------------------ > > Message: 6 > Date: Thu, 16 Jul 2015 14:05:33 +0000 > From: "Burnett, Brandy" > To: "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Ventana LCS (Liquid Cover Slip)/ non-specific > staining > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Just wanted to thank everyone for all the helpful input in regards to the Ventana BenchMark Ultra. > We are seeing some non-specific background staining on our slides. It is not so much an issue for > our nuclear/membranous stains, however for our cytoplasmic stains, it could be interpreted as a false > positive. We bake our slides offline at 65 degrees Celsius for 30 minutes, then Depar online (EZ Prep) at 72 degrees > Celsius. Any feedback would be greatly appreciated! > > Thanks again, > > Brandy Burnett > Histotechnologist, QIHC(ASCP) > CCH Pathology/Histology > bburnett at capecodhealth.org > > > Expert physicians. Quality hospitals. Superior care. > > -----Original Message----- > From: Burnett, Brandy [mailto:bburnett at CapeCodHealth.org] > Sent: Thursday, July 02, 2015 8:32 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Ventana LCS (Liquid Cover Slip) > > Anyone out there having any issues with the Ventana LCS? > When you are cover slipping are you seeing any water/residual LCS on the slides? > I was wondering if we should dry our slides before cover slipping instead of running them down? Any information would be greatly appreciated! > > Brandy Burnett > Histotechnologist, QIHC(ASCP) > CCH Pathology/Histology > bburnett at CapeCodHealth.org > > > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ > This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. > > Helpdesk at CapeCodHealth.org > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ > This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. > > Helpdesk at CapeCodHealth.org > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 140, Issue 18 > ***************************************** > PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick at northwestern.edu Fri Jul 17 07:53:28 2015 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Fri, 17 Jul 2015 12:53:28 +0000 Subject: [Histonet] Masson's on Frozens. Message-ID: <083378e5d22f41d495220a69166ca053@evcspmbx03.ads.northwestern.edu> Question: When we stain and H&E on frozen we fix in 95% prior to staining. As I never in 30 years have done a Masson's Trichrome on a frozen section I ask : do I need to fix and then mordant as usual or go straight to the Bouin's and continue as normal? Do I need to mordant at all? Tissue is rabbit liver. BErnice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-frederick at northwestern.edu From FMonson at wcupa.edu Fri Jul 17 08:37:30 2015 From: FMonson at wcupa.edu (Monson, Frederick) Date: Fri, 17 Jul 2015 13:37:30 +0000 Subject: [Histonet] AFB contaminant - OR - Monkish Preparation In-Reply-To: <852F7D2C14FB464D80E182B15DB138AF6B7E72E9@CHIEX005.CHI.catholichealth.net> References: <852F7D2C14FB464D80E182B15DB138AF6B7E72E9@CHIEX005.CHI.catholichealth.net> Message-ID: <6dd98048c82a496985124e260ac041f7@WCUXCHP08.PASSHE.LCL> I slways filtered my air (5um) and my water (0.22um). Only tap water rinses for Hemnatoxylin, Schiff's and other rinses were not rinsed. All of my MolBio and Immuno- stuff was done with filtered (clean?) water at least. The rule is: be Monkish at the bench and as messy as you like in your bedroom (if you are a male and only until you are married), Cheers, Fred Inside your water supply, lurking behind the water fawcet, is a pipe who's surface is lined with a BIO-film. Further horrors await the scientist who fixes and studies the washer in the fawcet whose seal is the better for the 'FILM-mmmmmmmmm.' Evolution provides variation, and every new flower that grows provides a new isolated environment for speciation. My alimentary biome is unique as we are all unique. Yukky, but true. Frederick C Monson, PhD Center for Microanalysis and Imaging, Research and Training (CMIRT) West Chester University of Pannsylvania West Chester, PA, 1938 610-738-0437 fmonson at wcupa.edu ________________________________________ From: Abbott, Tanya Sent: Thursday, July 9, 2015 3:09 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] AFB contaminant Has anyone ever had a problem with a possible contaminant in their AFB hand stain? If so, how did you deal with it? Any suggestions? Ours actually looked like an AFB like organism, which we later determined to be negative. Thanks! Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TanyaAbbott at catholichealth.net Fri Jul 17 09:48:17 2015 From: TanyaAbbott at catholichealth.net (Abbott, Tanya) Date: Fri, 17 Jul 2015 14:48:17 +0000 Subject: [Histonet] IHC negative controls Message-ID: <852F7D2C14FB464D80E182B15DB138AF6B7ECCC8@CHIEX005.CHI.catholichealth.net> Happy Friday everyone! We currently have a Ventana Ultra. I am wondering what everyone is doing/running for a negative reagent control and/or a negative tissue control? Thanks in advance! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology St Joseph Medical Center (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From cjbulmer2526 at aol.com Fri Jul 17 10:06:16 2015 From: cjbulmer2526 at aol.com (Cindy Bulmer) Date: Fri, 17 Jul 2015 11:06:16 -0400 Subject: [Histonet] Test Message-ID: <14e9c8d3345-7394-17bb9@webprd-m55.mail.aol.com> Test Cynthia Bulmer HT(ASCP),QIHC IHC Supervisor, CTPL Waco, TX From thigginsht at msn.com Fri Jul 17 12:48:14 2015 From: thigginsht at msn.com (Tim H) Date: Fri, 17 Jul 2015 12:48:14 -0500 Subject: [Histonet] IHC negative controls In-Reply-To: References: Message-ID: We run patient tissue as a negative and we use diluent as the reagent negative. TH > Message: 7 > Date: Fri, 17 Jul 2015 14:48:17 +0000 > From: "Abbott, Tanya" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] IHC negative controls > Message-ID: > <852F7D2C14FB464D80E182B15DB138AF6B7ECCC8 at CHIEX005.CHI.catholichealth.net> > > Content-Type: text/plain; charset="us-ascii" > > Happy Friday everyone! We currently have a Ventana Ultra. I am wondering what everyone is doing/running for a negative reagent control and/or a negative tissue control? > Thanks in advance! > Tanya > > Tanya G. Abbott > Manager Technologist > Histology/Cytology > St Joseph Medical Center > (phone) 610-378-2635 > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From wdgill04 at gmail.com Fri Jul 17 17:42:37 2015 From: wdgill04 at gmail.com (Wayne Gill) Date: Fri, 17 Jul 2015 17:42:37 -0500 Subject: [Histonet] Waste disposal of histology chemicals in Texas Cameron County Message-ID: Hello histo people, I am trying to get a new small histology lab together here and I'm new to the state of Texas and to Cameron County. I lived in Virgina before for a private company and a couple community hospitals before moving here. In Virgina we neutralized our formalin waste and once it was neurtalized we poured it down the sink with lots of water. Can we do that in Texas (Cameron county)?? Where could I find info on histology wastes? I will have clear-rite, alcohols, small quantities of xylene, and some special stains probably. Can stuff like paraffin go in the regular trash and a waste disposal company take all the rest like alcohol, clear-rite, and special stain waste? Wayne Gill, HTL(ASCP)CM From rsrichmond at gmail.com Fri Jul 17 19:01:54 2015 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 17 Jul 2015 20:01:54 -0400 Subject: [Histonet] Masson's on Frozens. Message-ID: Bernice Frederick HTL (ASCP) at Northwestern in Chicago USA asks: >>When we stain and H&E on frozen [sections] we fix in 95% prior to staining. As I never in 30 years have done a Masson's Trichrome on a frozen section I ask: do I need to fix and then mordant as usual or go straight to the Bouin's and continue as normal? Do I need to mordant at all? Tissue is rabbit liver.<< Are you communicating with your pathologist? What are you trying to do? The one-step trichrome techniques associated with the hallowed name of George Gomori (particularly the Engel-Cunningham variant) are probably preferable for frozen sections - they certainly are for skeletal muscle - where I have a lot of experience, though many years ago. But find out what's wanted. Bob Richmond Samurai Pathologist Maryville TN From gayle.callis at bresnan.net Sun Jul 19 11:57:52 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Sun, 19 Jul 2015 10:57:52 -0600 Subject: [Histonet] mouse leg decalcification Message-ID: <000301d0c244$0d0bb4a0$27231de0$@bresnan.net> Steven, The advice Iliana gave was excellent. We avoided HCl decalcifiers for any bone decalcification due to the damaging effects on staining (H&E, Giemsa) but more importantly on antigens if you plan on immunostaining. You did NOT say what you what kind of staining you wanted with murine jaws and leg bones? Please enlighten us about what you need post decalcification. Buffered formic acid i.e. Cal Ex II is a good choice but with any acid decalcifier and there are other commercial buffered formic acid reagents available so check the MSDS to see how they are made. You should do a decalcifcation endpoint check to not over expose the tissues/cells, etc to acids, even this gentler buffered formic acid. My EDTA decalcification method does not take two months to be complete and I will be happy to send you this method from a publication by Dr. Webb Jee many years ago. This is 14% tetra sodium EDTA, pH 7.6 dissolved in Dulbeccos PBS or distilled water. This particular EDTA is very alkaline(pH 11) when dissolved and the pH must be adjusted down using acetic acid, not HCl. Very alkaline EDTA from pH 8 to pH 11 can potentially damage alkaline sensitive protein linkages so we stay with the pH commonly used with TRIS Buffered saline, making final decalcified bone safe for immunostaining. EDTA decalcification acts as a function of pH, so that decalcification is slower at pH 7 than at pH 7.6. This is a chemical fact explained in text books. We do endpoint checks faithfully for any and all bone decalcification. The most sensitive methods are with an xray (Faxitron) or Micro CT units if you have these available. The weight loss/weight gain method is easy, cheap and but you need a balance that weighs in mg units, preferably three places. I have used the weight gain/weight loss method for years and did it daily with all acid decalcifying agents, but you can do it less often with EDTA. Bones were immersed in formic acid decalcify solution in late afternoon and do the test the next morning since some leg bones will almost be totally done by the end of the next day. We suspend bones in nylon biopsy bags and stir or rock the container of decalcifying solution during decalcification to release the CO2 bubbles collecting on the bone surface which then causes bones to float upwards. CO2 is given off as part of the acid decalcification chemical process. We make sure the bones are totally fixed for 4 days or longer to protect from the effects of acid i.e. bad nuclear staining, damage to antigens, BEFORE ever going into any decalcifying agent. We normally do a 10% - 15% formic acid made up from 88 - 90% stock formic acid in the lab although buying commercial buffered formic acid. Buffered formic acids are approximately 4.5% formic acid with either sodium formate or sodium citrate buffering salts. They can take a bit longer but murine bones aren't very large. We have done whole mouse and whole rat skulls in the past with perfect H&E staining after using total NBF fixation and 10% formic acid decalcification using endpoint checks daily. The key is to NBF fix totally, do endpoint check to ensure removal of calcium and use either a formic acid decalcifying solution. Good luck Gayle Callis HTL/HT/MT(ASCP) You wrote: We do mouse legs routinely, either with Cal Ex II or EDTA. We look at the bone and the tissue around, so we have to have good fixation, decal., cutting and staining for both. Cal Ex II (formic acid) is a gentler decal agent than HCL (Plain "Cal EX") - and Cal Ex II keeps the H&E staining true - whereas HCL would make the Eosin dominate and stain everything! For future - since Cal Ex II contains Formalin - on theory you can fix and decal at the same time, but I always do my fixation first and then decal after. Use chemical method to determine the end point. Other ways to decal bone are with EDTA - I personally prefer it, though it takes a really long time, almost 2 months, it is better if you want to do IHC for certain markers that the acids might interfere with. We weight the samples before we start decal, and after that 2/week. The moment you stop seeing decrease in weight and see slight increase, that is the end point. For cutting, I prefer samples decalc. with Cal Ex II, cuts without a problem. Iliana Dimitrova, MLT, LHP, B.Tech., M.Sc. Histology Supervisor Medical Education and Laboratory Support Services (MELSS) Faculty of Medicine Memorial University of Newfoundland St. John's, NL Canada A1B 3V6 -----Original Message----- From: Swartwood, Steven J [mailto:Steven.Swartwood at cshs.org ] Sent: July-10-15 7:23 PM To: 'histonet at lists.utsouthwestern.edu ' Subject: [Histonet] Decalcification of mouse jaw and legs Hello all, I hope everyone is having or had a fantastic week, I'm going to test a few different decal methods on mouse jaws and leg bones. I was wondering if anyone does this routinely out there who is willing to share a protocol. I've never done this on mouse jaw/leg bones before. I've only done this on human tissues. From what I've read it seems that for routine histology low % HCl is fine, but if I wanted to perform IHC then anywhere from 5-15% formic acid may be the best way to go. Nitric acid is probably too strong of a decal agent for these tiny specimens I would assume. Maybe an EDTA based decal agent may be best as well. I'm just really inexperienced with this and I'm very open to ideas and trying a few different methods. Steven Swartwood HT(ASCP) From SteveM at mcclainlab.com Sun Jul 19 14:00:38 2015 From: SteveM at mcclainlab.com (Steve McClain) Date: Sun, 19 Jul 2015 19:00:38 +0000 Subject: [Histonet] Decalcification In-Reply-To: References: Message-ID: One frequently overlooked point is washing after decal. Especially important with acid decal, we wash for an hour in running water. From Pamela.S.Younes at uth.tmc.edu Mon Jul 20 08:00:06 2015 From: Pamela.S.Younes at uth.tmc.edu (Younes, Pamela S) Date: Mon, 20 Jul 2015 13:00:06 +0000 Subject: [Histonet] Cerner PathNet AP tracking Message-ID: Hello everyone, Has anyone used the Cerner PathNet AP tracking system? I would like to hear about any feedback, good or bad. Many thanks, Pam Younes Pamela S. Younes MHS, HTL(ASCP), CPC(AAPC), PA(ASCP) Pathologists' Assistant Assistant Professor, Department of Pathology and Laboratory Medicine University of Texas Medical School, Houston 6430 Fannin, MSB 2.233 Houston, TX 77030 (713) 704-2053 From thomas.huynh at mdanderson.org Mon Jul 20 13:00:16 2015 From: thomas.huynh at mdanderson.org (Huynh,Thomas) Date: Mon, 20 Jul 2015 18:00:16 +0000 Subject: [Histonet] Decalcification Message-ID: Hello Histoneters, I would like to add that washing after decalcifying is still not enough. The bone specimens have to be neutralized before processing ( putting them in the neutralizer solution), so there are no trace of acid when we process them. Thomas -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Monday, July 20, 2015 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 140, Issue 22 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Decalcification (Steve McClain) 2. Cerner PathNet AP tracking (Younes, Pamela S) ---------------------------------------------------------------------- Message: 1 Date: Sun, 19 Jul 2015 19:00:38 +0000 From: Steve McClain To: "" Subject: [Histonet] Decalcification Message-ID: Content-Type: text/plain; charset="us-ascii" One frequently overlooked point is washing after decal. Especially important with acid decal, we wash for an hour in running water. ------------------------------ Message: 2 Date: Mon, 20 Jul 2015 13:00:06 +0000 From: "Younes, Pamela S" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Cerner PathNet AP tracking Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello everyone, Has anyone used the Cerner PathNet AP tracking system? I would like to hear about any feedback, good or bad. Many thanks, Pam Younes Pamela S. Younes MHS, HTL(ASCP), CPC(AAPC), PA(ASCP) Pathologists' Assistant Assistant Professor, Department of Pathology and Laboratory Medicine University of Texas Medical School, Houston 6430 Fannin, MSB 2.233 Houston, TX 77030 (713) 704-2053 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 140, Issue 22 ***************************************** From shannonegower at gmail.com Mon Jul 20 19:10:03 2015 From: shannonegower at gmail.com (Shannon Gower) Date: Mon, 20 Jul 2015 20:10:03 -0400 Subject: [Histonet] IHC negative controls Message-ID: <5316E0D7-FA7E-4AC6-B5C8-0AFD56664088@gmail.com> We purchased a Universal Negative reagent from Ventana and then we use that in a prep kit, totally eliminating the need for Mouse and Rabbit negatives and a lot of extra space on the carousel. Shannon Gower Senior Histotechnologist HTL(ASCP) All Children?s Hospital St. Petersburg, FL (727-767-3058) From shannonegower at gmail.com Mon Jul 20 19:18:43 2015 From: shannonegower at gmail.com (Shannon Gower) Date: Mon, 20 Jul 2015 20:18:43 -0400 Subject: [Histonet] FISH on paraffin in pediatrics? Message-ID: Hello Histo world, Is there anyone out there who is doing FISH on FFPE tissue in pediatric facilities? Seems most of the research is going in the direction of adults and I would like to incorporate some of these new testing procedures in our lab. Cytogenetics is getting busier and busier, Histology wants to compete too! Shannon Gower Senior Histotechnologist HTL(ASCP) All Children?s Hospital St. Petersburg, FL (727-767-3058) sgower1 at jhmi.edu From thisisann at aol.com Tue Jul 21 10:05:31 2015 From: thisisann at aol.com (Ann Specian) Date: Tue, 21 Jul 2015 11:05:31 -0400 Subject: [Histonet] Tissue processing validation Message-ID: <14eb125f480-49fc-eeb@webprd-a61.mail.aol.com> We are moving out Peloris tissue processors to another room, so we have to validate. I was wondering what other labs do as a validation for this type of move? Do you run tissue on every progam utilizing each processor for at least one of the protocols? Any help would be appreciated. thanks, Ann From Linda.Margraf at cookchildrens.org Tue Jul 21 10:54:29 2015 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Tue, 21 Jul 2015 15:54:29 +0000 Subject: [Histonet] email issues Message-ID: <928719B9EBFA1C4686918B975FF84528EE8885B2@CCHCSMBX04.CCHCS.LDAP> Dear Histonetters, I have heard from a couple of list members that they have been removed from the list from excess bounces and once they have gotten back on the listserv membership list, they no longer receive a copy of any message they post to the list. They use Yahoo and AOL. I contacted the IT experts at the university who investigated. It appears Yahoo and probably AOL have changed settings to improve spam filtering and it is blocking or bouncing mail. The IT guys changed some settings on the server. The bouncing issue seems to be resolved but for people using those email systems, they may not get a copy of any messages they post to the list any longer. Apparently all the other mail from the list comes through just fine. If that happens to you, you can check the archive (http://www.histosearch.com/histonet.html) to see if your message is there. There is also a setting for your Histonet subscription, that you can turn on, that will send an acknowledgement when you post to the list. I am testing this now as I don't think we have used it before. Yahoo and AOL (or other email providers) customer service may also be able to help. To change or look at your personal listserv settings on the list visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet Remember, if you get a message about being a non-member if you try to post a message, it means your email address has likely changed from when you first subscribed and it needs updating, which you can also do at this site. Thanks, Linda M Histonet administrator From SteveM at mcclainlab.com Tue Jul 21 13:06:58 2015 From: SteveM at mcclainlab.com (Steve McClain) Date: Tue, 21 Jul 2015 18:06:58 +0000 Subject: [Histonet] Decalcification and Neutralization (Huynh,Thomas) In-Reply-To: References: Message-ID: <99F0DF53-7453-483C-B28A-190431CC1F1B@mcclainlab.com> I have not found the need for neutralization. With sufficient washing, common histochemical stains including H&E Gram PAS-fungus and trichrome are spectacular. Please explain your protocol for neutralization. Steve A. McClain, MD From SteveM at mcclainlab.com Tue Jul 21 13:18:12 2015 From: SteveM at mcclainlab.com (Steve McClain) Date: Tue, 21 Jul 2015 18:18:12 +0000 Subject: [Histonet] Tissue processing validation (Ann Specian) In-Reply-To: References: Message-ID: <091A33CF-D1F9-4716-BB3E-429FA3250BED@mcclainlab.com> This seems like overkill. Just moving a processor previously validated should not require re-validation, just verify the programming and make certain the paraffins are melted completely. This may not apply to all processors, but for all Sakura machines manufactured since 1980,I would not revalidate. Steve A. McClain, MD From Linda.Margraf at cookchildrens.org Tue Jul 21 15:05:57 2015 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Tue, 21 Jul 2015 20:05:57 +0000 Subject: [Histonet] PLA2R on paraffin sections- IHC versus indirect immunofluorescence Message-ID: <928719B9EBFA1C4686918B975FF84528EE8886FB@CCHCSMBX04.CCHCS.LDAP> Here is a message I am posting for Martha....... My renal pathologists want to add this antibody and I want to see what methodologies everyone is using. The article I was reading mentioned both as an option. I would prefer to do IHC and run it on our Bond 3, as opposed to the very manual indirect IF method. Any feedback would be appreciated. Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC mward at wakehealth.edu From s.shah at garvan.org.au Tue Jul 21 17:49:23 2015 From: s.shah at garvan.org.au (Shruti Shah) Date: Tue, 21 Jul 2015 22:49:23 +0000 Subject: [Histonet] Bone histology charges Message-ID: Hi All, Can any one let me know general price for mouse bone decalcification, processing, microtome charges for per block or per slide. it will great help. Many thanks, NOTICE Please consider the environment before printing this email. This message and any attachments are intended for the addressee named and may contain legally privileged/confidential/copyright information. If you are not the intended recipient, you should not read, use, disclose, copy or distribute this communication. If you have received this message in error please notify us at once by return email and then delete both messages. We accept no liability for the distribution of viruses or similar in electronic communications. This notice should not be removed. From tony.henwood at health.nsw.gov.au Tue Jul 21 18:12:56 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue, 21 Jul 2015 23:12:56 +0000 Subject: [Histonet] Tissue processing validation In-Reply-To: <14eb125f480-49fc-eeb@webprd-a61.mail.aol.com> References: <14eb125f480-49fc-eeb@webprd-a61.mail.aol.com> Message-ID: <6D6BD1DE8A5571489398B392A38A71570197644138@xmdb04.nch.kids> The validation would already have been done. You only need to check if the re-location has affected any equipment components. I would take a few cassettes of spare tissue and process it on your most used program and await the results. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Ann Specian via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 22 July 2015 1:06 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Tissue processing validation We are moving out Peloris tissue processors to another room, so we have to validate. I was wondering what other labs do as a validation for this type of move? Do you run tissue on every progam utilizing each processor for at least one of the protocols? Any help would be appreciated. thanks, Ann _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From ASelf at tidelandshealth.org Wed Jul 22 07:03:15 2015 From: ASelf at tidelandshealth.org (Amy Self) Date: Wed, 22 Jul 2015 08:03:15 -0400 Subject: [Histonet] test again Message-ID: Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From relia1 at earthlink.net Wed Jul 22 12:13:38 2015 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 22 Jul 2015 13:13:38 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin 7-23-2015 How to stay cool as a cucumber when it's sizzling hot! Message-ID: <006201d0c4a1$c0919710$41b4c530$@earthlink.net> Hi Histonetters!! I hope you are having a great week. IT'S SO HOT!!!! I know you feel it! We all feel it! It's hot EVERYWHERE!!! So while you are doing your best to try and cool off here is something to think about. Did you know that the phrase Cool as a Cucumber originates from the fact that on a warm day a field cucumber has an internal temperature that is 20 degrees cooler than the temperature of the air! Chill a cucumber in the fridge and slice it up. **Add a few slices to your glass or jug of ice water. **Substitute cucumber slices for bread when you make sandwiches or add them to sandwiches for extra crunch. **Make popsicles with lime juice and chili. **Put a couple of slices on your eyelids and rest under a fan for a few minutes. **Toss some thinly sliced cucumber in plain yogurt, a drizzle of white wine vinegar and a teaspoon of dill for a refreshing side salad. Take advantage of "nature's chiller" to refresh and revive!! As you "chill out" take a moment and visualize the possibilities these opportunities inspire. As I was writing this email I realized that these are some of the most beautiful and picturesque areas of the U.S. Here is a list of the areas where I have clients who are looking for talented supervisors, lead techs and histology techs!! Asheville, NC Waynesboro, VA Great Falls, MT Santa Maria, CA Dallas, TX Atlanta, GA Seattle, WA Waco, TX If you have any questions or know someone who might be interested please contact me. I would love to tell you about these opportunities or write another referral check like I did today if you have a friend who might be interested. Remember it never hurts to look. Hope to hear from you soon! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From melissa at alliedsearchpartners.com Wed Jul 22 14:02:05 2015 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Wed, 22 Jul 2015 19:02:05 +0000 Subject: [Histonet] Histotech Job Opening in Aurora 4 days/wk 10hr Shifts Message-ID: Hello, I have a permanent position for a histotech interested in a Monday-Thursday 10 hour shifts position. Must be ASCP certified with 2+ years of experience. Please email or call me for more details. Melissa Owens (Phelan) President, Laboratory Staffing Allied Search Partners www.linkedin.com/in/melissaphelan/ http://www.alliedsearchpartners.com T: 888.388.7571 ext. 102 F: 888.388.7572 *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From modz9636 at gmail.com Wed Jul 22 15:24:09 2015 From: modz9636 at gmail.com (M.O.) Date: Wed, 22 Jul 2015 13:24:09 -0700 Subject: [Histonet] understanding reagents in decalcifier; making it in-house Message-ID: Hello Histonet, The supplier for our decalcifier, TBD-2 from Shandon, is having issues with getting the product out and we will not be receiving it for at least another month. Our samples are piling up and I don't know what I should do, but maybe I can make the decalcifier in-house. I am wondering if I can make my own based on the reagents they listed and their percentages and if certain reagents are not actually necessary. The samples we typically decalcify are mouse knees (decal time = 2 days), mouse spines (3 days), human bone slabs about 7mm in thickness (7-12 days). Fixation is in zinc buffered formalin, then decalcification, then 70% EtOH. Our choice to use TBD-2 is due to the gentle decalcification for IHC and we get GREAT results. Composition of Shandon TBD-2 Decalcifier: Component Weight % Water 77-80 Formic acid 21-23 Fluorad >1 Sodium citrate >1 Polyvinyl pyrrolidone >1 If you have any input on what reagents I should use and the percentages for making a decalcifier myself, it would be much appreciated! Thank you for you help, Merissa From rjbuesa at yahoo.com Wed Jul 22 15:39:58 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 22 Jul 2015 20:39:58 +0000 (UTC) Subject: [Histonet] understanding reagents in decalcifier; making it in-house In-Reply-To: References: Message-ID: <1613394726.663901.1437597598381.JavaMail.yahoo@mail.yahoo.com> If you can make the decalcifier with that recipe, you will be OK. You can even calculate costs and probably it will be cheaper than buying it from Shandon.Ren? On Wednesday, July 22, 2015 4:36 PM, M.O. via Histonet wrote: Hello Histonet, The supplier for our decalcifier, TBD-2 from Shandon, is having issues with getting the product out and we will not be receiving it for at least another month.? Our samples are piling up and I don't know what I should do, but maybe I can make the decalcifier in-house.? I am wondering if I can make my own based on the reagents they listed and their percentages and if certain reagents are not actually necessary. The samples we typically decalcify are mouse knees (decal time = 2 days), mouse spines (3 days), human bone slabs about 7mm in thickness (7-12 days).? Fixation is in zinc buffered formalin, then decalcification, then 70% EtOH.? Our choice to use TBD-2 is due to the gentle decalcification for IHC and we get GREAT results. Composition of Shandon TBD-2 Decalcifier: Component? ? ? ? ? ? ? ? Weight % ? ? Water? ? ? ? ? ? ? ? ? ? ? 77-80 Formic acid? ? ? ? ? ? ? ? 21-23 ? ? Fluorad? ? ? ? ? ? ? ? ? ? ? >1 Sodium citrate? ? ? ? ? ? ? >1 Polyvinyl pyrrolidone? ? ? >1 If you have any input on what reagents I should use and the percentages for making a decalcifier myself, it would be much appreciated! Thank you for you help, Merissa _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Wed Jul 22 16:29:37 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 22 Jul 2015 21:29:37 +0000 Subject: [Histonet] understanding reagents in decalcifier; making it in-house In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF60303D52@ex07.net.ucsf.edu> Merissa, Water 77-80 solvent Formic acid 21-23 active ingredient Fluorad >1 surfactant - a wetting agent to make the solution wet the bone more easily Sodium citrate >1 emulsifier , buffer Polyvinyl pyrrolidone >1 emulsifier They say less than one percent of the last three, but you really have no idea whether that is 1%, .1% or .01%. It could be any of those. But all those surfactants and emulsifiers are meant to keep the solution viable for long periods on the shelf. When you make it fresh you don't really need them. You can either buy a different decalcifier, or make your own. Making your own with just the water and acid will work just fine. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: M.O. via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, July 22, 2015 1:24 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] understanding reagents in decalcifier; making it in-house Hello Histonet, The supplier for our decalcifier, TBD-2 from Shandon, is having issues with getting the product out and we will not be receiving it for at least another month. Our samples are piling up and I don't know what I should do, but maybe I can make the decalcifier in-house. I am wondering if I can make my own based on the reagents they listed and their percentages and if certain reagents are not actually necessary. The samples we typically decalcify are mouse knees (decal time = 2 days), mouse spines (3 days), human bone slabs about 7mm in thickness (7-12 days). Fixation is in zinc buffered formalin, then decalcification, then 70% EtOH. Our choice to use TBD-2 is due to the gentle decalcification for IHC and we get GREAT results. Composition of Shandon TBD-2 Decalcifier: Component Weight % Water 77-80 Formic acid 21-23 Fluorad >1 Sodium citrate >1 Polyvinyl pyrrolidone >1 If you have any input on what reagents I should use and the percentages for making a decalcifier myself, it would be much appreciated! Thank you for you help, Merissa _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tjfinney2010 at gmail.com Wed Jul 22 16:52:22 2015 From: tjfinney2010 at gmail.com (=?utf-8?B?dGpmaW5uZXkyMDEwQGdtYWlsLmNvbQ==?=) Date: Wed, 22 Jul 2015 14:52:22 -0700 (PDT) Subject: [Histonet] Histotech Job Opening in Aurora 4 days/wk 10hr Shifts Message-ID: <000f425a.4a0a16ce2e8b719f@gmail.com> Were at Happy Connecting. Sent from my Sprint Phone. ------ Original message------ From: Melissa Owens via Histonet Date: Wed, Jul 22, 2015 2:21 PM To: histonet at lists.utsouthwestern.edu; Subject:[Histonet] Histotech Job Opening in Aurora 4 days/wk 10hr Shifts Hello, I have a permanent position for a histotech interested in a Monday-Thursday 10 hour shifts position. Must be ASCP certified with 2+ years of experience. Please email or call me for more details. Melissa Owens (Phelan) President, Laboratory Staffing Allied Search Partners www.linkedin.com/in/melissaphelan/ http://www.alliedsearchpartners.com T: 888.388.7571 ext. 102 F: 888.388.7572 *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with "remove." This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght at yahoo.com Wed Jul 22 21:30:02 2015 From: tkngflght at yahoo.com (Cheryl) Date: Wed, 22 Jul 2015 19:30:02 -0700 Subject: [Histonet] Austin for the summer? Message-ID: Need a day shift temp. Monday through Friday starting August 3 or 10. 13+ weeks. Holler! Cheryl 281.883.7704 Please excuse typos-sent from a phone. From areichard at lmhealth.org Thu Jul 23 06:14:16 2015 From: areichard at lmhealth.org (Amanda Reichard) Date: Thu, 23 Jul 2015 07:14:16 -0400 Subject: [Histonet] Gemini AS Stainer Message-ID: Good Morning Histonet! I've looked through the archives and couldn't find anything recent so I thought I would just go ahead and ask... We currently have a Prisma that we love for H&E's but we are looking for a cytology stainer. Possibly one that would take up less counter space. Has anyone had experience with the Thermo Gemini AS stainer? If so what are the pro's/cons's/love/hates? Thanks in advance! Amanda Reichard, HTL (ASCP)cm Histology Supervisor Laboratory Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (740) 348-4163 ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From richard.wild at wanadoo.fr Thu Jul 23 09:20:35 2015 From: richard.wild at wanadoo.fr (richard wild) Date: Thu, 23 Jul 2015 16:20:35 +0200 Subject: [Histonet] foot pedal / microm hm355s microtome Message-ID: <55B0F833.6060902@wanadoo.fr> Hi In the manual of the microm hm355s automated microtome: It is written "..._always connect the foot pedal... _" ... otherwise the brake is activated... Is it possible to disconnect that brake without the foot pedal and fully use the microtome without that pedal ? (there is a five lead connector, so may be linking some connectors with a simple wire could be ok ? Does anyone know about that connector ?) Other solution: does anyone have a spare Microm foot pedal ? Best regards Richard From mbireir at yahoo.com Thu Jul 23 09:41:31 2015 From: mbireir at yahoo.com (Manahil) Date: Thu, 23 Jul 2015 18:41:31 +0400 Subject: [Histonet] Processing and H&E Validation Message-ID: Does anyone have processing and H&E auto stainer machines validation procedure they would like to share? If our lab would like to change the bluing and differentiation reagent from in house to commercial, do we need to revalidate the stain? Thanks and appreciate your input. Manahil Sent from my iPhone From cjohnson at nmda.nmsu.edu Thu Jul 23 16:13:53 2015 From: cjohnson at nmda.nmsu.edu (Johnson, Carole) Date: Thu, 23 Jul 2015 21:13:53 +0000 Subject: [Histonet] Vet lab accreditation Message-ID: Hello and Happy Almost Friday, This question is for the veterinary labs: Who is your accrediting body? Is it AAVLD, NAHLN and/or are you CLI, CAP, AABB, JCI ISO 15189 or Joint Commission? I appreciate your input. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From esulkosky at gmail.com Thu Jul 23 17:23:19 2015 From: esulkosky at gmail.com (Eric Sulkosky) Date: Thu, 23 Jul 2015 18:23:19 -0400 Subject: [Histonet] Histology Opportunity in Rockford, Illinois Message-ID: <9EF0E541-85A6-4792-B9B3-69FA0347D25F@gmail.com> New Histology lab opening soon in Rockford, Illinois. They are recruiting for a fulltime histotechnician to accession, gross, process, embed, cut and stain GI biopsies. ESSENTIAL JOB FUNCTIONS: Knowledge: Demonstrate proficiency with accessioning, grossing, microtomes, tissue processors, stainers, and equipment used to produce a stained and labeled slide Perform microscopic evaluation of own work and uses the information gained to reduce error and improve performance. Can recognize positive results. Can differentiate incorrectly performed work and correct it. Reviews all procedure manuals, safety manuals, equipment operator's manuals and current policy Demonstrate complete understanding of all test procedures. Possess leadership and interpersonal skills commensurate with assigned responsibilities. Learns specialty areas of test performance. Physical and Cognitive Requirements: Ability to master delicate mechanical movements carefully and safely perform tasks requiring incremental adjustments. Ability to physically, audibly, and visually (including color discrimination) perform all functions; with or without reasonable accommodation. Education: A.S. Degree in Histology required, B.S. Degree desired. Experience: H.T. and/or H.T.L. (ASCP), or equivalent experience and training required. Previous experience as a technician or technologist is preferred. Two years of experience at an acute care hospital of more than 200 beds, or a reference lab is desired. Interested candidates should forward resumes to: NGarry at rockfordgi.com Please NO phone calls. From sheryl.stephenson at ag.state.nj.us Fri Jul 24 07:39:19 2015 From: sheryl.stephenson at ag.state.nj.us (Stephenson, Sheryl) Date: Fri, 24 Jul 2015 12:39:19 +0000 Subject: [Histonet] Vet lab accreditation In-Reply-To: References: Message-ID: NAHLN Sheryl Stephenson Histotechnologist NJ Department of Agriculture Animal Health Diagnostic Labs -----Original Message----- From: Johnson, Carole via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 23, 2015 5:14 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Vet lab accreditation Hello and Happy Almost Friday, This question is for the veterinary labs: Who is your accrediting body? Is it AAVLD, NAHLN and/or are you CLI, CAP, AABB, JCI ISO 15189 or Joint Commission? I appreciate your input. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mkent at dermpathlab.com Fri Jul 24 08:44:41 2015 From: mkent at dermpathlab.com (Michael Kent) Date: Fri, 24 Jul 2015 09:44:41 -0400 Subject: [Histonet] Film Coverslip Message-ID: Good morning, We are considering changing coverslip film for our high volume Sakura Prisma with coverslipper. We have tested StatLab film and pathologists are fine, and have not seen media build up on the instrument. Sakura is cautioning against alternatives for lamination and media build-up issues. Any feedback from Histonetters will be appreciated. Best and have a great weekend, Mike From rjbuesa at yahoo.com Fri Jul 24 08:50:14 2015 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 24 Jul 2015 13:50:14 +0000 (UTC) Subject: [Histonet] Film Coverslip In-Reply-To: References: Message-ID: <1538854966.1691632.1437745814815.JavaMail.yahoo@mail.yahoo.com> If you are using the Sakura instrument, please do not use other film than Sakura's. It is not only much better but also will allow the coverslipper to work better.Sometimes a cheaper option will be more costly at the end.Ren? On Friday, July 24, 2015 9:47 AM, Michael Kent via Histonet wrote: Good morning, We are considering changing coverslip film for our high volume Sakura Prisma with coverslipper.? We have tested StatLab film and pathologists are fine, and have not seen media build up on the instrument. Sakura is cautioning against alternatives for lamination and media build-up issues.? Any feedback from Histonetters will be appreciated. Best and have a great weekend, Mike _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ronald.Houston at nationwidechildrens.org Fri Jul 24 09:21:20 2015 From: Ronald.Houston at nationwidechildrens.org (Houston, Ronald) Date: Fri, 24 Jul 2015 14:21:20 +0000 Subject: [Histonet] Film Coverslip In-Reply-To: <1538854966.1691632.1437745814815.JavaMail.yahoo@mail.yahoo.com> References: <1538854966.1691632.1437745814815.JavaMail.yahoo@mail.yahoo.com> Message-ID: At previous place of employment we used Statlab's tape - absolutely no problems with stain bleeding or fading, bubbles or peeling of the tape. There were no problems with the coverslipper either. Of course Sakura is going to warn against using any other product but theirs. Consumables are how they stay in business. Ronnie -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, July 24, 2015 9:50 AM To: Michael Kent; Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Film Coverslip If you are using the Sakura instrument, please do not use other film than Sakura's. It is not only much better but also will allow the coverslipper to work better.Sometimes a cheaper option will be more costly at the end.Ren? On Friday, July 24, 2015 9:47 AM, Michael Kent via Histonet wrote: Good morning, We are considering changing coverslip film for our high volume Sakura Prisma with coverslipper.? We have tested StatLab film and pathologists are fine, and have not seen media build up on the instrument. Sakura is cautioning against alternatives for lamination and media build-up issues.? Any feedback from Histonetters will be appreciated. Best and have a great weekend, Mike _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQIGaQ&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=RufHkqFubCpo3-LgDyoM-j2btPBF8TK8U91KmVVuTQ4&s=rDlVheZRVLr9DBjR0VW0ejS5nf827G5F3gzCYzHTdqg&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQIGaQ&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=RufHkqFubCpo3-LgDyoM-j2btPBF8TK8U91KmVVuTQ4&s=rDlVheZRVLr9DBjR0VW0ejS5nf827G5F3gzCYzHTdqg&e= From cfields at mlkch.org Fri Jul 24 10:03:51 2015 From: cfields at mlkch.org (Carol Fields) Date: Fri, 24 Jul 2015 15:03:51 +0000 Subject: [Histonet] Film Coverslip In-Reply-To: References: <1538854966.1691632.1437745814815.JavaMail.yahoo@mail.yahoo.com> Message-ID: We used Mercedes tape and it worked ok. It is thicker and one pathologist preferred it. The drawback was you had to keep the Coverslipper clean or it would get sticky and cause problems. We had no staining issues. Have a great weekend. Carole -----Original Message----- From: Houston, Ronald via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, July 24, 2015 7:21 AM To: Rene J Buesa; Michael Kent Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Film Coverslip At previous place of employment we used Statlab's tape - absolutely no problems with stain bleeding or fading, bubbles or peeling of the tape. There were no problems with the coverslipper either. Of course Sakura is going to warn against using any other product but theirs. Consumables are how they stay in business. Ronnie -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, July 24, 2015 9:50 AM To: Michael Kent; Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Film Coverslip If you are using the Sakura instrument, please do not use other film than Sakura's. It is not only much better but also will allow the coverslipper to work better.Sometimes a cheaper option will be more costly at the end.Ren? On Friday, July 24, 2015 9:47 AM, Michael Kent via Histonet wrote: Good morning, We are considering changing coverslip film for our high volume Sakura Prisma with coverslipper.? We have tested StatLab film and pathologists are fine, and have not seen media build up on the instrument. Sakura is cautioning against alternatives for lamination and media build-up issues.? Any feedback from Histonetters will be appreciated. Best and have a great weekend, Mike _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQIGaQ&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=RufHkqFubCpo3-LgDyoM-j2btPBF8TK8U91KmVVuTQ4&s=rDlVheZRVLr9DBjR0VW0ejS5nf827G5F3gzCYzHTdqg&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQIGaQ&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=RufHkqFubCpo3-LgDyoM-j2btPBF8TK8U91KmVVuTQ4&s=rDlVheZRVLr9DBjR0VW0ejS5nf827G5F3gzCYzHTdqg&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kiran_g at sbcglobal.net Fri Jul 24 10:44:34 2015 From: kiran_g at sbcglobal.net (Kiran) Date: Fri, 24 Jul 2015 08:44:34 -0700 Subject: [Histonet] Dako Artisan special stainer In-Reply-To: References: Message-ID: <690C0E00-7A18-4018-99E5-F41BF80FE019@sbcglobal.net> Dear all, We are looking at another vendor for special stains. Any input or feedback on Dako special stainer will be much appreciated. Thanks and have great weekend. Kiran > On Jul 23, 2015, at 7:41 AM, Manahil via Histonet wrote: > > Does anyone have processing and H&E auto stainer machines validation procedure they would like to share? > If our lab would like to change the bluing and differentiation reagent from in house to commercial, do we need to revalidate the stain? > Thanks and appreciate your input. > Manahil > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 at cdc.gov Fri Jul 24 11:05:52 2015 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Fri, 24 Jul 2015 16:05:52 +0000 Subject: [Histonet] Dako Artisan special stainer In-Reply-To: <690C0E00-7A18-4018-99E5-F41BF80FE019@sbcglobal.net> References: , <690C0E00-7A18-4018-99E5-F41BF80FE019@sbcglobal.net> Message-ID: <3B2CD438E1628A41BD687E98B963B78137F8202F@EMBX-CLFT4.cdc.gov> We love our Artisan! We use it for Warthin-Starry, Gram, AFB and GMS staining. There are many more stains for the Artisan...these are just the ones we use. Jeanine Sanders CDC Atlanta ________________________________________ From: Kiran via Histonet [histonet at lists.utsouthwestern.edu] Sent: Friday, July 24, 2015 11:44 AM To: Manahil Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Dako Artisan special stainer Dear all, We are looking at another vendor for special stains. Any input or feedback on Dako special stainer will be much appreciated. Thanks and have great weekend. Kiran > On Jul 23, 2015, at 7:41 AM, Manahil via Histonet wrote: > > Does anyone have processing and H&E auto stainer machines validation procedure they would like to share? > If our lab would like to change the bluing and differentiation reagent from in house to commercial, do we need to revalidate the stain? > Thanks and appreciate your input. > Manahil > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christen at vet.k-state.edu Fri Jul 24 11:09:03 2015 From: christen at vet.k-state.edu (Shelly Christenson) Date: Fri, 24 Jul 2015 16:09:03 +0000 Subject: [Histonet] Vet lab accreditation In-Reply-To: References: Message-ID: AAVLD Shelly Christenson HT (ASCP) Veterinary Diagnostic Lab Histopathology Mosier Hall L-216 Kansas State University 1800 Denison Manhattan, KS 66506 785/532-4464 christen at vet.k-state.edu -----Original Message----- From: Johnson, Carole via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 23, 2015 4:14 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Vet lab accreditation Hello and Happy Almost Friday, This question is for the veterinary labs: Who is your accrediting body? Is it AAVLD, NAHLN and/or are you CLI, CAP, AABB, JCI ISO 15189 or Joint Commission? I appreciate your input. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JRobinson at pathology-associates.com Fri Jul 24 11:21:35 2015 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Fri, 24 Jul 2015 16:21:35 +0000 Subject: [Histonet] Film Coverslip In-Reply-To: References: <1538854966.1691632.1437745814815.JavaMail.yahoo@mail.yahoo.com> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D9E8B0@PAEXCH1.PathologyAssociates.local> We used an alternative tape (I believe it was from Mercedes) years ago and we had some major issues with the tape detaching from the slide over time (in storage) and taking the tissue on the slide with it. Perhaps the xylene being dispensed needed to be adjusted or some other technical problem was in play (such as storage conditions) but we did not feel it was worth the risk. We went back to the Sakura tape and as far as I know we have not had any more problems with detaching tape. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Carol Fields via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, July 24, 2015 8:04 AM To: Houston, Ronald Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Film Coverslip We used Mercedes tape and it worked ok. It is thicker and one pathologist preferred it. The drawback was you had to keep the Coverslipper clean or it would get sticky and cause problems. We had no staining issues. Have a great weekend. Carole -----Original Message----- From: Houston, Ronald via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, July 24, 2015 7:21 AM To: Rene J Buesa; Michael Kent Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Film Coverslip At previous place of employment we used Statlab's tape - absolutely no problems with stain bleeding or fading, bubbles or peeling of the tape. There were no problems with the coverslipper either. Of course Sakura is going to warn against using any other product but theirs. Consumables are how they stay in business. Ronnie -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, July 24, 2015 9:50 AM To: Michael Kent; Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Film Coverslip If you are using the Sakura instrument, please do not use other film than Sakura's. It is not only much better but also will allow the coverslipper to work better.Sometimes a cheaper option will be more costly at the end.Ren? On Friday, July 24, 2015 9:47 AM, Michael Kent via Histonet wrote: Good morning, We are considering changing coverslip film for our high volume Sakura Prisma with coverslipper. We have tested StatLab film and pathologists are fine, and have not seen media build up on the instrument. Sakura is cautioning against alternatives for lamination and media build-up issues. Any feedback from Histonetters will be appreciated. Best and have a great weekend, Mike _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQIGaQ&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=RufHkqFubCpo3-LgDyoM-j2btPBF8TK8U91KmVVuTQ4&s=rDlVheZRVLr9DBjR0VW0ejS5nf827G5F3gzCYzHTdqg&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=BQIGaQ&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=RufHkqFubCpo3-LgDyoM-j2btPBF8TK8U91KmVVuTQ4&s=rDlVheZRVLr9DBjR0VW0ejS5nf827G5F3gzCYzHTdqg&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From mjones at metropath.com Fri Jul 24 11:49:19 2015 From: mjones at metropath.com (Michael Ann Jones) Date: Fri, 24 Jul 2015 16:49:19 +0000 Subject: [Histonet] Film Coverslip In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D9E8B0@PAEXCH1.PathologyAssociates.local> References: <1538854966.1691632.1437745814815.JavaMail.yahoo@mail.yahoo.com> <204A03EB5A7F0A4BB1EEDD52A963829C16D9E8B0@PAEXCH1.PathologyAssociates.local> Message-ID: We also used different tapes with our Sakura cover slipper with not super quality results. We also have stuck with the Sakura tape (and it?s expensive!) Good luck! Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones at metropath.com On 7/24/15, 10:21 AM, "Jeffrey Robinson via Histonet" wrote: >We used an alternative tape (I believe it was from Mercedes) years ago >and we had some major issues with the tape detaching from the slide over >time (in storage) and taking the tissue on the slide with it. Perhaps >the xylene being dispensed needed to be adjusted or some other technical >problem was in play (such as storage conditions) but we did not feel it >was worth the risk. We went back to the Sakura tape and as far as I know >we have not had any more problems with detaching tape. >Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. > >-----Original Message----- >From: Carol Fields via Histonet [mailto:histonet at lists.utsouthwestern.edu] >Sent: Friday, July 24, 2015 8:04 AM >To: Houston, Ronald >Cc: histonet at lists.utsouthwestern.edu >Subject: Re: [Histonet] Film Coverslip > >We used Mercedes tape and it worked ok. It is thicker and one >pathologist preferred it. The drawback was you had to keep the >Coverslipper clean or it would get sticky and cause problems. >We had no staining issues. >Have a great weekend. >Carole > > >-----Original Message----- >From: Houston, Ronald via Histonet >[mailto:histonet at lists.utsouthwestern.edu] >Sent: Friday, July 24, 2015 7:21 AM >To: Rene J Buesa; Michael Kent >Cc: histonet at lists.utsouthwestern.edu >Subject: Re: [Histonet] Film Coverslip > >At previous place of employment we used Statlab's tape - absolutely no >problems with stain bleeding or fading, bubbles or peeling of the tape. >There were no problems with the coverslipper either. > >Of course Sakura is going to warn against using any other product but >theirs. Consumables are how they stay in business. >Ronnie > >-----Original Message----- >From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] >Sent: Friday, July 24, 2015 9:50 AM >To: Michael Kent; Histonet at lists.utsouthwestern.edu >Subject: Re: [Histonet] Film Coverslip > >If you are using the Sakura instrument, please do not use other film than >Sakura's. It is not only much better but also will allow the coverslipper >to work better.Sometimes a cheaper option will be more costly at the >end.Ren? > > > On Friday, July 24, 2015 9:47 AM, Michael Kent via Histonet > wrote: > > > Good morning, We are considering changing coverslip film for our high >volume Sakura Prisma with coverslipper. We have tested StatLab film and >pathologists are fine, and have not seen media build up on the instrument. >Sakura is cautioning against alternatives for lamination and media >build-up issues. Any feedback from Histonetters will be appreciated. >Best and have a great weekend, >Mike >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.e >du_mailman_listinfo_histonet&d=BQIGaQ&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__ggl >PkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3o >yZ&m=RufHkqFubCpo3-LgDyoM-j2btPBF8TK8U91KmVVuTQ4&s=rDlVheZRVLr9DBjR0VW0ejS >5nf827G5F3gzCYzHTdqg&e= > > > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.e >du_mailman_listinfo_histonet&d=BQIGaQ&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__ggl >PkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3o >yZ&m=RufHkqFubCpo3-LgDyoM-j2btPBF8TK8U91KmVVuTQ4&s=rDlVheZRVLr9DBjR0VW0ejS >5nf827G5F3gzCYzHTdqg&e= >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >This email and attachments may contain PHI that is privileged and >confidential and is not intended for any unauthorized person. If you, the >reader, are not the intended recipient you are hereby notified that any >dissemination, distribution or copying of this communication is strictly >prohibited. Do not read the email but instead reply to the sender and >destroy the message and any attachments. Thank you. >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sun at pathology.ufl.edu Fri Jul 24 12:20:08 2015 From: sun at pathology.ufl.edu (Sun,Yuping) Date: Fri, 24 Jul 2015 17:20:08 +0000 Subject: [Histonet] Hi Message-ID: <9ad9546352b14add8111cebcda2fb0b8@AHC-EXCH04.ad.ufl.edu> test From jclark at pcnm.com Fri Jul 24 12:47:02 2015 From: jclark at pcnm.com (Joanne Clark) Date: Fri, 24 Jul 2015 17:47:02 +0000 Subject: [Histonet] Assistant Supervisor Job Available Message-ID: <0494A7D4E8CC254EA2FB81464982E378D9AB1EC0@S10MAILD001N3.SH10.lan> Hi All, we have a position available for an Assistant Supervisor in Roswell, located in sunny southeast New Mexico. All routine histology skills will be needed (processing, embedding, cutting, special stains, frozen sections, etc.). They would be responsible for the smooth running of the histology lab in my absence and would rotate with me on immunohistochemistry and grossing bench. The successful candidate will need to have a minimum of an Associate's Degree in Science (to be able to gross) and ASCP certification. Grossing experience would be a definite asset as would supervisory experience, but we would be willing to train the right candidate. Joanne Clark, BAAS, HT Director of Histology Pathology Consultants of New Mexico Roswell, New Mexico 575-622-5600 x 221 Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From TNMayer at mdanderson.org Fri Jul 24 12:58:22 2015 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Fri, 24 Jul 2015 17:58:22 +0000 Subject: [Histonet] ] Dako Artisan special stainer Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC88224E9F67@D1PWPEXMBX05.mdanderson.edu> I have used the older Dako Artisan and loved it. It is easy to adapt to you lab protocols and if it goes down, you can easily pick up from where you left off. The customer service was great (it has been a while since I had an instrument), and replacement kits and labels were easy to get. The stains are easy to read and the pathologists like them. Warthin Starry used to be only 7 min and very clean. Multiple stains can be performed at once, including AFB and GMS. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 Message: 8 Date: Fri, 24 Jul 2015 08:44:34 -0700 From: Kiran To: Manahil Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Dako Artisan special stainer Message-ID: <690C0E00-7A18-4018-99E5-F41BF80FE019 at sbcglobal.net> Content-Type: text/plain; charset=us-ascii Dear all, We are looking at another vendor for special stains. Any input or feedback on Dako special stainer will be much appreciated. Thanks and have great weekend. Kiran > On Jul 23, 2015, at 7:41 AM, Manahil via Histonet wrote: > > Does anyone have processing and H&E auto stainer machines validation procedure they would like to share? > If our lab would like to change the bluing and differentiation reagent from in house to commercial, do we need to revalidate the stain? > Thanks and appreciate your input. > Manahil > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 24 Jul 2015 16:05:52 +0000 From: "Sanders, Jeanine (CDC/OID/NCEZID)" To: Kiran , Manahil Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Dako Artisan special stainer Message-ID: <3B2CD438E1628A41BD687E98B963B78137F8202F at EMBX-CLFT4.cdc.gov> Content-Type: text/plain; charset="us-ascii" We love our Artisan! We use it for Warthin-Starry, Gram, AFB and GMS staining. There are many more stains for the Artisan...these are just the ones we use. Jeanine Sanders CDC Atlanta From gayle.callis at bresnan.net Sat Jul 25 12:24:19 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Sat, 25 Jul 2015 11:24:19 -0600 Subject: [Histonet] Re. Decalcification with formic acid sodium citrate Message-ID: <000001d0c6fe$bddcc760$39965620$@bresnan.net> Merissa and Tim, This formic acid decalcifying solution is basically the classic Evans and Krajian fluid (Sheehan and Hrapchak, Theory and Practice of Histotechnology, 2nd edition, P.92). Shandon has added other ingredients for some reason, and has kept those concentrations proprietary. You really don't need to add a surfactant or PVP emulsifier when making up this decalcifying agent. Simply use the classic recipe for successful decalcification. This is also referred to as buffered formic acid and in some publications an "acidic buffer". It is excellent if IHC is needed and less damaging, obviously, than a strong mineral HCL acid decalcifiers. Sodium citrate crystals (a buffering salt) 10 g 90% formic acid stock 25 ml Distilled water 75 ml One can calculate the concentration of formic acid i.e. approx. 4.5% since is it made from 90% formic acid stock. Don't bother with the surfactants or PVP. Enjoy an excellent in house formic acid decalcifying solution. I also suggest you read Sheehan and Hrapchak textbook chapter on bone as a way to familiarize yourself with decalcifiying solutions that manufacturers now supply with some modifications. Some manufacturers will refer to these methods but probably prefer not to do this since they want you to buy their commercial product that is obviously a time saver with elimination of having to store stock acid solutions. The classic methods made in house are excellent if you have time to make them up. Formic acid with sodium formate is another popular buffered formic acid. I suggest you look for another source/manufacturer of the your favorite decalcifier in question as more than one company will make it. Decal Corp, recently sold to Stat Lab, could also be the source as Shandon isn't the only game in town. Others are Newcomer Supply, Poly Scientific. Not having to make it up may remain your preference. Gayle M. Callis HTL/HT/MT(ASCP) Written by Tim and Merissa: Merissa, Water 77-80 solvent Formic acid 21-23 active ingredient Fluorad >1 surfactant - a wetting agent to make the solution wet the bone more easily Sodium citrate >1 emulsifier , buffer Polyvinyl pyrrolidone >1 emulsifier They say less than one percent of the last three, but you really have no idea whether that is 1%, .1% or .01%. It could be any of those. But all those surfactants and emulsifiers are meant to keep the solution viable for long periods on the shelf. When you make it fresh you don't really need them. You can either buy a different decalcifier, or make your own. Making your own with just the water and acid will work just fine. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: M.O. via Histonet [mailto: histonet at lists.utsouthwestern.edu] Sent: Wednesday, July 22, 2015 1:24 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] understanding reagents in decalcifier; making it in-house Hello Histonet The supplier for our decalcifier, TBD-2 from Shandon, is having issues with getting the product out and we will not be receiving it for at least another month. Our samples are piling up and I don't know what I should do, but maybe I can make the decalcifier in-house. I am wondering if I can make my own based on the reagents they listed and their percentages and if certain reagents are not actually necessary. The samples we typically decalcify are mouse knees (decal time = 2 days), mouse spines (3 days), human bone slabs about 7mm in thickness (7-12 days). Fixation is in zinc buffered formalin, then decalcification, then 70% EtOH. Our choice to use TBD-2 is due to the gentle decalcification for IHC and we get GREAT results. Composition of Shandon TBD-2 Decalcifier: Component Weight % Water 77-80 Formic acid 21-23 Fluorad >1 Sodium citrate >1 Polyvinyl pyrrolidone >1 If you have any input on what reagents I should use and the percentages for making a decalcifier myself, it would be much appreciated! Thank you for you help, Merissa From modz9636 at gmail.com Sat Jul 25 12:54:59 2015 From: modz9636 at gmail.com (Merissa) Date: Sat, 25 Jul 2015 10:54:59 -0700 Subject: [Histonet] Re. Decalcification with formic acid sodium citrate In-Reply-To: <000001d0c6fe$bddcc760$39965620$@bresnan.net> References: <000001d0c6fe$bddcc760$39965620$@bresnan.net> Message-ID: <7E946DAD-9E26-43CB-B822-31D180C8CB71@gmail.com> Thank you, Gayle! This is exactly what I was looking for and we are willing to make this in house. We are trying just for if acid and water, but the buffering salt should be added. I will try de calcifying mouse knees next week with this protocol. Thank you for the reference, I appreciate your help! Sincerely, Merissa > On Jul 25, 2015, at 10:24 AM, Gayle Callis via Histonet wrote: > > Merissa and Tim, > > > > This formic acid decalcifying solution is basically the classic Evans and > Krajian fluid (Sheehan and Hrapchak, Theory and Practice of > Histotechnology, 2nd edition, P.92). Shandon has added other ingredients > for some reason, and has kept those concentrations proprietary. You really > don't need to add a surfactant or PVP emulsifier when making up this > decalcifying agent. Simply use the classic recipe for successful > decalcification. This is also referred to as buffered formic acid and in > some publications an "acidic buffer". It is excellent if IHC is needed and > less damaging, obviously, than a strong mineral HCL acid decalcifiers. > > > > Sodium citrate crystals (a buffering salt) 10 g > > 90% formic acid stock 25 ml > > Distilled water 75 ml > > > > One can calculate the concentration of formic acid i.e. approx. 4.5% since > is it made from 90% formic acid stock. > > > > Don't bother with the surfactants or PVP. > > > > Enjoy an excellent in house formic acid decalcifying solution. I also > suggest you read Sheehan and Hrapchak textbook chapter on bone as a way to > familiarize yourself with decalcifiying solutions that manufacturers now > supply with some modifications. Some manufacturers will refer to these > methods but probably prefer not to do this since they want you to buy their > commercial product that is obviously a time saver with elimination of having > to store stock acid solutions. The classic methods made in house are > excellent if you have time to make them up. Formic acid with sodium > formate is another popular buffered formic acid. I suggest you look for > another source/manufacturer of the your favorite decalcifier in question as > more than one company will make it. Decal Corp, recently sold to Stat Lab, > could also be the source as Shandon isn't the only game in town. Others > are Newcomer Supply, Poly Scientific. Not having to make it up may remain > your preference. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > > > > > > > > > > > > > > > Written by Tim and Merissa: > > > > Merissa, > > > > Water 77-80 solvent > > Formic acid 21-23 active ingredient > > Fluorad >1 surfactant - a > wetting agent to make the solution wet the bone more easily > > Sodium citrate >1 emulsifier , buffer > > Polyvinyl pyrrolidone >1 emulsifier > > > > They say less than one percent of the last three, but you really have no > idea whether that is 1%, .1% or .01%. It could be any of those. > > > > But all those surfactants and emulsifiers are meant to keep the solution > viable for long periods on the shelf. When you make it fresh you don't > really need them. > > > > You can either buy a different decalcifier, or make your own. Making your > own with just the water and acid will work just fine. > > > > > > Tim Morken > > Pathology Site Manager, Parnassus > > Supervisor, Electron Microscopy/Neuromuscular Special Studies > > Department of Pathology > > UC San Francisco Medical Center > > > > -----Original Message----- > > From: M.O. via Histonet [mailto: > histonet at > lists.utsouthwestern.edu] > > Sent: Wednesday, July 22, 2015 1:24 PM > > To: histonet at > lists.utsouthwestern.edu > > Subject: [Histonet] understanding reagents in decalcifier; making it > in-house > > > > Hello Histonet > > > > The supplier for our decalcifier, TBD-2 from Shandon, is having issues with > getting the product out and we will not be receiving it for at least another > month. Our samples are piling up and I don't know what I should do, but > maybe I can make the decalcifier in-house. I am wondering if I can make my > own based on the reagents they listed and their percentages and if certain > reagents are not actually necessary. > > > > The samples we typically decalcify are mouse knees (decal time = 2 days), > mouse spines (3 days), human bone slabs about 7mm in thickness (7-12 days). > Fixation is in zinc buffered formalin, then decalcification, then 70% EtOH. > Our choice to use TBD-2 is due to the gentle decalcification for IHC and we > get GREAT results. > > > > Composition of Shandon TBD-2 Decalcifier: > > Component Weight % > > Water 77-80 > > Formic acid 21-23 > > Fluorad >1 > > Sodium citrate >1 > > Polyvinyl pyrrolidone >1 > > > > If you have any input on what reagents I should use and the percentages for > making a decalcifier myself, it would be much appreciated! > > > > Thank you for you help, > > Merissa > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abtdhu at gmail.com Sun Jul 26 13:33:47 2015 From: abtdhu at gmail.com (abtdhu at gmail.com) Date: Sun, 26 Jul 2015 14:33:47 -0400 Subject: [Histonet] Decalcification with formic acid sodium citrate In-Reply-To: References: Message-ID: <3950524D-9471-4473-8FFC-FD897B3D775C@gmail.com> There was a paper http://www.genedetect.com/Merchant2/ExampleRefs/Decalcifying_protocols.pdf Talking about formic acid (Morse solution) can get as good result as EDTA in ISH. FYI. Dorothy Hu > Today's Topics: > > 1. Re. Decalcification with formic acid sodium citrate > (Gayle Callis) > 2. Re: Re. Decalcification with formic acid > > Merissa and Tim, > > > > This formic acid decalcifying solution is basically the classic Evans and > Krajian fluid (Sheehan and Hrapchak, Theory and Practice of > Histotechnology, 2nd edition, P.92). Shandon has added other ingredients > for some reason, and has kept those concentrations proprietary. You really > don't need to add a surfactant or PVP emulsifier when making up this > decalcifying agent. Simply use the classic recipe for successful > decalcification. This is also referred to as buffered formic acid and in > some publications an "acidic buffer". It is excellent if IHC is needed and > less damaging, obviously, than a strong mineral HCL acid decalcifiers. > > > > Sodium citrate crystals (a buffering salt) 10 g > > 90% formic acid stock 25 ml > > Distilled water 75 ml > > > > One can calculate the concentration of formic acid i.e. approx. 4.5% since > is it made from 90% formic acid stock. > > > > Don't bother with the surfactants or PVP. > > > > Enjoy an excellent in house formic acid decalcifying solution. I also > suggest you read Sheehan and Hrapchak textbook chapter on bone as a way to > familiarize yourself with decalcifiying solutions that manufacturers now > supply with some modifications. Some manufacturers will refer to these > methods but probably prefer not to do this since they want you to buy their > commercial product that is obviously a time saver with elimination of having > to store stock acid solutions. The classic methods made in house are > excellent if you have time to make them up. Formic acid with sodium > formate is another popular buffered formic acid. I suggest you look for > another source/manufacturer of the your favorite decalcifier in question as > more than one company will make it. Decal Corp, recently sold to Stat Lab, > could also be the source as Shandon isn't the only game in town. Others > are Newcomer Supply, Poly Scientific. Not having to make it up may remain > your preference. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > > > > > > > > > > > > > > > > > Written by Tim and Merissa: > > > > Merissa, > > > > Water 77-80 solvent > > Formic acid 21-23 active ingredient > > Fluorad >1 surfactant - a > wetting agent to make the solution wet the bone more easily > > Sodium citrate >1 emulsifier , buffer > > Polyvinyl pyrrolidone >1 emulsifier > > > > They say less than one percent of the last three, but you really have no > idea whether that is 1%, .1% or .01%. It could be any of those. > > > > But all those surfactants and emulsifiers are meant to keep the solution > viable for long periods on the shelf. When you make it fresh you don't > really need them. > > > > You can either buy a different decalcifier, or make your own. Making your > own with just the water and acid will work just fine. > > > > > > Tim Morken > > Pathology Site Manager, Parnassus > > Supervisor, Electron Microscopy/Neuromuscular Special Studies > > Department of Pathology > > UC San Francisco Medical Center > > > > -----Original Message----- > > From: M.O. via Histonet [mailto: > histonet at > lists.utsouthwestern.edu] > > Sent: Wednesday, July 22, 2015 1:24 PM > > To: histonet at > lists.utsouthwestern.edu > > Subject: [Histonet] understanding reagents in decalcifier; making it > in-house > > > > Hello Histonet > > > > The supplier for our decalcifier, TBD-2 from Shandon, is having issues with > getting the product out and we will not be receiving it for at least another > month. Our samples are piling up and I don't know what I should do, but > maybe I can make the decalcifier in-house. I am wondering if I can make my > own based on the reagents they listed and their percentages and if certain > reagents are not actually necessary. > > > > The samples we typically decalcify are mouse knees (decal time = 2 days), > mouse spines (3 days), human bone slabs about 7mm in thickness (7-12 days). > Fixation is in zinc buffered formalin, then decalcification, then 70% EtOH. > Our choice to use TBD-2 is due to the gentle decalcification for IHC and we > get GREAT results. > > > > Composition of Shandon TBD-2 Decalcifier: > > Component Weight % > > Water 77-80 > > Formic acid 21-23 > > Fluorad >1 > > Sodium citrate >1 > > Polyvinyl pyrrolidone >1 > > > > If you have any input on what reagents I should use and the percentages for > making a decalcifier myself, it would be much appreciated! > > > > Thank you for you help, > > Merissa > > > > > > ------------------------------ > > Message: 2 > Date: Sat, 25 Jul 2015 10:54:59 -0700 > From: Merissa > To: "gayle.callis at bresnan.net" > Cc: Histonet > Subject: Re: [Histonet] Re. Decalcification with formic acid sodium > citrate > Message-ID: <7E946DAD-9E26-43CB-B822-31D180C8CB71 at gmail.com> > Content-Type: text/plain; charset=us-ascii > > Thank you, Gayle! This is exactly what I was looking for and we are willing to make this in house. We are trying just for if acid and water, but the buffering salt should be added. I will try de calcifying mouse knees next week with this protocol. Thank you for the reference, I appreciate your help! > > Sincerely, > Merissa > > > >> On Jul 25, 2015, at 10:24 AM, Gayle Callis via Histonet wrote: >> >> Merissa and Tim, >> >> >> >> This formic acid decalcifying solution is basically the classic Evans and >> Krajian fluid (Sheehan and Hrapchak, Theory and Practice of >> Histotechnology, 2nd edition, P.92). Shandon has added other ingredients >> for some reason, and has kept those concentrations proprietary. You really >> don't need to add a surfactant or PVP emulsifier when making up this >> decalcifying agent. Simply use the classic recipe for successful >> decalcification. This is also referred to as buffered formic acid and in >> some publications an "acidic buffer". It is excellent if IHC is needed and >> less damaging, obviously, than a strong mineral HCL acid decalcifiers. >> >> >> >> Sodium citrate crystals (a buffering salt) 10 g >> >> 90% formic acid stock 25 ml >> >> Distilled water 75 ml >> >> >> >> One can calculate the concentration of formic acid i.e. approx. 4.5% since >> is it made from 90% formic acid stock. >> >> >> >> Don't bother with the surfactants or PVP. >> >> >> >> Enjoy an excellent in house formic acid decalcifying solution. I also >> suggest you read Sheehan and Hrapchak textbook chapter on bone as a way to >> familiarize yourself with decalcifiying solutions that manufacturers now >> supply with some modifications. Some manufacturers will refer to these >> methods but probably prefer not to do this since they want you to buy their >> commercial product that is obviously a time saver with elimination of having >> to store stock acid solutions. The classic methods made in house are >> excellent if you have time to make them up. Formic acid with sodium >> formate is another popular buffered formic acid. I suggest you look for >> another source/manufacturer of the your favorite decalcifier in question as >> more than one company will make it. Decal Corp, recently sold to Stat Lab, >> could also be the source as Shandon isn't the only game in town. Others >> are Newcomer Supply, Poly Scientific. Not having to make it up may remain >> your preference. >> >> >> >> Gayle M. Callis >> >> HTL/HT/MT(ASCP) >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> >> Written by Tim and Merissa: >> >> >> >> Merissa, >> >> >> >> Water 77-80 solvent >> >> Formic acid 21-23 active ingredient >> >> Fluorad >1 surfactant - a >> wetting agent to make the solution wet the bone more easily >> >> Sodium citrate >1 emulsifier , buffer >> >> Polyvinyl pyrrolidone >1 emulsifier >> >> >> >> They say less than one percent of the last three, but you really have no >> idea whether that is 1%, .1% or .01%. It could be any of those. >> >> >> >> But all those surfactants and emulsifiers are meant to keep the solution >> viable for long periods on the shelf. When you make it fresh you don't >> really need them. >> >> >> >> You can either buy a different decalcifier, or make your own. Making your >> own with just the water and acid will work just fine. >> >> >> >> >> >> Tim Morken >> >> Pathology Site Manager, Parnassus >> >> Supervisor, Electron Microscopy/Neuromuscular Special Studies >> >> Department of Pathology >> >> UC San Francisco Medical Center >> >> >> >> -----Original Message----- >> >> From: M.O. via Histonet [mailto: >> histonet at >> lists.utsouthwestern.edu] >> >> Sent: Wednesday, July 22, 2015 1:24 PM >> >> To: histonet at >> lists.utsouthwestern.edu >> >> Subject: [Histonet] understanding reagents in decalcifier; making it >> in-house >> >> >> >> Hello Histonet >> >> >> >> The supplier for our decalcifier, TBD-2 from Shandon, is having issues with >> getting the product out and we will not be receiving it for at least another >> month. Our samples are piling up and I don't know what I should do, but >> maybe I can make the decalcifier in-house. I am wondering if I can make my >> own based on the reagents they listed and their percentages and if certain >> reagents are not actually necessary. >> >> >> >> The samples we typically decalcify are mouse knees (decal time = 2 days), >> mouse spines (3 days), human bone slabs about 7mm in thickness (7-12 days). >> Fixation is in zinc buffered formalin, then decalcification, then 70% EtOH. >> Our choice to use TBD-2 is due to the gentle decalcification for IHC and we >> get GREAT results. >> >> >> >> Composition of Shandon TBD-2 Decalcifier: >> >> Component Weight % >> >> Water 77-80 >> >> Formic acid 21-23 >> >> Fluorad >1 >> >> Sodium citrate >1 >> >> Polyvinyl pyrrolidone >1 >> >> >> >> If you have any input on what reagents I should use and the percentages for >> making a decalcifier myself, it would be much appreciated! >> >> >> >> Thank you for you help, >> >> Merissa >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 140, Issue 28 > ***************************************** From carl.hobbs at kcl.ac.uk Sun Jul 26 13:38:33 2015 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sun, 26 Jul 2015 18:38:33 +0000 Subject: [Histonet] Re. Decalcification with formic acid sodium Message-ID: Mouse knee joints: done lots of decalcified FFPWS for assessment of articular cartilage degeneration models. See Histonet images for a Tol blue image. Decal in 10 % EDTA for 3 days on a rocker at RT. Sure....5days if you are worried. No difference in Immuno-reactivity, imho. If you want to use buffered Formic acid, use Formic acid; sodium formate. Use of citric acid with Formic acid does not make a buffer. It's just mixing two relatively mild acids..... However, I am sure that Prof Kiernan can further enlighten us. Respectfully, Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From tony.henwood at health.nsw.gov.au Sun Jul 26 18:31:46 2015 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun, 26 Jul 2015 23:31:46 +0000 Subject: [Histonet] Dako Artisan special stainer In-Reply-To: <690C0E00-7A18-4018-99E5-F41BF80FE019@sbcglobal.net> References: <690C0E00-7A18-4018-99E5-F41BF80FE019@sbcglobal.net> Message-ID: <6D6BD1DE8A5571489398B392A38A71570197644E5F@xmdb04.nch.kids> Oh dear, Are you considering sacking your Special Stains Tech? Only kidding. I wish I had the money to buy one myself. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Kiran via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Saturday, 25 July 2015 1:45 AM To: Manahil Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Dako Artisan special stainer Dear all, We are looking at another vendor for special stains. Any input or feedback on Dako special stainer will be much appreciated. Thanks and have great weekend. Kiran > On Jul 23, 2015, at 7:41 AM, Manahil via Histonet wrote: > > Does anyone have processing and H&E auto stainer machines validation procedure they would like to share? > If our lab would like to change the bluing and differentiation reagent from in house to commercial, do we need to revalidate the stain? > Thanks and appreciate your input. > Manahil > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From jpalmer at svi.edu.au Sun Jul 26 19:55:22 2015 From: jpalmer at svi.edu.au (Jason Palmer) Date: Mon, 27 Jul 2015 10:55:22 +1000 (EST) Subject: [Histonet] axolotl lymphatics In-Reply-To: <1267660652.2411.1437958174751.JavaMail.root@zstore.medstv.unimelb.edu.au> Message-ID: <983727148.2470.1437958522140.JavaMail.root@zstore.medstv.unimelb.edu.au> Hi all, I need to find an antibody that will label lymphatic endothelial cells in the axolotl. Does anybody have any experience or ideas? I have tried a couple of our anti-mouse and anti-human Abs for podoplanin and LYVE-1 but no cross-reactivity so far. I have no experience with staining of non-mammalian tissues - maybe an anti-frog Ab would cross react? Does anyone have experience with other amphibians? Thanks, Jason -- Jason Palmer Histology Laboratory Coordinator O'Brien Institute / St Vincent's Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4045 fax +61 3 9416 0926 email: jpalmer at svi.edu.au From KSimeone at leavittmgt.com Mon Jul 27 06:45:06 2015 From: KSimeone at leavittmgt.com (Delray Beach Pathology Kari Simeone) Date: Mon, 27 Jul 2015 11:45:06 +0000 Subject: [Histonet] FT NIGHT (evening shift) POSITION DELRAY BCH FL Message-ID: <43944B1DBAAC2846B7B9D626B5F1233C3EB54465@vm-email.leavittmgt.com> Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Dermatology Lab. This is a permanent full time NIGHT SHIFT (40 hours) position with benefits (medical/401k/vacation) and shift differential. THIS IS A DRUG FREE WORKPLACE. Background check, personality test and drug test will be necessary. Sorry, no relocation assistance provided. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengimann at leavittmgt.com if interested. *full time position Mon-Fri or Sun-Thurs 6PM-2:30AM *MUST be licensed as a FL HISTOTEHCNOLOGIST ONLY (will be working solo half of your shift) *MUST have at LEAST 2 years experience (dermatology preferred) Please DO NOT respond if no EXPERIENCE! *VERY proficient in embedding and microtomy *must be self motivated, reliable and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) *some IHC experience preferred Kari M Simeone 561.819.6517 fax ksimeone at leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From fpearsa at clemson.edu Mon Jul 27 11:56:47 2015 From: fpearsa at clemson.edu (Fran Pearsall) Date: Mon, 27 Jul 2015 12:56:47 -0400 Subject: [Histonet] Special Stain kits Vet lab ques? Message-ID: <55B662CF.4090009@clemson.edu> This is an SOP question. I have several older kits that I have made up in-house solutions to replenish certain stains that have been used up without having to order a whole new kit. (eg. decolorizer) How do word the Stain SOP to be put into practice? How often will I have to revise it officially until a new kit is purchased? If the stain is coming out correctly, will this pass ISO standards? Thanks for any help Fran From Jose.R.deGuzman at gunet.georgetown.edu Mon Jul 27 13:27:51 2015 From: Jose.R.deGuzman at gunet.georgetown.edu (deGuzman, Jose R) Date: Mon, 27 Jul 2015 14:27:51 -0400 Subject: [Histonet] Dako Artisan Special Stainer In-Reply-To: References: Message-ID: Hi all, We recently upgraded our old Artisan to the Artisan Link Pro. It does the depar. within the system itself. Set-up was easy, we just transferred our old protocols to the new units. Modifying protocols was easy too. Staining is consistent, tech support and their specialists are reliable and share information from what other users do. If I have kit problems, a replacement kit is shipped overnight to the lab. Kits come in 50 test or 100 test, our GMS 100 test kit usually lasts us 1 month and staining is consistent. We run multiple protocols per run and found that certain combinations can go together to save time such as: GMS, Reticulin and Jones for a 1 hour 20 minute stain and Trichrome, PAS to 1 hour, 40 minutes. Last month, we did 369 stains and 347 stains in May on this system. I print out usage reports by protocols and case lists including which unit performed the stain. A quick scan of the barcode lets me know the lot number and expiration date of the kit used. Hope this was helpful. Jose de Guzman MedStar Health is a not-for-profit, integrated healthcare delivery system, the largest in Maryland and the Washington, D.C., region. Nationally recognized for clinical quality in heart, orthopaedics, cancer and GI. IMPORTANT: This e-mail (including any attachments) may contain information that is private, confidential, or protected by attorney-client or other privilege. If you received this e-mail in error, please delete it from your system without copying it and notify sender by reply e-mail, so that our records can be corrected. Thank you. Help conserve valuable resources - only print this email if necessary. From duhrich at hotmail.com Mon Jul 27 15:34:06 2015 From: duhrich at hotmail.com (dianna uhrich) Date: Mon, 27 Jul 2015 20:34:06 +0000 Subject: [Histonet] (no subject) Message-ID: Hi Need some advise on tissue cassette printers. What is everyone using, and what problems does everyone have From khong at waxitinc.com Mon Jul 27 17:26:06 2015 From: khong at waxitinc.com (Kai Hong) Date: Mon, 27 Jul 2015 15:26:06 -0700 Subject: [Histonet] Toluidine blue stain for MMA Message-ID: <11fca7646af29705683356042da527cf@waxitinc.com> Hi, is there anyone have an experience with MMA toluidine staining? Im using T7200, T9100, Osteo-bed resin in lab now. Thanks, Kai Research Histotechnologist From gayle.callis at bresnan.net Mon Jul 27 18:21:38 2015 From: gayle.callis at bresnan.net (Gayle Callis) Date: Mon, 27 Jul 2015 17:21:38 -0600 Subject: [Histonet] Re. Decalcification with formic acid sodium Message-ID: <002501d0c8c2$fd295760$f77c0620$@bresnan.net> Dorothy and Carl, Comments about your Histonet replies on formic acid decalcification. The Morse solution referred to by Dorothy can be picked up online by typing in the DOI number: 10.1.1.4689.3439.pdf or title, Morse A. Formic acid-sodium citrate decalcification and butyl alcohol dehydration of teeth and bones for sectioning in paraffin. 1945 J Dental Res 1945:24:143. You will find the reference to Evans and Krajian paper on formic acid/sodium citrate along with the original recipe for their solution (equal parts of 85% (stock) formic acid and 20% sodium citrate). Morse modified the Evans Krajian method (1 part diluted stock formic acid i.e. 90% diluted 1:1 with water for 45% formic acid) plus 1 part 20% sodium citrate. The Morse paper was excellent and well worth reading. Interestingly, in 1962, our lab used the Morse solution for decalcifying teeth although it was never referred to by that name but simply formic acid/sodium citrate. The formic acid content in Morse's solution is half the concentration of formic acid in Evans/Krajian solution. It seems both work equally well and the higher concentration of formic acid should increase the decalcification rate somewhat. Morse also did chemical decalcification endpoint testing. Carl is correct about not mixing citric acid with formic acid as citric acid is not going to act as a buffer salt. However, you will find in the literature that citric acid is very mild and has been used as a decalcifying agent for EM studies. Carl is also correct in that sodium formate can be used as a buffering salt instead of sodium citrate. We have worked with both of the buffering salts/formic acid formulations and found they works equally well for decalcification. I have some publications on file comparing acid versus EDTA for cartilage and IHC studies and learned some researchers referred to buffered formic acid methods as "acidic buffers" . The latter terminology could be confusing to people in the business of decalcifying bones and teeth. but no more so than the acronyms manufacturers give their solutions. It pays to read the MSDS for any decalcifying solution, and even compare this information to what is in histology textbooks as part of our education. I have found the discussions on this topic enlightening. I will be happy to send the pdf of the Morse method to those interested in reading it. I have not been able to access the 1930 Evans Krajian method yet. What is important is knowing these older, classic formic acid methods are still tried and true with the added advantage of being available commercially for our convenience. Thanks everyone Gayle Callis You Wrote: There was a paper http://www.genedetect.com/Merchant2/ExampleRefs/Decalcifying_protocols.pdf Talking about formic acid (Morse solution) can get as good result as EDTA in ISH. FYI. Dorothy Hu Mouse knee joints: done lots of decalcified FFPWS for assessment of articular cartilage degeneration models. See Histonet images for a Tol blue image. Decal in 10 % EDTA for 3 days on a rocker at RT. Sure....5days if you are worried. No difference in Immuno-reactivity, imho. If you want to use buffered Formic acid, use Formic acid; sodium formate. Use of citric acid with Formic acid does not make a buffer. It's just mixing two relatively mild acids..... However, I am sure that Prof Kiernan can further enlighten us. Respectfully, Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 From kiran_g at sbcglobal.net Mon Jul 27 21:32:19 2015 From: kiran_g at sbcglobal.net (Kiran) Date: Mon, 27 Jul 2015 19:32:19 -0700 Subject: [Histonet] Dako Artisan Special Stainer In-Reply-To: References: Message-ID: Thank you! All for your feedback. It will definitely help us make better decision for our lab. In addition to quality & user friendly, we need this to work with our LIS / vantage tracking system...need it IT friendly too! Nothing is plug and play anymore... Kiran > On Jul 27, 2015, at 11:27 AM, deGuzman, Jose R via Histonet wrote: > > Hi all, > > We recently upgraded our old Artisan to the Artisan Link Pro. It does the depar. within the system itself. Set-up was easy, we just transferred our old protocols to the new units. Modifying protocols was easy too. Staining is consistent, tech support and their specialists are reliable and share information from what other users do. If I have kit problems, a replacement kit is shipped overnight to the lab. Kits come in 50 test or 100 test, our GMS 100 test kit usually lasts us 1 month and staining is consistent. We run multiple protocols per run and found that certain combinations can go together to save time such as: GMS, Reticulin and Jones for a 1 hour 20 minute stain and Trichrome, PAS to 1 hour, 40 minutes. Last month, we did 369 stains and 347 stains in May on this system. I print out usage reports by protocols and case lists including which unit performed the stain. A quick scan of the barcode lets me know the lot number and expiration date of the kit used. > > Hope this was helpful. > > Jose de Guzman > > > > MedStar Health is a not-for-profit, integrated healthcare delivery system, the largest in Maryland and the Washington, D.C., region. Nationally recognized for clinical quality in heart, orthopaedics, cancer and GI. IMPORTANT: This e-mail (including any attachments) may contain information that is private, confidential, or protected by attorney-client or other privilege. If you received this e-mail in error, please delete it from your system without copying it and notify sender by reply e-mail, so that our records can be corrected. Thank you. Help conserve valuable resources - only print this email if necessary. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan at uwo.ca Tue Jul 28 00:13:05 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Tue, 28 Jul 2015 00:13:05 -0500 Subject: [Histonet] Toluidine blue stain for MMA In-Reply-To: <7360b91b3d6af.55b70f24@uwo.ca> References: <11fca7646af29705683356042da527cf@waxitinc.com> <7280d3e53a52b.55b70b4a@uwo.ca> <7390cc6d39788.55b70b87@uwo.ca> <7360ca5a3a9a3.55b70bc5@uwo.ca> <7360c2083caf0.55b70c05@uwo.ca> <7360fecb3c03b.55b70c43@uwo.ca> <7360a47c3d442.55b70c81@uwo.ca> <7360eb253c8bd.55b70cb9@uwo.ca> <71e0c47239b8d.55b70d33@uwo.ca> <71e0826e3dca3.55b70d71@uwo.ca> <7370eaf53dd62.55b70daf@uwo.ca> <737086163f5c9.55b70ded@uwo.ca> <73708b923ca59.55b70e2b@uwo.ca> <73708de93c5c9.55b70e6a@uwo.ca> <7360b0af3d617.55b70ea8@uwo.ca> <7360fc013c8aa.55b70ee6@uwo.ca> <7360b91b3d6af.55b70f24@uwo.ca> Message-ID: <7360e3243a8bc.55b6c911@uwo.ca> Yes. Probably hundreds of Histonetters stain plastic sections. Let us all hope they don't all bombard the Histonet listserver with replies to your question. Instructions for staining plastic sections with toluidine blue are in every library that contains books with paper pages, and also (albeit with less authority) in great abundance on the Web. Try typing SEMITHIN STAIN into Google. I just did, and an excellent web site came up on top of the heap. John Kiernan = = = On 27/07/15, Kai Hong via Histonet wrote: > > Hi, > > is there anyone have an experience with MMA toluidine staining? > Im using T7200, T9100, Osteo-bed resin in lab now. > > Thanks, > Kai > Research Histotechnologist > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jkiernan at uwo.ca Tue Jul 28 11:06:29 2015 From: jkiernan at uwo.ca (John Kiernan) Date: Tue, 28 Jul 2015 11:06:29 -0500 Subject: [Histonet] axolotl lymphatics In-Reply-To: <72f0ec9ab73.55b7a858@uwo.ca> References: <1267660652.2411.1437958174751.JavaMail.root@zstore.medstv.unimelb.edu.au> <983727148.2470.1437958522140.JavaMail.root@zstore.medstv.unimelb.edu.au> <73c0b9866b1a.55b7a724@uwo.ca> <7340eaaa755e.55b7a760@uwo.ca> <735086fd1804.55b7a79e@uwo.ca> <7350af4e73bc.55b7a7dc@uwo.ca> <7350c0db5e90.55b7a81a@uwo.ca> <72f0ec9ab73.55b7a858@uwo.ca> Message-ID: <7340cf00689d.55b76235@uwo.ca> Instead of an antibody, you might consider enzyme activity histochemistry, which is much less expensive. Demonstration of 5-nucleotidase activity in the presence of levamisole detects lymphatic endothelium. Sections can also be stained for alkaline phosphatase activity in the endothelium of blood vessels. Here are a few references. Kato, S., Yasunaga, A. and Uchida, U. (1991). Enzyme-histochemical method for identification of lymphatic capillaries. Lymphology 24:125-129. Ohkuma, M. (1994). Simultaneous double staining for the blood and lymphatic capillary. Lymphology 27, Suppl:106-107. Okada, E. (1994). An improved enzyme-histochemical method for identification of lymphatic capillaries on paraffin sections. Lymphology 27, Suppl:732-735. Ji, R.C. and Kato, S. (2003). Lymphatic network and lymphangiogenesis in the gastric wall. Journal of Histochemistry and Cytochemistry 51:331-338. Needless to say, none of these relate to amphibian tissues! John Kiernan Anatomy, UWO, London, Canada = = = On 26/07/15, Jason Palmer via Histonet wrote: > > Hi all, > > I need to find an antibody that will label lymphatic endothelial cells in the axolotl. Does anybody have any experience or ideas? I have tried a couple of our anti-mouse and anti-human Abs for podoplanin and LYVE-1 but no cross-reactivity so far. I have no experience with staining of non-mammalian tissues - maybe an anti-frog Ab would cross react? Does anyone have experience with other amphibians? > > Thanks, > > Jason > > -- > > Jason Palmer > Histology Laboratory Coordinator > O'Brien Institute / St Vincent's Institute > 42 Fitzroy St, Fitzroy Victoria 3065 > Australia > tel +61 3 9288 4045 > fax +61 3 9416 0926 > email: jpalmer at svi.edu.au > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From shive003 at umn.edu Tue Jul 28 12:05:57 2015 From: shive003 at umn.edu (Jan Shivers) Date: Tue, 28 Jul 2015 12:05:57 -0500 Subject: [Histonet] EMA/MUC1 IHC on dogs Message-ID: Does anyone perform EMA/MUC1 IHC on canine tissue? If so, what company is your vendor? You can reply to me in a private message. Thanks in advance. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003 at umn.edu From tgenade at gmail.com Tue Jul 28 13:41:06 2015 From: tgenade at gmail.com (Tyrone Genade) Date: Tue, 28 Jul 2015 13:41:06 -0500 Subject: [Histonet] Re. Decalcification with formic acid sodium Message-ID: It isn't very clear, but for the Formic Acid/sodium Citrate buffer you have to use trisodium citrate. 10 g of disodium or monosodium citrate wouldn't work as well... -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From melissa at alliedsearchpartners.com Tue Jul 28 15:25:10 2015 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Tue, 28 Jul 2015 20:25:10 +0000 Subject: [Histonet] Direct Hire/Perm Histotech Job in State of Washington Message-ID: Hello, I have a great position in the state of Washington for a full time histotech in Walla Walla. Please email me for details. I am looking for a direct hire/permanent candidate. Thanks! Melissa Owens (Phelan) President, Laboratory Staffing Allied Search Partners From Karen.Heckford at DignityHealth.org Wed Jul 29 10:01:31 2015 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Wed, 29 Jul 2015 08:01:31 -0700 Subject: [Histonet] Breast fixation Message-ID: Good Morning, I have a question about breast fixation. I am in a little bit of a pickle with the 6-72 hour rule for the fixation on breast tissue. Friday I am getting 2 breast cases in the afternoon and both will not have the required minimum 6 hour formalin fixation for my per diem to cut early Saturday morning. He will not be able to make it in again until Monday night. The tissue will be about 3-4 hours (this includes time on the processor) over the 72 hour maximum. Does anyone have any suggestions on what can be done? We are a one person show here. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." From emilydewar32144 at gmail.com Wed Jul 29 10:10:19 2015 From: emilydewar32144 at gmail.com (Emily Dewar) Date: Wed, 29 Jul 2015 12:10:19 -0300 Subject: [Histonet] NetWell inserts and IHC with TUNEL stain Message-ID: Hello, I will be performing a number of immunos within the next couple of months, and have been wondering what the best way to do so might be with no tissue damage during the process. Does anyone know whether using NetWell inserts for immunos to transfer tissue affects morphology? The tissue will be placed into the insert, and submersed in solution within a well plate to be incubated on a shaker. The problem is that contact with the NetWell insert could damage the tissue, not only with the rocking, but with transfer from one solution to another. I am under the impression that with TUNEL staining, is often difficult to differentiate whether the damage in cells caused by handling/extraction, or from the treatment itself. While not impossible, I will not have the time to develop or perform such a procedure. If anyone has any knowledge or insight, it would be greatly appreciated. Thank you for your time, Emily Dewar Laboratory Technician From Marcie.Janineh at childrens.harvard.edu Wed Jul 29 13:14:51 2015 From: Marcie.Janineh at childrens.harvard.edu (Janineh, Marcie) Date: Wed, 29 Jul 2015 18:14:51 +0000 Subject: [Histonet] Cerebro or AB&T along with Cerner Millennium Message-ID: <33A991D932E6D14FA0F083BD6F64148E6B6BF184@CHEXMBX1A.CHBOSTON.ORG> Anyone using either AB&T or Cerebro along with Cerner Millennium? If so, would you be willing to share your experiences? Thanks in advance, Marcie From craigak12 at gmail.com Wed Jul 29 14:54:17 2015 From: craigak12 at gmail.com (Jb) Date: Wed, 29 Jul 2015 12:54:17 -0700 Subject: [Histonet] Formalin Neutralization: Message-ID: Does anyone have logs/procedure that they can share to document/explain formalin neutralization and disposal? Also curious how people are logging/documenting tissue dumps? Thank you, Craig Sent from my iPhone From lucy.zong at gmail.com Wed Jul 29 15:08:03 2015 From: lucy.zong at gmail.com (Lucy Zong) Date: Wed, 29 Jul 2015 16:08:03 -0400 Subject: [Histonet] CYtechThin Prep Message-ID: From lucy.zong at gmail.com Wed Jul 29 15:16:11 2015 From: lucy.zong at gmail.com (Lucy Zong) Date: Wed, 29 Jul 2015 16:16:11 -0400 Subject: [Histonet] Cytyc thin prep 2000 Message-ID: Our lab looking to have unit PM'd. Anyone have idea of what cost involved and name of someone who can do work. From TanyaAbbott at catholichealth.net Wed Jul 29 15:54:16 2015 From: TanyaAbbott at catholichealth.net (Abbott, Tanya) Date: Wed, 29 Jul 2015 20:54:16 +0000 Subject: [Histonet] Slide label printer Message-ID: <852F7D2C14FB464D80E182B15DB138AF6B8029B7@CHIEX005.CHI.catholichealth.net> I am looking for a slide label printer that is compatible with Meditech, any suggestions? If so, also any suggestions on what label stock/size to use that obviously can go through chemicals/staining? Thanks in advance! Tanya Tanya G. Abbott Manager Technologist Histology/Cytology Penn State Health St. Joseph (phone) 610-378-2635 This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From jclark at pcnm.com Wed Jul 29 16:01:27 2015 From: jclark at pcnm.com (Joanne Clark) Date: Wed, 29 Jul 2015 21:01:27 +0000 Subject: [Histonet] Breast Fixation In-Reply-To: References: Message-ID: <0494A7D4E8CC254EA2FB81464982E378D9AB6E45@S10MAILD001N3.SH10.lan> I would do a delayed start on your tissue processor Friday night to include the extra two hours you need of fixation time and just have the run come off two hours later on Saturday morning. Just adjust the hours of your per diem Saturday tech to come in later. Joanne Clark, HT Director of Histology Pathology Consultants of New Mexico Message: 4 Date: Wed, 29 Jul 2015 08:01:31 -0700 From: "Heckford, Karen - SMMC-SF" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Breast fixation Message-ID: Content-Type: text/plain; charset="us-ascii" Good Morning, I have a question about breast fixation. I am in a little bit of a pickle with the 6-72 hour rule for the fixation on breast tissue. Friday I am getting 2 breast cases in the afternoon and both will not have the required minimum 6 hour formalin fixation for my per diem to cut early Saturday morning. He will not be able to make it in again until Monday night. The tissue will be about 3-4 hours (this includes time on the processor) over the 72 hour maximum. Does anyone have any suggestions on what can be done? We are a one person show here. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ------------------------------ Message: 5 Date: Wed, 29 Jul 2015 12:10:19 -0300 From: Emily Dewar To: histonet at lists.utsouthwestern.edu Subject: [Histonet] NetWell inserts and IHC with TUNEL stain Message-ID: Content-Type: text/plain; charset=UTF-8 Hello, I will be performing a number of immunos within the next couple of months, and have been wondering what the best way to do so might be with no tissue damage during the process. Does anyone know whether using NetWell inserts for immunos to transfer tissue affects morphology? The tissue will be placed into the insert, and submersed in solution within a well plate to be incubated on a shaker. The problem is that contact with the NetWell insert could damage the tissue, not only with the rocking, but with transfer from one solution to another. I am under the impression that with TUNEL staining, is often difficult to differentiate whether the damage in cells caused by handling/extraction, or from the treatment itself. While not impossible, I will not have the time to develop or perform such a procedure. If anyone has any knowledge or insight, it would be greatly appreciated. Thank you for your time, Emily Dewar Laboratory Technician ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://cp.mcafee.com/d/k-Kr6h8idEIcL8CzB55AsrKrhKyYO-ev7fCQrELcLzD4QjqdQnCnNPUVMSztZxN5xCVEVdydzZyl1iREaYKrmJ1nBPqavTd791Z_HYCyCO-esWZOWqbz7cccT7cfsJt6OaaJQSel3PWApmU6CS3r1K_9Tso73xParNKVI06vaAWsTeBmVAP7QDYotW3u2qKMM-l9OwXn2sGjG3jpel9R1FsxlK5LE2zVkDjCVQGTcCo-A_z3LgrdLcee6MLaAWwQQg1QyfQC0pEw3djPh0yNs0Ph06QETVEwphdII6N00eEd9Y ------------------------------ End of Histonet Digest, Vol 140, Issue 31 ***************************************** Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From garreyf at gmail.com Wed Jul 29 16:39:28 2015 From: garreyf at gmail.com (Garreyf) Date: Wed, 29 Jul 2015 17:39:28 -0400 Subject: [Histonet] Breast Fixation In-Reply-To: <0494A7D4E8CC254EA2FB81464982E378D9AB6E45@S10MAILD001N3.SH10.lan> References: <0494A7D4E8CC254EA2FB81464982E378D9AB6E45@S10MAILD001N3.SH10.lan> Message-ID: I agree. That's the easiest solution. My colleague at a sister institution does not allow breast excisions on Friday. We need more people to do studies on fixation beyond 72 hours. My hunch is that another 24 hours won't affect ER , Pgr, and Her2 results. Garrey Sent from my iPhone > On Jul 29, 2015, at 5:01 PM, Joanne Clark via Histonet wrote: > > > I would do a delayed start on your tissue processor Friday night to include the extra two hours you need of fixation time and just have the run come off two hours later on Saturday morning. Just adjust the hours of your per diem Saturday tech to come in later. > > Joanne Clark, HT > Director of Histology > Pathology Consultants of New Mexico > > > Message: 4 > Date: Wed, 29 Jul 2015 08:01:31 -0700 > From: "Heckford, Karen - SMMC-SF" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Breast fixation > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > I have a question about breast fixation. I am in a little bit of a pickle with the 6-72 hour rule for the fixation on breast tissue. Friday I am getting 2 breast cases in the afternoon and both will not have the required minimum 6 hour formalin fixation for my per diem to cut early Saturday morning. He will not be able to make it in again until Monday night. The tissue will be about 3-4 hours (this includes time on the processor) over the 72 hour maximum. Does anyone have any suggestions on what can be done? We are a one person show here. > > Thanks, > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford at dignityhealth.org > Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." > > > > > > ------------------------------ > > Message: 5 > Date: Wed, 29 Jul 2015 12:10:19 -0300 > From: Emily Dewar > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] NetWell inserts and IHC with TUNEL stain > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Hello, > > I will be performing a number of immunos within the next couple of months, > and have been wondering what the best way to do so might be with no tissue > damage during the process. Does anyone know whether using NetWell inserts > for immunos to transfer tissue affects morphology? The tissue will be > placed into the insert, and submersed in solution within a well plate to be > incubated on a shaker. The problem is that contact with the NetWell insert > could damage the tissue, not only with the rocking, but with transfer from > one solution to another. > > I am under the impression that with TUNEL staining, is often difficult to > differentiate whether the damage in cells caused by handling/extraction, or > from the treatment itself. While not impossible, I will not have the time > to develop or perform such a procedure. > > If anyone has any knowledge or insight, it would be greatly appreciated. > > Thank you for your time, > > Emily Dewar > Laboratory Technician > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://cp.mcafee.com/d/k-Kr6h8idEIcL8CzB55AsrKrhKyYO-ev7fCQrELcLzD4QjqdQnCnNPUVMSztZxN5xCVEVdydzZyl1iREaYKrmJ1nBPqavTd791Z_HYCyCO-esWZOWqbz7cccT7cfsJt6OaaJQSel3PWApmU6CS3r1K_9Tso73xParNKVI06vaAWsTeBmVAP7QDYotW3u2qKMM-l9OwXn2sGjG3jpel9R1FsxlK5LE2zVkDjCVQGTcCo-A_z3LgrdLcee6MLaAWwQQg1QyfQC0pEw3djPh0yNs0Ph06QETVEwphdII6N00eEd9Y > > ------------------------------ > > End of Histonet Digest, Vol 140, Issue 31 > ***************************************** > > > > Disclaimer: This electronic message may contain information that is proprietary, > confidential, or legally privileged or protected. It is intended only for the use > of the individual(s) and entity named in the message. If you are not an intended > recipient of this message, please notify the sender immediately and delete the > material from your computer. Do not deliver, distribute or copy this message and > do not disclose its contents or take any action in reliance on the information it > contains. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From modz9636 at gmail.com Wed Jul 29 17:33:07 2015 From: modz9636 at gmail.com (M.O.) Date: Wed, 29 Jul 2015 15:33:07 -0700 Subject: [Histonet] Safranin O/ Fast green - protocol without alcohol Message-ID: Histonetters! Our go-to stain is SafO for any bone/cartilage, but we always have the option to use toluidine or alcian blue. Just going out on a limb, but has anyone stained tissues with SafO/Fast Green, without using any alcohols after the SafO step? These samples have some issues when put into alcohol and if we wash the SafO in water, instead of alcohol, the stain isn't correct. What is it about the alcohol step after SafO that makes it work so well? And why does water cause such a problem? Next week, I will try a lower alcohol percentage after the SafO step to see how that works. Thanks, Merissa From christina.kreutzer01 at gmail.com Thu Jul 30 05:42:51 2015 From: christina.kreutzer01 at gmail.com (Christina Kreutzer) Date: Thu, 30 Jul 2015 12:42:51 +0200 Subject: [Histonet] Deyemond-Diamont Knife Message-ID: Hello everybody, I was curious, whether anybody of you has any experiences with Deyemond-Diamont Knifes, regarding durability etc. Apparently the company doesn't exist anymore, but we still have some knifes and I wanted to know what your opinions would be compared to other companies. Regards, Christina From ltougas at dawsoncollege.qc.ca Thu Jul 30 07:53:41 2015 From: ltougas at dawsoncollege.qc.ca (Liette Tougas) Date: Thu, 30 Jul 2015 12:53:41 +0000 Subject: [Histonet] ski wax iron Message-ID: <455897B94CF3A44F81F727160F23FB11640E6E28@DC229.ad.dawsoncollege.qc.ca> Hello Histonet world! (Fran?ais suit plus bas!) I know that some labs use a ski wax iron to remove the paraffin around blocks. Would any model in particular work best? We are a small Biomedical Lab. Tech. program and cannot afford the specific "wax trimmers" sold by companies which distribute histo equipement. Thank you in advance for the precious info! ________________________________ Bonjour! Je sais que certains labos utilisent un fer pour le cirage des skis, pour enlever le surplus de paraffine des blocs. Recommenderiez-vous un mod?le en particulier? Nous sommes un petit programme de TAB et ne pouvons nous permettre d'acheter les appareils specialis?s vendus par les compagnies qui distribuent de l'?quipement d'histo. Merci ? l'avance pour l'information! Liette Tougas, RT, B.Sc., M.Sc. Biomedical Laboratory Technology Department Dawson College, Montreal, Canada From Julia.Cates at AHSS.ORG Thu Jul 30 10:30:01 2015 From: Julia.Cates at AHSS.ORG (Cates, Julia) Date: Thu, 30 Jul 2015 15:30:01 +0000 Subject: [Histonet] Ventana H.Pylori antibody Message-ID: Good day Histonet, I have seen in the past conversations regarding Ventana's H.Pylori antibody and how dirty looking the stain can be. We have been using this antibody for several years now and have never really been happy with the quality. We have tried the recommended methods to "clean up" the stain and still we run into repeating the stain and/or complaints from the pathologists. We are considering using a different vendor but my concern is that my efforts will be a lateral move. Is anyone using a product that produces a clean stain or is this something inherent to this antibody? Are the pathologists happy with it and not just tolerating it? Thank you for your suggestions, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. From Mary.Leslie at tricore.org Thu Jul 30 11:14:48 2015 From: Mary.Leslie at tricore.org (Leslie, Mary) Date: Thu, 30 Jul 2015 16:14:48 +0000 Subject: [Histonet] Histonet Digest, Vol 140, Issue 9 In-Reply-To: References: Message-ID: Taking a poll: How many of your labs recycle formalin, alcohol and xylene? -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, July 09, 2015 11:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 140, Issue 9 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. 2 Open Positions in Little Rock Arkansas (Marcum, Pamela A) 2. Gross Room QC sheet (Histology Technician) 3. Medica ELGA DV 25 (Histology Technician) 4. Roche B-gal Staining Kit (Coffey, Anna (NIH/NCI) [C]) ---------------------------------------------------------------------- Message: 1 Date: Thu, 9 Jul 2015 15:15:47 +0000 From: "Marcum, Pamela A" To: Histonet Subject: [Histonet] 2 Open Positions in Little Rock Arkansas Message-ID: Content-Type: text/plain; charset="us-ascii" WE currently have two open positions for either registered HT or HTL. Registry must be current and some experience. We are a heavily computerized Histology Laboratory and will require the persons applying to be comfortable with computers. We are a routine Histology Laboratory at the University of Arkansas for Medical Sciences, in Little Rock. This is a beautiful area in Central Arkansas with lots of things to do both indoors and especially outdoors. If you are interested please contact me with your resume as soon as possible. Please no recruiters, we are not allowed to use you, I am sorry. Thank You, Pam Marcum ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 2 Date: Thu, 9 Jul 2015 16:20:09 +0000 (UTC) From: Histology Technician To: Histonet , "histonet-request at lists.utsouthwestern.edu" Subject: [Histonet] Gross Room QC sheet Message-ID: <1290933179.1997863.1436458809680.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Does anyone have a Gross Room QC sheet you'd like to share?? Thanks so much! ------------------------------ Message: 3 Date: Thu, 9 Jul 2015 16:22:47 +0000 (UTC) From: Histology Technician To: Histonet , "histonet-request at lists.utsouthwestern.edu" Subject: [Histonet] Medica ELGA DV 25 Message-ID: <377965148.2007366.1436458967925.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Does anyone use the DI water system called Medica by Eqova?? We just recently got 2 in our lab and was wondering if anyone had an SOP you'd like to share?? Can you tell my what daily QC needs to be done with it?? Water tested?? Thanks! ------------------------------ Message: 4 Date: Thu, 9 Jul 2015 16:57:14 +0000 From: "Coffey, Anna (NIH/NCI) [C]" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Roche B-gal Staining Kit Message-ID: <5C3E10119A1B824FBE92B08279F74A910259809B at msgb10.nih.gov> Content-Type: text/plain; charset="us-ascii" Hello, I have been asked to do a B-gal enzyme stain using a Roche kit on frozen mouse sections. Another histologist recommended post-fixing in a glutaraldehyde-formaldehyde solution although this isn't mentioned in the kit instructions. This has me worried that there may be other things missing from the kit instructions that could improve the results. Has anyone else had any experience using this kit and, if so, did you find that other alterations to the protocol were required? Also wondering about potential counterstains that might work with this one. I'd appreciate any pointers if you have them! Thanks! Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey at nih.gov 301-846-1730 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 140, Issue 9 **************************************** From JRobinson at pathology-associates.com Thu Jul 30 12:06:04 2015 From: JRobinson at pathology-associates.com (Jeffrey Robinson) Date: Thu, 30 Jul 2015 17:06:04 +0000 Subject: [Histonet] Ventana H.Pylori antibody In-Reply-To: References: Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D9EB26@PAEXCH1.PathologyAssociates.local> Hi Julia- I have had a lot of experience trying to get a clean H. pylori. I don't recall using the Ventana H. pylori but I was using the CellMarque H. pylori for years. It would often have a lot of background as you describe. I run Ventana Benchmarks (XT and Ultra) and a Leica Bond. The slides I would run on the Benchmarks were usually cleaner than those run on the Bond but still not as clean as I would like. I finally switched over to BioCare's H. pylori after many complaints from my pathologists. It has been much cleaner on all of my IHC instruments. Actually, the ones I run on the Ultra are the cleanest I have ever seen. The slides run on the Bond may still have a little background but it is still much cleaner than the ones I used to run with the CellMarque antibody. One of my pathologists who used to complain about the excessive background now calls it his "go to" stain and is very happy with how crisp it is. The organisms stand out very nicely. BioCare will send you a free sample if you want to try it. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Cates, Julia via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 30, 2015 8:30 AM To: histonet-bounces at lists.utsouthwestern.edu; histonet at lists.utsouthwestern.edu Subject: [Histonet] Ventana H.Pylori antibody Good day Histonet, I have seen in the past conversations regarding Ventana's H.Pylori antibody and how dirty looking the stain can be. We have been using this antibody for several years now and have never really been happy with the quality. We have tried the recommended methods to "clean up" the stain and still we run into repeating the stain and/or complaints from the pathologists. We are considering using a different vendor but my concern is that my efforts will be a lateral move. Is anyone using a product that produces a clean stain or is this something inherent to this antibody? Are the pathologists happy with it and not just tolerating it? Thank you for your suggestions, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From Valerie.Hannen at parrishmed.com Thu Jul 30 12:20:54 2015 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Thu, 30 Jul 2015 13:20:54 -0400 Subject: [Histonet] Histonet Digest, Vol 140, Issue 9 In-Reply-To: References: Message-ID: <450B7A81EDA0C54E97C53D60F00776C32337384283@isexstore03> We do. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Leslie, Mary via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 30, 2015 12:15 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 140, Issue 9 Taking a poll: How many of your labs recycle formalin, alcohol and xylene? -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, July 09, 2015 11:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 140, Issue 9 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. 2 Open Positions in Little Rock Arkansas (Marcum, Pamela A) 2. Gross Room QC sheet (Histology Technician) 3. Medica ELGA DV 25 (Histology Technician) 4. Roche B-gal Staining Kit (Coffey, Anna (NIH/NCI) [C]) ---------------------------------------------------------------------- Message: 1 Date: Thu, 9 Jul 2015 15:15:47 +0000 From: "Marcum, Pamela A" To: Histonet Subject: [Histonet] 2 Open Positions in Little Rock Arkansas Message-ID: Content-Type: text/plain; charset="us-ascii" WE currently have two open positions for either registered HT or HTL. Registry must be current and some experience. We are a heavily computerized Histology Laboratory and will require the persons applying to be comfortable with computers. We are a routine Histology Laboratory at the University of Arkansas for Medical Sciences, in Little Rock. This is a beautiful area in Central Arkansas with lots of things to do both indoors and especially outdoors. If you are interested please contact me with your resume as soon as possible. Please no recruiters, we are not allowed to use you, I am sorry. Thank You, Pam Marcum ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 2 Date: Thu, 9 Jul 2015 16:20:09 +0000 (UTC) From: Histology Technician To: Histonet , "histonet-request at lists.utsouthwestern.edu" Subject: [Histonet] Gross Room QC sheet Message-ID: <1290933179.1997863.1436458809680.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Does anyone have a Gross Room QC sheet you'd like to share?? Thanks so much! ------------------------------ Message: 3 Date: Thu, 9 Jul 2015 16:22:47 +0000 (UTC) From: Histology Technician To: Histonet , "histonet-request at lists.utsouthwestern.edu" Subject: [Histonet] Medica ELGA DV 25 Message-ID: <377965148.2007366.1436458967925.JavaMail.yahoo at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Does anyone use the DI water system called Medica by Eqova?? We just recently got 2 in our lab and was wondering if anyone had an SOP you'd like to share?? Can you tell my what daily QC needs to be done with it?? Water tested?? Thanks! ------------------------------ Message: 4 Date: Thu, 9 Jul 2015 16:57:14 +0000 From: "Coffey, Anna (NIH/NCI) [C]" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Roche B-gal Staining Kit Message-ID: <5C3E10119A1B824FBE92B08279F74A910259809B at msgb10.nih.gov> Content-Type: text/plain; charset="us-ascii" Hello, I have been asked to do a B-gal enzyme stain using a Roche kit on frozen mouse sections. Another histologist recommended post-fixing in a glutaraldehyde-formaldehyde solution although this isn't mentioned in the kit instructions. This has me worried that there may be other things missing from the kit instructions that could improve the results. Has anyone else had any experience using this kit and, if so, did you find that other alterations to the protocol were required? Also wondering about potential counterstains that might work with this one. I'd appreciate any pointers if you have them! Thanks! Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.coffey at nih.gov 301-846-1730 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 140, Issue 9 **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From MICHELLE.LAMPHERE at childrens.com Thu Jul 30 13:05:23 2015 From: MICHELLE.LAMPHERE at childrens.com (Michelle Lamphere) Date: Thu, 30 Jul 2015 18:05:23 +0000 Subject: [Histonet] Cerebro or AB&T along with Cerner Millennium (Janineh, Marcie) In-Reply-To: References: Message-ID: We have Cerner Millenium and just went live with AB&T. I do not know anything about Cerebro, but what I know of AB&T so far is that it is does not do a whole lot of things that I wish it did. Such as, I cannot easily generate any kind of report regarding the data (turn-around times) that is collected. I can go into each individual case and see each step of the process, but not any sort of collective data for a time/date range. Also, the support we have gotten from Cerner for the entire process has been lacking. We did eventually (towards the very end of the build) get a person who was responsive and helpful, but if we had not insisted on that, we would have had no training whatsoever. We were given a spreadsheet and told to fill in the blanks. We did not have a person from Cerner physically here when we went live. All problems have had to be addressed over the phone because the person that we do have on site is a contract Cerner person who had never had any knowledge of AP prior to this project. Whatever equipment you have that you will need to interface, I would make sure that you work those details out in advance. Cerner really tried to gouge us with pricing of interfaces. Everything is based on your current equipment, not the equipment that you have when the project actually goes live either. If you want to know more, feel free to contact me directly Michelle Lamphere Senior Tech, Histology Anatomic Pathology O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamphere at childrens.com 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 Message: 1 Date: Wed, 29 Jul 2015 18:14:51 +0000 From: "Janineh, Marcie" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Cerebro or AB&T along with Cerner Millennium Message-ID: <33A991D932E6D14FA0F083BD6F64148E6B6BF184 at CHEXMBX1A.CHBOSTON.ORG> Content-Type: text/plain; charset="us-ascii" Anyone using either AB&T or Cerebro along with Cerner Millennium? If so, would you be willing to share your experiences? Thanks in advance, Marcie Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at privacy at childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. From DEREK.WHEELER at readinghealth.org Thu Jul 30 13:36:03 2015 From: DEREK.WHEELER at readinghealth.org (Wheeler, Derek) Date: Thu, 30 Jul 2015 18:36:03 +0000 Subject: [Histonet] Organ harvest assessment Message-ID: <08FE3AB77A978E4FB4C2D2BAB39337C2A8D68542@TRH-EXMB08.trhmc.org> Trying to find out how other hospitals who do not have transplant services handle organ assessment requests from the pathology point of view. Do you provide frozen section coverage 24-7, Histologist and or Pathologist for the organ harvest assessment? If not 24-7 what services do you offer? Derek Wheeler Manager Anatomic Pathology derek.wheeler at readinghealth.org | readinghealth.org ________________________________ ----- Email Disclaimer ----- This email and any files transmitted with it are confidential and are intended for the named recipient(s). If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any copying, disclosure, dissemination, distribution or review of it or its contents is prohibited. If you have received this email in error, please notify the sender and immediately delete this email from your system. --------------------------------------- From Timothy.Morken at ucsf.edu Thu Jul 30 18:01:47 2015 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 30 Jul 2015 23:01:47 +0000 Subject: [Histonet] NFPA laser printer labels? Message-ID: <761E2B5697F795489C8710BCC72141FF60305BD7@ex07.net.ucsf.edu> Does anyone have a source for NFPA laser printer labels. Especially in the 1" x 3" size? Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From karenlahti3 at gmail.com Thu Jul 30 18:41:15 2015 From: karenlahti3 at gmail.com (Karen Lahti) Date: Thu, 30 Jul 2015 16:41:15 -0700 Subject: [Histonet] Ventana H.Pylori antibody In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D9EB26@PAEXCH1.PathologyAssociates.local> References: <204A03EB5A7F0A4BB1EEDD52A963829C16D9EB26@PAEXCH1.PathologyAssociates.local> Message-ID: Hi Julia, We have a GI lab and use Biocare's H. Pylori concentrate on Biocare's IntelliPath. We run about 50-75 H. pylori IHC each day. They are very clean and specific. Our Pathologists and myself are extremely happy with Biocare customer service and products. On Thursday, July 30, 2015, Cates, Julia via Histonet < histonet at lists.utsouthwestern.edu > wrote: > Good day Histonet, > > I have seen in the past conversations regarding Ventana's H.Pylori > antibody and how dirty looking the stain can be. We have been using this > antibody for several years now and have never really been happy with the > quality. We have tried the recommended methods to "clean up" the stain and > still we run into repeating the stain and/or complaints from the > pathologists. We are considering using a different vendor but my concern > is that my efforts will be a lateral move. Is anyone using a product that > produces a clean stain or is this something inherent to this antibody? Are > the pathologists happy with it and not just tolerating it? > > Thank you for your suggestions, > > Julia Cates, HT(ASCP)cm > Pathology Coordinator, Pathology > Florida Hospital Waterman > (352) 253-3333 ext.4346 | Fax: (352) 253-3592 > > Confidentiality Statement: This email message, including any attachments, > is for the sole use of the intended recipient and may contain confidential > and privileged information. Any unauthorized review, use, disclosure, or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply to this email and delete the original and all > copies of this email. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Thank You, Karen Lahti, HT ( ASCP), QIHC 480-236-6559 From jpalmer at svi.edu.au Fri Jul 31 00:07:36 2015 From: jpalmer at svi.edu.au (Jason Palmer) Date: Fri, 31 Jul 2015 15:07:36 +1000 (EST) Subject: [Histonet] axolotl lymphatics In-Reply-To: <7340cf00689d.55b76235@uwo.ca> Message-ID: <643134855.7815.1438319256148.JavaMail.root@zstore.medstv.unimelb.edu.au> Thankyou for your reply John. There is no-one whose suggestions I value more on Histonet, and your book (H and H methods) is also my go-to source for all things staining-related. I didn't mention in my post that the material I am working with is paraffin-embedded, so will not be any good for enzyme demonstration. If I can manage to get some tissue for freezing at some point then I will consider your suggestions. From memory, I tried something similar 20-odd years back on frozens, before the advent of podoplanin, LYVE-1 etc. immunostaining for lymphatics on FFPE, but without much success. Might be worth a re-visit. Jason ----- Original Message ----- From: "John Kiernan" To: "Jason Palmer" , histonet at lists.utsouthwestern.edu Sent: Wednesday, 29 July, 2015 2:06:29 AM Subject: Re: [Histonet] axolotl lymphatics Instead of an antibody, you might consider enzyme activity histochemistry, which is much less expensive. Demonstration of 5-nucleotidase activity in the presence of levamisole detects lymphatic endothelium. Sections can also be stained for alkaline phosphatase activity in the endothelium of blood vessels. Here are a few references. Kato, S., Yasunaga, A. and Uchida, U. (1991). Enzyme-histochemical method for identification of lymphatic capillaries. Lymphology 24 :125-129. Ohkuma, M. (1994). Simultaneous double staining for the blood and lymphatic capillary. Lymphology 27 , Suppl:106-107. Okada, E. (1994). An improved enzyme-histochemical method for identification of lymphatic capillaries on paraffin sections. Lymphology 27 , Suppl:732-735. Ji, R.C. and Kato, S. (2003). Lymphatic network and lymphangiogenesis in the gastric wall. Journal of Histochemistry and Cytochemistry 51 :331-338. Needless to say, none of these relate to amphibian tissues! John Kiernan Anatomy, UWO, London, Canada = = = On 26/07/15, Jason Palmer via Histonet wrote: Hi all, I need to find an antibody that will label lymphatic endothelial cells in the axolotl. Does anybody have any experience or ideas? I have tried a couple of our anti-mouse and anti-human Abs for podoplanin and LYVE-1 but no cross-reactivity so far. I have no experience with staining of non-mammalian tissues - maybe an anti-frog Ab would cross react? Does anyone have experience with other amphibians? Thanks, Jason -- Jason Palmer Histology Laboratory Coordinator O'Brien Institute / St Vincent's Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4045 fax +61 3 9416 0926 email: jpalmer at svi.edu.au _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MWhite at mhs.net Fri Jul 31 12:09:00 2015 From: MWhite at mhs.net (White, Marcia) Date: Fri, 31 Jul 2015 17:09:00 +0000 Subject: [Histonet] Ventana H.Pylori antibody In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D9EB26@PAEXCH1.PathologyAssociates.local> References: <204A03EB5A7F0A4BB1EEDD52A963829C16D9EB26@PAEXCH1.PathologyAssociates.local> Message-ID: <8B4B5BF3FCB6D24CBEAA63ACA5FDAD3B51490B6F@MHSEXMB04.mhs.net> We also are having the same problem with the Ventana H.Pylori antibody on our Ultras, I have tried the CellMarque product in the past and as mentioned still not ideal. Will try the Biocare product and see if the results improve for us also. Marcia M White Pathology Manager Memorial Regional Hospital 954-265-5371-office 954-967-7627-fax MWhite at mhs.net -----Original Message----- From: Jeffrey Robinson [mailto:JRobinson at pathology-associates.com] Sent: Thursday, July 30, 2015 1:06 PM To: Cates, Julia; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana H.Pylori antibody Hi Julia- I have had a lot of experience trying to get a clean H. pylori. I don't recall using the Ventana H. pylori but I was using the CellMarque H. pylori for years. It would often have a lot of background as you describe. I run Ventana Benchmarks (XT and Ultra) and a Leica Bond. The slides I would run on the Benchmarks were usually cleaner than those run on the Bond but still not as clean as I would like. I finally switched over to BioCare's H. pylori after many complaints from my pathologists. It has been much cleaner on all of my IHC instruments. Actually, the ones I run on the Ultra are the cleanest I have ever seen. The slides run on the Bond may still have a little background but it is still much cleaner than the ones I used to run with the CellMarque antibody. One of my pathologists who used to complain about the excessive background now calls it his "go to" stain and is very happy with how crisp it is. The organisms stand out very nicely. BioCare will send you a free sample if you want to try it. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Cates, Julia via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 30, 2015 8:30 AM To: histonet-bounces at lists.utsouthwestern.edu; histonet at lists.utsouthwestern.edu Subject: [Histonet] Ventana H.Pylori antibody Good day Histonet, I have seen in the past conversations regarding Ventana's H.Pylori antibody and how dirty looking the stain can be. We have been using this antibody for several years now and have never really been happy with the quality. We have tried the recommended methods to "clean up" the stain and still we run into repeating the stain and/or complaints from the pathologists. We are considering using a different vendor but my concern is that my efforts will be a lateral move. Is anyone using a product that produces a clean stain or is this something inherent to this antibody? Are the pathologists happy with it and not just tolerating it? Thank you for your suggestions, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. From Teresajharris at msn.com Fri Jul 31 12:18:00 2015 From: Teresajharris at msn.com (Teresa Harris) Date: Fri, 31 Jul 2015 13:18:00 -0400 Subject: [Histonet] (no subject) Message-ID: Yes Sent from my iPhone From alkhenaizik at gmail.com Fri Jul 31 14:14:43 2015 From: alkhenaizik at gmail.com (kalkhenaizi .) Date: Fri, 31 Jul 2015 22:14:43 +0300 Subject: [Histonet] P16 Message-ID: Hello Histonetters, Happy Friday! I need p16 antibody but not sure which vendor provide reliable antibody. I'd appreciate any recommendation. I also need melanoma and prostate ca control blocks, I can exchange with the once I have. Thank you and have a nice weekend. Kadhem Kadhem Alkhenaizi, MS HTL (ASCP) MB, QIHC Lab Manager ExpressMed Labs From Richard.Cartun at hhchealth.org Fri Jul 31 14:28:44 2015 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 31 Jul 2015 19:28:44 +0000 Subject: [Histonet] Breast fixation In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E6B01855A@HHCEXCHMB03.hhcsystem.org> There are several studies (one listed below) that have addressed the issue of fixation past the "recommended" 72 hour maximum. In my opinion, the science behind these fixation recommendations is "weak" at best. In my own experience, I have seen no problem whatsoever going well past 72 hours of fixation. The majority of breast cases will show internal positive controls for ER and PR to validate that the tissue is satisfactory for testing. Remember, working with your pathologist(s) you can perform your own validation on-site for extended fixation time. The real culprit for poor ER, PR, and HER2 IHC results is "inadequate" fixation. Reference: Tong LC, Nelson N, Tsourigiannis J, et al.: The effect of prolonged fixation on the IHC evaluation of ER, PR, and HER2 expression in invasive breast cancer: A prospective study. Am J Surg Pathol 2011;35:545-552. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, July 29, 2015 11:02 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Breast fixation Good Morning, I have a question about breast fixation. I am in a little bit of a pickle with the 6-72 hour rule for the fixation on breast tissue. Friday I am getting 2 breast cases in the afternoon and both will not have the required minimum 6 hour formalin fixation for my per diem to cut early Saturday morning. He will not be able to make it in again until Monday night. The tissue will be about 3-4 hours (this includes time on the processor) over the 72 hour maximum. Does anyone have any suggestions on what can be done? We are a one person show here. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Julia.Cates at AHSS.ORG Fri Jul 31 14:56:27 2015 From: Julia.Cates at AHSS.ORG (Cates, Julia) Date: Fri, 31 Jul 2015 19:56:27 +0000 Subject: [Histonet] Ventana H.Pylori antibody Message-ID: Wow! Thank you all so much for your responses. They have been very helpful; it's so great to have a community of professionals that are so willing to give of their knowledge. Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole?use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited.? If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. Message: 1 Date: Thu, 30 Jul 2015 17:06:04 +0000 From: Jeffrey Robinson To: "Cates, Julia" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Ventana H.Pylori antibody Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D9EB26 at PAEXCH1.PathologyAssociates.local> Content-Type: text/plain; charset="us-ascii" Hi Julia- I have had a lot of experience trying to get a clean H. pylori. I don't recall using the Ventana H. pylori but I was using the CellMarque H. pylori for years. It would often have a lot of background as you describe. I run Ventana Benchmarks (XT and Ultra) and a Leica Bond. The slides I would run on the Benchmarks were usually cleaner than those run on the Bond but still not as clean as I would like. I finally switched over to BioCare's H. pylori after many complaints from my pathologists. It has been much cleaner on all of my IHC instruments. Actually, the ones I run on the Ultra are the cleanest I have ever seen. The slides run on the Bond may still have a little background but it is still much cleaner than the ones I used to run with the CellMarque antibody. One of my pathologists who used to complain about the excessive background now calls it his "go to" stain and is very happy with how crisp it is. The organisms stand out very nicely. BioCare will send you a free sample if you want to try it. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: Cates, Julia via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 30, 2015 8:30 AM To: histonet-bounces at lists.utsouthwestern.edu; histonet at lists.utsouthwestern.edu Subject: [Histonet] Ventana H.Pylori antibody Good day Histonet, I have seen in the past conversations regarding Ventana's H.Pylori antibody and how dirty looking the stain can be. We have been using this antibody for several years now and have never really been happy with the quality. We have tried the recommended methods to "clean up" the stain and still we run into repeating the stain and/or complaints from the pathologists. We are considering using a different vendor but my concern is that my efforts will be a lateral move. Is anyone using a product that produces a clean stain or is this something inherent to this antibody? Are the pathologists happy with it and not just tolerating it? Thank you for your suggestions, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. _______________________________________________