From carl.hobbs <@t> kcl.ac.uk Thu Jan 1 12:29:22 2015 From: carl.hobbs <@t> kcl.ac.uk (Hobbs, Carl) Date: Thu Jan 1 12:29:29 2015 Subject: [Histonet] Re: thanks (Truscott, Tom) In-Reply-To: <2a92cdd4553f42d2bd0bd4c4238ffeaa@DB3FFO11FD055.protection.gbl> References: <2a92cdd4553f42d2bd0bd4c4238ffeaa@DB3FFO11FD055.protection.gbl> Message-ID: <1420136961502.35105@kcl.ac.uk> All the very best, Tom. I checked out where you worked....seems an essential service that can't be replaced. Sure....privatised? I don't know you but....losing/discarding essential skills is awful. I hope you are as successful in the future as you have been in the past. Respectfully, Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of histonet-request@lists.utsouthwestern.edu Sent: 01 January 2015 18:03 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 134, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. thanks (Truscott, Tom) ---------------------------------------------------------------------- Message: 1 Date: Wed, 31 Dec 2014 19:42:53 +0000 From: "Truscott, Tom" Subject: [Histonet] thanks To: "'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu)" Message-ID: <9EF5279EBDFE6E4FB6605E8F183A0027D69C61C8@CVM76.vetmed.wsu.edu> Content-Type: text/plain; charset="us-ascii" Many thanks to all who contribute and keep the histonet running. I'm working my last day here at USDA-ARS ADRU within WSU which has been a very rewarding and enjoyable occupation. I was honored to be on 14 publications in 12 years with a few more in the works. Due to Federal budget constraints, they don't plan on replacing me with another BS An. Sci. ASCP HT. Happy New Year to you all! Thanks again Tom T= Thomas C. Truscott ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 134, Issue 1 **************************************** From bburnett <@t> CapeCodHealth.org Fri Jan 2 08:40:23 2015 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Fri Jan 2 08:41:37 2015 Subject: [Histonet] HER2 IHC score averages Message-ID: Would anyone happen to know the overall scoring averages for HER2 IHC for each score (0, 1+, 2+, 3+)? ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From joewalker <@t> rrmc.org Fri Jan 2 09:04:39 2015 From: joewalker <@t> rrmc.org (Joe W. Walker, Jr.) Date: Fri Jan 2 09:04:44 2015 Subject: [Histonet] RE: Giemsa - smears vs. paraffin sections In-Reply-To: References: Message-ID: <3C2378778400AD448ADA6FD6BDB7CCCC187DDD36@RRMBX01.rrmc.local> Hi Elizabeth, The intent of Wright Giemsa and MGG is to generally highlight more of the nuclear and cytoplasmic features that may not be as easily observed in fixed specimens stained with H&E or another alcohol based stain like the Pap. Most all Romanowsky type stains of which Wright's, Giemsa and MGG are variations have a preparation step that involves placing material on a slide allowing the specimen to "air-dry" before being placed in methanol. This air drying creates an artificial enlargement of both nuclear and cytoplasmic items, which are highlighted by these types of stains. In my experience, you have to be pretty careful when applying these stains to formalin fixed tissues like bone marrows since the expected staining pattern is altered due to the steps needed in order to stain fixed tissue. Typically the nuclei appear blue instead of purple and makes it difficult to distinguish nucleoli that normally stain blue in cells. As to the differences between Wright Giemsa and MGG, I believe this is a matter of preference as both would produce similar results. We have run each stain on specimens in blinded studies in our own lab and our pathologists and cytotechnologists prefer the MGG stain. The preference is hard to quantitate since it is all qualitative responses. I am unware of any literature that supports one over the other for different specimen preparations. Cheers, Joe W. Walker, Jr. MS, SCT(ASCP)CM Manager of Anatomical Pathology, Microbiology and Reference Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 Email joewalker@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition? and the Governor's Award for Performance Excellence -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cameron, Elizabeth Sent: Wednesday, December 31, 2014 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Giemsa - smears vs. paraffin sections Happy New Year, Histonetters! I have always been taught that a Wright-Giemsa stain was strictly for smears, and a different modification (i.e. May-Grunwald) should be used for tissues. I was recently asked what the reasoning was, and to be honest I am not sure. I understand that tissue stains differently from smears, and I would imagine timing would be different, but is there a reason the solutions are different? Very curious. Thanks! -Liz _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From Richard.Cartun <@t> hhchealth.org Fri Jan 2 11:08:17 2015 From: Richard.Cartun <@t> hhchealth.org (Cartun, Richard) Date: Fri Jan 2 11:08:25 2015 Subject: [Histonet] RE: HER2 IHC score averages In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E3804B956@HHCEXCHMB03.hhcsystem.org> I haven't looked at my 2014 data yet, but I can give you scores for the first quarter of 2013: 118 cases of invasive carcinoma were tested (clone EP3) 18 (15.3%) were "Positive" (3+) by IHC 7 (5.9%) were "Equivocal" (2+) by IHC 93 (78.8%) were "Negative (0 or 1+) by IHC With the new 2014 HER2 interpretation guidelines I would predict that we had more "1+" and "2+" cases, and less "0" cases in 2014. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Friday, January 02, 2015 9:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HER2 IHC score averages Would anyone happen to know the overall scoring averages for HER2 IHC for each score (0, 1+, 2+, 3+)? ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Nancy_Schmitt <@t> pa-ucl.com Fri Jan 2 12:23:26 2015 From: Nancy_Schmitt <@t> pa-ucl.com (Nancy Schmitt) Date: Fri Jan 2 12:23:34 2015 Subject: [Histonet] acid fast control tissue Message-ID: <906B4DA90ED1DB4DB6C7E94D7CEE6C360115962F35@PEITHA.wad.pa-ucl.com> Happy New Year! My current source for acid fast control tissue is no longer available. I would appreciate any direction....... Thank you- Nancy Nancy Schmitt MLT, HT(ASCP) Histology Coordinator United Clinical Laboratories Dubuque, IA 52001 ph. 563-690-4142 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From Kelly.Pairan <@t> nationwidechildrens.org Fri Jan 2 12:39:42 2015 From: Kelly.Pairan <@t> nationwidechildrens.org (Pairan, Kelly) Date: Fri Jan 2 12:39:48 2015 Subject: [Histonet] On-line Bachelor Degrees for Histotechs Message-ID: <10EFB578346B744AB255AA4972B919506926C946@L1PERDWXMB04.childrensroot.net> Happy New Year Histo World, I graduated from a 3 year Medical Laboratory Science program in Canada in 2002 and have been employed in a hospital lab ever since. I began working full time in histology in 2005 when I moved to the States. I am currently working in a lab that I love, under a great manager at a great hospital. The only problem is that I have reached a point in my career at this hospital where I will need a bachelor's degree to be eligible for promotion. I know that there are several schools that have on-line MLS degree programs for MLT's who are working in the lab but I really don't want to go back to working in the core lab just to get a bachelor's degree. Does anyone know of a reputable school that has on-line degrees for histotechs or something like that? I would welcome anyone's advice. Thanks, Kelly From tbilaboratory <@t> gmail.com Fri Jan 2 13:24:23 2015 From: tbilaboratory <@t> gmail.com (TBI Techs) Date: Fri Jan 2 13:24:29 2015 Subject: [Histonet] QA and QC Logs Message-ID: Hello, How long should QA and/or QC logs be held for? Thank you From latecor <@t> adinet.com.uy Fri Jan 2 13:57:02 2015 From: latecor <@t> adinet.com.uy (C.D.G.) Date: Fri Jan 2 13:56:42 2015 Subject: [Histonet] RE: HER2 IHC score averages In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2E3804B956@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2E3804B956@HHCEXCHMB03.hhcsystem.org> Message-ID: <54A6F80E.4030407@adinet.com.uy> In Uruguay the 3+ population is in the range 10-11%. Carlos Defeo Histotechnologist El 02/01/2015 a las 15:08, Cartun, Richard escribi?: > I haven't looked at my 2014 data yet, but I can give you scores for the first quarter of 2013: > > 118 cases of invasive carcinoma were tested (clone EP3) > 18 (15.3%) were "Positive" (3+) by IHC > 7 (5.9%) were "Equivocal" (2+) by IHC > 93 (78.8%) were "Negative (0 or 1+) by IHC > > With the new 2014 HER2 interpretation guidelines I would predict that we had more "1+" and "2+" cases, and less "0" cases in 2014. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy > Sent: Friday, January 02, 2015 9:40 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HER2 IHC score averages > > Would anyone happen to know the overall scoring averages for HER2 IHC for > > each score (0, 1+, 2+, 3+)? > > ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ > This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. > > Helpdesk@CapeCodHealth.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ian.bernard <@t> comcast.net Fri Jan 2 15:38:40 2015 From: ian.bernard <@t> comcast.net (ian bernard) Date: Fri Jan 2 15:39:00 2015 Subject: [Histonet] QA and QC Logs In-Reply-To: References: Message-ID: <019001d026d4$7a1f7ab0$6e5e7010$@comcast.net> Per CAP as working documents, 2 years. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of TBI Techs Sent: Friday, January 02, 2015 12:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QA and QC Logs Hello, How long should QA and/or QC logs be held for? Thank you _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From info <@t> biopathx.com Fri Jan 2 18:54:18 2015 From: info <@t> biopathx.com (BioPathx) Date: Fri Jan 2 18:54:27 2015 Subject: [Histonet] acid fast control tissue In-Reply-To: <906B4DA90ED1DB4DB6C7E94D7CEE6C360115962F35@PEITHA.wad.pa-ucl.com> References: <906B4DA90ED1DB4DB6C7E94D7CEE6C360115962F35@PEITHA.wad.pa-ucl.com> Message-ID: <555498914.8502449.1420246458132.open-xchange@bosoxweb04.eigbox.net> Dear Nancy: Happy New Year! We have acid fast control tissue for sale at Biopathx.com. You may email us at info@biopathx.com. Acid fast control tissue has not been listed on our website yet, we will email you detailed information. You may find other control tissues on our website at www.biopathx.com. Best Regards Alice BiopathxEmail: info@biopathx.com Website: www.biopathx.com > On January 2, 2015 at 1:23 PM Nancy Schmitt wrote: > > > Happy New Year! > > My current source for acid fast control tissue is no longer available. I would > appreciate any direction....... > > Thank you- > > Nancy > > Nancy Schmitt MLT, HT(ASCP) > Histology Coordinator > United Clinical Laboratories > Dubuque, IA 52001 > ph. 563-690-4142 > > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Sat Jan 3 14:29:01 2015 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Sat Jan 3 14:29:05 2015 Subject: [Histonet] Re: acid fast control tissue Message-ID: Best acid fast control tissue I ever saw came from rhesus monkeys imported from India for research in the 1960s. A lot of them had active tuberculosis and were put down and autopsied (by the veterinary pathologists at Johns Hopkins). This was human tuberculosis, from tissue very similar to human lung. There must be some way to get this material today. Something I've long wanted to know: does Mycobacterium avium-intracellulare (MAI) infected tissue have the same acid-fast staining properties as Myco. tuberculosis? - We all know that Myco. leprae doesn't. Bob Richmond Samurai Pathologist Maryville TN From hans <@t> histologistics.com Sat Jan 3 15:00:10 2015 From: hans <@t> histologistics.com (Hans B Snyder) Date: Sat Jan 3 15:00:14 2015 Subject: [Histonet] Looking for 2 Gram pos/neg blocks Message-ID: Hello All, I'm almost out of my gram pos/neg block. Would anyone be willing to trade for other types of tissue? I have AFB, iron, copper, amyloid. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com From classicdoc <@t> gmail.com Sat Jan 3 17:08:20 2015 From: classicdoc <@t> gmail.com (Douglas Gregg) Date: Sat Jan 3 17:08:27 2015 Subject: [Histonet] cutting honey bees Message-ID: Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist From rjr6 <@t> psu.edu Sat Jan 3 17:15:33 2015 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Sat Jan 3 17:15:40 2015 Subject: [Histonet] cutting honey bees In-Reply-To: References: Message-ID: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classicdoc@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbmphoto <@t> gmail.com Sat Jan 3 20:01:55 2015 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Sat Jan 3 20:02:04 2015 Subject: [Histonet] HELP! Need some old fashioned histology advice Message-ID: <4DA4F062-023E-4C38-A8E3-B418812F34C0@gmail.com> First, the very best of holidays to everyone. Now for the histology part. Our lab's focus is on the early stages of Alzheimer's Disease in the Brainstem using celloidin processing & embedding for IHC staining. This year, our lab will be receiving 6 post-mortem whole human brains (1 every other month). After fixation, processing & celloidin embedding, the whole brain will be serially cut at 100um thick. Each brain section will be 5 inches x 4.5 inches in size. I will given 250 of these whole brain sections to stain for tau IHC...that's 1500 whole brain sections/year!!! 1) Does anyone have experience doing manual IHC staining of large free-floating brain sections? 2) What type of staining tools, dishes or other essential equipment can anyone recommend? 3) What's the most efficient way to stain 250 sections for batch IHC staining - such as transferring batch sections (maybe 5-10) from reagent to reagent? 4) What type of batch apparatus to use? As for the antibody & ABC steps, I was thinking of placing each section inside a large glass cigar tube (yep, people use large glass tubes with fitted cap to store cigars), with 5ml of antibody or ABC reagent & gently agitate on a shaker/rotator at room temp during the incubation. Does anyone have ideas on this? Please, any ideas, suggestions or recommendation anyone can provide will be most greatly appreciated. Best regards Maria Mejia UCSF Department of Neurology San Francisco, CA From jaylundgren <@t> gmail.com Sun Jan 4 11:58:38 2015 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Sun Jan 4 11:58:44 2015 Subject: [Histonet] HELP! Need some old fashioned histology advice In-Reply-To: <4DA4F062-023E-4C38-A8E3-B418812F34C0@gmail.com> References: <4DA4F062-023E-4C38-A8E3-B418812F34C0@gmail.com> Message-ID: I can help with the old fashioned advice: - 1 scant teaspoon simple syrup - 2 dashes Angostura Bitters, plus more to taste - 1 half dollar?sized slice orange peel, including pith - 2 ounces good-quality rye or bourbon - 1 maraschino cherry As for the Histology, is there any reason you cannot mount the sections onto glass slides? When I was working at Genentech they were cutting frozen sections through whole rabbits and mounting the sections on (giant) glass slides. I think that rolling the tissue up, inserting it, and then removing it from a glass tube would destroy the tissue. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Sat, Jan 3, 2015 at 8:01 PM, Maria Mejia wrote: > First, the very best of holidays to everyone. > > Now for the histology part. Our lab's focus is on the early stages of > Alzheimer's Disease in the Brainstem > using celloidin processing & embedding for IHC staining. This year, our > lab will be receiving 6 post-mortem > whole human brains (1 every other month). After fixation, processing & > celloidin embedding, the whole brain > will be serially cut at 100um thick. Each brain section will be 5 inches > x 4.5 inches in size. > > I will given 250 of these whole brain sections to stain for tau > IHC...that's 1500 whole brain sections/year!!! > 1) Does anyone have experience doing manual IHC staining of large > free-floating brain sections? > 2) What type of staining tools, dishes or other essential equipment can > anyone recommend? > 3) What's the most efficient way to stain 250 sections for batch IHC > staining - such as transferring batch > sections (maybe 5-10) from reagent to reagent? > 4) What type of batch apparatus to use? > > As for the antibody & ABC steps, I was thinking of placing each section > inside a large glass cigar tube > (yep, people use large glass tubes with fitted cap to store cigars), with > 5ml of antibody or ABC reagent & gently agitate on > a shaker/rotator at room temp during the incubation. Does anyone have > ideas on this? > > Please, any ideas, suggestions or recommendation anyone can provide will > be most greatly appreciated. > > Best regards > Maria Mejia > UCSF > Department of Neurology > San Francisco, CA > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pruegghm <@t> hotmail.com Sun Jan 4 21:03:10 2015 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Sun Jan 4 21:03:16 2015 Subject: [Histonet] HELP! Need some old fashioned histology advice In-Reply-To: References: <4DA4F062-023E-4C38-A8E3-B418812F34C0@gmail.com>, Message-ID: I have done something similar to this but I used tissue that was fixed but not processed and embedded, this is called enblock labeling, I infiltrated the fixed tissue with the IHC reagents, in a vial/tube, the blocking reagents, then the antibody, then the detection reagents and DAB, then dehydrated the tissue. I used vials or tubes on a platform shaker and would infiltrate reagents for days, then after it was done I infiltrated and embedded the tissue in glycol methacrylate (GMA) so that I could section it, it actually worked. The tissue was already IHc LABeled so all I did to the 5 micron sections after they were cut was a hematoxylin counterstain, this was mineralized bone so I had to embedd in something hard like GMA to section. Will you remove the Celloidin before trying to do the IHC staining? 100 micron sections might be easy to float/handle using a glass pipette for transferring. Sounds like an interesting project, good luck and feel free to ask for advise and keep us posted on your progress. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com > Date: Sun, 4 Jan 2015 11:58:38 -0600 > From: jaylundgren@gmail.com > To: mbmphoto@gmail.com > Subject: Re: [Histonet] HELP! Need some old fashioned histology advice > CC: histonet@lists.utsouthwestern.edu; mary.mejia@ucsf.edu > > I can help with the old fashioned advice: > > > - 1 scant teaspoon simple syrup > - 2 dashes Angostura Bitters, plus more to taste > - 1 half dollar?sized slice orange peel, including pith > - 2 ounces good-quality rye or bourbon > - 1 maraschino cherry > > As for the Histology, is there any reason you cannot mount the > sections onto glass slides? When I was working at Genentech they were > cutting frozen sections through whole rabbits and mounting the sections on > (giant) glass slides. I think that rolling the tissue up, inserting it, > and then removing it from a glass tube would destroy the tissue. > > Sincerely, > > Jay A. Lundgren, M.S., HTL > (ASCP) > > On Sat, Jan 3, 2015 at 8:01 PM, Maria Mejia wrote: > > > First, the very best of holidays to everyone. > > > > Now for the histology part. Our lab's focus is on the early stages of > > Alzheimer's Disease in the Brainstem > > using celloidin processing & embedding for IHC staining. This year, our > > lab will be receiving 6 post-mortem > > whole human brains (1 every other month). After fixation, processing & > > celloidin embedding, the whole brain > > will be serially cut at 100um thick. Each brain section will be 5 inches > > x 4.5 inches in size. > > > > I will given 250 of these whole brain sections to stain for tau > > IHC...that's 1500 whole brain sections/year!!! > > 1) Does anyone have experience doing manual IHC staining of large > > free-floating brain sections? > > 2) What type of staining tools, dishes or other essential equipment can > > anyone recommend? > > 3) What's the most efficient way to stain 250 sections for batch IHC > > staining - such as transferring batch > > sections (maybe 5-10) from reagent to reagent? > > 4) What type of batch apparatus to use? > > > > As for the antibody & ABC steps, I was thinking of placing each section > > inside a large glass cigar tube > > (yep, people use large glass tubes with fitted cap to store cigars), with > > 5ml of antibody or ABC reagent & gently agitate on > > a shaker/rotator at room temp during the incubation. Does anyone have > > ideas on this? > > > > Please, any ideas, suggestions or recommendation anyone can provide will > > be most greatly appreciated. > > > > Best regards > > Maria Mejia > > UCSF > > Department of Neurology > > San Francisco, CA > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cjohnson <@t> nmda.nmsu.edu Mon Jan 5 07:30:27 2015 From: cjohnson <@t> nmda.nmsu.edu (Johnson, Carole) Date: Mon Jan 5 07:30:37 2015 Subject: [Histonet] Formic acid disposal? Message-ID: Hello everyone and Happy New Year, Can anyone who is using formic acid in their lab tell me how they handle/dispose of it after use? I realize there is some variability by region, but any help would be appreciated. Thanks in advance. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From bcooper <@t> chla.usc.edu Mon Jan 5 10:28:03 2015 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Mon Jan 5 10:28:14 2015 Subject: [Histonet] RE: Formic acid disposal? In-Reply-To: References: Message-ID: Our waste disposal company has us combine our formic acid waste with our special stain/H&E stain waste. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Carole Sent: Monday, January 05, 2015 5:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formic acid disposal? Hello everyone and Happy New Year, Can anyone who is using formic acid in their lab tell me how they handle/dispose of it after use? I realize there is some variability by region, but any help would be appreciated. Thanks in advance. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From Ryan.Roy <@t> va.gov Mon Jan 5 11:23:43 2015 From: Ryan.Roy <@t> va.gov (Roy, Ryan) Date: Mon Jan 5 11:24:07 2015 Subject: [Histonet] RE: Formic acid disposal? In-Reply-To: References: Message-ID: <15F883394EAB744E99E1C7E1B98730490177061C2EB0@R04BYNMSGB1.r04.med.va.gov> Hello, We Label our waste containers with the type of waste, waste code, and date of collection. Then a waste company picks up as necessary. Ryan Roy HTL (ASCP) Manchester Veterans Affairs Medical Center Manchester New Hampshire Disclosure: The content of this email does not represent the views or opinons of the VA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Monday, January 05, 2015 11:28 AM To: Johnson, Carole; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] RE: Formic acid disposal? Our waste disposal company has us combine our formic acid waste with our special stain/H&E stain waste. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Carole Sent: Monday, January 05, 2015 5:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formic acid disposal? Hello everyone and Happy New Year, Can anyone who is using formic acid in their lab tell me how they handle/dispose of it after use? I realize there is some variability by region, but any help would be appreciated. Thanks in advance. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Mon Jan 5 12:11:42 2015 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Mon Jan 5 12:11:56 2015 Subject: [Histonet] RE: Formic acid disposal? In-Reply-To: References: , Message-ID: Our waste removal guys just had me put Formic acid in with all the other liquid wastes - alcohols, xylenes, wastes from special stains, etc. they disposed of it. Andi G. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Cooper, Brian [bcooper@chla.usc.edu] Sent: Monday, January 05, 2015 9:28 AM To: Johnson, Carole; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Formic acid disposal? Our waste disposal company has us combine our formic acid waste with our special stain/H&E stain waste. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Carole Sent: Monday, January 05, 2015 5:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Formic acid disposal? Hello everyone and Happy New Year, Can anyone who is using formic acid in their lab tell me how they handle/dispose of it after use? I realize there is some variability by region, but any help would be appreciated. Thanks in advance. Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Mon Jan 5 12:22:16 2015 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Mon Jan 5 12:22:26 2015 Subject: FW: [Histonet] RE: Formic acid disposal? In-Reply-To: <20150105181336.E024848F56@mmp.mokpo.ac.kr> References: <20150105181336.E024848F56@mmp.mokpo.ac.kr> Message-ID: I keep getting these emails. Since I can't read in whatever language this is I don't know what it says. They are asking for verification. What does this mean? In this day I don't just go putting my info out there to anyone. Is this person legit? Is anybody else getting these? Andi G. ________________________________________ From: moinet21@mokpo.ac.kr [moinet21@mokpo.ac.kr] Sent: Monday, January 05, 2015 11:13 AM To: Grantham, Andrea L - (algranth) Subject: RE: [Histonet] RE: Formic acid disposal? [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/logo.gif] ???????? ?? ?? ??? [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_left.gif] ?? ?????. ?? moinet21@mokpo.ac.kr ????? ??? ??? ??????? ???? ????? ?? ?????? ???? ????. ??? ??? ??? ????? ?? ? ??? ??? ????, ?? ???? ??? ??? ????, ??? ????? ??? ??? ? ????. ? ?? ?? ?? ???? ????,? ? ???? ??? ????? ????? [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/button.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_right.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_left.gif] ??: 30? ??? ????? ???? ?? ??, ??? ??? ?????, ? ?? ?? ?, ? ????? ??? ??? ????. [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_right.gif] ?Confirmation Service for Spam free [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_left.gif] Thank you for your email This is moinet21@mokpo.ac.kr Please complete the verification so that I can receive your email. ?After finishing this verification, you can be in my address book forever and won't need to do it again.? [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/button_1.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_right.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_left.gif] Warning: you must c_img to deliver the mail, or it will be droped in 30 days! [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_right.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/bottom.gif] From Ryan.Roy <@t> va.gov Mon Jan 5 12:25:02 2015 From: Ryan.Roy <@t> va.gov (Roy, Ryan) Date: Mon Jan 5 12:25:31 2015 Subject: [Histonet] RE: Formic acid disposal? In-Reply-To: References: <20150105181336.E024848F56@mmp.mokpo.ac.kr> Message-ID: <15F883394EAB744E99E1C7E1B98730490177061C2EB1@R04BYNMSGB1.r04.med.va.gov> I got this to.. Can an administrator explain or delete the source? thanks -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Monday, January 05, 2015 1:22 PM To: remove Subject: [EXTERNAL] FW: [Histonet] RE: Formic acid disposal? I keep getting these emails. Since I can't read in whatever language this is I don't know what it says. They are asking for verification. What does this mean? In this day I don't just go putting my info out there to anyone. Is this person legit? Is anybody else getting these? Andi G. ________________________________________ From: moinet21@mokpo.ac.kr [moinet21@mokpo.ac.kr] Sent: Monday, January 05, 2015 11:13 AM To: Grantham, Andrea L - (algranth) Subject: RE: [Histonet] RE: Formic acid disposal? [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/logo.gif] ???????? ?? ?? ??? [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_left.gif] ?? ?????. ?? moinet21@mokpo.ac.kr ????? ??? ??? ??????? ???? ????? ?? ?????? ???? ????. ??? ??? ??? ????? ?? ? ??? ??? ????, ?? ???? ??? ??? ????, ??? ????? ??? ??? ? ????. ? ?? ?? ?? ???? ????,? ? ???? ??? ????? ????? [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/button.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_right.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_left.gif] ??: 30? ??? ????? ???? ?? ??, ??? ??? ?????, ? ?? ?? ?, ? ????? ??? ??? ????. [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_right.gif] ?Confirmation Service for Spam free [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_left.gif] Thank you for your email This is moinet21@mokpo.ac.kr Please complete the verification so that I can receive your email. ?After finishing this verification, you can be in my address book forever and won't need to do it again.? [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/button_1.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_right.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_left.gif] Warning: you must c_img to deliver the mail, or it will be droped in 30 days! [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_right.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/bottom.gif] From hans <@t> histologistics.com Mon Jan 5 12:38:00 2015 From: hans <@t> histologistics.com (Hans B Snyder) Date: Mon Jan 5 12:38:12 2015 Subject: [Histonet] RE: Formic acid disposal? In-Reply-To: <15F883394EAB744E99E1C7E1B98730490177061C2EB1@R04BYNMSGB1.r04.med.va.gov> References: <20150105181336.E024848F56@mmp.mokpo.ac.kr> <15F883394EAB744E99E1C7E1B98730490177061C2EB1@R04BYNMSGB1.r04.med.va.gov> Message-ID: <521239A3-6770-4445-B290-13F0986813F3@histologistics.com> I believe it's spam or a scam. Histologistics Hans B Snyder 508.308.7800 Hans@histologistics.com > On Jan 5, 2015, at 13:25, Roy, Ryan wrote: > > I got this to.. > > Can an administrator explain or delete the source? > > thanks > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) > Sent: Monday, January 05, 2015 1:22 PM > To: remove > Subject: [EXTERNAL] FW: [Histonet] RE: Formic acid disposal? > > I keep getting these emails. Since I can't read in whatever language this is I don't know what it says. They are asking for verification. What does this mean? In this day I don't just go putting my info out there to anyone. Is this person legit? Is anybody else getting these? > > Andi G. > ________________________________________ > From: moinet21@mokpo.ac.kr [moinet21@mokpo.ac.kr] > Sent: Monday, January 05, 2015 11:13 AM > To: Grantham, Andrea L - (algranth) > Subject: RE: [Histonet] RE: Formic acid disposal? > > [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/logo.gif] > > > > > > ???????? ?? ?? ??? > [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_left.gif] > > ?? ?????. > > ?? moinet21@mokpo.ac.kr ????? ??? ??? > > > > ??????? ???? ????? ?? ?????? ???? ????. > > ??? ??? ??? ????? ?? ? ??? ??? ????, ?? ???? ??? ??? ????, ??? ????? ??? ??? ? ????. > > ? ?? ?? ?? ???? ????,? ? ???? ??? ????? ????? > > > [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/button.gif] > > > > [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_right.gif] > > > > > > > [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_left.gif] > > ??: 30? ??? ????? ???? ?? ??, ??? ??? ?????, > ? ?? ?? ?, ? ????? ??? ??? ????. > > > [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_right.gif] > > > > > > > > > > ?Confirmation Service for Spam free > [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_left.gif] > > Thank you for your email > > This is moinet21@mokpo.ac.kr > > > > Please complete the verification so that I can receive your email. > > > > ?After finishing this verification, > > you can be in my address book forever and won't need to do it again.? > > > [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/button_1.gif] > > > > [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_right.gif] > > > > > > > > > > [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_left.gif] > > Warning: you must c_img to deliver the mail, or it will be droped in 30 days! > > > [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_right.gif] > > > > > > > [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/bottom.gif] > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From James.E.Nutter <@t> questdiagnostics.com Mon Jan 5 12:47:20 2015 From: James.E.Nutter <@t> questdiagnostics.com (Nutter, James E) Date: Mon Jan 5 12:49:10 2015 Subject: FW: [Histonet] RE: Formic acid disposal? Message-ID: This is the translation. Mokpo National University Webmail has implemented an authentication service to fight spam. To send a letter to me therefore become certified for the first time once completed, you must have in my address book, you can send mail to me normally. Once in my address book since this authentication, mail is sent then the authentication process is skipped WARNING: If you do not send the certificate number 30 days, archived mail this erases, After the email confirmation, please just enter your authorization number. James E. Nutter Jr. BS, HT & QIHC(ASCP) Quest Diagnostics Nichols Institute | Histology| 14225 Newbrook Dr.| Chantilly Va. USA | phone 703.802.6900 x65782| fax 703.802.7191| | James.E.Nutter@QuestDiagnostics.com | www.NicholsInstitute.com ______________________________________________________________________ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. From Ryan.Roy <@t> va.gov Tue Jan 6 09:14:29 2015 From: Ryan.Roy <@t> va.gov (Roy, Ryan) Date: Tue Jan 6 09:15:35 2015 Subject: [Histonet] RE: Formic acid disposal? In-Reply-To: References: <20150105181336.E024848F56@mmp.mokpo.ac.kr> <15F883394EAB744E99E1C7E1B98730490177061C2EB1@R04BYNMSGB1.r04.med.va.gov> Message-ID: <15F883394EAB744E99E1C7E1B98730490177061C2EB4@R04BYNMSGB1.r04.med.va.gov> Oh ok that makes sense. Thanks -----Original Message----- From: Smith, Allen [mailto:asmith@barry.edu] Sent: Monday, January 05, 2015 4:59 PM To: Roy, Ryan Cc: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] RE: [Histonet] RE: Formic acid disposal? The language is Korean. The message originates from Mokpo University in South Korea. It warns that your message has been blocked. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roy, Ryan Sent: Monday, January 05, 2015 1:25 PM To: 'Grantham, Andrea L - (algranth)'; remove Subject: RE: [Histonet] RE: Formic acid disposal? I got this to.. Can an administrator explain or delete the source? thanks -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Grantham, Andrea L - (algranth) Sent: Monday, January 05, 2015 1:22 PM To: remove Subject: [EXTERNAL] FW: [Histonet] RE: Formic acid disposal? I keep getting these emails. Since I can't read in whatever language this is I don't know what it says. They are asking for verification. What does this mean? In this day I don't just go putting my info out there to anyone. Is this person legit? Is anybody else getting these? Andi G. ________________________________________ From: moinet21@mokpo.ac.kr [moinet21@mokpo.ac.kr] Sent: Monday, January 05, 2015 11:13 AM To: Grantham, Andrea L - (algranth) Subject: RE: [Histonet] RE: Formic acid disposal? [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/logo.gif] ???????? ?? ?? ??? [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_left.gif] ?? ?????. ?? moinet21@mokpo.ac.kr ????? ??? ??? ??????? ???? ????? ?? ?????? ???? ????. ??? ??? ??? ????? ?? ? ??? ??? ????, ?? ???? ??? ??? ????, ??? ????? ??? ??? ? ????. ? ?? ?? ?? ???? ????,? ? ???? ??? ????? ????? [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/button.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_right.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_left.gif] ??: 30? ??? ????? ???? ?? ??, ??? ??? ?????, ? ?? ?? ?, ? ????? ??? ??? ????. [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_right.gif] ?Confirmation Service for Spam free [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_left.gif] Thank you for your email This is moinet21@mokpo.ac.kr Please complete the verification so that I can receive your email. ?After finishing this verification, you can be in my address book forever and won't need to do it again.? [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/button_1.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/msg_box_right.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_left.gif] Warning: you must c_img to deliver the mail, or it will be droped in 30 days! [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/w_msg_box_right.gif] [http://mmp.mokpo.ac.kr:8886/mmpmgr/images/c_img/bottom.gif] From jvickroy <@t> SpringfieldClinic.com Tue Jan 6 09:16:39 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Tue Jan 6 09:16:50 2015 Subject: [Histonet] Cyberlab pathology manual and Dragon Message-ID: <9B1A1501A800064397369BD8072E6BCA96A301@E2K10DB.springfieldclinic.com> I have recently moved to a new organization and am setting up a new surgical pathology lab. The lab LIS system is Cyberlab and I am told that they have a AP module. In the past I have worked with Cerner Classic and CoPath Plus at a hospital but this lab will be a much smaller set-up. Obviously the organization would like us to use the AP module since the interfacing issues would be markedly reduced. I am set for a demonstration of the pathology module later this week but wondered if anyone could share their experiences with Cyperlab and specifically their pathology module. We will mainly be handling GI biopsies and skin biopsies. The clinic also has Dragon being used in other areas. One of my big questions is whether we will be able to use voice recognition in grossing and for result reporting by the pathologist. If you can share any information I would be very appreciative. Thanks Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From christiecgowan <@t> dermatology.med.ufl.edu Mon Jan 5 10:24:53 2015 From: christiecgowan <@t> dermatology.med.ufl.edu (Gowan,Christie C) Date: Tue Jan 6 11:42:35 2015 Subject: [Histonet] Cost per block on the Sakura Xpress Message-ID: Dear Histonetters, Does anyone have an algorithm for cost per block on the Sakura Xpress? I would need it for biopsy specimens only. I inherited one of these machines and before I put it into use, I want to know what it will cost me and yes I have reached out to Sakura (Florida) but not all my questions have been answered as of yet. Many thanks! Christie Gowan HT (ASCP) University of Florida Department of Dermatology 4037 NW 86th Terrace Gainesville, FL 32606 Phone: 352 594-1529 From tpassaro <@t> cellnetix.com Mon Jan 5 17:22:32 2015 From: tpassaro <@t> cellnetix.com (Tiffany Passaro) Date: Tue Jan 6 11:42:37 2015 Subject: [Histonet] PASD muscle stains Message-ID: Greetings, I am looking for fixatives that others are using in their labs for the PASD stain on fresh frozen muscle tissue. Currently we are fixing in 10% NBF. Thanks in advance for any info on this. Tiffany DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From Mary.Mejia <@t> ucsf.edu Mon Jan 5 22:27:21 2015 From: Mary.Mejia <@t> ucsf.edu (Mejia, Mary) Date: Tue Jan 6 11:42:37 2015 Subject: [Histonet] HELP! Need some old fashioned histology advice In-Reply-To: References: <4DA4F062-023E-4C38-A8E3-B418812F34C0@gmail.com>, Message-ID: <265E51BF3D1E7248969B92BD46E1F6AA527587@ex03.net.ucsf.edu> OK Jay, I'm going to have to make this drink of your - thanks for the recipe. How thick were these whole rabbit mounted sections? Were the giant glass slides gelatin subbed? I was thinking of mounting a couple of these large 100um brain sections & see how good penetration is for the antibodies. I was also thinking of adding 1% DMSO in all the washes - don't about the antibodies though. Any thoughts on the latter? Any other ideas? Best Maria ________________________________ From: Jay Lundgren [jaylundgren@gmail.com] Sent: Sunday, January 04, 2015 9:58 AM To: Maria Mejia Cc: histonet; Mejia, Mary Subject: Re: [Histonet] HELP! Need some old fashioned histology advice I can help with the old fashioned advice: * 1 scant teaspoon simple syrup * 2 dashes Angostura Bitters, plus more to taste * 1 half dollar?sized slice orange peel, including pith * 2 ounces good-quality rye or bourbon * 1 maraschino cherry As for the Histology, is there any reason you cannot mount the sections onto glass slides? When I was working at Genentech they were cutting frozen sections through whole rabbits and mounting the sections on (giant) glass slides. I think that rolling the tissue up, inserting it, and then removing it from a glass tube would destroy the tissue. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Sat, Jan 3, 2015 at 8:01 PM, Maria Mejia > wrote: First, the very best of holidays to everyone. Now for the histology part. Our lab's focus is on the early stages of Alzheimer's Disease in the Brainstem using celloidin processing & embedding for IHC staining. This year, our lab will be receiving 6 post-mortem whole human brains (1 every other month). After fixation, processing & celloidin embedding, the whole brain will be serially cut at 100um thick. Each brain section will be 5 inches x 4.5 inches in size. I will given 250 of these whole brain sections to stain for tau IHC...that's 1500 whole brain sections/year!!! 1) Does anyone have experience doing manual IHC staining of large free-floating brain sections? 2) What type of staining tools, dishes or other essential equipment can anyone recommend? 3) What's the most efficient way to stain 250 sections for batch IHC staining - such as transferring batch sections (maybe 5-10) from reagent to reagent? 4) What type of batch apparatus to use? As for the antibody & ABC steps, I was thinking of placing each section inside a large glass cigar tube (yep, people use large glass tubes with fitted cap to store cigars), with 5ml of antibody or ABC reagent & gently agitate on a shaker/rotator at room temp during the incubation. Does anyone have ideas on this? Please, any ideas, suggestions or recommendation anyone can provide will be most greatly appreciated. Best regards Maria Mejia UCSF Department of Neurology San Francisco, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Mary.Mejia <@t> ucsf.edu Mon Jan 5 22:38:51 2015 From: Mary.Mejia <@t> ucsf.edu (Mejia, Mary) Date: Tue Jan 6 11:42:39 2015 Subject: [Histonet] HELP! Need some old fashioned histology advice In-Reply-To: References: <4DA4F062-023E-4C38-A8E3-B418812F34C0@gmail.com>, , Message-ID: <265E51BF3D1E7248969B92BD46E1F6AA527597@ex03.net.ucsf.edu> Hello Patsy, Thank you very much for responding! Yes & of course, I'll be removing the celloidin from each section - we normally do this on smaller human whole brainstem sections using ether/100% EA 1:1 - 3x - 3 minutes each with agitation. The latter type of sections are very easy to work with, however these future big boys I'll be getting is another thing. Our lab is currently alcohol processing a human whole brain & after the last 95% EA - it will be embedded in 2% celloidin & placed inside a very large desiccator under 20 psi pressure. This part like every step will take some period of time, I need to test several different IHC methods & hope one will actually work. If you have any further ideas or thoughts on this subject - shoot me an email. Thank you again for responding. Maria ________________________________ From: Patsy Ruegg [pruegghm@hotmail.com] Sent: Sunday, January 04, 2015 7:03 PM To: Jay Lundgren; Maria Mejia Cc: Histonet@Lists. Edu; Mejia, Mary Subject: RE: [Histonet] HELP! Need some old fashioned histology advice I have done something similar to this but I used tissue that was fixed but not processed and embedded, this is called enblock labeling, I infiltrated the fixed tissue with the IHC reagents, in a vial/tube, the blocking reagents, then the antibody, then the detection reagents and DAB, then dehydrated the tissue. I used vials or tubes on a platform shaker and would infiltrate reagents for days, then after it was done I infiltrated and embedded the tissue in glycol methacrylate (GMA) so that I could section it, it actually worked. The tissue was already IHc LABeled so all I did to the 5 micron sections after they were cut was a hematoxylin counterstain, this was mineralized bone so I had to embedd in something hard like GMA to section. Will you remove the Celloidin before trying to do the IHC staining? 100 micron sections might be easy to float/handle using a glass pipette for transferring. Sounds like an interesting project, good luck and feel free to ask for advise and keep us posted on your progress. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com > Date: Sun, 4 Jan 2015 11:58:38 -0600 > From: jaylundgren@gmail.com > To: mbmphoto@gmail.com > Subject: Re: [Histonet] HELP! Need some old fashioned histology advice > CC: histonet@lists.utsouthwestern.edu; mary.mejia@ucsf.edu > > I can help with the old fashioned advice: > > > - 1 scant teaspoon simple syrup > - 2 dashes Angostura Bitters, plus more to taste > - 1 half dollar?sized slice orange peel, including pith > - 2 ounces good-quality rye or bourbon > - 1 maraschino cherry > > As for the Histology, is there any reason you cannot mount the > sections onto glass slides? When I was working at Genentech they were > cutting frozen sections through whole rabbits and mounting the sections on > (giant) glass slides. I think that rolling the tissue up, inserting it, > and then removing it from a glass tube would destroy the tissue. > > Sincerely, > > Jay A. Lundgren, M.S., HTL > (ASCP) > > On Sat, Jan 3, 2015 at 8:01 PM, Maria Mejia wrote: > > > First, the very best of holidays to everyone. > > > > Now for the histology part. Our lab's focus is on the early stages of > > Alzheimer's Disease in the Brainstem > > using celloidin processing & embedding for IHC staining. This year, our > > lab will be receiving 6 post-mortem > > whole human brains (1 every other month). After fixation, processing & > > celloidin embedding, the whole brain > > will be serially cut at 100um thick. Each brain section will be 5 inches > > x 4.5 inches in size. > > > > I will given 250 of these whole brain sections to stain for tau > > IHC...that's 1500 whole brain sections/year!!! > > 1) Does anyone have experience doing manual IHC staining of large > > free-floating brain sections? > > 2) What type of staining tools, dishes or other essential equipment can > > anyone recommend? > > 3) What's the most efficient way to stain 250 sections for batch IHC > > staining - such as transferring batch > > sections (maybe 5-10) from reagent to reagent? > > 4) What type of batch apparatus to use? > > > > As for the antibody & ABC steps, I was thinking of placing each section > > inside a large glass cigar tube > > (yep, people use large glass tubes with fitted cap to store cigars), with > > 5ml of antibody or ABC reagent & gently agitate on > > a shaker/rotator at room temp during the incubation. Does anyone have > > ideas on this? > > > > Please, any ideas, suggestions or recommendation anyone can provide will > > be most greatly appreciated. > > > > Best regards > > Maria Mejia > > UCSF > > Department of Neurology > > San Francisco, CA > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lori <@t> cgenetool.com Tue Jan 6 10:59:01 2015 From: lori <@t> cgenetool.com (Lori McDonald) Date: Tue Jan 6 11:42:39 2015 Subject: [Histonet] Happy New Year! Message-ID: <0.1.BF.4A4.1D029D21201F240.0@drone146.ral.icpbounce.com> Wish you a Prosperous and Exciting..... 2015 We look foward to working with you this year!! NEW ARRIVAL: [1]BD FACSCanto II With Fluidics Cart Manufactured in 2008 Dual Laser, 7 color set up D NEW ARRIVAL: [2]Biacore T-200 - Upgraded from a Biacore T-100 [3]ABI 3500- 8 Capillaries NEW, NEVER USED!! Services Offered: Repairs Service Contracts Upgrades WE BUY SURPLUS We specialize in the following Instruments: DNA Sequencers Real-Time PCRS Biacore Systems Plate Readers Flow Cytometers Beckman Centrifuges Rotors- Beckman HPLC/FPLC Agilent Bioanalyzers DNA Synthesizers Imaging Systems Axon Scanners Parts Testimonial Repair service for GE Storm Imager 865 The storm arrived about an hour ago. We set it up and it is working fine. In fact, the images obtained are stronger than we have observed over the last couple of years. I hope we don?t need your services for a long time, but I will tell my colleagues that Certified Genetool was a great company. George Rutgers UniversityC. pany. George George C*** Rutgers University Lori McDonald Certified GeneTool, Inc. 7074 Commerce Circle, Ste. B Pleasanton, CA 94588 Phone: 925-737-0800 Email: [4]lori@cgenetool.com Website: [5]www.cgenetool.com References 1. mailto:lori@cgenetool.com?subject=FACSCanto%20II%20Inquiry 2. mailto:lori@cgenetool.com?subject=Biacore%20T200%20Inquiry 3. mailto:lori@cgenetool.com?subject=ABI%203500%20Inquiry 4. mailto:lori@cgenetool 5. http://www.cgenetool.com/ This message was sent to histonet@lists.utsouthwestern.edu from: Lori McDonald | lori@cgenetool.com | brian qian | 7074 Commerce Circle | Pleasanton, CA 94588 Unsubscribe: http://app.icontact.com/icp/mmail-mprofile.pl?r=47317229&l=9237&s=P71X&m=330890&c=1188219 From jvickroy <@t> SpringfieldClinic.com Mon Jan 5 08:41:54 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Tue Jan 6 11:44:27 2015 Subject: [Histonet] VIP 6 processing baskets Message-ID: <9B1A1501A800064397369BD8072E6BCA96A0D0@E2K10DB.springfieldclinic.com> Does anyone know if the VIP 6 cassette baskets fit other vendor embedding centers? Spec sheets have a lot of measurements but practical experience is the best guide. We are considering a Thermofisher embedding center or a Leica Embedding Center but most likely we will process with a VIP 6 so I want to make sure the baskets will fit. I know that VIP has two sizes of baskets and I am most concerned about the 150 cassette basket. Thanks Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From lcolbert <@t> pathmdlabs.com Tue Jan 6 12:24:13 2015 From: lcolbert <@t> pathmdlabs.com (Laurie Colbert) Date: Tue Jan 6 12:24:24 2015 Subject: [Histonet] Full Time HT position in Los Angeles, CA Message-ID: <12ECD7346266D74691EC2BFC75285E45473CB6EA@BFL323E10.pathmdlabs.local> PATH MD, a state-of-the-art reference pathology laboratory located in Los Angeles, CA, is looking for two full time histotechnicians to start sometime in the first quarter of 2015. Applicant must be a motivated team player and have at least one year of experience and be ASCP registered. Proficiency in embedding, cutting and special stains is required. PATH MD offers full medical, dental, and vision benefits as well as a 401(k) plan. Interested applicants may send their resumes to: lcolbert@pathmdlabs.com Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From bburnett <@t> CapeCodHealth.org Tue Jan 6 12:38:41 2015 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Tue Jan 6 12:38:47 2015 Subject: [Histonet] HER2 IHC controls Message-ID: Are any of you making your own HER2 IHC control slides from patient tissue? ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From pruegghm <@t> hotmail.com Tue Jan 6 13:13:15 2015 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Tue Jan 6 13:13:25 2015 Subject: [Histonet] cutting honey bees In-Reply-To: References: , Message-ID: for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com > From: rjr6@psu.edu > To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu > Date: Sat, 3 Jan 2015 23:15:33 +0000 > Subject: RE: [Histonet] cutting honey bees > CC: > > I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. > Roberta Horner > Penn State University > Animal Diagnostic Lab > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classicdoc@gmail.com] > Sent: Saturday, January 03, 2015 6:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cutting honey bees > > Has anyone had experience embedding and cutting honey bees. I am sure > there are some issues with the harder exoskeleton. Would that have to > be dissected away first. I am considering helping a student with a > science fair project on bees. > > Douglas Gregg > Veterianary pathologist > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Tue Jan 6 13:20:01 2015 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Tue Jan 6 13:20:36 2015 Subject: [Histonet] PASD muscle stains In-Reply-To: References: Message-ID: <1030697348.15323377.1420572001302.JavaMail.zimbra@comcast.net> Tiffany, Have used 10%NBF on muscles but also alcoholic fixatives -alcoholic formalin or absolute- just always preferred 10%NBF since it gave the morphology and counterstaining I wanted.? diastase in a 6.0pH buffer (don't heat above 40 degrees if trying to speed up heating-kill the diastase) and always stayed away from di water on frozen sections.? di water too variable and fickle. ? Ray Koelling Lake Forest Park ----- Original Message ----- From: "Tiffany Passaro" To: "histonet@lists.utsouthwestern.edu" Sent: Monday, January 5, 2015 3:22:32 PM Subject: [Histonet] PASD muscle stains Greetings, ?? ? ? ? ? ? ? ?I am looking for fixatives that others are using in their labs for the PASD stain on fresh frozen muscle tissue. Currently we are fixing in 10% NBF. Thanks in advance for any info on this. Tiffany DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjr6 <@t> psu.edu Tue Jan 6 14:54:10 2015 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Tue Jan 6 14:54:20 2015 Subject: And other crazy stuff. RE: [Histonet] cutting honey bees In-Reply-To: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> Message-ID: The oddest things I cut were the honey bee and yellow jacket stingers. I've done plant stamen, reptiles, fish and I believe another insect. I usually tell the students that are working on a research project to give me a sample they don't care about so I can see if I can do what they want. But I had oddities that I didn't have to section like during hunting season a hunter killed a deer and there was a mass on the trachea that he wanted tested to make sure the deer was okay to eat. I got the sample and when I tried to gross it I found a very hard shiny silver object. I told the pathologist whose case it was that the mass was from a bullet did he still want histo done. No. The other interesting one was the egg shell. The conversation went something like this. Pathologist: Can you section this egg shell Me: No it's too hard. P: Can't you decal it M: That's not going to work. P: Did you try. M: No P: Don't you think you should try first. M: Okay fine but it is no going to work. Put a piece of eggshell (made of calcium) into some decal solution (that removes calcium) and watch the egg shell bubble and disappear. I did get to tell the pathologist "I told you so" Roberta Horner Penn State University Animal Diagnostic Lab -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken@ucsf.edu] Sent: Tuesday, January 06, 2015 2:24 PM To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: And other crazy stuff. RE: [Histonet] cutting honey bees You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates.... Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com > From: rjr6@psu.edu > To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu > Date: Sat, 3 Jan 2015 23:15:33 +0000 > Subject: RE: [Histonet] cutting honey bees > CC: > > I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. > Roberta Horner > Penn State University > Animal Diagnostic Lab > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg > [classicdoc@gmail.com] > Sent: Saturday, January 03, 2015 6:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cutting honey bees > > Has anyone had experience embedding and cutting honey bees. I am sure > there are some issues with the harder exoskeleton. Would that have to > be dissected away first. I am considering helping a student with a > science fair project on bees. > > Douglas Gregg > Veterianary pathologist > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Jan 6 14:59:34 2015 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue Jan 6 15:02:09 2015 Subject: [Histonet] RE: Cost per block on the Sakura Xpress In-Reply-To: References: Message-ID: <3B2CD438E1628A41BD687E98B963B7812CA46E55@EMBX-CLFT4.cdc.gov> I have seriously Important Info how to keep your costs as low as possible. We were the first lab to implement this plan and it works perfectly for us! Give me a call for details. Jeanine Sanders CDC Atlanta 404-639-3590 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gowan,Christie C Sent: Tuesday, December 30, 2014 3:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost per block on the Sakura Xpress Dear Histonetters, Does anyone have an algorithm for cost per block on the Sakura Xpress? I would need it for biopsy specimens only. I inherited one of these machines and before I put it into use, I want to know what it will cost me and yes I have reached out to Sakura (Florida) but no responses as of yet. Many thanks! Christie Gowan HT (ASCP) University of Florida Department of Dermatology 4037 NW 86th Terrace Gainesville, FL 32606 Phone: 352 594-1529 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cmconway <@t> usgs.gov Tue Jan 6 15:24:28 2015 From: cmconway <@t> usgs.gov (Conway, Carla) Date: Tue Jan 6 15:24:50 2015 Subject: [Histonet] Automatic H & E slide stainer recommendations Message-ID: Hello colleagues, I would appreciate any recommendations for an automatic slide stainer. It will primarily be used for H & E staining, not IHC. Thanks in advance, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway@usgs.gov From LSebree <@t> uwhealth.org Tue Jan 6 16:10:12 2015 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Tue Jan 6 16:10:23 2015 Subject: [Histonet] RE: HER2 IHC controls In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF0FE5A6@UWHC-MBX12.uwhis.hosp.wisc.edu> Yes, 3 different antibody expressions per block. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Tuesday, January 06, 2015 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HER2 IHC controls Are any of you making your own HER2 IHC control slides from patient tissue? ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jrsmallwood <@t> bell.net Tue Jan 6 16:18:57 2015 From: jrsmallwood <@t> bell.net (John Smallwood) Date: Tue Jan 6 16:20:18 2015 Subject: [Histonet] Celestin Blue B Message-ID: Hello Histologists: Is there anywhere to get this ? Celestin Blue B C.I. 51050 From jaylundgren <@t> gmail.com Tue Jan 6 16:26:58 2015 From: jaylundgren <@t> gmail.com (Jay Lundgren) Date: Tue Jan 6 16:27:05 2015 Subject: [Histonet] HELP! Need some old fashioned histology advice In-Reply-To: <265E51BF3D1E7248969B92BD46E1F6AA527597@ex03.net.ucsf.edu> References: <4DA4F062-023E-4C38-A8E3-B418812F34C0@gmail.com> <265E51BF3D1E7248969B92BD46E1F6AA527597@ex03.net.ucsf.edu> Message-ID: I don't know if the slides were subbed or not, but they looked improvised. The point is, at some point the sections are going to have to be mounted for visualisation, right? Someone or something is going to look at the section under magnification? If you mount them on glass *before* staining, the whole problem of batching becomes one of improvising a giant stain rack and giant staining line. Don't forget, you need giant cover slips, giant slide folders, and giant postdocs. I know that lab glassware is custom ordered every day. Sounds expensive and time consuming to set up, but it beats fiddling around with free floating tissue anyday, IMHO. I don't know what the Ab penetration is going to be like on a 100um section, because they weren't using IHC at Genentech, they were doing in vitro DNA hybridization on the slides. I'm assuming the section thickness is necessary because the PI wants to follow axonal pathways and see the patterns of staining? Sounds like a fun project. If you need more help later you can PM me. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Jan 5, 2015 at 10:38 PM, Mejia, Mary wrote: > Hello Patsy, > > Thank you very much for responding! Yes & of course, I'll be removing > the celloidin from each section - we normally > do this on smaller human whole brainstem sections using ether/100% EA 1:1 > - 3x - 3 minutes each with agitation. The > latter type of sections are very easy to work with, however these future > big boys I'll be getting is another thing. > > Our lab is currently alcohol processing a human whole brain & after the > last 95% EA - it will be embedded in 2% celloidin > & placed inside a very large desiccator under 20 psi pressure. This part > like every step will take some period of time, > I need to test several different IHC methods & hope one will actually work. > > If you have any further ideas or thoughts on this subject - shoot me an > email. Thank you again for responding. > > Maria > ------------------------------ > *From:* Patsy Ruegg [pruegghm@hotmail.com] > *Sent:* Sunday, January 04, 2015 7:03 PM > *To:* Jay Lundgren; Maria Mejia > *Cc:* Histonet@Lists. Edu; Mejia, Mary > *Subject:* RE: [Histonet] HELP! Need some old fashioned histology advice > > I have done something similar to this but I used tissue that was fixed > but not processed and embedded, this is called enblock labeling, I > infiltrated the fixed tissue with the IHC reagents, in a vial/tube, the > blocking reagents, then the antibody, then the detection reagents and DAB, > then dehydrated the tissue. I used vials or tubes on a platform shaker and > would infiltrate reagents for days, then after it was done I infiltrated > and embedded the tissue in glycol methacrylate (GMA) so that I could > section it, it actually worked. The tissue was already IHc LABeled so all > I did to the 5 micron sections after they were cut was a hematoxylin > counterstain, this was mineralized bone so I had to embedd in something > hard like GMA to section. > > Will you remove the Celloidin before trying to do the IHC staining? 100 > micron sections might be easy to float/handle using a glass pipette for > transferring. Sounds like an interesting project, good luck and feel free > to ask for advise and keep us posted on your progress. > > Cheers, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > pruegghm@hotmail.com > > > > > Date: Sun, 4 Jan 2015 11:58:38 -0600 > > From: jaylundgren@gmail.com > > To: mbmphoto@gmail.com > > Subject: Re: [Histonet] HELP! Need some old fashioned histology advice > > CC: histonet@lists.utsouthwestern.edu; mary.mejia@ucsf.edu > > > > I can help with the old fashioned advice: > > > > > > - 1 scant teaspoon simple syrup > > - 2 dashes Angostura Bitters, plus more to taste > > - 1 half dollar?sized slice orange peel, including pith > > - 2 ounces good-quality rye or bourbon > > - 1 maraschino cherry > > > > As for the Histology, is there any reason you cannot mount the > > sections onto glass slides? When I was working at Genentech they were > > cutting frozen sections through whole rabbits and mounting the sections > on > > (giant) glass slides. I think that rolling the tissue up, inserting it, > > and then removing it from a glass tube would destroy the tissue. > > > > Sincerely, > > > > Jay A. Lundgren, M.S., HTL > > (ASCP) > > > > On Sat, Jan 3, 2015 at 8:01 PM, Maria Mejia wrote: > > > > > First, the very best of holidays to everyone. > > > > > > Now for the histology part. Our lab's focus is on the early stages of > > > Alzheimer's Disease in the Brainstem > > > using celloidin processing & embedding for IHC staining. This year, our > > > lab will be receiving 6 post-mortem > > > whole human brains (1 every other month). After fixation, processing & > > > celloidin embedding, the whole brain > > > will be serially cut at 100um thick. Each brain section will be 5 > inches > > > x 4.5 inches in size. > > > > > > I will given 250 of these whole brain sections to stain for tau > > > IHC...that's 1500 whole brain sections/year!!! > > > 1) Does anyone have experience doing manual IHC staining of large > > > free-floating brain sections? > > > 2) What type of staining tools, dishes or other essential equipment can > > > anyone recommend? > > > 3) What's the most efficient way to stain 250 sections for batch IHC > > > staining - such as transferring batch > > > sections (maybe 5-10) from reagent to reagent? > > > 4) What type of batch apparatus to use? > > > > > > As for the antibody & ABC steps, I was thinking of placing each section > > > inside a large glass cigar tube > > > (yep, people use large glass tubes with fitted cap to store cigars), > with > > > 5ml of antibody or ABC reagent & gently agitate on > > > a shaker/rotator at room temp during the incubation. Does anyone have > > > ideas on this? > > > > > > Please, any ideas, suggestions or recommendation anyone can provide > will > > > be most greatly appreciated. > > > > > > Best regards > > > Maria Mejia > > > UCSF > > > Department of Neurology > > > San Francisco, CA > > > > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pruegghm <@t> hotmail.com Tue Jan 6 16:38:30 2015 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Tue Jan 6 16:38:36 2015 Subject: And other crazy stuff. RE: [Histonet] cutting honey bees In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu>, Message-ID: The coolest thing I cut was 600 yo deer bone. One of our pathologist did archeology as a hobby and wanted me to section it, it was petrified as hard as a rock. We tried everything to soften it to no avail. We ended up cutting with a diamond wire lapidary saw without embedding it in anything. Could make sections as thin as 30 microns. Had a heck of a time trying to get sections to adhere to a glass slide and the sections would not take any stain. We did end up looking at it with a fluorescent scope. We could see rings like a tree of bone turn over. This is how tetracycline labeling of bone first came about. Someone way back stuck a piece of dinosaur bone under UV light and saw rings, apparently the animals eat moldy grain ( tet is made from mold) and it deposits where ever new bone is being laid down, it also happens to fluoresce. I spent 25 years in a metabolic bone disease lab, we treated the patient with a course of tetracycline then waited for a period of time, I think it was 10-14 days, then the patient took another dose of tet and then within a day or 3 we biopsied their bone usually from the illiac crest fixed it in methanol because the tetracycline was water soluble then processed it into GMA plastic without decal. Unstained 5 micron sections cut with a tungsten carbide blade were reviewed with a fluorescent scope revealing the two labels of tet, since we knew the time between doses we could measure the area between the two labels and report them out as bone growth in mm per day. People with severe lack of bone turn over would just have one single label meaning they were not making much bone. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com > From: rjr6@psu.edu > To: Timothy.Morken@ucsf.edu; pruegghm@hotmail.com; classicdoc@gmail.com; histonet@lists.utsouthwestern.edu > Subject: RE: And other crazy stuff. RE: [Histonet] cutting honey bees > Date: Tue, 6 Jan 2015 20:54:10 +0000 > > The oddest things I cut were the honey bee and yellow jacket stingers. I've done plant stamen, reptiles, fish and I believe another insect. I usually tell the students that are working on a research project to give me a sample they don't care about so I can see if I can do what they want. > > But I had oddities that I didn't have to section like during hunting season a hunter killed a deer and there was a mass on the trachea that he wanted tested to make sure the deer was okay to eat. I got the sample and when I tried to gross it I found a very hard shiny silver object. I told the pathologist whose case it was that the mass was from a bullet did he still want histo done. No. > > The other interesting one was the egg shell. > The conversation went something like this. > Pathologist: Can you section this egg shell > Me: No it's too hard. > P: Can't you decal it > M: That's not going to work. > P: Did you try. > M: No > P: Don't you think you should try first. > M: Okay fine but it is no going to work. > > Put a piece of eggshell (made of calcium) into some decal solution (that removes calcium) and watch the egg shell bubble and disappear. I did get to tell the pathologist "I told you so" > > Roberta Horner > Penn State University > Animal Diagnostic Lab > > -----Original Message----- > From: Morken, Timothy [mailto:Timothy.Morken@ucsf.edu] > Sent: Tuesday, January 06, 2015 2:24 PM > To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > Subject: And other crazy stuff. RE: [Histonet] cutting honey bees > > You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? > > For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. > > > Open the floodgates.... > > Tim Morken > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg > Sent: Tuesday, January 06, 2015 11:13 AM > To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > Subject: RE: [Histonet] cutting honey bees > > for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. > > Cheers, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > pruegghm@hotmail.com > > > > > From: rjr6@psu.edu > > To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu > > Date: Sat, 3 Jan 2015 23:15:33 +0000 > > Subject: RE: [Histonet] cutting honey bees > > CC: > > > > I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. > > Roberta Horner > > Penn State University > > Animal Diagnostic Lab > > ________________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu > > [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg > > [classicdoc@gmail.com] > > Sent: Saturday, January 03, 2015 6:08 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] cutting honey bees > > > > Has anyone had experience embedding and cutting honey bees. I am sure > > there are some issues with the harder exoskeleton. Would that have to > > be dissected away first. I am considering helping a student with a > > science fair project on bees. > > > > Douglas Gregg > > Veterianary pathologist > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mills <@t> 3scan.com Tue Jan 6 18:38:15 2015 From: mills <@t> 3scan.com (Caroline Miller) Date: Tue Jan 6 18:38:29 2015 Subject: And other crazy stuff. RE: [Histonet] cutting honey bees In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> Message-ID: When I worked in a research core (which was only last week, but I just changed jobs). I have processed and cut (with varying degrees of success) fake meat samples for a company. They were mainly made of grains and mushed veggies. The problem was the samples were not consistent and there was no room (time or money) for honing the protocol for each of the many samples he sent me, so he got what I could cut. He always seemed pleased, but I couldn't see much in the samples. He was very secretive about it all, so I could never quite understand what they were doing t all for!!! C Sent from my iPhone > On Jan 6, 2015, at 12:54 PM, Roberta Horner wrote: > > The oddest things I cut were the honey bee and yellow jacket stingers. I've done plant stamen, reptiles, fish and I believe another insect. I usually tell the students that are working on a research project to give me a sample they don't care about so I can see if I can do what they want. > > But I had oddities that I didn't have to section like during hunting season a hunter killed a deer and there was a mass on the trachea that he wanted tested to make sure the deer was okay to eat. I got the sample and when I tried to gross it I found a very hard shiny silver object. I told the pathologist whose case it was that the mass was from a bullet did he still want histo done. No. > > The other interesting one was the egg shell. > The conversation went something like this. > Pathologist: Can you section this egg shell > Me: No it's too hard. > P: Can't you decal it > M: That's not going to work. > P: Did you try. > M: No > P: Don't you think you should try first. > M: Okay fine but it is no going to work. > > Put a piece of eggshell (made of calcium) into some decal solution (that removes calcium) and watch the egg shell bubble and disappear. I did get to tell the pathologist "I told you so" > > Roberta Horner > Penn State University > Animal Diagnostic Lab > > -----Original Message----- > From: Morken, Timothy [mailto:Timothy.Morken@ucsf.edu] > Sent: Tuesday, January 06, 2015 2:24 PM > To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > Subject: And other crazy stuff. RE: [Histonet] cutting honey bees > > You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? > > For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. > > > Open the floodgates.... > > Tim Morken > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg > Sent: Tuesday, January 06, 2015 11:13 AM > To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > Subject: RE: [Histonet] cutting honey bees > > for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. > > Cheers, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > pruegghm@hotmail.com > > > >> From: rjr6@psu.edu >> To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu >> Date: Sat, 3 Jan 2015 23:15:33 +0000 >> Subject: RE: [Histonet] cutting honey bees >> CC: >> >> I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. >> Roberta Horner >> Penn State University >> Animal Diagnostic Lab >> ________________________________________ >> From: histonet-bounces@lists.utsouthwestern.edu >> [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg >> [classicdoc@gmail.com] >> Sent: Saturday, January 03, 2015 6:08 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] cutting honey bees >> >> Has anyone had experience embedding and cutting honey bees. I am sure >> there are some issues with the harder exoskeleton. Would that have to >> be dissected away first. I am considering helping a student with a >> science fair project on bees. >> >> Douglas Gregg >> Veterianary pathologist >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> email.arizona.edu Tue Jan 6 22:23:09 2015 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue Jan 6 22:23:19 2015 Subject: [Histonet] cutting honey bees In-Reply-To: References: , , Message-ID: I processed honeybees and was successful sectioning them. It takes a bit of patience and time. I soaked the bees to soften the outer parts in glycerin water and or mollifex. As for processing, I had to make up a schedule and infiltration is the key. If it isn't important to see the whole bee you can section out the parts you need to see most and then it becomes easy. I'm slowly working on my notes and someday I'll get them all sorted out. Andi G ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Patsy Ruegg [pruegghm@hotmail.com] Sent: Tuesday, January 06, 2015 12:13 PM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com > From: rjr6@psu.edu > To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu > Date: Sat, 3 Jan 2015 23:15:33 +0000 > Subject: RE: [Histonet] cutting honey bees > CC: > > I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. > Roberta Horner > Penn State University > Animal Diagnostic Lab > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classicdoc@gmail.com] > Sent: Saturday, January 03, 2015 6:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cutting honey bees > > Has anyone had experience embedding and cutting honey bees. I am sure > there are some issues with the harder exoskeleton. Would that have to > be dissected away first. I am considering helping a student with a > science fair project on bees. > > Douglas Gregg > Veterianary pathologist > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Wed Jan 7 08:14:26 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Wed Jan 7 08:14:40 2015 Subject: And other crazy stuff. RE: [Histonet] cutting honey bees In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> Message-ID: You guys are so cool!! Thanks for sharing your stories - I love reading all of the cool stuff histotechs are doing out in the world! Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 1/6/15, 5:38 PM, "Caroline Miller" wrote: >When I worked in a research core (which was only last week, but I just >changed jobs). I have processed and cut (with varying degrees of success) >fake meat samples for a company. They were mainly made of grains and >mushed veggies. > >The problem was the samples were not consistent and there was no room >(time or money) for honing the protocol for each of the many samples he >sent me, so he got what I could cut. He always seemed pleased, but I >couldn't see much in the samples. He was very secretive about it all, so >I could never quite understand what they were doing t all for!!! > >C > >Sent from my iPhone > >> On Jan 6, 2015, at 12:54 PM, Roberta Horner wrote: >> >> The oddest things I cut were the honey bee and yellow jacket stingers. >>I've done plant stamen, reptiles, fish and I believe another insect. I >>usually tell the students that are working on a research project to give >>me a sample they don't care about so I can see if I can do what they >>want. >> >> But I had oddities that I didn't have to section like during hunting >>season a hunter killed a deer and there was a mass on the trachea that >>he wanted tested to make sure the deer was okay to eat. I got the >>sample and when I tried to gross it I found a very hard shiny silver >>object. I told the pathologist whose case it was that the mass was from >>a bullet did he still want histo done. No. >> >> The other interesting one was the egg shell. >> The conversation went something like this. >> Pathologist: Can you section this egg shell >> Me: No it's too hard. >> P: Can't you decal it >> M: That's not going to work. >> P: Did you try. >> M: No >> P: Don't you think you should try first. >> M: Okay fine but it is no going to work. >> >> Put a piece of eggshell (made of calcium) into some decal solution >>(that removes calcium) and watch the egg shell bubble and disappear. I >>did get to tell the pathologist "I told you so" >> >> Roberta Horner >> Penn State University >> Animal Diagnostic Lab >> >> -----Original Message----- >> From: Morken, Timothy [mailto:Timothy.Morken@ucsf.edu] >> Sent: Tuesday, January 06, 2015 2:24 PM >> To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu >> Subject: And other crazy stuff. RE: [Histonet] cutting honey bees >> >> You crazy research people...OK, so what is the craziest thing you ever >>had to cut, or were asked to cut? >> >> For me, not too bad, but embedding for EM and sectioning a single >>oocyte that was nearly microscopic. I'll just say it took a LOT of thick >>sections too face down to it without actually cutting through it. >> >> >> Open the floodgates.... >> >> Tim Morken >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy >>Ruegg >> Sent: Tuesday, January 06, 2015 11:13 AM >> To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu >> Subject: RE: [Histonet] cutting honey bees >> >> for the whole bee I probably would process and embed it in glycol >>methacrylate (gma) it is much harder and would give better sections, we >>have done zebra fish and several other harder tissues including >>calcified bone in GMA. >> >> Cheers, >> Patsy >> >> Patsy Ruegg, HT(ASCP)QIHC >> Ruegg IHC Consulting >> 40864 E Arkansas Ave >> Bennett, CO 80102 >> H 303-644-4538 >> C 720-281-5406 >> pruegghm@hotmail.com >> >> >> >>> From: rjr6@psu.edu >>> To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu >>> Date: Sat, 3 Jan 2015 23:15:33 +0000 >>> Subject: RE: [Histonet] cutting honey bees >>> CC: >>> >>> I sectioned and stained honey bee and yellow jacket stingers years >>>ago. They wanted to show the difference between the stingers. I >>>wasn't sure what to do so I processed and handled like everything else. >>> I was able to get some good sections. I put 6 stingers in each block >>>and cut several sections figuring there should be at least one good >>>stinger in each block and it worked. >>> Roberta Horner >>> Penn State University >>> Animal Diagnostic Lab >>> ________________________________________ >>> From: histonet-bounces@lists.utsouthwestern.edu >>> [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg >>> [classicdoc@gmail.com] >>> Sent: Saturday, January 03, 2015 6:08 PM >>> To: histonet@lists.utsouthwestern.edu >>> Subject: [Histonet] cutting honey bees >>> >>> Has anyone had experience embedding and cutting honey bees. I am sure >>> there are some issues with the harder exoskeleton. Would that have to >>> be dissected away first. I am considering helping a student with a >>> science fair project on bees. >>> >>> Douglas Gregg >>> Veterianary pathologist >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ryan.Roy <@t> va.gov Wed Jan 7 09:31:06 2015 From: Ryan.Roy <@t> va.gov (Roy, Ryan) Date: Wed Jan 7 09:32:09 2015 Subject: [EXTERNAL] RE: [Histonet] cutting honey bees In-Reply-To: References: , Message-ID: <15F883394EAB744E99E1C7E1B98730490177061C2EB7@R04BYNMSGB1.r04.med.va.gov> I agree with Patsy. If you have a access to a "plastic histo lab", that would be the best, as you could easily section any part of the Bee's anatomy. Ryan Roy HTL (ASCP) Manchester Veterans Affairs Medical Center Manchester New Hampshire Disclosure: The content of this email does not represent the views or opinons of the VA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 2:13 PM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: [EXTERNAL] RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com > From: rjr6@psu.edu > To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu > Date: Sat, 3 Jan 2015 23:15:33 +0000 > Subject: RE: [Histonet] cutting honey bees > CC: > > I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. > Roberta Horner > Penn State University > Animal Diagnostic Lab > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg > [classicdoc@gmail.com] > Sent: Saturday, January 03, 2015 6:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cutting honey bees > > Has anyone had experience embedding and cutting honey bees. I am sure > there are some issues with the harder exoskeleton. Would that have to > be dissected away first. I am considering helping a student with a > science fair project on bees. > > Douglas Gregg > Veterianary pathologist > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jvickroy <@t> SpringfieldClinic.com Wed Jan 7 12:24:42 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Wed Jan 7 12:24:50 2015 Subject: [Histonet] ASP300S or VIP 6 Message-ID: <9B1A1501A800064397369BD8072E6BCA96A923@E2K10DB.springfieldclinic.com> Buying two automated tissue processors for new lab. I have always used the VIP tissue processors, can anyone comment on a side by side comparison between the Leica and the VIP 6? thanks Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From lblazek <@t> digestivespecialists.com Wed Jan 7 12:50:00 2015 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Wed Jan 7 12:50:17 2015 Subject: [Histonet] control Message-ID: <5A2BD13465E061429D6455C8D6B40E3917313675A8@IBMB7Exchange.digestivespecialists.com> Hello all, I was wondering if there is anyone that may have a positive HSV control that they would be willing to share. I would greatly appreciate it! Thanks in advance, Linda Linda Blazek HT (ASCP) GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com From Lacie.Algeo <@t> providence.org Wed Jan 7 14:34:20 2015 From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A) Date: Wed Jan 7 14:34:35 2015 Subject: [Histonet] slide distribution Message-ID: <24C4B3C167E5694887AB594C7602CE3A2306DC@WN35104.or.providence.org> Hi All, I know there are different schools of thought on slide distribution methods. I am trying to move from pre-assigning cases to a continuous flow delivery to read system. Does anyone have any protocols, feedback, data, literature etc. on this that would be helpful? We are currently wasting a lot of time and money on pre-assigning (including when changes need to be made etc.). I am also finding that TAT suffers due to Paths waiting on assigned cases to be completed when other Paths have a stack of cases waiting to be read because theirs just happened to come out first. Thank you!!! Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From rjbuesa <@t> yahoo.com Wed Jan 7 15:14:33 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jan 7 15:14:39 2015 Subject: [Histonet] slide distribution In-Reply-To: <24C4B3C167E5694887AB594C7602CE3A2306DC@WN35104.or.providence.org> References: <24C4B3C167E5694887AB594C7602CE3A2306DC@WN35104.or.providence.org> Message-ID: <380709951.3605803.1420665273526.JavaMail.yahoo@jws10097.mail.ne1.yahoo.com> You have just identified your?"bottle neck": it is the pathologists. You have to go by what they want to do and how they receive the cases.In my case? my concern were the "Rush" cases that I had always ready (ALL) at 8AM and were brought to the path. office and left on an assigned spot.From there the pathologists took what they wanted.Similarly the rest of the cases were brought to the office in batches until all were finished (never after noon every day).In another lab I managed it was instituted the "pre-sorting" and it was just a chaos with, as you describe, cases?piling up in so pathologists?compromising the TAT in a way that histology was finished on time, but the office, as a whole, had sometimes 11 days delays.Talk to the office manager (if you have one), and with the chief pathologists that the best way is to leave the finished cases at the office and from there the pathologists would pick their cases.Ren? J. On Wednesday, January 7, 2015 3:34 PM, "Algeo, Lacie A" wrote: Hi All, I know there are different schools of thought on slide distribution methods.? I am trying to move from pre-assigning cases to a continuous flow delivery to read system.? Does anyone have any protocols, feedback, data, literature etc. on this that would be helpful?? We are currently wasting a lot of time and money on pre-assigning (including when changes need to be made etc.).? I am also finding that TAT suffers due to Paths waiting on assigned cases to be completed when other Paths have a stack of cases waiting to be read because theirs just happened to come out first. Thank you!!! Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law.? If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message.? If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patrick.lewis <@t> seattlechildrens.org Wed Jan 7 17:42:11 2015 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Wed Jan 7 17:42:19 2015 Subject: [Histonet] Cryostat troublshooting. CM3050 Message-ID: <3903BE18914F4440834F0E620415FFCA3CB52233@PPWEXD01d.childrens.sea.kids> H everyone, We have been using this old CM3050 cryostat for our Frozen OCT blocks. I am cutting at 5 uM. My issue is that when I use the hand rotator to cut my sections it doesn't seem to advance with each rotation. It can take as many as 4 rotations to go from one section to another. What would cause this, and is there an easy solution to this problem? I can use the button to automatically move the chick holder all the forward and back from blinking to blinking, so I am wondering if ice crystals or debris can be ruled out as the issue. Any advice welcome. Thanks Patrick. ps: when I do get the blade to cut into the block, I have to wonder if the section I am getting is really 5 uM, or is it thicker. Sometimes you can tell if the section looks particularly thick, but other times it's hard to tell. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From hans <@t> histologistics.com Wed Jan 7 18:18:54 2015 From: hans <@t> histologistics.com (Hans B Snyder) Date: Wed Jan 7 18:18:59 2015 Subject: [Histonet] Cryostat troublshooting. CM3050 In-Reply-To: <3903BE18914F4440834F0E620415FFCA3CB52233@PPWEXD01d.childrens.sea.kids> References: <3903BE18914F4440834F0E620415FFCA3CB52233@PPWEXD01d.childrens.sea.kids> Message-ID: Hello Patrick, We have this same cryostat except it's the CM 3050S. Ours also has this issue usually after it's been running for many months with a defrost. The typical cause of your problem is the cylinder on which the block holder is attached has ice crystals or is "frozen". I feel the cause of this is because the glass was left open too long while cutting. This allowed moister from the room to condense on the cylinder and subsequently causes a stop go motion. Since I am in charge of all histology equipment maintenance, my solution is to turn off the cryostat, take the microtome out of the cavity and let it dry overnight. Then in the morning, I physically dry the microtome. Using cryostat oil, oil all the moving parts including the cylinder, then wipe off excess. Before putting the microtome back into the cavity, the cavity itself must also be thoroughly dried. The final cool down process usually takes about 1-2 hours. Feel free to email any specific questions. Hope this helps. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Wed, Jan 7, 2015 at 6:42 PM, Lewis, Patrick wrote: > H everyone, > > We have been using this old CM3050 cryostat for our Frozen OCT blocks. > > I am cutting at 5 uM. > > My issue is that when I use the hand rotator to cut my sections it doesn't seem to advance with each rotation. It can take as many as 4 rotations to go from one section to another. > > What would cause this, and is there an easy solution to this problem? > > I can use the button to automatically move the chick holder all the forward and back from blinking to blinking, so I am wondering if ice crystals or debris can be ruled out as the issue. > > Any advice welcome. > > Thanks > > Patrick. > > ps: when I do get the blade to cut into the block, I have to wonder if the section I am getting is really 5 uM, or is it thicker. Sometimes you can tell if the section looks particularly thick, but other times it's hard to tell. > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.bernard <@t> comcast.net Wed Jan 7 19:04:53 2015 From: ian.bernard <@t> comcast.net (ian bernard) Date: Wed Jan 7 19:05:14 2015 Subject: [Histonet] ASP300S or VIP 6 In-Reply-To: <9B1A1501A800064397369BD8072E6BCA96A923@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA96A923@E2K10DB.springfieldclinic.com> Message-ID: <00ef01d02adf$1cfc3a30$56f4ae90$@comcast.net> James, we have both the VIP-5 and Leica ASP300S. While the VIP has sustained us well through the years, notwithstanding, with malfunctions since it's on its last leg; the ASP 300S was our researched replacement and/or alternate processor of choice. Since our validations and use of both processors in parallel , we are happy to have made the transition to the ASP-300S. It is user friendly, with nice graphics, and with the operating manual on the screen and a niche reagent management system as well as the auto fill feature are excellent options to have. IRB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Wednesday, January 07, 2015 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASP300S or VIP 6 Buying two automated tissue processors for new lab. I have always used the VIP tissue processors, can anyone comment on a side by side comparison between the Leica and the VIP 6? thanks Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.bernard <@t> comcast.net Wed Jan 7 19:08:24 2015 From: ian.bernard <@t> comcast.net (ian bernard) Date: Wed Jan 7 19:08:37 2015 Subject: [Histonet] Automatic H & E slide stainer recommendations In-Reply-To: References: Message-ID: <00f601d02adf$9aaf7cd0$d00e7670$@comcast.net> The Leica Autostainer XL has proven effective for our lab. IRB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, January 06, 2015 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H & E slide stainer recommendations Hello colleagues, I would appreciate any recommendations for an automatic slide stainer. It will primarily be used for H & E staining, not IHC. Thanks in advance, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.bernard <@t> comcast.net Thu Jan 8 06:22:46 2015 From: ian.bernard <@t> comcast.net (ian bernard) Date: Thu Jan 8 06:23:11 2015 Subject: [Histonet] ASP300S or VIP 6 In-Reply-To: <004c01d02aec$03cf7380$0b6e5a80$@gbi-inc.com> References: <9B1A1501A800064397369BD8072E6BCA96A923@E2K10DB.springfieldclinic.com> <00ef01d02adf$1cfc3a30$56f4ae90$@comcast.net> <004c01d02aec$03cf7380$0b6e5a80$@gbi-inc.com> Message-ID: <001901d02b3d$cf8f2a50$6ead7ef0$@comcast.net> Rachel, thanks for the question. For us, our reagent cost is comparable other than now we have another processor so will have to acquire more reagents to support both since we plan on running them both daily. However, another feature with the ASP 300 is that owing to the Reagent Management System our reagents can last 3 times longer than reagent we used on the VIP-5, per our protocol. It also has a paraffin cleaning system. Where it removes residue clearing agent from the paraffin thus preserving the life of the paraffin as well. And, in case all are wondering, I am not a spoke person for Leica or the ASP300S. Just a happy and practical customer with his new equipment. IRB -----Original Message----- From: Rachel Gonzalez [mailto:rachel@gbi-inc.com] Sent: Wednesday, January 07, 2015 7:37 PM To: 'ian bernard' Subject: RE: [Histonet] ASP300S or VIP 6 Hi Are the reagent cost comparable. Thanks Rachel -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of ian bernard Sent: Wednesday, January 7, 2015 5:05 PM To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASP300S or VIP 6 James, we have both the VIP-5 and Leica ASP300S. While the VIP has sustained us well through the years, notwithstanding, with malfunctions since it's on its last leg; the ASP 300S was our researched replacement and/or alternate processor of choice. Since our validations and use of both processors in parallel , we are happy to have made the transition to the ASP-300S. It is user friendly, with nice graphics, and with the operating manual on the screen and a niche reagent management system as well as the auto fill feature are excellent options to have. IRB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Wednesday, January 07, 2015 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ASP300S or VIP 6 Buying two automated tissue processors for new lab. I have always used the VIP tissue processors, can anyone comment on a side by side comparison between the Leica and the VIP 6? thanks Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Toni.Rathborne <@t> rwjuh.edu Thu Jan 8 08:45:53 2015 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Thu Jan 8 08:46:02 2015 Subject: [Histonet] Research cases Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F07170@vap1014.win.rwjuh.edu> Good morning everyone! I wanted to ask what others are doing about charges when blocks & slides are being requested for research purposes. We have been asked to provide a cost for this service, because the research companies are willing to pay the lab for our time processing, embedding, and cutting slides specifically for this purpose. Any guidance in this would be greatly appreciated! Toni Rathborne Pathology Supervisor From jvickroy <@t> SpringfieldClinic.com Thu Jan 8 08:52:27 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Thu Jan 8 08:52:49 2015 Subject: [Histonet] Slide and cassette labelers Message-ID: <9B1A1501A800064397369BD8072E6BCA96AA60@E2K10DB.springfieldclinic.com> Has anyone used the Tissue Tex "SmartWrite" Slide Printer and Cassette Printer? These both use Thermal transfer. I am also considering ThermoScientific, Leica, and General Data. Any advice would be welcome. Thanks Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From rjbuesa <@t> yahoo.com Thu Jan 8 08:55:04 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 8 08:55:11 2015 Subject: [Histonet] Research cases In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24F07170@vap1014.win.rwjuh.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24F07170@vap1014.win.rwjuh.edu> Message-ID: <21638582.3839996.1420728904579.JavaMail.yahoo@jws10046.mail.ne1.yahoo.com> You should know your own costs for the whole process and you should add a "fair" amount as an external service.Talk with your lab manager and get to a consensus on how to proceed but your costs have to be your essential guide.Ren? J.? On Thursday, January 8, 2015 9:45 AM, "Rathborne, Toni" wrote: Good morning everyone! I wanted to ask what others are doing about charges when blocks & slides are being requested for research purposes. We have been asked to provide a cost for this service, because the research companies are willing to pay the lab for our time processing, embedding, and cutting slides specifically for this purpose. Any guidance in this would be greatly appreciated! Toni Rathborne Pathology Supervisor _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TGoins <@t> mt.gov Thu Jan 8 09:13:28 2015 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Jan 8 09:15:48 2015 Subject: [Histonet] RE: Research cases In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24F07170@vap1014.win.rwjuh.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24F07170@vap1014.win.rwjuh.edu> Message-ID: We charge by the hour. The condition of the samples when received varies greatly (container, sample ID, master list, etc.) and by the hour rewards those who are organized and charges a fair rate for those who are not so organized. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, January 08, 2015 7:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Research cases Good morning everyone! I wanted to ask what others are doing about charges when blocks & slides are being requested for research purposes. We have been asked to provide a cost for this service, because the research companies are willing to pay the lab for our time processing, embedding, and cutting slides specifically for this purpose. Any guidance in this would be greatly appreciated! Toni Rathborne Pathology Supervisor From lblazek <@t> digestivespecialists.com Thu Jan 8 09:16:34 2015 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Jan 8 09:16:45 2015 Subject: [Histonet] RE: Slide and cassette labelers In-Reply-To: <9B1A1501A800064397369BD8072E6BCA96AA60@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA96AA60@E2K10DB.springfieldclinic.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E3917313677A8@IBMB7Exchange.digestivespecialists.com> You might also look at the Primera printers sold by Creative Waste Solutions. I have both the slide and cassette printer and like them both. They are nice and compact. http://cwsincorp.com/ Linda Linda Blazek HT (ASCP) Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Thursday, January 08, 2015 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide and cassette labelers Has anyone used the Tissue Tex "SmartWrite" Slide Printer and Cassette Printer? These both use Thermal transfer. I am also considering ThermoScientific, Leica, and General Data. Any advice would be welcome. Thanks Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Thu Jan 8 09:17:07 2015 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Thu Jan 8 09:17:29 2015 Subject: [Histonet] New 88344 CPT code question Message-ID: <9BF2DFCF-7AE3-4F9C-857D-EE77FA7F47F4@imagesbyhopper.com> Hello Histonetters :) With regards to billing for multiplex IHC stains, I have a scenario and question. Scenario: 1 container, 2 blocks, 2 different cocktail IHC stains. Stain1 is done on both blocks A1 and A2. Stain2 is done on block A1. How would we bill for this? Would it be 88344X2 - because there are two distinct cocktails. OR would it be 88344X1 - because you can only charge one per container? Thanks in advance, Michelle Sent from my iPhone From melissa <@t> alliedsearchpartners.com Thu Jan 8 10:01:12 2015 From: melissa <@t> alliedsearchpartners.com (Melissa Owens) Date: Thu Jan 8 10:01:21 2015 Subject: [Histonet] Histology Supervisor Job Opening in Buffalo, NY Message-ID: Hello and Happy New Year. My firm has a histology supervisor opening in Buffalo, NY. It requires at least a year of histology supervisor or lead or senior experience within a clinical histology department and a new york clinical lab or histology license. Please contact me if you are interested and I can give you more details. Thank you, Melissa Owens (Phelan) President, Laboratory Staffing Allied Search Partners From hans <@t> histologistics.com Thu Jan 8 10:12:00 2015 From: hans <@t> histologistics.com (Hans B Snyder) Date: Thu Jan 8 10:12:20 2015 Subject: [Histonet] Celestin Blue B In-Reply-To: References: Message-ID: Hello, Just in case you didn't find this yet, Rowley Biochemical in Danvers MA sells this. http://www.rowleybio.com/order.php Good luck! Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Tue, Jan 6, 2015 at 5:18 PM, John Smallwood wrote: > Hello Histologists: > Is there anywhere to get this ? Celestin Blue B C.I. 51050 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kristopher.Kalleberg <@t> unilever.com Thu Jan 8 11:53:38 2015 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Thu Jan 8 11:54:08 2015 Subject: [Histonet] X-gal staining Message-ID: Hello All, Does anyone know of a commercially available X-gal stain that would work on formalin fixed paraffin embedded skin samples in order to detect beta galactosidase. Thank you in advance. Kris From koellingr <@t> comcast.net Thu Jan 8 12:28:04 2015 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Thu Jan 8 12:28:25 2015 Subject: [Histonet] X-gal staining In-Reply-To: References: Message-ID: <1040313959.16559570.1420741684071.JavaMail.zimbra@comcast.net> Kristopher, beta-gal is an enzyme and as far as I know is rendered inert after FFPE.? I did do fresh skin in X-gal to target, as you would with any whole mount specimen, THEN FFPE and cut sections to it to see the signal.? If that is not an option, go after it with an anti-beta galactosidase antibody (polyclonal-like Pierce I used-have zero relationship to them) from several places.? Make sure the immunogen is a full, length protein that produced the antibody, and it will go much easier. ? Ray ? Raymond Koelling Seattle, WA area ----- Original Message ----- From: "Kristopher Kalleberg" To: histonet-request@lists.utsouthwestern.edu, histonet@lists.utsouthwestern.edu Sent: Thursday, January 8, 2015 9:53:38 AM Subject: [Histonet] X-gal staining Hello All, Does anyone know of a commercially available X-gal stain that would work on formalin fixed paraffin embedded skin samples in order to detect beta galactosidase. ?Thank you in advance. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Thu Jan 8 12:43:41 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Thu Jan 8 12:44:16 2015 Subject: [Histonet] Anyone using Biogenex stainers Message-ID: <761E2B5697F795489C8710BCC72141FF367E8F97@ex07.net.ucsf.edu> Does anyone use a Biogenex stainer these days? They used to be a premier company but seem to have disappeared over the last decade. They still display at NSH and have ads in various places but am not aware of any lab that uses one. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. From manderson <@t> biocare.net Thu Jan 8 12:56:01 2015 From: manderson <@t> biocare.net (Murray Anderson) Date: Thu Jan 8 12:56:10 2015 Subject: [Histonet] New 88344 CPT code question Message-ID: Dear Michelle, For CMS: All tests are still billed "per specimen-per procedure" All "G" codes have been replaced with the following: 88342 - 1st initial antibody stain procedure 88341 - each additional antibody stain procedure 88344 - each Multiplex stain procedure Assuming that your block 1 and block 2 came from the same specimen... Your Scenario: Stain1 - Block A1 = 1 x 88344 Stain1 - Block A2 = cannot bill Stain 2 - Block A1 = 1 x 88344 Regards, Murray Anderson -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Thursday, January 08, 2015 7:17 AM To: Histonet Subject: [Histonet] New 88344 CPT code question Hello Histonetters :) With regards to billing for multiplex IHC stains, I have a scenario and question. Scenario: 1 container, 2 blocks, 2 different cocktail IHC stains. Stain1 is done on both blocks A1 and A2. Stain2 is done on block A1. How would we bill for this? Would it be 88344X2 - because there are two distinct cocktails. OR would it be 88344X1 - because you can only charge one per container? Thanks in advance, Michelle Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jvickroy <@t> SpringfieldClinic.com Thu Jan 8 13:17:31 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Thu Jan 8 13:17:37 2015 Subject: [Histonet] Usage of Thermo Cassette and Slde Printer Message-ID: <9B1A1501A800064397369BD8072E6BCA96AAFB@E2K10DB.springfieldclinic.com> Could anyone comment on the Thermo Printmate and Slide Mate? I am considering going to it for labeling of cassettes and slides. Thanks Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From pruegghm <@t> hotmail.com Thu Jan 8 13:55:11 2015 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Thu Jan 8 13:55:20 2015 Subject: [Histonet] Anyone using Biogenex stainers In-Reply-To: <761E2B5697F795489C8710BCC72141FF367E8F97@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367E8F97@ex07.net.ucsf.edu> Message-ID: I was curious about that too. Don't they have that large fancy machine they claim does ISH and everything? Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com > From: Timothy.Morken@ucsf.edu > To: histonet@lists.utsouthwestern.edu > Date: Thu, 8 Jan 2015 18:43:41 +0000 > Subject: [Histonet] Anyone using Biogenex stainers > > Does anyone use a Biogenex stainer these days? They used to be a premier company but seem to have disappeared over the last decade. They still display at NSH and have ads in various places but am not aware of any lab that uses one. > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > Box 1656 > 505 Parnassus Ave > San Francisco, CA 94143 > USA > > 415.514-6042 (office) > tim.morken@ucsfmedctr.org > > CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Tue Jan 6 13:23:49 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Thu Jan 8 13:59:49 2015 Subject: And other crazy stuff. RE: [Histonet] cutting honey bees Message-ID: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates.... Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com > From: rjr6@psu.edu > To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu > Date: Sat, 3 Jan 2015 23:15:33 +0000 > Subject: RE: [Histonet] cutting honey bees > CC: > > I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. > Roberta Horner > Penn State University > Animal Diagnostic Lab > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg > [classicdoc@gmail.com] > Sent: Saturday, January 03, 2015 6:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cutting honey bees > > Has anyone had experience embedding and cutting honey bees. I am sure > there are some issues with the harder exoskeleton. Would that have to > be dissected away first. I am considering helping a student with a > science fair project on bees. > > Douglas Gregg > Veterianary pathologist > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Tue Jan 6 13:32:59 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Thu Jan 8 13:59:53 2015 Subject: [Histonet] RE: PASD muscle stains In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF367E7346@ex07.net.ucsf.edu> Tiffany, we use 10% acetic acid per the AFIP procedure (1968 edition) Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tiffany Passaro Sent: Monday, January 05, 2015 3:23 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] PASD muscle stains Greetings, I am looking for fixatives that others are using in their labs for the PASD stain on fresh frozen muscle tissue. Currently we are fixing in 10% NBF. Thanks in advance for any info on this. Tiffany DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peter2015newpower <@t> 163.com Thu Jan 8 04:27:32 2015 From: peter2015newpower <@t> 163.com (peter) Date: Thu Jan 8 13:59:54 2015 Subject: [Histonet] zirconia ceramic knife 2015! Message-ID: <58d7a2e1.3701c.14ac915ba48.Coremail.peter2015newpower@163.com> IEhpICBtYW5hZ2VyLCAKCgogR29vZCBkYXkhCk5lZWQgemlyY29uaWEgY2VyYW1pYyBrbmlmZSwg d2UgYXJlIHByb2Zlc3Npb25hbCBDaGluZXNlIGZhY3RvcnkgLgogSWYgIGhhdmUgaW50ZXJlc3Qs IHBscyBjb250YWN0IG1lLgogMycnLTcnJyAga25pZmUgIC8gICBrbmlmZSBzZXQgICAgLyAgdmVn ZXRhYmxlIHBlZWxlciwgc2Npc3Nzb3IgICAvICAgZm9yaywgc3Bvb24gICAvICAgIG5vYmxlIGdp ZnQgc2V0CgoKd2VsY29tZWQgZm9yIGlucXVpcnkuCksuUgpQZXRlciBjaGFuCgoKCg==From Timothy.Morken <@t> ucsf.edu Thu Jan 8 09:40:55 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Thu Jan 8 13:59:55 2015 Subject: [Histonet] RE: Research cases In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24F07170@vap1014.win.rwjuh.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24F07170@vap1014.win.rwjuh.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF367E7E8B@ex07.net.ucsf.edu> We do a lot of research work , but generally in-house projects. We charge the technical-only fee. For in-house academic research we offer a steep discount. For commercial work we have a client price, depending on how much work we do for them. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, January 08, 2015 6:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Research cases Good morning everyone! I wanted to ask what others are doing about charges when blocks & slides are being requested for research purposes. We have been asked to provide a cost for this service, because the research companies are willing to pay the lab for our time processing, embedding, and cutting slides specifically for this purpose. Any guidance in this would be greatly appreciated! Toni Rathborne Pathology Supervisor From linda.prasad <@t> health.nsw.gov.au Tue Jan 6 16:57:37 2015 From: linda.prasad <@t> health.nsw.gov.au (Linda Prasad (SCHN)) Date: Thu Jan 8 14:02:54 2015 Subject: [Histonet] RE: PASD muscle stains In-Reply-To: References: Message-ID: <1217DDB3D7DE5E418E3D560A268EABD0A27DB0AD@xmdb03.nch.kids> Usually we do the DiPAS stain on muscle frozen section. Air dried for 10 minutes. Don't use any fixatives :) Linda Prasad, MSc, BSc Senior Scientist | Histopathology t: 02 9845 3306 | f: 02 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au m: 0425 314 267 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tiffany Passaro Sent: Tuesday, 6 January 2015 10:23 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] PASD muscle stains Greetings, I am looking for fixatives that others are using in their labs for the PASD stain on fresh frozen muscle tissue. Currently we are fixing in 10% NBF. Thanks in advance for any info on this. Tiffany DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* From bcooper <@t> chla.usc.edu Thu Jan 8 14:06:08 2015 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Thu Jan 8 14:06:17 2015 Subject: [Histonet] RE: PASD muscle stains In-Reply-To: <1217DDB3D7DE5E418E3D560A268EABD0A27DB0AD@xmdb03.nch.kids> References: <1217DDB3D7DE5E418E3D560A268EABD0A27DB0AD@xmdb03.nch.kids> Message-ID: Same here. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper@chla.usc.edu -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Prasad (SCHN) Sent: Tuesday, January 06, 2015 2:58 PM To: 'Tiffany Passaro'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: PASD muscle stains Usually we do the DiPAS stain on muscle frozen section. Air dried for 10 minutes. Don't use any fixatives :) Linda Prasad, MSc, BSc Senior Scientist | Histopathology t: 02 9845 3306 | f: 02 9845 3318 | e: linda.prasad@health.nsw.gov.au | w: www.schn.health.nsw.gov.au m: 0425 314 267 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tiffany Passaro Sent: Tuesday, 6 January 2015 10:23 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] PASD muscle stains Greetings, I am looking for fixatives that others are using in their labs for the PASD stain on fresh frozen muscle tissue. Currently we are fixing in 10% NBF. Thanks in advance for any info on this. Tiffany DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From mjones <@t> metropath.com Thu Jan 8 14:20:28 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Thu Jan 8 14:20:37 2015 Subject: And other crazy stuff. RE: [Histonet] cutting honey bees In-Reply-To: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> Message-ID: We did a goldfish once, interesting microscopically and difficult for peeling (lots of keratin?) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 1/6/15, 12:23 PM, "Morken, Timothy" wrote: >You crazy research people...OK, so what is the craziest thing you ever >had to cut, or were asked to cut? > >For me, not too bad, but embedding for EM and sectioning a single oocyte >that was nearly microscopic. I'll just say it took a LOT of thick >sections too face down to it without actually cutting through it. > > >Open the floodgates.... > >Tim Morken > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy >Ruegg >Sent: Tuesday, January 06, 2015 11:13 AM >To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu >Subject: RE: [Histonet] cutting honey bees > >for the whole bee I probably would process and embed it in glycol >methacrylate (gma) it is much harder and would give better sections, we >have done zebra fish and several other harder tissues including calcified >bone in GMA. > >Cheers, >Patsy > >Patsy Ruegg, HT(ASCP)QIHC >Ruegg IHC Consulting >40864 E Arkansas Ave >Bennett, CO 80102 >H 303-644-4538 >C 720-281-5406 >pruegghm@hotmail.com > > > >> From: rjr6@psu.edu >> To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu >> Date: Sat, 3 Jan 2015 23:15:33 +0000 >> Subject: RE: [Histonet] cutting honey bees >> CC: >> >> I sectioned and stained honey bee and yellow jacket stingers years ago. >> They wanted to show the difference between the stingers. I wasn't sure >>what to do so I processed and handled like everything else. I was able >>to get some good sections. I put 6 stingers in each block and cut >>several sections figuring there should be at least one good stinger in >>each block and it worked. >> Roberta Horner >> Penn State University >> Animal Diagnostic Lab >> ________________________________________ >> From: histonet-bounces@lists.utsouthwestern.edu >> [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg >> [classicdoc@gmail.com] >> Sent: Saturday, January 03, 2015 6:08 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] cutting honey bees >> >> Has anyone had experience embedding and cutting honey bees. I am sure >> there are some issues with the harder exoskeleton. Would that have to >> be dissected away first. I am considering helping a student with a >> science fair project on bees. >> >> Douglas Gregg >> Veterianary pathologist >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dhewitt <@t> hvhs.org Thu Jan 8 14:27:58 2015 From: dhewitt <@t> hvhs.org (Daniel Hewitt) Date: Thu Jan 8 14:28:05 2015 Subject: And other crazy stuff. RE: [Histonet] cutting honey bees In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> Message-ID: <7DDB5AB36CBC574D8D680806E7BBE58B02B16A94@MX-HVB-02.hvhs.org> I have done a stink bug, spider and a few other creepy crawlers for my kids to look at under the scope, they have no idea what they are looking at but still love it. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Ann Jones Sent: Thursday, January 08, 2015 3:20 PM To: Morken, Timothy; Patsy Ruegg; Roberta Horner; Douglas Gregg; 'histonet@lists.utsouthwestern.edu' Subject: Re: And other crazy stuff. RE: [Histonet] cutting honey bees We did a goldfish once, interesting microscopically and difficult for peeling (lots of keratin?) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 1/6/15, 12:23 PM, "Morken, Timothy" wrote: >You crazy research people...OK, so what is the craziest thing you ever >had to cut, or were asked to cut? > >For me, not too bad, but embedding for EM and sectioning a single oocyte >that was nearly microscopic. I'll just say it took a LOT of thick >sections too face down to it without actually cutting through it. > > >Open the floodgates.... > >Tim Morken > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy >Ruegg >Sent: Tuesday, January 06, 2015 11:13 AM >To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu >Subject: RE: [Histonet] cutting honey bees > >for the whole bee I probably would process and embed it in glycol >methacrylate (gma) it is much harder and would give better sections, we >have done zebra fish and several other harder tissues including calcified >bone in GMA. > >Cheers, >Patsy > >Patsy Ruegg, HT(ASCP)QIHC >Ruegg IHC Consulting >40864 E Arkansas Ave >Bennett, CO 80102 >H 303-644-4538 >C 720-281-5406 >pruegghm@hotmail.com > > > >> From: rjr6@psu.edu >> To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu >> Date: Sat, 3 Jan 2015 23:15:33 +0000 >> Subject: RE: [Histonet] cutting honey bees >> CC: >> >> I sectioned and stained honey bee and yellow jacket stingers years ago. >> They wanted to show the difference between the stingers. I wasn't sure >>what to do so I processed and handled like everything else. I was able >>to get some good sections. I put 6 stingers in each block and cut >>several sections figuring there should be at least one good stinger in >>each block and it worked. >> Roberta Horner >> Penn State University >> Animal Diagnostic Lab >> ________________________________________ >> From: histonet-bounces@lists.utsouthwestern.edu >> [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg >> [classicdoc@gmail.com] >> Sent: Saturday, January 03, 2015 6:08 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] cutting honey bees >> >> Has anyone had experience embedding and cutting honey bees. I am sure >> there are some issues with the harder exoskeleton. Would that have to >> be dissected away first. I am considering helping a student with a >> science fair project on bees. >> >> Douglas Gregg >> Veterianary pathologist >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegghm <@t> hotmail.com Thu Jan 8 14:41:55 2015 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Thu Jan 8 14:42:07 2015 Subject: And other crazy stuff. RE: [Histonet] cutting honey bees In-Reply-To: <7DDB5AB36CBC574D8D680806E7BBE58B02B16A94@MX-HVB-02.hvhs.org> References: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> , <7DDB5AB36CBC574D8D680806E7BBE58B02B16A94@MX-HVB-02.hvhs.org> Message-ID: we once played an april's fool joke on our Heme Pathologist. We took the wings off a fly and embedded it in plastic (GMA) sectioned and stained it. Grossly It looked similar to the bone marrow core biopsies we did. Heme pathologist are notorious for sticking slides under the microscope on high power without looking at them grossly. We said we needed him to consult on this really strange bone marrow core because we couldn't figure out what the patient had. Most of our patients at the time were patients with leukemia or lymphoma. Sure enough he stuck the slide under high power and probably even under oil immersion. Trying to look at the morphology of individual cells. He was stumped and was going to show it to another colleague, so we had to confess that it was a fly and this was a AF joke. We thought he would have thought we were clever and laugh about it but he did not think it was funny. We never did anything like that again. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com > Subject: RE: And other crazy stuff. RE: [Histonet] cutting honey bees > Date: Thu, 8 Jan 2015 15:27:58 -0500 > From: dhewitt@hvhs.org > To: mjones@metropath.com; Timothy.Morken@ucsf.edu; pruegghm@hotmail.com; rjr6@psu.edu; classicdoc@gmail.com; histonet@lists.utsouthwestern.edu > > I have done a stink bug, spider and a few other creepy crawlers for my > kids to look at under the scope, they have no idea what they are looking > at but still love it. > > Daniel Hewitt > Histology Supervisor, HVS > 412-749-7371 > > This email, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure or distribution > is prohibited. If you are not the intended recipient, or an agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and delete and destroy all copies of > the original message, including attachments. > > Please note that any views or opinions presented in this e-mail are > solely those of the author and do not necessarily represent those of > Heritage Valley Health System. The integrity and security of this > message cannot be guaranteed on the internet. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael > Ann Jones > Sent: Thursday, January 08, 2015 3:20 PM > To: Morken, Timothy; Patsy Ruegg; Roberta Horner; Douglas Gregg; > 'histonet@lists.utsouthwestern.edu' > Subject: Re: And other crazy stuff. RE: [Histonet] cutting honey bees > > We did a goldfish once, interesting microscopically and difficult for > peeling (lots of keratin?) > Michael Ann Jones, HT (ASCP) > Histology Manager > Metropath > 7444 W. Alaska Dr. #250 > Lakewood, CO 80226 > 303.634.2511 > Mjones@metropath.com > > > > > On 1/6/15, 12:23 PM, "Morken, Timothy" wrote: > > >You crazy research people...OK, so what is the craziest thing you ever > >had to cut, or were asked to cut? > > > >For me, not too bad, but embedding for EM and sectioning a single > oocyte > >that was nearly microscopic. I'll just say it took a LOT of thick > >sections too face down to it without actually cutting through it. > > > > > >Open the floodgates.... > > > >Tim Morken > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy > >Ruegg > >Sent: Tuesday, January 06, 2015 11:13 AM > >To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > >Subject: RE: [Histonet] cutting honey bees > > > >for the whole bee I probably would process and embed it in glycol > >methacrylate (gma) it is much harder and would give better sections, we > >have done zebra fish and several other harder tissues including > calcified > >bone in GMA. > > > >Cheers, > >Patsy > > > >Patsy Ruegg, HT(ASCP)QIHC > >Ruegg IHC Consulting > >40864 E Arkansas Ave > >Bennett, CO 80102 > >H 303-644-4538 > >C 720-281-5406 > >pruegghm@hotmail.com > > > > > > > >> From: rjr6@psu.edu > >> To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu > >> Date: Sat, 3 Jan 2015 23:15:33 +0000 > >> Subject: RE: [Histonet] cutting honey bees > >> CC: > >> > >> I sectioned and stained honey bee and yellow jacket stingers years > ago. > >> They wanted to show the difference between the stingers. I wasn't > sure > >>what to do so I processed and handled like everything else. I was > able > >>to get some good sections. I put 6 stingers in each block and cut > >>several sections figuring there should be at least one good stinger in > >>each block and it worked. > >> Roberta Horner > >> Penn State University > >> Animal Diagnostic Lab > >> ________________________________________ > >> From: histonet-bounces@lists.utsouthwestern.edu > >> [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas > Gregg > >> [classicdoc@gmail.com] > >> Sent: Saturday, January 03, 2015 6:08 PM > >> To: histonet@lists.utsouthwestern.edu > >> Subject: [Histonet] cutting honey bees > >> > >> Has anyone had experience embedding and cutting honey bees. I am sure > >> there are some issues with the harder exoskeleton. Would that have to > >> be dissected away first. I am considering helping a student with a > >> science fair project on bees. > >> > >> Douglas Gregg > >> Veterianary pathologist > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Thu Jan 8 14:43:33 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Thu Jan 8 14:43:41 2015 Subject: And other crazy stuff. RE: [Histonet] cutting honey bees In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> Message-ID: You asked for it: squid tentacle tip, frog oocyte, serial sections through a entire zebra fish embryo, honey bee eyes, harbor seal artery, Andean mummy muscle (flaky little boogers), probably lots of others that I can't remember off the top of my head. Paula On Thu, Jan 8, 2015 at 3:20 PM, Michael Ann Jones wrote: > We did a goldfish once, interesting microscopically and difficult for > peeling (lots of keratin?) > Michael Ann Jones, HT (ASCP) > Histology Manager > Metropath > 7444 W. Alaska Dr. #250 > Lakewood, CO 80226 > 303.634.2511 > Mjones@metropath.com > > > > > On 1/6/15, 12:23 PM, "Morken, Timothy" wrote: > > >You crazy research people...OK, so what is the craziest thing you ever > >had to cut, or were asked to cut? > > > >For me, not too bad, but embedding for EM and sectioning a single oocyte > >that was nearly microscopic. I'll just say it took a LOT of thick > >sections too face down to it without actually cutting through it. > > > > > >Open the floodgates.... > > > >Tim Morken > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy > >Ruegg > >Sent: Tuesday, January 06, 2015 11:13 AM > >To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > >Subject: RE: [Histonet] cutting honey bees > > > >for the whole bee I probably would process and embed it in glycol > >methacrylate (gma) it is much harder and would give better sections, we > >have done zebra fish and several other harder tissues including calcified > >bone in GMA. > > > >Cheers, > >Patsy > > > >Patsy Ruegg, HT(ASCP)QIHC > >Ruegg IHC Consulting > >40864 E Arkansas Ave > >Bennett, CO 80102 > >H 303-644-4538 > >C 720-281-5406 > >pruegghm@hotmail.com > > > > > > > >> From: rjr6@psu.edu > >> To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu > >> Date: Sat, 3 Jan 2015 23:15:33 +0000 > >> Subject: RE: [Histonet] cutting honey bees > >> CC: > >> > >> I sectioned and stained honey bee and yellow jacket stingers years ago. > >> They wanted to show the difference between the stingers. I wasn't sure > >>what to do so I processed and handled like everything else. I was able > >>to get some good sections. I put 6 stingers in each block and cut > >>several sections figuring there should be at least one good stinger in > >>each block and it worked. > >> Roberta Horner > >> Penn State University > >> Animal Diagnostic Lab > >> ________________________________________ > >> From: histonet-bounces@lists.utsouthwestern.edu > >> [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg > >> [classicdoc@gmail.com] > >> Sent: Saturday, January 03, 2015 6:08 PM > >> To: histonet@lists.utsouthwestern.edu > >> Subject: [Histonet] cutting honey bees > >> > >> Has anyone had experience embedding and cutting honey bees. I am sure > >> there are some issues with the harder exoskeleton. Would that have to > >> be dissected away first. I am considering helping a student with a > >> science fair project on bees. > >> > >> Douglas Gregg > >> Veterianary pathologist > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rsrichmond <@t> gmail.com Thu Jan 8 14:44:26 2015 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Jan 8 14:44:31 2015 Subject: [Histonet] Decal acquired by StatLab Message-ID: I just got an e-mail noting that "StatLab Medical Products, the leading manufacturer, developer and distributor of cost-effective histology, cytology and immunohistochemistry consumable supplies, is pleased to announce the acquisition of Decal Chemical Corporation of Tallman, NY." Good time to be reminded that Decal? is a trademarked name, and is not the generic term for any bottle of decalcifier on the shelf. Decal was the most commonly used proprietary decalcifier when I started out in pathology fifty years ago. In a day when most labs prepared most of their own reagents, they always bought proprietary decalcifiers. Nowadays people buy all solutions. I think the Herrn Inschpektors get you if you home-brew. Bob Richmond Samurai Pathologist Maryville TN From pruegghm <@t> hotmail.com Thu Jan 8 14:47:13 2015 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Thu Jan 8 14:47:21 2015 Subject: [Histonet] Decal acquired by StatLab In-Reply-To: References: Message-ID: that is very interesting. I always used Decal Chemical products really liked their formic acid Immunocal reagent. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com > Date: Thu, 8 Jan 2015 15:44:26 -0500 > From: rsrichmond@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Decal acquired by StatLab > > I just got an e-mail noting that > > "StatLab Medical Products, the leading manufacturer, developer and > distributor of cost-effective histology, cytology and immunohistochemistry > consumable supplies, is pleased to announce the acquisition of Decal > Chemical Corporation of Tallman, NY." > > Good time to be reminded that Decal? is a trademarked name, and is not the > generic term for any bottle of decalcifier on the shelf. > > Decal was the most commonly used proprietary decalcifier when I started out > in pathology fifty years ago. In a day when most labs prepared most of > their own reagents, they always bought proprietary decalcifiers. > > Nowadays people buy all solutions. I think the Herrn Inschpektors get you > if you home-brew. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Thu Jan 8 14:50:47 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Jan 8 14:51:00 2015 Subject: And other crazy stuff. RE: [Histonet] cutting honey bees In-Reply-To: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> Message-ID: Termites for a science fair project. anchovy from a pizza to pull a joke on a pathologist chicken bone from dim sum chicken feet, again to pull one over on a pathologist. Not decalcified, embedded in resin. From: "Morken, Timothy" To: Patsy Ruegg , Roberta Horner , "Douglas Gregg" , "Histonet@Lists. Edu" Date: 01/08/2015 12:00 PM Subject: And other crazy stuff. RE: [Histonet] cutting honey bees Sent by: histonet-bounces@lists.utsouthwestern.edu You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates.... Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com > From: rjr6@psu.edu > To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu > Date: Sat, 3 Jan 2015 23:15:33 +0000 > Subject: RE: [Histonet] cutting honey bees > CC: > > I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. > Roberta Horner > Penn State University > Animal Diagnostic Lab > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg > [classicdoc@gmail.com] > Sent: Saturday, January 03, 2015 6:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cutting honey bees > > Has anyone had experience embedding and cutting honey bees. I am sure > there are some issues with the harder exoskeleton. Would that have to > be dissected away first. I am considering helping a student with a > science fair project on bees. > > Douglas Gregg > Veterianary pathologist > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hlukey <@t> msn.com Thu Jan 8 14:51:41 2015 From: hlukey <@t> msn.com (Hugh Luk) Date: Thu Jan 8 14:51:47 2015 Subject: [Histonet] RE: ASP300S or VIP 6 In-Reply-To: References: Message-ID: Hi Jim, Both machines have given us years of service and we (pretty) highly recommend either. However, part of your decision should encompass service. I recommend buying two of the same. Validation was easy (well, sort-of) on either machine. Notes and problems we've encountered with our machines: ASP300(S) "Thermal fuses" blow occasionally (if you do not premelt ALL the paraffin). Maybe 1-2/year. Also had a problem with a clog during the cleaning cycle (but resolved itself as was more a 'lazy tech' issue). Processing baskets are 3 x 100 and there are three paraffin chambers. Reagent containers "Low Level" are > 1 gallon (awe, have to get another bottle, to top off the container). VIP6: Our touchscreen malfunctioned one day after the warranty expired. Otherwise, the machine processes reagents similar to the ASP300 S and VIP5. Processing baskets are 2 x 150 and there are four, removable paraffin containers. Reagent containers are 1.0 gallons. I consider there problems small as our Biomed and back-up processors saved us. Best of luck making your choice. Hugh Hawaii > ------------------------------ > Date: Wed, 7 Jan 2015 18:24:42 +0000 > From: "Vickroy, James" > Subject: [Histonet] ASP300S or VIP 6 > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <9B1A1501A800064397369BD8072E6BCA96A923@E2K10DB.springfieldclinic.com> > Content-Type: text/plain; charset="us-ascii" > > > Buying two automated tissue processors for new lab. I have always used the VIP tissue processors, can anyone comment on a side by side comparison between the Leica and the VIP 6? > > thanks > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvickroy@SpringfieldClinic.com > > > This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. > ------------------------------ From JMacDonald <@t> mtsac.edu Thu Jan 8 15:08:06 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Jan 8 15:08:13 2015 Subject: [Histonet] plant processing Message-ID: I have a colleague at a neighboring school that would like to process plant tissue for materials for his botany class. Is there a good resource for plant processing? Is there anyone in southern California that is processing plant materials? Thank you, Jennifer From bcooper <@t> chla.usc.edu Thu Jan 8 17:33:42 2015 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Thu Jan 8 17:33:52 2015 Subject: [Histonet] Paraffin Temperature Checks Message-ID: For those of you out there that are fortunate enough to have tissue processors or embedding centers that are used less often than daily-like maybe even weekly (or even less frequently,) how often are you checking and documenting the paraffin temperatures on said pieces of equipment? For our daily equipment, it's no big deal obviously. But we have a "research" processor and embedding center that doesn't get used often-sometimes for a week or two, and if we're not touching the machine, it seems overkill to document paraffin temps daily. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper@chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From Timothy.Morken <@t> ucsf.edu Thu Jan 8 17:48:03 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Thu Jan 8 17:48:12 2015 Subject: [Histonet] RE: Paraffin Temperature Checks In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF367E9883@ex07.net.ucsf.edu> Brain, we write in NIU for Not in Use if it is not used on a particular day. However, you may want to do checks a couple times per week just to be sure it is working so you don't find solidified paraffin the day you want to use it! Since it takes just as much time to write NIU as it does to write down the temp, you might as well do it! Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Thursday, January 08, 2015 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Temperature Checks For those of you out there that are fortunate enough to have tissue processors or embedding centers that are used less often than daily-like maybe even weekly (or even less frequently,) how often are you checking and documenting the paraffin temperatures on said pieces of equipment? For our daily equipment, it's no big deal obviously. But we have a "research" processor and embedding center that doesn't get used often-sometimes for a week or two, and if we're not touching the machine, it seems overkill to document paraffin temps daily. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper@chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Jan 8 18:10:58 2015 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Jan 8 18:11:04 2015 Subject: [Histonet] RE: Paraffin Temperature Checks In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDF1D@SBS2K8.premierlab.local> All of our logs are use logs so we record when we use any piece of equipment and any QC or maintenance if required, we do not record or document anything if we do not use it, other than lab temperature and humidity which we record daily. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Thursday, January 08, 2015 4:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin Temperature Checks For those of you out there that are fortunate enough to have tissue processors or embedding centers that are used less often than daily-like maybe even weekly (or even less frequently,) how often are you checking and documenting the paraffin temperatures on said pieces of equipment? For our daily equipment, it's no big deal obviously. But we have a "research" processor and embedding center that doesn't get used often-sometimes for a week or two, and if we're not touching the machine, it seems overkill to document paraffin temps daily. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper@chla.usc.edu --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b427297 <@t> aol.com Thu Jan 8 21:23:24 2015 From: b427297 <@t> aol.com (b427297@aol.com) Date: Thu Jan 8 21:23:34 2015 Subject: [Histonet] RE: Paraffin Temperature Checks In-Reply-To: <14E2C6176416974295479C64A11CB9AE019C79ECDF1D@SBS2K8.premierlab.local> References: <14E2C6176416974295479C64A11CB9AE019C79ECDF1D@SBS2K8.premierlab.local> Message-ID: <1D1F782E-DDBD-4CDA-B078-7D8E56468DA9@aol.com> I like to use a chart recorder on paraffin dispensers in case the thing goes whacko over a weekend and boils the paraffin, then goes back to normal before anyone notices. Sent from my iPhone > On Jan 8, 2015, at 6:10 PM, Elizabeth Chlipala wrote: > > All of our logs are use logs so we record when we use any piece of equipment and any QC or maintenance if required, we do not record or document anything if we do not use it, other than lab temperature and humidity which we record daily. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > liz@premierlab.com > www.premierlab.com > > March 10, 2014 is Histotechnology Professionals Day > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian > Sent: Thursday, January 08, 2015 4:34 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Paraffin Temperature Checks > > For those of you out there that are fortunate enough to have tissue processors or embedding centers that are used less often than daily-like maybe even weekly (or even less frequently,) how often are you checking and documenting the paraffin temperatures on said pieces of equipment? For our daily equipment, it's no big deal obviously. But we have a "research" processor and embedding center that doesn't get used often-sometimes for a week or two, and if we're not touching the machine, it seems overkill to document paraffin temps daily. > > Thanks, > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 Pager: 213-209-0184 > bcooper@chla.usc.edu > > > > --------------------------------------------------------------------- > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > > --------------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Fri Jan 9 05:37:12 2015 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Jan 9 05:37:32 2015 Subject: [Histonet] Automatic H & E slide stainer recommendations In-Reply-To: <00f601d02adf$9aaf7cd0$d00e7670$@comcast.net> References: <00f601d02adf$9aaf7cd0$d00e7670$@comcast.net> Message-ID: <42CE3C79-0F27-4B0D-9495-91BB153DA129@imagesbyhopper.com> I second the Leica XL. That stainer is a reliable workhorse. Sent from my iPhone On Jan 7, 2015, at 8:08 PM, ian bernard wrote: The Leica Autostainer XL has proven effective for our lab. IRB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, January 06, 2015 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H & E slide stainer recommendations Hello colleagues, I would appreciate any recommendations for an automatic slide stainer. It will primarily be used for H & E staining, not IHC. Thanks in advance, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> imagesbyhopper.com Fri Jan 9 06:45:30 2015 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Fri Jan 9 06:45:40 2015 Subject: [Histonet] Tesr Message-ID: <64012373-BFBB-4E82-B18F-11D47363EC49@imagesbyhopper.com> Please disregard Sent from my iPhone From algranth <@t> email.arizona.edu Fri Jan 9 07:44:39 2015 From: algranth <@t> email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Fri Jan 9 07:44:46 2015 Subject: And other crazy stuff. RE: [Histonet] cutting honey bees In-Reply-To: References: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> , Message-ID: Shrew mandible, spittle bugs, white fly salivary glands, mosquito ovaries. ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Paula Sicurello [patpxs@gmail.com] Sent: Thursday, January 08, 2015 1:43 PM To: Michael Ann Jones Cc: histonet@lists.utsouthwestern.edu; Morken, Timothy; Roberta Horner; Douglas Gregg Subject: Re: And other crazy stuff. RE: [Histonet] cutting honey bees You asked for it: squid tentacle tip, frog oocyte, serial sections through a entire zebra fish embryo, honey bee eyes, harbor seal artery, Andean mummy muscle (flaky little boogers), probably lots of others that I can't remember off the top of my head. Paula On Thu, Jan 8, 2015 at 3:20 PM, Michael Ann Jones wrote: > We did a goldfish once, interesting microscopically and difficult for > peeling (lots of keratin?) > Michael Ann Jones, HT (ASCP) > Histology Manager > Metropath > 7444 W. Alaska Dr. #250 > Lakewood, CO 80226 > 303.634.2511 > Mjones@metropath.com > > > > > On 1/6/15, 12:23 PM, "Morken, Timothy" wrote: > > >You crazy research people...OK, so what is the craziest thing you ever > >had to cut, or were asked to cut? > > > >For me, not too bad, but embedding for EM and sectioning a single oocyte > >that was nearly microscopic. I'll just say it took a LOT of thick > >sections too face down to it without actually cutting through it. > > > > > >Open the floodgates.... > > > >Tim Morken > > > >-----Original Message----- > >From: histonet-bounces@lists.utsouthwestern.edu > >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy > >Ruegg > >Sent: Tuesday, January 06, 2015 11:13 AM > >To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > >Subject: RE: [Histonet] cutting honey bees > > > >for the whole bee I probably would process and embed it in glycol > >methacrylate (gma) it is much harder and would give better sections, we > >have done zebra fish and several other harder tissues including calcified > >bone in GMA. > > > >Cheers, > >Patsy > > > >Patsy Ruegg, HT(ASCP)QIHC > >Ruegg IHC Consulting > >40864 E Arkansas Ave > >Bennett, CO 80102 > >H 303-644-4538 > >C 720-281-5406 > >pruegghm@hotmail.com > > > > > > > >> From: rjr6@psu.edu > >> To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu > >> Date: Sat, 3 Jan 2015 23:15:33 +0000 > >> Subject: RE: [Histonet] cutting honey bees > >> CC: > >> > >> I sectioned and stained honey bee and yellow jacket stingers years ago. > >> They wanted to show the difference between the stingers. I wasn't sure > >>what to do so I processed and handled like everything else. I was able > >>to get some good sections. I put 6 stingers in each block and cut > >>several sections figuring there should be at least one good stinger in > >>each block and it worked. > >> Roberta Horner > >> Penn State University > >> Animal Diagnostic Lab > >> ________________________________________ > >> From: histonet-bounces@lists.utsouthwestern.edu > >> [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg > >> [classicdoc@gmail.com] > >> Sent: Saturday, January 03, 2015 6:08 PM > >> To: histonet@lists.utsouthwestern.edu > >> Subject: [Histonet] cutting honey bees > >> > >> Has anyone had experience embedding and cutting honey bees. I am sure > >> there are some issues with the harder exoskeleton. Would that have to > >> be dissected away first. I am considering helping a student with a > >> science fair project on bees. > >> > >> Douglas Gregg > >> Veterianary pathologist > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 <@t> gmail.com Fri Jan 9 08:07:05 2015 From: akemiat3377 <@t> gmail.com (Eileen Akemi Allison) Date: Fri Jan 9 08:07:11 2015 Subject: [Histonet] Levels for Gastic and Esophogeal Bx's Message-ID: Good morning Histoland: Happy Friday! I believe this question was asked last year, but I would like to revisit this again. I have worked in a variety of laboratories in my career, both small and large. Most of my experience was in Oregon with large hospital laboratories and University institutions. Back then, we cut (6) levels on all our bx?s, and picked-up (2) sections on each level. My current facility is cutting (3) levels and picking up (1) section on each level. I would like to ask what everyone else is doing. The reason I am asking is because our pathology director is requesting a change in our protocol to cut only (2) levels and (1) section at each level. Sincerely, Akemi Allison BS, HT/HTL (ASCP) From PAMarcum <@t> uams.edu Fri Jan 9 08:33:57 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Fri Jan 9 08:34:03 2015 Subject: [Histonet] Levels for Gastic and Esophogeal Bx's In-Reply-To: References: Message-ID: <512982655815410fb22caa2589f68381@MAIL13M2N2.ad.uams.edu> We do 3 levels with no more than 2 sections per level. It is what the pathologists prefer and therefore what we do. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eileen Akemi Allison Sent: Friday, January 09, 2015 8:07 AM To: Histonet Subject: [Histonet] Levels for Gastic and Esophogeal Bx's Good morning Histoland: Happy Friday! I believe this question was asked last year, but I would like to revisit this again. I have worked in a variety of laboratories in my career, both small and large. Most of my experience was in Oregon with large hospital laboratories and University institutions. Back then, we cut (6) levels on all our bx?s, and picked-up (2) sections on each level. My current facility is cutting (3) levels and picking up (1) section on each level. I would like to ask what everyone else is doing. The reason I am asking is because our pathology director is requesting a change in our protocol to cut only (2) levels and (1) section at each level. Sincerely, Akemi Allison BS, HT/HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Joyce.Weems <@t> emoryhealthcare.org Fri Jan 9 08:39:30 2015 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jan 9 08:39:45 2015 Subject: [Histonet] Levels for Gastic and Esophogeal Bx's In-Reply-To: <512982655815410fb22caa2589f68381@MAIL13M2N2.ad.uams.edu> References: <512982655815410fb22caa2589f68381@MAIL13M2N2.ad.uams.edu> Message-ID: Same here Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marcum, Pamela A Sent: Friday, January 09, 2015 9:34 AM To: 'Eileen Akemi Allison'; Histonet Subject: RE: [Histonet] Levels for Gastic and Esophogeal Bx's We do 3 levels with no more than 2 sections per level. It is what the pathologists prefer and therefore what we do. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eileen Akemi Allison Sent: Friday, January 09, 2015 8:07 AM To: Histonet Subject: [Histonet] Levels for Gastic and Esophogeal Bx's Good morning Histoland: Happy Friday! I believe this question was asked last year, but I would like to revisit this again. I have worked in a variety of laboratories in my career, both small and large. Most of my experience was in Oregon with large hospital laboratories and University institutions. Back then, we cut (6) levels on all our bx?s, and picked-up (2) sections on each level. My current facility is cutting (3) levels and picking up (1) section on each level. I would like to ask what everyone else is doing. The reason I am asking is because our pathology director is requesting a change in our protocol to cut only (2) levels and (1) section at each level. Sincerely, Akemi Allison BS, HT/HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From Timothy.Morken <@t> ucsf.edu Fri Jan 9 10:07:21 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Fri Jan 9 10:09:14 2015 Subject: [Histonet] Levels for Gastic and Esophogeal Bx's In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF367E99C8@ex07.net.ucsf.edu> Akemi, it is really up to the pathologists to decide what they want. In some cases there is literature promoting certain standards (e.g, Hirschprung's) but in most cases it is just the comfort level of the person reviewing the case. We have a large variety of levels and unstained protocols but for standard biopsies it is two levels on one slide, no unstained. We only cut unstained if they are ordered as part of a protocol or ordered specifically. We do not cut "extra" unstained "just in case." We had tens of thousands of unstained slides sitting in files "just in case" so I convinced them to stop that practice. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eileen Akemi Allison Sent: Friday, January 09, 2015 6:07 AM To: Histonet Subject: [Histonet] Levels for Gastic and Esophogeal Bx's Good morning Histoland: Happy Friday! I believe this question was asked last year, but I would like to revisit this again. I have worked in a variety of laboratories in my career, both small and large. Most of my experience was in Oregon with large hospital laboratories and University institutions. Back then, we cut (6) levels on all our bx?s, and picked-up (2) sections on each level. My current facility is cutting (3) levels and picking up (1) section on each level. I would like to ask what everyone else is doing. The reason I am asking is because our pathology director is requesting a change in our protocol to cut only (2) levels and (1) section at each level. Sincerely, Akemi Allison BS, HT/HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Joyce.Weems <@t> emoryhealthcare.org Fri Jan 9 10:20:52 2015 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jan 9 10:20:58 2015 Subject: [Histonet] Levels for Gastic and Esophogeal Bx's In-Reply-To: <761E2B5697F795489C8710BCC72141FF367E99C8@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367E99C8@ex07.net.ucsf.edu> Message-ID: And I should have added that we do two unstained at level 2 - if needed for alcian blue/PAS and H. pylori on all gastrics. We only do them when ordered. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph?s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Friday, January 09, 2015 11:07 AM To: 'Eileen Akemi Allison'; Histonet Subject: RE: [Histonet] Levels for Gastic and Esophogeal Bx's Akemi, it is really up to the pathologists to decide what they want. In some cases there is literature promoting certain standards (e.g, Hirschprung's) but in most cases it is just the comfort level of the person reviewing the case. We have a large variety of levels and unstained protocols but for standard biopsies it is two levels on one slide, no unstained. We only cut unstained if they are ordered as part of a protocol or ordered specifically. We do not cut "extra" unstained "just in case." We had tens of thousands of unstained slides sitting in files "just in case" so I convinced them to stop that practice. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eileen Akemi Allison Sent: Friday, January 09, 2015 6:07 AM To: Histonet Subject: [Histonet] Levels for Gastic and Esophogeal Bx's Good morning Histoland: Happy Friday! I believe this question was asked last year, but I would like to revisit this again. I have worked in a variety of laboratories in my career, both small and large. Most of my experience was in Oregon with large hospital laboratories and University institutions. Back then, we cut (6) levels on all our bx?s, and picked-up (2) sections on each level. My current facility is cutting (3) levels and picking up (1) section on each level. I would like to ask what everyone else is doing. The reason I am asking is because our pathology director is requesting a change in our protocol to cut only (2) levels and (1) section at each level. Sincerely, Akemi Allison BS, HT/HTL (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From robinsoc <@t> mercyhealth.com Fri Jan 9 11:25:35 2015 From: robinsoc <@t> mercyhealth.com (Cynthia Robinson) Date: Fri Jan 9 11:25:41 2015 Subject: [Histonet] Leica Aperio Message-ID: <4EE642D353925D4D96CB95E12427DBAE1F043944@NODCMSTMBX06.no.trinity-health.org> Does anyone have experience setting up a report template for a breast panel on the Aperio? We just got ours set up and haven't had training yet. The thing has been here since November but with holidays and schedules not working it has been a trial. Are trying to get a jumpstart before the pathologists start training and think it should be ready to go immediately. Thanks. Cindi Robinson HT(ASCP) Mercy Medical Center-Sioux City Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From jqb7 <@t> cdc.gov Fri Jan 9 11:46:54 2015 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Fri Jan 9 11:47:14 2015 Subject: [Histonet] Automatic H & E slide stainer recommendations In-Reply-To: <42CE3C79-0F27-4B0D-9495-91BB153DA129@imagesbyhopper.com> References: <00f601d02adf$9aaf7cd0$d00e7670$@comcast.net> <42CE3C79-0F27-4B0D-9495-91BB153DA129@imagesbyhopper.com> Message-ID: <3B2CD438E1628A41BD687E98B963B7812CA4A7DB@EMBX-CLFT4.cdc.gov> Agree -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histotech@imagesbyhopper.com Sent: Friday, January 09, 2015 6:37 AM To: ian bernard Cc: Subject: Re: [Histonet] Automatic H & E slide stainer recommendations I second the Leica XL. That stainer is a reliable workhorse. Sent from my iPhone On Jan 7, 2015, at 8:08 PM, ian bernard wrote: The Leica Autostainer XL has proven effective for our lab. IRB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, January 06, 2015 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H & E slide stainer recommendations Hello colleagues, I would appreciate any recommendations for an automatic slide stainer. It will primarily be used for H & E staining, not IHC. Thanks in advance, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wendy.roberts <@t> duke.edu Thu Jan 8 14:08:53 2015 From: wendy.roberts <@t> duke.edu (Wendy L Roberts) Date: Fri Jan 9 13:09:28 2015 Subject: [Histonet] Anyone using Biogenex stainers In-Reply-To: <20150108195929.2DD3A19AB67F_4AEE1A1B@mail-gw-01.oit.duke.edu> References: <761E2B5697F795489C8710BCC72141FF367E8F97@ex07.net.ucsf.edu>, <20150108195929.2DD3A19AB67F_4AEE1A1B@mail-gw-01.oit.duke.edu> Message-ID: <1420747734217.2263@dm.duke.edu> Hi Everyone, Our lab has just inherited an old Leica cryostat (2800 Frigocut-E), which uses the disc style specimen stages (no post). I've contacted Leica, but they no longer sell them, and the person I spoke to suggested that I contact this list for ideas. Thanks in advance for any and all help! Wendy Wendy L Roberts Research Analyst, Caron Lab Department of Cell Biology Duke University Medical Center Box 3287 DUMC Durham NC 27710 Wendy.Roberts@dm.duke.edu 919.681.4549 From kshevlin <@t> 7thwavelabs.com Thu Jan 8 16:25:40 2015 From: kshevlin <@t> 7thwavelabs.com (Kim Shevlin) Date: Fri Jan 9 13:09:33 2015 Subject: [Histonet] Slide and cassette labelers Message-ID: Hi Jim, I do not have experience with the Tissue Tex Smartwrite, but I have used the Leica and Thermoscientific printers. Of the two, I prefer the Leica brand cassette and slide printers. The barcodes and human readable are crystal clear. Hope this helps. Kim Kim Shevlin, HT ASCP Histology and Pathology Operations Manager Seventh Wave Laboratories, LLC 743 Spirit 40 Park Drive, Suite 108 Chesterfield, MO 63005 kshevlin@7thwavelabs.com www.7thwavelabs.com 636.519.4885 (Main Phone) 636.519.4776 (Histology Lab) 618.570.9383 (Cell Phone) From Behnaz.Sohrab <@t> ah.org Fri Jan 9 11:23:15 2015 From: Behnaz.Sohrab <@t> ah.org (Sohrab,Behnaz) Date: Fri Jan 9 13:09:34 2015 Subject: [Histonet] Automatic H & E slide stainer recommendations In-Reply-To: <42CE3C79-0F27-4B0D-9495-91BB153DA129@imagesbyhopper.com> References: <00f601d02adf$9aaf7cd0$d00e7670$@comcast.net>, <42CE3C79-0F27-4B0D-9495-91BB153DA129@imagesbyhopper.com> Message-ID: <92096F092E8CE54FBB5E272198FE5A7E0294F430@ahexcms001.ah.org> I agree ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histotech@imagesbyhopper.com [histotech@imagesbyhopper.com] Sent: Friday, January 09, 2015 3:37 AM To: ian bernard Cc: Subject: Re: [Histonet] Automatic H & E slide stainer recommendations I second the Leica XL. That stainer is a reliable workhorse. Sent from my iPhone On Jan 7, 2015, at 8:08 PM, ian bernard wrote: The Leica Autostainer XL has proven effective for our lab. IRB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, January 06, 2015 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H & E slide stainer recommendations Hello colleagues, I would appreciate any recommendations for an automatic slide stainer. It will primarily be used for H & E staining, not IHC. Thanks in advance, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> mercer.edu Fri Jan 9 13:39:03 2015 From: POWELL_SA <@t> mercer.edu (Shirley A. Powell) Date: Fri Jan 9 13:39:25 2015 Subject: [Histonet] And other crazy stuff. In-Reply-To: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE25C00E0AAB6@MERCERMAIL.MercerU.local> Orangutan testicle macro section and alligator jawbones, not my best work, very humbling, after 52 years in the business. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Tuesday, January 06, 2015 2:24 PM To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: And other crazy stuff. RE: [Histonet] cutting honey bees You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates.... Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com > From: rjr6@psu.edu > To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu > Date: Sat, 3 Jan 2015 23:15:33 +0000 > Subject: RE: [Histonet] cutting honey bees > CC: > > I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. > Roberta Horner > Penn State University > Animal Diagnostic Lab > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg > [classicdoc@gmail.com] > Sent: Saturday, January 03, 2015 6:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] cutting honey bees > > Has anyone had experience embedding and cutting honey bees. I am sure > there are some issues with the harder exoskeleton. Would that have to > be dissected away first. I am considering helping a student with a > science fair project on bees. > > Douglas Gregg > Veterianary pathologist > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HernandezJW <@t> uthscsa.edu Fri Jan 9 14:03:10 2015 From: HernandezJW <@t> uthscsa.edu (Hernandez, Jesus Willibaldo) Date: Fri Jan 9 14:03:17 2015 Subject: [Histonet] Bone Tissue in plastic embedding Message-ID: Dear Histonetter's, Happy new year to everyone! I come today with a couple of questions regarding human bone embedded in MMA: 1. What is a good technique to dissolve the MMA from the tissue section? Also any suggestions on how to stain with H&E? 2. After mounting my tissue sections (5-10microns) with Haupts adhesive, I noticed that the bone within the section was appearing opaque/darkened on the microscope? Any suggestions on what this might be? Thank you very much, -Jesse H. From tbraud <@t> holyredeemer.com Fri Jan 9 14:10:28 2015 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Fri Jan 9 14:11:19 2015 Subject: [Histonet] small biopsy protocol Message-ID: Hi Akemi ? For what it is worth. We used to cut small GI biopsies at 2 levels, 4 sections per level, per slide for H&E, plus one extra unstained between levels. We changed last year to 3 levels, 2 sections per level, all mounted on the same slide, plus one extra unstained with 2 sections from between levels 2 and 3 on gastrics for possible H.Pylori stain. For me, this is better because we are getting fewer requests for recuts. I Hope this helps Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Date: Fri, 9 Jan 2015 06:07:05 -0800 From: Eileen Akemi Allison Subject: [Histonet] Levels for Gastic and Esophogeal Bx's Good morning Histoland: Happy Friday! I believe this question was asked last year, but I would like to revisit this again. I have worked in a variety of laboratories in my career, both small and large. Most of my experience was in Oregon with large hospital laboratories and University institutions. Back then, we cut (6) levels on all our bx???s, and picked-up (2) sections on each level. My current facility is cutting (3) levels and picking up (1) section on each level. I would like to ask what everyone else is doing. The reason I am asking is because our pathology director is requesting a change in our protocol to cut only (2) levels and (1) section at each level. Sincerely, Akemi Allison BS, HT/HTL (ASCP) --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From hans <@t> histologistics.com Fri Jan 9 15:17:50 2015 From: hans <@t> histologistics.com (Hans B Snyder) Date: Fri Jan 9 15:17:59 2015 Subject: [Histonet] And other crazy stuff. In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25C00E0AAB6@MERCERMAIL.MercerU.local> References: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> <9BF995BC0E47744E9673A41486E24EE25C00E0AAB6@MERCERMAIL.MercerU.local> Message-ID: You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, I work in a research facility where I do everything. One day a botanist emailed me to cut pine on the cryostat and asked what the largest size should be. I told him 2 mm x 2mm and explained the process as he had no idea what I was going to do. I thought ok, what is pine? He brought 5 pieces of wood pieces (pine) and asked if they could be cut cross section at 10um with 5 sections per slide. Of course this is not something I have ever had expience in doing, and they are hard as a rock. The trick that ended up working, was to soak the wood in warm water for 30 minutes, embed in OCT and cut. After cutting the sections were all balled up, so I floated them on water and picked them up on the slide. The sections were still folded and looked horiffic in my mind but he loved them. He said was going to look at them under a Raman confocal and wanted to see the plant cell wall. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Fri, Jan 9, 2015 at 2:39 PM, Shirley A. Powell wrote: > Orangutan testicle macro section and alligator jawbones, not my best work, very humbling, after 52 years in the business. > Shirley > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Tuesday, January 06, 2015 2:24 PM > To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > Subject: And other crazy stuff. RE: [Histonet] cutting honey bees > > You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? > > For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. > > > Open the floodgates.... > > Tim Morken > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg > Sent: Tuesday, January 06, 2015 11:13 AM > To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > Subject: RE: [Histonet] cutting honey bees > > for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. > > Cheers, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > pruegghm@hotmail.com > > > >> From: rjr6@psu.edu >> To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu >> Date: Sat, 3 Jan 2015 23:15:33 +0000 >> Subject: RE: [Histonet] cutting honey bees >> CC: >> >> I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. >> Roberta Horner >> Penn State University >> Animal Diagnostic Lab >> ________________________________________ >> From: histonet-bounces@lists.utsouthwestern.edu >> [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg >> [classicdoc@gmail.com] >> Sent: Saturday, January 03, 2015 6:08 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] cutting honey bees >> >> Has anyone had experience embedding and cutting honey bees. I am sure >> there are some issues with the harder exoskeleton. Would that have to >> be dissected away first. I am considering helping a student with a >> science fair project on bees. >> >> Douglas Gregg >> Veterianary pathologist >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs <@t> gmail.com Fri Jan 9 15:20:43 2015 From: patpxs <@t> gmail.com (Paula Sicurello) Date: Fri Jan 9 15:20:48 2015 Subject: [Histonet] And other crazy stuff. In-Reply-To: <9BF995BC0E47744E9673A41486E24EE25C00E0AAB6@MERCERMAIL.MercerU.local> References: <761E2B5697F795489C8710BCC72141FF367E731F@ex07.net.ucsf.edu> <9BF995BC0E47744E9673A41486E24EE25C00E0AAB6@MERCERMAIL.MercerU.local> Message-ID: I forgot to mention: dirt Happy Friday Paula On Fri, Jan 9, 2015 at 2:39 PM, Shirley A. Powell wrote: > Orangutan testicle macro section and alligator jawbones, not my best work, > very humbling, after 52 years in the business. > Shirley > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy > Sent: Tuesday, January 06, 2015 2:24 PM > To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > Subject: And other crazy stuff. RE: [Histonet] cutting honey bees > > You crazy research people...OK, so what is the craziest thing you ever had > to cut, or were asked to cut? > > For me, not too bad, but embedding for EM and sectioning a single oocyte > that was nearly microscopic. I'll just say it took a LOT of thick sections > too face down to it without actually cutting through it. > > > Open the floodgates.... > > Tim Morken > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg > Sent: Tuesday, January 06, 2015 11:13 AM > To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu > Subject: RE: [Histonet] cutting honey bees > > for the whole bee I probably would process and embed it in glycol > methacrylate (gma) it is much harder and would give better sections, we > have done zebra fish and several other harder tissues including calcified > bone in GMA. > > Cheers, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > pruegghm@hotmail.com > > > > > From: rjr6@psu.edu > > To: classicdoc@gmail.com; histonet@lists.utsouthwestern.edu > > Date: Sat, 3 Jan 2015 23:15:33 +0000 > > Subject: RE: [Histonet] cutting honey bees > > CC: > > > > I sectioned and stained honey bee and yellow jacket stingers years ago. > They wanted to show the difference between the stingers. I wasn't sure > what to do so I processed and handled like everything else. I was able to > get some good sections. I put 6 stingers in each block and cut several > sections figuring there should be at least one good stinger in each block > and it worked. > > Roberta Horner > > Penn State University > > Animal Diagnostic Lab > > ________________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu > > [histonet-bounces@lists.utsouthwestern.edu] on behalf of Douglas Gregg > > [classicdoc@gmail.com] > > Sent: Saturday, January 03, 2015 6:08 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] cutting honey bees > > > > Has anyone had experience embedding and cutting honey bees. I am sure > > there are some issues with the harder exoskeleton. Would that have to > > be dissected away first. I am considering helping a student with a > > science fair project on bees. > > > > Douglas Gregg > > Veterianary pathologist > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From b-frederick <@t> northwestern.edu Fri Jan 9 17:05:51 2015 From: b-frederick <@t> northwestern.edu (Bernice Frederick) Date: Fri Jan 9 17:05:57 2015 Subject: [Histonet] Automatic H & E slide stainer recommendations In-Reply-To: <42CE3C79-0F27-4B0D-9495-91BB153DA129@imagesbyhopper.com> References: <00f601d02adf$9aaf7cd0$d00e7670$@comcast.net>, <42CE3C79-0F27-4B0D-9495-91BB153DA129@imagesbyhopper.com> Message-ID: <62C639732D3F274DACED033EBDF6ADAF2F1423E7@evcspmbx2.ads.northwestern.edu> I agree as well. The XL is a wokhorse. We have the transfer station and the CV5030 to go with it. Bernice Frederick (HTL) ASCP Senior Research Tech Pathology Core Facility Robert H. Lurie Cancer Center Northwestern University 710 North Fairbanks Court Olson Room 8421 Chicago, IL 60611 312-503-3723 b-frederick@northwestern.edu ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of histotech@imagesbyhopper.com [histotech@imagesbyhopper.com] Sent: Friday, January 09, 2015 5:37 AM To: ian bernard Cc: Subject: Re: [Histonet] Automatic H & E slide stainer recommendations I second the Leica XL. That stainer is a reliable workhorse. Sent from my iPhone On Jan 7, 2015, at 8:08 PM, ian bernard wrote: The Leica Autostainer XL has proven effective for our lab. IRB -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, January 06, 2015 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automatic H & E slide stainer recommendations Hello colleagues, I would appreciate any recommendations for an automatic slide stainer. It will primarily be used for H & E staining, not IHC. Thanks in advance, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmconway@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Fri Jan 9 17:09:11 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Jan 9 17:09:18 2015 Subject: [Histonet] PAS rx Message-ID: Some PAS procedures call for sulphurous rinses after the Schiffs reagent. >From your experience is there a noticeable difference if you do not do the rinse? Would it matter if you used sodium metabisulfite rather than potassium metabisulfite? Thank, Jennifer From Histotech <@t> imagesbyhopper.com Sat Jan 10 12:04:54 2015 From: Histotech <@t> imagesbyhopper.com (Michelle) Date: Sat Jan 10 12:05:54 2015 Subject: [Histonet] CLIA Inspection Question Message-ID: <034901d02cff$f2b73540$d8259fc0$@imagesbyhopper.com> Hi Histonetters! I am being asked to assist in CLIA inspection preparation for a brand new molecular lab. I have done many CAP inspections and love their checklists, but have never done one specific for CLIA before. Does CLIA offer similar checklists that we can work from? I have done some research on the net, but it can get so convoluted and confusing, I was hoping you might be able to help me navigate some of the waters. Thanks, in advance, Michelle From tmoore9k <@t> gmail.com Sun Jan 11 06:16:44 2015 From: tmoore9k <@t> gmail.com (Terry) Date: Sun Jan 11 06:16:51 2015 Subject: [Histonet] HT Certification Message-ID: <97C1BD7C-E9E1-4355-AD9B-26F1DCA708F9@gmail.com> I was told by my lab supervisor that it was against the law for me to work in the lab and not have my HT certification. I'm pretty sure this is not true (I work in OH in a hospital lab). I'm look for documentation so I can show her this is not true. Also wondering if the state does not require certification maybe it is a requirement for CAP certification of the lab. Sent from my iPad From a.prior <@t> tissueregenix.com Mon Jan 12 02:42:32 2015 From: a.prior <@t> tissueregenix.com (Andrew Prior) Date: Mon Jan 12 02:45:29 2015 Subject: [Histonet] Tendon processing Message-ID: Is anyone willing to share their wax processing schedule for tendon samples? I work on human and porcine tissues so the tendons are quite large , cutting transverse and longitudinal sections for H&E and PicroSirius Red staining. We have a protocol at the moment, but are looking to improve it. I am limited with the number of samples I have so can't do a large trial for optimising the process, so I was hoping my fellow histonetters could share their experience. Many thanks Andrew Andrew Prior Histologist Tissue Regenix Group York, UK YO10 5NY E-mail: a.prior@tissueregenix.com Website: www.tissueregenix.com From asanjeet <@t> yahoo.com Mon Jan 12 04:39:24 2015 From: asanjeet <@t> yahoo.com (Sanjeet) Date: Mon Jan 12 04:39:35 2015 Subject: [Histonet] Fite control Message-ID: <299B52E0-A744-4E8B-8ADC-7356F57AAB15@yahoo.com> Dear Histo folks, I am looking for fite control either block or slides Sent from my iPhone From Lesley.Bechtold <@t> jax.org Mon Jan 12 08:30:05 2015 From: Lesley.Bechtold <@t> jax.org (Lesley Bechtold) Date: Mon Jan 12 08:30:16 2015 Subject: [Histonet] Histology Service Manager Position - New! Message-ID: <08997064E2075247A4DD5A035DB51CDF657B87F1@jaxbhexms01.jax.org> Job Description We have an exciting opportunity for an experienced and energetic individual who is looking for a position where their work makes a difference in people's lives. At The Jackson Laboratory, we are leading the search for tomorrow's cures. As the Manager of the Histology Service, you will lead a team of dedicated and creative professional Histotechnologists and partner with our research staff as they focus on discovering solutions to genetically based diseases. Located in beautiful Bar Harbor, Maine, The Jackson Laboratory is a leading global genetics research institute. As we grow and expand, we are seeking talented individuals who are ready to meet this challenge. Key Responsibilities The Histology Service Manager: * Guides the Histology Team and oversees the day-to-day operations of the Histology Service. * Researches, recommends and introduces new technology and new procedures to meet the research goals of the scientific staff. * Captures critical metrics; analyzes and reports data. Utilizes findings to improve work environment and daily processes in the Histology Laboratory. * Collaborates with scientific staff on projects and ensures that their histological needs are met. * Establishes standards for histological processes and sets the precedent for best practices and high quality production. Required Knowledge, Skills and Abilities * Bachelor's degree in a biological science with HT (ASCP) or HTL (ASCP) certification and eight to ten (8-10) years of histology experience as a certified Histotechnologist. * Experience with histological techniques using animal models is desirable. * Certification in immunohistochemistry (QIHC) is required although equivalent experience (2 or more years) in immunohistochemical techniques may be considered. * Three to five (3-5) years of managing or supervisory experience. * Supervisory or lead experience in a clinical or pharmaceutical setting is desirable. Please refer to posting # 4752 on the careers link at www.jax.org Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From CArnold <@t> opko.com Mon Jan 12 09:34:34 2015 From: CArnold <@t> opko.com (Casey Arnold) Date: Mon Jan 12 10:36:55 2015 Subject: [Histonet] RE: HT certification Message-ID: <9ad3ad1f39cd4e05a9eeea0476fc4208@USNVLEXC01.opko.corp> It is not a CAP requirement unless it is required by your state. (I only know because we just had our CAP inspection). Check your state regulations, but the only states I know of off the top of my head that require certification are FL and NY. That being said, your certification is a good thing to have either way. I have access to some great study material if you are interested. Casey Arnold Histology Supervisor 1450 Elm Hill Pike Nashville, TN? 37210 Office:? 615.874.0410 Cell:? 615.714.2940 Fax:? 615.345.4595 carnold@opko.com www.opko.com NOTICE: This electronic mail message and any files transmitted with it are intended exclusively for the individual or entity to which it is addressed. The message, together with any attachment, may contain confidential and/or privileged information.? Any unauthorized review, use, printing, saving, copying, disclosure or distribution is strictly prohibited. If you have received this message in error, please immediately advise the sender by reply email and delete all copies. ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, January 11, 2015 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 134, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. CLIA Inspection Question (Michelle) 2. HT Certification (Terry) ---------------------------------------------------------------------- Message: 1 Date: Sat, 10 Jan 2015 13:04:54 -0500 From: "Michelle" Subject: [Histonet] CLIA Inspection Question To: Message-ID: <034901d02cff$f2b73540$d8259fc0$@imagesbyhopper.com> Content-Type: text/plain; charset="us-ascii" Hi Histonetters! I am being asked to assist in CLIA inspection preparation for a brand new molecular lab. I have done many CAP inspections and love their checklists, but have never done one specific for CLIA before. Does CLIA offer similar checklists that we can work from? I have done some research on the net, but it can get so convoluted and confusing, I was hoping you might be able to help me navigate some of the waters. Thanks, in advance, Michelle ------------------------------ Message: 2 Date: Sun, 11 Jan 2015 07:16:44 -0500 From: Terry Subject: [Histonet] HT Certification To: "histonet@lists.utsouthwestern.edu" Message-ID: <97C1BD7C-E9E1-4355-AD9B-26F1DCA708F9@gmail.com> Content-Type: text/plain; charset=us-ascii I was told by my lab supervisor that it was against the law for me to work in the lab and not have my HT certification. I'm pretty sure this is not true (I work in OH in a hospital lab). I'm look for documentation so I can show her this is not true. Also wondering if the state does not require certification maybe it is a requirement for CAP certification of the lab. Sent from my iPad ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 134, Issue 12 ***************************************** From aeck <@t> dh.org Mon Jan 12 07:13:43 2015 From: aeck <@t> dh.org (Eck, Allison) Date: Mon Jan 12 10:37:05 2015 Subject: [Histonet] job opening Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64A06ADF8@DH-MAIL01.dhorg.org> We currently have a part time opening for a histology technician in Doylestown, PA. This position is 4 hours a day, Monday - Friday. Job duties include: embedding, microtomy, special stains and IHC. For details or to apply, visit www.dh.org From jvickroy <@t> SpringfieldClinic.com Mon Jan 12 11:44:47 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Mon Jan 12 11:44:59 2015 Subject: [Histonet] Primera cassette and slide labelers Message-ID: <9B1A1501A800064397369BD8072E6BCA96B05A@E2K10DB.springfieldclinic.com> Sakura now carries the Primera made cassette and slide labelers. (Tissue Tek). Does anybody have any experience with these instruments? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From BZIMMERM <@t> gru.edu Mon Jan 12 11:44:33 2015 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Mon Jan 12 11:56:11 2015 Subject: [Histonet] SAVE THE DATE! APRIL 17TH - APRIL 19TH Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF8D9BF4@EX-MLB-03.ad.georgiahealth.edu> Happy Moanday! Save the date for HISTOPALOOZA 2015 ***************************************************************************************************************************** This year the Georgia Society for Histotechnology meeting will be held at the beautiful Legacy Lodge, Lake Lanier Islands. Stay tuned for more information. I wanted to give you a "heads up" on the dates so you can begin to plan a getaway weekend. I've seen the lodge firsthand and it's beautiful. It will be a great location for continuing education as well as mental health therapy. And, boy I need a truckload of that stuff. Hehe Billie Zimmerman MT(ASCP)QIHC Secretary GSH From mjones <@t> metropath.com Mon Jan 12 12:04:07 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Mon Jan 12 12:04:22 2015 Subject: [Histonet] Primera cassette and slide labelers Message-ID: I went and saw the Primera cassette printer and played with it at the NSH. Beautiful machine, lots of fun lights! The cassette is raised up - rather than dropping down. Lots of little moving parts. I went with the General Data new 8 hopper version, because I was a little nervous about all of the moving parts and it took 11 seconds to print one cassette! Prints beautifully though, ( the Primera) and can use different colors of ink, different directions of print and on the side or front of the cassette. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 1/12/15, 10:44 AM, "Vickroy, James" wrote: > >Sakura now carries the Primera made cassette and slide labelers. (Tissue >Tek). Does anybody have any experience with these instruments? > >Jim > >Jim Vickroy >Histology Manager >Springfield Clinic, Main Campus, East Building >1025 South 6th Street >Springfield, Illinois 62703 >Office: 217-528-7541, Ext. 15121 >Email: >jvickroy@SpringfieldClinic.com > > >This electronic message contains information from Springfield Clinic, LLP >that may be confidential, privileged, and/or sensitive. This information >is intended for the use of the individual(s) or entity(ies) named above. >If you are not the intended recipient, be aware that disclosure, copying, >distribution, or action taken on the contents of this information is >strictly prohibited. If you have received this electronic message in >error, please notify the sender immediately, by electronic mail, so that >arrangements may be made for the retrieval of this electronic message. >Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cdemarinis <@t> SARATOGACARE.ORG Mon Jan 12 12:29:23 2015 From: cdemarinis <@t> SARATOGACARE.ORG (Demarinis, Carolyn) Date: Mon Jan 12 12:29:28 2015 Subject: [Histonet] Pathology Messages Message-ID: <1C75A843982A7B44BB368A3CC946ABCA0108ED84BB@SHEXCHMBX01.SARAHOSP.ORG> We have Meditech Magic 5.66 and I would like to initiate an electronic process for pathologists to order special stains, recuts, etc. Does anyone have experience with PATHOLOGY SPECIMEN MESSAGES, and if so, will it work to meet our needs? Presently the pathologists are using pathology templates that they complete on the computer in Microsoft Word, and they print a hard copy and bring it to Pathology. Thank you. This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Saratoga Hospital immediately by e-mail at privacy@saratogacare.org and destroy all copies of this communication and any attachments. From hmyers-bird <@t> rrmc.org Mon Jan 12 12:33:54 2015 From: hmyers-bird <@t> rrmc.org (Heidi Myers-Bird) Date: Mon Jan 12 12:34:01 2015 Subject: [Histonet] ISH Message-ID: I am looking for any info/webinar/educational material for in situ hybridization (ISH). We would like to bring this into our lab on the Ventana BenchMark Ultra. Thanks, Heidi Heidi Bird, HT (ASCP) Histotechnologist Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 Phone: 802.747.1791 Fax:802.747.6525 email: hmyers-bird@rrmc.org Our Vision: To be the Best Community Hospital and Health System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You From bszpunar <@t> umail.iu.edu Mon Jan 12 12:45:47 2015 From: bszpunar <@t> umail.iu.edu (Bryan Szpunar) Date: Mon Jan 12 12:45:52 2015 Subject: [Histonet] RE: HT certification Message-ID: Casey is correct--unless you live/work in a state that has its own requirements, there is no national requirement to be HT/HTL certified. That said, employers can and do enforce their own requirements, and there is also the separate subject of being credentialed for the testing which you are performing (i.e. grossing). At the end of the day though, it is the employers prerogative on requiring certification in lieu of any state laws. Hope that helps, Bryan From bszpunar <@t> umail.iu.edu Mon Jan 12 12:50:40 2015 From: bszpunar <@t> umail.iu.edu (Bryan Szpunar) Date: Mon Jan 12 12:50:54 2015 Subject: [Histonet] RE: CLIA Inspection Question Message-ID: Hi Michelle, Yes, the CLIA regs are such a joy to read aren't they? To my knowledge there is not a plain English version of them (less all the "if, or, or, and..." fun on every line). However, the CAP is basically enforcing CLIA regulations, so if it were me, I would start with the CAP molecular checklist and go from there, delving into the CLIA regs for clarification on details. Bryan From jvickroy <@t> SpringfieldClinic.com Mon Jan 12 12:57:50 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Mon Jan 12 12:57:56 2015 Subject: [Histonet] Cassette printer and slide or lable printers Message-ID: <9B1A1501A800064397369BD8072E6BCA96B0D7@E2K10DB.springfieldclinic.com> Comparing different products. Currently we have Cyberpath as our anatomic pathology system. I am trying to purchase a cassette printer and slide printers and am trying to figure out the added expenses for connecting these instruments to the CyberPath system. I have an email out to CyperPath but also wondered if anyone can share their experiences when they connected their cassette printers and slide or label printers with me. I suspect I have to have an interface from Cyberpath to the printers but will I need to purchase anything on the cassette or slide printer side. This is definitely not my strong suit and any advice would be greatly appreciated. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From rsrichmond <@t> gmail.com Mon Jan 12 13:26:06 2015 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Mon Jan 12 13:26:10 2015 Subject: [Histonet] Fite control (for Myco. leprae) Message-ID: Sanjeet asked about a "Fite control" - I suppose Mycobacterium leprae infected tissue for the Fite-Faraco modification of the visible-light acid-fast stain. It actually is necessary to use Myco. leprae (which of course is unculturable) rather than Myco. tuberculosis when staining for lepra bacilli. I've asked this question several times over the years and never got the faintest reply, but - the North American armadillo (Dasypus novemcinctus) is easily infected with human leprosy, and in fact is naturally infected in Louisiana. One lepradillo could I think provide control material for the entire planet. Has such material ever become available? Two more questions: Can you perform the fluorescent (auramine O) acid-fast stain with the Fite modification? And - do you also need a different control for Mycobacterium avium-intracellulare (MAI)? Bob Richmond Samurai Pathologist Maryville TN From vgarcia <@t> auroradx.com Mon Jan 12 13:29:23 2015 From: vgarcia <@t> auroradx.com (Garcia, Vanessa) Date: Mon Jan 12 13:29:40 2015 Subject: [Histonet] RE: ISH In-Reply-To: References: Message-ID: <33A389EAD3D6A74F8A47FD7571071E840A3F23DE@CPEXCH02.cunninghampathology.com> Your ventana rep should have some ppts that will give you info and a little background on ISH and use on the ventanas. Especially if you are considering bringing it on. Vanessa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heidi Myers-Bird Sent: Monday, January 12, 2015 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ISH I am looking for any info/webinar/educational material for in situ hybridization (ISH). We would like to bring this into our lab on the Ventana BenchMark Ultra. Thanks, Heidi Heidi Bird, HT (ASCP) Histotechnologist Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 Phone: 802.747.1791 Fax:802.747.6525 email: hmyers-bird@rrmc.org Our Vision: To be the Best Community Hospital and Health System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, contains confidential and/or privileged information and is for the sole use of the intended recipient(s). Any unauthorized review, use, disclosure or distribution is strictly prohibited. If you are not the intended recipient and have received this e-mail in error, please contact the sender immediately by reply e-mail and destroy all copies of the original message. Thank you. From Cnituda <@t> nvdermatology.com Mon Jan 12 13:27:35 2015 From: Cnituda <@t> nvdermatology.com (Carl Nituda) Date: Mon Jan 12 13:29:56 2015 Subject: [Histonet] RE: HT certification In-Reply-To: <9ad3ad1f39cd4e05a9eeea0476fc4208@USNVLEXC01.opko.corp> References: <9ad3ad1f39cd4e05a9eeea0476fc4208@USNVLEXC01.opko.corp> Message-ID: Nevada requires state license too for Histotechnologist. CN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Casey Arnold Sent: Monday, January 12, 2015 7:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: HT certification It is not a CAP requirement unless it is required by your state. (I only know because we just had our CAP inspection). Check your state regulations, but the only states I know of off the top of my head that require certification are FL and NY. That being said, your certification is a good thing to have either way. I have access to some great study material if you are interested. Casey Arnold Histology Supervisor 1450 Elm Hill Pike Nashville, TN? 37210 Office:? 615.874.0410 Cell:? 615.714.2940 Fax:? 615.345.4595 carnold@opko.com www.opko.com NOTICE: This electronic mail message and any files transmitted with it are intended exclusively for the individual or entity to which it is addressed. The message, together with any attachment, may contain confidential and/or privileged information.? Any unauthorized review, use, printing, saving, copying, disclosure or distribution is strictly prohibited. If you have received this message in error, please immediately advise the sender by reply email and delete all copies. ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, January 11, 2015 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 134, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. CLIA Inspection Question (Michelle) 2. HT Certification (Terry) ---------------------------------------------------------------------- Message: 1 Date: Sat, 10 Jan 2015 13:04:54 -0500 From: "Michelle" Subject: [Histonet] CLIA Inspection Question To: Message-ID: <034901d02cff$f2b73540$d8259fc0$@imagesbyhopper.com> Content-Type: text/plain; charset="us-ascii" Hi Histonetters! I am being asked to assist in CLIA inspection preparation for a brand new molecular lab. I have done many CAP inspections and love their checklists, but have never done one specific for CLIA before. Does CLIA offer similar checklists that we can work from? I have done some research on the net, but it can get so convoluted and confusing, I was hoping you might be able to help me navigate some of the waters. Thanks, in advance, Michelle ------------------------------ Message: 2 Date: Sun, 11 Jan 2015 07:16:44 -0500 From: Terry Subject: [Histonet] HT Certification To: "histonet@lists.utsouthwestern.edu" Message-ID: <97C1BD7C-E9E1-4355-AD9B-26F1DCA708F9@gmail.com> Content-Type: text/plain; charset=us-ascii I was told by my lab supervisor that it was against the law for me to work in the lab and not have my HT certification. I'm pretty sure this is not true (I work in OH in a hospital lab). I'm look for documentation so I can show her this is not true. Also wondering if the state does not require certification maybe it is a requirement for CAP certification of the lab. Sent from my iPad ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 134, Issue 12 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From emd1d <@t> yahoo.com Mon Jan 12 14:57:13 2015 From: emd1d <@t> yahoo.com (Ellen) Date: Mon Jan 12 14:57:17 2015 Subject: [Histonet] Block Counts Message-ID: <204FC2F0-9797-4059-9144-C99AE90A8D9C@yahoo.com> I'm looking for raw data on time studies that directly deal with the number of blocks a PA can produce in a day and how many a histo tech can cut a day. Thanks Sent from my iPhone From TGoins <@t> mt.gov Mon Jan 12 15:58:06 2015 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Mon Jan 12 16:00:51 2015 Subject: [Histonet] Block Counts In-Reply-To: <204FC2F0-9797-4059-9144-C99AE90A8D9C@yahoo.com> References: <204FC2F0-9797-4059-9144-C99AE90A8D9C@yahoo.com> Message-ID: Depends on the type of tissue. Depends on the length of time your "day" is for a repetitive task. Assigning an arbitrary number is counterproductive. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen Sent: Monday, January 12, 2015 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Counts I'm looking for raw data on time studies that directly deal with the number of blocks a PA can produce in a day and how many a histo tech can cut a day. Thanks Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Mon Jan 12 16:46:23 2015 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Mon Jan 12 16:46:34 2015 Subject: [Histonet] Block Counts In-Reply-To: References: <204FC2F0-9797-4059-9144-C99AE90A8D9C@yahoo.com> Message-ID: I have always found there are so many variables that having an expectation of setting a set number is not always possible. I feel they should be compared to their own standards and not of their co-workers. If they can cut or embed a set number on a particular than they should maintain or gradually increase (for newer techs) over time. . I have seen where in one day they cut so many blocks when the work load mandates it but on a slower day it takes them just as much time to cut half as many blocks. The work pace should be at their comfort level but should be a standard rate. Diana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: January-12-15 4:58 PM To: Ellen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block Counts Depends on the type of tissue. Depends on the length of time your "day" is for a repetitive task. Assigning an arbitrary number is counterproductive. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen Sent: Monday, January 12, 2015 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Counts I'm looking for raw data on time studies that directly deal with the number of blocks a PA can produce in a day and how many a histo tech can cut a day. Thanks Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Mon Jan 12 17:01:26 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Mon Jan 12 17:05:07 2015 Subject: [Histonet] Block Counts In-Reply-To: References: <204FC2F0-9797-4059-9144-C99AE90A8D9C@yahoo.com> Message-ID: <761E2B5697F795489C8710BCC72141FF367EA257@ex07.net.ucsf.edu> I agree with Diana, I found we had over a dozen different task mixes in a given day for various techs. That includes mix of block types (bx, extensive lists of stain requests per block, single H&E, recuts, mega block, research cases), other tasks (Staining, speicals, ihc, tissue processor . Grossing would be even more complicated. Instead of a per-day count, use per hour or per two hours - Some period when the person is concentrating on a single task without interruption. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Monday, January 12, 2015 2:46 PM To: 'Goins, Tresa'; Ellen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block Counts I have always found there are so many variables that having an expectation of setting a set number is not always possible. I feel they should be compared to their own standards and not of their co-workers. If they can cut or embed a set number on a particular than they should maintain or gradually increase (for newer techs) over time. . I have seen where in one day they cut so many blocks when the work load mandates it but on a slower day it takes them just as much time to cut half as many blocks. The work pace should be at their comfort level but should be a standard rate. Diana -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: January-12-15 4:58 PM To: Ellen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Block Counts Depends on the type of tissue. Depends on the length of time your "day" is for a repetitive task. Assigning an arbitrary number is counterproductive. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen Sent: Monday, January 12, 2015 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Block Counts I'm looking for raw data on time studies that directly deal with the number of blocks a PA can produce in a day and how many a histo tech can cut a day. Thanks Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Mon Jan 12 17:17:53 2015 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Mon Jan 12 17:18:03 2015 Subject: [Histonet] Block Counts In-Reply-To: <761E2B5697F795489C8710BCC72141FF367EA257@ex07.net.ucsf.edu> References: <204FC2F0-9797-4059-9144-C99AE90A8D9C@yahoo.com> <761E2B5697F795489C8710BCC72141FF367EA257@ex07.net.ucsf.edu> Message-ID: This discussion is exactly why we do not count blocks at microtomy, only slides. Counting slides is the equalizer for multiple levels and slides per block. Count the most appropriate unit at a task (i. e. slides at microtomy, blocks at embedding, specimens at grossing, specimens at accession inc) that allows you to set work pace and creates a corresponding quality measure. William DeSalvo william.desalvo@sonoraquest.com 602-768-3692 Sent from my iPhone > On Jan 12, 2015, at 4:05 PM, Morken, Timothy wrote: > > I agree with Diana, I found we had over a dozen different task mixes in a given day for various techs. That includes mix of block types (bx, extensive lists of stain requests per block, single H&E, recuts, mega block, research cases), other tasks (Staining, speicals, ihc, tissue processor . Grossing would be even more complicated. Instead of a per-day count, use per hour or per two hours - Some period when the person is concentrating on a single task without interruption. > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig > Sent: Monday, January 12, 2015 2:46 PM > To: 'Goins, Tresa'; Ellen; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Block Counts > > I have always found there are so many variables that having an expectation of setting a set number is not always possible. I feel they should be compared to their own standards and not of their co-workers. If they can cut or embed a set number on a particular than they should maintain or gradually increase (for newer techs) over time. . > I have seen where in one day they cut so many blocks when the work load mandates it but on a slower day it takes them just as much time to cut half as many blocks. The work pace should be at their comfort level but should be a standard rate. > > Diana > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa > Sent: January-12-15 4:58 PM > To: Ellen; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Block Counts > > Depends on the type of tissue. > Depends on the length of time your "day" is for a repetitive task. > Assigning an arbitrary number is counterproductive. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Sent: Monday, January 12, 2015 1:57 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Block Counts > > I'm looking for raw data on time studies that directly deal with the number of blocks a PA can produce in a day and how many a histo tech can cut a day. > > > Thanks > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpodawiltz <@t> lrgh.org Tue Jan 13 05:07:16 2015 From: tpodawiltz <@t> lrgh.org (Podawiltz, Thomas) Date: Tue Jan 13 05:07:33 2015 Subject: [Histonet] Block Counts In-Reply-To: References: <204FC2F0-9797-4059-9144-C99AE90A8D9C@yahoo.com> <761E2B5697F795489C8710BCC72141FF367EA257@ex07.net.ucsf.edu> Message-ID: <38667E7FB77ECD4E91BFAEB8D986386326162EEB81@LRGHEXVS1.practice.lrgh.org> I have never really cared how fast or how many blocks can be embedded or cut per hour. I have always focused on the quality. In a hospital setting there is too much variety to have set numbers. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Monday, January 12, 2015 6:18 PM To: Morken, Timothy Cc: histonet@lists.utsouthwestern.edu; Goins, Tresa; Diana McCaig; Ellen Subject: Re: [Histonet] Block Counts This discussion is exactly why we do not count blocks at microtomy, only slides. Counting slides is the equalizer for multiple levels and slides per block. Count the most appropriate unit at a task (i. e. slides at microtomy, blocks at embedding, specimens at grossing, specimens at accession inc) that allows you to set work pace and creates a corresponding quality measure. William DeSalvo william.desalvo@sonoraquest.com 602-768-3692 Sent from my iPhone > On Jan 12, 2015, at 4:05 PM, Morken, Timothy wrote: > > I agree with Diana, I found we had over a dozen different task mixes in a given day for various techs. That includes mix of block types (bx, extensive lists of stain requests per block, single H&E, recuts, mega block, research cases), other tasks (Staining, speicals, ihc, tissue processor . Grossing would be even more complicated. Instead of a per-day count, use per hour or per two hours - Some period when the person is concentrating on a single task without interruption. > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special > Studies UC San Francisco Medical Center San Francisco, CA > > CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana > McCaig > Sent: Monday, January 12, 2015 2:46 PM > To: 'Goins, Tresa'; Ellen; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Block Counts > > I have always found there are so many variables that having an expectation of setting a set number is not always possible. I feel they should be compared to their own standards and not of their co-workers. If they can cut or embed a set number on a particular than they should maintain or gradually increase (for newer techs) over time. . > I have seen where in one day they cut so many blocks when the work load mandates it but on a slower day it takes them just as much time to cut half as many blocks. The work pace should be at their comfort level but should be a standard rate. > > Diana > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, > Tresa > Sent: January-12-15 4:58 PM > To: Ellen; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Block Counts > > Depends on the type of tissue. > Depends on the length of time your "day" is for a repetitive task. > Assigning an arbitrary number is counterproductive. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen > Sent: Monday, January 12, 2015 1:57 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Block Counts > > I'm looking for raw data on time studies that directly deal with the number of blocks a PA can produce in a day and how many a histo tech can cut a day. > > > Thanks > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From mchilds2010 <@t> hotmail.com Tue Jan 13 05:48:47 2015 From: mchilds2010 <@t> hotmail.com (Hotmail) Date: Tue Jan 13 05:50:07 2015 Subject: [Histonet] Needed! Presenters for FSH 2015 Message-ID: Hi histonetters! The 2015 Florida Society Meeting will be held May 15-17 in Orlando. I know we have some great presenters out there who want to visit the beautiful city of Orlando! Maybe someone out there wants to get started with presenting? (Here's that nudge you've been waiting for...) What a great setting and group we have here in Florida to help accommodate the best of speakers-new and experienced. Interested? Submit your abstracts to me at mchilds2010@hotmail.com by Feb. 1. Come join us for sun, fun and learning! Looking forward to what you have to share with us here in Florida. Happy New Year to you all! Michelle Foster FSH Vice President Sent from my iPhone From emd1d <@t> yahoo.com Tue Jan 13 07:16:48 2015 From: emd1d <@t> yahoo.com (Ellen) Date: Tue Jan 13 07:16:59 2015 Subject: [Histonet] Block Counts In-Reply-To: <38667E7FB77ECD4E91BFAEB8D986386326162EEB81@LRGHEXVS1.practice.lrgh.org> References: <204FC2F0-9797-4059-9144-C99AE90A8D9C@yahoo.com> <761E2B5697F795489C8710BCC72141FF367EA257@ex07.net.ucsf.edu> <38667E7FB77ECD4E91BFAEB8D986386326162EEB81@LRGHEXVS1.practice.lrgh.org> Message-ID: <59B6B675-2F98-4A18-B698-FDA2DD8DCAF8@yahoo.com> I feel the same way. I've been an Histo tech, AP manager, now a PA for the last 13 years and I can't get these people to understand. However I'm in a hospital system (4 hospitals) a lab director ( no pathology background) that is assigning works units to blocks as bill able units. We have 2 PA's, 7 Histo techs, 8 pathologist, 2 cyto techs. The director ( a pathologist) is trying to implement 200 blocks per PA. The problem is that 80% of our cases are complex, we average 2 whipples every other day, a truck load of mastectomies etc., Sent from my iPhone > On Jan 13, 2015, at 5:07 AM, "Podawiltz, Thomas" wrote: > > I have never really cared how fast or how many blocks can be embedded or cut per hour. I have always focused on the quality. In a hospital setting there is too much variety to have set numbers. > > > > Tom Podawiltz HT (ASCP) > Histology Section Head > LRGHealthcare > Laconia, NH 03246 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO > Sent: Monday, January 12, 2015 6:18 PM > To: Morken, Timothy > Cc: histonet@lists.utsouthwestern.edu; Goins, Tresa; Diana McCaig; Ellen > Subject: Re: [Histonet] Block Counts > > This discussion is exactly why we do not count blocks at microtomy, only slides. Counting slides is the equalizer for multiple levels and slides per block. Count the most appropriate unit at a task (i. e. slides at microtomy, blocks at embedding, specimens at grossing, specimens at accession inc) that allows you to set work pace and creates a corresponding quality measure. > > William DeSalvo > william.desalvo@sonoraquest.com > 602-768-3692 > Sent from my iPhone > >> On Jan 12, 2015, at 4:05 PM, Morken, Timothy wrote: >> >> I agree with Diana, I found we had over a dozen different task mixes in a given day for various techs. That includes mix of block types (bx, extensive lists of stain requests per block, single H&E, recuts, mega block, research cases), other tasks (Staining, speicals, ihc, tissue processor . Grossing would be even more complicated. Instead of a per-day count, use per hour or per two hours - Some period when the person is concentrating on a single task without interruption. >> >> Tim Morken >> Supervisor, Histology, Electron Microscopy and Neuromuscular Special >> Studies UC San Francisco Medical Center San Francisco, CA >> >> CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana >> McCaig >> Sent: Monday, January 12, 2015 2:46 PM >> To: 'Goins, Tresa'; Ellen; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Block Counts >> >> I have always found there are so many variables that having an expectation of setting a set number is not always possible. I feel they should be compared to their own standards and not of their co-workers. If they can cut or embed a set number on a particular than they should maintain or gradually increase (for newer techs) over time. . >> I have seen where in one day they cut so many blocks when the work load mandates it but on a slower day it takes them just as much time to cut half as many blocks. The work pace should be at their comfort level but should be a standard rate. >> >> Diana >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, >> Tresa >> Sent: January-12-15 4:58 PM >> To: Ellen; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Block Counts >> >> Depends on the type of tissue. >> Depends on the length of time your "day" is for a repetitive task. >> Assigning an arbitrary number is counterproductive. >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ellen >> Sent: Monday, January 12, 2015 1:57 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Block Counts >> >> I'm looking for raw data on time studies that directly deal with the number of blocks a PA can produce in a day and how many a histo tech can cut a day. >> >> >> Thanks >> >> Sent from my iPhone >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > THIS MESSAGE IS CONFIDENTIAL. > This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. From tjey <@t> hotmail.com Tue Jan 13 07:46:14 2015 From: tjey <@t> hotmail.com (Tanya Ewing-Finchem) Date: Tue Jan 13 07:46:22 2015 Subject: [Histonet] FFPE Tissue as a bio-hazard Message-ID: I am looking for documentation that talks about FFPE tissue as it relates to bio-hazards in the lab. When and where does tissue change from a bio-hazard to non bio-hazard. Needing to present to our safety department. They are ready to put us on lock down ;0/. Thanks for your help! Sent from my iPad From Erin.Martin <@t> ucsf.edu Tue Jan 13 08:13:17 2015 From: Erin.Martin <@t> ucsf.edu (Martin, Erin) Date: Tue Jan 13 08:13:24 2015 Subject: [Histonet] Re: Block counts Message-ID: <24B7B291CC88D04AB663958E77A1F59D1E0CFA@ex09.net.ucsf.edu> Good morning! I agree with some of the other comments - "per day" is too variable because of tissue type, overall volume, etc. We use hourly average to try to keep everyone in the same range but still leave room for individual abilities. Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. From mjones <@t> metropath.com Tue Jan 13 08:25:52 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Tue Jan 13 08:26:09 2015 Subject: [Histonet] Re: Block counts Message-ID: I agree- we use the product as the measure: slides for microtomy, specimens/hr for gross, etc. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 1/13/15, 7:13 AM, "Martin, Erin" wrote: >Good morning! I agree with some of the other comments - "per day" is too >variable because of tissue type, overall volume, etc. We use hourly >average to try to keep everyone in the same range but still leave room >for individual abilities. > > > >Erin > > > >Erin Martin, Histology Supervisor >UCSF Dermatopathology Service >415-353-7248 > >Confidentiality Notice >The information transmitted is intended only for the person or entity to >which it is addressed and may contain confidential and/or priviledged >material. Any review, retransmission, dissemination or other use of, or >taking of any action in reliance upon, this information by persons or >entities other than the intended recipient is prohibited. If you receive >this in error, please contact the sender and delete the material from any >computer. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From klaus.dern44 <@t> gmail.com Tue Jan 13 08:49:06 2015 From: klaus.dern44 <@t> gmail.com (Klaus Dern) Date: Tue Jan 13 08:49:11 2015 Subject: [Histonet] THICK AND THIN SECTIONS ? Message-ID: If you are using one of the following microtomes and the advance mechanism is worn out. ( too much play between spindle and spindle nut ) REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA/JUNG 2035 LEICA - CM 1850 Cryostat SAKURA SRM 200 You could be faced with purchasing a new Microtome. ( No parts availability ) Rather than replacing these excellent Instruments, I have a PERMANENT solution to fix this problem. For Information, contact: Klaus Dern Phone: 706 635-8840 E-Mail: klaus.dern44@gmail.com From jvickroy <@t> SpringfieldClinic.com Tue Jan 13 09:44:25 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Tue Jan 13 09:44:34 2015 Subject: [Histonet] Cassette lableler and slide labeler Message-ID: <9B1A1501A800064397369BD8072E6BCA96B378@E2K10DB.springfieldclinic.com> I didn't plan on this however wondered if anyone had a General Data system for making cassettes but a different setup for making slides, such as a Thermo Slidemate. I suspect there are issues with the LIS system, barcoding, etc. Am I wrong? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From Timothy.Morken <@t> ucsf.edu Tue Jan 13 09:51:14 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Tue Jan 13 09:51:24 2015 Subject: [Histonet] FFPE Tissue as a bio-hazard In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF367EA3F0@ex07.net.ucsf.edu> Tanya, in our institution we don't treat intact paraffin blocks as a biohazard, but we do collect all trimmings from the microtomes and put in red biohazard bags. The reasoning is that no one outside the lab knows what this stuff is so we err on the side of the safety, or perceived safety, of those downstream in waste cycle. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tanya Ewing-Finchem Sent: Tuesday, January 13, 2015 5:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FFPE Tissue as a bio-hazard I am looking for documentation that talks about FFPE tissue as it relates to bio-hazards in the lab. When and where does tissue change from a bio-hazard to non bio-hazard. Needing to present to our safety department. They are ready to put us on lock down ;0/. Thanks for your help! Sent from my iPad _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Tue Jan 13 10:06:57 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Tue Jan 13 10:07:15 2015 Subject: [Histonet] Cassette lableler and slide labeler Message-ID: I am very interested in the answer to this questions, as we are in the process of trying to procure a General Data cassette printer, but I am not sure I will try for the slide printers. I would think you could map any instrument to your LIS, and barcode readers would read any 2-D barcode - the issue might be compiling data for stats? Thanks for asking this question. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 1/13/15, 8:44 AM, "Vickroy, James" wrote: > >I didn't plan on this however wondered if anyone had a General Data >system for making cassettes but a different setup for making slides, such >as a Thermo Slidemate. > >I suspect there are issues with the LIS system, barcoding, etc. Am I >wrong? > >Jim > >Jim Vickroy >Histology Manager >Springfield Clinic, Main Campus, East Building >1025 South 6th Street >Springfield, Illinois 62703 >Office: 217-528-7541, Ext. 15121 >Email: >jvickroy@SpringfieldClinic.com > > >This electronic message contains information from Springfield Clinic, LLP >that may be confidential, privileged, and/or sensitive. This information >is intended for the use of the individual(s) or entity(ies) named above. >If you are not the intended recipient, be aware that disclosure, copying, >distribution, or action taken on the contents of this information is >strictly prohibited. If you have received this electronic message in >error, please notify the sender immediately, by electronic mail, so that >arrangements may be made for the retrieval of this electronic message. >Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Toni.McDaniel <@t> ky.gov Tue Jan 13 12:53:42 2015 From: Toni.McDaniel <@t> ky.gov (McDaniel, Toni (Justice)) Date: Tue Jan 13 12:54:30 2015 Subject: [Histonet] influenza IHC Message-ID: <5D09742D8BBF254588268A6C35B92CE2633C5A51@AGEXM01.eas.ds.ky.gov> I was approached by one of my pathologist with this question. Does anyone perform influenza IHC?? Contact me if you do or have any info referencing that specific testing ability. Toni McDaniel-Martin Forensic Histotech Specialist HT, ACSP University of Louisville Forensic Pathology 810 Barrett Ave Louisville KY 40204 Office: 502-852-5587 From akelley <@t> path.wustl.edu Tue Jan 13 13:10:04 2015 From: akelley <@t> path.wustl.edu (Kelley, Amanda) Date: Tue Jan 13 13:10:22 2015 Subject: [Histonet] RE: influenza IHC In-Reply-To: <5D09742D8BBF254588268A6C35B92CE2633C5A51@AGEXM01.eas.ds.ky.gov> References: <5D09742D8BBF254588268A6C35B92CE2633C5A51@AGEXM01.eas.ds.ky.gov> Message-ID: Email Dr. Richard Cartun He specializes in infectious disease IHC. Richard.Cartun@hhchealth.org Amanda Kelley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of McDaniel, Toni (Justice) Sent: Tuesday, January 13, 2015 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] influenza IHC I was approached by one of my pathologist with this question. Does anyone perform influenza IHC?? Contact me if you do or have any info referencing that specific testing ability. Toni McDaniel-Martin Forensic Histotech Specialist HT, ACSP University of Louisville Forensic Pathology 810 Barrett Ave Louisville KY 40204 Office: 502-852-5587 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. From Wanda.Borowicz <@t> sanfordhealth.org Tue Jan 13 15:02:53 2015 From: Wanda.Borowicz <@t> sanfordhealth.org (Borowicz,Wanda) Date: Tue Jan 13 15:03:39 2015 Subject: [Histonet] How are you applying this? Message-ID: <4B322E43736F1C48B60E560707CEF2B9011701FDCE@FAREXMBX4.sanfordhealth.org> Hi All, Below is a copy of the revised COM.40000 CAP checklist question. Now that Anatomic Pathology is having to comply with the All Common checklist, how are you applying this to your Immunohistochemistry ASR?s which are not FDA approved. We do new antibody validation and parallel testing with new lot numbers and clones. Is this enough? Can?t really see how the highlighted area pertains to this. Any advice would be appreciated. Thank. REVISED** 04/21/2014 COM.40000 Method Validation/Verification Approval Phase II There is a summary statement, signed by the laboratory director (or designee who meets CAP director qualifications) prior to use in patient testing, documenting evaluation of validation/verification studies and approval of each test for clinical use. NOTE: This checklist item is applicable only to tests implemented after June 15, 2009. The summary statement must include a written assessment of the validation/verification study, including the acceptability of the data. The summary must also include a statement approving the test for clinical use with the approval signature such as, "This validation study has been reviewed, and the performance of the method is considered acceptable for patient testing." For an FDA-cleared/approved test, a summary of the verification data must address analytic performance specifications, including analytic accuracy, precision, interferences, and reportable range, as applicable. In addition, for modified FDA-cleared/approved tests or LDTs, the summary must address analytical sensitivity, analytical specificity and any other parameter that is considered important to assure that the analytical performance of a test (e.g. specimen stability, reagent stability, linearity, carryover, and cross-contamination, etc.), as appropriate and applicable. If the laboratory makes clinical claims about its tests, the summary must address the validation of these claims. See the Method Performance Specifications section for details concerning validation/verification. Evidence of Compliance: ? Summary of validation/verification studies with review and approval REFERENCES 1) Lawrence Jennings, Vivianna M. Van Deerlin, Margaret L. Gulley (2009) Recommended Principles and Practices for Validating Clinical Molecular Pathology Tests. Archives of Pathology & Laboratory Medicine: Vol. 133, No. 5, pp. 743-755 Wanda Borowicz HT(ASCP) Histology Supervisor Sanford Health North 1720 S. University Dr. Route 1902 Fargo, ND 58103 Ph-701 417 4930 Fax-701 417 4399 wanda.borowicz@sanfordhealth.org ----------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain privileged and confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Timothy.Morken <@t> ucsf.edu Tue Jan 13 15:19:04 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Tue Jan 13 15:19:15 2015 Subject: [Histonet] RE: How are you applying this? In-Reply-To: <4B322E43736F1C48B60E560707CEF2B9011701FDCE@FAREXMBX4.sanfordhealth.org> References: <4B322E43736F1C48B60E560707CEF2B9011701FDCE@FAREXMBX4.sanfordhealth.org> Message-ID: <761E2B5697F795489C8710BCC72141FF367EA5B7@ex07.net.ucsf.edu> Yes, the ASR validation wording is easily applicable to clinical chemistry because they work with known amounts of an anylate but not exactly applicable to qualitative IHC without some explanation of the terms. I gave an NSH workshop addressing this and it is not easy to put in a quick email . Basically for ASR's you need to do a full validation of the antibody. You need to design the validation, document it, and then document that the antibody works as you expected. This takes some literature reading. By law a vendor cannot give you any information about how to do the test. That is all on the lab. The references below will give you what you need to know. The most recent recommendations: Principles of Analytic Validation of Immunohistochemical Assays: Guideline From the College of American Pathologists Pathology and Laboratory Quality Center Patrick L. Fitzgibbons (Arch Pathol Lab Med. 2014;138:1432?1443) Available free online: http://www.archivesofpathology.org/doi/pdf/10.5858/arpa.2013-0610-CP An older one, but sill applicable: Recommendations for Improved Standardization of Immunohistochemistry, Goldstein, NS, et.al., and members of Ad-Hoc Committee on Immunohistochemical Standardization, Appl Immunohistochem Mol Morph, 2007 15(2): 124-133 Book: (excellent discussion of validation and relation of validation terms to IHC) Theoretical and Practical Aspects of Test Performance, in Immunohistology: A Diagnostic Tool for the Surgical Pathologist. 3rd. Ed., Volume 19 in Major Problems in Pathology, Taylor CR and Cote RJ, Eds., W.B Saunders, Philadelphia, 2005 Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Borowicz,Wanda Sent: Tuesday, January 13, 2015 1:03 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] How are you applying this? Hi All, Below is a copy of the revised COM.40000 CAP checklist question. Now that Anatomic Pathology is having to comply with the All Common checklist, how are you applying this to your Immunohistochemistry ASR?s which are not FDA approved. We do new antibody validation and parallel testing with new lot numbers and clones. Is this enough? Can?t really see how the highlighted area pertains to this. Any advice would be appreciated. Thank. REVISED** 04/21/2014 COM.40000 Method Validation/Verification Approval Phase II There is a summary statement, signed by the laboratory director (or designee who meets CAP director qualifications) prior to use in patient testing, documenting evaluation of validation/verification studies and approval of each test for clinical use. NOTE: This checklist item is applicable only to tests implemented after June 15, 2009. The summary statement must include a written assessment of the validation/verification study, including the acceptability of the data. The summary must also include a statement approving the test for clinical use with the approval signature such as, "This validation study has been reviewed, and the performance of the method is considered acceptable for patient testing." For an FDA-cleared/approved test, a summary of the verification data must address analytic performance specifications, including analytic accuracy, precision, interferences, and reportable range, as applicable. In addition, for modified FDA-cleared/approved tests or LDTs, the summary must address analytical sensitivity, analytical specificity and any other parameter that is considered important to assure that the analytical performance of a test (e.g. specimen stability, reagent stability, linearity, carryover, and cross-contamination, etc.), as appropriate and applicable. If the laboratory makes clinical claims about its tests, the summary must address the validation of these claims. See the Method Performance Specifications section for details concerning validation/verification. Evidence of Compliance: ? Summary of validation/verification studies with review and approval REFERENCES 1) Lawrence Jennings, Vivianna M. Van Deerlin, Margaret L. Gulley (2009) Recommended Principles and Practices for Validating Clinical Molecular Pathology Tests. Archives of Pathology & Laboratory Medicine: Vol. 133, No. 5, pp. 743-755 Wanda Borowicz HT(ASCP) Histology Supervisor Sanford Health North 1720 S. University Dr. Route 1902 Fargo, ND 58103 Ph-701 417 4930 Fax-701 417 4399 wanda.borowicz@sanfordhealth.org ----------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain privileged and confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jmcgough <@t> clinlab.com Tue Jan 13 17:03:19 2015 From: jmcgough <@t> clinlab.com (=?utf-8?Q?Jason_McGough?=) Date: Tue Jan 13 16:58:03 2015 Subject: [Histonet] Histotech position opening In-Reply-To: <761E2B5697F795489C8710BCC72141FF367EA5B7@ex07.net.ucsf.edu> References: <4B322E43736F1C48B60E560707CEF2B9011701FDCE@FAREXMBX4.sanfordhealth.org> Message-ID: Clinical Laboratory of the Black Hills is a growing, independent pathology practice providing anatomic and cytologic services to Rapid City, South Dakota and surrounding communities.? Rapid City is the gateway to the Black Hills and offers a variety of four season, family friendly activities.? Our histology department processes 25,000 surgical cases, and 200 autopsies per year. We also have a progressive IHC department, and perform a variety of special stains and frozen sections. ? ? HISTOTECH Immediate opening for a certified HT/HTL (ASCP) or equivalent. Duties include embedding, microtomy, chemical and reagent management and IHC procedures. Associate Degree in related field a plus.? F/T ? Day shifts only. ? Clinical Lab offers a competitive wage with an excellent benefit t package including health, vision and dental insurance, 401(k), Profit Sharing, and disability insurance. No state income tax. Relocation assistance is available. ? Send resume to: Janet Amundson, Human Resources Clinical Laboratory of the Black Hills 2805 5th Street, Suite 210 Rapid City, South Dakota 57701 ? Fax: 605-342-0418; Phone: 605-343-2267 Email: jamundson@clinlab.com From hans <@t> histologistics.com Tue Jan 13 17:24:22 2015 From: hans <@t> histologistics.com (Hans B Snyder) Date: Tue Jan 13 17:24:29 2015 Subject: [Histonet] Vibratome services Worcester MA Message-ID: Hello All, We have a client that needs vibratome services for mouse brains. We would like this to be in the area of Worcester MA but would settle for something within 50 miles. Can anyone recommend? Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com From veronique.barres <@t> gmail.com Wed Jan 14 08:06:00 2015 From: veronique.barres <@t> gmail.com (=?UTF-8?B?VsOpcm9uaXF1ZSBCYXJyw6hz?=) Date: Wed Jan 14 08:06:49 2015 Subject: [Histonet] p16 Antibody Message-ID: Hi Histonetters! I am working in a research lab and we sometimes need to do p16 staining, but our pathologist told us that the antibody we are using right now is not good. I'd like to know which antibody you're using in your labs? Thanks for your help and happy Wednesday! V?ronique From kdwyer3322 <@t> aol.com Wed Jan 14 08:13:14 2015 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Wed Jan 14 08:13:21 2015 Subject: [Histonet] Texas Society for Histotechnology 2015 Sympsoium Convention March 20-22, 2015 Message-ID: <8D1FE1B5E87931B-15B0-69CF@webmail-vm128.sysops.aol.com> Hi Histonetters! The Texas Society for Histotechnology will be holding the 2015 S/C in Plano Texas. If you are interested in a electronic version of the program please send me a e-mail via this communication. We would love to have you join us this year! Regards, Kathy Dwyer Texas Society for Histotechnology 37th Annual Convention and Symposium Texas Histology: A Wealth ofKnowledge Dallas/Plano Marriott at Legacy TownCenter Plano, Texas March 20-22, 2015 From LSebree <@t> uwhealth.org Wed Jan 14 08:40:58 2015 From: LSebree <@t> uwhealth.org (Sebree Linda A) Date: Wed Jan 14 08:41:03 2015 Subject: [Histonet] p16 Antibody In-Reply-To: References: Message-ID: <77DD817201982748BC67D7960F2F76AF0FF121@UWHC-MBX12.uwhis.hosp.wisc.edu> We use one from BD, Veronique, with good results. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of V?ronique Barr?s Sent: Wednesday, January 14, 2015 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Antibody Hi Histonetters! I am working in a research lab and we sometimes need to do p16 staining, but our pathologist told us that the antibody we are using right now is not good. I'd like to know which antibody you're using in your labs? Thanks for your help and happy Wednesday! V?ronique _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Sarah.Dysart <@t> stdavids.com Wed Jan 14 09:01:18 2015 From: Sarah.Dysart <@t> stdavids.com (Sarah.Dysart@stdavids.com) Date: Wed Jan 14 09:01:37 2015 Subject: [Histonet] p16 Antibody In-Reply-To: <77DD817201982748BC67D7960F2F76AF0FF121@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <77DD817201982748BC67D7960F2F76AF0FF121@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: <210F59E89FE7784F9FDAD2AEC2B9E15DDAC7@FWDCWPMSGHCMD3D.hca.corpad.net> Ventana unfortunately has the market on the "good" clone...you can dilute it out pretty far (it comes pre-diluted) and still get good results however... Sarah Dysart BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor North Austin Medical Center 12221 North Mopac Expressway Austin, TX 78758 512-901-1220 (lab) 512-901-6659 (office) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, January 14, 2015 8:41 AM To: 'V?ronique Barr?s'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Antibody We use one from BD, Veronique, with good results. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of V?ronique Barr?s Sent: Wednesday, January 14, 2015 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Antibody Hi Histonetters! I am working in a research lab and we sometimes need to do p16 staining, but our pathologist told us that the antibody we are using right now is not good. I'd like to know which antibody you're using in your labs? Thanks for your help and happy Wednesday! V?ronique _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jshelley <@t> sanfordburnham.org Wed Jan 14 09:02:26 2015 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Wed Jan 14 09:02:35 2015 Subject: [Histonet] MYC-N antibody Message-ID: Good Morning, I am wondering if anyone in the clinical world is using MYC-N antibody? I would like to know who the manufacturer is and what your protocol may be. I will be running these in a research environment, first on cells and then potentially on research tissue. Your help will be greatly appreciated! Have a great day! Kind Regards! John J Shelley Research Specialist, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road Orlando, FL 32827 Tel: (407) 745-2000 Ext.2517 Lab: (407) 745-2119 Fax: (407) 745-2001 email: jshelley@sanfordburnham.org From bburnett <@t> CapeCodHealth.org Wed Jan 14 09:14:46 2015 From: bburnett <@t> CapeCodHealth.org (Burnett, Brandy) Date: Wed Jan 14 09:15:06 2015 Subject: [Histonet] p16 Antibody In-Reply-To: <210F59E89FE7784F9FDAD2AEC2B9E15DDAC7@FWDCWPMSGHCMD3D.hca.corpad.net> References: <77DD817201982748BC67D7960F2F76AF0FF121@UWHC-MBX12.uwhis.hosp.wisc.edu> <210F59E89FE7784F9FDAD2AEC2B9E15DDAC7@FWDCWPMSGHCMD3D.hca.corpad.net> Message-ID: We use the Ventana (INK4a) clone as well. What dilution do you use? Brandy Burnett Histotechnoligist, QIHC(ASCP) CCH Pathology/Histology 508-862-5267 bburnett@capecodhealth.org Expert physicians. Quality hospitals. Superior care. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah.Dysart@stdavids.com Sent: Wednesday, January 14, 2015 10:01 AM To: LSebree@uwhealth.org; veronique.barres@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Antibody Ventana unfortunately has the market on the "good" clone...you can dilute it out pretty far (it comes pre-diluted) and still get good results however... Sarah Dysart BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor North Austin Medical Center 12221 North Mopac Expressway Austin, TX 78758 512-901-1220 (lab) 512-901-6659 (office) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, January 14, 2015 8:41 AM To: 'V?ronique Barr?s'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Antibody We use one from BD, Veronique, with good results. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of V?ronique Barr?s Sent: Wednesday, January 14, 2015 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Antibody Hi Histonetters! I am working in a research lab and we sometimes need to do p16 staining, but our pathologist told us that the antibody we are using right now is not good. I'd like to know which antibody you're using in your labs? Thanks for your help and happy Wednesday! V?ronique _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From mward <@t> wakehealth.edu Wed Jan 14 09:21:22 2015 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Wed Jan 14 09:21:35 2015 Subject: [Histonet] p16 Antibody In-Reply-To: References: <77DD817201982748BC67D7960F2F76AF0FF121@UWHC-MBX12.uwhis.hosp.wisc.edu> <210F59E89FE7784F9FDAD2AEC2B9E15DDAC7@FWDCWPMSGHCMD3D.hca.corpad.net> Message-ID: We dilute ours 1:5 from the predilute and run it on the Bond 3. It works very well. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Wednesday, January 14, 2015 10:15 AM To: 'Sarah.Dysart@stdavids.com'; LSebree@uwhealth.org; veronique.barres@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Antibody We use the Ventana (INK4a) clone as well. What dilution do you use? Brandy Burnett Histotechnoligist, QIHC(ASCP) CCH Pathology/Histology 508-862-5267 bburnett@capecodhealth.org Expert physicians. Quality hospitals. Superior care. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sarah.Dysart@stdavids.com Sent: Wednesday, January 14, 2015 10:01 AM To: LSebree@uwhealth.org; veronique.barres@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Antibody Ventana unfortunately has the market on the "good" clone...you can dilute it out pretty far (it comes pre-diluted) and still get good results however... Sarah Dysart BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor North Austin Medical Center 12221 North Mopac Expressway Austin, TX 78758 512-901-1220 (lab) 512-901-6659 (office) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, January 14, 2015 8:41 AM To: 'V?ronique Barr?s'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Antibody We use one from BD, Veronique, with good results. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of V?ronique Barr?s Sent: Wednesday, January 14, 2015 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Antibody Hi Histonetters! I am working in a research lab and we sometimes need to do p16 staining, but our pathologist told us that the antibody we are using right now is not good. I'd like to know which antibody you're using in your labs? Thanks for your help and happy Wednesday! V?ronique _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. Helpdesk@CapeCodHealth.org From Robin.Ryan <@t> hcahealthcare.com Wed Jan 14 09:39:50 2015 From: Robin.Ryan <@t> hcahealthcare.com (Robin.Ryan@hcahealthcare.com) Date: Wed Jan 14 09:39:57 2015 Subject: [Histonet] breast tissue and radioactive seeds Message-ID: <2ACE5E628245274AAC3A1BA4AB2F926CD8DB@FWDCWPMSGHCMD3B.hca.corpad.net> Hi Everyone, After 5 years of being in the private sector I am now back in the wonderful environment of a hospital setting as the Histology supervisor. We are going to be starting a new procedure here called "Conversion to Radioactive Seed Localized Breast Surgery" and I have been asked to come to this meeting. As I have been strictly in Dermatology for the past few years I am not familiar with this process. I have dealt with the prostate radioactive seeds before but not sure if this will involve the Histology lab in the form of surgical tissue or not. I would like to be as informed as possible before the meeting which is next week, so I came to the source I value the most. Can anyone share any information with me? Thanks so much, Kaye From becky.garrison <@t> jax.ufl.edu Wed Jan 14 10:22:25 2015 From: becky.garrison <@t> jax.ufl.edu (Garrison, Becky) Date: Wed Jan 14 10:22:34 2015 Subject: [Histonet] RE: breast tissue and radioactive seeds In-Reply-To: <2ACE5E628245274AAC3A1BA4AB2F926CD8DB@FWDCWPMSGHCMD3B.hca.corpad.net> References: <2ACE5E628245274AAC3A1BA4AB2F926CD8DB@FWDCWPMSGHCMD3B.hca.corpad.net> Message-ID: <9E47DE9D490DCC42A2EAE94F22BF93F2DB8505BE@JX1B-MAIL1.umc.ufl.edu> If this is the I-125 seed localization procedure for breast casses: our Radiology dept here does this and I can tell you what we do here. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robin.Ryan@hcahealthcare.com Sent: Wednesday, January 14, 2015 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] breast tissue and radioactive seeds Hi Everyone, After 5 years of being in the private sector I am now back in the wonderful environment of a hospital setting as the Histology supervisor. We are going to be starting a new procedure here called "Conversion to Radioactive Seed Localized Breast Surgery" and I have been asked to come to this meeting. As I have been strictly in Dermatology for the past few years I am not familiar with this process. I have dealt with the prostate radioactive seeds before but not sure if this will involve the Histology lab in the form of surgical tissue or not. I would like to be as informed as possible before the meeting which is next week, so I came to the source I value the most. Can anyone share any information with me? Thanks so much, Kaye _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JRobinson <@t> pathology-associates.com Wed Jan 14 10:25:14 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Wed Jan 14 10:25:32 2015 Subject: [Histonet] Tissue exchange- Coccidiodes for fungus Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D899AD@PAEXCH1.PathologyAssociates.local> I checked with NSH and they say that their tissue exchange bank has been discontinued. I have a lot of lung tissue containing Coccidiodes organisms (Valley Fever). I would like to trade for some tissue containing fungus. Is there another tissue exchange network out there? Is anyone interested in trading? Hi Tim. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From vrivera <@t> westderm.com Wed Jan 14 10:36:05 2015 From: vrivera <@t> westderm.com (Vincent Rivera) Date: Wed Jan 14 10:36:14 2015 Subject: [Histonet] RE: Tissue exchange- Coccidiodes for fungus In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D899AD@PAEXCH1.PathologyAssociates.local> References: <204A03EB5A7F0A4BB1EEDD52A963829C16D899AD@PAEXCH1.PathologyAssociates.local> Message-ID: <3D4A471B82E7A44C87F6839732320D9F014047CE0F@VSPDMS-ITEXMB02.DMS.COM> Hi Jeff, The Michigan Society of Histotechnologist has a tissue exchange bank available to all MSH members, so you might want to check with them. Regards, Vincent Rivera, HT (ASCP) QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vrivera@westderm.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Wednesday, January 14, 2015 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue exchange- Coccidiodes for fungus I checked with NSH and they say that their tissue exchange bank has been discontinued. I have a lot of lung tissue containing Coccidiodes organisms (Valley Fever). I would like to trade for some tissue containing fungus. Is there another tissue exchange network out there? Is anyone interested in trading? Hi Tim. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike <@t> pathview.com Wed Jan 14 10:49:09 2015 From: mike <@t> pathview.com (Michael Mihalik) Date: Wed Jan 14 10:49:20 2015 Subject: [Histonet] Cassette lableler and slide labeler In-Reply-To: <9B1A1501A800064397369BD8072E6BCA96B378@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA96B378@E2K10DB.springfieldclinic.com> Message-ID: <00d301d0301a$04d698c0$0e83ca40$@pathview.com> James, I think I can respond to this as I am an LIS vendor. The short answer is that it should NOT be an issue, ASSUMING your LIS vendor can communicate/interface with the two instruments. Once the barcode is on the cassette or the slide, it becomes a standalone entity. The barcode on the cassette or slide then gets read by a barcode scanner. Every scanner I've run across can read multiple symbologies (bar code types, if you will). Some scanners read better than others, though. I am assuming that you're generating cassettes and slides through your LIS. If you're using the cassette/slide printer vendor's software to do this, then that changes things. If you'd like further clarification or discussion, feel free to email me. Michael Mihalik PathView Systems |?cell: 214.733.7688?| 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Tuesday, January 13, 2015 7:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette lableler and slide labeler I didn't plan on this however wondered if anyone had a General Data system for making cassettes but a different setup for making slides, such as a Thermo Slidemate. I suspect there are issues with the LIS system, barcoding, etc. Am I wrong? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Wed Jan 14 10:59:50 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Wed Jan 14 11:00:00 2015 Subject: [Histonet] RE: Cassette lableler and slide labeler In-Reply-To: <9B1A1501A800064397369BD8072E6BCA96B378@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA96B378@E2K10DB.springfieldclinic.com> Message-ID: <761E2B5697F795489C8710BCC72141FF367EA94C@ex07.net.ucsf.edu> Jim, by "system" do you mean using General Data software/printer to produce the barcodes, outside of your LIS, or just using the cassette printer with your LIS? If you are using a third party system to track materials outside of the LIS, then everything downstream has to use that system as well, assuming the LIS does produce the codes, so cannot recognize them. In that case all tracking is in a system outside the LIS. However, using a different company's slide printer with the third party system will not be an issue if there is a print driver for the third party software to drive that printer. If they have to develop a print driver, that could cost you some $$$$. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Tuesday, January 13, 2015 7:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette lableler and slide labeler I didn't plan on this however wondered if anyone had a General Data system for making cassettes but a different setup for making slides, such as a Thermo Slidemate. I suspect there are issues with the LIS system, barcoding, etc. Am I wrong? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HernandezJW <@t> uthscsa.edu Wed Jan 14 13:59:47 2015 From: HernandezJW <@t> uthscsa.edu (Hernandez, Jesus Willibaldo) Date: Wed Jan 14 13:59:53 2015 Subject: [Histonet] MMA Troubleshooting for Undecalcified Bone Message-ID: This is intended for Bone Histology samples embedded in MMA. I had two main questions. 1. After mounting my tissue sections (5-10microns) with Haupts adhesive, I noticed that the bone within the section was appearing opaque/darkened on the microscope? I will add that the morphology of the bone structure is not present. The bone still stains, but the detail is missing. I am thinking that I might of over dehydrated during processing or my microtome blade is dull. Any suggestions about this problem would be appreciated. 2. Anyone have a Hematoxylin & Eosin Staining protocol for undecalcified MMA embedded bone? I do have a protocol, but not sure if I was missing a couple of steps for proper staining. Thank you all. Regards, Jesse Hernandez From Yves.Heremans <@t> vub.ac.be Wed Jan 14 16:03:23 2015 From: Yves.Heremans <@t> vub.ac.be (Yves Heremans) Date: Wed Jan 14 16:03:34 2015 Subject: [Histonet] marking small samples Message-ID: Dear Histonetters, Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? Preferably something that would not interfere with subsequent antibody staining. Yves From jmcgough <@t> clinlab.com Wed Jan 14 16:21:44 2015 From: jmcgough <@t> clinlab.com (=?utf-8?Q?Jason_McGough?=) Date: Wed Jan 14 16:16:25 2015 Subject: [Histonet] marking small samples In-Reply-To: References: Message-ID: Eosin in the alcohol works great to mark these small pieces of tissue. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgough@clinlab.com www.clinlab.com -----Original message----- > From:Yves Heremans > > Sent: Wednesday, January 14, 2015 3:11 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] marking small samples > > Dear Histonetters, > > Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? > Preferably something that would not interfere with subsequent antibody staining. > > Yves > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From mw <@t> personifysearch.com Wed Jan 14 16:20:16 2015 From: mw <@t> personifysearch.com (Matt Ward) Date: Wed Jan 14 16:20:22 2015 Subject: [Histonet] New Histology Opportunities - CA Message-ID: <06d701d03048$46e02e60$d4a08b20$@personifysearch.com> Hello Histonet! We hope everyone is having a great 2015. One of our leading clients has opened a few Field Based Histology Support opportunities based on the west coast. The ideal candidate will have an IHC background and interested in a customer facing support/specialist type opportunity. The position offers a very competitive package including a salary, bonus, company car, cell phone, laptop, full benefits. If this sounds like something you may be interested in learning more about, please contact me directly at mw@personifysearch.com . Thanks! Matt Matt Ward Program Manager Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 919.459.3654 (Tel) 800.875.6188 direct ext 103 (Fax) 919.882.8727 www.personifysearch.com From Timothy.Morken <@t> ucsf.edu Wed Jan 14 16:30:12 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Wed Jan 14 16:30:28 2015 Subject: [Histonet] marking small samples In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF367EAD18@ex07.net.ucsf.edu> We use eosin the processor alcohol. Also we use blue-tinted paraffin which helps with contrast on small bx Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yves Heremans Sent: Wednesday, January 14, 2015 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking small samples Dear Histonetters, Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? Preferably something that would not interfere with subsequent antibody staining. Yves _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alineumann <@t> aol.com Wed Jan 14 16:45:48 2015 From: alineumann <@t> aol.com (Alice Neumann) Date: Wed Jan 14 16:45:52 2015 Subject: [Histonet] Message to renew subscription. Message-ID: <8D1FE62F98B19F2-21E4-9955@webmail-va036.sysops.aol.com> Got a message to renew subscription or it will be cut off.? Would like to continue receiving histonet.? Is this message from some criminal or from histonet?? Alice Neumann MD Pinnacle Pathology PC 207 Auburn Dr. ?#1 Auburn, AL 36830 24 Hour Cell Phone:? 307-413-4092 alineumann@aol.com ? From mburns <@t> atlanticurologyclinics.com Wed Jan 14 16:56:51 2015 From: mburns <@t> atlanticurologyclinics.com (Melissa Burns) Date: Wed Jan 14 16:57:03 2015 Subject: [Histonet] marking small samples In-Reply-To: <761E2B5697F795489C8710BCC72141FF367EAD18@ex07.net.ucsf.edu> References: , <761E2B5697F795489C8710BCC72141FF367EAD18@ex07.net.ucsf.edu> Message-ID: I've been told Eosin affects a lot of molecular testing. We used to use it in our process but discontinued to not interfere with future testing. Sent from my iPhone > On Jan 14, 2015, at 5:31 PM, "Morken, Timothy" wrote: > > We use eosin the processor alcohol. Also we use blue-tinted paraffin which helps with contrast on small bx > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yves Heremans > Sent: Wednesday, January 14, 2015 2:03 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] marking small samples > > Dear Histonetters, > > Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? > Preferably something that would not interfere with subsequent antibody staining. > > Yves > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegghm <@t> hotmail.com Wed Jan 14 16:59:35 2015 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Wed Jan 14 16:59:47 2015 Subject: [Histonet] marking small samples In-Reply-To: References: , , <761E2B5697F795489C8710BCC72141FF367EAD18@ex07.net.ucsf.edu>, Message-ID: Eosin can be a problem in IHC as well especially if you use alk phos red detection. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com > From: mburns@atlanticurologyclinics.com > To: Timothy.Morken@ucsf.edu > Date: Wed, 14 Jan 2015 22:56:51 +0000 > Subject: Re: [Histonet] marking small samples > CC: histonet@lists.utsouthwestern.edu; Yves.Heremans@vub.ac.be > > I've been told Eosin affects a lot of molecular testing. We used to use it in our process but discontinued to not interfere with future testing. > > Sent from my iPhone > > > On Jan 14, 2015, at 5:31 PM, "Morken, Timothy" wrote: > > > > We use eosin the processor alcohol. Also we use blue-tinted paraffin which helps with contrast on small bx > > > > Tim Morken > > Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies > > UC San Francisco Medical Center > > San Francisco, CA > > > > CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yves Heremans > > Sent: Wednesday, January 14, 2015 2:03 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] marking small samples > > > > Dear Histonetters, > > > > Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? > > Preferably something that would not interfere with subsequent antibody staining. > > > > Yves > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Wed Jan 14 17:28:49 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Wed Jan 14 17:29:11 2015 Subject: [Histonet] marking small samples In-Reply-To: References: , <761E2B5697F795489C8710BCC72141FF367EAD18@ex07.net.ucsf.edu> Message-ID: <761E2B5697F795489C8710BCC72141FF367EADA7@ex07.net.ucsf.edu> Last year I found a paper to the effect that eosin may interfere with PCR analysis. Our molecular group did an internal study and did not find any significant issues. But I just found another paper stating no interference: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3383644/ Am J Clin Pathol. Jul 2012; 138(1): 122-129. No Evidence for Interference of Hematoxylin and Eosin (HE) Staining in DNA Testing: Utility of DNA Extraction from HE-Stained Archival Tissue Sections Teppei Morikawa, MD im Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: Melissa Burns [mailto:mburns@atlanticurologyclinics.com] Sent: Wednesday, January 14, 2015 2:57 PM To: Morken, Timothy Cc: Yves Heremans; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] marking small samples I've been told Eosin affects a lot of molecular testing. We used to use it in our process but discontinued to not interfere with future testing. Sent from my iPhone > On Jan 14, 2015, at 5:31 PM, "Morken, Timothy" wrote: > > We use eosin the processor alcohol. Also we use blue-tinted paraffin > which helps with contrast on small bx > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special > Studies UC San Francisco Medical Center San Francisco, CA > > CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yves > Heremans > Sent: Wednesday, January 14, 2015 2:03 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] marking small samples > > Dear Histonetters, > > Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? > Preferably something that would not interfere with subsequent antibody staining. > > Yves > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegghm <@t> hotmail.com Wed Jan 14 17:31:23 2015 From: pruegghm <@t> hotmail.com (Patsy Ruegg) Date: Wed Jan 14 17:31:39 2015 Subject: [Histonet] marking small samples In-Reply-To: <761E2B5697F795489C8710BCC72141FF367EADA7@ex07.net.ucsf.edu> References: , , <761E2B5697F795489C8710BCC72141FF367EAD18@ex07.net.ucsf.edu>, , <761E2B5697F795489C8710BCC72141FF367EADA7@ex07.net.ucsf.edu> Message-ID: Thanks for your research Tim, this is good to know. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm@hotmail.com > From: Timothy.Morken@ucsf.edu > To: mburns@atlanticurologyclinics.com > Date: Wed, 14 Jan 2015 23:28:49 +0000 > Subject: RE: [Histonet] marking small samples > CC: histonet@lists.utsouthwestern.edu > > Last year I found a paper to the effect that eosin may interfere with PCR analysis. Our molecular group did an internal study and did not find any significant issues. > > But I just found another paper stating no interference: > > http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3383644/ > > Am J Clin Pathol. Jul 2012; 138(1): 122-129. > No Evidence for Interference of Hematoxylin and Eosin (HE) Staining in DNA Testing: Utility of DNA Extraction from HE-Stained Archival Tissue Sections > Teppei Morikawa, MD > > > > im Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies > UC San Francisco Medical Center > San Francisco, CA > > CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. > > > -----Original Message----- > From: Melissa Burns [mailto:mburns@atlanticurologyclinics.com] > Sent: Wednesday, January 14, 2015 2:57 PM > To: Morken, Timothy > Cc: Yves Heremans; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] marking small samples > > I've been told Eosin affects a lot of molecular testing. We used to use it in our process but discontinued to not interfere with future testing. > > Sent from my iPhone > > > On Jan 14, 2015, at 5:31 PM, "Morken, Timothy" wrote: > > > > We use eosin the processor alcohol. Also we use blue-tinted paraffin > > which helps with contrast on small bx > > > > Tim Morken > > Supervisor, Histology, Electron Microscopy and Neuromuscular Special > > Studies UC San Francisco Medical Center San Francisco, CA > > > > CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yves > > Heremans > > Sent: Wednesday, January 14, 2015 2:03 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] marking small samples > > > > Dear Histonetters, > > > > Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? > > Preferably something that would not interfere with subsequent antibody staining. > > > > Yves > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From blayjorge <@t> gmail.com Wed Jan 14 18:31:02 2015 From: blayjorge <@t> gmail.com (Jorge A. Santiago-Blay) Date: Wed Jan 14 18:31:07 2015 Subject: [Histonet] Of possible interest to some Histonet Listers Message-ID: Dear Histonet Listers: The following link, http://blaypublishers.com/category/previous-issues/ , contains the hitherto published issues of a peer-reviewed, scientific journal I run, called* Life: The Excitement of Biology*. Some of the articles deal with subjects of possible interest to some of you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html From Philip.Gibson <@t> nuth.nhs.uk Thu Jan 15 04:10:32 2015 From: Philip.Gibson <@t> nuth.nhs.uk (Gibson, Philip) Date: Thu Jan 15 04:10:54 2015 Subject: [Histonet] RE: breast tissue and radioactive seeds Message-ID: <20150115101036.DC4364495F6@nhs-pd1e-esg108.ad1.nhs.net> Hi Kaye We've recently started using this procedure, so a (very) brief synopsis as follows: Rather than a wire localisation of a breast tumour (using ultrasound) where a surgeon follows the wire to excise the tumour, a tiny radioactive seed is used instead. The seed is injected by syringe into the tumour, where it is "held" by the tissue (rather than a wire which can become dislodged by movement of the patient). The surgeon then uses a fine-tuned radiation meter to locate the seed (and thus the tumour) for excision. >From theatre, the breast excision specimen is x-rayed by our Radiology Dept to confirm that tumour does not appear to be at the resection margins (if it does, then a re-excision is done). Critically, because the seeds can be placed totally within the tumour, very few immediate shave re-excisions are required - this is the great advantage. The histology lab receives the specimen, where we need to be very careful not to fracture/cut the seed. We use our own x-ray cabinet to show whereabouts in the specimen the seed is lying. Once the specimen is sliced, the seed is retrieved and the specimen trimmed as normal. Here in the UK, the main concern over this technique is that no source of radiation can be lost (down a trimming bench sink, onto the floor etc.). We therefore have a complex chain of custody record which confirms the presence of the seed by radiation monitor at all areas of the hospital. Hope this helps! Phil Date: Wed, 14 Jan 2015 15:39:50 +0000 From: > Subject: [Histonet] breast tissue and radioactive seeds To: > Message-ID: <2ACE5E628245274AAC3A1BA4AB2F926CD8DB@FWDCWPMSGHCMD3B.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" Hi Everyone, After 5 years of being in the private sector I am now back in the wonderful environment of a hospital setting as the Histology supervisor. We are going to be starting a new procedure here called "Conversion to Radioactive Seed Localized Breast Surgery" and I have been asked to come to this meeting. As I have been strictly in Dermatology for the past few years I am not familiar with this process. I have dealt with the prostate radioactive seeds before but not sure if this will involve the Histology lab in the form of surgical tissue or not. I would like to be as informed as possible before the meeting which is next week, so I came to the source I value the most. Can anyone share any information with me? Thanks so much, Kaye ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ******************************************************************************************************************** From amurvosh <@t> advancederm.net Thu Jan 15 07:19:58 2015 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Thu Jan 15 07:20:05 2015 Subject: [Histonet] marking small samples In-Reply-To: References: Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7A60EB7@Exchange.Advancederm.net> I mark shaves and punches with .5% Toludine blue it won't color the skin but will color the rest. And best of all it doesn't show up in any staining its purely for identifying your tissue for embedding purposes. Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yves Heremans Sent: Wednesday, January 14, 2015 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking small samples Dear Histonetters, Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? Preferably something that would not interfere with subsequent antibody staining. Yves _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HernandezJW <@t> uthscsa.edu Thu Jan 15 10:31:35 2015 From: HernandezJW <@t> uthscsa.edu (Hernandez, Jesus Willibaldo) Date: Thu Jan 15 10:31:45 2015 Subject: [Histonet] MMA Troubleshooting for Undecalcified Bone Message-ID: This is intended for Bone Histology samples embedded in MMA. I had two main questions. 1. After mounting my tissue sections (5-10microns) with Haupts adhesive, I noticed that the bone within the section was appearing opaque/darkened on the microscope? I will add that the morphology of the bone structure is not present. The bone still stains, but the detail is missing. I am thinking that I might of over dehydrated during processing or my microtome blade is dull. Any suggestions about this problem would be appreciated. 2. Anyone have a Hematoxylin & Eosin Staining protocol for undecalcified MMA embedded bone? I do have a protocol, but not sure if I was missing a couple of steps for proper staining. Thank you all. Regards, Jesse Hernandez From hans <@t> histologistics.com Thu Jan 15 11:45:19 2015 From: hans <@t> histologistics.com (Hans B Snyder) Date: Thu Jan 15 11:45:32 2015 Subject: [Histonet] Vibratome Worcester MA Message-ID: Hello All, We have a client that needs vibratome services for mouse brains. We would like this to be in the area of Worcester MA but would settle for something within 50 miles. Can anyone recommend? Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com From rmweber113 <@t> comcast.net Thu Jan 15 12:16:24 2015 From: rmweber113 <@t> comcast.net (rmweber113@comcast.net) Date: Thu Jan 15 12:16:43 2015 Subject: [Histonet] Cytology Workload In-Reply-To: <472301067.2017147.1421345487158.JavaMail.zimbra@comcast.net> Message-ID: <1410065432.2022238.1421345784358.JavaMail.zimbra@comcast.net> Hi,? I have a question about cytology workload.? If you have a cytology tech that works at another facility reading slides and then comes to your lab at night to read slides, do they have to provide you with a log that they keep at their day job as to what they read during that day?? In other words do I have to get in writing what they read that day?at their other job ?or do I just take their word for it when they fill out my workload log. And is it illegal to ask for such a log from them? ? Thank you, Marilynn Weber H.T.(ASCP)QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 From rjbuesa <@t> yahoo.com Thu Jan 15 12:39:57 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jan 15 12:40:08 2015 Subject: [Histonet] Cytology Workload In-Reply-To: <1410065432.2022238.1421345784358.JavaMail.zimbra@comcast.net> References: <472301067.2017147.1421345487158.JavaMail.zimbra@comcast.net> <1410065432.2022238.1421345784358.JavaMail.zimbra@comcast.net> Message-ID: <838848653.1059715.1421347197674.JavaMail.yahoo@jws100136.mail.ne1.yahoo.com> They should provide you with the cytotech work load.You HAVE? to know it!It is not illegal and the cytotech if s/he wants to work for your lab is REQUIRED to help you obtain that information.The workload limit/8 hours includes ALL work in ALL places.Ren? J.? On Thursday, January 15, 2015 1:16 PM, "rmweber113@comcast.net" wrote: Hi,? I have a question about cytology workload.? If you have a cytology tech that works at another facility reading slides and then comes to your lab at night to read slides, do they have to provide you with a log that they keep at their day job as to what they read during that day?? In other words do I have to get in writing what they read that day?at their other job ?or do I just take their word for it when they fill out my workload log. And is it illegal to ask for such a log from them? ? Thank you, Marilynn Weber H.T.(ASCP)QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From doug.porter <@t> caplab.org Thu Jan 15 13:11:58 2015 From: doug.porter <@t> caplab.org (Douglas Porter) Date: Thu Jan 15 13:12:29 2015 Subject: [Histonet] Cytology Workload In-Reply-To: <1410065432.2022238.1421345784358.JavaMail.zimbra@comcast.net> References: <472301067.2017147.1421345487158.JavaMail.zimbra@comcast.net> <1410065432.2022238.1421345784358.JavaMail.zimbra@comcast.net> Message-ID: <002101d030f7$231c7fb0$69557f10$@caplab.org> CLIA and CAP require that Cytotechs only read 100 Paps per day. It is the responsibility of the second workplace to ensure that the Cytotech doesn't cross the 100 Pap barrier. Because you are required to ensure compliance, they should report to you how many Paps they screened before they arrive at your lab. How you arrive at that number is up to you. They may not what to share their workload for obvious reasons, but that's too bad. If they want the work, they should provide you with something that you can use in the event your CAP or other inspector asks for the proof. Get the documentation!! Douglas A. Porter, HT (ASCP) Pathologist Assistant IT Coordinator Sparrow / CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.porter@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rmweber113@comcast.net Sent: Thursday, January 15, 2015 1:16 PM To: histonet Subject: [Histonet] Cytology Workload Hi, I have a question about cytology workload. If you have a cytology tech that works at another facility reading slides and then comes to your lab at night to read slides, do they have to provide you with a log that they keep at their day job as to what they read during that day? In other words do I have to get in writing what they read that dayat their other job or do I just take their word for it when they fill out my workload log. And is it illegal to ask for such a log from them? Thank you, Marilynn Weber H.T.(ASCP)QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----- No virus found in this message. Checked by AVG - www.avg.com Version: 2015.0.5645 / Virus Database: 4260/8932 - Release Date: 01/14/15 From joelleweaver <@t> hotmail.com Thu Jan 15 13:51:15 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Thu Jan 15 13:51:25 2015 Subject: [Histonet] How are you applying this? In-Reply-To: <4B322E43736F1C48B60E560707CEF2B9011701FDCE@FAREXMBX4.sanfordhealth.org> References: <4B322E43736F1C48B60E560707CEF2B9011701FDCE@FAREXMBX4.sanfordhealth.org> Message-ID: Yes, have prepared this summary for each newly validated AB over the past couple of years, with the included statement and signature of the medical director. Joelle Weaver MAOM, HTL (ASCP) QIHC From: Wanda.Borowicz@sanfordhealth.org To: histonet@lists.utsouthwestern.edu Date: Tue, 13 Jan 2015 21:02:53 +0000 Subject: [Histonet] How are you applying this? Hi All, Below is a copy of the revised COM.40000 CAP checklist question. Now that Anatomic Pathology is having to comply with the All Common checklist, how are you applying this to your Immunohistochemistry ASR?s which are not FDA approved. We do new antibody validation and parallel testing with new lot numbers and clones. Is this enough? Can?t really see how the highlighted area pertains to this. Any advice would be appreciated. Thank. REVISED** 04/21/2014 COM.40000 Method Validation/Verification Approval Phase II There is a summary statement, signed by the laboratory director (or designee who meets CAP director qualifications) prior to use in patient testing, documenting evaluation of validation/verification studies and approval of each test for clinical use. NOTE: This checklist item is applicable only to tests implemented after June 15, 2009. The summary statement must include a written assessment of the validation/verification study, including the acceptability of the data. The summary must also include a statement approving the test for clinical use with the approval signature such as, "This validation study has been reviewed, and the performance of the method is considered acceptable for patient testing." For an FDA-cleared/approved test, a summary of the verification data must address analytic performance specifications, including analytic accuracy, precision, interferences, and reportable range, as applicable. In addition, for modified FDA-cleared/approved tests or LDTs, the summary must address analytical sensitivity, analytical specificity and any other parameter that is considered important to assure that the analytical performance of a test (e.g. specimen stability, reagent stability, linearity, carryover, and cross-contamination, etc.), as appropriate and applicable. If the laboratory makes clinical claims about its tests, the summary must address the validation of these claims. See the Method Performance Specifications section for details concerning validation/verification. Evidence of Compliance: ? Summary of validation/verification studies with review and approval REFERENCES 1) Lawrence Jennings, Vivianna M. Van Deerlin, Margaret L. Gulley (2009) Recommended Principles and Practices for Validating Clinical Molecular Pathology Tests. Archives of Pathology & Laboratory Medicine: Vol. 133, No. 5, pp. 743-755 Wanda Borowicz HT(ASCP) Histology Supervisor Sanford Health North 1720 S. University Dr. Route 1902 Fargo, ND 58103 Ph-701 417 4930 Fax-701 417 4399 wanda.borowicz@sanfordhealth.org ----------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain privileged and confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Thu Jan 15 15:46:27 2015 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Thu Jan 15 15:46:33 2015 Subject: [Histonet] Heme "boogers" Message-ID: Hello All! We have been having an interesting problem with our Hematoxylin. It seems there are globules that form on the bottom of our hematoxylin wells in our automatic stainer (Leica XL). These globs are acellular, so it is not a fungus or something. We use a progressive Mayer's Hematoxylin formula as follows: 1000ml DI 50g Aluminum Ammonium Sulfate 1g Hematoxylin powder 1g Citric Acid 0.2g Sodium Iodate Anyone know what this might be? It doesn't affect the staining, but likes to hang on the bottom of the staining racks and is a bit gross. Plus it gets dragged into some of the following wells on occasion. Thanks! Claire From hans <@t> histologistics.com Thu Jan 15 15:51:58 2015 From: hans <@t> histologistics.com (Hans B Snyder) Date: Thu Jan 15 15:52:13 2015 Subject: [Histonet] Heme "boogers" In-Reply-To: References: Message-ID: <5F5134C7-C76A-41A6-9D9D-F8C277A36C9F@histologistics.com> That's the aluminum ammonium precipitating out, it happens with Harris also. Do you filter it before use? Histologistics Hans B Snyder 508.308.7800 Hans@histologistics.com > On Jan 15, 2015, at 16:46, Ingles Claire wrote: > > Hello All! > > > > We have been having an interesting problem with our Hematoxylin. It seems there are globules that form on the bottom of our hematoxylin wells in our automatic stainer (Leica XL). These globs are acellular, so it is not a fungus or something. We use a progressive Mayer's Hematoxylin formula as follows: > > > > 1000ml DI > > 50g Aluminum Ammonium Sulfate > > 1g Hematoxylin powder > > 1g Citric Acid > > 0.2g Sodium Iodate > > > > Anyone know what this might be? It doesn't affect the staining, but likes to hang on the bottom of the staining racks and is a bit gross. Plus it gets dragged into some of the following wells on occasion. > > > > Thanks! > > Claire > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CThornton <@t> dahlchase.com Thu Jan 15 15:50:47 2015 From: CThornton <@t> dahlchase.com (Clare Thornton) Date: Thu Jan 15 15:53:05 2015 Subject: [Histonet] Epitomics Message-ID: Does anyone know if there is an Epitomics representative for the northeast (New England)? I've emailed the company but have not heard anything back. Thank you! Clare Clare J. Thornton, HTL(ASCP),QIHC Lead Immunohistochemistry Tech Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton@dahlchase.com From tgenade <@t> gmail.com Thu Jan 15 17:56:51 2015 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Thu Jan 15 17:56:58 2015 Subject: [Histonet] preferable thickness of sections for diagnostic demonstration of neurodegenerative pathologies Message-ID: Hello, I am planning a study and need advice on the optimum tissue thickness for diagnosis of: alpha-synucleinopathies tauopathies amyloid plaques The study is primarily comparative distribution and frequency between age groups and would be looking to compare "affected neurons per field of vision/total neurons visible" rather than stereological cell counts (which are beyond my budget at this time). I plan on using fixed paraffin embedded tissue. The GE site ( http://www.clarientinc.com/test-menu.aspx?AlphabeticalFilter=A&TestID=2571 ) suggest 4 micron for Lewy Bodies. Any particular reason? Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From Timothy.Morken <@t> ucsf.edu Thu Jan 15 18:18:16 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Thu Jan 15 18:18:28 2015 Subject: [Histonet] Histology Supervisor positon, UCSF Message-ID: <761E2B5697F795489C8710BCC72141FF367ECF5A@ex07.net.ucsf.edu> We are still taking applications for the position of Supervisor, Histology and IHC at UC San Francisco Medical Center. See job description below this message. The ideal candidate will have extensive experience in histology, immunohistochemistry and supervising a large laboratory as well as extensive experience interacting one on one with pathologists, residents, administration and vendors. We have 14 histotechs and state of the art equipment. We implemented full department barcoding 9 months ago. We have 41 pathologists and 49 residents/fellows. We will open a new hospital complex in two weeks which will give us three major facilities across the city. All feed into the histology department If interested contact me and I will send more details on our operations. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. Search "histology" at this webpage to find the position and apply https://careers.ucsfmedicalcenter.org:8443/psp/hcmprd_cg/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_CE.GBL Job ID: 5546 Job Title: HISTOLOGY AND IPOX SUPERVISOR Job Code: 7902 Department: Pathology-Surgical / Histology Location: Parnassus Full/Part Time: Full-Time Appointment Type: Career Shift: Variable Variable Weekly Hours: 40 ; 100% Union Information: This classification is not represented by a union Our legacy of unsurpassed patient care and unceasing mission to integrate high-tech medical research with clinical operations has led to our prestigious standing as one of the top 10 hospitals in the nation according to U.S. News & World Report. As a premier health care institution dedicated to advancing health worldwide, UCSF Medical Center can also be the best place to advance and shape your career. The medical center's employees are one of the most important reasons why we are recognized as one of the nation's best hospitals. To work at UCSF Medical Center is to be part of an institution that provides the highest caliber of care to patients; a nurturing, dynamic and team-oriented atmosphere in which to best use your skills and talents. Job Summary Under general direction from the Medical Director, the incumbent serves as supervisor to 3 Lead Histotechnologists and 9 Histotechnologists. In addition to supervisory duties, the incumbent serves as a technical expert providing direction to staff as well as performing all technical aspects of surgical histology and immunohistochemistry procedures. As supervisor: recruits, hires, trains, completes performance evaluations, and resolves employee issues. Provides orientation, completes competency assessments, maintains staff schedules, training and compliance documentation. Implements new tests and procedures as requested by Medical Directors. Updates and maintains the lab manuals and annual reviews required for accreditation. Integrates new technologies into the laboratory to include analyzing and selecting of new equipment that meets space constraints and operational needs. Ensures that new equipment and associated validation and staff training are performed. Ensures that all equipment is well-maintained. Ensures that staff training and documentation are completed as required. Ensures that QC documentation is complete and quality standards are maintained in the laboratory. Advises and assists researchers planning research projects and determines the ability of the laboratory to accommodate research projects, updating the Medical Director as required. Other duties as assigned. Sign-on Bonus and/or Relocation is available for this position! Required Qualifications * College degree in a biological science, chemistry or a related field or equivalent education, Work experience experience as a histotechnologist in a comparable high-volume hospital histology laboratory within the last seven years, including senior-level experience. * Demonstrated high-volume, high-quality sectioning and staining skills. * Ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines. * Excellent interpersonal and communication skills. * ASCP certification: HT licensed or eligible required (candidate will be expected to obtain certification within 1 year), HTL desirable. * Demonstrated ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines required. * Previous, recent supervisory experience in a Histology and /or Immunoperoxidase Laboratory required. * Must be experienced with information technology systems and the use of hardware and software in business and laboratory settings. * Must be an effective user of spreadsheets and database software. Preferred Qualifications * ASCP certification * HT or HTL-licensed strongly preferred. * Previous leadership experience in a hospital laboratory. Licensure/Certification N/A Equal Employment Opportunity UCSF is an Equal Opportunity/Affirmative Action Employer. All qualified applicants are encouraged to apply. Further information about the University of California, San Francisco, is available at diversity.ucsf.edu. UCSF seeks candidates whose skills, and personal and professional experience, have prepared them to contribute to our commitment to diversity and excellence, and the communities we serve. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.morken@ucsfmedctr.org CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. From naje1972 <@t> yahoo.com Thu Jan 15 18:19:36 2015 From: naje1972 <@t> yahoo.com (naje1972) Date: Thu Jan 15 18:19:49 2015 Subject: =?US-ASCII?Q?RE:_[Histonet]_preferable_?= =?US-ASCII?Q?thickness_of_sections_for_di?= =?US-ASCII?Q?agnostic=0D__demonstration_of_neurodegenerative_pathologies?= Message-ID: 6,8,10or 20 micron sections should work for you? Hope this helps Cynthia James Sent from my Verizon Wireless 4G LTE Tablet -------- Original message -------- From: Tyrone Genade Date: 01/15/2015 5:56 PM (GMT-06:00) To: histonet Subject: [Histonet] preferable thickness of sections for diagnostic demonstration of neurodegenerative pathologies Hello, I am planning a study and need advice on the optimum tissue thickness for diagnosis of: alpha-synucleinopathies tauopathies amyloid plaques The study is primarily comparative distribution and frequency between age groups and would be looking to compare "affected neurons per field of vision/total neurons visible" rather than stereological cell counts (which are beyond my budget at this time). I plan on using fixed paraffin embedded tissue. The GE site ( http://www.clarientinc.com/test-menu.aspx?AlphabeticalFilter=A&TestID=2571 ) suggest 4 micron for Lewy Bodies. Any particular reason? Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> gmail.com Thu Jan 15 19:22:10 2015 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Jan 15 19:22:16 2015 Subject: [Histonet] Re: breast tissue and radioactive seeds Message-ID: Kaye (where?) inquires: We are going to be starting a new procedure here called "Conversion to Radioactive Seed Localized Breast Surgery" and I have been asked to come to this meeting. As I have been strictly in Dermatology for the past few years I am not familiar with this process. I have dealt with the prostate radioactive seeds before but not sure if this will involve the Histology lab in the form of surgical tissue or not. I would like to be as informed as possible before the meeting which is next week, so I came to the source I value the most. Can anyone share any information with me? ****************************** To which Phil Gilbert (also where?, but he has a British accent anyway): We've recently started using this procedure, so a (very) brief synopsis as follows: Rather than a wire localisation of a breast tumour (using ultrasound) where a surgeon follows the wire to excise the tumour, a tiny radioactive seed is used instead. The seed is injected by syringe into the tumour, where it is "held" by the tissue (rather than a wire which can become dislodged by movement of the patient). The surgeon then uses a fine-tuned radiation meter to locate the seed (and thus the tumour) for excision. >From theatre, the breast excision specimen is x-rayed by our Radiology Dept to confirm that tumour does not appear to be at the resection margins (if it does, then a re-excision is done). Critically, because the seeds can be placed totally within the tumour, very few immediate shave re-excisions are required - this is the great advantage. The histology lab receives the specimen, where we need to be very careful not to fracture/cut the seed. We use our own x-ray cabinet to show whereabouts in the specimen the seed is lying. Once the specimen is sliced, the seed is retrieved and the specimen trimmed as normal. Here in the UK, the main concern over this technique is that no source of radiation can be lost (down a trimming bench sink, onto the floor etc.). We therefore have a complex chain of custody record which confirms the presence of the seed by radiation monitor at all areas of the hospital. ********************************* I Googled an excellent clinical article on radioactive seed localized breast surgery, apparently widely used at more than one Mayo Clinic. James W. Jakub MD et al. (Mayo Clinic, Rochester MN). Current status of radioactive seed for localization of non palpable breast lesions. The American Journal of Surgery, Vol 199, No 4, April 2010. The article in full is accessible at http://health.usf.edu/nocms/medicine/breasthealth/PDFDocuments/Radioactive%20Seed%20Localization.pdf The review article and the comments suggest equipment (specimen x-ray machine, gamma counter) which the ordinary surgical pathology lab doesn't have access to. The "seed" contains a charge of iodine 125 (gamma emitter, half-life 60 days) which can be accidentally cut into. You may assume you'll get your first specimen without warning, just like you got your first sentinel node specimen. You need to have a pathologist staying on top of this and providing feedback, but he probably won't have the authority to do anything, and they won't send him for training. Bob Richmond Samurai Pathologist Maryville TN From rsrichmond <@t> gmail.com Thu Jan 15 19:31:16 2015 From: rsrichmond <@t> gmail.com (Bob Richmond) Date: Thu Jan 15 19:31:22 2015 Subject: [Histonet] Re: marking small samples Message-ID: Yves Heremans asks: >>Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block? Preferably something that would not interfere with subsequent antibody staining.<< Eosin supposedly interferes with subsequent fluorescence procedures such as FISH. I've seen safranin O used quite successfully for marking small specimens while grossing. Supposedly it doesn't cause the problems eosin does. Like eosin, it isn't visible in the stained sections. Safranin O (C.I. 50240) - unrelated to the natural dye saffron, of course - is used as the counterstain in the microbiologist's Gram stain, so that you can easily get a sample of the solution from the hospital laboratory. Supposedly alum hematoxylin can also be used for this purpose, but I've never seen it done. Bob Richmond Samurai Pathologist Maryville TN From akemiat3377 <@t> gmail.com Fri Jan 16 08:19:21 2015 From: akemiat3377 <@t> gmail.com (Eileen Akemi Allison) Date: Fri Jan 16 08:19:28 2015 Subject: [Histonet] P26 billing for IHC Professional component Message-ID: <9C292E89-ACCF-4E90-A5A3-CC6184F17418@gmail.com> Good morning Histoland: Happy Friday! I was asked by a friend to post this on histonet. They have a question for those of you who may be familiar with billing for the IHC professional component only. Here is the scenario; a small lab who does not perform IHC in-house sends a block to be cut and stained for IHC to a reference lab who performs a stain only IHC. The reference lab then sends the stained slides back to the ordering facility and their pathologist makes the interpretation of the stained slides. Arrangements have been made for the reference lab to charge the patients insurance company and/or patient for the technical component of the IHC stain. How would the requesting facility billing department charge for the pathologists professional component? Do they charge with a P26 CPT code only? Thank you in advance for your responses! Akemi Allison BS, HT/HTL (ASCP) President Phoenix Lab Consulting Marina, CA 93933 Tele: (408) 335-9994 Email: akemiat3377@gmail.com From HernandezJW <@t> uthscsa.edu Fri Jan 16 08:54:27 2015 From: HernandezJW <@t> uthscsa.edu (Hernandez, Jesus Willibaldo) Date: Fri Jan 16 08:54:34 2015 Subject: [Histonet] General Tissue Processing Message-ID: I had a quick question about processing bone samples. Since we do not have an automatic tissue processor, what are the recommended solutions that I can leave my samples overnight in without ruining them. Last time I processed tissue, I left the samples overnight in 80%EtOH and then in 100%EtOH. Is this acceptable or will this affect the overall tissue morphology? Thank you. Regards, Jesse H. From Joyce.Weems <@t> emoryhealthcare.org Fri Jan 16 09:57:19 2015 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Fri Jan 16 09:57:35 2015 Subject: [Histonet] P26 billing for IHC Professional component In-Reply-To: <9C292E89-ACCF-4E90-A5A3-CC6184F17418@gmail.com> References: <9C292E89-ACCF-4E90-A5A3-CC6184F17418@gmail.com> Message-ID: Yes, that is how it would be done. Just a reminder if patient is Medicare/Medicaid, the hospital must bill for the technical component as well - UNLESS the patient is from an outreach physician's office. And you cannot go exclusively by age - there are underage Medicare patients. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Eileen Akemi Allison Sent: Friday, January 16, 2015 9:19 AM To: Histonet Subject: [Histonet] P26 billing for IHC Professional component Good morning Histoland: Happy Friday! I was asked by a friend to post this on histonet. They have a question for those of you who may be familiar with billing for the IHC professional component only. Here is the scenario; a small lab who does not perform IHC in-house sends a block to be cut and stained for IHC to a reference lab who performs a stain only IHC. The reference lab then sends the stained slides back to the ordering facility and their pathologist makes the interpretation of the stained slides. Arrangements have been made for the reference lab to charge the patients insurance company and/or patient for the technical component of the IHC stain. How would the requesting facility billing department charge for the pathologists professional component? Do they charge with a P26 CPT code only? Thank you in advance for your responses! Akemi Allison BS, HT/HTL (ASCP) President Phoenix Lab Consulting Marina, CA 93933 Tele: (408) 335-9994 Email: akemiat3377@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From abadesuyi <@t> nrh-ok.com Fri Jan 16 12:04:16 2015 From: abadesuyi <@t> nrh-ok.com (Adesupo, Adesuyi (Banjo)) Date: Fri Jan 16 12:04:27 2015 Subject: [Histonet] HSV I & II control slides Message-ID: <04EE4F75BB5FB246ADB68D69B746044399095FC358@MAIL.nrhnt.nrh-ok.com> Hello, Does anyone out there have some good HSV I & II tissues they could share with us? We have been having problem with our HSV I & HSV II, because the commercially prepared positive control slides that we bought did not stain as expected. You guys are the best. Have a good weekend and thanks for your help. Best regards, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. From tgenade <@t> gmail.com Fri Jan 16 14:23:17 2015 From: tgenade <@t> gmail.com (Tyrone Genade) Date: Fri Jan 16 14:23:25 2015 Subject: [Histonet] taupathies, LB & amyloid: IHC labeling followed by staining question Message-ID: Hello, I found the following study, http://www.ncbi.nlm.nih.gov/pubmed/12000724, where sections were probed with alpha-synuclein antibodies and then developed with DAB. Then the sections were stained with Congo Red for amyloid. The afore mentioned article references http://jhc.sagepub.com/content/10/3/355 for the amyloid protocol. I assume they, the first article, had used the alkaline Congo Red method. The results look good and I can use this. Does anyone have any experience in doing such a double labeling experiment? Are there any problems that could be anticipated? If I use an alkaline phosphatase chromogen instead, could I expect problems? I am thinking of trying the MultiView kit by Enzo Life Sciences: http://www.enzolifesciences.com/ADI-950-101/multiview-mouse-hrp-mouse-ap-ihc-kit/ and perhaps using some of the more vibrant chromogens: http://www.enzolifesciences.com/platforms/immunohistochemistry/#An-Unrivaled-Selection-of-High-Definition-Chromogens . Anyone have any references to papers that spell out a protocol? From what I can make out, the slides were first developed for IHC and then transferred to NaCl saturated 80% ethanol and then to the Congo Red where after the slides were dehydrated and cleared. (I assume, I could just as well mount in an aqueous mounting medium such as glycerol or mowiol/n-propylgallate?) Thanks for the answers to my previous question. Seems 10 micron is a good middle ground for the demonstration of the various pathologies from serial and double-stained sections. Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. From liz <@t> premierlab.com Fri Jan 16 15:12:29 2015 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Jan 16 15:12:39 2015 Subject: [Histonet] taupathies, LB & amyloid: IHC labeling followed by staining question In-Reply-To: References: Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECDFC3@SBS2K8.premierlab.local> I find that when coupling IHC and special stains the best chromogen to use is DAB, unless the colors are too similar. We would traditionally run the IHC first with the DAB chromogen which is not going anywhere and then essentially counterstain with whatever special stain you are performing. Basically you run the special as you would normally after the IHC is completed. I would not switch to a AP detection system most of those chromogens are not as permanent as DAB. Brown IHC staining combined with the reddish orange from the congo red with a hematoxylin counterstain should look nice. You can then dehydrate, clear and mount in a permanent mounting media so why would you want to mount in aqueous. I would keep it simple. Good Luck. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tyrone Genade Sent: Friday, January 16, 2015 1:23 PM To: histonet Subject: [Histonet] taupathies, LB & amyloid: IHC labeling followed by staining question Hello, I found the following study, http://www.ncbi.nlm.nih.gov/pubmed/12000724, where sections were probed with alpha-synuclein antibodies and then developed with DAB. Then the sections were stained with Congo Red for amyloid. The afore mentioned article references http://jhc.sagepub.com/content/10/3/355 for the amyloid protocol. I assume they, the first article, had used the alkaline Congo Red method. The results look good and I can use this. Does anyone have any experience in doing such a double labeling experiment? Are there any problems that could be anticipated? If I use an alkaline phosphatase chromogen instead, could I expect problems? I am thinking of trying the MultiView kit by Enzo Life Sciences: http://www.enzolifesciences.com/ADI-950-101/multiview-mouse-hrp-mouse-ap-ihc-kit/ and perhaps using some of the more vibrant chromogens: http://www.enzolifesciences.com/platforms/immunohistochemistry/#An-Unrivaled-Selection-of-High-Definition-Chromogens . Anyone have any references to papers that spell out a protocol? From what I can make out, the slides were first developed for IHC and then transferred to NaCl saturated 80% ethanol and then to the Congo Red where after the slides were dehydrated and cleared. (I assume, I could just as well mount in an aqueous mounting medium such as glycerol or mowiol/n-propylgallate?) Thanks for the answers to my previous question. Seems 10 micron is a good middle ground for the demonstration of the various pathologies from serial and double-stained sections. Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor_tobias <@t> comcast.net Fri Jan 16 16:40:56 2015 From: victor_tobias <@t> comcast.net (victor_tobias@comcast.net) Date: Fri Jan 16 16:41:18 2015 Subject: [Histonet] Cytology billing In-Reply-To: <1664620598.140873.1421447782450.JavaMail.zimbra@comcast.net> Message-ID: <949194036.141793.1421448056442.JavaMail.zimbra@comcast.net> We are having debates regarding billing 88173 and 88108 on the same specimen. Padget seems to reference using 88108 when there is a second specimen, but most of our scenarios are single specimens. ? Anyone want to take a shot at it? ? Victor Sleepless in Seattle From ian.bernard <@t> comcast.net Fri Jan 16 17:02:10 2015 From: ian.bernard <@t> comcast.net (ian bernard) Date: Fri Jan 16 17:02:26 2015 Subject: [Histonet] General Tissue Processing In-Reply-To: References: Message-ID: <00b301d031e0$7635ff70$62a1fe50$@comcast.net> Fixative is best or 10 % NBF -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hernandez, Jesus Willibaldo Sent: Friday, January 16, 2015 7:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] General Tissue Processing I had a quick question about processing bone samples. Since we do not have an automatic tissue processor, what are the recommended solutions that I can leave my samples overnight in without ruining them. Last time I processed tissue, I left the samples overnight in 80%EtOH and then in 100%EtOH. Is this acceptable or will this affect the overall tissue morphology? Thank you. Regards, Jesse H. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From craigak12 <@t> gmail.com Fri Jan 16 17:27:49 2015 From: craigak12 <@t> gmail.com (Jb) Date: Fri Jan 16 17:27:59 2015 Subject: [Histonet] Cost Analysis: Message-ID: Hello Histonet, Can someone please help me with a formula to figure out cost/analysis of IHC antibody per slide, detection system per slide? I am trying to break down all of our current lab costs (even processing) and I am new at this any help is greatly appreciated. Sent from my iPhone From richard.wild <@t> wanadoo.fr Sat Jan 17 03:37:18 2015 From: richard.wild <@t> wanadoo.fr (richard wild) Date: Sat Jan 17 03:36:15 2015 Subject: [Histonet] Need : DAKO baskets / coverslipper (19 or 30 slides) Message-ID: <54BA2D4E.6030908@wanadoo.fr> Hi I badly need DAKO baskets (either 19 or 30 slides type) It is impossible for me to get them in France even at Dako. So I would really appreciate, if someone could help me (1 or 2 baskets would be good enough) (you can see the baskets - last image on that page) http://www.dssimage.com/productDesc.asp?prinid=90&prodid=842 From rjbuesa <@t> yahoo.com Sat Jan 17 12:23:24 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jan 17 12:26:24 2015 Subject: [Histonet] Cost Analysis: In-Reply-To: References: Message-ID: <1351850679.1583035.1421519004282.JavaMail.yahoo@jws10044.mail.ne1.yahoo.com> Costs have to include MATERIALS and SALARY component so you have to do several things:1- determine the "hands-on" time preparing the slide to go the IHC procedure. For example: if you do IHC automated there is time in handling the slides to complete HIER (even when the HIER time itself does not require actual handling). There is time needed to take the slides from HIER to be placed in the automated instrument, and so on, There is always a certain amount of time involved in handling the slides, even if you do IHC manually the time of incubation cannot be included because the tech can do other things while the sections are incubated. Every and all steps requiring the handling of the slides has to be known and that will be the TIME component. You then will have to determine HOW MANY SLIDES can be handled in that time frame and, depending on the hourly salary of the tech doing IHC that will be the salary component for EACH BATCH of slides. Divide the BATCH cost/number of slides = COST/SLIDE in the salary component. 2- determine the materials costs per slide: keep track of how many slides can be completed with EACH material component (either from a kit or from "lab made" reagents). If you use only kits the task is simpler because you know the cost of each kit: determine the date it was open and the date when it is used up and you have to keep track of all the slides it was used for. Add-up ALL the costa of ALL the kits and divide the TOTAL COST / ALL the slides = material cost/slide.If you prepare your own reagents or if you use Ab concentrates, you have to consider the components costs remembering to use the cost of the Ab diluents if using concentrates and, again, you have to keep track of the slides processed. At the end you add-up the cost/slide found in steps "1" and "2" and you will get an idea of the total IHC cost / slide.Consider also that in "cost/slide 1" (salary component) nothing about the "benefits" components (Social Security, insurance, vacations and any other benefits your institution incorporates into the salary) will be included. You will get the 'GROSS SALARY component/slide". In the preparation of the slides you have also to include the dewaxing?hydrating steps of the sections and its time (either manual or automated). Now, how you keep track of all these times and materials used, is up to you, but you have to develop that system that will allow you to determine, as accurately as possible, the time and materials used in preparing each finished IHC slide, that will have to include also: dehydrating?clearing?coverlipping?labelling (if not labeled at the start)?sorting?matcing with the paper work?delivering to the pathologists. EVERY task, its time and material component for EACH slide has to be determined to get an idea of your costs/slide. Under separate cover I will send you an article with the results I obtained in the lab I last worked.Ren? J. On Friday, January 16, 2015 6:28 PM, Jb wrote: Hello Histonet, Can someone please help me with a formula to figure out cost/analysis of IHC antibody per slide, detection system per slide?? I am trying to break down all of our current lab costs (even processing) and I am new at this any help is greatly appreciated. Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From joelleweaver <@t> hotmail.com Sat Jan 17 14:18:29 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Sat Jan 17 14:18:33 2015 Subject: [Histonet] Cost Analysis: In-Reply-To: References: Message-ID: I used the vendor reagent contract/proposal/organizational pricing. You need the cost for each reagent, bulk, detection, ancillaries, slides, coverslips, labels and antibody. I broke it down by each component in the per slide unit, then figured it the other way by adding each individual cost for each antibody on the menu to get a cost/IHC test for each test on the current IHC menu. I then added the overhead estimate %, instrumentation costs/maintenance/repair contract annualized, and an estimated wage/labor unit based on time and wage/hour . You can then look at these types of costs too, which could be added in to the cost/test or looked at separately. This gives a pretty good estimate of operational cost/test. I put this against the current reimburshment/test for reference. You can do the same thing with any routine process in the lab. This is just how I did it. I used excel to do the calculations. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: craigak12@gmail.com > Date: Fri, 16 Jan 2015 16:27:49 -0700 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cost Analysis: > > Hello Histonet, > > Can someone please help me with a formula to figure out cost/analysis of IHC antibody per slide, detection system per slide? > > I am trying to break down all of our current lab costs (even processing) and I am new at this any help is greatly appreciated. > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Sun Jan 18 20:01:43 2015 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Sun Jan 18 20:01:49 2015 Subject: [Histonet] Epitomics Message-ID: Hi, You will want to contact AbCam. They bought Epitomics a while ago. Their website is www.abcam.com and their support is great. Amos Message: 7 Date: Thu, 15 Jan 2015 16:50:47 -0500 From: Clare Thornton Subject: [Histonet] Epitomics To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone know if there is an Epitomics representative for the northeast (New England)? I've emailed the company but have not heard anything back. Thank you! Clare From casie4384 <@t> gmail.com Thu Jan 15 13:53:00 2015 From: casie4384 <@t> gmail.com (Casie Phillips) Date: Mon Jan 19 08:05:04 2015 Subject: [Histonet] rodent eye Message-ID: Good afternoon, I am currently working with Lewis rats performing corneal alkali injuries at varying strengths. Is there someone there that has prior experience working with a rat eye and would be willing to share information on the most effective ways to preserve, fix and cut the cornea sample. We are interested in using the cornea without using the whole globe if possible. For now we will be using basic H&E staining with a possibility of immunohistochemistry at a later time. The main outcome we are looking for is to find the presence of neutrophils in the cornea. A second objective is to look for any damaged or newly reconstructed tissue. I would greatly appreciate any advice relating to the type of paraffin used, the ideal length of time to save the tissue and any assistance you can suggest for completing this process successfully. Thank you for your time. Any assistance will be greatly appreciated. Sincerely, Casie Phillips Casie4384@gmail.com From info <@t> biopathx.com Mon Jan 19 10:55:37 2015 From: info <@t> biopathx.com (BioPathx) Date: Mon Jan 19 10:55:45 2015 Subject: [Histonet] HSV I & II control slides In-Reply-To: <04EE4F75BB5FB246ADB68D69B746044399095FC358@MAIL.nrhnt.nrh-ok.com> References: <04EE4F75BB5FB246ADB68D69B746044399095FC358@MAIL.nrhnt.nrh-ok.com> Message-ID: <1556829931.10351614.1421686537504.open-xchange@bosoxweb04.eigbox.net> We have HSV I and HSV II control slides in BioPathx. All our control slides are validated. Please visit our website at Biopathx.com or contact us at info@biopathx.com for more information. Best Regards Biopathx.com > On January 16, 2015 at 1:04 PM "Adesupo, Adesuyi (Banjo)" > wrote: > > > Hello, > Does anyone out there have some good HSV I & II tissues they could share with > us? We have been having problem with our HSV I & HSV II, because the > commercially prepared positive control slides that we bought did not stain as > expected. > You guys are the best. Have a good weekend and thanks for your help. > > > Best regards, > > Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS > Histology Supervisor > Norman Regional Health System, > Norman, OK 73071. > Tel: 405- 307- 1145 > > > ====================================== > CONFIDENTIALITY NOTICE: > > This e-mail communication and any attachments may > contain confidential and privileged information for the use > of the designated recipients named above. If you are not > the intended recipient, you are hereby notified that you > have received this communication in error and that any > review, disclosure, dissemination, distribution, or copying > of it or its contents is prohibited. If you have received > this communication in error, please notify the sender > immediately and destroy all copies of this communication > and any attachments. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From classicdoc <@t> gmail.com Mon Jan 19 12:03:46 2015 From: classicdoc <@t> gmail.com (Douglas Gregg) Date: Mon Jan 19 12:03:58 2015 Subject: [Histonet] cutting a honeybee Message-ID: It looks like I will be helping a student get started looking for the invertebrate iridovirus in honey bees that might be involved in colony collapse syndrome. Until we get up to speed, and set up a histolab in my basement, is there someone who will be willing to embed and cut a couple of normal honey bees for his histology study? I will eventually teach him how to embed and cut and later immunnostain, but we are just starting out. He will first have to learn the anatomy and histology of the bee and so will I. Doug Gregg retired veterinary pathologist From jvickroy <@t> SpringfieldClinic.com Mon Jan 19 12:28:02 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Mon Jan 19 12:28:18 2015 Subject: [Histonet] Recycling alcohol Message-ID: <9B1A1501A800064397369BD8072E6BCA96BF82@E2K10DB.springfieldclinic.com> We are planning to recycle alcohol in the new lab I am working with. Previously I always used an alcohol blend such as the "Flex" products. However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. We will be getting our alcohol from Thermofisher. Can anyone tell me which alcohols they are using for dehydration? Reagent alcohol or ethyl alcohol. Obviously we will make our own concentrations from a 100%. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From HornHV <@t> archildrens.org Mon Jan 19 13:05:11 2015 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Jan 19 13:05:23 2015 Subject: [Histonet] RE: Recycling alcohol In-Reply-To: <9B1A1501A800064397369BD8072E6BCA96BF82@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA96BF82@E2K10DB.springfieldclinic.com> Message-ID: <25A4DE08332B19499904459F00AAACB719E8D74914@EVS1.archildrens.org> With Ethyl alcohol you will need a license and will have to keep records. With reagent grade alcohol none of that is necessary. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Monday, January 19, 2015 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling alcohol We are planning to recycle alcohol in the new lab I am working with. Previously I always used an alcohol blend such as the "Flex" products. However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. We will be getting our alcohol from Thermofisher. Can anyone tell me which alcohols they are using for dehydration? Reagent alcohol or ethyl alcohol. Obviously we will make our own concentrations from a 100%. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Linda.Margraf <@t> cookchildrens.org Mon Jan 19 13:24:00 2015 From: Linda.Margraf <@t> cookchildrens.org (Linda Margraf) Date: Mon Jan 19 13:24:08 2015 Subject: [Histonet] Renewing Histonet subscription Message-ID: <928719B9EBFA1C4686918B975FF84528EE842824@CCHCSMBX04.CCHCS.LDAP> Regarding the message Alice sent below that went out to the list...... I have been the Histonet administrator since its inception and I have NEVER heard of a need to renew a subscription. I don't think the server is programmed to ask for that (at least I haven't been notified of such a thing) so I think it must be a bogus request so please ignore it. Thanks Linda M Date: Wed, 14 Jan 2015 17:45:48 -0500 From: Alice Neumann Subject: [Histonet] Message to renew subscription. To: histonet@lists.utsouthwestern.edu. Message-ID: <8D1FE62F98B19F2-21E4-9955@webmail-va036.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Got a message to renew subscription or it will be cut off.? Would like to continue receiving histonet.? Is this message from some criminal or from histonet?? Alice Neumann MD Pinnacle Pathology PC From mbruno3 <@t> buffalo.edu Mon Jan 19 13:53:01 2015 From: mbruno3 <@t> buffalo.edu (Michael Bruno) Date: Mon Jan 19 13:53:16 2015 Subject: [Histonet] Achieving Better Antibody Penetrance in my Tissue Sections (IHC) Message-ID: <54BD609D.3090309@buffalo.edu> Hi All, I'm new to graduate school and the world of immunohistochemistry. I am looking for a way to change my protocols so that the tissue sections are stained/penetrated by the antibodies more effectively. I am doing an anti-substance P stain, followed by an anti-RFP stain in 50um sections of rat brains. The rats were injected unilaterally with an AAV10 (with an mCherry tag) virus (into the dorsal striatum) that was designed by my professor. When I observed the sections on a confocal microscope, it was apparent that the center of the sections do not have the same intensity of staining that is found on the outside edges of the tissue. ========== * Here is an outline of my procedure : * Take tissue sections I am interested in (previously observed for native fluorescence) * Rinse the floating sections in ~10mL PBS 3x5 minutes * Rinse the floating sections in ~10mL 0.5% PBST (Triton X) 3x5 minutes * Block sections in 5% normal goat serum (NGS), with 0.25% PBST for 1 hour @ room temp * Incubate sections in anti-substance P (anti-SP) at a [1:400] in 2.5% NGS, in 0.25% PBST. Overnight at 4*C, shaken. * o I used a 24-well plate to hold the sections separately. Each well contained 300ul of the solution described. I had 10 sections, and some controls. So 8 sections at a time would be treated with the antibodies. * Sections rinsed in PBS 3x5 minutes * Sections incubated in secondary antibody (Ab), Alexa488 [1:1000] in 0.25% PBST for 2 hours at room temp. * The the tissue sections are rinsed in PBS 4x10 minutes to remove extra antibodies. * At this point I start the second primary incubation: anti-RFP biotin conjugated [1:2400] in 0.25% PBST, overnight in the cold room. * Rinse sections in PBS 3x5 minutes * Incubate the sections in the second secondary antibody, Cy5 [1:2000] in 0.25% PBST for 2 hours at room temp. * Rinse the sections in PBS, 3x5 minutes. * Then the sections were mounted to glass slides. Prolong Gold w/ DAPI was the mounting medium. Cover slipped. * I dried for a few days, in a desk drawer; covered and dark. ========== Thanks for reading all of this. Since it would be impossible to change the thickness of the sections (they are cut and stored in the fridge in 0.02% sodium azide), I think I need to change incubation times, temperature, and PBST concentrations. Any tips would be greatly appreciated! If you have had a similar issue in the past, I would like to know what you did to fix it. Thanks! Mike From lesliegarcia318 <@t> gmail.com Mon Jan 19 14:25:35 2015 From: lesliegarcia318 <@t> gmail.com (Leslie) Date: Mon Jan 19 14:25:52 2015 Subject: [Histonet] Achieving Better Antibody Penetrance in my Tissue Sections (IHC) In-Reply-To: <54BD609D.3090309@buffalo.edu> References: <54BD609D.3090309@buffalo.edu> Message-ID: <2B46FD29-6251-4172-B78B-B4C21663447F@gmail.com> Is there a reason you probe with the two primaries independently? I stain rat spinal cord and brain, 35 microns thick free floating, with both primary's at the same time. I stain at room temp on a shaker overnight. This seems to work pretty good. Thanks Leslie Sent from my iPhone > On Jan 19, 2015, at 11:53 AM, Michael Bruno wrote: > > Hi All, > > I'm new to graduate school and the world of immunohistochemistry. > > I am looking for a way to change my protocols so that the tissue sections are stained/penetrated by the antibodies more effectively. > > I am doing an anti-substance P stain, followed by an anti-RFP stain in 50um sections of rat brains. The rats were injected unilaterally with an AAV10 (with an mCherry tag) virus (into the dorsal striatum) that was designed by my professor. When I observed the sections on a confocal microscope, it was apparent that the center of the sections do not have the same intensity of staining that is found on the outside edges of the tissue. > > ========== > > * Here is an outline of my procedure : > * Take tissue sections I am interested in (previously observed for > native fluorescence) > * Rinse the floating sections in ~10mL PBS 3x5 minutes > * Rinse the floating sections in ~10mL 0.5% PBST (Triton X) 3x5 minutes > * Block sections in 5% normal goat serum (NGS), with 0.25% PBST for 1 > hour @ room temp > > * Incubate sections in anti-substance P (anti-SP) at a [1:400] in 2.5% > NGS, in 0.25% PBST. Overnight at 4*C, shaken. > * > o I used a 24-well plate to hold the sections separately. Each > well contained 300ul of the solution described. I had 10 > sections, and some controls. So 8 sections at a time would be > treated with the antibodies. > * Sections rinsed in PBS 3x5 minutes > * Sections incubated in secondary antibody (Ab), Alexa488 [1:1000] in > 0.25% PBST for 2 hours at room temp. > * The the tissue sections are rinsed in PBS 4x10 minutes to remove > extra antibodies. > > * At this point I start the second primary incubation: anti-RFP biotin > conjugated [1:2400] in 0.25% PBST, overnight in the cold room. > * Rinse sections in PBS 3x5 minutes > * Incubate the sections in the second secondary antibody, Cy5 [1:2000] > in 0.25% PBST for 2 hours at room temp. > * Rinse the sections in PBS, 3x5 minutes. > > * Then the sections were mounted to glass slides. Prolong Gold w/ > DAPI was the mounting medium. Cover slipped. > > * I dried for a few days, in a desk drawer; covered and dark. > > > ========== > > Thanks for reading all of this. Since it would be impossible to change the thickness of the sections (they are cut and stored in the fridge in 0.02% sodium azide), I think I need to change incubation times, temperature, and PBST concentrations. > > Any tips would be greatly appreciated! If you have had a similar issue in the past, I would like to know what you did to fix it. > > Thanks! > Mike > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr <@t> comcast.net Mon Jan 19 14:30:34 2015 From: koellingr <@t> comcast.net (koellingr@comcast.net) Date: Mon Jan 19 14:30:54 2015 Subject: [Histonet] Achieving Better Antibody Penetrance in my Tissue Sections (IHC) In-Reply-To: <54BD609D.3090309@buffalo.edu> References: <54BD609D.3090309@buffalo.edu> Message-ID: <1451954290.3675699.1421699434569.JavaMail.zimbra@comcast.net> Michael, Not sure from your explanation if your mCherry tag is less intense on the inside of sections or if you are referring to the P and RFP Alexa and Cy5 stain intensity????? If the former, not sure about that.? If the later, having done very similar thing in grad school, but with different tissue and molecule targets and different antibodies, your protocol looks very similar to what I did for years.? With exception that I extended those secondaries time-wise.? Whether right or wrong, my thinking that if it took 'overnight/shaken" for a 150kDa antibody to penetrate through 50u (which is what I used), it would take more than just 2 hours for an even bigger molecule (Ab+tag) to penetrate.? I'd try extending secondary times while keeping everything else same and look for better penetration.? Otherwise, our protocols would look nearly identical. ? Ray Koelling Lake Forest Park, WA ----- Original Message ----- From: "Michael Bruno" To: "histonet@lists.utsouthwestern.edu >> Histonet Listserve" Sent: Monday, January 19, 2015 11:53:01 AM Subject: [Histonet] Achieving Better Antibody Penetrance in my Tissue????????Sections (IHC) Hi All, I'm new to graduate school and the world of immunohistochemistry. I am looking for a way to change my protocols so that the tissue sections are stained/penetrated by the antibodies more effectively. I am doing an anti-substance P stain, followed by an anti-RFP stain in 50um sections of rat brains. The rats were injected unilaterally with an AAV10 (with an mCherry tag) virus (into the dorsal striatum) that was designed by my professor. ?When I observed the sections on a confocal microscope, it was apparent that the center of the sections do not have the same intensity of staining that is found on the outside edges of the tissue. ========== ??* Here is an outline of my procedure : ??* Take tissue sections I am interested in (previously observed for ?? ?native fluorescence) ??* Rinse the floating sections in ~10mL PBS 3x5 minutes ??* Rinse the floating sections in ~10mL 0.5% PBST (Triton X) 3x5 minutes ??* Block sections in 5% normal goat serum (NGS), with 0.25% PBST for 1 ?? ?hour @ room temp ??* Incubate sections in anti-substance P (anti-SP) at a [1:400] in 2.5% ?? ?NGS, in 0.25% PBST. ?Overnight at 4*C, shaken. ??* ?? ? ?o I used a 24-well plate to hold the sections separately. ?Each ?? ? ? ?well contained 300ul of the solution described. ?I had 10 ?? ? ? ?sections, and some controls. ?So 8 sections at a time would be ?? ? ? ?treated with the antibodies. ??* Sections rinsed in PBS 3x5 minutes ??* Sections incubated in secondary antibody (Ab), Alexa488 [1:1000] in ?? ?0.25% PBST for 2 hours at room temp. ??* The the tissue sections are rinsed in PBS 4x10 minutes to remove ?? ?extra antibodies. ??* At this point I start the second primary incubation: anti-RFP biotin ?? ?conjugated [1:2400] in 0.25% PBST, overnight in the cold room. ??* Rinse sections in PBS 3x5 minutes ??* Incubate the sections in the second secondary antibody, Cy5 [1:2000] ?? ?in 0.25% PBST for 2 hours at room temp. ??* Rinse the sections in PBS, 3x5 minutes. ??* Then the sections were mounted to glass slides. ?Prolong Gold w/ ?? ?DAPI was the mounting medium. ?Cover slipped. ??* I dried for a few days, in a desk drawer; covered and dark. ========== Thanks for reading all of this. ?Since it would be impossible to change the thickness of the sections (they are cut and stored in the fridge in 0.02% sodium azide), I think I need to change incubation times, temperature, and PBST concentrations. Any tips would be greatly appreciated! ?If you have had a similar issue in the past, I would like to know what you did to fix it. Thanks! Mike _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Jan 19 17:01:55 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 19 17:02:07 2015 Subject: [Histonet] Recycling alcohol In-Reply-To: <9B1A1501A800064397369BD8072E6BCA96BF82@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA96BF82@E2K10DB.springfieldclinic.com> Message-ID: <686898767.2078754.1421708515489.JavaMail.yahoo@jws10080.mail.ne1.yahoo.com> I used 200 proof ethanol and tried also to recycle it. After making a costs analysis I concluded it?was not worth it. Too much waste, too much electricity, too much water and only about 50-60% recovery, at beast.If you have a good ethanol source I would advise?not to embark in recycling it. Xylene (if you still use it) is another thing because it is economically viable.Ren? J.? On Monday, January 19, 2015 1:28 PM, "Vickroy, James" wrote: We are planning to recycle alcohol in the new lab I am working with.? Previously I always used an alcohol blend such as the "Flex" products.? However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend.? We will be getting our alcohol from Thermofisher.? Can anyone tell me which alcohols they are using for dehydration?? Reagent alcohol or ethyl alcohol.? Obviously we will make our own concentrations from a 100%. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois? 62703 Office:? 217-528-7541, Ext. 15121 Email:? jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Mon Jan 19 19:53:17 2015 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Mon Jan 19 19:53:37 2015 Subject: [Histonet] RE: Renewing Histonet subscription In-Reply-To: <928719B9EBFA1C4686918B975FF84528EE842824@CCHCSMBX04.CCHCS.LDAP> References: <928719B9EBFA1C4686918B975FF84528EE842824@CCHCSMBX04.CCHCS.LDAP> Message-ID: <3B2CD438E1628A41BD687E98B963B7812EBB1EA7@EMBX-CLFT4.cdc.gov> I have received notice so I would guess it is a phishing scheme. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Linda Margraf Sent: Monday, January 19, 2015 2:24 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Renewing Histonet subscription Regarding the message Alice sent below that went out to the list...... I have been the Histonet administrator since its inception and I have NEVER heard of a need to renew a subscription. I don't think the server is programmed to ask for that (at least I haven't been notified of such a thing) so I think it must be a bogus request so please ignore it. Thanks Linda M Date: Wed, 14 Jan 2015 17:45:48 -0500 From: Alice Neumann Subject: [Histonet] Message to renew subscription. To: histonet@lists.utsouthwestern.edu. Message-ID: <8D1FE62F98B19F2-21E4-9955@webmail-va036.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Got a message to renew subscription or it will be cut off.? Would like to continue receiving histonet.? Is this message from some criminal or from histonet?? Alice Neumann MD Pinnacle Pathology PC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MITCHELLJA <@t> email.chop.edu Tue Jan 20 05:42:47 2015 From: MITCHELLJA <@t> email.chop.edu (Mitchell, Janice A) Date: Tue Jan 20 05:43:47 2015 Subject: [Histonet] specials stainers Message-ID: Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell From jqb7 <@t> cdc.gov Tue Jan 20 05:58:53 2015 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue Jan 20 05:59:10 2015 Subject: [Histonet] RE: specials stainers In-Reply-To: References: Message-ID: <3B2CD438E1628A41BD687E98B963B7812EBB2248@EMBX-CLFT4.cdc.gov> Have not tried the Ventana but we are very happy with the Dako. Jeanine H. Sanders, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 jqb7@cdc.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Tue Jan 20 06:04:59 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Jan 20 06:05:14 2015 Subject: [Histonet] Re: specials stainers In-Reply-To: <3B2CD438E1628A41BD687E98B963B7812EBB2248@EMBX-CLFT4.cdc.gov> References: , <3B2CD438E1628A41BD687E98B963B7812EBB2248@EMBX-CLFT4.cdc.gov> Message-ID: <1421755498382.60536@uams.edu> We have used both and prefer the Dao hands down. They are responsive if you have questions and very good staining. We don't need to clean t after each run as it is all self contained and easy to use. Pam ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Tuesday, January 20, 2015 5:58 AM To: Mitchell, Janice A; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: specials stainers Have not tried the Ventana but we are very happy with the Dako. Jeanine H. Sanders, BS HT(ASCP), QIHC Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 1600 Clifton Road, NE MS/G-32 Atlanta, Ga 30333 404-639-3590 jqb7@cdc.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Ronald.Houston <@t> nationwidechildrens.org Tue Jan 20 07:37:10 2015 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Tue Jan 20 07:37:24 2015 Subject: [Histonet] RE: specials stainers In-Reply-To: References: Message-ID: DAKO wins hands-down, without any reservation Ronnie Houston, MS HT(ASCP)QIHC FIBMS Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=nzBUrB3Xpwhn9Ae_0-lfWwc8jW8gNVBAdeg5eQ8NYLo&s=2WCrIYyU7Xu0uhPLn4ONxykj976_p9pJcFCvv5O5K0I&e= From Joyce.Weems <@t> emoryhealthcare.org Tue Jan 20 07:58:53 2015 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Jan 20 07:59:02 2015 Subject: [Histonet] RE: specials stainers In-Reply-To: References: Message-ID: I recommend Leica. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From bcooper <@t> chla.usc.edu Tue Jan 20 08:08:11 2015 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Tue Jan 20 08:08:21 2015 Subject: [Histonet] RE: specials stainers In-Reply-To: References: , Message-ID: We have a pair of each of these in our lab right now on demo (only until I can get the paperwork signed later this week). Dako takes it hands down! Thanks, Brian Cooper, HT (ASCP) Histology Supervisor, Path & Lab Medicine Children's Hospital, Los Angeles Sent from my Galaxy S3, so please forgive any weird typos . . . -----Original Message----- From: Houston, Ronald [Ronald.Houston@nationwidechildrens.org] Received: Tuesday, 20 Jan 2015, 5:39AM To: 'Mitchell, Janice A' [MITCHELLJA@email.chop.edu]; histonet@lists.utsouthwestern.edu [histonet@lists.utsouthwestern.edu] Subject: [Histonet] RE: specials stainers DAKO wins hands-down, without any reservation Ronnie Houston, MS HT(ASCP)QIHC FIBMS Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=FGzDrZ8hK6OoO1oc9Smc5viw6E0cF__gglPkcFwC2N8&r=Sq5_V4WPe-NKHXWNZ6pAinmQolgHQEbnpaFk_iS9Rpap2gKCsp18_6Vj99Jv3oyZ&m=nzBUrB3Xpwhn9Ae_0-lfWwc8jW8gNVBAdeg5eQ8NYLo&s=2WCrIYyU7Xu0uhPLn4ONxykj976_p9pJcFCvv5O5K0I&e= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From Donna.Willis <@t> baylorhealth.edu Tue Jan 20 08:09:47 2015 From: Donna.Willis <@t> baylorhealth.edu (Willis, Donna G.) Date: Tue Jan 20 08:10:00 2015 Subject: [Histonet] RE: specials stainers In-Reply-To: References: Message-ID: <2572B4D63B62E64A8078D8BBE34D40787815EEFB@BHDASVEXML2.bhcs.pvt> Leica doesn't make a Special Stain unit. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, January 20, 2015 7:59 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: specials stainers I recommend Leica. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=qVkcpx0z4DOUphvfHjrBWLvEpNlUqjp3oKn_8GMmNSM&s=eUGarkiS-QalC5C4J623kN3sHcCEC6NENgUmeAPxdbU&e= ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=qVkcpx0z4DOUphvfHjrBWLvEpNlUqjp3oKn_8GMmNSM&s=eUGarkiS-QalC5C4J623kN3sHcCEC6NENgUmeAPxdbU&e= ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. From Joyce.Weems <@t> emoryhealthcare.org Tue Jan 20 08:13:25 2015 From: Joyce.Weems <@t> emoryhealthcare.org (Weems, Joyce K.) Date: Tue Jan 20 08:13:42 2015 Subject: [Histonet] RE: specials stainers In-Reply-To: References: Message-ID: Oops - I had IHC on the brain and didn't pay close attention! Leica does not have a dedicated special stainer - but they have a multistainer which stains specials as well as H&E. Is too slow in a busy lab tho. I very much recommend the DAKO Artisan.. It is Monday on Tuesday... right? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Weems, Joyce K. Sent: Tuesday, January 20, 2015 8:59 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: RE: specials stainers I recommend Leica. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From jqb7 <@t> cdc.gov Tue Jan 20 08:13:35 2015 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue Jan 20 08:14:07 2015 Subject: [Histonet] RE: specials stainers In-Reply-To: <2572B4D63B62E64A8078D8BBE34D40787815EEFB@BHDASVEXML2.bhcs.pvt> References: <2572B4D63B62E64A8078D8BBE34D40787815EEFB@BHDASVEXML2.bhcs.pvt> Message-ID: <3B2CD438E1628A41BD687E98B963B7812EBB23F0@EMBX-CLFT4.cdc.gov> They have the ST5020 Multistainer which has the capacity to use some of the reagent wells to do specials as well as H&E's but it is not a dedicated special stain instrument. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Willis, Donna G. Sent: Tuesday, January 20, 2015 9:10 AM To: 'Weems, Joyce K.'; 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: specials stainers Leica doesn't make a Special Stain unit. Donna Willis, HT/HTL(ASCP) Anatomic Pathology Manager Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile BaylorScottandWhite.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, January 20, 2015 7:59 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: specials stainers I recommend Leica. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=qVkcpx0z4DOUphvfHjrBWLvEpNlUqjp3oKn_8GMmNSM&s=eUGarkiS-QalC5C4J623kN3sHcCEC6NENgUmeAPxdbU&e= ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIFAg&c=qhent5lL-8Lans1hhN7NTGhSd0GBLfQfwUvzHj1D5tQ&r=holbn1kbGwnoiqK76TFBdjlG1OLqpvUtZAfF_4NbNVg&m=qVkcpx0z4DOUphvfHjrBWLvEpNlUqjp3oKn_8GMmNSM&s=eUGarkiS-QalC5C4J623kN3sHcCEC6NENgUmeAPxdbU&e= ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Tue Jan 20 08:22:17 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Jan 20 08:22:23 2015 Subject: [Histonet] RE: Recycling alcohol In-Reply-To: <25A4DE08332B19499904459F00AAACB719E8D74914@EVS1.archildrens.org> References: <9B1A1501A800064397369BD8072E6BCA96BF82@E2K10DB.springfieldclinic.com> <25A4DE08332B19499904459F00AAACB719E8D74914@EVS1.archildrens.org> Message-ID: <4c96ad5c746b4ba5ba60615893a16b48@MAIL13M2N2.ad.uams.edu> Hazel is correct if you use the pure ethanol the paperwork is horrible and the limits on what should be in the lab are very low for a normal Histology Lab. Reagent alcohol is the best way to go as it has methanol and isopropanol in it so it is undrinkable and therefore not under the same ATF rules of usage. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, January 19, 2015 1:05 PM To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol With Ethyl alcohol you will need a license and will have to keep records. With reagent grade alcohol none of that is necessary. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Monday, January 19, 2015 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling alcohol We are planning to recycle alcohol in the new lab I am working with. Previously I always used an alcohol blend such as the "Flex" products. However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. We will be getting our alcohol from Thermofisher. Can anyone tell me which alcohols they are using for dehydration? Reagent alcohol or ethyl alcohol. Obviously we will make our own concentrations from a 100%. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jvickroy <@t> SpringfieldClinic.com Tue Jan 20 09:10:08 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Tue Jan 20 09:10:18 2015 Subject: [Histonet] Decon HydeAway Message-ID: <9B1A1501A800064397369BD8072E6BCA96C365@E2K10DB.springfieldclinic.com> Anybody using Decon HydeAway for neutralization of 10% NBF. If so what are your experiences and do you dispose of the product (gel) into your solid biological waste for disposal? In the past I have used Formalex or Formalex Green. This material looks cheaper but cheaper is not always better of course. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From JRobinson <@t> pathology-associates.com Tue Jan 20 10:31:24 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Tue Jan 20 10:31:36 2015 Subject: [Histonet] RE: specials stainers In-Reply-To: References: Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8AF38@PAEXCH1.PathologyAssociates.local> I have the Leica multistainer. We use it for H & E staining only. When we attempted to set it up for Special Stains, all of the silver stains had to be made up either each run or each day. Too much hassle. Jeff Robinson, HT, HTL Sierra Pathology Lab, Clovis, CA jrobinson@pathology-associates.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, January 20, 2015 6:13 AM To: 'Mitchell, Janice A'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: specials stainers Oops - I had IHC on the brain and didn't pay close attention! Leica does not have a dedicated special stainer - but they have a multistainer which stains specials as well as H&E. Is too slow in a busy lab tho. I very much recommend the DAKO Artisan.. It is Monday on Tuesday... right? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Weems, Joyce K. Sent: Tuesday, January 20, 2015 8:59 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: RE: specials stainers I recommend Leica. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From epeters2 <@t> gmu.edu Tue Jan 20 10:35:39 2015 From: epeters2 <@t> gmu.edu (Esther C Peters) Date: Tue Jan 20 10:35:48 2015 Subject: [Histonet] Re: Recycling alcohol In-Reply-To: <4c96ad5c746b4ba5ba60615893a16b48@MAIL13M2N2.ad.uams.edu> References: <9B1A1501A800064397369BD8072E6BCA96BF82@E2K10DB.springfieldclinic.com> <25A4DE08332B19499904459F00AAACB719E8D74914@EVS1.archildrens.org>, <4c96ad5c746b4ba5ba60615893a16b48@MAIL13M2N2.ad.uams.edu> Message-ID: <1421771738729.88183@gmu.edu> American Mastertech Scientific makes Reagent Alcohol with just ethanol and isopropanol. Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epeters2@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Marcum, Pamela A Sent: Tuesday, January 20, 2015 9:22 AM To: Horn, Hazel V; 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol Hazel is correct if you use the pure ethanol the paperwork is horrible and the limits on what should be in the lab are very low for a normal Histology Lab. Reagent alcohol is the best way to go as it has methanol and isopropanol in it so it is undrinkable and therefore not under the same ATF rules of usage. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, January 19, 2015 1:05 PM To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol With Ethyl alcohol you will need a license and will have to keep records. With reagent grade alcohol none of that is necessary. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Monday, January 19, 2015 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling alcohol We are planning to recycle alcohol in the new lab I am working with. Previously I always used an alcohol blend such as the "Flex" products. However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. We will be getting our alcohol from Thermofisher. Can anyone tell me which alcohols they are using for dehydration? Reagent alcohol or ethyl alcohol. Obviously we will make our own concentrations from a 100%. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtmontana <@t> primecare.org Tue Jan 20 10:36:02 2015 From: mtmontana <@t> primecare.org (Montana, Maria) Date: Tue Jan 20 10:38:04 2015 Subject: [Histonet] FW: Iron control tissue or blocks In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F76833A3EAD3AEC5@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F76833A3EAD3AEC5@EXCHANGE2K7.staprimecare.org> Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A464DB0062@EXCHANGE2K7.staprimecare.org> ________________________________ From: Montana, Maria Sent: Wednesday, January 07, 2015 1:50 PM To: hisonet@lists.utsouthwestern.edu Subject: Iron control tissue or blocks Hi, Histoneters, I was wondering if anyone out there has some good Fe control tissue or blocks that you would be willing to trade for. We have Amyloid tissue, if you are interested. Thanks, Maria Montana HTL (ASCP) CHI St Alexius Health (701)530-6732 ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). From mucram11 <@t> comcast.net Tue Jan 20 10:39:40 2015 From: mucram11 <@t> comcast.net (Pam Marcum) Date: Tue Jan 20 10:39:54 2015 Subject: [Histonet] Re: Recycling alcohol In-Reply-To: <1421771738729.88183@gmu.edu> References: <9B1A1501A800064397369BD8072E6BCA96BF82@E2K10DB.springfieldclinic.com> <25A4DE08332B19499904459F00AAACB719E8D74914@EVS1.archildrens.org> <4c96ad5c746b4ba5ba60615893a16b48@MAIL13M2N2.ad.uams.edu> <1421771738729.88183@gmu.edu> Message-ID: <295469677.1387992.1421771980759.JavaMail.zimbra@comcast.net> The issue is of isoprpanol and/or methanol is really not the point.? The ATF requires the alcohol to be unfit for human consumption so either or both of these solutions in ethanol will render it dangerous and undrinkable.? If you do not have one or both of these in the ethanol it is taxed and therefore very expensive and time consuming. ? Pam ----- Original Message ----- From: "Esther C Peters" To: "Pamela A Marcum" , "Hazel V Horn" , "James' 'Vickroy" , "Histonet" Sent: Tuesday, January 20, 2015 10:35:39 AM Subject: [Histonet] Re: Recycling alcohol American Mastertech Scientific makes Reagent Alcohol with just ethanol and isopropanol. Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epeters2@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Marcum, Pamela A Sent: Tuesday, January 20, 2015 9:22 AM To: Horn, Hazel V; 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol Hazel is correct if you use the pure ethanol the paperwork is horrible and the limits on what should be in the lab are very low for a normal Histology Lab. ?Reagent alcohol is the best way to go as it has methanol and isopropanol in it so it is undrinkable and therefore not under the same ATF rules of usage. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, January 19, 2015 1:05 PM To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol With Ethyl alcohol you will need a license and will have to keep records. ?With reagent grade alcohol none of that is necessary. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Monday, January 19, 2015 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling alcohol We are planning to recycle alcohol in the new lab I am working with. ? Previously I always used an alcohol blend such as the "Flex" products. ?However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. ? We will be getting our alcohol from Thermofisher. ?Can anyone tell me which alcohols they are using for dehydration? ?Reagent alcohol or ethyl alcohol. ?Obviously we will make our own concentrations from a 100%. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois ?62703 Office: ?217-528-7541, Ext. 15121 Email: ?jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PAMarcum <@t> uams.edu Tue Jan 20 10:51:22 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Tue Jan 20 10:51:32 2015 Subject: [Histonet] RE: specials stainers In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D8AF38@PAEXCH1.PathologyAssociates.local> References: <204A03EB5A7F0A4BB1EEDD52A963829C16D8AF38@PAEXCH1.PathologyAssociates.local> Message-ID: <09c58db6864242c2a9f4371873e68265@MAIL13M2N2.ad.uams.edu> We use our Leica H&E stainer for PAS only as it is the fastest way we have found to get 30 to 40 bone marrow PAS slides out before 6:30AM every morning. Otherwise it was just too cumbersome and the Dako does a great job on all routine stains. We only do the neuro stains and Copper by hand now. The Dako Copper kit is a different procedure and our pathologist like so it can't be used. The PAS is just too slow for the turnaround we need daily. The stain is great!! Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Tuesday, January 20, 2015 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: specials stainers I have the Leica multistainer. We use it for H & E staining only. When we attempted to set it up for Special Stains, all of the silver stains had to be made up either each run or each day. Too much hassle. Jeff Robinson, HT, HTL Sierra Pathology Lab, Clovis, CA jrobinson@pathology-associates.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, January 20, 2015 6:13 AM To: 'Mitchell, Janice A'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: specials stainers Oops - I had IHC on the brain and didn't pay close attention! Leica does not have a dedicated special stainer - but they have a multistainer which stains specials as well as H&E. Is too slow in a busy lab tho. I very much recommend the DAKO Artisan.. It is Monday on Tuesday... right? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Weems, Joyce K. Sent: Tuesday, January 20, 2015 8:59 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: RE: specials stainers I recommend Leica. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Julia.Cates <@t> AHSS.ORG Tue Jan 20 12:15:16 2015 From: Julia.Cates <@t> AHSS.ORG (Cates, Julia) Date: Tue Jan 20 12:15:23 2015 Subject: [Histonet] RE: Decon HydeAway Message-ID: <2EF522B30254BA4B9080B8AA40FDD94DB876F5C0@LKMVXCHMB32.ADVENTISTCORP.NET> Jim, We have used the HydeAway powder for several years now. We use it to neutralize tissue before disposing. Once the formalin "gels" the container is disposed of in a biohazard bag. In our experience, if there is anything other than formalin in the container (Our Dr's use Disect Aid for lymph nodes) in will not "gel" properly. In my opinion it is a very good product. Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Florida Hospital Waterman (352) 253-3333 ext.4346 | Fax: (352) 253-3592 Confidentiality Statement: This email message, including any attachments, is for the sole?use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited.? If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. Message: 2 Date: Tue, 20 Jan 2015 15:10:08 +0000 From: "Vickroy, James" Subject: [Histonet] Decon HydeAway To: "histonet@lists.utsouthwestern.edu" Message-ID: <9B1A1501A800064397369BD8072E6BCA96C365@E2K10DB.springfieldclinic.com> Content-Type: text/plain; charset="us-ascii" Anybody using Decon HydeAway for neutralization of 10% NBF. If so what are your experiences and do you dispose of the product (gel) into your solid biological waste for disposal? In the past I have used Formalex or Formalex Green. This material looks cheaper but cheaper is not always better of course. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From bcdukes <@t> lexhealth.org Tue Jan 20 12:27:57 2015 From: bcdukes <@t> lexhealth.org (Blake Taylor) Date: Tue Jan 20 12:28:08 2015 Subject: [Histonet] Special Stainer In-Reply-To: <201501201801.t0KI1BT8003710@rly04h.srv.mailcontrol.com> References: <201501201801.t0KI1BT8003710@rly04h.srv.mailcontrol.com> Message-ID: We previously had the Ventana Nexus which was excellent, preferred it greatly over Dako but now we moved to the Ventana Benchmark Special Stainer and it has been nothing but trouble. I honestly at this point would not recommend the Benchmark. It needs improvement. Blake Taylor Surgical Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, January 20, 2015 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 134, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Recycling alcohol (Marcum, Pamela A) 2. Decon HydeAway (Vickroy, James) 3. RE: specials stainers (Jeffrey Robinson) 4. Re: Recycling alcohol (Esther C Peters) 5. FW: Iron control tissue or blocks (Montana, Maria) 6. Re: Re: Recycling alcohol (Pam Marcum) 7. RE: specials stainers (Marcum, Pamela A) ---------------------------------------------------------------------- Message: 1 Date: Tue, 20 Jan 2015 14:22:17 +0000 From: "Marcum, Pamela A" Subject: [Histonet] RE: Recycling alcohol To: "Horn, Hazel V" , "'Vickroy, James'" , "histonet@lists.utsouthwestern.edu" Message-ID: <4c96ad5c746b4ba5ba60615893a16b48@MAIL13M2N2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" Hazel is correct if you use the pure ethanol the paperwork is horrible and the limits on what should be in the lab are very low for a normal Histology Lab. Reagent alcohol is the best way to go as it has methanol and isopropanol in it so it is undrinkable and therefore not under the same ATF rules of usage. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, January 19, 2015 1:05 PM To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol With Ethyl alcohol you will need a license and will have to keep records. With reagent grade alcohol none of that is necessary. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Monday, January 19, 2015 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling alcohol We are planning to recycle alcohol in the new lab I am working with. Previously I always used an alcohol blend such as the "Flex" products. However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. We will be getting our alcohol from Thermofisher. Can anyone tell me which alcohols they are using for dehydration? Reagent alcohol or ethyl alcohol. Obviously we will make our own concentrations from a 100%. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 2 Date: Tue, 20 Jan 2015 15:10:08 +0000 From: "Vickroy, James" Subject: [Histonet] Decon HydeAway To: "histonet@lists.utsouthwestern.edu" Message-ID: <9B1A1501A800064397369BD8072E6BCA96C365@E2K10DB.springfieldclinic.com> Content-Type: text/plain; charset="us-ascii" Anybody using Decon HydeAway for neutralization of 10% NBF. If so what are your experiences and do you dispose of the product (gel) into your solid biological waste for disposal? In the past I have used Formalex or Formalex Green. This material looks cheaper but cheaper is not always better of course. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ------------------------------ Message: 3 Date: Tue, 20 Jan 2015 16:31:24 +0000 From: Jeffrey Robinson Subject: [Histonet] RE: specials stainers To: "histonet@lists.utsouthwestern.edu" Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8AF38@PAEXCH1.PathologyAssociates.local> Content-Type: text/plain; charset="us-ascii" I have the Leica multistainer. We use it for H & E staining only. When we attempted to set it up for Special Stains, all of the silver stains had to be made up either each run or each day. Too much hassle. Jeff Robinson, HT, HTL Sierra Pathology Lab, Clovis, CA jrobinson@pathology-associates.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, January 20, 2015 6:13 AM To: 'Mitchell, Janice A'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: specials stainers Oops - I had IHC on the brain and didn't pay close attention! Leica does not have a dedicated special stainer - but they have a multistainer which stains specials as well as H&E. Is too slow in a busy lab tho. I very much recommend the DAKO Artisan.. It is Monday on Tuesday... right? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Weems, Joyce K. Sent: Tuesday, January 20, 2015 8:59 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: RE: specials stainers I recommend Leica. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ------------------------------ Message: 4 Date: Tue, 20 Jan 2015 16:35:39 +0000 From: Esther C Peters Subject: [Histonet] Re: Recycling alcohol To: "Marcum, Pamela A" , "Horn, Hazel V" , "'Vickroy, James'" , "histonet@lists.utsouthwestern.edu" Message-ID: <1421771738729.88183@gmu.edu> Content-Type: text/plain; charset="iso-8859-1" American Mastertech Scientific makes Reagent Alcohol with just ethanol and isopropanol. Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epeters2@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Marcum, Pamela A Sent: Tuesday, January 20, 2015 9:22 AM To: Horn, Hazel V; 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol Hazel is correct if you use the pure ethanol the paperwork is horrible and the limits on what should be in the lab are very low for a normal Histology Lab. Reagent alcohol is the best way to go as it has methanol and isopropanol in it so it is undrinkable and therefore not under the same ATF rules of usage. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, January 19, 2015 1:05 PM To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol With Ethyl alcohol you will need a license and will have to keep records. With reagent grade alcohol none of that is necessary. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Monday, January 19, 2015 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling alcohol We are planning to recycle alcohol in the new lab I am working with. Previously I always used an alcohol blend such as the "Flex" products. However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. We will be getting our alcohol from Thermofisher. Can anyone tell me which alcohols they are using for dehydration? Reagent alcohol or ethyl alcohol. Obviously we will make our own concentrations from a 100%. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 20 Jan 2015 10:36:02 -0600 From: "Montana, Maria" Subject: [Histonet] FW: Iron control tissue or blocks To: "histonet@lists.utsouthwestern.edu" Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A464DB0062@EXCHANGE2K7.staprimecare.org> Content-Type: text/plain; charset="iso-8859-1" ________________________________ From: Montana, Maria Sent: Wednesday, January 07, 2015 1:50 PM To: hisonet@lists.utsouthwestern.edu Subject: Iron control tissue or blocks Hi, Histoneters, I was wondering if anyone out there has some good Fe control tissue or blocks that you would be willing to trade for. We have Amyloid tissue, if you are interested. Thanks, Maria Montana HTL (ASCP) CHI St Alexius Health (701)530-6732 ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). ------------------------------ Message: 6 Date: Tue, 20 Jan 2015 16:39:40 +0000 (UTC) From: Pam Marcum Subject: Re: [Histonet] Re: Recycling alcohol To: Esther C Peters Cc: Hazel V Horn , James' 'Vickroy , "Marcum, Pamela" , Histonet Message-ID: <295469677.1387992.1421771980759.JavaMail.zimbra@comcast.net> Content-Type: text/plain; charset=utf-8 The issue is of isoprpanol and/or methanol is really not the point.?? The ATF requires the alcohol to be unfit for human consumption so either or both of these solutions in ethanol will render it dangerous and undrinkable.?? If you do not have one or both of these in the ethanol it is taxed and therefore very expensive and time consuming. ?? Pam ----- Original Message ----- From: "Esther C Peters" To: "Pamela A Marcum" , "Hazel V Horn" , "James' 'Vickroy" , "Histonet" Sent: Tuesday, January 20, 2015 10:35:39 AM Subject: [Histonet] Re: Recycling alcohol American Mastertech Scientific makes Reagent Alcohol with just ethanol and isopropanol. Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epeters2@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Marcum, Pamela A Sent: Tuesday, January 20, 2015 9:22 AM To: Horn, Hazel V; 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol Hazel is correct if you use the pure ethanol the paperwork is horrible and the limits on what should be in the lab are very low for a normal Histology Lab. ??Reagent alcohol is the best way to go as it has methanol and isopropanol in it so it is undrinkable and therefore not under the same ATF rules of usage. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, January 19, 2015 1:05 PM To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol With Ethyl alcohol you will need a license and will have to keep records. ??With reagent grade alcohol none of that is necessary. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Monday, January 19, 2015 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling alcohol We are planning to recycle alcohol in the new lab I am working with. ?? Previously I always used an alcohol blend such as the "Flex" products. ??However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. ?? We will be getting our alcohol from Thermofisher. ??Can anyone tell me which alcohols they are using for dehydration? ??Reagent alcohol or ethyl alcohol. ??Obviously we will make our own concentrations from a 100%. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois ??62703 Office: ??217-528-7541, Ext. 15121 Email: ??jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 20 Jan 2015 16:51:22 +0000 From: "Marcum, Pamela A" Subject: [Histonet] RE: specials stainers To: "'Jeffrey Robinson'" , "histonet@lists.utsouthwestern.edu" Message-ID: <09c58db6864242c2a9f4371873e68265@MAIL13M2N2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" We use our Leica H&E stainer for PAS only as it is the fastest way we have found to get 30 to 40 bone marrow PAS slides out before 6:30AM every morning. Otherwise it was just too cumbersome and the Dako does a great job on all routine stains. We only do the neuro stains and Copper by hand now. The Dako Copper kit is a different procedure and our pathologist like so it can't be used. The PAS is just too slow for the turnaround we need daily. The stain is great!! Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Tuesday, January 20, 2015 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: specials stainers I have the Leica multistainer. We use it for H & E staining only. When we attempted to set it up for Special Stains, all of the silver stains had to be made up either each run or each day. Too much hassle. Jeff Robinson, HT, HTL Sierra Pathology Lab, Clovis, CA jrobinson@pathology-associates.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, January 20, 2015 6:13 AM To: 'Mitchell, Janice A'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: specials stainers Oops - I had IHC on the brain and didn't pay close attention! Leica does not have a dedicated special stainer - but they have a multistainer which stains specials as well as H&E. Is too slow in a busy lab tho. I very much recommend the DAKO Artisan.. It is Monday on Tuesday... right? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Weems, Joyce K. Sent: Tuesday, January 20, 2015 8:59 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: RE: specials stainers I recommend Leica. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 134, Issue 23 ***************************************** PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. From raestask <@t> grics.net Tue Jan 20 12:52:05 2015 From: raestask <@t> grics.net (raestask@grics.net) Date: Tue Jan 20 12:52:17 2015 Subject: [Histonet] TIA-1 In-Reply-To: <97780233.16283405.1421779717267.JavaMail.root@grics.net> Message-ID: <1060663537.16285828.1421779925696.JavaMail.root@grics.net> Looking for protocol to use on Ventana Ultra for TIA-1. Thanks in advance. Rae Staskiewicz??? UnityPoint Health Methodist, Peoria From JRobinson <@t> pathology-associates.com Tue Jan 20 13:03:14 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Tue Jan 20 13:03:42 2015 Subject: [Histonet] RE: Special Stainer In-Reply-To: References: <201501201801.t0KI1BT8003710@rly04h.srv.mailcontrol.com> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8AF91@PAEXCH1.PathologyAssociates.local> I must agree with Blake on the BM Special Stainer. I have had one for over a year and have had many problems with it especially trying to run bone marrow retics. Ventana claims a software upgrade will be out in the 2nd quarter so I hope to see improvement. Currently, I still use the old Nexes which is excellent! Jeff Robinson, HT, HTL, Senior Histotechnologist Sierra Pathology Lab, Clovis, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blake Taylor Sent: Tuesday, January 20, 2015 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stainer We previously had the Ventana Nexus which was excellent, preferred it greatly over Dako but now we moved to the Ventana Benchmark Special Stainer and it has been nothing but trouble. I honestly at this point would not recommend the Benchmark. It needs improvement. Blake Taylor Surgical Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, January 20, 2015 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 134, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Recycling alcohol (Marcum, Pamela A) 2. Decon HydeAway (Vickroy, James) 3. RE: specials stainers (Jeffrey Robinson) 4. Re: Recycling alcohol (Esther C Peters) 5. FW: Iron control tissue or blocks (Montana, Maria) 6. Re: Re: Recycling alcohol (Pam Marcum) 7. RE: specials stainers (Marcum, Pamela A) ---------------------------------------------------------------------- Message: 1 Date: Tue, 20 Jan 2015 14:22:17 +0000 From: "Marcum, Pamela A" Subject: [Histonet] RE: Recycling alcohol To: "Horn, Hazel V" , "'Vickroy, James'" , "histonet@lists.utsouthwestern.edu" Message-ID: <4c96ad5c746b4ba5ba60615893a16b48@MAIL13M2N2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" Hazel is correct if you use the pure ethanol the paperwork is horrible and the limits on what should be in the lab are very low for a normal Histology Lab. Reagent alcohol is the best way to go as it has methanol and isopropanol in it so it is undrinkable and therefore not under the same ATF rules of usage. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, January 19, 2015 1:05 PM To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol With Ethyl alcohol you will need a license and will have to keep records. With reagent grade alcohol none of that is necessary. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Monday, January 19, 2015 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling alcohol We are planning to recycle alcohol in the new lab I am working with. Previously I always used an alcohol blend such as the "Flex" products. However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. We will be getting our alcohol from Thermofisher. Can anyone tell me which alcohols they are using for dehydration? Reagent alcohol or ethyl alcohol. Obviously we will make our own concentrations from a 100%. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 2 Date: Tue, 20 Jan 2015 15:10:08 +0000 From: "Vickroy, James" Subject: [Histonet] Decon HydeAway To: "histonet@lists.utsouthwestern.edu" Message-ID: <9B1A1501A800064397369BD8072E6BCA96C365@E2K10DB.springfieldclinic.com> Content-Type: text/plain; charset="us-ascii" Anybody using Decon HydeAway for neutralization of 10% NBF. If so what are your experiences and do you dispose of the product (gel) into your solid biological waste for disposal? In the past I have used Formalex or Formalex Green. This material looks cheaper but cheaper is not always better of course. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ------------------------------ Message: 3 Date: Tue, 20 Jan 2015 16:31:24 +0000 From: Jeffrey Robinson Subject: [Histonet] RE: specials stainers To: "histonet@lists.utsouthwestern.edu" Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8AF38@PAEXCH1.PathologyAssociates.local> Content-Type: text/plain; charset="us-ascii" I have the Leica multistainer. We use it for H & E staining only. When we attempted to set it up for Special Stains, all of the silver stains had to be made up either each run or each day. Too much hassle. Jeff Robinson, HT, HTL Sierra Pathology Lab, Clovis, CA jrobinson@pathology-associates.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, January 20, 2015 6:13 AM To: 'Mitchell, Janice A'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: specials stainers Oops - I had IHC on the brain and didn't pay close attention! Leica does not have a dedicated special stainer - but they have a multistainer which stains specials as well as H&E. Is too slow in a busy lab tho. I very much recommend the DAKO Artisan.. It is Monday on Tuesday... right? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Weems, Joyce K. Sent: Tuesday, January 20, 2015 8:59 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: RE: specials stainers I recommend Leica. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ------------------------------ Message: 4 Date: Tue, 20 Jan 2015 16:35:39 +0000 From: Esther C Peters Subject: [Histonet] Re: Recycling alcohol To: "Marcum, Pamela A" , "Horn, Hazel V" , "'Vickroy, James'" , "histonet@lists.utsouthwestern.edu" Message-ID: <1421771738729.88183@gmu.edu> Content-Type: text/plain; charset="iso-8859-1" American Mastertech Scientific makes Reagent Alcohol with just ethanol and isopropanol. Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epeters2@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Marcum, Pamela A Sent: Tuesday, January 20, 2015 9:22 AM To: Horn, Hazel V; 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol Hazel is correct if you use the pure ethanol the paperwork is horrible and the limits on what should be in the lab are very low for a normal Histology Lab. Reagent alcohol is the best way to go as it has methanol and isopropanol in it so it is undrinkable and therefore not under the same ATF rules of usage. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, January 19, 2015 1:05 PM To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol With Ethyl alcohol you will need a license and will have to keep records. With reagent grade alcohol none of that is necessary. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Monday, January 19, 2015 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling alcohol We are planning to recycle alcohol in the new lab I am working with. Previously I always used an alcohol blend such as the "Flex" products. However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. We will be getting our alcohol from Thermofisher. Can anyone tell me which alcohols they are using for dehydration? Reagent alcohol or ethyl alcohol. Obviously we will make our own concentrations from a 100%. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 20 Jan 2015 10:36:02 -0600 From: "Montana, Maria" Subject: [Histonet] FW: Iron control tissue or blocks To: "histonet@lists.utsouthwestern.edu" Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A464DB0062@EXCHANGE2K7.staprimecare.org> Content-Type: text/plain; charset="iso-8859-1" ________________________________ From: Montana, Maria Sent: Wednesday, January 07, 2015 1:50 PM To: hisonet@lists.utsouthwestern.edu Subject: Iron control tissue or blocks Hi, Histoneters, I was wondering if anyone out there has some good Fe control tissue or blocks that you would be willing to trade for. We have Amyloid tissue, if you are interested. Thanks, Maria Montana HTL (ASCP) CHI St Alexius Health (701)530-6732 ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). ------------------------------ Message: 6 Date: Tue, 20 Jan 2015 16:39:40 +0000 (UTC) From: Pam Marcum Subject: Re: [Histonet] Re: Recycling alcohol To: Esther C Peters Cc: Hazel V Horn , James' 'Vickroy , "Marcum, Pamela" , Histonet Message-ID: <295469677.1387992.1421771980759.JavaMail.zimbra@comcast.net> Content-Type: text/plain; charset=utf-8 The issue is of isoprpanol and/or methanol is really not the point.?? The ATF requires the alcohol to be unfit for human consumption so either or both of these solutions in ethanol will render it dangerous and undrinkable.?? If you do not have one or both of these in the ethanol it is taxed and therefore very expensive and time consuming. ?? Pam ----- Original Message ----- From: "Esther C Peters" To: "Pamela A Marcum" , "Hazel V Horn" , "James' 'Vickroy" , "Histonet" Sent: Tuesday, January 20, 2015 10:35:39 AM Subject: [Histonet] Re: Recycling alcohol American Mastertech Scientific makes Reagent Alcohol with just ethanol and isopropanol. Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epeters2@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Marcum, Pamela A Sent: Tuesday, January 20, 2015 9:22 AM To: Horn, Hazel V; 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol Hazel is correct if you use the pure ethanol the paperwork is horrible and the limits on what should be in the lab are very low for a normal Histology Lab. ??Reagent alcohol is the best way to go as it has methanol and isopropanol in it so it is undrinkable and therefore not under the same ATF rules of usage. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, January 19, 2015 1:05 PM To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol With Ethyl alcohol you will need a license and will have to keep records. ??With reagent grade alcohol none of that is necessary. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Monday, January 19, 2015 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling alcohol We are planning to recycle alcohol in the new lab I am working with. ?? Previously I always used an alcohol blend such as the "Flex" products. ??However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. ?? We will be getting our alcohol from Thermofisher. ??Can anyone tell me which alcohols they are using for dehydration? ??Reagent alcohol or ethyl alcohol. ??Obviously we will make our own concentrations from a 100%. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois ??62703 Office: ??217-528-7541, Ext. 15121 Email: ??jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 20 Jan 2015 16:51:22 +0000 From: "Marcum, Pamela A" Subject: [Histonet] RE: specials stainers To: "'Jeffrey Robinson'" , "histonet@lists.utsouthwestern.edu" Message-ID: <09c58db6864242c2a9f4371873e68265@MAIL13M2N2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" We use our Leica H&E stainer for PAS only as it is the fastest way we have found to get 30 to 40 bone marrow PAS slides out before 6:30AM every morning. Otherwise it was just too cumbersome and the Dako does a great job on all routine stains. We only do the neuro stains and Copper by hand now. The Dako Copper kit is a different procedure and our pathologist like so it can't be used. The PAS is just too slow for the turnaround we need daily. The stain is great!! Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Tuesday, January 20, 2015 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: specials stainers I have the Leica multistainer. We use it for H & E staining only. When we attempted to set it up for Special Stains, all of the silver stains had to be made up either each run or each day. Too much hassle. Jeff Robinson, HT, HTL Sierra Pathology Lab, Clovis, CA jrobinson@pathology-associates.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, January 20, 2015 6:13 AM To: 'Mitchell, Janice A'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: specials stainers Oops - I had IHC on the brain and didn't pay close attention! Leica does not have a dedicated special stainer - but they have a multistainer which stains specials as well as H&E. Is too slow in a busy lab tho. I very much recommend the DAKO Artisan.. It is Monday on Tuesday... right? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Weems, Joyce K. Sent: Tuesday, January 20, 2015 8:59 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: RE: specials stainers I recommend Leica. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 134, Issue 23 ***************************************** PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Tue Jan 20 13:13:14 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Tue Jan 20 13:13:25 2015 Subject: [Histonet] Electronic monitoring systems Message-ID: Hi all, I was wondering - a little while ago there was mention on the blog of equipment (processors) being monitored and alerting the 'manager' if they went down. I'm sorry I cannot remember what system this was. What do you all use? Also, what temperature control monitor is everyone using? Ours has flaws and we are gathering information. Can the two systems be combined under one company? Any help is appreciated. Thank you! Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com From jqb7 <@t> cdc.gov Tue Jan 20 13:12:51 2015 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue Jan 20 13:14:05 2015 Subject: [Histonet] RE: Special Stainer In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D8AF91@PAEXCH1.PathologyAssociates.local> References: <201501201801.t0KI1BT8003710@rly04h.srv.mailcontrol.com> <204A03EB5A7F0A4BB1EEDD52A963829C16D8AF91@PAEXCH1.PathologyAssociates.local> Message-ID: <3B2CD438E1628A41BD687E98B963B7812EBB2773@EMBX-CLFT4.cdc.gov> At the time we were looking to buy our first ss instrument we were very interested in the Nexes but they did not have a Gram protocol at the time. We went with the Dako Artisan because of this and have had good success with it. Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Tuesday, January 20, 2015 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Special Stainer I must agree with Blake on the BM Special Stainer. I have had one for over a year and have had many problems with it especially trying to run bone marrow retics. Ventana claims a software upgrade will be out in the 2nd quarter so I hope to see improvement. Currently, I still use the old Nexes which is excellent! Jeff Robinson, HT, HTL, Senior Histotechnologist Sierra Pathology Lab, Clovis, CA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blake Taylor Sent: Tuesday, January 20, 2015 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stainer We previously had the Ventana Nexus which was excellent, preferred it greatly over Dako but now we moved to the Ventana Benchmark Special Stainer and it has been nothing but trouble. I honestly at this point would not recommend the Benchmark. It needs improvement. Blake Taylor Surgical Pathology Supervisor -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, January 20, 2015 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 134, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Recycling alcohol (Marcum, Pamela A) 2. Decon HydeAway (Vickroy, James) 3. RE: specials stainers (Jeffrey Robinson) 4. Re: Recycling alcohol (Esther C Peters) 5. FW: Iron control tissue or blocks (Montana, Maria) 6. Re: Re: Recycling alcohol (Pam Marcum) 7. RE: specials stainers (Marcum, Pamela A) ---------------------------------------------------------------------- Message: 1 Date: Tue, 20 Jan 2015 14:22:17 +0000 From: "Marcum, Pamela A" Subject: [Histonet] RE: Recycling alcohol To: "Horn, Hazel V" , "'Vickroy, James'" , "histonet@lists.utsouthwestern.edu" Message-ID: <4c96ad5c746b4ba5ba60615893a16b48@MAIL13M2N2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" Hazel is correct if you use the pure ethanol the paperwork is horrible and the limits on what should be in the lab are very low for a normal Histology Lab. Reagent alcohol is the best way to go as it has methanol and isopropanol in it so it is undrinkable and therefore not under the same ATF rules of usage. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, January 19, 2015 1:05 PM To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol With Ethyl alcohol you will need a license and will have to keep records. With reagent grade alcohol none of that is necessary. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Monday, January 19, 2015 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling alcohol We are planning to recycle alcohol in the new lab I am working with. Previously I always used an alcohol blend such as the "Flex" products. However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. We will be getting our alcohol from Thermofisher. Can anyone tell me which alcohols they are using for dehydration? Reagent alcohol or ethyl alcohol. Obviously we will make our own concentrations from a 100%. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 2 Date: Tue, 20 Jan 2015 15:10:08 +0000 From: "Vickroy, James" Subject: [Histonet] Decon HydeAway To: "histonet@lists.utsouthwestern.edu" Message-ID: <9B1A1501A800064397369BD8072E6BCA96C365@E2K10DB.springfieldclinic.com> Content-Type: text/plain; charset="us-ascii" Anybody using Decon HydeAway for neutralization of 10% NBF. If so what are your experiences and do you dispose of the product (gel) into your solid biological waste for disposal? In the past I have used Formalex or Formalex Green. This material looks cheaper but cheaper is not always better of course. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ------------------------------ Message: 3 Date: Tue, 20 Jan 2015 16:31:24 +0000 From: Jeffrey Robinson Subject: [Histonet] RE: specials stainers To: "histonet@lists.utsouthwestern.edu" Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8AF38@PAEXCH1.PathologyAssociates.local> Content-Type: text/plain; charset="us-ascii" I have the Leica multistainer. We use it for H & E staining only. When we attempted to set it up for Special Stains, all of the silver stains had to be made up either each run or each day. Too much hassle. Jeff Robinson, HT, HTL Sierra Pathology Lab, Clovis, CA jrobinson@pathology-associates.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, January 20, 2015 6:13 AM To: 'Mitchell, Janice A'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: specials stainers Oops - I had IHC on the brain and didn't pay close attention! Leica does not have a dedicated special stainer - but they have a multistainer which stains specials as well as H&E. Is too slow in a busy lab tho. I very much recommend the DAKO Artisan.. It is Monday on Tuesday... right? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Weems, Joyce K. Sent: Tuesday, January 20, 2015 8:59 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: RE: specials stainers I recommend Leica. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ------------------------------ Message: 4 Date: Tue, 20 Jan 2015 16:35:39 +0000 From: Esther C Peters Subject: [Histonet] Re: Recycling alcohol To: "Marcum, Pamela A" , "Horn, Hazel V" , "'Vickroy, James'" , "histonet@lists.utsouthwestern.edu" Message-ID: <1421771738729.88183@gmu.edu> Content-Type: text/plain; charset="iso-8859-1" American Mastertech Scientific makes Reagent Alcohol with just ethanol and isopropanol. Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epeters2@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Marcum, Pamela A Sent: Tuesday, January 20, 2015 9:22 AM To: Horn, Hazel V; 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol Hazel is correct if you use the pure ethanol the paperwork is horrible and the limits on what should be in the lab are very low for a normal Histology Lab. Reagent alcohol is the best way to go as it has methanol and isopropanol in it so it is undrinkable and therefore not under the same ATF rules of usage. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, January 19, 2015 1:05 PM To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol With Ethyl alcohol you will need a license and will have to keep records. With reagent grade alcohol none of that is necessary. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Monday, January 19, 2015 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling alcohol We are planning to recycle alcohol in the new lab I am working with. Previously I always used an alcohol blend such as the "Flex" products. However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. We will be getting our alcohol from Thermofisher. Can anyone tell me which alcohols they are using for dehydration? Reagent alcohol or ethyl alcohol. Obviously we will make our own concentrations from a 100%. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Tue, 20 Jan 2015 10:36:02 -0600 From: "Montana, Maria" Subject: [Histonet] FW: Iron control tissue or blocks To: "histonet@lists.utsouthwestern.edu" Message-ID: <1E0E2B14C709174B8AC2BE0AE7F76833A464DB0062@EXCHANGE2K7.staprimecare.org> Content-Type: text/plain; charset="iso-8859-1" ________________________________ From: Montana, Maria Sent: Wednesday, January 07, 2015 1:50 PM To: hisonet@lists.utsouthwestern.edu Subject: Iron control tissue or blocks Hi, Histoneters, I was wondering if anyone out there has some good Fe control tissue or blocks that you would be willing to trade for. We have Amyloid tissue, if you are interested. Thanks, Maria Montana HTL (ASCP) CHI St Alexius Health (701)530-6732 ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). ------------------------------ Message: 6 Date: Tue, 20 Jan 2015 16:39:40 +0000 (UTC) From: Pam Marcum Subject: Re: [Histonet] Re: Recycling alcohol To: Esther C Peters Cc: Hazel V Horn , James' 'Vickroy , "Marcum, Pamela" , Histonet Message-ID: <295469677.1387992.1421771980759.JavaMail.zimbra@comcast.net> Content-Type: text/plain; charset=utf-8 The issue is of isoprpanol and/or methanol is really not the point.?? The ATF requires the alcohol to be unfit for human consumption so either or both of these solutions in ethanol will render it dangerous and undrinkable.?? If you do not have one or both of these in the ethanol it is taxed and therefore very expensive and time consuming. ?? Pam ----- Original Message ----- From: "Esther C Peters" To: "Pamela A Marcum" , "Hazel V Horn" , "James' 'Vickroy" , "Histonet" Sent: Tuesday, January 20, 2015 10:35:39 AM Subject: [Histonet] Re: Recycling alcohol American Mastertech Scientific makes Reagent Alcohol with just ethanol and isopropanol. Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epeters2@gmu.edu https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Marcum, Pamela A Sent: Tuesday, January 20, 2015 9:22 AM To: Horn, Hazel V; 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol Hazel is correct if you use the pure ethanol the paperwork is horrible and the limits on what should be in the lab are very low for a normal Histology Lab. ??Reagent alcohol is the best way to go as it has methanol and isopropanol in it so it is undrinkable and therefore not under the same ATF rules of usage. Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Monday, January 19, 2015 1:05 PM To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycling alcohol With Ethyl alcohol you will need a license and will have to keep records. ??With reagent grade alcohol none of that is necessary. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Monday, January 19, 2015 12:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycling alcohol We are planning to recycle alcohol in the new lab I am working with. ?? Previously I always used an alcohol blend such as the "Flex" products. ??However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. ?? We will be getting our alcohol from Thermofisher. ??Can anyone tell me which alcohols they are using for dehydration? ??Reagent alcohol or ethyl alcohol. ??Obviously we will make our own concentrations from a 100%. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois ??62703 Office: ??217-528-7541, Ext. 15121 Email: ??jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 20 Jan 2015 16:51:22 +0000 From: "Marcum, Pamela A" Subject: [Histonet] RE: specials stainers To: "'Jeffrey Robinson'" , "histonet@lists.utsouthwestern.edu" Message-ID: <09c58db6864242c2a9f4371873e68265@MAIL13M2N2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" We use our Leica H&E stainer for PAS only as it is the fastest way we have found to get 30 to 40 bone marrow PAS slides out before 6:30AM every morning. Otherwise it was just too cumbersome and the Dako does a great job on all routine stains. We only do the neuro stains and Copper by hand now. The Dako Copper kit is a different procedure and our pathologist like so it can't be used. The PAS is just too slow for the turnaround we need daily. The stain is great!! Pam Marcum UAMS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Tuesday, January 20, 2015 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: specials stainers I have the Leica multistainer. We use it for H & E staining only. When we attempted to set it up for Special Stains, all of the silver stains had to be made up either each run or each day. Too much hassle. Jeff Robinson, HT, HTL Sierra Pathology Lab, Clovis, CA jrobinson@pathology-associates.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, January 20, 2015 6:13 AM To: 'Mitchell, Janice A'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: specials stainers Oops - I had IHC on the brain and didn't pay close attention! Leica does not have a dedicated special stainer - but they have a multistainer which stains specials as well as H&E. Is too slow in a busy lab tho. I very much recommend the DAKO Artisan.. It is Monday on Tuesday... right? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: Weems, Joyce K. Sent: Tuesday, January 20, 2015 8:59 AM To: 'Mitchell, Janice A'; histonet@lists.utsouthwestern.edu Subject: RE: specials stainers I recommend Leica. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.weems@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 134, Issue 23 ***************************************** PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Tue Jan 20 13:21:02 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Tue Jan 20 13:25:01 2015 Subject: [Histonet] RE: Electronic monitoring systems In-Reply-To: References: Message-ID: <761E2B5697F795489C8710BCC72141FF367ED862@ex07.net.ucsf.edu> We use Sensaphone for our older VIP5 tissue processors, Leica online system for the Peloris processors and Awarepoint for refrigerators/freezers. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Ann Jones Sent: Tuesday, January 20, 2015 11:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Electronic monitoring systems Hi all, I was wondering - a little while ago there was mention on the blog of equipment (processors) being monitored and alerting the 'manager' if they went down. I'm sorry I cannot remember what system this was. What do you all use? Also, what temperature control monitor is everyone using? Ours has flaws and we are gathering information. Can the two systems be combined under one company? Any help is appreciated. Thank you! Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Tue Jan 20 13:29:55 2015 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Jan 20 13:30:05 2015 Subject: [Histonet] RE: Electronic monitoring systems In-Reply-To: References: Message-ID: <25A4DE08332B19499904459F00AAACB719E8D7491D@EVS1.archildrens.org> Our hospital's building automation takes care of this for us. If the tissue processor, freezer, etc. goes into alarm, they call me. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Ann Jones Sent: Tuesday, January 20, 2015 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Electronic monitoring systems Hi all, I was wondering - a little while ago there was mention on the blog of equipment (processors) being monitored and alerting the 'manager' if they went down. I'm sorry I cannot remember what system this was. What do you all use? Also, what temperature control monitor is everyone using? Ours has flaws and we are gathering information. Can the two systems be combined under one company? Any help is appreciated. Thank you! Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From Toni.Rathborne <@t> rwjuh.edu Tue Jan 20 13:38:46 2015 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Tue Jan 20 13:38:53 2015 Subject: [Histonet] VIP 5 Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F2F0D0@vap1014.win.rwjuh.edu> I'm looking for a copy of a User's Manual for our VIP 5. If anyone has one that they could send it would be greatly appreciated! Toni From Toni.Rathborne <@t> rwjuh.edu Tue Jan 20 14:01:39 2015 From: Toni.Rathborne <@t> rwjuh.edu (Rathborne, Toni) Date: Tue Jan 20 14:01:53 2015 Subject: [Histonet] FW: VIP 5 In-Reply-To: <59E09A4EFBD3F349BD75FDAE8AFB0F24F2F0D0@vap1014.win.rwjuh.edu> References: <59E09A4EFBD3F349BD75FDAE8AFB0F24F2F0D0@vap1014.win.rwjuh.edu> Message-ID: <59E09A4EFBD3F349BD75FDAE8AFB0F24F2F11A@vap1014.win.rwjuh.edu> Thank you everyone, I now have the manual. Amazing response time! Toni -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Tuesday, January 20, 2015 2:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] VIP 5 I'm looking for a copy of a User's Manual for our VIP 5. If anyone has one that they could send it would be greatly appreciated! Toni From mjones <@t> metropath.com Tue Jan 20 14:31:51 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Tue Jan 20 14:32:02 2015 Subject: [Histonet] Re: Electronic monitoring systems In-Reply-To: <761E2B5697F795489C8710BCC72141FF367ED862@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367ED862@ex07.net.ucsf.edu> Message-ID: Thank you! Michael Ann On 1/20/15, 12:21 PM, "Morken, Timothy" wrote: >We use Sensaphone for our older VIP5 tissue processors, Leica online >system for the Peloris processors and Awarepoint for >refrigerators/freezers. > >Tim Morken >Supervisor, Histology, Electron Microscopy and Neuromuscular Special >Studies >UC San Francisco Medical Center >San Francisco, CA > >CONFIDENTIALITY NOTICE: This email message, including any attachments, is >for the sole use of the intended recipient(s) and may contain >confidential, proprietary, and/or privileged information protected by >law. If you are not the intended recipient, you may not use, copy, or >distribute this email message or its attachments. If you believe you have >received this email message in error, please contact the sender by reply >email and destroy all copies of the original message. > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael >Ann Jones >Sent: Tuesday, January 20, 2015 11:13 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Electronic monitoring systems > >Hi all, >I was wondering - a little while ago there was mention on the blog of >equipment (processors) being monitored and alerting the 'manager' if they >went down. I'm sorry I cannot remember what system this was. What do you >all use? >Also, what temperature control monitor is everyone using? Ours has flaws >and we are gathering information. >Can the two systems be combined under one company? >Any help is appreciated. > >Thank you! >Michael Ann >Michael Ann Jones, HT (ASCP) >Histology Manager >Metropath >7444 W. Alaska Dr. #250 >Lakewood, CO 80226 >303.634.2511 >Mjones@metropath.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Wed Jan 21 04:33:55 2015 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jan 21 04:34:13 2015 Subject: [Histonet] RE: Electronic monitoring systems In-Reply-To: References: Message-ID: We are tied into the main lab system. Rees Scientific for freezers, refrigerators, and VIPs. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael Ann Jones Sent: Tuesday, January 20, 2015 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Electronic monitoring systems Hi all, I was wondering - a little while ago there was mention on the blog of equipment (processors) being monitored and alerting the 'manager' if they went down. I'm sorry I cannot remember what system this was. What do you all use? Also, what temperature control monitor is everyone using? Ours has flaws and we are gathering information. Can the two systems be combined under one company? Any help is appreciated. Thank you! Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From tmcampbell <@t> fmh.org Wed Jan 21 09:11:59 2015 From: tmcampbell <@t> fmh.org (Campbell, Tasha M.) Date: Wed Jan 21 09:12:02 2015 Subject: [Histonet] embedding Message-ID: <3566D9E34287BE4B95372179009446A02586DB56@EXCHANGE.fmhnt.fmh.org> Hello everyone, I was just curious if anyone had tips on how to embed without getting paraffin on the outside of the cassettes so I don't have to scrape the blocks or at least not scrape very much. Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) From amurvosh <@t> advancederm.net Wed Jan 21 09:27:50 2015 From: amurvosh <@t> advancederm.net (Anne Murvosh) Date: Wed Jan 21 09:28:09 2015 Subject: [Histonet] RE: embedding In-Reply-To: <3566D9E34287BE4B95372179009446A02586DB56@EXCHANGE.fmhnt.fmh.org> References: <3566D9E34287BE4B95372179009446A02586DB56@EXCHANGE.fmhnt.fmh.org> Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7A615C6@Exchange.Advancederm.net> My first job the molds were kept room temp or cool and I didn't have to scrape as much. My 2nd job they were kept hot and had to scrape all the time. Have fun with it and test some cold, some room temp, some hot see if there is a difference. The problem with too cool molds is it takes a bit longer to embed cause the bottom takes longer to warm up so it's a trade off for time. Fun experiment though! Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. Sent: Wednesday, January 21, 2015 7:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding Hello everyone, I was just curious if anyone had tips on how to embed without getting paraffin on the outside of the cassettes so I don't have to scrape the blocks or at least not scrape very much. Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer <@t> mdanderson.org Wed Jan 21 09:32:28 2015 From: TNMayer <@t> mdanderson.org (Mayer,Toysha N) Date: Wed Jan 21 09:33:17 2015 Subject: [Histonet] RE: special stainers Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC881D4BD6EC@D1PWPEXMBX05.mdanderson.edu> It depends on what your conditions are. Dako if users have pretty good special stain knowledge. If for some reason the instrument stops (power outage or something) you can see where the unit stopped and continue the stain by hand. Also, it can be tweaked for your individual needs more. My old pathologist didn't like the PAS off the stainer, so we heated the first special stains wash and that gave him the color difference that was desired. Ventana does not allow such liberties, nor can you use all of the reagent in their vials, so some of it goes to waste. The up side is that if your techs do not have much experience in special stains, the unit can be run by a newbie without much effort if QC'd correctly. Hope this helps. Sincerely, Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A Sent: Tuesday, January 20, 2015 6:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] specials stainers Good Morning, We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts? Thanks for any input, Janice Mitchell _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. From bakevictoria <@t> gmail.com Wed Jan 21 09:40:46 2015 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Wed Jan 21 09:40:51 2015 Subject: [Histonet] RE: embedding In-Reply-To: <22BDD9AABC13E24E95D1CF064B75C4B7A615C6@Exchange.Advancederm.net> References: <3566D9E34287BE4B95372179009446A02586DB56@EXCHANGE.fmhnt.fmh.org> <22BDD9AABC13E24E95D1CF064B75C4B7A615C6@Exchange.Advancederm.net> Message-ID: I have found really hot molds more difficult to work with but it is really an individual preference. What has worked for me is - put just enough paraffin in base mold to come up to rim of embedding area. Embed tissue. Add just a little more paraffin to bring it up to where plastic cassette top comes into contact with hot paraffin. Tilt mold gently to spread paraffin. Press cassette top down and add enough paraffin to cassette top to come up half way. Do 5 - 6 blocks and then top off with hot paraffin. The paraffin has had a chance to harden just enough to where it won't run out from under the cassette and remain pretty clean. Everyone has there tricks you just need to see what works best for you. Vikki On Jan 21, 2015 10:28 AM, "Anne Murvosh" wrote: > My first job the molds were kept room temp or cool and I didn't have to > scrape as much. My 2nd job they were kept hot and had to scrape all the > time. Have fun with it and test some cold, some room temp, some hot see if > there is a difference. The problem with too cool molds is it takes a bit > longer to embed cause the bottom takes longer to warm up so it's a trade > off for time. Fun experiment though! Anne > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. > Sent: Wednesday, January 21, 2015 7:12 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] embedding > > Hello everyone, I was just curious if anyone had tips on how to embed > without getting paraffin on the outside of the cassettes so I don't have to > scrape the blocks or at least not scrape very much. Thanks!! > > > > > > > > > > Tasha Campbell, B.S.,HTL(ASCP) > > Frederick Gastroenterology Associates > > 310 W. 9th St. > > Frederick, MD 21701 > > 301-695-6800 ext. 144 (w) > > 304-685-9307 (c) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PAMarcum <@t> uams.edu Wed Jan 21 10:03:47 2015 From: PAMarcum <@t> uams.edu (Marcum, Pamela A) Date: Wed Jan 21 10:03:55 2015 Subject: [Histonet] Arkansas Society for Histotechnology Spring Meeting and HT Readiness All Day Class Message-ID: <4fda54be1e5f4183a3ab6527937a5529@MAIL13M2N2.ad.uams.edu> Good Morning! We are holding our regular Arkansas Society for Histotechnology Meeting on March 7th, 2015, in Little Rock AR. This will be a one day meeting with eight speakers on topic from the Medical Examiner' Office to general/IHC for the Clinical Histology Laboratory. We are also offering an all-day HT Readiness class with Shane Jones as the presenter. We would love to have any and all come to the meeting and join us for a day of learning and fun. Exhibitors from many major companies will be there for the breaks and the Meet and Greet on Friday evening. If you want further information please contact me at: ashnews@comcast.net and we will send out registration immediately and full programs this weekend or earlier. The cost will $60.00 for the meeting and if you wish to join ASH an additional $20.00 for one year. The all-day HT Readiness will the same price of $60.00 for the day. Lunch and 2 breaks will be provided as well as the Meet and Greet for this fee. Thank You, Pam Marcum ---------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From gayle.callis <@t> bresnan.net Wed Jan 21 10:10:42 2015 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Wed Jan 21 10:11:37 2015 Subject: [Histonet] Re: Embedding Message-ID: <000001d03594$cee09100$6ca1b300$@bresnan.net> After years of never winning the battle of paraffin on cassette edges after embedding, we purchased a paraffin block trimmer. It saves time and the stress on finger joints compared to scraping cassettes daily. No matter how careful we were during embedding to keep excess paraffin off cassette edges, we were never successful. Several vendors have these and you may be able to find a refurbished one. Gayle M. Callis HTL/HT/MT(ASCP) From TGoins <@t> mt.gov Wed Jan 21 10:29:28 2015 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Wed Jan 21 10:32:19 2015 Subject: [Histonet] Re: Embedding In-Reply-To: <000001d03594$cee09100$6ca1b300$@bresnan.net> References: <000001d03594$cee09100$6ca1b300$@bresnan.net> Message-ID: I agree with Gayle. We finally purchased a trimmer from Ted Pella - lowest price by far - and are saving our finger joints. The amount of wax remaining on the cassette also appears to depend on the brand of mold used. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Wednesday, January 21, 2015 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Embedding After years of never winning the battle of paraffin on cassette edges after embedding, we purchased a paraffin block trimmer. It saves time and the stress on finger joints compared to scraping cassettes daily. No matter how careful we were during embedding to keep excess paraffin off cassette edges, we were never successful. Several vendors have these and you may be able to find a refurbished one. Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Jan 21 10:38:33 2015 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Wed Jan 21 10:39:00 2015 Subject: [Histonet] RE: embedding In-Reply-To: <3566D9E34287BE4B95372179009446A02586DB56@EXCHANGE.fmhnt.fmh.org> References: <3566D9E34287BE4B95372179009446A02586DB56@EXCHANGE.fmhnt.fmh.org> Message-ID: <3B2CD438E1628A41BD687E98B963B7812EBB2C6D@EMBX-CLFT4.cdc.gov> Honestly, the easiest thing I think is just don't overfill the base mold. A small amount in the bottom, then the tissue, then the cassette top and just top off enough paraffin to cover. Unless you accidentally slosh it as you are moving it to the cooling tray you will always have clean sides. Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. Sent: Wednesday, January 21, 2015 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding Hello everyone, I was just curious if anyone had tips on how to embed without getting paraffin on the outside of the cassettes so I don't have to scrape the blocks or at least not scrape very much. Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken <@t> ucsf.edu Wed Jan 21 10:41:41 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Wed Jan 21 10:48:44 2015 Subject: [Histonet] Re: Embedding In-Reply-To: References: <000001d03594$cee09100$6ca1b300$@bresnan.net> Message-ID: <761E2B5697F795489C8710BCC72141FF367EDCED@ex07.net.ucsf.edu> I agree. We got one of these a couple years ago and the techs love it. It is a heated block on which you rub the cassette. The paraffin melts away. It is especially good for preserving barcodes (but don't press the printed surface on the heat block too long - you can soften the print and cause some damage, but nothing like can happen with scraping). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, January 21, 2015 8:29 AM To: gayle.callis@bresnan.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Embedding I agree with Gayle. We finally purchased a trimmer from Ted Pella - lowest price by far - and are saving our finger joints. The amount of wax remaining on the cassette also appears to depend on the brand of mold used. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Wednesday, January 21, 2015 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Embedding After years of never winning the battle of paraffin on cassette edges after embedding, we purchased a paraffin block trimmer. It saves time and the stress on finger joints compared to scraping cassettes daily. No matter how careful we were during embedding to keep excess paraffin off cassette edges, we were never successful. Several vendors have these and you may be able to find a refurbished one. Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JRobinson <@t> pathology-associates.com Wed Jan 21 10:57:19 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Wed Jan 21 10:57:42 2015 Subject: [Histonet] RE: embedding In-Reply-To: <3B2CD438E1628A41BD687E98B963B7812EBB2C6D@EMBX-CLFT4.cdc.gov> References: <3566D9E34287BE4B95372179009446A02586DB56@EXCHANGE.fmhnt.fmh.org> <3B2CD438E1628A41BD687E98B963B7812EBB2C6D@EMBX-CLFT4.cdc.gov> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8B0D4@PAEXCH1.PathologyAssociates.local> I have also noticed a big difference in the brand of cassette being used. The old Tissue-Tek style with the large holes do not seem to bleed or leak much at all but the General Data ones we use "bleed" a lot. I have tried different methods with the General Data type but have yet to come up with a sure fire fix. It could also be affected by the brand of mold- I think most of ours are the Tissue-Tek metal ones so they are probably a better "fit" for the Tissue-Tek cassettes. Jeff Robinson, HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, January 21, 2015 8:39 AM To: Campbell, Tasha M.; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: embedding Honestly, the easiest thing I think is just don't overfill the base mold. A small amount in the bottom, then the tissue, then the cassette top and just top off enough paraffin to cover. Unless you accidentally slosh it as you are moving it to the cooling tray you will always have clean sides. Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jqb7@cdc.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. Sent: Wednesday, January 21, 2015 10:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding Hello everyone, I was just curious if anyone had tips on how to embed without getting paraffin on the outside of the cassettes so I don't have to scrape the blocks or at least not scrape very much. Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From tbraud <@t> holyredeemer.com Wed Jan 21 12:16:08 2015 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Jan 21 12:16:24 2015 Subject: [Histonet] Positive ID verification Message-ID: Hi Gang - For those of you who use any type of on demand slide printer with a verification step at the microtome workstation (verifying or printing slides to block by barcode), what system do you use? How long have you used it? What are the pros and cons? Thanks, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From histotech <@t> imagesbyhopper.com Wed Jan 21 12:59:32 2015 From: histotech <@t> imagesbyhopper.com (histotech@imagesbyhopper.com) Date: Wed Jan 21 12:59:49 2015 Subject: [Histonet] Re: Embedding In-Reply-To: <761E2B5697F795489C8710BCC72141FF367EDCED@ex07.net.ucsf.edu> References: <000001d03594$cee09100$6ca1b300$@bresnan.net> <761E2B5697F795489C8710BCC72141FF367EDCED@ex07.net.ucsf.edu> Message-ID: We also use the para-trimmer. In my view, it is worth its weight in gold! I can melt 5 blocks at a time, works like a charm. I am one who does not mind the wax on the sides, as I am most confident that there is enough paraffin to support the cassette. Michelle Sent from my iPhone On Jan 21, 2015, at 11:41 AM, Morken, Timothy wrote: I agree. We got one of these a couple years ago and the techs love it. It is a heated block on which you rub the cassette. The paraffin melts away. It is especially good for preserving barcodes (but don't press the printed surface on the heat block too long - you can soften the print and cause some damage, but nothing like can happen with scraping). Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, January 21, 2015 8:29 AM To: gayle.callis@bresnan.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Embedding I agree with Gayle. We finally purchased a trimmer from Ted Pella - lowest price by far - and are saving our finger joints. The amount of wax remaining on the cassette also appears to depend on the brand of mold used. Tresa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Wednesday, January 21, 2015 9:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Embedding After years of never winning the battle of paraffin on cassette edges after embedding, we purchased a paraffin block trimmer. It saves time and the stress on finger joints compared to scraping cassettes daily. No matter how careful we were during embedding to keep excess paraffin off cassette edges, we were never successful. Several vendors have these and you may be able to find a refurbished one. Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Wed Jan 21 15:44:15 2015 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Wed Jan 21 15:44:21 2015 Subject: [Histonet] Sakura slide racks Message-ID: Histonetters!! I am looking for some replacement racks that hold 20 slides and that fit in the Sakura Tissue-Tek film coverslipper. For some reason our ordering department doesn't want us to/let us order directly from Sakura. Thanks Claire From bcooper <@t> chla.usc.edu Wed Jan 21 15:50:31 2015 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Wed Jan 21 15:50:40 2015 Subject: [Histonet] RE: Sakura slide racks In-Reply-To: References: Message-ID: You can get them from Fisher Scientific. http://www.fishersci.com/ecomm/servlet/itemdetail?itemdetail=%27item%27&storeId=10652&productId=13307075&catalogId=29104&matchedCatNo=NC0046742&fromSearch=1&searchKey=sakura+4768&highlightProductsItemsFlag=Y&endecaSearchQuery=%23store%3DScientific%23nav%3D0%23rpp%3D25%23offSet%3D0%23keyWord%3Dsakura%2B4768%23searchType%3DPROD%23SWKeyList%3D%5B%5D&xrefPartType=From&savings=0.0&xrefEvent=1421876996159_0&searchType=PROD&hasPromo=0 Thanks, Brian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, January 21, 2015 1:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura slide racks Histonetters!! I am looking for some replacement racks that hold 20 slides and that fit in the Sakura Tissue-Tek film coverslipper. For some reason our ordering department doesn't want us to/let us order directly from Sakura. Thanks Claire _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From patrick.lewis <@t> seattlechildrens.org Wed Jan 21 17:52:32 2015 From: patrick.lewis <@t> seattlechildrens.org (Lewis, Patrick) Date: Wed Jan 21 17:52:52 2015 Subject: [Histonet] Cryostat maintenance personnel Message-ID: <3903BE18914F4440834F0E620415FFCA3CB52D52@PPWEXD01d.childrens.sea.kids> Hi everyone, Does anyone know who I would contact about servicing an old CM3000 Cryostat. This would be in the Seattle area and we have no service contract. I am going to call Leica tomorrow, but if anyone has information about repair/servicing that would be helpful. Thanks Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From dabriley <@t> UTMB.EDU Wed Jan 21 20:20:51 2015 From: dabriley <@t> UTMB.EDU (Briley, David) Date: Wed Jan 21 20:21:04 2015 Subject: [Histonet] LCM from tissue on Plus slides Message-ID: Greetings all, I am looking for advice on this issue: I have human tissue samples (hippocampus) which arrived pre-sectioned from a collaborator, affixed to commercial Plus slides. I have been asked to laser-capture microdissect (LCM) a region from these slides. The scientist who taught me LCM suggested that plus slides may not release tissue, but did not have experience with using them in this application herself. ::Can anyone confirm or refute that Plus slides will not allow for efficient retrieval of tissue during LCM? (Arcturus system) ::Does anyone have any recommendations to enhance retrieval of tissue from such slides for the purpose of LCM? Thanks and regards, David Briley University of Texas Medical Branch Galveston Pre-candidacy Doctoral Student From Kathleen.Caleri <@t> RoswellPark.org Thu Jan 22 08:57:13 2015 From: Kathleen.Caleri <@t> RoswellPark.org (Caleri, Kathleen) Date: Thu Jan 22 08:57:20 2015 Subject: [Histonet] cryosectioning biopsies Message-ID: <1B37D44343FCC24BB21204055DF388E59B8DDCEB@EXMB1rsc.roswellpark.org> Hello-In order to preserve scant tissue for future molecular testing, is anyone processing their (needle) biopsies using only frozen sections and subsequently storing the frozen specimens...and NOT paraffin processing them AT ALL? Kate Kathleen Caleri BS HT(ASCP) Histology Lab Supervisor Roswell Park Cancer Institute Elm and Carlton Streets Buffalo, NY 14263 716-845-1329 "There is pleasure in the pathless woods, there is rapture on the lonely shore, There is society where none intrude, by the deep sea and in its roar: I love not Man the less...but Nature more." Lord Byron This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From tony.auge <@t> gmail.com Thu Jan 22 10:58:00 2015 From: tony.auge <@t> gmail.com (Tony Auge) Date: Thu Jan 22 10:58:04 2015 Subject: [Histonet] Re: Embedding In-Reply-To: References: <000001d03594$cee09100$6ca1b300$@bresnan.net> <761E2B5697F795489C8710BCC72141FF367EDCED@ex07.net.ucsf.edu> Message-ID: If you want a cheaper alternative you can use a ski wax iron. They cost about $40. I mounted one upside down in a bucket and it works just as well as the $500 para trimmers. -Tony Auge HTL (ASCP) QIHC From gayle.callis <@t> bresnan.net Thu Jan 22 12:03:40 2015 From: gayle.callis <@t> bresnan.net (Gayle Callis) Date: Thu Jan 22 12:04:47 2015 Subject: [Histonet] Re: Embedding In-Reply-To: References: <000001d03594$cee09100$6ca1b300$@bresnan.net> <761E2B5697F795489C8710BCC72141FF367EDCED@ex07.net.ucsf.edu> Message-ID: <000001d0366d$c150fd70$43f2f850$@bresnan.net> A fabulous idea! I suspect one could use a cheap travel iron although one needs to devise a way to collect melted paraffin. Even our fancy para trimmer didn't have catch pan for paraffin drippings. I suggest using a new or receycled aluminum baking pans available in supermarkets, discount stores or a recycled frozen food pan without separations. These pans come in various sizes and depths. The joy is toss pans when full of paraffin. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Auge Sent: Thursday, January 22, 2015 9:58 AM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Morken, Timothy; Goins, Tresa Subject: Re: [Histonet] Re: Embedding If you want a cheaper alternative you can use a ski wax iron. They cost about $40. I mounted one upside down in a bucket and it works just as well as the $500 para trimmers. -Tony Auge HTL (ASCP) QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From taylor <@t> prometheushealthcare.com Thu Jan 22 12:19:12 2015 From: taylor <@t> prometheushealthcare.com (Taylor Rinaldi) Date: Thu Jan 22 12:14:56 2015 Subject: [Histonet] HT(ascp) or HTL(ascp) job opening in West Philadelphia. Message-ID: <021e01d0366f$ecac9ef0$c605dcd0$@prometheushealthcare.com> Seeking a Histotechnician (HT) or Histotechnologist (HTL) to work in a state-of-the-art laboratory located in suburban Philadelphia. It is a Full time, Permanent opportunity. Monday through Friday, Day shift. ASCP certification required. This position also requires a Bachelor of Science degree in order to perform some high complexity testing. Please reach out to me for immediate consideration. Taylor Rinaldi Taylor Rinaldi Recruiter Prometheus Healthcare Office (301) 693-9057 Taylor@prometheushealthcare.com From DKBoyd <@t> chs.net Thu Jan 22 12:14:00 2015 From: DKBoyd <@t> chs.net (Boyd, Debbie M) Date: Thu Jan 22 12:15:53 2015 Subject: [Histonet] Re: Embedding In-Reply-To: <000001d0366d$c150fd70$43f2f850$@bresnan.net> References: <000001d03594$cee09100$6ca1b300$@bresnan.net> <761E2B5697F795489C8710BCC72141FF367EDCED@ex07.net.ucsf.edu> , <000001d0366d$c150fd70$43f2f850$@bresnan.net> Message-ID: <7EAFE982E328304DA6CE2B677BB76246A9EFBF4C@TN001WEXMBX014.US.chs.net> We use a urine specimen container under the right lower corner of the para trimmer and toss it each day. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Gayle Callis [gayle.callis@bresnan.net] Sent: Thursday, January 22, 2015 1:03 PM To: 'Tony Auge'; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa' Subject: RE: [Histonet] Re: Embedding A fabulous idea! I suspect one could use a cheap travel iron although one needs to devise a way to collect melted paraffin. Even our fancy para trimmer didn't have catch pan for paraffin drippings. I suggest using a new or receycled aluminum baking pans available in supermarkets, discount stores or a recycled frozen food pan without separations. These pans come in various sizes and depths. The joy is toss pans when full of paraffin. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Auge Sent: Thursday, January 22, 2015 9:58 AM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Morken, Timothy; Goins, Tresa Subject: Re: [Histonet] Re: Embedding If you want a cheaper alternative you can use a ski wax iron. They cost about $40. I mounted one upside down in a bucket and it works just as well as the $500 para trimmers. -Tony Auge HTL (ASCP) QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From jshelley <@t> sanfordburnham.org Thu Jan 22 12:35:14 2015 From: jshelley <@t> sanfordburnham.org (John Shelley) Date: Thu Jan 22 12:35:20 2015 Subject: [Histonet] Re: Embedding In-Reply-To: <7EAFE982E328304DA6CE2B677BB76246A9EFBF4C@TN001WEXMBX014.US.chs.net> References: <000001d03594$cee09100$6ca1b300$@bresnan.net> <761E2B5697F795489C8710BCC72141FF367EDCED@ex07.net.ucsf.edu> , <000001d0366d$c150fd70$43f2f850$@bresnan.net> <7EAFE982E328304DA6CE2B677BB76246A9EFBF4C@TN001WEXMBX014.US.chs.net> Message-ID: We just use an empty lid top to the slide boxes. We save them as we load the slide printer, to be used for this and many other tasks. Kind Regards! ? John J Shelley Research Specialist, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road??????????????????????????????? Orlando, FL 32827??????????????????????????????????? Tel: (407) 745-2000 Ext.2517 Lab: (407) 745-2119 Fax: (407) 745-2001 email:? jshelley@sanfordburnham.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Thursday, January 22, 2015 1:14 PM To: gayle.callis@bresnan.net; 'Tony Auge'; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa' Subject: RE: [Histonet] Re: Embedding We use a urine specimen container under the right lower corner of the para trimmer and toss it each day. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Gayle Callis [gayle.callis@bresnan.net] Sent: Thursday, January 22, 2015 1:03 PM To: 'Tony Auge'; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa' Subject: RE: [Histonet] Re: Embedding A fabulous idea! I suspect one could use a cheap travel iron although one needs to devise a way to collect melted paraffin. Even our fancy para trimmer didn't have catch pan for paraffin drippings. I suggest using a new or receycled aluminum baking pans available in supermarkets, discount stores or a recycled frozen food pan without separations. These pans come in various sizes and depths. The joy is toss pans when full of paraffin. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Auge Sent: Thursday, January 22, 2015 9:58 AM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Morken, Timothy; Goins, Tresa Subject: Re: [Histonet] Re: Embedding If you want a cheaper alternative you can use a ski wax iron. They cost about $40. I mounted one upside down in a bucket and it works just as well as the $500 para trimmers. -Tony Auge HTL (ASCP) QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JRobinson <@t> pathology-associates.com Thu Jan 22 12:37:36 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Thu Jan 22 12:38:11 2015 Subject: [Histonet] Re: Embedding In-Reply-To: <7EAFE982E328304DA6CE2B677BB76246A9EFBF4C@TN001WEXMBX014.US.chs.net> References: <000001d03594$cee09100$6ca1b300$@bresnan.net> <761E2B5697F795489C8710BCC72141FF367EDCED@ex07.net.ucsf.edu> , <000001d0366d$c150fd70$43f2f850$@bresnan.net> <7EAFE982E328304DA6CE2B677BB76246A9EFBF4C@TN001WEXMBX014.US.chs.net> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8B1E9@PAEXCH1.PathologyAssociates.local> We use empty pipette tip boxes. They are the perfect size and fit right under the edge and we just toss them when them get full. Jeff Robinson, HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Thursday, January 22, 2015 10:14 AM To: gayle.callis@bresnan.net; 'Tony Auge'; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa' Subject: RE: [Histonet] Re: Embedding We use a urine specimen container under the right lower corner of the para trimmer and toss it each day. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Gayle Callis [gayle.callis@bresnan.net] Sent: Thursday, January 22, 2015 1:03 PM To: 'Tony Auge'; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa' Subject: RE: [Histonet] Re: Embedding A fabulous idea! I suspect one could use a cheap travel iron although one needs to devise a way to collect melted paraffin. Even our fancy para trimmer didn't have catch pan for paraffin drippings. I suggest using a new or receycled aluminum baking pans available in supermarkets, discount stores or a recycled frozen food pan without separations. These pans come in various sizes and depths. The joy is toss pans when full of paraffin. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Auge Sent: Thursday, January 22, 2015 9:58 AM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Morken, Timothy; Goins, Tresa Subject: Re: [Histonet] Re: Embedding If you want a cheaper alternative you can use a ski wax iron. They cost about $40. I mounted one upside down in a bucket and it works just as well as the $500 para trimmers. -Tony Auge HTL (ASCP) QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From TGoins <@t> mt.gov Thu Jan 22 13:02:48 2015 From: TGoins <@t> mt.gov (Goins, Tresa) Date: Thu Jan 22 13:06:40 2015 Subject: [Histonet] Re: Embedding In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D8B1E9@PAEXCH1.PathologyAssociates.local> References: <000001d03594$cee09100$6ca1b300$@bresnan.net> <761E2B5697F795489C8710BCC72141FF367EDCED@ex07.net.ucsf.edu> , <000001d0366d$c150fd70$43f2f850$@bresnan.net> <7EAFE982E328304DA6CE2B677BB76246A9EFBF4C@TN001WEXMBX014.US.chs.net> <204A03EB5A7F0A4BB1EEDD52A963829C16D8B1E9@PAEXCH1.PathologyAssociates.local> Message-ID: Wow! Now I really feel lucky. My "cheap" para trimmer came with a wax catcher with disposable liners that is magnetically attached to the trimmer. http://www.tedpella.com/histo_html/histo1.htm.aspx#_28154 Cant't beat $40 and a practical mind-set though. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Thursday, January 22, 2015 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Embedding We use empty pipette tip boxes. They are the perfect size and fit right under the edge and we just toss them when them get full. Jeff Robinson, HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M Sent: Thursday, January 22, 2015 10:14 AM To: gayle.callis@bresnan.net; 'Tony Auge'; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa' Subject: RE: [Histonet] Re: Embedding We use a urine specimen container under the right lower corner of the para trimmer and toss it each day. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Gayle Callis [gayle.callis@bresnan.net] Sent: Thursday, January 22, 2015 1:03 PM To: 'Tony Auge'; histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; 'Morken, Timothy'; 'Goins, Tresa' Subject: RE: [Histonet] Re: Embedding A fabulous idea! I suspect one could use a cheap travel iron although one needs to devise a way to collect melted paraffin. Even our fancy para trimmer didn't have catch pan for paraffin drippings. I suggest using a new or receycled aluminum baking pans available in supermarkets, discount stores or a recycled frozen food pan without separations. These pans come in various sizes and depths. The joy is toss pans when full of paraffin. Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Auge Sent: Thursday, January 22, 2015 9:58 AM To: histotech@imagesbyhopper.com Cc: histonet@lists.utsouthwestern.edu; Morken, Timothy; Goins, Tresa Subject: Re: [Histonet] Re: Embedding If you want a cheaper alternative you can use a ski wax iron. They cost about $40. I mounted one upside down in a bucket and it works just as well as the $500 para trimmers. -Tony Auge HTL (ASCP) QIHC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From talulahgosh <@t> gmail.com Thu Jan 22 13:14:20 2015 From: talulahgosh <@t> gmail.com (Emily Brown) Date: Thu Jan 22 13:14:28 2015 Subject: [Histonet] Re: Embedding In-Reply-To: References: <000001d03594$cee09100$6ca1b300$@bresnan.net> <761E2B5697F795489C8710BCC72141FF367EDCED@ex07.net.ucsf.edu> Message-ID: That is so cool!! I wish I had one of those!! "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Wed, Jan 21, 2015 at 1:59 PM, histotech@imagesbyhopper.com < histotech@imagesbyhopper.com> wrote: > We also use the para-trimmer. In my view, it is worth its weight in gold! > I can melt 5 blocks at a time, works like a charm. I am one who does not > mind the wax on the sides, as I am most confident that there is enough > paraffin to support the cassette. > > Michelle > > Sent from my iPhone > > On Jan 21, 2015, at 11:41 AM, Morken, Timothy > wrote: > > I agree. We got one of these a couple years ago and the techs love it. It > is a heated block on which you rub the cassette. The paraffin melts away. > It is especially good for preserving barcodes (but don't press the printed > surface on the heat block too long - you can soften the print and cause > some damage, but nothing like can happen with scraping). > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special > Studies > UC San Francisco Medical Center > San Francisco, CA > > CONFIDENTIALITY NOTICE: This email message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential, > proprietary, and/or privileged information protected by law. If you are not > the intended recipient, you may not use, copy, or distribute this email > message or its attachments. If you believe you have received this email > message in error, please contact the sender by reply email and destroy all > copies of the original message. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa > Sent: Wednesday, January 21, 2015 8:29 AM > To: gayle.callis@bresnan.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Embedding > > I agree with Gayle. We finally purchased a trimmer from Ted Pella - > lowest price by far - and are saving our finger joints. The amount of wax > remaining on the cassette also appears to depend on the brand of mold used. > > Tresa > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis > Sent: Wednesday, January 21, 2015 9:11 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Embedding > > After years of never winning the battle of paraffin on cassette edges > after embedding, we purchased a paraffin block trimmer. It saves time and > the stress on finger joints compared to scraping cassettes daily. No > matter how careful we were during embedding to keep excess paraffin off > cassette edges, > we were never successful. Several vendors have these and you may be able > to find a refurbished one. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cfields <@t> mlkch.org Thu Jan 22 13:18:21 2015 From: cfields <@t> mlkch.org (Carol Fields) Date: Thu Jan 22 13:18:28 2015 Subject: [Histonet] FW: Morgue In-Reply-To: References: Message-ID: From: Carol Fields Sent: Thursday, January 22, 2015 11:17 AM To: 'histonet-bounces@lists.utsouthwestern.edu' Subject: Morgue Hi All, Could someone tell me what needs to be posted in the Morgue for inspection please. I know CJD info., this is a new morgue and I'm not sure what all is needed. Thank you in advance. Carole Carole Fields,, HT (ASCP) Lead Histotechnologist, Pathology Laboratory Martin Luther King Jr. Community Hospital 1680 E. 120th Street Los Angeles, CA 90059 O:424-338-8000 x 8306 cfields@mlkch.org www.MLKCommunityHospital.org www.facebook.com/YourMLKCH www.twitter.com/YourMLKCH From craigak12 <@t> gmail.com Thu Jan 22 13:28:33 2015 From: craigak12 <@t> gmail.com (Jb) Date: Thu Jan 22 13:28:41 2015 Subject: [Histonet] Tech Time: Message-ID: I am in need of metrics for the use of requesting an additional histotech. Does anyone have a system in place for presenting the need for additional FTE based on volume, workflow, that you can share? Thank you, Sent from my iPhone From Ryan.Roy <@t> va.gov Thu Jan 22 13:43:44 2015 From: Ryan.Roy <@t> va.gov (Roy, Ryan) Date: Thu Jan 22 13:44:41 2015 Subject: [EXTERNAL] Re: [Histonet] Re: Embedding In-Reply-To: References: <000001d03594$cee09100$6ca1b300$@bresnan.net> <761E2B5697F795489C8710BCC72141FF367EDCED@ex07.net.ucsf.edu> Message-ID: <15F883394EAB744E99E1C7E1B9873049017770029050@R04BYNMSGB1.r04.med.va.gov> I use a block trimmer. I think it is safer for wrists. Being that you push the block against it and I seemed to always pull when I scraped blocks. I am guitar player and need my wrists. I don't think it is a strong argument to say its faster then hand scraping.... atleast not until a good technique is developed by the tech. Ryan Roy HTL (ASCP) Histology Lab Manchester VA 718 Symth Rd Manchester NH 03104 (603) 624-4366 ex 6640 Disclosure: The content of this email does not reflect the policies, views or opions of the VA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Brown Sent: Thursday, January 22, 2015 2:14 PM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Re: Embedding That is so cool!! I wish I had one of those!! "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Wed, Jan 21, 2015 at 1:59 PM, histotech@imagesbyhopper.com < histotech@imagesbyhopper.com> wrote: > We also use the para-trimmer. In my view, it is worth its weight in gold! > I can melt 5 blocks at a time, works like a charm. I am one who does > not mind the wax on the sides, as I am most confident that there is > enough paraffin to support the cassette. > > Michelle > > Sent from my iPhone > > On Jan 21, 2015, at 11:41 AM, Morken, Timothy > > wrote: > > I agree. We got one of these a couple years ago and the techs love it. > It is a heated block on which you rub the cassette. The paraffin melts away. > It is especially good for preserving barcodes (but don't press the > printed surface on the heat block too long - you can soften the print > and cause some damage, but nothing like can happen with scraping). > > Tim Morken > Supervisor, Histology, Electron Microscopy and Neuromuscular Special > Studies UC San Francisco Medical Center San Francisco, CA > > CONFIDENTIALITY NOTICE: This email message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential, proprietary, and/or privileged information protected by > law. If you are not the intended recipient, you may not use, copy, or > distribute this email message or its attachments. If you believe you > have received this email message in error, please contact the sender > by reply email and destroy all copies of the original message. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa > Sent: Wednesday, January 21, 2015 8:29 AM > To: gayle.callis@bresnan.net; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Embedding > > I agree with Gayle. We finally purchased a trimmer from Ted Pella - > lowest price by far - and are saving our finger joints. The amount of > wax remaining on the cassette also appears to depend on the brand of mold used. > > Tresa > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto: > histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis > Sent: Wednesday, January 21, 2015 9:11 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Embedding > > After years of never winning the battle of paraffin on cassette edges > after embedding, we purchased a paraffin block trimmer. It saves > time and the stress on finger joints compared to scraping cassettes > daily. No matter how careful we were during embedding to keep excess > paraffin off cassette edges, > we were never successful. Several vendors have these and you may be able > to find a refurbished one. > > > > Gayle M. Callis > > HTL/HT/MT(ASCP) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wakehealth.edu Thu Jan 22 14:21:53 2015 From: mward <@t> wakehealth.edu (Martha Ward-Pathology) Date: Thu Jan 22 14:21:58 2015 Subject: [Histonet] Her2 IHC validation In-Reply-To: <15F883394EAB744E99E1C7E1B9873049017770029050@R04BYNMSGB1.r04.med.va.gov> References: <000001d03594$cee09100$6ca1b300$@bresnan.net> <761E2B5697F795489C8710BCC72141FF367EDCED@ex07.net.ucsf.edu> <15F883394EAB744E99E1C7E1B9873049017770029050@R04BYNMSGB1.r04.med.va.gov> Message-ID: In advance of preparing for our CAP inspection window, I am working on our validation write-up for our Her2 IHC and was looking for some data concerning the correlation rates. We compared our IHC staining to FISH Her2 results on the same case. Is there a minimum correlation rate? I have been unable to find one in my reading. Thank you in advance for your help. ? Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.2109 ?\? f 336.716.5890 ? mward@wakehealth.edu ? ? ? From wilbanks <@t> caltech.edu Thu Jan 22 14:35:29 2015 From: wilbanks <@t> caltech.edu (Lizzy Wilbanks) Date: Thu Jan 22 14:36:03 2015 Subject: [Histonet] Marker for embedding material? Message-ID: Hi Histonetters, I'm working on embedding bacterial cells in Technovit 8100 and am curious if folks have any recommendations for dyes that can be used as a marker. I first embed my cells in a small agarose plug, then proceed to infiltrate and embed in Technovit 8100. Its possible to see the little agarose plug and flecks of cell material but can be tricky! Does anyone have suggestions for a good dye or marker that I could mix into the agarose so I can better see when trimming and sectioning the block? The only caveat is that it must not be autofluorescent or interfere with *in situ* hybridization we do downstream on these sections. Thanks in advance! Lizzy -- "The obvious goal of any bacterium is to become bacteria." Lizzy Wilbanks Agouron Postdoctoral Fellow, Orphan Lab Division of Geological and Planetary Sciences California Institute of Technology, MC 100-23 Pasadena, CA 91125 From joelleweaver <@t> hotmail.com Thu Jan 22 15:05:25 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Thu Jan 22 15:05:39 2015 Subject: [Histonet] Her2 IHC validation In-Reply-To: References: <000001d03594$cee09100$6ca1b300$@bresnan.net>, , <761E2B5697F795489C8710BCC72141FF367EDCED@ex07.net.ucsf.edu>, , , <15F883394EAB744E99E1C7E1B9873049017770029050@R04BYNMSGB1.r04.med.va.gov>, Message-ID: Yes, check the ASCO/CAP guidelines there are correlation rates for positives and negatives. Joelle Weaver MAOM, HTL (ASCP) QIHC From: mward@wakehealth.edu To: histonet@lists.utsouthwestern.edu Date: Thu, 22 Jan 2015 20:21:53 +0000 Subject: [Histonet] Her2 IHC validation In advance of preparing for our CAP inspection window, I am working on our validation write-up for our Her2 IHC and was looking for some data concerning the correlation rates. We compared our IHC staining to FISH Her2 results on the same case. Is there a minimum correlation rate? I have been unable to find one in my reading. Thank you in advance for your help. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mward@wakehealth.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Jan 22 15:14:45 2015 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Jan 22 15:14:54 2015 Subject: [Histonet] billing question Message-ID: <25A4DE08332B19499904459F00AAACB719E8D74925@EVS1.archildrens.org> Scenario: I am billing for immunostains and there are 3 specimens; A, B, C. Pathologist orders 1 stain on all 3 specimens. I bill 3 88342's. Pathologist orders 2 stains each on A and B. I bill 2 88342's and 2 88341's. Is this correct? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hornhv@archildrens.org archildrens.org ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. From cheastys <@t> svm.vetmed.wisc.edu Thu Jan 22 15:19:46 2015 From: cheastys <@t> svm.vetmed.wisc.edu (Sandra Cheasty) Date: Thu Jan 22 15:19:53 2015 Subject: [Histonet] Cheap Disposable Blades for Facing In Message-ID: <768c5db5a14c684e92b6500bb7293a3b@svm.vetmed.wisc.edu> Hello everyone, Does anyone have a source for cheap, low-profile blades for facing in blocks? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor, President Keith Richards Fan Club UW-Madison, School of Veterinary Medicine From dlschneider <@t> gmail.com Thu Jan 22 15:46:19 2015 From: dlschneider <@t> gmail.com (Daniel Schneider) Date: Thu Jan 22 15:46:23 2015 Subject: [Histonet] billing question In-Reply-To: <25A4DE08332B19499904459F00AAACB719E8D74925@EVS1.archildrens.org> References: <25A4DE08332B19499904459F00AAACB719E8D74925@EVS1.archildrens.org> Message-ID: That's correct, Hazel. Daniel Schneider, MD Amarillo, TX On Thu, Jan 22, 2015 at 3:14 PM, Horn, Hazel V wrote: > Scenario: I am billing for immunostains and there are 3 specimens; A, B, C. > Pathologist orders 1 stain on all 3 specimens. I bill 3 88342's. > Pathologist orders 2 stains each on A and B. I bill 2 88342's and 2 > 88341's. > > Is this correct? > > Hazel Horn > Supervisor of Histology/Autopsy/Transcription > Anatomic Pathology > Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1241 fax > hornhv@archildrens.org > archildrens.org > > > > > > > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and > confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your > computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JMacDonald <@t> mtsac.edu Fri Jan 23 04:59:16 2015 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Jan 23 04:59:23 2015 Subject: [Histonet] Cheap Disposable Blades for Facing In In-Reply-To: <768c5db5a14c684e92b6500bb7293a3b@svm.vetmed.wisc.edu> Message-ID: We save our blade from the previous day to use for facing. We keep them in slide mailers. Sent from my iPad > On Jan 22, 2015, at 4:21 PM, Sandra Cheasty wrote: > > Hello everyone, > > Does anyone have a source for cheap, low-profile blades for facing in blocks? > > Thanks! > Sandy > > > > Sandra J. Cheasty, HT (ASCP) > > Histology & Necropsy Supervisor, President Keith Richards Fan Club > > UW-Madison, School of Veterinary Medicine > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Jan 23 08:29:04 2015 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Jan 23 08:29:16 2015 Subject: [Histonet] CCK testing Message-ID: <5A2BD13465E061429D6455C8D6B40E39173186AB61@IBMB7Exchange.digestivespecialists.com> Dear all, If there is anyone that is doing crystal identification in bile fluid could you contact me off list? Thanks, Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek@digestivespecialists.com From lloyd.3 <@t> osu.edu Wed Jan 21 05:17:02 2015 From: lloyd.3 <@t> osu.edu (Lloyd, Mary) Date: Fri Jan 23 08:32:46 2015 Subject: [Histonet] hpv Message-ID: <322EC297D89B1746938B0BD183E1C7F88A2E117C@CIO-KRC-D1MBX02.osuad.osu.edu> We are having problems finding someone to do HPV staining. Does anyone do HPV staining? From christiecgowan <@t> dermatology.med.ufl.edu Wed Jan 21 10:23:06 2015 From: christiecgowan <@t> dermatology.med.ufl.edu (Gowan,Christie C) Date: Fri Jan 23 08:32:51 2015 Subject: [Histonet] rodent eye In-Reply-To: References: Message-ID: Hi Casie, Hope by now you have rec'd some good tips on rodent eye prep. The only thing I have to offer is that we always used Davidson's fixative for 24 hours and then transferred to 70% ETOH. This worked beautifully preserving all eye components. Good luck and don't forget to check the Histonet archives where I know rodent eyes have been discussed in the past. Christie Gowan HT (ASCP) Department of Dermatology 4037 NW 86th Terrace, 4th Fl Mohs Laboratory Gainesville, FL 32606 Phone: 352 594-1529 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Casie Phillips [casie4384@gmail.com] Sent: Thursday, January 15, 2015 2:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] rodent eye Good afternoon, I am currently working with Lewis rats performing corneal alkali injuries at varying strengths. Is there someone there that has prior experience working with a rat eye and would be willing to share information on the most effective ways to preserve, fix and cut the cornea sample. We are interested in using the cornea without using the whole globe if possible. For now we will be using basic H&E staining with a possibility of immunohistochemistry at a later time. The main outcome we are looking for is to find the presence of neutrophils in the cornea. A second objective is to look for any damaged or newly reconstructed tissue. I would greatly appreciate any advice relating to the type of paraffin used, the ideal length of time to save the tissue and any assistance you can suggest for completing this process successfully. Thank you for your time. Any assistance will be greatly appreciated. Sincerely, Casie Phillips Casie4384@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From twheelock <@t> mclean.harvard.edu Fri Jan 23 08:57:15 2015 From: twheelock <@t> mclean.harvard.edu (Wheelock, Timothy R.) Date: Fri Jan 23 08:57:31 2015 Subject: [Histonet] Cracking paraffin blocks Message-ID: <69718C0B0B3C414D9F8E7214AD400CC9773EABC4@PHSX10MB11.partners.org> Hi Everyone: Recently, I purchased the Medite Valida Embedding Center, which I demoed previously without a problem, if I recall right. I am having problems now with blocks developing cracks on the cold plate. The cracks run either through the wax right next to the specimen, or more frequently, right through the tissue itself. I use Surgipath Embedding Media (EM-400). The surface of the Valida's cold plate is -14C. The company has not seen this happening before, but they are looking into this further. Also, I had the same problem when I demoed the Thermo-Fisher HistoStar. I do not think that this is a problem inherent with any particular machine. Has anyone encountered this problem before? If so, how did you resolve it? Is it possible that the -14C cold plate is too cold? Should I warm it up a bit? The surface of the Valida cold plate is, I believe, made out of smooth stainless steel. My old (24 years) Shandon Embedding Center's cold plate is made out of cast aluminum and has a slightly "rougher" texture. Could this produce a different way of conducting heat out of the paraffin block than the Shandon? Perhaps the stainless steel draws heat from the blocks faster than the aluminum, and thus causes the cracking? I have used the Shandon Embedding Center for over 24 years. I have never had a problem with blocks that crack. Thanks for any thoughts that you may have on this. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From BDeBrosse-Serra <@t> isisph.com Fri Jan 23 09:49:27 2015 From: BDeBrosse-Serra <@t> isisph.com (Bea DeBrosse-Serra) Date: Fri Jan 23 09:49:53 2015 Subject: [Histonet] Cheap Disposable Blades for Facing In In-Reply-To: References: <768c5db5a14c684e92b6500bb7293a3b@svm.vetmed.wisc.edu> Message-ID: We are doing the same. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, January 23, 2015 2:59 AM To: Sandra Cheasty Cc: Histonet (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Cheap Disposable Blades for Facing In We save our blade from the previous day to use for facing. We keep them in slide mailers. Sent from my iPad > On Jan 22, 2015, at 4:21 PM, Sandra Cheasty wrote: > > Hello everyone, > > Does anyone have a source for cheap, low-profile > blades for facing in blocks? > > Thanks! > Sandy > > > > Sandra J. Cheasty, HT (ASCP) > > Histology & Necropsy Supervisor, President Keith Richards Fan Club > > UW-Madison, School of Veterinary Medicine > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hans <@t> histologistics.com Fri Jan 23 11:07:03 2015 From: hans <@t> histologistics.com (Hans B Snyder) Date: Fri Jan 23 11:07:08 2015 Subject: [Histonet] Cracking paraffin blocks In-Reply-To: <69718C0B0B3C414D9F8E7214AD400CC9773EABC4@PHSX10MB11.partners.org> References: <69718C0B0B3C414D9F8E7214AD400CC9773EABC4@PHSX10MB11.partners.org> Message-ID: Hello Tim, I also have had this cracking problem in the past but since resolved. I'm not sure about the specifics of the cracking problem but the cold plate might have something to do with it. Our cold plate is set to -5C and use Paraplast from McCormick Scientific. Our paraffin is roughly the same melting temp as yours, so probably not much difference. Unfortunately trial and error is apart of our jobs since there is relatively little uniformity in histology equipment and products. Have you tried setting the cold plate to a warmer temperature yet? It's worth a try and if that doesn't work maybe the paraffin has been getting too hot. I know when the paraffin gets too hot, the wax and plastic separate or break down and cause inconsistencies in the paraffin. To test this, take an external thermometer and place inside the paraffin tank. Then record the temp every hour for some time. This will tell you if the tank itself is a consistent temperature. Also, are the blocks taken off the cold plate and immediately jarred loose from the mold? Sometimes when I do this the shock of pried out of the mold can cause the paraffin to become brittle and break rather than bend. Let me know how it goes. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Fri, Jan 23, 2015 at 9:57 AM, Wheelock, Timothy R. wrote: > Hi Everyone: > > Recently, I purchased the Medite Valida Embedding Center, which I demoed previously without a problem, if I recall right. > I am having problems now with blocks developing cracks on the cold plate. > The cracks run either through the wax right next to the specimen, or more frequently, right through the tissue itself. > I use Surgipath Embedding Media (EM-400). > The surface of the Valida's cold plate is -14C. > > The company has not seen this happening before, but they are looking into this further. > Also, I had the same problem when I demoed the Thermo-Fisher HistoStar. > I do not think that this is a problem inherent with any particular machine. > Has anyone encountered this problem before? > If so, how did you resolve it? > > Is it possible that the -14C cold plate is too cold? Should I warm it up a bit? > The surface of the Valida cold plate is, I believe, made out of smooth stainless steel. > My old (24 years) Shandon Embedding Center's cold plate is made out of cast aluminum and has a slightly "rougher" texture. > Could this produce a different way of conducting heat out of the paraffin block than the Shandon? > Perhaps the stainless steel draws heat from the blocks faster than the aluminum, and thus causes the cracking? > > I have used the Shandon Embedding Center for over 24 years. > I have never had a problem with blocks that crack. > > Thanks for any thoughts that you may have on this. > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JRobinson <@t> pathology-associates.com Fri Jan 23 11:43:33 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Fri Jan 23 11:43:59 2015 Subject: [Histonet] Amyloid by Congo Red Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8B360@PAEXCH1.PathologyAssociates.local> Greetings to all Histotechs- Here's an amyloid question for the braintrust. We are cutting our slides and controls at 9 and staining in Congo Red for 1 hour. The control stains fine but the patient tissue is staining negative even on cases that the pathologist assures us should be positive for amyloid. We are using the Leica APEX charged slides with control and patient tissue on the same slide. Does anyone have any thoughts on why the patient tissue is not staining? Thanks! Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From cmiller <@t> physlab.com Fri Jan 23 11:54:06 2015 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Jan 23 11:54:13 2015 Subject: [Histonet] Thermo IHC Message-ID: Hi Histonetters! I need some help. We just acquired a Thermo IHC system. Can anyone help us cut through the *****88*888 and give me some helpful tips? We have practical experience on the Ventana systems only. Has anyone been successful at using only 1 HIER buffer? Any tips on how to shorten the offline retrieval process? Currently we are at over 40 mins. Seems to me a ph of 7.6 -7.8 should work for most all of our antibodies. Thanks, Cheri Cheryl A. Miller HT ASCP cm Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 980 2537 Fax 402 731 8653 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. From twheelock <@t> mclean.harvard.edu Fri Jan 23 11:55:12 2015 From: twheelock <@t> mclean.harvard.edu (Wheelock, Timothy R.) Date: Fri Jan 23 11:55:29 2015 Subject: [Histonet] Problem with cracked paraffin blocks Message-ID: <69718C0B0B3C414D9F8E7214AD400CC9773EAC56@PHSX10MB11.partners.org> Hi again everyone: I want to thank you all for your advice. The consensus is that I have my new embedding center's cold plate set way too low at -14C, and that I should try raising it to around -5C to cure my cracked block problem. I will run some test blocks over the weekend, and then embed them at this new temperature on Monday. Otherwise my Valida embedding center is working very nicely (as did the HistoStar when I demoed it). The cassette holding tank can accommodate a large number of brain specimens and the controls are very easy to use. Thanks again for your advice. Have a great weekend. Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From mjones <@t> metropath.com Fri Jan 23 11:58:31 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Fri Jan 23 11:58:37 2015 Subject: [Histonet] Amyloid by Congo Red Message-ID: What protocol and reagents are you using for the stain? Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 1/23/15, 10:43 AM, "Jeffrey Robinson" wrote: >Greetings to all Histotechs- Here's an amyloid question for the >braintrust. We are cutting our slides and controls at 9 and staining in >Congo Red for 1 hour. The control stains fine but the patient tissue is >staining negative even on cases that the pathologist assures us should be >positive for amyloid. We are using the Leica APEX charged slides with >control and patient tissue on the same slide. Does anyone have any >thoughts on why the patient tissue is not staining? Thanks! > >Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, >Clovis, CA. > > >This email and attachments may contain PHI that is privileged and >confidential and is not intended for any unauthorized person. If you, the >reader, are not the intended recipient you are hereby notified that any >dissemination, distribution or copying of this communication is strictly >prohibited. Do not read the email but instead reply to the sender and >destroy the message and any attachments. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From santij1 <@t> bellsouth.net Fri Jan 23 12:12:38 2015 From: santij1 <@t> bellsouth.net (JERRY SANTIAGO) Date: Fri Jan 23 12:12:45 2015 Subject: [Histonet] HT Program Application Message-ID: <1422036758.19423.YahooMailNeo@web180901.mail.ne1.yahoo.com> Hello Everyone, Florida State College at Jacksonville is now accepting applications for the 2015-2016 class of Histologic Technology. This is an online program with scheduled laboratory session at our north campus. For more information, please visit our website at http://www.fscj.edu/academics/areas-of-study/health-human-services/histologic-technology-as or contact Jerry Santiago, Program Director at (904) 766-6528 or jerry.santiago@fscj.edu Please share this information with prospect candidate. From Sarah.Lewis <@t> nationwidechildrens.org Fri Jan 23 12:13:59 2015 From: Sarah.Lewis <@t> nationwidechildrens.org (Lewis, Sarah) Date: Fri Jan 23 12:14:07 2015 Subject: [Histonet] Amyloid by Congo Red In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D8B360@PAEXCH1.PathologyAssociates.local> References: <204A03EB5A7F0A4BB1EEDD52A963829C16D8B360@PAEXCH1.PathologyAssociates.local> Message-ID: <4BAB3E4AF573804EA9929EFD1E15817124FE5A75@RESEXCHMBX03.CRII.ORG> We do all of our Congo Red staining on Muscle and Nerve frozen sections. Sometimes when there is very little amyloid detected using the bright-field/polarizing microscope our pathologist will confirm under a fluorescent microscope with the Rhodamine Red filter. This shows the amyloid inclusions very well. Sarah E. Lewis HT, ASCP The Research Institute at Nationwide Children's Hospital Center for Gene Therapy Neuromuscular Division Rm WA3110 (614)722-2204 -----Original Message----- From: Jeffrey Robinson [mailto:JRobinson@pathology-associates.com] Sent: Friday, January 23, 2015 12:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amyloid by Congo Red Greetings to all Histotechs- Here's an amyloid question for the braintrust. We are cutting our slides and controls at 9 and staining in Congo Red for 1 hour. The control stains fine but the patient tissue is staining negative even on cases that the pathologist assures us should be positive for amyloid. We are using the Leica APEX charged slides with control and patient tissue on the same slide. Does anyone have any thoughts on why the patient tissue is not staining? Thanks! Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From scbymar <@t> yahoo.com Fri Jan 23 12:43:16 2015 From: scbymar <@t> yahoo.com (Maryann Morissette) Date: Fri Jan 23 12:43:24 2015 Subject: [Histonet] HTL exam Message-ID: <55AB30B4-985C-4022-ACB5-02892C34E26B@yahoo.com> Hi all. Was just wondering if anyone has just taken the HTL exam. I passed the HT with just reading an older Frieda Carson book. Can someone give me some advice on books that really helped them? Thanks! Sent from my iPhone > On Jan 23, 2015, at 1:01 PM, histonet-request@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: rodent eye (Gowan,Christie C) > 2. Cracking paraffin blocks (Wheelock, Timothy R.) > 3. RE: Cheap Disposable Blades for Facing In (Bea DeBrosse-Serra) > 4. Re: Cracking paraffin blocks (Hans B Snyder) > 5. Amyloid by Congo Red (Jeffrey Robinson) > 6. Thermo IHC (Cheri Miller) > 7. Problem with cracked paraffin blocks (Wheelock, Timothy R.) > 8. Re: Amyloid by Congo Red (Michael Ann Jones) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 21 Jan 2015 16:23:06 +0000 > From: "Gowan,Christie C" > Subject: RE: [Histonet] rodent eye > To: Casie Phillips , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hi Casie, > Hope by now you have rec'd some good tips on rodent eye prep. The only thing I have to offer is that we always used Davidson's fixative for 24 hours and then transferred to 70% ETOH. This worked beautifully preserving all eye components. Good luck and don't forget to check the Histonet archives where I know rodent eyes have been discussed in the past. > > Christie Gowan HT (ASCP) > > Department of Dermatology > 4037 NW 86th Terrace, 4th Fl > Mohs Laboratory > Gainesville, FL 32606 > Phone: 352 594-1529 > > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Casie Phillips [casie4384@gmail.com] > Sent: Thursday, January 15, 2015 2:53 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] rodent eye > > Good afternoon, > > I am currently working with Lewis rats performing corneal alkali injuries > at varying strengths. Is there someone there that has prior experience > working with a rat eye and would be willing to share information on the > most effective ways to preserve, fix and cut the cornea sample. > > We are interested in using the cornea without using the whole globe if > possible. For now we will be using basic H&E staining with a possibility of > immunohistochemistry at a later time. The main outcome we are looking for > is to find the presence of neutrophils in the cornea. A second objective is > to look for any damaged or newly reconstructed tissue. > > I would greatly appreciate any advice relating to the type of paraffin > used, the ideal length of time to save the tissue and any assistance you > can suggest for completing this process successfully. > > Thank you for your time. Any assistance will be greatly appreciated. > > Sincerely, > > Casie Phillips > Casie4384@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Fri, 23 Jan 2015 14:57:15 +0000 > From: "Wheelock, Timothy R." > Subject: [Histonet] Cracking paraffin blocks > To: "'Histonet@lists.utsouthwestern.edu'" > > Message-ID: > <69718C0B0B3C414D9F8E7214AD400CC9773EABC4@PHSX10MB11.partners.org> > Content-Type: text/plain; charset="us-ascii" > > Hi Everyone: > > Recently, I purchased the Medite Valida Embedding Center, which I demoed previously without a problem, if I recall right. > I am having problems now with blocks developing cracks on the cold plate. > The cracks run either through the wax right next to the specimen, or more frequently, right through the tissue itself. > I use Surgipath Embedding Media (EM-400). > The surface of the Valida's cold plate is -14C. > > The company has not seen this happening before, but they are looking into this further. > Also, I had the same problem when I demoed the Thermo-Fisher HistoStar. > I do not think that this is a problem inherent with any particular machine. > Has anyone encountered this problem before? > If so, how did you resolve it? > > Is it possible that the -14C cold plate is too cold? Should I warm it up a bit? > The surface of the Valida cold plate is, I believe, made out of smooth stainless steel. > My old (24 years) Shandon Embedding Center's cold plate is made out of cast aluminum and has a slightly "rougher" texture. > Could this produce a different way of conducting heat out of the paraffin block than the Shandon? > Perhaps the stainless steel draws heat from the blocks faster than the aluminum, and thus causes the cracking? > > I have used the Shandon Embedding Center for over 24 years. > I have never had a problem with blocks that crack. > > Thanks for any thoughts that you may have on this. > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > ------------------------------ > > Message: 3 > Date: Fri, 23 Jan 2015 15:49:27 +0000 > From: Bea DeBrosse-Serra > Subject: RE: [Histonet] Cheap Disposable Blades for Facing In > To: Jennifer MacDonald , Sandra Cheasty > > Cc: "Histonet \(histonet@lists.utsouthwestern.edu\)" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > We are doing the same. > > Beatrice DeBrosse-Serra HT(ASCP)QIHC > Isis Pharmaceuticals > Antisense Drug Discovery > 2855 Gazelle Ct. > Carlsbad, CA 92010 > 760-603-2371 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald > Sent: Friday, January 23, 2015 2:59 AM > To: Sandra Cheasty > Cc: Histonet (histonet@lists.utsouthwestern.edu) > Subject: Re: [Histonet] Cheap Disposable Blades for Facing In > > > We save our blade from the previous day to use for facing. We keep them in slide mailers. > > Sent from my iPad > >>> On Jan 22, 2015, at 4:21 PM, Sandra Cheasty >> wrote: >> >> Hello everyone, >> >> Does anyone have a source for cheap, low-profile >> blades > for facing in blocks? >> >> Thanks! >> Sandy >> >> >> >> Sandra J. Cheasty, HT (ASCP) >> >> Histology & Necropsy Supervisor, President Keith Richards Fan Club >> >> UW-Madison, School of Veterinary Medicine >> >> >> >> >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Fri, 23 Jan 2015 12:07:03 -0500 > From: Hans B Snyder > Subject: Re: [Histonet] Cracking paraffin blocks > To: "Wheelock, Timothy R." > Cc: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset=UTF-8 > > Hello Tim, > > I also have had this cracking problem in the past but since resolved. > I'm not sure about the specifics of the cracking problem but the cold > plate might have something to do with it. Our cold plate is set to > -5C and use Paraplast from McCormick Scientific. Our paraffin is > roughly the same melting temp as yours, so probably not much > difference. > > Unfortunately trial and error is apart of our jobs since there is > relatively little uniformity in histology equipment and products. Have > you tried setting the cold plate to a warmer temperature yet? It's > worth a try and if that doesn't work maybe the paraffin has been > getting too hot. I know when the paraffin gets too hot, the wax and > plastic separate or break down and cause inconsistencies in the > paraffin. To test this, take an external thermometer and place inside > the paraffin tank. Then record the temp every hour for some time. > This will tell you if the tank itself is a consistent temperature. > > Also, are the blocks taken off the cold plate and immediately jarred > loose from the mold? Sometimes when I do this the shock of pried out > of the mold can cause the paraffin to become brittle and break rather > than bend. > > Let me know how it goes. > > Thank you > Hans B Snyder > Histologistics > 60 Prescott Street > Worcester, MA 01605 > 508-308-7800 > hans@histologistics.com > > > On Fri, Jan 23, 2015 at 9:57 AM, Wheelock, Timothy R. > wrote: >> Hi Everyone: >> >> Recently, I purchased the Medite Valida Embedding Center, which I demoed previously without a problem, if I recall right. >> I am having problems now with blocks developing cracks on the cold plate. >> The cracks run either through the wax right next to the specimen, or more frequently, right through the tissue itself. >> I use Surgipath Embedding Media (EM-400). >> The surface of the Valida's cold plate is -14C. >> >> The company has not seen this happening before, but they are looking into this further. >> Also, I had the same problem when I demoed the Thermo-Fisher HistoStar. >> I do not think that this is a problem inherent with any particular machine. >> Has anyone encountered this problem before? >> If so, how did you resolve it? >> >> Is it possible that the -14C cold plate is too cold? Should I warm it up a bit? >> The surface of the Valida cold plate is, I believe, made out of smooth stainless steel. >> My old (24 years) Shandon Embedding Center's cold plate is made out of cast aluminum and has a slightly "rougher" texture. >> Could this produce a different way of conducting heat out of the paraffin block than the Shandon? >> Perhaps the stainless steel draws heat from the blocks faster than the aluminum, and thus causes the cracking? >> >> I have used the Shandon Embedding Center for over 24 years. >> I have never had a problem with blocks that crack. >> >> Thanks for any thoughts that you may have on this. >> >> Tim Wheelock >> Harvard Brain Bank >> McLean Hospital >> Belmont, MA >> >> >> The information in this e-mail is intended only for the person to whom it is >> addressed. If you believe this e-mail was sent to you in error and the e-mail >> contains patient information, please contact the Partners Compliance HelpLine at >> http://www.partners.org/complianceline . If the e-mail was sent to you in error >> but does not contain patient information, please contact the sender and properly >> dispose of the e-mail. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Fri, 23 Jan 2015 17:43:33 +0000 > From: Jeffrey Robinson > Subject: [Histonet] Amyloid by Congo Red > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > <204A03EB5A7F0A4BB1EEDD52A963829C16D8B360@PAEXCH1.PathologyAssociates.local> > > Content-Type: text/plain; charset="us-ascii" > > Greetings to all Histotechs- Here's an amyloid question for the braintrust. We are cutting our slides and controls at 9 and staining in Congo Red for 1 hour. The control stains fine but the patient tissue is staining negative even on cases that the pathologist assures us should be positive for amyloid. We are using the Leica APEX charged slides with control and patient tissue on the same slide. Does anyone have any thoughts on why the patient tissue is not staining? Thanks! > > Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. > > > This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. > > > ------------------------------ > > Message: 6 > Date: Fri, 23 Jan 2015 11:54:06 -0600 > From: Cheri Miller > Subject: [Histonet] Thermo IHC > To: "histonet@lists.utsouthwestern.edu" > > Cc: "histonet-bounces@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonetters! I need some help. We just acquired a Thermo IHC system. Can anyone help us cut through the *****88*888 and give me some helpful tips? We have practical experience on the Ventana systems only. Has anyone been successful at using only 1 HIER buffer? Any tips on how to shorten the offline retrieval process? Currently we are at over 40 mins. Seems to me a ph of 7.6 -7.8 should work for most all of our antibodies. Thanks, Cheri > > Cheryl A. Miller HT ASCP cm > Physicians Laboratory, P.C. > 4840 F St. > Omaha , NE. 68117 > 402 731 4145 ext. 532 > Cell 402 980 2537 > Fax 402 731 8653 > > ________________________________ > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > > > ------------------------------ > > Message: 7 > Date: Fri, 23 Jan 2015 17:55:12 +0000 > From: "Wheelock, Timothy R." > Subject: [Histonet] Problem with cracked paraffin blocks > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <69718C0B0B3C414D9F8E7214AD400CC9773EAC56@PHSX10MB11.partners.org> > Content-Type: text/plain; charset="us-ascii" > > Hi again everyone: > > I want to thank you all for your advice. > The consensus is that I have my new embedding center's cold plate set way too low at -14C, and that I should try raising it to around -5C to cure my cracked block problem. > I will run some test blocks over the weekend, and then embed them at this new temperature on Monday. > Otherwise my Valida embedding center is working very nicely (as did the HistoStar when I demoed it). > The cassette holding tank can accommodate a large number of brain specimens and the controls are very easy to use. > Thanks again for your advice. > Have a great weekend. > > Tim > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > ------------------------------ > > Message: 8 > Date: Fri, 23 Jan 2015 17:58:31 +0000 > From: Michael Ann Jones > Subject: Re: [Histonet] Amyloid by Congo Red > To: Jeffrey Robinson , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > What protocol and reagents are you using for the stain? > Michael Ann Jones, HT (ASCP) > Histology Manager > Metropath > 7444 W. Alaska Dr. #250 > Lakewood, CO 80226 > 303.634.2511 > Mjones@metropath.com > > > > > > > On 1/23/15, 10:43 AM, "Jeffrey Robinson" > wrote: > >> Greetings to all Histotechs- Here's an amyloid question for the >> braintrust. We are cutting our slides and controls at 9 and staining in >> Congo Red for 1 hour. The control stains fine but the patient tissue is >> staining negative even on cases that the pathologist assures us should be >> positive for amyloid. We are using the Leica APEX charged slides with >> control and patient tissue on the same slide. Does anyone have any >> thoughts on why the patient tissue is not staining? Thanks! >> >> Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, >> Clovis, CA. >> >> >> This email and attachments may contain PHI that is privileged and >> confidential and is not intended for any unauthorized person. If you, the >> reader, are not the intended recipient you are hereby notified that any >> dissemination, distribution or copying of this communication is strictly >> prohibited. Do not read the email but instead reply to the sender and >> destroy the message and any attachments. Thank you. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 134, Issue 28 > ***************************************** From JRobinson <@t> pathology-associates.com Fri Jan 23 12:50:28 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Fri Jan 23 12:50:47 2015 Subject: [Histonet] RE: Problem with cracked paraffin blocks In-Reply-To: <69718C0B0B3C414D9F8E7214AD400CC9773EAC56@PHSX10MB11.partners.org> References: <69718C0B0B3C414D9F8E7214AD400CC9773EAC56@PHSX10MB11.partners.org> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8B3D3@PAEXCH1.PathologyAssociates.local> Regarding the paraffin/plastic stratification: we used to get the reddish "paraffin dust bunnies" in the paraffin pots apparently from the separation and stratification of the paraffin and the plasticizers. I bought a big wood paddle from the industrial kitchen supply store (about $12) and we stir the melted paraffin in the paraffin pots once a day to help keep it mixed up. It seems to have helped some. Jeff Robinson, HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Wheelock, Timothy R. Sent: Friday, January 23, 2015 9:55 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Problem with cracked paraffin blocks Hi again everyone: I want to thank you all for your advice. The consensus is that I have my new embedding center's cold plate set way too low at -14C, and that I should try raising it to around -5C to cure my cracked block problem. I will run some test blocks over the weekend, and then embed them at this new temperature on Monday. Otherwise my Valida embedding center is working very nicely (as did the HistoStar when I demoed it). The cassette holding tank can accommodate a large number of brain specimens and the controls are very easy to use. Thanks again for your advice. Have a great weekend. Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. From JRobinson <@t> pathology-associates.com Fri Jan 23 13:08:47 2015 From: JRobinson <@t> pathology-associates.com (Jeffrey Robinson) Date: Fri Jan 23 13:09:09 2015 Subject: [Histonet] HTL exam In-Reply-To: <55AB30B4-985C-4022-ACB5-02892C34E26B@yahoo.com> References: <55AB30B4-985C-4022-ACB5-02892C34E26B@yahoo.com> Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8B3FE@PAEXCH1.PathologyAssociates.local> I used the NSH booklets on the various Histology subjects. I don't know about their current availability- I think they were on a CD now but I haven't checked lately. I learned a lot from the booklets as they not only give the correct answer they also described why the other answers were wrong along with some background pertaining to related subjects. With the test being online now I don't know how important the color plates of the various special stains are but I found it extremely helpful to know all of the stains by sight backwards and forwards- even stains that we did not run in our lab as there were a lot of questions that would refer to different methods of staining for the same structure or organism, etc. I used Sheehan and Bancroft as my texts. Bancroft is British so there is a different slant to his writing that I find interesting. I have read Carson's but I do not feel it has enough background information. Lee Luna's last book has great color plates but the organization is poor and it can be hard to find things- I think someone finished it up after he passed away. Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maryann Morissette Sent: Friday, January 23, 2015 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HTL exam Hi all. Was just wondering if anyone has just taken the HTL exam. I passed the HT with just reading an older Frieda Carson book. Can someone give me some advice on books that really helped them? Thanks! Sent from my iPhone > On Jan 23, 2015, at 1:01 PM, histonet-request@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: rodent eye (Gowan,Christie C) > 2. Cracking paraffin blocks (Wheelock, Timothy R.) > 3. RE: Cheap Disposable Blades for Facing In (Bea DeBrosse-Serra) > 4. Re: Cracking paraffin blocks (Hans B Snyder) > 5. Amyloid by Congo Red (Jeffrey Robinson) > 6. Thermo IHC (Cheri Miller) > 7. Problem with cracked paraffin blocks (Wheelock, Timothy R.) > 8. Re: Amyloid by Congo Red (Michael Ann Jones) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 21 Jan 2015 16:23:06 +0000 > From: "Gowan,Christie C" > Subject: RE: [Histonet] rodent eye > To: Casie Phillips , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hi Casie, > Hope by now you have rec'd some good tips on rodent eye prep. The only thing I have to offer is that we always used Davidson's fixative for 24 hours and then transferred to 70% ETOH. This worked beautifully preserving all eye components. Good luck and don't forget to check the Histonet archives where I know rodent eyes have been discussed in the past. > > Christie Gowan HT (ASCP) > > Department of Dermatology > 4037 NW 86th Terrace, 4th Fl > Mohs Laboratory > Gainesville, FL 32606 > Phone: 352 594-1529 > > > ________________________________________ > From: histonet-bounces@lists.utsouthwestern.edu > [histonet-bounces@lists.utsouthwestern.edu] on behalf of Casie > Phillips [casie4384@gmail.com] > Sent: Thursday, January 15, 2015 2:53 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] rodent eye > > Good afternoon, > > I am currently working with Lewis rats performing corneal alkali > injuries at varying strengths. Is there someone there that has prior > experience working with a rat eye and would be willing to share > information on the most effective ways to preserve, fix and cut the cornea sample. > > We are interested in using the cornea without using the whole globe if > possible. For now we will be using basic H&E staining with a > possibility of immunohistochemistry at a later time. The main outcome > we are looking for is to find the presence of neutrophils in the > cornea. A second objective is to look for any damaged or newly reconstructed tissue. > > I would greatly appreciate any advice relating to the type of paraffin > used, the ideal length of time to save the tissue and any assistance > you can suggest for completing this process successfully. > > Thank you for your time. Any assistance will be greatly appreciated. > > Sincerely, > > Casie Phillips > Casie4384@gmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Fri, 23 Jan 2015 14:57:15 +0000 > From: "Wheelock, Timothy R." > Subject: [Histonet] Cracking paraffin blocks > To: "'Histonet@lists.utsouthwestern.edu'" > > Message-ID: > <69718C0B0B3C414D9F8E7214AD400CC9773EABC4@PHSX10MB11.partners.org> > Content-Type: text/plain; charset="us-ascii" > > Hi Everyone: > > Recently, I purchased the Medite Valida Embedding Center, which I demoed previously without a problem, if I recall right. > I am having problems now with blocks developing cracks on the cold plate. > The cracks run either through the wax right next to the specimen, or more frequently, right through the tissue itself. > I use Surgipath Embedding Media (EM-400). > The surface of the Valida's cold plate is -14C. > > The company has not seen this happening before, but they are looking into this further. > Also, I had the same problem when I demoed the Thermo-Fisher HistoStar. > I do not think that this is a problem inherent with any particular machine. > Has anyone encountered this problem before? > If so, how did you resolve it? > > Is it possible that the -14C cold plate is too cold? Should I warm it up a bit? > The surface of the Valida cold plate is, I believe, made out of smooth stainless steel. > My old (24 years) Shandon Embedding Center's cold plate is made out of cast aluminum and has a slightly "rougher" texture. > Could this produce a different way of conducting heat out of the paraffin block than the Shandon? > Perhaps the stainless steel draws heat from the blocks faster than the aluminum, and thus causes the cracking? > > I have used the Shandon Embedding Center for over 24 years. > I have never had a problem with blocks that crack. > > Thanks for any thoughts that you may have on this. > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > The information in this e-mail is intended only for the person to whom > it is addressed. If you believe this e-mail was sent to you in error > and the e-mail contains patient information, please contact the > Partners Compliance HelpLine at http://www.partners.org/complianceline > . If the e-mail was sent to you in error but does not contain patient > information, please contact the sender and properly dispose of the e-mail. > > > ------------------------------ > > Message: 3 > Date: Fri, 23 Jan 2015 15:49:27 +0000 > From: Bea DeBrosse-Serra > Subject: RE: [Histonet] Cheap Disposable Blades for Facing In > To: Jennifer MacDonald , Sandra Cheasty > > Cc: "Histonet \(histonet@lists.utsouthwestern.edu\)" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > We are doing the same. > > Beatrice DeBrosse-Serra HT(ASCP)QIHC > Isis Pharmaceuticals > Antisense Drug Discovery > 2855 Gazelle Ct. > Carlsbad, CA 92010 > 760-603-2371 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Jennifer MacDonald > Sent: Friday, January 23, 2015 2:59 AM > To: Sandra Cheasty > Cc: Histonet (histonet@lists.utsouthwestern.edu) > Subject: Re: [Histonet] Cheap Disposable Blades for Facing In > > > We save our blade from the previous day to use for facing. We keep them in slide mailers. > > Sent from my iPad > >>> On Jan 22, 2015, at 4:21 PM, Sandra Cheasty >> wrote: >> >> Hello everyone, >> >> Does anyone have a source for cheap, low-profile >> blades > for facing in blocks? >> >> Thanks! >> Sandy >> >> >> >> Sandra J. Cheasty, HT (ASCP) >> >> Histology & Necropsy Supervisor, President Keith Richards Fan Club >> >> UW-Madison, School of Veterinary Medicine >> >> >> >> >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Fri, 23 Jan 2015 12:07:03 -0500 > From: Hans B Snyder > Subject: Re: [Histonet] Cracking paraffin blocks > To: "Wheelock, Timothy R." > Cc: "Histonet@lists.utsouthwestern.edu" > > Message-ID: > > > Content-Type: text/plain; charset=UTF-8 > > Hello Tim, > > I also have had this cracking problem in the past but since resolved. > I'm not sure about the specifics of the cracking problem but the cold > plate might have something to do with it. Our cold plate is set to > -5C and use Paraplast from McCormick Scientific. Our paraffin is > roughly the same melting temp as yours, so probably not much > difference. > > Unfortunately trial and error is apart of our jobs since there is > relatively little uniformity in histology equipment and products. Have > you tried setting the cold plate to a warmer temperature yet? It's > worth a try and if that doesn't work maybe the paraffin has been > getting too hot. I know when the paraffin gets too hot, the wax and > plastic separate or break down and cause inconsistencies in the > paraffin. To test this, take an external thermometer and place inside > the paraffin tank. Then record the temp every hour for some time. > This will tell you if the tank itself is a consistent temperature. > > Also, are the blocks taken off the cold plate and immediately jarred > loose from the mold? Sometimes when I do this the shock of pried out > of the mold can cause the paraffin to become brittle and break rather > than bend. > > Let me know how it goes. > > Thank you > Hans B Snyder > Histologistics > 60 Prescott Street > Worcester, MA 01605 > 508-308-7800 > hans@histologistics.com > > > On Fri, Jan 23, 2015 at 9:57 AM, Wheelock, Timothy R. > wrote: >> Hi Everyone: >> >> Recently, I purchased the Medite Valida Embedding Center, which I demoed previously without a problem, if I recall right. >> I am having problems now with blocks developing cracks on the cold plate. >> The cracks run either through the wax right next to the specimen, or more frequently, right through the tissue itself. >> I use Surgipath Embedding Media (EM-400). >> The surface of the Valida's cold plate is -14C. >> >> The company has not seen this happening before, but they are looking into this further. >> Also, I had the same problem when I demoed the Thermo-Fisher HistoStar. >> I do not think that this is a problem inherent with any particular machine. >> Has anyone encountered this problem before? >> If so, how did you resolve it? >> >> Is it possible that the -14C cold plate is too cold? Should I warm it up a bit? >> The surface of the Valida cold plate is, I believe, made out of smooth stainless steel. >> My old (24 years) Shandon Embedding Center's cold plate is made out of cast aluminum and has a slightly "rougher" texture. >> Could this produce a different way of conducting heat out of the paraffin block than the Shandon? >> Perhaps the stainless steel draws heat from the blocks faster than the aluminum, and thus causes the cracking? >> >> I have used the Shandon Embedding Center for over 24 years. >> I have never had a problem with blocks that crack. >> >> Thanks for any thoughts that you may have on this. >> >> Tim Wheelock >> Harvard Brain Bank >> McLean Hospital >> Belmont, MA >> >> >> The information in this e-mail is intended only for the person to >> whom it is addressed. If you believe this e-mail was sent to you in >> error and the e-mail contains patient information, please contact the >> Partners Compliance HelpLine at >> http://www.partners.org/complianceline . If the e-mail was sent to >> you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Fri, 23 Jan 2015 17:43:33 +0000 > From: Jeffrey Robinson > Subject: [Histonet] Amyloid by Congo Red > To: "histonet@lists.utsouthwestern.edu" > > Message-ID: > > <204A03EB5A7F0A4BB1EEDD52A963829C16D8B360@PAEXCH1.PathologyAssociates. > local> > > Content-Type: text/plain; charset="us-ascii" > > Greetings to all Histotechs- Here's an amyloid question for the braintrust. We are cutting our slides and controls at 9 and staining in Congo Red for 1 hour. The control stains fine but the patient tissue is staining negative even on cases that the pathologist assures us should be positive for amyloid. We are using the Leica APEX charged slides with control and patient tissue on the same slide. Does anyone have any thoughts on why the patient tissue is not staining? Thanks! > > Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. > > > This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. > > > ------------------------------ > > Message: 6 > Date: Fri, 23 Jan 2015 11:54:06 -0600 > From: Cheri Miller > Subject: [Histonet] Thermo IHC > To: "histonet@lists.utsouthwestern.edu" > > Cc: "histonet-bounces@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonetters! I need some help. We just acquired a Thermo IHC > system. Can anyone help us cut through the *****88*888 and give me > some helpful tips? We have practical experience on the Ventana > systems only. Has anyone been successful at using only 1 HIER buffer? > Any tips on how to shorten the offline retrieval process? Currently we > are at over 40 mins. Seems to me a ph of 7.6 -7.8 should work for most > all of our antibodies. Thanks, Cheri > > Cheryl A. Miller HT ASCP cm > Physicians Laboratory, P.C. > 4840 F St. > Omaha , NE. 68117 > 402 731 4145 ext. 532 > Cell 402 980 2537 > Fax 402 731 8653 > > ________________________________ > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > > > ------------------------------ > > Message: 7 > Date: Fri, 23 Jan 2015 17:55:12 +0000 > From: "Wheelock, Timothy R." > Subject: [Histonet] Problem with cracked paraffin blocks > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <69718C0B0B3C414D9F8E7214AD400CC9773EAC56@PHSX10MB11.partners.org> > Content-Type: text/plain; charset="us-ascii" > > Hi again everyone: > > I want to thank you all for your advice. > The consensus is that I have my new embedding center's cold plate set way too low at -14C, and that I should try raising it to around -5C to cure my cracked block problem. > I will run some test blocks over the weekend, and then embed them at this new temperature on Monday. > Otherwise my Valida embedding center is working very nicely (as did the HistoStar when I demoed it). > The cassette holding tank can accommodate a large number of brain specimens and the controls are very easy to use. > Thanks again for your advice. > Have a great weekend. > > Tim > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > The information in this e-mail is intended only for the person to whom > it is addressed. If you believe this e-mail was sent to you in error > and the e-mail contains patient information, please contact the > Partners Compliance HelpLine at http://www.partners.org/complianceline > . If the e-mail was sent to you in error but does not contain patient > information, please contact the sender and properly dispose of the e-mail. > > > ------------------------------ > > Message: 8 > Date: Fri, 23 Jan 2015 17:58:31 +0000 > From: Michael Ann Jones > Subject: Re: [Histonet] Amyloid by Congo Red > To: Jeffrey Robinson , > "histonet@lists.utsouthwestern.edu" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > What protocol and reagents are you using for the stain? > Michael Ann Jones, HT (ASCP) > Histology Manager > Metropath > 7444 W. Alaska Dr. #250 > Lakewood, CO 80226 > 303.634.2511 > Mjones@metropath.com > > > > > > > On 1/23/15, 10:43 AM, "Jeffrey Robinson" > wrote: > >> Greetings to all Histotechs- Here's an amyloid question for the >> braintrust. We are cutting our slides and controls at 9 and staining >> in Congo Red for 1 hour. The control stains fine but the patient >> tissue is staining negative even on cases that the pathologist >> assures us should be positive for amyloid. We are using the Leica >> APEX charged slides with control and patient tissue on the same >> slide. Does anyone have any thoughts on why the patient tissue is not staining? Thanks! >> >> Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology >> Lab, Clovis, CA. >> >> >> This email and attachments may contain PHI that is privileged and >> confidential and is not intended for any unauthorized person. If you, >> the reader, are not the intended recipient you are hereby notified >> that any dissemination, distribution or copying of this communication >> is strictly prohibited. Do not read the email but instead reply to >> the sender and destroy the message and any attachments. Thank you. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 134, Issue 28 > ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From john <@t> imebinc.com Fri Jan 23 13:22:07 2015 From: john <@t> imebinc.com (John O'Brien) Date: Fri Jan 23 13:24:14 2015 Subject: [Histonet] RE: Histonet Digest, Vol 134, Issue 28 Message-ID: <044301d03741$e0c01130$a2403390$@imebinc.com> COLD plate is way to cold. Regards -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 134, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: rodent eye (Gowan,Christie C) 2. Cracking paraffin blocks (Wheelock, Timothy R.) 3. RE: Cheap Disposable Blades for Facing In (Bea DeBrosse-Serra) 4. Re: Cracking paraffin blocks (Hans B Snyder) 5. Amyloid by Congo Red (Jeffrey Robinson) 6. Thermo IHC (Cheri Miller) 7. Problem with cracked paraffin blocks (Wheelock, Timothy R.) 8. Re: Amyloid by Congo Red (Michael Ann Jones) ---------------------------------------------------------------------- Message: 1 Date: Wed, 21 Jan 2015 16:23:06 +0000 From: "Gowan,Christie C" Subject: RE: [Histonet] rodent eye To: Casie Phillips , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Casie, Hope by now you have rec'd some good tips on rodent eye prep. The only thing I have to offer is that we always used Davidson's fixative for 24 hours and then transferred to 70% ETOH. This worked beautifully preserving all eye components. Good luck and don't forget to check the Histonet archives where I know rodent eyes have been discussed in the past. Christie Gowan HT (ASCP) Department of Dermatology 4037 NW 86th Terrace, 4th Fl Mohs Laboratory Gainesville, FL 32606 Phone: 352 594-1529 ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Casie Phillips [casie4384@gmail.com] Sent: Thursday, January 15, 2015 2:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] rodent eye Good afternoon, I am currently working with Lewis rats performing corneal alkali injuries at varying strengths. Is there someone there that has prior experience working with a rat eye and would be willing to share information on the most effective ways to preserve, fix and cut the cornea sample. We are interested in using the cornea without using the whole globe if possible. For now we will be using basic H&E staining with a possibility of immunohistochemistry at a later time. The main outcome we are looking for is to find the presence of neutrophils in the cornea. A second objective is to look for any damaged or newly reconstructed tissue. I would greatly appreciate any advice relating to the type of paraffin used, the ideal length of time to save the tissue and any assistance you can suggest for completing this process successfully. Thank you for your time. Any assistance will be greatly appreciated. Sincerely, Casie Phillips Casie4384@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Fri, 23 Jan 2015 14:57:15 +0000 From: "Wheelock, Timothy R." Subject: [Histonet] Cracking paraffin blocks To: "'Histonet@lists.utsouthwestern.edu'" Message-ID: <69718C0B0B3C414D9F8E7214AD400CC9773EABC4@PHSX10MB11.partners.org> Content-Type: text/plain; charset="us-ascii" Hi Everyone: Recently, I purchased the Medite Valida Embedding Center, which I demoed previously without a problem, if I recall right. I am having problems now with blocks developing cracks on the cold plate. The cracks run either through the wax right next to the specimen, or more frequently, right through the tissue itself. I use Surgipath Embedding Media (EM-400). The surface of the Valida's cold plate is -14C. The company has not seen this happening before, but they are looking into this further. Also, I had the same problem when I demoed the Thermo-Fisher HistoStar. I do not think that this is a problem inherent with any particular machine. Has anyone encountered this problem before? If so, how did you resolve it? Is it possible that the -14C cold plate is too cold? Should I warm it up a bit? The surface of the Valida cold plate is, I believe, made out of smooth stainless steel. My old (24 years) Shandon Embedding Center's cold plate is made out of cast aluminum and has a slightly "rougher" texture. Could this produce a different way of conducting heat out of the paraffin block than the Shandon? Perhaps the stainless steel draws heat from the blocks faster than the aluminum, and thus causes the cracking? I have used the Shandon Embedding Center for over 24 years. I have never had a problem with blocks that crack. Thanks for any thoughts that you may have on this. Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 3 Date: Fri, 23 Jan 2015 15:49:27 +0000 From: Bea DeBrosse-Serra Subject: RE: [Histonet] Cheap Disposable Blades for Facing In To: Jennifer MacDonald , Sandra Cheasty Cc: "Histonet \(histonet@lists.utsouthwestern.edu\)" Message-ID: Content-Type: text/plain; charset="us-ascii" We are doing the same. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Friday, January 23, 2015 2:59 AM To: Sandra Cheasty Cc: Histonet (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Cheap Disposable Blades for Facing In We save our blade from the previous day to use for facing. We keep them in slide mailers. Sent from my iPad > On Jan 22, 2015, at 4:21 PM, Sandra Cheasty wrote: > > Hello everyone, > > Does anyone have a source for cheap, low-profile > blades for facing in blocks? > > Thanks! > Sandy > > > > Sandra J. Cheasty, HT (ASCP) > > Histology & Necropsy Supervisor, President Keith Richards Fan Club > > UW-Madison, School of Veterinary Medicine > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Fri, 23 Jan 2015 12:07:03 -0500 From: Hans B Snyder Subject: Re: [Histonet] Cracking paraffin blocks To: "Wheelock, Timothy R." Cc: "Histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=UTF-8 Hello Tim, I also have had this cracking problem in the past but since resolved. I'm not sure about the specifics of the cracking problem but the cold plate might have something to do with it. Our cold plate is set to -5C and use Paraplast from McCormick Scientific. Our paraffin is roughly the same melting temp as yours, so probably not much difference. Unfortunately trial and error is apart of our jobs since there is relatively little uniformity in histology equipment and products. Have you tried setting the cold plate to a warmer temperature yet? It's worth a try and if that doesn't work maybe the paraffin has been getting too hot. I know when the paraffin gets too hot, the wax and plastic separate or break down and cause inconsistencies in the paraffin. To test this, take an external thermometer and place inside the paraffin tank. Then record the temp every hour for some time. This will tell you if the tank itself is a consistent temperature. Also, are the blocks taken off the cold plate and immediately jarred loose from the mold? Sometimes when I do this the shock of pried out of the mold can cause the paraffin to become brittle and break rather than bend. Let me know how it goes. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 hans@histologistics.com On Fri, Jan 23, 2015 at 9:57 AM, Wheelock, Timothy R. wrote: > Hi Everyone: > > Recently, I purchased the Medite Valida Embedding Center, which I demoed previously without a problem, if I recall right. > I am having problems now with blocks developing cracks on the cold plate. > The cracks run either through the wax right next to the specimen, or more frequently, right through the tissue itself. > I use Surgipath Embedding Media (EM-400). > The surface of the Valida's cold plate is -14C. > > The company has not seen this happening before, but they are looking into this further. > Also, I had the same problem when I demoed the Thermo-Fisher HistoStar. > I do not think that this is a problem inherent with any particular machine. > Has anyone encountered this problem before? > If so, how did you resolve it? > > Is it possible that the -14C cold plate is too cold? Should I warm it up a bit? > The surface of the Valida cold plate is, I believe, made out of smooth stainless steel. > My old (24 years) Shandon Embedding Center's cold plate is made out of cast aluminum and has a slightly "rougher" texture. > Could this produce a different way of conducting heat out of the paraffin block than the Shandon? > Perhaps the stainless steel draws heat from the blocks faster than the aluminum, and thus causes the cracking? > > I have used the Shandon Embedding Center for over 24 years. > I have never had a problem with blocks that crack. > > Thanks for any thoughts that you may have on this. > > Tim Wheelock > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > The information in this e-mail is intended only for the person to whom > it is addressed. If you believe this e-mail was sent to you in error > and the e-mail contains patient information, please contact the > Partners Compliance HelpLine at http://www.partners.org/complianceline > . If the e-mail was sent to you in error but does not contain patient > information, please contact the sender and properly dispose of the e-mail. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 23 Jan 2015 17:43:33 +0000 From: Jeffrey Robinson Subject: [Histonet] Amyloid by Congo Red To: "histonet@lists.utsouthwestern.edu" Message-ID: <204A03EB5A7F0A4BB1EEDD52A963829C16D8B360@PAEXCH1.PathologyAssociates.local> Content-Type: text/plain; charset="us-ascii" Greetings to all Histotechs- Here's an amyloid question for the braintrust. We are cutting our slides and controls at 9 and staining in Congo Red for 1 hour. The control stains fine but the patient tissue is staining negative even on cases that the pathologist assures us should be positive for amyloid. We are using the Leica APEX charged slides with control and patient tissue on the same slide. Does anyone have any thoughts on why the patient tissue is not staining? Thanks! Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ------------------------------ Message: 6 Date: Fri, 23 Jan 2015 11:54:06 -0600 From: Cheri Miller Subject: [Histonet] Thermo IHC To: "histonet@lists.utsouthwestern.edu" Cc: "histonet-bounces@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters! I need some help. We just acquired a Thermo IHC system. Can anyone help us cut through the *****88*888 and give me some helpful tips? We have practical experience on the Ventana systems only. Has anyone been successful at using only 1 HIER buffer? Any tips on how to shorten the offline retrieval process? Currently we are at over 40 mins. Seems to me a ph of 7.6 -7.8 should work for most all of our antibodies. Thanks, Cheri Cheryl A. Miller HT ASCP cm Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 980 2537 Fax 402 731 8653 ________________________________ PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. ------------------------------ Message: 7 Date: Fri, 23 Jan 2015 17:55:12 +0000 From: "Wheelock, Timothy R." Subject: [Histonet] Problem with cracked paraffin blocks To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <69718C0B0B3C414D9F8E7214AD400CC9773EAC56@PHSX10MB11.partners.org> Content-Type: text/plain; charset="us-ascii" Hi again everyone: I want to thank you all for your advice. The consensus is that I have my new embedding center's cold plate set way too low at -14C, and that I should try raising it to around -5C to cure my cracked block problem. I will run some test blocks over the weekend, and then embed them at this new temperature on Monday. Otherwise my Valida embedding center is working very nicely (as did the HistoStar when I demoed it). The cassette holding tank can accommodate a large number of brain specimens and the controls are very easy to use. Thanks again for your advice. Have a great weekend. Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 8 Date: Fri, 23 Jan 2015 17:58:31 +0000 From: Michael Ann Jones Subject: Re: [Histonet] Amyloid by Congo Red To: Jeffrey Robinson , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" What protocol and reagents are you using for the stain? Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 1/23/15, 10:43 AM, "Jeffrey Robinson" wrote: >Greetings to all Histotechs- Here's an amyloid question for the >braintrust. We are cutting our slides and controls at 9 and staining >in Congo Red for 1 hour. The control stains fine but the patient >tissue is staining negative even on cases that the pathologist assures >us should be positive for amyloid. We are using the Leica APEX charged >slides with control and patient tissue on the same slide. Does anyone >have any thoughts on why the patient tissue is not staining? Thanks! > >Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, >Clovis, CA. > > >This email and attachments may contain PHI that is privileged and >confidential and is not intended for any unauthorized person. If you, >the reader, are not the intended recipient you are hereby notified that >any dissemination, distribution or copying of this communication is >strictly prohibited. Do not read the email but instead reply to the >sender and destroy the message and any attachments. Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 134, Issue 28 ***************************************** From twheelock <@t> mclean.harvard.edu Fri Jan 23 14:00:44 2015 From: twheelock <@t> mclean.harvard.edu (Wheelock, Timothy R.) Date: Fri Jan 23 14:01:05 2015 Subject: [Histonet] Paraffin-Plastic Stratification/Congo red problem Message-ID: <69718C0B0B3C414D9F8E7214AD400CC9773EACE4@PHSX10MB11.partners.org> Hi Jeffery: Yes, I notice the same "paraffin dust bunnies", perhaps especially since I use a embedding paraffin that has a fair amount of plastic. Before I embed my brain tissue, I mix the embedding media thoroughly, until the solution is clear. As for your Congo Red problem, I wish that I could help you. I am now experiencing the same problem, except that it affects the positive controls as well. The staining is there, but much fainter than it should be. I usually immerse albumin coated slides in 1% Congo red for 15 minutes, differentiate them with Potassium Hydroxide for 3 dips, counterstain with Harris Hematoxylin for 1 minute, differentiate with acid alcohol for 5-10 dips, immerse in running tap water for 10 minutes, immerse in 95% ethanol for 2 dips, then dehydrate and mount. Tim The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From jvickroy <@t> SpringfieldClinic.com Fri Jan 23 14:01:02 2015 From: jvickroy <@t> SpringfieldClinic.com (Vickroy, James) Date: Fri Jan 23 14:01:12 2015 Subject: [Histonet] Looking for the ideal temperature ranges and humidity levels - histology lab Message-ID: <9B1A1501A800064397369BD8072E6BCA96F6BC@E2K10DB.springfieldclinic.com> We have always measured the temperature and humidity of the histology lab. Someone today asked what the normal ranges for a histology lab were? Any ideas? Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From joelleweaver <@t> hotmail.com Fri Jan 23 14:13:25 2015 From: joelleweaver <@t> hotmail.com (Joelle Weaver) Date: Fri Jan 23 14:13:44 2015 Subject: [Histonet] HTL exam In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D8B3FE@PAEXCH1.PathologyAssociates.local> References: <55AB30B4-985C-4022-ACB5-02892C34E26B@yahoo.com>, <204A03EB5A7F0A4BB1EEDD52A963829C16D8B3FE@PAEXCH1.PathologyAssociates.local> Message-ID: Agree with your points and book recommendations. I do remember some slide ID on mine. Also there are more taxonomy II & III and operations on the HTL, which warrants a slightly different study approach, but completely acheivable. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: JRobinson@pathology-associates.com > To: scbymar@yahoo.com; histonet@lists.utsouthwestern.edu > Date: Fri, 23 Jan 2015 19:08:47 +0000 > Subject: RE: [Histonet] HTL exam > CC: > > I used the NSH booklets on the various Histology subjects. I don't know about their current availability- I think they were on a CD now but I haven't checked lately. I learned a lot from the booklets as they not only give the correct answer they also described why the other answers were wrong along with some background pertaining to related subjects. With the test being online now I don't know how important the color plates of the various special stains are but I found it extremely helpful to know all of the stains by sight backwards and forwards- even stains that we did not run in our lab as there were a lot of questions that would refer to different methods of staining for the same structure or organism, etc. I used Sheehan and Bancroft as my texts. Bancroft is British so there is a different slant to his writing that I find interesting. I have read Carson's but I do not feel it has enough background information. Lee Luna's last book has great color plates but the organization is poor and it can be hard to find things- I think someone finished it up after he passed away. > > Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maryann Morissette > Sent: Friday, January 23, 2015 10:43 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] HTL exam > > > Hi all. Was just wondering if anyone has just taken the HTL exam. I passed the HT with just reading an older Frieda Carson book. Can someone give me some advice on books that really helped them? Thanks! > Sent from my iPhone > > > On Jan 23, 2015, at 1:01 PM, histonet-request@lists.utsouthwestern.edu wrote: > > > > Send Histonet mailing list submissions to > > histonet@lists.utsouthwestern.edu > > > > To subscribe or unsubscribe via the World Wide Web, visit > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > or, via email, send a message with subject or body 'help' to > > histonet-request@lists.utsouthwestern.edu > > > > You can reach the person managing the list at > > histonet-owner@lists.utsouthwestern.edu > > > > When replying, please edit your Subject line so it is more specific > > than "Re: Contents of Histonet digest..." > > > > > > Today's Topics: > > > > 1. RE: rodent eye (Gowan,Christie C) > > 2. Cracking paraffin blocks (Wheelock, Timothy R.) > > 3. RE: Cheap Disposable Blades for Facing In (Bea DeBrosse-Serra) > > 4. Re: Cracking paraffin blocks (Hans B Snyder) > > 5. Amyloid by Congo Red (Jeffrey Robinson) > > 6. Thermo IHC (Cheri Miller) > > 7. Problem with cracked paraffin blocks (Wheelock, Timothy R.) > > 8. Re: Amyloid by Congo Red (Michael Ann Jones) > > > > > > ---------------------------------------------------------------------- > > > > Message: 1 > > Date: Wed, 21 Jan 2015 16:23:06 +0000 > > From: "Gowan,Christie C" > > Subject: RE: [Histonet] rodent eye > > To: Casie Phillips , > > "histonet@lists.utsouthwestern.edu" > > > > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > > > Hi Casie, > > Hope by now you have rec'd some good tips on rodent eye prep. The only thing I have to offer is that we always used Davidson's fixative for 24 hours and then transferred to 70% ETOH. This worked beautifully preserving all eye components. Good luck and don't forget to check the Histonet archives where I know rodent eyes have been discussed in the past. > > > > Christie Gowan HT (ASCP) > > > > Department of Dermatology > > 4037 NW 86th Terrace, 4th Fl > > Mohs Laboratory > > Gainesville, FL 32606 > > Phone: 352 594-1529 > > > > > > ________________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu > > [histonet-bounces@lists.utsouthwestern.edu] on behalf of Casie > > Phillips [casie4384@gmail.com] > > Sent: Thursday, January 15, 2015 2:53 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] rodent eye > > > > Good afternoon, > > > > I am currently working with Lewis rats performing corneal alkali > > injuries at varying strengths. Is there someone there that has prior > > experience working with a rat eye and would be willing to share > > information on the most effective ways to preserve, fix and cut the cornea sample. > > > > We are interested in using the cornea without using the whole globe if > > possible. For now we will be using basic H&E staining with a > > possibility of immunohistochemistry at a later time. The main outcome > > we are looking for is to find the presence of neutrophils in the > > cornea. A second objective is to look for any damaged or newly reconstructed tissue. > > > > I would greatly appreciate any advice relating to the type of paraffin > > used, the ideal length of time to save the tissue and any assistance > > you can suggest for completing this process successfully. > > > > Thank you for your time. Any assistance will be greatly appreciated. > > > > Sincerely, > > > > Casie Phillips > > Casie4384@gmail.com > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > > > Message: 2 > > Date: Fri, 23 Jan 2015 14:57:15 +0000 > > From: "Wheelock, Timothy R." > > Subject: [Histonet] Cracking paraffin blocks > > To: "'Histonet@lists.utsouthwestern.edu'" > > > > Message-ID: > > <69718C0B0B3C414D9F8E7214AD400CC9773EABC4@PHSX10MB11.partners.org> > > Content-Type: text/plain; charset="us-ascii" > > > > Hi Everyone: > > > > Recently, I purchased the Medite Valida Embedding Center, which I demoed previously without a problem, if I recall right. > > I am having problems now with blocks developing cracks on the cold plate. > > The cracks run either through the wax right next to the specimen, or more frequently, right through the tissue itself. > > I use Surgipath Embedding Media (EM-400). > > The surface of the Valida's cold plate is -14C. > > > > The company has not seen this happening before, but they are looking into this further. > > Also, I had the same problem when I demoed the Thermo-Fisher HistoStar. > > I do not think that this is a problem inherent with any particular machine. > > Has anyone encountered this problem before? > > If so, how did you resolve it? > > > > Is it possible that the -14C cold plate is too cold? Should I warm it up a bit? > > The surface of the Valida cold plate is, I believe, made out of smooth stainless steel. > > My old (24 years) Shandon Embedding Center's cold plate is made out of cast aluminum and has a slightly "rougher" texture. > > Could this produce a different way of conducting heat out of the paraffin block than the Shandon? > > Perhaps the stainless steel draws heat from the blocks faster than the aluminum, and thus causes the cracking? > > > > I have used the Shandon Embedding Center for over 24 years. > > I have never had a problem with blocks that crack. > > > > Thanks for any thoughts that you may have on this. > > > > Tim Wheelock > > Harvard Brain Bank > > McLean Hospital > > Belmont, MA > > > > > > The information in this e-mail is intended only for the person to whom > > it is addressed. If you believe this e-mail was sent to you in error > > and the e-mail contains patient information, please contact the > > Partners Compliance HelpLine at http://www.partners.org/complianceline > > . If the e-mail was sent to you in error but does not contain patient > > information, please contact the sender and properly dispose of the e-mail. > > > > > > ------------------------------ > > > > Message: 3 > > Date: Fri, 23 Jan 2015 15:49:27 +0000 > > From: Bea DeBrosse-Serra > > Subject: RE: [Histonet] Cheap Disposable Blades for Facing In > > To: Jennifer MacDonald , Sandra Cheasty > > > > Cc: "Histonet \(histonet@lists.utsouthwestern.edu\)" > > > > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > > > We are doing the same. > > > > Beatrice DeBrosse-Serra HT(ASCP)QIHC > > Isis Pharmaceuticals > > Antisense Drug Discovery > > 2855 Gazelle Ct. > > Carlsbad, CA 92010 > > 760-603-2371 > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > Jennifer MacDonald > > Sent: Friday, January 23, 2015 2:59 AM > > To: Sandra Cheasty > > Cc: Histonet (histonet@lists.utsouthwestern.edu) > > Subject: Re: [Histonet] Cheap Disposable Blades for Facing In > > > > > > We save our blade from the previous day to use for facing. We keep them in slide mailers. > > > > Sent from my iPad > > > >>> On Jan 22, 2015, at 4:21 PM, Sandra Cheasty > >> wrote: > >> > >> Hello everyone, > >> > >> Does anyone have a source for cheap, low-profile > >> blades > > for facing in blocks? > >> > >> Thanks! > >> Sandy > >> > >> > >> > >> Sandra J. Cheasty, HT (ASCP) > >> > >> Histology & Necropsy Supervisor, President Keith Richards Fan Club > >> > >> UW-Madison, School of Veterinary Medicine > >> > >> > >> > >> > >> > >> > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > > > Message: 4 > > Date: Fri, 23 Jan 2015 12:07:03 -0500 > > From: Hans B Snyder > > Subject: Re: [Histonet] Cracking paraffin blocks > > To: "Wheelock, Timothy R." > > Cc: "Histonet@lists.utsouthwestern.edu" > > > > Message-ID: > > > > > > Content-Type: text/plain; charset=UTF-8 > > > > Hello Tim, > > > > I also have had this cracking problem in the past but since resolved. > > I'm not sure about the specifics of the cracking problem but the cold > > plate might have something to do with it. Our cold plate is set to > > -5C and use Paraplast from McCormick Scientific. Our paraffin is > > roughly the same melting temp as yours, so probably not much > > difference. > > > > Unfortunately trial and error is apart of our jobs since there is > > relatively little uniformity in histology equipment and products. Have > > you tried setting the cold plate to a warmer temperature yet? It's > > worth a try and if that doesn't work maybe the paraffin has been > > getting too hot. I know when the paraffin gets too hot, the wax and > > plastic separate or break down and cause inconsistencies in the > > paraffin. To test this, take an external thermometer and place inside > > the paraffin tank. Then record the temp every hour for some time. > > This will tell you if the tank itself is a consistent temperature. > > > > Also, are the blocks taken off the cold plate and immediately jarred > > loose from the mold? Sometimes when I do this the shock of pried out > > of the mold can cause the paraffin to become brittle and break rather > > than bend. > > > > Let me know how it goes. > > > > Thank you > > Hans B Snyder > > Histologistics > > 60 Prescott Street > > Worcester, MA 01605 > > 508-308-7800 > > hans@histologistics.com > > > > > > On Fri, Jan 23, 2015 at 9:57 AM, Wheelock, Timothy R. > > wrote: > >> Hi Everyone: > >> > >> Recently, I purchased the Medite Valida Embedding Center, which I demoed previously without a problem, if I recall right. > >> I am having problems now with blocks developing cracks on the cold plate. > >> The cracks run either through the wax right next to the specimen, or more frequently, right through the tissue itself. > >> I use Surgipath Embedding Media (EM-400). > >> The surface of the Valida's cold plate is -14C. > >> > >> The company has not seen this happening before, but they are looking into this further. > >> Also, I had the same problem when I demoed the Thermo-Fisher HistoStar. > >> I do not think that this is a problem inherent with any particular machine. > >> Has anyone encountered this problem before? > >> If so, how did you resolve it? > >> > >> Is it possible that the -14C cold plate is too cold? Should I warm it up a bit? > >> The surface of the Valida cold plate is, I believe, made out of smooth stainless steel. > >> My old (24 years) Shandon Embedding Center's cold plate is made out of cast aluminum and has a slightly "rougher" texture. > >> Could this produce a different way of conducting heat out of the paraffin block than the Shandon? > >> Perhaps the stainless steel draws heat from the blocks faster than the aluminum, and thus causes the cracking? > >> > >> I have used the Shandon Embedding Center for over 24 years. > >> I have never had a problem with blocks that crack. > >> > >> Thanks for any thoughts that you may have on this. > >> > >> Tim Wheelock > >> Harvard Brain Bank > >> McLean Hospital > >> Belmont, MA > >> > >> > >> The information in this e-mail is intended only for the person to > >> whom it is addressed. If you believe this e-mail was sent to you in > >> error and the e-mail contains patient information, please contact the > >> Partners Compliance HelpLine at > >> http://www.partners.org/complianceline . If the e-mail was sent to > >> you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > > > Message: 5 > > Date: Fri, 23 Jan 2015 17:43:33 +0000 > > From: Jeffrey Robinson > > Subject: [Histonet] Amyloid by Congo Red > > To: "histonet@lists.utsouthwestern.edu" > > > > Message-ID: > > > > <204A03EB5A7F0A4BB1EEDD52A963829C16D8B360@PAEXCH1.PathologyAssociates. > > local> > > > > Content-Type: text/plain; charset="us-ascii" > > > > Greetings to all Histotechs- Here's an amyloid question for the braintrust. We are cutting our slides and controls at 9 and staining in Congo Red for 1 hour. The control stains fine but the patient tissue is staining negative even on cases that the pathologist assures us should be positive for amyloid. We are using the Leica APEX charged slides with control and patient tissue on the same slide. Does anyone have any thoughts on why the patient tissue is not staining? Thanks! > > > > Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. > > > > > > This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. > > > > > > ------------------------------ > > > > Message: 6 > > Date: Fri, 23 Jan 2015 11:54:06 -0600 > > From: Cheri Miller > > Subject: [Histonet] Thermo IHC > > To: "histonet@lists.utsouthwestern.edu" > > > > Cc: "histonet-bounces@lists.utsouthwestern.edu" > > > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > > > Hi Histonetters! I need some help. We just acquired a Thermo IHC > > system. Can anyone help us cut through the *****88*888 and give me > > some helpful tips? We have practical experience on the Ventana > > systems only. Has anyone been successful at using only 1 HIER buffer? > > Any tips on how to shorten the offline retrieval process? Currently we > > are at over 40 mins. Seems to me a ph of 7.6 -7.8 should work for most > > all of our antibodies. Thanks, Cheri > > > > Cheryl A. Miller HT ASCP cm > > Physicians Laboratory, P.C. > > 4840 F St. > > Omaha , NE. 68117 > > 402 731 4145 ext. 532 > > Cell 402 980 2537 > > Fax 402 731 8653 > > > > ________________________________ > > PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are hereby notified that you are in possession of confidential and privileged information. Any dissemination, distribution, or copying of this e-mail is strictly prohibited. If you have received this message in error, please notify the sender immediately and delete this email from your system. > > > > > > ------------------------------ > > > > Message: 7 > > Date: Fri, 23 Jan 2015 17:55:12 +0000 > > From: "Wheelock, Timothy R." > > Subject: [Histonet] Problem with cracked paraffin blocks > > To: "'histonet@lists.utsouthwestern.edu'" > > > > Message-ID: > > <69718C0B0B3C414D9F8E7214AD400CC9773EAC56@PHSX10MB11.partners.org> > > Content-Type: text/plain; charset="us-ascii" > > > > Hi again everyone: > > > > I want to thank you all for your advice. > > The consensus is that I have my new embedding center's cold plate set way too low at -14C, and that I should try raising it to around -5C to cure my cracked block problem. > > I will run some test blocks over the weekend, and then embed them at this new temperature on Monday. > > Otherwise my Valida embedding center is working very nicely (as did the HistoStar when I demoed it). > > The cassette holding tank can accommodate a large number of brain specimens and the controls are very easy to use. > > Thanks again for your advice. > > Have a great weekend. > > > > Tim > > > > Tim Wheelock > > Harvard Brain Bank > > McLean Hospital > > Belmont, MA > > > > > > The information in this e-mail is intended only for the person to whom > > it is addressed. If you believe this e-mail was sent to you in error > > and the e-mail contains patient information, please contact the > > Partners Compliance HelpLine at http://www.partners.org/complianceline > > . If the e-mail was sent to you in error but does not contain patient > > information, please contact the sender and properly dispose of the e-mail. > > > > > > ------------------------------ > > > > Message: 8 > > Date: Fri, 23 Jan 2015 17:58:31 +0000 > > From: Michael Ann Jones > > Subject: Re: [Histonet] Amyloid by Congo Red > > To: Jeffrey Robinson , > > "histonet@lists.utsouthwestern.edu" > > > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > > What protocol and reagents are you using for the stain? > > Michael Ann Jones, HT (ASCP) > > Histology Manager > > Metropath > > 7444 W. Alaska Dr. #250 > > Lakewood, CO 80226 > > 303.634.2511 > > Mjones@metropath.com > > > > > > > > > > > > > > On 1/23/15, 10:43 AM, "Jeffrey Robinson" > > wrote: > > > >> Greetings to all Histotechs- Here's an amyloid question for the > >> braintrust. We are cutting our slides and controls at 9 and staining > >> in Congo Red for 1 hour. The control stains fine but the patient > >> tissue is staining negative even on cases that the pathologist > >> assures us should be positive for amyloid. We are using the Leica > >> APEX charged slides with control and patient tissue on the same > >> slide. Does anyone have any thoughts on why the patient tissue is not staining? Thanks! > >> > >> Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology > >> Lab, Clovis, CA. > >> > >> > >> This email and attachments may contain PHI that is privileged and > >> confidential and is not intended for any unauthorized person. If you, > >> the reader, are not the intended recipient you are hereby notified > >> that any dissemination, distribution or copying of this communication > >> is strictly prohibited. Do not read the email but instead reply to > >> the sender and destroy the message and any attachments. Thank you. > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > ------------------------------ > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > End of Histonet Digest, Vol 134, Issue 28 > > ***************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Fri Jan 23 14:16:13 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Fri Jan 23 14:16:51 2015 Subject: [Histonet] Looking for the ideal temperature ranges and humidity levels - histology lab In-Reply-To: <9B1A1501A800064397369BD8072E6BCA96F6BC@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA96F6BC@E2K10DB.springfieldclinic.com> Message-ID: We base our ranges on the equipment specifications. That would be the requirement for the rooms. 30 - 80% humidity for most equipment and the room temp (see equipment manuals) Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 1/23/15, 1:01 PM, "Vickroy, James" wrote: > >We have always measured the temperature and humidity of the histology >lab. Someone today asked what the normal ranges for a histology lab >were? Any ideas? > >Jim Vickroy >Histology Manager >Springfield Clinic, Main Campus, East Building >1025 South 6th Street >Springfield, Illinois 62703 >Office: 217-528-7541, Ext. 15121 >Email: >jvickroy@SpringfieldClinic.com > > >This electronic message contains information from Springfield Clinic, LLP >that may be confidential, privileged, and/or sensitive. This information >is intended for the use of the individual(s) or entity(ies) named above. >If you are not the intended recipient, be aware that disclosure, copying, >distribution, or action taken on the contents of this information is >strictly prohibited. If you have received this electronic message in >error, please notify the sender immediately, by electronic mail, so that >arrangements may be made for the retrieval of this electronic message. >Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Fri Jan 23 14:16:30 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Fri Jan 23 14:16:56 2015 Subject: [Histonet] Looking for the ideal temperature ranges and humidity levels - histology lab In-Reply-To: <9B1A1501A800064397369BD8072E6BCA96F6BC@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA96F6BC@E2K10DB.springfieldclinic.com> Message-ID: I forgot - reagent temps too! Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 1/23/15, 1:01 PM, "Vickroy, James" wrote: > >We have always measured the temperature and humidity of the histology >lab. Someone today asked what the normal ranges for a histology lab >were? Any ideas? > >Jim Vickroy >Histology Manager >Springfield Clinic, Main Campus, East Building >1025 South 6th Street >Springfield, Illinois 62703 >Office: 217-528-7541, Ext. 15121 >Email: >jvickroy@SpringfieldClinic.com > > >This electronic message contains information from Springfield Clinic, LLP >that may be confidential, privileged, and/or sensitive. This information >is intended for the use of the individual(s) or entity(ies) named above. >If you are not the intended recipient, be aware that disclosure, copying, >distribution, or action taken on the contents of this information is >strictly prohibited. If you have received this electronic message in >error, please notify the sender immediately, by electronic mail, so that >arrangements may be made for the retrieval of this electronic message. >Thank you. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Fri Jan 23 14:17:49 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Fri Jan 23 14:18:24 2015 Subject: [Histonet] Paraffin-Plastic Stratification/Congo red problem Message-ID: I also noticed stain fading with time - controls cut way ahead of staining? Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 Mjones@metropath.com On 1/23/15, 1:00 PM, "Wheelock, Timothy R." wrote: >Hi Jeffery: > >Yes, I notice the same "paraffin dust bunnies", perhaps especially since >I use a embedding paraffin that has a fair amount of plastic. >Before I embed my brain tissue, I mix the embedding media thoroughly, >until the solution is clear. > >As for your Congo Red problem, I wish that I could help you. >I am now experiencing the same problem, except that it affects the >positive controls as well. >The staining is there, but much fainter than it should be. > >I usually immerse albumin coated slides in 1% Congo red for 15 minutes, >differentiate them with Potassium Hydroxide for 3 dips, counterstain with >Harris Hematoxylin for 1 minute, differentiate with acid alcohol for 5-10 >dips, immerse in running tap water for 10 minutes, immerse in 95% ethanol >for 2 dips, then dehydrate and mount. > >Tim > > > > >The information in this e-mail is intended only for the person to whom it >is >addressed. If you believe this e-mail was sent to you in error and the >e-mail >contains patient information, please contact the Partners Compliance >HelpLine at >http://www.partners.org/complianceline . If the e-mail was sent to you in >error >but does not contain patient information, please contact the sender and >properly >dispose of the e-mail. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Jan 23 14:23:21 2015 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Jan 23 14:23:39 2015 Subject: [Histonet] Looking for the ideal temperature ranges and humidity levels - histology lab In-Reply-To: <9B1A1501A800064397369BD8072E6BCA96F6BC@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA96F6BC@E2K10DB.springfieldclinic.com> Message-ID: <14E2C6176416974295479C64A11CB9AE019C79ECE005@SBS2K8.premierlab.local> Jim Its based upon the equipment requirements from each operating manual, we use the most critical instrument in the room and what the temp and humidity requirements are for that piece of equipment, we find those are usually the most stringent and the requirements for the other equipment in the room usually fall in that range. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Friday, January 23, 2015 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for the ideal temperature ranges and humidity levels - histology lab We have always measured the temperature and humidity of the histology lab. Someone today asked what the normal ranges for a histology lab were? Any ideas? Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy@SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles <@t> uwhealth.org Fri Jan 23 18:30:13 2015 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri Jan 23 18:30:19 2015 Subject: [Histonet] RE: Paraffin-Plastic Stratification/Congo red problem In-Reply-To: <69718C0B0B3C414D9F8E7214AD400CC9773EACE4@PHSX10MB11.partners.org> References: <69718C0B0B3C414D9F8E7214AD400CC9773EACE4@PHSX10MB11.partners.org> Message-ID: I believe amyloid does fade with age on cut slides. Frieda sited Bancroft and Cook that intensity decreases with age and "long-standing" deposits stain less intensely than smaller "Newly formed" deposits. She also lists a Crystal Violet method in the 3rd edition, but that it is more of a rapid screening technique and is not as specific as Congo Red. Claire ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Wheelock, Timothy R. [twheelock@mclean.harvard.edu] Sent: Friday, January 23, 2015 2:00 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Paraffin-Plastic Stratification/Congo red problem Hi Jeffery: Yes, I notice the same "paraffin dust bunnies", perhaps especially since I use a embedding paraffin that has a fair amount of plastic. Before I embed my brain tissue, I mix the embedding media thoroughly, until the solution is clear. As for your Congo Red problem, I wish that I could help you. I am now experiencing the same problem, except that it affects the positive controls as well. The staining is there, but much fainter than it should be. I usually immerse albumin coated slides in 1% Congo red for 15 minutes, differentiate them with Potassium Hydroxide for 3 dips, counterstain with Harris Hematoxylin for 1 minute, differentiate with acid alcohol for 5-10 dips, immerse in running tap water for 10 minutes, immerse in 95% ethanol for 2 dips, then dehydrate and mount. Tim The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From llewllew <@t> shaw.ca Sat Jan 24 15:28:45 2015 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Sat Jan 24 15:29:07 2015 Subject: [Histonet] Amyloid by Congo Red In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D8B360@PAEXCH1.PathologyAssociates.local> References: <204A03EB5A7F0A4BB1EEDD52A963829C16D8B360@PAEXCH1.PathologyAssociates.local> Message-ID: <54C40E8D.1060103@shaw.ca> This is a well known phenomenon of amyloid staining and it is fairly common. It is due to the fact that amyloid is a very variable material and different samples may react differently to the dyes. I suggest you restain using Highman's congo red method (http://stainsfile.info/StainsFile/stain/amyloid/congohighman.htm) is more reliable than Bennhold's. I always found it the more reliable. If it is still unstained, try a sirius red method (using CI 35780, sirius red F3B, NOT sirius red 4B). You might also want to consider thioflavine T and the other methods using dyes other than direct cotton dyes. Read the article, which includes a list of methods, at: http://stainsfile.info/StainsFile/stain/amyloid/amyloid.htm Bryan Llewellyn Jeffrey Robinson wrote: > Greetings to all Histotechs- Here's an amyloid question for the braintrust. We are cutting our slides and controls at 9 and staining in Congo Red for 1 hour. The control stains fine but the patient tissue is staining negative even on cases that the pathologist assures us should be positive for amyloid. We are using the Leica APEX charged slides with control and patient tissue on the same slide. Does anyone have any thoughts on why the patient tissue is not staining? Thanks! > > Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. > > > This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kdwyer3322 <@t> aol.com Sun Jan 25 10:29:41 2015 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sun Jan 25 10:29:47 2015 Subject: [Histonet] Texas Society for Histotechnology Symposium Convention March 20-22, 2015 Message-ID: <8D206D341121F8E-1878-49105@webmail-vm014.sysops.aol.com> Hi Histonetters! The Texas Society for Histotechnology will be holding the 2015 S/C in Plano Texas. If you are interested in a electronic version of the program please send me a e-mail via this communication. We would love to have you join us this year! Regards, Kathy Dwyer "Texas Histology: A Wealth of Knowledge" Texas Society for Histotechnology 37th Annual Symposium/Convention Dallas/Plano Marriott at Legacy TownCenter Plano, Texas March 20-22, 2015 From PREISZNE <@t> mail.etsu.edu Mon Jan 26 08:32:11 2015 From: PREISZNE <@t> mail.etsu.edu (Preiszner, Johanna) Date: Mon Jan 26 08:32:31 2015 Subject: [Histonet] RE: Amyloid by Congo Red In-Reply-To: <204A03EB5A7F0A4BB1EEDD52A963829C16D8B48E@PAEXCH1.PathologyAssociates.local> References: <204A03EB5A7F0A4BB1EEDD52A963829C16D8B360@PAEXCH1.PathologyAssociates.local> , <204A03EB5A7F0A4BB1EEDD52A963829C16D8B48E@PAEXCH1.PathologyAssociates.local> Message-ID: Hi, I can send amyloid positive control tissue block (cerebral amyloid vasculitis), just send me an email to preiszne@etsu.edu with your mailing address. Very nice staining, tissue was harvested two month ago. We have more than we'll ever use up. Cheers, Hanna ________________________________________ From: Jeffrey Robinson [JRobinson@pathology-associates.com] Sent: Friday, January 23, 2015 3:33 PM To: Preiszner, Johanna Subject: RE: Amyloid by Congo Red HI Hanna- you are the second tech suggesting the Sigma kit so I may try that. I have an old Leitz fluorescent scope that is still around somewhere that I used when we did kidney bx's but we are in a new building now without a fluorescent room so I don't think I can set it up. Thanks! Jeff. -----Original Message----- From: Preiszner, Johanna [mailto:PREISZNE@mail.etsu.edu] Sent: Friday, January 23, 2015 12:27 PM To: Jeffrey Robinson Subject: RE: Amyloid by Congo Red Hi, we use the Sigma Kit, and never a had problem. Protocol is different than what you describe so you may want to try it. Also what Sarah mentioned: might need to check the stain on a fluorescence microscope with excitation at 497nm. The emitted signal is very red and we never had non-specific staining. I can send pictures if interested. Hanna Preiszner ETSU/QCOM ________________________________________ From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] on behalf of Jeffrey Robinson [JRobinson@pathology-associates.com] Sent: Friday, January 23, 2015 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amyloid by Congo Red Greetings to all Histotechs- Here's an amyloid question for the braintrust. We are cutting our slides and controls at 9 and staining in Congo Red for 1 hour. The control stains fine but the patient tissue is staining negative even on cases that the pathologist assures us should be positive for amyloid. We are using the Leica APEX charged slides with control and patient tissue on the same slide. Does anyone have any thoughts on why the patient tissue is not staining? Thanks! Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JLongo <@t> peacehealthlabs.org Fri Jan 23 14:00:44 2015 From: JLongo <@t> peacehealthlabs.org (Longo, Julia C (MD)) Date: Mon Jan 26 10:30:33 2015 Subject: [Histonet] New cryostat decontamination CAP statndard Message-ID: Jeff Silverman, I saw your notes online regarding decontaminating cryostats. I was wondering if there is anything new? We have old cryostats that have to be thawed to be decontaminated. We are having a hard time getting this done on both of them every week. Thanks, Julia Longo, MD This message is intended solely for the use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential, and exempt from disclosure under applicable state and federal laws. If you are not the addressee, or are not authorized to receive for the intended addressee, you are hereby notified that you may not use, copy, distribute, or disclose to anyone this message or the information contained herein. If you have received this message in error, immediately advise the sender by reply email and destroy this message. From Lacie.Algeo <@t> providence.org Mon Jan 26 12:46:43 2015 From: Lacie.Algeo <@t> providence.org (Algeo, Lacie A) Date: Mon Jan 26 12:47:06 2015 Subject: [Histonet] temporal arteries Message-ID: <24C4B3C167E5694887AB594C7602CE3A03B8406B@WN35104.or.providence.org> Hi All! Does anyone know of a standard that indicates how many cross sections must be evaluated on a temp art? Also, any standard on running an elastic stain routinely? Currently, we are cutting our temp arts into 8 or so pieces per cassette. After that we do 6 slides with serial sections. So, we're looking at approximately 240 cross sections. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From rjbuesa <@t> yahoo.com Mon Jan 26 15:23:51 2015 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jan 26 15:24:02 2015 Subject: [Histonet] temporal arteries In-Reply-To: <24C4B3C167E5694887AB594C7602CE3A03B8406B@WN35104.or.providence.org> References: <24C4B3C167E5694887AB594C7602CE3A03B8406B@WN35104.or.providence.org> Message-ID: <1450715820.490812.1422307431439.JavaMail.yahoo@mail.yahoo.com> There are no standards about this issue and all will depend on what your pathologist wants to screen.Remember that SUPPOSEDLY your PT will have to screen avery thing you?do.Ask your PT in what is s/he wants to screen to feel "comfortable" with the diagnosis.Ren? J.? On Monday, January 26, 2015 1:47 PM, "Algeo, Lacie A" wrote: Hi All! Does anyone know of a standard that indicates how many cross sections must be evaluated on a temp art?? Also, any standard on running an elastic stain routinely?? Currently, we are cutting our temp arts into 8 or so pieces per cassette.? After that we do 6 slides with serial sections.? So, we're looking at approximately 240 cross sections. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law.? If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message.? If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdwyatt <@t> vet.k-state.edu Tue Jan 27 06:52:18 2015 From: kdwyatt <@t> vet.k-state.edu (Kristina Wyatt) Date: Tue Jan 27 06:52:25 2015 Subject: [Histonet] Laboratory Technician II- KSVDL job posting Message-ID: <1422363138855.72969@vet.k-state.edu> Laboratory Technician II - KSVDL The Kansas State Veterinary Diagnostic Laboratory is hiring a full-time Laboratory Technician for the Histopathology/Immunohistochemistry laboratory. This person will perform histology and general laboratory duties related to service and research. Duties will include trimming (grossing) of fixed biopsy and necropsy tissues, cutting tissues on a microtome and preparing glass slides of the tissues in accordance with those QA/QC guidelines of an AAVLD accredited laboratory. Also, routine filing of slides, tissue blocks, data entry, operating and maintaining automated histology equipment, immunohistochemical staining, and operation and organization of digital pathology projects will be required. Significant attention must be given to the KSVDL QMS (Quality Management System) and subsequent compliance. A Bachelor of Science degree in biology or related field along with a minimum of six months of laboratory experience is required. Please send your letter of interest, resume or curriculum vitae, and contact information for three professional references to: Aubrey Baldwin, aubreyb85@vet.k-state.edu Screening of applicants will begin on February 2, 2015 and will continue until the position is filled. KSU is an equal opportunity employer of individuals with disabilities and protected veterans. Background check is required. Kristina Wyatt, M.A. Supervisor: Histopathology/Immunohistochemistry L216 Mosier Hall Kansas State Veterinary Diagnostic Laboratory 785-532-4464 kdwyatt@vet.k-state.edu From Lesley.Bechtold <@t> jax.org Tue Jan 27 09:21:22 2015 From: Lesley.Bechtold <@t> jax.org (Lesley Bechtold) Date: Tue Jan 27 09:21:33 2015 Subject: [Histonet] Capacity for Histotechnologists Message-ID: <08997064E2075247A4DD5A035DB51CDF657BCA70@jaxbhexms01.jax.org> Good Morning, How many of you are experiencing Winter Storm Juno? We're currently getting hit by it here in Maine! Last year, I found paper that included the recommendation by the ASCP on the average number of slides per year that a Histotechnologist could be expected to deliver. I can't find the paper. Does anyone know where I can look it up? Or any similar papers? Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From debbie.granato <@t> urologyaustin.com Mon Jan 26 11:07:01 2015 From: debbie.granato <@t> urologyaustin.com (Debbie Granato) Date: Tue Jan 27 10:26:30 2015 Subject: [Histonet] Cassette Labeler Message-ID: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> Our lab is currently looking into purchasing a cassette and slide labeler. We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. I would appreciate any feedback or suggestions for any model that may fulfill our needs. Thank you, Debbie Granato HT(ASCP) From mpence <@t> grhs.net Tue Jan 27 10:32:06 2015 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Jan 27 10:32:41 2015 Subject: [Histonet] RE: Cassette Labeler In-Reply-To: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> References: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> Message-ID: Look at the SIideMate and PrintMate from Thermofisher. This is what we started with then intergraded into our LIS this year. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Tuesday, January 27, 2015 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler Our lab is currently looking into purchasing a cassette and slide labeler. We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. I would appreciate any feedback or suggestions for any model that may fulfill our needs. Thank you, Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Jan 27 10:33:37 2015 From: jqb7 <@t> cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Tue Jan 27 10:34:00 2015 Subject: [Histonet] RE: Cassette Labeler In-Reply-To: References: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> Message-ID: <3B2CD438E1628A41BD687E98B963B7812EBC0D22@EMBX-CLFT4.cdc.gov> Sakura also has a similar system -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, January 27, 2015 11:32 AM To: 'Debbie Granato'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cassette Labeler Look at the SIideMate and PrintMate from Thermofisher. This is what we started with then intergraded into our LIS this year. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Tuesday, January 27, 2015 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler Our lab is currently looking into purchasing a cassette and slide labeler. We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. I would appreciate any feedback or suggestions for any model that may fulfill our needs. Thank you, Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mpence <@t> grhs.net Tue Jan 27 10:35:03 2015 From: mpence <@t> grhs.net (Mike Pence) Date: Tue Jan 27 10:35:28 2015 Subject: [Histonet] RE: Cassette Labeler In-Reply-To: <3B2CD438E1628A41BD687E98B963B7812EBC0D22@EMBX-CLFT4.cdc.gov> References: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> <3B2CD438E1628A41BD687E98B963B7812EBC0D22@EMBX-CLFT4.cdc.gov> Message-ID: I would look into any of them. They work great. -----Original Message----- From: Sanders, Jeanine (CDC/OID/NCEZID) [mailto:jqb7@cdc.gov] Sent: Tuesday, January 27, 2015 10:34 AM To: Mike Pence; 'Debbie Granato'; histonet@lists.utsouthwestern.edu Subject: RE: Cassette Labeler Sakura also has a similar system -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, January 27, 2015 11:32 AM To: 'Debbie Granato'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cassette Labeler Look at the SIideMate and PrintMate from Thermofisher. This is what we started with then intergraded into our LIS this year. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Tuesday, January 27, 2015 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler Our lab is currently looking into purchasing a cassette and slide labeler. We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. I would appreciate any feedback or suggestions for any model that may fulfill our needs. Thank you, Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Herrick.James <@t> mayo.edu Tue Jan 27 10:34:56 2015 From: Herrick.James <@t> mayo.edu (Herrick, James L. (Jim)) Date: Tue Jan 27 10:36:48 2015 Subject: [Histonet] Issues with rat mandible sectioning and staining Message-ID: <59dacd$knadle@ironport9.mayo.edu> Good morning Everyone!! We have embedded several rat mandibles in MMA (we add Perkadox to the MMA to initiate) but are having issues with sectioning. We would like to keep the sections close to a 5 um thickness and need to stain with an H&E. Unfortunately, we are having issues with sectioning and staining. The sections do not look too bad coming off of the microtome, but following attachment, deplasticization and staining, the sections do not stay together very well making it very difficult for our pathologist to evaluate. Any advice on how to improve our quality on these samples would be greatly appreciated. Thank you so much!! Jim From histologygeek <@t> comcast.net Tue Jan 27 10:46:42 2015 From: histologygeek <@t> comcast.net (Histology Geek) Date: Tue Jan 27 10:47:05 2015 Subject: [Histonet] Capacity for Histotechnologists In-Reply-To: <08997064E2075247A4DD5A035DB51CDF657BCA70@jaxbhexms01.jax.org> References: <08997064E2075247A4DD5A035DB51CDF657BCA70@jaxbhexms01.jax.org> Message-ID: <9C5CEE4D-4EC0-4BE7-B180-A1B60267B521@comcast.net> Hi, Here is the article that I've used in the past. http://www.histosearch.com/ADP9ProductivityStandards.pdf > On Jan 27, 2015, at 10:21 AM, Lesley Bechtold wrote: > > Good Morning, > > How many of you are experiencing Winter Storm Juno? We're currently getting hit by it here in Maine! > > Last year, I found paper that included the recommendation by the ASCP on the average number of slides per year that a Histotechnologist could be expected to deliver. I can't find the paper. Does anyone know where I can look it up? Or any similar papers? > > Thank you. > > Lesley > > > Lesley S. Bechtold > Senior Manager, Histopathology Sciences > The Jackson Laboratory > 600 Main St. > Bar Harbor, ME 04609 > 207-288-6322 (phone) > 207-288-6325 (fax) > > > The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjones <@t> metropath.com Tue Jan 27 10:47:44 2015 From: mjones <@t> metropath.com (Michael Ann Jones) Date: Tue Jan 27 10:47:51 2015 Subject: [Histonet] Re: Electronic monitoring systems In-Reply-To: <761E2B5697F795489C8710BCC72141FF367ED862@ex07.net.ucsf.edu> References: <761E2B5697F795489C8710BCC72141FF367ED862@ex07.net.ucsf.edu> Message-ID: Thanks for everyone?s input! Michael Ann On 1/20/15, 12:21 PM, "Morken, Timothy" wrote: >We use Sensaphone for our older VIP5 tissue processors, Leica online >system for the Peloris processors and Awarepoint for >refrigerators/freezers. > >Tim Morken >Supervisor, Histology, Electron Microscopy and Neuromuscular Special >Studies >UC San Francisco Medical Center >San Francisco, CA > >CONFIDENTIALITY NOTICE: This email message, including any attachments, is >for the sole use of the intended recipient(s) and may contain >confidential, proprietary, and/or privileged information protected by >law. If you are not the intended recipient, you may not use, copy, or >distribute this email message or its attachments. If you believe you have >received this email message in error, please contact the sender by reply >email and destroy all copies of the original message. > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael >Ann Jones >Sent: Tuesday, January 20, 2015 11:13 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Electronic monitoring systems > >Hi all, >I was wondering - a little while ago there was mention on the blog of >equipment (processors) being monitored and alerting the 'manager' if they >went down. I'm sorry I cannot remember what system this was. What do you >all use? >Also, what temperature control monitor is everyone using? Ours has flaws >and we are gathering information. >Can the two systems be combined under one company? >Any help is appreciated. > >Thank you! >Michael Ann >Michael Ann Jones, HT (ASCP) >Histology Manager >Metropath >7444 W. Alaska Dr. #250 >Lakewood, CO 80226 >303.634.2511 >Mjones@metropath.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper <@t> chla.usc.edu Tue Jan 27 10:50:54 2015 From: bcooper <@t> chla.usc.edu (Cooper, Brian) Date: Tue Jan 27 10:51:17 2015 Subject: [Histonet] RE: Cassette Labeler In-Reply-To: References: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> <3B2CD438E1628A41BD687E98B963B7812EBC0D22@EMBX-CLFT4.cdc.gov> Message-ID: Leica's IPC cassette printers are workhorses. This is the only type I've ever had in the labs I've worked in, so I can't comment on any others. Brian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, January 27, 2015 8:35 AM To: 'Sanders, Jeanine (CDC/OID/NCEZID)'; 'Debbie Granato'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cassette Labeler I would look into any of them. They work great. -----Original Message----- From: Sanders, Jeanine (CDC/OID/NCEZID) [mailto:jqb7@cdc.gov] Sent: Tuesday, January 27, 2015 10:34 AM To: Mike Pence; 'Debbie Granato'; histonet@lists.utsouthwestern.edu Subject: RE: Cassette Labeler Sakura also has a similar system -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, January 27, 2015 11:32 AM To: 'Debbie Granato'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cassette Labeler Look at the SIideMate and PrintMate from Thermofisher. This is what we started with then intergraded into our LIS this year. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Tuesday, January 27, 2015 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler Our lab is currently looking into purchasing a cassette and slide labeler. We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. I would appreciate any feedback or suggestions for any model that may fulfill our needs. Thank you, Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- From relia1 <@t> earthlink.net Tue Jan 27 10:54:09 2015 From: relia1 <@t> earthlink.net (Pam Barker) Date: Tue Jan 27 10:54:18 2015 Subject: [Histonet] RELIA Histology Careers Bulletin 1-27-2015 Stay Safe and Warm During Juno! Message-ID: <000001d03a51$ded1d580$9c758080$@earthlink.net> Hi Histonetters!! I hope you are having a great day and if you are dealing with Juno I hope you are hunkered down and bundled up or having an awesome SNOWBALL FIGHT!!! In my next e-mail I will be listing information for 2015 State and Regional Histology Society Meetings. If you would like the link to your website and or meeting info to be mentioned in the 02/02/2015 RELIA Careers Bulletin Please email it to RELIA1@earthlink.net by 02/01/2015 Here is the latest update of the positions I am most excited to represent. If you are interested in any of these positions ? give me a call toll free at 866-607-3542 or shoot me an e-mail at relia1@earthlink.net and let?s discuss it. Whether you are looking for a new position today, tomorrow or 6 months from now, it is never too early to have me keep a watch out for that perfect job for you! *These Are Our Current Opportunities* Histology Management AP Manager ? Chicago, IL Histology Supervisor ? Flagstaff, AZ Lead Histotech ? Ft. Collins, CO Histology Supervisor/Research ? Boston, MA Histotechnician/Histotechnologist Histology Tech ? Norfolk, VA ? 15K SIGN ON BONUS Histology Tech ? Williamsburg, VA ? 15K SIGN ON BONUS Mohs Histotech ? Gig Harbor, WA Histotechnician ? Hammond, IN Histotechnician ? Kingsport, TN IHC Technologist ? Long Island, NY Histotech/Research ? Boston, MA Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From histologygeek <@t> comcast.net Tue Jan 27 11:02:37 2015 From: histologygeek <@t> comcast.net (Histology Geek) Date: Tue Jan 27 11:02:57 2015 Subject: [Histonet] Capacity for Histotechnologists In-Reply-To: <9C5CEE4D-4EC0-4BE7-B180-A1B60267B521@comcast.net> References: <08997064E2075247A4DD5A035DB51CDF657BCA70@jaxbhexms01.jax.org> <9C5CEE4D-4EC0-4BE7-B180-A1B60267B521@comcast.net> Message-ID: <4EDD3587-AD6E-4AAC-A8A8-CD1B48B91B22@comcast.net> And this is the one that CAP came out with based on data from NSH www.nsh.org/sites/default/files/workload.2011.pdf > On Jan 27, 2015, at 11:46 AM, Histology Geek wrote: > > Hi, > > Here is the article that I've used in the past. > http://www.histosearch.com/ADP9ProductivityStandards.pdf > > > > >> On Jan 27, 2015, at 10:21 AM, Lesley Bechtold wrote: >> >> Good Morning, >> >> How many of you are experiencing Winter Storm Juno? We're currently getting hit by it here in Maine! >> >> Last year, I found paper that included the recommendation by the ASCP on the average number of slides per year that a Histotechnologist could be expected to deliver. I can't find the paper. Does anyone know where I can look it up? Or any similar papers? >> >> Thank you. >> >> Lesley >> >> >> Lesley S. Bechtold >> Senior Manager, Histopathology Sciences >> The Jackson Laboratory >> 600 Main St. >> Bar Harbor, ME 04609 >> 207-288-6322 (phone) >> 207-288-6325 (fax) >> >> >> The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Tue Jan 27 11:05:02 2015 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Tue Jan 27 11:05:18 2015 Subject: [Histonet] RE: Cassette Labeler In-Reply-To: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> References: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> Message-ID: We use General Data for cassettes and slide labels and are very happy with it. We are adding Cytology to it this year. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Monday, January 26, 2015 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler Our lab is currently looking into purchasing a cassette and slide labeler. We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. I would appreciate any feedback or suggestions for any model that may fulfill our needs. Thank you, Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. From hfedor <@t> jhmi.edu Tue Jan 27 11:25:22 2015 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Tue Jan 27 11:25:28 2015 Subject: [Histonet] RE: Cassette Labeler In-Reply-To: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> References: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> Message-ID: Hello, we recently purchased the Primera unit from Creative waste solutions. http://www.cwsincorp.com/ we like it. I would be happy to discuss it further if you like. Helen L. Fedor Oncology Tissue Services, Manager Johns Hopkins University 411 N. Caroline St? Room 310 Basement| Bond St Annex Building Baltimore, MD?| 21231 410-614-1660 http://tmalab.jhmi.edu/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Monday, January 26, 2015 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler Our lab is currently looking into purchasing a cassette and slide labeler. We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. I would appreciate any feedback or suggestions for any model that may fulfill our needs. Thank you, Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Jan 27 12:08:24 2015 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Jan 27 12:08:47 2015 Subject: [Histonet] RE: Cassette Labeler In-Reply-To: References: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> Message-ID: <5A2BD13465E061429D6455C8D6B40E39173192DD96@IBMB7Exchange.digestivespecialists.com> I second that! I love mine. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Tuesday, January 27, 2015 12:25 PM To: Debbie Granato; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cassette Labeler Hello, we recently purchased the Primera unit from Creative waste solutions. http://www.cwsincorp.com/ we like it. I would be happy to discuss it further if you like. Helen L. Fedor Oncology Tissue Services, Manager Johns Hopkins University 411 N. Caroline St Room 310 Basement| Bond St Annex Building Baltimore, MD?| 21231 410-614-1660 http://tmalab.jhmi.edu/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Monday, January 26, 2015 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler Our lab is currently looking into purchasing a cassette and slide labeler. We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. I would appreciate any feedback or suggestions for any model that may fulfill our needs. Thank you, Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.auge <@t> gmail.com Tue Jan 27 12:10:58 2015 From: tony.auge <@t> gmail.com (Tony Auge) Date: Tue Jan 27 12:11:06 2015 Subject: [Histonet] RE: Cassette Labeler In-Reply-To: References: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> Message-ID: I also have a Primera slide printer. It worked well at first but now it is 2 years old and prints very slowly. I can hand write up slides faster than they print. I will be in the market for a new slide printer soon and will not be going back to Primera. -- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.auge@gmail.com From bcdukes <@t> lexhealth.org Tue Jan 27 12:14:44 2015 From: bcdukes <@t> lexhealth.org (Blake Taylor) Date: Tue Jan 27 12:15:02 2015 Subject: [Histonet] Cassette Labeler In-Reply-To: <201501271801.t0RI1V9p031485@rly39g.srv.mailcontrol.com> References: <201501271801.t0RI1V9p031485@rly39g.srv.mailcontrol.com> Message-ID: We have the Thermo Printmate and is has been a good solution for us. We used it before we integrated to bar coding and during LIS down times it is easy to switch back to a more manual method. I would recommend it. Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes@lexhealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, January 27, 2015 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 134, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. temporal arteries (Algeo, Lacie A) 2. Re: temporal arteries (Rene J Buesa) 3. Laboratory Technician II- KSVDL job posting (Kristina Wyatt) 4. Capacity for Histotechnologists (Lesley Bechtold) 5. Cassette Labeler (Debbie Granato) 6. RE: Cassette Labeler (Mike Pence) 7. RE: Cassette Labeler (Sanders, Jeanine (CDC/OID/NCEZID)) 8. RE: Cassette Labeler (Mike Pence) 9. Issues with rat mandible sectioning and staining (Herrick, James L. (Jim)) 10. Re: Capacity for Histotechnologists (Histology Geek) 11. Re: Electronic monitoring systems (Michael Ann Jones) 12. RE: Cassette Labeler (Cooper, Brian) 13. RELIA Histology Careers Bulletin 1-27-2015 Stay Safe and Warm During Juno! (Pam Barker) 14. Re: Capacity for Histotechnologists (Histology Geek) 15. RE: Cassette Labeler (Tom McNemar) 16. RE: Cassette Labeler (Helen Fedor) ---------------------------------------------------------------------- Message: 1 Date: Mon, 26 Jan 2015 18:46:43 +0000 From: "Algeo, Lacie A" Subject: [Histonet] temporal arteries To: "histonet@lists.utsouthwestern.edu" Message-ID: <24C4B3C167E5694887AB594C7602CE3A03B8406B@WN35104.or.providence.org> Content-Type: text/plain; charset="us-ascii" Hi All! Does anyone know of a standard that indicates how many cross sections must be evaluated on a temp art? Also, any standard on running an elastic stain routinely? Currently, we are cutting our temp arts into 8 or so pieces per cassette. After that we do 6 slides with serial sections. So, we're looking at approximately 240 cross sections. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law. If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. ------------------------------ Message: 2 Date: Mon, 26 Jan 2015 21:23:51 +0000 (UTC) From: Rene J Buesa Subject: Re: [Histonet] temporal arteries To: "Algeo, Lacie A" , "histonet@lists.utsouthwestern.edu" Message-ID: <1450715820.490812.1422307431439.JavaMail.yahoo@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 There are no standards about this issue and all will depend on what your pathologist wants to screen.Remember that SUPPOSEDLY your PT will have to screen avery thing you??do.Ask your PT in what is s/he wants to screen to feel "comfortable" with the diagnosis.Ren?? J.?? On Monday, January 26, 2015 1:47 PM, "Algeo, Lacie A" wrote: Hi All! Does anyone know of a standard that indicates how many cross sections must be evaluated on a temp art??? Also, any standard on running an elastic stain routinely??? Currently, we are cutting our temp arts into 8 or so pieces per cassette.?? After that we do 6 slides with serial sections.?? So, we're looking at approximately 240 cross sections. Thanks, Lacie Lacie Algeo, HTL (ASCP) MBCM Histology Supervisor Providence Sacred Heart Medical Center Laboratory 101 W 8th Avenue L-2 Spokane, WA 99204 509-474-4418 FAX 509-474-2052 lacie.algeo@providence.org This message is intended for the sole use of the addressee, and may contain information that is priviledged, confidential and exempt from disclosure under applicable law.?? If you are not the addressee, you are hereby notified that you may not use, copy, disclose or distribute to anyone the message or any information contained in the message.?? If you have received this message in error, please immediately advise the sender by reply e-mail and delete this message. ________________________________ This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 27 Jan 2015 12:52:18 +0000 From: Kristina Wyatt Subject: [Histonet] Laboratory Technician II- KSVDL job posting To: "histonet@lists.utsouthwestern.edu" Message-ID: <1422363138855.72969@vet.k-state.edu> Content-Type: text/plain; charset="iso-8859-1" Laboratory Technician II - KSVDL The Kansas State Veterinary Diagnostic Laboratory is hiring a full-time Laboratory Technician for the Histopathology/Immunohistochemistry laboratory. This person will perform histology and general laboratory duties related to service and research. Duties will include trimming (grossing) of fixed biopsy and necropsy tissues, cutting tissues on a microtome and preparing glass slides of the tissues in accordance with those QA/QC guidelines of an AAVLD accredited laboratory. Also, routine filing of slides, tissue blocks, data entry, operating and maintaining automated histology equipment, immunohistochemical staining, and operation and organization of digital pathology projects will be required. Significant attention must be given to the KSVDL QMS (Quality Management System) and subsequent compliance. A Bachelor of Science degree in biology or related field along with a minimum of six months of laboratory experience is required. Please send your letter of interest, resume or curriculum vitae, and contact information for three professional references to: Aubrey Baldwin, aubreyb85@vet.k-state.edu Screening of applicants will begin on February 2, 2015 and will continue until the position is filled. KSU is an equal opportunity employer of individuals with disabilities and protected veterans. Background check is required. Kristina Wyatt, M.A. Supervisor: Histopathology/Immunohistochemistry L216 Mosier Hall Kansas State Veterinary Diagnostic Laboratory 785-532-4464 kdwyatt@vet.k-state.edu ------------------------------ Message: 4 Date: Tue, 27 Jan 2015 15:21:22 +0000 From: Lesley Bechtold Subject: [Histonet] Capacity for Histotechnologists To: "histonet@lists.utsouthwestern.edu" Message-ID: <08997064E2075247A4DD5A035DB51CDF657BCA70@jaxbhexms01.jax.org> Content-Type: text/plain; charset="us-ascii" Good Morning, How many of you are experiencing Winter Storm Juno? We're currently getting hit by it here in Maine! Last year, I found paper that included the recommendation by the ASCP on the average number of slides per year that a Histotechnologist could be expected to deliver. I can't find the paper. Does anyone know where I can look it up? Or any similar papers? Thank you. Lesley Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ------------------------------ Message: 5 Date: Mon, 26 Jan 2015 17:07:01 +0000 From: Debbie Granato Subject: [Histonet] Cassette Labeler To: "histonet@lists.utsouthwestern.edu" Message-ID: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> Content-Type: text/plain; charset="us-ascii" Our lab is currently looking into purchasing a cassette and slide labeler. We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. I would appreciate any feedback or suggestions for any model that may fulfill our needs. Thank you, Debbie Granato HT(ASCP) ------------------------------ Message: 6 Date: Tue, 27 Jan 2015 16:32:06 +0000 From: Mike Pence Subject: [Histonet] RE: Cassette Labeler To: 'Debbie Granato' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" Look at the SIideMate and PrintMate from Thermofisher. This is what we started with then intergraded into our LIS this year. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Tuesday, January 27, 2015 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler Our lab is currently looking into purchasing a cassette and slide labeler. We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. I would appreciate any feedback or suggestions for any model that may fulfill our needs. Thank you, Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 27 Jan 2015 16:33:37 +0000 From: "Sanders, Jeanine (CDC/OID/NCEZID)" Subject: [Histonet] RE: Cassette Labeler To: Mike Pence , 'Debbie Granato' , "histonet@lists.utsouthwestern.edu" Message-ID: <3B2CD438E1628A41BD687E98B963B7812EBC0D22@EMBX-CLFT4.cdc.gov> Content-Type: text/plain; charset="us-ascii" Sakura also has a similar system -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, January 27, 2015 11:32 AM To: 'Debbie Granato'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cassette Labeler Look at the SIideMate and PrintMate from Thermofisher. This is what we started with then intergraded into our LIS this year. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Tuesday, January 27, 2015 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler Our lab is currently looking into purchasing a cassette and slide labeler. We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. I would appreciate any feedback or suggestions for any model that may fulfill our needs. Thank you, Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 27 Jan 2015 16:35:03 +0000 From: Mike Pence Subject: [Histonet] RE: Cassette Labeler To: "'Sanders, Jeanine (CDC/OID/NCEZID)'" , 'Debbie Granato' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" I would look into any of them. They work great. -----Original Message----- From: Sanders, Jeanine (CDC/OID/NCEZID) [mailto:jqb7@cdc.gov] Sent: Tuesday, January 27, 2015 10:34 AM To: Mike Pence; 'Debbie Granato'; histonet@lists.utsouthwestern.edu Subject: RE: Cassette Labeler Sakura also has a similar system -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, January 27, 2015 11:32 AM To: 'Debbie Granato'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cassette Labeler Look at the SIideMate and PrintMate from Thermofisher. This is what we started with then intergraded into our LIS this year. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Tuesday, January 27, 2015 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler Our lab is currently looking into purchasing a cassette and slide labeler. We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. I would appreciate any feedback or suggestions for any model that may fulfill our needs. Thank you, Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 27 Jan 2015 16:34:56 +0000 From: "Herrick, James L. (Jim)" Subject: [Histonet] Issues with rat mandible sectioning and staining To: "histonet@lists.utsouthwestern.edu" Message-ID: <59dacd$knadle@ironport9.mayo.edu> Content-Type: text/plain; charset="us-ascii" Good morning Everyone!! We have embedded several rat mandibles in MMA (we add Perkadox to the MMA to initiate) but are having issues with sectioning. We would like to keep the sections close to a 5 um thickness and need to stain with an H&E. Unfortunately, we are having issues with sectioning and staining. The sections do not look too bad coming off of the microtome, but following attachment, deplasticization and staining, the sections do not stay together very well making it very difficult for our pathologist to evaluate. Any advice on how to improve our quality on these samples would be greatly appreciated. Thank you so much!! Jim ------------------------------ Message: 10 Date: Tue, 27 Jan 2015 11:46:42 -0500 From: Histology Geek Subject: Re: [Histonet] Capacity for Histotechnologists To: Lesley Bechtold Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <9C5CEE4D-4EC0-4BE7-B180-A1B60267B521@comcast.net> Content-Type: text/plain; charset=us-ascii Hi, Here is the article that I've used in the past. http://www.histosearch.com/ADP9ProductivityStandards.pdf > On Jan 27, 2015, at 10:21 AM, Lesley Bechtold wrote: > > Good Morning, > > How many of you are experiencing Winter Storm Juno? We're currently getting hit by it here in Maine! > > Last year, I found paper that included the recommendation by the ASCP on the average number of slides per year that a Histotechnologist could be expected to deliver. I can't find the paper. Does anyone know where I can look it up? Or any similar papers? > > Thank you. > > Lesley > > > Lesley S. Bechtold > Senior Manager, Histopathology Sciences > The Jackson Laboratory > 600 Main St. > Bar Harbor, ME 04609 > 207-288-6322 (phone) > 207-288-6325 (fax) > > > The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Tue, 27 Jan 2015 16:47:44 +0000 From: Michael Ann Jones Subject: [Histonet] Re: Electronic monitoring systems To: "Morken, Timothy" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Thanks for everyone?s input! Michael Ann On 1/20/15, 12:21 PM, "Morken, Timothy" wrote: >We use Sensaphone for our older VIP5 tissue processors, Leica online >system for the Peloris processors and Awarepoint for >refrigerators/freezers. > >Tim Morken >Supervisor, Histology, Electron Microscopy and Neuromuscular Special >Studies >UC San Francisco Medical Center >San Francisco, CA > >CONFIDENTIALITY NOTICE: This email message, including any attachments, is >for the sole use of the intended recipient(s) and may contain >confidential, proprietary, and/or privileged information protected by >law. If you are not the intended recipient, you may not use, copy, or >distribute this email message or its attachments. If you believe you have >received this email message in error, please contact the sender by reply >email and destroy all copies of the original message. > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michael >Ann Jones >Sent: Tuesday, January 20, 2015 11:13 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Electronic monitoring systems > >Hi all, >I was wondering - a little while ago there was mention on the blog of >equipment (processors) being monitored and alerting the 'manager' if they >went down. I'm sorry I cannot remember what system this was. What do you >all use? >Also, what temperature control monitor is everyone using? Ours has flaws >and we are gathering information. >Can the two systems be combined under one company? >Any help is appreciated. > >Thank you! >Michael Ann >Michael Ann Jones, HT (ASCP) >Histology Manager >Metropath >7444 W. Alaska Dr. #250 >Lakewood, CO 80226 >303.634.2511 >Mjones@metropath.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 27 Jan 2015 16:50:54 +0000 From: "Cooper, Brian" Subject: [Histonet] RE: Cassette Labeler To: "'Debbie Granato'" , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset=us-ascii Leica's IPC cassette printers are workhorses. This is the only type I've ever had in the labs I've worked in, so I can't comment on any others. Brian -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, January 27, 2015 8:35 AM To: 'Sanders, Jeanine (CDC/OID/NCEZID)'; 'Debbie Granato'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cassette Labeler I would look into any of them. They work great. -----Original Message----- From: Sanders, Jeanine (CDC/OID/NCEZID) [mailto:jqb7@cdc.gov] Sent: Tuesday, January 27, 2015 10:34 AM To: Mike Pence; 'Debbie Granato'; histonet@lists.utsouthwestern.edu Subject: RE: Cassette Labeler Sakura also has a similar system -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Pence Sent: Tuesday, January 27, 2015 11:32 AM To: 'Debbie Granato'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cassette Labeler Look at the SIideMate and PrintMate from Thermofisher. This is what we started with then intergraded into our LIS this year. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Tuesday, January 27, 2015 10:28 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler Our lab is currently looking into purchasing a cassette and slide labeler. We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. I would appreciate any feedback or suggestions for any model that may fulfill our needs. Thank you, Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------------- ------------------------------ Message: 13 Date: Tue, 27 Jan 2015 11:54:09 -0500 From: "Pam Barker" Subject: [Histonet] RELIA Histology Careers Bulletin 1-27-2015 Stay Safe and Warm During Juno! To: "Histonet" Message-ID: <000001d03a51$ded1d580$9c758080$@earthlink.net> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters!! I hope you are having a great day and if you are dealing with Juno I hope you are hunkered down and bundled up or having an awesome SNOWBALL FIGHT!!! In my next e-mail I will be listing information for 2015 State and Regional Histology Society Meetings. If you would like the link to your website and or meeting info to be mentioned in the 02/02/2015 RELIA Careers Bulletin Please email it to RELIA1@earthlink.net by 02/01/2015 Here is the latest update of the positions I am most excited to represent. If you are interested in any of these positions ? give me a call toll free at 866-607-3542 or shoot me an e-mail at relia1@earthlink.net and let?s discuss it. Whether you are looking for a new position today, tomorrow or 6 months from now, it is never too early to have me keep a watch out for that perfect job for you! *These Are Our Current Opportunities* Histology Management AP Manager ? Chicago, IL Histology Supervisor ? Flagstaff, AZ Lead Histotech ? Ft. Collins, CO Histology Supervisor/Research ? Boston, MA Histotechnician/Histotechnologist Histology Tech ? Norfolk, VA ? 15K SIGN ON BONUS Histology Tech ? Williamsburg, VA ? 15K SIGN ON BONUS Mohs Histotech ? Gig Harbor, WA Histotechnician ? Hammond, IN Histotechnician ? Kingsport, TN IHC Technologist ? Long Island, NY Histotech/Research ? Boston, MA Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 14 Date: Tue, 27 Jan 2015 12:02:37 -0500 From: Histology Geek Subject: Re: [Histonet] Capacity for Histotechnologists To: Lesley Bechtold Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <4EDD3587-AD6E-4AAC-A8A8-CD1B48B91B22@comcast.net> Content-Type: text/plain; charset=us-ascii And this is the one that CAP came out with based on data from NSH www.nsh.org/sites/default/files/workload.2011.pdf > On Jan 27, 2015, at 11:46 AM, Histology Geek wrote: > > Hi, > > Here is the article that I've used in the past. > http://www.histosearch.com/ADP9ProductivityStandards.pdf > > > > >> On Jan 27, 2015, at 10:21 AM, Lesley Bechtold wrote: >> >> Good Morning, >> >> How many of you are experiencing Winter Storm Juno? We're currently getting hit by it here in Maine! >> >> Last year, I found paper that included the recommendation by the ASCP on the average number of slides per year that a Histotechnologist could be expected to deliver. I can't find the paper. Does anyone know where I can look it up? Or any similar papers? >> >> Thank you. >> >> Lesley >> >> >> Lesley S. Bechtold >> Senior Manager, Histopathology Sciences >> The Jackson Laboratory >> 600 Main St. >> Bar Harbor, ME 04609 >> 207-288-6322 (phone) >> 207-288-6325 (fax) >> >> >> The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Tue, 27 Jan 2015 12:05:02 -0500 From: Tom McNemar Subject: [Histonet] RE: Cassette Labeler To: 'Debbie Granato' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="us-ascii" We use General Data for cassettes and slide labels and are very happy with it. We are adding Cytology to it this year. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Monday, January 26, 2015 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler Our lab is currently looking into purchasing a cassette and slide labeler. We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. I would appreciate any feedback or suggestions for any model that may fulfill our needs. Thank you, Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 16 Date: Tue, 27 Jan 2015 17:25:22 +0000 From: Helen Fedor Subject: [Histonet] RE: Cassette Labeler To: Debbie Granato , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello, we recently purchased the Primera unit from Creative waste solutions. http://www.cwsincorp.com/ we like it. I would be happy to discuss it further if you like. Helen L. Fedor Oncology Tissue Services, Manager Johns Hopkins University 411 N. Caroline St? Room 310 Basement| Bond St Annex Building Baltimore, MD?| 21231 410-614-1660 http://tmalab.jhmi.edu/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debbie Granato Sent: Monday, January 26, 2015 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette Labeler Our lab is currently looking into purchasing a cassette and slide labeler. We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. I would appreciate any feedback or suggestions for any model that may fulfill our needs. Thank you, Debbie Granato HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 134, Issue 33 ***************************************** PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use of the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. From lblazek <@t> digestivespecialists.com Tue Jan 27 12:23:13 2015 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Jan 27 12:23:22 2015 Subject: [Histonet] RE: Cassette Labeler In-Reply-To: References: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> Message-ID: <5A2BD13465E061429D6455C8D6B40E39173192DDAB@IBMB7Exchange.digestivespecialists.com> I've had mine longer than that and don't have a problem. I have a feeling that you may need a bit of service on it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Auge Sent: Tuesday, January 27, 2015 1:11 PM To: Helen Fedor Cc: histonet@lists.utsouthwestern.edu; Debbie Granato Subject: Re: [Histonet] RE: Cassette Labeler I also have a Primera slide printer. It worked well at first but now it is 2 years old and prints very slowly. I can hand write up slides faster than they print. I will be in the market for a new slide printer soon and will not be going back to Primera. -- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.auge@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From craigak12 <@t> gmail.com Tue Jan 27 12:24:21 2015 From: craigak12 <@t> gmail.com (Jb) Date: Tue Jan 27 12:24:33 2015 Subject: [Histonet] Bloodborne pathogens/Safety Training: Message-ID: <601A0F5D-7965-4C8D-A00C-550DAF93577E@gmail.com> I need to enroll a small laboratory in BBP/safety training for CAP/CLIA standards. Can anyone advise a good company for online training? Thank you, Craig Sent from my iPhone From amanda.krempley <@t> abbvie.com Tue Jan 27 12:40:00 2015 From: amanda.krempley <@t> abbvie.com (Krempley, Amanda L) Date: Tue Jan 27 12:40:14 2015 Subject: [Histonet] RE: Cassette Labeler In-Reply-To: <5A2BD13465E061429D6455C8D6B40E39173192DDAB@IBMB7Exchange.digestivespecialists.com> References: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> <5A2BD13465E061429D6455C8D6B40E39173192DDAB@IBMB7Exchange.digestivespecialists.com> Message-ID: <37b93a66a5a2432b8ef4139e224d25da@USAASECSM048.R0018.COLLABORATION.ECS.HP.COM> We have 2 Primera slide printers and have had nothing but problems since the day we started using them. We usually need to print 20-40 slides at a time and almost always have a jam up while printing. We have also had a problem with the ribbon breaking. We had so many problems Primera replaced our printers with new ones and we still had the same problems. Another note you can't use any slide you want with Primera, the printing surface needs to have a certain texture for the ink to stick. For example it does not print on Leica slides but will print on IMEB slides. We demonstrated the Thermo printers and really liked them but the cassette printer didn't have a large enough output for us. We can print anywhere from 200-1000 cassettes at a time. Currently we use Leica's printers and love them. They are great work horses, we have very few problems with them and when we do have problems they are very easy to troubleshoot. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, January 27, 2015 12:23 PM To: Tony Auge; Helen Fedor Cc: histonet@lists.utsouthwestern.edu; Debbie Granato Subject: RE: [Histonet] RE: Cassette Labeler I've had mine longer than that and don't have a problem. I have a feeling that you may need a bit of service on it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Auge Sent: Tuesday, January 27, 2015 1:11 PM To: Helen Fedor Cc: histonet@lists.utsouthwestern.edu; Debbie Granato Subject: Re: [Histonet] RE: Cassette Labeler I also have a Primera slide printer. It worked well at first but now it is 2 years old and prints very slowly. I can hand write up slides faster than they print. I will be in the market for a new slide printer soon and will not be going back to Primera. -- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.auge@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From craigak12 <@t> gmail.com Tue Jan 27 12:49:57 2015 From: craigak12 <@t> gmail.com (Jb) Date: Tue Jan 27 12:50:11 2015 Subject: [Histonet] Her2: Message-ID: <68ABDFC8-A3F5-4576-A6B6-E6C31A523381@gmail.com> Can anyone give me suggestions of what they are using for Her2? What are your thoughts on FDA cleared kits vs ASR? Good products/cost efficient....general thoughts. Also, does anyone know or have any literature stating that an FDA cleared kit has to be used on a specific platform that it is specified for? I have heard that an FDA cleared test has to be used on the company's specific platform to maintain the FDA status. Does anyone have info/literature supporting this? Thank you, Craig Sent from my iPhone From mike <@t> pathview.com Tue Jan 27 12:54:13 2015 From: mike <@t> pathview.com (Michael Mihalik) Date: Tue Jan 27 12:54:29 2015 Subject: [Histonet] RE: Cassette Labeler In-Reply-To: <37b93a66a5a2432b8ef4139e224d25da@USAASECSM048.R0018.COLLABORATION.ECS.HP.COM> References: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> <5A2BD13465E061429D6455C8D6B40E39173192DDAB@IBMB7Exchange.digestivespecialists.com> <37b93a66a5a2432b8ef4139e224d25da@USAASECSM048.R0018.COLLABORATION.ECS.HP.COM> Message-ID: <00e901d03a62$a5ce4870$f16ad950$@pathview.com> I think this post along with all the others illustrate a few points... 1. There are many more alternatives available for slide and cassette printers than there were even just a few years ago. I'd be curious if costs have come down. Cassette labelers used to be 25,000 and slide printers (not labelers) were 5,000. 2. Almost everyone likes what they currently have. It's difficult to have real world experience with more than 1 version of these products. 3. Just because one vendor's products work well in one environment, does not mean the product will work well in yours. Look at not only laboratory volumes, but timeframes in which those volumes were produced. 4. As in the clinical laboratory, 'reagent cost' can be a factor. Do you have to use certain slides or cassettes? What are the costs? 5. Also, 'as usual', every vendor can produce the isolated lemon. How well does that company handle the issue? What's their general level of customer support to begin with? It's just interesting to note how far these items have come in the last few years. I still wish they were cheaper though. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Krempley, Amanda L Sent: Tuesday, January 27, 2015 10:40 AM To: Blazek, Linda; Tony Auge; Helen Fedor Cc: histonet@lists.utsouthwestern.edu; Debbie Granato Subject: RE: [Histonet] RE: Cassette Labeler We have 2 Primera slide printers and have had nothing but problems since the day we started using them. We usually need to print 20-40 slides at a time and almost always have a jam up while printing. We have also had a problem with the ribbon breaking. We had so many problems Primera replaced our printers with new ones and we still had the same problems. Another note you can't use any slide you want with Primera, the printing surface needs to have a certain texture for the ink to stick. For example it does not print on Leica slides but will print on IMEB slides. We demonstrated the Thermo printers and really liked them but the cassette printer didn't have a large enough output for us. We can print anywhere from 200-1000 cassettes at a time. Currently we use Leica's printers and love them. They are great work horses, we have very few problems with them and when we do have problems they are very easy to troubleshoot. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, January 27, 2015 12:23 PM To: Tony Auge; Helen Fedor Cc: histonet@lists.utsouthwestern.edu; Debbie Granato Subject: RE: [Histonet] RE: Cassette Labeler I've had mine longer than that and don't have a problem. I have a feeling that you may need a bit of service on it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tony Auge Sent: Tuesday, January 27, 2015 1:11 PM To: Helen Fedor Cc: histonet@lists.utsouthwestern.edu; Debbie Granato Subject: Re: [Histonet] RE: Cassette Labeler I also have a Primera slide printer. It worked well at first but now it is 2 years old and prints very slowly. I can hand write up slides faster than they print. I will be in the market for a new slide printer soon and will not be going back to Primera. -- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.auge@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac <@t> outlook.com Tue Jan 27 13:04:11 2015 From: wdesalvo.cac <@t> outlook.com (WILLIAM DESALVO) Date: Tue Jan 27 13:04:27 2015 Subject: [Histonet] Cassette Labeler In-Reply-To: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> References: <6584C3F1A70B5E44B76902B2332BF442700208@UA-V-EXCH2.urologyaustin.lan> Message-ID: Go one step further before you move to printer selection. Check with the vendors of tracking software and see what they have validated or recommend. Purchase a cassette printer that will work for you as you move forward in tracking the cassettes. William DeSalvo william.desalvo@sonoraquest.com 602-768-3692 Sent from my iPhone > On Jan 27, 2015, at 9:27 AM, Debbie Granato wrote: > > Our lab is currently looking into purchasing a cassette and slide labeler. > > We are a small lab and are looking for a stand- alone unit with the flexibility to be used with the computer or bar code scanner at a later date. > > I would appreciate any feedback or suggestions for any model that may fulfill our needs. > > Thank you, > > Debbie Granato HT(ASCP) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BZIMMERM <@t> gru.edu Tue Jan 27 13:13:03 2015 From: BZIMMERM <@t> gru.edu (Zimmerman, Billie) Date: Tue Jan 27 13:17:15 2015 Subject: [Histonet] RENEW YOUR FREE GEORGIA MEMBERSHIP ! Message-ID: <7B3DEB32E69C034EACB479059C5DE3FF8DEE9A@EX-MLB-03.ad.georgiahealth.edu> http://www.histosearch.com/gsh/ Renew your free Georgia membership and all your histological dreams will come true... Yours truly, Glenda the good witch Secretary GSH From Timothy.Morken <@t> ucsf.edu Tue Jan 27 13:17:02 2015 From: Timothy.Morken <@t> ucsf.edu (Morken, Timothy) Date: Tue Jan 27 13:17:18 2015 Subject: [Histonet] Her2: In-Reply-To: <68ABDFC8-A3F5-4576-A6B6-E6C31A523381@gmail.com> References: <68ABDFC8-A3F5-4576-A6B6-E6C31A523381@gmail.com> Message-ID: <761E2B5697F795489C8710BCC72141FF367EF617@ex07.net.ucsf.edu> Craig, CLIA licensure gives your lab the authority to validate and use ASR's for diagnostics. For something like her2 you will need to compare it to an existing FDA approved antibody, run the statistically significant number of cases (range of positive, and negatives) and show high correlation between them. If you want to use an FDA approved antibody on a different platform you still need to do the validation and compare the results between the platforms. Vendors typically run many hundreds of cases for their FDA approval but most labs run 50 to 100. But with smaller numbers of cases it can be hard to get the high correlation you need (95%+). Your pathologists need to develop a validation plan and decide if it is suitable. Look at this site and go the link at the bottom for Subpart K, Part 1: http://www.cms.gov/Regulations-and-Guidance/Legislation/CLIA/Interpretive_Guidelines_for_Laboratories.html Look for section 493.1253(B)(2). Below is the intro. The standard is many pages long and tells you what you need to do to use FDA kit on a different platform than the manufacturer used for FDA certification. It is a full validation. This same standard applies to ASR's ?493.1253 Standard: Establishment and verification of performance specifications. (b)(2) Establishment of performance specifications. Each laboratory that modifies an FDA-cleared or approved test system, or introduces a test system not subject to FDA clearance or approval (including methods developed in-house and standardized methods such as text book procedures, or uses a test system in which performance specifications are not provided by the manufacturer must, before reporting patient test results, establish for each test system the performance specifications for the following performance characteristics, as applicable: Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Tuesday, January 27, 2015 10:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Her2: Can anyone give me suggestions of what they are using for Her2? What are your thoughts on FDA cleared kits vs ASR? Good products/cost efficient....general thoughts. Also, does anyone know or have any literature stating that an FDA cleared kit has to be used on a specific platform that it is specified for? I have heard that an FDA cleared test has to be used on the company's specific platform to maintain the FDA status. Does anyone have info/literature supporting this? Thank you, Craig Sent from my iPhone _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gloria.cole <@t> usa.net Tue Jan 27 14:00:17 2015 From: gloria.cole <@t> usa.net (Gloria Cole) Date: Tue Jan 27 14:00:24 2015 Subject: [Histonet] Average time for a CLIa certificate Message-ID: <314TaAT8R4288S05.1422388817@web05.cms.usa.net> Hello All, I have some folks who are interested in opening a histology laborato ry in the West Palm Beach Florida location. Can anyone in Florida give me application Thank you, Gloria From jburch01 <@t> gmail.com Tue Jan 27 14:15:53 2015 From: jburch01 <@t> gmail.com (Jim Burchette) Date: Tue Jan 27 14:16:01 2015 Subject: [Histonet] RENEW YOUR FREE GEORGIA MEMBERSHIP ! In-Reply-To: <7B3DEB32E69C034EACB479059C5DE3FF8DEE9A@EX-MLB-03.ad.georgiahealth.edu> References: <7B3DEB32E69C034EACB479059C5DE3FF8DEE9A@EX-MLB-03.ad.georgiahealth.edu> Message-ID: The limit for online responses has been met by your service. Jim On Tuesday, January 27, 2015, Zimmerman, Billie wrote: > http://www.histosearch.com/gsh/ > > Renew your free Georgia membership and all your histological dreams will > come true... > > Yours truly, > > Glenda the good witch > Secretary GSH > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jim Burchette "Fly Fishing Bum" *<'(((><* From duhrich <@t> hotmail.com Tue Jan 27 17:10:28 2015 From: duhrich <@t> hotmail.com (dianna uhrich) Date: Tue Jan 27 17:10:33 2015 Subject: [Histonet] (no subject) Message-ID: I work in a small hospital and we our having a hard time justifing the amount of people that work in our area. How do people figure this out, what do people do for FTE's Thanks for your help Dianna From duhrich <@t> hotmail.com Tue Jan 27 17:17:36 2015 From: duhrich <@t> hotmail.com (dianna uhrich) Date: Tue Jan 27 17:17:39 2015 Subject: [Histonet] FW: In-Reply-To: References: Message-ID: From: duhrich@hotmail.com To: histonet@lists.utsouthwestern.edu Subject: Date: Tue, 27 Jan 2015 23:10:28 +0000 I work in a small hospital and we our having a hard time justifing the amount of people that work in our area. How do people figure this out, what do people do for FTE's Thanks for your help Dianna From Ronald.Houston <@t> nationwidechildrens.org Wed Jan 28 11:21:51 2015 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Wed Jan 28 11:22:21 2015 Subject: [Histonet] old journal article Message-ID: Does anyone have access to old archives of Am J Clin Pathol from 1985? Looking for Sarnat, H.B., et al.: A fluorochromic stain for nuclei acids to demonstrate submucosal and myenteric neurons in Hirschsprungs disease. Amer. J. Clin. Path. 83:722-725, 1985 On-line access only goes back to 2000 Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From CDavis <@t> che-east.org Wed Jan 28 12:42:53 2015 From: CDavis <@t> che-east.org (Davis, Cassie) Date: Wed Jan 28 12:42:34 2015 Subject: [Histonet] Decalcified tissue disclaimer for IHC Message-ID: Good afternoon Histonet Folks, We now need to include a disclaimer for IHC done on decalcified tissue. (It's probably been covered here and I just woke up to it) I am not the best with words so I am asking if anybody would be kind enough to share their disclaimer. I hate to reinvent the wheel. There is always something to do and something new in Histology... Cassandra Davis CDavis@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From tawnia <@t> ampianstaffing.com Wed Jan 28 12:54:04 2015 From: tawnia <@t> ampianstaffing.com (Tawnia Lindsay) Date: Wed Jan 28 12:54:18 2015 Subject: [Histonet] Amazing Job Opportunities Message-ID: I am currently looking for direct hire and temp or temp to permanent histologists and histologist with grossing backgrounds throughout the country. I have jobs in New Mexico, Arizona, Nevada, Missouri, and Kentucky. Travel jobs all have housing, transportation and per Diems, while permanent jobs may include relocation and a sign on bonus! Please call me at 877-229-6996 ext 2009 if you are in the market for a great new opportunity. Tawnia Lindsay Ampian Staffing, Inc. 126 W Sego Lily Suite 110 Sandy UT 84070 O:877-229-6996 ext 2009 F:801-253-6127 email: tawnia@ampianstaffing.com Website: ampainstaffing.com From sforeman <@t> labpath.com Wed Jan 28 12:50:30 2015 From: sforeman <@t> labpath.com (Susan Foreman) Date: Wed Jan 28 13:00:24 2015 Subject: [Histonet] hpv Message-ID: <01ed01d03b2b$49d39560$dd7ac020$@labpath.com> Our laboratory offers HPV testing by IHC on ffpe tissues. For info please contact Joe Justice jjustice@kdlsrv6.labpath.com KDL Pathology Knoxville, TN 37919 or (865)584-1933 From wynder <@t> wisc.edu Wed Jan 28 14:55:54 2015 From: wynder <@t> wisc.edu (Jalissa Wynder) Date: Wed Jan 28 14:56:03 2015 Subject: [Histonet] GPER antibody for IHC??? In-Reply-To: <7760ef40405c4.54c94cbb@wiscmail.wisc.edu> References: <74c0dcae440cf.54c94b8d@wiscmail.wisc.edu> <74c0cf4945d21.54c94bca@wiscmail.wisc.edu> <7760eeda42d7a.54c94c06@wiscmail.wisc.edu> <7560c2424782b.54c94c43@wiscmail.wisc.edu> <7760ef40405c4.54c94cbb@wiscmail.wisc.edu> Message-ID: <73b0818947719.54c8f87a@wiscmail.wisc.edu> Hi all, Can someone recommend a company they have had experimental success using an antibody for GPER(G protein-coupled estrogen receptor 1) ? Best, Jalissa From mhale <@t> MiracaLS.com Wed Jan 28 15:41:10 2015 From: mhale <@t> MiracaLS.com (Hale, Meredith) Date: Wed Jan 28 15:41:32 2015 Subject: [Histonet] HT Position in Scottsdale , AZ Message-ID: <0E828EC51C7CC445A51E53F81B64E8C7443139A6@DFW-EXMBN2.pathologypartners.intranet> Great full time opportunity for a Histotechnician in Scottsdale, Arizona! Arizona Gastrointestinal Associates is looking for certified HT or HTL to join their laboratory. HT candidate must meet the following criteria: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens a plus * HT ASCP Certified Duties include: * Grossing * Embedding * Microtomy * Staining * Ability to be flexible and take on additional duties' as needed These positions offer a competitive rate and benefits, and schedule flexibility. Interested applicants can send resume with salary requirements to: Lisa Steirer, Administrator Arizona Gastrointestinal Associates, PLC lsteirer@azgastrohealth.com From julien.c.prevost <@t> gmail.com Wed Jan 28 20:58:29 2015 From: julien.c.prevost <@t> gmail.com (=?UTF-8?Q?Julien_Pr=C3=A9vost?=) Date: Wed Jan 28 20:58:35 2015 Subject: [Histonet] mix of questions Message-ID: Hello histonet, I am new to this list and new to the world of histology and I am seeking advice for a variety of subjects. First of all, I am starting an internship in a histology lab and will try to perfect decalcification of tissues from different types of embalming methods. My fisrt problem is the embedding center, a Tissue Tek II ( http://www.medical-equipment-classifieds.com/classifieds/view-ad-2535.html). At first, even at the coldest setting, paraffin went up to 85-90oC. We played with the intern screw around the thermostat, and I think we just "screwed" it up even more and the fuse blew... smoke was coming out of the control box. So, if anyone has the user's manual to this particular machine, it would be appreciated. Do you have a method to embed tissue without an embedding center? The cooling plate works fine; it is just the paraffin bath is not heating anymore. Sakura does not offer help for this machine anymore. In the same line of idea, if anyone has the manual for the Tissue Tek VIP 2000, model 4618 circulator, would be appreciated as well. I will be trying an HCl solution 0,5N in water as a decalcification agent. Protocols suggest a neutralisation step afterwards of a 12h bath in sodium sulfate or lithium sulfate. I don't have neither of these products, but have sodium sulfite or magnesium sulfate. Would a bath in one of these work the same, or is a bath under running water equivalent to this step of neutralisation? I have also a question about decalcification of bones/teeth versus non-calcified tissue, like liver/kidney/muscles. Is EDTA less harsh for these tissues than acid-based decalcifiers? I will not be working with hardly calcified tissues, but more with soft ones. Hope that is not too much, I am just an eager student excited to get into the world of science research. Thank you, Julien From Allison.Scott <@t> harrishealth.org Thu Jan 29 13:44:18 2015 From: Allison.Scott <@t> harrishealth.org (Scott, Allison D) Date: Thu Jan 29 13:44:28 2015 Subject: [Histonet] Tissue Processor Validation Procedure Message-ID: Hello to all in Histoland. Does anyone have a tissue processor validation procedure that they would be willing to share? Any help in this would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From craigak12 <@t> gmail.com Thu Jan 29 14:24:50 2015 From: craigak12 <@t> gmail.com (Jb) Date: Thu Jan 29 14:24:56 2015 Subject: [Histonet] Her2: Message-ID: <48C30FFE-B449-42C2-AD31-B7169C8002FE@gmail.com> What her2 products are most labs using? Does anyone know of good FDA approved Her2 antibody? Are most labs using FDA approved Her2 or ASR? Thank you for your help, Sent from my iPhone From GKeyser <@t> uwhealth.org Fri Jan 30 08:47:11 2015 From: GKeyser <@t> uwhealth.org (Keyser Gerald T) Date: Fri Jan 30 08:47:17 2015 Subject: [Histonet] Covergrip Message-ID: <5226C88C65EBFF4BAD552D68DC6E8FFE042972@UWHC-MBX12.uwhis.hosp.wisc.edu> I would like to better seal my Oil Red O slides. The current process uses aqueous mountant on the inside of the large coverslips and Permount on the outside; which is incredibly messy and isn't actually that great of a seal. The two options I'm looking at is: 1) Nail polish. 2) Covergrip I would like to get away with using nail polish because it's 10% of the cost of Covergrip. We don't actually do a whole lot of Oil Red O stains. But, I'm a little concerned that the solvents in the nail polish may permeate the aqueous mountant and mess with the sections. Slides need to be good for 10 years. Opinions? Gerry From contact <@t> excaliburpathology.com Fri Jan 30 09:26:45 2015 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Jan 30 09:26:52 2015 Subject: [Histonet] Covergrip In-Reply-To: <5226C88C65EBFF4BAD552D68DC6E8FFE042972@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <5226C88C65EBFF4BAD552D68DC6E8FFE042972@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: <1184693201.3003445.1422631605067.JavaMail.yahoo@mail.yahoo.com> I have used Crystal Mount for the last 15-20 years for coverslipping Oil Red O, AEC, and other stains needing aqueous mounting media. Biomeda went out of business a few years ago, but this is a very good substitute. ClearMount in PIPES Buffer. Adhesives and Mountants: Chemicals for Light Microscopy, Histology and Electron Microscopy Place a few drops on the wet section, let it completely dry, then coverslip with regular resin mounting media. ClearMount forms an impervious barrier to the xylene based media and the slides do last for years. ??Paula Pierce,BS, HTL(ASCP)HT?President?Excalibur Pathology, Inc.?5830 N Blue Lake Dr.?Norman, OK 73069?405-759-3953 PH 405-759-7513 FAX?www.excaliburpathology.com From: Keyser Gerald T To: "'histonet@lists.utsouthwestern.edu'" Sent: Friday, January 30, 2015 8:47 AM Subject: [Histonet] Covergrip I would like to better seal my Oil Red O slides. The current process uses aqueous mountant on the inside of the large coverslips and Permount on the outside; which is incredibly messy and isn't actually that great of a seal. The two options I'm looking at is: 1)? ? ? Nail polish. 2)? ? ? Covergrip I would like to get away with using nail polish because it's 10% of the cost of Covergrip. We don't actually do a whole lot of Oil Red O stains.? But, I'm a little concerned that the solvents in the nail polish may permeate the aqueous mountant and mess with the sections. Slides need to be good for 10 years. Opinions? Gerry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Fri Jan 30 09:40:48 2015 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Jan 30 09:40:56 2015 Subject: [Histonet] Covergrip In-Reply-To: <5226C88C65EBFF4BAD552D68DC6E8FFE042972@UWHC-MBX12.uwhis.hosp.wisc.edu> References: <5226C88C65EBFF4BAD552D68DC6E8FFE042972@UWHC-MBX12.uwhis.hosp.wisc.edu> Message-ID: <1428655105.3006785.1422632448546.JavaMail.yahoo@mail.yahoo.com> Looks like the like to EMS did not post correctly. This is the link to the datasheet for ClearMount. Clear-Mount, with Tris Buffer or Pipes Buffer Technical Data Sheet | ? | | ? | ? | ? | ? | ? | | Clear-Mount, with Tris Buffer or Pipes Buffer Technical Data SheetTechnical data sheet from Electron Microscopy Sciences | | | | View on www.emsdiasum.com | Preview by Yahoo | | | | ? | ??Paula? From: Keyser Gerald T To: "'histonet@lists.utsouthwestern.edu'" Sent: Friday, January 30, 2015 8:47 AM Subject: [Histonet] Covergrip I would like to better seal my Oil Red O slides. The current process uses aqueous mountant on the inside of the large coverslips and Permount on the outside; which is incredibly messy and isn't actually that great of a seal. The two options I'm looking at is: 1)? ? ? Nail polish. 2)? ? ? Covergrip I would like to get away with using nail polish because it's 10% of the cost of Covergrip. We don't actually do a whole lot of Oil Red O stains.? But, I'm a little concerned that the solvents in the nail polish may permeate the aqueous mountant and mess with the sections. Slides need to be good for 10 years. Opinions? Gerry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kathy.Machado <@t> LPNT.net Fri Jan 30 13:57:25 2015 From: Kathy.Machado <@t> LPNT.net (Kathy.Machado@LPNT.net) Date: Fri Jan 30 13:57:43 2015 Subject: [Histonet] small lab fte's Message-ID: Dianna, I also work in a small lab, with three histotechs. The hours are 5:00 am to 5:00 pm. We need three people to cover these hours. That is how we justify it. Hope this helps. Kathy Machado, HTL Danville Regional Medical Center Danville, VA Kathy.Machado@lpnt.net 434-799-3867 From cjohnson <@t> nmda.nmsu.edu Fri Jan 30 14:18:56 2015 From: cjohnson <@t> nmda.nmsu.edu (Johnson, Carole) Date: Fri Jan 30 14:19:02 2015 Subject: [Histonet] Tissue Processor Validation Procedure Message-ID: Hello Allison, When we purchased a new processor, I chose tissue specimens representative of what we normally see in our lab (surgical/biopsy and necropsy tissues) from cases which had been signed out within the previous 48-72 hours. I then ran parallel samples on both processors (same protocols, run the same day, fresh reagents in both processors) and then set up a grading scale for the pathologists to do a blind comparison. After that, I did a (brief) statistical analysis to determine if there was any significant difference between slides from the two processors. For IHC (we are a small lab with a short IHC menu currently), I ran parallel Vimentin and Multicytokeratin on the same blocks as used for the H&E and did a comparison just as for the H&E slides. I presented the final data to my chief pathologist who then signed off on the validation. I keep a copy of the validation with my equipment records. I hope this helps. Best regards, Carole Carole Johnson Carole Johnson, HT(ASCP)cm New Mexico Department of Agriculture Veterinary Diagnostic Services 505.383.9299 To understand is to stand under, which is to look up, which is a good way to understand Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. From Ronald.Houston <@t> nationwidechildrens.org Fri Jan 30 15:04:00 2015 From: Ronald.Houston <@t> nationwidechildrens.org (Houston, Ronald) Date: Fri Jan 30 15:04:30 2015 Subject: [Histonet] VEGF3 and VEGF Message-ID: Can you please tell me which antibody sources you have had best results for these epitopes? we've been getting inconsistent results with the ones we've tried Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.houston@nationwidechildrens.org www.NationwideChildrens.org "One person with passion is better than forty people merely interested." ~ E.M. Forster From j.rowaihi <@t> alborglaboratories.com Sat Jan 31 05:49:05 2015 From: j.rowaihi <@t> alborglaboratories.com (Jamal) Date: Sat Jan 31 05:49:31 2015 Subject: [Histonet] Tissue Processor Validation Procedure In-Reply-To: References: Message-ID: <000e01d03d4b$f01e8d40$d05ba7c0$@rowaihi@alborglaboratories.com> Hi Allison I hope the attached can help you Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories |? Mobile +966 503629832| j.rowaihi@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA | Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, January 29, 2015 10:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Validation Procedure Hello to all in Histoland. Does anyone have a tissue processor validation procedure that they would be willing to share? Any help in this would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdwyer3322 <@t> aol.com Sat Jan 31 09:38:11 2015 From: kdwyer3322 <@t> aol.com (kdwyer3322@aol.com) Date: Sat Jan 31 09:38:19 2015 Subject: [Histonet] "Texas Histology: A Wealth of Knowledge" Texas Society for Histotechnology Annual Symposium/Convention Dallas/Plano Marriott at Legacy TownCenter,Plano, Texas-March 20-22, 2015 Message-ID: <8D20B830DB668C2-610-5BE8@webmail-vm130.sysops.aol.com> Hi Histonetters! The Texas Society for Histotechnology will be holding the 2015 S/C in Plano Texas. If you are interested in a electronic version of the program please send me a e-mail via this communication. It is not too late to sig up. We would love to have you join us this year! Regards, Kathy Dwyer Take a look at the great symposiums and workshop we have to offer! Take Control of Your Controls- HeatherRenko Understanding the Business ofPathology and How It Affects Your AP Laboratory- Loretta Sayles The Science of Fixation andProcessing- Jan Minshew H. Pylori: Special Staining Methodsand Troubleshooting - Ranna Mehta/Debbie Siena Cool Tips for Cryostat Users - Jan Minshew Resume Wriitng and InterviewPreparation ? JanaE Mitchell/ Amy Plewinski Optimization of Microtomy - Past,Present and the 21st Century -H. Skip. Brown Unmasking IHC and MolecularTechniques ? Brent Hart A Pratical Approach For TheHistological Evaluation of Underminerlized Bone, synthetic Biomaterial andMedical Devie Implants ? Jack Ratliff A Critical Component of AutopyPathology ? Shemeika Johnson From Mouse to Microscope - Jennifer Johnson Understanding the Characteristics ofStains and Dyes and Their Importance in the Histology Laboratory ? Debbie Siena/Gary Weiderhold Guidelines for Studing Special Stainsin preparation for the HT Examination ? Rayan Gonzalez/Lin Bustamante Capital Budgeting - Olga Kochar Streamling the Path Line ? Kelsi Currier/CarolBethTaylor /Ary Franklin Introduction to ISH and Case Studies ?Heather Renko The Pathologist Said: "I haveAchromotyfil" and You Said" What?Is That" ? Pamela Marcum Quality Management- Yesterday, Todayand Tomorrow-Olga Kochar Fundamental IHC Technique and Theoriesof In situ Hybridization with a Comparison to Immunohistochemistry-Theresa Burchette Oh My! What Does CLIA and CAP WantDuring and After A Inspection ? Debbie Siena/Kathy Dwyer Technological Advancements inMicrotomy:A Non-Contact Alternative to Conventional Histology Equipment andTechniques- Jack Ratliff Leadership: Theory to Application ? JenniferNelson